LOCUS       DAJTVE010000001        88069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000001 DAJTVE010000000
VERSION     DAJTVE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO439_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO439_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6314491.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO439_000002"
     tRNA            1230..1305
                     /locus_tag="OO439_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OO439_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OO439_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OO439_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OO439_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6314492.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OO439_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OO439_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6314493.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OO439_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OO439_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6314494.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="OO439_000007"
     CDS             complement(4869..5306)
                     /locus_tag="OO439_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6314495.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="OO439_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="OO439_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OO439_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OO439_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6314496.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="OO439_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="OO439_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6314497.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="OO439_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="OO439_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6314498.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="OO439_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="OO439_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6314499.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="OO439_000013"
     CDS             10774..11442
                     /locus_tag="OO439_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6314500.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="OO439_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="OO439_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6314501.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="OO439_000015"
     CDS             11985..13817
                     /locus_tag="OO439_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6314502.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="OO439_000016"
     CDS             13807..14157
                     /locus_tag="OO439_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6314503.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="OO439_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="OO439_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6314504.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="OO439_000018"
     CDS             14476..14898
                     /locus_tag="OO439_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6314505.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="OO439_000019"
     CDS             14901..15533
                     /locus_tag="OO439_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6314506.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="OO439_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="OO439_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6314507.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="OO439_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="OO439_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6314508.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="OO439_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="OO439_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6314509.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="OO439_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="OO439_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="OO439_000024"
     CDS             18712..19824
                     /locus_tag="OO439_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6314510.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="OO439_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="OO439_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="OO439_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="OO439_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6314511.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="OO439_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="OO439_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6314512.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="OO439_000028"
     CDS             22078..22521
                     /locus_tag="OO439_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6314513.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="OO439_000029"
     CDS             complement(22622..22951)
                     /locus_tag="OO439_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6314514.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="OO439_000030"
     CDS             complement(23123..24181)
                     /locus_tag="OO439_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6314515.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24394..24852)
                     /gene="sbmC"
                     /locus_tag="OO439_000031"
     CDS             complement(24394..24852)
                     /gene="sbmC"
                     /locus_tag="OO439_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6314516.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAV"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OO439_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OO439_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6314517.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="OO439_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="OO439_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6314518.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="OO439_000034"
     CDS             complement(27816..28043)
                     /locus_tag="OO439_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6314519.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OO439_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OO439_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6314520.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OO439_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OO439_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6314521.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OO439_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OO439_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6314522.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="OO439_000038"
     CDS             complement(30919..31848)
                     /locus_tag="OO439_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6314523.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="OO439_000039"
     CDS             complement(31894..32718)
                     /locus_tag="OO439_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6314524.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OO439_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OO439_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6314525.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OO439_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OO439_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6314526.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="OO439_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="OO439_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6314527.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33612..33735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="OO439_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="OO439_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6314528.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="OO439_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="OO439_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6314529.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="OO439_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="OO439_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6314530.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /gene="hisB"
                     /locus_tag="OO439_000046"
     CDS             37058..38125
                     /gene="hisB"
                     /locus_tag="OO439_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6314531.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="OO439_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="OO439_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6314532.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="OO439_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="OO439_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6314533.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="OO439_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="OO439_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6314534.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="OO439_000050"
     CDS             40204..40815
                     /locus_tag="OO439_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6314535.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OO439_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OO439_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6314536.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OO439_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OO439_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6314537.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OO439_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OO439_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6314538.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OO439_000054"
                     /gene_synonym="manB"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OO439_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6314539.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OO439_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OO439_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6314540.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OO439_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OO439_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6314541.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OO439_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OO439_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6314542.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OO439_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OO439_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6314543.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OO439_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OO439_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6314544.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OO439_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OO439_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6314545.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OO439_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OO439_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6314546.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OO439_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OO439_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6314547.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OO439_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OO439_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6314548.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OO439_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OO439_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6314549.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OO439_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OO439_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6314550.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OO439_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OO439_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6314551.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OO439_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OO439_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6314552.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OO439_000068"
     CDS             complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OO439_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6314553.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60964..61113
                     /locus_tag="OO439_000069"
     CDS             60964..61113
                     /locus_tag="OO439_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314554.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61314..62897
                     /locus_tag="OO439_000070"
     CDS             61314..62897
                     /locus_tag="OO439_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6314555.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OO439_000071"
     CDS             complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OO439_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6314556.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OO439_000072"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OO439_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6314557.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OO439_000073"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OO439_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6314558.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="OO439_000074"
     CDS             66678..69995
                     /locus_tag="OO439_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6314559.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OO439_000075"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OO439_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6314560.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="OO439_000076"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="OO439_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6314561.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OO439_000077"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OO439_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6314562.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OO439_000078"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OO439_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6314563.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="OO439_000079"
     CDS             complement(75147..75806)
                     /locus_tag="OO439_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6314564.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OO439_000080"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OO439_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6314565.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="OO439_000081"
     CDS             complement(76359..76415)
                     /locus_tag="OO439_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6314566.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="OO439_000082"
     CDS             complement(76686..76742)
                     /locus_tag="OO439_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6314567.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="OO439_000083"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="OO439_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6314568.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="OO439_000084"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="OO439_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6314569.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="OO439_000085"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="OO439_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6314570.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="OO439_000086"
     CDS             84463..85878
                     /locus_tag="OO439_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6314571.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..87278
                     /gene="baeS"
                     /locus_tag="OO439_000087"
     CDS             85875..87278
                     /gene="baeS"
                     /locus_tag="OO439_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6314572.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87275..87997
                     /gene="baeR"
                     /locus_tag="OO439_000088"
     CDS             87275..87997
                     /gene="baeR"
                     /locus_tag="OO439_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6314573.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21559 a        23313 c        22145 g        21052 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcctgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatt gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctacacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac caacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    87181 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    87241 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    87301 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    87361 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    87421 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    87481 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    87541 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    87601 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    87661 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    87721 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    87781 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    87841 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    87901 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    87961 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    88021 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggt
//
LOCUS       DAJTVE010000002        79580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000002 DAJTVE010000000
VERSION     DAJTVE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OO439_000089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OO439_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OO439_000090"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OO439_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6314574.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OO439_000091"
     CDS             2354..2758
                     /locus_tag="OO439_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6314575.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OO439_000092"
     CDS             2811..2921
                     /locus_tag="OO439_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314576.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OO439_000093"
     CDS             3157..3201
                     /locus_tag="OO439_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314577.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OO439_000094"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OO439_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6314578.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO439_000095"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO439_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6314579.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OO439_000096"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OO439_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6314580.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OO439_000097"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OO439_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6314581.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OO439_000098"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OO439_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6314582.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OO439_000099"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OO439_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6314583.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OO439_000100"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OO439_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6314584.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OO439_000101"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OO439_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6314585.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OO439_000102"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OO439_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6314586.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OO439_000103"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OO439_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6314587.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO439_000104"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO439_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6314588.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OO439_000105"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OO439_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6314589.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OO439_000106"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OO439_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6314590.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OO439_000107"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OO439_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6314591.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OO439_000108"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OO439_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6314592.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO439_000109"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO439_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6314593.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO439_000110"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO439_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6314594.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO439_000111"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO439_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6314595.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO439_000112"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO439_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6314596.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO439_000113"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO439_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6314597.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OO439_000114"
     CDS             23801..24874
                     /locus_tag="OO439_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6314598.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="OO439_000115"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="OO439_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6314599.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="OO439_000116"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="OO439_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO439_000117"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO439_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6314600.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="OO439_000118"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="OO439_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6314601.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="OO439_000119"
     CDS             complement(30823..31362)
                     /locus_tag="OO439_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6314602.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO439_000120"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO439_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6314603.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="OO439_000121"
     CDS             complement(33301..33843)
                     /locus_tag="OO439_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6314604.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO439_000122"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO439_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6314605.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO439_000123"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO439_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6314606.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO439_000124"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO439_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6314607.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="OO439_000125"
     CDS             complement(36362..36739)
                     /locus_tag="OO439_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6314608.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO439_000126"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO439_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6314609.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="OO439_000127"
                     /pseudo
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="OO439_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO439_000128"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO439_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6314610.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="OO439_000129"
     CDS             complement(41412..42209)
                     /locus_tag="OO439_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6314611.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO439_000130"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO439_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6314612.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="OO439_000131"
     CDS             complement(43221..43862)
                     /locus_tag="OO439_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6314613.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO439_000132"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO439_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6314614.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO439_000133"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO439_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6314615.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO439_000134"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO439_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6314616.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO439_000135"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO439_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6314617.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO439_000136"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO439_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6314618.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO439_000137"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO439_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6314619.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO439_000138"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO439_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6314620.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO439_000139"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO439_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6314621.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO439_000140"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO439_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6314622.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO439_000141"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO439_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6314623.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="OO439_000142"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="OO439_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6314624.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO439_000143"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO439_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6314625.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="OO439_000144"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="OO439_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6314626.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO439_000145"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO439_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6314627.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO439_000146"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO439_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6314628.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO439_000147"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO439_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6314629.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO439_000148"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO439_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6314630.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO439_000149"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO439_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6314631.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO439_000150"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO439_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO439_000151"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO439_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6314632.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO439_000152"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO439_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6314633.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO439_000153"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO439_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6314634.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO439_000154"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO439_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6314635.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO439_000155"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO439_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6314636.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="OO439_000156"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="OO439_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6314637.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="OO439_000157"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="OO439_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6314638.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="OO439_000158"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="OO439_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6314639.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO439_000159"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO439_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6314640.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="OO439_000160"
     CDS             complement(71243..72166)
                     /locus_tag="OO439_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6314641.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="OO439_000161"
     CDS             complement(72168..72815)
                     /locus_tag="OO439_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6314642.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO439_000162"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO439_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6314643.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="OO439_000163"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="OO439_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6314644.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="OO439_000164"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="OO439_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6314645.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="OO439_000165"
     CDS             75699..76259
                     /locus_tag="OO439_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6314646.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="OO439_000166"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="OO439_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6314647.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="OO439_000167"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="OO439_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6314648.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="OO439_000168"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="OO439_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6314649.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="OO439_000169"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="OO439_000169"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6314650.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19597 a        20912 c        19710 g        19361 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctag
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgatg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctcat
//
LOCUS       DAJTVE010000003        74165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000003 DAJTVE010000000
VERSION     DAJTVE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..814
                     /locus_tag="OO439_000170"
     CDS             <1..814
                     /locus_tag="OO439_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6314651.1"
                     /translation="VVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDP
                     LTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTK
                     LGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCT
                     FVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGAN
                     PSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            850..1476
                     /gene="mlrA"
                     /locus_tag="OO439_000171"
     CDS             850..1476
                     /gene="mlrA"
                     /locus_tag="OO439_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6314652.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSA"
     gene            1596..1703
                     /locus_tag="OO439_000172"
     CDS             1596..1703
                     /locus_tag="OO439_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6314653.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1684..2415)
                     /gene="yehW"
                     /locus_tag="OO439_000173"
     CDS             complement(1684..2415)
                     /gene="yehW"
                     /locus_tag="OO439_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6314654.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2420..3346)
                     /gene="yehX"
                     /locus_tag="OO439_000174"
     CDS             complement(2420..3346)
                     /gene="yehX"
                     /locus_tag="OO439_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6314655.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3339..4496)
                     /gene="yehY"
                     /locus_tag="OO439_000175"
     CDS             complement(3339..4496)
                     /gene="yehY"
                     /locus_tag="OO439_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6314656.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4503..5420)
                     /gene="osmF"
                     /locus_tag="OO439_000176"
     CDS             complement(4503..5420)
                     /gene="osmF"
                     /locus_tag="OO439_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6314657.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5672..7969)
                     /gene="bglX"
                     /locus_tag="OO439_000177"
     CDS             complement(5672..7969)
                     /gene="bglX"
                     /locus_tag="OO439_000177"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6314658.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8165..9880
                     /gene="dld"
                     /locus_tag="OO439_000178"
     CDS             8165..9880
                     /gene="dld"
                     /locus_tag="OO439_000178"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6314659.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9919..10851)
                     /gene="pbpG"
                     /locus_tag="OO439_000179"
     CDS             complement(9919..10851)
                     /gene="pbpG"
                     /locus_tag="OO439_000179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6314660.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11025..11612)
                     /locus_tag="OO439_000180"
     CDS             complement(11025..11612)
                     /locus_tag="OO439_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6314661.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11782..12360
                     /locus_tag="OO439_000181"
     CDS             11782..12360
                     /locus_tag="OO439_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6314662.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12490..13251)
                     /locus_tag="OO439_000182"
     CDS             complement(12490..13251)
                     /locus_tag="OO439_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6314663.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13304..14740)
                     /gene="mdtQ"
                     /locus_tag="OO439_000183"
     CDS             complement(13304..14740)
                     /gene="mdtQ"
                     /locus_tag="OO439_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6314664.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            14964..15047
                     /gene="yohP"
                     /locus_tag="OO439_000184"
     CDS             14964..15047
                     /gene="yohP"
                     /locus_tag="OO439_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6314665.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15420..16367)
                     /gene="dusC"
                     /locus_tag="OO439_000185"
     CDS             complement(15420..16367)
                     /gene="dusC"
                     /locus_tag="OO439_000185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6314666.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16606..17004
                     /locus_tag="OO439_000186"
     CDS             16606..17004
                     /locus_tag="OO439_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6314667.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17001..17696
                     /locus_tag="OO439_000187"
     CDS             17001..17696
                     /locus_tag="OO439_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6314668.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17826..18710
                     /gene="cdd"
                     /locus_tag="OO439_000188"
     CDS             17826..18710
                     /gene="cdd"
                     /locus_tag="OO439_000188"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6314669.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18860..19579
                     /gene="sanA"
                     /locus_tag="OO439_000189"
     CDS             18860..19579
                     /gene="sanA"
                     /locus_tag="OO439_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6314670.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19582..19821
                     /locus_tag="OO439_000190"
     CDS             19582..19821
                     /locus_tag="OO439_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6314671.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20015..21253
                     /gene="preT"
                     /locus_tag="OO439_000191"
                     /pseudo
     CDS             20015..21253
                     /gene="preT"
                     /locus_tag="OO439_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21247..22482
                     /gene="preA"
                     /locus_tag="OO439_000192"
     CDS             21247..22482
                     /gene="preA"
                     /locus_tag="OO439_000192"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6314672.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22609..23619)
                     /gene="mglC"
                     /locus_tag="OO439_000193"
     CDS             complement(22609..23619)
                     /gene="mglC"
                     /locus_tag="OO439_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6314673.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23635..25155)
                     /gene="mglA"
                     /locus_tag="OO439_000194"
     CDS             complement(23635..25155)
                     /gene="mglA"
                     /locus_tag="OO439_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6314674.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25216..26214)
                     /gene="mglB"
                     /locus_tag="OO439_000195"
     CDS             complement(25216..26214)
                     /gene="mglB"
                     /locus_tag="OO439_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6314675.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26494..27534)
                     /gene="galS"
                     /locus_tag="OO439_000196"
                     /pseudo
     CDS             complement(26494..27534)
                     /gene="galS"
                     /locus_tag="OO439_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27676..28833)
                     /locus_tag="OO439_000197"
     CDS             complement(27676..28833)
                     /locus_tag="OO439_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6314676.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28850..29518)
                     /gene="folE"
                     /locus_tag="OO439_000198"
     CDS             complement(28850..29518)
                     /gene="folE"
                     /locus_tag="OO439_000198"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6314677.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVIIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29776..30612
                     /gene="yeiG"
                     /locus_tag="OO439_000199"
     CDS             29776..30612
                     /gene="yeiG"
                     /locus_tag="OO439_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6314678.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30644..32635)
                     /gene="cirA"
                     /locus_tag="OO439_000200"
     CDS             complement(30644..32635)
                     /gene="cirA"
                     /locus_tag="OO439_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6314679.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(32927..34396)
                     /gene="lysP"
                     /locus_tag="OO439_000201"
     CDS             complement(32927..34396)
                     /gene="lysP"
                     /locus_tag="OO439_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6314680.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34601..35482)
                     /locus_tag="OO439_000202"
     CDS             complement(34601..35482)
                     /locus_tag="OO439_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6314681.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35581..36630
                     /locus_tag="OO439_000203"
     CDS             35581..36630
                     /locus_tag="OO439_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6314682.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36704..37561
                     /gene="nfo"
                     /locus_tag="OO439_000204"
     CDS             36704..37561
                     /gene="nfo"
                     /locus_tag="OO439_000204"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6314683.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37565..38653
                     /locus_tag="OO439_000205"
     CDS             37565..38653
                     /locus_tag="OO439_000205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6314684.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38709..39959)
                     /locus_tag="OO439_000206"
     CDS             complement(38709..39959)
                     /locus_tag="OO439_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6314685.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40059..41000)
                     /gene="rihB"
                     /locus_tag="OO439_000207"
     CDS             complement(40059..41000)
                     /gene="rihB"
                     /locus_tag="OO439_000207"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6314686.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41130..41828
                     /gene="yeiL"
                     /locus_tag="OO439_000208"
     CDS             41130..41828
                     /gene="yeiL"
                     /locus_tag="OO439_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6314687.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(41895..43145)
                     /locus_tag="OO439_000209"
     CDS             complement(41895..43145)
                     /locus_tag="OO439_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6314688.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43239..44177)
                     /gene="psuG"
                     /locus_tag="OO439_000210"
     CDS             complement(43239..44177)
                     /gene="psuG"
                     /locus_tag="OO439_000210"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6314689.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44165..45106)
                     /locus_tag="OO439_000211"
     CDS             complement(44165..45106)
                     /locus_tag="OO439_000211"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6314690.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45564..47255)
                     /gene="fruA"
                     /locus_tag="OO439_000212"
     CDS             complement(45564..47255)
                     /gene="fruA"
                     /locus_tag="OO439_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6314691.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47272..48210)
                     /gene="fruK"
                     /locus_tag="OO439_000213"
     CDS             complement(47272..48210)
                     /gene="fruK"
                     /locus_tag="OO439_000213"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6314692.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48210..49340)
                     /gene="fruB"
                     /locus_tag="OO439_000214"
     CDS             complement(48210..49340)
                     /gene="fruB"
                     /locus_tag="OO439_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6314693.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49708..50889
                     /gene="setB"
                     /locus_tag="OO439_000215"
     CDS             49708..50889
                     /gene="setB"
                     /locus_tag="OO439_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6314694.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(50886..51140)
                     /locus_tag="OO439_000216"
     CDS             complement(50886..51140)
                     /locus_tag="OO439_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6314695.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51295..51867
                     /gene="yeiP"
                     /locus_tag="OO439_000217"
     CDS             51295..51867
                     /gene="yeiP"
                     /locus_tag="OO439_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6314696.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52090..53556
                     /locus_tag="OO439_000218"
     CDS             52090..53556
                     /locus_tag="OO439_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6314697.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53674..54660
                     /gene="yeiR"
                     /locus_tag="OO439_000219"
     CDS             53674..54660
                     /gene="yeiR"
                     /locus_tag="OO439_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6314698.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54699..55412
                     /gene="lpxT"
                     /locus_tag="OO439_000220"
     CDS             54699..55412
                     /gene="lpxT"
                     /locus_tag="OO439_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6314699.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55824..56390
                     /gene="mepS"
                     /locus_tag="OO439_000221"
     CDS             55824..56390
                     /gene="mepS"
                     /locus_tag="OO439_000221"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6314700.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56571..58127
                     /locus_tag="OO439_000222"
     CDS             56571..58127
                     /locus_tag="OO439_000222"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6314701.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58209..60023
                     /locus_tag="OO439_000223"
     CDS             58209..60023
                     /locus_tag="OO439_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6314702.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60024..61118
                     /locus_tag="OO439_000224"
     CDS             60024..61118
                     /locus_tag="OO439_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6314703.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61118..62143
                     /locus_tag="OO439_000225"
     CDS             61118..62143
                     /locus_tag="OO439_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6314704.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62145..63734
                     /gene="yejF"
                     /locus_tag="OO439_000226"
     CDS             62145..63734
                     /gene="yejF"
                     /locus_tag="OO439_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6314705.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63738..64082)
                     /locus_tag="OO439_000227"
     CDS             complement(63738..64082)
                     /locus_tag="OO439_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314706.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64415..65535)
                     /locus_tag="OO439_000228"
                     /pseudo
     CDS             complement(64415..65535)
                     /locus_tag="OO439_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65563..66258)
                     /gene="rsuA"
                     /locus_tag="OO439_000229"
     CDS             complement(65563..66258)
                     /gene="rsuA"
                     /locus_tag="OO439_000229"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6314707.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66408..68168
                     /locus_tag="OO439_000230"
     CDS             66408..68168
                     /locus_tag="OO439_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6314708.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68293..68577
                     /gene="rplY"
                     /locus_tag="OO439_000231"
     CDS             68293..68577
                     /gene="rplY"
                     /locus_tag="OO439_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6314709.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68716..69723)
                     /gene="yejK"
                     /locus_tag="OO439_000232"
     CDS             complement(68716..69723)
                     /gene="yejK"
                     /locus_tag="OO439_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6314710.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            69905..70132
                     /locus_tag="OO439_000233"
     CDS             69905..70132
                     /locus_tag="OO439_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6314711.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70152..71912
                     /gene="pbgA"
                     /locus_tag="OO439_000234"
     CDS             70152..71912
                     /gene="pbgA"
                     /locus_tag="OO439_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6314712.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            71987..72063
                     /locus_tag="OO439_000235"
     tRNA            71987..72063
                     /locus_tag="OO439_000235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72021..72023,aa:Pro,seq:ggg)
     gene            complement(72166..74165)
                     /gene="yejO"
                     /locus_tag="OO439_000236"
                     /pseudo
     CDS             complement(72166..74165)
                     /gene="yejO"
                     /locus_tag="OO439_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18198 a        19555 c        18214 g        18198 t
ORIGIN      
        1 agtggtcaca gtaaactact atatgacaac catagctgga atgtttcaat ttgcaacaga
       61 taatggttat acatcaggaa atccatttaa cggtctgaca ccattaaaaa agtccaagat
      121 agaaccagat cccctcaccc gtgacgaatt tattcgtttt attgaggctt gccgtcatca
      181 acaaacaaaa aacctgtgga ttatcgctgt atacacgggt attcgtcacg gggagctggt
      241 atcactggcc tgggaagata tagaccttaa agcgaggact ataaccatcc gccggaatta
      301 tacaaaacta ggcgaattca ctccaccaaa aactgatgct ggtaccggaa gaacgattca
      361 tctagttcaa ccagctattg atgctcttaa aagccaagcg gaaatgacca tgcttggaaa
      421 acagcattct gtagaggtaa agcagaggga atatgggaga agtactgtgc ataaatgcac
      481 ttttgttttt agtcctcagg taataaaaca gcggcagttt tccggaccgc actataaggt
      541 tgactccatc agggagtcat ggacaagtat cttaaaacgc gcaggtctga gacacagaaa
      601 atcgtaccaa tccaggcata cttatgcatg ctggtcactt gccgctggag ctaatcctag
      661 ttttatcgca agccagatgg gccacacaaa cgcacaaatg gtattcaatg tttacggagc
      721 atggatgaaa gacaacaatc acgaacagat agaactcctt aacaaaagac tatctgaaag
      781 tgtcccatgt atgccccata agaaagtggg gtaaaataaa aacttgtaaa atcagttagt
      841 ttacccttaa tccctgtcac gttacgcgcg tggcagaggc gttacgggtt gctgaaaccg
      901 caacggacag acggcggtca tcgactgttc aacgatgccg atattgaccg gatccgcgag
      961 atcaaacgct ggatcgacaa cggcgtgcag gtcagcaaag ttaaaatgct gctcagtaat
     1021 gaaaatgttg atgtgcagaa cggctggcgc gatcagcaag aaacattact gacctacctg
     1081 caaagcggca atctgcatag cctgcgaacg tggatcaaag agcgcggtca ggattacccc
     1141 gcccagacac tcaccacaca tctgtttatt cctctgcgcc gacggcttca gtgccaacaa
     1201 ccgactctcc aggcgctgct ggcgatcctc gacggcgtac tgatcaacta catcgccatt
     1261 tgtctggctt cggcacgtaa aaaacagggt aaagatgcgc tggtggttgg ctggaatatt
     1321 caggatacca cccgtctgtg gctggagggc tggattgcca gtcaacaagg atggcgcatt
     1381 gatgtcctcg cccactcgct caatcaacta cgccctgaac tattcgaagg ccgtacattg
     1441 ctggtgtggt gcggtgaaaa tcgaacctcc gcctaacagc agcaactcac cagttggcaa
     1501 gaacaaggcc atgatatttt cccactcggc atttaatgat tcgttaacaa atgcgcttta
     1561 ctgtacaatc ctttcgttaa cataaggagt gcaatatgcg catagctaaa attggggtca
     1621 tcgccctgtt cctgtttatg gcgttaggcg gaattggtgg cgtcatgctc gcaggttata
     1681 cctttatttt gcgtgctggc taagcgcctg caccagcctt tcaaacaggc ggtctgcgat
     1741 gatcgccgcc agtgccacca gtaacgcccc ctggatcaca tacgcggtat taaatccgct
     1801 aagcccgatg atgatgggcg tacccagcgt gctggcccct accgttgagg cgatcgtcgc
     1861 cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc agaatcaccg gagccgccag
     1921 cggtagctcg accttacgca gtcgctgacc acgactcatt cccatacctt tcgcaacttc
     1981 tgtcacgctg gcatcaatcg ctcccagccc ggcaagtgtc gcctgcagga cgggcagcac
     2041 accgtaaagg atcaaggcga taatcgctgg ttgcagacca aagccgatca ccggaacggc
     2101 aatcgccagc actgcgacag gcggaaaagt ctgtccaacg gcggcaatag tttccaccag
     2161 tgggcgaaat tccgcgcccc acgggcgagt gacagcaatt ccggcaccag tgccaatgat
     2221 caccgcaaac aaactcgaaa ttcccaccag ccagaaatga gccagtgcca gagctgcaaa
     2281 actttcttgc tgataaacgg gtcgtggcag ttgtgggaac aaggcagcaa acagcggctg
     2341 gctgtaaggc agccagaaaa tcagtgccac aaacaaagca atgagccaga acagcggatc
     2401 gcgcaacatc ttcatacgct tacgcctcct ccagcagatc ctgaaaatgc agcgtgccgc
     2461 aaggctggcc ctgcgtgttc accaccggca gcacctcgca tccccgcgcg acaaacaggg
     2521 agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc accttctgcc cgttcttcgc
     2581 gacgcacgta atccgccaca ctacgtaacg aaagcaggcg cacacccagt tcgctgcgcc
     2641 caaaaaactg gcggacaaaa tcgtttgccg gacgagtcag catcgtcagc ggattgccct
     2701 gctgcaccac ttcaccgtga tccatcaata ccagatgttc tgccagccgt agcgcctcat
     2761 caatatcatg agtgaccagc acaatggtac gccccagcaa acggtgaatg cgcgtcatct
     2821 cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc aaaaggttca tccatcagta
     2881 agacttgcgg atcggcagcc agtgcgcgcg ccactcccac acgttgctgc tgaccaccgg
     2941 aaagctgatg cggataacgc tcacgtaaat ttgactccag ccccagtagc gccattaatt
     3001 cgtcgatacg atcgtcaatc ctcgcccgcg accatttttg taattgcggc acggtagcga
     3061 tgttttgcgc cacgctccag tgggggaaca ggccaataga ttgaatggca tagcccatcc
     3121 ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc tccggcaaag cggatcactc
     3181 cgctgtcatg ctccaccagg cggttaatca ttttcagggt ggtggatttg ccggagccag
     3241 atgtgccaat cagcaccgaa aaactccctt cctgaaaatt gagattgaga tcgttaacgg
     3301 ctttttgtgc gccgaacagt ttgctgacat ggccaaattc aatcattacg tttcaccttc
     3361 agcagtgcga taagcaaatc gaacagcgcg tcggtaagca ccgccagaac aattaccggg
     3421 atcaccccca gcaacactaa atcaatggcg ctgcttagca gcccctggaa aaccagcgca
     3481 ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca tacctacagt ttgcaccatc
     3541 accacccgca ggctgcgcag aaataccggt aacgccagtg gtaactgaac atgcaggaat
     3601 cgctgcgccc cgctcatgcc catcgctctg gcgctctcca gcacatcgcg cgggatctgg
     3661 ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg catagagcac cagcgcaatc
     3721 agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc ccagccacgg aaaggccgta
     3781 accagcgcgg caagcggcgc aatcaacagg ccaaagagcg ccaccgaagg cacggtctga
     3841 atgacattga gcagagaaaa aattgccccc tgccgagcag tggaaaagta gcaccagatg
     3901 cccaacggca caccaatcac taacgcaggc agcaccgcac caaacagcaa cgtcagatgt
     3961 tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt attctttcat tagtgagaga
     4021 tcgttaagcg tgccggagta cagcaaccac agcggaataa tggcaatctg catatgcaac
     4081 aaccagcgcc acagcggatg cgtggagatt cggcggatgg catcactaca ggccagcaat
     4141 gccagcgccg cggccagcca gaaaccactg ccgaggctgg tacgcgccag cgcactgcca
     4201 ttttgcgcca gttgggtcgc cgcctttcct gctccccaca ccagcaatac gaagacgaat
     4261 tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg gaataaaaca ggctgtcagc
     4321 caggcgcaac caacgcccac cagcatccag atcgtttgcg gccacagttg ccagagatga
     4381 cgcccttcac ccgagactaa acgattaggc gcgtaactga taaacggcag cgccgctgcg
     4441 attgccgtca gcaacagcag cagcgccaga acaggattaa tacgtagata agtcacggga
     4501 aattacttcg tccacccttt ttgtttcagg tagtcggcag ccactttttt ggcatccagt
     4561 ccttccactg caatgctggc attcagttgc tgcaatgttt ttgcatcgag gctggcgaag
     4621 actggctgta gccactgtgc catttgcgga tactctttca gcaccgactc acgcaccact
     4681 ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc ttaaggtttg cagccccagc
     4741 gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat taacgccaga agtttgctgg
     4801 gcagcggctt taatcgttac cgccgtgtcg ccgccagcca gtgacagtaa ctgatcctga
     4861 tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg catcggcgcg ttcgataaac
     4921 tctgccgagg ctgccagttt gaaggtgccg ccctctttca gataacgact caggtcggca
     4981 agcgaagtga gtttgttttt ctctgccaca tcctgacgca cggcgatggt ccaggtgtta
     5041 ttcgctggtg cgggcgtcag ccagattaac ttgttttgct ctgcatcgag ttttttgact
     5101 ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt tttcatcttt aaagaagaaa
     5161 gcgccattac cggtatattc cggatagata tccagttcac cggaagtaat cgccccgcgc
     5221 accacaggag tcgttccgag ttgcacttta ttcaccgtcg gtacgccgtg gctttccagc
     5281 acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat cgatttttga accgacttta
     5341 acgggggaag ccgcctgtag cggcaggctc acggctgcca acataaccag tgaacctgcc
     5401 cagacctttg agagtggcat gatgctttcc tcattctttt actgttgttt tcagcgaatt
     5461 aagagaaaag catagttgat aatgacagag ttagcctgag cggaaggatt cagttgcaga
     5521 atcagataaa tacttaaagg gcatattttg cccggcgttt cttgccggat gcggcgtaaa
     5581 cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt aagatatgaa catcaggcaa
     5641 agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa ctcaaactcg cctttcttaa
     5701 cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc aggttcggcg tcatatttca
     5761 tctgttgatt ccagaacttc agcgcctcga tatcaatcgg gaagctgaca gtctgagttt
     5821 cgcccggttt cagggtgatt ttctcaaagc ctttcagctg cttcactgga cggctcatgg
     5881 aagccgtcac atcctgcaag tacatctgta ctaccgtggc cccttcgcgt ttaccggtgt
     5941 tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt catggtcggc gcagaaagtt
     6001 tcacatcaga gacggtgaaa gtggtatagc tcagaccata gccgaacgga taaagcgccc
     6061 cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg cttgtcggca ttatacggac
     6121 gaccggtatt cagatggctg tagtacaccg ggatctgccc gacagaacgc gggaaggaca
     6181 ttggcagctt gccggacggg ttgtaatcgc caaacaatac atcggcaatt gcattaccgc
     6241 cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc ctgctgatct tctttcacca
     6301 gcgccagcgg acgcccgttc atcagcacca gcaccagcgg tttaccggtg gctttcagcg
     6361 ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc ggtacggctg gaggcctcgt
     6421 gcgccatccc ctgtgcttca ccgaccacag ccaccaccac atcagattgt ttcgccgtct
     6481 gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc gactttgacc gcttcttcat
     6541 actgattcag gaaatcgata atgcctttgt cactggtaac gttcgcccct ttggcataca
     6601 gcactttacc gttttcaccg acggcatttt taatcccggt cagtacggtc acggattgat
     6661 cggcaacacc ggctgcggac cagctgccca tcacgtcacg tttactgtcc gccagtggcc
     6721 caaccaccgc aatggtggcc gattttttca gcggtaacgt ttcgagacgg tttttcagca
     6781 acaccaggct ttcgcgcgcc acttcacgcg cttctttacg gtgcaggcgg ctttcggcat
     6841 tggtatccac cgggtcagac tctttcggcc ccaaatggct gtatgggtcg ttaaacaatc
     6901 ccatatcata tttaacgttc agtacatggc gggcagcatc gtccagctct tccatcgtca
     6961 ctttgccgga tttgatcaac ccaggcagat acttcgagta atactcgtcg ctcatactca
     7021 tgttgattcc ggatttcagc gccacgcgca ccgcatcttc cgggtctgcc gccgtgccat
     7081 gtttaatcag ctctttgatt gcaccgtgat cggaaacggt gatgccttta aagccccact
     7141 ggtcgcgcag aacatctttc agcagccagg aatcggaggt ggctggcgtg ccgttcagcg
     7201 agttcagcgc caccatcacc gcgccgctgc ctgcgtccag ccccgctttg tacggcggca
     7261 tataatcatt aaacaggcgc tgcggactca tatcgacggt gttgtactct ttaccgcctt
     7321 ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat caccgagtag cgatctgccg
     7381 ggcttttacc ctgcatcgct tccaccatgg ttttacccat tgttgaggtg agatacgtat
     7441 cttcgccaaa accttcggaa gcacgtcccc agcgcggatc gcgcgagaca tcgaccatcg
     7501 gtgcccaggt catattcagg ccatcatctg ccgcttcata agcagagaca cgtccgaccg
     7561 ttttcactgc atcgaggtta aaagacgagg ccagaccgag gctaatcggg aacaccgtgc
     7621 gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat tttcaggcgg cttaattcca
     7681 tcacctgatc ctgcatggcg cggatatcct gacgggttac ggtgttgaaa atcgccccaa
     7741 cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg gttatccgga ccgacgctga
     7801 ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt cttaagcagt tcggtgacga
     7861 acgcatcccg cgcttcgggc gttaatggat ggttgccgaa taaatcatcc gccagtgctg
     7921 gctgcagggc cagactcacc gcgattccta ctgaacatag ccatttcata tggatttttc
     7981 ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa accgtagttt gccataagca
     8041 tgatggagag agaaaaagaa tgctcagttt attgtctgaa ttttcaaaat attcactcgc
     8101 tgaattgtca tacaaggcgc tattctagtt tgtgatattt tttcgccacc acaaggagtg
     8161 gaaaatgtct tccatgacaa caactgataa taaagccttt ttgaatgaac ttgcccgtct
     8221 ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg gcccgctatc gcaagggctt
     8281 ccgttctggt cagggcgacg cgctggctgt cgttttccct ggctcactac tggaattgtg
     8341 gcgggtgctg aaagcctgtg tcaccgccga caaaattatt ctgatgcagg ctgccaatac
     8401 aggcctgacc gaaggatcga cgccaaacgg taacgactat gatcgcgata tcgtgatcat
     8461 cagcaccctg cgtctcgaca agctgcacgt tcttggcaag ggcgaacagg tgctggccta
     8521 tccgggcacc acgctctatt cgctggaaaa agccctcaaa ccgctgggac gcgaaccgca
     8581 ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc ggcggtattt gtaacaactc
     8641 cggcggctcg ctggtgcaac gtggcccggc gtataccgaa atgtcgttat tcgcgcgtat
     8701 aaatgaagac ggcaaactga cgctggtgaa ccatctgggg attgatctgg gcgaaacgcc
     8761 ggagcagatc cttagcaagc tggatgatga tcgcatcaaa gatgacgatg tgcgtcacga
     8821 tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt cgtgatattg aagccgacac
     8881 gcccgcacgt tataacgccg atcctgatcg gttatttgaa tcttctggtt gcgccgggaa
     8941 gctggcggtc tttgctgtac gtctagatac cttcgaagcg gaaaaaaatc agcaggtgtt
     9001 ttatatcggc accaaccagc cggaagtgct gaccgaaatc cgccgtcata ttctggctaa
     9061 cttcgaaaat ctgccggttg ccggggaata tatacaccgg gatatctacg atattgcgga
     9121 aaaatacggc aaagacacct tcctgatgat tgataagtta ggcaccgaca agatgccgtt
     9181 cttctttaat ctcaagggac gcaccgatgc gatgctggag aaagtgaaat tcttccgtcc
     9241 gcattttact gaccgtgcga tgcaaaaatt cggtcacctg ttccccagcc atttaccgcc
     9301 gcgcatgaaa aactggcgcg ataaatacga gcatcatctg ctgttaaaaa tggcgggcga
     9361 tggcgtgggc gaagccaaat cgtggctggt ggattatttc aaacaggccg aaggcgattt
     9421 ctttgtctgt acgccggagg aaggcagcaa agcgttttta caccgtttcg ccgctgcggg
     9481 cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc gaagacattc tggcgttgga
     9541 tatcgctctg cggcgtaacg acaccgagtg gtatgagcat ttaccgccgg agatcgacag
     9601 ccagctggtg cacaagctct attacggcca ttttatgtgc tatgtcttcc atcaggatta
     9661 catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa cagatgctgg aactgctaca
     9721 gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt catttgtata aagcaccgga
     9781 gacgttgcag aagttttatc gcgagaacga tccgaccaac agtatgaatc cggggatcgg
     9841 taaaaccagt aagcggaaaa actggcagga agtggagtaa aaattacgga tggcatagta
     9901 tcgccatccg tcattcactt aatcgttctg tgccgtctgc cccgccgccg ccatttgggc
     9961 ggctttttgt tttttatagc tcaacgctgc tgctggaaca ggcatcactt taccagtttc
    10021 aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa acgcatccat
    10081 cactaccagc gctaccggtt tattattgat aaccgtacgc atcaccagac aatggcccgc
    10141 cgcattggta aaaccggttt tggttaactg aatattccag ttatcgcgat acaccagatg
    10201 gttagtattg cggaacggca gcgtatacgt cgggttagag aaggttgcca tatcttcccg
    10261 ggtagtactt aactgcccga tcaacggata ttgtttgctg gcaatgagca gtttggttaa
    10321 gtcacgggca gttgaaacgt tatgcaccga caatccggta ggttcaacaa agcgcgtgtt
    10381 gttcattccg agcgatttcg ctttcgcatt cattgcctta ataaaggctt tgtaaccacc
    10441 gggataatgg tgtgcaaggc ttgccgccgc gcggttttct gaagacatca gcgccagcaa
    10501 cagcatatct ttacggctga tttcgctatt cagtcgtacg cgcgaataga cccctttcat
    10561 ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc gtgcatccag
    10621 cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca gatccgggtg
    10681 gttcgagtag atcactttgt tggtattcag atcaacaatc atcgcgctac cggaggcaat
    10741 ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg gtgccaaagg
    10801 cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca tgatgagcat
    10861 tcagatagtg gttcacgcgc acaggttgcg caccgccgga gtaaggattt actgaggcta
    10921 gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag gagaatcgtg aggaaatgct
    10981 gcattgctga catttacgcc agcaatgcaa cgtcaaagaa ctttctagaa caaacgataa
    11041 ccgtagcccc atagtataac ggttagggcg agcagcactt ccagtaccag cacgccaatc
    11101 gccagcgtcg aactagagaa gctaaggcct tcctctttgt tgatattcag gaagctcgga
    11161 ataccaaggt aaagcaggta gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac
    11221 aaccagacca gtggataaag cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag
    11281 cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg gataattacg cgccatccac
    11341 cagatgaccc gccccattac cgcaacccca gccagcataa cgccataaaa caggacagcc
    11401 agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa attccagcca
    11461 atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg
    11521 tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg
    11581 ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga gttcatgaac
    11641 ctgacagttc aagtataagt cagcttgtga ttattttttg ttcgctcatg taatttgcac
    11701 cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag ggtgtatgct tacaggcaac
    11761 caaagggaga cagactggcc tatggatctc aatacactca tctcacaata tggttatgcc
    11821 gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga ctttgctggg aggcgttgcg
    11881 gcacatcagg ggctattaaa gttcccgctg gtggtacttt ctgtggcgct tggcggcatg
    11941 attggcgacc aggtgcttta tctgtgcggg cggcggtttg gcggcaagct gttacgccgt
    12001 ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg ccatccgtat
    12061 ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg tgattggccc gacgctgatt
    12121 ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg cgcatttgcc
    12181 tgggcgttga tttttaccac tattggttac gctggtggtc aggtgattgc gccgtggttg
    12241 cacaatctcg accagcattt gaagcactgg gtctggttga ttctggtcgt ggttctggtg
    12301 gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa agccggatca tcaggcgtaa
    12361 aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca tccggtgcac catgcctgat
    12421 gcgacgcttg tgcgtcttat catgcctacc aaaattttac ccattgaaat aaccacgcac
    12481 ctcactgcgc tattctgggt tgaactgcgg attcgccaac ataaagccgc catccactat
    12541 caacgactgc ccggtggtgt aatttgcgcc ctctgagcac agccacgcta ccaggctggc
    12601 aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa ggctccgcgt cgggcttcac
    12661 gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc ccaggcgcga ctgcgttcac
    12721 caaaatctta tgcctgacca gctccagcgc catcgttttg gttaacccgc cgagcgcatg
    12781 tttagcggct gtgtaagcgc tggcatccgg cagcggcgta tgttcatgta ccgacgtaat
    12841 gttgatgata cgaccgccct gcccttgttt caccatctgg cgggccgcaa tttgtgagca
    12901 taaaaatgca ccatcgacat caacggtaaa aatcttgcgc cactcatcga aagccatatc
    12961 aagaaacggc gctttggtca ttgcgcccgc attattcacc agcacatcaa tacgccccag
    13021 ccgttgaatg agtttctcca gcgcctgcgc accttctggc agtttaccga gctccagctg
    13081 cacgatctcc gcacgtacgc cgtggctaac tacctcacgc gcggtatctt ttgccccttc
    13141 ttcatctgag tgccaggtaa tgccaatatc aaacccctgc tgcgccagta ataacgcgca
    13201 ctctttgccg atccccgaat cggaggcggt aataatcgca acctgtgcca tcaagttctc
    13261 cacttaacgc tgaataaacg ttaagtatag aaggcgcata tcatcagcgt ttgtaacccc
    13321 cgcccaacgc accagtgagt tgaatagaag catccagcca ctgcccttgc aataacaggc
    13381 cattggcccg ctcacgcagc gcggggattc tggcttcgct gacgcgggaa ccaacaatga
    13441 tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg caaggcatcg cgctcaattt
    13501 gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg actggctgcc cgcgccacgt
    13561 cattcaccgc ttcaaccacc gctttgttgt agctggcgat agacaagttg ctttcggctt
    13621 ttgcgatatt gagattggcg ttaagacgac cactatcgaa aatgggtagc gtcaggcctg
    13681 ccgtaacgcc catttgctgc gcggaatgac ggaacagatc gcttaagtgc aacgcatcct
    13741 gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc cgcttttgcc gcatcaatgg
    13801 tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa atctgcccgc cgggccagta
    13861 aggagtaccc cagttcatca ggaagctggc ttgccacttt cggcaacgcg accggtttaa
    13921 gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa ccgtgcctca ataattttca
    13981 ttttccccgc gacatcgttg agctgctgcc gggttttgct ggcattaata tcggtttcca
    14041 caccttcaac tgaagaagta atcccgttct gatatagctg gcgatcggtc gcgataatgg
    14101 tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa cgccgcctgg gtttgccact
    14161 cccagtacag gcgggctacg ctgccagcca gcaattggcg ggtttgctcg cgttccgccg
    14221 cccgtgcttt aaccgtaccc aggcgggcag taacctccgc ccgattcttt ccccagatat
    14281 cgagatgcca gcccgccgtt aagccaaaag taccgttggt gtaccacggg ccggtcgtac
    14341 ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc ttctgccgac attttttgcc
    14401 gctccatatc cgccgaaaag tcgatctgcg gaccatcctg agtggcaact gccttcgcct
    14461 gggcttcagc tagctgaatg cgctgttcag ccacctgcat atccggtgcg ttctgtagtg
    14521 cattgttaat taatgaagtg agctgattat cgtgatactc cagccaccat tggctgtctg
    14581 gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac ttgtgcagcg ggcgtttgct
    14641 ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc ggccagcatc agtaacagcg
    14701 gaaaacaggc gatggctgga taaaaggaat cacgattcat gggggaataa tcaggtaaga
    14761 aacggtgcgc ggagattacc gtgtgttgcg atatattttt tagtttcgcg tggcaataca
    14821 tcagtagcaa taaaacgaca tacccagaaa aatatagact aagtaaatga tatcttccga
    14881 tttatcttaa tcgtttatgg ataacggcaa agggcttcgt tttttcctat acttattcag
    14941 cactcacaaa taaaggaacg ccaatgaaaa ttatactctg ggctgtattg attattttcc
    15001 tgattgggct actggtggtg actggcgtat ttaagatgat attttaaaat taattaatgt
    15061 catcaggtcc gaaaataacg agaatatttc agtctctcat cctgttgcgc tcctgtcatg
    15121 tgcattgctt catataatca ctggcgcaag gagcgcgcag ggggcggcca atcgccgccg
    15181 ccccctgcac ccccgggctc tggcgaacaa aatcgccgct gcgcggtgcc ctcggcttat
    15241 cccttacggc taccgggtcg ggcgcgaggt aacatccctg taaaacgcgc cctcagccca
    15301 cctccatgtg ggctgccccg gccttcaggg aacgcctcgg caattttgac gccaccaaac
    15361 aaccgtgcgg cctattgata aagagctaac acattgtcaa aaaacatcac tatggttttt
    15421 tagagtttct cgatatcaat tgcctgaata gcccttgcaa tatcagggga attattcaac
    15481 acccgaacat gctgaaataa ttccgttgct tcatcgtatt ctttacgcaa ataactcaac
    15541 cactgtttaa tccgcgcaac gtgatataac ccggtatcgc cctgcttttc cagacgggta
    15601 tatttttgca gcaaagcaac cacctccggc cacggcattc gcggttcgtt atattttact
    15661 acccggctca ggttgggaat attgagcgcc ccgcgaccaa tcatcactgc gtcgcagccg
    15721 ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga tttcgccgtt ggcaatcacc
    15781 ggaatattca gccgctggcg aatctcgcca atcgcctgcc agtcaatatg ctccgcgcgg
    15841 taaccctgct cttttgtccg tccatgcacc accagctccg tagcaccagc ctgttgaacc
    15901 gcatcggcga tttcaaattt cttctcaccg ctgtcccagc ccagacgcac tttcacgctg
    15961 acgggcaaat gcgccggtac agcttcacgc atcgcttttg caccctggta gatgagttca
    16021 ggatctttga gtaacgtcgc cccgccgccg ctgccgttaa ccgttttcga cgggcagccg
    16081 caattgagat ccacgcccca ggaacctaat tccaccgcac gggcggcgtt ctctgccagc
    16141 cattgtggga actgacctaa caactgaacg cgcaccagcg taccggatgg tgtccggctg
    16201 gcgttttgta gctcagggca aatgcgatga aagactttta ccggcagcag ttgatccacc
    16261 acgcggacaa actcggtgat gcacagatcg tagtcgttaa cttcggtcag caattctcgc
    16321 accagagagt caagcacacc ctccatcgga gccagtaaca cacgcatatc ttcacccgga
    16381 aaaaaatgag gcgctatgtt agcgcctatg gtcaacggat taaaggtctg caaagaggga
    16441 ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc ctgagttcgt tgcgcattgc
    16501 gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa tgtgatcggt agcacgtttt
    16561 acgaattaat tgtatgatga atccatctca tctggggtgt tgattatgag taagacactg
    16621 aacattatct ggcaatattt acgcgctttc gtcctgattt atgcctgcct gtatgcaggc
    16681 attttcattg cttccctgct accggtaacc attccgggca gcatcatcgg gatgctgatc
    16741 ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat gggtcaatcc ggggtgctac
    16801 gtactgattc gctatatggc gctattgttt gtgccgattg gcgtaggcgt catgcaatat
    16861 tttgatttgc tccgcgcaca gtttggcccg gtagtggttt cctgtgcagt cagtacgctg
    16921 gtggtttttc tggtggtgag ctggagttca caactggtac acggtgaacg taaagtcgta
    16981 ggtcagaaag gatcagaaga atgatggcga atatctggtg gtcattaccg ctgacgttga
    17041 ttgtcttttt tgccgcccgc aaactggcgg cacggtataa gtttcccctg cttaacccgt
    17101 tgctggtagc aatggtggtg atcattcctt ttttaatgct gaccggcatc tcttacgaca
    17161 gctactttaa aggtagcgaa gtgctaaacg atctgctgca accggcggtc gtcgcgctgg
    17221 cctatccttt atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg atcatcacca
    17281 tctgttttat cggcagcgtg gttgcgatgg tgacggggac ttccgtggca ttgttgatgg
    17341 gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg cctattgcaa
    17401 tggcggttgg cggcagtatt ggcggtattc cggcaatcag cgcagtttgc gtgattttcg
    17461 tcggcatcct cggcgcggta tttggccata cattgcttaa tgcgatgcgt attcgtacca
    17521 aagctgcgcg cggtctggca atggggacag cctcgcacgc cctcggtacg gcgcgctgcg
    17581 ccgagctgga ttatcaggaa ggtgcattta gttcgctggc gctggtgtta tgcgggataa
    17641 ttacttcgct gatcgcaccg ttccttttcc cgattattct ggcagtaatg ggctaaaatt
    17701 tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa tgagattcag
    17761 atcacatata aagccataac gggttcgtaa actgttatcc cattacatga ttatgaggca
    17821 acgccatgca tccacgtttt caaaccgctt ttgcccaact tgcggataac ttgcaatctg
    17881 cactggaacc tattctggca gacaagtact tccccgcttt gttgaccggg gagcaagtct
    17941 catcgctgaa gagcgcaacg gggctggacg aagacgcgct ggcattcgca ctacttccac
    18001 tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca attgcgcgcg
    18061 gtgtgagcgg aacctggtat ttcggtgcca atatggaatt tattggtgca acaatgcagc
    18121 aaaccgttca tgccgaacaa agcgcgatca gccacgcctg gttgagtggt gaaaaagcgc
    18181 ttgcagccat caccgttaac tatacgcctt gtggtcactg ccgtcagttt atgaatgaac
    18241 tgaacagcgg tctggatctg cgtattcatc tgccgggccg cgaggcacac gcgctgcgtg
    18301 actatctgcc ggatgccttt gggccgaaag atctggagat taaaacgctg ctgatggacg
    18361 aacaggatca cggctatgcg ctgacgggtg atgcgctttc tcaggcagcg attgcggcgg
    18421 caaaccgttc gcacatgcct tacagtaagt cgccaagcgg tgtcgcgctg gaatgtaaag
    18481 acggtcgtat tttcagcggc agctacgctg aaaacgccgc attcaacccg actctgccac
    18541 cgttgcaggg ggcgttaatt ctgttgaatc tcaagggtta tgattacccg gatatccagc
    18601 gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca gtgggatgcc acctccgcaa
    18661 cgctgaaagc tctcggctgt cacagtatcg accgcgtgct tctcgcttaa gcctggtgcc
    18721 ggatgcggcg tgaacgcctt gtccggcttg ccagccctct cctggtgtcg aaattcccgg
    18781 caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa gtagcctgat ggaaattttc
    18841 cttagatcga gtctcctgca tgttaaagcg cgtgttcctc agcctgttag tcctgatcgg
    18901 cttgctgctg ttgactgtgc tcggcctcga tcgctggatg agctggaaaa ccgcgcctta
    18961 tatctacgac gaattgcagg atctccccta ccgccaggtc ggcgtggtgc tcggcacagc
    19021 aaaatattat cgtactggcg taattaatca gtattatcgc taccgcattc aaggagcgat
    19081 taatgcctat aacagcggta aggtaaatta tctattactg agcggcgata acgcattgca
    19141 aagttataat gagccgatga ccatgcgcaa agatttaatc gctgctggtg tcgacccatc
    19201 agatattgtt ctcgattacg caggctttcg tacgctggat tccatcgtgc gtacacgcaa
    19261 agtgttcgat actaatgatt tcattattat cacccaacgt ttccactgtg agcgggcatt
    19321 atttattgcg ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt caccgaaaga
    19381 tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc ggtgcgctgg ctgaccttta
    19441 tatttttaga cgtgaaccgc gttttttagg gccgctggtc cctattccgg ctatgcacca
    19501 ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac ttgaattaca
    19561 aaagaaacaa ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc cgtttttttc
    19621 cttatcccga tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg cgcgattgat
    19681 aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa tcccggactc
    19741 tttttcgctt atatggtggc atatatcggc ttcggaattt tatctatcgg catgattgtt
    19801 tatcttattt tctatcgtta atattttctc ttttctgtta attattgaga ataattatta
    19861 cttcactcaa taagctgcgg atataacatt ctgaaccgca gctatttgtg aatcctttca
    19921 cagtttaaat ttccccacac gcttagcctt aatatcagca cgcaatattt atttagcgtt
    19981 cgccttaatt actcatagca ttaaggaaga tcacatgccg caacaaaatt atctggatga
    20041 actcactccg gcttttacgc ctttactggc gattaaagaa gcctctcgct gtttattatg
    20101 tcatgacgct ccctgtagtc aggcttgccc ggcacagacc gatccgggga aatttattcg
    20161 ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa ataatgccct
    20221 cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta tgccaaagcg gttgtacccg
    20281 tgccggtgtt gatgctccca ttgatatcgg ccgcttacag cgttttgtta ctgattttga
    20341 acaacaaacc gggatggaaa tttatcagcc cggtactaaa acgctcggca aagtcgcgat
    20401 tattggcgca ggtcctgccg gattgcaggc cagtgtgaca ctgacaaacc agggttatga
    20461 cgtcacgatc tatgagaaag aagcacaacc cggtggctga ttgcgtaacg gcattccgca
    20521 attccggtta ccgcaatcag tactggatgc agagatcgcc cgtattgaaa aaatgggcgt
    20581 gaccattaag tgcaacaacg aagtcggtaa cacactcacc cttgagcagc tgaaagcaga
    20641 aaaccgcgcg gtactggtca ccgtggggtt atcaagcggt tccgggctac cgctgtttga
    20701 gcatagtgac gttgagattg ccgtcgactt cttgcaacgt gcacgacagg cgcaaggcga
    20761 tatcagcatt ccacaaagcg cattaattat cggcggcggt gatgtcgcga tggacgtagc
    20821 cagcacgctg aaagttctcg gctgtcaggc ggtaacttgc gtagcgcgtg aagagttaga
    20881 tgagtttccg gcaagcgaaa aagagtttac cagcgcccgg gagctgggcg tttcgatcat
    20941 cgatggattc acgccagtag ccgtggaagg caataaagtc acgtttaagc atgtacggtt
    21001 atcgggcgaa ctgacgatgg cggcagataa aattattctc gccgtcggtc agcatgccag
    21061 actagatgcc tttgcggagt tagagccgca gcgtaacacc atcaaaacac aaaattacca
    21121 gacccgcgac ccgcaagtct ttgctgctgg cgatattgtt gagggagaca aaaccgtggt
    21181 ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt catcactatt tagagggagc
    21241 ttgctcatgt taacgaaaga tctttcgatt actttttgcg gcgtgaagtt tcccaacccg
    21301 ttctgcctct cttcttcgcc ggtaggcaac tgctatgaga tgtgtgccaa agcctacgac
    21361 acaggttggg gcggtgtggt gtttaaaacg atcggctttt ttatcgccaa cgaagtctcg
    21421 ccgcgttttg atcatctggt gaaagaagat accggtttta tcggcttcaa aaatatggag
    21481 cagattgccg agcatccgct ggaggagaat ctggccgccc tgcgtcggct gaaagaagat
    21541 tacccggaca aagtattgat cgcttcgatc atgggggaaa atgagcagca atgggaggag
    21601 ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg agtgtaactt ctcctgtccg
    21661 caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct ggtagaaaaa
    21721 tattgtcggg cagtgaaacg gggttccacg ctgccaatgc tggcgaagat gacgccgaat
    21781 atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga tggcattgcg
    21841 gcgattaaca ccgttaaatc catcaccaat atcgatctta atcagaaaat cggtatgccg
    21901 atcgttaacg gaaaatcaag tatttccgga tattccggta aagcggtaaa accgatcgcc
    21961 ctgcgcttca ttcagcaaat gcgcacccat ccagaactgc gcgatttccc aatcagcggt
    22021 atcggcggca ttgaaacctg ggaggatgcg gcggagtttt tattgctcgg cgcagcaacg
    22081 ttacaggtca ccaccggcat tatgcagtac ggctatcgca tcgtagaaga tatggcgagc
    22141 gggttatcgc attatctcgc cgatcaggga tttgattcgt tgcaggagat ggtaggtctg
    22201 gcgaataaca atattgtccc ggcggaagat ttagaccgca gttatattgt ctatccccgt
    22261 atcaatcttg acaaatgtgt tggctgtgga cgctgttata tttcctgtta cgacggcggt
    22321 caccaggcga tggaatggag cgagaaaacc cgcacaccgc attgtaatac cgagaaatgt
    22381 gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct cggggaagtg
    22441 aagtttaaga aaggcgagaa agaacacccg gtaacgttgt agaacccgcg tcacatccgg
    22501 catttggttc ctgttgccgg atgcggcgtg aacgccttat ccggcctaca aaaccacaca
    22561 gatcatgatg ttatgtgcgt ttatgaccga atgcggacca cattcacatc atttcttacg
    22621 cgcgtatttc agtgaatcca gcgctacggc gaagataata atcgcccctt tgatgatgta
    22681 ctgccagtat gggtttacgc cgatatacgt caggccatag ttgatgacgg taaaaataat
    22741 caccccggtc accacgccaa tcaccgtccc cacaccgccg ctgaacgata caccgcctac
    22801 cacgcacgcc gcgatagcat ccagctcata cataaagccg aggttgttgg tggcagagcc
    22861 gatacgtccg gcttctaaca tcccgccaaa ggcatagaac acgccagaca acgcgtagat
    22921 catcagcagg ttcaggccga cgttgacacc agatactttt gccgcttccg ggttaccgcc
    22981 aatggcaaaa atgttcttac cgaagcgggt tttgttccac aacacccaga cgaacgccac
    23041 cgcaatcaac gcgtagaagg tgatgtaaga gagacggaaa ctccccagcg cgacaaagcc
    23101 ctgagcaaag gtagagaagc cactgtcaaa accagaaatt ggcgacgccc cgacaaagtc
    23161 gtaatagagc gagttgatgc catagacgat gatcatcgtg ccgagcgtgg taatgaacgg
    23221 cgtcacgttg agataagcga taatcagacc gttgatcaaa ccgatcaccg caccaatggc
    23281 acagacaatc agaataacca gcgcaatcgg catcgtcgcc atttccggga acactttgtt
    23341 ggcgttatcc atggactgca ataatgtcgc agccaccact gccgccagcc ctacctgacg
    23401 accagcagaa agatcggtcc cctgggtgac aattaaccct gccacaccga gcgcgataat
    23461 aatacgcacc gatgactggg tgagaatatt acttaagttc aacagactta aaaatgttgg
    23521 gtcctggaaa ataataatcg ccagcaaaac taaaagaacg acgtaaatac cgccctcttt
    23581 caggtaagta agaaaacttt tcttatttaa cgcactcatg ggaagcccct aatcttaaag
    23641 gtgcaaagac gcaagacgca gaatttcgtt ttgcgttgtt gtttttgtat cgacaattcc
    23701 ggaaacgaga ccattgctca tgaccagaat acggtctgtt atccctaaca actcaggcat
    23761 ttcagaggag ataataataa tccccttgcc tttcttcgcc agttcggcaa ttaactgata
    23821 aatttcaaac ttcgccccga catcaatacc gcgcgtcggt tcatcgagca ttaatatttc
    23881 tggttgcgtt agtagccagc gaccaataat caccttttgc tgattaccac cggagagcga
    23941 accaatttgc gtccgatgac ccggcgtttt tacccgcatc gaatcaatca cccactgggt
    24001 atcgcttttc atccgcgagt tatccagtaa accaacttta tttttgtagt tgcgaatatt
    24061 ggaaattaac gagttaaaac caatatccag ataggcataa attcccgttg agcggcgctc
    24121 ctcagttacc agtgcaaatc cgtggtttat ggcttcgttg gcattatggt tattgatctg
    24181 tttgccgtgc aacgtaatgg tgccagccga tttctcgcga ataccaaata acgtctcaac
    24241 aatatcggta cgtttcgccc ccaccagccc ggcaataccg aggatctccc ctttatgcag
    24301 atcaaacgag acatcgcgaa tcgacggctg gcgcagtgac gttaggttac gcacctcgag
    24361 gatgacttcg cccggcttgt tttctttgtc aggaaaacgc tgattaagag aacgcccaac
    24421 catcatggcg atgatcttgt ccatcgtcag tcctgccagc ggttcggtgg cgatccactg
    24481 accgtcgcgc aatacggtaa cttcatcaca taactggaag atttcttcca ttttatgcga
    24541 gatataaaca ataccgcagc cgcgctcttt taatttacga ataatagtga acagatgatt
    24601 gacctctttt tcggttaacg aagaagtcgg ttcatccata atcacaattt tcgcgttata
    24661 ggaaaacgct ttggcgattt cgatcatctg catttgcgaa acggataatg tgccgacgcg
    24721 cgcacgcgga tcgatatcaa tatccagttc atcaaaaatt gctttggttt cgcggtacat
    24781 tttgtcctga tcgacaaaca tgcctttggt gggatatcgc cccagccaca tgttatccat
    24841 caccgaacgt tgtaatacca ggtttaactc ctggtgtacc atcgaaatac cattttccag
    24901 tgcctctttg gcggaatgga aatcgatctc tttaccctgg aataaaatgg tgccggagtc
    24961 tttttgataa ataccaaaca ggcattttaa taatgtcgat tttcctgcac cgttttcccc
    25021 cattaatgca tggatagaat gtggccggac ttttaaatta acgttatcaa gtgccttaac
    25081 accaggaaag gacttgttga taccgctcat ttccaacaag tattccccgg aagacggagt
    25141 cgttgagctg accatataat tttaccttgt tggccataca ataagggcgc agtaaacgac
    25201 tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg ttgtctttat ctacgccaac
    25261 ataaggtacg cggaccactt tgttgtcgat tttccagttg gtgccatcag ccgcaccttt
    25321 gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc gcctggttgt tagcatcgtt
    25381 cagtactgtg cccgccagtg caccggattt caccagcgcc agcgcttctg gcagcgcatc
    25441 gacgccaaac accggaatgc tggacttgtt gtgtgctttc agtgcttcta ccgcgcccat
    25501 tgccatcgca tcgttgttgg cgataaccac ttcgattttg ttggcgttcg ggccagacag
    25561 ccaggcgtcc atcttatctt tcgcctgagc ggtgtcccac attgcggtat ctaactgtaa
    25621 ctgttcagtt ttgatgcctt tatcgttcaa ttctttaatc acgtaagtgg tacgtgcttc
    25681 tgcatccgga tggcccggtt cacctttcag cagtacgaac tgaatctgac cgtctttgtt
    25741 cagatcccaa ccctgattcg ccgcccagtg tttagcaatc aaatcgccct gaataatgcc
    25801 ggactctttg gagtcagtgc caacgtagta ggctttgtcg tagctatcca gcgccttacg
    25861 agacggttct ttgttgaaga aaaccaccgg cacgttttgc ccacgcgctt tctcaatcac
    25921 cgtacccgca gctgccgggt caaccaggtt gattgccagt gccttcaccc ctttcgccag
    25981 caatacgtcg atctgatcgt tctgcttgga ctggtcattc tgagaatcat tcatcagcag
    26041 ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc ttgcgcacta cagacataaa
    26101 gttatcgtcg tacttataga ttgttacacc aatgcgagta tcagcagcgt gtgcagcggc
    26161 accgaataac atgctggcca tcacagcaga cagggttaac accttcttat tcatggtatc
    26221 tccggttttt cttatgcagg gtagtgcttg agataaatgc tcggcggggc agtagagtta
    26281 atgaagtgtt actgaacgcc gaagctcact tttttaaatt cgttcttcca tgcccggtaa
    26341 cgctccagaa aacggcttta attgttgtta tgacgctatt acctcggtga aagtgattat
    26401 tcactgttac atacgggtta caacgttaaa acggtgcaat catagctatc acattgttaa
    26461 gatactgtga aatcactcac agattgaaag cggttacatc gcctgatttg ttgagttagt
    26521 gatcgccgcc gcattctggc gcgttgcgac agaatggcga cgcactaacg tcggcataaa
    26581 acagtggctg gcacgaggat caatattgcc tgctgccccc tgcaaggcca gttcggtggc
    26641 taatttagcc attgaagcaa tgggataacg cacggtcgtt aattgcgggt cggtgtaacg
    26701 ggcgatggga atatcatcga aaccgatgat tgagagatgt aacggaatcg caatgccatt
    26761 atcttttaat gctgtcagtg cgccagcggc catattgtcg ttataagcaa atatagcggt
    26821 aagttgtaga ttgcgcccca gcagttcaac catcgccgcc tcaccgcccg gcatgtccgg
    26881 cgtaccagtg ccaatccagc tttccggcgg aataatatcc tgctctttca acgcactcat
    26941 ccagcctgct ttacgcatgg cgtcatcttc aatgccgtga ctggaagaaa gataaccaat
    27001 acgttgatga ccgttattca gcaacatgcg cgtcgccatt cgggcaccgc tgagattatc
    27061 caggcaaacg caacgatggg cgtaccccgg cacaacgcgg ttgattaaca ccataccggg
    27121 aatgttatcc ataaattgcg ccagttcatc gtcactcaat gcttttgagt gaacaatcaa
    27181 cgcattacaa cgctggcgaa ttaacacctc aatggcgtga cgctcttttt ccgcttcatg
    27241 atagctattg ccgattagca cgtatttctg atgctgctga gcgactagat ccaccgcttt
    27301 taccagcgcg ccgaaaaacg catcagaaac gtccatcacc accacgccaa tggtgtcgct
    27361 aacctgagtt gccagcgcct gggcattggc gtttggccga taatccagct cactcacggc
    27421 tttcattact gcttcacgcg tgtcggcact gaccagcgtg ctgttattga gcacccggga
    27481 aaccgttgcc acagagacgc cagcctaacg cgctacatca cgaatggtga tcatattcac
    27541 tacctgttat gagattgcag taaatgactg cttgctggcg gctattttgt cagcactgaa
    27601 tacaggactt cgtgaatcga gtcacagcaa tggaaacggt tacagccgtt ttgttaacga
    27661 atgtgatcca gatcgttatc ttgatgtttt agatattgcc gttccggcag cacgcaaagt
    27721 taactgacgc cagagccatt ccaccggccc ctggcggaag taacgcagcc agataacaga
    27781 gaagaggata ttcgccagcc ataccggaat aacaaacgcc agcagctcca ggcggtcaaa
    27841 atgcataaac aaaccgaggt ggtaaaaaag catggtacaa atcagcgttt gcaatagata
    27901 gttggtcagc gccatccgtc cgacgcaggc gatcgcaagc accagcttaa agcggctcaa
    27961 ttgcggccag aagccataaa acagcgacgc atagccaatc gcctgaaacg gcgcactcag
    28021 ttcccgcggc atttgaagta agaaggcaca ccagcgatac gcccagtcca gctgccattg
    28081 cagggcgatg gcaggaaggt taatggtcac cccaatcgcc accagcacaa aaccagtacg
    28141 acgatagtga cgtaagctga actgcccttt cagccagccg ctgcgcatca gcgccgcacc
    28201 aatgagcatc atccctgcca gttgccagcc atactgtgcg cccagtgcca gtaaactgtt
    28261 gccaacacca tcggcacgat tactgatcgc ttcaacgccg ccgtgaagct tccagtattt
    28321 ttcatacaaa atagccgatg catccggcgt ccaggtgcgg ctggtctggc tgtcggaaat
    28381 cagccccaat aacagcaaaa cgccaagccc caccagataa agcatgacgc ctgtattaaa
    28441 caggcttttt accgatggcg catcgcgcac cagccgccag cagattaagc ccaccagccc
    28501 gtaagccagc agaatatcgc cgtcccagaa caataaaccg tgaataaaac ccagcaagac
    28561 taacagcgtt aaccgcgact ggatccagcg tctgccacgg ggcagcaaca tttgcaggcc
    28621 cgcaccaaac agcagcgcaa aaagcgtgag gaatttcacc tggccgatga gatcgagaaa
    28681 tgcccaggtc catgcatcct gcggcgtaat agcgccgtac caggcgggat tgagataagc
    28741 cgcctttggt agcccaaagg cgctgatgtt taatagcagg atccccagaa tggcgacgcc
    28801 gcgaacaaaa tcgagcgtta cgttgcgctc catggttcct gccttttaat cagttgtgat
    28861 gacgcacagc gcgcagaaac tcgtggcgcg tattctgact ggatttgaac aatccaccaa
    28921 gagaggtcgt tgttgtggca ctggttgcat cgcggatgcc acgcgctttc acgcagtaat
    28981 gcaccgcatc aatggagaca gccacgttat tggtgcccag cagcgtttgt agcgcaataa
    29041 gaatttgctg cgtcagacgt tcctgcacct gcggacgctg ggcaaagaac tgcacgatac
    29101 ggttaatttt tgacagaccg atcaccgaat ctttcgggat ataagccacc gtcgctttgc
    29161 catcgatgat aacaaaatgg tgttcacagg tactggtcag agtgatatcg cgcacggtga
    29221 ccatttcatc gaccttcatt ttgttttcaa tgagggtgat tttcgggaag ttggcgtaat
    29281 ccagaccgga gaaaatttca tcgacataca ttttagcgat gcgatgcggc gtttccatca
    29341 aactgtcatc agccaggtcg agattcagca gctgcatgat ttcggtcata tgaccagcaa
    29401 taaggctttt gcgcgtttcg ttatccattt catgcacggg cgggcgcagc ggtgtttcca
    29461 gtcctcgcgc aactaacgct tcatgaacca gggccgcttc tttactgagt gatggcattt
    29521 atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg gggcaaagtt attaagctga
    29581 tttacagcct gattattgtg cgtgaggcgg cgcacataat ccagtattca cagcgataat
    29641 tattgtaatt gccgctgact ttcatcagca gatgttaaaa catcgttatg caaatacgga
    29701 agtgaaagtt actcacagca cattgaataa acggtatgat gaagaaattg caaacaacac
    29761 aacaaggagc cacgcatgga aatgctcgaa gagcaccgct gttttgaagg ctggcagcaa
    29821 cgctggcgac acgactccag taccttaaac tgcccgatga cgttcagtat ctttctccca
    29881 ccccctcgtg atcacacccc gccaccagtg ctgtactggc tttccggatt aacctgcaat
    29941 gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg cggcagaact ggggattgta
    30001 ctggtgatgc cagacaccag cccgcgcggc gaaaaggttg ccaacgacga tggctacgat
    30061 ttaggccagg gcgcaggctt ttatcttaat gccacgcaac cgccgtgggc gacgcattac
    30121 cggatgtatg attatctgcg cgatgaatta ccggcgctgg ttcagtcgca atttaatgtc
    30181 agcgaccgct gcgccattag cggtcactca atgggtggtc acggtgcgct gattatggcg
    30241 ctgaaaaatc cgggtaaata caccagcgtt tcggcctttg cgccaattgt gaatccgtgc
    30301 agcgtcccgt ggggaatcaa agcgtttagc acctatttag gtgaggacaa aaatgcatgg
    30361 ctggaatggg atagttgcgc gctgatgtat gccagtaacg cgcaggatgc gatcccgacg
    30421 cttatcgatc agggcgataa tgatcagttt cttgccgacc agttgcaacc tgcggtattg
    30481 gcagaagccg cgcgccagaa agcgtggccg atgacgctgc gtattcagcc gggatatgat
    30541 cacagttact acttcatcgc ctcttttata gaggatcacc tgcgcttcca tgcgcagtat
    30601 ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc tcatcagaag cgataatcca
    30661 ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta gtcgtcacga ctgagatcct
    30721 tgtcgccaag gttcagcacg cctgcgcgca gtttgacgtc tttagtcacc tgccaggccg
    30781 cgccggtatt ccagatggta taaccgcccg gtgttttagc cgtcgcgctg tcggcgcgtt
    30841 tctgcccggt atagtgacca gaaacataga atgaccagtc ttccagcgcc agcggtttcc
    30901 agtccagcgt accgttagca gtatggaacg gcagatcgga tagcggtttg ttttcgccgt
    30961 tgctgacatc acgaccatcg ttgtaggtgt agttgatcga cagtttccat tcatcgttga
    31021 acggaatttt cagttcggtt tccacgccct gaatacgagc tttgttaacg ttgtagtagc
    31081 taaataccgg tatgcgccgt ccgttagcgc ccgtctcaaa accaacaaag ttttggtagc
    31141 ccggtgcagc gttgacgtca gacgtacggc tgatgctgat acgatctttc acatcgttac
    31201 ggaaaacggt aacgctggat tcaacccctt ccagccagcc ttcttcaccc atgtagtaaa
    31261 gccccagctc ccaactttcg ctggtttctg gtttcagatc cgggctaccc acaatcttac
    31321 atgcgccacg gcaggaattg ctcgtccagt cagggctaag ttgcaacaga gaaggtgctt
    31381 taaatgccgt cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta taaaccaggt
    31441 aggcacgcgg actccagtgt tcaccgtagg tttcgtgatc gtccatacgc acgccggtcg
    31501 tcagcgccag cggctcgaag atccgccatt catcttccac aaacagcgcg tactggctgg
    31561 cagacgtttt ggagctggtt cccccggtca ggttcaccgc atcgctaagt ttgtcgtgac
    31621 gccattcacc gccaaccgtg agaaactgat taatcgccgt cagcggcaac gtgtatttgc
    31681 cgtcgaccgt attgctttcg gaagttatcg ggctgctgtt gccagggttt ttgttctcga
    31741 ctttctcacc gtagtatttc agttcgctgg tgccgtaatc ccaacgccca ttatggctga
    31801 cggagtagtt ctggcgttcc aggcggtttt tgtccagcga gtcggaatca cgatcctgac
    31861 ggtcgaaacc gtatccggca gtaaaatcgt gattttgatt cggtgtccag gcaaattcga
    31921 cattgccgtc gcggctggag aatccttcaa tacgcggcgt ttctccggta tcggtggtcg
    31981 ttgagttttg cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct ttcattccca
    32041 gcacaccatc aattaatggt ccactggtaa agaactgacc gttataggta tcaccgcgat
    32101 cgcgatgttc ctgaatggtg gtatcgacgg taacggtacc cgaccatttc tgaccgattt
    32161 ttttggtgat gatattcact acaccgccga gcgcatcgga accgtacagc gacgacatcg
    32221 ggccacggac cacttcaata cgttcgatgg aatcgaccgg gatccagttc agatcgaaat
    32281 cattgtggcg gaagacggca ttgcgggagt tcacgcgttt accgtcgacg agaatcaggg
    32341 tatagctgct gtccagacca cgaatactaa cgcccttacg gttatcccct tcgttcgtca
    32401 gttgtacgcc aggcacttct ttgaggacat ccttcagatt ctgtaccggt tttcgctgca
    32461 gatcttcctg ggtaatgacg ctgatactgg cgggtgcatc tttaaggttt tgttcaacgg
    32521 aagatgcagt gacaaccatc gtttcgccat cgtcatcgac cgctaacaca ggccatacac
    32581 aagaaatagc ggacaaacac agcccgaccc gtacgaaagg gttcaaccta aacattccat
    32641 atctccatga ggtaactacg aaaataaaat gggtatcgct cacatcttct ttacgtcccc
    32701 ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag gtgatcatcc cagaaagccg
    32761 gaccagcctc tgatttgtca gccttttttg attgtagcgt aacgataatg caaacgataa
    32821 caattatcaa tccaaatgtt aaattttata tccgcatgat ttgtcaggga aatggaattg
    32881 aaaaagccct ctcggttgag agggcttagc aaggaaggga ggaaacttat ttcttatcgt
    32941 tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt tcctttaatc agcttgtagc
    33001 cgaaccaaat aatcaggaac agcgggatac caatatacgt tgccgctacg ccgccccagt
    33061 caatggtatc tttcaggaac gcttcgtagt tctggcccaa agtgataatc agacacagaa
    33121 tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg gtacggcaga tcgttaatgt
    33181 cgtgtccctg caatacgtaa ccgcgacgga agcgatagtg gctaatggca atccccagcc
    33241 aggcgataaa acccgtcatc ccggaggtgt tcagcagcca caggtatacc gtctggttgc
    33301 caaacatgga ggtcaggaag cacaaaccgg caatcaccgt cgtcgcatac agcgcattac
    33361 gcggcacgcc accacgcgac aatttagcga aaatgcgcgg cgctttaccg tcacacgcca
    33421 gggtgtacag catacgagta gacgcataca taccggagtt acctgctgac agcaccgccg
    33481 tcagaataac tgcgttcatc accgccgccg cagagagcag acccgcgtgc tggaacacca
    33541 aggtgaacgg actgacgctg atgtctttaa catcgttacg cagcaggctc ggatcggtat
    33601 acggaataat caggctgata atcaggatcg cgaacacata gaacaacagg attcgccaga
    33661 atacctgacg taccgcgcgt ggaatgtttt tcgccggatc ttcggactcg cctgcagcaa
    33721 taccgatcag ctcggttccc tggaaagaga agccgacaat catagctacg ccgatcatcg
    33781 ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca gttgctccag cccgcaggct
    33841 gcgcgccttt gaagataccg ataatcatca gcacgccaac gatgataaag acaataactg
    33901 tcgtgacttt gatcagtgaa aaccagtatt ccgcttcacc aaagccacga actgagatgt
    33961 agttcagcag gaagataacg ccgaggaaca acgcactcca gatccagccc ggtgtatccg
    34021 ggaaccacca gctcatgacc agctgtgctg caaccaagtc aacggcaata gtcaccgccc
    34081 agttgtacca gtagttccag cccagcgcga agccaaagcc ttcttcaaca tagttctgac
    34141 cgtaagtggc aaacgaaccg gaaaccggca tatatgcagc caattcaccg agactggtca
    34201 tcaggaagta aaccatcagg ccaatcagca tatacgagag caatgccccg cccggacctg
    34261 cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt accgatggaa ccgccaatgg
    34321 caatcatcgt caggtgacgc gcctttaatt cacggcgtaa gcccggcgct tctgtagttt
    34381 tagtttcgga acccatacaa aaatgctatc catcttaaaa atgaggcgcg attgtagcag
    34441 acgattggca atccttccgg cagaaataca cggttataag acaccttcat gatcgcccag
    34501 ggattataag taaagcagcc aatctctttt ctggcgaatg catgctgagg gcgctgcgtc
    34561 acgaaatcga cacagcacca gcatgttctt gtacagcacc ttaacgcggt acattcgcgg
    34621 gatcgcaata gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta
    34681 tacgccacaa cgtacgcatc aggcgcggga gagggaccgc aacttcactt aatgtgcctg
    34741 cctgcaattg atcttcaatc acacgtcgcg acaggcaact aattcccaac ccatgacgca
    34801 ccgcatgttt gattgcctcg gagttaccta attccatcgc catctcaaac ttcggtaaat
    34861 gtgacagcaa cagataatcg acaatctccc gcgtgccgga accgcgttca cgcaggatcc
    34921 acggcgcagc ggccagctgt tctaaggtga ccggaccacg agccaacggc gaagtcggcg
    34981 cggcgaaaac caccagctcg tcttccagcc acggttcaga aatgatttca gtgctgtggc
    35041 acggtccttc aataaagcca atatcaacgc ggaaatccag caccgcttgc atcacgtcct
    35101 ggctgttccc aacgctaagt tcaatcggca actgcggata atcatggcga taacgggcga
    35161 taactgcagg cagaatgtag ttaccgatgg tactactggc atagatacga atcgcgccgt
    35221 tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg
    35281 gatagagcag ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga tcaaacagtt
    35341 gcacgccaag ctgcccttcc aggtcggtca aggctgcgct cactgctgat tgcgacaacg
    35401 ccagcatcac cgacgcctgg gtggttgatc cacttttcaa tacttctgca aaaacttcca
    35461 actgccggag ggtgatgtgc atagtcgctt accacttata aagattaatt ataaatatat
    35521 aatcaatttt atttttaaac cagttagtcg taaccttatg cccggttaaa ggagagggtt
    35581 atgacgaata tcaccttaca gaaacaacat cgtacactgt ggcattttat tccggggtta
    35641 gccctgagtg cagttatcac cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg
    35701 ggtgccgggt ttagtgccct caccctcgca atcttgttgg ggatggtttt aggcaacacc
    35761 atctatccgc acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta
    35821 ttacgtctgg gtatcattct ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc
    35881 ggtatcagtg ggatcatcat tgacgtgttg acgctgtcca gtaccttcct gctggcttgc
    35941 ttcctggggc agaaagtgtt tggtctggat aagcacacca gctggttgat cggtgccggt
    36001 agcagtatct gtggtgctgc cgcggtactg gcgactgagt cggtagtgaa agcggaagcc
    36061 agtaaagtaa ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc
    36121 tacccggcga tatatccgct gatgtctcaa tggtttagtc cggaaacttt cggtatctac
    36181 attggttcta ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc catcagcccg
    36241 gatgcggaaa acgcagcagt tatttccaaa atgctgcgcg tgatgatgct ggctccgttc
    36301 ctcatcctgc tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc
    36361 aaaatcacta ttccgtggtt tgccatcttg ttcatcgtcg ttgccatctt taactcgttc
    36421 cacctgttac cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt cctgctggca
    36481 atggcgatgg cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa agctggggcg
    36541 aaacctctgc tgatggcact ggtactgttt gcctggctga ttgttggtgg tggtgcgatt
    36601 aactatgtga ttcaaagcgt catcgcataa accactacat cttgctcctg ttaacccgct
    36661 atcattaccg ttttcctcca gcgggtttaa caggagtcct cgcatgaaat acattggagc
    36721 gcacgttagt gctgctggcg gtctggcaaa tgccgcaatt cgcgccgtcg aaatcgacgc
    36781 aaccgcgttt gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac
    36841 gcaaaccatc gatgaattca aagccgcctg tgaaaaatat cactacacat cggcgcaaat
    36901 tcttccccac gacagttatc tgattaacct cggacatccg gtcactgaag ctctggaaaa
    36961 atcgcgcgat gcctttatag atgaaatgca gcgttgcgaa caactggggc tttctttgct
    37021 caacttccac cctggcagcc atctgatgca gatttcagaa gaggattgcc ttgcgcgtat
    37081 tgccgaatcc atcaacattg cgctggataa aactcaaggt gtaacagcgg tgattgaaaa
    37141 caccgccggt cagggcagta acttagggtt taaattcgaa catctcgcgg cgattatcga
    37201 cggcgtggaa gataaattcc gcgtcggcgt ctgcattgat acctgccatg ctttcgctgc
    37261 cgggtatgat ttgcgtactc cagccgaatg cgaaaaaacg ttcgctgatt ttgcccgtat
    37321 tgtcggcttt aagtatctgc gcgggatgca ccttaacgat gcgaaaagta cctttggcag
    37381 ccgcgttgac cgccatcata gcctcggtga aggcaatatc ggtcatgatg cgttccgctg
    37441 gatcatgcaa gacgaccgtt tcgacggcat tccgctgatc cttgaaacca tcaacccgga
    37501 tatctgggca gaagagatcg cctggctgaa agcgcaacaa actgaaaaag cggtggcctg
    37561 atcgatgaac gacagggaaa agcagattct taaaatttta cggcgtaacc cgctgattca
    37621 acagaatgaa atcgccgata ttctgcaaat cagccgctca cgtgttgccg ctcatattat
    37681 ggatttgatg cgcaagggtt tgattaaagg caaaggatat atccttaccg agcaggatta
    37741 ctgtgtggtt gtgggtgcaa tcaatatgga tattcgcgga atggcggata tccgttaccc
    37801 gcaagcagcc tctcatccag gttctgttca ctgttccgca ggcggcgtcg gacgtaatat
    37861 cgcgcataac ctggctctgc ttggtcggga tgtgcattta atatcagcta ttggcaacga
    37921 tttttacggc gagacattac tggaagaaac gcgccgcgcg ggcgtgaacg tttcaaactg
    37981 cattcgtctg catggacaca gcactgcaac ttatctcgct atcgctaata agcaggaaga
    38041 aactatcctg gcaatcaatg acacacatat tctgcaacag ctaacgcctc aactgctcaa
    38101 tacttcacgg gacttgattc gccatgctgg tgtggtgctg gctgattgta atctcacacc
    38161 tgaagccctg gagtgggtct ttaccattgc tgatgaaatt ccgatgtttg tcgataccgt
    38221 ttccgaattt aaagcgaaca aagtaaaaaa ctggtacagc cggatccaca ctctgaaacc
    38281 aacacaaaat gaactggaaa ttttgtgggg gcaaccgatt aaagacgaca acgatcgcat
    38341 cagagctgta aattcgctgc atcaacaagg tgttaaacgc atttttgttt atctgaaaga
    38401 tgaatctgta ttttgcagcg ataaggatgg agaacaattt ttgctgaccg cgccagcgca
    38461 tacgacagta gacagttttg gtgctgacga tggttttatg gcgggcctgg tatatagctt
    38521 tcttgaagga tacagtttcc gcgacagcgc ccgttttgcg gtagcctgcg cggcaatttc
    38581 gcgcgccagc ggcagcttaa acaaccctac cctgtctgcc gataacgcgc tttcattagt
    38641 gccaatggtg taacaatgtt gccggattcg acgcttgacc gcatcttatc cggctcataa
    38701 attgcgcact acgctaaacc aataaagaat cctgcaatag tagcactcat caggttagaa
    38761 agtgtcgccg ccgccagcgc gcgtaaacca agttgggcga tttccggcgc acggtgtggc
    38821 gcaaccgcag aaaacgcccc caccaccacc ccgatagaac caaagttagc gaaaccgcac
    38881 aacgcgaaag aaataatcgc cacggtttta gcatccagag tgccacccgt ttgcagatat
    38941 ggcgagaaat tgagataagc gacaaattca ttgatcgcca gcttctgccc aatcaaactc
    39001 ccggcaagat ttgcatcact ccagtcaacc cccatcaccc acgccaatgg ggccaacagg
    39061 taacctaaaa tcgactccag cgaagcatgt tcaaaaccaa accagccgcc aacaccgccg
    39121 ataataccgt taatcaacgc aattattgca acaaatgcca taacaactgt cgctacaccg
    39181 gcggcgattt tcagcccggt cattgcccct gtcgcagcgg cttcaataat gctttttggc
    39241 ggtgtttcgg tgaaagagag gttattaaaa gaaacctgcg aagattccgt agccgggctt
    39301 aacaggcggg caaacaagat cccgccaggg atcgccatta acgatgccgc cagcaaatat
    39361 tccacaggta cgcccagtgc ggcgtaacca atcattgtcg aaccagcaat cgaggccatg
    39421 ccactacaaa tcgctgtaaa taattcattg cgattcagac gatcgataaa gggtttgacg
    39481 atcgccggaa tttcgttttg cccgaggaaa atggtggtga ccgcgacgaa tgactcgatc
    39541 ttgctgatat ttaaagcttt ctggaagata ccgccgagaa tgcgaattaa gatccccatc
    39601 acaccgatgt agtagagaat actcaccagt gcagtgacga agataattgc cggtaatacc
    39661 ctgaaaccaa agataaatcc tgcgccatca aataaggtat ccattttcgg tccgaccaga
    39721 gaaccgaaga taaatgcgct acctgcgtcg ctgtacgcca tcactttgtg gacgccaaaa
    39781 gcgacttttt ctgcgaccca acgccctggc ggtaaccaaa gcataatccc gccaatcacg
    39841 acctgtaaca ctaacgccgc gccaacggta cgcaggctga tcttcttctt gtttactgac
    39901 agtaaaaacg caattgccag cagtaccacc attcccagaa cacttctcat gacatccata
    39961 gtgattatct cttcatgtca ttttgggcga cggctttctg cccatcagaa atagccgggc
    40021 ctgcatcttg cgatactgtc ccggcttgtt atcaacactt aatgggtttt tatgtagccg
    40081 cgtacgcact cttcgactaa tccccagaac cagtcggtat caatagtgat gccgacttta
    40141 gtattggcgg gcttgccaag aacgcccagc tcgtcgcaga cggtacgccc atagcaaggg
    40201 ccactgttga cgtccacttc gacgtacatc tcctgggttt taatgccatc agggttaatc
    40261 agataaccga tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta gttttcgaac
    40321 tgcgttttga gagtgaagtt catgatgtcg ctgaacatct ctccggcagg gccgcctgcc
    40381 ctttccattc gtgcaattac gtccggggtg caaacggtct ggttggtaag atcgaggccc
    40441 atcatcacta atggaacgcc ggaggtgaac accacgcgtg cggcttccgg gtcggcaaag
    40501 atattgaact cggcagatgg ggtgaagttg cctgtaccgt aagcgccgcc cattagcaca
    40561 atttcacgga ttttgggcag gattgcgggt tgcatacgca ttgccaccgc gatatttgaa
    40621 agcggaccaa ccggcaccag agtgatatcg ccatcgcttg ccataagggt atcaatgatg
    40681 tatttcaccg cgtgggtact ttctgcctgg cgggtcagcg gctcgaatac cgggccatcc
    40741 agtccggttt caccgtgaat attatcggca acgatttgtt gacgcataat gggttgcggc
    40801 atccccgcat aaaccggaac attaatctcc agtttctggc aaacattcag gccattaatt
    40861 aatgttttat caagcgtctg attacccgca acaatggtga tacctaataa atcaatcgcc
    40921 ggatgtttcg ccgccatcat tatagcaata gcatcatcat gacccggatc acaatccaga
    40981 ataatttttc tcttttccat tgtttatttc ctctgtttcc agttgcgtta ttttttctac
    41041 agcaacgaaa tttcgatcgg gccgatgatt gaatcttaac aacagcgtac gaatgctaaa
    41101 tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat gatcttaagc
    41161 agcaattgat gagtgaatcc gcgtttaagg attgcttttt aacggatgtt tcagccgata
    41221 cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa cagccgtcct
    41281 ggctgtttta cctgacgcga ggccgcgcca ggctttacgc cacgctagct aatggtcgcg
    41341 tgtcgctgat cgatttcttt gccgccccct gttttattgg cgagattgag ttaatcgaca
    41401 aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt gcgctcccta
    41461 tgaaacatta ccgcccgctg ttattaaacg acacgctatt tttacgaaaa ctctgcgtca
    41521 ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa tcatttccgt
    41581 tagtgaatcg cctggcagca tttatattac tctcgcagga aggtgatctt tatcacgaaa
    41641 agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta tatgttctcg
    41701 cgcaattcat tcacgacggt ttattaatta aaagcaagaa agggtatcta attaaaaaca
    41761 gaaagcagtt gtcaggactg gcgctggaga tggacccgga gaataaattc tccgggatga
    41821 tgcagtaaaa attattggca atagcacgat tgccggatgc aacgcttaat aaggcgtaca
    41881 tccggcaatg aaactcaagc taaaccaata aagaacccgg caatggtcgc gctcatcagg
    41941 ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct gggcgatttc cggcgcgcgt
    42001 tttggcgaaa tagccgaaaa cgctccaacg acaacaccga tagaaccaaa gtttgcaaac
    42061 ccacagaggg cgaacgagat aatggcaatg gtcttgacat ccagcgtgcc gctcgtttgc
    42121 agatagggcg agaaattgag ataagcaaca aactcattaa tcgccagttt ctgcccaatc
    42181 aaacttccgg caagatttgc atcactccag tccaccccca taatccatgc caacggggcc
    42241 agcacatagc cgaaaatgct ttccagagag acattggcaa aaccaaacca gccgccaact
    42301 ccaccaataa tgccgttgat cagcgcaatg atcgctacaa atgccattac caccgtcgcc
    42361 acaccggcgg cgattttcag cccggtcatt gcaccgttcg ccgcagcttc aataatgctt
    42421 tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa cttgcgaagg ttccgtcgcc
    42481 ggactaagaa tacgtgcaaa caggataccg ccaggaatag ccatcagcga agcggctaac
    42541 aaatagtcaa tcggcacccc cattccggca tagccaatca tcatcgaacc cgcaatagac
    42601 gccatgccgc tacaaatagc cgtaaatagc tcgttgcgat tcatgcgatc aataaaaggt
    42661 ttaacgatcg ccgggatctc attttgcccg aggaaaatag tggtgactgc gacaaaagat
    42721 tcgattttgc tgatgttgag ggctttctga aaaatactgc caagaatacg aatcagcagc
    42781 cccattacgc caatgtagta cagcaagctg ataagcgcgg taacgaaaat aatcgccggg
    42841 agtacgcgaa aggcgaagat aaagcccgca ccgtcaaaca gaacatccat tttgggaccg
    42901 accagcgaac caaaaataaa ggcacttcct gcgtcactgt aggacatcac tttatggacg
    42961 cctaatgccg cctgctctac tgcccatttt cccgggggaa aatagagcat gatgccacca
    43021 atggcaattt gcagcagcag cgcagcgcca accgtgcgca agctgatgct ctttttattt
    43081 accgacaaca caaaggcaat tgccagcaat accaccatcc ccgcaacact tctcattata
    43141 tccataatga ttttcccttc atgccagtaa acccggcgtc agcgccaggt tttggtatgt
    43201 ttgatgagta cgggcgacgg ctttctgccc gccagaaatt aacccacgag acgctgatat
    43261 tctttggcaa tttcgctcgc cagaatggcg ttattaaaga ccagttgaat attggatttc
    43321 aggctatcac cgccggttag ctccgcaaca cgcgccagca ggaacggtgt actctctttc
    43381 ccaataacac cctgttcttc ggcttcagca acggcttgat caattgctgc attgatggtt
    43441 tcttctggca tcgcaaactg ttccgggatc gggttcgcta ccaccaggcc gccgttcaaa
    43501 ccggtttgcc acttcactgc catcgcgcga gcaatttcgc gggcactgtc aagacgaatg
    43561 ctgacttcaa atgggctggt tcggcagaaa aacgcaggca gcgcttttgt ctgataacca
    43621 attaacggta caccgaaggt ttctaaatat tctgttgtta atcctaaatc gagaatagat
    43681 tttgcccccg cacaaacaac agtgacattg gtatttgcca gttcctgtaa atcggcagaa
    43741 atatcgaagg tatgttccgc tccgcgatgc acaccaccaa tacctccggt tgcaaaaact
    43801 ttaatcccag ccagtgctgc aataatcatg gttgatgcaa cggtagtcgc gccatttttc
    43861 cctgcagcaa cgacaaaagg taaatcacga cgactaactt tcgtcacgtt atgtccttca
    43921 cggcccagca attcgatttc ttctttactt aagcccactt tcatcacacc gccaataatg
    43981 gcgatagtgg ctggtactgc gccctgttta cgaattgttt cttctacttc aattgcggtt
    44041 tgggcatttt gtgggaatgg cattccgtga gaaattatgg tcgattccag cgcaacaacc
    44101 ggttttttat tttttaaagc atcctgtact tcagcggata tttgtaataa ttcaggggac
    44161 agagttaatt cagacattct gtgttctcca ctaacgatct aacattggca attgataatt
    44221 cagggttgtt ggtatattca caggcaagtg ccattgacga acatccctgc gcaaatcgaa
    44281 cagaatcgat aaacggcata ccgtctaccc aacaggaggc cagccccgcc atcatggcgt
    44341 cgcctgcccc agtaacattg atgacattcg ttttaatagg ggccgaccac ccactttcac
    44401 cgttgatatc actgtagtaa acgccatccc ctcccatgct cagcaccaag cggttcaggc
    44461 cgtgttgatg aaaccaggcg gcgacctttg cgacatcttc acgtccggaa agtgcaattc
    44521 cgctcaacgt ttcggcttca agacggttag gttttaacgt atggattttg ctcagatgat
    44581 cgcggatttt gacgcatttc catgccgata ccggatcaat aaatacaggc gtttccccgc
    44641 tattttccag aacccagacc agcacctctt cactaaggtt gcaatccgcg acaataacct
    44701 ttgcaccgcg aataaactcc tgatgctggg caagaaattc tgcagaaata cagtcactaa
    44761 tactcatatc atttatcgcg acaagcattt ctcctgtatt atcgagcagt gataaatagc
    44821 tggatgtatt ctcacccgga acaattaagc atttatcgac ataaacaccg gattgattag
    44881 tttgcgccag taatgattgc ccataaaaat caccacctac tgcacttaaa agccaggcat
    44941 ttttccccag taacgccaga ttatgagcga tattccttcc cacgccacct ggggtaaatt
    45001 taatttttcc gggattcgaa tccgcataat tcaatggatg atgtgaatac ccagccacat
    45061 caatattcgc cgaaccaata attactatgt agtccttttc gcgcatagca cgccccctgg
    45121 caataaattg ccaacgaaga aattgggtaa aatagccatt acagtcaata agactattag
    45181 agcacgtgtt cagatttaaa cacatgttca taataagcat atttaacgta atgtaaatcg
    45241 atcagaaaaa acgaaatcgg ccagatatga aggcggtcac aaagtttgtc tatttatgca
    45301 gcaacaaaag agaaagttgt gatgtgtaac acacaatatg gcaggcaaga aaaagggcag
    45361 agatgtttct gccctccaga ttgttgacaa cgcgcgcttt gttcatcctg gatgtggcgt
    45421 aaacgcctta tccgacctac aaaagcgcaa aaaattcaat atattgcagg gttcatgtag
    45481 gtctgataag catagcgcat caggcaattt tgcgtttgtc atcatcgggg cagagatgtc
    45541 tcatccccga taatgaagat aaattatgcg tttttttcta ctatttgtgt ttccgggcgt
    45601 ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc taccaggtaa
    45661 cccaataccg gcgtaatagc gccagggatc agcagaacaa acagaccacc gtgtggtgcc
    45721 atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc aacgatacag
    45781 cacggtagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc cgaaataaag
    45841 cacagaccaa gtaccagagc ggctttgcca ccttcctgct gcgctttgtc gaatttgcga
    45901 cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc cgccataatc
    45961 gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc gtacgctgct
    46021 ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc ccccagcaga
    46081 accgcattcg cagtccccat ggtctgcagc cagtgagtca acccatcgag aatgccagca
    46141 accggtttac cgattaggta gatcatcgcc agaccgacca ccagactgga aattagcgga
    46201 atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcaattg cgtactgatt
    46261 aacttcgcaa tgtaaccagc caggaagcca gcaattatac cgccaataaa gccagaaccg
    46321 gtgctgaccg ccagcatacc gccaatcaga cccggtgtga ggcccggacg atcggcaatg
    46381 gagaaggcaa tataacctgc cagtaccggc accatcagcg caaaggctga accaccacca
    46441 atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat accaaaagca
    46501 aaagaaagcg cgatacacag accacctgca acaaccatcg gcagcatgta agagacgccc
    46561 gtcagcaagt gacggtaagc gcctgcactc tctttcttac cttcagtggt cgctgtttga
    46621 gctttgcccg ctggttcata cggcgttgct tcagcaaccg ctttatccag ttcctgcgca
    46681 gttttcttca gcgccagacc ggtagaggtg cgatacatcg gtttaccagc aaatttcgcc
    46741 agatccactt cgatatctgc cgccacaatc accagatccg ctgctgcgac ttcttcaggg
    46801 gtgattgcat tgcccgcgcc aacagaacca cgggtttcaa ctttcaccca ccagccacgt
    46861 tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac gccagtcggg
    46921 caagcagtca ccgcaactac gcgtttcgga ccgctggcag caaccggtgc tgtcgcagcg
    46981 accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag ctcagggtgc
    47041 gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc gctgtcattc
    47101 gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc
    47161 agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc gcgtgcctga
    47221 ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa
    47281 aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg taatacccac
    47341 attgctttga cttaccgcca gggctgcaac agctgtcgcc agacgcagtg tgtgttcact
    47401 ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc ctgcgccaac
    47461 ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg cattaaccca
    47521 aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt cacgtagcgc
    47581 atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc agatttccaa
    47641 ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta ctaacgcttc
    47701 acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca tccagtcggt
    47761 gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca tatcgaactg
    47821 accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcgaggg tgacttcaaa
    47881 acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg
    47941 ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga acagttgctg
    48001 aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc
    48061 ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg tggttttcac
    48121 caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat aagccggatt
    48181 aagggtgatc gtagcaacac gtctgctcat tatgcgccct ccccaagacc agcagcgata
    48241 gcgtcgccaa tggctttcag cgcctgttca gcatctgcac cctgggcggt aaagcgtagg
    48301 cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc gtttgccggt
    48361 ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt aatggtattg
    48421 accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg
    48481 cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca
    48541 tccgctttca gcaagcggtc agctttattg tcgagcaata aatcagcgag acgcttaaga
    48601 accgcgatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc cgtttcgccg
    48661 tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag attgccttcg
    48721 gcgctatcgc tcagccagat accctgtccg agattcagcg gttgttcatt gatggctttg
    48781 gtgacgaaag tggcgtcaac tgcccccacc tctttcagac gcgcagcgtt cagcgcctga
    48841 agagtcagca gatcgctggc gacgatatcc agcgtcagca tttcgttgtc gagcttcagc
    48901 tgctcactct gcttttcgcc catcagtaat gcgcgaagtt cttctgctgt tgttgctgac
    48961 ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg tagcaggccc
    49021 agatgttcat ccgagctggc agcaataccg attgccacgt acgctacctg accgtcaccc
    49081 caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg
    49141 gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga ggtttgctgt
    49201 tcgcgcgcca gcatgccatt gacgtagcct tctgctacat taccggcctg caccagcgcc
    49261 gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg atggatgtcc
    49321 tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat tcagcctcta
    49381 tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca
    49441 agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt
    49501 ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa atccgttatc
    49561 agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg cattttgatt
    49621 attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg taaactccgc
    49681 gtcttcctct tccagtgatc gacctgcatg cataactccc ccgcagtctc cagcgcgaaa
    49741 tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg tattgcgggt
    49801 gctctgcaaa ccccgacact cagtattttc cttaccgatg aagtacatgc ccgtccggcg
    49861 atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag tcagtttctc
    49921 gctgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta
    49981 ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt gctattcgtt
    50041 ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt
    50101 gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt acgcgctcag
    50161 gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat gggtttcagc
    50221 tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt gatggtgtgg
    50281 ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt tgaagcgccg
    50341 cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat gtggggctcg
    50401 aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca tcttcccgag
    50461 aaactggccg atgtgatgat ggggaccgcc gccgggctgg aaatcccgac gatgttgatt
    50521 gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt
    50581 ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct gttgggcttg
    50641 cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct ctattttcag
    50701 gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac ttcgcgcgtg
    50761 ggctggatca tcgcaggatc agtggcgggc atcgtcgccg aaatctggaa ttatcacgct
    50821 gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact gcggattaaa
    50881 gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc ctgctgacgc
    50941 gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg tcgcagtggc
    51001 gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt attggcgggc
    51061 ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca acacgccccg
    51121 caacccggac ggcattccat acgttgttct cttaaggtta accaccggat tgatgagcgc
    51181 gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc gaaaggcccg
    51241 tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt ttcgatgcca
    51301 agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact gctgctggtt
    51361 aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt
    51421 ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga tgatatcgtt
    51481 gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg caacgaatat
    51541 gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca gattgaagaa
    51601 gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac ctgggacggt
    51661 caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga aaccgcacca
    51721 ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag cactggtctg
    51781 gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa
    51841 cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg atagctcagg
    51901 ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga acaccttacg
    51961 ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa caatccagac
    52021 caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg ttactaagac
    52081 gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca cgctccacgc
    52141 tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc ctttcaccgc
    52201 gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc
    52261 tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc acagaaccat
    52321 ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt cggtgccgtt
    52381 cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa attttgcgag
    52441 ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat tgacccggcc
    52501 accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac ccctgaagaa
    52561 cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt
    52621 acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt ggtgaaaaac
    52681 gcggtgctgg gaatggcaga aaaatgttcg ccagaactcg ccgggtggat aaaaaaacac
    52741 gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg
    52801 gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg cgaaccgttt
    52861 atccagtggg tggttgaaga taacttcatc gctgggcgtc ctgcctggga agtcgcaggt
    52921 gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat gcttaatggc
    52981 agccactctt ttctcgctta tctgggttac ctctcaggat tcgtccatat cagtgattgc
    53041 atgcaggatc gcgtatttcg ccatgctgcc agaacattaa tgctggatga gcaagcgccg
    53101 acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat tgcacgtttt
    53161 gctaatccgt cgctgaaaca taagacctgg caaatcgcga tggatggcag ccagaaatta
    53221 ccgcaacgta tgctggcagg tattcgcata catctggggc gtgaaacgga ctggtcgttg
    53281 ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat
    53341 gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc gggcagcagc
    53401 agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca
    53461 gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt acaattcggc
    53521 gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt aaagcggatg
    53581 aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca ggattaacgc
    53641 tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct catcaccggt
    53701 tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa agatcccaac
    53761 gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg tgctttgctc
    53821 gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg cgttaatggt
    53881 ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg
    53941 atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac cgcaccagtc
    54001 tatgaactgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg cctgctgctg
    54061 gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc agacatcatt
    54121 gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca gcgttggtgg
    54181 cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt tgacggtcat
    54241 cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc gcattctcat
    54301 cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca acgctggcgt
    54361 cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga cgctgatacg
    54421 gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa
    54481 ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga tgacctgcac
    54541 attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc cagcagcgaa
    54601 gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa
    54661 ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat ttgccgcaaa
    54721 tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc
    54781 atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag aaactggtcg
    54841 aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt
    54901 ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac gcccctggca
    54961 gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta ttaaaccagc
    55021 tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt accgatatta
    55081 accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat agctttcccg
    55141 gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat ttcggcaaag
    55201 ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga gtaatgattg
    55261 gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg atcggtttgc
    55321 cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac aaatcactac
    55381 cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc atcaggctta
    55441 tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc gcgcgcaaaa
    55501 atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac tttaattgac
    55561 caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg taaaaccgct
    55621 cgccaagacc gcaccaatcg ggtaatctag aactcgtttt gcctcggcgg tagattatcc
    55681 tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat tgtttcaaca
    55741 tgcccaggta attagtctcg tgtcgcttgg cattttttta taacgatatt tgtcgttaag
    55801 gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga tatatcttgc
    55861 gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca aataacaccg
    55921 caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca ctgcaagctt
    55981 ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga attatggatc
    56041 agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg
    56101 attgttctgg ttttgtacaa cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt
    56161 cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg
    56221 atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat atcggcaaca
    56281 atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg aatgaaccgt
    56341 actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa taaaccgttt
    56401 ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag gcagtgcttt
    56461 tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg
    56521 tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc atgttcatac
    56581 gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgtagcaggc gtgatgattg
    56641 cgatactggt tagctgcctt cagtttttag tggcctggca taagcacgaa gtcaaatacg
    56701 acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac ctgaaatcca
    56761 ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac cccgaactga
    56821 ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa
    56881 cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg attccggcgc
    56941 tcaacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg gtgccgaaca
    57001 aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt gtgtttgccg
    57061 ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa gattacgatg
    57121 gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt ttgatgaacg
    57181 ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg
    57241 ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt ttactgggcg
    57301 gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta cgtatgcgcc
    57361 ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg
    57421 tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc tggcggcatc
    57481 ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg caaaagatga
    57541 ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca gaattagaaa
    57601 aagtgctgcc agtaggcgtc aaatttggta ttaacattgc gccggaccat ctgcacagcg
    57661 aaagctttaa agcagatatc cagaaactgc tcacttccct gcccgcacac catttccaga
    57721 ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca caactcttcg
    57781 cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc gggcacagcg
    57841 cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt ggatttatca
    57901 acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg acgctggcga
    57961 aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac gccggaacag gcgcgatggc
    58021 taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgccct ttaccgctgg
    58081 acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt
    58141 cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta aaaggaaatc
    58201 cattgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc tttggtgtgc
    58261 aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt
    58321 ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt
    58381 tgtcagccct cggcaccttc gataatttca accgctatgc actgcgcggc aacccgggcg
    58441 cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa ccaggcagtt
    58501 attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg gtggaggtcg
    58561 ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat tactgcccgc gatgtagagt
    58621 ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca
    58681 ccaccgtcaa agccattgca ccgttaaccg tgcgcattga gttagctaaa cccagcaaag
    58741 aagatatgct gagtctgttt tcgctgccga tatttccaga aaagtactgg aaggatcaca
    58801 aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccgc gttacgtcct
    58861 ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca gcaaacttac
    58921 cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac ctcgatgata
    58981 atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa aacgacgcta
    59041 aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc atcaaagatg
    59101 agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc caacgtccgg
    59161 tattcagcga tcgccgggtc cgggaagcaa tcactctcgc ctttgacttt gaatggatga
    59221 acaaggcgtt gttttacaac gcctggagtc gcacgaacag ttattttcag aataccgaat
    59281 acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca atgaaaaaag
    59341 atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatctaaa ggcgatggct
    59401 acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaaacgggc tgggtgctga
    59461 agggtcagca acgcgttaat gccacaacgg gtcagccgct cagctttgaa ttattgcttc
    59521 ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa cggctgggta
    59581 tcaatatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg cgcagtcgcg
    59641 actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc gatttacaga
    59701 tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg caaagcccgg
    59761 ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa aaattactgc
    59821 cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt
    59881 acatggcgga agaccgtctc gcctggtggg ataaattctc ccaacccgct gtacgccctg
    59941 tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc aaactgccgt
    60001 cagccagaca acagggagag tagatgggcg cttacctgat tcgccgtctg ttgctggtga
    60061 tcccaacgtt atgggctatt atcactatca actttttcat cgtgcaaatt gcgcctggcg
    60121 gtccggtcga tcaggccatc gccgccattg agtttggtaa tgccggagta ttacccggcg
    60181 caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat atcagcgaca
    60241 gtaattaccg tggcggacgc ggattagacc cagaagtgat cgctgagatc actcatcgct
    60301 acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac tacatccgct
    60361 ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt aaagacagtc
    60421 tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg gtgtcgattc
    60481 cgttaggcat tcgcaaagct gtttataatg ggagtcgctt tgacgtctgg agtagcgcat
    60541 ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg attgtcttct
    60601 tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc gctaactttg
    60661 attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg ctaccggtgc
    60721 tggcgacagt gattggtggc tttgcggcgc tgaccatgct gaccaaaaac tcattccttg
    60781 atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt gagaaaaaca
    60841 ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt tttccggcga
    60901 cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt tcactcaatg
    60961 gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta atgtttggta
    61021 ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat atcagctata
    61081 cgctggttga tccgcgtata gattttgagg gacgttaatg tcgcgactca gccccgtcaa
    61141 tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt tatggatttt
    61201 cctcgttttg tttggtttga gtttgtgttc tgaacttatc gctaacgata aaccgttgct
    61261 ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg aaagcgattt
    61321 tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac aacggctgga
    61381 aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta tcaactttgc
    61441 taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa cggatgccaa
    61501 cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc tgtttggcct
    61561 gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac aaggctatta
    61621 cggcggtaaa gtcgatctct ggggacaacg ctttattgaa gtatggtcgg ggatgccgac
    61681 gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt ggctgctggc
    61741 aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg
    61801 tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg atcgcagtat
    61861 catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt taccgtttat
    61921 tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg gtctaccgct
    61981 cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc aggccccgtg
    62041 gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga tctttattgg
    62101 tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg caaactctgt
    62161 tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt acagtagtca
    62221 atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt gagtcaggtt
    62281 caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg ccggttgaat
    62341 atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc gatcaaacgt
    62401 tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg gtgtcgttaa
    62461 atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac cgcgggatgc
    62521 gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgtcggt atccgccagg
    62581 cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc cagcgggtga
    62641 tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa ccgaccaccg
    62701 cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg caaggcgagc
    62761 tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa ctggcccacc
    62821 gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg
    62881 cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca ggcgatccgg
    62941 taccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag gttgccttcc
    63001 ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg aaaaacatca
    63061 gttttacgct acgggcgggt gaaacactgg gtttagtggg cgagtctggt tccgggaaaa
    63121 gcacgacggg actggcgctg ctgcgactga ttacctctca gggcagcatc gtctttgacg
    63181 gtcagccact gcaagattta aatcgccgcc agctgttacc tactcgtcat cgtattcagg
    63241 tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg cagattattg
    63301 aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa caacaagtga
    63361 tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat ccggcggagt
    63421 tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt aagccctcac
    63481 tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg caaatattga
    63541 cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc agccacgatt
    63601 tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg
    63661 aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg cgtcagctac
    63721 tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc aattgccaca
    63781 ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac aaacaggcac
    63841 ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa cgcctggctt
    63901 agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt aagcgccacc
    63961 aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga acctgcgaaa
    64021 ccagcagacc agcgaaagtt ctgcggtaga cgatggacga ttgagagttg taatgaagtc
    64081 acttattttc cccggaagca catttacttc acaatttatt tacatcaatt ttaacacatc
    64141 atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgca tggcctctat
    64201 ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt
    64261 ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca taaattgatt
    64321 ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat gcatcaacaa
    64381 aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggt cgactggcgt
    64441 acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc atcggccatg
    64501 cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg ccaaaacgga
    64561 aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca agaatgaccg
    64621 ccatcgcatt ggatgacacc atagacacgc agcccacaaa cgccgcaacg ccaaccacca
    64681 gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc ataataaatt
    64741 gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcggacg aagcggctgt
    64801 taaagatggt catcacgaac agaaaaacaa tgtttagcgc aaagtaataa ccaaagtttt
    64861 ccggtgcaac gtggttgatt tcaatgtaga caaacggtcc ggcgctcagg aacgagaaca
    64921 ttccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta tgacggaaca
    64981 gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgagga tccagaagat
    65041 gtaatgccag ctcagccaca ccagcaccca gccgccaact atcggtgcca tcagcggtgc
    65101 aatggtcgtc accagcatga caaacgacat catccgcgag aactcttctt tcgggtaaat
    65161 atcacgcatc aaagcgttga taaccacgct ggccgcagcc gcagccagcc cgtggaagaa
    65221 acgcatcaca atcagctgat cgatggtttg tgccaacgca cacgccaccg cggcggcggc
    65281 aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg ccatcggccc
    65341 gtagattaac tgccccaacg caaagcccag aatataagta ctcagggtca tctgcgtact
    65401 gcccgccggt acgccaaact gcgctgaaat taccggtagc gcgggcagat acatatcaat
    65461 cgacagcggc atcaacatgg ccagcaggcc aaggataaaa acaatagcaa acgacgaatg
    65521 ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa agttagacga cgctggcaat
    65581 ttcttcttca gttaacggac gatattcacc gggggctaaa tcagcatcca gcgtaatgcc
    65641 gccaatacgt tcacgatgca gctcaaccac atggttaccc acggcggcga acatgcgttt
    65701 cacctgatga taacgccctt cgctgatggt cagacgaacc tgcgttgggg taatcacttc
    65761 cagcaccgcc ggcttagtga gatctttttc gttatgcagc tgcacgcctt tagcaaattg
    65821 ttctgccgta tcgtcagcta cgggtgatac cagcgtcacc agataggtct tctcgcaatg
    65881 atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta gtaccagacc
    65941 ggtggtatcg atatccagcc gccctgcggc gtgcagtttc cacgctaccg gttcatctag
    66001 aaaatagagc accgttgggt gatcagggtc gtccgtggag caaacataac cctgaggctt
    66061 attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac
    66121 atcatgttca ggaagcagtt tgaacgctgc attacggacg atttcgccat cgacggtgac
    66181 acgattgccg cggatttcac gcccggcaat agcacggcta acgccgagtt gctgtgcgat
    66241 aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac
    66301 ccgaatatct gaactgttgt ctgcccagta tagcggtcta ataacatccc tcaagggaaa
    66361 aagatccgtg gcatactatt agcagaataa tctacctacg cgagaccatg atttttacac
    66421 ttcgcccata tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa
    66481 cccctgccgt tatcgtgctg cccaccggtg caggtaaaag cctggtgata gcggaactgg
    66541 cacggctggc tcgtggtcgc gtactggtgc tggcacacgt taaagaactg gtggcgcaaa
    66601 accatgcaaa gtatcaggcg ctggggctgg aagccgatat ttttgccgcc gggctgaagc
    66661 gtaaagagag ccacggtaaa gtggtatttg gcagtgtgca gtcggtcgcc cgtaatattg
    66721 atgcctttca gggtgaattt tcgctgttga ttgtcgatga atgtcaccgt attggtgacg
    66781 atgaagagag ccagtatcag caaatcctca ctcacctgac aaaagtgaat ccccacttac
    66841 gcctgctggg gctgactgcc acgccttttc gattgggcaa aggttggatc taccagttcc
    66901 attatcacgg catggtacgc agcgatgaga aagccctttt ccgtgactgc atttatgagc
    66961 tgcccctgcg ttatatgatt aaacacagct atctgacgcc gccagaacga ctggatatgc
    67021 cagtagtgca atatgatttc agccgcttgc aggcacagag caacgggctg ttcagcgaag
    67081 ccgatctcaa ccgtgagctg aaaaaacagc aacgtattac cccgcacatc atcagccaga
    67141 ttatggagtt cgccgaaaag cgtaaaggag tgatgatttt cgccgcgacg gtcgaacacg
    67201 caaaagagat tgtgggatta ctgcctgccg aagatgcagc actgattact ggcgacaccc
    67261 ccggcgctga gcgcgatgtg ttaattgaaa attttaaagc ccagcgtttt cgctatctgg
    67321 tcaacgtcgc ggtactgacc accggatttg acgccccgca cgtcgatctt atcgccattc
    67381 tgcgccctac cgaatcagtg agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg
    67441 ctccgggcaa gactgattgt ttaattcttg attatgcggg taatcctcac gatctctacg
    67501 cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc
    67561 ctgcctgcgg ttttgccaac accttttggg ggaaaacgac cgccgacggg acattgattg
    67621 aacactttgg tcgccgctgt cagggatggt ttgaagatga cgacggtcat cgcgaacagt
    67681 gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa cgcagaaaat gatattgccg
    67741 ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc ggatgatatg ttaaaagcgg
    67801 cgctacgact gaaagatgcg ctggtattac gctgtagcgg catgtctttg cagcatgggc
    67861 acgacgagaa aggcgaatgg ttgaaaatca cctattacga tgaagacggt gcggatgtga
    67921 gtgagcgttt ccgtctgcaa acacctgccc agcgtaccgc cttcgagcag ctttttatcc
    67981 gcccgcatac gcgcacaccg ggcatcccgc tgcgctggat caccgccgcc gatatcctcg
    68041 cccagcaagc cttattgcga cccccggatt ttgtcgtcgc ccgcatgaaa ggccagtact
    68101 ggcaggtgcg tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac
    68161 gcggttaatg gtagttttga ttgatgtata aggcgattga gtatagaatc tcacccgctt
    68221 ttgcatacgc aaagcagatc acttacctgt tgctgggtcg cctgtagcag gattaattta
    68281 agagagaaag aaatgtttac tatcaacgca gaagtacgta aagagcaggg taagggtgcg
    68341 agccgccgcc tgcgtgccgc taacaagttc ccggcaatca tctacggtgg caaagaagcg
    68401 ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca tgcaagctaa agctgaattc
    68461 tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac
    68521 gtacagcgtc acccgtacaa accgaagctg cagcacatcg acttcgttcg cgcttaattg
    68581 ctgaataagt tgtaaaaaac cccgctcccg cggggttttt tgtatctgca gattatgcct
    68641 gattacggta ttgctatttt ttgcaggcca gataaggcgt tcacgccgca tctggcattc
    68701 agaacacaaa accgattaat tgccgccaga cgtccggcgc tgcaattggt cgcgcaaatt
    68761 cggcggcgtg cctttaatgg tcagggtatc ggtcgccggc tcccagaaga tgcgttcacc
    68821 taacagcatc gcatcaaagt taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa
    68881 ctgacgcagt gtgctccggt ctgccgggaa gctctcttcc agttcgtagc ctttttcagc
    68941 cgcaaactcc gtgaagctca cttcgcttac gcctgccagc tctttcgaca gcgattccag
    69001 ttcgatctct tcgcctgcct gcagctgctc attacagtag ctataaacct gctggcgcac
    69061 gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg aaatcatcaa ccgcttgcaa
    69121 cagcccacgg ttttgtgctt tcgtgttcaa tccttcgctg gccccgagga aatccataaa
    69181 gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa gtgagatagc gggtggactc
    69241 tggattggtt tcccattcgg ttaaatcgat acgcgcaaca atatccgcat gattgatatc
    69301 aagataatgg gttgggttga tgtcgagatt ttcgttaaca cgcatactgc tcaggttgct
    69361 cagaactgcc accagcagat actccaccgc cagataacgg tagtggcaaa acagcacgaa
    69421 tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccag ttgccgcgcg
    69481 gctaaatgcc agaaagtcct cttcgccctg acgctgtaag cgcagcgtct gcgccagttc
    69541 actctcttcg ctaaacaagc cgtaagcttt atttttggcg ctatagaccc gatgcagttc
    69601 tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat gaatcgcgca acaccagctc
    69661 aaggttctgc tcatcacgct tgataagctg gtgcagagca atctggttga tatccagact
    69721 catgataaac tctcctttaa gaccgggcgg tattcaacca ccgccggtta agcgacgcaa
    69781 taaaagatgg cggtcatttt acagaaggca gcgtaacgag aatgatttaa cgcataaaaa
    69841 agcagaaaaa aaaccgttgc tacggtaata tgttgccctt tcatcaacta actgatttcg
    69901 atttatgcca caaatttccc gctacagtga tgaacaggtt gaacaattgc tcgcagagct
    69961 gctcaacgta ctggaaaaac ataaggctcc gaccgacctt tccctgatgg tgttaggcaa
    70021 catggtcact aaccttatca acaccagcat tgccccggcc caacgccagg caattgccaa
    70081 ctcttttgcc cgcgccttac agtcctctat caacgaagac aaagcgcact aagggaaaca
    70141 gataacaggt tatggtaact catcgtcagc gctaccgtga aaaagtctcc cagatggtca
    70201 gttgggggca ctggtttgca ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt
    70261 acctgtttat cgccgactgg ccgacaacgc ttgctggtcg catttattcc tacgtaagca
    70321 ttatcggcca tttcagcttc ctggtgttcg ccacctactt gctgatcctc ttcccgctga
    70381 cctttatcgt cggctcccag aggctgatga ggtttttgtc cgtcattctg gcaacggcgg
    70441 gaatgacgct attactgatc gatagcgaag tctttactcg tttccatctc catcttaatc
    70501 ccatcgtctg gcagttggtt atcaacccag acgaaaatga gatggcgcgc gactggcagc
    70561 tgatgttcat cagcgtgccg gttattttat tgcttgaact agtgtttgcg acgtggagct
    70621 ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc gcgcccgctg gccgcattct
    70681 tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg ggccgatgcc aacttctatc
    70741 gcccgatcac catgcagcgc gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt
    70801 ttcttgagaa gcatggtctg cttgatgcgc aggagtatca acgccgtctt attgagcaag
    70861 gtaatccaga cgccgtttcc gttcagtatc cgttaagcga actgcgctat cgcgatatgg
    70921 gcaccgggca gaatgtgctg ttgattactg tcgatggcct gaactactca cgcttcgaga
    70981 agcagatgcc tgcgctggca ggttttgctg agcaaaatat ttcgttcacg cgccatatga
    71041 gctccggcaa cactacagac aacggcatct ttggcctgtt ctatggtatc tcgccgagct
    71101 atatggacgg cattctgtcg acccgtacgc ctgcggcatt aattactgcg cttaatcagc
    71161 aaggctatca gctggggtta ttctcatcag atggctttac cagcccgctg tatcgccagg
    71221 cattgttgtc agatttctcg atgccgagcg tacgcaccca atccgacgag cagaccgcca
    71281 cgcagtggat caactggctg ggccactacg cacaagaaga taaccgctgg ttctcgtggg
    71341 tttctttcaa tggcactaac attgacgaca gcaatcagca ggcatttgca cggaaatata
    71401 gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt
    71461 ctggcaaact ggacaatacg gttgtgatta tcactgccgg tcggggtatt ccgctgagcg
    71521 aagacgaaga aacctttgac tggtcccacg gtcatctgca ggtgccatta gtgattcact
    71581 ggccaggcac gccggcgcag cgtattaatg cgctgactga tcataccgat ctgatgacga
    71641 cgctgatgca acgcctgcta catgtcagca cacctgccag cgaatattcg caaggtcagg
    71701 atttgttcaa ccctcaacgc cgtcattact gggttactgc agcggataac gatacgctgg
    71761 caattaccac cccgaaaaag acgctggtgc tgaacaataa cggtaaatac cgcacttaca
    71821 acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta ttgcaagtgc
    71881 tgacagacga gaagcgtttt atcgctaact gattaattat aaatcagtta gcgaaatatc
    71941 ttacttgcaa tcggtgtgga aaacggtagt attagcagcc acgagtcggc acgtagcgca
    72001 gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga
    72061 ccaaaaatcc caagaaaaaa ccaaccttta cggttggttt ttttatatct gcaattaatt
    72121 tgataaacag accgtgacac atcacagcct gtttattttc tgttatcaga acgtccagac
    72181 cactcccgcc tgagtattcc acggcgattc tacccctgcg ccattgccat agctgactga
    72241 caaatgaccg cttaacgtcg gggtaaacga tgaccttatc ccagcctgat aaacgccacg
    72301 ggttccagcc acatcattat taaatttgcc atcgtcatta actttcacat ggttagaatc
    72361 ggcgtattcc tgacgcacgg cggctttcag ccagggttcc aacgtcgtac cgttttgcag
    72421 gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt aatatccggg tatttcccac
    72481 atccgcgcgc atgccgtttg ataacgtgta gtcctgacca tctgtggtaa agccggtaaa
    72541 ggcgagatag ggtctaacac tccacaatcc gtcaacccaa cggaacccgc tctcaacatg
    72601 agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc
    72661 atggatggtg ttggcaaaac ggtcaacttt caccacccca tcaacatagg caccgttctg
    72721 atgctcccaa ccggcataag cccccagggt gtagctatca atattgcctt tgccgccgcg
    72781 atcaaaacca atatcagaat gagagtaatc aaagaacaag ccgcgaattg tgctgctttc
    72841 ttcacgggag aaacggctat cgataccgag cgtcaggccc gtcaatgttt gctcaaagcc
    72901 agctcccgca tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt
    72961 atcgtaatta acgcctttta cgctaccaag acgctcacgc acggtgtcca gttctgcatc
    73021 aaataccagc ggttgtgctg ctgccatgtt cagcacatca gtggttgaag gggtaatttg
    73081 cgcgcgattc tctgcgaggc tccagctatg gttgccatta tccagcaagg tatattcata
    73141 cgtaccgata tcaacaacgc ctccggcatt gcccaacgtg aatgcagcat caccgccgcc
    73201 cgtcgttacc agtgtaaggc tatctcctgc tgccgggctg gcccccgtgt ccgtcacgaa
    73261 tattttgaaa tcacctgttg cctgaccgat gacgttgagc tgatcgctct gatgattagc
    73321 catatcggta cgcatataaa attgccgttt cccgacaggg tattggtggt caacgtaatg
    73381 aaattttccg cacttgccgt cacagatgaa cgagtcactg gctcagccat cataatcaca
    73441 tttgcgccgg acatcgcaag gttggctaag ttgaggcgag tgttttggtt cgtcgtagtg
    73501 atatcggcaa tgatggtcca tgcgctattt tcgagcgaaa cgtccgcttg gctggtatcc
    73561 acggcaccat gcgttctaaa agaagcgccc tcagaaacca ctaccttacc caccaacata
    73621 gcgggtgcat aatttatttt cggcttcatg acctcaagaa tgccatcatt ccctctgact
    73681 gacacgttaa ccattgtgcc agcccagaca ttagcgcggc cattgttgat aaccatattt
    73741 tcagagatgg ctttagagga atacgtgata cttccattct gatctgatcg accgagatca
    73801 tatacagcat ttgcctgcac tttcgtacca gtatcctttc ccagcgattg catagtggca
    73861 tgctcatcaa ggatagtgtc aatggcctga gtgtcctcca taacaatgag cccggagcca
    73921 tcatccagtt cataattttt tgacacacca tcctttatac taaattggcc tttactgttt
    73981 gtaccactca cttccagcgc attcgttgag acaattaatt tcccgcctgc ttttttatca
    74041 acgccagttg cagtcccgcc atccataacc tccagtaatc ctcccttttc gatagcggca
    74101 aagacggcaa tcccatcttt ttcaacaatt aagcgcccgc ctttaatcgt gggatcattc
    74161 gccgc
//
LOCUS       DAJTVE010000004        69748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000004 DAJTVE010000000
VERSION     DAJTVE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..120)
                     /locus_tag="OO439_000237"
     CDS             complement(<1..120)
                     /locus_tag="OO439_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6314713.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMS"
     gene            complement(142..2154)
                     /gene="hyfR"
                     /locus_tag="OO439_000238"
     CDS             complement(142..2154)
                     /gene="hyfR"
                     /locus_tag="OO439_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6314714.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2184..2597)
                     /gene="hyfJ"
                     /locus_tag="OO439_000239"
     CDS             complement(2184..2597)
                     /gene="hyfJ"
                     /locus_tag="OO439_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6314715.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(2590..3348)
                     /gene="hyfI"
                     /locus_tag="OO439_000240"
     CDS             complement(2590..3348)
                     /gene="hyfI"
                     /locus_tag="OO439_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6314716.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3345..3884)
                     /gene="hyfH"
                     /locus_tag="OO439_000241"
     CDS             complement(3345..3884)
                     /gene="hyfH"
                     /locus_tag="OO439_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6314717.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(3894..5609)
                     /gene="hyfG"
                     /locus_tag="OO439_000242"
     CDS             complement(3894..5609)
                     /gene="hyfG"
                     /locus_tag="OO439_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6314718.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(5599..7179)
                     /gene="hyfF"
                     /locus_tag="OO439_000243"
     CDS             complement(5599..7179)
                     /gene="hyfF"
                     /locus_tag="OO439_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6314719.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7184..7834)
                     /gene="hyfE"
                     /locus_tag="OO439_000244"
     CDS             complement(7184..7834)
                     /gene="hyfE"
                     /locus_tag="OO439_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6314720.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(7846..9198)
                     /gene="hyfD"
                     /locus_tag="OO439_000245"
     CDS             complement(7846..9198)
                     /gene="hyfD"
                     /locus_tag="OO439_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6314721.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9174..9284)
                     /locus_tag="OO439_000246"
     CDS             complement(9174..9284)
                     /locus_tag="OO439_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6314722.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9301..10248)
                     /gene="hyfC"
                     /locus_tag="OO439_000247"
     CDS             complement(9301..10248)
                     /gene="hyfC"
                     /locus_tag="OO439_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6314723.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10259..12277)
                     /gene="hyfB"
                     /locus_tag="OO439_000248"
     CDS             complement(10259..12277)
                     /gene="hyfB"
                     /locus_tag="OO439_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6314724.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12277..12894)
                     /gene="hyfA"
                     /locus_tag="OO439_000249"
     CDS             complement(12277..12894)
                     /gene="hyfA"
                     /locus_tag="OO439_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6314725.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13147..13617)
                     /gene="bcp"
                     /locus_tag="OO439_000250"
     CDS             complement(13147..13617)
                     /gene="bcp"
                     /locus_tag="OO439_000250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6314726.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(13617..14189)
                     /locus_tag="OO439_000251"
     CDS             complement(13617..14189)
                     /locus_tag="OO439_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6314727.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14335..15213
                     /gene="dapA"
                     /locus_tag="OO439_000252"
     CDS             14335..15213
                     /gene="dapA"
                     /locus_tag="OO439_000252"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6314728.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15230..16264
                     /gene="bamC"
                     /locus_tag="OO439_000253"
     CDS             15230..16264
                     /gene="bamC"
                     /locus_tag="OO439_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6314729.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            16477..17190
                     /gene="purC"
                     /locus_tag="OO439_000254"
     CDS             16477..17190
                     /gene="purC"
                     /locus_tag="OO439_000254"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6314730.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17358..18206
                     /locus_tag="OO439_000255"
     CDS             17358..18206
                     /locus_tag="OO439_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6314731.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18221..20236
                     /gene="tmcA"
                     /locus_tag="OO439_000256"
     CDS             18221..20236
                     /gene="tmcA"
                     /locus_tag="OO439_000256"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6314732.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20310..21008
                     /gene="ypfH"
                     /locus_tag="OO439_000257"
     CDS             20310..21008
                     /gene="ypfH"
                     /locus_tag="OO439_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6314733.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21118..21318)
                     /locus_tag="OO439_000258"
     CDS             complement(21118..21318)
                     /locus_tag="OO439_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6314734.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21346..22473)
                     /gene="dapE"
                     /locus_tag="OO439_000259"
     CDS             complement(21346..22473)
                     /gene="dapE"
                     /locus_tag="OO439_000259"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6314735.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(22477..22833)
                     /locus_tag="OO439_000260"
     CDS             complement(22477..22833)
                     /locus_tag="OO439_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6314736.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23215..23274
                     /gene="ypfM"
                     /locus_tag="OO439_000261"
     CDS             23215..23274
                     /gene="ypfM"
                     /locus_tag="OO439_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6314737.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23373..26486)
                     /gene="acrD"
                     /locus_tag="OO439_000262"
     CDS             complement(23373..26486)
                     /gene="acrD"
                     /locus_tag="OO439_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6314738.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(26650..28350)
                     /gene="narQ"
                     /locus_tag="OO439_000263"
     CDS             complement(26650..28350)
                     /gene="narQ"
                     /locus_tag="OO439_000263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6314739.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            28556..30535
                     /gene="aegA"
                     /locus_tag="OO439_000264"
     CDS             28556..30535
                     /gene="aegA"
                     /locus_tag="OO439_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6314740.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            30603..31178
                     /gene="nudK"
                     /locus_tag="OO439_000265"
     CDS             30603..31178
                     /gene="nudK"
                     /locus_tag="OO439_000265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6314741.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31304..32347
                     /locus_tag="OO439_000266"
     CDS             31304..32347
                     /locus_tag="OO439_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6314742.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32443..34446)
                     /gene="tkt"
                     /locus_tag="OO439_000267"
     CDS             complement(32443..34446)
                     /gene="tkt"
                     /locus_tag="OO439_000267"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6314743.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(34466..35416)
                     /gene="tal"
                     /locus_tag="OO439_000268"
     CDS             complement(34466..35416)
                     /gene="tal"
                     /locus_tag="OO439_000268"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6314744.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35705..37984
                     /gene="maeB"
                     /locus_tag="OO439_000269"
     CDS             35705..37984
                     /gene="maeB"
                     /locus_tag="OO439_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6314745.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38277..38612
                     /gene="eutS"
                     /locus_tag="OO439_000270"
     CDS             38277..38612
                     /gene="eutS"
                     /locus_tag="OO439_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6314746.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            38625..39104
                     /gene="eutP"
                     /locus_tag="OO439_000271"
     CDS             38625..39104
                     /gene="eutP"
                     /locus_tag="OO439_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6314747.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39079..39780
                     /gene="eutQ"
                     /locus_tag="OO439_000272"
     CDS             39079..39780
                     /gene="eutQ"
                     /locus_tag="OO439_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6314748.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            39777..40580
                     /gene="eutT"
                     /locus_tag="OO439_000273"
     CDS             39777..40580
                     /gene="eutT"
                     /locus_tag="OO439_000273"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6314749.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            40577..41593
                     /gene="pta"
                     /locus_tag="OO439_000274"
     CDS             40577..41593
                     /gene="pta"
                     /locus_tag="OO439_000274"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6314750.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            41632..41925
                     /gene="eutM"
                     /locus_tag="OO439_000275"
     CDS             41632..41925
                     /gene="eutM"
                     /locus_tag="OO439_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6314751.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42032..42319
                     /gene="eutN"
                     /locus_tag="OO439_000276"
     CDS             42032..42319
                     /gene="eutN"
                     /locus_tag="OO439_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6314752.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42331..43734
                     /locus_tag="OO439_000277"
     CDS             42331..43734
                     /locus_tag="OO439_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6314753.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            43745..44581
                     /gene="eutJ"
                     /locus_tag="OO439_000278"
     CDS             43745..44581
                     /gene="eutJ"
                     /locus_tag="OO439_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6314754.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            44571..45758
                     /gene="eutG"
                     /locus_tag="OO439_000279"
     CDS             44571..45758
                     /gene="eutG"
                     /locus_tag="OO439_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6314755.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46075..47301
                     /gene="eutH"
                     /locus_tag="OO439_000280"
     CDS             46075..47301
                     /gene="eutH"
                     /locus_tag="OO439_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6314756.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47298..48701
                     /gene="eutA"
                     /locus_tag="OO439_000281"
     CDS             47298..48701
                     /gene="eutA"
                     /locus_tag="OO439_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6314757.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            48713..50074
                     /gene="eutB"
                     /locus_tag="OO439_000282"
                     /pseudo
     CDS             48713..50074
                     /gene="eutB"
                     /locus_tag="OO439_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50095..50982
                     /gene="eutC"
                     /locus_tag="OO439_000283"
     CDS             50095..50982
                     /gene="eutC"
                     /locus_tag="OO439_000283"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6314758.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            50992..51651
                     /gene="eutL"
                     /locus_tag="OO439_000284"
     CDS             50992..51651
                     /gene="eutL"
                     /locus_tag="OO439_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6314759.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            51664..52164
                     /gene="eutK"
                     /locus_tag="OO439_000285"
     CDS             51664..52164
                     /gene="eutK"
                     /locus_tag="OO439_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6314760.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52210..53262
                     /gene="eutR"
                     /locus_tag="OO439_000286"
     CDS             52210..53262
                     /gene="eutR"
                     /locus_tag="OO439_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6314761.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53268..54167)
                     /gene="hemF"
                     /locus_tag="OO439_000287"
     CDS             complement(53268..54167)
                     /gene="hemF"
                     /locus_tag="OO439_000287"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6314762.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54171..55040)
                     /gene="amiA"
                     /locus_tag="OO439_000288"
     CDS             complement(54171..55040)
                     /gene="amiA"
                     /locus_tag="OO439_000288"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6314763.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55254..55679
                     /locus_tag="OO439_000289"
     CDS             55254..55679
                     /locus_tag="OO439_000289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6314764.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            55666..56115
                     /locus_tag="OO439_000290"
     CDS             55666..56115
                     /locus_tag="OO439_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6314765.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56176..56751
                     /locus_tag="OO439_000291"
     CDS             56176..56751
                     /locus_tag="OO439_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6314766.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            56847..57746
                     /gene="yfeX"
                     /locus_tag="OO439_000292"
     CDS             56847..57746
                     /gene="yfeX"
                     /locus_tag="OO439_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6314767.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(57804..59108)
                     /gene="pbp4b"
                     /locus_tag="OO439_000293"
     CDS             complement(57804..59108)
                     /gene="pbp4b"
                     /locus_tag="OO439_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6314768.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59113..59286)
                     /locus_tag="OO439_000294"
     CDS             complement(59113..59286)
                     /locus_tag="OO439_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6314769.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59299..60537)
                     /gene="murP"
                     /locus_tag="OO439_000295"
                     /pseudo
     CDS             complement(59299..60537)
                     /gene="murP"
                     /locus_tag="OO439_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(60541..61437)
                     /gene="murQ"
                     /locus_tag="OO439_000296"
     CDS             complement(60541..61437)
                     /gene="murQ"
                     /locus_tag="OO439_000296"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6314770.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            61601..62458
                     /gene="murR"
                     /locus_tag="OO439_000297"
     CDS             61601..62458
                     /gene="murR"
                     /locus_tag="OO439_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6314771.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            62587..63378
                     /gene="ucpA"
                     /locus_tag="OO439_000298"
     CDS             62587..63378
                     /gene="ucpA"
                     /locus_tag="OO439_000298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6314772.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            63549..64565
                     /gene="cysP"
                     /locus_tag="OO439_000299"
     CDS             63549..64565
                     /gene="cysP"
                     /locus_tag="OO439_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6314773.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            64565..65398
                     /gene="cysT"
                     /locus_tag="OO439_000300"
     CDS             64565..65398
                     /gene="cysT"
                     /locus_tag="OO439_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6314774.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65398..66273
                     /gene="cysW"
                     /locus_tag="OO439_000301"
     CDS             65398..66273
                     /gene="cysW"
                     /locus_tag="OO439_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6314775.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66263..67360
                     /gene="cysA"
                     /locus_tag="OO439_000302"
     CDS             66263..67360
                     /gene="cysA"
                     /locus_tag="OO439_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6314776.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            67494..68405
                     /gene="cysM"
                     /locus_tag="OO439_000303"
     CDS             67494..68405
                     /gene="cysM"
                     /locus_tag="OO439_000303"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6314777.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68408..68776)
                     /locus_tag="OO439_000304"
     CDS             complement(68408..68776)
                     /locus_tag="OO439_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6314778.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            68881..69732
                     /gene="pdxK"
                     /locus_tag="OO439_000305"
                     /gene_synonym="thiJ"
     CDS             68881..69732
                     /gene="pdxK"
                     /locus_tag="OO439_000305"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6314779.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16518 a        18195 c        19311 g        15724 t
ORIGIN      
        1 ggacatcgcc attaaaaaga ccgacacttt gctgcgggca attttatggg cagtaatccg
       61 ttcagcgatt gccgcttttc tggcgctcaa ctgcaagtcg aaagagagtt tgtttctcat
      121 gcatactcct gcaaaagcag attacaacac ctcgcgaacc gagagcccca gacgctgcat
      181 tcgtgacagc agcgtggtgc gcttcatccc taatcgtgtc gccgcgccac ggggcccggc
      241 aacaatgcca ttggtttctc gcaatacctg aacaatgcgc tgacgttctt cttcgtcatt
      301 ctctggcgtc gtcgggtgca gcaactgcgc cattgaactg cgaagcgctg aatcttcatt
      361 tagcgtcggc agtaaatggc tttgtcggac attaagatgt aaattcagac tgttaccgcg
      421 agtcagcagt accgctcgct caatcacgtt ttccagctcg cgcacgttgc ccggccagtc
      481 ccacgacatc aactggcgta gcgcctcggt cgggatggcg tcaattgaac gattcatatg
      541 gcgcgccatt ttttgcgtga aatgctttgc taaaagaggg atatcttccg gacggtcgcg
      601 tagcggcggc aattccagtg ggaagacatt caggcgataa aagagatcgc tgcgaaactg
      661 gcgatcttca accatttgcc acaaatcacg gttggtggcg gcaatgactc tgacattcac
      721 cgggatcgtt ctactcccgc cgagacgctc aatctcccgc tcctgcaata cgcgcagcag
      781 tttaggctga agttctaacg gcagatcgcc aatttcatca agaaacaacg tgccgccatc
      841 ggcaatttca aaacgaccac gatgggtatt aatcgcacca gtaaacgccc ctttgtcatg
      901 accgaataac tcactttcca gaagactggc ggggatggca gcgcagttga ttttgaccag
      961 cggcttgtcg cgtcgcgggc taagttgatg gatcgctctg gcgatcacct ctttgccggt
     1021 tccggtttca ccacaaatca acactgtact gtcgctcttc gccacaatat ctacctgctg
     1081 gagcaggtct tccattgcct ggctttgata gataatgtca ccgacgccca gattcgaaag
     1141 gagctgctcg ctaagctggt ggttttcttg ctgcaaactt tcctgcaaat cggtcatgct
     1201 acgccaggca tcggcattgc caacggcaat agcgatgcga tcggctatgt gttgtagtag
     1261 ctgacagttt tcctcactaa agagagtgga agaggtatgc gccagcaaca atgcacccgg
     1321 tgtatggttg ccaaaggtaa gcggtataag gagcgcagat tcgcagccgt tagatgcaag
     1381 taagagtaat aacgggtcgc gttgccagag aaacagatcg tctgcacggt gcgtcaaggt
     1441 cggttgtcgg gtttgtagcg tttgtgtcag caatacactg tcgccaggca tattgcgtgg
     1501 cagacacgca caatgtgagg cagaaagatc gctgcaccac aggctaaact tctcgttctt
     1561 tcgatgatcg cccagtacca tactgactga agccagaccg aaaaaatgat ggatctcacg
     1621 agcgacgtca gcgatcagat cgtcgagatc aagatgtgac agcactgcat tagtaatatc
     1681 aaccagtacc cgatgatgat cgcgatcttt ctccagcaaa cgcagcgcca cctgctgttc
     1741 ttcatcattg agccatgatt gcagaaccgg ggtgattatc cgcgccagcc agttaaacag
     1801 tgtgcaatcc tggggagtaa aacggcagcc gtgggtgcga ataaagcgta gctccgccat
     1861 tccactactg ccgagaagga cggcgtgacg cgaataatcg accatctcgc cctgatactc
     1921 tgttgttgcc gtcacatcgc tgccatcatc atggtagcgg taatagaagt tgcttttgag
     1981 aacatgcagt tcaataagtt gtaccgggct gaacggcggc ggcagcggga tgaaggcgcg
     2041 taataaagac gccttaccgt gtttctgcgc taaccgctcc gcgagcattc cgtttaccgc
     2101 ttcaattgtt attccttgcg gcggggcaaa catcgcctcg tctgacatag ccatcaacag
     2161 tccttattaa tgagaaatcc ttctcacgca atttttctgg ccatcagata gatggccggt
     2221 tcctgcacaa tttcatccaa cagatgaatg aattgaatgc tacgcgcctg ccacacagac
     2281 gtcgcgtcat ccgccagtgg cgcgaatgcc cgggtcaaca gggcggtgtg gctggcgtca
     2341 ataacaatct caccaaaagt catcaccccc agcatcttac gtcgggcttg ctcctcttcg
     2401 accagtgcaa gccaatcttc gtattccgtc agtgggcagg ggaaggcgac attcagacaa
     2461 tcaatcacgc caacgtgatg accgatagcc agcgagtaat acattacctg agagctgtgt
     2521 tccggcatct cgtcgctact ggcgacaaac ttttttcgca gcgtccagaa aacaatttcc
     2581 ccgcactctt cagtcatgta acccccggag tacgcgaaag agttgctgaa cgatactatt
     2641 gagacgtgga tcgtcggcat cgcgcaacca ggtgttaacc cgatttcctg taggatcgtc
     2701 gcttaaatgg cgcaggagcc gatcgcaaat ttctcgcccc tgacgatagc ccgccagccg
     2761 ccgcgcttct cgctcaatgg cgatacgctg tgatggcggg atctgcggcc acaacggttg
     2821 catagtcacc ccggtgggat cgcgataatc caccgcgtga atcttctgtt gcagcaaacc
     2881 gagcgccacg gcgaaaccgt gaatggtggc ggccggtgtt ggcgggcagc cggggatcca
     2941 aacatcaatg gggacaatgg tgtcgctgcc gccccagacg ctgtagagat cgtggaaaat
     3001 accgccgccg acaccgcacg cgccgtagga aacacaaatt ttatgatcgg gggcagactc
     3061 atacgcccga agtgcaggca tacgcatcgc ccgggtgact gcgcctgtaa ataacaaaat
     3121 atcggcgtga cgcggtgatg aaataacctt aatgccaaaa cgttctgcgt cgaatactgg
     3181 tgtgatggcg gcaaaaattt cgatttcaca ggcgttgcag ccgccgcaat cgacgcgata
     3241 aacgtaagcc gagcgacgga tatcctgtag caaatgctgc ttcatctttt gcgtttgctc
     3301 gtccagcgtg atgggctggc tgacatgttg tgtaagcact ggactcatag ctgctcctta
     3361 gccaccagcg gtacatctgt atcgtcgttg agcagcgtcg cgcgttgttt gcactccggg
     3421 cagacgctcg cttgcgccca taacatttcg cggttttgtg gcgcattttg ttgctgtgcc
     3481 aacaattcag cagccagtgc gacggttttt tgcggggcaa acgggcgttc gcaacggctg
     3541 caacgttgta gatggaacgt cgcgcgggta tagagatcgg ctttattggt gacggtcagt
     3601 tcaaagttat tggtaagctg gatggctctg gtcgggcaca cttcttcaca acgtccgcag
     3661 taaatacaac gcctcagata gagctgccag gtgcgcgaat tttgctggtc gtcggtctgg
     3721 atagtcagcg catttgccgg acaagcacag gcgccgcagg caatacattg gctgggcatc
     3781 aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc
     3841 gtcgcggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc
     3901 agcggcgaac ggttacgatc aatgccgtag cgttcgatct ctttatacgg cacggttttt
     3961 gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca
     4021 aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac
     4081 accggccagt tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt ttggttatca
     4141 ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat
     4201 gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg
     4261 gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag
     4321 aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag
     4381 gggtgatcaa agcgcaaatc acgggcaaaa ccactgccgc ggatcagcgg ccctacaggg
     4441 ctataatcac gggcgatttg tcggtcgaga atgccaatgc cctgagtgcg ttgttccata
     4501 ttcggcgtgg caagcagcat ctctaccagc tccgacacgt cggcgcgcat ctcgcgcacc
     4561 agtttcagcg tttgcagacg ttgctctttg agaatatcgc ggcgaacacc accaatcaga
     4621 ttcagaccgt aggttttacg cgacccggtc agcaattccg ccattgtcat cgacttttcc
     4681 cgcacgcgga aaaattgcat aaagccggta tcaaatccaa caaaatggca ggagaggcca
     4741 aggttgagca aatgactgtg cagacgttcg acttccagca gaatcgagcg aatagtatgc
     4801 gctcgttgcg gcacctcaat ccccagtgca ttttcaaccg agttggtata ggccacactg
     4861 tgggcaaaac cgcaaatccc acacacgcga tccgataaga aggtcacttc gttgtagccc
     4921 atccgcgttt ctgccagttt ctccatgccg cgatggacat aaaacaggcg gtaatcagca
     4981 tcgacaattt gctcgccatc cacaaacaag cggaagtgac ccggttcatc ggaggtgata
     5041 tgcagcgggc caatagggat cacccgcgca tcgctattgc cctcattgat aaacggatac
     5101 gtttcggtat cagtcgtcgg ttcagggcgc agtcgataat ccatcgcatc tttgcgcagc
     5161 ggatgcatat cttccggcca gttatcgggc aacaccaggc gacgctgatc cggcaggcca
     5221 accggaatca gcccgtacat atcgcgaatt tctcgctcgc cccagaccgc ggcagggacg
     5281 cgcggtgtga ctgacggaaa ctcccgactg tcggcatcga ccagtgcctt caccacgatc
     5341 cagcattttt cggccccttc cattgaaagg gcataataaa ccgcgtaatg accgttaagt
     5401 gtccgctcgt cgttgccaaa caggactgga agccagccat catgttgata ataaagataa
     5461 tgtacaacgt caggcagcag attgattttc accgtaatgg tgacctgttc aggcgtttgt
     5521 cgctcttcat ccagcaccgc gccggggaac tgcgttttca gggcggcgag aatcgcttcg
     5581 ccacgatttg acgatgaatt aacgttcact gtgtttctcc gaaacagatg cggtgcctga
     5641 aggcaaaaaa tcatgccagg tgctacgttg tgcaggcagg tcgtgcgtcc ctgagaggac
     5701 tatagtggaa gcgcccgcca ggatcctgat gacaggttgt ggaatatgcg ttcccatcgc
     5761 cagcatcatg accagcagaa tcaccattgg cgaggtggtc aaccagccga gatcaccccg
     5821 gttaacggcc tgcggcggtt tcgccattaa cacccgcgct gccatccgta ccaggcccgc
     5881 cagcaccagc gttaacagca ataacagcag gacgataatc agcaggtgat tacgtgccag
     5941 tccggcggta atggtcataa attcgctaag aaaaatgttg aagggcggca tccctgccag
     6001 cgccagcgca ccgccgccaa acagcacggc ggtaaatggc atgattttga gcatcccaca
     6061 gacgacgttg agatcgcgcg tgccgtactt gagcagtaca ttgccggaac cgcagaacag
     6121 cagcgttttt gccagactgt ggtttaagat gtgcagcagc gcggcaaaaa ttcccagcgg
     6181 cccgccaatg cctagcgcca ccgcgaccag ccccatgttc tccacgctgg agtacgccag
     6241 cagacgctta atgtcccgct gtaccagaat gaaaaatgcc gccacggcaa ccgacaacat
     6301 gccgaagatg agcaacaacc ggttggggaa atcgctgccg atggcttggc aaataatgat
     6361 gtaatagcga atcagcacca acagcgcgca gttcagcaat acggcggaga gcagggcgct
     6421 gaccggactc ggcgcttcac tgtgagcatc cggcagccag gcgtgcatgg gaaatagccc
     6481 ggttttggta ccaaagccaa ttagcacaaa cacaaaggcc aacagcatta atgttgggtc
     6541 aagcaaggac gattgcttaa gaacctcgct ccagaatatc gccatttctg cctgcggcat
     6601 aacgctggcg gcgttggcgt ataccagcac ggtaccgaac agaccaaaag cgacaccaac
     6661 agtacaaata atgatgtact tccatgctga ttccagcgat gaacgctgac cgtaaatgcc
     6721 taccagaaac gccgagctta aggtggtggc ttcgatcgcc gcccacatca caatcaggtt
     6781 attgctggta acaaccagca gcatggtgaa caaaaacaga tggaagaaac cgtagtaatc
     6841 gcacagcgta acgggtgaaa gctcgccgtg tgccacttca tgacgcatgt agccaatcga
     6901 gtaaacaccg gtgagaaagc caatcacacc aagaatggcg aggaacaaac cgcccagacc
     6961 atcaatatga agccacagtc ccgcagcgaa tatttctcct gcatcagcgg cagtttggac
     7021 cacccagagt gccagaatca gcagcagtgt gatccctaag ctatgtaata cagtcaccgt
     7081 acaagtcgca gagagccccc gtttccggca ggcaaaacag agcagcgaaa aaagcagcgg
     7141 cgtgagcagg agtaaagcga acatcacaga ataactcatc tcattatccc ttcagcgcgg
     7201 tcaagttgtt cacgtccagc gtgccgtggg tacgccatat ttttcttgcc agtaacacca
     7261 tcacaatgac ggcgaagatg gcgtcggtag cgatacctat ttccaccagt tccggtgctc
     7321 gccaggcaag aagcgccagc accagatggg agccgttttc catcaggcag taaccaaaaa
     7381 tttgccgcag gatattgcgc tggctgacaa tgcacagcag gccaagcaga aaatgaccta
     7441 acgctaccgc cagcgccggt ttcagcccgg tagccatcgg tagcttcacg ggctgaacga
     7501 caaatgcgca aagcaggaca attaacgccg cgagcagtgc catcattgcc ggaccgaata
     7561 acgctttttc cgggatgttc tggggaatat ttcgtgcagc gtaagtcatg attaacggta
     7621 ccagcagcac tttggtgata aaggcgctgg cggaccagat cagcagttgc tctgcggcga
     7681 acaggcacga gagagtggcg aaaatagaca ccagcaccag cgactggcag gcgtaaaatc
     7741 cgcatgacag gcgatagctt ttgacgctaa tcacaaacag cgatgtcagc atcatcagtc
     7801 ccgccagatt atttacgatc atagaaccgg tcattcatca tctccttact gcaaccaggt
     7861 cgcggcgatt acgctggaaa tcagcgacat cacaatcagt acaatcaaca ccaggcgcat
     7921 tgaccctggc agcggtgcgg catcggcgac ggcctcgctc ggtttgcccg gcacgacgcg
     7981 accaaaccag cgaataaacc aggcgaaact ggcgaccgat tcaatcatca gcagaatcat
     8041 ggcgggcagc aggatccagt actccactga caacgcaaaa ccgacggcaa acagcgggaa
     8101 tttactgaag aagccgttga acggcggcac accggtaatc gccagcgctg ccacgcagaa
     8161 acccacgctt ggcaacggca gggtgtgcaa tacgccacgc agacgcggca acaagcgcgt
     8221 gccgcagctg taactcagcg cacctgctac aaggaaaaac aggcttttag cgaacgcgtg
     8281 gttgacgatg taggcgatgc taccctccag cgccagccgc gagccgaaga tggagagcga
     8341 caagccgaag aacatccagc caagttgagt gatggtcgac caggccagca accgcttcat
     8401 atcctgctgt ggcaaataca tcagaaagcc ataaagaatg gtgaccagcg ccatgaccat
     8461 gccaacgccg ccaatcacat gcgggatatt gccgccgtcg ataatggcgc gggcaaaaat
     8521 gtaaacgccc actttcacca tcgatgcggc gtggagatag gcactgatcg gtgttggcgc
     8581 ttccattgca tccggtagcc acgcttgcat cggtagctgg gccgatttcc cccacgcggc
     8641 aaacaggatg ccgccataaa ccagataacg tgcgtcgccg tgtaactcgc tcatcgcgct
     8701 aagcgcaaac gttccggtct gcaaaaacag cgtggcggcg gcaagataca accccaacga
     8761 gccgatatga gtgataagta acgcttttag tgctgaaagc tgtgctttat cgctctggta
     8821 ataactgatc aacgcccagg agcagccgcc cgtaatttca aaaaacaaca actgaccgag
     8881 cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca aaaatgcgta
     8941 ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat aacccgtgga
     9001 gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg tactcatgcg
     9061 atcaatgacc aagccaaaca atgccacatc ccccacgttg accaacgtca acgtgacggc
     9121 aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg tggtcagtgc
     9181 ggcgaacaaa acccccattc ggcggcccga cgttgtggcg aaaacgaaac gaccagtgcg
     9241 ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg tcagtgctcc
     9301 ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc caagccaggt
     9361 cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga ttgaggccag
     9421 tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca ggcaggcgag
     9481 agaaagttct tgcgcgcgcc caaagggcag aaacagggcc acaaacagtg ctgccatcac
     9541 gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg aatattcggt
     9601 cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa tttttcccat
     9661 ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta gcggcgagtt
     9721 ccagcccgtt gccagcgtat tgctgatcat ctcgatatgc gtggaacctg ctatcagcgc
     9781 caataccagc agtgagagaa tgagcatggg ttcgaccaga atgccgagcg tcaactcgcg
     9841 actggcaccg actcccgcaa acgggcttcc ggtatccagc cctgaaagag caaagaaaaa
     9901 acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg caaaagggga
     9961 aacggtaata aatagtggta gggccatcgc cagcaccagc atgctgctga ttaataccca
    10021 cggcatcagg cggaacatca gacctgaaga tgtcggcgca tcttcctggc gtttaaacag
    10081 tttgtggata tcgcgataat cctgccagat ccccggcccg cggcgggagt gcatacgcgc
    10141 gcgtatctgc cgggaaatac ccgtaaaaag tggggttagc atcagcagaa tcacggcctg
    10201 tgctaacgca aaaatgacca gatatccgtc gcaaagagtt tgtctcatgg tgatttcctt
    10261 agacggcaat agcgattagc agcacaacca gtgcggcgac cacatacagg caatagagac
    10321 gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg gcgctcacga
    10381 tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg gcggtgacgt
    10441 gcgcaagacc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga cgaaatagcg
    10501 cagaaaagac cacacgcagc ggctgcatca cgccattgcc tgacggggcc atcgcatttt
    10561 cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg cggcgcgaac
    10621 gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac agcaacagta
    10681 atgaaggagt gacccgtgta tgaaacgtgc cgggtacaag tgctattccg ctggcgacag
    10741 tgacgggagg ggtattggta aacgcatggg caatgtgcat tatcttcggt gccagccaac
    10801 tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg acgatcatcg
    10861 tatttggcac ttcctgtgcc tcttcagcgt gtgtgctgcg cggcgcacca cagaaagtaa
    10921 taccgtacat tttgacgaag cacattactg ccagcccacc agtgactgcc agcattacca
    10981 tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt gagaatagcg
    11041 actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct gatatcgcca
    11101 ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt gcccccattt
    11161 tttccatgtc gtgggtatgc aaacgcgaaa taatcgcacc cgcgccgaga aatagcagcc
    11221 ctttgaaaag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca accacggtga
    11281 gcagcgggtc gtgcagtgac agaccgacca tcgccacacc gaccgccagc aaaataatgc
    11341 cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc gccagtgcat
    11401 acagcacgcc caggagcgcg gagattgcgc cgatcgccat caccagaatg ccccaccaca
    11461 gcggcaaacc cgtttgcgcc agcagatcca tcgccacttt caggatgccg aaaataccga
    11521 ttttgaccat tacgccagac atcaacgctg aagcgtgcga tggtgcggca gggtgagcgc
    11581 gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg aaaaaggcca
    11641 gcaggaacac cgccgacgcc agccccggag aaagtgaaag cgtgcggaaa ctggcaaaat
    11701 cgaggctgcc gctttcgcgc cccatcagca agaaggcgat cattatcagc accgatccgg
    11761 cgtgggcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc tgcctggcaa
    11821 tgaccagaaa ccaggaagac agcgacatca tttcaaacag cacgatgaac caaaaagcgt
    11881 tgtccatcac cagcagggca accatcgatg cgatgaaaat attcataaag aagcccatcg
    11941 ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagaga gaacaaacca
    12001 ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt cgcagggtta
    12061 aatcggcaaa ttcaaaaggc gtggcaaaac gggcgactaa agttacgcct gcatgtaatt
    12121 gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg ccggaaagct
    12181 taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca aacagataga
    12241 ggatcagcga ccaggttaat aattgcaggg catccattag cgttgctcct gagtgaggga
    12301 aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt tttctttcat
    12361 ctggcgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc aagcgcgaac
    12421 gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga cggtctggac
    12481 acccggttcc cagcgcagca acgcatgttg gggcggcacg ttttcttcgc cgtctttgag
    12541 tgagccctca gcctgaaaaa catattgcgc aggggcattc accggacggc ttcctgaagc
    12601 gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac agaggctttc
    12661 gttgagttgg atcgcatcat ccctctgaga gatggcattg accgggcaga cctgcaggca
    12721 aggggcttcc tcacagtgat gacacacgac aggggcagtg agtgttgacg tcttcgccag
    12781 ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag
    12841 acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc
    12901 tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgct attctgatta
    12961 actcaatata ttcaataagt tatgtattaa ctggattctg aacggtacaa aaatgatggt
    13021 cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg
    13081 atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg gaatggagca
    13141 aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt
    13201 gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg
    13261 aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca
    13321 cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg
    13381 ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa
    13441 ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt
    13501 cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc
    13561 gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac
    13621 ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca
    13681 ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg
    13741 gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgc
    13801 gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt
    13861 tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc tggcattggc
    13921 ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagctc ggcacctttc
    13981 aacggtaacg ttgattcaat cagagtaatg gcattccatg aaccggaaag cagcatgata
    14041 aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta
    14101 ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa tgcagtgatc
    14161 accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc
    14221 ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt
    14281 ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc
    14341 acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct
    14401 agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt
    14461 ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg
    14521 acgctggagc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc taacgctact
    14581 gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg ctgcctgacg
    14641 gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc
    14701 gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat
    14761 ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag
    14821 gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt
    14881 ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattaggcgg tcatggggtt
    14941 atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca
    15001 gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa
    15061 ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg
    15121 gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc
    15181 agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc
    15241 tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat tactcgctgc
    15301 ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc
    15361 ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga
    15421 ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc
    15481 accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg gcgataccgc
    15541 ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct
    15601 gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccaccga
    15661 ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc
    15721 tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgttgaacc tggaacaggc
    15781 gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt
    15841 tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc aaaatcgtgc
    15901 ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccaa tgctggtcgt
    15961 acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat
    16021 gaaagtgacc gacagcaccc gttcgcaggg caacatggcc gtaacttata agccgctgtc
    16081 tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa
    16141 actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca
    16201 tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa
    16261 gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg caaacgtgtg
    16321 cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg tcggacgtag
    16381 ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga
    16441 ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat
    16501 cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc
    16561 cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg
    16621 ttgaacaaca agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact
    16681 caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg
    16741 gttgagtgtg tcgtgcgtaa ccgtgctgcg ggctctctgg tgaaacgtct tggaatcgaa
    16801 gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac
    16861 gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg
    16921 gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact gttcgatgat
    16981 gctggtctga ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta
    17041 ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa
    17101 atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc
    17161 gcccgccgcc tgggtgtaca gctggactga tttttctgtt catcatcttg ccgtgcagct
    17221 ggcacggcaa gacaaccgct ctcggaatat atgcattgaa cgctcattcc tccgccattt
    17281 catcccgctt tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat
    17341 atattgttga ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gtcgaagaca
    17401 ggcgtaacag ctccggcggt ccgtcaatgg gcggtcccgg ttttcgcctg ccaagcggta
    17461 aaggcgggct gattttactg atagtcgtgc tggttgcagg ctactatggt gttgatttaa
    17521 ccgggttgat gaccggacag ccggtttccc aacagcaatc aacacggtca attagcccaa
    17581 atgaagacga agccgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg
    17641 gacagcaatt cgagaagatg ggtaaaacct atcagcaacc gaagctggtc atgtatcgtg
    17701 gaatgacgcg taccggctgc ggggcgggcc agtccattat gggaccgttc tattgcccgg
    17761 cggatggcac ggtttatatc gatctctcct tctatgatga catgaaagac aaactcggcg
    17821 cggatggcga ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga
    17881 aattgttagg catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag
    17941 taaaccgctt atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc
    18001 ataacatgca gcagcaaggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg
    18061 atgaccgttt acaacagcaa agtcaggggc gagtagtacc agacagtttc actcatggta
    18121 cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggcgat ccggcacaat
    18181 gcaatacttt tggtaaaagc atttaacttc cggggcaagg atggctgaac tgactgcgct
    18241 tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg
    18301 ggaagagggt tggtgttttg atcatgcgct taagttgcgt gatgccttac ctggcgactg
    18361 gctgtggatt tcgccgcagc cagatgctga aaaccactgt tctccctcgg cgctacaaac
    18421 tttacttggg cgcgagttcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc
    18481 tgcctttgcg gcacttagcg gaacgttgaa agcgggaagc tggctggttt tgttactccc
    18541 tgtatgggaa gagtgggaaa accaacctga tgccgactcg ctgcgctgga gtgattgccc
    18601 tgaccctatt gcgacgccgc attttgtcca gcatctcaaa cgcgtactta cggcggataa
    18661 cgacgctatt ctctggcggc aaaaccagcc gttctcgttg gcgcatttta ccccccgtac
    18721 tgactggcac cccgcgaccg gcgcaccaca accagaacaa cagcaactct tacagcagct
    18781 actgaccatg ccgccgggcg tggcagcggt aacggctgcg cgtgggcgcg gtaagtccgc
    18841 gctggcaggg caactcattt ctcgtattgc gggcagtgcg attgtcaccg cgcccgcaaa
    18901 agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcgccgga
    18961 tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgatgaag ccgcagccat
    19021 acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac
    19081 ggtgcagggc tacgaaggta ccggacgtgg ttttttgctg aaattttgcg ctcgctttcc
    19141 gcatttacac cgttttgaac tgcaacagcc gatccgctgg gcacagggat gcccgctgga
    19201 aaaaatggtt agtgaggcac tggtttttaa cgatgaaaac ttcacccata caccacaagg
    19261 caatatcgtc atttccgcat ttgaacaaac gttatggcga agcgatccag aaacgccgtt
    19321 aaaggtttat cagttattgt ctggtgcgca ctaccggacc tcgccgctgg atttacgccg
    19381 gatgatggat gcaccagggc aacatttttt acaggcggct ggcgaaaacg agattgccgg
    19441 ggcgttgtgg ctggtggatg agggcggatt atctcaagaa ctcagtcagg cggtatgggc
    19501 aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggcagcaa
    19561 tccactggcg gcgacattgc gcggacggcg ggtcagccgg attgcagtcc atccggcgcg
    19621 tcagcgcgaa ggcgttgggc aacagctcat tgccagcgct ttgcaatata ggcctggcct
    19681 cgactatctt tcggtgagtt ttggttacac cggggagtta tggcgtttct ggcaacgctg
    19741 cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt gctatacggc
    19801 gatggcactg ttaccgatga gtgatgcggg taaacagctg gctgaacgtg agcattaccg
    19861 tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc gaaacgcttc ctgttgatcc
    19921 actaaacaat gccgtccttt ctgacgacga ctggcttgaa ctggccggtt ttgctttcgc
    19981 tcatcgtccg ctattaacgt cgttaggttg cttattgcgt ctgctacaaa ccagtgaact
    20041 ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacacc agtgacgcgc agttatgtac
    20101 cacacttaaa ctttcaggcc gcaagatgtt actggttcgc cagcgggaag aggccgcgca
    20161 ggcgctgttc gcacttaatg aggttcgcac tgagcgtctg cgcgatcgca taacgcaatg
    20221 gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa
    20281 aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc
    20341 cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg
    20401 caatggggga aataggcagc tggtttgcgc cgctatttcc tgatgcgctg gtggtgagtg
    20461 tcggaggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttcg gtgcagggta
    20521 ttacggaaga taatcgccag gcgcgtgtgg atgccatcat gccgacgttt attgagatgg
    20581 tgcgctactg gcagaaacag agcggagtag gggctaacgc cacagcgcta atcggtttct
    20641 ctcaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt gcttcacgcg
    20701 tcattgcttt taacggacgc tatgcgagcc tgccggaaac tgcgtctaca gccacgacaa
    20761 ttcacctgat tcatggcggt gaagatccgg ttatcgatct ggcgcatgcg gttgcggcgc
    20821 aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc
    20881 atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat accattccga
    20941 aacattattt cgacgaagcg ctaagtggcg gtaagcctgg cgatgatgat gtgattgaaa
    21001 tgatgtaaaa gacagattac ctggcctgat aagacgcgtg agcgtcgcat caggcatcgt
    21061 tcaccattgc cggatgcggc gttaacgcct tatccggcct acggttgagc actcagctta
    21121 cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc
    21181 acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttgtggtcga cgcgcttgag
    21241 atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata
    21301 tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcagg cgacgagctg
    21361 ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc
    21421 attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg
    21481 agcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt
    21541 atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg
    21601 ctgcccggaa agccaccaat ccaccgtata gcgcagttga tgtttttcaa gcagggcaag
    21661 cacctgcgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac
    21721 aaacagttca cccggaataa cgttgttact gcccgttccc gcctgaatat tggcaatctg
    21781 catactggtc gccgggaaga attcattgcc ctgatcccac tcaatagcca ctaattcatt
    21841 aaggaaaggt gctgcgcgat gtactggatt gtcagccaga tgtgggtagg ccacatgccc
    21901 ctgcacgcca tgaatggtaa ggttgcaggt taacgatccg cgacgaccat ttttcaccac
    21961 atcaccaacc acttcgatac tcgacggttc gccgaccaga cagtaatcga gacgctcatt
    22021 acgcgccatt aacgcttcga cgacttttac cgtaccgttg tgggcactgg cttcttcatc
    22081 agaggtgatc agaaatgcca gtcgtcccgt atggttggga tgttgtgcga caaaacgttc
    22141 tgctgctacc accatcgccg ccagcgagcc tttcatatct gccgcaccgc gcccgaataa
    22201 catgccatca cgaatggtgg gttcaaacgg cggattgatc caacgatcgg cgtcgccagg
    22261 cggcaccacg tcggtatgcc cggcaaaggc caatgtttca ccttgcccac gccatgccca
    22321 aaaattctgc gtatcggcaa agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg
    22381 atcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag
    22441 ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg
    22501 ataactggaa tcactgaaac ccagcagcat aggcttaccg ggcgcgcaga gcaatggacg
    22561 tttgataatt gcaggcattt cagtcattaa tgccgccgct gaggccgcat cggtgatttt
    22621 attgcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttga gtaacgcctc
    22681 ccaacctaat tcgttgataa aaccgttcaa taattcgctg tccagcccat cgacgcggta
    22741 atcatgaaaa cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt
    22801 gtcacaattt ttgataccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa
    22861 taaatactca tttaagggtg aaaacatttt gcctgcaaat gattatcagg aaatataaca
    22921 ttatcgaagt attcaaatta acaactatta aataatatta aacattttta acgtaaacat
    22981 taagaatatt catcttgtta tctgtctgta ttttaacctg aattaaatca gtattagatg
    23041 ccgtatgaat atctcgaaat gttgctgtgt atcacataca aatcatcacc gcaggaccat
    23101 agtatttaca taagatttgt ccccccggag gtagtcatcg cagcaatcga gtaaattagc
    23161 ttcaccacgg tatattgttt ccgtagaata cccataatta taagagaggt tgttatgatt
    23221 gaacgtgaac tggggaactg gaaagacttt atcgaagtta tgcttcgtaa gtaattcctg
    23281 agacaaagta taaacagcaa gaacccgctg agcaggttct taatcggaaa gttgaaaaag
    23341 gcgacacatt ggcatgtcgc cttttttatt gcttattccg ggcgcggctt tagcgggaag
    23401 cggcggcgca ccagcacaaa gaacagcggc acgaagtaaa tagccagaat agtggctgaa
    23461 atcatcccgc ccattacgcc agtacccacc gcatgctgac caccggaacc ggcacccgtg
    23521 ctggttgcca ttggcaatac gccgaagata aatgccagcg aggtcatcag aatcgggcgt
    23581 aaacgctgac ggcaggcgtg gagcgtcgct tcaaacaggt cgtggccttt ttggttcatc
    23641 tcgttagcaa actcgacgat caggatggcg tttttcgccg ataaaccaat gaccgttaac
    23701 aggcccacct ggaagtaaac gtcgttttcc agcccgcgca tccaggttgc cagcagcgcg
    23761 ccgattaccc ccagcggcac gaccagcatt accgagaacg gcaccgacca gctttcatac
    23821 aacgcagcca gacacaggaa taccaccagc aaggaaatgg cgtacagcgc cggagcctgc
    23881 gcgccggaaa gtcgctcctg atacgacatc gccgtccact ccagaccaaa gccgtttggc
    23941 agctgcttca ctaacgattc cataatatcc atcgccgtac cggtactgac ccccggtgcg
    24001 gcttccccaa caatctcaac cgcagaataa ccgttatagc gttccagacg cggcgagcct
    24061 gtttcccagc gtgaggtcgc gaaagcagag aagggcacca tgccgccatc tttatttcgg
    24121 acataccaga gattgatgtc atctggcagc atgcgatacg gcgcagctgc ctgcacatag
    24181 actttcttca cgcgaccgcg atccataaag tcattcacat agctcgaacc ccaggcggtt
    24241 tgcagtgtgt cgttaatatc gtcgatagca acgcccagcg cctgagcttt acgctggtcg
    24301 atatcaatct gcaactgcgg actgtcgtcg aggccgttat ggcgcacacg ggttagctcc
    24361 gggttttccg ccgccagcgc cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct
    24421 gctccagcgt ggtcctgcaa ctccatatca aaacccgcag aactaccaag accgctaatt
    24481 gctggcgggc tgctggcgat aacgcgagct tctttaatct tgttaaacgc ctttgttgca
    24541 cgctcgataa tggcaaacga ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg
    24601 cggataaaca ttcgcgccac gttttgcccg ttacccccag ggccagaacc aacggtggca
    24661 aacaccgaca tgatgttgtc tttttcatgg gtgaagtaat atttctcgat ttgctcaacg
    24721 actttcaggg tctgttgttg ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg
    24781 ccacggtctt ccagcggtaa gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg
    24841 ccaagcagca ggacataaat cacaatccag cgcaggctgc ggtggagaat tttcgccacc
    24901 cctttttcgt agcgttcggc gttgcggtta aacatctggt taaaccaggc aaaaaagcct
    24961 ttttgcccgt gatgctcacc ttttttcagt ggcttaagta gtgtggcaca cagagccgga
    25021 gtgaggatca tcgctaccag tactgacagc accatcgccg caacaatggt aatagagaac
    25081 tggcgataga tggcaccggt ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa
    25141 agcaccatcg caatcccgac cagtgccccc tggatctgcc ccatcgattt acgtgtggct
    25201 tcgcgaggag tgagtccttc ctcactcata atacgttcga cgttttccac caccacgatt
    25261 gcgtcatcca ccagcagacc gatcgccagt accatcgcga acatggttaa ggtgttgacg
    25321 ctgtaaccga aggcgtaaag tacggagaag gttcccatca acaccaccgg cacggcgata
    25381 gtgggtatca gcgtggcgcg gaagttttgc aggaacagat acataacgag gaaaaccagg
    25441 gcgatagctt ccagcagcgt tttcaccacg tcttcaatgg aggctttaac aaacgaggtg
    25501 gtttcatacg ccaccttgta ttccagtccg tgcgggaagt actgcgccag ctcgtcgaga
    25561 cgattgagca ccagctccgc tgtcgccatt tcgtttgcgc cggaggccag ttttaccccc
    25621 agcccggagg ctggcttacc attgaagcgg ctaagataat cgtatttctc cgcccccatt
    25681 tcgacggtgg cgacatcgcc cagccttacc tctgagccgt cttgattcac ccgcaaggtg
    25741 atatcgcgga actgttctgg tgtttgcagc agtgactggg cgttaatggt ggcgttgagc
    25801 gcctgcttat cgacggaagg tgtaccacca agttgcccaa ccgcaatctg cgcgttctgt
    25861 gactcaatgg catcagtgac atctttagcc gtcatctgga aactgttgag tttcgccggg
    25921 tccagccaga tacgcatgga atattgcgaa ccataggcat cgatatcccc gacgccattc
    25981 acgcggctta acgggtcctg aatattactg gcaacataat cggcaatatc ctgtttatcc
    26041 atcgaaccat cggtagagac gaaagcaatg gtcagaatat tggtatcgcc ggttttacgc
    26101 accgtcacgc cctgattttg caccgcctgc ggtaactttc gcatggctga ttgcagctgg
    26161 ttttgtactt gctgcacggc ttcgtccgga tcggtgcctg ctttaaaact taaagtgaca
    26221 gatgcctgac cgatgccact gctctgagat gacatataca tgagattatc gaggccggtc
    26281 atattttgct cgataacctg ggtcacggtg ttttccagcg tctgggccga tgcgccggga
    26341 tagttagcgg tcactcgcac attcggtggc gcgagatcgg ggtattgttc aacgggcaat
    26401 gaaaaaatcg ccagggtccc tgtcagacac aacaggattg ccagcaccca ggcaaaaatg
    26461 gggcgatcaa taaagaaatt cgccattaaa agaggacctc gtgtttctgc gtatcgttgt
    26521 aggcttgact tgcttcactg tagcggcaag gtacgagcca atcgtggaga aaaaaaggag
    26581 ttaatgtaaa ttatcatgcc cttttatact gaaccttaag tgcaagtatt ctttggtcag
    26641 taggaggcat tacatcaact gactttcctc accctccgca gagcgaaagc taatactcac
    26701 taacgtgccg ccgccggaag gttgtgaaaa agtcagcgtc ccacccagcc gttccgcgcg
    26761 ttcgcgcatg atattcagac cataatgacc ttcgggttct ttcggttcac cgataccaat
    26821 cccgttatca cggatataaa ccgtgtgatt gccgtccggc gcggtgacgc aactgacggc
    26881 gatttcgctg gcgttggcgt gcttcatcgc attcagcacc gcttcgcgaa taatttgcaa
    26941 caaatgcacc tgcatttgcg catccagcgc cagggttggc agacggcagt cgagggtcag
    27001 tttggcgctg gtttgatttt gtaacgtatc cagcatttcc ctcaacgcgg aggggagatc
    27061 cgcctgctgc agcgtcaggc ggaaagtagt caacagctcg cgtaactgcc gataagcatc
    27121 attcaacgcc tgggaaaagt cggccatgat actttgtgcg gtggcgttat cttccggtat
    27181 cgaacgcttc agtaacgtca actggatacg taagtaagaa agtacctgag ccagcgagtc
    27241 gtgcaattcg cgggcgatgg ttgcacgttc ttccatcaac aataactgct gaaaatgctt
    27301 ttgcgcctga ttaaagtaca aaccgcgtcc cagcatcgac gaaacgctgt taagcagcgg
    27361 ttctgatgat gaaacgtgac tattttgcca gtgcagttcg ccgtaaaccg tctcttgcat
    27421 tgtcaccggt aaaatctgca tcggcaattc cgggtttggt tgcccttcgc taatccgcca
    27481 gttttcaccg acatttaact ccagatattc agccgcttca ttgtcgcgaa caatctgcaa
    27541 aatatggcgg aaacaatgca catcaatctg gcttgtgttc agcgcctgcg aacattgata
    27601 caacacctcc agacgacgct tggcctcgtg gagatcgcgg gtcttttctt ctactgacgc
    27661 ctccagcgaa aggtacaatt tatgcagctc gctcgacatc tggttaaagg tttttgcaag
    27721 cagaccaagc tcattcggca gactggtatc cagcggcggc gagtcgaact gcccgtgttc
    27781 aatacgttga ctggcggtaa ccagctgatt cagcggggca accacctgat ggcgtatacg
    27841 gcgcaaagta aaaaagacca gcgtgaaaat accgataccg ccagccaggg aaatcgccac
    27901 caccagcagc attttgcgtt cagcgtagtg ctgtaaagcc agcacgaaca ggtctatctg
    27961 attaacataa ttattaatat tggcctgata ccacggcaaa tcgcccttgc tgagccgatt
    28021 attcatctcc agccagttgg cattcagatg cgcatagcga gtttttactg cttctggcac
    28081 ataccagacg ttgagattgg ttaataccga tgaatgcagt gcctgctgaa acaactggcg
    28141 atgtgcattg agttgtggac tgccactttg caagtcgtag cccaggcggt aactctgcat
    28201 acgcagcgat ccggcaatat tgatagcctc agcgtcgcgc aaactgctcg ccagagttag
    28261 cagagcgata cccgtggaaa gaatcgacag cagcacaatg taaaaaaagg cccgggccag
    28321 actggccgag acgggtcgtt taacaatcac acgcgtcttc tccacaaaaa atcattaatg
    28381 ttccagttcg ctaaaacgca atttagtcag tgatgggatt atttgatctg ccacagttta
    28441 tagcgaaata gttacttatt ctgggcacat gaacattgcc gattcccctg taagtcgggt
    28501 aataacaaca ctcatataaa gaataaggtt tttacaacca aaaaagaagg tcgttatgaa
    28561 tcgttttatt atggccaaca gtcagcaatg tctgggttgt catgcttgtg aaatcgcctg
    28621 tgtcatggct cacaatgatg agcaacatgt cctgagccaa caccattttc atccccgaat
    28681 tacggttatc aaacatcagc agcaacgtag tgcagtgacc tgtcaccatt gtgaagatgc
    28741 gccctgcgcc cgtagctgcc ctaatggcgc aatcagccac gttgatgaca gcattcaggt
    28801 caatcagcaa aagtgtattg gctgtaaatc ctgcgtggtg gcctgtccgt ttggtacgat
    28861 gcaaatcgtc ctgacacccg tcgcggcagg aaaagtaaaa gccacggcgc ataaatgcga
    28921 cctttgtgca gggcgcgaaa acggtcctgc ctgtgtcgag aattgcccgg cggacgcgct
    28981 gcaactggtc actgacgtcg cactctccgg catggcgaaa tcccgccgct tgcgcaccgc
    29041 acgtcaggag catcaaccgt ggcatgccag taccgctgca caagaaatgc cggtaatgag
    29101 taaagtcgaa caaatgcagg caacgcccgc gcgtggcgag ccggataaac tggcgattga
    29161 agcgcgcaaa accggttttg atgaaattta tctgccattc cgtgccgatc aggcacaacg
    29221 ggaagcctcg cgctgcctta aatgcggcga gcacagcgtt tgtgaatgga cctgcccgct
    29281 gcataaccat attccgcagt ggattgaact ggtgaaagcc ggaaacatcg acgccgccgt
    29341 cgagctttct cacctgacca acaccctgcc ggaaattacc ggacgcgttt gtccgcagga
    29401 ccgtttgtgt gaaggtgcct gtactattcg cgatgagcac ggcgcggtaa ctatcggcaa
    29461 cattgaacgc tacatttcag atcaggcgtt ggcgaaaggt tggcgtcctg acttaagcca
    29521 tgtcaccaaa gtggacaagc gggtggcgat tatcggtgcg ggtccggcag ggctggcctg
    29581 tgcggatgtt ctgacccgca atggcgtggc ggtgacggtg tacgatcgcc atccagaaat
    29641 tggtggcttg ctcactttcg gcattccttc tttcaaactg gataaatccc tgctggcacg
    29701 ccgtcgggaa atcttcagcg cgatggggat tcacttcgaa ctcaattgtg aagtgggtaa
    29761 agatgtctct ttggattcgc ttttggaaca atacgacgcg gtcttcgttg gcgtaggcac
    29821 ttaccgttcc atgaaagcgg gtttacccaa tgaagatgcg ccgggcgttt atgacgcgct
    29881 gccgttcctc atcgccaaca ctaaacaggt gatgggactc gaagagctac cggaagagcc
    29941 gtttatcaat accgccggac tcaacgtcgt ggtactgggc ggcggcgaca ccgcgatgga
    30001 ctgtgtgcgt accgcgctgc gccacggcgc gagtaacgtc acctgcgctt atcgtcgtga
    30061 tgaagctaac atgccaggct cgaagaaaga ggtgaagaac gcccgcgaag agggggccaa
    30121 cttcgaattt aacgtccagc cggtggcgct tgagctgaat gagcaaggtc acgtttgcgg
    30181 aattcgtttc ctgcgcacgc gtcttggcga gccggatgcc caggggcgcc ggcgacttgt
    30241 accgattgag ggcagtgaat ttgtcatgcc agccgatgcg gtgattatgg tgtttggctt
    30301 caatccacac gggatgccgt ggctggagtc gcacggtgta acggtagaca aatggggccg
    30361 catcatcgcg gatgtggaaa gccagtaccg ttaccagacc accaatccga aaatcttcgc
    30421 cggtggcgac gccgtgcgtg gtgcggatct ggtggttacc gcaatggcag aaggacgtca
    30481 tgcggcacag gggattattg actggctggg ggtaaaatca gtcaaatctc actgatagcc
    30541 tgcgcagaca aacccgactt cacagcgtaa tataattgtt catttcgcgc tgtggagtcg
    30601 gtatgacgca acaaatcacc ctcattaaag acaaaattct ctccgataac tatttcaccc
    30661 tgcacaacat tacttacgat ctcacgcgca aagatggcga agttatccgc cataaacgtg
    30721 aagtttacga tcgcggtaat ggtgcgacga tcctcctgta caacgcgaag aaaaagaccg
    30781 tggttctgat tcgtcagttc cgtgtcgcta cctgggttaa tggcaatgaa agcgggcagc
    30841 tgattgaaac ctgcgccggg ctgctggata acgacgaacc ggaagtgtgc attcgtaaag
    30901 aagcgattga agagacgggc tatgaagtgg gcgaagtgcg caaattattt gaactgtata
    30961 tgtcgcctgg cggtgtgact gagctaatcc acttttttat cgccgaatac agtgacaatc
    31021 agcgcgctaa cgccggtggc ggtgttgaag atgaagatat tgaagtgctt gagctgccgt
    31081 tcagccaggc gctggagatg atcaaaaccg gcgagatacg tgacggtaag acggtgttat
    31141 tgcttaacta tttgcaaacg tcacatttaa tggactgaaa aataacaata atatttcgtt
    31201 gtttattatt ggctcaatcc gacaaatctg attgagccgc gctacctgcg caacgaagat
    31261 acgacttgtg ctgtttgttt gaacttctgg ggtcgtaccg tccatgcgct atcgcatttt
    31321 ccttctcttt tttttcgctt tgttgccgac gtctttggtg tgggcggcac tagcgcaacg
    31381 ggcgttttcc gactggcagg tcacctgtaa taaccaaaac ttctgcgtgg cgcgcaatac
    31441 gggcgatcat aatggactgg tgatgaccct gagccgcagc gccggggcgc ataccgatgc
    31501 cgttttacgt attgagcgcg gcgggttgaa gtcgccggat gcgtctgaag gagagatagc
    31561 gccacggctg ctgttagatg gtgagccgtt ggcgttaagt ggtgacaagt ggcggatttc
    31621 accatggtta ttagtaaccg atgatacggc aaccatcacc gcgtttttgc agatgattca
    31681 ggaagggaag gcgatcacct tacgcgacgg caatcagacc atttctctga gtggcttaaa
    31741 agcggcgttg ttgtttattg atgctcagca aaagcgcgtt ggcagtgaaa ccgcgtggat
    31801 caagaaaggg gacgaaccgc cgctcagcgt accgcccgcg cctgcgctga aagaggtcgc
    31861 ggtggttaac ccaacgccga cgccactctc actcgaagaa cgcaacgatt tgctggatta
    31921 tggaaactgg cggatgaatg gtctgcgctg ctcgcttgat ccgttacgtc gtgaggtgaa
    31981 tgtcactgcg ctgactgatg ataaagcgct gatgatgatc agctgtgagg caggggccta
    32041 taacaccatt gatttggcat ggattgtgtc gcgtaaaaag ccgctagcct cgcgcccggt
    32101 tcggttgcgt ttgccgttca acaacgggca ggagacgaat gaactggaac tgatgaacgc
    32161 aacatttgat gagaaatcgc gtgaactggt gaccttagcg aaagggcgcg gattaagcga
    32221 ttgtggcatt caggcgcgct ggcgctttga cggtcagcgt tttcgcctgg tgcgttacgc
    32281 cgcagaaccc acctgcgata actggcatgg gccagacgcc tggcccacgt tgtggatcac
    32341 ccgttaaaca aaatggcggg gacatgtagg ccggataaga cgctgtaggc cggataagac
    32401 gcgtcagcgt cgcatccggc aatcagcatc cggcgatcac tatcaggcac ctttcactcc
    32461 cagcacctta tgcgcttttg ccacaatatt ctcggcggta aagccaaaga acgggaacag
    32521 cttatccgcc ggagcagatt ccccataacc cgtcatcccg acaattgccc ctttcagacc
    32581 aacatacttg taccagtaat cggcaatacc tgcttccacc gccacgcgag ccgcaacgtt
    32641 agaaggcaac accgactccc gatattcctc atcctgggcg tcgaaaatat cggtcgaggg
    32701 cagggaaacc acgcgaacat tgcgaccttc tcctgctaat ttctccgcag cttgcagggt
    32761 gatttccatc tctgaaccgg tggcaatcag aataatatct ggcttaccgc cgctgtcttt
    32821 cagcacataa ccgccacgag taatctcttt aacctgatcc ggcgtacgtt ccacctgggc
    32881 cagattttgc cttgagagga tcagtgccgt cggtccgttg tggcgctcaa ccgccagctt
    32941 ccagcccacc gccgcttcca cctgatcgca cggtcgccag gtgctgaaat ttggcgttaa
    33001 gcgcaggctg gccagttgct caacagcctg gtgcgtcgga ccatcttcgc ccaggccaat
    33061 tgagtcgtgg gtataaacca taatctgccg cgctttcatc agtgccgcca tccgcgcggc
    33121 gttacgggcg tattcaacaa acatcaggaa cgtcgcggta tacggcacaa agccgccgtg
    33181 gtgcgcgatg ccgttggcga tagcggtcat gccaaattca cgcaccccgt agtgaatgta
    33241 gttgcccgcc ggatcttcct tcagcgaaac agaacctttc cagatggtca ggttgctggg
    33301 agccagatcc gccgaaccgc cgagcagctc cggtagcaag gggccataag cgttaagcgt
    33361 attttgcgaa gccttacggg tggcgatttt cgccggattc gcctgcaact cattgatata
    33421 cttctgagtc gttttctccc agtccttcgg taaaccaccg ctcatccgac gggtaaactc
    33481 ttctgccagt tgcggatgag cctttttata ggcggcaaac ttctcattcc acctctgctg
    33541 cgctttttcg cctttttcgc gggcatccca ggcgtgataa atatctttag ggatctcaaa
    33601 tggcggatgg tgccagccca gtttttgccg tgccagcgcc acttcttctt cccccagcgg
    33661 tgcgccgtgc gcctcttcct tacctgcttt attcggcgat ccaaagccaa tcaccgtacg
    33721 acagataatc agcgacggct tatctttcac gctttgcgct tcaaggatcg cttccttcac
    33781 cgcctgcgga tcgtgaccgt cgatttcatg gatcacatgc cagtgatagg cttcaaaacg
    33841 ttttgccgta tcgtcggtaa accagccttc tgtttcaccg tcgatggaaa taccgttgtg
    33901 atcgtaaaaa ccaatcagct tgcccagtcc cagcgtgcct gccagcgaac agacttcgtg
    33961 ggaaatacct tccatcaggc agccgtcgcc cataaacaca taggtgaagt gatcgacgat
    34021 ctcatggtct ggctggttaa actgcgccgc cagtgtacgc tctgctatcg ccagcccgac
    34081 ggcgttcgcc aaaccttgtc caagtggacc ggtggtggtt tcaacgcctg gcgtataacc
    34141 aatctccggg tggcctgggg ttttcgaatg cagttgacga aagttcttca gctcttccag
    34201 cggcaggtcg taaccggtca gatgcagcaa actgtagagc agcatcgacg cgtgaccgtt
    34261 ggaaagaata aagcggtcgc gatcatacca ggttgggtcg gtagggttat gtttaagaaa
    34321 atcgttccac agcacttcgg caatatcagc catgcccatc ggcgcgccgg gatgaccaga
    34381 attggctttt tgtaccgcat ccatactgag tgcgcgaatc gcattggcaa ggtcttttcg
    34441 ggacatataa cactccgtgg ctggtttata gtttggcggc aagaagatct tccagtttgc
    34501 gttgatcaac ggcgaacaga cgaatgcctt cagacagttt ttctaccgcc atcgcatact
    34561 gattgtgctc ccaacggaac tccgcttcgc tcatgggagc tgggcgtggg aacgtctgag
    34621 aaggtgggat taatttacgt accactggcg aaactttttc ctgcagctcc ttcagcaaat
    34681 tcggtgcgat agtcagtcga tcgcagccgg ttaaggcgag gatttgttcg gtgcgacgga
    34741 agctcgcgcc catcacaatg gtttcatagt ggtgttgctt atagtagtcg tagatattgc
    34801 gcaccgattt aacgcccgga tcttcttcca ccacatacgg gtccatcggc ttgcgtgcct
    34861 gataccagtc ataaatacgc ccgacaaacg gcgaaatcag aaaaacgcct gcttccgcac
    34921 aggcccgtgc ctgtgcaaaa gaaaacagca gcgtcaggtt gcagttaata ccttcttttt
    34981 ccagctcttc tgccgcgcga attccttccc aggtcgaagc cagcttgatc agaatgcgtg
    35041 atttctcaac gccttgttgc tgatacaagt ccaccagatg gcgcgccttc tcaatactct
    35101 tttctttatc aaaagagagg cgtgcatcaa cttctgttga cacgcgaccg ggtacgattt
    35161 tgaggatttc agcaccgaaa ttgaccgcca gtttgtcaca cgctgcgacc acctgttgtt
    35221 cctgggtctt gccatttttt ttaccccagg cgatagcatc gtctattaaa tgctcatatt
    35281 gtgataatcc ggcagccttg agtaacagcg aaggattggt ggtggcatcc tggggatgat
    35341 aatggcgaat ggactcaata tcgccgctgt ctgccacgac agtggtgaac tgtttgatgc
    35401 cgtctaactc gttcatagga aatactcctt gaaaagtaaa gtgttagatg agtgcgttaa
    35461 ttcacacttc tgagaaattt cgctaaacgc atcaaaaaag catagcagac aggcatggta
    35521 ttgctggatt aagcaggtaa catcagtgtt ataggattat taccaaaaca ttatattaat
    35581 ggtgagagtt tggacttgct caaagtctgt agactccggc aaggtaataa tgtgcgccac
    35641 gttgtgggca gggggtgttg cccacacact ttatttgtga acgttacgtg aaaggaacaa
    35701 ccaaatggat gaccagttaa aacaaagtgc acttgatttc catgaatttc cagttccagg
    35761 gaaaatccag gtttctccaa ccaagcctct ggcaacgcag cgcgatctgg cgctggccta
    35821 ctcacccggc gttgccgcac cttgtcttga aatcgaaaaa gacccgttaa aagcctacaa
    35881 atataccgcc cgcggtaacc tggtggcggt gatctctaac ggtacggcgg tgctggggtt
    35941 aggcaacatt ggcgcgctgg ctggcaaacc ggtgatggaa ggcaagggcg ttctgtttaa
    36001 gaaattcgcc gggattgatg tatttgacat tgaagttgat gaactcgacc cggacaaatt
    36061 tattgaagtt gtcgctgcgc tcgaaccgac cttcggcgga atcaacctcg aagacattaa
    36121 agcgccagaa tgtttctata ttgaacagaa actgcgcgag cggatgaata ttccggtatt
    36181 ccacgacgat cagcacggca cggcaatcat cagcactgcc gccatcctca acggcttgcg
    36241 cgtggtggag aaaaacatct ccgacgtgcg gatggtggtt tccggcgcgg gtgccgcagc
    36301 aatcgcctgt atgaatctgc tggtagcgct gggtctgcaa aaacataaca tcgtggtttg
    36361 cgattcaaaa ggcgttatct atcaaggccg tgagccaaac atggcggaaa ccaaagccgc
    36421 atatgcggtg gtggatgacg gcaaacgtac cctcgatgat gtgattgaag gtgcggatat
    36481 tttcctgggc tgttccggcc cgaaagtgct gacccaggaa atggtgaaga aaatggctcg
    36541 ggcaccaatg attctggcgc tggcgaatcc ggaaccggaa attctgccgc cgctggcgaa
    36601 agaagtgcgt ccggatgcca tcatctgcac cggtcgttct gactatccaa accaggtcaa
    36661 caacgtcctt tgcttcccgt tcatcttccg tggcgcgctg gacgttggcg caaccgccat
    36721 caacgaagag atgaaactgg cggcggtacg cgcgattgca gaactggccc atgcggaaca
    36781 gagcgaagtg gtggcttcag cgtatggcga tcaggatctg agctttggtc cggaatacat
    36841 cattccaaaa ccgtttgatc cgcgcttgat cgttaagatc gctcctgcgg tcgcgaaagc
    36901 ggcgatggag tcgggcgtgg cgacgcgtcc gattgctgat ttcgacgtct acatcgacaa
    36961 gctgactgag ttcgtttaca aaaccaacct gtttatgaag ccgattttct cccaggctcg
    37021 caaagcgccg aagcgcgtag ttctgccgga aggggaagag gcgcgcgttc tgcatgccac
    37081 tcaggaactg gtaacgctgg gactggcgaa accgatcctt atcggtcgtc cgaacgtgat
    37141 cgaaatgcgc attcagaaac tgggcttgca gatcaaagcg ggcgttgatt ttgagatcgt
    37201 caataacgaa tccgatccgc gctttaaaga gtactggacc gaatacttcc agatcatgaa
    37261 gcgtcgcggc gtcactcagg aacaggcgca gcgggcgctg atcagtaacc cgacagtgat
    37321 cggcgcgatc atggttcagc gtggcgaagc cgatgcaatg atttgcggta cggtgggtga
    37381 ttatcatgaa cattttagcg tggtgaaaaa tgtctttggt tatcgcgatg gcgttcacac
    37441 cgcaggtgca atgaacgcgc tgctgctgcc gagtggtaac acctttattg ccgataccta
    37501 cgtcaatgac gaaccagacg cagaagagct ggcggagatc accttgatgg cggcagaaac
    37561 tgtccgtcgt ttcggtattg aaccgcgcgt tgctctgttg tcgcactcca actttggttc
    37621 ttctgactgc ccgtcgtcga gcaaaatgcg tcaggcgctg gaactggtca gggaacgtgc
    37681 gccagaactg atgattgatg gtgaaatgca cggcgatgcg gcgctggtgg aagcgattcg
    37741 caacgaccgt atgccggaca gccctttgaa aggttctgcc aatattctgg tgatgccgaa
    37801 catggaagct gcccgcatta gttacaactt actgcgcgtt tccagctcgg aaggtgtgac
    37861 tgtcggtccg gtgctgatgg gcgtggcgaa accggttcac gtgttaacgc cgatcgcatc
    37921 ggtgcgtcgt atcgtcaaca tggtggcgct ggccgtggtt gaagcgcaaa cccaaccgct
    37981 gtaattcttt tttaactctc acgcttaccc tgaatattca gggtaagcgg tttggctgca
    38041 atatattagt aaagcttatt actgagtttg cgaataataa aaaaagcagt ctatataata
    38101 tctcgatatt attttattta tattcatgcg ttgcatatga aagtttatgc accacagcga
    38161 atatctctcc attcttagtg atctacctca ccttttaaac gcgcttgccg aattttgtta
    38221 tttactctga cgaaaaattg tcacgataca cgaaagtttt tcacaggcgg cgactcatgg
    38281 ataaagaacg catcattcag gaatttgtgc ccggcaaaca ggtcacactg gcgcatctca
    38341 tcgcgcatcc cggtgaggaa ctggcgaaga aaatcggcgt tccggatgcg ggcgtaattg
    38401 gcattatgac gctaactccc ggcgaaacgg cgatgatcgt cggtgattta gcgctgaaag
    38461 ccgctgatgt gcatatcggt tttctcgacc gcttcagcgg tgcattggtg atttatggct
    38521 cggtgggcgc agtagaagaa gcgttatcac aaaccgtcag cggtttaggt cgcttattaa
    38581 attatacgct ttgtgaaatg accaaaagtt aatacgaggc attcatgaaa cgaattgcgt
    38641 ttgtgggtag cgtcggggca ggaaaaacaa cgttatttaa tgcgttgcag gggaattata
    38701 ccctcgccag aaaaacacag gccgtggaat ttaatgataa gggcgatatt gatacgccgg
    38761 gtgaatattt tagccatccc cgctggtatc acgccttaat taccacgcta caggatgtag
    38821 acatgctgat ctatgtccat ggcgcaaatg acccggaaag tcggttgcct gccgggttgc
    38881 tggatattgg cgtcagcaaa cggcaaatcg ccgtcatcag taaaacggac atgccagatg
    38941 ctgacgtcgc cgcaacgcga aagttattgc ttgagacggg tttcgaagag ccgattttcg
    39001 agctaaacag ccacgacccg caaagcgtgc agcaactggt ggattatctg gcatctctca
    39061 ccaaacagga ggaagcaggt gaaaaaactc atcacagcga atgatattcg tgaagcacac
    39121 gcgcgcggcg aacaggcaat gtccgtggtt ctgcgcgcca gcattattac cccggaagcc
    39181 cgtaaagtgg cggatctact gggttttacc atcaccgaat gtgatgaatc gatcccggta
    39241 acggcgtccg tgcccgccag cgtgccagcc gacaaaaccg aaagccagcg catccgcgaa
    39301 accatcattg cccagctgcc ggaaggccag tttaccgaaa gcctggtcgc acagctgatg
    39361 gaaaaagtga tgaaggaaaa acagtcgctg gagcagggcg caatgcagcc gagcttcaaa
    39421 tcggtgaccg gcaaaggcgg catcaaagtg attgatggca gcagcgtcaa gtttggtcgt
    39481 tttgatggcg cacaaccgca ttgcgtgggc ttgactgatc ttgtgaccgg agacgacggc
    39541 agcagcatgg ccgccggttt tatgcagtgg gagaacgcat tcttcccgtg gaccctgaac
    39601 tacgacgaaa tcgacatggt gctggaaggg gagctccatg ttcgccacga aggcgaaacc
    39661 atgatcgcca aagcaggcga cgtgatgttt atcccgaaag gctccagcat cgaatttggt
    39721 acgacatcca gcgtgaaatt cctgtatgtc gcctggccgg ctaactggca atccctatga
    39781 aagatttcat caccgaagca tggctaagag cgaaccatac gctcagcgaa ggcgcagaga
    39841 tccatctccc tgcggacagt cgcctgacgc cctctgcccg ggagttgctg gaaagccgcc
    39901 atctgcgcat caagtttatt gacgagcagg gccgcctgtt tgttgacgat gaacagcagc
    39961 agccgcagcc cgttcatggg ctgaccagta gcgatgaaca tccgcaggcg tgctgcgaac
    40021 tgtgccgcca gccggtggca aaaaagccgg atacgctgac tcacctgtcg gcggaaaaaa
    40081 tggtcgccaa aagcgatccg cgtctgggtt ttcgcgcggt tctcgacagc accattgcgc
    40141 tggcagtgtg gctgcaaatt gaactggcgg aaccgtggca gccgtggctg gcggatatcc
    40201 gttcgcgtct tggcaacatt atgcgcgccg atgcgctggg ggaaccgctg ggctgccagg
    40261 cgattgtcgg gctttctgac gaagatctgc atcgactttc tcaccagccg ctgcgctatc
    40321 tcgaccacga tcatctggtg ccggaagcca gccacgggcg cgatgcggcg ttgctcaatc
    40381 tgctacgtac caaagtgcgg gaaaccgaaa ccgtagcggc gcaggtgttt atcacccgca
    40441 gctttgaagt attacgcccg gacatcttgc aggcgctgaa ccgcctttcg agcacggtct
    40501 acgtaatgat gatcctgagc gtaacgaagc cgccgctaac ggtgaagcag atccaacaac
    40561 gactggggga aacgcaatga ttattgaacg ttgtcgtgaa ctggcgttgc gagcgcccgc
    40621 cagagtggtt tttccggatg cgttagatca acgtgtgctg aaagctgcgc aatatttaca
    40681 tcaacaaggt ctggcaacgc ccattctggt cgctaatccg tttgaacttc gtcagtttgc
    40741 gctcagtcac ggcgtggcga tggacgggct acaggtgatt gatccgcatg gcaacctcgc
    40801 aatgcgggaa gaatttgctc atcgctggct ggcccgcgcg ggcgaaaaaa cgccgccgga
    40861 tgcgctggaa aaacttaccg atccgctgat gttcgccgca gcaatggtca gcgccggtaa
    40921 agcggatgtc tgtatcgcgg gcaacctctc ttccacggcg aatgtgctgc gtgccggatt
    40981 acgtattatc ggcttgcagc caggctgtaa aacgctctca tccattttcc tgatgctgcc
    41041 acagtacagc ggtccggcgt tgggctttgc cgattgcagc gtggtgccac agccgacggc
    41101 ggcgcaactg gcggatatcg cgcttgccag tgccgaaacc tggcgcgccc tcaccggaga
    41161 agagccgcgc gtggcgatgc tgtcgttttc cagtaacggt agcgcccgtc acccctgcgt
    41221 tgccaacgtc cagcaggcga cagaaatcgt ccgtgagcgc gcaccaaagc tggtggttga
    41281 tggcgagttg cagtttgacg ccgccttcgt gccggaagtg gcggcgcaaa aagcgcctgc
    41341 cagcccgcta cagggcaaag ccaatgtgat ggtttttccg tcgctggaag ccggaaatat
    41401 tggctacaaa atcgcacaac gactcggcgg atatcgtgcc gtcgggccat tgatacaagg
    41461 acttgccgcg ccgatgcacg atctctctcg tggttgtagc gtgcaggaaa ttatcgagct
    41521 ggcgctggtg gcagctgtgc cgcgtcagac agaagtgaac cgcgaaagca gtttacaaac
    41581 actggttgaa tgaacggtcc cgttctggac cctgataaag aggaaagcac aatggaagca
    41641 ttaggaatga tcgaaacccg gggcctggtt gcactgattg aggcttctga cgcgatggtt
    41701 aaagctgcgc gcgtcaagct ggttggtgtt aagcagattg gcggcggcct gtgtactgcc
    41761 atggttcgtg gtgatgttgc agcctgtaaa gccgcgaccg atgcaggtgc cgcagcggca
    41821 cagcgtatcg gcgagctggt atctgtacac gttatcccgc gtccgcacgg cgacctggaa
    41881 gaagtcttcc cgatcggcct gaaaggcgat agcagcaacc tgtaatcacc ctgtgtgacg
    41941 tcgattcggg cgtcacgctc tctccttttt cattttacct tctgcggagg gtagggggag
    42001 cttttacctg aaatacgcca cggaggcggg tatgaaactg gcagtcgtca ctggacaaat
    42061 tgtttgtacc gtacgccatc acggactggc gcatgacaaa ttgctgatgg tggaaatgat
    42121 tgatccacaa ggtaatcctg acgggcaatg cgccgtcgcc atcgacaata ttggcgcggg
    42181 aaccggggag tgggtgttac tggtgagtgg cagttccgcc cgccaggcgc ataaaagcga
    42241 aacgtcaccg gtcgatctgt gcgtgattgg cattgtcgat gaggtggtgt ctggcggtca
    42301 ggtaattttc cacaaataag gcagaacatc atgaatcaac aggatattga acaggtggtg
    42361 aaagcggtac tgctgaaaat gcaaagcagt gacacgccgc ccgccgccgt tcatgagatg
    42421 ggcgttttcg cgtccctgga tgacgccgtt gcggcagcca aagtcgccca gcaagggtta
    42481 aaaagcgtgg caatgcgcca gttagccatt gctgccattc gtgaggcagg cgaaaaacac
    42541 gccagagatt tagcggaact tgccgtcagt gaaaccggca tgggccgcgt tgaagataaa
    42601 tttgccaaaa acgtcgctca ggcgcgcggc acgccaggcg ttgagtgtct ctctccgcag
    42661 gtgctgaccg gcgacaacgg cctgacttta attgaaaacg cgccctgggg cgtggtggct
    42721 tcggtaactc cgtccactaa cccggcggca accgtaatta ataacgccat cagcctgatt
    42781 gccgcgggca acagcgtcat ttttgccccg catccggcgg cgaaaaaagt ctcccagcgg
    42841 gcgatcacgc tgctcaacca ggcgattgtt gccgcaggcg ggccggaaaa cttactggtt
    42901 actgtggcaa atccggatat cgaaaccgcg cagcgtttgt tcaagtttcc gggcatcggc
    42961 cttctggtgg tgaccggcgg cgaagcggta gtagaagcgg cgcgtaaaca caccaataaa
    43021 cgtctgattg ccgcaggcgc tggcaacccg ccggtagtgg tggatgaaac cgccgacctc
    43081 gcccgtgccg ctcagtccat cgtcaaaggc gcttctttcg ataacaacat catttgtgcc
    43141 gacgaaaagg tactgattgt tgttgatagc gtagccgatg aactgatgcg tctgatggaa
    43201 ggccagcacg cggtgaaact gaccgcagaa caggcgcagc agctgcaacc ggtgttgctg
    43261 aaaaatatcg acgagcgcgg aaaaggcacc gtcagccgtg actgggttgg tcgcgatgcg
    43321 gcgaaaatcg cggcggcaat cggcctgaac gtgccgcaag aaacgcgttt gctgtttgtg
    43381 gaaaccactg cagaacatcc gtttgccgtg accgaactga tgatgccggt gctgcccgtc
    43441 gtgcgcgtcg ccaacgtggc ggatgccatt gcgctggcgg tgaaactgga aggcggttgc
    43501 caccacacgg cggcaatgca ctcgcgcaac atcgaaaaca tgaaccagat ggcgaatgct
    43561 attgatacca gcattttcgt taagaacgga ccgcgcattg ccgggctggg gctgggcggg
    43621 gaaggctgga ccaccatgac catcaccacg ccaaccggtg aaggggtaac tagcgtgcgt
    43681 acgtttgtcc gtctgcgtcg ctgtgtatta gtcgatgcgt ttcgcattgt ttaaggagta
    43741 acagatggcg cacgacgaac aatggctcac cccacgtctg caaacggcgg caacgctgtg
    43801 taaccagacg cccgccgcga cggaatctcc gctgtggctg ggggtggatc tgggcacctg
    43861 cgatgtggtg tcgatggttg tcgaccgcga cggtcagtcg gtggcggtat gcctcgactg
    43921 ggccgatgtg gtccgcgacg gcatcgtctg ggatttcttc ggcgctgtca ccattgttcg
    43981 tcgccatctc gacacgctcg agcagcaatt cggtcgccgt tttagccatg cggcgacttc
    44041 atttcctcca ggtacggacc cgcgtatttc catcaacgtg ctggagtctg ctggactgga
    44101 agtgagccat gtgctggatg agccaaccgc agttgcggat ctgctgcaac tggacaacgc
    44161 cggtgtagtg gatatcggcg gcggcactac cggcattgcc atcgtgaaaa agggcaaggt
    44221 gacgtactcg gcggatgaag caaccggcgg acatcacatc tctctgactc tcgccggaaa
    44281 tcgccgtatt tcgctggaag aggcggagca gtacaagcgc ggtcacggtg aagagatttg
    44341 gcctacggtg aaaccggtgt acgaaaaaat ggtggacatt gtcgcccggc atattgaagg
    44401 gcagggcatt actgatttat ggctggcggg cggctcttgt atgcaaccgg gcgtggcgga
    44461 actgtttcgc aaacaattcc cggcgttaca ggtgcattta ccgcagcaca gtttgtttat
    44521 gaccccgctg gcgatcgcca gtagtgggcg agagaaagcg gaggggctat atgcaaaatg
    44581 aattgcagac cgcgctcttt caggcgttcg ataccctgaa tctgcaacgg gtaaaaacat
    44641 ttagcgttcc accggtgacg ctttgcggtc cgggctcggt gagcagttgc ggacagcaag
    44701 cgcaaacgcg tgggctgaaa catctgttcg tgatggcaga cagctttttg catcaggcag
    44761 ggatgaccgc cgggctgacg cgcagcctgg ctgttaaagg catcgccatg acgctctggc
    44821 catgtccggt gggcgaaccg tgcatcaccg acgtgtgtgc agccgtggcg cagttgcgtg
    44881 agtcaggctg tgatggggtg atcgcatttg gcggcggctc ggtgctggat gcggcgaaag
    44941 ccgtggcgtt gctggtgacg aacccggata gcacgctggc agagatgtca gaaaccagcg
    45001 ttctgcaacc gcgcttgccg ctgattgcca ttccaacgac cgccggaacc ggctctgaaa
    45061 ccaccaatgt aacggtgatt atcgacgcgg tgagcgggcg caagcaggtg ttagcccatg
    45121 cctcgctgat gccggatgtg gcgattctcg acgccgcgtt gaccgaaggt gtgccgtcgc
    45181 atgtcacggc gatgaccggc attgatgcgt taacccatgc cattgaagca tacagcgccc
    45241 tgaacgctac accgtttacc gacagtctgg cgattggtgc cattgcgatg attggcaaat
    45301 cgctgccgaa agcggtgggc tacggtcacg accttgccgc gcgcgagagc atgttgctgg
    45361 cttcatgtat ggcgggaatg gcgttttcca gtgcgggtct tgggttgtgc cacgcgatgg
    45421 cgcatcagcc gggcgcggcg ctgcatattc cacacggtct ggcgaacgcc atgttgctgc
    45481 caacggtgat ggaatttaac cggatggttt gtcgtgaacg ctttagtcag attggtcggg
    45541 cactgcgaac taaaaaatcc gacgatcgtg acgctattaa cgcggtaagt gagctgattg
    45601 cggaagttgg gattggtaaa cgactgggcg atgttggtgc gacatctgcg cattacggcg
    45661 catgggcgca ggccgcgctg gaagatattt gtctgcgcag taacccgcgt accgccagcc
    45721 tggaacagat tgtcggactg tacgcggcgg cgcaataaat gccggatgcg acgcttgccg
    45781 cgtcttatcc agcctacggg attgcacatg tagggcggat aaggcgttca cgccgcatcc
    45841 gccagtggcg cggtgcaatt gccggatgcg atgcttgccg cgtcttatcc ggcctacggg
    45901 attgcacatg tagggcggat aaggcgttca cgccgcatcc gccagtggcg cggtgcaatt
    45961 gccggatgcg acgcttgccg cgtctgatcc ggccttcggg attgcacatg tagggcggat
    46021 aaggcgttta cgccgcatcc gccaataaat aacaactaac agggagtaaa ggcgatggga
    46081 attaacgaaa tcatcatgta catcatgatg ttctttatgc tgatagctgc cgtagacagg
    46141 atcctgtcgc agttcggcgg ttctgctcgt ttcctcggta agttcggtaa aagtatcgaa
    46201 ggatcaggcg gtcagttcga agaaggcttt atggcaatgg gcgctctggg tctggcgatg
    46261 gtcggtatga ccgcgctggc accggtactg gctcacgtac tcgggccggt aattatcccg
    46321 gtttacgaaa tgctcggcgc aaacccgtca atgttcgccg gaacgctgct ggcgtgcgat
    46381 atgggcggct tcttcctcgc caaagagctg gcgggcggcg acgtagcagc gtggctatac
    46441 tctgggttaa ttcttgggtc gatgatgggg ccaacgattg tgttttccat tccggtggcg
    46501 ctcggcatta tcgaaccttc tgaccgtcgt tatctggcgc tcggcgtgct ggcgggcatt
    46561 gtgaccattc cgattggctg tattgctggt ggtctggttg ctatgtactc cggtgtgcag
    46621 atcaacggtc agccggtgga attcactttc gccctgatcc tgatgaacat gatcccggtg
    46681 atcattgttg cgattctggt ggcgctgggg ctgaaattca tcccggaaaa aatgatcaac
    46741 ggcttccaga tcttcgccaa attcctcgtt gcattgatca ccctcggtct tgctgccgcg
    46801 gtagttaagt ttctgcttgg ctgggaactg atccccggtc tggaccctat ctttatggcc
    46861 cctggcgata aacccggtga agtgatgcgc gccattgaag ttatcggttc tatctcctgc
    46921 gttctgttag gggcgtatcc aatggtgctg ctgctgaccc gctggtttga aaaaccgctg
    46981 atgagcgtcg gtaaagtgct gaatatgaac aacatcgcgg cagccggcat ggtggcaacg
    47041 cttgccaaca acatcccgat gttcggcatg atgaagcaga tggatacccg cggcaaagtc
    47101 atcaactgcg ccttcgccgt ttccgctgct ttcgccctgg gcgaccactt aggcttcgcc
    47161 gctgccaaca tgaacgccat gatcttcccg atgattgtcg gcaagttgat cggcggcgta
    47221 acggcgattg gcgtggcgat gatgctggtg ccaaaagaag acgcgaccac ggctaaaacc
    47281 gaagcggagg cacaatcgtg aacactcgcc agctattgag cgtcggtatc gatatcggca
    47341 ccaccaccac ccaggtgatt ttctcccacc tggagctggt taaccgtgcg gcggtgtcgc
    47401 aggtgccgcg ctacgaattc attaaacgcg aaattagctg gcaaagcccg gtgttcttta
    47461 cccctgtcga taaacagggt ggtttaaaag aagcggaact gaaaacctta atcctcgagc
    47521 aatatcaggc tgcgggcatt gcgccggaaa gcgttgattc tggtgccatc atcatcaccg
    47581 gtgaaagcgc gaaaacccgc aatgctcgtc cggcggtgat ggtgctctcc cagtcgctgg
    47641 gcgattttgt cgttgccagc gccgggccgc acctcgaatc cgtcattgcc ggtcacggtg
    47701 ccggggcaca aaccctttct gaacaacggc tgtgtcgggt actgaatatt gacatcggcg
    47761 gtggcactgc gaactacgcc ctgttcgatg ccggaaaaat cagcggcact gcctgcctca
    47821 acgtcggtgg tcgcctgctg gaaaccgaca gccaggggcg cgtggtttac gctcataaac
    47881 cggggcagat gattgtggat gagtgcttcg gcgcaggcac cgacgcccgt tcgctgaccg
    47941 gcgcgcagct ggtgcaggtt acccggcgga tggcagaact gattgtcgaa gtgattgacg
    48001 gaacgctctc gccgctcgcg caggcattga tgcaaaccgg tttgctgccc gcaggtgtta
    48061 cgcccgaaat cattacgctt tctggcggcg tgggcgaatg ttatcgccac cagcccgccg
    48121 acccgttctg ttttgccgat attggcccgc tgctggcaac ggcgctgcat gaccatccgc
    48181 gcctgcgtga gatgaacgta cagtttccgg cgcaaaccgt gcgcgccacg gtgattggcg
    48241 cgggcgcgca taccctttcg ctctctggca gcacaatctg gcttgagggc gtacaactgc
    48301 cgctgcgcaa tttgccggtg gcgatcccga ttgatgaaac ggatctggtg agtgcctggc
    48361 aacaggcgct gcttcagctg gatcttgatc ccaaaactga cgcgtacgtg ctggcgcttc
    48421 ccgcctcgct gcctgtgcgt tacgccgcgg tactgacggt catcaacgcg ctggtcgatt
    48481 tcgtcgcgcg ttttccaaat ccgcatcccc tgctggtggt ggccgggcag gactttggta
    48541 aagctctggg catgttgttg cgcccacagc tacaacaact cccgttggca gtcattgacg
    48601 aagtgattgt ccgcgcgggg gactatatcg acattggtac gcctcttttt ggcggatcgg
    48661 ttgtgccggt gacggtgaaa tcactcgcat ttccttcctg agggaacgac ttatgaaact
    48721 aaagaccaca ttgttcggca atgtatatca gtttaaggat gtaaaagagg tgctggctaa
    48781 agccaacgaa ctgcgttcgg gggatgtgct ggcgggcgtt gcagcggcaa gctcacagga
    48841 gcgcgtggcg gcaaagcagg tgttgtcgga aatgaccgta gcggacatcc gcaataatcc
    48901 ggtgattgcc tatgaagatg actgcgtgac gcggctgatt caggacgatg ttaacgaaac
    48961 ggcctacaac cagattaaaa acaggagcat cagcgaactg cgtgagtatg tgctgagcga
    49021 tgaaaccagc gtggacgaca ttgcctttac ccgcaaaggg ctgacctcgg aagtggtcgc
    49081 ggcggtagcg aagatttgct ccaacgcgga cctgatctac ggcgcgaaga aaatgccggt
    49141 aatcaaaaag gccaatacca ccatcggtat tccgggcacc ttcagcgccc gtttgcagcc
    49201 aaatgacacc cgtgacgacg tgcaaagtat cgctgcacaa atctacgaag ggctttcctt
    49261 cggggtgggc gatgcggtga tcggcgttaa cccggtaact gacgacgtgg aaaacttaag
    49321 ccgcgtgttg gacaccattt acggcgtgat cgacaaattc aacatcccaa ctcagggctg
    49381 cgtactggcg cacgtcacca cccagatcga agcgattcgt cgcggcgcgc ctggcggact
    49441 gattttccag agtatctgtg gcagcgaaaa agggctgaaa gagtttggcg tggaactggc
    49501 gatgctcgac gaagcgcgcg cagtgggtgc tgagttcaac cgtatcgccg gggaaaactg
    49561 cctctacttc gaaaccgggc aaggttcggc gctgtctgct ggcgctaact tcggcgcaga
    49621 ctaggtgacg atggaagcac gtaactatgg gctggcgcgt cattacgatc cgtttatcgt
    49681 caacaccgtg gtcggcttta ttgggccgga gtatctctac aacgaccgcc agattatccg
    49741 tgctggctta gaagatcact ttatgggcaa gctgagcggc atctctatgg gctgtgactg
    49801 ctgttacacc aaccacgctg atgctgacca gaacctcaac gaaaacctga tgatcctgct
    49861 cgccaccgca ggctgcaact acatcatggg gatgccgctg ggcgatgaca tcatgctcaa
    49921 ctatcagacc accgcattcc acgacactgc cactgtgcgt cagttactca acctgcgccc
    49981 gtcaccggag tttgaacgct ggctggaaag catgggcatt atggcaaacg gtcgcctgac
    50041 caaacgggcg ggcgatccgt cactgttctt ctgatgacgc ggagataaca catcatggat
    50101 caaaaacaga ttgaagaaat tgtacgcagc gtgatggcgt caatgggaca agcggccccc
    50161 gcgccgtcag aagcaaagtg cgccaccact aactgtgcgg caccggtgac ctcggaaagc
    50221 tgcgcgctgg atttaggttc tgcggaagca aaagcgtgga ttggtgttga aaacccgcat
    50281 cgcgcagacg tattaacaga actgcgccgc agcaccgtgg cccgcgtttg taccggtcgt
    50341 accggtccgc gtccacgtac gcaggcgctg ctgcgtttcc tggccgatca ctctcgttcg
    50401 aaagataccg tactgaaaga agtgccggaa gagtgggtga aagcgcaggg cttgctggaa
    50461 gtacgctccg aaattagcga caaaaatctc tacctgactc gcccggatat gggccgccgc
    50521 ctgtgtgcag aagctgttga agcgctgaaa gcacagtgtg ttgctaatcc ggacgtacag
    50581 gtcgttattt ctgatggcct gtcaactgat gccatcaccg tgaactacga agagatcctg
    50641 ccgccgctga tggcgggcct gaaacaggcc gggctgaaag tcggcacgcc attctttgtt
    50701 cgttatggtc gcgtgaagat tgaagatcag atcggtgaga tcctcggcgc gaaagtggtg
    50761 atcctgctgg tgggggaacg tccggggttg ggtcagtcag aaagcctctc ctgttacgcc
    50821 gtttactcgc cgcgtatggc gaccaccgtt gaggccgatc gcacctgcat ctctaacatt
    50881 caccagggcg gcacgccgcc ggttgaagcc gcagccgtca ttgtggattt ggcgaaacgt
    50941 atgctggagc agaaagcatc cggcatcaac atgacccgat aaggaggcat catgccagct
    51001 ttagatttga ttcgaccgtc ggtaaccgct atgcgggtga ttgcctccgt taacgctgaa
    51061 tttgcgcgtg aactaaaatt gccgccgcat attcgtagcc tcgggctgat ttctgctgat
    51121 tccgatgacg tcacctacat tgcggctgac gaagcgacca agcaggcgat ggtcgaagtg
    51181 gtatatggcc gctcgctgta tgcgggagca gcacacggac cgtcaccgac tgccggtgaa
    51241 gtgctgatta tgcttggtgg gccaaacccg gcggaagtgc gtgctggtct ggatgcgatg
    51301 gttgcgcata ttgaaaatgg cgctgctttc cagtgggcta atgacgcaga aaacaccgca
    51361 ttcctggcac atgtagtttc gcgtaccggt tcttatcttt catcaaccgc cgggatcacg
    51421 cttggcgatc cgatggcgta tctggtggca ccgccgctgg aagcgaccta cggcattgat
    51481 gcagcgttga aatctgccga cgtacagctg gtgacctatg tcccgccacc gtctgaaacc
    51541 aactactcgg cagcattttt aaccggtagc caggccgcgt gtaaagcagc ctgtaacgcc
    51601 tttaccgatg cagtgctgga aatcgcgcgt aatccaatcc agcgtgcgta acggaggttg
    51661 ccgatgatca atgcactggg attgctggaa gtggacggca tggtcgccgc gatagatgca
    51721 gcggatgcca tgctcaaagc agctaacgtt cgtctgctca gtcacgaagt gcttgaccct
    51781 ggtcgcttaa cgctggtggt ggaaggcgat ctggcggcgt gtcgtgcggc gctggacgca
    51841 ggttgtgctg ccgcgatgcg taccgggcgt gtcatcagcc gcaaggagat cggtcggcca
    51901 gacgatgaca cccagtggct ggttactggc tttaaccgcc agccgaagca acccgtaaag
    51961 gaacccgacg cgccagttat cgtcgcggaa tctgctgacg agttgttggc gctgttaaca
    52021 tcagtacgtc agggaatgac ggcaggagaa gtggctgccc actttggctg gccgctggaa
    52081 aaagccagaa atgcgctcga acagctcttt tctgccggga cgttacgtaa acgcagtagt
    52141 cgttatcgtc tcaagcccca ttaacctgtc ggaggtgccg ggtgtcctac aacacccggc
    52201 attaacatca tgaaaaagac ccgtacagcc aatttgcacc atctttatca tgaaccctta
    52261 cccgaaaacc tgaagctcac gccgaaggtc gaagtggata atgttcatca acgacagaca
    52321 acggatgtct atgaacatgc tttgacgatt accgcctggc agcagattta cgatcagctg
    52381 catccgggca agtttcatgg tgaatttacg gaaattctgc tcgatgatat tcaggttttt
    52441 cgtgaataca ccggtctggc gctgcgtcag tcgtgcctgg tctggccgaa ctcgttctgg
    52501 tttggcattc cggcgacgcg cggtgagcag ggatttatcg gttcgcaatg tctgggaagc
    52561 gcagaaatag cgacgcgccc cggtggaacc gagtttgaat taagtacgcc ggacgattat
    52621 acgatcctgg gcgtagtgct gtctgaagat gtcatcaccc gtcaggctaa ctttttgcac
    52681 aatccggatc gggtattaca tatgctgcgt agccagtcgg cgctggaagt gaaagagcag
    52741 cataaagccg cgctgtgggg ctttgtccaa caggcgctgg cgacgttttg cgagaacccg
    52801 gaaaatctcc atcagcctgc agtgcgaaaa gtgctggggg ataatttgct aatggcgatg
    52861 ggggcgatgc tggaagacgc gcaaccaatg gtgacggcgg aaagcatcag tcatcagagt
    52921 taccgtagat tactttcccg cgcccgtgaa tatgtgctgg aaaatatgtc tgagctggtg
    52981 acggtgctgg acttgtgtaa tcaactgcat gtcagtcgcc gcacgctaca aaacgcgttt
    53041 cacgctattt taggcattgg cccaaacgcg tggctgaaac gcattcgcct gaacgccgta
    53101 cgccgcgaac tgataagccc gtggtcgcaa agcacaacgg taaaagacgc cgccatgcag
    53161 tggggattct ggcatctggg gcaatttgcc acggattacc agcagctgtt tgccgagaag
    53221 ccgtcgttga cgttgcatca gcggatgcgg gaatgggggt gagggagtta cacccaatcc
    53281 ctgaccttaa taaactcact taacgccgct tctgggctgc catcttttgg ctgataatca
    53341 tactcccagc gtaccagtgg cggcattgac atcaggatcg actcggtgcg cccgccagtt
    53401 tgcaggccaa acagagtgcc gcgatcccag accagattga actcgacata acgaccgcga
    53461 cggtagagct gaaaattgcg ctcgcgctcg ccgtaggcca tcgctttacg tcgctctaca
    53521 attggtaaat aagcgtcggt gtagcctttg cctaccgcct gcataaaggc aaaacagtgg
    53581 tcgaaatctg gcgtgttcag atcatcaaag aacagcccgc caataccgcg ctgttcgttg
    53641 cgatgtttga ggtagaagta atcgtcgcac cactttttgt aacggggata aacgtcttca
    53701 ccaaatggca ggcacaggtc acgggcggtg cggtgccagt gaatggcgtc ttcttcaaaa
    53761 ccatagaaag gggttaaatc gaagccgccg ccaaaccacc agacgggctc tgcacccggt
    53821 ttttcggcaa taaaaaaacg cacattcgcg tggctggtgg gaacatacgg gttatgcgga
    53881 tgcactacca gtgaaacgcc catcgcctcg aaactgcgcc cggcaagttc cgggcgatga
    53941 gcggtggcgg aagcaggcat cgcctcgccg tggacgtgag aaaagttgac gcctgcctgt
    54001 tcgaaaacac caccattacg caacacccga ctacgcccgc cgccgccagc ttcgcgctgc
    54061 caactatctt cgacaaattc tgcgccatcg acggcggtca gctgctgaca aatcgtatcc
    54121 tgaaggttga gcagaaactg tttaacctgg tgtgcgtcgg gtttcataac ttatcgcttt
    54181 ttcgaatgtg ctttctggtt gtcgaaccag tggaaataac tgatcacgcc ttcagcaatc
    54241 gctgtggcga ttttctgacg aaacgccgcc gtgcctaaca gccgctcttc ttccgggttg
    54301 gtgataaacg aggtttccac cagcaccgaa ggaaccgacg gcgatttcaa caccacaaac
    54361 gcggcttgtt cggtgttgcg gctgtgcagt ttatgcaccg gcttaatctt tttcagaata
    54421 tgcgagccaa gcgtcaggct gtttttaata gtatcggttt gcaccagatc aaacagcact
    54481 tgctgcaata ggtgatcctt gtcagtcgcc tttttaccgg caacttcatc ggcgcggttt
    54541 tcgcgttcag acaggtattt cgccattgcg ctacttgccc cacggttaga gagggcaaat
    54601 accgaagcac cggcagcttt cgggttggta aagccatcgg catgaattga cataaacaga
    54661 tctgcgccat gtttatgggc gatttcaacg cgatcgtaaa gtgggataaa cgtatcgcca
    54721 gaacgcgtta aacgcgcatc aatcccatga ttacgcaaaa tggaacggac gtttttagca
    54781 atcgccagca ccacatgttt ttctttcgaa ccgttgcgtc cgatcgctcc ggtatcaatt
    54841 ccgccgtggc ctggatcgag aacaacgaca cgtttgccgc cagatttttt ggctttcggc
    54901 ttgctgtgtc cgttgctggt ttttaaaggt tcgtctttgg cgatggcttg cgacattcct
    54961 gacaacgtca gggcagccaa accggctttc agcacctggc ggcgcgaagt gagtgttttt
    55021 agtggtttaa aagtgctcat acggcctgag ttgtaataat aaagttccag atgttatatc
    55081 gtatcgcgta atccgttacg acggtttgat gttgagaatt gttttacttt tcatttcaat
    55141 agttgacaac tccccattat gccacttttt caaccgattt tcgtcagata ttggctaatt
    55201 cgaccgttca cgccataatc agccttttaa cccgcaaaaa ggtaacgaat accatggaga
    55261 tacgcgtatt tcgccaggaa gatttcgaag aggtcatcac cctttgggag cgttgcgact
    55321 tgctgcgtcc gtggaacgat ccggaaatgg acatcgagcg taagatgaac catgacgtca
    55381 gtttgttttt ggtcgctgag gtaaacggtg aagtggtcgg aacggtgatg ggcggttatg
    55441 acgggcatcg cgggtctgct tattatcttg gcgtgcatcc agagtttcgt gggcgtggga
    55501 ttgccaatgc gttgcttaat cggctggaga aaaagctgat tgctcgtggc tgcccgaaaa
    55561 ttcagatcaa tgtgccggaa gataacgaca tggtgctcgg aatgtatgaa cgcctgggat
    55621 atgaacacgc cgacgtgctg agtctgggta agcgtttgat tgaagatgaa gagtactgag
    55681 ttccatcctg tccattatga tgcgcacgga cgcctgcgtt tacccttgct cttctggctt
    55741 gtgctattgc ttcaggcgcg aacctgggtg ctgtttgtca ttgccggtgc gtcgcgcgag
    55801 cagggtactg cgctgctaaa tctgttttat cccgatcacg ataatttctg gctggggctg
    55861 attccaggca ttccggcggt gctggcgttt ttgttgagcg gtcggcgggc tacatttcct
    55921 cgcacttggc atgtgctcta ttttctgttg ctgttggcgc aggtggtttt actttgctgg
    55981 caaccgtggc tgtggctgaa cggtgaatcc gttagcggta tcggtctggc gctggtagtg
    56041 gcggatattg tggcgttaat ctggctgctg accaatcgac gtttacgcgc ttgttttaat
    56101 gaagaaaaag aataacggca ctttttggtg aatttgcact ccaagcaacg ttattgaata
    56161 accaaaggaa gtgacatgaa atcgctgcgt ttaatgttat gcgctatgcc gttgatgctg
    56221 accggctgtt ccacgatgtc gtcagttaac tggtcggccg ctaacccgtg gaactggttt
    56281 ggatcgtcca ccaaagtgag cgagcagggc gtgggtgaat taacggcgtc cacaccactg
    56341 caagaacaag ccattgctga tgcgcttgat ggcgattatc gcctgcgcag cggaatgaaa
    56401 accgcgaacg gcaacgtggt gcgctttttt gaagtgatga aaggcgacaa cgtggcgatg
    56461 gtgattaacg gcgatcaagg cacgatcagt cgcattgacg tgctggatag cgatattcct
    56521 gctgacaccg gtgttaaaat cggtacaccg tttagcgacc tttacagcaa agcatttggc
    56581 aattgccaaa aagctgatgg tgatgataat cgtgccgtcg aatgtaaagc cgaaggcagt
    56641 caacatatta gctaccagtt cagcggggaa tggcgtggtc ctgaagggtt aatgccttcg
    56701 gacgataccc tgaaaaactg gaaagtcagt aaaattatct ggcggcgtta atttgcgtct
    56761 gaacaaaccg ccgccgcgaa aaaacacgta aaatagcgct caacaatgcc acggattgcg
    56821 tggcattctt attttcagga ggaacaatgt ctcaggttca gagtggcatt ttgccagaac
    56881 attgccgcgc ggcgatttgg atcgaagcca acgtgaaagg ggaagttgac gccctgcgtg
    56941 cggccagtaa aacatttgcc gacaaactgg caacttttga agcgaaattc ccggacgcgc
    57001 atcttggtgc ggtggttgcc tttggtaaca acacctggcg cgctctgagc ggcggcgttg
    57061 gggcagaaga gctgaaagat tttccgggct acggtaaagg ccttgcgccg accacccagt
    57121 tcgatgtgtt gatccacatt ctttctctgc gtcacgacgt aaacttctct gtcgcccagg
    57181 cggcgatgga agcctttggt gactgcattg aagtgaaaga agagatccac ggcttccgtt
    57241 gggtggaaga gcgtgacctg agcggctttg ttgacggtac agaaaacccg gcgggtgaag
    57301 agacgcgtcg cgaagtggcg gttatcaaag acggcgtgga tgccggcggc agctatgtgt
    57361 ttgtgcagcg ttgggagcac aacctgaagc agctcaaccg gatgagcgtt cacgatcagg
    57421 agatgatgat cgggcgcacc aaagaggcca acgaagagat tgacggcgac gaacgtccgg
    57481 aaacctctca cctcacccgc gttgatctga aagaagatgg caaagggctg aagattgttc
    57541 gccagagcct gccgtacggc accgccagtg gcactcacgg tctgtacttc tgcgcctact
    57601 gcgcgcgtct gcataacatt gagcagcaac tgctgagcat gtttggcgat accgatggta
    57661 agcgtgatgc gatgttgcgt ttcaccaaac cggtaaccgg cggctattac ttcgcgccgt
    57721 cgctggacaa gttaatggcg ttgtaagcgt ttctgattcg atgattaaag gccagccagt
    57781 aacaaaactg actggccttt ttattacttt tgcttcaacg ccgcatacac ctgatcaact
    57841 acccaaccat aagttgcaat cggtaactga ccgctttcga acatattggg atttttttgt
    57901 ggatcggcaa ctggcgaatg gggcttgttg cttaacatca caatcgccat atgattgacc
    57961 ggatcgataa cggttactgt cccggtccag ccagtgtgac cgtaggtttg cgggcttgcc
    58021 agcgtgccaa acgtcggcgt catggtggca ttaccattca cgcgccagcc gaggccaaaa
    58081 gtggcatctt ccttagagct ggtggtgaac atcttcaccg tttccgcact gaacagctgc
    58141 acatcgccat agcccccgcc gttcagcatc gtttgcatta acaccgcaat atcgccggta
    58201 ttggaaaaca aacctgcgtg cccggaaacg ccgcccatcg aataaaaggc tttttcatcg
    58261 tgcacctgac cccagagagt ggaggtgcgg atattcggga aatggatcac gccatcgcgg
    58321 gtattgccgt tcaattccgt ggcggcaatt tgttgcggtt taaagccttt cagcagcggg
    58381 ttaaataccg tatgtgtcag gccgagcggg cgataaatcg actcttcaac atagcggtca
    58441 agcggctgac cggtaaccga ctcgacgata aatccaagca gcatataatc gacatcgctg
    58501 tagatatgtt tgctgccggg ctgatattcc agcggcgtgc gcttgatcat ctccagcgtc
    58561 tggcctttat cctgggaata taacgcgccc gcgaccgctt tattcgggta ttgcggatct
    58621 gccgggaaac cgccgctgtg atgcagcagg tcagaaatcc gcagggtgtt tttgccttta
    58681 atggtgtcat ttggactatc ggcaaacccc ggaatatatt tcgcaatccg atcgtcagga
    58741 tgcagtttgc cttcgctcat cagcttttgc agggcgaagt tcgtggcgta cattttggtg
    58801 tttgaggcca ggtcatacag cgtcccggtg gtggctttga caggctgttc catcaacacg
    58861 ctgccatcgt actttttcgc cgctccccag gctttgcgat acacaatctg attatcttta
    58921 atgatcagca ggtttacgcc gggataaccg acatcaattt gctggctaat ccagcgatcc
    58981 atctggttaa gccgttcgac gttaaaccct gctttctctg gcgagctttc tgtcagaaca
    59041 gggtattttg cggcgctgac gctacaacta acagccagca gagaaagata gagcattgtc
    59101 cgtttcatga tatcagtcca gattgacgtt acggcagcca aagagcgtgg tgaaaataaa
    59161 cccgcaaacc catgccacca gaatcccgcc agcataaacc gccatggccg gaaggatgcc
    59221 ttgtgcggaa gtcatcagcg gcagcgctac cagaccagac ggcccaaagg cgctgtttaa
    59281 gcccatcggc agaccccatc aggctatcag gccgataaac aaaccgcccg ccgcgccgcc
    59341 taaacaggcg gtaacaaacg gcttcatgcg gggcagggtg acaccgtaaa tcagcggttc
    59401 accaacgccc agcaggccgg gaataatcgc cccgcgtacc tgactgcgta gcgcactgtg
    59461 cggttgcgcc cgccagtaga gtgccagcgc cgcgcccacc tggcccgcgc ctgccattga
    59521 aaggatggga aataagctgt tgaatcccta gctgtccatt aacgcgaggt aaacaggaat
    59581 aaagccctga tgcacgccaa ataccacggc gatcaggaac agcccggcta aaaccgcaca
    59641 accgaacgga ttactgttca ggtgcataaa cagccatgac ataccttcga ataaccagcc
    59701 acccagcggc ataatgatca ggtacgcaag cgtagcggtg atcagcaggg tgattaacga
    59761 ggtcagcagc atgtcgagat cgtccgacat aaagcggcgc accatgcctt caatgcgggc
    59821 gcaggcccac gcggcaatca gcacgccgat aatattgccg cgcggatcga tgggcagacc
    59881 aaagaaatcg tgaaaaccgg cgtagtcacc ggtggttgca gcggggttat aaccgagcaa
    59941 aaagagtgcg gcaataattg cgccattgac gcccgtgccg ccaaatgcct gggcggcgtt
    60001 atagcccacc agaatcacca ggaaagtgaa caaacctttg ctgaacacct tcataaaatt
    60061 cagcgcgtcg ggtagcgtgc cctgagcatc tgccggaacg tgcatcaccg tggcaattaa
    60121 cgtcgctatc cccagcagca gaccggcggc aataaaaccg gggatcagcg gcgtgaagat
    60181 ggtggcgaat ttggcgagaa attgttgcac gccggaggtt tgtttggctt ttaactgacg
    60241 tttgttctgg gcggcgattt ctgcggcatc ctgtaccggt gcctcgccca gcagctcgct
    60301 catcgctttt gcggcacgat gcgctttacc aggcccaaaa acgacctgca cctggtcgct
    60361 ggtcaaaatg acgcccttca cgccttccag cgttttgata ttgggatcaa ccagtgaact
    60421 gtcatgtaca cccagacgca ggcgcgtcat acagttacca caactggcga tatttcccag
    60481 tccgccgaca cgggtaagaa tggtgttcag aagttcactg ctgatctctt tggccatggg
    60541 ttattccttg tctaaaacct gacgaataaa gccgccgtgt tgatccaggc gttttttagc
    60601 ttctgcggca tcgagatttt tcagcaccat cacaatggcc gttttacagt tgcgctcgca
    60661 ggcaattaac gccgcttccg cttgttcggc attacatccg gtggcgtttt taacgatatt
    60721 gacctggcgc acatgcagtt tttcgttggt ggcgaccaca tcgaccatca ggttgccgaa
    60781 cactttgccg gatttaatca tcagcccggt ggaaagcata ttgagcacca gtttctgcgc
    60841 tgtgcctgct ttcattcgcg aagaaccagt gaccacttcg gcacctacaa tcggtgtaat
    60901 ggcaaactcg gcggtggtgg aaacggcact ccccggatta caggaaattc ccactgtgcg
    60961 gcaaccgagc tggcgggcgt attccagtcc ggcaatcaca tacggcgtac gaccgctggc
    61021 ggcaataccg accaccacat cctgtgccgt taaatttata tttttcagat cgttaatgcc
    61081 gccttctcgg ctatcttccg tgccttccac cgcgtgctga atggcatatt cgccgccagc
    61141 aatcaaacca accaccagac ccggtttcac gccgtaggtg ggcggacatt cgctggcatc
    61201 cagaatcccc agacgaccgg atgttcccgc accgagataa atcagacgac cgccgccgct
    61261 tacctgggcg tggataacat cgatcgccgc ggcgatatcc ggcaggacgc gctcaacggc
    61321 aagcggtacg gttttatctt catcgttgat aatccggcac atttccagcg tcgatacgcg
    61381 gtcaatttca gccgaggcgg tgttcgagcc ttcagtaatc atcttttcaa gttgcatcgg
    61441 tggtgacctt actatttacg atcaaataat tgcagatgta tggaatcata tatcccgaat
    61501 tgacagactt aaaggaatca ttgagtctac acttgcagag tgtgaagagc gtcataagaa
    61561 cttaagcgga atttttgctg ccagcccggg agagtggaag atgttgtacc tgacgaaaat
    61621 acgcaatgcg gaaagtgaat ttaccgagaa cgagcaaaaa attgctgact ttctgcgggc
    61681 caacgtgagt gagctgaaat ccgtctcttc gcgcaagatg gcgaaacagc tcggcatcag
    61741 tcagtcgagc attgtgaaat ttgcccagaa gctcggcgcg caaggattta ctgaattgcg
    61801 tatggcgtta attggcgaat acagtgccag ccgggagaaa acgaatgcca cggcgctgca
    61861 cctgcacagt tcgattacca gtgatgattc gctggaagtg attgcccgca aactgaatcg
    61921 cgaaaaagag ctggcgctgg agcaaacctg tgcgttgttc gactacgctc ggctgcaaaa
    61981 aatcattgag gtcatcagta aagcaccgtt tatccagata acgggcctgg gcgggtcggc
    62041 gctggtgggg cgcgatctgt cgttcaaact gatgaaaatt ggctatcgcg tggcctatga
    62101 agccgacaca cacgtacaag ccaccgtttc ccaggcgttg aaaaaaggcg atgtacaaat
    62161 cgctatctct tacagcggca gtaaaaaaga gattgtcttg tgcgcagaag cggcacgaaa
    62221 gcagggggca acggtgattg ccattacttc tctggctgac tctccgctgc ggcggctggc
    62281 gcatttcacg ctcgataccg tttccggtga aaccgaatgg cgtagctcgt cgatgtctac
    62341 ccgcacggcg caaaattctg ttaccgattt actgtttgtt ggtcttgtgc aactcaatga
    62401 tgtggagtca ttgaaaatga ttcagcgcag cagtgagcta acacaacgcc tgaaatgatg
    62461 cataaagcag cgactggatt gagattttcc tgaattagtg agctgatccg cagcaatatt
    62521 ttgtttatcc tgtattttca gagggaatgg agtgtaacgc tctgtattaa caaggagagc
    62581 attaaaatgg gtaaactcac gggcaagaca gcactgatta cgggcgcatt gcagggaatt
    62641 ggcgaaggaa ttgccagaac ttttgcgcgt catggcgcaa acctaatctt gctggatatc
    62701 tcccctgaga tcgaaaagct ggcggacgaa ctgtgtggtc gtggtcatcg ctgtacggcg
    62761 gttgtcgccg atgtgcgtga cccggcgtcg gtagccgcag ctatcaaacg cgcgaaggaa
    62821 aaagaagggc gcattgatat cctggtgaat aacgcaggcg tttgtcgtct gggcagtttc
    62881 ctcgatatga gcgatgacga tcgcgatttc catattgaca tcaatattaa aggcgtatgg
    62941 aacgtcacga aggcggtgct gccggagatg attgcccgca aagatggtcg cattgtgatg
    63001 atgtcttcag tcactggtga tatggtggcc gatcctggcg aaacggcgta cgccttaacg
    63061 aaagcggcga ttgttggcct gacaaaatcg ctggcggtgg agtacgcgca gtctggtatt
    63121 cgcgttaacg ccatttgccc gggatacgtg cgcacaccaa tggcggaaag cattgcccgc
    63181 cagtcgaacc cggaagatcc agagtcggtg ctgactgaaa tggcgaaagc aatcccgctg
    63241 cgtcgcctcg ccgatccgct ggaagtcggc gaactggcgg ccttcctcgc atcggatgaa
    63301 tccagctatt taaccggtac acagaatgtg attgatggcg gcagcacact gccggagacg
    63361 gttagcgtcg gtatctgatt cacctctgtt tcctccctgc atttgtgggg aggatttcgt
    63421 tttaaacccg gttcttattc ccttttcaac ttccaaatca ccaaacggta tataaaaccg
    63481 ttactccttt cacgtccgtt ataaatatga tggctattag aaagtcatta aatttataag
    63541 ggtgcgcaat ggccgttaac ttactgaaaa agaactcact cgcgctggtc gcttctctgc
    63601 tgctggcggg ccatgtacag gcaacggaac tgctgaacag ttcttatgac gtctcccgcg
    63661 agctgtttgc cgccctgaac ccgccgtttg agcaacaatg gaccaaagaa aacggcggcg
    63721 acaaactgac aataaaacaa tctcatgccg ggtcatcaaa acaggcgctg gcaattttgc
    63781 agggcttaaa agcagacgtt gtcacttata accaggtgac tgacgtacaa atcctgcatg
    63841 acaaaggcaa gctgatcccg gcggactggc aaacgcgcct gccaaataat agctcgccat
    63901 tctactccac catgggcttc ctggtgcgta agggcaaccc gaagaatatc cacgactgga
    63961 acgacctggt gcgctccgat gtgaagctga ttttcccgaa cccgaaaacg tcgggtaacg
    64021 cgcgttacac ctatctggcg gcatggggcg cagcggataa agctgacggt ggcgataaag
    64081 ccaaaaccga acagtttatg acccagttcc tgaaaaacgt tgaagtgttc gatactggcg
    64141 gtcgtggcgc gaccaccact tttgccgagc gcggcctggg cgatgtgctg atcagcttcg
    64201 agtcggaagt gaacaacatc cgtaaacagt atgaagcgca gggttttgaa gtggtgattc
    64261 cgaaaaccaa cattctggcg gaattcccgg tggcgtgggt tgataaaaac gtgcaggcca
    64321 acggtacgga aaaagccgcc aaagcctacc ttaactggct ctacagcccg caggcgcaaa
    64381 ccatcatcac cgactattac taccgcgtga ataacccgga agtcatggat aaactgaaag
    64441 acaaattccc gcagaccgag ctgttccgcg tggaagacaa atttggctcc tggccggaag
    64501 tgatgaaaac ccacttcacc agcggcggcg agttagacaa gctgttagcg gcggggcgta
    64561 attaatgttt gcagtttcat caagacgcgt gctgccgggc tttaccttaa gcctcggcac
    64621 cagtctgctg tttgtgtgcc tgattttgct gctgccgctc tccgcgctgg tgatgcagct
    64681 ggctcagatg agctgggcgc agtactggga ggtgatcacc aacccgcagg tagtagcggc
    64741 ctacaaagta acgctgctgt cagcgtttgt ggcatcgatt tttaacggcg tttttggcct
    64801 gctgatggcg tggatcttaa cccgctatcg ctttccgggc cgcacgctgc ttgatgcgct
    64861 gatggattta ccctttgcgc tgccaacggc agtcgccggt ttaacgctag cctcgctctt
    64921 ttctgtaaac ggtttttacg gtgaatggct ggcgaagttt gatatcaaag tcacctatac
    64981 ctggctgggg attgcggtgg cgatggcctt taccagcatt ccgtttgtgg tacgtaccgt
    65041 gcagccggtg ctggaagagt taggcccgga atatgaagaa gcggcagaaa cgcttggcgc
    65101 aacgcgctgg cagagtttct gcaaagtggt gctgccggaa ctttctccgg cgctggtggc
    65161 gggcgtggcg ctgtcgttta cccgtagtct tggtgaattt ggcgcggtga tttttatcgc
    65221 cggaaatatc gcgtggaaga cggaagtgac gtcgctgatg atttttgtgc gcttacagga
    65281 gtttgattac ccggcagcga gcgcgattgc ttcggtgatc ctcgcggcat ctctgctgct
    65341 gctgttctca attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag gtcattaatg
    65401 gcggaagtta cccaattgaa gcgttatgac gcgcgcccga ttaactgggg caaatggttt
    65461 ctgattggca tcgggatgct ggtatcggcg ttcatcctgc tggtgccgat gatttatatc
    65521 ttcgtacagg cgttcagcaa agggctgatg ccggttttac agaatctggc cgatccggac
    65581 atgctgcacg ccatctggct gacggtgatg atcgcgctga ttgccgtacc ggtaaacctg
    65641 gtgttcggca ttctgctggc ctggctggtg acgcgcttta acttccctgg acgccagtta
    65701 ctgctgacgc tactggacat tccgtttgcc gtatcgccgg tggttgccgg tctggtgtat
    65761 ctgctgttct acggctctaa cggcccgctg ggcggctggc tggatgaaca taatctgcaa
    65821 attatgttct cctggcccgg aatggtgctg gtcaccatct ttgtgacgtg tccgtttgtg
    65881 gtgcgcgagc tggtgccggt gatgttaagc cagggcagcc aggaagacga agcggcgatt
    65941 ttgcttggcg cgtctggctg gcagatgttt cgccgcgtca cattgccgaa cattcgctgg
    66001 gcgctgcttt atggcgtggt gttgaccaac gcccgcgcaa ttggcgagtt tggcgcggtg
    66061 tcggtggttt ccggctcgat tcgcggcgaa accctgtcgc tgccgttaca gattgaattg
    66121 ctggagcagg actacaacac cgtcggctcc tttaccgctg cggcactgtt aacgctgatg
    66181 gcgattatca ccctgttttt aaaaagtatg ttgcagtggc gcctggagaa tcaggaaaaa
    66241 cgcgcacagc aggaggaaca tcatgagcat tgagattgcc aatattaaga agtcgtttgg
    66301 tcgtacccag gtgctgaacg atatctcact ggatattcct tcaggtcaga tggtcgcgtt
    66361 gctggggccg tccggttccg ggaaaaccac gctgctgcgc attatcgccg ggctggagca
    66421 tcaaaccagc gggcatattc gcttccacgg caccgacgtc agccgcctgc acgcgcgcga
    66481 tcgcaaggtc ggtttcgtgt tccagcatta cgcgctgttc cgccatatga cggtattcga
    66541 caatatcgct tttggcctga cggtgctgcc gcgtcgcgat cgcccgaatg ccgcagccat
    66601 caaagcgaaa gtgacaaaat tgctggagat ggtgcagctt gctcatctgg cggatcgtta
    66661 tccggcacag ctttccggcg gccagaaaca gcgcgtggcg ctggcgcgcg cgctggctgt
    66721 agaaccgcaa attctgctgc ttgatgaacc gtttggcgcg ctggatgcgc aggtgcgtaa
    66781 agagctgcgt cgctggctgc gtcaactgca tgaagagctg aaattcacca gcgtgttcgt
    66841 gacccacgac caggaagaag cgacggaagt agctgatcgt gtagtggtga tgagccaggg
    66901 caatattgaa caggctgatg cgccggagca ggtatggcgc gaaccagcga cccgttttgt
    66961 gctcgaattt atgggcgaag tgaaccgcct gcagggaacc attcgcggcg ggcagttcca
    67021 tgttggcgca catcgctggc cgctgggcta cacacctgct tatcaggggc cggtggatct
    67081 cttcctgcgc ccgtgggaag tggatatcag ccgccgtacc agtctcgatt cgccgctgcc
    67141 ggtacaggta ctggaagcca gcccgaaagg tcactacacc caattagtgg tgcagccgct
    67201 ggggtggtac aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc cgcagcgtgg
    67261 cgagcgttta ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg agcgtatcga
    67321 aacccgcgat gaggaacttg ctctcgcaca aagcgcctga taggttgagt gaatgttaaa
    67381 cgcccggagg cgcttcccgc gatccgggct ttttaatggc aaggtttgta acctgtagac
    67441 ctgataagac gcgcaagcgt cgcatcaggc aacaccacgt atggatagag atcgtgagta
    67501 cattagaaca aacaataggc aatacgcctc tggtgaagtt gcagcgaatg gggccggata
    67561 acggcagtga agtgtggtta aaactggaag gcaataaccc ggcaggttcg gtgaaagatc
    67621 gtgcggcact ttcgatgatc gtcgaggcgg aaaagcgcgg ggaaattaaa ccgggtgatg
    67681 tcttaatcga agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gccgcgctga
    67741 aaggctatcg catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga
    67801 tgcgtgctta tggtgcggaa ctgattcttg tcaccaaaga gcagggcatg gaaggtgcgc
    67861 gcgatctggc gctggagatg gcgaatcgtg gcgaaggaaa gctgctcgat cagttcaata
    67921 atcccgataa cccttacgcc cattacacca ctaccgggcc ggaaatctgg cagcaaaccg
    67981 gcgggcgcat tacccatttt gtctccagca tgggaacaac cggcactatc accggcgttt
    68041 cacgctttat gcgcgaacaa tccaaaccag taacgattgt tggcctgcag ccggaagaag
    68101 gcagcagtat tcccggcatt cgccgctggc ctgcggaata tctgccgggg attttcaacg
    68161 cttctctggt ggatgaggtg ctggatattc atcagcgcga tgcagaaaac accatgcgcg
    68221 aactggcagt gcgggaagga atattctgtg gcgtcagctc cggcggcgcg gttgccggag
    68281 cactgcgggt ggcaaaagct aaccctggtg cggtggtggt agcgatcatc tgcgatcgtg
    68341 gcgatcgcta cctttccacc ggggtgtttg gggaagagca ttttagtcag ggggcgggga
    68401 tttaagatta ttgcgcaggc accgttttat ccgtctgcgc aattaaggca tgtaaaaaag
    68461 gctgtgcgtt ctcatcgctc ttaccgggga tcttcacaat atacggcttc ccggtaatcg
    68521 atgacgacga agcaacctta tcaataaact gctcggcagt gtcaatgcgg ttacgggtat
    68581 tgccgagctt cagacgcaga tgagaaaccg cttcatcgca ggtatgttca tcaccgttgc
    68641 gcacaaatat caaatccttt ttctgtgcta atccctccag catggcgttg atgcgggctt
    68701 cttcatgaac agttaacttc gcgtaagcgg ggagtgtcat tagtagtgta atgaccagac
    68761 aaatgatttt cttcattgaa ttaccttatc cttgttttgt gatagccgat ttgactgcca
    68821 gaatcaggta aagtttcggt tggaatacga tgaggatcat cggcaaggag aaaaaattaa
    68881 atgagtagtt tgttgttgtt taacgataag agtagggcgc tgcaggcgga tatcgtcgcc
    68941 gtgcagtcgc aggtagtgta cggcagcgta ggcaacagca ttgcggtgcc tgctataaaa
    69001 cagaacggct tgaatgtctt tgccgtgccg acggtattgc tgagcaatac gccgcattat
    69061 gacactttct acggtggtgc gattccggac gaatggttta gcggctattt gcgtgcgctt
    69121 caggagcgtg atgcgctgcg ccaacttcgt gctgtaacca cgggctatat gggaacggca
    69181 tcgcaaatca aaatccttgc cgagtggctg actgcgctac gcaaagacca tcctgaccta
    69241 ttgatcatgg tcgatccggt gattggcgat attgatagcg gaatttatgt caaacctgac
    69301 cttcccgagg cgtatcgaca atatttactg ccgctggcgc aaggaattac ccccaatatc
    69361 tttgagctgg aaatcctgac cggtaaaaat tgccgcgatc tcgacagtgc cattgctgcc
    69421 gcaaaaagtc tgctttcaga cacattaaaa tgggtggtga ttaccagcgc ctccggtaat
    69481 gaagaaaatc aggagatgca ggtggtggtg gtcagtgccg acagcgtgaa tgtcatttcc
    69541 cattcacggg taaaaaccga cctgaaaggg actggcgacc tgttttgtgc tcagcttatc
    69601 agtggtttgc tgaaagggaa ggcgttaaac gatgcggtgc accgtgcggg gttgcgcgta
    69661 ctggaagtga tgcgctatac ccagcagcat gagagcgatg aattgatttt gccgccgctg
    69721 gcggaagcat aaaaaatggc gccgatgg
//
LOCUS       DAJTVE010000005        62988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000005 DAJTVE010000000
VERSION     DAJTVE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..415)
                     /locus_tag="OO439_000306"
                     /pseudo
     CDS             complement(1..415)
                     /locus_tag="OO439_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(521..3082)
                     /gene="mutS"
                     /locus_tag="OO439_000307"
     CDS             complement(521..3082)
                     /gene="mutS"
                     /locus_tag="OO439_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6314780.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3158..3292
                     /locus_tag="OO439_000308"
     CDS             3158..3292
                     /locus_tag="OO439_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314781.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3369..3722
                     /locus_tag="OO439_000309"
     CDS             3369..3722
                     /locus_tag="OO439_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6314782.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="OO439_000310"
                     /pseudo
     CDS             complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="OO439_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="OO439_000311"
     CDS             complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="OO439_000311"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6314783.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="OO439_000312"
     CDS             complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="OO439_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6314784.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="OO439_000313"
     CDS             complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="OO439_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6314785.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="OO439_000314"
     CDS             complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="OO439_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6314786.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="OO439_000315"
     CDS             complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="OO439_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6314787.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9739..10200
                     /gene="hycA"
                     /locus_tag="OO439_000316"
     CDS             9739..10200
                     /gene="hycA"
                     /locus_tag="OO439_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6314788.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10325..10936
                     /gene="hycB"
                     /locus_tag="OO439_000317"
     CDS             10325..10936
                     /gene="hycB"
                     /locus_tag="OO439_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6314789.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10933..12759
                     /gene="hycC"
                     /locus_tag="OO439_000318"
     CDS             10933..12759
                     /gene="hycC"
                     /locus_tag="OO439_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6314790.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12762..13685
                     /gene="hycD"
                     /locus_tag="OO439_000319"
     CDS             12762..13685
                     /gene="hycD"
                     /locus_tag="OO439_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6314791.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13703..15400
                     /gene="hycE"
                     /locus_tag="OO439_000320"
     CDS             13703..15400
                     /gene="hycE"
                     /locus_tag="OO439_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6314792.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15410..15952
                     /gene="hycF"
                     /locus_tag="OO439_000321"
     CDS             15410..15952
                     /gene="hycF"
                     /locus_tag="OO439_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6314793.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15952..16719
                     /gene="hycG"
                     /locus_tag="OO439_000322"
     CDS             15952..16719
                     /gene="hycG"
                     /locus_tag="OO439_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6314794.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16716..17126
                     /gene="hycH"
                     /locus_tag="OO439_000323"
     CDS             16716..17126
                     /gene="hycH"
                     /locus_tag="OO439_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6314795.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17119..17589
                     /gene="hycI"
                     /locus_tag="OO439_000324"
     CDS             17119..17589
                     /gene="hycI"
                     /locus_tag="OO439_000324"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6314796.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17614..18387
                     /locus_tag="OO439_000325"
     CDS             17614..18387
                     /locus_tag="OO439_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314797.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18549..18860
                     /locus_tag="OO439_000326"
     CDS             18549..18860
                     /locus_tag="OO439_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6314798.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18857..19276
                     /locus_tag="OO439_000327"
     CDS             18857..19276
                     /locus_tag="OO439_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6314799.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="OO439_000328"
     CDS             complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="OO439_000328"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6314800.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="OO439_000329"
     CDS             complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="OO439_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6314801.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22540..23550
                     /gene="ascG"
                     /locus_tag="OO439_000330"
     CDS             22540..23550
                     /gene="ascG"
                     /locus_tag="OO439_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6314802.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23698..24225
                     /gene="hydN"
                     /locus_tag="OO439_000331"
     CDS             23698..24225
                     /gene="hydN"
                     /locus_tag="OO439_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6314803.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24378..26630
                     /gene="hypF"
                     /locus_tag="OO439_000332"
     CDS             24378..26630
                     /gene="hypF"
                     /locus_tag="OO439_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6314804.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26758..27891)
                     /gene="norW"
                     /locus_tag="OO439_000333"
     CDS             complement(26758..27891)
                     /gene="norW"
                     /locus_tag="OO439_000333"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6314805.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27888..29327)
                     /gene="norV"
                     /locus_tag="OO439_000334"
     CDS             complement(27888..29327)
                     /gene="norV"
                     /locus_tag="OO439_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6314806.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29514..31028
                     /gene="norR"
                     /locus_tag="OO439_000335"
     CDS             29514..31028
                     /gene="norR"
                     /locus_tag="OO439_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6314807.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="OO439_000336"
     CDS             complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="OO439_000336"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6314808.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="OO439_000337"
                     /gene_synonym="gutR"
     CDS             complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="OO439_000337"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6314809.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="OO439_000338"
     CDS             complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="OO439_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6314810.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="OO439_000339"
                     /pseudo
     CDS             complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="OO439_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="OO439_000340"
                     /gene_synonym="gutB"
     CDS             complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="OO439_000340"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6314811.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="OO439_000341"
                     /gene_synonym="gutE"
     CDS             complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="OO439_000341"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6314812.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="OO439_000342"
                     /gene_synonym="gutA"
     CDS             complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="OO439_000342"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6314813.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36217..37302
                     /gene="mltB"
                     /locus_tag="OO439_000343"
     CDS             36217..37302
                     /gene="mltB"
                     /locus_tag="OO439_000343"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6314814.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37447..37944
                     /gene="pncC"
                     /locus_tag="OO439_000344"
     CDS             37447..37944
                     /gene="pncC"
                     /locus_tag="OO439_000344"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6314815.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38024..39085
                     /gene="recA"
                     /locus_tag="OO439_000345"
     CDS             38024..39085
                     /gene="recA"
                     /locus_tag="OO439_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6314816.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39313..39813
                     /gene="recX"
                     /locus_tag="OO439_000346"
     CDS             39313..39813
                     /gene="recX"
                     /locus_tag="OO439_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6314817.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39941..42571
                     /gene="alaS"
                     /locus_tag="OO439_000347"
     CDS             39941..42571
                     /gene="alaS"
                     /locus_tag="OO439_000347"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6314818.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42806..42991
                     /gene="csrA"
                     /locus_tag="OO439_000348"
     CDS             42806..42991
                     /gene="csrA"
                     /locus_tag="OO439_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6314819.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43307..43399
                     /locus_tag="OO439_000349"
     tRNA            43307..43399
                     /locus_tag="OO439_000349"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43341..43343,aa:Ser,seq:gct)
     gene            43403..43479
                     /locus_tag="OO439_000350"
     tRNA            43403..43479
                     /locus_tag="OO439_000350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43437..43439,aa:Arg,seq:acg)
     gene            43543..43619
                     /locus_tag="OO439_000351"
     tRNA            43543..43619
                     /locus_tag="OO439_000351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43577..43579,aa:Arg,seq:acg)
     gene            43818..43894
                     /locus_tag="OO439_000352"
     tRNA            43818..43894
                     /locus_tag="OO439_000352"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43852..43854,aa:Arg,seq:acg)
     gene            44175..44741
                     /gene="yqaB"
                     /locus_tag="OO439_000353"
     CDS             44175..44741
                     /gene="yqaB"
                     /locus_tag="OO439_000353"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6314820.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44738..45166
                     /locus_tag="OO439_000354"
     CDS             44738..45166
                     /locus_tag="OO439_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6314821.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45239..46795
                     /gene="gshA"
                     /locus_tag="OO439_000355"
     CDS             45239..46795
                     /gene="gshA"
                     /locus_tag="OO439_000355"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6314822.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46945..47460
                     /gene="luxS"
                     /locus_tag="OO439_000356"
     CDS             46945..47460
                     /gene="luxS"
                     /locus_tag="OO439_000356"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6314823.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47524..49062)
                     /gene="emrB"
                     /locus_tag="OO439_000357"
     CDS             complement(47524..49062)
                     /gene="emrB"
                     /locus_tag="OO439_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6314824.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49079..50251)
                     /gene="emrA"
                     /locus_tag="OO439_000358"
     CDS             complement(49079..50251)
                     /gene="emrA"
                     /locus_tag="OO439_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6314825.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50378..50908)
                     /gene="emrR"
                     /locus_tag="OO439_000359"
     CDS             complement(50378..50908)
                     /gene="emrR"
                     /locus_tag="OO439_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6314826.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(50999..51334)
                     /gene="ygaH"
                     /locus_tag="OO439_000360"
     CDS             complement(50999..51334)
                     /gene="ygaH"
                     /locus_tag="OO439_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6314827.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51324..52061)
                     /gene="ygaZ"
                     /locus_tag="OO439_000361"
     CDS             complement(51324..52061)
                     /gene="ygaZ"
                     /locus_tag="OO439_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6314828.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52185..53369)
                     /locus_tag="OO439_000362"
     CDS             complement(52185..53369)
                     /locus_tag="OO439_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6314829.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53660..54652)
                     /gene="proX"
                     /locus_tag="OO439_000363"
     CDS             complement(53660..54652)
                     /gene="proX"
                     /locus_tag="OO439_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6314830.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54710..55774)
                     /gene="proW"
                     /locus_tag="OO439_000364"
     CDS             complement(54710..55774)
                     /gene="proW"
                     /locus_tag="OO439_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6314831.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55767..56969)
                     /gene="proV"
                     /locus_tag="OO439_000365"
     CDS             complement(55767..56969)
                     /gene="proV"
                     /locus_tag="OO439_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6314832.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57325..58284)
                     /gene="nrdF"
                     /locus_tag="OO439_000366"
     CDS             complement(57325..58284)
                     /gene="nrdF"
                     /locus_tag="OO439_000366"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6314833.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58294..60438)
                     /gene="nrdE"
                     /locus_tag="OO439_000367"
     CDS             complement(58294..60438)
                     /gene="nrdE"
                     /locus_tag="OO439_000367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6314834.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60411..60821)
                     /gene="nrdI"
                     /locus_tag="OO439_000368"
                     /pseudo
     CDS             complement(60411..60821)
                     /gene="nrdI"
                     /locus_tag="OO439_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60818..61063)
                     /gene="nrdH"
                     /locus_tag="OO439_000369"
     CDS             complement(60818..61063)
                     /gene="nrdH"
                     /locus_tag="OO439_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6314835.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61311..61640)
                     /locus_tag="OO439_000370"
     CDS             complement(61311..61640)
                     /locus_tag="OO439_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6314836.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61792..62136
                     /locus_tag="OO439_000371"
     CDS             61792..62136
                     /locus_tag="OO439_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6314837.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62173..62622)
                     /gene="alaE"
                     /locus_tag="OO439_000372"
     CDS             complement(62173..62622)
                     /gene="alaE"
                     /locus_tag="OO439_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6314838.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14823 a        16243 c        16973 g        14949 t
ORIGIN      
        1 cgatggcata ttttatgttg tcattaatga tttcaataat atgtggaagg tgatggaatt
       61 tcagcaacag atctattgcc tcttgttgct ctgaatcatt taaatcgaaa aaccagtcac
      121 caccgctggc aagccacata tttccatcgc cagtttcgaa tgcatccaac gccatcgctt
      181 cgtggttgcc tttaaccgac gtaaaccagg gttggtttag caggcgcagc acgttaagac
      241 tcttcggccc acgatcaata ttatcgcctg tggaaataag taagtcggtt tcagggtaaa
      301 aagagagttg atgttaacgg gattgtaata actgatattc accatgaata tcaccaacga
      361 cccatatatg gcgatagtga tgggcattga ttttttgata gcgtgtagat ggcatggttt
      421 taccctgtaa aataagcttt cctattatac agggtatttt tatttgattc gtcagttgtc
      481 gttaatattc ccgatagcaa aagactatcg ggaattgtta ttacaccaga ctcttcaagc
      541 gataaatcca ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga ttttccagtg
      601 cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa agacatctgc gtaccatcca
      661 cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt tgccgtgcgc
      721 gcttaataac ctctttcggt acgcccgcca gagcagcaac cgccagaccg tagcttttgc
      781 tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc tccagcgcat
      841 cgagatgtac gttggcgacg ccttccattt tctccggtag ctgggtcagc tcgaaatagt
      901 gggtggcaaa cagcgtcaat gccttaatct tattcgccag attttctgcg cacgcccacg
      961 ccagcgacag accatcgtag gtggacgttc cacgcccgat ctcatccatt aacaccagac
     1021 tgtattcggt ggcgttatgt aaaatattgg cggtttcagt catctccacc ataaaggttg
     1081 agcgcccgga agccaggtca tctgccgcgc ctacgcgggt aaagatgcga tcgataggtc
     1141 caatctcgac tttttgtgcc ggtacatagc tgccgatgta ggccatcagc gcaatcagtg
     1201 cggtctggcg catataggta cttttaccgc ccatgttcgg accggtgatg atcaacatac
     1261 ggcgctgcgg cgacagattc agcgggttag cgataaatgg ctcattcagt acttgttcaa
     1321 ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag gtcgggcagg
     1381 tgtagttcag ggtataggcc cgttccgcca ggttcaccag cacgtcgagt tccgccagcg
     1441 cgctcgcgct ctgttgcaac gcttccagat gcggcaacag caggtcgaac aactcttcat
     1501 aaagctgttt ttccagtgcc agtgctttgc cttttgaggt gagaacttta tcttcgtact
     1561 cttttagctc tggaatgatg tagcgctcgg cgtttttcag cgtctggcga cgcatgtagt
     1621 tgatgggtgc cagatggctt tgcccacggc tgatttgaat gtagtagccg tgcaccgcat
     1681 taaagccaac tttcagcgtg tccaggccgg tacgttcacg ctcgcggact tccagacgct
     1741 ccagataatc ggtcgcgccg tcagccagcg cgcgccactc atccagctct tcgttatagc
     1801 ccgatgcgat aacaccaccg tcgcgtacca gcaccggcgg tgtgtcgatg attgctcgct
     1861 ccagcagatc gcgcagctcg gcaaactcgc ccatcttctc acgtagcgcc tgtaccggtg
     1921 cactatcgac agtttctaac tgcgcacgca gctccggcag ttgctggaaa gcgtggcgca
     1981 tacgggccag atcgcgtggg cgagcagttc gtaaagccag acgtgccaga atacgttcca
     2041 ggtcgccgac ctgacgcagt accggctgca actcggcggt gaaatcctgc aatgcgccaa
     2101 tagtttgctg gcgctcaagc aacacgcggg tatcgcgcac tggcatatgc agccagcgtt
     2161 tcagcatacg actacccatc ggcgtgacgg tgcagtcgag cacagaagcc agcgtatttt
     2221 ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg gcatccataa
     2281 tgatgctgtc ctgctcacgt tccatggtga tagaacgaat atgcggcagg gtcgtgcgtt
     2341 gggtatcttt cgcatactgc aacagacaac cggcagcaca aagtccgcgc ggcgcgttct
     2401 cgacgccaaa accgaccaga tcgcgggtgc caaattgcag attcaactgc tggcgcgcgg
     2461 tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc gcgacggcct tcaatcagcg
     2521 acatctcggc gaaatcttct gcatacagca gttccgccgg attagtgcgt tgcagctctg
     2581 ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag ctgatatcca
     2641 gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc cgccagcagg ttgtcctgac
     2701 gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt aacgatacgc acaactttgc
     2761 gctcaaccgg acctttgctg gtcgccggat cgccaatttg ttcgcagatg gcaacggact
     2821 ctccctgatt caccagtttg gcgagatagt tttccaccgc atggtaggga atccccgcca
     2881 tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc agcagttgcg
     2941 acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc acccatccgg taaaacagca
     3001 ggatctcggg atgctgggct ttcagcctga gatactgctg catcatgggc gtatgggcgt
     3061 cgaaattttc tattgcactc atggggttat gtccggttcc ctgatattaa atggggtgtg
     3121 atggttttta ttttcgttgt aatcacataa ggcgcacatg ataccggagt taatcatttc
     3181 aggggaagaa gcgaacggcg agttcttgat gctggttccg ggaaagatgc tgttttgcag
     3241 cttttgcgtg atgcttatga agcaagtacg caaaattgcg tcggttgaat aaacctcctc
     3301 cttcagagcc atagtcccct ttcaaattaa gttgtaatat aaatacacct taagagtatg
     3361 gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg
     3421 atctttatca agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac gaggcgttgt
     3481 ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta
     3541 agcagtgtcc ggtgcactgt taccagcctg caaagcgtga agagatgaaa cagattatgc
     3601 gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg
     3661 atgacaagcg tccggtacca gaactgccgg aaaaatatcg tccgaaaaaa cctcgtgaat
     3721 agtatgccgg acaggcagat ctgtccggca atttgcagtt aaatcaatgc cgatttatca
     3781 attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag acgttgcgca
     3841 gcgcctttag gcccggcaac cacgccgttg gtttctttca gcacgcgcac aatcaactga
     3901 tattcatctt cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc aggttccggt
     3961 aaaacaatat ctggcaatga cagctgcagc acgttaccgc gtgttagcaa taccgcgcgc
     4021 tcaatgacgt tttccagttc gcgtacgtta cccggccact ccatgttgct caaggtgcgc
     4081 agcgtctcgg caggaatgct gtcgatattg cgccccagac gacgggcaat tttgaaggta
     4141 aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg caggtgaatc
     4201 gggaatacgt tcaggcggta atagagatcg ctacggaact ctcggtcggc gaccattttt
     4261 ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat tttgttgctg
     4321 ccgagacgtt caaactcctg ttcctgcaat acacgcagca acttcggctg taactccagt
     4381 ggcatatcgc ccacttcgtc gaggaacaga gagcttttat ccgccagttc aaaacgacca
     4441 atgcgctggg cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag atcgctttcc
     4501 agcaatccgg caggcatcgc cgcgcagttc attttgacca tgcggcgatt attacgccca
     4561 ctgagattat ggatcgcgcg ggcaatcagc tctttacccg tgccagtttc accgaggatc
     4621 agcacggtac tgtcactttg cgccaccatt tcaacttgtt taagcacgct gtacatggct
     4681 tcgctgcggc caataatctc gccaaattca ctatcaacat tgttgagctg ctcggtcagg
     4741 gcgaggtttt catcaaccag ccgttctttc agacgatgga tttcctgata ggcgagggcg
     4801 ttatcgacag cgattgccac acgttcggca atctggcgca gcaaattcag attggtagtg
     4861 gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt gtcgccagac
     4921 atcagcggta acaggcacaa ggtttgaatc tggttgccct aggtgtcgaa caacatgcgt
     4981 tcataggggg ctaaatcgtc ccgctcgtgg agattgatca gcagcatctc tttacttttg
     5041 aacacgcgtt cggtgagggt tccggcttca tcgacttcgc tctgttcgtg ggcgggatgt
     5101 tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt ttttacggtg gctgcgtaag
     5161 acgatactga tatcgtcaat gtcgaaatag taatggattt ctttggcgac ttcgctgacc
     5221 agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag gatgcggaag
     5281 ttatcgcgct cccggcataa caactcatag tcgacattgt tgacgacgcg gctctggatt
     5341 tgttcggtga cgacagaaac gatctgcgta aatgtttgca gacgattgaa ctctttttcg
     5401 ctccagggac gatcgtcata acgaataaat tcacagccac caaaaatatg cccttccgcc
     5461 gccagtggca tcaggcaata gtgaccaaat ttggggtata gcccacctgc gaccagctgc
     5521 ggccaggttt cacaaaattc ttcgtaactg caatgcagcg tatcagggcg cgacaaaatg
     5581 cggcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg gtgtcttttt
     5641 cacgcgatgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca atagccgcgt
     5701 tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc gaggccagat
     5761 cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg agatcactca
     5821 tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc aaaaggtcat
     5881 tgactaacta taggagattt agaattttat tagcatatac gcggaagagg ttcggcgtgt
     5941 ggtaaatcga gggttcgttt cacaccatac agaccggcaa gacgaacacc tttacgttcc
     6001 accacttcac caattagcgc cgcgtctttc cccagtggat gggaatgtaa cgctgccagc
     6061 acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt tgccttcgtt ggcaaagtta
     6121 agggcgtcca gtcccagcag ttcgcaaacg ccgcgcacgg caggtttaac cggcagtgcc
     6181 acttctgaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac taccgcgttc
     6241 acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg cagcgtctga
     6301 ataagcggcg tcagtaccgc gcagtcgctg accagttcgc catccagccc cagctgctca
     6361 cgcaggttaa ggatagtcgc cccgtggtcc ccgagcgtgc cgctaaccag taatacatcg
     6421 cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc catgccagcg
     6481 gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc gccagtaacg
     6541 atggcaatgc ctgccgcgcg cgcggtttct gccatgctgg tcactacggc tttcagtgtc
     6601 tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg aatagcgcca
     6661 ctgaccgcaa cgtcattggc tgtgccgcaa atcgccagct tgccgatatt accgccagga
     6721 aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc gccttctgct
     6781 accagctgcg ccagatcaag acgtgcctgg tcttcctgct ctgccagcca cgggttggca
     6841 aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc gctgccgtgg
     6901 gcgagttgga tattattcac gcttcattct cctgctgacg atactgatac cacgcggcgc
     6961 acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta caggtgttac
     7021 caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca caacgcgcgc
     7081 gcgggtcatc gcagacctgc tgcggtgctg ggcggaaatg tgcttcggca tcgaatcgtt
     7141 gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg cgccattcgc
     7201 tgtcgccgtt gacacagaac acatcagcaa ttgcctgttg cgccagcagg ttaccggcat
     7261 ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt ttctgctcca
     7321 ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc accagcggac
     7381 gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata ctaacgtggc
     7441 ccggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg cgcagcgtcg
     7501 ggataagcgt aatatgctgg cagaagaagt aaaaattctg cacatcgcgg gctttcgcct
     7561 gttgcagggt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg aagaatacga
     7621 ctttacgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag taaacgatgc
     7681 gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttcccc ggcacgcgca
     7741 tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc acgcaggtgt
     7801 cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata aactcaacgt
     7861 tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga ccaccgcaca
     7921 cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca cgttcgcgca
     7981 gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca aaacgcatta
     8041 tttttcctcg ccatacaaca gcgcgccgac atccggctca acgtcaaaca tgttttgtaa
     8101 ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa agccaacgtg
     8161 taccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc cgactaacgt
     8221 taaatcgaca tcgcgctgaa tgccgcagac gtcgactttt gcctggttgc cgtcaatggt
     8281 gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt cagccactgg
     8341 tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg attgacttcg
     8401 cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag gtcaactttg
     8461 ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg tttgtcttca
     8521 ccttcggtaa cggaaagcac cgccactttg tgtttttcac cgagatcgaa gctggccggg
     8581 catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag tggcagacgc
     8641 ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt gttcacctga
     8701 atcgctggtg tgccggtagc gcgaatgcgt gcggcatcgt tcacggtttg ctggtcacct
     8761 tcaataaccg cgcacggaac gctgtctttc aggcgcatta gggtttccgt cagcagggtg
     8821 gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg ggcagcaaag
     8881 cgcgcgcggt tgcgttcagc cagacggtta tttttgtcca gcacgtcaat ttcgacttcc
     8941 agcatccgac gctggctcat gcccggcgcg tgagtgcccg cttcgccatg accgtaatgc
     9001 aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc ggtgattttc
     9061 atcttcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg agggttatgt
     9121 tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca catcgctcac
     9181 tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat ctgtagcatg
     9241 tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac atactgttgg
     9301 catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca accttccgcc
     9361 acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc gacacaagaa
     9421 aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt tgcggcctgc
     9481 tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc gcctcccatt
     9541 aactattgcc agctacaagc aataattgtg ccagtgttga ttatccctgc ggtgaataat
     9601 gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta gcttaaaaat
     9661 ctatttaata acaataaatt aaaagttggc atagaaaatg cttaaagctg gcatctctgt
     9721 taaacgggta acctgacaat gactatttgg gaaataagcg agaaagccga ttacatcgca
     9781 cagcggcatt gtcgcctaca ggaccagtgg cacatctact gcaattcgct ggttcagggg
     9841 atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga caaagaactc
     9901 tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc
     9961 cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag
    10021 gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga tcgcgaacag
    10081 gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata caccgctatt
    10141 gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc ggcagcatga
    10201 gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag
    10261 tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg
    10321 cctggtgaat cgttttgtaa ttgttgactc cacgctctgt atcggctgcc acacttgtga
    10381 ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc gcctgagagt
    10441 gatgctgaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag atgcaccctg
    10501 tgcggtggtt tgcccggtta acgccatcac ccgcattgat ggggccgtgc agttgaatga
    10561 aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg caattgaatt
    10621 ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag cgccaccggc
    10681 accgcctgct ccggcgcgta tcagtacgct gcttgactgg gtgccgggta ttcgcgcgat
    10741 cgccgtcaaa tgtgaccttt gcagttttga tgaacaaggt ccggcctgcg tgcggatgtg
    10801 cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca gcaaacgtaa
    10861 gcgtgagctg acctttaaca cggactttgg cgatctcacc ttgtttcagc aggctcaaag
    10921 tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc
    10981 gccgctgttc tggcatttct cttttctttt caaaaagcgt taagtggctg gatagctgga
    11041 attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt actgactggc
    11101 gcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat ctctccgctt
    11161 aacgcgattt ggctgattac gctcggtctg tgcggtctgt ttgtcagcct ctacaacatt
    11221 gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat gttgatggct
    11281 gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc
    11341 atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact gtggtttgcg
    11401 ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg gcagcgttat
    11461 ggcacgctgg atctgtgcct actggatatg cgtatgcaac agctgccgct cggttccgat
    11521 atctggctgc tcggtgtgat tggctttggc ctgctggccg ggattattcc gctgcacggc
    11581 tgggtgccgc aggcacatgc taacgcctct gcgccagctg ccgcgttgtt ttctacggta
    11641 gtcatgaaaa ttggcctgct gggcatgtta accctgtcac tgctgggcgg taatgcaccg
    11701 ctgtggtggg ggatcgcgct gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg
    11761 tatgcgttga tggaacacaa catccagcgc ctgctggctt accacaccct ggaaaatatc
    11821 ggcattatcc tgctggggct gggcgctggc gtaacgggta tcgcgctcga acaaccggcg
    11881 ctgattgctc ttggcctggt cggtggtctg taccatctgc ttaaccatag cctgttcaaa
    11941 agcgtactgt tcctcggggc ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa
    12001 aaactcggtg gtattggcaa gaaaatgccg gttatctcca tcgccatgtt agtcgggttg
    12061 atggcaatgg ctgcgctgcc gccactgaac gggtttgccg gggaatgggt tatctatcaa
    12121 tccttcttca aactgagcaa tagcggcgcg tttgttgccc gtctgctggg gccgctgctc
    12181 gctgtgggtc tggcaattac cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc
    12241 gtcacgttcc tcggcgcgcc gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc
    12301 ctgatgagcg taagcgtagt ggcactggcg atttgctgcg taattggcgg tgttgctgcg
    12361 ccgtggctgc tgccgatgct ctctgctgct gtacctctgc ctctggagcc tgctaacacc
    12421 accgtttctc aaccaatgat cacattgctg ctgattgcct gcccgctgct gccattcatc
    12481 attatggcga tttgcaaagg cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg
    12541 tgcggctacg accatgaaaa atcaatggtg attaccgctc acggttttgc catgccggtt
    12601 aaacaggcgt ttgcgccagt gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg
    12661 ccgggctggc agtgcgaagg tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg
    12721 gcggtactgg tggtgattat tgtttcacga ggagcctgag aatgagtgtt ttatatccgt
    12781 taattcaggc gctggtgtta tttgccgttg cgccgctgct ctccggtatt acccgcgtgg
    12841 cgcgcgcccg tctgcataac cgtcgtgggc cgggtgtgtt gcaggagtat cgtgacattt
    12901 tcaaactgct gggccgccag agcgttggcc cggatgcctc cggctgggtg ttccgcctga
    12961 cgccgtatgt gatggtgggc gtcatgctga ctatcgctac tgcgctgccg gtggtgaccg
    13021 tcggttctcc gctgccgcaa ctgggtgatt taatcacctt actgtatctc tttgccatcg
    13081 cgcgtttctt ctttgccatt tctggtctgg ataccggtag cccgtttacc gctatcggcg
    13141 cgagccgtga agcgatgctt ggcgtgctgg tcgaaccgat gctgctgctt ggtctgtggg
    13201 ttgccgcaca ggttgccggt tccaccaaca tcagcaacat caccgacacc gtttatcact
    13261 ggccgctgag ccagagcatc ccgctggtac tggcgctttg tgcctgtgcg ttcgccacct
    13321 ttatcgaaat gggcaaactg ccgttcgacc tggcggaagc cgagcaggag ctgcaggaag
    13381 gcccgctctc tgaatacagc ggcagcggct ttggcgtcat gaaatggggt atcagcctga
    13441 aacagctggt ggtgctgcag atgttcgtcg gcgtatttat cccgtgggga cagatggaaa
    13501 ccttcaccgc cggtgggctg ctgctggcgc tggtgattgc catcgtcaaa ctggtcgtcg
    13561 gcgtcctggt tatcgcgctg ttcgaaaaca gcatggcccg tctgcgtctt gatattactc
    13621 cgcgcattac ctggactggg tttggctttg catttttagc gttcgtctcc ttgctggcgg
    13681 cgtgattaaa gagagtttga gcatgtctga agaaaaatta ggtcaacatt atctcgccgc
    13741 gctgaatgag gcatttccgg gcgtcgtgct ggaccacgcc tggcagacca aagatcagct
    13801 gactgtcacc gtaaaggtga actatctgcc ggaagtggtg gagtttctct actacaagca
    13861 ggggggctgg ctgtcggtgc tttttggtaa cgacgaacgc aaactgaatg gtcattacgc
    13921 cgtttattac gtgctgtcga tggagaaggg gactaagtgc tgggtaacgg ttcgcgtcga
    13981 agttgatgcc aacaaaccgg agtatccgtc cgtgacgccg cgcgttccgg cggcggtgtg
    14041 gggcgagcgc gaagtgcgcg atatgtacgg tttgattccg gttggtctgc cggatgaacg
    14101 ccgtctggtg ctgccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca
    14161 gcgtccggca ccgaccaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa
    14221 gaaaaacaac gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggccactt
    14281 ccgtctgttc gtcgatggcg aaaacattat cgacgccgac taccgtctgt tctacgttca
    14341 tcgcggcatg gaaaaactgg cggaaacccg tatgggttac aacgaagtga ccttcctctc
    14401 tgaccgtgtg tgcgggatct gcggctttgc ccacagcacc gcctacacca cgtcggtaga
    14461 aaacgcgatg ggtattcagg tgccagaacg tgcgcagatg atccgcgcca ttctgctgga
    14521 ggtggaacgc ctgcactcgc atctgctcaa cctcggtctc gcctgccact ttaccggctt
    14581 tgactccggc tttatgcagt tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat
    14641 cctcaccggt gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct
    14701 gttgaaagat gacatgatcc agacccgcca gctggcgcaa cagatgcgtc gtgaagtgca
    14761 ggagctggtg gatgtgctgc tgagtacgcc gaacatggaa cagcgcaccg tcggcattgg
    14821 tcgtctggac ccggaaatgg cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag
    14881 cggtcacgcc cgcgatactc gcgccgatca cccgtttgtc ggttatggcc tgctgccaat
    14941 ggaagtccac agcgagcagg gctgcgacgt tatttcccgt ctgaaagtgc gtatcaacga
    15001 agtttatacc gcgctgaaca tgatcgacta cggtctggat aacctgccgg gcggtccgct
    15061 gatggtggaa ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc
    15121 gcgcggcgac gatatccact ggagcatgac cggcgacaac cagaagctgt accgctggcg
    15181 ctgccgtgcc gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gcggtaacac
    15241 cgtttccgat gcgccgctga ttatcggtag cttggaccct tgctactcct gtaccgaccg
    15301 catgaccgtg gtcgatgtgc gtaagaaaaa gagcaaagtg gtgccgtaca aagaactcga
    15361 gcgttacagc attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt
    15421 tatcaaaaaa gtcatcaaaa ccggcacggc gacctcgtct tatccgctgg agccgattgc
    15481 ggttgataaa aacttccgtg gtaagccgga gcagaacccg cagcagtgca tcggctgtgc
    15541 ggcctgcgtc aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccaccgg
    15601 tgagctggcg tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt
    15661 ttgcccgacg gcggcgatca aactgtcgca agagtacgaa ctggcagtgt ggaagaaaga
    15721 agacttcctg caacagtccc gcttcgcgct gtgcaactgc cgcgtctgca atcgtccttt
    15781 cgccgtccag aaagagatcg actacgccat tgcgctgctt aagcacaacg gcgacagccg
    15841 cgcggaaaac caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct
    15901 ggtgccgtcc gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat
    15961 ttattaggcc cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc
    16021 gccagcatga aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctacgt ttatcgcgtg
    16081 gactgcggcg gctgcaacgg ctgcgaaatc gaaattttcg ccacgctttc gccgctgttt
    16141 gatgcagaac gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg
    16201 tttaccggcg cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg
    16261 ccggacccga aaatttgtat ctcctacggt gcctgcggta acagcggtgg tattttccac
    16321 gatctctact gcgtgtgggg cggtacggat aaaatcgtcc cggtagatgt ctacattcct
    16381 ggttgcccgc caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg
    16441 gagcagaaaa ttcacgcccg tggcccgggt gagcaggatg aacaacaggc ggaaatcctg
    16501 catggcgata tggtgcagcc gctgcgtgtg aaagtggatc gcgaagcacg ccgcctggcg
    16561 ggttatcgtt acggtcgtca gattgccgat gacttcctga cacagttagg gcagggcgaa
    16621 gaacaggttg cacgctggct ggaagcggaa aacgatccgc gtctgaacga gattgtcagc
    16681 catctgaatc atgttgttga agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc
    16741 aactgagccg taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg
    16801 tctattacag cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc
    16861 tcacctgccc gtgggatgaa tatctggcat ggattgccac tctggaggcg ggcagtgaag
    16921 cccgccgcaa aatggaaggc gtgccgaaat atggtgagat cgtcatcgac attaaccatg
    16981 tgccgatgct ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagaagg
    17041 aatggagtac aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt
    17101 tgatggtgag gagactgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc
    17161 gatgatggcg ctggcccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg
    17221 gtggtgattg atggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc
    17281 cctacgcggt tactgattgt tgacgctact gatatggggc taaaccccgg cgaaatccgc
    17341 atcatcgacc cggatgatat cgccgagatg tttatgatga ctacccataa catgccgctg
    17401 aactatctta tcgaccagtt gaaagaagac attggcgaag tgattttcct cggcatccag
    17461 ccggatatcg tcggctttta ctacccgatg actcagccga ttaaagatgc ggtagaaacc
    17521 gtttatcaac gactggaagg ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa
    17581 gaagaataat tctccatcag ggaattcagc gaaatgaata tcagaacagg gatgtgcgct
    17641 tttttattga gcttagtgct gcctgcacag gccaccagct ttaccgaata tctgccaatg
    17701 agtgacagcg aatacgcgca aaagcgggcg ctgaaacctc tcctgaccat gccttatgat
    17761 gccgagcaaa cctggcgttt tagaaaagtg ggcgttgctg gcgtgacgct tgaaaagatg
    17821 ccaaatgatg atagtgaatg gcaattgaac ggtaaagatc gcgccggtaa atcctggtct
    17881 gtgcctgtgg gtatgctggc aaacatagcg ggcaaagggc aactctatcg cgccgatctt
    17941 gatcgtaacg gcattcagga tctggttatc tggttaccaa gtaccggtaa tggtctggct
    18001 ccttatgcac atttaattct tatgacgttc acgcgcgaag gccgtccctg tgtctttgaa
    18061 cccaggggat tctacaccgc aagcaaaact ggcgtagatg atttgctgga tttgcaaggc
    18121 aatggtcaca cgcaattgct ggatatgcag tttgattccg gttactggat aaccagtctg
    18181 tatcaggtaa aagacgccaa atggcagcgt gttcacggct ggtttggcaa gttaagctat
    18241 cccgcgctca cgcgttttac ttataccccc aaccgtaaac tcgtcctcaa acccatcgcc
    18301 gggcgcgacc cgcaaacgga agatctggcg cagacgcagc gttgcttgat aaagggggat
    18361 gttcttgatg gggtgaacca gaactgactt tttgcagagt ggctatgagg attttggggg
    18421 taattatatc gcgctcaagt gtttacatat tgtgaacttg gatttgatga gctatagttt
    18481 ccgaacacat aaggatagaa ctcgcagatg tggctgttta cttactataa ggaagttaaa
    18541 acttaaaaat gcacatcatt tctaaagcgc catttgaaga gtctgcgcgt aaatatccta
    18601 acgacgctct ggctcttcag gcattgtatc gggttattaa ggaaacggat ttttctacgc
    18661 cagaagagat gcgaacggcg tttcccaacc tcgacaattt taaatatcgc aacaaatggt
    18721 acgttctgga tgtaggtggg aacaacctaa gggtgattgc ctacataaac tttgttaata
    18781 agcggttctt tgtgaagcac ataactaatc acgccgagta tgacaaactg acacgttact
    18841 acagagagaa caaagaatga ctgctaatgc tgcacgcgct gttaaagcaa caagagaact
    18901 ggtcaatgca gtcccctttc tgggaggtag tgactcagaa gatgattatc gtgaagcact
    18961 ggaactggtc gaatatttga ttgaagaaga tgacaccaat ccacttatcg actttctggc
    19021 gagccgcatt gcagaatatg aaaacaacaa ccaagagttt gcagagttcg acaaagccgt
    19081 tgcggcaatg cctgttggcg tggcgttact tcgcacgctg attgatcaac ataatctgac
    19141 ctatgcggat ttgaagaacg aaatcggctc taaatccctg gtcagtcaga ttttgtctgg
    19201 acaacgatcc ctgacgatat cccacattaa ggcactttca gcccgatttg gcgttaagcc
    19261 ggaatggttt ctttgatttt gactgatgcc aaaccccaga ttgcccgatg cgctgcgctt
    19321 atcgggcctt cctggttcgt gcgacatgta ggccggataa ggcgttcacg ccgcatccgg
    19381 cactgttacc tactctaaat cttccccatt actggcaatc acttttttat accaccagaa
    19441 cgatttctta cgtgtgcgcg tcagcgtgcc gttgcctgcg tcgtcacggt ctacgtagac
    19501 aaaaccgtag cgtttgctca tttcacccgt agaggcggag actaaatcaa tacagcccca
    19561 ggtggtgtag cccatcagcg gaatgccatc tgcaatcgct tcgcccattg cgcggatatg
    19621 ttcgcgtaaa tagctgatgc gatagtcatc gttaatctcg ccattggcag caagttcatc
    19681 ttttgcgccg aggccgtttt ccaccagaaa cagcggcttc tgataacggt cgtacatcat
    19741 gttcatggtg atacgcagac cgagtggatc aataccccag ccccagtcgc tcacttgcag
    19801 atacgggtta cgcagcgatt tcactacgtt cgccgcactg ctattgttgg cgttcatctc
    19861 cgccgaggcg cagcgcgagg cgtaatagct gaaagagaca aaatcgacgg tgtttttcag
    19921 gatttcatca tcgcccggtg ctttgtcgat ggttacccct ttttcgcgga atacgcgggc
    19981 agagtaagcc ggatacgtgc cccgcgcctg cacatcgata aaaaacaggt tttcccggtc
    20041 tttctccagc gccgcccaga catcttccgg cttgcaactg taagggtaga agttaccgcc
    20101 cgccagcata cagcccacct gattttgcgg gttaacctca tgggcgattt tggtggctag
    20161 cgcactggca accagctggt gatgcgcggc ctgatatttc acctgatcct gattttcacc
    20221 ttcttcaaac accagacccg cgccggagaa cgggctatgc aacataatgt tgatttcatt
    20281 gaaggtaagc cagtatttca ccagaccatc aaatgcttca aagcaggtcc gggcgtagcg
    20341 gctgaaaaac tccaccagct tgcggttacg ccaggagcca tattcggtga ccagatgcat
    20401 cggcacatcg aagtggcaca acgtgaccag cggttcgata ccgtactttt tacactcttc
    20461 aaagacagaa cgataaaaag caatgccctg ctgattgggc gtgatttcat cgccctgcgg
    20521 aaagagacgg ctccaggcaa ttgaggtacg gaaaaccttg aatcccatct ctgccatcag
    20581 ggcgatatct tctttataac gatgataaaa atccgtcgcc tcatggctgg gataaaactc
    20641 gtcatcgcgc aactgaaaac gtttttccag ccccagtttc accgccattc gatgctcgcc
    20701 gtgtgggatc atatcgacag tggtcagccc tttgccacct tcacggaacg caccttcaga
    20761 ctggttggcg gcaagcgcgc cgccccataa aaaaccttct ggaaatactg acattttcat
    20821 cctcaattaa gacttacttc tttggcgacg gtcggttgta cgctctgatg ctttcgcgcc
    20881 tcggcagccg cttcctcaac aggaatatcc tcaaagccga gcaacagtgt gaggataaac
    20941 gacagcacca ccgccagcgc catgaccgcg aacacccaga cgatgctcat tggattcgcc
    21001 ggatcgaaga attgcacgct ggtaaataac cctggcgctg ccattgagtg gctggcaagc
    21061 cccgccatac cggcaaccgc gccgcaaata aaaccgctga taagactggc gataagcgga
    21121 cgtttcaggc ggatcgccac gccgtataac gccggttcgg aaatccccgc cataatggct
    21181 gatgccgccg cagccagcgc cgtctggcgc agttccgggt ttttcgtttt ccacgccacc
    21241 gccagtgatg aaccgcccag cgacaggtta gcgccgatct ctgacggcat gaccatccct
    21301 tctttgccgg tttcggcaat ggtctgaatg atggttggcg taaagacgcg gtgcatcccg
    21361 gtcattacca gcagaggcca cagcgcgccc ataatggcga ctgaaagcca gcccagataa
    21421 ccatgaatgg tgtaaaccag cgccgaaatg gcgctaccga tccagatacc aatcgggcca
    21481 atcagcagga ttgccagcgg tgcggcaatc aacacaatca gcattggctt gaggaagttt
    21541 ttggtcaccg ccggtgtaat gcgatccacc cagcgttcga tatatgacag gcaccaggtc
    21601 atcaccagcg ccgggatcac tgtgtaggtg tatttcaccg cggtgaccgg aatcagggca
    21661 aattcgacat gttcaccctg ggccgctttc gccatcagtt caataaaact cggatgtacc
    21721 agcacacccg cgatggcaat cgccagcgac atattggttt taaatttgat ggcggcagat
    21781 gccgcgacca tcagcggcag gaagaagaaa gcaccgtcac caatcacatt cagaatggtt
    21841 aaggtcggcg atccttttgt cagcacgccg ctcatctcga ggatcatcgc cagcagtttg
    21901 accatcgatc cgccgataat cgccgggatc agcggtgaca tggtgccgat cagcgcatcg
    21961 aggatccccg caccaatgcg acgcagcgtg agtttgggtt tacccacggg ctgtgcgggc
    22021 tgcatatctc ccggcagcag gctgacgact tcctgaaagg cttgtgaaac ggtattgccg
    22081 ataatcacct gacactggtt gtcactacgt accacgccga gcacgccggg gatggttttt
    22141 aacgtcgggc tgtcgataag ctggtcatct ttgataacaa cgcgcaaacg cgtcatacag
    22201 tgcgtgaccg ccgagatgtt atcaacgccg cccagtgccg ctatcaccga gcgtgccagc
    22261 gccgcataat ttttggccat cggattttat cctgttatca gtagggtaga tgtagttgtt
    22321 atcagtcgac gtaacggaaa aatgccgatg ctgaaacgga attaggaaac cggtttcaca
    22381 aaatcatgaa tagttgtcac taaataagca agagagttaa ttgttcattt tttatatttg
    22441 tgaaaccggt cacctgaata tttttcccgc cgattgcgca aggacaatcg gcaaacggtg
    22501 cagtacactg cgttgattgc gtgtaaaggg atgaaaatga tgacgacgat gctggaagtg
    22561 gcgaagcgcg ccggggtttc aaaagcgacc gtttcccgcg tgctttcagg taatggctac
    22621 gtcagccagg agactaaaga tcgcgtgttt caggcggtag aagagagcgg ttaccgccca
    22681 aacttgctgg cgcgcaatct gtcggcgaag agtactcaga cgctggggtt ggtagtgacc
    22741 aacacgcttt accacggcat ttattttagt gaattactct ttcatgccgc gcgaatggcg
    22801 gaagagaaag ggcggcagtt gctattggca gatggtaaac acagcgccga agaagagcgc
    22861 caggcgattc agtatctgct ggatttgcgc tgcgacgcca ttatgattta cccgcgtttt
    22921 ttaagcgtgg atgagatcga tgacatcatt gacgcgcaca gtcagccgat aatggtgctt
    22981 aatcgccgcc tgcgcaaaaa cagcagccat agcgtctggt gcgatcataa acagaccagc
    23041 tttaatgccg tggcagagtt gataaacgcc ggacatcagg agattgcttt ccttaccggc
    23101 tcgatggatt cccccaccag cattgaacgt cttgctgggt ataaagacgc gctggcgcag
    23161 catggtattg cgctcaatga aaaacttatc actaacggta aatggacgcc cgccagcggg
    23221 gccgaagggg tagaaacgtt gctcgaacgt ggggctaaat ttagcgcgtt agttgccagt
    23281 aacgacgata tggcgatagg tgcgatgaaa gcgttgcacg agcgcggcgt agcggtgcca
    23341 gagcaggtgt cagttatcgg attcgatgat atcgcaattg ccccctatat cgttccggcg
    23401 ctctccagcg taaaaattcc ggtgactgag atgattcagg aaattattgg acggctgatt
    23461 tttatgctcg atggcgggga tttctcaccg ccgaaaacct tcagcggaaa actgatccgc
    23521 cgcgactcac tcattgctct ttcgcgataa acattgtcat cacatgtcat cgtcactagt
    23581 gtcgatgaca tcttttttcc tgcattacag aaattaattt ccttaaataa cagtgaatta
    23641 aaactggcat gatttgtgaa tgtatcggcg cattaactgt cattgctgga gaatttgatg
    23701 aaccgtttca tcattgctga cgcgagtaaa tgtattggtt gctgtacctg tgaagtggcg
    23761 tgcgtggttt ctcatcagga aaatcaggac tgtgcatcgc tgaccccgga aacattttta
    23821 ccgcgtatcc atgtcattaa aggtgtgaac atttccacgg cgacagtttg ccgtcagtgt
    23881 gaagatgcac cgtgcgctaa cgtctgcccg aatggtgcta tcagccgtga taaagggttt
    23941 gttcatgtga tgcaggaacg ttgcattggt tgcaaaacct gcgttgtggc ttgcccgtat
    24001 ggcgcgatgg aagtggtggt acgtccggtg attcgcaaca gcggcgcggg gctgaatgta
    24061 cgggctgaca aagccgaagc caataaatgt gacctgtgca accatcgtga agacggcccg
    24121 gcgtgtatgg cggcttgccc gactcatgcg ctgatttgtg tcgatcgtaa taaacttgaa
    24181 caactgagtg cagaaaaacg ccgccgtacg gcgctgatgt tctaaaaatc ttacagaact
    24241 gttctggcat caaaggaatt cccggaaata ttcgtagaat acggagctgg cggtatcgcc
    24301 gacctgagac tgccggatac gacgcagtcg tgtccggcag gacgggttac gcaattcatg
    24361 atggcgggaa taactcaatg gcaaaaaaca catcttgcgg tgtccaactg cgtattcgtg
    24421 gcaaagtgca gggcgtcggt tttcgtccgt ttgtctggca gctggcacag caattaaatc
    24481 ttcacggcga tgtctgtaat gacggcgatg gcgtagaagt ccggctgcgg gaagacccgg
    24541 aaacgttact tgttcaattg catcagcact gcccgccgct ggcgcgtatt gatagcgtcg
    24601 agcgtgagcc gtacatctgg tcacaactgc ccactgagtt caccatccgc cagagcgcgg
    24661 gaggtgccat gaatacgcaa attgtcccgg atgccgctac ttgccctgct tgccttgccg
    24721 aaatgaatac cccaggcgaa cggcgttatc gttatccgtt tatcaactgt acccactgcg
    24781 gcccgcgttt caccattatt cgcgccatgc cttacgaccg cccgtttacc gtgatggcgg
    24841 cgtttccgtt gtgtccggct tgtgacaaag agtaccgtga cccgctcgat cgtcgcttcc
    24901 acgcccagcc ggtggcctgc ccggagtgtg gcccacatct tgaatgggta agtcatggtg
    24961 aacatgcaga acaagaggcg gcattacagg cggctatcgc acagttaaaa atgggcaaca
    25021 ttgtcgccat caaagggatt ggcggatttc atcttgcctg cgatgcacgt aacagtaacg
    25081 cggtggcgac acttcgggcg cgcaaacatc gcccggcgaa accgctggcg gtcatgttgc
    25141 cagtggctga cggtttacca gacgctgcgc gccagttgct taccacgccc gccgcgccga
    25201 ttgtgctggt ggataaaaaa tacgttcctg agctttgtga tgatatcgcc cctgacctta
    25261 acgaagtcgg ggtaatgttg cctgcgaacc cgctccagca tttgctgtta caggaactgc
    25321 aatgcccgct ggtgatgacc tccggcaacc tgagcggtaa accaccagct atcagcaacg
    25381 aacaggcgct ggcggatttg cagggcattg ccgacggatt cttgatacat aaccgcgaca
    25441 tcgtgcagcg gatggatgat tcggtggtgc gcgaaagcgg cgaaatgctg cgccgttcgc
    25501 gggggtatgt gccggatgcg ctggctttgc ctccgggctt taaaaatgtt ccgcctgtgc
    25561 tgtgtctcgg cgcggatctg aaaaatacct tctgcctggt gcgcggtgaa caagcggtgt
    25621 tgagtcagca tctgggcgat ttaagtgacg atggcatcca gatgcagtgg cgcgaagcgt
    25681 tacgcctgat gcaaaacatc tacgatttta ccccgcaata cgttgtgcat gacgcgcatc
    25741 cgggctatgt ctccagccag tgggcgcgtg aaatgaatct gccgacgcaa acggtactgc
    25801 atcatcatgc ccatgcagcg gcgtgtctgg cagagcatca gtggccgttg gatggcggtg
    25861 atgtcattgc tttgacgctc gacggtatcg gtatggggga gaacggcgct ttgtggggcg
    25921 gcgagtgcct gcgggtgaac tatcgcgaat gtgagcacct gggcggcttg cctgcagtgg
    25981 cgcttccggg tggcgatttg gcagcgaagc agccgtggcg aaacctgctg gcgcagtgcc
    26041 tgcgctttgt gccggagtgg cagaattacc ctgaaacagc aagtgtgcaa cagcaaaact
    26101 ggagcgtgct ggcgcgggcc attgagcgtg gaattaacgc gccgctggcg tcatcgtgtg
    26161 ggcggttgtt cgatgcagtg gcggcggcac tgggctgtgc gccagccacg ttaagttatg
    26221 aaggtgaagc ggcttgtgct ctggaggcgc tcgcagcctc atgccacgga gtgacgcatc
    26281 cggtgacgat gccgctggtg gacaatcaac tggatctcgc cgctttctgg cagcagtggc
    26341 tgaactggca ggcaccggtt aatcaacgcg cgtgggcgtt tcatgatgcg ctggcgcagg
    26401 gttttgccgc gttgatgcgt gagcaggcca cgatgcgtgg tatcactacg ctggtattta
    26461 gcggcggggt tattcataac cgtttgctgc gtgcacgtct ggcgcattat ctcgctgatt
    26521 tcacattgct ctttccacag agtttaccgg cgggtgatgg cggtttgtct ctggggcagg
    26581 gggttattgc tgcggcgcgt tggttagcgg gtgaagtcca gaacggataa aacgtgttgc
    26641 atttggacac cattgccgga tgcggcgtaa acgccttatc tggcctacac atgcgtcccc
    26701 gtaggtcgga taagacgcgc cagcgtcgca tccgacattt taggcacagt agcccaccta
    26761 catcggcaat gttttcaaca atccaaatgc ctctttcatc cgatcctcac tgaccacaaa
    26821 ggcgcgaagc tggtcagcat cgtcaacacc gcgagccacc attccctggc gttcggtatt
    26881 aatttgccag cgtaaatcct gacgctgggt ttcgcctgcc agatgcagcg gtaattccgg
    26941 cgttttgatt ttcaccagca tcgccggtag tttcagcggc gtgttattgc cgagaagatt
    27001 ttttgccagc accatcgcgc taagttgaat cggctgcagg aatggcagta cctgaccgtt
    27061 gatttccgcg caatcgccca gcgcataaat atcgtcatta ctggtttgca gataactatc
    27121 gacgcaaacg ccgcgattaa tcgtcagccc ggcgcgtcgt gccagggctg tttccgggcg
    27181 cagtccggtg gcggcaatta ccgcatccac ttcgatacta cgctggtggt ccagcgttgc
    27241 cagaatgcca gaatccgttt tttccagccc ctgcaactga gatttcaaca gcagatgaac
    27301 gcccatctcc gtcaaccgat gctgcaagcg gctgcttact tctggtggca ttaatgacgc
    27361 cagaatactg gcagcgttgt cgattagcgt gaccgctttg cctgcacgac aaaaatccat
    27421 cgccagttcg ctaccaatca aaccaccgcc gacaatcaac acgcgtcggg catcccgcag
    27481 ttgcgtttca caggcgcgat actcttgctg actatttaac gtcagcatta actcacgccc
    27541 aggcactggc gggacaaagg cactggcacc agttgccagc accagcttgt cgtattgcca
    27601 ctgattattc tggcttttca ccacatgggc ttcggcatcg atatccgtca cccaggtgtg
    27661 cggaaacagg tgcagattaa actgcccggc aaattcaccc gccgtctggc gggtaaggtc
    27721 gtcggcacgt tgcccctggc tgataacatg gctgaggtcc ggtttgttgt actcatccat
    27781 gctgtcggcg gcgatcaggg ttaatggaat actggcgtcc tgtttgcgaa tatttttcac
    27841 cagttggcgg gcggcgaagc ccgaaccaat gatcacaatg ccgttgctca ttttgcctcc
    27901 gatgccagtt cgtcaaagac gtctttgccg agagagcatt ccgggcagag gaagttatcc
    27961 ggaacttcac tccacggcgt tcctggcgca acgtcctgca ttggctcgcc ttttgccgga
    28021 tcgtaaatcc actggcagac gctgcactgc atccgtgggc cgaggtcgga cttcgtggtg
    28081 gcagaggttt cttctttaac caccgtattc accgtgctct gcggcagcgg cgcgagcgcc
    28141 cactgacggg cgatttcgcg accatgttca cggcataact ccagagcgtc ctggtctggt
    28201 cgccatttgg ctttcaggct aagcgacatt tcgaaacccg catcctgcag gcgcgtggaa
    28261 agacgatcca ccgcaccgcc gctccagccg tgagagccga aagcactggc gcgtttgtta
    28321 cggaagcgta atccggtcat ctcctccacc agaccggcga ttttcggcat catcacgtta
    28381 ttcatcgtcg aagtgccgac cagcacgcct tttgagcgga agacattggt caggatttcg
    28441 tttttatcgc ttcgggcgac gttgaaaatt ttcaccgcca cgcgtgggtc ggtttccgca
    28501 atcccctggg cgatagcgtc agccatcatg cgggtgttat tcgacatggt gtcgtagaaa
    28561 atggtgattc tgtcttcctg ataatcagcc gcccatttca ggtacagctc gacaatttgc
    28621 gtcgggttat cgcgccatac cacgccgtga gaggtcgcta tcatatcgac tggtaagtta
    28681 aagcccagga tctcggtaat tttcggtgtt accaggcggc tgaacggcgt caggatattg
    28741 gcgtagtaac gctggcactg ctcgaaaagc tccgtctgat ccacttcatc gttgaacaga
    28801 tgctcgtcgc agtagtgctg accgaaagca tcgttactga acagcaccgc gtcgcctgtc
    28861 aggtaagtca tcatgctgtc cggccagtgc agcattggcg tttcgacaaa aatgagctgt
    28921 ttgccgttgc cgatatccag cgtgtcacca gttttcacca cattaaaatt ccactccgga
    28981 tgatgatgat gaccatttat cgagtcgata gcgttggcag tacagtaaat cggcgtatcg
    29041 ggaatttgcg ccatcagttc ggtcagcgcc ccagcgtggt cctcttctgc atggttaatc
    29101 acgatgtaat cgatatccgc cagatcgatt tcattacgca ggttctgcac aaattcgcgg
    29161 ctgaatttat ggtcgacggt gtcgatcagc acgttttttt cttcgcggat gaggtagctg
    29221 ttgtaactgc tgccgcgtag cgttttatat tccgtaccgt gaaaatcacg cacttcccag
    29281 tcacgttgac caacccaatg aatgttattt ttcaccacaa tagacatagc aacctcaatt
    29341 tattcagcgt gttctaaaaa gatgtcttgc taattgcaga ttacgtgcca gttttttatc
    29401 tgattgtttt tactctataa ataaaattat gctcattaat gagtagtcaa aatgactatc
    29461 aatcaaatgg tcgatatgac aatatctata gtcaaattga cagtgaggca aagatgagtt
    29521 tttccgttga tgtgctggcg aatatcgcca tcgaattgca gcgtgggatt ggtcatcagg
    29581 atcgttttca gcgcctgatc accacgctac gtcaggtgct ggagtgcgat gcgtctgcgt
    29641 tgctacgtta cgattcgcgg cagtttattc cgcttgccat cgacggtctg gcaaaggatg
    29701 tactcggtag acgctttgcg ctggaagggc atccacggct ggaagcgatt gcccgcgccg
    29761 gggatgtggt gcgctttccc gcagacagcg aattgcccga tccctatgac ggtttgattc
    29821 ctgggcagga gagtctgaag gttcacgcct gcgttggtct gccattgttt gccgggcaaa
    29881 acctgatcgg cgcactgacg ctcgacggga tgcagcccga tcagttcgat gttttcagcg
    29941 acgaagagct acggctgatt gctgcgctgg cggcgggagc gttaagcaat gcgttgctga
    30001 ttgaacaact ggaaagccag aatatgatgc caggcgatgc cacgccgttt gaagcggtga
    30061 aacagacgca gatgattggc ttgtcccctg gcatgacgca actgaaaaaa gagattgaga
    30121 ttgtggcggc gtccgatctc aacgtcctga tcagcggtga gacgggaacc ggtaaggagc
    30181 tggtggcgaa agcgattcat gaagcctcgc cacgggcggt gaatccgctg gtctatctca
    30241 actgtgctgc actgccggaa agtgtggcgg aaagtgagtt gttcgggcat gtgaaaggag
    30301 cgtttactgg cgctatcagt aaccgcagcg ggaagtttga aatggcggat aacggcactc
    30361 tgtttctgga tgagatcggc gagttgtcgt tggcattgca ggccaagctg ctgagggtgt
    30421 tgcagtatgg cgatattcag cgcgttggcg atgaccgtag tttgcgggtc gatgtgcgcg
    30481 tgctggcggc gactaaccgc gacttacgcg aagaggtgat ggcagggcga tttcgcgctg
    30541 acttgtttca tcgcctgagc gtgtttccac tttcggtgcc gccgctgcgt gagcggggcg
    30601 atgatgtcat tctgctggcg gggtatttct gcgagcagtg tcgtttgcgg ctggggctct
    30661 cccgcgtggt attaagtgcc ggagcgcgaa atttactgca acactatcgt tttccgggga
    30721 acgtgcgcga actggaacat gctattcatc gggcggtagt gctggcgaga gccacccgca
    30781 acggcgatga agtgattctt gaggcgcaac attttgcttt tcctgaggtg acgttgccgc
    30841 cgccagaagc ggcggcggtg cccgttgtta agcaaaacct gcgtgaagcg acagaagcgt
    30901 tccagcgtga aactattcgc caggcactgg cacaaaatca tcataactgg gctgcctgcg
    30961 cgcggatgct ggaaaccgac gtcgccaacc tgcatcggct ggcgaaacgt ctgggaatga
    31021 aggattaaat aatcccggcc tgatagaaat cctgcaggtt aattgcgccg gtgagtttgc
    31081 cgttttcatc caccaccggt gcggcagtga ttttgcgctt catcaggatc tctttggcgt
    31141 cgatggcgcg actttgcgcc tgtaacgtgg tgccgcctgt cgtcatcgct tcattaactg
    31201 gcgtggtgag tgcgccgccg cctaccagcc agcgacgtaa atcgccgtcg gtaaagacgc
    31261 cttgtacctg ttgttgagcg tcacataccg ccaccagccc cagaccggtg cggctgagtt
    31321 ccagcatcgc atccatcacg ctggcggtta acgccacctg tgggatggca tcgtcacggc
    31381 gcatcagatg atgcacttta ttcagcaagc gagcgcccag tgccccggct gggtgggagc
    31441 gggcaaaatc ttcttcatta aatccgcgcg cctgcatgac cgccatcgcc agcgcgtcac
    31501 ccatcatcag ggtattgacg gtgctggagg tcggcgcaag gtgcatcggg caggcttcgc
    31561 gttctacgga gatatccagc accgcttttg ccgccaggcc cagcggtgac gtcggtttgc
    31621 cggtcatcgc cagcagcgcg atagatttat cttccagacg cggaataatc agatccagtt
    31681 ccttcgcgcc accggagtaa gagataaaca gcatcacatc gcggctttca atcatcccca
    31741 gatcgccgtg cagtgcttct gccggatgga caaaaaaagc cgatgtgccg gtactggcaa
    31801 gtgttgcggc gattttctta ccaatgtggc ccgatttgcc aattcccgaa accaccactt
    31861 tgccttcgca gtgcaggatg atattggcgg cgcgaacaaa atcatcgccc agacgttccg
    31921 gtaaacggct tgcttcctgc aactccagca ttaacgtctg acgtcccgcg ttcagtagtg
    31981 cttcactcat tgctctctcc ggttatgatc acatcgatcc ctttctcttc cagcgcctga
    32041 cgaaacgccg gatcgatacc tgcgtcggta atcagcttat cgacgctttc aagactgcaa
    32101 actacgttgg ggcttttacg gccaaacttt gatgagtccg ccatcaaaat cacttcgcgc
    32161 gcggcattgc acattgcctt actgacggta taaacctcgt taaaggtggt tacgcccgca
    32221 ttgagatcga tgccgtcggt gcccataaac aatttatcga aactgaaatg ttcgaaggca
    32281 ttctctgcca gctgtccgtg aaatgaggcc gattttttac gaaacgttcc gcctggcatc
    32341 aggatagttt gttcgttatc cagttcagat agcgcattga cgatatgcag actgttggtc
    32401 atcaccgtga tgttattaaa gcgcgagagc aggggaacca tctgcaaaac ggtactgcca
    32461 gcatcaagaa tgatcgaatc gccatcatga ataaaactaa cggcagcttc tgctatcagc
    32521 tctttcttgt gggtgttgat gagtgtttta tgatcgatag gcggatcgga ttcctcttta
    32581 ttcaacacca ctccgccata agtacgaatg acggttcctg catgttccag aatgaccaga
    32641 tctttgcgaa tggttgtgcc tgtggtgtca aagtattgcg ccaattcttc aaccgagcat
    32701 ttaccctgct tttgcagata ctccagaatg gcggcctgac gctgacgagg tttcatgggc
    32761 gtgattacct gatttaggtt tcaaaatgat aacttcgcaa gcaagtaaca ttcacaacga
    32821 aattatccac gtttcagttt aagcgccaat gatagagcat tctgtgatag tggatcatgg
    32881 ggaaagatca catcgggctg taaatcaccc gcatgcatac cggtaattgc gggaattttt
    32941 tgcggtcgtg cgaagacggt cagtcctttc ataaacaagg tgtcgacgat gcgctgctga
    33001 tcgtccaggg cgatggcgac cacgacccgt ggtttaaagc gcccgctgga acggcccacg
    33061 ccaacccgcc cttgctggca tagtgtgtcg aaggcacggt taaaacgaga aatttgccag
    33121 ccgcctaagg ccagttgcgc acaccaggcg ataacggcga cggtgatgag tgcggatacc
    33181 ataacttttc cttaatgtag tggcggaagt cctgtatgcc tgatgagcgt agcgcatcag
    33241 gcattctatt agtctcacgg agatccttaa cggatctccg ctgttgatta caacatcacc
    33301 tgaccgccgg tgacattgat cgactgtccg gtgcagtacg acgccttagg actggcatag
    33361 agcagcagca tattcagcac atcctgataa tcgcagccgc gtttgagcgg tactttgtcg
    33421 atgtaatact gctcgacttg atccggtttg atacccagct tggtcgcgta ttggggtaac
    33481 agtgactgga acataggcga tttcagcagg ttaccgagca tcagtgaatg caccgtaatg
    33541 ccgtactccg ccagatccag cgccagtgat tgagtcagcc cgacgccacc aaatttcgct
    33601 gcgctgtagc cagagttgtg tttgctgccc actttgccgg atttcgagtt gatctgaata
    33661 atgcgaccct gaatcccgtc gcggatcatc aaacgagaaa attcacgggc acacaggaaa
    33721 taacccacca gattcacggt caaaatcgcc gagctggaag tcgctgataa aggctgcttt
    33781 tgctattccg gcgctgtaga ccagcaaatc cacgcgccca aagatttcat ctaccccacg
    33841 agagagcgcc aggacgcttt gctcgctggt ggcgtcagca ccaaaactat acgctgttcc
    33901 ttcaccatat tcggcgttaa tttcttgtgc cacatttgcg gctttgtcgc tctgaatatc
    33961 gacaaccgcg acgcgatacc cctcggcagc cagaccgtgg cacaggaacg cgcctaaggt
    34021 ttgcccacca ccgatgacaa cggcaacctg attcattttt tactccttaa cagattcaaa
    34081 cttcaaaacc gatcccggcg cgatatcgtc gggtacaggg cctgccacat ggacagtgcc
    34141 cggaaattcc gcttcgttta aaccatcgaa gcgcagggtg acatgaccca gttcgcgaag
    34201 gttgtcttcc gccacgctgc caacagcggt caccggatag cgatgctgcc cgagcgtaaa
    34261 ttgcaaaccg ggatggagtg cacctttcaa ctcgccgtgg caatgaatga agcaatactc
    34321 ttcgaggtcc gctggcgcgc cttcacgaaa ggtgatgagc atctggtcgc tgagggcgtc
    34381 agtggcactc gcgccgatac gggtgatggt ggtctgataa ataacggtca tgtttcagcc
    34441 tctattgata gataaaaccg gagacaaacc aggcgatcag tacagtcggt gcgccggtta
    34501 aaaagtggct caccagtaca gaagggacac cgacgcgaac cgtgtcctga cgggcttccg
    34561 ccagcgacaa accgaccggg atgaagtcgc aggccgcctg cgcgttgatg gcaaacagtg
    34621 ccggtaaagc cagatgcggc ggaatattgc cgagaccaat ctgcacgcca atcaatacgc
    34681 cgataacctg tgcgataact gcgcctgggc cgaggaaagg tgaaagcagc gggaaggagc
    34741 agatgagcgc cagcatgacc agacctagtg gatggctcgc cagcggagca agaccgtggg
    34801 caatccagtc accaaggcca gaagccataa tgatgccaat gagcgccgag acgaacgcca
    34861 taaacggcag aatggttttt aatacagtgt cgatggtgtc acgaccagat tgaaacaaca
    34921 cggcaacggc agaacccata cccattccca ccttcgccag taaaccatcg ctttgttcgg
    34981 tgattttctt gctggtgtca tagtcacggc ccacggaaga gggttgtggt gtcgcatcac
    35041 ccactacagt gatgttttct tcttttacgc cagagacata aatatcttcc acaatgtact
    35101 gcgccagcgg actggacttg cccgtcgagt ggatattaat ggtgggaata cgtcgtttcg
    35161 gatagatgcc gcagcgtaat gtgccgccac agtcgattac cgcgacacca atttccgcct
    35221 ccgcgggttc accttcttta aatccgtcaa tagcctgcca gccagtaagt tgtgccagtt
    35281 tgtcaacaat cgcaggccgg gtaccggcgg tgatatagac gatttttttg cccggcgtgg
    35341 cttccagctc aagcgggccg ccccagccac ccgttccttt ttcgatccga atacgcgtca
    35401 tgatgttgct cctgccaggt gaactttttg ttcaagttga atgcccatct ttttctcaaa
    35461 aatggcggtg gtcagatcgg tcacccagcc gcgaaagaag ttggtgacca gaccaaccag
    35521 cagatagctc accgccagtg ggccaagtgg caggttcagc gttgtcagac cgctggcaat
    35581 gccaagataa acaaacagtt cgccagggtt gatatgggga aagaggccat tcattgagtg
    35641 gcagctataa gaggctgccg cgtagtagct gggtttgtac ttttccggca taaagcgacc
    35701 caggcttaag gtcatcggat tgcaaaagac aaacgtgcca atgcacggta gcagcaggta
    35761 acgggaaaca gggttaccgg cgcaacgttg agcaaaacgt tcaatacgat gctgaccgat
    35821 aaaattaatc agtgcgttca tgataaccag caggctaatc aacagcggaa gaatgccggt
    35881 caccatcccg gtaaacacct ctccgccctt ttggaacagc ccgataaacc actctgcacc
    35941 atgagtaatg gtttctatca ttgttctctc cttcaggatt tattgtttta ttaccaaacg
    36001 gcaacctaat ctaatcagat tgaaagattt gaaagtgtta ttttgatcgc aaaatgaaag
    36061 ataaatattt taatttgaaa gtttgaataa aaggatagcg aggggaatga gttgagttat
    36121 gtaaagtccg tatcgggcag tgactaccgc ttccttgtgc ggggcgtgat gctttaccat
    36181 acttgcccct ggttgaatct gttaaatgga cccctcatgt tcaagcgtcg ttatgtaaca
    36241 ttgcttcccc tttttgtgtt gcttgccgcc tgtagcagca agccaaaacc tactgagact
    36301 gaaacgacca ccggaacgcc gtctggcggc ttcctgcttg agcctcagca caatgtgatg
    36361 cagatgggcg gcgatttcgc taataacccg aatgcccagc agttcatcga cagaatggtg
    36421 aacaaacacg gtttcgatcg tcagcagttg caggaaattc tctcccaggc gaagcgtctg
    36481 gattcggtac tgcggctgat ggataaccag gcaccaacca catcggtgaa accgccatca
    36541 ggtccgaacg gcgcatggct gcgttatcgc aaaaaattta ttacgccgga caacgtgcag
    36601 aacggtgtgg ttttctggaa tcagtatgaa gatgcgttga atcgcgcgtg gcaggtgtat
    36661 ggcgtaccgc cggaaattat cgtcgggatt atcggcgttg aaacccgctg ggggcgcgtg
    36721 atggggaaaa ctcgcatcct cgatgcgctg gcaacgctgt catttaacta cccacgccgc
    36781 gcggagtatt tctctggcga gctggaaacc ttcctgttga tggcgcgcga cgagcaggac
    36841 gatccgctca atctgaaagg ttcctttgcc ggggcgatgg gctacggaca gtttatgccg
    36901 tcgtcttaca aacaatatgc ggtagatttc agcggcgacg ggcatatcaa cctgtgggat
    36961 ccggttgatg cgatcggtag tgtggcgaac tatttcaaag cgcacggctg ggtgaaaggc
    37021 gatcaggtcg cggtaatggc aaacggtcag gctccaggct tgccaaatgg cttcaaaacc
    37081 aggtacagca tttcgcagct tgccacggca ggtttaacgc cacagcagcc gctgggcaac
    37141 catcaacaag ccagcctgct gcgtctggat gtcggtaccg gttaccagta ctggtacggt
    37201 ctgccgaact tctacaccat tacccgttat aaccacagca cccattacgc aatggcggtc
    37261 tggcagttag gacaagccgt ggcgctggcg cgcgtgcagt aactatttgc aagagggggg
    37321 cgtgagcttc cctctggcta atcagctttt ttctgaatcc tcctcgtaaa attgcaacgc
    37381 caacaccatc ttcctgacga aagtgctatc ttgtccggca taaatttttg actgactgag
    37441 gttgtgatga ctgacagtga actgatgcag ttaagcgaac aggttgggca ggcgctgaaa
    37501 gcccgtggcg caaccgtaac aactgccgag tcttgtaccg gtggttgggt agcgaaagtg
    37561 attaccgata ttgccggtag ctccgcctgg tttgaacgcg gatttgtcac ctacagtaac
    37621 gaagccaaag cgcagatgat cggcgtacgc gaagagacgc tggcgcagca tggcgcggtg
    37681 agtgaacccg tcgtggtgga aatggcgata ggtgcactga aagcggctcg tgctgattat
    37741 gccgtgtcta ttagtggtat cgccgggccg gatggcggca gtgaagagaa gcctgtcggc
    37801 accgtctggt ttgcttttgc cactgcccgc ggtgaaggca ttacccggcg ggaatgcttc
    37861 agcggcgacc gtgatgcggt gcgtcgtcag gctactgcgt atgcattgca gaccttgtgg
    37921 caacaatttc tacaaaacac ttgatactgt atgagtatac agtataattg cttcaacaga
    37981 acatattgac tatccggtat tacccggcat gacaggagta aaaatggcta tcgacgaaaa
    38041 caaacagaaa gcgttggcgg cagcactggg ccagattgag aaacaatttg gtaaaggctc
    38101 catcatgcgc ctgggtgaag accgttccat ggatgtggaa accatctcta ccggttcgct
    38161 ttcactggat atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta
    38221 cggaccggaa tcttccggta aaaccacgct gacgctgcag gtgatcgccg cagcgcagcg
    38281 tgaaggtaaa acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg
    38341 taaactgggc gtcgatatcg ataacctgct gtgctcccag ccggacaccg gcgagcaggc
    38401 actggaaatc tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg tcgttgactc
    38461 cgtggcggca ctgacgccga aagcggaaat cgaaggcgaa atcggcgact ctcacatggg
    38521 ccttgcggca cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc
    38581 caacacgctg ctgatcttca tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa
    38641 cccggaaacc actaccggtg gtaacgcgct gaaattctac gcctctgttc gtctcgacat
    38701 ccgtcgtatc ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa
    38761 agtggtgaag aacaaaatcg ctgcaccgtt taaacaggct gaatttcaga tcctctacgg
    38821 cgaaggtatc aacttctacg gcgaactggt tgacctgggc gtaaaagaga agctgatcga
    38881 gaaagcaggc gcgtggtaca gctacaaagg tgagaagatc ggtcagggta aagcgaatgc
    38941 aactgcctgg ctgaaagata acccggaaac cgcgaaagag atcgagaaga aagtacgtga
    39001 gttgctgctg agcaacccga actcaacgcc ggatttctct gtagatgaca gcgaaggcgt
    39061 agcagaaact aacgaagatt tttaatcgtt cgttattgat gataatagat aatgtgtgca
    39121 cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccaacactg
    39181 gcgtgaaaaa ttactctcac ctcgatcctc taacgaattg gctgcacact cattgactat
    39241 taaatctgtt tatcagcaaa acctgggtcg catctgcggc ccttttgctt ttttaagttg
    39301 taaggatatg ccatgacaga atcaacatcc cgtcgcccgg catatgctcg cctgttggat
    39361 cgtgcggtac gcattctggc ggtgcgcgat cacagtgagc aagaactgcg acgtaaactc
    39421 gcggcaccga ttatgggcaa aaatggccca gaagagattg atgctacggc agaagattac
    39481 gagcgcgtta ttgcctggtg ccatgaacat ggctatctcg atgacagccg atttgttgcg
    39541 cgctttatcg ccagccgtag ccgcaaaggt tatggacctg cacgtattcg tcaggaactg
    39601 aatcagaaag gtatttcccg cgaagcgaca gaaaaagcga tgcgtgaatg tgacatcgac
    39661 tggtgcgcac tggtgcgcga tcaggcgacg cgaaaatatg gtgaaccttt gccaactgtc
    39721 ttttcagaaa aagttaagat ccagcgtttt ctgctctatc gtggctatct gatggaagat
    39781 atccaggata tttggctaaa ttttgccgac tgaacgcata cggtatttta cttcccactc
    39841 aagaaaactt atcttattcc cacttttcag ttaccagccc ggcggttaag acacgctgga
    39901 gctggtggcg atatttcgtt agcttgattt caggataatt atgagcaaga gcaccgctga
    39961 gatccgtcag gcgtttctcg actttttcca tagtaaggga catcaggtag ttgccagcag
    40021 ctccctggta ccccataacg acccaacttt gttgtttacc aacgccggga tgaaccagtt
    40081 caaggatgtg ttccttggac tcgacaagcg taattattcc cgcgctacca cttcccaacg
    40141 ctgcgtgcgt gcgggtggta aacacaacga cctggaaaat gtcggttaca ccgcgcgtca
    40201 ccataccttc ttcgaaatgc tgggcaactt cagcttcggc gactatttca aacacgatgc
    40261 cattcagttt gcatgggaac tgctgaccag cgaaaaatgg tttgccctgc cgaaagagcg
    40321 tctgtgggtt accgtctatg aaagcgacga cgaagcctac gaaatctggg aaaaagaagt
    40381 agggatcccg cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc catacgcatc
    40441 tgacaacttc tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta
    40501 cgatcacggc gaccacattt ggggtggccc tccgggaagt ccggaagaag acggcgaccg
    40561 ctacattgag atctggaaca tcgtcttcat gcagttcaac cgccaggcag atggcacgat
    40621 ggaaccgctg ccgaagccgt ctgtagatac cggtatgggt ctggagcgta ttgctgcggt
    40681 gctgcaacac gttaactcta actatgacat cgacctgttc cgcacgttga tccaggcggt
    40741 agcgaaagtc actggcgcga ccgatctgag caataaatcg ctgcgcgtaa tcgctgacca
    40801 cattcgttct tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaatg aaaaccgtgg
    40861 ttatgtactg cgtcgtatca ttcgtcgcgc agtgcgtcac ggcaatatgc tcggcgcgaa
    40921 agaaaccttc ttctacaaac tggttggtcc gctgatcgac gttatgggct ctgcgggtga
    40981 agacctgaaa cgccagcagg cgcaggttga gcaggtgctg aagactgaag aagagcagtt
    41041 tgcccgtact ctggagcgcg gtctggcgtt gctggatgaa gagctggcaa aactttctgg
    41101 tgatacgctg gatggtgaaa cggctttccg tctgtacgac acctatggct tcccggttga
    41161 cctgacggct gatgtttgtc gtgagcgcaa catcaaagtt gacgaagctg gttttgaagc
    41221 agcaatggaa gagcagcgtc gtcgcgcgcg cgaagccagc ggctttggtg ccgattacaa
    41281 cgcaatgatc cgtgttgata gtgcatctga atttaaaggc tatgaccatc tggaactgaa
    41341 cggcaaagtg accgcgctgt ttgttgatgg taaagcggtt gatgccatca atgcaggcca
    41401 ggaagctgtg gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg gtcaggttgg
    41461 tgataaaggc gaactgaaag gcgctaactt ctccttcgcg gtggaagata cgcagaaata
    41521 cggccaggcg attggtcaca tcggtaaact tgctgcgggt tctctgaaag tgggcgacgc
    41581 tgtgcaggct gatgttgatg aggctcgtcg cgcccgtatt cgtctgaatc actccgcaac
    41641 gcacctgatg cacgctgcgc tgcgccaggt tctggggact catgtatcgc agaaaggttc
    41701 actggttaac gacaaggtgc tgcgcttcga cttctcacac aacgaagcga tgaaaccgga
    41761 agagattcgt gcggtcgaag acctggtgaa cgcacagatt cgtcgcaatt tgccgatcga
    41821 aaccaacatc atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga
    41881 gaagtatgat gagcgtgtac gcgtgctgag catgggcgat ttctccaccg agctgtgtgg
    41941 cggtactcac gccagccgca ctggtgatat tggtctgttc cgcatcatct ctgaatcggg
    42001 tactgctgca ggcgttcgtc gtatcgaagc ggtaaccgga gaaggcgcta tcaccaccgt
    42061 tcatgcagac agcgatcgct taagcgaagt cgcgcatctg ctgaaaggcg atagcaataa
    42121 tctggcggat aaagtgcgct cagtactgga acgtacgcgt cagttagaaa aagaactaca
    42181 acagcttaaa gaacaagctg ccgcacagga gagcgcaaat ctttccagta aggcaattga
    42241 tgttaatggt gttaagctgt tggttagcga gcttagcggt gttgagccga aaatgttgcg
    42301 taccatggtt gacgatttaa aaaatcagct ggggtcgaca attatcgtgc tggcaacggt
    42361 agccgaaggt aaggtttctc tgattgcagg cgtatctaag gacgtcacag atcgtgtgaa
    42421 agcaggggaa ctgattggta tggtcgctca gcaggtgggc ggcaagggtg gtggacgtcc
    42481 tgacatggcg caagccggtg gtacggatgc tgcggcctta cctgcagcgt tagccagtgt
    42541 gaaaggctgg gtcagcgcga aattgcaata atataagcgt caggcaatgc cgtggactcg
    42601 cttcacggca ttcgcattaa cgctatcgac aacgataaag tcaggttgaa gttgtgtata
    42661 tcggctaaac ttaggtttaa cagaatgtaa tgccatgact gcttacatgt aatgtgtttg
    42721 tcattgctta ctttttggcg ttatatgatg gataatgccg ggatacagag agacccgact
    42781 cttttaatct ttcaaggagc aaagaatgct gattctgact cgtcgagttg gtgagaccct
    42841 catgattggg gatgaggtca ccgtgacagt tttaggggta aagggcaacc aggtacgtat
    42901 tggcgtaaat gccccgaagg aagtttctgt tcaccgtgaa gagatctacc agcgtatcca
    42961 ggctgaaaaa tcccagcagt ccagttacta atctttccgc gtctcatctt tatcggtgag
    43021 acgcaccctc aaaatttctc cttcactcta tagtcttttc gctttactcc cgttcattca
    43081 acttaagtct ccattttttt gcattactgc tatctgccag acctccgttt ttctgtagat
    43141 aaaacactct ttttgacgtt tttacagact aattgagcgt gaagtgtgca aacgataaaa
    43201 gtgtgggaaa aattgtttga cttataagtc tcagaaagta atatgtgcgc cacgcagcga
    43261 cgatgagcga taaacaagtt cttcgaagca ctcgtaagag gcgtgtggtg aggtggccga
    43321 gaggctgaag gcgctcccct gctaagggag tatgcggtca aaagctgcat ccggggttcg
    43381 aatccccgcc tcaccgccat ttgcatccgt agctcagctg gatagagtac tcggctacga
    43441 accgagcggt cggaggttcg aatcctcccg gatgcaccat ctcttacttg atatggcttt
    43501 agtagcggta tcaatatcag cagtaaaata aattttcccg atgcatccgt agctcagctg
    43561 gatagagtac tcggctacga accgagcggt cggaggttcg aatcctcccg gatgcaccat
    43621 attctccgaa atcttcagca atgaaggtat cgaagaggta gcggaattaa ccgcaggcac
    43681 cagggatgat aacgttgctt tagcaacggc ccgaagggcg agccgcaagg cgagtaatcc
    43741 tcccggatgc accatctctt aattgatatg gctttagtag cggtatcaat atcagcagta
    43801 gaataagttt tcccgatgca tccgtagctc agctggatag agtactcggc tacgaaccga
    43861 gcggtcggag gttcgaatcc tcccggatgc accatattct ccgtaacctt cagcaatgaa
    43921 ggtatcgaag agatagcggt attaaccgca ggcaccagga aggataacgt tgcgccagca
    43981 acggcccgaa gggcgaacgt cagtgagtca tcctcccgga tgcaccatct tctccaaaaa
    44041 atttccttca aaaatatctc atttctaaat aactccttta tccatttcct gaattttctt
    44101 ctccttcgcc atcagcgaca aaatcaagca attacgctaa agtagcgtca tatttttcga
    44161 cttgcgagaa gaccatgtac gagcgttatg caggtttaat ttttgatatg gatggcacaa
    44221 tcctggatac ggagcctacg caccgtaaag cgtggcgcga agtattaggg cactacggtc
    44281 ttcagtacga tattcaggcg atgattgcgc ttaatggatc gcccacctgg cgtattgctc
    44341 aggcaattat tgagctgaat caggccgatc tcgacccgca tgcgttagcg cgtgaaaaaa
    44401 cagaagcagt aagaagtatg ctgctggata gcgtcgaacc gcttcctctt gttgatgtgg
    44461 tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac ggggagtgaa agcgccatcg
    44521 ctgaggcatt gctggcgcac ctgggattac gccattattt tgacgccgtc gtcgctgccg
    44581 atcacgtcaa acaccataaa cccgcgccag acacattttt gttgtgcgcg cagcgtatgg
    44641 gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga tttcggtatt caggcggccc
    44701 gtgccgcagg tatggacgct gtggacgttc gcttgctgtg agtgaagcgt tatcgctttt
    44761 ctcgttgttt gccagtagtt ttctcagcgc tacactatta cccggcaact ccgaagtcgt
    44821 gctggtggca atgttgcttt ccgggatcag tcatccctgg gttttagttt taacagcaac
    44881 aatgggtaat agccttggag ggttaactaa cgttatcctt gggcgtttct ttccattgcg
    44941 taaaacatcg cgctggcaag agaaagctat cggctggctg aaacgctatg gtgcagtcac
    45001 gctattatta agctggatgc ccgtggttgg cgatttactg tgtctgttag cgggatggat
    45061 gcgcatctcg tggggaccgg taatcttttt tttgtgcctt ggtaaagcgt tacgctatgt
    45121 tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg cactaattgt aggcctgcac
    45181 atatggtcac cattacagtt atgctaatta aaacgatttt gacaggcggg aggtcaattt
    45241 gatcccggac gtatcacagg cgctggcctg gctggaaaaa catcctcagg cgttaaaggg
    45301 gatacagcgt gggctggagc gcgaaacttt gcgtgttaat gctgatggca cactggcaac
    45361 aacaggtcat cctgaagcat taggttccgc actgacgcac aaatggatta ctaccgattt
    45421 tgcggaagca ttgctggaat tcattacacc agtggatggt gatattgaac atatgctgac
    45481 ctttatgcgc gatctgcatc gttatacggc gcgcaatatg ggcgatgagc ggatgtggcc
    45541 gttaagtatg ccatgctaca tcgcagaagg tcaggacatc gaactggcac agtacggcac
    45601 ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc gctacggcgc
    45661 gctgatgcaa accatttccg gcgtgcacta caatttctct ttgccaatgg cattctggca
    45721 agcgaagtgc ggtgatatct cgggcgctga tgccaaagag aaaatttctg cgggctattt
    45781 ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt ccttatctgt ttggtgcatc
    45841 tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt ttgagaaaac
    45901 cgagtgcggt atgtattacc tgccgtatgc gacctctctt cgtttgagcg atctcggcta
    45961 taccaataaa tcgcaaagca atcttggtat taccttcaac gatctttacg agtacgtagc
    46021 gggccttaaa caggcaatca aaacgccatc ggaagagtac gcgaagattg gtattgagaa
    46081 agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg aactgtatgc
    46141 gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg cgctgttacg
    46201 tggcggcatt gaatatattg aagtgcgttc gctggacatc aacccgttct cgccgattgg
    46261 tgtagatgaa cagcaggtgc gattcctcga cctgtttatg gtctggtgtg cgctggctga
    46321 tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc gttaactgga accgggtgat
    46381 ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg cgcagttccc
    46441 gttactgcag gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc aaacgctgga
    46501 tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat gaactggttg cctgcttcga
    46561 taatcccgat ctgactttct ctgcccgtat cttaaggtct atgattgata ctggtattgg
    46621 cggaacaggc aaagcgtttg cagaagccta ccgtaatctg ctgcgtgaag agccgctgga
    46681 aattctgcgc gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc gtcagcagga
    46741 aatggaaacc gctgataccg aaccgtttgc ggtgtggctg gaaaaacaca cctgacagaa
    46801 aagaaaaagg ccactcgtga gtggccaaaa tttcatctct gaattcaggg atgatgataa
    46861 caaatgcgcg tctttcatat actcagactc gcctgggaag aaagagttca gaaaattttt
    46921 aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata gcttcacagt cgatcatacc
    46981 cggatggaag cgcctgcagt tcgggtggcg aaaacaatga acaccccgca tggcgacgca
    47041 atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc agaaagaggg
    47101 atccataccc tggagcacct gtttgctggt tttatgcgta accatcttaa cggtaatggt
    47161 gtagagatta tcgatatctc gccaatgggc tgccgcaccg gtttttatat gagtctgatt
    47221 ggtacgccag atgagcagcg tgttgctgat gcctggaaag cggcaatgga agacgtgctg
    47281 aaagtgcagg atcagaatca gatcccggaa ctgaacgtct accagtgtgg cacttaccag
    47341 atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg tgacgtacgc
    47401 atcaacagca acgaagaact ggcactgccg aaagagaagt tgcaggaact gcacatctag
    47461 tcagtaaact atcttcacaa ttaattgaaa aaagccagtt caaatgaact ggcttagttg
    47521 tacttagtgc gcaccgccgc caccgccacc tgcgccaaat ggcggtttag caaaccacac
    47581 cagccccagc agtacgagga atatcccggc tgacatccag aagatctcat tggcggaaat
    47641 aatcagcccc tgattggtga tctgctgggc aatccagcct gatgcctgct gttgcgtcat
    47701 cccaagccct tccagttgac tgtacatcgc ctgggcattc gggttgaacg ggtttaccga
    47761 ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg tggttatcga
    47821 cgtgccgata gaccccgcca gcgttcgcgt aaagttagag aggctcgatg ccgccgccag
    47881 tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga agcaggccac
    47941 cgcaaacccc tggataaact gcggccaggc cgacgcgcca aaatccatac ctggttcaaa
    48001 ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg ttaccagccg
    48061 ccgcatatcc agtttatgcg cgaagcggcc gataatcggc gacaggatca ccggaataat
    48121 ccctaccggc gcagaggcca aacctgccca ggtcgccgtg taaccgtaga cctcctgcaa
    48181 caactgcggc agcagaacaa tagcgccgaa gtagagcata tacgcgaggc tgatacacaa
    48241 gcagccgatg gtgaagttgc gcgacttaaa caacgacaga tcgactatcg ggttatcgtc
    48301 ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg taaggatgat
    48361 aatttcctgt gatgaaaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct
    48421 gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc gtttggtgcg
    48481 ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc accgccacgc caatcggcac
    48541 gttgatgaag aatatccagc cccagtggta attatcgctg atataaccgc cgaggatcgg
    48601 gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt
    48661 ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcaacg gcccggcgac
    48721 aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc
    48781 ccacgacgca atagcaaagg cgatggtgga ccaaaggaac agtttcactt ccccgacgcg
    48841 ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat
    48901 tacccacgtt ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac
    48961 gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat
    49021 aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt
    49081 agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt
    49141 cacgcgcggt acttaccgcg accggtgttg aacgtacttt atttgccagt acctgaccgt
    49201 cacggttagt ggtattgacg ctcaccagtg tggacaaacc aatgcgcagc gggtattgct
    49261 ccagctgttt ctggtccagt tcgatacgca caggtagacg ctgaacgact ttgatccagt
    49321 taccggtcgc attttgcgcc ggaagcagtg agaatgcgct acctgtgccc atatccagac
    49381 caaccacttt accggtgtat ttcacatcat cgccgtaaat atccgtggtg atagtgaccg
    49441 gctgaccgat acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat
    49501 tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta
    49561 ccgcacggcg ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca
    49621 gccaggcgtt acgtacttcg gtggcagcct gttgcacggc aggctgatct tccagtttag
    49681 tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgcgcct
    49741 gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat
    49801 tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt tgcgagggcg attttctgca
    49861 cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc
    49921 tggaagccag tgcagttttg gctttttcaa acgcctggcg agcatctgtc gggtcgagag
    49981 tgaccagcac gtcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc
    50041 tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcggttt
    50101 cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa
    50161 agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct
    50221 gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc
    50281 ggctgagccg acctgcttgc caaaaaggcc agcatgttgc gatgctggcc ggtcattttt
    50341 tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca
    50401 caccgtcttg ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct
    50461 gatctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggtg
    50521 gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc
    50581 gatcgttatc gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac
    50641 gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa ttcagaaggc tgaatactgt
    50701 ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag
    50761 ccttcagcat tttattgcgg ttctccagca gcttgctttg catgtgcatg caaagacgag
    50821 tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt
    50881 gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat
    50941 agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt
    51001 atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga
    51061 tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagcgtgg
    51121 gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc
    51181 atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc
    51241 gggcattacc cacacgcagg cgcagcggca aatagcggaa gcaataattc gccgcgccaa
    51301 ccagtaaccc aggcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcttgg
    51361 attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat
    51421 agcgttacgc ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc
    51481 tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct
    51541 tcaacggcgg gataaccttg cagcaagccg ctgccagaga atgcccctat taccgtacca
    51601 aatacccacg atgaccatga actgaaggca atgccgatca tccagttctc gctccagcgg
    51661 cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa cctcatccgt caggccaaac
    51721 gcccacaggg cggttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg
    51781 ccatacaaca catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta
    51841 ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag
    51901 gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca
    51961 aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag
    52021 gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat
    52081 tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt
    52141 aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt tgcggcttcc
    52201 tgacgatgaa aacctcgcca ccagaccagt aagttaacta gggcaatcgt cgcgccagcc
    52261 agacaaacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag cgaaccggcg
    52321 gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg
    52381 tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgtacggt gagatccagc
    52441 accaggatgc cgataatcag cgccagtacg gaagtgtgac caaaccagat cgccagccat
    52501 gaaagtaata gcagcagcag accgaaagtt gtggtgtggt gcgatttacc cttatcggca
    52561 aaaccgcccg ccggacgagc gcccaacgct ccggcagctc ccgcaagtcc aaacagacca
    52621 atgacaccat cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat tgaggtccag
    52681 agaatgctga agttggcaaa ggtcaggcag cccagcaatg cgcgggtgcg gaggatctta
    52741 tcgctgataa acatgctgaa aacggaacct aacaactgtg ggtagttgag gtgggtttct
    52801 gatttcattt gtggcagacc acgccataat gctagcgcca tcagtgccat taacaccgag
    52861 gcaacccaaa agacggtgcg ccagccgccg aggtttgcca gtaacccggc aacggttcgt
    52921 gccagcaaga tccccaacag cagaccgctc ataatggtgc caaccacttt gccgcgttta
    52981 tccggtgaag ccagcgtcgc tgccagcgga accagaattt gtgccacgac tgagaataaa
    53041 ccggttaatg ccgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac
    53101 atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca
    53161 ccgaggggaa caagaaacag cagacctgct gcatagccca actgcgcggc ggtaacaata
    53221 aagcctgccg aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg
    53281 gcgtaatagt tactggctac cgcctgaccg gtggcgatag acatcagcac gatcagcgcc
    53341 gggctaagct catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg
    53401 gaaatcaata atagcggatg ttgatgaatg ggggcgaaag atagcttgtt gaaatgtgcg
    53461 atgtggtttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaacg
    53521 gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga gtgtattttg taggcatgat
    53581 aagacgcgcc agcgtcgcat caggcattgt gcacagaatg gcggatgagg caaaaacgac
    53641 ttatccgccc gaataaaaat tacttctgcg ctgccagcgc ctcattcacc cagccatcga
    53701 actgctgctg gtgggctttg atccaaccat caacatggcc ctgaatatcg ccttctgagg
    53761 ctttgccgtc atgcataatg gcgttctggg cgttaatatc tgccactggc aactgcataa
    53821 tggcaaaaag tttcgctgct gccgggtttt tctcggccca ggctttgttg gcaacgatat
    53881 gcatggtgct gaccgggaag ccataattcg caccattcgg cagtttggta tcggcgtttt
    53941 tatcgcccgg cagtgcggag aacggcacct gcaaccagac cacatctttc cctggcttca
    54001 gctcattact cacccagtac ggcgtccagg tgtaataaaa caccggtttg ccctctttgt
    54061 agcgactgat ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc gtcacggtgt
    54121 tggtcagttc atacgcggca agctggtggt tgatcgcacc ttcgcagccc cagcctgggt
    54181 tacagccggt taaatccgct ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg
    54241 gatctttcag ttgtgcgatg ttggtgattt tgtactggtc ggcggttttc ttatcgatca
    54301 ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca ataaaatttc ttatcgccac
    54361 cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt cacggcggtg aaggttgcat
    54421 cgccggaagc aagtgaggtg tagccaacgt tgtaatccac ttcgctgggt ttattgacgg
    54481 tataacctaa tttctccagt gcacgactga ccagcagcgt ctggaaggtt tcttcagtga
    54541 tggtgctctg aactggatta acagtaatgc ctttgcccgg cagatcggct gcaaaagttt
    54601 gtgtagagat aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc attgttattc
    54661 ctttttttgg cagggtgata atgcccggcg ttaccgggca agtgcagagt tacttaatga
    54721 atgggcgggt cagcagaccg acagggccag tggtgtacca gcgacggttg ccgcgactgc
    54781 gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat aatggcgagg atcacaatcc
    54841 cgacgccgcc aacggtggca aggcccatat ccagacgacc gataccgcga agtaccatct
    54901 gacccagccc gccgacggca atcatcgagg cgatgaccac catagaaaga gccagcatca
    54961 gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag cggtaactga actttgaaca
    55021 gcatctggcg cgggctggca ccgaatgagc gcgaggcttc aatcagatcc gccggaacct
    55081 ggttaatccc cagaatcgtc agacggataa tcggcggcag agcaaagatg atcgtcacca
    55141 ctacgcccgg cacgttaccg ataccaaaca gcatgacgat tggtaccaga taaacaaacg
    55201 ctggcgtggt ctgcatggca tcaagcagtg gacgaataat tttcgccgct cgcggacttc
    55261 tcgccagcca tatccccaac ggcaaaccga tgacgataca gaacagcagg gcggttaaca
    55321 ccagcgccag agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg
    55381 aaaccagcgt cgccacgccc attccgaccc cggaaatctg ccaggcgatg agagcgaaaa
    55441 cgataatcgc cactggcgct ggcataccca gcagcaattg ctggaaactg ttgaggatat
    55501 aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa atgggtaaca acccagtcga
    55561 tcccttcagt gacccaactg tcgagcggga tcagcgtttt atggaacgga tcgagaatat
    55621 taaaatgctc gacgtttggc gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag
    55681 tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg tccgccgctg
    55741 gcgtggtatc ccacggatta ttttgatcag ccattattta ccccctcacg atctaaagcg
    55801 cgcagcagca ttcctttcga aatgatgccg acatactgtt ggtcctcgtc gaccacgggc
    55861 accgcacagg gtgcctgtcc gacatgagag agcaactcgc taagaggcgt ttgtgcatcg
    55921 actgctaacg gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt taacgcggtt
    55981 ttaagcgaat cgatggagac tgcgccgaca aacttattac cgcgttcgat aacgtagcca
    56041 tattcgcgat cttcatcctg caataatttc agtgccgaac gtgggccgaa gccaggggtt
    56101 ttacgaatta agccattcgg tgtccggcgg gcaatatctt tcgcactgaa tacctgacta
    56161 atatcaacgc cacggaagaa ggtacggaca taatcattcg ccggattatt gagaatttca
    56221 tccggtgtgc cgacctgtac cacttcacca ttttgcataa tggcaattcg gtcgccaata
    56281 cgcatggctt catcgagatc gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt
    56341 aattttacca gctcatcctg catctcggtg cgaattaatg gatcgagcgc cgagaaggct
    56401 tcgtccatta ataaaatatc cggattaatc gctaacgcgc gggctaatcc cacacgttga
    56461 cgcatcccgc cagagagttc atccgggtag ctgtgggcat aattttccag cccgacctga
    56521 cgcagtgcat caagggcttt ttcccggcgt tcttcggcat taattccggc caattccata
    56581 ccgaacgcag tattgtccag cacggtcata tgcggcatta aggcaaagga ctggaagacc
    56641 atcgcaatct tttttctgcg cacctcacgg agtttggcat cagatatttt ggcaatatcc
    56701 acaccatcaa tcagcacttg cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc
    56761 attgtggatt tacccgagcc ggataatccc atgatgacaa atatctcgcc ttcttcaatg
    56821 gccagactgg cgtcttttac gccaagcgat agcccagttt tttccagaat ttgttctttt
    56881 gaaagtcctt gttcgatata tttgaatgct cgctgtggat gctcaccaaa tattttataa
    56941 aggtttttaa tttctaattt aattgccatg caatagaaag attcctgtta tttgtttatg
    57001 tcgatatatt acccaatgga aatagttact tttttctacc ctaacatact gagaatctga
    57061 ggcaacccct gatggcaaat gtggcatgaa tattgatgcg agcaaatcgc cggaatttcc
    57121 cgtgatataa gggctgagag caaatcgaga aaagttgata ttttttgtca aagatagcga
    57181 aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa cagagtatca gtgtagatca
    57241 ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa ttatttccct gtcgcgggta
    57301 gtgatatttt tgaaaataac accctcagaa attccagtct tcatcttctg tttcaaccgc
    57361 tttccccatc acataagagg agcctgaacc ggaaaaaaaa tcgtgatttt catcggcatt
    57421 cggcgaaagc gcggcgagga ttgccggatt cacttccgcc atttctgcgg gaaataaagg
    57481 ttcgtagccc agattcatca aagccttatt ggcgttgtaa cagagaaacg ctttcacatc
    57541 gtcagcccac ggggtttcgg cgtacagctc atcggtgtat tgcagctcgt tgtcgtagag
    57601 ttccagcaac aaatcgaagg cgaaactctt caactcttca cgttgtccca gagatatctt
    57661 ttccatgttt ttctgatatt tatagcctat gtagtaaccg tggactgctt catcacggat
    57721 aatcagacgg atcaggtctg cggtattggt cagctttccg cggctggaaa aatacatcgg
    57781 cagccagaaa ccggaataga acaaaaaaga ttcaagaaac acactggcga ttttcttttt
    57841 cagcggatca tcaccgcgat aatgttgctg aataatctga gcttttcgct gcaacggtgc
    57901 gttttcttca ctccaggcgt aggcggcatc gacatctttg gtctggcaca gcgtcgagaa
    57961 aatcgaactg taagagcggg catgaaccgc ttccataaag ctgatattcg ataataccgc
    58021 ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc gcgccgataa cattttgcag
    58081 cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt ctacgacagt
    58141 taatgtctgc caggcaggaa tatcgttcga cagcggcacc ttttctggta gccagaaatt
    58201 gctggtcagg cgattccaca cctccagatc tttatcgtca gatatcttgt tccagttgat
    58261 ggcgctgata cgtgagagtt tcatagatat tccttaaagt gcacaggaga cgcagccttc
    58321 aatttcagtg ccttccagcg ccatctgacg caggcgaatg taatagagcg ttttgatacc
    58381 tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg tatcggggaa
    58441 aaacagcgtc agcgacaaac cctgatcgac atggcgagtc gcttccgcgt aggtgtcgat
    58501 gatcttttct gcgccgattt cgtaagcatc ctgatacagc gccagatttt cgttagtcat
    58561 aaacggggca gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct ctactttcgc
    58621 cacaatcgga tgaatactcg acgtagcatg gttgatataa gagatagaac cggttggcgg
    58681 caccgcctga agattctggt tgtaaatgcc gtagcgcatc acgtcgtcgc gcagttgcgc
    58741 ccacatctca cgggtaggta acgtaatacc gctgcgggca aacagttcgc caactttcgc
    58801 cgttttcggc tgccagttac cttgcagata ttggctaaaa tattcaccac tggcataacg
    58861 tgactgtttg aacccggcga aggtttcacc gcgttcgcgc gccagcaaca tcgaggtacg
    58921 cagtgcgtgc caggtgatgg catagaaata gagattggtg aaatccagtg cttccggcga
    58981 accataagcg atgccttctc gcgccagata gccgtgtaaa ttcatctgcc ccagtccgat
    59041 ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg ctgcggatat gactcatatc
    59101 tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg cgggcaaaat cgggggaatc
    59161 catggtgtgc gcaatattca acgaacctaa attgcaggag atatcatggc ctgtgcgggc
    59221 atagtcgaga ttctcgtcat actctgaggc acagttgacc tgcaaaatct ctgagcagag
    59281 attactcata tttatgcgcc cggcgacagg gttagcacgg tttaccgtgt cttcatacat
    59341 gatgtaggga tagccggact caaactggat ttctgccagc cgctggaaga aatcacgggc
    59401 gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt gttcgctgat
    59461 ggcgatatcg gcaaacggct tgccataaac tcgctcaacg tcatagggcg aaaacagcgc
    59521 catctgcgca ttctcttttg ccagatggaa agtgatatcc gggatcacca cgcccagcga
    59581 cagtgtttta atgcggattt tttcgtcggc attttcccgt tttgtatcga gaaaacgcag
    59641 aatatcggga tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg cgccgagttg
    59701 gttggcatag gaaaatgcgt cttccagcat tttcatcacc ggaattacgc cagaagattg
    59761 attttcaata cgtttaattg gcgcgcccgc ttctcgcaga ttcgacagca aaaatgccac
    59821 gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc gcccggccaa tcgactccat
    59881 attgtcttca atacgcagca gaaaacagga aaccagttcg ccgcgctgct gcttaccgca
    59941 gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac agcatttcat cggtcagttg
    60001 caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc accatcgtga ctcgatcggc
    60061 aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac gtatagctgg tgtaaaactt
    60121 ccatgccccg aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg caaacagcgt
    60181 aatgacaaag tcgcgagagt agcgattaag aacgctttca tcatagtaac cttcgttgac
    60241 cagccagttc aggcgctgct gctggctact gaaggtcaca ctgttcggac gcacatgcgt
    60301 cgccataaag gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac ctgcgctatc
    60361 gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg tcaggcattc
    60421 tgcggttgtc gttgccaaaa ttcggttact cctttacgaa cgttttcgat atcgctttgc
    60481 gtacccatga gttcaaaacg gtacagccac ggcacgccgc atttccgggc aatcacatct
    60541 ccggcgcggc catacgcctc accaaagttg cgattaccag aagcaataac gccgcgaagc
    60601 aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct atcgtggcac cgcgccagcc
    60661 gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag gctcgtctac ctgaatccgt
    60721 tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat aaaacgctgc
    60781 gtgttttcgg agctgctgga gaagtagacg agctggctca tgcactggcc gcgtgtggcg
    60841 ctggatgcag acggttaatc atgtccggac ggaaaccaga ccagctaaga tcgccagcaa
    60901 tcactaccgg caactgacga aagccctgag cacgcaacgc ttccgctgct tcaggaacgc
    60961 gatcgacatt aatcatttca aaatcaaagc cccggttttc catcgcccgt ttggtggcgt
    61021 ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg catgattcgt atttccgttt
    61081 aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct ctctgttgat acagagatac
    61141 tagatgtagt tgaaaaaaga ttcaaccaca caatatatag caaatagtga tcgaaattac
    61201 cctagatatg agcgtgacgg gggaggggga tttttgtgat tcaccaggca aaaagaaacc
    61261 ccgaagacag gcttcggggt taaagacgcg tatttattat catttttgca ctacgatttg
    61321 cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac
    61381 cagggtcttt cacgaacaaa agaatccgcg cagccaacgg catcgcgtgc ggcttgctgg
    61441 acgcgagtac gaccatgcat tcgcgccctg gtttctttca gcaatgcctg agctttactg
    61501 cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgagg
    61561 ctgtcagcta attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg
    61621 cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga
    61681 ccataatttt cttaactgag gttcatcgcg gctttatgga cttttctgaa agcgttgtga
    61741 atgcgcaatc aggtaaggta ggcccgcggt tgataataac gaggatcaaa tatgtattta
    61801 cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc
    61861 cagaaagctt atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga
    61921 atgggacgta ccgcactggt aattcatcca acattaaaag aacgtagttc gacactggct
    61981 gaacccgctt ccgatattaa aacttgcgat cattatcaac aatttccgct ctatttagca
    62041 ggcgagcgac acgagcatta cggtatcccg catggcttca gttcgcgtgt tgcgcttgaa
    62101 cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg
    62161 cgaaaagaag agtcaggctt ttacctgctg gtaatggctg actttaaaca gtcggcggca
    62221 ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact
    62281 gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac
    62341 cggtgactgg aacgtcacat aagccaggat atcagccaga ttttttatcc agcccgacgg
    62401 gctaactttg cgtgccgcgc gcataaatag atcgcggtac ataccgtatg gccatgcaat
    62461 taagatgttc accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc
    62521 ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt
    62581 atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa
    62641 aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc tcattgccat ctgcattttc
    62701 gcatagcgta tatcgctggc tatgactatt caattagtga aaaattttta tttagtcagt
    62761 ttttgtagat aaaatattcc gtaacggctc gttttttgta cattgtggat ttttacatgc
    62821 tgatatgatt tattttcaat taaatcaatt agatatgacc ttgcctttaa tttcactaat
    62881 gaaagtttgg gggtgaggta acgctataag cgtagagaat tcggtgtatg tcgtgaccgt
    62941 gtcatttaca gcatttttga tgccagaaaa tgtacattaa cagcagaa
//
LOCUS       DAJTVE010000006        60734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000006 DAJTVE010000000
VERSION     DAJTVE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..469
                     /locus_tag="OO439_000373"
     CDS             <1..469
                     /locus_tag="OO439_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6314839.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            582..1376
                     /gene="panB"
                     /locus_tag="OO439_000374"
     CDS             582..1376
                     /gene="panB"
                     /locus_tag="OO439_000374"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6314840.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1388..2239
                     /gene="panC"
                     /locus_tag="OO439_000375"
     CDS             1388..2239
                     /gene="panC"
                     /locus_tag="OO439_000375"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6314841.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="OO439_000376"
     CDS             complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="OO439_000376"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6314842.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3701..4081
                     /gene="panD"
                     /locus_tag="OO439_000377"
     CDS             3701..4081
                     /gene="panD"
                     /locus_tag="OO439_000377"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6314843.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4085..5314)
                     /locus_tag="OO439_000378"
     CDS             complement(4085..5314)
                     /locus_tag="OO439_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6314844.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5378..5818)
                     /locus_tag="OO439_000379"
     CDS             complement(5378..5818)
                     /locus_tag="OO439_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6314845.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5923..6693)
                     /locus_tag="OO439_000380"
     CDS             complement(5923..6693)
                     /locus_tag="OO439_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6314846.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6690..7616)
                     /locus_tag="OO439_000381"
     CDS             complement(6690..7616)
                     /locus_tag="OO439_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6314847.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7725..8387
                     /gene="can"
                     /locus_tag="OO439_000382"
     CDS             7725..8387
                     /gene="can"
                     /locus_tag="OO439_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6314848.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="OO439_000383"
     CDS             complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="OO439_000383"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6314849.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9170..11560
                     /gene="gcd"
                     /locus_tag="OO439_000384"
     CDS             9170..11560
                     /gene="gcd"
                     /locus_tag="OO439_000384"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6314850.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="OO439_000385"
     CDS             complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="OO439_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6314851.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13323..13670
                     /locus_tag="OO439_000386"
     CDS             13323..13670
                     /locus_tag="OO439_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6314852.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13776..14642
                     /gene="speE"
                     /locus_tag="OO439_000387"
     CDS             13776..14642
                     /gene="speE"
                     /locus_tag="OO439_000387"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6314853.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14658..15452
                     /gene="speD"
                     /locus_tag="OO439_000388"
     CDS             14658..15452
                     /gene="speD"
                     /locus_tag="OO439_000388"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6314854.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15490..15852)
                     /locus_tag="OO439_000389"
     CDS             complement(15490..15852)
                     /locus_tag="OO439_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6314855.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="OO439_000390"
     CDS             complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="OO439_000390"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6314856.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18979..20736
                     /locus_tag="OO439_000391"
     CDS             18979..20736
                     /locus_tag="OO439_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6314857.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="OO439_000392"
     CDS             complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="OO439_000392"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6314858.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22556..24448)
                     /gene="aceF"
                     /locus_tag="OO439_000393"
     CDS             complement(22556..24448)
                     /gene="aceF"
                     /locus_tag="OO439_000393"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6314859.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24463..27126)
                     /gene="aceE"
                     /locus_tag="OO439_000394"
     CDS             complement(24463..27126)
                     /gene="aceE"
                     /locus_tag="OO439_000394"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6314860.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27287..28051)
                     /gene="pdhR"
                     /locus_tag="OO439_000395"
     CDS             complement(27287..28051)
                     /gene="pdhR"
                     /locus_tag="OO439_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6314861.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28592..29965
                     /gene="aroP"
                     /locus_tag="OO439_000396"
     CDS             28592..29965
                     /gene="aroP"
                     /locus_tag="OO439_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6314862.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30008..30862)
                     /gene="ampE"
                     /locus_tag="OO439_000397"
     CDS             complement(30008..30862)
                     /gene="ampE"
                     /locus_tag="OO439_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6314863.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30859..31410)
                     /gene="ampD"
                     /locus_tag="OO439_000398"
     CDS             complement(30859..31410)
                     /gene="ampD"
                     /locus_tag="OO439_000398"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6314864.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31498..32391
                     /gene="nadC"
                     /locus_tag="OO439_000399"
     CDS             31498..32391
                     /gene="nadC"
                     /locus_tag="OO439_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6314865.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32594..33034
                     /gene="ppdD"
                     /locus_tag="OO439_000400"
     CDS             32594..33034
                     /gene="ppdD"
                     /locus_tag="OO439_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6314866.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33044..34430
                     /gene="gspE"
                     /locus_tag="OO439_000401"
                     /pseudo
     CDS             33044..34430
                     /gene="gspE"
                     /locus_tag="OO439_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34420..35622
                     /gene="hofC"
                     /locus_tag="OO439_000402"
                     /pseudo
     CDS             34420..35622
                     /gene="hofC"
                     /locus_tag="OO439_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35657..36700)
                     /gene="guaC"
                     /locus_tag="OO439_000403"
     CDS             complement(35657..36700)
                     /gene="guaC"
                     /locus_tag="OO439_000403"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6314867.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36856..36900)
                     /locus_tag="OO439_000404"
     CDS             complement(36856..36900)
                     /locus_tag="OO439_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314868.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36925..37545
                     /gene="coaE"
                     /locus_tag="OO439_000405"
     CDS             36925..37545
                     /gene="coaE"
                     /locus_tag="OO439_000405"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6314869.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37545..38288
                     /gene="zapD"
                     /locus_tag="OO439_000406"
     CDS             37545..38288
                     /gene="zapD"
                     /locus_tag="OO439_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6314870.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38298..38495
                     /gene="yacG"
                     /locus_tag="OO439_000407"
     CDS             38298..38495
                     /gene="yacG"
                     /locus_tag="OO439_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6314871.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38595..38984)
                     /gene="mutT"
                     /locus_tag="OO439_000408"
     CDS             complement(38595..38984)
                     /gene="mutT"
                     /locus_tag="OO439_000408"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6314872.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39044..41749)
                     /gene="secA"
                     /locus_tag="OO439_000409"
     CDS             complement(39044..41749)
                     /gene="secA"
                     /locus_tag="OO439_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6314873.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41811..42323)
                     /gene="secM"
                     /locus_tag="OO439_000410"
     CDS             complement(41811..42323)
                     /gene="secM"
                     /locus_tag="OO439_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6314874.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42554..43471)
                     /gene="lpxC"
                     /locus_tag="OO439_000411"
     CDS             complement(42554..43471)
                     /gene="lpxC"
                     /locus_tag="OO439_000411"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6314875.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43572..44723)
                     /gene="ftsZ"
                     /locus_tag="OO439_000412"
     CDS             complement(43572..44723)
                     /gene="ftsZ"
                     /locus_tag="OO439_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6314876.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44784..46046)
                     /gene="ftsA"
                     /locus_tag="OO439_000413"
     CDS             complement(44784..46046)
                     /gene="ftsA"
                     /locus_tag="OO439_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6314877.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46043..46873)
                     /gene="ftsQ"
                     /locus_tag="OO439_000414"
     CDS             complement(46043..46873)
                     /gene="ftsQ"
                     /locus_tag="OO439_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6314878.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46875..47795)
                     /gene="ddlB"
                     /locus_tag="OO439_000415"
     CDS             complement(46875..47795)
                     /gene="ddlB"
                     /locus_tag="OO439_000415"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6314879.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47788..49263)
                     /gene="murC"
                     /locus_tag="OO439_000416"
     CDS             complement(47788..49263)
                     /gene="murC"
                     /locus_tag="OO439_000416"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6314880.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49317..50384)
                     /gene="murG"
                     /locus_tag="OO439_000417"
     CDS             complement(49317..50384)
                     /gene="murG"
                     /locus_tag="OO439_000417"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6314881.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50381..51625)
                     /gene="ftsW"
                     /locus_tag="OO439_000418"
     CDS             complement(50381..51625)
                     /gene="ftsW"
                     /locus_tag="OO439_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6314882.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51625..52941)
                     /gene="murD"
                     /locus_tag="OO439_000419"
     CDS             complement(51625..52941)
                     /gene="murD"
                     /locus_tag="OO439_000419"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6314883.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52944..54026)
                     /gene="mraY"
                     /locus_tag="OO439_000420"
     CDS             complement(52944..54026)
                     /gene="mraY"
                     /locus_tag="OO439_000420"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6314884.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54020..55378)
                     /gene="murF"
                     /locus_tag="OO439_000421"
     CDS             complement(54020..55378)
                     /gene="murF"
                     /locus_tag="OO439_000421"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6314885.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55375..56868)
                     /gene="murE"
                     /locus_tag="OO439_000422"
     CDS             complement(55375..56868)
                     /gene="murE"
                     /locus_tag="OO439_000422"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6314886.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56855..58621)
                     /gene="ftsI"
                     /locus_tag="OO439_000423"
     CDS             complement(56855..58621)
                     /gene="ftsI"
                     /locus_tag="OO439_000423"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6314887.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58637..59002)
                     /gene="ftsL"
                     /locus_tag="OO439_000424"
     CDS             complement(58637..59002)
                     /gene="ftsL"
                     /locus_tag="OO439_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6314888.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58999..59940)
                     /gene="rsmH"
                     /locus_tag="OO439_000425"
     CDS             complement(58999..59940)
                     /gene="rsmH"
                     /locus_tag="OO439_000425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6314889.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59942..60400)
                     /gene="mraZ"
                     /locus_tag="OO439_000426"
     CDS             complement(59942..60400)
                     /gene="mraZ"
                     /locus_tag="OO439_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6314890.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14523 a        16816 c        14952 g        14443 t
ORIGIN      
        1 tagtggaact tctgtgactg gttctacggt aaaaatgggt gaatatagtg cagaacaaat
       61 cagaaacggt gccacaccgg ttccttttga tatttcactt caaaactgcg ttcgtgtgac
      121 taatattgag acaaaattag tttcaacaaa ggttggtact gaaaacgggc aactccttgg
      181 taatactctc acaggtaatg acgcagcaaa aggagtcggt gtactcatag aaggtttagc
      241 aactagtaaa aatcctctaa tgacattgaa acctaatgat tcaaattctg tttataaaga
      301 ttacgacccg agaggcaaag acgatacaac aggaggagtt tacccggatc aagatacagg
      361 tataacatac cctctccatt tccaggccac gctacaacag gatggaacta taccaataga
      421 agctggtgag tttaaagcca ccagtacttt ccaggtaacc tacccttaat aagtccaccg
      481 caccgccatc atctggcggt gcggtaattg ataaatcatt gcccccacca atgtgactga
      541 taaaatgcca ggcaacacga catcatttat caggacacgt tatgaaaccg accaccattg
      601 cttcacttca gaaatgtaag caggataaaa agcgtttcgc gaccatcacc gcttacgact
      661 acagcttcgc gaaactattt gctgatgaag gacttaacgt catgctggta ggcgactcgc
      721 tgggcatgac ggttcagggt cacgactcca ccctgccggt gaccgttgcc gatatcgcct
      781 accacactgc ctccgtacgt cgtggtgcgc caaactgcct gctgttggct gacctgccgt
      841 ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt atgcgtgccg
      901 gtgccaatat ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta aaaatgctga
      961 ccgaacgtgc cgttcctgta tgtggtcact taggtttaac cccacagtca gtgaatattt
     1021 tcggtggcta caaagttcag gggcgcggcg atgaagcggg cgatcaactg ctcagcgatg
     1081 cattagcctt agaagccgct ggggcacagc tgctggtgct ggaatgcgtg ccgattgaac
     1141 tggcaaaacg tattaccgaa gcactggcga tcccggttat tggcattggc gcaggcaacg
     1201 tcactgacgg gcagatcctc gtgatgcacg acgccttcgg cattaccggc ggtcacattc
     1261 ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct gtgcggcagt
     1321 atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc cattaaggag
     1381 tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc gccgcctgcg
     1441 tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg atggccatat
     1501 gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta ttttcgttaa
     1561 cccgatgcag ttcgaccgcc cggaagatct ggcacgttat ccacggacct tgcaggagga
     1621 ctgcgagaag ctaaacaaac gtaaagtgga tttagttttc gccccttcgg taaaagagat
     1681 ctacccgaac ggtactgaaa cccatactta cgttgacgtt cccgggcttt cgaccatgct
     1741 ggaaggcgcg agccgtccgg ggcattttcg cggcgtttcg actatcgtca gcaagctgtt
     1801 taacctggtc cagccggaca tcgcctgctt cggtgaaaag gactttcagc aactggcgct
     1861 aatccgcaaa atggttgccg atatgggctt cgatattgaa attgtcggcg tcccgattat
     1921 gcgcgccaaa gatggccttg cgctcagttc ccgtaatggt tatctgacgg cggaacaacg
     1981 caaaattgcg cccggtctgt acaaagtttt aagttcaatt gccgacaaat tgcaggctgg
     2041 cgagcgggat cttgatgaaa ttattgccat tgcagggcaa gaactgaatg aaaaaggctt
     2101 ccgcgccgat gatattcaga ttcgcgatgc cgacacgttg ctggaagtct ctgaaaccag
     2161 caaacgagct gtaattctgg tagccgcctg gcttggcgat gcccgcctga tcgacaacaa
     2221 aatcgtcgaa ctggcgtaat gcttttactc taaataattc aagttgcagg aaggcggcaa
     2281 gcgagtgaca aattcgtcag gaacgaattt gcccagccgc aggctggcct tcggtgagag
     2341 acatggatgt ctctcattaa tccccaggag cttacataag taagtgactg gggttagcga
     2401 acgcagacgc agcacatgca atttaaagta tgacgagtat aactggcgct acggctgatg
     2461 gcgccaatta ttaatttgcc ccacgtcatg gcgatggcgt ctgtaagcaa ttattcataa
     2521 cgcagacaac ttagttaatc agcctatcaa tctcagcaag agataaattt gtcatctctg
     2581 caacgtcttc ccgagacatc cctttactca gaaaacgctg ggcgaattct tgcctttcct
     2641 cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac tgcgccagcg
     2701 tcatcataga ctcccctccc gtttccctgt ctctcagcac accataaaac aaatccgctt
     2761 gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga cttccgctag
     2821 tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag tcgcgctggc
     2881 gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg atttcgtcat
     2941 ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt cgtgccagct
     3001 ccggcgagta aaatatatca aaccagcaca ttgagagcgg ataaggtgtg gcctcgccct
     3061 gataaaacag tatcggcacc accagcggca gcttatcgtg atccgcctcc agatgccggt
     3121 gcatggcagc aatagaataa cgcatcatgc gaaagaccat tttcttatcc ggcttgcttt
     3181 ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg gaatagagca
     3241 cgtccgtgct gtgtcctttc aggctctctt caatgaaact ccccgactct aaatgaagcg
     3301 tgttgaggtc acaaagttct cgtaattcca ctggcaaatg tatctccaga aagtcgcgag
     3361 ccgtctccgc gtgcattaaa aattgtttaa acactgcatc atgcggtgtg gtactcggtg
     3421 catccatctt ccctgccttc cgtgttgtct tgcgtgaagg cagtatccag tcaaggcgtg
     3481 ccatcgtcag ccacctcttt agtgtttacc gagcagcgtt cagagaaatt tcaattggtt
     3541 acagccgtta catggcacgc ttcggcacgg tacgcaaact gctgatgtaa atgccgtcag
     3601 atgagtagac actaaacaaa aatcgggcaa tactgcgtga gaatttcctc aaagcaggca
     3661 tcgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca cgatgctgca
     3721 gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag gttcttgcgc
     3781 cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca ttgatatctg
     3841 gaatgtcacc aacggcaagc gtttctccac ttatgccatc gcggcagaac gcggttcgag
     3901 aattatttct gttaacggtg cggcggccca ctgcgccagt gtcggcgata ttgtcatcat
     3961 cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca acgtcgccta
     4021 ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac aggttgcttg
     4081 attgttatcc ctgcggctgg ttactcacca gccgcgacat cgtctccagc gaatccgttc
     4141 ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact ttgcctttca
     4201 tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct ttgtcattaa
     4261 atccgccaaa cggatacgaa agataccaga catgcggatt aaattgcgcc agagcgcggc
     4321 gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat atgggtcggc
     4381 gatagccatc cacccgatgc aaaaaatggg tatgtgactg aaaatcaaaa acatcgcgaa
     4441 tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tgggttccac ttctgcgggt
     4501 gacgtttgat gcgcgaggta acaacaaacg ccgtcgcctt catgccatat tgtttcaata
     4561 caggatacgc atagcggctc accgacttga ggccatcatc aaaggtaatc accaccgctc
     4621 gcgcagggag attgatctta ttcttcacgt aaccttccag ctgcgccatg ctcagtgtcg
     4681 cgtatcccct gtcgcgcagc caggccattt ggttattgaa agcgcgtacc gaggtggtcg
     4741 tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga taagttagca
     4801 ccggaaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt cgatcgccaa
     4861 tacggacctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa atcgggtagc
     4921 gcaaattgtc cgccaatacc ccaaatggcg cactgcccac actcggcgcg ttatagaccg
     4981 gcgtatcttt ccaggtaact aagttctgat tactcagcgg cttgttgagg tcacccaaac
     5041 cgtcttcaac tttttgtcgc ccctgaaccg gctcgagatg ccctttatcg ataaaccctt
     5101 taccaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg gcaatgattt
     5161 gtccggcccg aatattcccc acggtcacca tcttgtcgcc aatttgcgcc cagaccgcag
     5221 cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt gcggtaaaca
     5281 gcatcagcag gagaataaca gcttgtttgt acatgaaaaa ataaccgagg gtgaaacaac
     5341 gcttttattg tagcaaaagc gctataagaa tcgtgggtta atactcatac aaattatgtt
     5401 tgaggacgaa aggcggattt ttttgttgtt ggtgccaaag actactcacc tccggcccac
     5461 ccagttcgac aatacgctcg cgaaactctg aactggtcat ggtttcacgg caagccatca
     5521 tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag tgtttgtggc
     5581 ttaataatga agccacgcgg tacggtggcg ctccggctat atcggttaag aagatgacac
     5641 cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc atattagagc
     5701 ttaacccacg ccagaaattc acggcccggc actgaagaag cgccccatgt tttttctcca
     5761 gcgcatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa cctaacatcg
     5821 ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg gtgatagccg
     5881 tcaaaactcc tctcccccaa atccagagga gaggaaatag ccttagctac gcaaaccacg
     5941 tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga ccaccagtac
     6001 gccaaaggta gtgaccagcg gaacatcatt gataccgagg aagccgtagc ggaaaccact
     6061 gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg gcaacaaagt
     6121 cagtgagtaa aagaccccac ccaaatacgt gagtggcgtt aacacaaagg ttggcaccag
     6181 gctgatgtca tcgaacgttt tggcaaacac accgttcagc aaacccgcaa gggagaacaa
     6241 caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct gaaacggcac
     6301 aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg ccacgccacc
     6361 gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca gctcttcaat
     6421 attacgctgg aacttggcac caaaaaatga tgacgcaacg ttggcgtagg cattggtgat
     6481 caccgacatc atgatcagcc ccggtacgat gaactgcata tagctgaagc catgcatatc
     6541 gccaatacgc gaaccaatca ggttaccgaa gataataaag taaagggtca tggtgatgac
     6601 tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt tcgcccagat
     6661 gctttttagc gccacccagt aaagatgcat catgcgcgat ctccttgttt ttcattaacc
     6721 agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa tacctgaatg
     6781 ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgcag cacttcaact
     6841 tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg taacggactt
     6901 ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag cgccttcatc
     6961 gaggtatttt ccaccagctc accgtgttga ataatgccga tattgcggca cagcatttct
     7021 gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt taaatccttc
     7081 aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcggtcgg ttcgtcgaga
     7141 atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg cttcatcccg
     7201 ccagataaca tacgcgcacg ttcgttgcgt tttccccata gatcgagttg tttaagatat
     7261 ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc ctgattcacc
     7321 acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac cagtcccaac
     7381 tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct gacccgcccg
     7441 gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc ggccccgttc
     7501 ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc acgaagcgcc
     7561 tgaacgccgc ctggataggt ttttttaagc tgttgaagtt ccagtgcaat ggtcataaat
     7621 ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg gagttgccct
     7681 atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa gacatagata
     7741 cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat cccgggtttt
     7801 ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt tccgacagtc
     7861 gcgttcctgc agaacgttta accggtcttg agccgggcga actctttgtt caccgtaatg
     7921 ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag tatgcagtgg
     7981 atgtactcga agttgaacac attattatct gtggccacta cggttgcggc ggcgtacaag
     8041 ccgccgttga aaacccggaa ctggggctta tcaacaactg gctgctgcat atccgcgata
     8101 tctggttcaa acatagctca ttgctcggcg aaatgccgca agagcgccgt ctggatacct
     8161 tgtgtgaact gaacgtcatg gaacaggttt ataacctagg ccactccacc attatgcaat
     8221 cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcctacggc attcacgacg
     8281 gcttgctgcg tgatctggat gtcacagcga ccaaccgcga aacccttgag caacgttacc
     8341 gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa tgccatgccg
     8401 gatgcaacac atccggcaac ttcacactta ctcgtcccgc agaatcactt tgccgatata
     8461 cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa cccaccacaa actcatccgg
     8521 gatcgagaaa ccgataaatt ctaccgggac gttcacttca cgacgggacg gtttatccag
     8581 cagcgtacaa atcgccagcg acttcggttc gcgcaggctt aagatctcac gcactttcga
     8641 cagtgtattc cccgagtcga tgatatcttc aacaatcagc acgtccttgc cacggatatc
     8701 ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc taccgtagct
     8761 ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca ggtccgccat
     8821 aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc tgtctttgta
     8881 acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg cttcggggat
     8941 cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca aatcacttcg
     9001 cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca gtaaaactat
     9061 tcattcgcgg aatctgttaa taaagctcga attgtgatga cggtcacaca tgttaaaacc
     9121 tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta tggcaattaa
     9181 caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag cgctttgcgg
     9241 gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct ggtactaccc
     9301 tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggtgcagta aacgcgccgc
     9361 gctttggcta tacgcagccc tgctgctcgg caccatgatt tggggcgtct gggaagttgg
     9421 tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg gcatctggct
     9481 gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag ttgccgcact
     9541 ggtggtggca ctgctgatta gcggtagtat cctgacctgg gccggattta acgatccgca
     9601 ggaaatcaac ggcaccttaa gcgccgatgc cacacctgcg gaagctatct cacccgttgc
     9661 cgatcaggac tggcctgcct atggtcgcaa tcaggaaggt caacgctttt cacctctgaa
     9721 acaaattaac gccgataacg tccataatct gaaagaagcc tgggtgttcc gtactggcga
     9781 tgtgaagcag ccgaacgatc cgggcgaaat caccaatgaa gtgacgccga ttaaagtggg
     9841 cgacaccctt tacctgtgta ccgctcacca gcgcctgttt gcgctcgatg ccgccagcgg
     9901 caaagagaaa tggcattacg atcctgagct gaaaactaac gagtctttcc agcacgtaac
     9961 ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg aagtgatggc
    10021 ggattgcccg cgtcgtatca ttcttccggt caatgatggt cgactgattg cgattaacgc
    10081 tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc gtgctcaatc tgcaaagcaa
    10141 tatgccagac accaaaccgg gtctgtatga accgacttcg ccaccgatta tcaccgataa
    10201 aaccatcgtg atggccggtt cagttaccga taacttctca acccgcgaaa cgtctggcgt
    10261 gatccgtggt tttgatgtca acaccgggga gctgctgtgg gcttttgatc ccggcgcgaa
    10321 agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcgc caaactcctg
    10381 ggcaccagcg gcctatgacg cgaagctgga tctggtctat ctgccgatgg gcgtgaccac
    10441 gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca gctcgattct
    10501 ggcgctgaat gccactaccg ggaaactggc gtggagctac cagaccgttc accacgacct
    10561 gtgggacatg gatcttccgg cacagccgac gctggcggac atcaccgtta atggtcagaa
    10621 agtgccagtt atttacgctc cggcgaaaac cggcaacatt tttgtgctcg atcgtcgcaa
    10681 tggcgaactg gtggtttcgg ccccggaaaa accggtgccg caaggtgctg cgaaaggcga
    10741 ttacgttact ccgacccagc cgttctctga actgagcttc cgtccgaaga aagatttgag
    10801 cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg tgatgttcca
    10861 ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggcacgc tggtcttccc
    10921 gggtaacctg ggaatgtttg aatggggcgg gatttccgtt gatccaaatc gtgaagtggc
    10981 aattgccaac ccaatggcac tgccgtttgt ttcgaaactg atcccacgcg gtccgggtaa
    11041 cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca ttcagccaca
    11101 gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccgtttg gtctgccgtg
    11161 taaacagcca gcatggggtt atatctcggc gctggatctg aaaatcaatg aagtggtgtg
    11221 gaagaaacgt attggtacgc cgcaagacag tatgccgttc ccgatgcctg taccggtgcc
    11281 gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta acgtgctgtt
    11341 tatcgctgct acggcagata actacctgcg cgcttacaac atgagcaacg gtgaaaaact
    11401 gtggcagggc cgtttaccag cgggtggtca ggcgacgcca atgacctatg aagtgaatgg
    11461 caagcagtat gtggtgatct ccgcaggcgg tcacggttca tttggtacga agatgggcga
    11521 ctatattgtg gcttatgccc tgccggatga tgtgaaataa ccataatgcc cggagagatc
    11581 cgggcatatt tccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    11641 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc gttgtgatta
    11701 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    11761 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcat gccgtgaata
    11821 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    11881 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    11941 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    12001 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    12061 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    12121 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtcaggcaa agcaccggag
    12181 gcactaatag caatcggctg aatccgcatt accggatgcg gcttgtcaaa cggcgcaatc
    12241 gccatcccca tctggctgac cggtagcgtc accagatcaa agggtttgtt gtcgttaacc
    12301 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgctcac cttcaccggt
    12361 tcaggtagca gaccaccgtc gctggcaatc acatacagcg ggcgattgtc gctggtggcg
    12421 aaattgagcg agcgggcgtt acagccattg agcaaacgca ggcgcagcca gccacgcggg
    12481 gcagcgtgtt gtgggtagat tgcaccgttg gtcagcagcg tatcgccaaa ccagcccacg
    12541 gcggcggtca tcacatccag ttgataatca atctgcccat cagcgttaaa ttttttatcc
    12601 tgaacaatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    12661 tcgtcatctt caattaccac cagcccagcc aaccccatcg ccacctgtcg cccggtcttg
    12721 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    12781 gtacgtttgc cgcctggcgg aataattccc tgcgggccgc cgtcgacgtc acccggtact
    12841 tccagcccgt gccagtgcaa cgtcgtctct tccgccagtt ggttatagat atcaaccgtt
    12901 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    12961 gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc caatagttaa ctgaatgcga
    13021 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    13081 actgcgcggc tccacagcgg caaggctgaa gccacaccca gcgcgacgga atattttaag
    13141 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    13201 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    13261 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    13321 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtc tgcgcaaaca
    13381 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    13441 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    13501 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    13561 ccctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaagt
    13621 gcaaagccct ggcccgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    13681 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    13741 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    13801 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    13861 agaccgatca ccaagatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    13921 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    13981 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    14041 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    14101 tcgatgcggg tatcgtgtcg ttctgccgtc agtatctgcc caaccataac gccggtagct
    14161 acgacgatcc gcgctttaag ctggtgatcg acgatggcgt caatttcgtt aatcaaacca
    14221 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagtc
    14281 tgttcacctc agcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    14341 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc tatcgcaaac
    14401 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    14461 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    14521 ttattcaggc gcgttttctc gcttctggcc tgaaatgccg ttattacaat ccggcaatcc
    14581 atacggcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    14641 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    14701 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    14761 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    14821 cctgttccat tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg
    14881 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    14941 cagaacaccc cggcccactg ccagaaacgg tcgttgccca tctcgataaa agtcatattt
    15001 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    15061 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    15121 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttacc cgcgacatta
    15181 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    15241 atatgaaggc gctgtatgac atggtggatg tgaacgtcta tcaggaaaat atcttccata
    15301 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    15361 atttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    15421 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    15481 cagatttatt tacttctgct gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc
    15541 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatccctt cttccatttc
    15601 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag
    15661 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    15721 ttgttccact tcatcaagca aagccaggtt ttctttcacc tcttcattaa accagtgccc
    15781 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    15841 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgctat
    15901 tgatgtaatt attgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    15961 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    16021 tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    16081 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgcaacgta
    16141 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    16201 tgcagaagcc aggaagacat tcgcgccagt ccccagacgg ttcgggaagt tacgggtaga
    16261 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagagaaca
    16321 gcccgggatc tcgatacgag ccccgctctt accgaagacg ctgtagtagc cttcttcagt
    16381 caactgtgcg gcgtccatgc gggttggcgg tgccacccac aggcgggtcg gcaactggcc
    16441 tttgtgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttag tcatgcaaga
    16501 accgataaac acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gtgcgtcgtc
    16561 cgggtcgttc ggtgcacaga ggattggctc tttaatatcc gccagatcga tgtcgatcac
    16621 tgccgcgtat tcagcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    16681 catgccctga atacgacgtt cgagggtacg acgatcgcca taaccttccg cgatcatcca
    16741 cttcagcagg acgatgttag agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    16801 agtacaacca gcggcagaac gctcagcgga cgcatcggtc agttcaaatg cctgctcaac
    16861 tttcagatcc ggcagacctt caatttccag aatgcgacca gagaagatgt ttttcttgcc
    16921 tttcttctca acggtcagca gaccttgttt gatcgcatac agcgggatcg cgtgtaccag
    16981 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    17041 catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg
    17101 gaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    17161 cagcagcata cggttcagcc aggagtggat tacgccgtca cccggacgca gcgacacacc
    17221 accacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    17281 atacgccgcg gtatggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    17341 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgtgaac ctacagaagt
    17401 cattttcggt tcgcagtacg cgcccggacg aatacctttc acaccgcagg cacgacctac
    17461 cattttctga gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    17521 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    17581 aatcagcgga atacggccac cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    17641 gaaggtcgcc agcagttcgc cggtttcgtg gttacgcact tcacctttgt acgggtaaac
    17701 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    17761 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    17821 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttcgtggc
    17881 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    17941 ctgttgcaga gcttcgattt gcttgatcgg gccaacaaca cctggctggt ctggctcaat
    18001 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgcgacca
    18061 cgcatccggt gccggagaaa ggtcatcagt gttggtttcg ccagtgactt tgaagacggt
    18121 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    18181 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
    18241 gttatcgaac atcagcagtg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
    18301 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tacccagcag
    18361 ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttag cgatagcagc
    18421 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
    18481 tagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    18541 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    18601 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    18661 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    18721 acgggcagaa tagcaggatt ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa
    18781 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    18841 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    18901 ccgcctcttt atactcggat taacagcacc tgcgggtggc agttcgccga ccattgcgat
    18961 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcacta
    19021 atttgcagtg ccgggctttg tgccgcctct gccgggctat atataaaaag ccgcacagtg
    19081 gaagcgcctg tggaaacgca atcgacacaa cagactgcgc ctgacatcac cgcagttacg
    19141 cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgtcaaatc cgcattcagc
    19201 actgcacaaa tcgatcaatg ggttgcgcct gtcgcgctgt accccgattc tctgctttca
    19261 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    19321 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    19381 gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg cgaaaacccg
    19441 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    19501 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    19561 aaagttatta ccacaacgaa gaaagctgta ccggtaaaac agacagtcac ggcacccgtc
    19621 ataccatcca ataccgtttt aactgccaac cccgtcatta cagagccggc aacaaccgtc
    19681 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    19741 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    19801 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    19861 tacgcactat ttagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    19921 gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg gcaacacaac
    19981 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    20041 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    20101 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag
    20161 ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    20221 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc acagcggttt
    20281 aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    20341 ctgacccagc aacaaaagga cgccgctcgt cagcgttatc aatcggcctc tcctgagcag
    20401 cgccaggcgg ttcgcgagaa aatgcagact aacccacaga accagcagcg aagagaggca
    20461 gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtgtttaa ggaaaaagta
    20521 cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat acaatcggca
    20581 tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg agagccgccc acaacgtcta
    20641 aacgacagta accatactgt caggctgaat aacgagcaac ggtcagcagt acgcgaacgt
    20701 ctctctgagc gcggagcaag gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg
    20761 tggttagccg cttttttaat tgccggatgt tccggcaaac gaaaaattac ttcttcttcg
    20821 ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc aggcccacag
    20881 actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca
    20941 tttcgattgc cagaccgatt tcacccagca gctcaccgcc gttagtaccg acaatcgcac
    21001 caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg tctgcgcagt
    21061 cggaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca tagctgatgc
    21121 ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt tcggtatagg
    21181 cgatagacgg gataactttc ggatcgaagt agtgtttttt accggcgata acttcagcgg
    21241 caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccaacg atatcgccga
    21301 tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg aaaccacggt
    21361 cgtccacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc acacgaccaa
    21421 tcgctaccag cacggcgtcg taacgctgcg gttcagcggg tgcttttttg ccttccatcg
    21481 tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt
    21541 tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca gctgccggga
    21601 taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc
    21661 ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt acttctttca
    21721 gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata aacggcagtt
    21781 ggatcgggcg agaacccgct gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt
    21841 tctcaccttc aacttccagg gtgttagccc cggtgaattt acccagaccg ttgaccactt
    21901 tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg atcactttct
    21961 ctttccaagt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt
    22021 cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct ttagaaggga
    22081 tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg attacggttt
    22141 ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct gccccaagta
    22201 ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta tctccggcgg
    22261 tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac cccagtcact
    22321 tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc agaaatccat
    22381 agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca
    22441 aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg ctaataacct
    22501 caatacattc atgagattac cagaaaaaag ccggccgttg ggccggctct tttacttaca
    22561 tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat
    22621 cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat cggcagcatc agacgcggca
    22681 cgaactcttt accattccac accggctcca tcgcggactt ggaaacgccg aggatagcca
    22741 cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc caggccgccg atgctggaga
    22801 tggtgaagca accgccctgc atttcgcccg cagtcagctt accgtcacgc gctttcttag
    22861 aaatagtcat cagctcgcga gacagctcga tgatgccttt cttgttgacg tctttgaata
    22921 ccggaacaac cagaccgttc ggggtatcca ccgccacacc gatgttgatg tatttcttca
    22981 gggtcagacg ctgaccgtct tccgacagcg aactattgaa gcgaggcatc tgctcaagag
    23041 ctgcagcaac ggctttcatg atgaagacaa ccggggtgag cttcacatcc agcttacgtt
    23101 tcgccgcttc ttcgttctgc tgtttacgga acgcttccag ctcggtgata tcggttttgt
    23161 cgaagtgggt aacatgcggg atcatcaccc agttacggct caggttagca ccagaaattt
    23221 tctggatacg gcccagttcc acttcttcga tttcgccaaa cttgctgaag tccaccttcg
    23281 gccacggcag catgccaggg ataccaccgc cagtcgccgc cggagctgct tctgcacgtt
    23341 tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag gatacgacct ttacgaccag
    23401 tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc cagacggcgg atcagcggag
    23461 tcgcgtgaac gtaagcgtcg ttttcagcaa attcagattt gccttcggct ttcgcagccg
    23521 gagctgctgc cggggcttca gctttcgctg ccggagccgg cgctgccgct tcctgtttcg
    23581 caggagctgc cgcaggcgct gcgccttcaa cttcgaagat cataatcagc gagccagttt
    23641 tcactttatc gccaacgttg actttcagtt ccttcacgac gcctgcaaac ggagccggga
    23701 cctccataga agctttatcg ccttctacgg tgataagcga ttgttcagcg gcaactttgt
    23761 cgcccacttt caccatcact tcggtcactt caacttcgtc accgccgata tccggaacgt
    23821 taacttcttt cacgccagcc gctgatgcag gggccgctgc cggagctgct tcctgtttag
    23881 ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa gaccataatc agcgagccgg
    23941 tagacacttt gtcacccacg ttcactttga tctctttcac ggtgccagca aacggagccg
    24001 gaacttccat agaagcttta tcgccttcta cggtgatcag cgactgttca gcttcaactt
    24061 tatcgccaac tttcaccagg atttcggtca cttcaacttc gtcgctgccg atatccggaa
    24121 cgttaacgtc ttttgccgcc gcagccgcgg gtgctgctgc cggagctgct tctttcttct
    24181 cttctgcctg agcaggtgca gcgtctgctg caccgtcggc ggaatcgaaa atcataatca
    24241 gtgcgccggt ctgggtttta tcgccaacag agactttgat ctctttaacg atacccgcct
    24301 gcggagacgg aacttccata gaggctttgt cgccttctac ggtgatcagc gactgttcgg
    24361 cttcaacttt gtcgcccact ttgaccagga tctcggtgat ttcaacttca tcagccccga
    24421 tgtccggtac tttgatttcg atagccatta ttcttttacc tcttacgcca gacgcgggtt
    24481 aactttatct gcatcgatgt tgaatttggc gattgcgtca gcaaccactt tcttatcgat
    24541 ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg acataagaag catcaacttc
    24601 gaagtggtga cgcaggttct cacggctgtc ggaacgaccg aagccatcag tacccagtac
    24661 gcggtagtcg tcagccggta cgtaagtacg gacctgctca gcgaacagtt tcatatagtc
    24721 ggtagatgcc actgccggag cgtcgttcat cacctgagcg atatacggaa cgcgcggagt
    24781 ttccagcgga tgaagcatgt tccagcgttc acaatcctga ccatcacgcg ccagctcggt
    24841 gaaggaggtc acgctataaa cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc
    24901 cgcttcacgg acgtgacgca ggatagaacc ggagcccagc agctgaactt tacctttgct
    24961 accttcaatg gtttcgagtt tgtagatacc tttacggata ccttcctcag caccttccgg
    25021 cattgccggc atgtggtagt tttcgttcag cgtagtgatg tagtagtaaa cgttctcttg
    25081 tttttcaccg tacatacgct ccagaccgtc atgcatgatg acagcaactt cgtaagcgta
    25141 agccgggtcg taagagatac agttcgggat agtcagcgac tgaatgtggc tgtgaccatc
    25201 ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa
    25261 gccacgcgct tgctggtcgc cagccgccca gcacagatcg ccgatacgct ggaagccgaa
    25321 catcgagtaa tagatgtaga acggaatcat cggcagattg ttggtgctgt aagaggtcgc
    25381 cgctgccagc caggaacacc ctgcgcccag ctcgttgatc ccttcctgca gaatctgacc
    25441 tttctcgtct tctttatagt aagcaacctg ctcgcggtcc tgcgggatgt actgctgacc
    25501 gttcgggctg taaataccaa tctgacggaa cagaccttcc ataccgaaag tacgcgcttc
    25561 gtcggcgatg atcggtacca gacgatcttt gatcgacttg ttcttcagca ttacgttcag
    25621 ggcacgaacg aaagcgatag tggtagagat ctctttgctc tgctcttcca gcagcgcgcc
    25681 gaagtcttgc aggctcggca gctcaagctt ctcggtgaag ttcggctgac ggcttggcag
    25741 ataaccgtgc agtttctgac gctgtgcgtg cagataggta tgctcttcag aaccttccgg
    25801 gaaggtgatg tacggcagtt tttcgatatc tgcatcagac accggcacat tgaaacggtc
    25861 gcggatgtga cgcacgccgt ccatgttcat tttcttaacc tggtgcgcga tgtttttacc
    25921 ttcagccgcg tcgcccatgc cgtaaccttt aatggtatga gcaaggatta ctgtcgcttt
    25981 gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt ttcttcggat cgtgaccacc
    26041 acggttcagt gcccagatct gctcgtcagt ccagtctgca accagtgctg cggtttcagg
    26101 atatttaccg aagaagtgtt cacgaacgta cgcaccatct ttcgatttga aggtctggta
    26161 gtcgccgtca acggtttcgt tcatcagctg gatcagttta ccgctggtat ctttacgcag
    26221 cagttcatcc caacggctac cccacatcac tttgataacg ttccagccag caccttcgaa
    26281 gatgccttcc agttcgttga tgatcttgcc gttaccggtg accgggccgt caagacgctg
    26341 caggttacag ttgataacga agaccaggtt atccagtttt tcacgggtag cgatggtgat
    26401 cgcacctttg gattccggtt cgtccatttc accgtcgccg aggaacgcgt aaacggtctg
    26461 tttagaggta tctttcaggc cacggtgttc cagatatttc aggaatttag cctggtaaat
    26521 agcaccaatc ggccccagac ccatagatac ggtcgggaac tgccagaatt ccggcatcag
    26581 tttcgggtgc ggataggaag agaggccatt gccgtgaact tcctgacgga agttatccag
    26641 ctgctcttga gtcagacgac cttccaggaa agcacgagcg tacacgcccg gggagatgtg
    26701 gccctggaag taaaccaggt cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt
    26761 aaagcacaca tcataaatgg ttgcggaaga ctggaaggac gccatatggc cgcccagttc
    26821 gaggtctttt ttcgacgcac gcagcaccgt catgatggcg ttccagcgga tagctgaacg
    26881 aatacggcgt tccagttcca gattacccgg atactccggt tgttcttcaa cggggatggt
    26941 gttgatgtag ttgctgatac ctgtgcctgc ggctacgttt acaccgcctt tgcgggcttc
    27001 agcaagcagt tggtcgatca gatactgagc acgctcaaca ccttcttcac ggatgaccga
    27061 ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc
    27121 tgacatgggt tattccttat ctatctaata acgttgagtt ttctggaacc tgtcccattg
    27181 aactctcgcc ggaaagctca ataagacagg ttctacgttt agttgccgcg cttttatata
    27241 cgcttgattt acaacatctt ctggataatt tttaccagaa aaatcactaa ttctttcgtt
    27301 gctccagacg acgcagagaa cgctcacggc ggctctcttc acgacttctg tcgagcaaaa
    27361 tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg
    27421 ccataatcgc ttcaaatatg cgggtgcggt gactactcac cagcggcagc atctcgcgac
    27481 gcgaatagag caattcgaag ttctggcgga cattctgggc caacatcggc tccatacacc
    27541 ttagcagatg aagcagaacc acattgtggg ccgcttcggt gacggcaatc tgatactgga
    27601 gtacggcgtt tgattccgcg tccagatcgc cagactgctg cgccagctct atggcgtggt
    27661 ggagttcacg gatgcgttcc ttgtcttcat cggtactacg cagcgcggcg taataagcgg
    27721 cgataccttc cagggcgtgt cgtgtttcga gcaagtcata ctgtgattca ggatggtcgg
    27781 agagcagctc caccagcgga tcgctgaagc tttgccatag gctgctctgg acaaaagtgc
    27841 cgccaccctg gcgacgaagc aacaagccct tcgcttcgag acgttgaatc gcctcacgca
    27901 aggagggacg ggagacgtcg aactgttttg ccagttcgcg ttccggtggg agtttttcgc
    27961 ccgggcggag agtgccttcg agaatcaaaa actccagttg ctgctcaatc acatcggaga
    28021 gttttggttg gcggattttg ctgtaggcca tgagttcctg tcttaagcca cttgccgaag
    28081 tcaattggtc ttaccaattt catgtctgtg acgctaaagt aacaaagtat tcaccttatg
    28141 tccatacagg ttttgattga aatcatgaaa ctgtgcacat tttaacaact tgacatatat
    28201 aacgtttcaa aattgtaact atgcacaaat gtagacttta cgtaggaaag ggatttttta
    28261 acgattatta aactataaaa atccgaattg aaccgattca cttaccaatt ttttgatttt
    28321 taagtcaata aaaacgaatt taaattcatt ctgcatattg agaggggttg aggctgagct
    28381 ttacaaacgg tttcttttta agcaactcat cttcaaccat gcataaagcg ggtgcattcg
    28441 ctgccgcata ccattattct tgatctgacg gaagtctttt tgtaacaatt caaacttctt
    28501 tgatgtaaaa aaattaatac aacaaacgga attgcaaact tacacacgca tcactgcgta
    28561 gatcaaaaaa acaatcaccg cacgaggttt catgatggaa ggtcaacagc acggcgagca
    28621 gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg gcgcgatagg
    28681 gacagggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag ggattatcct
    28741 gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctgg gtgaaatggt
    28801 ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact ggggcagttt
    28861 tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg ccatggcaga
    28921 gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccaa cctgggtttc
    28981 tgccgccgta ttctttgtgg tgattaacgc catcaacctg accaacgtta aagtgtttgg
    29041 cgagatggag ttctggtttg ccattatcaa agttattgcg atagtagcga tgatcatctt
    29101 cggcggctgg ctgctgttca gtggcaacgg cggcccgcag gcgagcgtta gcaacctgtg
    29161 ggatcaaggt ggtttcctgc cgcacggctt caccggactg gtgatgatga tggcgattat
    29221 catgttctcg ttcggtggtc tggaactggt ggggatcacc gcagcagaag ctgataaccc
    29281 ggagcaaagt atcccgaaag cgactaacca ggttatctac cgcatcctga ttttctatat
    29341 tggttcgtta gccgttctgc tctcactgat gccgtggacc cgcgttaccg ccgataccag
    29401 tccgtttgtg ctgatcttcc acgagttagg cgataccttt gtggcgaatg cgctgaacat
    29461 cgtggtactg actgcggcgc tctccgtgta caacagctgc gtatattgca acagccgtat
    29521 gctgtttggt ctggcacaac agggtaatgc gccaaaagcg ctggcgtctg tcgataaacg
    29581 cggcgtaccg gttaacacca ttctggtgtc tgcgctggtt acagcattgt gcgtattgat
    29641 taactatctt gctccggaat ccgcatttgg cctgttaatg gcactggtgg tatccgcact
    29701 ggtgatcaac tgggcgatga tcagtctggc gcatatgaag ttccgtcgcg ccaagcagga
    29761 acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact ggatctgcct
    29821 gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg cgatttcggt
    29881 atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta aagagaaaac
    29941 cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccatgcag ggtgagggca
    30001 ataatcttta caccaacgct ccgtaaatcg tcagtagcgc aatcaccacc acgaccacga
    30061 acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt ctccacctta tcgacatgcg
    30121 gttcacgcgc cagagagaac tgcgccagac gcgttaacac ctgatactgc gaagtatgga
    30181 agtcacccag cgaagcaaac caggccggta acgctttctc cccatgaccg atcaaggcat
    30241 ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg gcatcaatgc
    30301 cggactgtaa acgatgatgc ggcgtctggt atcgcgccag ccagtattgc catgcacgca
    30361 aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc aaccagaaca
    30421 gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc tcacgcaaat
    30481 actcacgttc gtcgcagcct gccgggacgc cgtgaatcat ggtgagttcg ccagccatcg
    30541 tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga taatgaagac
    30601 gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc agcgtgggaa
    30661 cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc atcgcaataa
    30721 tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac ccgccgaaag aacgcttcaa
    30781 gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa attaacacca
    30841 gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agcgcacgaa accgtgccca
    30901 atcaaatgca ggaccgggat cggttttccg atccggcgca atatcacaat ggcccgtcat
    30961 gttattagcg atatcgggat agcgatctat cagtgcccgc gtaaccgccg caagctgttg
    31021 atactgcgca tcggtatacg ccagagtatc ggtgccttca agctcaatcc caatagaaaa
    31081 atcattgcag cgttcacgcc cctgatactg agagactccc gcatgccagg cacgtttatc
    31141 gaaaggaaca tactggacta tttcaccatc acggcgaatc aaacagtgag cggagacgcg
    31201 caaatgggcg atctcagcaa agaaaggatg tgcctgcgga tcaatagttc cagtgaataa
    31261 tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt gcaccaccag
    31321 cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa cgcggcgcgc
    31381 gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg gtgctaatac
    31441 ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag ttttaacatg
    31501 ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg cattaatctc
    31561 gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac agtcgatgcc
    31621 aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc cacggtgagc
    31681 acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaggtgtt tattcaactg
    31741 gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat caacgccaat
    31801 caacccttgt tcgaacttga aggcccatcc cgcgtgctgt taacgggcga acgcactgcg
    31861 cttaattttg tgcaaaccct ttcaggagtt gccagtaagg tacgccacta tgtcgaatta
    31921 ctggaaggca ccaacacgca gttgttggat acgcgcaaaa ccttacctgg cctgcgttca
    31981 gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg gctttctgat
    32041 gccttcctga tcaaagaaaa tcatattatt gcctccggct cagtgcgcca ggccgtcgaa
    32101 aaagcgtcct ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa tctggaagaa
    32161 cttgatgaag ccctgaaagc aggagccgat atcatcatgc tggataactt cgaaacagaa
    32221 cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt gtctggcaac
    32281 gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat ctccgtcggt
    32341 gcgctaacta aacacgtaca agcactcgac ctttcaatgc gttttcgcta attgctgaca
    32401 gatttatccg ctccatcgcg ggcggataat ctccccttcc ccaactttct tcgtaacgcc
    32461 tcgcaaattt cttgtctttt gctgcaacga aataaaaaac ctcggaagct gctttcccgt
    32521 ttcgtttttt gcccctcgct tgtgctgatg gcgtttgcca aagtagcgcc aaccaaatca
    32581 aggagcgaaa cagatggaca agcaacgcgg ttttacactt atcgaactga tggtggttat
    32641 tggcatcatt gccattttaa gcgccattgg tattcccgct tatcaaaact acctgcgcaa
    32701 agctgcactc accgacatgc tacaaacctt tgtgccttac cgtacagccg tagagttgtg
    32761 cgcgctggaa catggtggat tagatacctg cgacggtggc agcaatggca ttccctcgcc
    32821 taccaccacc cgctatgttt cagccatgag tgtggcaaaa ggcgtggtgt cgctgaccgg
    32881 gcaagaaagt ctcaatggac taagcgtcgt catgacaccg ggttgggata acgcaaacgg
    32941 cgtcaccggc tgggcgcgca actgcaatat tcaaagtgac agcgcattgc agcaagcctg
    33001 cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg gcaatgaata ttccacagct
    33061 caccgccctg tgtctgcgtt atcagggagt cttgctggat gccagcgaag aggtggttca
    33121 tgttgcggta gtcgatgcac cttcgcatga gttgctggac gcattgcatt tcgctaccac
    33181 caaacgtatt gagatcacct gctggacgcg ccaacaaatg gaaggccacg ccagtcgcac
    33241 acaacagaca ttgcccgtag ctgttcagga gaagcatcag cccaaagcag agttgctgac
    33301 tcgaacgtta caatctgcgc tggaacaacg tgcgtctgat attcatatcg aaccagcgga
    33361 caatgcctac cgcatccgct tgcgtatcga cggcgtattg catcctttac cggatgtttc
    33421 accggatgcc ggagtcgcat taaccgccag attaaaagtg ctgggaaacc tggatattgc
    33481 ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa acgccgtctc
    33541 atttcgtatt gcgaccttac catgtcgggg tggtgaaaag gtggtattaa ggttgttaca
    33601 gcaggtgaac caggcactgg atgttaacac gctgggaatg cagccgttac aactggcgga
    33661 ctttgctcat gccttgcaac aaccacaggg actggtgctg gtaactggcc ctacaggcag
    33721 cggcaaaacg gtcacgcttt atagtgccct gcaaaagctg aataccgctg acattaatat
    33781 ttgtagcgtc gaagatccgg ttgagatccc catagccgga ctaaaccaga cgcaaatcca
    33841 tccgcgtgcc gggctcacct ttcagggcgt tttgcgtgcg ttattgcgcc aggatcctga
    33901 cgtcatcatg atcggagaga tccgctatgg ctttaacggc agaaattgcc attaaagccg
    33961 cgcaaaccgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg
    34021 tacgtttaca gcaaatggga gtcgcccgct ggatgctctc atcagcgctt acgctggtaa
    34081 tagcccagcg tctggtacgt aaactttgcc cacattgtcg ccagcagcaa ggggagccca
    34141 tccatattcc agtcaatgta tggccgtcgc cgctgcccca ctggcaggca cccggttgtg
    34201 tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc
    34261 cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac
    34321 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
    34381 ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg
    34441 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg agcagagagc
    34501 cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga
    34561 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
    34621 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
    34681 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc
    34741 attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg
    34801 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
    34861 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc
    34921 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag
    34981 tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg
    35041 acgctggcag actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg
    35101 gcgatagcca ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg
    35161 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac gcagatcttt
    35221 acgattctgg cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc
    35281 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
    35341 agtaacggtc atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt
    35401 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc
    35461 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
    35521 ccgacgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg
    35581 ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca
    35641 caccagcgtt ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga
    35701 taaatgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg
    35761 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
    35821 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
    35881 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
    35941 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
    36001 cgccaaaggc ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc
    36061 cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga
    36121 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
    36181 cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa
    36241 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
    36301 aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt
    36361 tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat
    36421 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
    36481 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca
    36541 tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
    36601 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
    36661 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
    36721 tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc
    36781 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
    36841 tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat
    36901 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
    36961 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
    37021 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
    37081 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
    37141 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
    37201 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg
    37261 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
    37321 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc
    37381 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
    37441 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
    37501 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
    37561 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
    37621 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
    37681 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
    37741 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
    37801 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
    37861 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
    37921 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
    37981 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
    38041 aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca
    38101 gtctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
    38161 cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt
    38221 ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct
    38281 gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    38341 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    38401 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    38461 aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc gcacgtaggc
    38521 ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga
    38581 acgccttatc cgacctacag acgcttaagc ttcgcaatta ccggttcatt ggctggcgga
    38641 aaatcatcgg cattaagacc gactagcgac atccactcac cgggttgccc ttctttaccc
    38701 cacggctccc cttcccagct ttcgaccagc cagaaccaca gagttatatg cctgtccggg
    38761 aattcatatt ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacttcttcc
    38821 tgaagttcac gcactaccgc ctgttccggc gtttcgccca tttcaatttt accgccggga
    38881 aactccagtt tattcgccat gtgcgcatct gctgcgcgac gcgttataaa gatttcattg
    38941 ttctcgttgc gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa
    39001 aaaaggcgca gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat
    39061 ggcactgctt gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc
    39121 gctcaccggt ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa
    39181 gctgctgcat ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa
    39241 cctcttcagg catacgtacc tggactttgc tcagcgtgct gataacttca tattttaacg
    39301 actccagcat cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt
    39361 tctgtgcata gccacgcagg tggataccct gacgcagata gtccatcgcc gctaggtgct
    39421 ctttccacag agagtcgaga gtttgcagca tgacgccttt ttcgaagtga cgcatcatct
    39481 cagcaccaac cacttcttct ttacgctgat acacttcgat ggactgcgcc agaattcgct
    39541 cacgcagcgt ctcttcatgc agttctggtt ctttatccag ccactcggca attggcaaat
    39601 cgaggtcgaa atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca
    39661 gtgactgtgg cggaatgtag gcatcaatgg tcgctttgaa cacatcttca cgaatgctgt
    39721 tgatggtttc gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc
    39781 gacgctgatc gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac
    39841 ggctttcaac tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg
    39901 cttcgcctgg cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa
    39961 tacgcatcag cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac
    40021 gaccagaacg accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga
    40081 tgatatgcag gccacctgct tccagtaccg catcgtgacg tacctgccag tcggctttaa
    40141 ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac
    40201 caccgagcac aatatctgta ccacgacccg ccatattggt cgcgatagtc accgcagccg
    40261 gataacctgc ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacgt
    40321 tgtgcttaat accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga
    40381 tagtacccac cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg
    40441 cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg
    40501 gacggttggt cggaacaacg acggtatcca gcttgtagat ggagctaaat tcgaaagctt
    40561 cggtatcagc agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga
    40621 aggtgatcga agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca
    40681 cagcctggtg cagaccatcg gaccagcgac ggccctgcat ggtacgaccg gtgtgttcgt
    40741 caacgatgat aacttcacca tctttaacga tgtagtcgac gtcgcgggta aacagcgcat
    40801 gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat gttggccgga gagtacagag
    40861 actccccttc atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac
    40921 gttcggtcag gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga
    40981 aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt
    41041 tatacatttc cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat
    41101 cgatcaggat ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt
    41161 cttcagggct gaacgccata ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac
    41221 cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt
    41281 tgataccgac agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt
    41341 gcgccaggta gtcgttgacg gtaactacgt gcacgccttt accggttagt gcgttcaggt
    41401 aagcaggcag cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc
    41461 gttcgttaag aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc
    41521 gcttacttgc ctcacgtacc acggcgaaag cttccgggat cagattttcc agcacttcgc
    41581 ctttttccag acgtgcacga aactctgcgg ttttcccttt cagttcttcg tcggagagtt
    41641 tttccatctc cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg
    41701 tgcgatcgtt acgactaccg aaaactttag ttaacaattt gattagcata ataaaatctc
    41761 aaacgccccg cgttgcggag ttaataaaat gaagtaaagg tttattgttg ttaggtgagg
    41821 cgttgagggc cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt
    41881 gcttgtgggg taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc
    41941 tgggtcagca gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa
    42001 gactcttcag caacgggcag tgtttgcggt gccattgcga aagaaagatg acggattacc
    42061 gtgcgaatgg catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgtgtg
    42121 ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg
    42181 gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa
    42241 ctcgccgcaa ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc
    42301 cagcgcgtca gtattccact cacgttattg ccatcccgat cgtattcaaa cgtatttagc
    42361 gcacaaatct tatcattaaa gcctgcagtc cacagcacga atgaagataa gttgtgcgga
    42421 aaagtgctta agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa
    42481 aaatgactgg ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac
    42541 agtataaatg tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg
    42601 cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca
    42661 gtttgttatt cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac
    42721 cacacatgaa caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa
    42781 aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc
    42841 tgccgcccag gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg
    42901 tacgcgcacg gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct
    42961 ggttgctgga atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat
    43021 tgtacggctt aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc
    43081 gaacaaattt tttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa
    43141 acggagcggc gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt
    43201 tatcgatgcc caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat
    43261 catgctcgtt gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg
    43321 ggaaatctac cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg
    43381 cagggcgtaa cgtcagagtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa
    43441 cgatacgttt aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa
    43501 ccgaagtgta ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt
    43561 ccagtcaatt cttaatcagc ttgcttacgc aggaatgctg ggatatccag ataatccggc
    43621 tctttcgcag tttgcggcgc attgtcattc acgactttag caaccggctt ctgctcctgg
    43681 gtcagcggag ccatcccatg ctgctggtag cgatccatca ctggctgctg aacctgctta
    43741 ttggtcacca gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt
    43801 acgcgcagct cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg
    43861 tcggaagcaa atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg
    43921 aagcccgccg tgatgttaac cagcacgccg cgcgcgccag acaggtcgat atcttccagc
    43981 agcggagaag agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg
    44041 ccagaaccca tcattgcgta gcccatctca gacattacgg tgcgtacgtc tgcaaagtcc
    44101 acgttcatca aacccggacg agtaatcagt tcagcgatac cttgcacagc gcctttcagt
    44161 acatcgttcg ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc
    44221 agtttgtcgt tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc
    44281 tgctccgcga atgccatacg cttcttgcct tcaaagttga aaggcttagt gacgacagca
    44341 acggtcagga tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg
    44401 gtaccaccac ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc
    44461 aatgcatcgc gatcctcatc agccgcattg cgaccaactt ctggattagc gccagcgccc
    44521 agtcctttgg tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc
    44581 gcttgtgcat cggtatttac cgcgaagaat tcaacacctt caatacgctc gcgcaccatg
    44641 tgttcaacag cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg
    44701 gtaagttcca ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt
    44761 aatcatcgtc ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg
    44821 atccacgagc caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt
    44881 gactctttcc catagtgaag caatcccacc gccgtcgaat aatacggctc ctgagcataa
    44941 tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc
    45001 tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac cggttaatac aatgcctgcc
    45061 gccaggtgat gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg
    45121 ttgaccaggt tgagcagctc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt
    45181 tgcagactcc gtggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca
    45241 acgatggaac ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc
    45301 gtgccaaagg cgtaagcgat atcactggtc acgacattgc cagcataagg aattacctta
    45361 gtgtggcgca atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg
    45421 acgacgcaga cacccagttc acgttcatct tccgtcaata ccgaataact tgatgccagt
    45481 ccggcaaata tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt
    45541 ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg cacgcccgaa
    45601 agtcctaccg gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca
    45661 tgcagcacac gatgttcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc
    45721 acatcttctt gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag
    45781 ctgatgtgct taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat
    45841 tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg
    45901 cctttatcca taccacgtga cgggcagctg cccacgccaa tgatattgac cataccgtcg
    45961 ggcagaactt cccctactaa agcggcaacc ttcgcggtac caatctccag tcctactacc
    46021 agttttctgt ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag
    46081 tagattcctc tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat
    46141 caacgtagct aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt
    46201 ctacaaagcg agccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt
    46261 tattcagcgt caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc
    46321 tgtcctttgc cagcatctgc cccatttcgc gatagccctg caacacttca ttggcgctgc
    46381 cttccgggcc atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga
    46441 aggtgtttcc ttccgcgtct accatatgtt gatcattcca ccgcgcaatc ggcacatatt
    46501 caaccagatg aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc
    46561 acggcaggcg ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac
    46621 ccggctcacc caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt
    46681 caccggtcaa caccagcttt gagagcggca ggcgttgcgc atcttccatc cagcccaaca
    46741 cgacccagcc gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca
    46801 gacgcgttcc attattgcgg cgagaagaaa cctcttcttc gctatttcgc gtgttcagag
    46861 cagcctgcga catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc
    46921 atacctgcct gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta
    46981 ttggcttcca gcagataaaa ctgtccatcg ctgtccagca taacgtcaat acgtccccat
    47041 cctttgcaac ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt
    47101 gacgcttcca gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca
    47161 taatcataga aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt
    47221 atcgcaaccg tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc
    47281 tgaaatgcca atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc
    47341 acactggaac cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct
    47401 gctaactgct tatcgctcag gcctttttca aactctgcgc gggttaacgc tacccacggc
    47461 gcgaccggta aaccggcacc ttgccacagc agtttgctgc gtagtttatc cattgaaagc
    47521 gcagatgcca tcactccgct gccggtataa ggcaagccca tcagctcaag catgccctgc
    47581 agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa acactttctg aaaacccatc
    47641 gacttcagtt gcgtcacgtc gacttctttc gggtcgacag gatacgcatc aataccgcct
    47701 tcacgcagcc cggctaacac cgctgcgcca gaattcagag aaacttcccg ctcagcggaa
    47761 gtcccgccca acaggaccgc aattttatca gtcatgttgt tcttcctccg gagtttgcgg
    47821 cttcagtttg atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac
    47881 gagaatcagg tcgttaccga ttaataccgg tgccagcatc tcggctacct gcgcagggtc
    47941 cggcaccaga atgggatcaa ttttcccacg tccacgaatt gtgcgacaca gcgaacggct
    48001 gtccgctccc ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc
    48061 aacctgcgtc agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg
    48121 cggctgaaac agcattacta ggtttttatc cggccagcct gcgcgcgccg ctttaatggt
    48181 ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt
    48241 accattcact ggctccagcg ggaattcacc gaggaaatca aaacggcgac cagtcccctg
    48301 gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc
    48361 aaccgcagct gcggcgttca gcgcgttatg acggcctggc gcattcaggg tgacgcgcat
    48421 cggctctttg tcctggcgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc
    48481 ttctacacgc acgtcggcat cttcgctgaa gccgtaagtc gtggtctgac gtcccacacg
    48541 cggtaacaat tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaacgg
    48601 caggttgtgc agaaaattaa taaaagtctg ttttaaattc tcaaagtcgc cctggtaggt
    48661 atccatgtgg tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa
    48721 cgatgcatca ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg
    48781 aacccccgcc gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc
    48841 gtagatgctg gaaaccatcg cggtggttgt cgttttgccg tgcgttcctg caatggcgat
    48901 gccatgacga aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg
    48961 cgcttcatga gctgcgacaa tttccgggtt atcggcagaa atggcgctgg aaacaacgac
    49021 cacgctggca tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg ctcccagatt
    49081 cattaactgc tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc
    49141 attggccaga acttcggcaa taccgcccat accggcacca ccgatgccga caaaatgtat
    49201 gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt
    49261 cattctttac gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac
    49321 gcccgggcag cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca
    49381 gcgcgggcgc gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg
    49441 gcgacagcat ccacgctaag ctgtggctgc tcgataattt tggctgcgcc cgctttttcc
    49501 agcggtagcg cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc
    49561 ggtagtcctg ccgcggcgat ttcactcacc gttaaagcac cggagcggca aacgacgaca
    49621 tccgcccacg catacgccgc cgccatatca tcaataaatt ccgtcacttt atgctgcggt
    49681 tgccccgctt cggcatacgc ctgttcaacg gattgttgcg aacctttacc gctctgatgc
    49741 cagatagtga ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg
    49801 cgtgcgccct gggaaccacc cactaccagc acacgaaccg gaccttcacg tccagccaaa
    49861 cgttgctgcg gcaacggcag cgccagcaca tcggtacgca ccgggttacc caccacttcc
    49921 gcattaggga aagcgcctgg aaacgcctgc atcactttgg tggcaatctt cgccagccat
    49981 ttattggtta agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac
    50041 cacgcggcca gaccacctgg acctgataca tagccgccca taccgagtac cacgtcaggt
    50101 ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca
    50161 gctatcagtg cttttatacc ttttccacgc agaccagaga tacgaatgaa atcaatttcg
    50221 atgccatgtt ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact
    50281 tgccaaccct gagccattag atggtgcgca accgccagtc ccgggaatac atgtccaccg
    50341 gttccgcctg ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa
    50401 acgcctgcgc tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg
    50461 tcgacataat cagtaagctc gaaccaccgt aactgatcag cggcaatgtc agacctttgg
    50521 tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc
    50581 caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat ttctaacgct ttgcggccaa
    50641 tcgacatcgc gcgaaaagcg acgaagaata ccattaaaag tgccagcacc acaccgacat
    50701 accccagttc ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact
    50761 ccagtttttg taccgagtta cctaaacctt gtccccaaag ttcgccgcga ccaaacgcca
    50821 tcagcgattg cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg
    50881 cggtaacacg gcggatacgg tacggttcgg cgagtatcag caacacaacc gctgaaatgc
    50941 ccataccgat aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca
    51001 gcgtagtcac aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg
    51061 ccaacaccag aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt
    51121 cgcctttacg caccagatag ttggcgatat aacaaaacag cgacagtttt gtcagctccg
    51181 caggctggat acgcagcaaa ccgagatcga tccaacgcga tgccccttta accgagctac
    51241 ccactaccag cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc
    51301 gttgccagaa ctccatcggc agacgcagcg taatgatcgc cagaatgaat gccaggatca
    51361 gatagacgcc atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg
    51421 gcattgacgc cgaggtcacc atgataaagc caatcgccgc aaggccgaag gtcagccaca
    51481 gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc
    51541 agccttttag cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt
    51601 tcaggcgagg gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt
    51661 gcctcgttgt tcaaagttct tgaactgatc aaggctggca caggccgggg agagcagaac
    51721 catatcgccc agctgaacac gcggagccag caagcgcatc gcttgttcca tggtttcggt
    51781 ttgttctgcc acttccgggc gtagcgccgc cagctgcgca ccgtcacgac caaaacagta
    51841 caaacgcacg ttgtcgccat tcagatagcg cgccagtgga ctgaagtccg ccgatttacc
    51901 atcgccgccc agcaacaaat gcagcgtgcc gtctacctgc aagccattca acgccgcttc
    51961 cgtactgccg acgttagtgg ctttcgaatc gttaatccag cgtacgccat tgtgctccag
    52021 caccacttca aagcgatgcg gcagaccggt gaaggtggtt aacgctttca ggctgctggc
    52081 gcgcggcaaa ccagctgcgt ctgccagtgc cagtgctgcc agcgcattgg tgtagttatg
    52141 ctgcccggaa agtttcatct ctttcacatt cagcactttc tcgcctttaa cccgcagcca
    52201 ggtttcgccc tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca
    52261 gcgttcatca gcaccgcgaa tcggcattgt taaggcatca tcagcattga cgacacaaac
    52321 tttcgcattt tcgtaaatac gcaattttgc cgcacggtat tgctgcaaac caaacggata
    52381 gcggtccata tgatcttcag tcacgttcag aatggtcgcc gccaccgcct gtaagctgga
    52441 agtggtttcc agctggaagc tcgacagttc cagcacgtac agttcgcact cagcatcaag
    52501 cagcatcaac gctggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt
    52561 cgccatttca cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac
    52621 aatcggtgct tgtgcttcgc gacagaacag ctcgatatcg ccaacgattt cgattccggc
    52681 atcagcggca gcgcttaagg atggatgcgc ctgtgcaata ccgggactgg cgacaatcag
    52741 atctgccgcc atcagccatt catcattcag actgcccgtg tggcgttcta cggcttcggg
    52801 taatttatcc aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg
    52861 agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacgacatt
    52921 tttaccctga taatcagcca tgattaacgt accttcagcg ttgccagacc aatcagaacc
    52981 agcatcagcg aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt
    53041 tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag
    53101 ccgacctgca ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc
    53161 aggaattcct gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa
    53221 cctacatcgc ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct
    53281 gccccgacta tcgccgtgca gacaatgacc agttcccctg cgtgtcgcag atacggtata
    53341 tgcaagtagc tggcaaagtt catattgccg gtcgcccacg ccaccagcgc aaaaccaccg
    53401 gcgacaaata cggtcggcat aattgccagg ccgtcgagac catcggtcag gtttaccgcg
    53461 ttgccagtac ccacaatgac gaagtaagcc agcagaatgt agaacagccc cagctgcggc
    53521 atcacatctt taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg
    53581 tacagggcga aggcgacacc cagcgcaatg accgacatcc agaaatactt ccaacgagcg
    53641 atcaaccctt tggtgtcttt acgcaccact ttgcgataat catcaacaaa gccaataaca
    53701 ccgtaaccta ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac
    53761 agcagtacgg agatcacaat cgccgtcagg atcataatcc cgcccatggt cggcgtaccg
    53821 cgcttgctga agtgtgattc aggaccgtcg ttacgcacca cctgaccaaa ggaaagtttt
    53881 tgcaaatgag ctatcatacg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg
    53941 ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc
    54001 aaatgttcgg ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt
    54061 ccatggcggc actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca
    54121 gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg
    54181 cggtgctgat agcatggctt tgtttaccca cgcttaacac gcggtcaata ccagcagctt
    54241 ttgccgcctc gcccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat
    54301 cgcccaccac cagcacgcgg tagcccggca tttcagccag tacctgaaca gcggcggtca
    54361 ttgaaccgac attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga
    54421 tggggaacag acggcctgga acagctttca gatttgccag ccccgctttg atagcatcaa
    54481 gcgttgcgcc cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac
    54541 gccccggcaa cggcagcaga acatcgacgc taccggttgg ggtttgtagg gtaaattccg
    54601 taccgtgcga ggtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattgggtg
    54661 agaaacgcca cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt
    54721 cggcgttcat aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt
    54781 tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca
    54841 gcgcagcttc cgggcgagtc agactcacag tccaggctat ttcgccctga tggttcgcgc
    54901 caagttcaat aactgcgtaa tcgtattccg gcgtcaagcg caacaacgtc atcggtacac
    54961 cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg
    55021 ccgccgtcat ctctttaacg gaggttttgc cggaggaacc tgtcagagca accacgcgcg
    55081 ccggaacttg ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga
    55141 cgattaactg cggcaggtcg atatccagcg gacggctgac cagtagtgcg cccgcgccgc
    55201 cagctttcgc ctggtcggca aaatcatgag catcgaaacg ttcgcctttc agggcaacaa
    55261 acaggcagcc cggcgtcagt tttcgggtat cagtggttac agcatcaagg gtgatatctg
    55321 caccttgcag ttcaccgttg agaatgtcgg taagttggct aagggttacg ctaatcatgc
    55381 aatcaccccc agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc
    55441 aacaatctgg taatcttcat ggcctttgcc cgcgaccagt accacatcat tctctttagc
    55501 ctgcataacg gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc
    55561 atctaacatt cccgccagaa tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt
    55621 atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca attgcgccca tcagtggacg
    55681 cttaccttta tcgcgatcgc caccacagcc aaagacgcac cacagcttgc ccgcacagtg
    55741 caggcgcgcc gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac
    55801 caccgtcggt ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg
    55861 cgcggcggtt ttcagcagat cagccagtgg atagccgagt gccagcagtg tcgccagcgc
    55921 gagcagcagg ttgctgacgt taaaagcgcc catcagatgg ctttcaattt cgccatcgcc
    55981 ccaacttgag ctaaagcgaa tcgtcgcacc gctgtcgtga tagttcacgt cgatcgcttt
    56041 taaccagcgt ccgtgacagt tcggattaat atgatcttcc attgataccg caaccgcgtc
    56101 cggcagtttt gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc
    56161 gcaatgatgt tcagaataaa gcagccattt cgcagcttcg tagtgttcca tatcaccatg
    56221 ataatcaagg tgatcgcggc ttaagttggt aaagaccgac gccgcaaatt tcaatgccgc
    56281 cacacggtgc tgtaccagcc cgtgggagga aacttccatt gcgcaaaacg tcgcgccctg
    56341 ctccaccagc cccgccagct catgctgaac atcgactgcg gaaccggtag tattttctgt
    56401 cgggatcact ttcccaagca gaccgttacc aacggtgccc attaccgcgc tggtttcgcc
    56461 aagcagttgg ctccactgcg ccagcagctg ggtagtcgtg gttttgccgt tggtgcccgt
    56521 tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga
    56581 taaacgctca ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc
    56641 accgtcggtc gcctcatctt tcgctttcgc ctctgcaata atggcagcca caccttgcgc
    56701 tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag
    56761 atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc
    56821 gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc
    56881 cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca
    56941 tggtacgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg gcgccgccgt
    57001 agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg
    57061 caggcgcaac gcctgcggta taagcaatat atttattgat gtagcgaccg tccggcccga
    57121 cctttttcgc ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc gccttcacgc
    57181 cgccgccgcc tggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatggatc
    57241 ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat
    57301 aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta
    57361 gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat
    57421 ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg
    57481 agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt
    57541 tactcgactt ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga
    57601 tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc
    57661 cacgttgcaa cgcggtcatt accaccatcg gtttaaccgt tgagcccggt tcaaacacgt
    57721 cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt
    57781 atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc
    57841 taccagattc agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct
    57901 gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa
    57961 tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg
    58021 taagccattt atcgaaactc ttctcaacgc cctcaatccc ttgactatcg acgttagtaa
    58081 agccgatgag gtgagcagtc acttcgccgg acggatagta acggcgagac tcttcacgca
    58141 gatgaatccc cggcagtttc agttttttga tgtagtccgc catgtcaggg ttcacctgac
    58201 gcgccagata aataaaacgc cctttcgggt tggcgttaat gcgggctgaa agctgatcca
    58261 gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag
    58321 cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg
    58381 ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag
    58441 aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc
    58501 gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac
    58561 tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca
    58621 tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct
    58681 gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct
    58741 cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt
    58801 cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacagtc accgccgtca
    58861 aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga
    58921 taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag
    58981 cttctgtcac tctgctgatc atgcattcgt cctctctgca atacgcagaa ctgaactacg
    59041 ggcacgaggg ttctcagcca cctcttcttc gcccggcatt aacttgccta gtgctcgcag
    59101 ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg
    59161 cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa
    59221 gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg
    59281 ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc
    59341 gggatgttta aacttatctt tcaccggcgt tgcaacagcc acgacttccg ccagttcttt
    59401 ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc
    59461 aaaacgctct tcaccatagg ttttcaatac ccaagcgata tcggcttctt ctgcggtttg
    59521 tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc
    59581 gcgcataaag gaaaagccgc gctcagcatc atcaagttgc ggtgaagaga cgccaagatc
    59641 gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc
    59701 ggagaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat
    59761 agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat
    59821 cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat
    59881 attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat
    59941 tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt
    60001 cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga
    60061 actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga
    60121 ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct
    60181 gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt
    60241 cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca
    60301 tttgaccgac agcgttctca agcagctgtt cccggtaacg ggtaggcact gataagcgcc
    60361 ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc
    60421 ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca
    60481 agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg
    60541 tttattttac gtccaacgtg cgcagcaaat taacgttatg aatagctgta agaaaaaatc
    60601 cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc
    60661 agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg
    60721 tctgtgtggc gctt
//
LOCUS       DAJTVE010000007        58140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000007 DAJTVE010000000
VERSION     DAJTVE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..150
                     /locus_tag="OO439_000427"
     CDS             <1..150
                     /locus_tag="OO439_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6314891.1"
                     /translation="AHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAIY
                     LRQES"
     gene            complement(188..1249)
                     /locus_tag="OO439_000428"
     CDS             complement(188..1249)
                     /locus_tag="OO439_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6314892.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1462..2925
                     /gene="bepA"
                     /locus_tag="OO439_000429"
     CDS             1462..2925
                     /gene="bepA"
                     /locus_tag="OO439_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6314893.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2946..3305
                     /gene="arsC"
                     /locus_tag="OO439_000430"
     CDS             2946..3305
                     /gene="arsC"
                     /locus_tag="OO439_000430"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6314894.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3443..4189)
                     /gene="hda"
                     /locus_tag="OO439_000431"
     CDS             complement(3443..4189)
                     /gene="hda"
                     /locus_tag="OO439_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6314895.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4239..5528)
                     /gene="uraA"
                     /locus_tag="OO439_000432"
     CDS             complement(4239..5528)
                     /gene="uraA"
                     /locus_tag="OO439_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6314896.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5614..6240)
                     /gene="upp"
                     /locus_tag="OO439_000433"
     CDS             complement(5614..6240)
                     /gene="upp"
                     /locus_tag="OO439_000433"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6314897.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6565..7602
                     /gene="purM"
                     /locus_tag="OO439_000434"
     CDS             6565..7602
                     /gene="purM"
                     /locus_tag="OO439_000434"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6314898.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7602..8240
                     /gene="purN"
                     /locus_tag="OO439_000435"
     CDS             7602..8240
                     /gene="purN"
                     /locus_tag="OO439_000435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6314899.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8411..10477
                     /gene="ppk1"
                     /locus_tag="OO439_000436"
     CDS             8411..10477
                     /gene="ppk1"
                     /locus_tag="OO439_000436"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6314900.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10482..12023
                     /gene="ppx"
                     /locus_tag="OO439_000437"
     CDS             10482..12023
                     /gene="ppx"
                     /locus_tag="OO439_000437"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6314901.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12062..14305)
                     /gene="pdeF"
                     /locus_tag="OO439_000438"
     CDS             complement(12062..14305)
                     /gene="pdeF"
                     /locus_tag="OO439_000438"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6314902.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14487..14639)
                     /locus_tag="OO439_000439"
     CDS             complement(14487..14639)
                     /locus_tag="OO439_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6314903.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14657..14848
                     /locus_tag="OO439_000440"
     CDS             14657..14848
                     /locus_tag="OO439_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6314904.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15159..15677
                     /locus_tag="OO439_000441"
     CDS             15159..15677
                     /locus_tag="OO439_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6314905.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15693..16232
                     /locus_tag="OO439_000442"
     CDS             15693..16232
                     /locus_tag="OO439_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6314906.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16327..17904)
                     /gene="guaA"
                     /locus_tag="OO439_000443"
     CDS             complement(16327..17904)
                     /gene="guaA"
                     /locus_tag="OO439_000443"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6314907.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17973..19439)
                     /gene="guaB"
                     /locus_tag="OO439_000444"
     CDS             complement(17973..19439)
                     /gene="guaB"
                     /locus_tag="OO439_000444"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6314908.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19601..20971
                     /gene="xseA"
                     /locus_tag="OO439_000445"
     CDS             19601..20971
                     /gene="xseA"
                     /locus_tag="OO439_000445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6314909.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20968..21183)
                     /locus_tag="OO439_000446"
     CDS             complement(20968..21183)
                     /locus_tag="OO439_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6314910.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21252..22724)
                     /gene="der"
                     /locus_tag="OO439_000447"
     CDS             complement(21252..22724)
                     /gene="der"
                     /locus_tag="OO439_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6314911.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22842..24020)
                     /gene="bamB"
                     /locus_tag="OO439_000448"
     CDS             complement(22842..24020)
                     /gene="bamB"
                     /locus_tag="OO439_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6314912.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24031..24651)
                     /gene="yfgM"
                     /locus_tag="OO439_000449"
     CDS             complement(24031..24651)
                     /gene="yfgM"
                     /locus_tag="OO439_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6314913.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24669..25943)
                     /gene="hisS"
                     /locus_tag="OO439_000450"
     CDS             complement(24669..25943)
                     /gene="hisS"
                     /locus_tag="OO439_000450"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6314914.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26054..27172)
                     /gene="ispG"
                     /locus_tag="OO439_000451"
                     /gene_synonym="gcpE"
     CDS             complement(26054..27172)
                     /gene="ispG"
                     /locus_tag="OO439_000451"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6314915.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27199..28212)
                     /gene="rodZ"
                     /locus_tag="OO439_000452"
     CDS             complement(27199..28212)
                     /gene="rodZ"
                     /locus_tag="OO439_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6314916.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28497..29651)
                     /gene="trmG/rlmN"
                     /locus_tag="OO439_000453"
     CDS             complement(28497..29651)
                     /gene="trmG/rlmN"
                     /locus_tag="OO439_000453"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6314917.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29801..30232)
                     /gene="ndk"
                     /locus_tag="OO439_000454"
     CDS             complement(29801..30232)
                     /gene="ndk"
                     /locus_tag="OO439_000454"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6314918.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30381..32693)
                     /gene="pbpC"
                     /locus_tag="OO439_000455"
     CDS             complement(30381..32693)
                     /gene="pbpC"
                     /locus_tag="OO439_000455"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6314919.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32694..37655)
                     /gene="yfhM"
                     /locus_tag="OO439_000456"
     CDS             complement(32694..37655)
                     /gene="yfhM"
                     /locus_tag="OO439_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6314920.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37862..38707
                     /gene="sseA"
                     /locus_tag="OO439_000457"
     CDS             37862..38707
                     /gene="sseA"
                     /locus_tag="OO439_000457"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6314921.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39200..39976)
                     /gene="sseB"
                     /locus_tag="OO439_000458"
     CDS             complement(39200..39976)
                     /gene="sseB"
                     /locus_tag="OO439_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6314922.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40118..41401)
                     /gene="pepB"
                     /locus_tag="OO439_000459"
     CDS             complement(40118..41401)
                     /gene="pepB"
                     /locus_tag="OO439_000459"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6314923.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41579..41779)
                     /gene="iscX"
                     /locus_tag="OO439_000460"
     CDS             complement(41579..41779)
                     /gene="iscX"
                     /locus_tag="OO439_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6314924.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41791..42126)
                     /gene="fdx"
                     /locus_tag="OO439_000461"
     CDS             complement(41791..42126)
                     /gene="fdx"
                     /locus_tag="OO439_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6314925.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42128..43978)
                     /gene="hscA"
                     /locus_tag="OO439_000462"
     CDS             complement(42128..43978)
                     /gene="hscA"
                     /locus_tag="OO439_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6314926.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(43995..44510)
                     /gene="hscB"
                     /locus_tag="OO439_000463"
     CDS             complement(43995..44510)
                     /gene="hscB"
                     /locus_tag="OO439_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6314927.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44606..44929)
                     /gene="iscA"
                     /locus_tag="OO439_000464"
     CDS             complement(44606..44929)
                     /gene="iscA"
                     /locus_tag="OO439_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6314928.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44946..45332)
                     /gene="iscU"
                     /locus_tag="OO439_000465"
     CDS             complement(44946..45332)
                     /gene="iscU"
                     /locus_tag="OO439_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6314929.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45360..46574)
                     /gene="iscS"
                     /locus_tag="OO439_000466"
     CDS             complement(45360..46574)
                     /gene="iscS"
                     /locus_tag="OO439_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6314930.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46815..47303)
                     /gene="iscR"
                     /locus_tag="OO439_000467"
     CDS             complement(46815..47303)
                     /gene="iscR"
                     /locus_tag="OO439_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6314931.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47604..48341)
                     /gene="trmJ"
                     /locus_tag="OO439_000468"
     CDS             complement(47604..48341)
                     /gene="trmJ"
                     /locus_tag="OO439_000468"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6314932.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48460..49263
                     /gene="suhB"
                     /locus_tag="OO439_000469"
     CDS             48460..49263
                     /gene="suhB"
                     /locus_tag="OO439_000469"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6314933.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49408..50262
                     /locus_tag="OO439_000470"
     CDS             49408..50262
                     /locus_tag="OO439_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6314934.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50453..51733
                     /gene="csiE"
                     /locus_tag="OO439_000471"
     CDS             50453..51733
                     /gene="csiE"
                     /locus_tag="OO439_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6314935.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51725..52864)
                     /locus_tag="OO439_000472"
     CDS             complement(51725..52864)
                     /locus_tag="OO439_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6314936.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53024..53914)
                     /gene="hcaR"
                     /locus_tag="OO439_000473"
                     /pseudo
     CDS             complement(53024..53914)
                     /gene="hcaR"
                     /locus_tag="OO439_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54050..55411
                     /locus_tag="OO439_000474"
     CDS             54050..55411
                     /locus_tag="OO439_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6314937.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55408..55926
                     /locus_tag="OO439_000475"
     CDS             55408..55926
                     /locus_tag="OO439_000475"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6314938.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55926..56246
                     /locus_tag="OO439_000476"
     CDS             55926..56246
                     /locus_tag="OO439_000476"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6314939.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56243..57055
                     /gene="hcaB"
                     /locus_tag="OO439_000477"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56243..57055
                     /gene="hcaB"
                     /locus_tag="OO439_000477"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6314940.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57065..>58140
                     /locus_tag="OO439_000478"
     CDS             57065..>58140
                     /locus_tag="OO439_000478"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6314941.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGV"
BASE COUNT        14201 a        15734 c        14500 g        13705 t
ORIGIN      
        1 gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat taccgccaat
       61 ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag tatgtgttat
      121 cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca gttcgtcggg
      181 ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg gctttgatca
      241 gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca ccgaagatca
      301 ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac aacaccggta
      361 ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag ctccagaatt
      421 ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc accacaaatg
      481 cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc agcgaatagt
      541 tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc atctccagca
      601 ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc tgtcctgcca
      661 gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct ttgtctttca
      721 gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc agcaaaccga
      781 ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc cgactgcgca
      841 tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag cggcgcggca
      901 acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg atcaagtaga
      961 tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat ataccgacga
     1021 aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat tgcaggcgca
     1081 cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga gcaagcaggc
     1141 cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag gcaatcgctt
     1201 ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata aagaccttcc
     1261 ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca aaaaagactt
     1321 cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa tcttcgccgt
     1381 tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa tgactatctt
     1441 cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt tgcaaccctc
     1501 attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc agacaccttg
     1561 ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat gcagatgggc
     1621 gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc gctgttaacg
     1681 caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt taagacaccg
     1741 tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt tggcggcaac
     1801 gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact ggcttcagtt
     1861 atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat ggaagatcag
     1921 cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact ggcgatggcc
     1981 agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg tcaggggatg
     2041 atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca ggtgctgcaa
     2101 cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact cgatcaggcg
     2161 cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga aagtcgtctg
     2221 gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc gtcggaagat
     2281 ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa ccagctcacc
     2341 agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc ggcgcaatat
     2401 ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa aacgctgcaa
     2461 ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac cgatatcgat
     2521 cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg tgatttgcgc
     2581 accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg tcaaccacaa
     2641 gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag caacggctgg
     2701 gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct ggctgcgcga
     2761 gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt gagtagcgcc
     2821 agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg catcgaccag
     2881 ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc attaaaggaa
     2941 gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag agccgggaaa
     3001 cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac cttgagacac
     3061 ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat agcgcccgtg
     3121 aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac agttcgctta
     3181 gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag cgcccgattg
     3241 tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg gaaatcgtgg
     3301 gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct tgccatgtct
     3361 tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc gttcgcgccg
     3421 catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac ggaatggtca
     3481 gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata aatagcgtgc
     3541 gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc agttcaaaac
     3601 cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa agtggctgca
     3661 atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc agattcaact
     3721 gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc agaattcgat
     3781 tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac tcaatgttgt
     3841 cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca aaccaggtgc
     3901 gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc gcgcaagccg
     3961 cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg taaccgctat
     4021 gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc cccggccaga
     4081 aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt gccggtgtgt
     4141 tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca gaatcccatg
     4201 atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt tatatccgcg
     4261 tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt gaagatcaaa
     4321 cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc cgcgccaatg
     4381 tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat caggttctgt
     4441 gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc accgatgaca
     4501 ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg gatagcggca
     4561 gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc gataacccag
     4621 gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt ggtatttggc
     4681 gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat cgaacggtgc
     4741 agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac caggtgccct
     4801 acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc aaaccactcg
     4861 aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat cggcgtggta
     4921 tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat taaaatcggg
     4981 ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc cagggtggta
     5041 atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag taaacccgcc
     5101 ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc gcccattgcc
     5161 gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat aaaagaaacc
     5221 aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata ccctaacggc
     5281 aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc cggaattttc
     5341 cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag tacagtcgcc
     5401 gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc gaacaaatgt
     5461 tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc gatagcacgg
     5521 cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa aaaaaagccg
     5581 actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga ttttgtcacc
     5641 ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt caatcgatgc
     5701 ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt ctggcgcagc
     5761 taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt cgatggtcgc
     5821 gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac gctcatcgat
     5881 gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat tacggtacat
     5941 accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca tcatccccag
     6001 accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt cgatttctac
     6061 cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg tcgcttcgta
     6121 agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc tgatatcttg
     6181 ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca cgatcttcat
     6241 actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc tcctttcttc
     6301 aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg agataaagtt
     6361 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa cctttgccct
     6421 ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg ttgtgcaaag
     6481 cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
     6541 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
     6601 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
     6661 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
     6721 caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
     6781 ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
     6841 aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta cgcaaccgga
     6901 aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg atgtctgcaa
     6961 tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta tcacggtgag
     7021 gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat catcgacggc
     7081 tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac
     7141 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
     7201 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
     7261 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
     7321 ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat tgatgaatct
     7381 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt tgagcgccat
     7441 gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa
     7501 gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc
     7561 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
     7621 tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta
     7681 aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
     7741 gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt cgtgaagcct
     7801 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
     7861 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
     7921 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
     7981 atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
     8041 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
     8101 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
     8161 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg
     8221 gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg ctggcgtttt
     8281 tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct ggcatagttg
     8341 gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat aaaacggagt
     8401 aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt ggttatcgtt
     8461 caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg aaaggatgcg
     8521 tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct tcgctgaact
     8581 gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc gccatttact
     8641 gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga
     8701 gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc agctctccgt
     8761 caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc acattacgcc
     8821 gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt acacctatct
     8881 ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga tcccatcaga
     8941 taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca agccgatgat
     9001 tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct tctttgatta
     9061 tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg atttagtgca
     9121 tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc gtttaactgc
     9181 tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg aagtgttacg
     9241 cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt atcataattt
     9301 taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc
     9361 gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg atgccattcg
     9421 cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc tggaactgct
     9481 gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt accgtgtggc
     9541 aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca agaaagtgac
     9601 cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact gggcgaagcg
     9661 cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa
     9721 actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac acatcgggac
     9781 cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc tgaccgcaga
     9841 tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc cataccgccc
     9901 ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt tatatgaaat
     9961 ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca ccctgaagct
    10021 aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca gctccggcgt
    10081 accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg aaggcattag
    10141 cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc gggtttatat
    10201 ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa
    10261 tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga agcagcgggt
    10321 gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata tcgataaaga
    10381 actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat
    10441 ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata cacgataaat
    10501 cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt cacatggtca
    10561 tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag cgggtgcatc
    10621 tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg cgcggtttga
    10681 actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc gtctgtatag
    10741 tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa cgcgcggaaa
    10801 aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt ctgattttta
    10861 tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat attggcggcg
    10921 gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa agccgccgga
    10981 tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat aaagagaatt
    11041 ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg caattccgta
    11101 ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc catgaagtgt
    11161 taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa aaactggtaa
    11221 aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt tccgaagagc
    11281 ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat gctttagcca
    11341 tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac gaaatggaag
    11401 gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc
    11461 acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg tacgaacagt
    11521 ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg cgatgggccg
    11581 ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc cactccgctt
    11641 atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg atgatggcga
    11701 cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc tttaccttgt
    11761 ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta ttactcaaca
    11821 atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat gacagtcact
    11881 ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg cttgatctgg
    11941 aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa
    12001 gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg gcccgcatta
    12061 ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac ggctgcggct
    12121 taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc tcgcggatct
    12181 cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc agatggcaaa
    12241 tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata aagctgccgt
    12301 caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg tagccggtgc
    12361 cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag gtgatttgcg
    12421 cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt tgccacgctt
    12481 caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc tgacataccg
    12541 aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca
    12601 taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt
    12661 gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct ttcatgcgca
    12721 gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa
    12781 aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt
    12841 cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg gtcacgatgg
    12901 aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc actggcgagc
    12961 gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc caaaagaaac
    13021 gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca
    13081 gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt tccagcaagg
    13141 gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg ccatagttct
    13201 taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag ggagcatcac
    13261 gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc atatacgcaa
    13321 gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc atataattga
    13381 caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg gtatagccag
    13441 gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg accgcccaga
    13501 gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg ggcagcaata
    13561 atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc atgcataaca
    13621 gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg gtgactttgg
    13681 catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga tttcgcacta
    13741 cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt aaggcctgat
    13801 aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct ctgctcgcca
    13861 gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca ggaaacacaa
    13921 tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg ttgttacgcc
    13981 ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata aaattagccg
    14041 ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa atccctaagg
    14101 cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc agtgccagag
    14161 gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta atgtgggcga
    14221 aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta tcacgcattt
    14281 ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat cctgaaggag
    14341 gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca ctatagagag
    14401 cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa aataaccctg
    14461 attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc attgtaaata
    14521 attaaattaa cttccataac attaaaatat gtatccactg acgttttttt acataacgaa
    14581 gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc gtgggacata
    14641 cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc gatttaacag
    14701 tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc gttttatcta
    14761 ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc aatccacact
    14821 ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac agaacataac
    14881 ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag atattctttc
    14941 atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg cggaataaat
    15001 ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct gcgtgaattt
    15061 taataaattt aatctatccc tttatacgca atacatttac tttcctcttt tgatgatctt
    15121 aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt aaaaaatgtc
    15181 ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca aatgctgacg
    15241 atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa actaaaaccg
    15301 ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa aataaacgga
    15361 acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc ggccacaacg
    15421 tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga gctgtaggcg
    15481 cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct ttaacatata
    15541 aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag tttacgacag
    15601 gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca aatactaaat
    15661 gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt tgcgccatct
    15721 tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc tctgctgtcg
    15781 cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa tgggtcgcgg
    15841 agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag gccgccgcta
    15901 acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa ctggacgccg
    15961 aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc agcttagaga
    16021 ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga gaaaacgaat
    16081 ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa tctgaagctg
    16141 acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg ggcaaagcag
    16201 aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca agcctatgat
    16261 tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat agtgcaggga
    16321 ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat acaccacgcg
    16381 ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga aatcgtacgg
    16441 cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca gagagacaac
    16501 ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca ggaacacagt
    16561 gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa tgaagatggc
    16621 gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca cacgaacgcc
    16681 aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc ccagctccag
    16741 accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca ggcccatctt
    16801 catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg ctttaccggt
    16861 tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca gccacttcac
    16921 gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac cgatgatttt
    16981 acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat cttctgccgg
    17041 aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct gctctgcttc
    17101 gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac cgatagcgcg
    17161 gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga ggatgacttt
    17221 atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt tcgctggcgt
    17281 ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc gcataccctg
    17341 gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt cttcgttagc
    17401 cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg acgggatagc
    17461 ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt ctgcggtcag
    17521 cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct gcgcgtagcc
    17581 aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca tggtctgcat
    17641 gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg gcgcacgcgg
    17701 gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc ttggattgaa
    17761 gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa cacccagctc
    17821 acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa tgaggatgcg
    17881 atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa cagataatat
    17941 aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta gttcggggac
    18001 tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc gctgatacgt
    18061 acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag acccatacag
    18121 gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt ataagctacg
    18181 cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg gaaataacgg
    18241 tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga tttgtaagaa
    18301 cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag catggaacct
    18361 accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga gaagcgaata
    18421 ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc gtcagcaacg
    18481 gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat agaaccaggg
    18541 ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc acctgcagct
    18601 gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt ttcacggata
    18661 cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac gtcaacgcca
    18721 gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac tgcagcacca
    18781 acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc tttctggaag
    18841 tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag cgctttttca
    18901 acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc acgcacggtg
    18961 accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc ggtaacaaaa
    19021 cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac gaccggatag
    19081 cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt ggttggcaga
    19141 acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg aacttcttct
    19201 gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc ctgagccaga
    19261 gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat agggatattc
    19321 agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag aacggtagaa
    19381 tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat acgtagcatg
    19441 ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca gagcgtaacc
    19501 gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg ggttactatc
    19561 gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt ctcaatcccc
    19621 tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg agcatgagat
    19681 gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt ccggtcactg
    19741 gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc gcaacagcaa
    19801 ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg ccaatattac
    19861 gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc cggccggtga
    19921 agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg aaggtttgtt
    19981 cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg tgatcacctc
    20041 aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg atccttctct
    20101 accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc aaattgttcg
    20161 cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg ggcgcggcgg
    20221 cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg cgatttttgc
    20281 cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca ttgccgattt
    20341 tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga gccgtaatca
    20401 gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg cgatggatta
    20461 ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac agcaacagca
    20521 tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa agcggatgag
    20581 ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt taacacagcg
    20641 gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca ttcagcaact
    20701 ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg aacgtttcgg
    20761 taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc gtggatacag
    20821 cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta aagcgggtga
    20881 aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa acatccagcc
    20941 agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc gttttttcga
    21001 tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt ttaacacctg
    21061 caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc cgcaggatgg
    21121 gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac aatgcagttc
    21181 catatgaatt cctccggtag ccatccctct acattcatag agggaatggc agataaaata
    21241 cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg tttacgcatc
    21301 tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc tttgaactga
    21361 atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat caagtagcgc
    21421 ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat cggcgggtta
    21481 taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag cggcggttgg
    21541 tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac acgacgggtg
    21601 gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact gccgtgcaag
    21661 gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc cagcgtttct
    21721 ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac aatgacaagt
    21781 gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga aataccttcg
    21841 cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa cgttttgatt
    21901 acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc agcggtgtca
    21961 atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc acgcgtcgtg
    22021 ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt gagtgtagac
    22081 ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg cgggtcgaag
    22141 tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca gtattcagcg
    22201 tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag cacacgctcc
    22261 agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc acctaaacca
    22321 agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt gtttgccacc
    22381 aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc tgccggcatc
    22441 aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat cgccagcagc
    22501 gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc gccggtatca
    22561 atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc acgagtcaga
    22621 cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa taacgtggat
    22681 tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa agcctcattt
    22741 ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca ggaaaacggc
    22801 ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt aatagagtac
    22861 acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg ttcagtctgg
    22921 aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac gttaatccag
    22981 tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac cggcgaagtc
    23041 agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc aatggtcaac
    23101 gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac gatgaaatca
    23161 ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc aagcgccgtc
    23221 aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg agtcgtgtca
    23281 acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg ctgctgccaa
    23341 atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc ccccacgacg
    23401 gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga aggcatatcg
    23461 aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa ctgaccgtta
    23521 ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc ttcacccgcg
    23581 actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac ctgcgccttt
    23641 tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag taatgcaggc
    23701 tctttagaga accagccatc tttctcggcc aggctgacag accagatttc tttgccatca
    23761 tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac gttgtccgcc
    23821 agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga agtgctccac
    23881 gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt taccacatct
    23941 tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag tcctggcagc
    24001 agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa ttttcatctg
    24061 catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc atgcactacg
    24121 cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg caacaatggc
    24181 agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag cctgcttgag
    24241 ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt catcactcgt
    24301 gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt tgtcaacaaa
    24361 ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt cagcagcaaa
    24421 tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa ccgcattttg
    24481 ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt agcgccagcc
    24541 aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat tttcagcaaa
    24601 aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca cgctgtcctt
    24661 ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg cgcaactgcc
    24721 gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac ttcagactca
    24781 cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg tttcttaaag
    24841 ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg cagacgctca
    24901 gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta tatatcgaca
    24961 acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag acgttcgagg
    25021 cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac cagaccgtca
    25081 taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac ccactcgaaa
    25141 acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt gtaagcgatc
    25201 cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc ctcgtccaga
    25261 taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt ttttgaatcc
    25321 agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag cttttcttta
    25381 tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc cagcgaacca
    25441 atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca ccagcgggca
    25501 gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac ttcgcagccc
    25561 aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa catcggcccg
    25621 atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc ggcgcgtaca
    25681 cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc ctcaaaggtg
    25741 tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa tagcggggtc
    25801 tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac gtttttcagt
    25861 gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt catgccgcga
    25921 atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat gaaccctcaa
    25981 cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa gcgaggcgct
    26041 tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct tcgtccagct
    26101 gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg ttgtccagac
    26161 ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg gtgacgccga
    26221 gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc gaaacgtcca
    26281 tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg ataacatcaa
    26341 attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa cggatacgca
    26401 gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc gccagcgata
    26461 cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg gcggatttta
    26521 ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc tgatcgatct
    26581 gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct ttcacgctga
    26641 ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg gcggattcca
    26701 gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc agcgatccgg
    26761 cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc accatgcgaa
    26821 tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg ccgtattccg
    26881 ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc acgttaaccc
    26941 gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag acacggacga
    27001 tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg acatctgtcg
    27061 tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc ggcacattcc
    27121 caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc atgaaaaatc
    27181 tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg tcagacgcgc
    27241 aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat actggatctg
    27301 tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta aattcaaatt
    27361 accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag tgacctccag
    27421 ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg gcgtggtcac
    27481 gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg ccgttggtac
    27541 cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat tctgttgcgg
    27601 atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg cggtagtatc
    27661 cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg tatttaacgg
    27721 gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat cggccatagt
    27781 ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc tcaggccgat
    27841 aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt ttttgcggcg
    27901 tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg gagcctgctt
    27961 ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat aagagcggat
    28021 atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt caatgtcgcg
    28081 taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa gtcctagttg
    28141 ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt cgtgcgtggc
    28201 ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg tgaaccatca
    28261 tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt ataagaccgt
    28321 ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc atcgttacat
    28381 actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg caacttaata
    28441 cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa tggctatcag
    28501 accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg tttcgtacgg
    28561 tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc acgagtttta
    28621 cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc gatacggctg
    28681 ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag gttgatctta
    28741 cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc gtcgttaacg
    28801 tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga tttctccaga
    28861 taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat cggcacaatt
    28921 tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac gtcgatcata
    28981 tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac gcgacgttta
    29041 gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac gttgttcagg
    29101 ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg ctgaccggtg
    29161 acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat ttccgacacc
    29221 cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc cagcgcacac
    29281 cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata caccgtttcg
    29341 acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga acgctgttct
    29401 tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg caacactttg
    29461 ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca cttcatcacc
    29521 tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat ctgctgacgg
    29581 ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg tgtgactaat
    29641 tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg gagggttgaa
    29701 aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta caaattctgg
    29761 cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg cgcgggcaca
    29821 cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg gaatcagaac
    29881 cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta ccggccagtg
    29941 cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg ttttcacctt
    30001 ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca tcaaagaacg
    30061 gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg tgcagcattt
    30121 tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta ccaatgacgt
    30181 tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc atttttacct
    30241 ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat tataatgagc
    30301 aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg agttttagca
    30361 acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat ttgccccacg
    30421 tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt cacgttgcgc
    30481 ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc ccctccactt
    30541 gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat cgcgccatcg
    30601 cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg gcaaattgtc
    30661 gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg ttccagcggt
    30721 agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc gacgcgctgc
    30781 cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg ctctgacagt
    30841 aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg gcgacagtta
    30901 ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg agtgactgag
    30961 ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt attgacctga
    31021 ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt gccgtcaggt
    31081 ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca ggcgtcacga
    31141 tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa ggcactatcc
    31201 ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc ccccgacgac
    31261 attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc tgccttgccg
    31321 tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc agcgccgccg
    31381 agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa tccaacattg
    31441 cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac agcaggtaag
    31501 ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc gctatcaaag
    31561 ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga tgccgggtgg
    31621 atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga tcctggcgat
    31681 cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag atcaaccgat
    31741 cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc cagtgagctg
    31801 cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg ttgaagaccg
    31861 gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat gcgcgaaaac
    31921 agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc ccttgactct
    31981 tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac tttattacgc
    32041 gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc ttgcggcaaa
    32101 accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc gagataagcc
    32161 caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt aagatacaag
    32221 gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca gagctggcga
    32281 attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg catagtgagc
    32341 gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc gcgcgccacc
    32401 gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt gatcagcgct
    32461 tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat gccgtcagca
    32521 tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc gggattgact
    32581 tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa aaggagaaat
    32641 ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg catttacggt
    32701 cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac atacattgat
    32761 tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc cagatacacc
    32821 agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc gtcacggaac
    32881 tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac ttcgccacca
    32941 ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc cagacccaca
    33001 ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac ctgcaaccac
    33061 accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc atcagtacca
    33121 agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga ttgcggataa
    33181 ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac ctgcaaggtg
    33241 acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag cggctcagct
    33301 gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg ggcagccagg
    33361 aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg ctgcgaaagt
    33421 gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag caaggagagc
    33481 attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg ctcatcacta
    33541 tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc atcacccatg
    33601 gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc tgcacgatgc
    33661 tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag caccagcgca
    33721 gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg gatcgacatc
    33781 atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat ggcgtctgtc
    33841 ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta agccgtcagc
    33901 cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc acgttgcatt
    33961 tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc actgtcgcct
    34021 ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa caggccgctg
    34081 gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata acgtgcaatg
    34141 ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg cgagaagttt
    34201 tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt accgtaatta
    34261 accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg ctgatcggca
    34321 acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc atcgccataa
    34381 cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc taattcaacg
    34441 gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc aacgttcagt
    34501 ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt atcgccactc
    34561 gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat cactttgctt
    34621 tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa ttcaccgtta
    34681 aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt taccgacagc
    34741 gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg tttcagctca
    34801 tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat aacctgaccg
    34861 taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg ccacggatct
    34921 ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac cagcacattc
    34981 acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt taatggttga
    35041 ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt ttcatcacca
    35101 agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga tttatcgcca
    35161 ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt atcgaccgga
    35221 atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag cggcccttca
    35281 ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg caacttaatg
    35341 gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc gggtcgcact
    35401 gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg cacgctactg
    35461 accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac cgggaaactg
    35521 accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat cagatctttt
    35581 tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta atcgcggcgt
    35641 tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg cgcatcgcta
    35701 taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt caccgtgctg
    35761 tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc cggcaatgca
    35821 tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc cgattccagc
    35881 aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg gctttctgta
    35941 gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag cgtgcgggaa
    36001 aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac agcctcacgc
    36061 agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc accgtacagg
    36121 tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt tttctcaccg
    36181 gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc ccacatccgg
    36241 tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc attgctatcg
    36301 agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt cctgagtacc
    36361 tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc tttaccgtct
    36421 gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag atcgcgtggg
    36481 ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa ttctgctaag
    36541 tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg tgccagcagt
    36601 aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact ggttgcctga
    36661 gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg ggccgcgccg
    36721 ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc tgaaacgccg
    36781 atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc agcctgattc
    36841 atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag cggcagcaat
    36901 aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta gaccagatcc
    36961 gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta ttcccattgg
    37021 ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc gacattatta
    37081 acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag cgaaccacgg
    37141 ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc gtaatcttta
    37201 ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat caaatcacgt
    37261 ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc ccaggcacca
    37321 tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc ctgatccggg
    37381 tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg gacttcagag
    37441 agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg tctctccggc
    37501 acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac ttcagaaggc
    37561 gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc cagcgccagc
    37621 attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca caatggcccg
    37681 ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc agtatcacgc
    37741 ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag cctctttcgc
    37801 ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa tggagatgcc
    37861 tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg atgacccgga
    37921 aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta acgttgctca
    37981 ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag cgctttctga
    38041 tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg tggcgatgcg
    38101 tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta atcttttctc
    38161 agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt cgattctggg
    38221 gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg cagtagagct
    38281 gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag taaccgatgt
    38341 attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg ctgcacgttt
    38401 taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc ccggagcact
    38461 gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg atgaactgga
    38521 tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca gctgcggctc
    38581 tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc caaacgtgaa
    38641 actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg ttgagccagt
    38701 gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt tattgctggt
    38761 ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc gtctcagcgg
    38821 agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt attgttctgt
    38881 tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg gcttaaggaa
    38941 aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc ggcaatatcg
    39001 ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat acggtgcttc
    39061 tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa ccggagtctc
    39121 tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc ccgaatgccg
    39181 ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc aaaaaaccac
    39241 cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta attccttttt
    39301 cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta tcggtcgcta
    39361 cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg ccaataagta
    39421 aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc ttcaccggct
    39481 taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc tctgcgactt
    39541 ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc ggatttccct
    39601 cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc ggcagtttgg
    39661 cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg ggcattacga
    39721 caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg aaaaaaggaa
    39781 tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta tcttcaacca
    39841 cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt agagtacgga
    39901 aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct tcaagttcgt
    39961 tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa atgcctgatg
    40021 cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag aatttgtagg
    40081 ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcta
    40141 tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc gctttacggt
    40201 aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca
    40261 ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt
    40321 cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga cgccagaatg
    40381 gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg tcaaaactga
    40441 acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat
    40501 caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca agcaccagac
    40561 gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag gtgatgatat
    40621 cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg aacagcttca
    40681 cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc
    40741 cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg
    40801 agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct ttatcgccag
    40861 ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt
    40921 gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac
    40981 gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca
    41041 attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat
    41101 ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc gtgcctttcg
    41161 gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct tcaccgctta
    41221 actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagcccca
    41281 gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg taagttgctt
    41341 tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca
    41401 ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta
    41461 aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat agataactat
    41521 gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga cgcggaaatt
    41581 attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg ttggatgcct
    41641 gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg gtgaatcgaa
    41701 ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat
    41761 cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt tgatagtgta
    41821 acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc ttaaacggct
    41881 ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg agctttctgg
    41941 cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac aggatttttc
    42001 acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg tttcaccgct
    42061 attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa taacaatctt
    42121 tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc gactggtcca
    42181 tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg
    42241 cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg tcaatgacct
    42301 gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc agactttcca
    42361 gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg tcctgctcgg
    42421 catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga ccgtaagacg
    42481 gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc acgctcaaaa
    42541 gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc ggtagcgccg
    42601 gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac
    42661 cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg aaatcctgag
    42721 cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg ccgcccatcg
    42781 tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg tctggcttgt
    42841 tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta tccgggtcga
    42901 tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc
    42961 gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca cccgcgtctt
    43021 taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc agttcattga
    43081 attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat
    43141 cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga acgcggttat
    43201 cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat
    43261 catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta
    43321 agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg atcacgcctt
    43381 cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta agtaagcgca
    43441 gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa
    43501 agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct tcagttgccc
    43561 gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc agccccgccg
    43621 ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc agatgcggat
    43681 agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa ctaatggtgt
    43741 tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt
    43801 gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac gtttcggcct
    43861 gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata ccagccgcca
    43921 gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat aaggccatgt
    43981 ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc actgctccgc
    44041 agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca cgcctcgttg
    44101 tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac acgtttgata
    44161 aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc
    44221 tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca
    44281 aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag cgtttgccag
    44341 gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg
    44401 gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct cagcgcctgg
    44461 gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat aacattctca
    44521 gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg cgcaggcgac
    44581 cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat
    44641 ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt acgaagtcca
    44701 gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc acgcctttgt
    44761 cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac
    44821 ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg cggttagcca
    44881 gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata accaaacctc
    44941 aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt gatcgcgtct
    45001 tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc agcaatatcg
    45061 gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc ggtgaccagg
    45121 gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc ttcaatgata
    45181 ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg tgcccccacc
    45241 atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg cggattctcg
    45301 taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc cgataccgat
    45361 taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat ttcccacagc
    45421 ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt gtagtcgatc
    45481 tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc cagctcgtcg
    45541 ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa
    45601 cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc aacgtagttg
    45661 aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag gtaaacttct
    45721 tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg
    45781 gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat ctggtgaaca
    45841 ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg
    45901 cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc atagattttg
    45961 tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg cagtttgccc
    46021 acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat ttcgccgata
    46081 gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga aacaaggatg
    46141 gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt acgctgcggt
    46201 gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc cagtaccgct
    46261 ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa gttggctgca
    46321 cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat ttcacgcgga
    46381 tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc ttcagcctgc
    46441 cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat cgtcataaac
    46501 tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta gtcgagataa
    46561 atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga accaggctac
    46621 cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat tctaatatct
    46681 tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac gaatctccgg
    46741 gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat gcaacacgaa
    46801 acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat gcgggtgcgt
    46861 ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt attaaccagt
    46921 tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc acgccacagc
    46981 gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca acgggtagca
    47041 tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct ggcatctttg
    47101 cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt tttacgcaga
    47161 cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga aatatcagcc
    47221 aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt cacggcatag
    47281 cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct gcccggggtt
    47341 ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc ccgagtaaat
    47401 tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc cattttttta
    47461 tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc aattattcgc
    47521 attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga ggggaccgtc
    47581 cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg ctctatagaa
    47641 gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg ggtaaacaga
    47701 cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc ggttgccagc
    47761 agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg atacggcgtc
    47821 tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat acgcacttca
    47881 taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg gtttgccgca
    47941 atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac gcgctcacga
    48001 ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag accgcattcg
    48061 cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc caccaccagg
    48121 ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc aatcacatcg
    48181 ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg attaaccagc
    48241 cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat attgccggtg
    48301 tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct tcatctaaag
    48361 attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc tatactctgc
    48421 gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat gctgaacatc
    48481 gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga aaccccggac
    48541 gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga taaagctgcc
    48601 gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat catcaccgaa
    48661 gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga tccactggat
    48721 ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc cgttcgtatc
    48781 aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact gttcaccgcc
    48841 actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac cgctcgcgat
    48901 ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta cgccactacc
    48961 tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg taccggttct
    49021 gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt tgaaatcggt
    49081 ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg cggcatcgtc
    49141 agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc tggtaacccg
    49201 cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc tctgaagcgt
    49261 taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc tattctgaaa
    49321 tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc cgattaatgg
    49381 atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg cgttctcaaa
    49441 attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg cgttgccatc
    49501 aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc ggcggaatcc
    49561 gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc ttcttcgacg
    49621 ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc cggaaatatg
    49681 tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt ttttatgttt
    49741 gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt gctggacgat
    49801 acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca acggctggtg
    49861 ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca ggatgatcgt
    49921 gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac catcgccaat
    49981 cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga aaattatatc
    50041 gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca cgttatccca
    50101 tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt gatcctaatc
    50161 ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta tcgcgaacag
    50221 atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat cgctagcacg
    50281 ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac agtttaagat
    50341 gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct tcgccatttc
    50401 cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac agatgatgcc
    50461 tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga tcttgctgac
    50521 gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta atggtgtgga
    50581 tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc gctatcatca
    50641 actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag tgcttaacca
    50701 acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt catttattac
    50761 cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg cgcgtaactt
    50821 ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc tgcaaaaacg
    50881 ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt tgctgcaaca
    50941 ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg aatcctgcct
    51001 tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc tccagcctgt
    51061 accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg ttcccgatcc
    51121 attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac gtctggtaaa
    51181 ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat gcgatcagct
    51241 ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta ttgataatac
    51301 cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc gcgcggcgct
    51361 ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg gtctggtcgc
    51421 ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac agattattct
    51481 actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac gcgaactaac
    51541 gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga caggcgctcc
    51601 gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct tttcaccacc
    51661 gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc gcaaaatgct
    51721 cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc gccaccagcg
    51781 ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg aaaacggtca
    51841 tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa cggatgactt
    51901 cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc gtgaaggtgc
    51961 cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct cccataatgc
    52021 cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca ctacaacggc
    52081 ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc agcgaccaca
    52141 aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg ctaaaaccgt
    52201 aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc cagttctggc
    52261 gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt gccccttgtg
    52321 gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc aacgtcaaca
    52381 gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc gccgagccaa
    52441 tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg aactgctttt
    52501 gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag aggttaaagc
    52561 caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg acagcaaaga
    52621 gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga tcgctgacgc
    52681 ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc aataacaggc
    52741 cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa ggtagaaaaa
    52801 tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg gattgcaaaa
    52861 ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt ggtgcaagtt
    52921 gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc acaagcacta
    52981 aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc gttacgcttg
    53041 ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc tcccctttct
    53101 tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga aaaacaacct
    53161 gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc cccatgccca
    53221 ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc tggctgtttt
    53281 cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc ggatcggtac
    53341 tgacgaaatt cacgccatcc agttacgcgg cggtgatctc tttttcatgt gctaaagggt
    53401 gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa tcaatctccg
    53461 ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga attttttcct
    53521 cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt ctgagacgaa
    53581 acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag ccaatggtta
    53641 attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc gcttgttcga
    53701 ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg actttgcgct
    53761 tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc tggctgctta
    53821 acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt gcctgcgcca
    53881 ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta tcgaaaaaac
    53941 gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca acatccttat
    54001 gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata tgaccacacc
    54061 ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca ctccgcgtat
    54121 ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc gttgctggtt
    54181 atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca cctacatggg
    54241 ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt ttctcaacca
    54301 atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc gcgcctttac
    54361 ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg taccgctgga
    54421 acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg aagttccttg
    54481 tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac cgggcctgcg
    54541 tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc gcgaaggcgg
    54601 caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga aattcccggc
    54661 agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg ccgttcaggt
    54721 attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa ccgcccgccc
    54781 ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg gtagcggttt
    54841 cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt caagctatta
    54901 ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc tgcgcctggc
    54961 gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca cgctccgcgt
    55021 ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta ctgacaaagc
    55081 cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt ttggtcctgc
    55141 tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat tgcttaaagg
    55201 ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg aaaagcgccg
    55261 cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg ctcgtggaat
    55321 gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc tcgataaaac
    55381 cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga gttacatcac
    55441 cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa atttcgtgac
    55501 tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt taacgcgcaa
    55561 acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa tgacaccaaa
    55621 gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc agaagagccg
    55681 ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga catcccaaac
    55741 gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg cgatgaaaca
    55801 ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa ctggcgcttg
    55861 ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct gagtgtactg
    55921 ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt gaggctctcc
    55981 ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt tatgccatta
    56041 acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa gatgacgcca
    56101 cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa gcgttatgcc
    56161 tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt gacattttca
    56221 tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt ttattaccgg
    56281 cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag gcgcgcaagt
    56341 tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat ttggcgaaca
    56401 tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg cggtcgatca
    56461 gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca tctgggatca
    56521 caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc acgagctgtt
    56581 taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg cgttaatcgc
    56641 cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg gcggcggtgg
    56701 cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac tggcttatga
    56761 actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca gcgacctgcg
    56821 cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga cgccggagaa
    56881 aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta cggggccgta
    56941 tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga tcaacgctga
    57001 tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc tttaaggaag
    57061 cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg caatggctgc
    57121 ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg atgagcgaca
    57181 tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt ccccgcagtt
    57241 acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc attccggtgt
    57301 aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg gcgaaagctg
    57361 gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc cgttgcttga
    57421 tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca gactgcgaga
    57481 agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg gtctggaact
    57541 ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg cggcaaccgt
    57601 catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc accagcaggc
    57661 tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg aaaaagtaga
    57721 actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg gtattggtat
    57781 cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg gcattgtcat
    57841 tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg tggcaatcac
    57901 tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca ataaccaggc
    57961 gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc cgccgtggtt
    58021 ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg gcgatgactg
    58081 gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc aaaacggcgt
//
LOCUS       DAJTVE010000008        57419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000008 DAJTVE010000000
VERSION     DAJTVE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            134..1477
                     /gene="argG"
                     /locus_tag="OO439_000479"
     CDS             134..1477
                     /gene="argG"
                     /locus_tag="OO439_000479"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6314942.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1485..3092)
                     /locus_tag="OO439_000480"
     CDS             complement(1485..3092)
                     /locus_tag="OO439_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6314943.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3570..3656)
                     /locus_tag="OO439_000481"
     tRNA            complement(3570..3656)
                     /locus_tag="OO439_000481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3620..3622),aa:Leu,seq:gag)
     gene            complement(3671..4003)
                     /gene="secG"
                     /locus_tag="OO439_000482"
     CDS             complement(3671..4003)
                     /gene="secG"
                     /locus_tag="OO439_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6314944.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4231..5568)
                     /gene="glmM"
                     /locus_tag="OO439_000483"
     CDS             complement(4231..5568)
                     /gene="glmM"
                     /locus_tag="OO439_000483"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6314945.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5561..6409)
                     /gene="folP"
                     /locus_tag="OO439_000484"
     CDS             complement(5561..6409)
                     /gene="folP"
                     /locus_tag="OO439_000484"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6314946.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6499..8433)
                     /gene="ftsH"
                     /locus_tag="OO439_000485"
     CDS             complement(6499..8433)
                     /gene="ftsH"
                     /locus_tag="OO439_000485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6314947.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8533..9162)
                     /gene="rlmE"
                     /locus_tag="OO439_000486"
     CDS             complement(8533..9162)
                     /gene="rlmE"
                     /locus_tag="OO439_000486"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6314948.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9288..9581
                     /gene="yhbY"
                     /locus_tag="OO439_000487"
     CDS             9288..9581
                     /gene="yhbY"
                     /locus_tag="OO439_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6314949.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9737..10213)
                     /gene="greA"
                     /locus_tag="OO439_000488"
     CDS             complement(9737..10213)
                     /gene="greA"
                     /locus_tag="OO439_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6314950.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10461..11894
                     /gene="dacB"
                     /locus_tag="OO439_000489"
     CDS             10461..11894
                     /gene="dacB"
                     /locus_tag="OO439_000489"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6314951.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12080..13252)
                     /gene="cgtA"
                     /locus_tag="OO439_000490"
     CDS             complement(12080..13252)
                     /gene="cgtA"
                     /locus_tag="OO439_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6314952.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13268..14233)
                     /locus_tag="OO439_000491"
     CDS             complement(13268..14233)
                     /locus_tag="OO439_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6314953.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14360..14617)
                     /gene="rpmA"
                     /locus_tag="OO439_000492"
     CDS             complement(14360..14617)
                     /gene="rpmA"
                     /locus_tag="OO439_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6314954.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14638..14949)
                     /gene="rplU"
                     /locus_tag="OO439_000493"
     CDS             complement(14638..14949)
                     /gene="rplU"
                     /locus_tag="OO439_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6314955.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15208..16179
                     /gene="ispB"
                     /locus_tag="OO439_000494"
     CDS             15208..16179
                     /gene="ispB"
                     /locus_tag="OO439_000494"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6314956.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16408..16686
                     /gene="sfsB"
                     /locus_tag="OO439_000495"
     CDS             16408..16686
                     /gene="sfsB"
                     /locus_tag="OO439_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6314957.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16734..17993)
                     /gene="murA"
                     /locus_tag="OO439_000496"
     CDS             complement(16734..17993)
                     /gene="murA"
                     /locus_tag="OO439_000496"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6314958.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18048..18302)
                     /gene="ibaG"
                     /locus_tag="OO439_000497"
     CDS             complement(18048..18302)
                     /gene="ibaG"
                     /locus_tag="OO439_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6314959.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18462..18755)
                     /gene="mlaB"
                     /locus_tag="OO439_000498"
     CDS             complement(18462..18755)
                     /gene="mlaB"
                     /locus_tag="OO439_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6314960.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18755..19390)
                     /gene="mlaC"
                     /locus_tag="OO439_000499"
     CDS             complement(18755..19390)
                     /gene="mlaC"
                     /locus_tag="OO439_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6314961.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19409..19960)
                     /gene="mlaD"
                     /locus_tag="OO439_000500"
     CDS             complement(19409..19960)
                     /gene="mlaD"
                     /locus_tag="OO439_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6314962.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19965..20747)
                     /gene="mlaE"
                     /locus_tag="OO439_000501"
     CDS             complement(19965..20747)
                     /gene="mlaE"
                     /locus_tag="OO439_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6314963.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20755..21564)
                     /gene="mlaF"
                     /locus_tag="OO439_000502"
     CDS             complement(20755..21564)
                     /gene="mlaF"
                     /locus_tag="OO439_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6314964.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21774..22751
                     /locus_tag="OO439_000503"
     CDS             21774..22751
                     /locus_tag="OO439_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6314965.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22765..23751
                     /gene="kdsD"
                     /locus_tag="OO439_000504"
     CDS             22765..23751
                     /gene="kdsD"
                     /locus_tag="OO439_000504"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6314966.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23772..24338
                     /gene="kdsC"
                     /locus_tag="OO439_000505"
     CDS             23772..24338
                     /gene="kdsC"
                     /locus_tag="OO439_000505"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6314967.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24335..24910
                     /gene="lptC"
                     /locus_tag="OO439_000506"
     CDS             24335..24910
                     /gene="lptC"
                     /locus_tag="OO439_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6314968.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24879..25436
                     /gene="lptA"
                     /locus_tag="OO439_000507"
     CDS             24879..25436
                     /gene="lptA"
                     /locus_tag="OO439_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6314969.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25443..26168
                     /gene="lptB"
                     /locus_tag="OO439_000508"
     CDS             25443..26168
                     /gene="lptB"
                     /locus_tag="OO439_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6314970.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26216..27649
                     /gene="rpoN"
                     /locus_tag="OO439_000509"
     CDS             26216..27649
                     /gene="rpoN"
                     /locus_tag="OO439_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6314971.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27672..27959
                     /gene="hpf"
                     /locus_tag="OO439_000510"
     CDS             27672..27959
                     /gene="hpf"
                     /locus_tag="OO439_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6314972.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28077..28568
                     /gene="ptsN"
                     /locus_tag="OO439_000511"
     CDS             28077..28568
                     /gene="ptsN"
                     /locus_tag="OO439_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6314973.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28614..29468
                     /gene="rapZ"
                     /locus_tag="OO439_000512"
     CDS             28614..29468
                     /gene="rapZ"
                     /locus_tag="OO439_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6314974.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29465..29737
                     /gene="npr"
                     /locus_tag="OO439_000513"
     CDS             29465..29737
                     /gene="npr"
                     /locus_tag="OO439_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6314975.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29951..30583
                     /gene="yrbL"
                     /locus_tag="OO439_000514"
     CDS             29951..30583
                     /gene="yrbL"
                     /locus_tag="OO439_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6314976.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30580..31308)
                     /gene="mtgA"
                     /locus_tag="OO439_000515"
     CDS             complement(30580..31308)
                     /gene="mtgA"
                     /locus_tag="OO439_000515"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6314977.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31305..31958)
                     /gene="elbB"
                     /locus_tag="OO439_000516"
     CDS             complement(31305..31958)
                     /gene="elbB"
                     /locus_tag="OO439_000516"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6314978.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32188..34524)
                     /gene="arcB"
                     /locus_tag="OO439_000517"
     CDS             complement(32188..34524)
                     /gene="arcB"
                     /locus_tag="OO439_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6314979.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34620..35549)
                     /locus_tag="OO439_000518"
     CDS             complement(34620..35549)
                     /locus_tag="OO439_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6314980.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36224..40684
                     /gene="gltB"
                     /locus_tag="OO439_000519"
     CDS             36224..40684
                     /gene="gltB"
                     /locus_tag="OO439_000519"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6314981.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40697..42115
                     /gene="gltD"
                     /locus_tag="OO439_000520"
     CDS             40697..42115
                     /gene="gltD"
                     /locus_tag="OO439_000520"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6314982.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42299..42778
                     /locus_tag="OO439_000521"
     CDS             42299..42778
                     /locus_tag="OO439_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6314983.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42795..43100
                     /locus_tag="OO439_000522"
     CDS             42795..43100
                     /locus_tag="OO439_000522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6314984.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43176..43640)
                     /locus_tag="OO439_000523"
     CDS             complement(43176..43640)
                     /locus_tag="OO439_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6314985.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43637..44512)
                     /gene="nanK"
                     /locus_tag="OO439_000524"
     CDS             complement(43637..44512)
                     /gene="nanK"
                     /locus_tag="OO439_000524"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6314986.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44509..45198)
                     /locus_tag="OO439_000525"
     CDS             complement(44509..45198)
                     /locus_tag="OO439_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6314987.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45246..46736)
                     /gene="nanT"
                     /locus_tag="OO439_000526"
     CDS             complement(45246..46736)
                     /gene="nanT"
                     /locus_tag="OO439_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6314988.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46846..47739)
                     /gene="nanA"
                     /locus_tag="OO439_000527"
     CDS             complement(46846..47739)
                     /gene="nanA"
                     /locus_tag="OO439_000527"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6314989.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47861..48652)
                     /gene="nanR"
                     /locus_tag="OO439_000528"
     CDS             complement(47861..48652)
                     /gene="nanR"
                     /locus_tag="OO439_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6314990.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49032..50354
                     /gene="dcuC"
                     /locus_tag="OO439_000529"
     CDS             49032..50354
                     /gene="dcuC"
                     /locus_tag="OO439_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6314991.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50397..50894)
                     /gene="sspB"
                     /locus_tag="OO439_000530"
     CDS             complement(50397..50894)
                     /gene="sspB"
                     /locus_tag="OO439_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6314992.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50900..51538)
                     /gene="sspA"
                     /locus_tag="OO439_000531"
     CDS             complement(50900..51538)
                     /gene="sspA"
                     /locus_tag="OO439_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6314993.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51933..52325)
                     /gene="rpsI"
                     /locus_tag="OO439_000532"
     CDS             complement(51933..52325)
                     /gene="rpsI"
                     /locus_tag="OO439_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6314994.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52341..52769)
                     /gene="rplM"
                     /locus_tag="OO439_000533"
     CDS             complement(52341..52769)
                     /gene="rplM"
                     /locus_tag="OO439_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6314995.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52988..54115)
                     /gene="zapE"
                     /locus_tag="OO439_000534"
     CDS             complement(52988..54115)
                     /gene="zapE"
                     /locus_tag="OO439_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6314996.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54309..54707
                     /locus_tag="OO439_000535"
     CDS             54309..54707
                     /locus_tag="OO439_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6314997.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54861..56228
                     /gene="degQ"
                     /locus_tag="OO439_000536"
     CDS             54861..56228
                     /gene="degQ"
                     /locus_tag="OO439_000536"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6314998.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56318..57385
                     /gene="degS"
                     /locus_tag="OO439_000537"
     CDS             56318..57385
                     /gene="degS"
                     /locus_tag="OO439_000537"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6314999.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14261 a        15301 c        14253 g        13604 t
ORIGIN      
        1 tacaaaaaac acactaaact cttcctgtct ccgataaaag atgattaaat gaaaactcat
       61 ttattttgca taaaaattca gtgagagcgg aaatccaggc tcatcatcag ttaattaagc
      121 agggtgttat tttatgacga cgattctcaa gcatctcccg gtaggtcaac gtattggtat
      181 cgctttttcc ggcggtctgg acaccagtgc cgcactgctg tggatgcgac aaaagggagc
      241 ggttccttat gcatatactg caaacctggg ccagccagac gaagaggatt atgatgcgat
      301 ccctcgtcgt gccatggaat acggcgcgga gaacgcacgt ctgatcgact gccgcaaaca
      361 actggtggcc gaaggtattg ccgctattca gtgtggcgca tttcataaca ccaccggcgg
      421 cctgacctat ttcaacacga cgccgctggg ccgcgccgtg actggtacca tgctggttgc
      481 tgcgatgaaa gaagatggcg tgaatatctg gggtgacggt agcacctata aaggaaacga
      541 tatcgaacgt ttctatcgtt atggtctgct gaccaatgct gaactgcaga tttacaaacc
      601 gtggcttgat actgacttta ttgatgaact gggcggccgt catgagatgt ctgaatttat
      661 gattgcctgc ggtttcgact acaaaatgtc tgtcgaaaaa gcctactcca cagactccaa
      721 catgcttggt gcaacgcacg aagcgaagga tctggaatac ctcaactcca gcgtcaaaat
      781 cgtcaaccca attatgggcg tgaaattctg ggatgagagc gtgaagatcc cggcagaaga
      841 agtcacagta cgctttgagc aaggtcatcc ggtagcgctg aacggtaaaa cctttagcga
      901 cgacgtagaa atgatgctgg aagctaaccg catcggcggt cgtcacggcc tgggcatgag
      961 cgaccagatt gaaaaccgta tcatcgaagc gaaaagccgt ggtatttacg aagctccggg
     1021 gatggcactg ctacatatcg cttatgaacg tctgttgacc ggtattcaca acgaagacac
     1081 cattgagcag tatcatgcac atggtcgtca gttgggccgt ctgctgtacc aggggcgttg
     1141 gtttgattcc caggcgctga tgctgcgtga ctctctgcaa cgctgggttg ccagccagat
     1201 cactggtgaa gttaccctgg agctgcgccg tgggaacgat tattcaatcc tgaataccgt
     1261 ctcagagaac ctgacctaca agccagagcg tctgacgatg gaaaaaggcg actcggtgtt
     1321 ctcgccagat gatcgtattg gtcaattgac catgcgtaac ctggatatca ctgatacccg
     1381 agagaaactt tttggttatg ccaaaactgg cctgctctcc tcctctgcca cttcaggcgt
     1441 gccgcaggtg gagaatctgg aaaacaaagg ccagtaattc gacatcaacc ctgcccttca
     1501 ggggtggggt taccttgctt atcctctgta aactcctttc tgagcatcac ataatcgaaa
     1561 acatcatggt ttgcatccac aacccgtgag tctcctttat agtgtgcaat cacttcctcc
     1621 agcgttttcg gttgttccgg ctttgttacc cccatccacg cattaataag gtaattattg
     1681 taagctgtcg aaaagatgtt gttttccgtt gtacgatcca cgccatcccc aagaacgggg
     1741 ctataccaga taaacatcgg gacatgatat gcctgctggc tagcctccct gcctccatga
     1801 aaatagacgt tcttcttcgt agggtcacgt tccaggccgt ggtcggcaaa atacatgacc
     1861 gaggcgcggc gatcttttaa taattcaaaa acctgaccta gcaaactatc tgtgtaatga
     1921 atggagttgt catagcaggc atcctgatcg tcctgcggtt gaaacacggc gctggattgc
     1981 ggataggcgc tacaagccgg ttcatggctt ccatttaaat gaagaacaat cagtttcttt
     2041 tgctgcgtat tttgctgtaa tgcttgcgat aaatgcggca acaacaattc atcaaatcct
     2101 ctgacataaa ctgtttccat ggcgcgcatg gcgatactgg taactgctgt accattctgc
     2161 cgaaaagcgg attgcgagct tagccagaaa gtctgaaatc ctgcttgatt agccatatta
     2221 ataatgttgt cggggtaatt atgaatgtca tgactcaaaa cagaatcagc agttaaagaa
     2281 aggggaaccg acagcgcagt gtaaggtgcg ccgcttattg attgattaaa cagtttgatc
     2341 tgttttcttt gtgcttcaac ttgcggtgtc gtagagcgtg tatatccata caaagacata
     2401 ttgtcgacac gtacagactc ccccacaatc aacacgtagg tatcaatacc tgtatccctg
     2461 actgataatt gaaaatacgg caccgtgttt gcaattgaga gtaatctttg atgctctttc
     2521 gctgctaaag caaaataatt gagattgaaa aacggcgtat aggtagcaaa tcgcgacgct
     2581 agtatatatg gactgaatgc ttttttattt tttgcatctt tataagcaaa ttgacaagca
     2641 gaaaataaac tgcccgaaat gacaatcagc aataatattc ctgtcacttt ttttgtcggc
     2701 aaggaaacat catattttat tattactgcc aaaaaaagaa gggataaaaa agcaaaagca
     2761 catagataag ggatatacat ccccagcatt ttgacaactt catccggatc actctgcagc
     2821 acactaatcg caaatccatc attgaaagtt gtaccaaagg tacaccatga atagaggcta
     2881 atactcatgt ctgccgcagt cagtaacaca aatggtgcgg caacaagcag gcgaagcgga
     2941 agggatgata tacgttttac aaccaggatg gtcaggacga agaacagcaa tcgctgaacc
     3001 atatgcgcgc cggaagaggc gactaagttc gtaataccaa aaacaataac acaaagatac
     3061 aacaaccaat gagatttaaa atcttctagc aaatttgttg aatactgtca tccagaaatc
     3121 caagttcaca accaatataa acaattatca acaatgttca attttatatc agacatgttt
     3181 ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta aatgatgcgg
     3241 ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat tacgcagaac
     3301 gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt aaacatatcc
     3361 gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag cagaagggaa
     3421 aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac gagtaaagca
     3481 actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa acgaaaaaag
     3541 acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg aacccgtaag
     3601 ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac ctcggcacgg
     3661 atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct ggctgagttt
     3721 gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg gttttgttgc
     3781 tattgatgtt acccagcacc agactgatga taaagaataa cgttgccagc agcgccgtca
     3841 tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa gcgcctgctc
     3901 cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga ccaacaaggc
     3961 caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac ctgttccttg
     4021 cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat ctttcccact
     4081 gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt ttatcgcatt
     4141 gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa aggcgacaat
     4201 cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat cggcgatacg
     4261 gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc gaattaacgg
     4321 ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg cttcaacttc
     4381 ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg cggtgtaacg
     4441 cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt gcaggctcat
     4501 atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt caccggtagt
     4561 ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc agcctttctc
     4621 ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg gaattcctaa
     4681 ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag caccaccacg
     4741 cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat caccatcgac
     4801 tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga aggcaatacc
     4861 gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg tagcccccac
     4921 ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt tcgcccccag
     4981 ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat ccaccacaat
     5041 cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact cgatatagcg
     5101 acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa cacagctgat
     5161 ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt tggtgccgtc
     5221 gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag atatcacaat
     5281 tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg gcatcgggcc
     5341 agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg actccagcat
     5401 atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt ggcgcgccag
     5461 cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg gcgcatcccc
     5521 tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag cgtttgtttt
     5581 cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct ttgacgtcat
     5641 gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc agactaccgc
     5701 tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt cgtgacatac
     5761 ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt aatgaatagt
     5821 tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc tcttttgcga
     5881 tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact tctgcaaaga
     5941 catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata tgcatcagac
     6001 aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa agggagcgga
     6061 tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct ggtttggatg
     6121 tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca ggaataacac
     6181 gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac tcgccaccaa
     6241 cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc gcatctatca
     6301 gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg atccccatta
     6361 cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg gtgttatccc
     6421 tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg tacaaataca
     6481 gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt tacccgggtt
     6541 cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt cgccagaatt
     6601 gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac gtgccatcag
     6661 gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt tcatcgcatg
     6721 cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac gctcaatcag
     6781 tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt tcgctttcgc
     6841 tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg gacccaattt
     6901 ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa ctttaatatc
     6961 gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg ccagacgacc
     7021 accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga ttgcgtcgcc
     7081 ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg tcactttgtg
     7141 caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt cgtggtaagc
     7201 cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac ccatcatgat
     7261 tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc cacgagcagc
     7321 gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac caggagtacc
     7381 acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca tgtgaacttt
     7441 caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac ggtcgaaacg
     7501 gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg cgataacgat
     7561 gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca gagtctgttc
     7621 acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg cgtcgatttc
     7681 atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt cacgaacacg
     7741 ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag taaagaacgg
     7801 aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac ccggaggacc
     7861 gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc tcggctcgcg
     7921 cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg caacgtcagc
     7981 aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac caaacgacat
     8041 ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca caccaatcag
     8101 caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt cttcaggcgg
     8161 ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg gatcctgaac
     8221 cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt cacgtccgtt
     8281 gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag agtaatccac
     8341 cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca gcacaacggc
     8401 aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa cctcttatta
     8461 caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa actttcgtct
     8521 gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc gcgaacgtgc
     8581 acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa tttcccttag
     8641 atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac ctggcgctaa
     8701 tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg ggatatccac
     8761 cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga ctttgctgtc
     8821 gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc cctgaagaaa
     8881 gtctacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc ggcctttgcc
     8941 gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa ggtcgacaac
     9001 cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt taaaccaggc
     9061 acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa agtgttcctg
     9121 aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt cccatgggga
     9181 taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata tatgggagat
     9241 ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg aatctgagta
     9301 ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt gttctgcttg
     9361 gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta gagcaccatg
     9421 aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg atcgtggaag
     9481 ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg ctggtgcttt
     9541 atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct aaagttacac
     9601 acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg ccacgctctg
     9661 ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca tcaacatctt
     9721 gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct acttcgccgc
     9781 ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca cgagcaatag
     9841 gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca acgatgcgat
     9901 aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca aaaataacgc
     9961 gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc ttggcttcga
    10021 tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg tattcggcgt
    10081 tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt tcaggacggc
    10141 gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt aaggtcatcg
    10201 gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc ttcacccacc
    10261 ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa aaaataccga
    10321 cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt taccctgaag
    10381 ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac ggctcgattc
    10441 caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg accagctgta
    10501 tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc cccgctggtg
    10561 ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt gattaccaca
    10621 gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg gcggcgttga
    10681 ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc aatgtggaaa
    10741 acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg ttaaaacgtc
    10801 aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa atcgatggca
    10861 atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc tggccatgga
    10921 atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac cgcaactgtt
    10981 tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata cgcgtggcat
    11041 cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt tctgccgaag
    11101 cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg ctgacgggat
    11161 gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat ggagccagtt
    11221 atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg agcggaacac
    11281 tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa cagtcggccc
    11341 cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc gccgatacgg
    11401 ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg gccgggtcgg
    11461 acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac accattattg
    11521 ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg atgcaggtgc
    11581 tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg ccgctggcgg
    11641 gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg gatggaaaag
    11701 tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc attaccacag
    11761 cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta gaacctgcgg
    11821 atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat aaagatattt
    11881 atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag attattgaca
    11941 aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta caaaatcgtg
    12001 caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat caggctgatt
    12061 tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct tcgtcgtctt
    12121 cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca agctgctggc
    12181 gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct tcagcctgca
    12241 cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc acgcccagtc
    12301 cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca gcaatcgctt
    12361 tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac actaaccaac
    12421 gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata atacgcgcgt
    12481 tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac aggacgcggc
    12541 aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca gcgccttcaa
    12601 tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt accacaccca
    12661 gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc gccgataccg
    12721 cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg tcagccagca
    12781 gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt ttctgccgtg
    12841 gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag ccgcccttag
    12901 caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca gtaccctggt
    12961 cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac
    13021 aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca aaacgataat
    13081 cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca tcaccaccgt
    13141 cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc acgcaaccat
    13201 taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc attttattct
    13261 ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta cgtaatccgc
    13321 cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca aacgcaccga
    13381 gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc agtgataaaa
    13441 gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg
    13501 cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta ttcagtccgc
    13561 aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca ggctttgcca
    13621 gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc ggtgaggcca
    13681 gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc gccgcaccga
    13741 ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg accagactgg
    13801 tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc
    13861 gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc gagagttgcc
    13921 caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac aggatgaagt
    13981 tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca
    14041 cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca atactcgcca
    14101 tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc tgcttcattg
    14161 cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg ccaatgcctg
    14221 cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc
    14281 gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg
    14341 ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg tttttcgggc
    14401 ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc
    14461 aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg atgctacccg
    14521 ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac
    14581 cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc tcaggtctta
    14641 ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatgaccct gctgcttacg
    14701 atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac cgtgagcaac
    14761 aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga cttcttcacc
    14821 gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga tgtccagctt
    14881 ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac tttggaaaac
    14941 cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga gtgcgctata
    15001 aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa gtgctacagt
    15061 caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc cattttttca
    15121 gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca cagtaaggta
    15181 atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg
    15241 caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga cgtccaactg
    15301 atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg tccgatgatt
    15361 gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac cattgctgcc
    15421 ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga tgaatcagat
    15481 atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag cgtgctggta
    15541 ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc actcaaagtg
    15601 ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct gcaactgatg
    15661 aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta tagcaaaacc
    15721 gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg tacgccggag
    15781 gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca gttgatcgac
    15841 gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg cgacgatctg
    15901 aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac accagaacag
    15961 gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct ggaaccggtt
    16021 ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc cgaggaagaa
    16081 gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg agaagcactc
    16141 atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt catcccgcgc
    16201 agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct ataacacctg
    16261 aaaaaaaagg gcgatgaatc tttatatatt ctgaatattc acactcttta caggaacttt
    16321 ttagagcaat aggccatcag gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa
    16381 agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg gcatcccgct
    16441 gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggagtc tcgcagaaat
    16501 ggtttgagtt cctcaacgct ggcaaacgca ttatcgcgcc catggccgaa aggagagatg
    16561 attattgcga aagccctggg aactgacccc tgggttatct ggccatcacg ctaccatgat
    16621 ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac taaaccgaaa
    16681 aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga cgattactcg
    16741 cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag
    16801 ccacgatcga tgtgataaat acgatcaacc accgtcgtcc cttccgcaat acagccagcc
    16861 agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc agaaagtttt
    16921 tcaacaccgt gacaaataac ggtattgctt tcgatttcgg catgcgcgcc catacggcta
    16981 agctccggca catgcataaa gcggttttca aagaccgttt cggtgatgaa tccggtccct
    17041 tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc
    17101 ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag gctaatccag
    17161 tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac ggcgtcgaga
    17221 gtatctggct gcgcgttacg gcagataatt ttgccgcgag aaatcgccgc cgccaccagg
    17281 aaagtaccgg tttcgatacg atccggtagc acgcgataga caccgccgcc taaacgttcc
    17341 acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc
    17401 aggaagttcg cggtatcgac gatttccggt tcacgcgctg cattttcaat aatcgtggta
    17461 ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct gactttatcc
    17521 atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta accttcttcc
    17581 agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca
    17641 ccgatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc taccagcggc
    17701 cccagtgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc gcagaataca
    17761 ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc acccagctgg
    17821 cttagcagct tcattgatgt atcgacgtct ttcagttttg ggacgttctg gatctctacc
    17881 ggttcttccg ccagtagagc ggcaaaaagg ataggcagag cagcattttt agcgccggaa
    17941 attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc catttagttt
    18001 gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca aaagccgttc
    18061 agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac agcatgaatg
    18121 cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt tttaacccga
    18181 ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc atcgccggaa
    18241 acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc attattttcc
    18301 atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa agttgcgctg
    18361 gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg caggcaggtt
    18421 tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg cagaacatca
    18481 gcaggcaaat tatacaattt cgcgagggta tacactttgt cgtttacccc ctgaagcgtc
    18541 acgttgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc cagtccaccc
    18601 gtatccacgc gggacacacg gctaagatcg atgcaggtaa tccccttcac cgcttcctca
    18661 cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa cgccagcgtg
    18721 tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc agagtgattt
    18781 tctgttgaga aatcgatttc agttgcgcag tcaggccgtc gatacctttg gtacgcagca
    18841 gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca atcatgtcgt
    18901 aagcctgcca gtttcccgtc tgggagtttt tacgccactg gaagtccaga cgcaccggcg
    18961 gacggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta tcgcccagcg
    19021 gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc tgaccgtaag
    19081 cctgcttcag gtactcacgg aaagcggcaa agtaggcttc acgttgagca ggggtcgcac
    19141 tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg tatggcagca
    19201 gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgc ggttgctcat
    19261 tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttataa ggattggtct
    19321 ggtctgccgc ggttgccgca ctcagaggtg caatcaccag caaagcgacc atcattaaac
    19381 gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt tcagtggttt
    19441 cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca tcgcctttac
    19501 taccgtaaag gaactgacca atgagatctt ccagcaccat cgcagactta gtgtcctgaa
    19561 ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta
    19621 atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa ctggtatctg
    19681 gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag gttttcgggt
    19741 ccagcgtaat atccgccacc cgacccacaa caacgccacc aatactgacc ggagagcgcg
    19801 ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta
    19861 tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagcagt gctgctaata
    19921 aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt cccaaacatc
    19981 aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac aacggtgcga
    20041 gtggttgccc ggctaatccc ggcagacgtc gggatggtgt cgtagccgtt aaacaacgaa
    20101 atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac cagatccata
    20161 cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat gcctttccaa
    20221 ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac cgtcaacagt
    20281 ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag cggatccacc
    20341 gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat ttctgcggtt
    20401 agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc
    20461 agtagcgata acgccaccag catacccaga ctggtttccg cactataagt ggtcagaacc
    20521 agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac aataatcagc
    20581 atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg tttgcgaaac
    20641 tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa cgttctcagg
    20701 gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag tggcttaact
    20761 ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa caggcccgtc
    20821 agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca acgcctgggc
    20881 actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatccg caatacttaa
    20941 cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc tgttcagctc
    21001 agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa aaggttcatc
    21061 aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat
    21121 cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca cggcctccag
    21181 cttcatcatc accgtactat gcaacaatgg cgcgggtagt tgggtatgtt cgcgcagtgg
    21241 ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gccccggact gaaataacat
    21301 gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa tattctcacc
    21361 atcgaaaagg atctcaccat gatctggtgc gatttgcccg ccaatcagac ggagtagcgt
    21421 cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg gcacggtcag
    21481 ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac
    21541 taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt ttctaagcat
    21601 ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc gtggttagcg
    21661 aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa tccgttacgg
    21721 ctttctgaaa atcttcagcg gaccgacgag tatacctgaa gaaaggacgt tagatgcttt
    21781 tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg
    21841 tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctgatc atcggcatga
    21901 cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc
    21961 acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc aatattttgc
    22021 tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattccgat gttctgcgtc
    22081 gtgaattacc cttaatgttg ttggtcagcg tagtggccgg ttccgtactc tatgacggac
    22141 aacttagtcg cagcgatggt atctttctcc tctttctggc tgtgctatgg ctgctgttca
    22201 ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc agagagcagc
    22261 ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc
    22321 ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt
    22381 actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagcctgc
    22441 cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa
    22501 atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc gcgctgataa
    22561 cgccaggaga gattgagcca ttggcgtaca gtcgtgacta cagcgtgatg ttgctggtga
    22621 gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg
    22681 tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac
    22741 tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt
    22801 cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa
    22861 tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt
    22921 gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc
    22981 agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc
    23041 atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc
    23101 acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc
    23161 ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa
    23221 gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat
    23281 gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca
    23341 cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg
    23401 ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcgtt gctggaagtt
    23461 acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat tgaaggcatc
    23521 tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt
    23581 attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag
    23641 gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat
    23701 ttactcggag tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag
    23761 gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg
    23821 ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc gatggcgtac
    23881 tgtcagatgg cctgatttat atgggcaata atggcgaaga gctgaaagcg ttcaatgttc
    23941 gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg
    24001 ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc
    24061 aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg gcgattgccc
    24121 cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg
    24181 gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc gattacgtga
    24241 cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta ctcctggcgc
    24301 agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat
    24361 cattgtgcta tcactggcgg ttctggtgat gatcggcatt gatatggccg aaaaagacga
    24421 taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac
    24481 gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata
    24541 ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga
    24601 taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct
    24661 ctatttatat ggacacgttg aagtcaacgc acttgtgcca aactctcaac ttcgcagaat
    24721 cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt
    24781 cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag
    24841 caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac
    24901 tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg
    24961 taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc
    25021 aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc atcaaaatta
    25081 atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg
    25141 gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc
    25201 acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt
    25261 atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg gtaaaagagc
    25321 agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc
    25381 agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt aattaattcg
    25441 ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag
    25501 aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg
    25561 ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat gcgggcaaca
    25621 tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta
    25681 tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga
    25741 tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg
    25801 agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg
    25861 ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg aaatttattc
    25921 tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca
    25981 ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa
    26041 cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca
    26101 cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca
    26161 gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa
    26221 gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc
    26281 aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga
    26341 gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca
    26401 agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct
    26461 gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg
    26521 tgactacatt gacgatgagc tgccggtcta tcagggcgaa acgacgcaga ccttgcagga
    26581 ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac
    26641 ctctatcgtc gatgccgttg atgacatcgg ttatctgact gtcccgctgg aagatattct
    26701 cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc ttaagcggat
    26761 ccaacggttt gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca
    26821 actctcccaa ttcgataaaa ccacgccgtg gctggaagag gccagactga tcattagcga
    26881 tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa
    26941 agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca
    27001 gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa
    27061 cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca
    27121 ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa
    27181 tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata cgctactgcg
    27241 cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg aagaatatat
    27301 gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc
    27361 tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt
    27421 cttttccagt cacatcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc
    27481 gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt
    27541 aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg
    27601 agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata
    27661 aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc
    27721 gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg
    27781 tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa
    27841 acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct
    27901 taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat
    27961 tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac
    28021 ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga
    28081 caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc
    28141 gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac
    28201 aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca
    28261 gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa gatactctgc
    28321 gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc
    28381 aaccggtgga cttacttttt gccctgctgg tgtcggcaga ccaaactaaa acgcacctgc
    28441 atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg
    28501 cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg
    28561 aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac
    28621 tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag
    28681 atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa
    28741 ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt
    28801 caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactcc
    28861 tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc
    28921 atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc
    28981 tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg
    29041 agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa ctgaccatgg
    29101 tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg
    29161 tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata
    29221 aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc
    29281 gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacttgacgg
    29341 tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag
    29401 actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta
    29461 aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc
    29521 tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt tacgtaatga
    29581 cgaaggcacc gaggccgaag ccaacagcgt tattgcgctg ctgatgttgg attctgccaa
    29641 aggacggcag attgaagttg aagcgaccgg cccacaggaa gaggaagcac tggccgccgt
    29701 tatcgccctc tttaactctg gttttgatga agattaatct tcatcacttt gacatacaac
    29761 tcccttcaaa ctccccccga caataagaaa atcacgtact gaaatcgttc tcaatcaacg
    29821 tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg
    29881 cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc
    29941 aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa
    30001 atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc atcgtggcga
    30061 tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt
    30121 aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg
    30181 ttatgtctac gatgtgattg cagattttga cggcaaactc tccattacgc tgaccgaatt
    30241 tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac tgaaacagct
    30301 gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct
    30361 ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag
    30421 cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt
    30481 atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc
    30541 tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgttgttg
    30601 cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg
    30661 cacgtagccc gatggcgagg agactttgaa acgaagtgga ttaggtaata cagctgccag
    30721 taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc
    30781 agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat
    30841 acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg
    30901 tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag aaatcgttga
    30961 agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct caatggaagc
    31021 gacatcaaag ccccagtgct cagaaaattt ctgatcttct gcggcaatca ccgccagtcc
    31081 catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt
    31141 gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg gaacaggcgc
    31201 aacactaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca ttaacagcaa
    31261 aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc agccagaacc
    31321 agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc
    31381 atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc atcgacagga
    31441 cagggcacat gctccgcgcc catctcttcc agcacttctg cggtatcgat atcagtaccg
    31501 atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc aatacacata
    31561 aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca
    31621 acggtgcatt cgctaccaag agtggcaaaa ttgcttaaat tcttcgccgc gccaaacccc
    31681 cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg acggatttca
    31741 ccgcgcgtta ttcgtgccgc ttcaatcagc acgttgcgcg tttccgtcat cgcttcgcca
    31801 gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac cgcctgcgca
    31861 ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga accgtcatag
    31921 acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct ttcgcaattg
    31981 actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta actgattcat
    32041 gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg
    32101 cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt gttggcgcag
    32161 tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca cccacgcttt
    32221 cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt caccgacgtt
    32281 atcttcccag gcaggaaggt caggagactg aatttgctga cccagctgtt gcagatggcg
    32341 taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa tgcctttttt
    32401 atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca tcttctcaaa
    32461 caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact gttccagcat
    32521 gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg tactctcctc
    32581 atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa cagaaagcgg
    32641 cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt tcagcacgtt
    32701 agcggttaag gccaccagcg gcggtaaatc ctctcgcgga taacgcttcg tcagttcacg
    32761 agagatatcc agcccggtca tatctggcaa ctgaatatcc agtaacacca ggtcgtattc
    32821 gcccggttta aacatctcta gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc
    32881 taatttttcc agcacagaac gcgcaacaat cacgttcagt tcaatgtctt ccaccagcag
    32941 cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc
    33001 taccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac
    33061 cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac caataccggt
    33121 gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg cgaaaatttt
    33181 atccagctca tcctgcggaa tgccgatacc agagtcttcc acttcaaaat gcagcatatc
    33241 gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt
    33301 actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg
    33361 taatggcagc gtcggctcca ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga
    33421 gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggttgattat caagctggac
    33481 cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc ccagcgtgac
    33541 ggccgaaaca tggatggtct tgagatattt ttcctgctcg gcggtgagtt cggtatccag
    33601 cagaatgcgg ctcagaccga cgataccgtt cagcggtgta cgcaattcgt gactgatggt
    33661 ggagataaac gtcgttttgt cgcggctggc ccgttcaagc gcatcctgat accgcttacg
    33721 ctcggtaatg tctcgaccaa agcccatcaa accgtgacgt ttacccacgc ggtcgtagta
    33781 cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca gccactgttc
    33841 ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga cttttgcggc
    33901 ggcttccggt gagtaaacat caacaggttt caggtgaacc agttgttttt cgctttttcc
    33961 ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt tacgataaaa
    34021 aaccaggtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct gctcgagctg
    34081 aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg tttcctgcag
    34141 ttctgcttcc gctttttcac gaacggcgat ttcctgattt agctgggcaa tattatcttt
    34201 taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt gcaccagccg
    34261 tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa agtagaccgc
    34321 ccacggcgta atcaacaaac caaagaagat agaacgaata acatcaatgc tttcgacctg
    34381 accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga cgagggccag
    34441 cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa cataatactg
    34501 cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc cccaataata
    34561 cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc ctcaacgacc
    34621 tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc atgttcattc
    34681 agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag aggagcaagc
    34741 agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg
    34801 cgaatcattt ctccggcagt gagcgtgtaa tcctccagtt caataccgtt caaacgcccc
    34861 gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcaacggatg tagctttata
    34921 ccgtcaacac cggtttccac tacccgctcc agcgtttgca gacattcagc cagcccttcg
    34981 cctggcagac cgacaattaa atgcgagcac accttcagcc cgcgctggcg tgccagctgc
    35041 gtcgtgcgct gataacaggc gaaatcatga ccgcgattaa tgcgatgtag cgttttgtcg
    35101 tgcacggttt gcagccccag ctccagccac acttcgtagc cctggtcttt atattcgcaa
    35161 agcagatcca gcaccgcatc cggcacgcag tccgggcgcg tgccgacaca caaaccgaca
    35221 atattggcct ggctcaccgc ctgttgatac atcgaacgca gcacctgaac ttcggcaaag
    35281 gtgctggtat acgcctggaa ataggcgagg taacgtttcg cgcgattaat taaattcgcc
    35341 tgatgcgcca gctgttcggc aatggaacga tgctgctgcg cttcgtcggc aaacgaggca
    35401 acattacaga aggtgcagcc gccgcgcccg atggtgccgt cgcgattcgg gcaactgaaa
    35461 ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag atcaccacca
    35521 aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg aaaagaggcc
    35581 aaagcctgcc atttttagct caattcggcg atgacctgga tcaatcgtcc cggcccgctt
    35641 ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac ataaatcact
    35701 tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa aaacagtagc
    35761 atgaaacgtc attaccaatt aatgcagtat aaaatgctgg ttttctcgtc aattcaaggc
    35821 aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc taacgacttc
    35881 ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa gaattttgcg
    35941 ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa
    36001 gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa
    36061 ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg
    36121 aggcgcgcgt atgacacgca acccccgtcg ccacgctctt tctgtacccg tgcgcagcgg
    36181 ttcggtagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc
    36241 ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca
    36301 caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct
    36361 cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt
    36421 tcgcatcgtt gcgcaggagc gcggctggcg tttagcgaaa aactacgccg tcgggatgct
    36481 attcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagaact
    36541 gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtact
    36601 gggtgaaatc gccctctcct ctctgccccg cattgagcaa atttttgtga acgccccggc
    36661 aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa
    36721 gcgtctcgaa gccgacaaag acttctacgt ctgtagccta tcgaatctgg tgaacatcta
    36781 taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg cggacctgcg
    36841 tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg
    36901 gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca ccatcactgg
    36961 taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctta tccctgatct
    37021 gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga tggataacat
    37081 gctggaactc ctgctggcag gcgggatgga tatcatccgc gccatgcgtc tgttagtgcc
    37141 acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa
    37201 ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtccg acggtcgttt
    37261 tgccgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca tcaccaaaga
    37321 taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt
    37381 cgaaaaaggc cgcgtcgggc caggcgaact gatggttatc gacacccgca gtgggcatat
    37441 tctgcactcg gcagaaaccg atgacgatct gaaaagccgc catccatata aagagtggat
    37501 ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag
    37561 ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga
    37621 agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat
    37681 gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt
    37741 ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt
    37801 tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca
    37861 ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac
    37921 gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg acgtcactaa
    37981 aaccacgctc gaagcgacag tcaaagagct gtgcgacaaa gccgaaaaaa tggtacgtag
    38041 cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc
    38101 agccccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga
    38161 tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct
    38221 gggcttcggc gcgacggcta tttatccata ccttgcctat gaaacgctgg gccgcctggt
    38281 agacacccat gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat
    38341 caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg
    38401 ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc tgtgcttcca
    38461 gggggcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg atctgctgaa
    38521 tctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc tgctgaaata
    38581 cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaagc
    38641 ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta atgagcgtcc
    38701 ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc
    38761 tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga tgtctatcgg
    38821 cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca tcggcggtaa
    38881 ctcaaactcc ggtgaaggcg gtgaagatcc ggcgcgctac ggcaccaaca aagtgtcgcg
    38941 catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg tcaatgccga
    39001 cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg
    39061 tgataaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag tgacgctgat
    39121 ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc tcattttcga
    39181 cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg aaccgggcgt
    39241 aggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca tcgcaggcta
    39301 tgacggtggc accggcgcaa gcccgctgtc gtcagtgaaa tacgcaggtt gcccgtggga
    39361 gctggggctt gttgaaaccc agcaggcgct ggttgccaat ggtctgcgtc ataagatccg
    39421 tttgcaggtc gatggcggcc tgaaaaccgg tgttgatatc atcaaagcgg cgattctcgg
    39481 cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta aatatctgcg
    39541 tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca aactgcgtaa
    39601 gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta tcgcccgtga
    39661 aacccgcgag ctgatggcac agctgggggt aacgcggctg gtggatctga ttggtcgcac
    39721 cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa
    39781 gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg aaaacaaccc
    39841 gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga
    39901 tgaacgccag agcaaaacct tctggttcga tatccgcaat accgatcgct ccgtgggcgc
    39961 gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatcctat
    40021 caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtatggaacg cgggcggcgt
    40081 ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt
    40141 aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa gtattatcgg
    40201 caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga
    40261 acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg gcgacaacgg
    40321 ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg ttaacttcgg
    40381 tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg
    40441 cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc atgaagagca
    40501 tctgcgcggt cttatcaccg agcatgttca gcatactggc tctcagcgcg gtgaagagat
    40561 tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga agtccagtga
    40621 tgtaaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg tgcaggcgca
    40681 gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga
    40741 tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt acgagccgtt
    40801 ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca acccatactg
    40861 cgagtggaaa tgtccggtac acaactacat cccgaactgg ctgaagctcg ccaacgaggg
    40921 gcgtattttt gaagcggcgg aactgtcgca ccagaccaac accctgccgg aagtttgcgg
    40981 acgagtctgc ccgcaagacc gtctgtgcga aggttcctgc actctgaacg atgagtttgg
    41041 cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg
    41101 gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta tcggcgcagg
    41161 cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt
    41221 cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct tcaagctgga
    41281 aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg aattcaaact
    41341 caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt acgatgccgt
    41401 cttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga
    41461 tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg
    41521 tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtgg tccttggcgg
    41581 tggcgacact gcgatggact gcgtgcgtac gtccgtgcgc cagggagcga agcacgttac
    41641 ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc
    41701 gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg aagtgaacgg
    41761 taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac cggatgccaa
    41821 aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg cagatgcagt
    41881 gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac acagcgtcga
    41941 gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct tccagaccag
    42001 caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg tggtaaccgc
    42061 tattgccgaa ggtcgtaagg cggcagacgg tattatgaac tggctggaag tttaagctct
    42121 gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa
    42181 gcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg ttaccggtag
    42241 atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg gacagataat
    42301 ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg gtattattca
    42361 cctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac
    42421 atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg caagggctgc
    42481 gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt ataagcgtgg
    42541 tttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga
    42601 aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga caccatctgc
    42661 agaattccca tcggcaaaga tttggcagac gctgtctgcc aaattgagag gctgtttgat
    42721 atttccacag cttttgtatt gcatctccta ttttcggttt gtatattcat gcaaataaac
    42781 gaaggaaagt tgccgtgatt tacattgaac agcttgaatt aatccataaa agtggtgatg
    42841 tgttgtatcc agtaaaaata accagaaaat catcaggaaa aactgccttc caccttgttc
    42901 cattcggctt aaataagact cacgaccttc tagaggtgga agatgcttca gaagcaattc
    42961 ggctggtgat tgatgaacgt cattccattc gctgcagtac acttacagca actattacga
    43021 ataaaaaggg caagcgaatt aagcggacag gtatatacag tatcaagggt gttaacataa
    43081 aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt ggctctgtgc
    43141 caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc agccttaacc
    43201 ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg cttatgcggc
    43261 tctcctagca taaacacggc gaacattccc ggtttcagga tgatggcttg ctcgttttca
    43321 atggtgctgc aaagctggta atcatcctca tggtggaact cttcacactg acgcgcagtg
    43381 cctgccatgc caaacagaat ccgttcttca ccgtttaata acagctggat atcaatgtat
    43441 tgctcgtgca gctccgcttt tttctcaacg ggcgattgag tgttaaacgt catgacattc
    43501 ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg cggtctggca
    43561 gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga cggtaatgac
    43621 cgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgctgcc ccaagtaaac
    43681 ctgcatcatg gcggtagtgc gccgccagta aatcaacatg aaataccgct ggctcctgcg
    43741 ccagatacgt ttccaccaac gccagatacc cttctgccag accaacgctg ccaccgacca
    43801 ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgcc agcacatgtg
    43861 cggagcggtg aatcagttgc tgcgcctgct cgtcgccctg cccggcgcgt gtgaaaatag
    43921 ttctcgcatc cgcgcccatg agttcacctt gcgctgccgc tgcaataccg cgaccagaag
    43981 caatcgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt ggatcggcaa
    44041 gcgtatgccc gatatgcccc gccagaccgc cagggccggt aagcagttta ccgccgctca
    44101 ctacaccgcc gccgacgcca gtggaaacgg tgataaacac catctcggtt acatcgcctt
    44161 ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca atggtcggca
    44221 aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca ccgagattat
    44281 gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc
    44341 gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc aaggcttctg
    44401 gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa
    44461 tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtgtggtc atagcaccgc
    44521 ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa tggcagaacc
    44581 gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag gcgtgttgta
    44641 acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca gcgccagatc
    44701 cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa taatttcggc
    44761 tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa
    44821 accgtgatga tgtatacgtg ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt
    44881 gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat cttcaatata
    44941 ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa taatcggcac
    45001 gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa tggcaaccgc
    45061 gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat cgagcgggct
    45121 gtcaggaatc ggctggcagg agacaatcag gccaccgtta gcagcgattt tttgatccag
    45181 ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata gaccgggcaa
    45241 caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg cacggcaccg
    45301 ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg cagccaacgc
    45361 tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt caggctgaac
    45421 gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc gccgatgatt
    45481 ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc cgctgcacgc
    45541 tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat cccttgtcca
    45601 agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc gccaatcgca
    45661 aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc tttgcgggtt
    45721 cccagccagt caccgaggaa gcctcctacg cagcatccca cggccgcgcc aaagccacta
    45781 aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt cagatacgtt
    45841 ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac caccatcagc
    45901 attacgcccg ttggccagcg tttgcctgta ctctgcacca taaagctgat aaagattgcg
    45961 gcgcataaca gcccaagaac agcgacgatc gcggcatttt gcaggttacc ggcgaagcag
    46021 aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg atgttcacca
    46081 cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt ctctttccag
    46141 tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg caaaatgccg
    46201 ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac ctgagcggca
    46261 acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt acgcagatgt
    46321 tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc catccccatg
    46381 ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc gcaggccagc
    46441 gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc gtagcggtca
    46501 cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc ggagatcaga
    46561 cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag taccagggcg
    46621 attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga aaatgcgcgc
    46681 cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt actcattgtg
    46741 agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg gtacagatgc
    46801 gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc cgcgctcttg
    46861 catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggtccaaa
    46921 cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag ttttcaggcc
    46981 gcggaatacg ccagttttga tcagtaaatc aatgactttg ttgcactcag tttgcagttt
    47041 ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat atcgccagcc
    47101 catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc cggaagcgaa
    47161 gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga tctgttccat
    47221 ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg taaccagggt
    47281 gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt acacaaccat
    47341 cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct
    47401 gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga
    47461 tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat
    47521 cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg aaaggctttg
    47581 tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct gctgaatatt
    47641 gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttggt caaaaggagt
    47701 caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg aagtgatact
    47761 tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc aacaccccat
    47821 acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt ttgttggtgg
    47881 tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc aacgcacgat
    47941 cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc tgttgatagc
    48001 taacgttgtt atgctcgtgc agagcctgat cagccacggt tggacgtgcg gcgatcagcc
    48061 agtcgagcag ggcaacgtgg atcgccatga agattgggtt accagggatc tccgccagca
    48121 cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc gactgactgt
    48181 tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt tcagccgcat
    48241 agcgcaccag actggattca aagaacagac gtaattgttc gaaatgggca atcccaccgg
    48301 gatgagaaag gaaatctttc gccatgccgg aaaactcacc gatgatagtg tccgcagaag
    48361 gacgcgagac gcgagcgcgt tcgccgttgt ttatttgcac cagacctttg cgttttaacg
    48421 ctgccagcgc ttcgcgcaca gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt
    48481 cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt tccagctctt
    48541 cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag ttgcgaccaa
    48601 ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc attctgtaag
    48661 gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta ggggaaaatg
    48721 gcaggttttt ctctttgtgc ctcatcatta ccataattaa cggaataatt aactattgcg
    48781 aaaaattaat gtaacgcaga taaacacatc ccgattgaat tattcataag actattcaca
    48841 agcattatga atattatgaa tgtgttctta caaaataatc ataagcgcat attttttaat
    48901 gaaaaatcac atcacctaca attaaaaaca cgacatccgc accataaata gacttgcaaa
    48961 aaatataaca tcattgtttt caatctgccg tttatgggat tgaccgtttt cttttgacac
    49021 ggagttcaac aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt
    49081 tgatcctgaa aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga
    49141 tgtgcggagt ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact
    49201 tgatcgtcga catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg
    49261 ggctgtcgat tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtc
    49321 gcgcgatggt gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc
    49381 tgtcggcaac ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc
    49441 tgggtatgtt gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc
    49501 tctctgcggt ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga
    49561 actccatttt tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc
    49621 agcttccggt cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac
    49681 gcgcttttga caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg
    49741 ataatgtccc gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg
    49801 gctcgctgtt cctcgcccac gtcgggctga tgaagtcaga actgcatctg gtggtggtga
    49861 tgttactaag tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg
    49921 aaacaatgga cgatgtgcag gcgttttttg acggcatggg tacgcagttt gccaacgtgg
    49981 taacgctggt ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg
    50041 atgcggttat caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta
    50101 tggcgctagt cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt
    50161 catttgccag tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga
    50221 ttatgccgat gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg
    50281 tgaagcggac agcgatcccc atggcagtcg gtttcgtggt gaatatgatt gccacaatca
    50341 cgctatttta ttaagtcatt aaaaagacaa aacaggccgc ctgggcctgt tttgtattac
    50401 ttcacaacac gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc atcgtcagga
    50461 tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa ccgacataac ggtttcgttg
    50521 tctgccgatg cctcttcatc attcatgatg ctggtatctt catcgtaggc agcttcaggc
    50581 tcaaacatcg tgcctgcgcc attttcacgg gcgtagatag ccagcacggc agccagcggc
    50641 acagaaacct gacgcggaat gccaccaaag cgcgcgttaa agcgcacctc atcattcgcc
    50701 agttccagat tgccgacagc acgcggcgca atgttgagta cgatttgccc gtcacgcgca
    50761 tattccatag gaacctgcac gccagggagc gtcacatcca ccaccaggtg cggcgtgagc
    50821 tggttatcca gcaaccactc atagaatgca cgcagcagat agggacgacg tggtgttagc
    50881 tgtgacaaat ccatacagat taactccggc ccagacgcat ttcacgttct gcttcagtta
    50941 aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat atagcctttc agctctttcg
    51001 cacccgggcc gctgagctcg atacccagtt gcggcagacg ccacagcagc ggagcaagat
    51061 agcaatcgac caggctgaac tcatcgctca ggaagtacgg cttctgaccg aagaccggcg
    51121 caatcgccag cagttcttcg cgcagttgct tacgtgcggc atctgcttca gaagctgaac
    51181 cgttgatgat ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc atgtacagac
    51241 ggctttcacc gcgagctacc gggtaaacag gcatcagtgg cggatgcggg aaacgctcat
    51301 ccagatattc cataatgatg cgagattccc acagggtcag ctcacgatcc accagggtcg
    51361 gaacgctctg attcgggttg aggtcaatca gatcctgagg cggattgtcc ttttccacgt
    51421 gttcgatctc gaaacttaca cctttctcag ccagcacaat gcggacctga tggctataga
    51481 tgtcagtagg accggaaaac agcgtcatta ccgaacgttt gttggcagcg acagccatga
    51541 aaacctccag gtatagtcag aatttttact gctaccagcc accaggtggc cagtcagaag
    51601 ttgtgttacc caataaggaa cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat
    51661 acccgacatt tgggcagaaa attggatgat agtttaccag attttgcgac cattgtggtg
    51721 agtggatgcc ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt tgacttagac
    51781 ccttattggc gatgtggttt ttgttttgcc tgtctttcag acggcagcaa aaagcaactt
    51841 cccatttttt acgctgattc agaatttagc cataaaaaaa cccgccgaag cgggtttttt
    51901 cgtaaattgt tttctgccgg agcagaagcc aattaacgtt tggagaactg cggacgacga
    51961 cgtgctttac gcagaccgac tttcttacgt tcaacctgac gagcgtcacg agtaacgaag
    52021 ccagctttac gcagttcaga acgcagggac tcgtcgtatt ccatcagagc gcgggtgata
    52081 ccgtgacgga tcgcaccagc ctgaccagag ataccaccac ctttaacggt gatgtacagg
    52141 tccagtttct caaccatgtc gaccagttcc agcggctgac gaactaccat gcgggcagtt
    52201 tcacgaccga agtactgttc cagagaacgt tggttgatta cgattttacc gttgcccggt
    52261 ttgatgaaaa cgcgagctgc ggaatttttg cggcgaccag tgccgtagta ttgattttca
    52321 gccattgcct ataatcccga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg
    52381 tgctcgttac ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt
    52441 ggcaacatgc ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct
    52501 tcaaagctca cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca
    52561 gtacgcttgt tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg
    52621 gtatctacgt gcggagtgta ttccgctttg tgcttaccgc gtaggcgacg agccagttca
    52681 gtagccagac ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg
    52741 gtttctggtt tagctgtaaa agttttcatt aaaagcttac ccaataaata gttacacgtt
    52801 ggtgaacacc caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac
    52861 cccttcgaat agcctatgcc agcacacaaa aagttttggg aaaaaaactt tcttgtaacg
    52921 tggggtcgca ggattataga gaagtcgggg tcaaagatcg accccttttt gtgatttgtg
    52981 acaggtttta acccgccaaa tgctcgcgct tcagatactc ttcgctttgc atctcttgca
    53041 gacgtgacag gcaacgctgg aactcaaact tcagccgatc gccctgataa atttcataca
    53101 gcggcacttc tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg
    53161 caataaagcg ccgcgcttcg ctctccatca accgcgtcat aactggtaca tcaaacaaca
    53221 tgaccgtatg aaagagacgt gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc
    53281 acagcgtagt aaaagagacc gccagcgtct ggttctcgac gcccattgtc gctaatggcc
    53341 gatggttgat ttctaacgtc ggtgaatttt ctcgtttccc ccccgccagc gccaaccata
    53401 gtttatccat ttgcgcccgg gtttcatcgt ggagtggcga aagccacaga tgcgcctgag
    53461 tgagtgtacg cagtcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt
    53521 taatggcatc gattgcaggc agaaaacgcg cacgttgcaa gccatttcga taaagttcgt
    53581 ccggcggaat atttgacgtc gctaccaggg taataccgcg ggcgaacaag gctttcatca
    53641 gaccgccaag tagcatggca tcggtaatat cagaaacaaa aaattcgtca aaacagagca
    53701 cgtcagtttc ggctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgta
    53761 aggcagttag ctcttcatgc acccgcagca taaaacggtg aaagtgcagg cgctgtttcc
    53821 gctctcccgg caggctttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac
    53881 cgccccacat atataagcca cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca
    53941 gcttaccgac ccgcgccatt agcccactcg tcctgggggc tggtggcgtg ctattgatga
    54001 gttcctgata aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat
    54061 ggctgccttc attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct
    54121 tattgttatt ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt
    54181 tctacactac gcgatatgca gtcgggattc cacttctgtg gaattaacgg ttatagtggc
    54241 ataatcagcc gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg
    54301 agatgttcat gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg
    54361 ctgtggccat gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac
    54421 tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc
    54481 gcagcgcgga attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg
    54541 cgaaaagttc cagcagcctg ctgccggaac tgtctgctga agcaaacccg ttccgtaatc
    54601 gtctggcaga gtctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt
    54661 ctgaaggcgc atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttcggg
    54721 cgcagccatt gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca
    54781 ttgttagccg tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt
    54841 caggtacgag agcaggaata atgaaaaaac aaccccagct gttgagtgca ttagcgttaa
    54901 gtgtcgggtt aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg
    54961 ccgatcaggc ccctctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg
    55021 tgagcgtacg ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa
    55081 agttttttgg tgatgattta ccggagcaac ctgcacaacc cttcgaaggt ttaggctccg
    55141 gtgtcatcat caacgccagt aaaggctatg tgctgaccaa caaccatgtg attaatcagg
    55201 cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta
    55261 gcgatgacca gagcgatatc gccctgttac aaattcaaaa cccgagcaaa ttaacgcaaa
    55321 tcgctattgc cgactccgat aaattgcgcg tcggtgattt tgccgtagcg gtcggtaacc
    55381 catttggcct tgggcaaacc gccacctctg gcattgtttc cgcattagga cgcagcgggt
    55441 tgaatcttga aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact
    55501 ccggcggtgc actgttaaac cttaacggtg agttaattgg catcaacact gcaatccttg
    55561 cgcctggcgg cgggagcgtc gggattggat ttgccatccc cagtaatatg gcgcgaacac
    55621 tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg tttgttaggc atcaaaggca
    55681 ccgagatgag tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg
    55741 tcagcgaagt gttgccaggt tctggctcgg cgaaagcggg cgtcaaagcg ggcgatatta
    55801 ttaccagcct caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcga
    55861 ccaccgagcc gggcacaaaa gtgaaacttg gcctgctgcg taacggcaaa ccactggaag
    55921 tagaagtgac gctcgatacc agcacctctt cgtcggccag cgctgaaatg atcgcgccag
    55981 cgctggaagg tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa ggtattaaga
    56041 tcgatgaagt tgtcaaagga agcccagctg ctcaggctgg cttgcaaaaa gacgatgtga
    56101 tcattggcgt caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg
    56161 caaaatcggc catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc tatctgctga
    56221 tgcgttaatg tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta actcgtggta
    56281 tgctgctgcc gttccctttt ttaatgacgc ctccatcatg tttgtgaagc tcttacgttc
    56341 cgttgcgatt ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag
    56401 ccttaacccg ctttccactc cgcaatttga cagtaccgat gagacgcctg ccagctataa
    56461 tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg gtttgaacac
    56521 caactctcac aaccagcttg agatccgcac cctgggatcc ggtgtaatca tggatcaacg
    56581 cggttatatc atcaccaata aacacgtcat caacgacgcc gatcagatca tcgtcgcctt
    56641 acaggatggt cgtgtatttg aagcattgct ggtgggatct gactctctaa ccgatctggc
    56701 ggtacttaaa attaatgcca ctggcggttt acctaccatt ccaatcaatg cacgtcgcgt
    56761 accgcatatt ggcgacgtgg tactggcgat cggtaacccg tacaacctcg ggcagaccat
    56821 tacccagggg attattagtg ccacgggtcg aattggtctg aacccgaccg ggcggcaaaa
    56881 cttcctgcaa accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc
    56941 gctgggcgaa ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac
    57001 gccggaaggt atcggctttg cgattccttt ccagttagca accaaaatta tggataagct
    57061 gatccgcgat ggtcgcgtga tccgcggcta cattggcatt ggcggacgtg agatcgcacc
    57121 gctgcacgca cagggcggtg gtatagatca actgcaaggg atcgtggtta atgaagtgtc
    57181 acctgacggc ccggcggcga atgcgggtat tcaggtcaac gatctgatta tttcggtgga
    57241 taacaaaccg gccatctctg ctctggagac gatggatcag gtggcggaaa ttcgccctgg
    57301 ttcggtgatc ccggttgtag tgatgcgtga tgataagcag ttaacgctgc aggtcaccat
    57361 tcaggaatat ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg gagtctgtg
//
LOCUS       DAJTVE010000009        55345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000009 DAJTVE010000000
VERSION     DAJTVE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO439_000538"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO439_000538"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6315000.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO439_000539"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO439_000539"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6315001.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO439_000540"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO439_000540"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6315002.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO439_000541"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO439_000541"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6315003.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO439_000542"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO439_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6315004.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO439_000543"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO439_000543"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6315005.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO439_000544"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO439_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6315006.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO439_000545"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO439_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6315007.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO439_000546"
     CDS             6537..6773
                     /locus_tag="OO439_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6315008.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO439_000547"
     CDS             6776..7633
                     /locus_tag="OO439_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6315009.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO439_000548"
     CDS             7654..9645
                     /locus_tag="OO439_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6315010.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO439_000549"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO439_000549"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6315011.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO439_000550"
     CDS             complement(10248..11144)
                     /locus_tag="OO439_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6315012.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO439_000551"
     CDS             complement(11193..11432)
                     /locus_tag="OO439_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6315013.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO439_000552"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO439_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6315014.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO439_000553"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO439_000553"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6315015.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO439_000554"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO439_000554"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6315016.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO439_000555"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO439_000555"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6315017.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO439_000556"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO439_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO439_000557"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO439_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6315018.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(19648..19800)
                     /locus_tag="OO439_000558"
     CDS             complement(19648..19800)
                     /locus_tag="OO439_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315019.1"
                     /translation="MLKNTFRWRAQKNAAVKAKNGYKYASLPASEKKRIFEMQQIEQR
                     DKIAKA"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OO439_000559"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OO439_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6315020.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OO439_000560"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OO439_000560"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6315021.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OO439_000561"
     CDS             complement(21570..22739)
                     /locus_tag="OO439_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315022.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO439_000562"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO439_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6315023.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OO439_000563"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OO439_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6315024.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OO439_000564"
     CDS             complement(24315..25247)
                     /locus_tag="OO439_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6315025.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OO439_000565"
     CDS             25360..26571
                     /locus_tag="OO439_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6315026.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OO439_000566"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OO439_000566"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6315027.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO439_000567"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO439_000567"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6315028.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OO439_000568"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OO439_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6315029.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OO439_000569"
     CDS             complement(30320..31576)
                     /locus_tag="OO439_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6315030.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OO439_000570"
     tRNA            31884..31960
                     /locus_tag="OO439_000570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OO439_000571"
     tRNA            31965..32041
                     /locus_tag="OO439_000571"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OO439_000572"
     CDS             32149..32454
                     /locus_tag="OO439_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6315031.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OO439_000573"
     CDS             32580..34184
                     /locus_tag="OO439_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6315032.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OO439_000574"
     CDS             complement(34196..35008)
                     /locus_tag="OO439_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315033.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OO439_000575"
     CDS             complement(35012..35797)
                     /locus_tag="OO439_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6315034.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OO439_000576"
     CDS             complement(35794..36462)
                     /locus_tag="OO439_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6315035.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OO439_000577"
     CDS             complement(36526..37164)
                     /locus_tag="OO439_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6315036.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="OO439_000578"
     CDS             complement(37177..39285)
                     /locus_tag="OO439_000578"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6315037.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="OO439_000579"
     CDS             complement(39306..39932)
                     /locus_tag="OO439_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6315038.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO439_000580"
     CDS             complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO439_000580"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6315039.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41154..42566
                     /gene="pykF"
                     /locus_tag="OO439_000581"
     CDS             41154..42566
                     /gene="pykF"
                     /locus_tag="OO439_000581"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6315040.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42876..43112
                     /gene="lpp"
                     /locus_tag="OO439_000582"
     CDS             42876..43112
                     /gene="lpp"
                     /locus_tag="OO439_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6315041.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO439_000583"
     CDS             complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO439_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6315042.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO439_000584"
     CDS             complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO439_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6315043.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO439_000585"
     CDS             complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO439_000585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6315044.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO439_000586"
     CDS             complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO439_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6315045.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO439_000587"
     CDS             complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO439_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6315046.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO439_000588"
     CDS             complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO439_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6315047.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO439_000589"
     CDS             complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO439_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6315048.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50388..50576)
                     /locus_tag="OO439_000590"
     CDS             complement(50388..50576)
                     /locus_tag="OO439_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6315049.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO439_000591"
     CDS             complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO439_000591"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6315050.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51083..54139)
                     /locus_tag="OO439_000592"
     CDS             complement(51083..54139)
                     /locus_tag="OO439_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6315051.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54528..>55345
                     /gene="ydiK"
                     /locus_tag="OO439_000593"
     CDS             54528..>55345
                     /gene="ydiK"
                     /locus_tag="OO439_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6315052.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTG"
BASE COUNT        13346 a        14560 c        13534 g        13905 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattatcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgcagaaaat aaaaataata
    40261 ttatcgttat agattaaaat acataccgca attcgtataa tttcagcagt tgttatttag
    40321 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    40381 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    40441 tgcgctgaat gcgtttattg gctaatgcag tacgtaatac gcctgcgata acaatatgct
    40501 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    40561 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat ttatcatgcc
    40621 aactatcagc atatatcaat ctaaccaatt aaacaaaaat caaacaaaat cagacaaata
    40681 acgcgataaa ttattttaac tgtagcaatt gagcgatgat atatttatac accggatgaa
    40741 ctttcactta tcctcacact gacaacttcg gcaccagacg ttgcgcaaac agcgaagttt
    40801 ttgcgtaacc ttttccctgg aacgttaaat ctttgataac aatttattgt ctaacaagtt
    40861 gtatattttt tgaaacgctg tttttgtttt ccttttggat taatttcagc gtataatgcg
    40921 cgccaattga ctcttgaatg gtttcagcac tttggactgt agaactcaac gactcaaaaa
    40981 caggcactca cgttgggctg agacacaagc acacattcct ctgcacgctt tttcgatgtc
    41041 acctatcctt agagcaaggc accaccactt tcgtaatacc ggattcgctt tccggcagtg
    41101 cgcccagaaa gcaagtttct cccatccttc tcaacttaaa gactaagact gtcatgaaaa
    41161 agaccaaaat tgtttgcacc atcggaccga aaaccgaatc tgaagagatg ttagctaaaa
    41221 tgctggacgc tggcatgaac gttatgcgtc tgaacttctc tcatggtgac tatgcagaac
    41281 acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta
    41341 tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg
    41401 tttctctgaa agcgggtcag acctttactt tcaccactga taaatctgtt atcggcaaca
    41461 gcgaaatggt tgcggtaacg tatgaaggtt tcactactga cctgtctgtt ggcaacaccg
    41521 tactggttga cgatggtctg atcggtatgg aagttactgc cattgaaggt aacaaagtta
    41581 tctgtaaagt gctgaacaac ggcgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg
    41641 tttccattgc cctgccagca ctggctgaaa aagacaaaca ggacctgatc tttggttgcg
    41701 aacaaggcgt agactttgtt gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa
    41761 tccgtgagca cctgaaagcg cacggcggcg aaaacatcca catcatctcc aaaatcgaaa
    41821 accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg
    41881 cgcgtggcga cctgggtgta gaaattccgg tagaagaagt tatcttcgcc cagaagatga
    41941 tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac tgcgacccag atgctggatt
    42001 ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg tgacgttgca aacgccatcc
    42061 tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg
    42121 aagcggtttc tatcatggcg accatctgcg aacgtaccga ccgcgtgatg aacagccgtc
    42181 tcgagttcaa caatgacaac cgtaaactgc gcattaccga agcggtatgc cgtggtgccg
    42241 ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt tgctacccag ggcggtaaat
    42301 ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat actggcactg accaccaacg
    42361 aaaaaacggc tcatcagttg gtactgagca aaggcgttgt gccgcagctg gttaaagaga
    42421 tcacttctac tgatgatttc taccgtctgg gtaaagaact ggctctgcag agcggtctgg
    42481 cacacaaagg tgacgtcgta gttatggttt ctggtgcact ggtaccgagc ggcactacta
    42541 acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga attaatttgt atatcgaagc
    42601 gccctgatgg gcgctttttt atttaatcga taaccagaag caataaaaaa tcaaatcgga
    42661 tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc tgttttctct
    42721 cagttttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat caaaaataag
    42781 tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
    42841 acatggagat taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg
    42901 gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc
    42961 agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga
    43021 acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc
    43081 tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca
    43141 cattgtgcgc catttttttg tctgctattt accactactg cgtcgcgcgt aacatattcc
    43201 cttgctctgg ttcaccattc tgcgctgact ctactgaagg cgcattgctg gctgcgggag
    43261 ttgctccact gctcaccgaa accggatacc ctgcccgacg atacaacgct ttatcgacta
    43321 acttctgatc tacagcctta ttgtctttaa attgcgtaaa gcctgctggc agtgtgtatg
    43381 gcattgtctg aacgttttgc tgttcttctg ctgatagtgg tcgatgtact tcaacataac
    43441 gcatcccgtt aggttccacg gaatatttca ccggttcgtt gatcactttc accggcgttc
    43501 ccgtccgcac gctggagaac aaggctttaa tatccggtgc attcatgcga atacaccctg
    43561 aactgacgcg caaaccaacg ctgtccggcg cactggtgcc atgaatgagg tattcgccat
    43621 taccatgcgc gaggcgcagt gcgtaacgtc ctagcgggtt atttggtccg gcaggaacga
    43681 ctggcggtaa tttaatgcca cgctccagcg aacgctgacg aatgcctgcc gtaggcgtcc
    43741 aggttgggtt agggattttc tgcccaacac gcgtttccat caccggcgtt tccagcccct
    43801 gcaatccaat acctattgga taaacctgca caatattttc tcccggcgga taataataaa
    43861 ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg tgcatcaggt aataacagtt
    43921 gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg gacgatagtg ttattggctt
    43981 caaggatcaa cattgccgca gtatcaaaac gtcgggcaat agcctgaagg tttttatccc
    44041 cttcttgcac cgtatacgtt tgattttgcc caaccagtcg gcttccggtt ggtggtagcg
    44101 gataatcaac cgcccaggca gcctggatgg cgctaaaagc gccgataagc gtgagtgtaa
    44161 gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc ggagactcac gctgaaacgt
    44221 cggatggcgc ttatgttcac ctgaaaccaa aacactcctg tgcaggtcag tgtaaacatt
    44281 gaccatccgg caatgtgagc caaccggatg aaagctgtcc ctttagttta gctaagtgca
    44341 gcggctttgg cgcgaattgc gcgaatcatc gcttccagac cttgtgaacg agatggggtg
    44401 agatgttggg tgagtgccat tttttcaaac cacggacgca catcgaaact gacaatatcc
    44461 tgcggcgtca tctgatcgta gagaataaag actaccgcaa taagcccttt cacaatcgcc
    44521 gcatcgctgt cgccctgcaa ttcaataatt ccgtgcgcat tctggcgcat gacaatccac
    44581 acctgactct ggcagccctg aatgctattt tgtgggcttc ggtcttcgtc gcgtaattca
    44641 gtcagacgct ggcccagctc aataatgtag agatatttct cttcccagtt ggcgcagcgt
    44701 aaaaaattac gcagcaattt ttctttatcc ggcaataaag ccatagtgcc tccctgttat
    44761 cccagcaaac ggtgaatacg ttgcaggccg gtcaccagac gatccacttc ttcatgggtg
    44821 ttatacatgg ccagcgacgc ccgacacatc gcagggacgt tgtaataggc cattaatggc
    44881 atagcgcagt gatgtccggt acgcacagca atgccgtaat tatcgagaaa actgccaaca
    44941 tcataggcgt ggtgtttacc gagattaaaa gcaataacgc caagcctgtt ttgtgggcca
    45001 tagagagtga gatccggtac agattccagc tgtgatagcg cataatgcat cagattctgt
    45061 tcatactcgg ctatgttatt aagccccagc gccgaaacat actccagcgc cgcgccaaga
    45121 ccaataatgc ccccggtatt gggtgtaccg gcttcaaacc gccatggtgc tttggtccag
    45181 gtagtgcctt cactcaggct gacggtggcg atcatagaac cgcccccttc ccacggcggc
    45241 atctcctgca acaaggcttc tttcacataa agaatgccaa ttccggtggg gccatacagt
    45301 ttatgcccgg agaacacgta aaagtcgcaa tccagcgcct gaacatccac cggatgatgc
    45361 atcaccgcct gagcgccatc caccagcact tttgcgccat gctggtgcgc aagcgtgatt
    45421 atttccgcca gcggattttc tgttcccagc acgttggaga catgagtaat tgccagcagg
    45481 cgagttttct catcaaacag cgtaggcagc gtctccagtt gcagcgtacc gtcggggttg
    45541 agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa gcatctgcca gggaacaatg
    45601 ttagcgtggt gctccatctg actgatgatg atgttatcgc ccgcccgcac gttgctgttg
    45661 ccccaactat tggcgaccag attgatccct tccgtcgtgc cgcggacgaa caccagctct
    45721 tccgccgaac gggcattaat aaacagcgat gcccgcttgc gcacgttctc cattttctcg
    45781 gtcgcctggg cgcttaaggt atgaataccg cgatgcaccg ccgcgtagcc gtgacgataa
    45841 aactcagcct cggcgtcaat cacctggctt ggtttctgcg cactggcggc gctatcgaga
    45901 taagccagcg gcaaaccgtt cacctcacga gaaagcaccg gaaagtcggc ccgcactttg
    45961 tcgacggaaa aagtcatctt gcacctcctg gcagccgttg accgattcgg gccagcacct
    46021 gctgtttaag cccctcatca cgcagtgctt ccgtcagttc ggcagcaaag gcgtaaatga
    46081 tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg cagatagaac atctgttcat
    46141 catcaatacg ccccaccgtc gcgccgtggc tgcatttcac atcatctgca tagatttcca
    46201 gctgcggttt tgtatccact tccgccattt tgcccatcag cagattattg ttggtcatct
    46261 gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa accgttaaat accgcgcggc
    46321 ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt acaaaaacct ttattgtgtt
    46381 ccagccaggt acgggtatca cacacctcgt ttttcaccgg catcgccagg ctattgatcc
    46441 gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt gtgtcgtaac actgcgccac
    46501 ccagcaggaa actatggcta aatgcggtgg catcctcagc cagcaacaaa tcgttatgag
    46561 caaagtggtg actgagcggg ttttcaaacg ccagcttgat atgctgcaag tgggcgttcg
    46621 ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg agcatcatta aggctgacaa
    46681 aatgttcgat caccgttgct tcggcacctt ccgccagatc cagatgatgc cggtaatggg
    46741 cagtgttcac ctcttcacct gccacgccct gggtgatatg cattaacagc aatggctttg
    46801 ccggccgttg accgcgcttc acggcgatat gcgtcacgct ttgtgccagg ctttccgtca
    46861 aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc ctgacggtcg tcgttaatgc
    46921 tcacttcata tccgctgcct tcaattgcat cgctcagcgc gggcacgtaa cgtccatcga
    46981 caaacaccag ccgcacggcg tctaacgtta acgctaaggc atcacgctgc tgtggggata
    47041 tatctcccgc aatgctgaca aactgactat tggtcagccc ttccagcggc gtatatttcc
    47101 agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa ttgttgtaaa tgctgctgtg
    47161 cttgcggtga gcgttttgtc ccttcagctt caaacaagtg atgccactgt tgcagcgcat
    47221 tactgctgtt cggtaagcca gccataaccc tgctcctcca gttgtttgac caacgtgaaa
    47281 tcgccggatt tcacaattcg tccctgatat agcacatgga cgtaatcagg cttgatgtag
    47341 tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc gcttgccgtc acgcagcgag
    47401 ttcacgccat cggcaaccac ttttaatgcg tcaatatcca gcccggagtc cgattcatca
    47461 agaatgcata actccggttc cagcaccgcc atttgcaaaa tatcgttgcg ctttttctcg
    47521 ccgccggaaa aaccaacgtt taccgaacgg gttaataaat cttccggcat cttcaggaga
    47581 gcgattttct cttccatcaa atcctgaaaa tcaaagcggt cgagcgtttc ctggccgcga
    47641 tagctgcgca ccgcattaag tgccgtttgc aggaaaaact ggttactgac gccgggaatc
    47701 tccaccggat actggaaggc cataaagatg ccttcgcctg cacgatcttc cggcgacagc
    47761 gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg tcacttcata atcttctcgc
    47821 ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt ttggccccat aatggcgtga
    47881 acttcgccgg gacgaacgtc gaggcttaat ccacgcagga tagctttatc ttccacgctg
    47941 acgtgtaaat ctttaatact taacatgttt attccttatc cgacgctgtg ttcaagactg
    48001 atggcgagga gtttttgtgc ttcaacggca aattccagcg gcagttctga gaacacgtct
    48061 ttgcagaaac cgttaacaat catcgagatg gcgtcttctt cgctaatccc gcgttgcagg
    48121 cagtaaaaca gttgatcttc accaatacgt gatgtcgttg cctcgtgctc cagttgtgcg
    48181 ctgttattgc gacattcgac atacgggaag gtatgcgccc cacaattagc gccaatcagc
    48241 attgagtcgc actgagtgaa attgcgcgca ttggttgccg tcggcatgat tttcactaag
    48301 ccgcgataac tgttctgact atgtccggca gagatccctt tcgagataat ggtcgatttg
    48361 gtgtttttac cgatgtggat catcttggtg ccggtatccg cttgctgatg accgctggtc
    48421 agcgccactg agtaaaactc accaatggag ttatcgccgc gcaaaatgca gctgggatat
    48481 ttccacgtaa tcgctgaccc ggtttctgat tgcgtccatg acattttgct gttttcgcct
    48541 tcgcacaaag cacgcttggt gacgaaattg agaataccgc cggtgttgtt atcgccaggg
    48601 aaccagtttt gtaccgtgga atatttcacc tcggcgtttt tatggatgat gacttccacc
    48661 accgccgcgt gtaactgata gctgtcacgc accggagcgg aacagccttc aatgtagctg
    48721 acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa actgcccggt tttttccgcg
    48781 ttaatgcgaa aataggtgga aagttccatc gggcagcgca cgcctttagg cacataaata
    48841 aacgtaccgt cagaggctac cgccgcatta agcgcggcaa agaagttgtc atttcccggc
    48901 accacagtgc cgagatattt acgcaccagt tccggatgat cgtggatcgc ctcaccaaag
    48961 gaacagaaaa taattccctg ctccgccagt ttttcgcgat aagtagtggc aaccgaaaca
    49021 gagtcgaaaa tggcatccac cgccacctct ttgccttccc gcacgggaac gcccaactgc
    49081 tcaaacgccg cctccacctc tttacttaaa aaggcgttcg cgccagtttg ctgcaccgcg
    49141 ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg atggtgctga gtagtaactg
    49201 taatcctgat aattcagctt gtcgtagtgc gctttcaacc agtgcggttc ttccatctcc
    49261 agccatgcgc gataggcgtt tagacgaaac tccagcatcc actccggctc attacgcttc
    49321 gccgaaattg cgcgcaccac ctcttcgttt atcccctttg ccagctcatc ggtggctaac
    49381 tgggtgaaga atccttcttt ataattcagc gggccgccgg tccaggtttt gacatcgtca
    49441 gttgcttcag tattacgaga catagtaccg cctatacccc aaagctttcg ccacagccac
    49501 attcattctg ggctttaggg ttgtgaaatt tgaatatctg attaagtcct tcacgaacga
    49561 aatcgacttc cgtgccatca ataaacggca tcgcttgcag cgggacaaac agcttcgcgc
    49621 cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct aacactgtcg agcacatagc
    49681 caaagcccgc gcagcccgtt tgcttcacgc ctaagcgcac gccgaccata cccggctgct
    49741 ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt cagcgttaag ccttgccagg
    49801 cgaaatcttg tgggttaaag gttcctgaat gcatgtccat cgatttacct cacttcatcg
    49861 ctttcagcgt ataacagcat gctagtgata atgattatca gttcaaccca gcaaacgcag
    49921 gggctttacc gtaaaacatg ctttttgcct gcttttaata agcatagacc ctgatgtgtg
    49981 ggttaacagg cacgctaaga attaggtatc tcattgttag ataatggtta ttatctaagg
    50041 agcattaagg ctgtgatgaa aaagaaaaag ttgtattgaa aatgactatt taagagatag
    50101 gtaaaaaagt gcagcgttca gaaataagaa aacccttaag tctgtgcgac acaggcttaa
    50161 gggtttctac cccatccggc gcttatctcc ggcactctca gtggcttagc tcttgaaggg
    50221 gcgataagaa taatctcata aagctaaccc gccgttttaa cacaaactgc gattagtatt
    50281 atttttgaac aatatcaggc ggtagataag cagtattaag aaggtcatcg aacctggacg
    50341 gaggttaatc caggtcgatt tggcgaactt gcggcattaa gtcaggatca atgcacgccc
    50401 agacgccagg caaagtagat ttcttctttt aattcagtag aagagagagt aagcaggtca
    50461 gcaaattcaa gttctagttg tttcagacgt ttgagatatt gggcaggtga aagattgctc
    50521 tggtcacggc gtaaaaattc aatggccagc tgggtgggat caagttgagt agacatagca
    50581 tcctcgcttt tagacaagac gtgcacagta taccaccgtt tactgtgcag ataatgacca
    50641 aaagcaatat gcgtcacact tttctggtga caacgtcaca aaatggcggt cgtcaatcgt
    50701 gacgaacagc acaaacgccc tttctcatcg aagatttcaa tctgccagac ctggtgacgc
    50761 gaaccgagat gcaacggttt acatatgccg cgcacccgcc cttctcgtgc cgagcggacg
    50821 tgattagcat tgatttccag accaaccact ttttgctcac cttcggtaca taaataaccg
    50881 gcaacggaac cgatactttc ggccagtacc acggatgctc ctccatgcag caacccgaaa
    50941 ggctgctttg tccgcgagtc tactggcatt gtcgcttcaa gggtgtcatc accaatatgt
    51001 tcaaagcgaa tatccaggaa ccccaccatg tttccttcac ccatagcatt cagtgcttcc
    51061 agggtgattt tccgtttcca tatcatttaa taatctccag taaagcctgc acaggatggc
    51121 gtacccccgt gccttcaacc cgttttacct ggctacggca ggaatatccg gtcgccagac
    51181 agcggttacg cggcagtcgc tgcatagcct gatgccagga taactcatag atcccgagcg
    51241 aattttcatg gtttttcgct tcatgtccgt aagtccctgc catgccgcag caacccacgc
    51301 tgacattttc cagtttcgcg ccaaaacggg caaatatcgc ggcccattgt gctggcgcgc
    51361 ccggcaaagc ggtaacctcg gtacagtgac caaagaaata ccatgattca ccgctgactg
    51421 tagccactgg atgtgactca agtgcgcttg ccagccattc attcgcaagt aagacgttaa
    51481 actcgccacg ttcctcgccc agcgccagtt tatattcatc gcgataacaa agtaccagcg
    51541 ccggatcgac gcccaccatt ggcatcccaa gcttagccat acggttgagg aaatccgccg
    51601 tctttttcgc cgtcttcgca aaacgattaa gaaagccttt gatatgctgg gctttgccat
    51661 ttggcgaaaa tggcagtaac acaggctgga aacctaattt ttcaaccaga cggacaaaat
    51721 ccgccaccac ttgcgcatcg taatagctgg taaaggggtc ctgcaccacc aacaccgtgc
    51781 gcgctttctg ctctgcattg agagcttcaa gctgttccag cgtcatgttt gccgagcgat
    51841 gccccaccat ttgttgttgt agcgagggga ccgacagcag tggcaaatca accatgccga
    51901 tatgtttttc cgagagtttg cgcaccagcg gctggttaat gaagaagtta aaggttttcg
    51961 gcgcgcgtgc catcagcggt gcgtagctct cgaccgtagc gacgaggtgg tcgcgcagcg
    52021 ggcgtaaata acgggtgtga tagagctgca ggaaacgaga gcgaaactcc ggcacatcaa
    52081 ttttgatggg gcactgggtc gaacacgctt tacaggccag acagcccgac atcgcctctt
    52141 tgacttcgtg tgagaagtca tattcgcctt tattcgcatg ccagctattg cgcgtgcggg
    52201 caattaacgt ccgtaaactg acgccggatt caggcagttc ttgttccagt ttgagtggat
    52261 caacgccgcg atccgccaac aaacgcagcc attcacgcac cagcgttgcg cgccctttcg
    52321 gtgaatgaat ccggttctgg gtgatcttca tcgacggaca catcggacta cgggcatcaa
    52381 agttgaagca taaaccgttg ccgttacact ccatcgcacc gcgccactgc tggcgtaccg
    52441 caatggggat ctgccgatcg aatgtaccgc gcttcaccgc gtccactttc atcatcggcg
    52501 catcgagacc ttctggcggg caaatcttcc ctgggttgag tcggttatgc gggtcaaatg
    52561 ccgctttcac tttgcgcagt tctgcaaaaa gttcctcacc gaaaaacgcc gggctgtatt
    52621 cagcgcgaaa acctttgccg tgctcgcccc acaacaaacc accgtatttc gcagtcagcg
    52681 ccaccacgtc atcagagatt tgcttcatca aaatctcttg ttgcggatcg cacatatcca
    52741 gtgccggacg gacgtgcaaa acacctgcgt cgacgtgacc gaacataccg tagcttaagc
    52801 cgtggctgtc gagcagcgcg cgaaattcag caatataatc ggccagatgt tccggcggta
    52861 cgcaggtatc ctcagcaaac ggaattggct tagcggcacc tttggcattg ccaagcagac
    52921 caacggcttt tttgcgcatt gcatagatac gttcaacccc cgccagctcg cggcacacct
    52981 gccagccaat cacacctgcc tgttggctgg caatcagctc atcaagccgc acacagagtg
    53041 cattaactcg ctcgtcaatc agcgcctcat catcaccagc aaattccaca atgttcagcc
    53101 cgagcatctc tttgtcaggc acatcggtaa tcaactcgct gacggaatgc cagacaatat
    53161 cttcccgcgc cagattcagc acttttgagt ccaccgtctc taccgaaagc gcccgcgcct
    53221 caaccataaa cggcgcgtta cgcagcgcgg agtcaaaaga gtcatatttg acgttcacca
    53281 gacggcgcac tttaggcaag ggcgtaatat ccagccgcgc ttcggtaata aaggccagcg
    53341 tcccttctga acccgtcaga atgcgcgtca ggtcgaactc ggtcatctca tcgttaaaga
    53401 catgacgcag atcgtaaccg gtaagaaagc ggttaagttt ggggaagttg tcgatgatta
    53461 actggcgttg cagacggcaa cgttgataaa ccgtgttata aattcgcccg attgtggtat
    53521 tggttttacc cagcgtttcc gccagttcga cgggtaaagg ttgcgtatcg agaatatcgc
    53581 cccccaacaa caccgcgcgt acgccaagta cgtgatctga cgttttgcca tagaccagcg
    53641 atccctgacc ggatgcatcg gtattgatca tcccgccgag cgttgcccgg ttgctggtcg
    53701 aaagttctgg tgcaaaaaag tagccgaacg gtttcaggta ctgattgagt tgatctttta
    53761 tcaccccggc ctcaacgcgc acccagccct cttcagggtt aatttcgatg atgcggttca
    53821 tatggcggga catatcaaca ataatcccct ggttgagcgc ctgaccgtta gtgccggtgc
    53881 cgccgccgcg gggggtaaag atcagcgatg aatagcgttc ctgcgcggca agacgggcga
    53941 tcagcgccac atctgcggtt gaacgcggaa ataccaccgc atcggggaga agttggtaaa
    54001 tactgttgtc ggtcgacatt gtcagacgat cggcataact tgtcgccgta tcgccggtaa
    54061 aaccttgttg ctccagctct tgcaaaaaat taagcaccag ttgaacgacg ccgggtgcct
    54121 gggaaatctg tggaatcatt atattgaccc tttcctgcgg tctgtgatgt aggtcgatac
    54181 actattcttt caggcggctg caatagcgca ctgaaaggtg atgtttattt actctatgga
    54241 tttcgagttg caggaaggcg gcaagcgagt gaactccagg agcttacaat agtaagtgac
    54301 tggggtgaac gaacgtagcc gcagcgcatg caacttgaaa tacgacgagt aaatcgtttg
    54361 cgtgttgcct gagttgttgt accacatttt tttctaacac gcccatcaga attaagggca
    54421 gaatcggcct gttaaaaacc gctgaaattg ctcatcatta tgcaggtgag tttcgcgtgt
    54481 tcacgtcgcg tcgacgattt gacgcacaaa aaaggtgaaa agtagttatg gtaaatgttc
    54541 gtcagcccag ggatgtcgca caaattctgc tttcggtgct gtttttagcc atcatgattg
    54601 tggcatgtct gtggattgtt caacccttta ttctcggctt tgcatgggcc ggtacggtgg
    54661 ttatcgccac ctggccggta ttgttacgtt tgcaaaagat catgtttggc cgccgctctc
    54721 tcgccgttct ggtgatgacg ctgttattag tgatggtgtt tatcatccct attgctttgc
    54781 tggttaacag tatcgtcgac ggcagcggcc cgctaattaa agccatttcc agcggcgaca
    54841 tgacgttacc cgatctggcg tggcttaata ccattccggt gattggcgcg aagctgtatg
    54901 caggctggca caacttgctg gatatggggg gtacggcgat catggcgaaa gtccgccctt
    54961 atattggcac caccaccacc tggttcgttg ggcaggcggc gcatatcggg cgctttatgg
    55021 tgcattgtgc gctgatgctt ctcttcagtg ccctgctgta ctggcgcggt gaacaggtgg
    55081 cacaaggcat tcgccatttt gcaacccgtc tggcaggcgt tcgcggagat gccgccgtgc
    55141 tgctggcggc acaggctatc cgcgcggtgg cgctgggtgt ggtggtgacg gcgttagtac
    55201 aggcagtgct tggcagtatc ggcctcgccg tatccggcgt accttatgca acgttgctaa
    55261 cggtgttaat gatcctctcc tgccttgtcc agcttggccc gttgccggta ctgattccgg
    55321 cgattatctg gctctactgg accgg
//
LOCUS       DAJTVE010000010        50054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000010 DAJTVE010000000
VERSION     DAJTVE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..1274
                     /locus_tag="OO439_000594"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OO439_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OO439_000595"
     CDS             1247..1876
                     /locus_tag="OO439_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6315053.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO439_000596"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO439_000596"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6315054.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="OO439_000597"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="OO439_000597"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6315055.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="OO439_000598"
     CDS             complement(4244..4441)
                     /locus_tag="OO439_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6315056.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO439_000599"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO439_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6315057.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO439_000600"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO439_000600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6315058.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO439_000601"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO439_000601"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6315059.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO439_000602"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO439_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6315060.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO439_000603"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO439_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6315061.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO439_000604"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO439_000604"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6315062.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="OO439_000605"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="OO439_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6315063.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO439_000606"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO439_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6315064.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="OO439_000607"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="OO439_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6315065.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="OO439_000608"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="OO439_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6315066.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="OO439_000609"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="OO439_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6315067.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO439_000610"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO439_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6315068.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO439_000611"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO439_000611"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6315069.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO439_000612"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO439_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6315070.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO439_000613"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO439_000613"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6315071.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24009..24209
                     /locus_tag="OO439_000614"
                     /pseudo
     CDS             24009..24209
                     /locus_tag="OO439_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="OO439_000615"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="OO439_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6315072.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYADETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="OO439_000616"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="OO439_000616"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6315073.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO439_000617"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO439_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6315074.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO439_000618"
     CDS             complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO439_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6315075.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28466..29584
                     /locus_tag="OO439_000619"
     CDS             28466..29584
                     /locus_tag="OO439_000619"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6315076.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29650..29898
                     /locus_tag="OO439_000620"
                     /pseudo
     CDS             29650..29898
                     /locus_tag="OO439_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            29963..30331
                     /locus_tag="OO439_000621"
     CDS             29963..30331
                     /locus_tag="OO439_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6315077.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30425..31078
                     /gene="nfsB"
                     /locus_tag="OO439_000622"
     CDS             30425..31078
                     /gene="nfsB"
                     /locus_tag="OO439_000622"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6315078.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31186..32433
                     /gene="ybdG"
                     /locus_tag="OO439_000623"
     CDS             31186..32433
                     /gene="ybdG"
                     /locus_tag="OO439_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6315079.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO439_000624"
     CDS             complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO439_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6315080.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO439_000625"
     CDS             complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO439_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6315081.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO439_000626"
     CDS             complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO439_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6315082.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO439_000627"
     CDS             complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO439_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6315083.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO439_000628"
     CDS             complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO439_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6315084.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40268..40951
                     /gene="cusR"
                     /locus_tag="OO439_000629"
     CDS             40268..40951
                     /gene="cusR"
                     /locus_tag="OO439_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6315085.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40941..42389
                     /gene="cusS"
                     /locus_tag="OO439_000630"
     CDS             40941..42389
                     /gene="cusS"
                     /locus_tag="OO439_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6315086.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            43126..45027
                     /locus_tag="OO439_000631"
     CDS             43126..45027
                     /locus_tag="OO439_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6315087.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            45055..45516
                     /locus_tag="OO439_000632"
     CDS             45055..45516
                     /locus_tag="OO439_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6315088.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            45536..49947
                     /locus_tag="OO439_000633"
                     /pseudo
     CDS             45536..49947
                     /locus_tag="OO439_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        12014 a        13762 c        13089 g        11189 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacacta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat gccgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtggcagc cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28141 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa
    28201 atggctgttt atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac
    28261 tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag
    28321 ctctgccata acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa
    28381 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28441 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28501 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28561 ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca
    28621 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28681 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28741 ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28801 taacgagcgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28861 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28921 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    28981 aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29041 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag
    29101 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29161 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29221 tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca
    29281 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    29341 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt
    29401 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    29461 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29521 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga
    29581 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29641 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29701 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29761 ctggtgacca tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg
    29821 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29881 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    29941 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30001 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30061 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30121 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30181 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30241 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30301 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30361 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30421 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30481 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30541 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30601 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30661 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30721 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    30781 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30841 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct
    30901 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    30961 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31021 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31081 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31141 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31201 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31261 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31321 cggaccttcg aaaaacgagc catcgccagt tcacggcttt ggttgcaaat cataacccag
    31381 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31441 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31501 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31561 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31621 ctgatcggcg cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31681 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31741 ccgattcttg gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc
    31801 gactggctgg agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc
    31861 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31921 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31981 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32041 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    32101 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    32161 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32221 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32281 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32341 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32401 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32461 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32521 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32581 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc
    32641 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32701 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32761 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32821 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32881 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32941 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33001 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33061 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33121 tgcgatacac cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag
    33181 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33241 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33301 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33361 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33421 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33481 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33541 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33601 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
    33661 ggcgcataat caggaaagcg atgatcccgg cgacgccgta acccagcaat acagccggac
    33721 ccgccatctg aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33781 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33841 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33901 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33961 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34021 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34081 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34141 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    34201 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    34261 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34321 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    34381 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34441 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34501 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34561 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34621 caccatgtca cgatcgcggg catcaatata aatccagctc gtcgggcgcg cattctcggt
    34681 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34741 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34801 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34861 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34921 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34981 tagagcagaa gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35041 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35101 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35161 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    35221 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    35281 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35341 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35401 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35461 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35521 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35581 actgctttcc ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac
    35641 gatcgccagc agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa
    35701 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35761 cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg
    35821 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35881 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35941 gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36001 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36061 cgcacagact accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat
    36121 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36181 actacttttc agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt
    36241 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat
    36301 gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac
    36361 gccattttct ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36421 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36481 gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg
    36541 ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc
    36601 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    36661 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36721 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36781 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36841 atacacataa gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga
    36901 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    36961 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    37021 cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37081 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37141 gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37201 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac
    37261 gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg
    37321 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg
    37381 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37441 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37501 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37561 agcgtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37621 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37681 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga
    37741 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37801 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37861 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37921 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37981 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38041 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38101 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38161 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38221 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38281 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38341 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38401 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    38461 ctcattttcg tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca
    38521 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38581 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38641 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38701 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38761 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38821 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38881 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    38941 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39001 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39061 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39121 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39181 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    39241 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    39301 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    39361 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39421 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39481 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39541 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39601 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39661 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39721 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39781 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39841 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    39901 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    39961 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    40021 cgggcattgc cgtacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40081 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    40141 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    40201 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40261 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40321 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    40381 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    40441 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    40501 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    40561 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40621 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40681 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40741 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40801 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    40861 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    40921 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    40981 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    41041 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41101 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41161 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    41221 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41281 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta
    41341 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc
    41401 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41461 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    41521 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41581 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    41641 cgcagaccga gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41701 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41761 gcacgccgat taccaatctc attacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41821 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41881 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    41941 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42001 aagatcgcgg cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    42061 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca
    42121 gagagacaat tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42181 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42241 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42301 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42361 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42421 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42481 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42541 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcatcgtta taaacaagcc
    42601 atctatcata taaagcaatg aataatttaa tcgcgattaa tcatcattaa ttgttatatt
    42661 attcactcaa tctatttgtc aagtgaattt gataaattcc taatttaaaa tatccactat
    42721 tgagccagta tttaacaacg ttaagatgtg caatgtgcag atcatgttat cagggtggag
    42781 cggtagcata tatttcttat gcaatcattc attttaataa tgttaattgg attatttatt
    42841 gctgtgaata aaaatatatt atgaatattg gtttaacttt gcgtttttgt tactcgttgt
    42901 gatatgcgta cctgtcctga ataacgccaa gaaacaagag gttgagcttg tgattatttt
    42961 tttgactgaa cagacacccg tttctgtaaa gtaatattta aacaaaattc tcacataacc
    43021 atagcatttt ctgttccttc cctaataacg tctgaagtcg tcccgcttat cacattatcc
    43081 gcagttgtac ggtacggatc attattcatt ttactggagg ttgctatgtc aaccggatta
    43141 cgtttcacac tggaagtgga cggcctgccg ccggacgctt ttgcggtggt ttcctttcat
    43201 ctgaaccagt cactctcttc gctcttttcc ctcgatctct ccctggtcag ccagcagttt
    43261 ctctcccttg aatttgcgca ggtgctggac aaaatggcct acctgacgat atggcagggc
    43321 gatgaagtac agcgccgggt gaaaggcgtg gtgacctggt ttgaactcgg ggagaacgac
    43381 aaaaaccaga tgctgtacag catgaaggtg cacccgccgc tgtggcgtgc cggtctgcgc
    43441 cagaacttcc gtatcttcca gaacgaggac atcaaaagca tcctcggcac gatgttgcag
    43501 gaaaacgggg tgaccgaatg gagtccgctg ttcagcgagc cgcatccttc ccgtgagttt
    43561 tgtgtccagt acggtgagac tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc
    43621 atcttctttt atgaggagca tgcttacaaa agtaccgacc agagcctggt gctgtgcgac
    43681 acagtccgcc atctgcccga atcttttgaa atcccatgga acccgaacac ccgtaccgag
    43741 gtgagcaccc tctgcatcag ccagttccgc tacagcgcac aaatccgccc ttcttccgtg
    43801 gtgaccaaag actacacctt taaacgcccc ggctgggccg gacgttttga acaggaaggc
    43861 cagcaccagg actaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag
    43921 ggtgctcacg ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caatgcagaa
    43981 accgcgcggg gaatgagtcg ctcgccggag atatggccgg gacgacgaat tgtgctgacg
    44041 gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc
    44101 gaacagccac aggcggtgcc aggacggcag ggagcgggga cggcgctgga gaaccatttt
    44161 gcggtgatcc cggcagacag aacatggcga ccacagccgt tgctgaaacc gctggtcgac
    44221 ggcccgcaga gcgctgtcgt gacaggaccg gcaggcgagg aaatcttctg cgacgaacat
    44281 ggtcgcgtgc gggtgaagtt caactgggac cgttataacc cggcagacca agacagttcg
    44341 tgctggatcc gtgtggcaca ggcgtgggca ggcaccggtt ttggccacct ggcgataccg
    44401 cgtgtgggtc aggaagtgat tgtagacttc ctcaacggcg atccggacca gccgatcatt
    44461 atggggcgca cctaccacca ggaaaaccgc ccccccggca gcctgccggg aacaaagacg
    44521 cagatgacca tccgctccaa aacgtacatg ggcagcggat ttaatgagct gaagtttgat
    44581 gatgcgacag ggagagaaca ggtctacatc cacgcgcaga agaacatgga taccgaagtg
    44641 ctcaacgacc gtaccaccac cgtaaaacac gatcaccgcg aaaccgtaaa aaatgaccag
    44701 acggtcacga tccaggaagg taaccgcctt cttacggtgg aaaaaggcca caagatcacc
    44761 ggagtactga aagggtcttt atctgaggat gtctttcagg acagaggcac gattgccggt
    44821 tcggtgcatg ttgacgctgt aaacaatggt ggcgaaggcg acggtataca ggcttatacg
    44881 gcgattaagg aaattttgct ggccgtggag gaaagcaaaa ttgcgctgac gccggatagc
    44941 attcagctac aggtcgggga atcgacggta atcaggctgt cgaaggatgg catcaccatc
    45001 gtgggcggtt ctgttttcat caactgattc actcagatta tcagggagta ttttatgcag
    45061 ttcactttta atgaaggcca cattcaactg ccgtctcaat ggcaggatca gtcaatgcag
    45121 gtcctggtgt ccacagataa cagtggcatc aacctggtca ttacccgcga agccgttcca
    45181 cagggaaccc tgacaccaga gctgtaccag gaaacgctgg cgctgtacca gggcaaactt
    45241 gatgggtaca ccgaacatgc ctgccgggaa atcactctgg cagaggcccc ggcctggctg
    45301 ctggattatt cctggaacgg tccggaagat gaggggaatc aggggcggat tagccagata
    45361 gccgtattcc agcggcgtgg agatacgctg ctgaccttca ctttcagcac ctccttatcc
    45421 ctgaagaaca gtcaaaagac gatgctgctg gaggtgataa agagcttcac cccgttgcca
    45481 ccggagaatg acatccaaaa ggaccagccg cgctgacata aacgagggat aacacatgag
    45541 tgaaggacca ggcgggccac agggagcgac cgcaggcggt acgctggcaa tgcgaatgct
    45601 gtcacagcag gcgatggtcg ccagccagat gaaacgggca gccaacgaca aagccattgc
    45661 acagatgttg gcatcaaaaa agtctggccc ccccgccgcc aggctgggcg atgaaattca
    45721 gcacaagagt tttttggggg cgctggcagg ggccgtactg ggggcgatag tgaccatcgc
    45781 agaaggttgc ctgattatgg ccgcctgtgc caccggccct tatgcgctgg ttctggtacc
    45841 tgcgctgatg tatgccagct ataaggcgag tgattatgtg gaggagaaac agaaccagct
    45901 tgaatcatgg ataaacagct tttgtgacac ggacggcgcc atcaataccg gttctgaaaa
    45961 cgtaaaaatt aacggcgagc tggccgcacg tgcagcggtc acccttcccc ctcctccccc
    46021 acctggagca atacctgaag tcccacaggg ggaaccctca tggggtgata ttgccactga
    46081 cctgcttgaa tcggcagcgg aaaaagcagt accactggcg aaggcctggg ggaacgctgt
    46141 tatcaccctg acggacagca atgccggttt tatggatcgc gtcagcgccg gcgcatcgct
    46201 tctgtttccc gccggtccgg tattaatgga gtttgccacc atggtgggcg ggcgtggcga
    46261 aatcaaaaaa gatgtggatt tcccggaagc cggtgaggac acggcgctct gcgacaagga
    46321 gaacaaacca ccgaggatag cccagggcag cagcaacgtc tttatcaaca atcagcctgc
    46381 cgcgcgcaag ggcgacaaac tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt
    46441 ctttattggc ggtgagcagg tcacatatct ggatatccag ccggagttcc cgccgtggca
    46501 gagaatgatc ctggggggaa taacgatagc cagctatctt ctgccgccag cgggactact
    46561 gggaaaactg aagaatctgg cgagactcgg caaactggga aactgctggg gaaaagcggg
    46621 aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc gttaaaaagt
    46681 attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac cggcgcatac
    46741 tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt cacccgtttc
    46801 cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga ctcctggagc
    46861 gaatacgcct gggtgcgtga acagggaaac cgggtggata tcatcagcca gggagccacg
    46921 ctgagatttg ccttcgacgg tgacagtgat acggcggtta acccgtatca cgcccagtac
    46981 attctgcgcc gccgcgatga ttatctggag ctgttcgaca gggatgcact gagcagccgc
    47041 ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga cgataccagc
    47101 gatgaccgcc tggcacacag ccccaatgac cggatgtaca tgctgggcgg gatgagcgac
    47161 accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac gggtgtcagt
    47221 cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct gaaagccatt
    47281 caccgcacgg ataacgacat acagacgctg gcgacctacg aacaggatgc gcgggggcgg
    47341 ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga cgctgcggac
    47401 cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta cgatgaacag
    47461 ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct ggactatggt
    47521 gacggctgca ccaccgtgac ggacggcaag ggcattcacc gttattacta tgatcctgac
    47581 ggcaatattc tgcgggaaga agcgccggac ggcagcacca ccatgtatga atgggatgaa
    47641 ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaaatt tgaatacaac
    47701 gccgcacacg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg gcagtaccgc
    47761 tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac gtggacacag
    47821 cagtgtgatg aacgcggcct gccggtaagt ctggtgtcgc cacagggcga agagacccgg
    47881 ctggcataca ccgctcaggg gctgctgtcg ggaatattcc gccaggatga acggcgtctg
    47941 ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat gggccgtgaa
    48001 caccacaccg aatacagcgg tcacgacctg ccagtgaaga tgcgcggccc cggcggtcag
    48061 tcagtgcggt tacagtggca gcagcaccat aaactgagcg gcattgaacg tgctggaacc
    48121 ggcgcggaag gatttcgcta tgaccgccac ggcaacctgc tggcctggac ggacggcaac
    48181 ggtgttgtct ggacgatgga atacggtccg ttcgattttc cggtggcgcg aacggacggt
    48241 gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcaactgac agaagtcatc
    48301 aacccgcagg gtgagtcata tctttatgtt ctggataact gcggccgggt gacggaagag
    48361 cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ccgatggcct ctgtaccgcc
    48421 aaggttaacg gcctggagga aaccatcctc tacagccggg acgccgcagg ccgcctggca
    48481 gaaatcatct cgccggaagg caaaacgcag tatgcctatg acaaatccgg caggctgacg
    48541 ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg cgggcgggtg
    48601 aatgtcacca ctcagggccg acgggccatt gaatacaact accccgatga acacaccgtc
    48661 atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccggtga atccctgctg
    48721 aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct gccgggggat
    48781 gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg
    48841 ggttttgcct gtgaacaggg ctggaatgca gccggtcagc ctgtcagtca gcgcgccgga
    48901 ttttttccgg cagaagccac atgggacggg ctggttccct cactggtacg ggagtaccgt
    48961 tacgacagcg cgggtaacgt gtcaggcgtc accagccggg aagattacgg acgggaaaca
    49021 cggcgggagt atcggctgga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc
    49081 gggctgggct atggcgaagg cgatgagtcc tatggctatg acagctgcgg ctacctgaag
    49141 gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc cggaggccac
    49201 cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc
    49261 cgcaccagac accgtgacgg ctaccgaccg gaaacagagc ggttccggtg ggacagccgg
    49321 gaccagctga ccgggtattg cagcgcacag ggtgagctgt gggaataccg ccacgacgcc
    49381 agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtatctgtgg
    49441 gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtaagg
    49501 cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt acggcaggcg
    49561 catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag cgacctgacg
    49621 ggccgcccgc tgatgctctt taacagtgaa ggaaagacgg tctggcgacc ggggcagacc
    49681 agcctgtggg ggctggcact cagcctgccc gcagacaccg gctacccgga cccgcgcggg
    49741 gaactggacc cggaagccga ccccggcctg ctgtatgcag gacagtggca ggatgcggaa
    49801 tccgggctgt gctataatcg gttccggtat tacgagccgg aaaccggaat gtacctggtg
    49861 agtgatccgc tggggttgca gggcggggag cagacttacc ggtatgtgcc gaatccttgt
    49921 gggtgggttg atccgctggg attggctgca agttctaaaa tcagcagttt gatggactat
    49981 attggcgatg gtcgtcgtgt tagtgggcat acgggtttcc tggatggggt tcgtttatca
    50041 cgtagtcaaa taaa
//
LOCUS       DAJTVE010000011        49820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000011 DAJTVE010000000
VERSION     DAJTVE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..455
                     /locus_tag="OO439_000634"
     CDS             <1..455
                     /locus_tag="OO439_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6315089.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO439_000635"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO439_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6315090.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO439_000636"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO439_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6315091.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OO439_000637"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OO439_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6315092.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO439_000638"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO439_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6315093.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO439_000639"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO439_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6315094.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO439_000640"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO439_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6315095.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OO439_000641"
     CDS             complement(6424..6918)
                     /locus_tag="OO439_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6315096.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO439_000642"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO439_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6315097.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO439_000643"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO439_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6315098.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO439_000644"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO439_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6315099.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO439_000645"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO439_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6315100.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OO439_000646"
     CDS             complement(12082..12369)
                     /locus_tag="OO439_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6315101.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OO439_000647"
     CDS             complement(12488..13375)
                     /locus_tag="OO439_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6315102.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OO439_000648"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OO439_000648"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6315103.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OO439_000649"
     CDS             complement(14612..15106)
                     /locus_tag="OO439_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6315104.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OO439_000650"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OO439_000650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6315105.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO439_000651"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO439_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6315106.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO439_000652"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO439_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6315107.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OO439_000653"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OO439_000653"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6315108.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OO439_000654"
     CDS             19086..19745
                     /locus_tag="OO439_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6315109.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO439_000655"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO439_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6315110.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OO439_000656"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OO439_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6315111.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OO439_000657"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OO439_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6315112.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="OO439_000658"
     CDS             23173..24099
                     /locus_tag="OO439_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6315113.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="OO439_000659"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="OO439_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6315114.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="OO439_000660"
     CDS             complement(24450..26669)
                     /locus_tag="OO439_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6315115.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO439_000661"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO439_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6315116.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO439_000662"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO439_000662"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6315117.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO439_000663"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO439_000663"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6315118.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="OO439_000664"
     CDS             complement(32041..32523)
                     /locus_tag="OO439_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6315119.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO439_000665"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO439_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6315120.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="OO439_000666"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="OO439_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6315121.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="OO439_000667"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="OO439_000667"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6315122.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="OO439_000668"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="OO439_000668"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6315123.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="OO439_000669"
     CDS             35847..36161
                     /locus_tag="OO439_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6315124.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO439_000670"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO439_000670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6315125.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO439_000671"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO439_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6315126.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO439_000672"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO439_000672"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6315127.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="OO439_000673"
     CDS             complement(39563..39985)
                     /locus_tag="OO439_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6315128.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO439_000674"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO439_000674"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6315129.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="OO439_000675"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="OO439_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6315130.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="OO439_000676"
     CDS             42449..43120
                     /locus_tag="OO439_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6315131.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="OO439_000677"
     CDS             43126..44286
                     /locus_tag="OO439_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6315132.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO439_000678"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO439_000678"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6315133.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="OO439_000679"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="OO439_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6315134.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="OO439_000680"
     CDS             complement(46086..46256)
                     /locus_tag="OO439_000680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315135.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO439_000681"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO439_000681"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6315136.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="OO439_000682"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="OO439_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6315137.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="OO439_000683"
     CDS             48117..48668
                     /locus_tag="OO439_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6315138.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..49735
                     /locus_tag="OO439_000684"
                     /pseudo
     CDS             48728..49735
                     /locus_tag="OO439_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12492 a        13204 c        12356 g        11768 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc actaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt
    49741 ctatatatca atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt
    49801 gtcacgttta accagtatct
//
LOCUS       DAJTVE010000012        46684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000012 DAJTVE010000000
VERSION     DAJTVE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO439_000685"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO439_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6315139.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="OO439_000686"
     CDS             complement(280..873)
                     /locus_tag="OO439_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6315140.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="OO439_000687"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="OO439_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6315141.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="OO439_000688"
     CDS             1788..2183
                     /locus_tag="OO439_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6315142.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="OO439_000689"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="OO439_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6315143.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="OO439_000690"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="OO439_000690"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6315144.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="OO439_000691"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="OO439_000691"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6315145.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="OO439_000692"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="OO439_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6315146.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO439_000693"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO439_000693"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6315147.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO439_000694"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO439_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6315148.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="OO439_000695"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="OO439_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6315149.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="OO439_000696"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="OO439_000696"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6315150.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="OO439_000697"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="OO439_000697"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6315151.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="OO439_000698"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="OO439_000698"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6315152.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="OO439_000699"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="OO439_000699"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6315153.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO439_000700"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO439_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6315154.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO439_000701"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO439_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6315155.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="OO439_000702"
     CDS             17158..17709
                     /locus_tag="OO439_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6315156.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO439_000703"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO439_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6315157.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="OO439_000704"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="OO439_000704"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6315158.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO439_000705"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO439_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6315159.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="OO439_000706"
     CDS             complement(21354..22376)
                     /locus_tag="OO439_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6315160.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="OO439_000707"
     CDS             complement(22390..23892)
                     /locus_tag="OO439_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6315161.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO439_000708"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO439_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6315162.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="OO439_000709"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="OO439_000709"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6315163.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="OO439_000710"
     CDS             complement(26207..26548)
                     /locus_tag="OO439_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6315164.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO439_000711"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO439_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6315165.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO439_000712"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO439_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6315166.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="OO439_000713"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="OO439_000713"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6315167.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="OO439_000714"
     CDS             33227..34570
                     /locus_tag="OO439_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6315168.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="OO439_000715"
     CDS             complement(34650..34856)
                     /locus_tag="OO439_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6315169.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="OO439_000716"
     CDS             35181..35735
                     /locus_tag="OO439_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6315170.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="OO439_000717"
     CDS             complement(35825..36736)
                     /locus_tag="OO439_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315171.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO439_000718"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO439_000718"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6315172.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="OO439_000719"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="OO439_000719"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6315173.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="OO439_000720"
     CDS             complement(39939..40319)
                     /locus_tag="OO439_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6315174.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="OO439_000721"
                     /pseudo
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="OO439_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
     gene            41376..42341
                     /locus_tag="OO439_000722"
     CDS             41376..42341
                     /locus_tag="OO439_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6315175.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="OO439_000723"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="OO439_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6315176.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO439_000724"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO439_000724"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6315177.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO439_000725"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO439_000725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6315178.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="OO439_000726"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="OO439_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6315179.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46684)
                     /locus_tag="OO439_000727"
     CDS             complement(46338..>46684)
                     /locus_tag="OO439_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6315180.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11456 a        12415 c        11631 g        11182 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgcag
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctagacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggt
//
LOCUS       DAJTVE010000013        45763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000013 DAJTVE010000000
VERSION     DAJTVE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..556)
                     /locus_tag="OO439_000728"
     CDS             complement(<1..556)
                     /locus_tag="OO439_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6315181.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLN"
     gene            complement(570..1229)
                     /gene="pgmB"
                     /locus_tag="OO439_000729"
     CDS             complement(570..1229)
                     /gene="pgmB"
                     /locus_tag="OO439_000729"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6315182.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1226..3493)
                     /gene="ycjT"
                     /locus_tag="OO439_000730"
     CDS             complement(1226..3493)
                     /gene="ycjT"
                     /locus_tag="OO439_000730"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6315183.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3490..4545)
                     /locus_tag="OO439_000731"
     CDS             complement(3490..4545)
                     /locus_tag="OO439_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6315184.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4555..5343)
                     /locus_tag="OO439_000732"
     CDS             complement(4555..5343)
                     /locus_tag="OO439_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6315185.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5361..6413)
                     /locus_tag="OO439_000733"
     CDS             complement(5361..6413)
                     /locus_tag="OO439_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6315186.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6444..7047)
                     /locus_tag="OO439_000734"
                     /pseudo
     CDS             complement(6444..7047)
                     /locus_tag="OO439_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7034..7915)
                     /locus_tag="OO439_000735"
     CDS             complement(7034..7915)
                     /locus_tag="OO439_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6315187.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7936..9246)
                     /locus_tag="OO439_000736"
     CDS             complement(7936..9246)
                     /locus_tag="OO439_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6315188.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9281..10791)
                     /locus_tag="OO439_000737"
                     /pseudo
     CDS             complement(9281..10791)
                     /locus_tag="OO439_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11003..11317)
                     /gene="pspE"
                     /locus_tag="OO439_000738"
     CDS             complement(11003..11317)
                     /gene="pspE"
                     /locus_tag="OO439_000738"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6315189.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11392..11613)
                     /gene="pspD"
                     /locus_tag="OO439_000739"
     CDS             complement(11392..11613)
                     /gene="pspD"
                     /locus_tag="OO439_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6315190.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11622..11981)
                     /gene="pspC"
                     /locus_tag="OO439_000740"
     CDS             complement(11622..11981)
                     /gene="pspC"
                     /locus_tag="OO439_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6315191.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11981..12205)
                     /gene="pspB"
                     /locus_tag="OO439_000741"
     CDS             complement(11981..12205)
                     /gene="pspB"
                     /locus_tag="OO439_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6315192.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12259..12927)
                     /gene="pspA"
                     /locus_tag="OO439_000742"
     CDS             complement(12259..12927)
                     /gene="pspA"
                     /locus_tag="OO439_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6315193.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13094..14071
                     /gene="pspF"
                     /locus_tag="OO439_000743"
     CDS             13094..14071
                     /gene="pspF"
                     /locus_tag="OO439_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6315194.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14189..15454)
                     /gene="puuE"
                     /locus_tag="OO439_000744"
                     /pseudo
     CDS             complement(14189..15454)
                     /gene="puuE"
                     /locus_tag="OO439_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15492..16772)
                     /gene="puuB"
                     /locus_tag="OO439_000745"
     CDS             complement(15492..16772)
                     /gene="puuB"
                     /locus_tag="OO439_000745"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6315195.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16774..18264)
                     /gene="puuC"
                     /locus_tag="OO439_000746"
                     /pseudo
     CDS             complement(16774..18264)
                     /gene="puuC"
                     /locus_tag="OO439_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18414..18971)
                     /gene="puuR"
                     /locus_tag="OO439_000747"
     CDS             complement(18414..18971)
                     /gene="puuR"
                     /locus_tag="OO439_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6315196.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18998..19762)
                     /gene="puuD"
                     /locus_tag="OO439_000748"
     CDS             complement(18998..19762)
                     /gene="puuD"
                     /locus_tag="OO439_000748"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6315197.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19974..21335
                     /gene="puuA"
                     /locus_tag="OO439_000749"
     CDS             19974..21335
                     /gene="puuA"
                     /locus_tag="OO439_000749"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6315198.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21484..21648
                     /locus_tag="OO439_000750"
     CDS             21484..21648
                     /locus_tag="OO439_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315199.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21638..23023
                     /gene="puuP"
                     /locus_tag="OO439_000751"
     CDS             21638..23023
                     /gene="puuP"
                     /locus_tag="OO439_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6315200.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23157..23402
                     /locus_tag="OO439_000752"
     CDS             23157..23402
                     /locus_tag="OO439_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6315201.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23715..25358
                     /gene="sapA"
                     /locus_tag="OO439_000753"
     CDS             23715..25358
                     /gene="sapA"
                     /locus_tag="OO439_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6315202.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25355..26320
                     /gene="sapB"
                     /locus_tag="OO439_000754"
     CDS             25355..26320
                     /gene="sapB"
                     /locus_tag="OO439_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6315203.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26307..27197
                     /gene="sapC"
                     /locus_tag="OO439_000755"
     CDS             26307..27197
                     /gene="sapC"
                     /locus_tag="OO439_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6315204.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27197..28189
                     /gene="sapD"
                     /locus_tag="OO439_000756"
     CDS             27197..28189
                     /gene="sapD"
                     /locus_tag="OO439_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6315205.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28191..28997
                     /gene="sapF"
                     /locus_tag="OO439_000757"
     CDS             28191..28997
                     /gene="sapF"
                     /locus_tag="OO439_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6315206.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29065..29418
                     /locus_tag="OO439_000758"
     CDS             29065..29418
                     /locus_tag="OO439_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6315207.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29786..30574
                     /gene="fabI"
                     /locus_tag="OO439_000759"
     CDS             29786..30574
                     /gene="fabI"
                     /locus_tag="OO439_000759"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6315208.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30719..31845
                     /locus_tag="OO439_000760"
                     /pseudo
     CDS             30719..31845
                     /locus_tag="OO439_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            31913..33847
                     /gene="rnb"
                     /locus_tag="OO439_000761"
     CDS             31913..33847
                     /gene="rnb"
                     /locus_tag="OO439_000761"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6315209.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(33925..34028)
                     /locus_tag="OO439_000762"
                     /pseudo
     CDS             complement(33925..34028)
                     /locus_tag="OO439_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34082..36072
                     /gene="pdeR"
                     /locus_tag="OO439_000763"
                     /pseudo
     CDS             34082..36072
                     /gene="pdeR"
                     /locus_tag="OO439_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36219..36392
                     /locus_tag="OO439_000764"
     CDS             36219..36392
                     /locus_tag="OO439_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315210.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36482..37231
                     /locus_tag="OO439_000765"
     CDS             36482..37231
                     /locus_tag="OO439_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6315211.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37500..37718
                     /gene="osmB"
                     /locus_tag="OO439_000766"
     CDS             37500..37718
                     /gene="osmB"
                     /locus_tag="OO439_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6315212.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37844..38170)
                     /gene="yciH"
                     /locus_tag="OO439_000767"
     CDS             complement(37844..38170)
                     /gene="yciH"
                     /locus_tag="OO439_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6315213.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38170..38907)
                     /gene="pyrF"
                     /locus_tag="OO439_000768"
     CDS             complement(38170..38907)
                     /gene="pyrF"
                     /locus_tag="OO439_000768"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6315214.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39100..40269)
                     /gene="lapB"
                     /locus_tag="OO439_000769"
     CDS             complement(39100..40269)
                     /gene="lapB"
                     /locus_tag="OO439_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6315215.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40276..40584)
                     /gene="lapA"
                     /locus_tag="OO439_000770"
     CDS             complement(40276..40584)
                     /gene="lapA"
                     /locus_tag="OO439_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6315216.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40733..41497)
                     /gene="pgpB"
                     /locus_tag="OO439_000771"
     CDS             complement(40733..41497)
                     /gene="pgpB"
                     /locus_tag="OO439_000771"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6315217.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41667..42257
                     /gene="ribA"
                     /locus_tag="OO439_000772"
     CDS             41667..42257
                     /gene="ribA"
                     /locus_tag="OO439_000772"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6315218.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42321..44996)
                     /gene="acnA"
                     /locus_tag="OO439_000773"
     CDS             complement(42321..44996)
                     /gene="acnA"
                     /locus_tag="OO439_000773"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6315219.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45160..45255)
                     /locus_tag="OO439_000774"
     CDS             complement(45160..45255)
                     /locus_tag="OO439_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315220.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45369..45536)
                     /locus_tag="OO439_000775"
     CDS             complement(45369..45536)
                     /locus_tag="OO439_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315221.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45539..45703)
                     /locus_tag="OO439_000776"
     CDS             complement(45539..45703)
                     /locus_tag="OO439_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6315222.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10806 a        12097 c        11527 g        11333 t
ORIGIN      
        1 tattcagctt ctggtgcagc ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat
       61 caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc
      121 cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact
      181 cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga
      241 ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg
      301 cgtagttctg gaacaccatc gctatattgc gtgctttggc tggaacgtca ttcattcgtt
      361 tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg
      421 tggtcgactt accgcagcct gacggaccga caaacacgat gaactcttta tcggcaattt
      481 ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta
      541 acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc
      601 gaccaggtca gggagtccgt tgaaggcaat agtaattgcg ccccggttat gcccgcgccg
      661 atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca
      721 atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag aaagatttcc
      781 ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc
      841 agctccagtg catctaaaat cgtcggcgca ttgagggaga cagaagccag ccctaccgag
      901 atctgttgtg cgcggagatc tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc
      961 gtcaactcgc gcaatgagtg gacatagagc agatttttgc gatacgccag ttgcgccctc
     1021 tcctgcgagt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca
     1081 tcgcggctga tcccttttag ggattcgtta aactgcgcat caatgctgat gccaatttca
     1141 gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc
     1201 agatcgaaaa ttacctcttg cagtttcatt catcctcctg atgtgtggca gcggtcccat
     1261 tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct
     1321 gaccgttcaa acgcagtgaa acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa
     1381 gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct
     1441 caggtaacgc cggattgaga tgcaattcac cgtcacgcac gctccccccg gcaaaaccct
     1501 gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca tcacaactat
     1561 gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca
     1621 gcaggccaca gcgtgcggca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa
     1681 tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgctgag aactgctccg
     1741 gcagcatgta attgagcatc accacatcag cttgtttgag gatttgcatc tcgttcactt
     1801 ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg tatttcgcaa
     1861 gattaatcgc cggcttagcc ataaacgaat catcctgcgg caaaacgccg tcgggctgaa
     1921 tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc
     1981 tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac gccatatagc
     2041 tggtgaaggc gttattattg acatgttcgg tatattcgtc aggcccaata acatcatgaa
     2101 tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa
     2161 gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa
     2221 taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt
     2281 gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct tcgccgctgc
     2341 gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg
     2401 cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa
     2461 acagatgaaa cggtaacaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt
     2521 cgccggttaa gccttttgcc gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc
     2581 gcagatgata aagcgcataa tctaacgctt gctgatcgtg cacttcgccg ccatttaccg
     2641 tgatacgacg tttctgccac cattgacgcc agttttctgt tgatactgca agaagttggt
     2701 cgtaactctg ctgcgcgcac atttcaagct ggcgaagcga cgcgctgccc cactcgtcta
     2761 aaacggcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct
     2821 cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc
     2881 attgctgcac atcaccgctc accttacagc aacagctgat ggccacatca ctgcgtccat
     2941 cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt
     3001 cgagatgctg gcgaccgtgg ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg
     3061 acgcgtcggc gtccagtggc gtaatagtga tttccagcac aatgagcggc agttggtctg
     3121 ccgaaacaaa gcgacggctg gcgatggtgt aacctgtgcc gttgctggta cgccagacaa
     3181 cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata
     3241 acgagaaaac ctcaccattt atggcaatct ccatccccac gacatcaggc aggttcacca
     3301 gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg
     3361 tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg
     3421 ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta
     3481 ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgataca acgccgccac
     3541 cagttgctgg atgatgtacc cctgcttggc gtcggctatc atcacgggct tgccctgtac
     3601 gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg
     3661 catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc
     3721 accagcttta tcaccacaaa agctgacgtt cataatcgac tgttcgcgga tgttgagtgc
     3781 aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga
     3841 atcttcgacg ctgtaagttg ccggatccca ctcaccaaat tgaccacagc tcttttgcgt
     3901 gccgatcttt tgaaagctat gcgcattcac gcttttcacc gccggaaagc ccagcacata
     3961 catcgcagca tccagcatat gaatgccgat gtcgatcagc gggccaccac cctgcagttc
     4021 tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt
     4081 aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag
     4141 cgcaaaacga tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca
     4201 catttcccgc gcctgttctg gcgtcatggc gagcggtttt tcgcacatca catggcagcc
     4261 cgcttccagt gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc agacgctaac
     4321 cacatcaggt ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc
     4381 gtatttttct gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc
     4441 cagatcgttg cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcctgcgcc
     4501 tattatcgcg acccgcagcg gagagcttgt cattgcactt ttcacaaaga cctcttagca
     4561 ggtacgcaac caggccaacg aatcacggta cgcctgggca ggatcttccg cccggatacg
     4621 cccttcgtac actacatagc cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg
     4681 gaaatccagg gtgccgctgc ccggctggta gcgatggtta tccgcaatat gcacatgacc
     4741 gagcaggtcg cggttgtcat gcagcgcctg cgccaggtta tcttcttcga tattcatgtg
     4801 atagaaatcg ccgataatct gtacatgttt aagatcgttt tcgacgatgt aacggcgggc
     4861 atcggcgagg gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac
     4921 tgttccggta cgcgcggcga cctgttccag tacgcgcagg gaatcactca ccattttgcg
     4981 gtcgccgtcc aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca
     5041 cgctgccgga acgacgatac ctttatcccc cacttctgcc agcgcttcga gaatgcgttc
     5101 gatctgcttt aagccattaa gacgacgctc ttcgataaag tcgccaatcc aaccgtcata
     5161 gccaccgcag gcggtggtca ccggtaaacc ggtttctttg attgctgctt tgacttcttc
     5221 gagattgttg accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat
     5281 ataacgaaat ttctccagaa tgttttccgg aaagaatgcc tgattctgtg tgcctatttt
     5341 cattttttca ttcctttgaa ttaaaacgta acgcccattt tgatgctctg ttccggatgc
     5401 tgatcgacat actgcatgta gctttccggg ctattggcaa aggtcactac cgggtcgatt
     5461 aaatcttcgc aattgagata accgttcatc agcagttccc agcaggtttc ttcaatacgc
     5521 ttacggctcc agcgcggata atccgggttc ggttcgctgc acgcgcggga gaaaacaatt
     5581 ttggcgttat tgaaatgcgc ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca
     5641 aacgcaacat aggagatggt gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg
     5701 tcggcatagc cgctggtttc gataatcaca tcggcaccct gcttgccggt cagcgttttg
     5761 atctctttgc ctacgtcagt accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg
     5821 gcaatatcac agcgatgagc gataggatcg acgccaatca cgaccgacgc gccagcacgt
     5881 ttagccagtt gaatggcaat ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca
     5941 cccacgcgca cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa
     6001 acggcgtttt tccacgagct gccttccggc attttgcgca gcttgtagtt attcaccgcg
     6061 ttaatgatga ccgtctcgga gagcgggccg tagccgcata cgctgtcgcc caccgcgtag
     6121 tcggtaacgt cgctgccgca ctcgataatg tcgccaacca ccatgtttcc aagctggaat
     6181 ttgccaaact caatgccgcg cggcgcatct gccggacgcg gagtgaacat ctgccattcg
     6241 ccgttaaagt cttcatcgat aaacgggctg gcggcgcgga agtcgaccac ttccgttccg
     6301 tgtttcggtg cgccgaaacg cacgcggacc ttcacttcat tagctaaaat cgcccgatct
     6361 tcatactcaa ccagcgcagc aacacgcggt gctgtggcta ctaacttttt catttaaaac
     6421 tccttgtaaa gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta
     6481 ataaaacgtt ccgaaagcgc atacataatc accaccggca atgcggtcac cagtgaggcc
     6541 gccatcatcc gtccccagat gtaatctggc gtactgaaca gcgcgttcag gcccaccggt
     6601 aaggtgaaat tgccggcgct ggagaggaaa atcgacgcaa acagatagtc gttccacgcc
     6661 accataaagc agtagacgaa cacggatatc agcccggaca tcgccaacgg cacggtgatg
     6721 cggaagataa tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatcg
     6781 gggatggtgt cgaagtagct tttcagcata aacacggcgg tgggcagagt ttgcgtcacc
     6841 atggtgatga tcagcgccat ctcagtcgac gtaatgctcc agcgtccact gctgcggcag
     6901 cagcgtagga tgcagtgaga tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac
     6961 aaaaaatgga aacagcgtga tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg
     7021 actgagtgtg cgtttatttg ttgccattga ggttcacccg tttcctggtc agcagaatga
     7081 cagcgaaaat aatgatgaag agcaccaccg aaatcgccgc cgctttccct aagtcattaa
     7141 aggcaaaggc ggttttgtag agatagacgc cgagaatatc gaccttggtg gtcagcagat
     7201 aaacatcagc aaacatgtag aacatccaga tagtgcgcag ggtcaccacc gtcgccagga
     7261 ccggcataat tgcgggcagc gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca
     7321 tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga aacgagataa
     7381 aggcatacgg gaagtagcgc cagatggcga acagcaccac cagcacaaag ctactgcccg
     7441 gattatcgaa ccacagcggt gcctgctcat agagatgcag aagatcgacg ccgaggtagt
     7501 tcacaatgcc gtagccgttg ttgaacatgt atttccaggc aaacaccagc gaaatggacg
     7561 gcgttacgta ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt
     7621 tgaaaaacat cgccactgcc agcccgagca cggtgctgcc cgccaccacc agcgcggtat
     7681 accagaccgt catccacagc gagtgccaga agccgggatc ggagaggata cgcacatagt
     7741 tactcacccc aacaaaggtt gactcgatgt tgggattgag cggcagacgt aaaaaactga
     7801 tttcgatatt cgacaccatt ggccaggcca ccagaccacc cagcagtaat aagctgggcg
     7861 cgagaagcag cagcgcaaag ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg
     7921 attcctgtcc tgggtttagt gctgttcgat cagttcatcc agctttttct ggctcgcttg
     7981 cagcgtgcca gggagatcgg ctttacccac ggtgacgtta tgcaccatgg agctcaccac
     8041 gccagaaccc gtcacatcac ccatgcgggt aaagttttta tcccctactg cgccaaagac
     8101 ctgaatattt ggcagttcac cgattagctg attcggtagt tcacccagcg ccttaataac
     8161 gtcgttgtct ttccaggtgg cggtagtgac aaccgcttta ttcaccggca gcgcagcacc
     8221 tggcgacatc atcacccagt cggcaatgtt gtctgcctgc tccataaagg tgacaaattt
     8281 ttctgctgct tccgtctctt cggccttttg cccggcggta atggtcagcg aggtcaacat
     8341 gccgtagacc gcagagtttt tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc
     8401 ttctttaatt acagccggaa ggatataggt ggagtaaatc gccatcggcg cggtgccgtt
     8461 cataaaggcg tctttcactt ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg
     8521 catagtgttg gcagcaaggt cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc
     8581 aagggtgatt ttgccttcgg cgttaaagac attagcctgg ttggataacg caaactggga
     8641 gaaggattgt tccgtcaaca cgctttctgc tgtaggcagc gcaatgccgt attttttatt
     8701 cgccgggtca ttcagtttct gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc
     8761 cagccccgct tttgccagca catctttgcg ataccagatg ccgccaatcc aggcgctgac
     8821 gggaacaccg gtccaggcac tcccatcttc ggtgcgcaca atacgcagta cgccatcgta
     8881 aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc gtcttgcgat cgataagctg
     8941 ctctttatcc ataactttgg cgtagtcatg gctggtttcg atcacttccg gcagcgagcc
     9001 gctacgtgaa agggtgatga ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt
     9061 aacggtgata cctgggtttt ctttttcaaa acgggcaatc aatttgctga taactgcctg
     9121 acgctcctgc tccaccgaag aatgcataaa ttcgatggat acatgggttt tattttcttc
     9181 tttacagcct gaaatcaggg cgcaggaaac cagtgctgat aacagcacga tttttgattt
     9241 aatcatgaga tgttcctttt tatttaatcc acataacctg ttaggggcgc agcgttattt
     9301 cattacccaa tagatcaact tcactaatca gatcccgacc atgcagatta gtaatattta
     9361 catgctgaat atttttactg acattaaaca atccagtcag acaatcacca tcagcgttac
     9421 ttctttgaat acgcattacg gatgaattaa tcgtatcgat ggtaaaatta ttatccggat
     9481 gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga caactcatga tataccgcat
     9541 gccttaatgt agcttcatcg ttcagttctc gggttatctc tttactgtga tattttttac
     9601 ggttaatcgc acggttatat ccgagttttt cgacacctgc gtaatcatta cgcgagccaa
     9661 gaatactttg aatatatatc gctggcacac cggggaaact taacaaaatc gcatgggcga
     9721 gaataaacct ggcgcaacgt tcttcatcgc tactctcacg gcggcttaac gcatccatat
     9781 gtgagaggcg aggaagttaa accaggtggt gttgctggaa ggtagtgtca ggttttgcgc
     9841 ccacgcacaa agcgcctcaa cgttctgttt ttgcaccgca tgcagcacca gtggcggcag
     9901 cgagaactgg tacaccatat gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc
     9961 tttatgcgga acattggtct cggtaatgat cactgtacct ggcgcaacgt tatcaataat
    10021 cgaccgtaac agtttgataa tcagatgtgt tttttccaga tggatgcagc ttgttcccgg
    10081 ctctttccac ataaagccaa cggcatccag gcggacatac tcagcacctt tcgcaaggta
    10141 acagagtaaa acatccacca tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat
    10201 ttgatcgtca ctaaaggtgg tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg
    10261 cgttaataac ggtaacgcac gcgggcgagt gacggcgctg agatcggttt gcggatcaac
    10321 cgcaataaaa aaatcttcaa aacctggctg ctgttgtaaa tagtttttaa accattcact
    10381 ttttgccgac atatggttgc agacaaaatc aaacattaaa tgactgcatt caccgagttg
    10441 ctgaatatcc tgccactccc ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa
    10501 cccatcatca gatgaacatg gataaaaagg taataaatga acatgtgaaa aaatgctttg
    10561 cagccattga tggtaaaact gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa
    10621 ttgatcggca taggtaatta acacgacatc actttcatcc cagtgttttt tacgtcgctg
    10681 tgtaattaat cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat gagttgcggt
    10741 aaatgttcca ccgtagattt cgtccaggta atccgtaatt ttctgtttca tgttgccttt
    10801 ttgtggtagc ggtcccatga aacgagagac tactccgcag aaaataaact gtcaattgag
    10861 cacaagggtt ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg tatttgtgag
    10921 ctgacgcaaa taatctatgg ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa
    10981 gcgccgctca tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc
    11041 caccggcgtt ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcttgccctg
    11101 actggcgtcc ggcattgcaa tacactttca cggtgtcgtt tttatccgga acggcggtgg
    11161 caatgcgctc tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct
    11221 gatactgctc tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa
    11281 acaccagtgc cagagcaagt aagccttttt taaacatgta agcgtccttc tatggatgaa
    11341 aattgccatc cattatacgc ctgagattta acaatttttg cgaaactccc ctcacctttt
    11401 ataacgctgt gccagtttat tagcagcccg acttaacagc ggttccagcg ccacagccag
    11461 caacattttc agcggtcggc gagcaactga ttttatcgcc cagcccgcta cacccgccgg
    11521 gccatagcgc agtgcggtaa gaagtaccag cttgcctgct aatttgaaac caggctttac
    11581 cttttgcccg gcctgttgcc agcgagtatt cataactttc ctcacagttg acggaaacgg
    11641 ctacgtaacg tgaaagtatc ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca
    11701 cttgccgcca gttcacggtc gacttcatcc agcaattcgc tgctggaagg tagctgctca
    11761 ccaaaggcca tgttgtccgg cattggatca agcgcaaatg acaaaatgat gtaagcaacc
    11821 agggtaaaca gcgccagacc gaagaaaatc gacagcacca ccaggatacg caccagtttt
    11881 accggtacat caaaatagtt ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt
    11941 ggaatacgcc ataatttttt attgagatta atgcccgcca ttagcgatcc ctccagttcg
    12001 gatgttctgc atcaagaata gattccagcg cctgaatacg ttcgcgcatc cgttttgctt
    12061 catcagccag ttgcgctaat cgctgctgct cactttgcga caattcatcg cgaccagaac
    12121 gattgctgta atgcagccat aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg
    12181 gaatagccag aaatagcgcg ctcataagta ctccttagac gatgagcggc ggcgcgttcg
    12241 gacgccgcct ggatgttatt attgattgtc ttgcttcatt ttggctttta attgtgccag
    12301 ttgttcgctg attgcatcat cggctttcag ttcggcaaac tgatcgtcca gcgatttttg
    12361 tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga
    12421 ttcgaaacga gccattgctt catccagttt gccactgtcc agctgacgac gcacatcgcg
    12481 cgacgagttt gccgcctgat gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct
    12541 caatttgttt tccagctcgc caatctcttt cttcatgcgt gccagcgtat cgttcaccag
    12601 cgtcacttca tgttccaggg acttaatcag atcggtcagt ttctgttttt caattaacgc
    12661 tgcacgtgcc agatcctctc tctctttcag cagcgccagt tcggcttttt cctgccattc
    12721 aacttcacgc gccgacgctt gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc
    12781 acgcgccgaa gtagaacgta cttcaaccag tgtatcttcc atctcctgga tcatcagacg
    12841 caccagtttc tgtggatctt ccgctttctc taacagagcg ttgatgttgg cattcacgat
    12901 gtcggcaaag cgagaaaaaa tacccataat gttgtcctct tgatttctgc gttcaggatt
    12961 gtcctgctga actgttaata caatttgcgt gccaattttt tatctttttg atttataaag
    13021 atctgattga agaatcaaca gcaacatgcc aggatgaatt agcgaattac actaacaagt
    13081 ggcgaatttc atcatggccg aatacaaaga taatttactt ggtgaggcga acagctttct
    13141 cgaagtgctg gaacaggttt cgcatctcgc accgctggac aaaccggtgc tcatcatcgg
    13201 cgaacgcggc accggtaaag agctgattgc cagccgcctg cattatctct cctcccgttg
    13261 gcaagggccg tttatttccc ttaactgcgc ggcgttaaat gaaaatctgc tggattccga
    13321 actgtttggt cacgaagcgg gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt
    13381 tgaacgtgcc gacggcggta cgctatttct tgatgaactc gctacggcac cgatgatggt
    13441 gcaggagaaa ttattgcgcg tgattgagta cggtgaactg gagcgcgttg gcggcagcca
    13501 gccattgcag gtgaatgtgc ggttggtatg cgcgacgaat gccgatctcc cggcgatggt
    13561 caatgaaggc acttttcgcg ctgacctgct cgaccgactg gcttttgatg ttgtacaact
    13621 gccaccactg cgcgagcgcg aaagcgacat aatgttgatg gcagaacact ttgccatcca
    13681 gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt acggagcgcg ccagagaaac
    13741 attgctgaat tatcgttggc cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc
    13801 agtgtatcgc cacggcacca gcgattatcc gcttgatgac atcattattg atccctttaa
    13861 acggcgtccg cctgaagacg ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc
    13921 gctggattta cgtgagtttc aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca
    13981 aaaggggaaa tataaccaga agcgcgcagc tgaattgctg gggttaacct accatcagtt
    14041 ccgcgcgttg ttgaaaaaac accagattta gcgaagttgg gcaatgcgat tcgccggatg
    14101 cggcgtgaac gctttatccg gcatacataa tcaacgtcgt ttgcaggcct gataagcgca
    14161 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
    14221 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaagcga atcacgttgc
    14281 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
    14341 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg
    14401 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
    14461 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg ttgataatat
    14521 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
    14581 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
    14641 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
    14701 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
    14761 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
    14821 cgccctcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
    14881 atttaaacaa gcgttcgatg gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg
    14941 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
    15001 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
    15061 caatcacgcc agggcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
    15121 ccgcaccggt agtgaagaac gcggttttgg cctgcccgct caccggggca agggcgttga
    15181 ttttctccgc cagggtgacg tagctttcat acggcacaat ctgatacgcg gtgtgggtaa
    15241 actgttgcag ttgctgctcc accgccgcga ccagatcagg gtggcgatgt ccggtattca
    15301 gcaccgcaat gcctgcggcg aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag
    15361 cgttttcagc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
    15421 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
    15481 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt accaggctgc gcccatcgcg
    15541 gtaaacggta ctcgcagcgt gcgcccgccg gggaacgggt aatacggcag attggcgaag
    15601 gcatcgaaac gttcggcgtc gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga
    15661 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcaaggcga
    15721 ccaaactgcg gcatgcgcga cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt
    15781 ttgacgccct tcagttgcgg gaaggttttc agcagtttcg gcacaacaag acgctcaacg
    15841 tcatccgggt cgcgcgcgcc gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg
    15901 gtaagacggt agtaatcaag cagataatta cagtcttcca cacagtagtt tttcgggatc
    15961 agcgaacggg ctaaatcttc cgacagccgt tcagtggtga tcacctgcgt gccgcacggc
    16021 atgctgcgtt tcgccagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
    16081 atcacatact tcgccgtcac ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc
    16141 tggatctgcg tcacggcaga aagctcatac acgcgcccgc cgttcaggcg gatggcatcg
    16201 gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc caacagtgcg
    16261 ccagtgtagc gatcgctggc gacctcgcgg cgaatggcgt tagcgtccag cagctccagc
    16321 tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc gagctgttta
    16381 tcattcatcg ccacaaacag gccgccgggg cggtagtcgc agtcaatctg atagcgtttg
    16441 atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat acgggcggtg
    16501 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
    16561 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
    16621 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
    16681 caaacgtcgc aggtgattga ctcattcagt gtgtcgaatg gtgcatattt attcgcactg
    16741 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
    16801 tttcagttca gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
    16861 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
    16921 accggctttc aggcggcggc tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc
    16981 cgcgccaagg ccgtactggc tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa
    17041 acgcgtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
    17101 aacatccaca aagatggtcg ggccgatggc ggcagccagc tcagcgttac gaccatccag
    17161 cagcagttgc cctttgcttt cgccttcacg aataaagcta tggaccgagt cggcgtgggc
    17221 gcaatcgatt aaggtgccca tggtggttac ggtatcaagt ggatggcccg gctgccagtt
    17281 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagtaacaa
    17341 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
    17401 tgccgctttt tgcagatccg gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc
    17461 ttccagccag acgcgtttca tgttgctatc acccgcgtct ttcagcagtt gtttacccgt
    17521 acgcgtggag ccggtaaagg cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc
    17581 ggcttcatga ccataaccgc tcactacgtt cagcacgcca tccggcagcc ccgcttcttc
    17641 ttttgccagc ccggcgagac gaatcgcact gagtggtgat ttttcagacg gttttagaat
    17701 cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca gcagcgggaa
    17761 gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca tcgccagctc
    17821 atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg cgtaccagcg
    17881 aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg gtttgccggt
    17941 gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat cggcgagttt
    18001 attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc gttcaaatac
    18061 gccgcgtgct gcgctcaccg catggtcgat atcgacgctc ttgccgcggg caattttcgc
    18121 cagcggtgcc taggtgaccg gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt
    18181 atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt tatcctgcca
    18241 gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag atatatgcag
    18301 tttgtgaacg tcagggcgca gagatccccg ggccgcatgc ccgtttattt ctggcgacat
    18361 tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac
    18421 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
    18481 tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca
    18541 ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta
    18601 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
    18661 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag
    18721 tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
    18781 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
    18841 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
    18901 cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc
    18961 tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga
    19021 tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc
    19081 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa
    19141 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
    19201 tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt aacaacgcag
    19261 acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
    19321 gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca
    19381 acgaaccacc ggttgccacc accagttctt gtaaaccccg gcagatggcg aaaatgggga
    19441 tgcgcctttc gagtgcggca ttaattatcg ccatgctcag aagatcacgc ccgggatcgg
    19501 cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactaccag
    19561 gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt tccgccagcg
    19621 catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt
    19681 gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg
    19741 gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta tcgccattac
    19801 tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt
    19861 tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg aatggtggtc
    19921 attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa
    19981 ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct
    20041 ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt
    20101 tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga
    20161 agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt ctgggcaacg
    20221 tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg
    20281 ttctcggttc cttaactcct tctgccgccg atccagagtt tatcggtcag atgctcctga
    20341 ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc
    20401 tgtggcagca gctgcgccag cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg
    20461 gcaccgatga ccgcaataca caaagccagg tttactccgt tgataacctc aaccacttcg
    20521 ccgacgtgct caatgatatt gatgaactgg cgcagttgca gctgattccg gcagatggcg
    20581 cggtcgctga ggcctcgccg ggtcagtttg aaatcaacct ttaccatact gataacgtgc
    20641 tggaagcctg cgatgatgcg ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc
    20701 ataagatgca cgccactttt atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc
    20761 atatccatat cagtatgcaa aataatcgtg gcgagaatgt gctttctgac acagaaggcg
    20821 aagattcgcc gctgctgaaa aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga
    20881 tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt ccagccggga atgtatgtgc
    20941 cgacgcaggc gtcgtggggc cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg
    21001 accgtcataa tcaccgcgtg gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg
    21061 tgatggcagc gatttttgcc ggtattttgc atggccttga taacgagctg ccgttgcagg
    21121 aagaagtcga aggcaacggg ctggaacagg aaggcttacc cttcccgatt cgccagagcg
    21181 atgccctggg tgagtttatc gagaatgatc acctgcgccg ctatttaggc aaacggtttt
    21241 gccatgtgta tcacgcctgt aaaaatgatg agctgttgca gtttgagcgc ctcatcacag
    21301 aaacagaaat tgagtggatg ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg
    21361 gcctgagcgg agccaggcct gacatttgct ggtaatccag acatttcccg gatgtcgcgt
    21421 tatggagcgc aggcggcaac gggcttccgt taacgattaa gactgtgacg aggaaatgag
    21481 atgatgccga tgattaaatc cccgcatggc gagggaggct gcgtatgcgc ccctcctgct
    21541 actgactgga ctccgccccc tttgcttccc ttgctaaaca ggtttgattt caggtcgaca
    21601 cgaccgcaaa ccttattacg caggggaggc agcaattatg gctattaatt caccactgaa
    21661 tattgctgcg caacccggca aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt
    21721 aatgatgggt ctggcctatc tcacgccgat gaccgtattt gatacttttg gtattgtctc
    21781 cggcattagc gacggtcacg ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt
    21841 taccgctatc agctacggca aactggttcg ccagtttccg gaggccggtt cggcctatac
    21901 ctacgcgcaa aagtcgatta acccgcacgt cggatttatg gtcggctggt catcactgct
    21961 ggattatctc tttttgccaa tgatcaacgt tttgttggcg aaaatctatc tctccgccct
    22021 cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc
    22081 gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat
    22141 ctccatcatg gtggtgttta tcttcctggt ggttcaggga ctgcataaag gagaaggcgt
    22201 tggcaccgtc tggtcacttc agccgtttat cagcgagaac gcgcacctga tcccgattat
    22261 taccggtgcg acgattgtct gtttctcgtt cctcggtttc gatgcggtga ccacgctttc
    22321 ggaagagacg ccggacgccg cacgcgtgat cccgaaagcc atcttcctga cggcggtata
    22381 cggcggcgtt atctttatcg cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag
    22441 ccgctttaaa gacccggacg ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct
    22501 ctttcagtcg attttcctct gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc
    22561 acatgccagc gtgtcgcgtc tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg
    22621 cgtgtttggc tatgtgcacc caaaatggcg gactccggca ctcaacgtca ttatggtcgg
    22681 gattgtcgcg ctgtcggcgc tgttctttga tttagtcacc gcgacagcac tgattaactt
    22741 cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg
    22801 taaaggaatg aataaaagct ggaaggatca cttccactat ttgctgatgc cgctggttgg
    22861 cgcgctgacg gtgggtgtgc tgtgggttaa cctagagtca acgtcactga cactcggtct
    22921 ggtatgggct tcgttaggcg gcgcatattt gtggtatctg atccgccgct atcgcaaagt
    22981 gccgctgtac gaaggtgaca gaacgccggt gagcgaaacg taatagccgg atgcgcggcg
    23041 cattatcctg cctgagggta acgcgccgcg ccattaacta taaaaagatg tataacccgg
    23101 atcacgagtg ctatcatccg cccttatgct tagcgcatta accccagggg caaataatga
    23161 accacgatat cccgctaaaa tattttgata ttgccgatga gtacgcgacc gaatgcgcag
    23221 agcctgtcgc agaggctgaa cgcacaccgc tggcccacta cttccagtta ctgctcaccc
    23281 gtttgatgaa caatgaagag atcagcgaag aagcccaaca tgaaatggct gctgaagcgg
    23341 ggattaatcc cgtacgcatt gatgagatcg cagagttttt gaatcaatgg ggtaatgagt
    23401 aattatttaa ttgcagacga tcggtcagta tcgttgtccg gataaagcgt tcacgccgca
    23461 tccggctaac aatgacacgc actgcagcgt gctttaatgg gtttctcacg atcgtaccgc
    23521 tctgctcctc gctaagttgt tctgtgagtt atagttatag agaaatcacg ggtacgaatg
    23581 ttgaacagat ggcgacgcca cctgttcaac gaccaaccac acggccctca ttacgacccc
    23641 tgaacacaaa cggtaatggc aaatgtcatt aaatattagt aagatagcct ctttacataa
    23701 aatcccttaa tattatgcgc caggtattat cgtctctttt ggtgattgct ggacttgtga
    23761 gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc tgatatccgc gacagcggtt
    23821 ttgtctattg cgtcagcggg caagtcaaca cctttaaccc atccaaagcg agcagtgggt
    23881 taattgtcga tacccttgcc gcccagtttt atgatcgact gctggatgtc gatccctata
    23941 cctatcgcct gatgccggaa cttgccgaaa gctgggaagt actcgacaac ggcgcgacct
    24001 atcgcttcca cctgcgtcgc gatgttccgt ttcaaaaaac cgactggttt actcccactc
    24061 gtaaaatgaa tgccgacgat gtggtgttta ccttccagcg aatttttgac cgcaacaacc
    24121 cgtggcataa cgtcaacggc agcaacttcc cctacttcga cagcctgcaa tttgccgata
    24181 acgtgaaaag cgtccgcaaa ctggataatc ataccgttga gttccgactc gcccagccgg
    24241 atgcttcttt tttgtggcac ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg
    24301 cccggaagtt agagaaagaa gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg
    24361 gtccgtatca gttgtcggaa taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg
    24421 acttctggcg cggtaaaccg ttaatgccgc aggtagtggt ggatttaggc tccggcggca
    24481 ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca
    24541 gccagctatc cattttgcgt gacgacccgc gcttgcgttt aacgctgcgt cctgggatga
    24601 acgtcgccta tctggcattt aacaccgcca aaccgccgct aaataatccc gctgtccgcc
    24661 atgcactggc actggcgatt aataaccagc gcctgatgca atccatctat tatggtacgg
    24721 ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa
    24781 ttactgaata caatccggcg aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact
    24841 taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa
    24901 ctgccgaact gattcaggcg gatatggcgc aggttggcgt aaaagtggtg attgtgccgg
    24961 tagaaggtcg ctttcaggag gcgcggttga tggatatgag ccatgatctg acattatccg
    25021 gttgggcgac ggacagtaac gacccggaca gtttcttccg tcctttactg agctgcgcgg
    25081 caattcattc acagaccaac ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc
    25141 gtaaggcgct ctcctcgcag cagctggcgg cgcgtattga agcctatgac gaagcgcaga
    25201 gtattctggc gcaggaatta cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct
    25261 atcggtacga tatcaaaggt ctggtactta gcccgtttgg taacgcctcc tttgctgggg
    25321 tgtatcgcga gaaacaggat gaggtgaaaa aaccatgatt atcttcacct tacgccgcat
    25381 tttgttattg attgtcacct tgttcctgct gaccttcgtt ggcttcagct tgagctattt
    25441 cacccctcat gcgccgttac aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa
    25501 cggcctgatc cactgggatt ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca
    25561 gttaaaagag gtcttcccgg cgacgatgga actgtgcatc ctcgccttcg gttttgcgct
    25621 gattgttggc atccccgtgg ggatgattgc cgggattacg cgccataagt ggcaggataa
    25681 cttgatcaac gccatcgcct tactgggctt ttcaatcccg gtgttctggc tggcgcttct
    25741 gttgacgctg ttttgttcac tcacgctagg ttggttgccc gtttcagggc gtttcgatct
    25801 gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt gatgcctggc tttcggactc
    25861 accatggcgg gatgaaatga tcatgagcgc aatccgccat atgatattgc ccgtgatcac
    25921 cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt
    25981 gtatgaccag aactatgtaa aagcggcggc gacccgcggt ttgtcgcgct ttactatttt
    26041 gcgtcgccac gttctgcata acgcgttacc gccggttatt cctcgcctgg gcttacagtt
    26101 ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt tttagctggc cgggtttagg
    26161 gcgctggtta attaacgcca tccgccagca ggattacgca gccatttccg ccggagtgat
    26221 ggtgtgtggc tcactggtca ttattgttaa cgtgatttct gatattttgg gtgccatggc
    26281 taaccctctg aaacataagg aatggtatgc cttacgatag cgtatacagc gaaaagcgcc
    26341 cgccgggcac gctgcgtacc gcctggcgca aattttatag tgacgcctct gcgatggtcg
    26401 gcctgtacgg ctgcgcgggg ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt
    26461 acggcatcga ccagcaattt ctcggttatc aattattgcc gccatcatgg tcgcgctatg
    26521 gcgaagtttc tttcttcctg gggactgacg acctcggacg cgatgtttta agccgtttac
    26581 tgagcggagc tgcgccaacc gtgggtggcg catttgtggt gacgcttgcc gcgacgatct
    26641 gtggcctggt actgggcacc ttcgccgggg cgacacacgg cctccgctca gcggtgctta
    26701 accatattct ggatacctta ctggcgatcc cttcgctgct gttggcaatt atcgttgtag
    26761 cgtttgccgg accgagtttg tcccacgcta tgtttgctgt ctggctggca ttgctgccgc
    26821 gtatggtacg ttcgatttac agcatggtgc atgacgaact ggaaaaagag tacgttatcg
    26881 ccgcccgtct ggatggcgca tcaacgctga atattctctg gtttgctgtg atgccaaaca
    26941 tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg
    27001 ccgcgctggg ctttctcgat ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc
    27061 tcggtgatgc gctggaactg atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg
    27121 caattatgat tagcgtgttg ttggttaacc tccttggcga tggcgttcgt cgtgcgatta
    27181 ttgcgggggt ggaataatgc cattactcga tattcgtaac ctgaccattg aatttaaaac
    27241 cggtgatgag tgggttaaag ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat
    27301 ccgcggtctt gttggtgaat ccggttccgg caagagtttg attgcgaaag cgatttgtgg
    27361 ggtgaataaa gataactggc gtgttactgc tgaccgtatg cgttttgatg atatcgattt
    27421 gctgcgtctc tccgcacgcg aacggcgcaa actggttggc cataacgtgt cgatgatttt
    27481 ccaggaaccg cagtcgtgtc ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa
    27541 catcccagcc tggacctata aaggccgttg gtggcagcgt tttggctggc gcaaacgccg
    27601 tgccattgaa ctgctgcacc gcgtggggat taaagatcac aaagatgcga tgcgcagttt
    27661 tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg atagccattg cactggcgaa
    27721 tcaaccgcgt ctgctgattg ctgacgaacc gaccaactca atggagccaa caacccaggc
    27781 gcaaatcttt cgcctgctga cgcgtctcaa ccaaaacagc aataccacta ttttgcttat
    27841 cagccatgac ttacaaatgc ttagccaatg ggcggataaa attaacgtgc tttactgcgg
    27901 tcaaacagtg gaaaccgcgc cgagtaagga gttggtgacg atgccacatc atccttatac
    27961 ccaggcgctg atccgcgcga taccagactt cggcagcgcg atgccgcata aaagtcgcct
    28021 caatacgctg cccggcgcta tcccactgct ggaacagtta ccgattggtt gtcgtctggg
    28081 gccacgttgc ccgtatgcac aacgagaatg cattgtgacg ccacgtttga cgggggcgaa
    28141 aaatcatctc tatgcctgtc atttcccgct gaacatggag aaagagtgag atgatcgaaa
    28201 cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc
    28261 gtcagaccgt agaagcggta aaacccttga gctttacgct acgtgaaggc cagacactgg
    28321 cgattattgg cgagaatggt tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga
    28381 tagagcctac cagcggcgag ttattgattg acgatcatcc actgcatttt ggcgattatt
    28441 ccttccgcag tcagcgtatt cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc
    28501 gtcaacgtat ctcgcaaatt ctggattttc cactgcgcct gaacaccgac ctggaaccgg
    28561 aacagcgtcg caaacagatt attgaaacga tgcgtatggt tgggctgcta ccggatcacg
    28621 tcagttacta tccgcatatg ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg
    28681 cgctaatatt gcgcccaaaa gtcattattg ccgatgaagc cctcgcttca ctggatatgt
    28741 cgatgcgttc gcagttgatt aatctgatgc tggagttaca ggaaaaacag ggcatttcgt
    28801 atatttatgt tacccagcat atcggaatga tgaagcacat tagcgaccag gtgctggtga
    28861 tgcatcaggg cgaggttgtc gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc
    28921 atgaactcac caaacggctg attgccgggc actttggcga agcattgaca gcggatgcgt
    28981 ggcgtaaaga tcgctaattt tctgaatcct ccttccagat tatccacctc attctgacaa
    29041 gagattaaat cttgtcagaa tgaggtgatg gataaaataa aatcaaatgc gcgtgattta
    29101 agacgcaatc tcactttgca ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt
    29161 ggtgatttca aatttcgccg tcaacatccg gtggggagct acattctcga ttttgcttgc
    29221 tgctcggcgc gcgtagtcgt tgagctggat ggtgggcagc atgatttggc agttgcctat
    29281 gataccaggc gcactagctg gcttgagtcg cagggctgga ccgtgctgcg tttctggaat
    29341 aacgagattg attgtaatga ggaggcggtg ccggagatta ttctgcagga actgaaccgc
    29401 cgatcaccct caccctgaaa gggcgagggg gccgaccgag ccgaatagct gttgtggtga
    29461 aaacatggag acagtgctgg agaatattcg gcaaggtctg aaccatccca gccatcgcca
    29521 taaaagggtt aggggctgta tgagcctgtt tgttgctggg gtaacaatat ttgcacaata
    29581 tggtcccctc gcccctctgg ggagagggtt agggtaaggg gaaaagcgcc caccctgccg
    29641 cagcctgctc cggtcggacc tggcaactat agctactcac agccaggttg attataataa
    29701 ccgtttatct gttcgtactg tttactaaaa cgacgaatcg cctgattttc aggcacaaca
    29761 agcatcaaca ataaggatta aagctatggg ttttctttcc ggtaagcgca ttctggtaac
    29821 cggtgttgcc agcaaactat ccatcgccta cggtatcgct caggcgatgc accgcgaagg
    29881 agctgaactg gcattcacct accagaacga caaactgaaa ggccgcgtag aagaatttgc
    29941 cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga
    30001 caccatgttc gctgaactgg ggaaagtttg gccgaaattt gacggtttcg tacactctat
    30061 tggttttgca cctggcgatc agctggatgg tgactatgtt aacgccgtta cccgtgaagg
    30121 cttcaaaatt gcccacgaca tcagctccta cagcttcgtt gcaatggcaa aagcttgccg
    30181 ctccatgctg aatccgggtt ctgccctgct gaccctttcc taccttggcg ctgagcgcgc
    30241 tatcccgaac tacaacgtta tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta
    30301 tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac gccatctctg ctggtccgat
    30361 ccgtactctg gcggcttccg gtatcaaaga cttccgcaaa atgctggctc attgcgaagc
    30421 cgttaccccg attcgccgta ccgttactat tgaagatgtg ggtaactccg cggcattcct
    30481 gtgctccgat ctctctgccg gtatctccgg tgaagtggtc cacgttgacg gcggtttcag
    30541 catcgctgca atgaacgaac tcgaattgaa ataatcattc tgttggtaaa tattgggcgg
    30601 cgttctgccg cccgttatct ctgttatacc tttctgatat ttgttatcgc cgatccgtct
    30661 ttctcccctt cccgccttgc gtcaggataa cgatttcctt tacgaccaag gagcgcccat
    30721 ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc
    30781 ggagtatgac gttctgcctc tggtcccgga agccgcaaag gtcagcgatc cctttctgct
    30841 cgacgtgatc cttgatgaag aaacgctggc tcccttcctt tcatggctgg tccctgcgcg
    30901 cgttcttgca gtggaattgt tccctgacca gcttaccgtg acccgttcac agaccttcac
    30961 cgcttatgaa cgcttgtcta cggccctgac ggttgctcag gtttgcggcg tccagcggtt
    31021 atgtaactac tattcggcgc gacttacgcc gctccccggg cctgattcct ccagggaaag
    31081 caatcatagg ttggcacaaa tcacgcaata tgcccgccaa ctggctagct cgccttctat
    31141 tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt cttactgcct gggactgtgt
    31201 gatcattaac caaatcattg gttttattgg ctttcaggcg cggaccattg cgacatttca
    31261 ggcttatctc gggcacccgg tacgctggtt acccggtctg gaaatacaaa actacgccga
    31321 cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc agctatgagg tggaaaaact
    31381 acctgaagag tacacaaaaa gttcaacagc agaactttgc caactggccg aaacactctc
    31441 tctccaccct atttcacttt cccttctcga aaggttgtta aacagtacac gggttaatac
    31501 acagccggat aataagcttg cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg
    31561 ttttgccgcc tgtatggatt catcaaatga atataaaaaa tcagccccct tctgcgcaag
    31621 ggcgaaaatg aaattaacca atgggctgac cgtcattctg ttgagcacgc taccgttcag
    31681 gcgatacaat ggctgacccg agcacccgat cgctttagcg ccgcccagtt cagcccttta
    31741 ctcggacacg aaaaatcatc aacgcagatt attaatctgc tggtatggag cgggctgtgt
    31801 ggctggataa atcgcttaaa aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga
    31861 cagattcgcg taaaactgtc agccgctcta atggccacca aaatagacaa ttatgtttca
    31921 ggacaacccg ctgctagcgc agcttaaaca gcaactgcat tcccagacgc cacgcgctga
    31981 aggggtggta aaagccacag aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag
    32041 ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt
    32101 gatccacagt gaaaaagaac gtgaatccgc agagccagaa gaactggttg aaccgttcct
    32161 gactcgtttc gtgggtaaag ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca
    32221 tccactctta aaagacgcca ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa
    32281 agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt
    32341 ctatgctgaa ctgacacaat acatcacttt tggtgacgac cactttgtac cgtggtgggt
    32401 tacccttgcg cgccataatc tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct
    32461 cgatgaaggt ctggttcgtg aagatctgac cgcgctggat tttgtcacca tcgacagtgc
    32521 cagcacagaa gatatggatg acgccctttt cgctaaggcg ttgccggatg acaaacttca
    32581 gctgattgtg gcgattgccg atccaaccgc gtggattgct gaaggcagca agctggacaa
    32641 agccgcgaaa attcgcgcat tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc
    32701 tcgcgagctt tctgacgatc tctgctcact gcgcgccaat gaagtccgcc cggtactggc
    32761 atgccgcatg acgctctccg ctgatggcac cattgaagat aatatcgaat tctttgccgc
    32821 caccatcgaa tccaaagcga agctggtgta tgaccaggtt tctgactggc tggaaaatac
    32881 cggtgactgg cagcctgaaa gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat
    32941 ttgccaacgc cgcggcgagt ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga
    33001 ttaccgcttt attctcggtg aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg
    33061 cattgccaac cgtatcgtcg aagaagcgat gattgccgct aacatttgtg cggcccgcgt
    33121 tctgcgcgat aagctcggtt ttggcatcta taacgtgcat atgggctttg atccggcgaa
    33181 tgccgacgcg ctggcagcgt tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt
    33241 gctcacgctg gacggtttct gcaaactgcg tcgtgaactg gatgcgcaac caactggttt
    33301 cctcgacagc cgcattcgtc gcttccagtc atttgctgaa attagcactg aacccggtcc
    33361 tcactttggc ctcggtctgg aagcatacgc cacctggact tcgccgatcc gtaaatatgg
    33421 cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc
    33481 gcaggatgaa atcactgtcc aaatggccga acgtcgccgt ctcaaccgga tggcagaacg
    33541 tgatgttggc gactggttat acgcacgctt cctgaaagat aaagccggga ccggcacccg
    33601 tttcgcggcg gaaattgtcg atatcagccg tggcggcatg cgtgtgcgtt tggttgataa
    33661 cggcgctatc gcctttattc cggcaccttt cttacacgct gtgcgcgatg aactggtttg
    33721 cagccaggaa aacggcactg tacaaattaa aggtgaaacg gtttacaaag taactgacgt
    33781 tattgacgtc accattgccg aagtccgcat ggaaacccgc ggcattattg cgcgcccggt
    33841 cgcgtaatct cctttcacgg cccattcctc atggatgggc cgtttatttc cccgctctac
    33901 cttcgttata tccaggcaga ttttttaact gactttcgtt tgaaaactgg cgtttttcca
    33961 aaactggatt acaaataaat atataaaaaa acaatgcgtt tagccgccgt taatttcatg
    34021 taagtcatgt tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat
    34081 catgaaaacc gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata
    34141 ctggcggttg acggaaagca gcgatgtttt gcgctttttt accaccgaaa ccacagaacc
    34201 tgatcgtact ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat
    34261 cacctccagc ctgatgatga gtctgaccgt cgatgagagc gatctttctg tgcatctggt
    34321 aggacgaaaa atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc
    34381 ggcagttgct cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc
    34441 acattccgca atagtgattc tcgacagccg ggggaatatc caacgcttca atcggttatg
    34501 tgaagattac actgggttga aagaacacga cgtcattggc caaagcgtgt ttaaactgtt
    34561 tatgagccgt cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg
    34621 caatgcatat gaagtcgaac tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt
    34681 tcgcaataaa ataaatttgt ccacagcggc agtggcaaaa acgagatttt tttaatctgt
    34741 tccggtaccg acattaccga agagcgccgc gctcaggagc gactgcgtat tctggcaaat
    34801 accgacagta tcaccggact gccgaatcgt aacgcaatgc aggagttaat cgatcacgct
    34861 attaatcagg ctgataacaa taaagttggg gttgtgtatc ttgatttgga taattttaaa
    34921 aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt tattacgcga cgtgtcattg
    34981 gctattttaa gctgtctcga acatgaccag gtgttggcgc gtccaggtgg cgatgagttt
    35041 ctggtactgg catccaatac ctcacaaagc gcgctggaag caatggcatc acgaattttg
    35101 acccgcttac gcctcccctt tcgtattggt ttaattgaag tttataccag ctgttcagta
    35161 ggtatttcgc tctctcccga acatggttcg gacagcgcga ctattattcg tcacgccgac
    35221 acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt tactccagaa
    35281 atgaatcagc gggtatttga atatctctgg ctggatacca acttgcgtaa agcactggaa
    35341 aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt
    35401 ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc gttggacttc
    35461 atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt gattctcgat
    35521 gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt ggcggtaaat
    35581 atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca ggttctccag
    35641 gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg tctgattgag
    35701 aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc gcaagtgcat
    35761 ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt tccgatcgat
    35821 gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt ctcgcagtca
    35881 ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt
    35941 gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga gcggcaagga
    36001 tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa acgctatctg
    36061 aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat tatatatcgt
    36121 attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg caatttttgc
    36181 attattcatt ttccactttt gctataagga tgtccttgat gtctgaattt gatgcccagc
    36241 gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat taccactctg
    36301 cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct gaatctacgc
    36361 cagacatccc gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt ttcctatttg
    36421 aggatgcccg gcgcgtcagt atttttttat ttagtattat aacgttataa gaattacagc
    36481 gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc aggttagcgt
    36541 aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg atctcaacac
    36601 cctcgaaaaa ctgagttacc tccgccgtgc acatggcttt gcagtttcgc ttgatagtga
    36661 tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc
    36721 cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggcagca gcaatgccct
    36781 gctcgcccgg actctggggg agcagaagaa aaatgtcact atcatcacgg tcagcagcta
    36841 catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca
    36901 gaaaaaaagc gaaagtatgg ttggcccgtt gacacgccag tgcatccaac aggtgcattt
    36961 cagcaaagca tttattggta ttgatggctg gcaacctgaa actggattca ccggtcgcga
    37021 tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct
    37081 gactgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt
    37141 caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag
    37201 taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg atgatttgca
    37261 gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact atttacctgt
    37321 tattaacacg cttaagatat aaaattatta tcagcgatat aacaggaagt cattatcacc
    37381 tgcgtgatat aaccctgcgc gcgagcagat ttcacggaat aatttcacca ggcttattct
    37441 tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga gagagtatta
    37501 tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattactttg gcaatgtctc
    37561 tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggc gcgggtgcag
    37621 gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag
    37681 ctgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat gtcgattatc
    37741 gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat aatacgcaca
    37801 gtaaaatatt cgaaagccac ggatatatcc gtggcttttt atgttaaccg cctgcgagtt
    37861 ttaccttcat ccctttcgct tcaagtagtg attttaataa atcacgctta tcgccctgga
    37921 tttcaataac tccatcttta actgctccgc cgcagccgca ttttttcttc agttccgctg
    37981 caagttttgt cagttcggca tcatcgagat cgacaccggt aatcaggcaa acgcccttac
    38041 ctttacgtcc actggtctga cgctgaatac gcaccacacc gtcgccttta gggcgtacag
    38101 gggccgcttt gggctcatca atacgtccgg tttccgttga gtagaccaga cggctgttgg
    38161 aatcactcat catgcactcc gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg
    38221 atctaccgat tgcgttaccg ggcgaccaat caccatataa tcaacaccag ccgacaacgc
    38281 ctgttctggc gtcataatgc ggcgctggtc accagcttca ctcccctgcg gacgaatgcc
    38341 cggcgtaacc agtttgaact cctgaccgaa tacctgttta aagcgcacag cttcctgagc
    38401 agaacacacc acaccatcaa ggccacattt ttgcgtcagt gccgccagac gttctgcata
    38461 atctgcaggt gacagtgtca tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa
    38521 cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc
    38581 tgcggtcatc atacgcgccc caccagaggc atgaacattc accatccaca cgcctaagtc
    38641 agctgcagca gcgacagcgt gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag
    38701 aaagatatca aaaccacgct gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa
    38761 catctctttg ccgaccttca gacgacaatc gcgtgggtcg atcttgtcga caaaggacag
    38821 cgcgtcatca cgattatgat aatcaagggc aacaaccaca ggagaattcg taacagcgcg
    38881 ggaagaagat gaagcagtta acgtcatgac cagaccttct tgatgatggg caccggaagt
    38941 ggcgcgaaac aggtaaacgg cgagcattct acccgtggaa ataaaaaatt aacagttgcg
    39001 atttttcccc tggcaggctt tttctgctcc gtcgttgccg gagcacaatg gaacatcaat
    39061 aactagtatg ttgtaactaa agtcggattt ttttaaaaat tacaggccat caagaccgcg
    39121 aatcggttta atggttgacc aggcccgaca agacggacaa tgccagtaga gggtgtatgc
    39181 ggtaaaacca catttctggc agcgataacg aggcttacta cgtacctttt cgccaaccat
    39241 gtcacgcagc accatcaggc tctctttggc acgcccttct tccgcttcat ttaagtggta
    39301 atccattaac ttatggaaca cacgcatggt cggatgacgc tgaagctggc gcgtaatata
    39361 gacctgtgcg gcctcactac cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc
    39421 atcggcaccg gtgttctctt ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt
    39481 acccaactgc tgatagcagg tttgcaacat ttccagcgtt tcgctgacca gttctctgtc
    39541 ctgggatatc acgcgttgca aactttcgac ggctttggcg tattctcctt tcgccataaa
    39601 cacgcgtccc atcatgatgg atacgcgggc gctgttttta tctgccgccg cccctttttt
    39661 tagcaaggtc atggcacgat cgagatcgtc gctggccata tgctgcaggg ctaactcaca
    39721 gtagaaatgg gcaatttcga cgcgctgttt atctttaccc agcttcacca ggcgttcggc
    39781 aacatcaatt gctttctgcc actcgctggt agcctggtag atttgtagca actgttgcag
    39841 cgcgccaatg cggaagtcag tttcatcggt cagctgattg aacatgtctt ccgcgcggtc
    39901 gtataacccg gcggccatgt aatcacgccc cagttgttga atcgccaaca gacgctgttc
    39961 ataggtcagt gaggcgcttt ccattagggt ctgatggatg cgaatagcgc gatcaacttc
    40021 gccacgcgaa cggaacaggt ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc
    40081 tttaagcata tcgagaaaca ggtctaccgc tttatcctgt tgattactaa gcaggaagtt
    40141 aacccccgct acgtaatcac gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc
    40201 acttctgcgg cccatatacc agccataggc agcggctaca ggcaaaagca gaaacaacaa
    40261 ctccagcata gaaagttatt ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt
    40321 cgcgggtgaa agctggtttt ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac
    40381 acgaactcgc agccagaaca ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa
    40441 caataccgcc agcaatgtgg aaatacggta ctccccttgc gctaacagat aattaaacgt
    40501 cacctgttga tcgttctgcg cacccaacgt gaccgaaatc acgaagatcg ctaacaccag
    40561 taagaaaatg agtaaatatt tcacattact tcccgttatg tggttaaagc gaataaatta
    40621 tggtcaactt accgcaatag ccctttaaag taccattttc gcgccgtctg cgaaatggca
    40681 tccacgcgca ttgttgatga tttaagggct aaattcggaa aatcaaccag cgttaacttt
    40741 cttgttctcg ttgcgctatt tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa
    40801 ttcgttgcgc aagccacgtt gccaccgcca ccagcgccca cgaaatcaac gtagctacta
    40861 ccagatcgcg tggccaatgc atcccgagca gcaggcggct tcccatgact cccgttgccc
    40921 agaccagcaa gatagcaatg gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca
    40981 gcagtgccca actggcagca aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt
    41041 tctgccagtg tgaacgcaaa tattgtggga tatttttctc ttcagccaac tgttctttca
    41101 ctagatttcc gcgttctgct cgctttaaag tgtagaactc atcaaccgga atatgatgtg
    41161 ttttttccag ccagataaca aaaggtcgtg gttcctggac tttgtctttg atccaggatt
    41221 taacgccttg tcccacaatg attacggccg ccagaatggc aaataataca aaggcagcct
    41281 taatgcaaaa acgcagacac cagagaaacc agccgaataa aatcaaatgt gtaatgacgc
    41341 cccagggctg ggtgacagtt tcagtaaccc aaaaagccgc ttttagtagc caactttgtt
    41401 ctccaggttg ccaacgccag ccagaaatcc atacggctac tggcatgaca agcaatagtg
    41461 cagctcccac tgcggtacgt ctggcaatcg aacgcatggc ctctcctttt tgataagtcc
    41521 cacaatcata actgaaaacg ccagttccag gaaaaattga cagatttgtg ccattccgtg
    41581 aacgatcgac gcgtcgtgat taggtgaacc ccttctcgtt atggcaaaat aagccaatac
    41641 agaaccagca ttatctggag aatttcatgc agcttaaacg tgtggcagaa gccaaactgc
    41701 caaccccatg gggcgatttc ctgatggtgg gatttgaaga actggcaacc ggacacgatc
    41761 atgtcgcgct agtctatggc gatatttccg ggcatacccc ggtacttgcg cgcgtccatt
    41821 ccgaatgtct gaccggtgac gccctgttca gcttgcgctg cgattgtggc ttccagctcg
    41881 aagcggcatt gacgcaaatt gccgaggaag gccgtggtat tttgctgtat caccgtcagg
    41941 aaggtcgtaa cattggtctg ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt
    42001 acgataccgt agaggctaac catcagttag gcttcgccgc tgatgagcgc gacttcactc
    42061 tttgcgctga tatgttcaaa ctccttggcg tcaatgaagt ccgcttgtta accaataacc
    42121 cgaaaaaagt cgaaattctg accgaagcag ggattaatat tgttgaacgc gtaccattga
    42181 ttgtaggtcg taaccccaat aacgaacatt atctcgatac caaagccgag aaaatgggtc
    42241 atttgctgaa caaataaccc tctcgcattg tggaattaat ttgcttgccg gaagcaaaat
    42301 aaccggcaag caaatatttg ttacttcagc atattacgaa tgacataatg caaaatgccg
    42361 tcgttctggt agtaggtcaa ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc
    42421 tcctggctac catccgcgcg cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt
    42481 tgcagatcgc caatatcaat cttctcttcc ccggttagtc ctaacgtttt acgcgttacg
    42541 ccttgcggaa attccagcgg caggatgccc atgccaatta aattcgaacg gtgaattcgt
    42601 tcaaacgatt cggcaatcac cacacgaatt ccaagcagac gaggaccttt tgccgcccag
    42661 tcacggcttg agcctgaacc atactctttc ccggcaatca ccgccagcgg cgtttgttcc
    42721 tgcttatagc gcatcgcagc atcataaata gagactacgt cgctgtcagg taaatgccgc
    42781 gtcatccccc cttcaacgcc aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg
    42841 ccgcgcatca tcacttcatg gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc
    42901 tcaacacccc gaccttgtag atatcgaccc gctgggctgt cgggcttaat actgcccgcc
    42961 ggagagatat ggtcagtggt gactgaatcc cccagcattg cgaggatccg cgcaccgtga
    43021 atatcttcca ctggtgctgg tgttgcctgc atttcatcaa agaaaggcga taagcgaata
    43081 taggttgagt cctcctgcca accgtaggta tcggatcgtg tgacgttaat ttccttccac
    43141 tctgctgtgc cttcaaaaac ttctgcgtac tctttgcgga acatttctgt ggagacttgt
    43201 tctaccgcac gggcaatttc ttgtgccgat ggccagatat ctttcagata aaccggatcg
    43261 cctttacgat catgaccaat aggctcagaa gccaggttga tattcatatt tcccgccagc
    43321 gcataggcaa ccaccagcgg cggcgaggcc agccagttgg ttttaaccag cggatggata
    43381 cggccttcaa agttacggtt gccggacagc actgcaccga cggttaaatc gccttttttg
    43441 attgccgttt cgataggatc gggcagcggc ccagagttac caatacaggt agtacaaccg
    43501 tatcccacaa ggttaaaccc cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga
    43561 tagtcagaaa caactttcga gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg
    43621 aggccaagaa ttacggcttt tttcgccagc aagcctgcag ccatcagcac acttgggtta
    43681 gaggtgttgg tgcacgaggt tatcgcagca atgaccacag cgccatcagg taactgatac
    43741 tgatgtccgt tcataacata atcgaccggc tggcgatctt tatgcgtggc attcacttcc
    43801 agttcgttac tggcggcaaa tacttttggt acatcgggca gtgcaacgcg atcctgtggg
    43861 cgttttggcc ctgccaggct cgcttcaacg tcattcatat ccagttctaa cgtactggta
    43921 aaaatcggtt catcgcccgg gttacgccac atgccctgcg ctttggcata tttttcgacc
    43981 aactcaacct gatcttcgct gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg
    44041 attgggaaga agccacaggt ggcaccatat tctggcgaca tattggcaat ggtggcgcga
    44101 tccgccagcg gtagtgaatc cagaccatca ccataaaatt cgacgaattt ctccaccacg
    44161 ccatgtttgc gcagcatttg ggtaacagtg agaaccaggt ctgtggcggt aataccttca
    44221 cgtaatttac cggtaagttt aaagcccact acgtccggga taagcatgga aaccggctgg
    44281 cctaacattg cggcttctgc ttcgatccca cccacgcccc accccagcac gccaaggccg
    44341 ttgatcatag tggtgtgcga gtcagtacca acgagtgtat ccggataagc aatccattca
    44401 ccgtcctgca attcactcca cactgctttg ccgagatatt cgaggttaac ctgatggcaa
    44461 atgcctgtgc ctggcggcac gacgctaaac cgactgaacg cttgctttcc ccatttcagg
    44521 aacacataac gttcgtggtt gcgctccatt tccaggcgta cgttttcttc aaatgcctca
    44581 tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc
    44641 gggttaacct ttgcagtatc gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga
    44701 tcaacaacgg caggtacgcc ggtaaagtcc tgcatcaaca cccttgccgg gcggtaggca
    44761 atttcacggt cagcatgggc atttttcagc catcctgcca gcgcgtggat atcctcttcg
    44821 gtaaccgagt taccatcctg ccagcgcagc aggttttcga gcaaaacttt gagtgacttg
    44881 ggtagacggg tgatatcgcc cagtgattta gcagcaagcg gcaggctgta gtagtggtaa
    44941 gttttatctt tggcctgcaa cgtgtcctta ctggcttctc gtagggttga cgacatagct
    45001 cctccttaat gacagggttg cgaagccctg attctcttca gggtgaatat taaagataac
    45061 acaaacatcg cgtaacgatt tgataacaac ccaaattgat aaaagaggag aaaccttgtg
    45121 gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag
    45181 gagttcggcc cagaagagca tcgacagaga aaacgcgccc atccaggacc aatatttcat
    45241 atttgtgttg atcataggct acacccttaa cggttattat tttgtgttga gataactcaa
    45301 gctgttattg ctgcggtgta ttaaaagccc tttaattcat taaaaatggg taataagaat
    45361 gatgataatt attgtttcgg ctgatcatct tcagcaaaca gttctataaa ggcttgctgc
    45421 tgcgatgtta gttgccagtc gtctggaata acgacggaac attcccgttt cgtttctgtt
    45481 tcattgtcac tatgctggca taatggtgac gactccagtt gctcctgacc gaccatagtt
    45541 atccccacac tttccaaatc agcagtgcga caaccaccca gaaaagtgca gaaccaagaa
    45601 aaaccgccag ccaggcttta cgttttaatt caggatcgcg gcggggttcc tgatttccag
    45661 aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg aacgattggt
    45721 gtaattaatc atacgatgag acaaatccta aagaaacttt agt
//
LOCUS       DAJTVE010000014        45669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000014 DAJTVE010000000
VERSION     DAJTVE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(132..746)
                     /locus_tag="OO439_000777"
     CDS             complement(132..746)
                     /locus_tag="OO439_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315223.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVVLSGSQWQSLPVLPAD
                     GTQVSFPYAGEWLTEEEIRAVFDAMRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQ
                     TRRFRLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGL
                     ACDVLRIPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            complement(791..1225)
                     /locus_tag="OO439_000778"
     CDS             complement(791..1225)
                     /locus_tag="OO439_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCR6315224.1"
                     /translation="MYGTCETLCRELAAQYPGDAPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQARTLLKNNTREND"
     gene            complement(1239..1460)
                     /locus_tag="OO439_000779"
     CDS             complement(1239..1460)
                     /locus_tag="OO439_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315225.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(1461..2144)
                     /locus_tag="OO439_000780"
     CDS             complement(1461..2144)
                     /locus_tag="OO439_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6315226.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(2221..2532)
                     /locus_tag="OO439_000781"
     CDS             complement(2221..2532)
                     /locus_tag="OO439_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315227.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2529..3455)
                     /locus_tag="OO439_000782"
                     /pseudo
     CDS             complement(2529..3455)
                     /locus_tag="OO439_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            3849..4097
                     /locus_tag="OO439_000783"
     CDS             3849..4097
                     /locus_tag="OO439_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6315228.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            4094..4531
                     /gene="umuD"
                     /locus_tag="OO439_000784"
     CDS             4094..4531
                     /gene="umuD"
                     /locus_tag="OO439_000784"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6315229.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            4531..5805
                     /locus_tag="OO439_000785"
     CDS             4531..5805
                     /locus_tag="OO439_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR6315230.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(5807..6223)
                     /locus_tag="OO439_000786"
     CDS             complement(5807..6223)
                     /locus_tag="OO439_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6315231.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6216..7196)
                     /locus_tag="OO439_000787"
     CDS             complement(6216..7196)
                     /locus_tag="OO439_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6315232.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(7610..7918)
                     /locus_tag="OO439_000788"
     CDS             complement(7610..7918)
                     /locus_tag="OO439_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCR6315233.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(8005..8649)
                     /locus_tag="OO439_000789"
     CDS             complement(8005..8649)
                     /locus_tag="OO439_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR6315234.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(8829..9608)
                     /locus_tag="OO439_000790"
     CDS             complement(8829..9608)
                     /locus_tag="OO439_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCR6315235.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPVGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEIYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(9610..10023)
                     /locus_tag="OO439_000791"
     CDS             complement(9610..10023)
                     /locus_tag="OO439_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315236.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            10546..11409
                     /locus_tag="OO439_000792"
     CDS             10546..11409
                     /locus_tag="OO439_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315237.1"
                     /translation="MKDQRIELRLPQQQLDELDNFINNIDGQYKPSRSDVLRSFIAQG
                     VRGKFTPASQEAEMFPLSARLNIFFQLCQLLRMECGKDGRSVQPINPTYGYNNRVAST
                     VTAEALVRQVYLQRMTWFFELDAVHLQAINPNLGQDMIVSLMNPQPSPVICNTLDSVI
                     ALRDMFSNIRMVLASAEKTVNDWNDQKTRDALARIQGYVEDNGLQLTFKGYPDTEDYA
                     LQIDMWSLLNWIDNGQGDHRIGDYGLRNDKDLTDKYAVMLEVYQNIRSNHQFDLNGLE
                     QMVKSRQFHMI"
     gene            complement(11434..12933)
                     /locus_tag="OO439_000793"
     CDS             complement(11434..12933)
                     /locus_tag="OO439_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCR6315238.1"
                     /translation="MNVEESEQRWVCACCIGEEFLRHKVEKEGRIQTCDYCDEIHTCF
                     SLEEVCDLTEKAIEAHFYRTDTEPNDMEYASLRHIDGYEWFREGENVVQLIEDLLQSR
                     RALADDIQQLLEYRHSDFDSDVMGLETEFARESCYAERKQISTGRLDSMWINFVTSLK
                     TESRFINNAVSSTLDGIFQGVETMRARGKEPVIIDAGPGTAIPSLYRARWCRNHHELE
                     QILVTPDKELGPPPHQFSGSNRMSAKGISVFYGASSVDTAISEIRPPVGCKVVCAKFS
                     LIRSLRLLNLPALESVLESGSLLDPDYIKRREQAAFLSTFTSRIVEPVLPGEEDFSYI
                     PTQVIAEYLADPLRFNLDGILYPSVQLPGADQEERYNVVLFHKASRVAFMTLPAKDDC
                     MIRYGHQYAEDEWETDICVTEVISAQEDPPPARHDLSDLSLPDDPREPALEIDLDSVS
                     VHGIRAARFEYSTDAVRRSTYHYTPRTSEPAGTITQLWDTASGHDDFPF"
     gene            13201..13455
                     /locus_tag="OO439_000794"
     CDS             13201..13455
                     /locus_tag="OO439_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315239.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDSQSARCLNELRASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            13455..14045
                     /locus_tag="OO439_000795"
     CDS             13455..14045
                     /locus_tag="OO439_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315240.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            14258..15310
                     /locus_tag="OO439_000796"
     CDS             14258..15310
                     /locus_tag="OO439_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315241.1"
                     /translation="MKDTITPLNYTKEAIKDLSVMEKGLRSNAPLTSYIMRQVIDMLD
                     SSVKFILPNCCDIIEPEEYRQTHFDLARLPYPVVTFEIPWFKDNVETQIGDFNISPSS
                     RRIALCWEARQSFEPIPGCNSILNTYIDGGVFILPVSWSDDLKIWILGVGGMFFPYNN
                     KLTKYEPDRTLPASRLVIDTLKENGVAKYNAAHFKAEPFITSMEFKDDLIKQVGSIER
                     LYAQIIMDTRDELQAFIQACSVLNCENVCPVTLSTKPERKFINGRKVQPPEKNKRPSY
                     TYKVLQLSETKVQSNHTGTGKSGGTKRMHLRRGHIRRKNNKLIWIRPAMINANSRAGI
                     VDKDYQINIRKEENKP"
     gene            15486..16088
                     /locus_tag="OO439_000797"
     CDS             15486..16088
                     /locus_tag="OO439_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCR6315242.1"
                     /translation="MGKYQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSHRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            16098..16334
                     /locus_tag="OO439_000798"
     CDS             16098..16334
                     /locus_tag="OO439_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315243.1"
                     /translation="MTGGHYVSKDSPDAKQELLECLLSGKRMTGRDEMDIKRILKTKE
                     ALDNIRNFIDKQQLTNSDTPKKSTVPNEDKKGIR"
     gene            16334..16600
                     /locus_tag="OO439_000799"
     CDS             16334..16600
                     /locus_tag="OO439_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315244.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNKANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(16791..17066)
                     /locus_tag="OO439_000800"
     CDS             complement(16791..17066)
                     /locus_tag="OO439_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315245.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIHKKNRYASYDLRREIEMQHEEFQKMTEREKVPLLKSPE"
     gene            complement(17172..17447)
                     /locus_tag="OO439_000801"
     CDS             complement(17172..17447)
                     /locus_tag="OO439_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6315246.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(17447..17731)
                     /locus_tag="OO439_000802"
     CDS             complement(17447..17731)
                     /locus_tag="OO439_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6315247.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(18630..19508)
                     /gene="repA"
                     /locus_tag="OO439_000803"
     CDS             complement(18630..19508)
                     /gene="repA"
                     /locus_tag="OO439_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6315248.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            20211..20852
                     /locus_tag="OO439_000804"
     CDS             20211..20852
                     /locus_tag="OO439_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCR6315249.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            20993..21655
                     /locus_tag="OO439_000805"
     CDS             20993..21655
                     /locus_tag="OO439_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HCR6315250.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            21922..22542
                     /locus_tag="OO439_000806"
     CDS             21922..22542
                     /locus_tag="OO439_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6315251.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            22569..24263
                     /locus_tag="OO439_000807"
     CDS             22569..24263
                     /locus_tag="OO439_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6315252.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            24269..24586
                     /locus_tag="OO439_000808"
     CDS             24269..24586
                     /locus_tag="OO439_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCR6315253.1"
                     /translation="MPEFFRHLFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKV
                     SYIPATEDINIAIMPFQTADNGLIIVCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNR
                     VQL"
     gene            25264..26334
                     /locus_tag="OO439_000809"
     CDS             25264..26334
                     /locus_tag="OO439_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HCR6315254.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            26338..26775
                     /gene="pilM"
                     /locus_tag="OO439_000810"
     CDS             26338..26775
                     /gene="pilM"
                     /locus_tag="OO439_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCR6315255.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            26807..28426
                     /locus_tag="OO439_000811"
     CDS             26807..28426
                     /locus_tag="OO439_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCR6315256.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            28447..29742
                     /gene="pilO2"
                     /locus_tag="OO439_000812"
     CDS             28447..29742
                     /gene="pilO2"
                     /locus_tag="OO439_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCR6315257.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTIPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            29732..30190
                     /gene="pilP"
                     /locus_tag="OO439_000813"
     CDS             29732..30190
                     /gene="pilP"
                     /locus_tag="OO439_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCR6315258.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            30294..31802
                     /gene="tadA"
                     /locus_tag="OO439_000814"
     CDS             30294..31802
                     /gene="tadA"
                     /locus_tag="OO439_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR6315259.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            31804..32898
                     /locus_tag="OO439_000815"
     CDS             31804..32898
                     /locus_tag="OO439_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCR6315260.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            32960..33496
                     /locus_tag="OO439_000816"
     CDS             32960..33496
                     /locus_tag="OO439_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6315261.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            33541..34026
                     /locus_tag="OO439_000817"
     CDS             33541..34026
                     /locus_tag="OO439_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6315262.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            34042..34668
                     /locus_tag="OO439_000818"
     CDS             34042..34668
                     /locus_tag="OO439_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6315263.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            34686..36047
                     /gene="pilV"
                     /locus_tag="OO439_000819"
     CDS             34686..36047
                     /gene="pilV"
                     /locus_tag="OO439_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCR6315264.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            36520..37341
                     /locus_tag="OO439_000820"
     CDS             36520..37341
                     /locus_tag="OO439_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR6315265.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            37443..38645
                     /locus_tag="OO439_000821"
     CDS             37443..38645
                     /locus_tag="OO439_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCR6315266.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            38749..39207
                     /locus_tag="OO439_000822"
     CDS             38749..39207
                     /locus_tag="OO439_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HCR6315267.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            39204..40040
                     /locus_tag="OO439_000823"
     CDS             39204..40040
                     /locus_tag="OO439_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR6315268.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            40024..41172
                     /gene="traJ"
                     /locus_tag="OO439_000824"
     CDS             40024..41172
                     /gene="traJ"
                     /locus_tag="OO439_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCR6315269.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            41169..41459
                     /gene="icmT"
                     /locus_tag="OO439_000825"
     CDS             41169..41459
                     /gene="icmT"
                     /locus_tag="OO439_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HCR6315270.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            41523..45584
                     /locus_tag="OO439_000826"
     CDS             41523..45584
                     /locus_tag="OO439_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6315271.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
BASE COUNT        11661 a        12269 c        11474 g        10265 t
ORIGIN      
        1 tgaggccaca taactgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc
       61 ctgcggcgac atccttttgc tctttccggg gaccggctgg aaaaggcgga gatttttccc
      121 cgccagcgtc atcatttccg gattttcgcc agtgcatccg ccatgctgcg gatttcagtc
      181 cgggcttcca ggaccacctc ccgcagaata tcccggtcaa accagcgacg ggacggttca
      241 tccggtatac gcagcacatc gcaggccagc ccactggaca ggatatgctc cacacactca
      301 ctgaccagat acatttccac cgacgaaaaa cgagcgcccc ggttcaggat ggcatcgagc
      361 accacgggca ggttttcgtc gtcttcatcg agccagcagg ggtgatcgct ttccttcaca
      421 accagacgaa agcggcgcgt ctggcgggtc agcaacgtct gaccggtggt agtcaacgcc
      481 gcacggatac gccgcgcatc ttctgccacc cggcaactga cactgcatac tgcatcgcgc
      541 atcgcatcaa aaaccgccct gatttcttcc tccgtcagcc attcaccggc atacgggaaa
      601 gacacctgcg ttccgtctgc cggaagtacc ggcagcgact gccactgact gccggacagt
      661 acaacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtggcgacaa
      721 atcagtgcat tcagcgtttc gttcatgatt catggtctcc ctgaatcgcc gcccgcaggc
      781 ggcgataaca tcagtcgttt tcgcgtgtgt tgtttttcag cagggtgcgg gcctgattaa
      841 ccaccgcctg acggcgggtg acgcattccg ggacggcgag gccatgatcc cgggcggccc
      901 attcacgttt ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc agttcagccg
      961 gaacggtcac ctggcggttt tcgctcacgt tgctgataat ctccatcgcc gcggcggaag
     1021 aaataccgga ctcaattcgc tggtcttccg ggatgtcctc caggcgcgcc aggacggtcc
     1081 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctgg atctcttccg
     1141 gggaccagac aaccagcatc agcggcgcgt ctcccggata ctgcgctgcc agctcccggc
     1201 agagcgtttc gcatgttccg tacattctcg tctccttctc aatctttgaa gcaggaatcc
     1261 agttccagaa tttcggtata cgcctccata aacaggcgat tggcgcgata cagcgtctgg
     1321 cgtgtcaggc gttcaacaag cacatcggaa tcaaagcgac agcgatgctc catatcgttc
     1381 agcctggccc gaaggtcaca catggtgttg gccacctccg ccacgtcggc gcgaagtttt
     1441 tcgtgtccgg catagttcat ttacgcagcc tccggttcaa accagttatc attcgcggcc
     1501 ccctgctgca tggcccgttg cacggcggcc agtcgttgct gtccggcacg gtgatactgt
     1561 tcaagcagct cgataccgat ataccggcgt ccggactgga gggcggccac gcaggttgag
     1621 ccgctgcctg caaacgggtc cagcacaatc gcgttcgggt gtgtgaagct ctcaatgagc
     1681 ggttgcaggc tggtaacggg cttttccgtc gggtgatggc gattgcctga atatttccag
     1741 cccagcacgt cgggcaatgg cttctgaggc agcgccggac ggccttttgc caggatgtag
     1801 gcgcattcgt ggcgatagcc cacatatgcg gccttcgatg tgtaggtttt ggtaaacacc
     1861 aggtggccaa ccacgctgaa tcccgcattt ttccaggcgg ccataaagcg atcgacgcgg
     1921 ttccagccgt agaagctgac catcagcgca tcttttttca gcacgcggta catttcatta
     1981 caggccggtt gcagccattc atcggtttta tcgccggcga tggtgcgccc ggaacggtca
     2041 cggaaaccga cgagatacgg cgggtcagtc agtatgaaat caatggcgtt atcaggaata
     2101 cgggccataa catcgatgca gttaccgagg acaaaacggg acataacaac gctccttctc
     2161 ttacagagga caccgggagg gcccccctgt ggtgcccgtt ccccgctgcc tgtgggctgt
     2221 tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctgtttga
     2281 cggcaaggtt atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacaggct
     2341 ggcgtacact gtcaccgacg ccagccggga gcggtaaaag cgcccgtccc gtgcccaccg
     2401 gacgcgggac actgaccaca ggcagcgttt atgccgcgcc gcgtcagccg gcgcagtaaa
     2461 aagaggagca cggcgacatc ctttaccgga aaagaaacgg gcggttgccc gcccgtctcc
     2521 gtcatacttc atccgccata atgcgccaca ttgtcgacta tccacgcagg tccggtgact
     2581 tccggcagct catcgtcatc tgtcggggaa gaccattcca gttcccccca cagcacatca
     2641 acaagctctc cgcgctcata caactgccag ccacagaaat cacagccctg ttcggcgtac
     2701 cagtgctcca gcgtgcagcc gaaaaggcgg cttaattctg caacaacgtc gctttccggc
     2761 tgacaccacg gcgtgtcgaa atctacctga atgaagtttt ccccctgact gctgatattc
     2821 aggtcatacc cacaaggcca cttcacgcca taccgttctg tataccagtg atatgcagaa
     2881 ggaacaccgt tcagaacacc gccgttaaag ccattcactt caatatccag gcgggtcggc
     2941 aaaaccgtca gcaggtcaaa cggcatggtt ttttccggca gtacgttgtc acacagacgg
     3001 ttccaccaca ccgatacatc ctcgttactc cagaagccac accagtcacc tctttttgcg
     3061 gtgaaaagtg cggtgatggt atccctgaca tcgtccggta atccttccca gcgacgctgt
     3121 ccagtaccgc tctgcagtca gagttcatgc agcatcaggc agttttgctc atccagcagt
     3181 acaccgttct gcaggtgtgt cagccagcgg gtgaacgcga tattttccgg tgatacagca
     3241 ccacgtccgg cagccgtcac tccggggcac tgctcagagc ggatattttc agtgatctgc
     3301 agaagtccgg cacttccggc cagaaacagc tgaatacctt catttgtggc gcggcgataa
     3361 aacggtgtga ctgcgccgct ggccagtcgt ttaatctcag cgatctgtgc cggttcacca
     3421 gaaaaataca tacgattcga gcaccagttt ggcatgggaa ttctcctctc acatgcggcc
     3481 tccatcaggc cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag gtgcggcccc
     3541 gaagccgcag cgcgtggagg aggaacgacg aaacgtgacc gggtgaggag gccggcgaca
     3601 gcgacacact tgcatcggat gcagcccggt taacgtgccg gcacggcctg ggtaaccagg
     3661 tattttattc acagaaccgt gcgcaaaatg ctgtggataa gctggacaca gcagtccaca
     3721 gcaggcatac agccacactc tcagagagtt gagcgttttt tagtcacaat aatgacattt
     3781 gactgtgtgt ggattcagtc attagaatgc tgtatatgca tacagcaata gaaaagggag
     3841 atgagaacat gatccgcatt gaaattcttt tcgaccgcca gagcacaaaa aacctcaaat
     3901 caggcacact tcaggcgcta cagaatgaaa tcgaacaacg tctgaaacca cactacccgg
     3961 aaatctggct gcgtatagac cagggcagcg ccccgtctgt atctgtcaca ggagcccgca
     4021 acgacaagga taaagaacgt atcctgtctc tgctggaaga aatctggcag gatgacagct
     4081 ggctgcctgc agcatgagta ccgtttatca ccgtccggct gacccttcag gcgatgacag
     4141 ttacgtgcga ccgttgtttg ccgatcgttg ccaggccggt tttccttcgc ctgccactga
     4201 ttatgctgag caggaactgg atctgaacag ctattgcatc agcagacctg cagccacctt
     4261 ctttctgcgc gccagcggtg aatcgatgaa ccaggctggc gtgcagaatg gtgatctgct
     4321 ggtagtggac agggccgaaa aaccacaaca cggggacatc gttatcgctg agatcgacgg
     4381 tgagttcacc gtcaaacgac tgctgttgcg cccacgcccg gcactggagc cggtttcaga
     4441 cagcccggag ttccgcacac tgtatccgga aaacatctgt atttttggtg ttgtcactca
     4501 cgtgatacac aggacgcggg agttacgctg atgtttgcac tggctgatat caacagtttc
     4561 tacgcctcat gtgaaaaagt tttccgcccg gaccttcgca acgaaccggt catcgtactc
     4621 agcaataacg atggctgtgt gatcgcgcgc agcccggagg caaaagctct tggcatcaga
     4681 atggggcagc cctggtttca ggtgagacag atgcgcctgg agaagaaaat acatgtattt
     4741 tccagcaatt atgcgctgta ccacagcatg agccaacggg tcatggctgt tctggagtcg
     4801 ctttctcccg cagttgagcc ctactcaatt gatgaaatgt ttattgattt gcgggggata
     4861 aatcattgca tctctccgga gttttttggt catcagctca gggaacaggt aaagagctgg
     4921 acaggactca ccatgggggt gggcattgcg cctacaaaaa cgctggctaa aagtgcacag
     4981 tgggcaacaa agcaatggcc acagttttcc ggtgtggtcg cgctgacggc agaaaaccgt
     5041 aatcggatct tgaagctact gggactgcag ccagttggtg aggtctgggg agtaggacgc
     5101 agactgacgg aaaagctgaa tgcgctgggt attaacacag cactgcagct ggcgcaggct
     5161 aacacggcat tcatccggaa aaacttcagc gtcattcttg agcgtacggt acgcgaactc
     5221 aacggcgagt cctgcatatc cctggaagaa gcaccaccgg caaaacagca gattgtctgt
     5281 agtcgcagtt ttggtgaacg aatcacagac aaagatgcca tgcaccaggc tgttgttcag
     5341 tatgcagagc gggccgcaga gaaactacgt ggggagcgtc agtattgccg gcaggtgacg
     5401 acatttgtac ggacatcccc ctttgcagta aaagaaccct gttacagcaa tgccgctgtg
     5461 gaaaagcttc cattgcccac acaggacagc cgggacatta ttgccgccgc atgcagagcc
     5521 ttaaaccatg tctggcgtga aggataccgc tatatgaagg caggagtcat gctggctgat
     5581 ttcacaccat cgggtatagc gcagccggga ttatttgatg aaatccagcc ccgtaaaaac
     5641 agtgaaaagt taatgaaaac actcgatgaa ctgaaccagt cgggaaaagg gaaagtgtgg
     5701 tttgcggggc gaggaaccgc ccctgaatgg caaatgaaac gggaaatgtt gagtcagtgt
     5761 tatacaacta aatggcgaga tattcccctg gccaggctgg gttagttcag tcatctccga
     5821 acatattttc agcgtttctt ctggtctggt cttcaccgtc atttctcgac agactctgct
     5881 ctgtaagagg agtactgcaa tccacagacg tatggagacc agattgtttt tccagtaatt
     5941 caagcaattt cttttcaacc gaaaaagcac ctggtatcac gctggaaatc accttattaa
     6001 tatcatccag agtcgtattt tctgtcagta actccgatat cagcaaagga agacgtttat
     6061 ctatagctgc aaacgccgca ccagtaacta acatcgcgcg catgaaacgt ccccgctcct
     6121 tcagtttttc atttgcaatg agctcatctg ctatcttatc tcctgtccgt tcatccggga
     6181 aaagttcaaa tgagcagcgt ctggatttat tttcatcact cattagtcac accacctttc
     6241 atttttatca ttcccatgac taaatcaaac tgcggggatg agctcagata aaagcgctca
     6301 tcaggaacct tagtcaggtt cttcacagcc tcggccacaa tttcagcccc accaccaaca
     6361 cacattacat gtgtatagtc ggtgaaacgg ctgatgacat cattaactcg tttaataagc
     6421 aacttctgcc gttcattgat aacattaatg atagactcac gctgttcagc attataaatg
     6481 cgacgggaaa ggtagtcagg ttcattacgg tgcacaatga tattgtcagc ctggaatgag
     6541 ctcactcttg tattagcatg tacagccatc tgttccttaa ccccggatgt aatcagggac
     6601 acgccgatat ttggatcaca ccaggttttt gtaatccctg tcattttgct acgaacatgc
     6661 gacacatcaa gagttgtacc accgagatct acgatcaaaa gggattcatc atcctcaagc
     6721 cccgcaagaa cactgaaacc agcgggaata ctttcaggaa gaacggaaac actgcgaata
     6781 acaaatgcat cactcccctg cacccgtact tcacgcatca catttttttt ctttctttca
     6841 atgttttgct tatttttctg attgttggcg tcaaggtatt cagagatcgg caatgtgacg
     6901 atgacatcaa caggctgcgc tttcaggccg gtctgctgta gcgcatgctg aatggccaca
     6961 acattcacat cactgtactg atagcgtgtt tcagtcgtta cgacagcatc agcacttaat
     7021 ggatcaaatg aaaacttctc gccgtcgatc tcgtaattag ccggggcagc atcatccagt
     7081 aaactaaaag accattccgg tttaaagctg ttagggctga ttaacgtttt cacgtcacca
     7141 tcctccaacc aggcaagttt aatgttggta gaaccatcat caataaagat tttcataacc
     7201 agtctccgtt gctcataaac aaatagaaaa aacaaacatg cttcatacat gcaatgtata
     7261 ttcaattata atgaaaaaac ggtctttctg aaaggataaa tgcaacataa aaacacgata
     7321 aattgcatgt atattgtaca cacaacttta agaccagagg aaatcataga tacaaaaaca
     7381 ttatgaaggt tatacgtgat acataaatgc aatataaaaa catcgtggac atcatggttc
     7441 aggttagggg aaaaggaatg aaatggtgaa actgagagga aaggaaagcg gtgacgaatc
     7501 atgtcaccat aaacagaaag atgtctaatg gggactcttt aggtacgtga agaatcccca
     7561 taacgcattt aaaagacaca catcggatac ccttaaggta tccgatagtt tattgaccta
     7621 ttgcaccatc acgttcaagc ttctctctta tagcctcaat aatatatgag ctaaaatcca
     7681 gagatgtttt actggtcgct ttaagttccg catgagcttc aaaataggac agcggtatgg
     7741 ccttaagatt cttagccttc gtcctggcag ccacgggacg acctgaaact tcctctgcac
     7801 cttcatttgg ctgagacggg gctgttgctg ttacggcaga agaagaagtt tgtaatctgg
     7861 cagaatccac caggtttttc agtttttcag cctgtgcact aaatttcgct ttgctcatga
     7921 gataacccag gatgtttgtt agatacttaa aagatatcca ttaaaagccc aaaggatact
     7981 atatggatac ccaatagaca caccttactg tttgttattt tcaatcagat gattgatttc
     8041 ttcgatcagg gcaataactt ccttacctcc tgccgaatag cgcccatgaa cagactcagt
     8101 gattcccatc ccagtctcaa tgacatcctc aaattctgca cgagcagaaa tgcggctctt
     8161 catcagcttc agatgtttga acgatggcag gattgcatca agcttaggga atttcttctt
     8221 gttaggatta actttacaga gatacaggtg agcagttata tcggtcccca tgatagagct
     8281 gatttcgtcc agtactccat caaaatgcat caaaccaata cgttcagtca gggagtcttt
     8341 tgaagggaca ataacgcagt ctgcaaaggc aatcgcagta cgggtaagat ctgagtcaaa
     8401 tcccccacag tcaataagta acgtcttatc gctgttcttg tagggagtca taatttcgat
     8461 taactcatcc actgttttgg gtactttcac ggaaatttct tttccttcag gtctcagccc
     8521 cagaatgata gaaagcccgg tatgagtatc cagatcaact gtatcagact tcatttcacc
     8581 aacaatgttt acggatgaag ttgtcttacc cacaccacct ttgggatgcg tattagcaat
     8641 aacaggcata gtgtatctcc agaatccaca tgtaataccc ataaagaccc cataagacac
     8701 ctttcggata tccaagaggt actaaataga tgttttttta ctctatgccg gtcctgcatg
     8761 tcaacagaat atgaaatacc gataatatat ccatgagata tccgaaaggg atttaaaaaa
     8821 gtcagagctt atggaattct tttgagcata gacaccgcat cggactcagg catcgaaaac
     8881 tgcacccggt gccgtgcagc cacatccagt gcaaacacct tcgtgtagat ctccgttgag
     8941 ctgatggact tatgccccat caggctctgc aggaccttca gcggtatacc ggcatacagc
     9001 atgtgcatcg cataggaatg gcggaacgta tgtggcgtga ccgggacaga gaacgtcaca
     9061 ccatcagcgg cagcggcggc aaccgcctcc ccaatccagg tcctgaccgt tctgtccgtc
     9121 acttcccaga tccgcgcttt ctctgtcctg cctgttcgtt tattacgccg ttccatgggg
     9181 attttcagtg tggccaccat cgtctgcagc tggctgacgt accaggcgtc ggagagcgga
     9241 accagacggt gagtctgctg accgacgggc atccttcctg ccgtcctggc ggctttttct
     9301 gtccgctgct tcagagtggc cagctgcaca aacggatacg ggggcgcaag cgaaaaatcc
     9361 ccccgcgtca gcgccagtgc ttcattaatg cgcgcaccgg tgttccacag tgtggccagc
     9421 agcattttgc ggcgcagatc cggcacgtaa tggagcaggg cgctcacttc aggggccagc
     9481 aggtattttg gcagttcatc atgaaccatc gacatctggc ggagtgccag cgctgccgga
     9541 taatcgatgg caacaggaag ctgagcagca tcccgggcat ggtggatcgg tgccggcagg
     9601 tgctgcatat cagtgttcac tccacgggga aacgtcgggc aggttttcca caacacggta
     9661 ctttcccgga taacggagct ggttacctcc acgcgtccag acggtgacaa caaaaccccc
     9721 tccctggttg ttttcgggga gcaggcgatc gcactgctca ctcagcatgt ggatcacaaa
     9781 cggcgcatcg ctgccgtctt caaataccag ttccagcgca tcgcggccat gcagtgctcc
     9841 cttactgatg atgacgtact cagccccccg catctccctg agtaaatgtt ttgccgcatc
     9901 cgggacaagc agcctggctg cgccggcatt ccagctgagg tagacatatc cggcctgcgc
     9961 ctgtacagac tgccagtaat ccgttccggc gacagcctga ccttcattct caataaaaaa
    10021 catgggtgac tccttttttc ttttccgggc agggacggtc atgcgataac gtcatccatc
    10081 atcactgccc ttaccgtcac gccgtctgat aacatcatgc cgttatcatc atcaccatga
    10141 taacaagcat ccatataacc acgccgttaa attaccggag agccgtttcc gtcagatccg
    10201 actttccgga ataccacaac tttccggaag tgtaccacgc tgagaaaatc atcatatcgc
    10261 tgttttccgc ccctgcgcgg cgcatcacag gtcagggaga tgacacaaca tatagtgtca
    10321 tcagcagctg aattcagcac tacatgttgt gctgacggga gatggcgctg tgcggaggga
    10381 gaggaagatc cgtaacaatg agttttcgga tctcagtgga aacagacaaa ataaaaccgc
    10441 attcttactg gacgcgtacg ggtttaaccc gtacatttac agtttgtgaa cgtacgcgaa
    10501 tgtaataaaa ccagataaac gaaaccaagt gaggcccccg tggacatgaa agaccaacgt
    10561 attgaactcc gtcttcctca gcagcaactt gatgagctgg ataatttcat taacaacatt
    10621 gacggtcagt ataagccgtc ccgctcggat gttctgcgtt cgtttatagc gcagggtgtt
    10681 cgcgggaaat ttactccggc cagccaggaa gcggagatgt ttcccctgtc agcgcgcctg
    10741 aacatctttt tccagctctg ccagttgctg cgtatggagt gcggtaaaga cggacgttct
    10801 gtccagccta tcaaccccac ttatggctat aacaatcgtg tcgccagtac tgtcacagca
    10861 gaagctctgg tgcgtcaggt ttatctgcaa cgcatgacat ggttctttga gctcgatgcc
    10921 gtgcacctgc aagccattaa ccctaacctg ggccaggaca tgattgtatc cctgatgaat
    10981 ccgcagccca gtccggtgat ctgtaatacc ctggacagcg tgatagcgct tcgtgacatg
    11041 ttctcgaata tcaggatggt acttgcctcg gccgagaaaa cagtgaacga ttggaatgac
    11101 cagaagaccc gcgacgcgct ggcccgcatt cagggctatg tagaagacaa cggactgcag
    11161 ttaactttca aggggtatcc tgacacagaa gactatgcgc tgcagattga catgtggtcg
    11221 ctgcttaact ggattgataa cggacaaggt gatcaccgca tcggcgatta cggtctgcgt
    11281 aatgacaagg atctgacaga taagtacgcc gtcatgcttg aggtgtacca gaatatccgc
    11341 agtaaccatc agttcgatct taacggactc gaacagatgg tgaagagccg tcagttccac
    11401 atgatctgaa aatgagagcg gcttgattct gcatcagaac ggaaaatcgt catgaccaga
    11461 ggcggtgtcc cataactgag ttatagtacc ggctggttct gaagtccgcg gagtatagtg
    11521 gtatgtgctc cgcctgacgg catcagtgga gtattcaaac cgggctgccc gtataccgtg
    11581 aacgctgaca gaatcgagat ctatctccag agcaggctcc cgtgggtcgt cgggaagtga
    11641 aaggtcagac agatcatgcc tggctggagg agggtcttcc tgagcagaga taacctctgt
    11701 cacacagata tcagtctccc attcgtcttc ggcatactga tggccatatc tgatcatgca
    11761 gtcatccttt gccggtagcg tcataaaggc gacacgggag gctttatgga acaacacaac
    11821 gttgtaccgt tcctcctgat ccgcccccgg caactgcacg gacggataca ggataccgtc
    11881 aagattaaag cggagaggat ctgccaggta ttcagcgatg acctgagttg gtatatagct
    11941 gaaatcttct tcgccaggca gaaccggctc gacaatccgg ctggtaaatg tggacaggaa
    12001 tgcggcctgc tcacgtcttt ttatgtagtc cggatcaagc agacttccgc tctccagaac
    12061 actttcaagc gccggcaggt tcaggagacg gagcgaccgg ataagactga acttcgcgca
    12121 caccactttg cagcctaccg gtggacgtat ctcagatata gccgtatcga ctgaactggc
    12181 accatagaat accgaaatgc cctttgcact catacggtta gagccgctga actgatgcgg
    12241 cggcggacct aattctttgt ccggcgtcac aagtatttgc tccagctcat gatgattccg
    12301 gcaccaccgg gcacggtaca gtgacgggat agctgtacct ggtccagcgt caatgatcac
    12361 aggttctttt ccacgggcgc gcattgtctc taccccctgg aatataccgt ccagggtgga
    12421 gctcacggca ttattgatga agcgtgattc tgttttcagg gaggtaacaa aatttatcca
    12481 catggaatcg agacggccgg tactgatctg tttgcgttct gcgtaacagg actcgcgggc
    12541 aaactcggtc tcaagtccca tcacatcgga atcaaaatct gaatgtctgt attcgagcag
    12601 ctgctgaata tcgtctgcga gtgcacgacg agactggagc aaatcttcaa tgagctgcac
    12661 tacattctcc ccctcacgga accactcata accgtcaata tgacgaagac tggcatactc
    12721 catatcgttc ggttcagtgt cagtccggta aaaatgagct tcaatagcct tttctgtcag
    12781 gtcgcatacc tcctccaggg aaaaacaggt gtgaatctca tcgcagtaat cacaggtttg
    12841 tatacgtcct tctttctcga ccttgtggcg taaaaattct tcgccaatac aacaagcaca
    12901 aacccagcgc tgctctgact cttccacatt cataaaaggc tttctccgag ataatactct
    12961 actcaggaag tgtaccagac gtaacctgac ttatatttcc gaattcgccg gaaaccggat
    13021 ctgcgcggca gaaaagacgc aaataatatg ataataaaaa aacacacaca aaatcatatc
    13081 agatatgatt ttcatcattg atgaaaaaac catatctgat attatcagaa atcaggagca
    13141 cacaaatcat gcaggatatg atttttacct gtcagaacat tatcagatgg agcgtgcagg
    13201 atggatgaag aaagggtgtt ttcactcagc tatgagcagt tgacccgaat agcggaaaaa
    13261 aatatccgtg aatgtgggct ggacagccag agcgccaggt gcctcaatga actgagggcc
    13321 tcagctctgc tgtggttgtg gtatgaactg gccattcatg gtgcgccaca gaacaatcac
    13381 gcacaggccc gggaacgcat cgataccgat tatcagcgcc tcaaaaaact catctggtcg
    13441 gagggtgaca gttaatgact cagagccgcc gaccgtcgcc actgcagcgg agagtgctga
    13501 ttgtgctggc tgcccttgat gaaaaacggc cgggacccgt gctgacgcgg gacatagaac
    13561 gggtgctgga gcgaagcggg gaagccccgg tctacgggcc aaacctgcgc gcctcctgcc
    13621 gtcgtctgga ggatgccggc tggctgcgta ccctgcgcgc tccgaacctg cagctggcag
    13681 tggagctgac ggacgccggc cgggccgtgg cacagcctct gctgctggca gaacaggacc
    13741 gcctccgggc agaacagcgt gcggcagaag tcgtcgtact gccgctggtc ccggcagcag
    13801 gtctgcccgc agacggcact tctgccactg atctggcggt ggagctgaac ggcataacgt
    13861 atcaggcctg ccgtggggat tttgtggtgc gtctcgacgg cagcacctgt ctgcagttat
    13921 ggaataaaga aggcagggtg gtacgcctgg agggcgatcc gctggaagtg gcgcagtggc
    13981 tgcaggcctg tcatgacgca ggaatagagg ttcgcgtaca gataaatgaa agtagcgtcc
    14041 cgtaaagggg gcctgagtaa agcggcgcgg atggctgatt atttccaaga ataatcgtca
    14101 gttcaggtat cgtggatacc tgtaacagcc tcctgtgtac gagctaaatc cacacatctt
    14161 ctgccgtagc caggaaccta ctggctgagt atttctggtg gcagagctac gtaagtagcc
    14221 gtacctgtat agtaatcaga tatccagaga aaacgtaatg aaagatacaa ttactccact
    14281 aaattacact aaggaagcca taaaagacct gtctgtcatg gaaaaaggat taaggtcaaa
    14341 tgcacctctt acatcatata ttatgcgtca ggtcattgat atgctggaca gtagtgttaa
    14401 gttcatctta ccgaactgct gcgatattat tgaaccagaa gaataccgcc agacacattt
    14461 cgatttggca cgattaccat atccagttgt aacatttgaa ataccatggt tcaaagacaa
    14521 tgtagaaacc caaataggtg attttaatat atcaccatca tccagaagaa ttgccctgtg
    14581 ctgggaggcc agacagagtt ttgaacccat cccgggatgt aattcaatcc ttaacactta
    14641 tattgatggc ggagtgttta tccttcctgt ttcatggtct gatgatttaa aaatatggat
    14701 tcttggtgtc ggtggaatgt ttttcccata taacaacaaa ctcactaaat acgaacctga
    14761 caggacattg cctgcatcca ggcttgtaat cgacacatta aaagagaatg gagtagccaa
    14821 atataatgca gcacacttca aagcagagcc ttttataaca agcatggaat ttaaagatga
    14881 tttgataaag caggtgggaa gtattgaaag attatatgcg caaattatta tggatactcg
    14941 cgatgaacta caggctttca tacaggcatg tagcgtgtta aattgcgaaa atgtgtgtcc
    15001 tgtcacactt agcactaaac cagaaagaaa attcataaat ggtcgtaaag ttcagccacc
    15061 tgaaaaaaac aagcgtcctt catatactta taaagtattg caactgtctg aaactaaggt
    15121 gcaaagtaat catactggaa caggaaaatc cggtgggacg aaaaggatgc acttacgcag
    15181 aggacatatt cgcaggaaaa ataacaaact aatatggatc agaccagcaa tgattaatgc
    15241 aaacagtaga gcaggcattg ttgataagga ttaccagata aatataagaa aagaagaaaa
    15301 caaaccttag taaatcttac tgtgtaagta acaatccgtt gtatcctgct ataaacgttc
    15361 ctgattcaca tagcagggta cagagtcatc ttcgtaaatt atcaagggat attacataac
    15421 agaaagttta ttaaattttg aactatgata ttataaggaa gtagaacaac atagccgagg
    15481 tatttatggg aaaatatcag gaagaaagga aaactccggt cattgtggtg aaaaaacgtc
    15541 gtactttttc gccaccgtcc ttatccgaaa aaacagatat tatcgcacct gtatttactg
    15601 aacagacggc agaatcagcc cccgccggaa taaattcatc tgccgtcgaa acacatatcc
    15661 ccgaagcgcc agccaggaag aagaaaaaga aaagacaccg ttttccacgt ccctcccact
    15721 ggacccgtga gtatactcac gagtgcgtgg aaaaaataaa ggccctgttt cctcatcttc
    15781 gtgctgaagg tggtggattt attcccctga aaattggcat aaacaatgat atttcggcat
    15841 ttctggcaga acacccggag acagaactga ccatggacga atggctctgt gcagtgtcct
    15901 gcatcacatc ccaccgcgta tatctgcagc ggacagctgt cgccggcgtc ccacgttatg
    15961 gcctggatgg tcaccccaaa gggcaggtct cagactctga agcacagagt gccggtcgca
    16021 gactggcgac attagaacaa aagtggctca ggatgcaggc acagcaggag aatatttctg
    16081 ggcaataaac actcctgata acaggtggac attacgtatc caaagattct cctgatgcga
    16141 aacaggagct tcttgagtgt cttttgtccg gaaaaagaat gaccgggcgg gatgaaatgg
    16201 atatcaagcg cattctaaaa acaaaggaag cacttgataa catcaggaat tttattgata
    16261 aacagcagtt aacgaatagt gatactccga agaaaagcac ggtcccaaat gaagataaaa
    16321 aaggcataag atgatgatca caggaaatgg aataaacact gtaactgtca atggaaaagt
    16381 gaaacacatc acagaactgg attatataac actttgttta gagtggacaa aactacgaga
    16441 ggaaaacaat cgtctttatg aaattaataa caaggcaaac agaggatggc gtggtttcat
    16501 cctaagattg ataggggtta acctgccaga taaaaggact gagtttacac aacgtctctt
    16561 attgacgcgc aaaatttcgg gttcggtcat gaaaaagtaa catattctat gctggaacag
    16621 accaattttg cattcgccgt ccaggagttt gaggggaaag tagtcagtgt ttacgctcag
    16681 tcagggaaag tgtgcaatga taaacaacac gaaaggaatc gtagtacatg ggcaccgcga
    16741 ttcaatattc aaggaaaaca caatacagca atgtacttgt actgtttcta ttattctggc
    16801 gatttcaata aaggcacttt ctcgcgctct gtcattttct gaaactcttc atgctgcatt
    16861 tcgatttccc ttctaagatc ataactcgca tacctgttct ttttatgtat atctattttt
    16921 gtgacgagtt caaaccacaa tgttcgcact ccccaggcag cgttatgaaa catttccgca
    16981 acggctatag agtctgtatt aactgcttcg gtcatgtagt cgctaatacg ttcagcggta
    17041 attttcgtta tttcatcgta attcatttta tctcctacaa gattatttaa tggaatgcaa
    17101 aatatctggc ctgataataa tgttccagaa ttaatatctg caggctatgt taagttgtcc
    17161 ccacacacaa tctacctgtt ttctcgtgtg tgccacaggc gcaatacata aatagtctgg
    17221 ccagtaagtt cgtaacggat ttcgtactcg ccagcaaaaa tccgtctgac ttccctgggt
    17281 tcaaactgaa ataactgttc ccccatacgt ggatgagtta acagaatgac cggagcctgt
    17341 gtcagggact gcaccgttct ggcggccgca ggtttactgg ccagcaccag aaaatcatat
    17401 aaccgcgcca aatcagaaag cgccttactg gtccacttca gttccatcag cgtggcaccg
    17461 gtaacggatt gtcagtactg aggctgtccg cccaggcctg tacagcctga tggtcgataa
    17521 cctgtccgga tgtcacatcg tccagggctt ccagcgtcag gcgattacgc tcctcctcct
    17581 gggcaagcca tgcagaaagc gcctgtttga taatccagcc ccgggagcgt tccagtctgg
    17641 cggccatctg gtcgacttta tcagccatag gtaatggcac atgcgcggta atgacttttg
    17701 ttgcctgtgc ggtattgttt ttcatttgca tatccgatta gctgttaatc atatttaatc
    17761 atagtgatta ctttcagacg aaacaaggca aataatgcgc gtcagcgcat gtaaggcggc
    17821 actccgttgt tacatatggc acagaaacaa aaccgccgaa gcccggcgtc agccggtact
    17881 gattgataga tttcacctta cccatcccca gccctgctaa gccatacccg ttttcagcca
    17941 tgacagagct tctgtgcgcg gtcggagtgg tcccgacgag ggtttacccg aagtcggggc
    18001 gtatctccgc gttagcgggc cgtcagggcc gcttacgagc gtgtactgag gacttccagc
    18061 cagaagactg acagcgatga ggattcttag ttacaacatt cataattaaa agcgatgttg
    18121 ttcccgccct tcgggcgggg gcggcctcgc ttttcagtta tgaggggtag gcacggtgct
    18181 gcgtttgtgt ggtgttttta ttcctgatgt ctttttgttg tggtttcaga acggtgaggt
    18241 tcggggcccg cgcgtcagcg tgggaggtgc atatttgtct gtccacagga caggcactga
    18301 ataggtcttt tttttaaatg gaagtagtta agtagtttaa aggaaataca gtactggggt
    18361 ttaaaagaaa cattgcaccc tgtaacgcct gcggctggcg ctttatagca ttaacggttg
    18421 taaccttacg gagaagtccc ttgcagttaa atgtggataa acagaattcc ccgtctgtga
    18481 gacgtgtttt gcgtgaaaaa cagggggagc aaggcgagcc ggaaggcaga tgatgaggct
    18541 gttttttgtg tgctgtgctg cttttttgtg cgtcgccggg cttcagacgt gcggaagcct
    18601 ccggcgcagg ccggattatt cagatgaggt caaactaagg gtgaggcagt ggccagccgt
    18661 gtgtagttgt tgttccgcga cattatcatg cgttccttca cgcgacggtc gatttcgcgc
    18721 ttcacggcct ccaggcttcc ccggaaacgc ccttccgcga tttcacgtgt cagctgccgt
    18781 ttcaccaggg tgacaatgtc ctgacgggtc ctgcctgcat cgcggcgcgc acgggcacgt
    18841 ttcatgccgt gggacttaag ctctgcctga taactgcgga aacgctcgcg cacaaaacgc
    18901 caggccctcg ctatcagttc atccataccc agcgcatcca tcccctgctt tttgcgcagc
    18961 ctgttttccc attccacgcg gctgcggcgt gcggaagcca ctgcctcttc tgagatatcg
    19021 agtgagtcaa acagtgccgg cgtgaacgtg atgtcggtcg ggatgttaca gccaattgtc
    19081 gggtcgtatt ccgtctggta ggttatcagc cccagctcag acaggaactt cagggcacgg
    19141 gtggcacggg tgatggagag tttcccggcc tcagactccg tcgccagtcc gcactcaatg
    19201 gccagcgtgg tgatggagca ctgcacgcgg ttggccagcg ggtcatagtg gaaacacagc
    19261 ccctgcagca gcgcatcaat cgcccggcgg cgcagcaccg gcggcatacg ccggcgcagg
    19321 ccgcgcgaac gggcatacgc cacatgcgcg gcaaaatcaa aacgggaggt gaagcccacg
    19381 gccttttcca tcagtttttc gcagaacttc agcgttccgg catcttcacg gggtgtgaac
    19441 accggattcg ggttctttac ctgacggtat gtgctttcag taaatgaggt ctggttcagg
    19501 gccgccacaa gctgtcgctt atggcgggaa cagattgccg tgcgttaatg cactgcaaag
    19561 ggttgaatct ctgacacggt ttcatatata cttatcccgt attctgtgag gcccccatta
    19621 tctttctctg attccgccaa gtttcgagga aagatgatgg gggttttcct ttatggtctt
    19681 ctgaaacttt tttcagcctt ccggccccgg caaactgccg tcgtttctgc catacatcct
    19741 gaatgcctta tggcgcattc tgccgttttc tggctttccg gaccgcgagg atcccaaaag
    19801 ccactgcata acgatccgca atatacccct tttccaccgc tttgaaaagc cgacacgatt
    19861 tctcagagtt acagttgccc tctttaaccc cctaaaaagc gtcgtttttt tgaactctgt
    19921 caaaaaaatg aacaatttca caaacgatct tttatgctgc ttttttcttc ttttccagta
    19981 gaagagatcg ccgaattttt ttaaacgatc taatatcccg cacacaacat caaatttaga
    20041 ataatattct gtcttatcaa atataacgat ctattcaact gcttatcctc gctcttgcca
    20101 cagatacatc cttatgtaat atattttcca gccattaatc ctgtttgcag gagttgtcgg
    20161 ttttcgcccg cctcgaaacc gtccacaagg cttacaagag tgcgtaaatc atgtgcctgt
    20221 cagaactgca ggccaggctg aacgtgggtc agccgctgtt tccgacacat gctttatgta
    20281 ttcgccgttc acaacaacat cgaagccccc ctgcggggat cgattttcgc ggagcgttcc
    20341 ggattttgaa tatcagtgaa ttacatcaac gcaactggta tctggcacag tacatccctg
    20401 ccggtaaaaa ccgcgaacat ctcttttcct ggctttccga acaacacgtc cttccctgga
    20461 cacccctgat actgaaaaag gtccgccgca cggataaagt ctgtggttac cgtcgacata
    20521 tacacgccgt gtttcccggt tactttttcc tgaaagcaga ccccgaaagc cacagcttca
    20581 ctcaccttcg ccgtcacagt gcctttctgg acttcgtgaa aatggccggt gaaatcaaaa
    20641 ccgtcaggga ggatatcgtt cagtctttga tgaaagtgta cccggatcct gcgctcaatc
    20701 cggcagccag ggaggagctg gatgctgcct ccaccctctg gctgacaaaa gcgcgttacc
    20761 agtacctcct ccggcttgat gcccagcctc tgccggagtc gaggatcgcc ctgctcctgc
    20821 acctggtatc tgatgacgga gcgttgacat gaaaattgct gtcttaattt gacgactttt
    20881 cccggccttt atggccggga tttttttccc ttctgtttcc acaatgcccg gcaccggtga
    20941 cacctgaggg tgtacggaga gcggctgtcg ggcatttttt caggacgcct ttatgaccat
    21001 tctatcccag gaaaaccctg tcattctgtt actggagaat atgctcgaag cactctccac
    21061 cgccccggac aacataaaca acgaacggcg tcgccggcgt tacctgctga actggcttga
    21121 cactgcccgt cagatgcgcg agttcagagg aatggctgaa gagttcacta ccctgcgaaa
    21181 actgctggcc accaattcag acataccggt agcaaacacc ctgaaatcac tgctggaaag
    21241 tggcaataac gcagcattat acgacctgta ccgcttccgc tcggctcttg ccacgtgcag
    21301 gagtctggga tggcagattg gcacgtgcgc atggaatgac cagttactga gtgagacact
    21361 ttcccgcgca cagaccggga aacgtcatat cctgcagctg aacagcaccg gggacacctt
    21421 ttccaccacg ggcagaatga tcagaccggt ggcattttca ttaatccatc cggccaccat
    21481 caacacgtct gaggttgaag agatattccg ggatgaaggt tttatgctcg cacacgggcg
    21541 cgaatgcagt ctgaacggca gcggcagaaa tatgctgtca cgcattcttc atgtgggcca
    21601 ttcaggttta gcggaagagg aatggggcat ggacaacagt ctgcttttac actgaaatac
    21661 cgggacagcc acaacggcag ccactctccg ctgacttttt ttcgcaaatc gcggtttgct
    21721 gatgtggatg tcccattttt tattatccgt cctctccggt tgtcataaag cgcattccgg
    21781 cgtgaggctt ctgttgttat catcccctcc tgcagacatc cccttgtctt ctgatgactg
    21841 acgatatgct tcttctgaca attaaagaag cggaagcttc acgccataat gttgtttgac
    21901 tgacctgcca caggaggaaa aatgacactg acgttactta ccgcgtataa cgcaccctat
    21961 ctggcagccc tgacttttgt tctgcttact ggtatcgctg aacttatcgc cctgctctgc
    22021 ggacactccc tctcttcagc tatggataca cctgatttac cggagggtct gacaggagag
    22081 gcgctggact ggctgaacat cggacggatc cccctgctga tcgtcctctg tatgctggca
    22141 ggtttttttg gtatatccgg cattctcctg cagggtctta ttattcatct gctgcaggct
    22201 cctgctccga acattctcct tgcaccactt tgcctgctgc tgacatgtcc tctcgtacac
    22261 cgcaccggca gactgattgc cccctggtta ccccgtgatg aaacaacggc gctcaccgag
    22321 gaggcattca tcggctgtat ggccaccatc actggttatc acgcagtggc gggttccccc
    22381 tgtgaaggtc gcttcacaga ccgcttcggg caggttcatt acctgttgct ggagccggag
    22441 gccgggaaag aattcagacg aggagacaaa gtgattattg tctgccgtct ttccggttcc
    22501 cgctggctgg gcgaactgaa tccctggccg gatgttcttt aatcccgaca tatacagagg
    22561 ttctcactat gtttaatctt ccgggatggt tgtttaccgt tatagcgctg gtcctcaccc
    22621 tgttcgttat cgggctgata tttgcccgcc tgtaccgccg ggcatcggca gagcaggcct
    22681 ttgtccgcac aggtctggga ggccaaaaag tggtaatgag tggcggagca attgttatgc
    22741 ccatttttca cgaaacaatt cccgtcaaca tgaacaccct gaagctggaa gtcagtcgtg
    22801 cagcggctga aagtctcata acccgcgacc gcatgcgtgt tgatgttgcc gttgcctttt
    22861 ttcttcgggt aaaaccatca gccgaaggaa tatccactgc agcccagacg ctgggacaac
    22921 gcacgctcac acctgaagat ctgcgtagtc tggtcgagga taaatttgtt gatgccctac
    22981 gggccacggc ggccagaatg agcatgcagg atttacagga tgcccgcgag aactttgttc
    23041 agggtgtgca gaacaccgtt gcggaagact tatccaaaaa cggtctggag cttgaaagtg
    23101 tgtcgctgac aagtttcaac cagactgcac gggtacattt taatcctgac aatgcctttg
    23161 atgctgaagg cctgaccctt ctgacccagg aaacagaacg tcgtcgccgc gaacgcaacg
    23221 aagttgagca ggatgttgaa gtggccatca gggaaaaaaa ccgggatgcc ctctcccgac
    23281 gtctggagat tgaacagcag gaagccttca tgactctgga gcaacaacaa cgggtaaaaa
    23341 cccgtacggc agagcagagt gccagtattg ctgctattga agctgaacga cgtcgtgaag
    23401 cagaatccgc gcgaattctg gctgaacgta agatagagga agccgaaatc gaacgacagc
    23461 agatagtgcg tacccgccag gtcgaagccg aacgggaagt ggccatacgg gaaattgagc
    23521 aacagcaggc aaccgaaatc gccagccagg ccagagccat tgccgtggcg gcaaaatcgg
    23581 aagagcagtc acaggcagaa gcccgggcaa gtaaagcact ggctgaggcc gtacaggccc
    23641 agcaggatgt ggaaaccacc cgccagactg cggaggccga ccgtaccaaa caggttgccc
    23701 tgattgctgc tgctcaggaa gctgaaacga aagcggttga actgacggtt cgcgcacagg
    23761 ctgaaaaaga agcagccgaa ctccaggcag cagcaattat tgaactggcg gaggccaccc
    23821 gtcagaaagg tctggcagaa gcagaagcac agcgcgcact gaacgatgcc attaacgtgc
    23881 tttcggatga gcagaccagc ctccgcttcc gtcttgccct gctggatgca ttacctgccg
    23941 tcatagagaa gtccacagaa ccaatgaaat ccattgacgg tattaaaatc attcaggtgg
    24001 atggtctgaa tggcggtgtt acccgcggta aaacagcaac aaccgacata agtggcggaa
    24061 atctcgctga acaggcagta accgccgctc tggcttaccg tacacaggct ccgctcatcg
    24121 actccctgct gaaagaggtg ggtttatccg gtgaaggact tacagccctg acacgtccac
    24181 tggtcatgcc tgccccggaa aaggttccgg atgtcagcga accggtaacc gctccggata
    24241 actcagaacc acaacaggtt taatccccgt gccggaattt ttccggcatc tttttcacaa
    24301 agaatgccct cacgggatat tctggtttaa aagaggattg aaaatgtccg cagaaaaatt
    24361 cagagttgtc atatacacca atacatgtga aaagaaagtc agttatattc ctgcaacaga
    24421 agatataaac atcgctataa tgccatttca gacggcagac aatggcttaa ttattgtgtg
    24481 tcagaactcc cgtataatac gacgcaaaga gcgtattccc ggaagcagct gtgaatggaa
    24541 agatgtgaca ttagcaccaa aatccagaaa ccgcgttcag ttatagcctg acagcctgct
    24601 tttcatcccc cataaaaggg ggtaaaaatt aaatatttca caaccgcgat atttttcagg
    24661 aatgcagatt attttctgtt atgaccaatt gagaaacagg catgagaaca atcacaacac
    24721 gtgaacaatt attaattaat gggagagtcc acgaaagaat cgccacacat atagttacag
    24781 gagctcatgg gtatgaaacg ctttgcacaa atggctataa cgtcagatac aacaaggcgc
    24841 agcaactggt tgaaagctgc gaaaaaatag ctgaaggtaa actacctgtt acctgtcctg
    24901 tctgtttcac catatggcag gatgtacaca gattcaccca cgttgacttt gacacacaat
    24961 cggggaaaag tgattttatt gatacctgcc attcggagat cataaccgga catgtttcaa
    25021 taacaggcaa gaaataaaat aatgttccag ttctgaaaaa caatatcact tgttttctgg
    25081 aaactatcat ttgcagcagg acgtttttct ttttatattt ccggaaccgg aatgttttag
    25141 ttttcataat caccccgaac atgaatgttc atcatcaata tcctgatgaa attatattta
    25201 tctgaagacc tgttaccagc cacctgtctt ataccgtaac ccgatacata ccggatttat
    25261 tctatgaaaa agaaatcact tacactcctt ctgccgtcat tactcagcgg ctgtacggca
    25321 tttaatgcca cgcctgttcc tgacaccacg cctccggctc tggtgttcgt cgacggacaa
    25381 attcgcgaaa gcgctgccat tatcgcccat acgcaaaaac gtctgcttcc gccaccggtg
    25441 acccgccctg tgacgccgcc ccgcccgaca acaacagcga cgcccacaac cgcagtcagc
    25501 agccagagta ccgcactgcc tccccgtccg ctccttcagg ccacacagct gcccgcactg
    25561 gttatgaccg gtaaacccgg tcagtcatcc gtcgtttatt tacgtgaggc acatcagcgc
    25621 tccgtcagtg actggatacg ccagttgcta ccacccggct ggcagttccg gcaacagaac
    25681 gctgccatac cggcactgaa caaacgcctc cccgtctggt ccgttaatga ccagtggcac
    25741 cgggcactga atgcgctgct ggccggccag cagctttacg gacatatgga ctggaacagg
    25801 aaaatactga ctgtcacgac ctctgcaaca ccgccggtcg acgtcacggc accgcagggt
    25861 tcacagaaag ctgctgatac accgcgtaat ccgttcaggg gcagcgccgc aacgccggca
    25921 ggcccgacac cagtgacacc tgccgtcagc gtttcccggg ctcagaccac caccgggaca
    25981 tccggaactc cagagagcag aacaaccacg ccagccccgg tggccacgaa accggtagtg
    26041 ccaccacagc agaaggtcat accgccacgg cagtggaaaa tggaaaaggg gaccaccctg
    26101 aaagacggac tggcccgctg gacatcatcc gaaaaatgcc cgcacggcac atggacactg
    26161 cgctgggaaa cacctgtcaa ctacagcatc gatgcccctc tggttttcac cggcagtttc
    26221 cgccaggcgc tggagcagct ctttgcactc tataccccgg ctgaaaaacc gctgtatgcc
    26281 cggaccagca ctgcacaatg cctgctccgt gtgacagacc gcccgcagga ataacctatg
    26341 ggatgggtta cgggtttcat tatggtcatt atggccattg gtaatttctg gttccacagt
    26401 cacctcagcc gtactgtgca ccatcagcag accgctgaaa tcacacagca ggctgcagac
    26461 tttattcgtt atatgaatgc catcaacgat tacctgtatc agcaccctga acgacgtgct
    26521 gccggtggtc agctcacgtc agcacagctc ggtctgcccg ccacgaaaaa cgtcagccac
    26581 ctcatcagtc agcagcgcgt ttttgtctgg gcaaaagaaa aacccggcct tatgggtgcc
    26641 ctcctggaac aaagtggtga ttcggccctg cttgccagag tggaaaacgg tcgcctcctg
    26701 gacacccgtg ggcggcgtat ttccatcacg ctccctgctg tgatccccga tcaggtcatc
    26761 atctggatga attaggccgg actctgaata actcacggat ttatctatga acaaaaaact
    26821 tcttgctatt atgattgcag ccactctgct gcagggctgt accagtttcc gacaggcaga
    26881 gaacaccaga aaagacgccg ttgctgaaca acaacgggcc cgggaaatga tcgctgagct
    26941 gcgaaaccgg acaccggttg taaaggaatc cccacgtcag tggatcaatc cccgtccggt
    27001 cacggataaa aaccccggta atgcggtgcc cggctgtccg attatcatta acacccggca
    27061 gagcatcacc cttcagcagg tggcccagcg gatctcggaa aactgtcata ttcctgtaag
    27121 gatcacccct gatgtacagg cgtatctgag cgtgaccacc tccggcagta cgcagcaaat
    27181 ccagggcaca gtaccaccgc cggataccgg cggtatggtt ccactggccg ccataggcag
    27241 ttctgacacg acacttcccc gcctgcaggg aaatacggcg tcacttcctg aactgaaggc
    27301 ttcaggactg acatcactgc tgaatgcggt cgcttcccgt ctcgggattt cctggcgtta
    27361 tgatggttca catatcacgt tctattatct tgatacccgg accttcccgg tgacctatat
    27421 ggacagccag gttgcctata atgccaccgt ggtcagcggt accatgtcat caaatggctc
    27481 atcaggtgga acagcatcgg atgccatttc cggtgatgcc agtaataccc agaccaccac
    27541 cgtggatatg aaatctgcgc tgtatcagga tgtgaaaaat gctgtctcat caatgctgac
    27601 gccgggtatt ggtcgcatgt tcatgtcgac gggctttatc accgtcacgg acaccccaca
    27661 ggtactggaa acggtccgga cgttcattga aaaacgcaat gaagaaatga agcgccaggt
    27721 ggtgctgaat gtggaaattc tgagtatccg gaaaacccgt aatgagcagg caggtatcga
    27781 ctggaacgca gtcttcagcg acagaacgct gggactgagc ctcggcagca cattcaccag
    27841 tgcagccagc gataccgtca ctggtggtgt ttccattgtg aacgggaaac tcaccggttc
    27901 aaaagccttc ctgaaagcac tctcttccca gggagatgtc agtgtggtaa cccggaattc
    27961 cgccgtgacc aaaaacctga ccccggtacc gatgcagatt gccaatcagc agagctacat
    28021 tgagagtgtg acgacggata ccaccgccaa tgtcggctca tccacctccc tgaatgcggc
    28081 cactatcacc accggtttta atatgaccct tctgccgttc attctcccgg actcacagac
    28141 actgcagctg ctctattcca tgagtctctc cgacaaaccg gttattgaga attacgagag
    28201 tggtggaagc aaagcccagc tgccgaatgt ggatctgaaa accatcaacc agacagtgga
    28261 cctgaaatcc gggcagacag tcattatcag tggcttccag cagtcagggc gtcgcagtgg
    28321 aaaacagggt gtcggcacac cgggattctt cggtcttggc ggtgggatca acagtgaaaa
    28381 cgacgacacc attctcgtgg tgctgatcac gcccaatatc atctgatttg cagaaggagg
    28441 actatcgtga cagatatttc accgacagag cgacaggtgt ttccgctgac ggcaggacgg
    28501 aatgtatttc ttgccggaat ggactggaaa acactgcccg caagccataa aaacccccgg
    28561 acgtttgccc gcagtctggg ggcagtacgc tatatttcct gtgagtatct cagtacggaa
    28621 gataccgatc gtcacatcat ggttgcagcc gtcagccaga atacaatccc caaaggcagc
    28681 cgcaggtatt tctctctcgc catgcttatc ctgcctctgc tcgagccagg aggatacgct
    28741 atcgttgaac tgtcacaggc aaacgataca gaactttatg gttttgtttc tgctgttgac
    28801 ggcatcctgg tcagtgacct ggtgggaacc cgggaggaaa tcagggaggc ccgggaaata
    28861 ttcctcacca ttaacagcgc gcctgaacac ggctggacat gttatgaacc gccctcgttt
    28921 aacgggcctg acggcagagg tcccctgcca ctggaaaccc tgacaggtgc agggaaatat
    28981 ccggcagaag cacgccttca ccccgtatcc cgtgggccac agcttattcc ggtattactc
    29041 atactcaccc ttctgggtac tgcatggtat ggctggcagt actatgaacg cctgaaggca
    29101 gaaaaagcag cacttgctga agcggcacaa aaagccgcag aggaacggat cacaccaccg
    29161 tggctgagta tgccggaaac cggagatttt atccggggat gcagtgacgt atggtcacgc
    29221 ctgccggtgt cggtcgccgg ctggcgatat tcactcgccg agtgtgctgc caccgggaac
    29281 agcggcaatc tgcgtgcatc atatacaaac atcgccggga cgacagtgac cgcattcagc
    29341 cagcgggtca gggaaatttt cagcatccgg ccgtttatgg ttatgccgga cgggaatagc
    29401 ggtggttttg ccctgccggt caccttcagt atgcctgaaa ctcccgttgc cgttgaggca
    29461 ttaccggaaa acaccctcct gcaggaacgg ctgaccacac tcgcccggtc gatgcagctg
    29521 aaaatggact ggcaggaagt cagcaacagc ttcaccgatg aagacggaaa taccatccag
    29581 cccccctgga aggagtatga cctgcagata ctgacgacac ttccggcgca ccaggtggca
    29641 gagcatttca gtgaaccctc cgtccgtttt atctccgtaa caaggcaact ggaagagggt
    29701 cgtttccgtt atcaattcac aggtaaatat tatgtccagt aaaacaaaag ctcttctgct
    29761 ttccgtcctg atcctgccga caacggtcgc ggccagcacc atcagcaaca tgcaacagaa
    29821 taaccggcaa ctccaggtgg aacagattca ggaagaaact gcactgtatg aagccatgct
    29881 ggcaagagaa aaggtgcgac atgaactcag aaccctggag cagggagaac cagctgagcc
    29941 tgtgcattca caggaaagca gttcagccgt cacgacagat acccctgccc ccattaccgg
    30001 tattccccgg ctgacagaga tatccggagt tcacccccag ataaccgcca ggatacaact
    30061 gccgggcgga caaactgctg atgtaaaaga agggcaacac attcccggca ccacattccg
    30121 cgtggaaaag atcaccactg acagtgttgt ccttgaacaa aatggtatca gaacaacact
    30181 acaaccctga ctcagtgccg ccatttacag ccggctgtcg gaatagatta ttcccccggc
    30241 ccttaatcac aaaaaaaatc gtttccgggt accgctgccc gtaaggagat tttatgctgg
    30301 cacctgcaga ccagccacct gatgacattc tggattacgt ggttgttgat acagataaaa
    30361 caggacaaat tcatgtcaga gtggtggaag gaaaaaaaca ccttcgtgcc gtccaggatt
    30421 atctgacccg cctgtgctcc cgctatccgg gtaaagtatc cccgtttgag ttcaccacac
    30481 ttgatgtgat cgcccgactc aggcaggaga cggtcatttc cggggtggaa aacggaatta
    30541 accccgtaca gcaaaaaatg ctcgggtata tcagccatgc ggccaccctc agcgcctcag
    30601 atcttcacat cacgccgggg cgtgacagta cggatttcac gtatctggag gcccgtgtcc
    30661 acggagagct ggagctgctc gatatcgtca gaaaagatga ggggctggaa ttactggggg
    30721 ccacctattc cggaatgact gacgtcataa agggaacaca gtttgatccc ggtgtccccc
    30781 aggatgcccg tctggctgaa agattcctga aactggccgg actgtttggt gcgcgctact
    30841 cccactatcc gtgtgtgggc ggactctatg ccgtactgcg tctgatcaag gatgacagtc
    30901 agcatatacc gacattcagt atgctggggt atcaccctga gcaggagcgc gctgtccgaa
    30961 ggatgctgca gcgaccggag ggcatcgtga tcctctctgg ccccaccggt tccggaaaat
    31021 ccaccacgct gaggacggct tctgccgcct atctggaaca gtatggcttc aacgataccg
    31081 gagggatcct gctcccccgg cgacgtctgt tcacaataga atcaccacca gaggggcgta
    31141 tccccggtgc aattcagacg gccgttatgg ataccgctca gggctgggtc gattctgtta
    31201 agtccgccct gcgccttgat cctgacgcta tcctgaacgg ggagatacgt gaccatgcct
    31261 ccgccatcac ggcgataaaa gccgccatga ccggtcacct gatgctgacc acccttcatg
    31321 ctaacgaccc gataaatatt cttgagcgtc tggaaatgga gggcgttcag gcaaggatga
    31381 ttgcagatcc gcagttattt atcggcctgc tcagccagcg tctggttcag gtgatatgtc
    31441 cccactgccg cctcccctgg catgaggtgg agagctcccg gacggatgaa gaacgacgcc
    31501 tggtggaaaa cttctgccag cccgatgccg tgtatcttcg taaccataac ggctgcccac
    31561 actgctggcg tggagtgaac ggccggaccg tcattgcaga agtgatttcc ccggatgcga
    31621 agtttttcca gatttaccgg gaaaaaggcc ggattgaggc aaaaacctac tggcaccggg
    31681 agctcggcgg tatgacacga aatcagcacc tgctgggcaa aatcaacagt ggtcaggtcg
    31741 atccgctggc tgcgcattac atatcccctg tagacgagga cagctatacg ctgctgcact
    31801 gatatgaaaa ttctggaaag actcaacaat acggaactgg aactgtccgg ccttaaccgc
    31861 tggctgggca aaaagacgtt caggcggact acgttttacg agtcccttgc aggcatgatc
    31921 cgggacggca caccggttat gcgggcactg gagttcatct gcgatgtgga aacggatttt
    31981 ggtaaaaaga aagggcagtc cggcctgtat tttctggcga cagactgtat cgccagcata
    32041 cgcagcagtg gtcagctctc tccggccctg aaggactggg tacccaaaga tgaaattgcc
    32101 ctgatccgta acggtgaaga acgaggtgat atcgctgaag ccatgtttca ggtcgtcaaa
    32161 acggcaaaag gacgacagga gatgattagc agtctggtga gtgtctgcct ctatccgctc
    32221 atcctgctga cactctgtgt ggtgaatatg tataacgttc atacccgggt tattccgatg
    32281 atcagcgcct ttgcaccgga agaacgctgg tcagtacaga tggtcattct ggccaaatcc
    32341 tccggactgg ttatcggtta tggtttgtgg ctgctggcag gactgctgct gctggcaatt
    32401 ctcatcagga tgtcattcag tctgtatacc ggtccccgac gacggtatct tgatcgcatc
    32461 ccgccatggt ccctgtatca gactttccat ggcgtgaatt acctttttaa tatttcatcc
    32521 atgctgcaga taaatattcc tttatctcat gcccttacca gaatagaaaa acatgcagaa
    32581 cataaccgat ggttaaaatc acgaataagt gctatcagaa aaaatgtctt atccggtcag
    32641 tcactggcac tggccatgaa aaactgtggt tatgactttc cgtcaaaatc ctgtgtgaat
    32701 aatcttcttt taaattcaga aagggaggac agtgttgcca gtatggcgct ttatgcagac
    32761 cgctggctgg aagaagcaaa aaagaatgtc agaaaaagcg gcattattat tactgctata
    32821 tcaggtctgc tggtatttct cttcatcatc aatatggtca gtgccattta cggcatcagt
    32881 gacatgctga agcagtaaac acagcatcct ttatattccc ctcgatccaa attctgtttt
    32941 accaatctca ggagctaata tgagcgtcat aacctcttcg tattcagaac ataatattcg
    33001 tcaccttcag aacgatgata aaggcatgac cctgctggaa gttctcggtg ttctggtcgt
    33061 cgctgccatt gttattggtg ccgtgatggg actgatgtcc gataccctgt cctcttcaga
    33121 caaccagaag gaactcaaaa acctgcagac aatcgccacc aaaatgaaag cccagaaatt
    33181 ccaggggcag tatacaggta ctgactacgt caaaatcctg accgaaagtg gtggcctgcc
    33241 tgccgatatg atcgccggtg gtaataaggc gaaaaatgcc tggggtggtg cggtaaccat
    33301 caaagtcagt tcagataaat actcctacgt tattgaatcc agcaatgtgc cgaagaaaaa
    33361 ctgtattgat ctggtcacca gcctgcgcag ttcatccatg tttacaaaaa tcaacggcaa
    33421 tgtgacgaat aaagttgacc cgtctacagt atgtaacgca gataaaacta ctatcaaact
    33481 ggaaaccaac agttaatcat tcatcagcgc ccgcccgggc gctttattta aggtcactgt
    33541 atgaacaggt tatttatcct gacagtgatt ttttcactgt tatgctcact tccttccgca
    33601 caggcatccc cacaggacaa acaatatgat cgttgtttta ttaccgccgg aaaacgttat
    33661 tccattgatc caggattact caaaattatt gcccgcaggg aaagcagtct gaatccccgg
    33721 gccattaatc ataacaggaa tgacagagga aaaattatca gtaccgatta cgggataatg
    33781 cagattaact ccacacatat tcccgggctg aaaaaaatgg gcattatccg gagtgagaaa
    33841 gatctgttga ataatgtctg cctgaatatt cagaccggag catggatcct cgcccggcat
    33901 tttcagcgat gcggcgtaaa ctgggaatgc ctcggcagtt ataacgccgg cttcagtaaa
    33961 agtaacactc accgccggat gaaatacgcc cggcagattt acagcgctta catgcaggga
    34021 cgttagtccg ggagaactgc catgctccag ataatcccgg cactgctggt cgccataacc
    34081 gtggtaatac agtgtctggc cgttcagatg agtcgctatc gactctcccg gatgaatgcc
    34141 cgcccccctc accatgggct ggcgctgatg agcgggatgc ttgcagctgc cgccattatc
    34201 ctgcgtcttt atcagaatga cagcgctgtc ggggtgctgt ttatgacgat aatgaccgga
    34261 tgtatgacac tgatgaccct gacggatgct gcatcctgcc gtcttccccg gcccttcacc
    34321 atcatgtatc tgataagcgg cagcgcattt cgtctctggc agggagactg gctgacaggc
    34381 ctgtgcagtg cagggttctg gttcctgctg ttgcttctgt tcaggcaata cacctcttac
    34441 cgcaggaaaa cagaaaccat aggcctgggc gatgtttttc tgattgccgg tattgccatc
    34501 tggtccccgc cgggagacat cccctggatt gtcgcaacgg cagcaggcgg agccttactt
    34561 tatctgtggt gtaacagaca acgacgcatt accagagaac ttccctttgg gccgtttctc
    34621 tgcgcgtcac agtacgcatt tactttttat cccggtggcc tttggtaata ccggagggaa
    34681 cgactatgca aaaagacaac gataaaggtt ttgcacttct tgaaattctg ggaggactgg
    34741 ttgtcatcag cctgctcatg ccgctgttct ggtcttacat tgaagactac ctgaatgaaa
    34801 tgcgcaacca gagcgccgca tttcatgcag atgcctacaa tactgcagcc aggacctaca
    34861 tcgccgataa caatgcccgc ctgcatagcg gaacgcttcc tgccaccttc acggcggatg
    34921 agttaatccg gaaaggatat ctgaagggtc ttaaccgctc gccctttggg cagagttata
    34981 ccaccggcat ccgtcgtaac acgagtaccg gtcgtctgga ggccctgacc tgctccaccg
    35041 gaggggagaa tatcaaagac gatgccctgc gcagtatcgc cagcctgctc cccggtctgg
    35101 gcggatttat aggaaaaaat ggcacagcaa ccggtgtttt tggtggatgg acagataaac
    35161 ccggcgacta tggactcagc tgcaatggcg ggcacatcgc catcgtaatg atgggtgatg
    35221 acctgcagga aagcgatcgc ctgtatcgct ttcaggtacc gggacgaccg gagctgaacc
    35281 agatgaacac ggccatcaat atgggaggga acaacctgaa caatgcagga aacgtcaatg
    35341 gacaaagtgc aacactgaaa ggtgatgtta caagcgaaaa cggctggtta atcaccaaaa
    35401 atgataaagg ctggaagaac atcacctatg gtggcggttt tacgatgaca gacagccagt
    35461 ggatacgcgc agtcggtggt aaaggaatta taaccacagg ggaaatcaaa ggaggaaaag
    35521 tcagtggggg gacagtccgg tctgacggtc gcctttccac aggtgagtat ttacagctgg
    35581 ataaaacagc cgtagcaaat acaaagtgtt caccagatgg acttgtaggc cgcgacagca
    35641 aaggggcaat actttcgtgc caatccggtg tgtgggttgg ctttgaatcc taccctgtgg
    35701 gaagccctat cccgtggcca tctgccactc ctccacaagg ctatctgttg atgaacggcc
    35761 agtcgttttc ctgttcccgg tatcctcagc ttgcccgtgc ttatcctagc tgtaagttac
    35821 ccgatctacg cggtgttttt atccgtggct gggataacgg gaagggactt gatggtgatc
    35881 ggaacagaca gctcctgagt tatcaggccg atcaatcagg cgtctatcac gaacgtggcg
    35941 gatggctgaa agggcatcat agtggtatgc catactgggc acaggggagt accacagaaa
    36001 tgcgtcccaa aaacattgca ttcaactaca ttgttaaagc cagctaggga ctcacatctg
    36061 ataggcatac acagagaaaa caccgttacc acaggcgtaa ttttgtgttg gtctggagat
    36121 gatttgatat gacgctccca ctggcacggc aaatgaaata aatctccggg catatcagct
    36181 ggtcagcaaa ctcgatgcca ggcgtaaaca gaccagcaaa atttcccctt attttgttcc
    36241 ctaccctgca actgtcgggt gcagaaacgg gctgtttgtc gtgacactgc acgatttcga
    36301 ccttgaaacc cgtgcagaaa cccggggact ggcaatatct catgccagca tgctcagtca
    36361 cctcctgtcc gaatcctttt cattcgccct taaaacagag ttgcaaacac tcttgaaaca
    36421 cactcgatca taacgatcga aataactcgc ttccgtttaa aaaacactct ctttagactc
    36481 aatttatgaa caaaacacgc tgacccgcat ggaggtgaaa tgaaaactga cagcacaggt
    36541 attgccgccc gtatgatgct ctccctggac agggagcgaa tttgcgaatg cctgctcagt
    36601 catcgccagc tgcagtccac acccctgcag gttcgttatc cgcagggagt cagggatgcc
    36661 cttggtatca tgagcgaaca gctttctctt tccgtctctg acctgacgcg tatcctggtc
    36721 gaagacgcgt taagtgaaat gtttcttcct gcagataaca tcgtccgccg tcttctcagc
    36781 aggatggagc acatcatgca ggcacatgat atcagcgcaa caacgatggc tgcattactg
    36841 gccccctgga atatccgccc tgccgtcttc cgggagccgg acagactcac tgactatctg
    36901 accggggaga ttcttgcagc actggctgac tggttttatc tcagtccgga atggctgaac
    36961 ggcagggtgc attatccact gtatcgtccg ggagactggc cagccacaca ggagatattc
    37021 tgcaggatta tttcagcccg cgagaatatg gacattattc tctggcatgg ttttccgttt
    37081 gccggaacgc attccggaga atattgcggc gttctgctca ggcagaaaaa agaaattaat
    37141 aacaccatta tttatccggt actttcgtta tatccggcca gaatggatat agaaaaagaa
    37201 ggctggtttc agatggcacg aaaaatatct ccggatatcc ctgttcgcgc cgtaacactc
    37261 actccggcac aggcagaata tctgataaca ggaaaaatat tacccactgc gttattcagg
    37321 gttccgttat ccccctggta ataatactga gcaataaacc ggaataatac accaactgtt
    37381 tttaatggca cagccgtgcc ctctgttgtt atacccaaaa tacctcaata caggaaactg
    37441 aaatgatgat gaaaaagcat attacccgta ctctcatcgc gtctgccgtt ttattttcct
    37501 ttaattcgac tgcagcaacg tcatattttg aagcccgcaa tgatgcgatg ggtggaacgg
    37561 gggttgcctc ctctcactat ggtgtggcac cactggcaaa cccggcactg ctgacaaaat
    37621 ccggtgcaaa agatgacttc agtcttctgc ttccgtccgt gggtgcgcag ctgtccgatc
    37681 cgggtaatat cgccgacaac gccgacaaaa tcagcgatga ctggaaagcc ttcgaccgtg
    37741 ccgtcgacag ccactccggg gtaccgcaga cagcggcccg cctgaaagag agactgcagg
    37801 acttccggaa cacccatgcc agcgcacagg ccggggtatc tgctgtggcg gcccttcccg
    37861 gtgataccct cgctgctgca ctgatgctga aaacgctcgg gactgtcagc gtcgacggta
    37921 aagtcagcga tgccgacctg aactatctgg aaagcattgc agacagtggc agccaggatg
    37981 tggataaaaa cagactgacc tcacaggctt ttgcccgtgc cgcactgatc accgatgtgg
    38041 gcgttgcgct ggccactgaa ctggaaacgg ccggacaaaa atggtcactg ggcttcacac
    38101 caaaattcca gcgtgttgac ctgtttaact acaatacgct gatcaaaaac tatgacagca
    38161 gcgcattcaa aggtgaccgt taccacaaca cccaaaacgg tattaacgct gatatcggtg
    38221 ccagcatgga cctggatgac aactggacgc tgggtctggt cgcacagaac ctgatcccac
    38281 gcagcattga gaccaaagag gtacatggtg tcactgaaac cttcaggatc cgtccgcagg
    38341 taacaaccgg cgtatcatgg cacaacgaca tgttcaccac ggcactggat gtggatctga
    38401 ccccggccag cggcttcaca tcagacagca aacgtcagtt tgccgccgtt ggtgctgagt
    38461 ttaatgcctg gaaatgggca cagttacgta ccggttaccg ccagaatctg gccagcaaca
    38521 acggcagtgc attcactgcc gggattgggc tctcaccgtt tgatgtgatt cacattgatg
    38581 tcgccggcct cgtgggcacc gacaacaact acggtgccat tgctcagtta cagttcacct
    38641 tctgattatc acgccccggg acgcccgggg cttctgacct gaatctgctc gtttccgctc
    38701 ttctggccac tcaccgccag tcctgttcat tattttccgg ataaaaccat gcaaaaaaaa
    38761 cgtatcaccc ttgcaggggg agttgccatg ctctgtctga gcggctgtca tcttcccccg
    38821 gataataccg caccggccag gaagtacggt gcagcttctg aagtcgctgt cacacgacat
    38881 gcacagtatc agttgtggga gcagggcacg tattctgcag ccctgccttc ccgccagccc
    38941 ggaggcagcc tgcaggctca cagcgggcgg gtttcctttg actgggaagg cgatgctctg
    39001 gagctcctga gcgagctggc ccgtctgcga ggacaacagt tcagttacag cggcgtgcgt
    39061 cttcccctgc ccgtctcact ccatgtacgc gatatgacct tcgctgatgc ccttcgtctc
    39121 acagaggccc agactgcctg gcgggcagtc ctctcacagt cagccgggcg tttacatctt
    39181 tcctatatgc caacggagaa aaaatgaaaa aacaggtctg tctcctgctg tccgggctcc
    39241 tgctggcagg ctgtgccgga cagcaccatc gcccccctgc cggtatggca ggcaatttac
    39301 caccgccgga tctccgggca tggctgacgc ccactgacaa ccaggatgac ggcatttcag
    39361 acacccgtcg ccagatgctc acggacgccg gacgaacgct gggattccgt ggcggaaaag
    39421 cacagcgctc atgggaactg attcaggcac tgaatgcccg ggaaagtaca ctgaatgccc
    39481 tgtatgactt ccgtcccctt atcagcaggg aaggctggct accccctgtt attgatgaag
    39541 cgcaggatgt tgctcacatt cagcctgacc agataagaac cgcgtcccgt gtctggacca
    39601 tcctgcgccc cgaacgtttt gtcagtaacc cgccgggctg gcgtgactgg cttctgcggg
    39661 ggctttccac taccgccacc ccgggaactg agggccgtgt tgtaccggaa gaccgcgcac
    39721 agcgcagact ctgggaaaac gcactccgcc agggctggca agaaggccgt gacaacgctg
    39781 acctcaccct ggaagccaac cagaaacgcc tgacccggga ttaccgggga atgatgcttt
    39841 atgccctgct ctggcgtcag ggcatgatca cacgcccgga tgtgactgaa cagcggcaga
    39901 ccgtcaccgg taacggcagg aaactcatca ccggagacca cgtccgccgt ctgaagacac
    39961 acgcggaatt caccctgcag aaatcacgct ggcgtccggt cgtcagcacg gaaggagccc
    40021 ccgatgaaat tacccgatga gttcggcaca ttccctttcg cccgtttcac ggccgaagag
    40081 tttcgctatt tttttgcctg gtgtgcccga caccgcgtca gtgatatcga tctgacaggc
    40141 ggctcaccgg tatctgtcag tcgctttgga cggcgggtac gttcgtccgc acatcctctg
    40201 ccgtcctcca tcatgatggc gctgagtgat gaattgtttg gacgggaagt ccttccccgg
    40261 gtgctgtccg gtcatcccgt ggaccgtacc attcaggtca gcggtgatgc ctccggtcgc
    40321 tacggtctgc aacgggggca gcgtgttcgc ctgcgctgtc atctgattca gggaacatca
    40381 gcaacagaag aaaaagtaat ctccgtcacc atgcgggtga tcccgtccga tattccggac
    40441 atcctcacca tgaatattga accggacctg ctggatgcca tgctccggaa aaccggtctg
    40501 ggtttcatct gcggagagac cggctccggt aaatccacac tggcagcggc actgtaccgc
    40561 tacattcaga cgcatttccc cgaccgcaaa acggtgacct atgaagatcc ggtggaatac
    40621 attctggggg gagagaatga ccttctgcca ccccatcagg ccgaaatcgg gcgtgatgtg
    40681 gaaagttttg cggcaggact tcgctctgcc gttcgccgta acccggacat catcggtatc
    40741 ggtgaaatca gggattatga aacggcagat gcagccgtcc aggcaggtaa caccggtcac
    40801 ttctgcatcg gcaccatgca caccaaatca cccggtgaaa cctttgcccg gctgctgggg
    40861 ttatttccac cggaaatcag ggattcaatg gcagccgcca ccctttccct ggtgcagttc
    40921 attctggtgc aggtcctggt cagaacaaat gacggtggca gacaggccgt ccgggaatac
    40981 atcgtcataa ctgacgaact gcgtgatacg ctctcccggc agtcccatgc gacatggggg
    41041 cattacattg atgaaatcat tcgcaaggaa aaacggcgga tacgggatca ggttctgacc
    41101 atgtatcaga cgggcagcat tgaggcatca gaagctgcgc tcttcatccc ggccggagaa
    41161 ttccccaaat gaaacgcgaa ggcttctggc gggatgtcac tccgccactg aatatttatg
    41221 gtgtcccgtt gccgtttacc atgatttacc tgctgctgtt tccgtatccg tcaaaaacaa
    41281 cattctgggt gtgcagcggc attttgctgt tttttgtgct cctggggtac tcaggatgga
    41341 ctgtccggac gctctgccgg cgtctgtaca gcctggtgca gggtaaacag gcgtcaggcc
    41401 gtccctggtg gtaccggcat cgcacagaat taccccgtga atggcgcgga acggaataac
    41461 cctcttttgt tcatcatccc ggcgtatcac aacgccctca tctttaatcc ccggagaacg
    41521 acatgaagtc cagatatcga acctggcttg ctgtcccgcc tgaggagaca gaagccgtaa
    41581 aaaatgcagt tcccccgtta aatggccgca aagctgtcgc atgggatcca gaaaaaaagc
    41641 tctggtatgc ccgggcgggt accgaacttt cactgctgga gcgctggtta ccccggccac
    41701 aggaactgtc gatggatgcc ggcgatccgg tcactgaatt tgctcaggtc ctggaaaacg
    41761 ccgggctggt tattcaggga ttgccacaga tggacggagc catacacaga gtggccaccc
    41821 gggatgacaa aaaaggggcc aaaagtggcg catacagggc ctatcttgat ggtcgtcccg
    41881 ccggctggta ccgcgactac cggagcgccg atgacagtcc cacaaactgg gtattttccg
    41941 gcggagaaca acacgatcct ctggccagac tgcatctgcg ggcattcgct cagcaacagc
    42001 gcgatgataa cgctcgtaaa ctgcagcaac agtataacaa acaggccggc tatgcccgct
    42061 cctatattaa caggttgcca caggcaacag cacatgaata cctgacgcgc aagggcatcc
    42121 gggccgcgcc tggcgtgcgg ctgaacaata aaaatgagct ggttattccc ttcagcaacg
    42181 gccggggaga aatacgttcg taccagcgta ttccggtcac cggcgggaag gatgcccgca
    42241 ttctgaaaga ctccgagaaa accggcaact ggttcacctt cggaaccccg gaaaatggcc
    42301 gccccctgtt atttgcggaa ggttacgcta ctgccgcctc cctgcacgaa gcaaccggcc
    42361 ttccggttct gatgacagtt gatgccagca atatgattgc cgtggcagaa aatgcccgcc
    42421 aaatctggac tgacagcccc tttgtcttct gtgccgacaa cgatcatcaa cgggaaatta
    42481 acaaaggggt ttttagtgca acaaaggccg cagaagtcac gaatggcgaa gtcattattc
    42541 cggcctttac ggaggcggaa aaagcacagg gactgaccga tttcaatgac ctggacgcca
    42601 gccggggacg ggacaacttt cagaatgcca tgaatgcaca gttaaaacat atcggtatcc
    42661 ccacacccaa cagtgacact gccgaccatc gtgaggctgt cgtgatcgac aacctgatat
    42721 tcacaccggt aaaagatgag aagccacaaa tgtcccctga aaacagacag agcacagccc
    42781 cggaaacgga gctggcgaca caggatacgc cttatgacac ccactccgac gatacagaaa
    42841 accaggcata tgaagatgac tatgccgcct atgcatccct gcaatcagta actgcggaga
    42901 cgttatcccc ggataaacca ctggagaata ttgcgtctgc cgctgatacc ggcacagaga
    42961 atacctctgt gcctaccgct ggcgaccagg aagacctcaa tacagacgcc acaggtacag
    43021 acaccgctca caacagcgac acagcagaaa caggaacgct tcctgaacct gccgggatgc
    43081 agtccgtgca gcaggcaacc actgaaccga ccgcagaaga taaaccacac gagaacgttg
    43141 tttccgctgc cgataccaga gaagaaaatg cctctgtggc taccgctgat accggtgaag
    43201 aaaatgcctc tgccaccacc gctgacaacc agagcacgca ggcagaagaa catccccgga
    43261 gtaaagacac accggaaacc tccctctccg acgcggccgg gacccagcac accaccacaa
    43321 cgcagtctga agaacaagtg caggtttcca tggccagcgc tgacgactgg agcgcatttg
    43381 cggaagatct caacgctgac actccggggg cccccactgt tcacaacacc gacaacgcgg
    43441 agacaaacac agtttctgaa ccggccggga tgcggccagt tcagcaggtg accactgaac
    43501 tgacggcaga agataaagca caggaaaaga gcgttgcgtc tgccgctgag accggcgcag
    43561 agcactcctc tgacatcacc gatgacaaca ggaacgcacc cgctgaggag ctcaatgccg
    43621 atgccacggt tgctgttcac aacaccggca caacagaaac agcctcacct tctgaacctg
    43681 cagaaactca gccagtccgg caggccaccg ctgaaccgac ggctgaagat aaactgcagg
    43741 aagagcgtct tgtttccgcc actgagacca gcacagcgaa tgaagcagcc cgccctgaaa
    43801 cgccatcgct tttcagccgt ctgcgccgtt ttctgaccgg tgtgaaatca cgcacctcag
    43861 aaccttccgt tattcaggag aaaaagccca cggaggctga aacagcaaca gaggaggtcg
    43921 caccaccatc accaccggaa accagcccgg aagacacatc gtcagcggaa ccggacacga
    43981 ttatctttgc accccgccgt cccgacagtc cgcagcggca caacctggac gagatcctta
    44041 aaaatcttac acacaaagag tttcccgaca ggactgcgct ctacagcgtt gacggtgaac
    44101 ccgccttcct ggacagactc tactgtctcg aaatggtgga tggcgccagc gcggatgaca
    44161 aaaaggtcct ggctgccctt gctgtggcca ccaacttcta tggcggcgtt atcgagctga
    44221 ccggcagtga tgcctttaag caaaaagcca tgcagctgat tgtcgaatac gacatcaagg
    44281 ttcgcatgaa actcccggcc cagcgggcag cactggaaaa aatccgcaag gaaaaagaga
    44341 tggcgcatga tgccattgtc acccacagac ccacgccgga actcaaccgc cagtcaccgg
    44401 aagagacaac aatggcccaa ccggctcccc ctgtgtcacc gaaggcagag gctgaacaaa
    44461 cagcaggcag tacggctgaa acaccgcatc cggaagcgcc ggtcacggca aaagctgaag
    44521 ccccccgggc cgacagaccg gaacaacccg ccgccccgtc gtctgcgcca gaaaaagccc
    44581 ctgtcagtgc acagccgcag caacccgaca caccggagga agagccgccc ggcaagctca
    44641 gccccggtga aagtgtgact gccgtcctga caaactacgg agaaaaagag tatgcgcccg
    44701 gaaaagggcg ctgctttttt gtggaactca ccaaccgcag tggcaaacgg gaatactggg
    44761 gaaacggcct ggaagaactg gtcaaaaacc atcagaaggg tgaccccgtc acactgacac
    44821 tgcaggcgag agaaacattc cggctgtccg gtgaaacgcg ggatcgtatc cgtaatatct
    44881 gggccataac cccggtcagt aacggcatta ccgttaataa tgaccaacct catgcgggtc
    44941 agcataccct ggagtttccg atggcaacat tcagccaggt gatgttacag atgcaacaac
    45001 actggccaca gataatgcag gaagttcgct cccccaaaaa tcctcccaat aatctgtaca
    45061 ttggtgaaga caggcaacct gcatcagcac ctcaggatca atcaatgcga ctccccctcg
    45121 ctggtaatgc ccccaatgaa ctgataccag ccatcgcctc tgccgataag gacaatcgtc
    45181 agttgaatct tctgctggtc cacagcgcag atgatcatct gcagggcgtg gtccgcctga
    45241 acggcaccct ttacccggca ctggccaccc cgtcagctga caaccgtcag ctggttatca
    45301 atgccctgac ggataacggt ctgcagtttg ccggttacgg tgaagctgtt aaccatgatg
    45361 agaataatca tcagcgtccg tccccacaaa taatgcagtt tcacctgaag caacaggact
    45421 ccccgttgtt tgccgccatc cacaaaccgg aagagcaacc ggacaagctt ttccgctctc
    45481 tgggttttga gcagacctgg aaagagtggt ctgacagcca aaaggcggag gacaggcagg
    45541 aaaaaactct gcaacaggca caatcccaca gccccggccg atgagccggt aaggacgacc
    45601 atgaacatga aaaaaacgct gctggtcata ctgaccggca tcaccctggc atcaccggtg
    45661 gcaggtgcc
//
LOCUS       DAJTVE010000015        43207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000015 DAJTVE010000000
VERSION     DAJTVE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..239)
                     /locus_tag="OO439_000827"
     CDS             complement(<1..239)
                     /locus_tag="OO439_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6315272.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCL"
     gene            complement(442..678)
                     /gene="feoC"
                     /locus_tag="OO439_000828"
     CDS             complement(442..678)
                     /gene="feoC"
                     /locus_tag="OO439_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6315273.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(678..2999)
                     /gene="feoB"
                     /locus_tag="OO439_000829"
     CDS             complement(678..2999)
                     /gene="feoB"
                     /locus_tag="OO439_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6315274.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3016..3243)
                     /gene="feoA"
                     /locus_tag="OO439_000830"
     CDS             complement(3016..3243)
                     /gene="feoA"
                     /locus_tag="OO439_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6315275.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(3681..6002)
                     /locus_tag="OO439_000831"
     CDS             complement(3681..6002)
                     /locus_tag="OO439_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6315276.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6099..6575)
                     /gene="greB"
                     /locus_tag="OO439_000832"
     CDS             complement(6099..6575)
                     /gene="greB"
                     /locus_tag="OO439_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6315277.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            6803..7522
                     /gene="ompR"
                     /locus_tag="OO439_000833"
     CDS             6803..7522
                     /gene="ompR"
                     /locus_tag="OO439_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6315278.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7519..8871
                     /gene="envZ"
                     /locus_tag="OO439_000834"
     CDS             7519..8871
                     /gene="envZ"
                     /locus_tag="OO439_000834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6315279.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(8947..10569)
                     /gene="pckA"
                     /locus_tag="OO439_000835"
     CDS             complement(8947..10569)
                     /gene="pckA"
                     /locus_tag="OO439_000835"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6315280.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            10948..11262
                     /locus_tag="OO439_000836"
     CDS             10948..11262
                     /locus_tag="OO439_000836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6315281.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11275..12648
                     /locus_tag="OO439_000837"
     CDS             11275..12648
                     /locus_tag="OO439_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6315282.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(12783..13661)
                     /gene="hslO"
                     /locus_tag="OO439_000838"
     CDS             complement(12783..13661)
                     /gene="hslO"
                     /locus_tag="OO439_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6315283.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(13686..14087)
                     /gene="hslR"
                     /locus_tag="OO439_000839"
     CDS             complement(13686..14087)
                     /gene="hslR"
                     /locus_tag="OO439_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6315284.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14098..14766)
                     /gene="yrfG"
                     /locus_tag="OO439_000840"
     CDS             complement(14098..14766)
                     /gene="yrfG"
                     /locus_tag="OO439_000840"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6315285.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(14831..16966)
                     /gene="igaA"
                     /locus_tag="OO439_000841"
     CDS             complement(14831..16966)
                     /gene="igaA"
                     /locus_tag="OO439_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6315286.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17286..17846
                     /gene="nudE"
                     /locus_tag="OO439_000842"
     CDS             17286..17846
                     /gene="nudE"
                     /locus_tag="OO439_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6315287.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18011..20563)
                     /gene="mrcA"
                     /locus_tag="OO439_000843"
     CDS             complement(18011..20563)
                     /gene="mrcA"
                     /locus_tag="OO439_000843"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6315288.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            20683..21462
                     /gene="hofM"
                     /locus_tag="OO439_000844"
     CDS             20683..21462
                     /gene="hofM"
                     /locus_tag="OO439_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6315289.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21462..22001
                     /gene="hofN"
                     /locus_tag="OO439_000845"
                     /pseudo
     CDS             21462..22001
                     /gene="hofN"
                     /locus_tag="OO439_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            21985..22425
                     /gene="hofO"
                     /locus_tag="OO439_000846"
     CDS             21985..22425
                     /gene="hofO"
                     /locus_tag="OO439_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6315290.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22415..22819
                     /gene="hofP"
                     /locus_tag="OO439_000847"
     CDS             22415..22819
                     /gene="hofP"
                     /locus_tag="OO439_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6315291.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRREQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            22731..23969
                     /gene="hofQ"
                     /locus_tag="OO439_000848"
     CDS             22731..23969
                     /gene="hofQ"
                     /locus_tag="OO439_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6315292.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24370..24891
                     /gene="aroK"
                     /locus_tag="OO439_000849"
     CDS             24370..24891
                     /gene="aroK"
                     /locus_tag="OO439_000849"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6315293.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            24948..26036
                     /gene="aroB"
                     /locus_tag="OO439_000850"
     CDS             24948..26036
                     /gene="aroB"
                     /locus_tag="OO439_000850"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6315294.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26128..27414
                     /gene="damX"
                     /locus_tag="OO439_000851"
     CDS             26128..27414
                     /gene="damX"
                     /locus_tag="OO439_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6315295.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27521..28357
                     /gene="dam"
                     /locus_tag="OO439_000852"
     CDS             27521..28357
                     /gene="dam"
                     /locus_tag="OO439_000852"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6315296.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28375..29052
                     /gene="rpe"
                     /locus_tag="OO439_000853"
     CDS             28375..29052
                     /gene="rpe"
                     /locus_tag="OO439_000853"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6315297.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29045..29803
                     /gene="gph"
                     /locus_tag="OO439_000854"
     CDS             29045..29803
                     /gene="gph"
                     /locus_tag="OO439_000854"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6315298.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            29796..30800
                     /gene="trpS"
                     /locus_tag="OO439_000855"
     CDS             29796..30800
                     /gene="trpS"
                     /locus_tag="OO439_000855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6315299.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            30960..31865
                     /locus_tag="OO439_000856"
     CDS             30960..31865
                     /locus_tag="OO439_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315300.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            31882..32244
                     /locus_tag="OO439_000857"
     CDS             31882..32244
                     /locus_tag="OO439_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6315301.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32328..33491
                     /locus_tag="OO439_000858"
                     /pseudo
     CDS             32328..33491
                     /locus_tag="OO439_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33491..34717
                     /locus_tag="OO439_000859"
                     /pseudo
     CDS             33491..34717
                     /locus_tag="OO439_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            34714..35592
                     /locus_tag="OO439_000860"
     CDS             34714..35592
                     /locus_tag="OO439_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6315302.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35603..35956
                     /locus_tag="OO439_000861"
     CDS             35603..35956
                     /locus_tag="OO439_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6315303.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            35968..37272
                     /locus_tag="OO439_000862"
     CDS             35968..37272
                     /locus_tag="OO439_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6315304.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37284..38369
                     /locus_tag="OO439_000863"
     CDS             37284..38369
                     /locus_tag="OO439_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6315305.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38521..39252)
                     /gene="phoB"
                     /locus_tag="OO439_000864"
     CDS             complement(38521..39252)
                     /gene="phoB"
                     /locus_tag="OO439_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6315306.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39352..40137)
                     /gene="frlD"
                     /locus_tag="OO439_000865"
     CDS             complement(39352..40137)
                     /gene="frlD"
                     /locus_tag="OO439_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6315307.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40134..40965)
                     /gene="frlC"
                     /locus_tag="OO439_000866"
                     /pseudo
     CDS             complement(40134..40965)
                     /gene="frlC"
                     /locus_tag="OO439_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41015..42037)
                     /gene="frlB"
                     /locus_tag="OO439_000867"
     CDS             complement(41015..42037)
                     /gene="frlB"
                     /locus_tag="OO439_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6315308.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42058..>43207)
                     /gene="frlA"
                     /locus_tag="OO439_000868"
     CDS             complement(42058..>43207)
                     /gene="frlA"
                     /locus_tag="OO439_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6315309.1"
                     /translation="AELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAPSLSI
                     MALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKIIPFT
                     IVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTGEIKN
                     PGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALGSTAG
                     IFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSIILQGA
                     LGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLMTTLA
                     IASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10022 a        10997 c        11863 g        10325 t
ORIGIN      
        1 agacaataga tgtatcctgg tccttgttcc gttttcaccg accacagcac atcggaatag
       61 ctttcacgca gatcgtcatc aataaagctg ctcgactcca gcttgagggt tttcatatca
      121 caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa aatcacgagc cgtgtccggt
      181 tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg aactctgctt tttgctcatg
      241 gcgttccttc acctgttgtt tgaatgccgc gactctacgc gccactccac acctgtgcat
      301 tagcgagttt tcatccctgc gagatgcttt caaaggaact tacgccgtgc tgcaagagtt
      361 gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc agacgcatac cgggctttcc
      421 aaaacaggcg atggagtaag gttaacgcag cgcccaccac tcgcgcagac aggcttttcc
      481 ttccgggcag cttttacaac tgccagagag gcagccgtca ggttcttcct gaatccgcac
      541 ggctttgccc atactttcca gttgttgcag catggcgtta atcattggct gcggagtgtt
      601 caatgtctgg cttatctggg tcgcttccat acggccccgt aacgccagca aatcacgtac
      661 ctgaataagt gaagccatta atggcaatca ccggtggtgc tggctgcgca gcaactgctt
      721 accgacttgc gggttgccag cagttcgata tccacccggc tacgcgcgcg gcgcagcaga
      781 ccgataacta cgatgttaaa caggattacc gccagaatgc acaccaggct gtaagttggg
      841 tgctgactgt agctggcgac ctgatagaac aatgttgcca gtgagtaagc gatattcagc
      901 ccccacagga tggagaagcc catccagcca cggcttgatt cacgggcgat ggctcccatc
      961 accgagatac atggtacata cagcaggacg aaaatcaggt agctgtatgc ggcagctgcg
     1021 ctaccgaatt tctgatccat cacgcccatc gccccggtac ccatttcacc gtcgcctttg
     1081 ctagcttcaa tggggttcat cagtacgcta aggctgaagg tgtctttcag gctctgccag
     1141 gtttcatcta ccgcactgaa cagctcttca ccgaggttga attccgccgg attgaactct
     1201 tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg tacccaccac cacttctttt
     1261 gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt tatcttcatg tacgccaatc
     1321 ggcttgaaga caggggttat cacccggctg acggacgcca gcgccgagtc gttgatgtta
     1381 tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc tcaggaaaat gctgacgatg
     1441 ataatcactt taccagcacg cagaacgaag cccttcagac gctgccaggt ctggataatc
     1501 aggcttttaa cgtgtggtac atggtagacc ggcagctcca tgacaaacgg cgtcgcttca
     1561 ccgcgcatga tggtgtactt gagcatcagg ccagtcagca ccgccatcac gataccgagc
     1621 atatacagcg agaaaaccgc cagggcaccg ttctgcccga agaaggcagc cgcgaatact
     1681 gcgaagatag ccagacgcgc gccgcaggac ataaacggtg ccatcatgat ggtcatcaga
     1741 cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg acggtacgtt acaaccgaaa
     1801 ccgacgatca gcggcacaaa ggatttcccc ggcaagccca gcgcctgcat caggcggtcc
     1861 atcacaaacg ctgcacgcgc catatagccg gagtcctcaa ggaaggagag gaacaggtac
     1921 atcatgccga tctgcggcac cagtggcagc acggtgttaa taccgccacc caggccctgg
     1981 gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt agccaatcca ttgaatacca
     2041 tgcacaaata gcgccacgga gccgacgtca aacagcggct gtaacgcccc gccgatgttg
     2101 atagccagca ggaacatcag gtacatcaca aagaggaaaa tcggcagacc gaggaaacgg
     2161 ttgagcacga ttttatctac cgcagtggtg aaacggctgg gttctgccgt cagggtgttg
     2221 cttaccacat cacagatggc agctatgcac tggtaacgcg catcggcaat gtgcagcgcc
     2281 ggatcatcca tttcattacg cagacgggca agggcggcat ccagatgctg cgacgcttca
     2341 ccggcgtagg cgcggctgta gatatcgcct tccagcattt gcaggcccag ccagcgacgt
     2401 tgtttcagcg ggatgtcgga aggcatcact tttgccagtg aatctgcttc gttgagtagc
     2461 ggctgtgcgt aatgcaccag ttccacattc tcgttagctt tatagcgatc aatcgccagc
     2521 ttgagcgctt caataccgcg accacgggtt gaaaccagcg ggatcactgg gcagccaaga
     2581 cgcgccgaca gagcatcaat ttcaatacga atattttgct tctcggcaat gtcgagcatg
     2641 ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt gtagcgtcag gtacaggtta
     2701 cgctcaaggt tagacgcatc caccacgtta atcagcaggt cggcgtcgcc actcaaaatg
     2761 tagtgacagg cgatttgctc atcgagcgag gtctgcgatg agatggtggt cagagaatag
     2821 gtgccgggca ggtccaccag cgtgacctga tgatcggtgg tggagaattg cccttcttta
     2881 cgttcgacgg taacgccagc ccagttacct acacgctgac gtgcgctagt gagctggtta
     2941 aataaggttg tcttgccaga atttggatta ccaattaagc caatggttaa ttttttcatt
     3001 gttgttatca ccgtattaac aggaaaccgc ttccacttct aataaggcca gatctttttt
     3061 gcgtaatacc aggctcacac gacgggtttc gatatgaatg gggtcgccga gtggagcgac
     3121 gcgcaccaca ttaaaagagg agccaggtaa catgccaaga gaaagcagtt tttggcgata
     3181 tgccgggctg atttcacggg aaaagccagt gattttccac gcagtatctg gagtgtattg
     3241 cataggtgcc tacttgtttc tcattaactg gataactacc tcatggggcg tttgacagcg
     3301 acatgagacc agatgatttg ccaacaaagg agagattaaa taatcaacaa cacaatgata
     3361 atgagaatgg tttctatcag caatacttaa aatgtgtgcg aatgttggtt tgaataataa
     3421 tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc gaaaatataa ttcgaatagt
     3481 gaattacccg gactggctaa tgtttaatta aggtttcagt tgttaatgaa gtgataaata
     3541 ttgccggaat atattatgag tttggttgtg gaataaaaag gccggagata ttctccggca
     3601 cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt gaacgcctta tcgcgcaggt
     3661 ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg ccgccgccag cgcatccatc
     3721 atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg gtttggctgc cgggcggttg
     3781 ttttgcgggc gttcattacc gccgccgcga cgggcgttgg tttcgccagg ctgctcgtcc
     3841 agacgcatgg tcagggcgat acgtttacgc tgaagatcca cttccagcac tttcaccttc
     3901 acaatgtcgc ccgctttcac cacggtatgc ggatcttcca caaacttgtt cgacaatgaa
     3961 gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga caaacgcgcc aaagttggtg
     4021 acgttggtga cagcgccttc gaggatcata cccggttgca ggtcgttcat tgtctcgacg
     4081 ccatcggcaa actgagcggt tttaaattcc ggacgcggat cgcgacccgg tttttccagc
     4141 tctttgatga tgtcagttac tgtcggcaca ccgaattttt catcagtaaa gtcagacgct
     4201 ttcaggttac gcagttcgct gctgttaccc atcagatctt tcagtgcctg ctgtgttgct
     4261 gccagaatgc gttccaccac cggataggct tccgggtgaa cggtagaggc gtccagcggg
     4321 ttatcaccgt ggttaatgcg caggaagccc gcgcactgct cgaaggcttt cggccccaga
     4381 cggctgactt tcagcagttg ctgacggttc ttgaactggc cgttctcatc gcgccaggcc
     4441 acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc gagttaatag cggaacagaa
     4501 gcggtgttga gatcgacgcc aacggcgttt acgcagtctt ctactactgc atccagtttg
     4561 cgggccagtt gcgtctggct gacgtcatgc tgatactgac ctacgccgat agatttcgga
     4621 tcgattttca ccagctccgc cagcggatcc tgcaaacggc gggcgataga taccgcacca
     4681 cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca gctcggaagc tgagtaaacc
     4741 gacgcgccag cttcgctgac gatcactttc tgtgcggtca ctttcgggaa ctgcttctgc
     4801 acgtcgagat agaaacgttc agtttcgcgg gaagctgtac cgttgccgat cgccaccagt
     4861 tcaacgttat gtttttcaca caaggcagca acggtcatcg ctacttttgc ggcttgtccg
     4921 gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag tggcatcgac cactgccact
     4981 tttaccccgg tacgcagacc cggatcgagg cccatcgttg cacgcagtcc ggcaggggcc
     5041 gccatcagca gatcgtgcag gttacgggca aagacgttga ttgcttcatc ttccgcgcgt
     5101 tcgcgcacgg tacccatcag ttcggtttcc agatgcatca gcaccttgat gcgccaggtc
     5161 cagctcacta cgcctttgcg ccagctatcc gccggggcat tgttcaggcg caggccaagg
     5221 tgatccatga tgatttgctc gcaatagctc tctttgggcg gctcatcgaa ctgcggatcg
     5281 gcattcagcg aaagctggag tacgccttcg ttacgcccac ggaacatcgc cagcgcgcgg
     5341 tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat agtcgcggaa tttcgcccct
     5401 tcctcttctt taccgctcac caccgtagaa accaaatgcg cgttcttcca cagataatca
     5461 cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt ccatcaggat atagcgcgcg
     5521 ccgtccagcg cggctttggt atctgccacg cctttatcgg catcaatata ttgcgcagcg
     5581 gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt cagccaacgg ctcaagccct
     5641 gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt tgtagggcag gtagaggtct
     5701 tcgagttcgg ttttgcttag ggtggcgttg atggccttcg ccagatcatc ggtgagtttg
     5761 ccttgctcgg aaatggactt gaggatcgcc tgacgtctct cttccagctc gcgcagatag
     5821 ctcagacgcg tttccagatt acgcagctgc gtgtcatcca gaccgccggt gatttcctta
     5881 cgataacgtg cgataaacgg cacggtattc ccttcgtcaa gcaggcgaac ggcagcgtca
     5941 acctgttccg ggcgcgcctg aatttcaccc gcaataatgc ggcagaacga atcattcatc
     6001 atggtttgag ttcatctttt cggatcaaaa atcaggggat agttatacgg actggctggc
     6061 aaaaatgcca gccatcggca ggaggttaag actcttcatt acggtttcac gtattcgata
     6121 gcattaacat accagctcgc ttccccggca ggggtattca ccaccgccag atcgccgact
     6181 tcttttttca gcaatgcgcg ggccatcggg gaatcgatag agatgtaatc tttacggcca
     6241 aaaatttcat cgtagccgac aatacggaaa cggtgagtca cgccatcgtc gttttcaatc
     6301 tccacccacg cgccaaaaaa gactttgcct tcctgctgag gggagtaatt gacgattttg
     6361 agattttcca tgcatttagt gagatagcgc acgcgacggt cgatttcacg cagacgcttt
     6421 ttattatact gatagtcagc attttcgctg cggtcgccca gacttgcggc ccaggtcacc
     6481 ttttttgtga cctccgggcg ttcttcacgc cagagataat taagctcttg tttgagtttt
     6541 tcataccctt cccgggtaac caggggcgtt ttcatctcgt tgattccctt tgtctgtttg
     6601 ataatgcgca cattgggtat aacgtgatca tatcaacaga atcaataatg tttcgccgaa
     6661 taaattgtat acttaagctg ctgtttaata tgctttgtaa caatttaggc tgaaattcat
     6721 accagattta gctggtgacg aacgtgagct tttttaagaa tacacgctta caaattgttg
     6781 cgaacctttg ggagtacaaa caatgcaaga gaactacaag attctggtgg tcgatgacga
     6841 catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa caaggcttcc aggttcgaag
     6901 cgtcgctaat gcagaacaga tggatcgcct gctgactcgt gaatctttcc atcttatggt
     6961 actggattta atgttacctg gtgaagatgg cttgtcgatt tgccgacgtc ttcgtagtca
     7021 gagcaacccg atgccgatca ttatggtgac ggcgaaaggg gaagaagtgg accgtatcgt
     7081 aggcctggag attggcgctg acgactacat tccaaaaccg tttaacccgc gtgaactgct
     7141 ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa ctgccaggcg caccgtcaca
     7201 ggaagaggcg gtaattgctt tcggtaagtt caaacttaac ctcggtacgc gcgaaatgtt
     7261 ccgcgaagat gagccgatgc cgctcaccag cggtgagttt gcggtactga aggcactggt
     7321 cagccatccg cgtgagccgc tctcccgcga taagctgatg aaccttgccc gtggtcgtga
     7381 atattccgca atggaacgct ccatcgacgt gcagatttcg cgtctgcgcc gcatggtaga
     7441 agaagatcca gcgcatccgc gttacattca gaccgtttgg ggcctgggct acgtctttgt
     7501 accggacggc tctaaagcat gaggcgattg cgcttctcgc cacgaagttc atttgcccgt
     7561 acgttattgc tcatcgtcac cttgctgttc gccagcctgg tgacgactta tctggtggtg
     7621 ctgaacttcg cgattttgcc gagcctccag cagtttaata aagtcctcgc gtacgaagtg
     7681 cgtatgttga tgaccgacaa actgcaactg gaggacggca cgcagttggt tgtgcctccc
     7741 gctttccgtc gggagatcta ccgtgagctg gggatctctc tctactccaa cgaggctgcc
     7801 gaagaggcag gtctgcgttg ggcgcaacac tatgaattct taagccatca gatggcacag
     7861 caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca aaagttcgcc tgtcgtctgg
     7921 ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc cgctgaccga aattcatcag
     7981 ggcgatttct ctccgctgtt ccgctatacg ctggcgatta tgctattggc gataggcggg
     8041 gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg atctcgaaca tgctgctttg
     8101 caggtcggta aagggattat tccgccgccg ctgcgtgagt atggcgcgtc cgaggtgcgt
     8161 tccgttaccc gcgcctttaa ccatatggcg gctggtgtta agcaactggc ggatgaccgc
     8221 acgctgctga tggcgggggt aagtcacgac ttgcgcacgc cgctgacgcg tattcgcctg
     8281 gcgactgaga tgatgagcga gcaggatggc tatctggcag aatcgatcaa taaagatatc
     8341 gaagagtgca acgccatcat tgagcagttt atcgactacc tgcgcaccgg gcaggagatg
     8401 ccgatggaaa tggcggatct taatgcagtg ctcggtgagg tgattgctgc cgaaagtggc
     8461 tatgagcggg aaattgaaac cgcgctttac cccggcagca ttgaagtgaa aatgcacccg
     8521 ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg ccgctcgtta tggcaatggc
     8581 tggatcaaag tcagcagcgg aacggagccg aatcgcgcct ggttccaggt ggaagatgac
     8641 ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc agccgtttgt ccgcggcgac
     8701 agtgcgcgca ccattagcgg cacgggatta gggctggcaa ttgtgcagcg tatcgtggat
     8761 aaccataacg ggatgctgga gcttggcacc agcgagcggg gcgggctttc cattcgcgcc
     8821 tggctgccag tgccggtaac gcgggcgcag ggcacgacaa aagaagggta aataaacggg
     8881 aggcgaaggt gcctcccgtt ttgctttcta taagatactg gatagatatt ctccagcttc
     8941 aaatcattac agtttcggac cagccgctac cagcgcggca cccgcagggg tgtcggtgta
     9001 tttatcgaag ttgtcgataa acagtttcgc cagggtttcg gctttttcct gccactgttc
     9061 cggagaagcg taggtgttac gcggatcgag aatcttcgtg tctacgcccg gcagttcggt
     9121 tgggatcgcc aggttaaaca tcggcagagt gaaggtttct gcattatcca gcgaaccgtt
     9181 gaggatggcg tcgataatgg cgcgggtatc tttaatcgag atacgtttgc cagtgccgtt
     9241 ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc tgcatacgtt tcaccagcac
     9301 ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg ccgaagcaag cggagaaggt
     9361 tggcgtcggt tcggtgatgc cacgctcagt accggccagt ttggcggtga agccagagag
     9421 gaagtgatac tgggtttgat cggcagtcag gcgagaaacc ggcggcaaca cgccgaaagc
     9481 atcagcagtc aggaagataa ccttagtcgc gtggcccgct ttggaaaccg gcttaacgat
     9541 gttatcgatg tgatagattg gataagaaac gcgggtgttc tcggtttttg aaccatcatc
     9601 aaagtcgata gtgccatctt cacgcacggt gacgttttcc agcagcgcat cacgacggat
     9661 agcgttgtag atttcaggtt ccgcttcttt cgacagcttg atagtttttg cgtagcagcc
     9721 gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt tcgtcatcgc caatcaggcg
     9781 gcgtttcggg tcggtggaaa gggtggtttt accggtgccg gaaaggccga agaacaccgc
     9841 aacatcgcct ttctcgccaa cgttggcgga gcagtgcata gaagcgatac ctttcagcgg
     9901 cagcaggtag ttcatcatcg agaacatccc tttcttcatt tcgccgccgt accaggtgcc
     9961 gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg aagttttcgg agttcagacc
    10021 ctgttctttc cactgcgggt tagtgcactt cgcgccgttc ataacgataa agtctggttt
    10081 gaaacctgcc agctcttcat cgctcgggcg aataaacatg tttttgacaa aatgcgcctg
    10141 ccaggccact tcggtgatga aacggacgga aagacgagta tccgggttcg caccacagaa
    10201 agcgtcgaca acgaacagac gtttgccgga aagctgcctg gtcaccaggc ctttcagatg
    10261 ctgccaggtt tccggagaga gaggtttgtt gtcgttctta cctttgcctt tgtctgccca
    10321 ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac ttatcttttg gtgaacgacc
    10381 ggtgaagatc ccggtatcga cggcaacggc acccagatta gttaacaccc cgcgctcata
    10441 acctgtcagg ctcggatcga gctcttcctg atacagcagg tcgtagcttg ggttgtaaac
    10501 gatatcatgt acgtcactga taccataagc ctcgagttct tgcggggtca aaccattgtt
    10561 aacgcgcatt tcactgctcc ttagccaata tgtattgcct gaatagtaaa gtctttttgg
    10621 gggtgttaac cgcgacaagg ctcatagatt tacgtatctg gagaaattca tatttgacaa
    10681 aaaactccgt gattcctgtc acgaaacggt tgctattatc gcagaaatag cattttaggt
    10741 gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg gcgggaatat tgtgagtgga
    10801 atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta aagtatcgat tcaccacgct
    10861 aacttttttt gattattttc tgaaaaaagc aaaatactac tgcccggatg cgataattaa
    10921 cccacctttt gattctggat aaccaacatg gataacgttg aactttcacc ggcaacacgc
    10981 tgggggatga ttgctaccgg attacttcag gggctggttt gctacctgct tatcgcctgg
    11041 cttgccggaa aaaatcacag ttggattgtt tatggcgtgc ctgcgaccgt cgcgttttca
    11101 tccgttttac tcttctccgt gatctctttt aaacagaagc gcctttgggg atggctggcg
    11161 ctggtgttta ttgccacgac agggatgagc ggttggctga agtggcaaac tgatggcatg
    11221 aacccctgga gagctgaaaa ggcgatttgg gattttggct gatatctgct gttgatggca
    11281 atgttgttgc taccgtggat acaacaaagc cttcgtatcc gcaatggcag tagccgctat
    11341 agctactttt accagtcagt atggcataac gtacttatat tactggtgat ttttctctcc
    11401 aatggcctga cgtggctggt gcttttactg tggggtgagt tgtttaaact tgttggcatc
    11461 acgtttttca atacgctctt ttttgcaacc gactggttta tgtatcttac atttggtctg
    11521 gttaccgcac tggtggtgat cctcgcgcgt acacagtcac gtttaatcga ctctattcaa
    11581 aagttgttca cgctaatcgc cacggggttg ctgccgttag tatcattgct aaccctgatg
    11641 tttatcatca ccctgccgtt tacggacctg agcgcgattt ctcgccacat ctccgccgcc
    11701 gggttgctgt taacgctggc ctttttgcaa ttgatcttaa tggctattgt ccgcgatccg
    11761 caaaaagcgt cacttccctg gacggggccg ttgcgttgcc tgattaaaac cgctttgctg
    11821 gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat ggctgcgggt cgctcagtac
    11881 ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc tggtgttact ggtgtggtcg
    11941 ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa atccccttgt tcttcagggc
    12001 aaagtgaacc ttgcggtttc gttattggtg ttggtgatac tggtgcttct taattcgccg
    12061 gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg cgcgttatca gagcggcaaa
    12121 aacacgccag accaggtaac tatctacatg ctcgaacaga gcggtcgcta tggacgtgcg
    12181 gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag acccgaaacg cgcgcgggat
    12241 ctgctgatgg cgttagatgg agagcaacat cttcaggaac aggtatcgga aaaagtatta
    12301 gccgaaaatg tgttaattgc tcctggttct gttaaaccgg atgcgacatt ctggtcggcc
    12361 ttaatccagg atcgctataa cgtgatgacc tgtattgaaa aagacgcctg cgtcctggtc
    12421 gagcaagatc tgaatagtga tggtcaggcg gagcggatcc tgtttgcttt taatgatgac
    12481 agagtcattg tctatggctt tgactcagac agaaaagaat gggacgcgct tgatatgagt
    12541 ttacttccga acgaaataac gaaagaaaaa ttactcacag ctgcgaagga tggaaaactg
    12601 gatctgacgg tagatggtga aacgctggaa ataaacttaa gtaagtgaca actcttttaa
    12661 cggatacgac gctgtatctg accaacaata atttgcgatt tatttcacag gcctggtaag
    12721 cgcagcacat caggccatta cgtcgtaaaa tccggcagag ccctctctgc cggacatact
    12781 cattaatgaa cttgcggatc tgccggagac gcgttgttgc ggatttcagc aatatccatc
    12841 gcattgaaca gatagtggtt accgcagtaa tcacaatgca tgtcaatttc gccatcttcc
    12901 gccaggatgc tatcgacttc ttcatcaggc agcgttttca gcgcgtcggc gcaacgttca
    12961 cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt aaaccgtcac ctcttcttcg
    13021 tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca gcagttcttc ggttttgatg
    13081 gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct gggcattttg cgcaggcatt
    13141 acctgcaaca acataccgcc tgcagccggt ttgccgtcta cgtcgccggt gcgaataaac
    13201 aggcgcgtcg gcagctgttc agaacgcata aagtaatctt ccaggcaggc cgccagggta
    13261 tcaccttcca gaccaaccac gccctgatag cgttcgcctt cgctcggggt aatggtgatc
    13321 accacgtaac cattgccgac cagcgttttc aggtcggcat tttctggaat ttcgccctgc
    13381 acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt taataaccgc cagattcatc
    13441 ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa acttcagcgt agcggttaac
    13501 aggctggtcg caaccagcag ttctgccagc acgtttttaa cgggctgcgg ataatcgtga
    13561 ttctcaagga tctgttgcag ggtttccgaa acggttacca gttcgccgcg cacggcaaag
    13621 ttttcaaaca gatagcgatg taattggtca tgttgcggca taatcatctc tcttgcaggt
    13681 gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt cgcggcgctc ttttttgtcc
    13741 gggcgtcggt ccgggtgcgg catggttaag gcattaagtt tacgtgccag cgccattttt
    13801 tcgcgtttct ctacactttc cgcagtctct tcatacagca aggctgcctc gctggcgggg
    13861 cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt cgtcatttcc ctggcgcaga
    13921 gtgagcgtgg cattcagctc gacgattttg ctcggcttgc tgcgctgccc gttgtaatgc
    13981 accttaccgc cttcaatcat ttcacgggcc agcgcgcggg ttttataaaa acgggcagcc
    14041 catagccatt tatccagtcg aacctcaaca gcaggttttt ctttcatggc gtctccttca
    14101 cattagcgag gggatcaggc ggcggtagtc attcagtgac ggatggcgtt gatactgttt
    14161 ctcggcaatc ccggaatcag gattagtcac gccgaggcag taacgaatac caaattgcgc
    14221 ggcagcatcg agaatcgctt cgctgtcatc aataaacagc gttctttcag ctttcagacc
    14281 cgtagcttcg gccaccgcat gccataaccg ctgatcctct ttcggataac caaatgtgtg
    14341 ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta tgctcaagtt ttaccgccag
    14401 gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta ccgctggctt tcagtgcctc
    14461 aagaaacgga atggtatctt cacgcagtac ggcacgcggt cccatctcgg tggtcatcgc
    14521 acagatatcc agacccagtt gctcactcca gtaatcaaga cagtaccagt ttagcgtatg
    14581 ctgtacgtcg tgatattgct ggcgcatata ttccatcgct tcctgtggcg taaccccgtt
    14641 tttcgcgccc catgtttcag gcaccagctt ttgccagaaa tagttatcga aggcgaggtc
    14701 gagcaacgtg ccgtccatat ccagcagaac ggtatctacg tcctgccagg caatgttgat
    14761 atgcatgagg gaaatctcca gagtgaagca atttgcgcga cagggtagca taacctgccg
    14821 cgcaaacgtg ttattcgata aggctttctg aaggggtgat cagttgcggg ttcaggcagc
    14881 tttcataata ggcctgaatc ttcatcatgc gagtgcgatg acgctggtaa cgacgccagg
    14941 cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag tagcagcaat gtggtgctga
    15001 gatagcgcca caggccggaa cgatccggga ttggatgaag gccaatatgc tgcgtaccat
    15061 tggcgtcagt gaagattttt gtgacgatac cctcggcgtt aaacggcgta tgcatcagca
    15121 tttgtgccag tttctggaaa gcgttccact gttcttgcgg cgggtagtcg taaagtgatg
    15181 ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc gctgacaatc aggaatccgc
    15241 cgggggccgg actgtttagt gattgtgccg ctcgcgccgt ttcatgcgtg ataaacggcg
    15301 cggtggaggt tgccaccagg ttatccagcg attccgcact caccgggcgt aataacacat
    15361 tcacgccatc gagcttcccg gcgttggcgc gttttaccag cgcgtcccag tctttactgt
    15421 tgccgagatt gaccagcgca tttttcagtc gcacacagtc atctttggca gaacataaat
    15481 ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa taccatgccg gatttttgaa
    15541 ttgctgaacg taatgaggca ctgacgcgag atccatcttc cggtttaggg tgcagctggc
    15601 gattaactgc ttcagtcaat gccgtcgctt tgttgaccag ttcagattct ggtaatggca
    15661 atgagcgggc gtcgttccag atgatctgcg agcagtcaaa cggtaaaaaa ggtgaattgg
    15721 ttttcgcgct ccaggttccg gaagtgcgaa tattacacat tcccgtaccg ctgatacgca
    15781 atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt tacgctggtg gcttcaatgg
    15841 tctgtgcgcc tttcatccat gagagagtga atttcagcgg catatccagc gggatccaga
    15901 ataacagcat aaacagcacc agcagcgagc ccgcagcgat aatcgtactg cgcagccagt
    15961 gctgtaacgg aaagtttttt acttcatcat gcagcgaaag atatcgcccc tgacgcacta
    16021 cgtggcggtc gagatagata tcaatatcgg tttgttgacc gagatcctga gcaatgtatg
    16081 gctgccagtg tgcgggatag accaggtcga taataccgag tgaaatattg ttgatctgtt
    16141 cctgatcgtt ttcgccaaac aatccccaac gacggggtgt accgcgcaga caatgaattt
    16201 cccgcaggga ggattttgcc gggggcgcga atagccccca cagacctgcg cccagcagca
    16261 gtaatgcgcc gcctgccagc cacggaacaa atacatccgg ggtaatcagg cagaaaaaga
    16321 acatcaggaa ggaggcgacg atcagcaacg cttcacgcag cccgcgcgga cgactcagcg
    16381 catattcttc atgcgtttct ttgcgaatat tgagtagctc gatttgctca ctctcttccc
    16441 cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc atagccgcgc gtttcctgca
    16501 tgtactcctg cagcgtatga ccgttgagtg aaataaccag cggcagcgaa tcggtataaa
    16561 tcagttcaac ggtattctca tcgttgatgt attgttccca gaaagggggc aggtggactt
    16621 ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc ggtagagatg ccgtaacgcg
    16681 tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc attcagcgcc agagagatcg
    16741 gtgccgcgct agctcccgtt gggccaggca cctgtaatac ctgcgtcagg ctctcaagat
    16801 aattttcaac ggcgctacgt tcttcaggtg tgagtttacg cgtttgcgca tccgcgaagg
    16861 cgttggtcca gggcagctga cgccgtctgg atcgcacttt tatcagccat cccgcaagta
    16921 gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt gctcatgctt tccccatctt
    16981 acttagtctg tcaggcgtgg tcagtcgcac cagacacaat acatgattct gcggttggct
    17041 atcggcaagc gtggagttta acttgagcat cattaagcac atcacagtaa ccgctgatca
    17101 tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg agttatttca acctattgac
    17161 ttttaatttg atttgaggac gattatttat taagttacat aaatgtaaat aacatgcaat
    17221 tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg ctgatttcac agcatagatc
    17281 ccacgatgag caaatcatta caaaaaccca ccattctgaa tgttgaaact gtagcccatt
    17341 cccgactgtt taccgtcgag agcgtggatc tggagttcag caatggcgtg cggcgtgttt
    17401 atgaacgaat gcgtccaacc aaccgggaag cagtgatgat tgtgccgatt gtggacgatc
    17461 acctgatcct gatccgcgaa tacgcagtgg gaactgaatc ctacgaatta ggtttttcga
    17521 aaggattaat tgatccgggt gaaagcgtct acgaagccgc taaccgcgag ctaaaagaag
    17581 aggttggatt tggtgcgaac gatctgactt ttttgaagaa gctcagcatg gcaccgtctt
    17641 acttttccag caaaatgaat atcgtggtcg cgcaagatct ctacccggaa tcgctggaag
    17701 gcgatgagcc agagccgcta ccacaggtgc gctggccgct ggcgcatatg atggatttgc
    17761 tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc gctgttcctc gtgcgcgaat
    17821 ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg cctgatgcac aacgattatc
    17881 aggcttatgg ggttcctgca atatattgat ttttcacgat tttgttagcc ggataacgcg
    17941 ttcacccgca tccggcataa acaaatgcac tttgtcagcc aactgaaaag gcgccgaagc
    18001 gcctttttaa tcagaacaat tcctgtgcct caccattatc gataatggtt gttcccacct
    18061 cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg cggctgttgc
    18121 caccattagc taactgcccg gtgctgcgat cgatattcac cgtcacaata cccggaggcg
    18181 gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt catataagcg tcccatgcag
    18241 gctgggcact cttggcaccg ccttcgtaac ctgagatctg atctttaatc gctccggaag
    18301 ccgttgtatg accgagatta cgacggtgat catcaaagcc aatccagacc gaggtcacaa
    18361 cgcccggacc gtaacccgag aaccacgcat ctttcgaact gttagtggta ccggttttcc
    18421 cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca gccggtaccc tgccagcctg
    18481 gttcaccaaa gatattggtg ttcaaagcac tcttaatcag gaatgccagc ggagtgttga
    18541 tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac taacgcctga tttgcctgct
    18601 ccagctgcgg cattggtaca gaaacattct gctgctcgcg ggagatagcg acatcttcaa
    18661 catcgttatt ttccagcacg ttcgatttct gcgtatcacc gtaaatcacc ggaatatcgc
    18721 attccgggca ggctactttc ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa
    18781 ttttgctgat aaaccacggg tccaccagga agccgccgtt cgccatgacc gcgtagccgc
    18841 gcgccacctg cattggggtg aaggacgctg aacccagcgc cagcgattcg gtgtggacaa
    18901 tgttttgcgc cgggaagccg aagcgttgca gatattctgc agcgtagtcg acgcccatcg
    18961 cccgcattgc acgtaccatc accacgtttt tcgactgacc cagcccctga cgtaagcgaa
    19021 ttggaccagc atactgcggt ggtgagttct tcggctgcca gtcagaaccg acacctgcat
    19081 cccagcgaga aattggcaca tcgttcaaca tacttgccag cgtcagacct ttatccatcg
    19141 ccgcggtgta gaggaacggt ttgatgttgg aacccacctg acgcagtgcc tgggtggcgc
    19201 ggttaaactt gctctgattg aaatcaaagc caccgaccag cgccataacg gcaccatttt
    19261 gcggattgat cgacaccagc gccgagttca cttccggcac ttgtgccagc caccatgcat
    19321 cgccaacctg acgaacccag atttgctgac ccgtttgcag aacatcggtc actttacgcg
    19381 gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc ccagcgaacg ccgtccatac
    19441 tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc ttcctgagga ttggcgctgg
    19501 tgactgcggc aggcagcagc ggaccatagg tcggcagcgc cttcagcgta tcggtaatct
    19561 tgttgttatc ccacgccgac tcgcccactt tccacagcac atttgccggg ccgcgatagc
    19621 cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc ctgctgtgcg gcctgctgca
    19681 ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc ataggcactt tcgccataac
    19741 ggttatacat ctcctggcgc accatttcgc tcaggtacgg cgcagagaaa gcaatctccg
    19801 gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg atcgaactgt tgttgggtga
    19861 tatacccttc atccagcatc cgcgacagta cgacgttacg ccgcgcgacg gcacgatcca
    19921 ttgagtagag cggattaaag gtggaaggcg ctttcggcag cccggctatc acagccattt
    19981 cgttcagcgt cagttggtcg accgttttac cgaaatagac ttgtgccgca gcaccgacac
    20041 cataggcgcg gtaaccgagg taaatcttgt tcagataaag ctcgaggatc tcgtctttcg
    20101 tcagcaactg ttcaatgcga atcgcgagga agacttcctt aatcttacgc atcagcgtgc
    20161 gttctggact gaggaagaag tttctcgcca gctgctgggt aatggtactc gccccttgtg
    20221 acgcgtggcc ggagaacagc gccacacttg ccgcacggaa gatccccacc gggtcaacgc
    20281 cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc atctccggcg
    20341 ggatttgatc caacgtaacc ggaatacgac gtttctcacc gtattgagca atcagctcgc
    20401 catcggcgct gtaaatctgc atcggaattt gcaggcgaac atcttttaat gtcgccacat
    20461 ccggcagttg tggctcgatg tagcggtata ggccataaat cgagcctgct cccagcagaa
    20521 tgcaacagac tgcaaggatc aaaaaatact ttacgaactt cactggaaat ttcccattta
    20581 gtttcatttg ggcagtttat aaacaaacgt gcggtagtat aaaggcaagc cagacgcatt
    20641 gatatacccg tcagagtgac gggtgataag gagatcatca caatggcatt taagatctgg
    20701 caaattggtt tgcatttaca acagcaagaa gcagtagcgg ttgcgatcgt gcggggcgca
    20761 aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg agaacgacat tatcaaagat
    20821 gggcggattg ttgatgcgca gcggctggct aaaacgttgt taccgtggag tcgcgaactg
    20881 ccgcagcgtc atcacattat gttggcgttt cccgccagtc gcacattaca gcggtcattt
    20941 ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg cctggctgtc agggacgatg
    21001 gcccgcgagc tggatatgga tccggactcc ctgcgtttcg attacagcga agactcactc
    21061 agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct actcactctg
    21121 gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg atgccagtgc attacagcga
    21181 ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg
    21241 ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg tggggatgac tagcgcgaag
    21301 gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg cgatatgtgg cgaaggcgga
    21361 tttgatccct gggaggccgt ttctgttcgt cagccgccgc taccgccgcc aggtggagac
    21421 tttgccatcg cgctggggct ggcgcttggg aaggcgtact gatgaacccg ccaattaatt
    21481 ttttgccctg gcgacagcaa cgccggaccg cttttcttcg tttctggttg ctgatgttcg
    21541 ttgcgcctct gctgctggcc gtcgggataa cgctaatact gcgtctgaca gacaacgccg
    21601 aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca actcgcccgc agcttacaga
    21661 taacgaaacc acgtttgctg gagcgccaac aaattcgtga gcagcgtttg caacggcagc
    21721 gccagcgaca atttacccgc gactggcaat ctgcgctgga agcactggcg gcgcttttac
    21781 ccgagcacgc ctagctgaca acgataagct ggcagcaggg aacgctggag atcaaggggc
    21841 tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc actccgccag gatgcgtctt
    21901 ttcatctcaa tcagcgggga gccacgcagc aggatgcgca gggacgctgg caatttgagt
    21961 atcagttaac aaggaaggtt agcgatgaac atgttctttg actggtggtt cgccacatca
    22021 ccccgcctcc gccagttttg ctgggcattc tggttgctga tgttagttac gctcattttt
    22081 ctgtcatcga cacaccatga agagcgcgac gcattaattc gactacgggc aagtcatcac
    22141 cagcagtggg ccacactgta tcgcctggtt gacaccactc ccttcagcga agaaaaaacg
    22201 ctgccctttt cgccactgga ttttcagttg cccggcgcgc aactggtttc ctggcatcca
    22261 tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg aagcagtgcc gtcggcattt
    22321 acacggctgg cagagcgcaa cgtcagcgtg agccgttttt cattaagcgt ggaaggtgat
    22381 gatcttttgt tcacgctaca actggagacg ccgcatgagg gttaaacgct ggttgttggc
    22441 aggtattgta ttgtgccttt taaccggtat gcgtgaccct tttaaaccgc cggaagatct
    22501 atgccggatt agcgaactta gccagtggcg ctatcagggg atggtagggc gaggcgagcg
    22561 catcatcggt gtaataaaag acgggcaaaa gaaatggcga cgggagcagc aaaacgatgt
    22621 gctggaaaac ggctggacaa ttttacagct gacgccagac gtactaacgc tgggtaccgg
    22681 gacaaactgc gaaccgccac aatggttgtg gcaacggcaa ggagatacaa atgaagcaat
    22741 ggatagccgc actactgttg atgctgatac ccggcgtaca ggcggcaaag ccgcaaaaag
    22801 tgacgctgat ggtggatgac gttccggtag ctcaggtgtt gcaggcgctg gctgaacagg
    22861 agaagttgaa cctggtcgtg tcgccagacg tcagcggtac ggtgtcgtta catctaacag
    22921 atgttccctg gaagcaggca ctacaaactg tagtgaaaag cgccggactg ataacgcggc
    22981 aggaaggcaa cattctctca gtgcattcca ttgcctggca gaataacaat atcgcccgcc
    23041 aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct ggaaaatcgc agtataaccc
    23101 tgcaatacgc cgacgcggga gaactggcga aagcggggga gaagctactg agtgccaaag
    23161 ggagtatgac cgtcgataaa cgcaccaatc gccttttgct acgagataac aaaacggcgt
    23221 taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc ggtcgggcag gttgagctgt
    23281 cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga gttaggcgtg aaatggacgc
    23341 tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac cacgcttggt agcgacctct
    23401 ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg gcgcatcaac ggacgcttgc
    23461 tggatcttga gctttccgcg ctcgaacaaa aacagcagct ggatattatc gccagtccgc
    23521 gtctgctggc ctcacatctt cagcctgcca gcattaaaca ggggagcgaa attccatatc
    23581 aggtttccag cggggaaagt ggcgcgacgt cggtggaatt taaagaggcc gtcctgggga
    23641 tggaagttac gcccacagtg ttacaaaaag gtcgcatccg gctgaaatta cacatcagcc
    23701 agaacgttcc ggggcaggtg ctacagcagg ccgatggcga agtgctggcg attgataagc
    23761 aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac gttggcgctg ggcggcattt
    23821 ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt gcttggcgac attccctggt
    23881 tcgggcaatt atttcgtcat gacggaaaag aagatgaacg acgcgagtta gtggtgttta
    23941 tcacgccacg actggtttcc agtgagtaaa cagccgtaaa agcggtaatg tttttacgct
    24001 gaacgtgttt catctatttg acgcgcgcag gtatttagca tacaaggagt accgatttga
    24061 gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat ttatcattca ggcggcattt
    24121 tgctgtcttt tttacgctaa tcttacccgg tgatttatcg ccagagcggt ggtagcaagg
    24181 cagcgcgctt gcagcgacca gatatgcaga gggatgggtg atttattcag ttgccaaacc
    24241 cgctggagta ttgagataat tttcagtctg actctcgcaa tatcttatga ggtttcagtt
    24301 catgtcctgc ggcgctctct gagcgaagcg ggtttatcat taacgaatag tcttagtagt
    24361 accgaaaaaa tggcagagaa acgcaatatc tttctggttg ggcctatggg tgccggaaaa
    24421 agcactattg ggcgccagtt agctcaacaa ctcaatatgg aattttacga ttccgatcaa
    24481 gagattgaga aacgaaccgg agctgatgtg ggctgggttt tcgatttaga aggcgaagaa
    24541 ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga ccgagaaaca gggtattgtg
    24601 ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc gtaaccgtct ttccgctcgt
    24661 ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac ttgcacgcac gcagcgtgat
    24721 aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg aagttctgga agcgttggcc
    24781 aatgaacgca atccgctgta tgaagagatt gccgacgtga ccattcgtac tgatgatcaa
    24841 agcgctaaag tggttgcaaa ccagattatt cacatgctgg aaagcaacta attctggctt
    24901 tatatacact cgtctgcggg tacagtaatt aaggtggatg tcgcgttatg gagaggattg
    24961 ttgttactct cggggaacgt agttacccaa ttaccatcgc atctggtttg tttaatgaac
    25021 cagcttcatt cttaccgctg aaatcgggcg agcaggtcat gttggtcacc aacgaaaccc
    25081 tggctcctct gtatctcgat aaggtccgcg gcgtacttga acaggcgggt gttaatgtcg
    25141 atagcgttat cctccctgac ggcgagcagt ataaaagcct ggctgtactc gataccgtct
    25201 ttacggcgtt gttacaaaag ccgcatggtc gcgatactac gctggtggcg cttggcggcg
    25261 gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta tcagcgcggt gttcgtttca
    25321 ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc cgttggcggc aaaactgcgg
    25381 tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta ccagcctgct tcagtggtgg
    25441 tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt agcgtcgggg ctggcagaag
    25501 tcatcaaata cggcattatt cttgacggtg cgttttttaa ctggctggaa gagaatctgg
    25561 atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg tattcgccgt tgttgtgaac
    25621 tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg gttacgtgct ttactgaatc
    25681 tgggacacac ctttggtcat gccattgaag ctgaaatggg gtatggcaat tggttacatg
    25741 gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac gtcggaacgt ctcgggcagt
    25801 ttagttctgc cgaaacgcag cgtattataa ccctgctcaa gcgggctggg ttaccggtca
    25861 atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca tatgctgcgt gacaagaaag
    25921 tccttgcggg agagatacgc ttaattcttc cgttggcaat tggtaagagt gaagttcgca
    25981 gcggcgtttc gcacgagctt gttcttaacg ccattgccga ttgtcaatca gcgtaacaac
    26041 aagaaaggtc aggccgctta tcaagcggtc tattagcttc aggttaattg caacgtggta
    26101 agcattaacc ttttagtggg gtgttaaatg gatgaattca aaccagaaga cgagctgaaa
    26161 cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat cttctgaacg ttctgagcgt
    26221 actgaacgtg gcgaaccgca gatcaatttt gatgatattg aacttgatga cactgacgat
    26281 cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac cggaaatcga agaagaaatt
    26341 gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag agcgtcgtcc gcgtaagcgc
    26401 aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga tgatgggcgt cggcattctg
    26461 gttctactgc tgttgatcat cggtatcggt tctgcgctaa aagccccctc gacctcttcc
    26521 agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg caggcaatgc gaccgatcag
    26581 gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg aaaatactca gcaggatgtt
    26641 tctctgccac cgatctcttc tacgccgact caagggcaaa ccccggcggc aacggatggt
    26701 caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc tgacccagcc acaaaatcag
    26761 caacagttga acaatgtggc ggtcaattcc acattgccga ccgaacccgc gactgtcgcg
    26821 cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa cgcaaaccgc tgaacgtccg
    26881 tccactacgc gcccagctcg tcagcaggcg gtgattgaac cgaaaaaacc gcaagcaacc
    26941 gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc gtactgaacc agctgcccct
    27001 gtggcgagca cgaaggcacc gactgcgact tctacgccag caccaaaaga gacggcgact
    27061 acggctccag tacagacggc atccccggcg caaaccacgg caacaccagc cgctggaggg
    27121 aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt ccagccatta cactctgcag
    27181 ctgagcagtt cctctaacta cgacaacctg aacggttggg cgaagaaaga gaatctgaaa
    27241 aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt ggtatgtcct ggtttctggc
    27301 gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta cattgccagc agatgttcag
    27361 gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg ccgatctgaa gtaatcaagg
    27421 ttatctcccg caatggttta tcgttgcggg agctgcctga agcgctggat gctgtcggag
    27481 ctttctccac agccggagaa ggtgtaatta gttagtcagc atgaagaaaa atcgcgcttt
    27541 tttgaagtgg gcagggggca agtatcccct gcttgatgat attaaacggc atttgcccaa
    27601 gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg gtgtttctca acaccgactt
    27661 ttctcgttat atccttgccg atatcaatag cgacctgatc agtctctata acattgtgaa
    27721 gatgcgtact gatgagtacg tacaggccgc acgcgagctg tttgttcccg aaacaaattg
    27781 cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa agccaggatc cgttccgtcg
    27841 ggcggtactg tttttatatt tgaaccgcta cggttacaac ggcctgtgtc gttacaatct
    27901 gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa acctatttcc cggaagcaga
    27961 gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc tattgtgagt cttacgccga
    28021 tagcatggcg cgcgcagatg atgcatccgt cgtctattgc gatccgcctt atgcaccgct
    28081 gtctgcgacc gccaacttta cggcgtatca cacaaacagt tttacgcttg aacaacaagc
    28141 gcatctggcg gagatcgccg aaggtctggt tgagcgccat attccagtgc tgatctccaa
    28201 tcacgatacg atgttaacgc gtgagtggta tcagcgcgca aaattgcatg tcgtcaaagt
    28261 tcgacgcagt ataagcagca acggcggcac acgtaaaaag gtggacgaac tgctggcttt
    28321 gtacaaacca ggagtcgttt cacccgcgaa aaaataattc tcaaggagaa gcggatgaaa
    28381 cagtatttga ttgccccctc aattctgtcg gctgattttg cccgcctggg tgaagatacc
    28441 gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg acgtcatgga taaccactat
    28501 gttcccaatc tgacgattgg gccaatggtg ctgaaatcct tgcgtaacta tggcattacc
    28561 gcccctatcg acgtacatct gatggtgaaa cccgtcgatc gcattgtgcc tgatttcgct
    28621 gccgctggtg ccagcatcat tacctttcat ccagaagcct ccgagcatgt tgaccgcacg
    28681 ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg tatttaaccc ggcgacacct
    28741 ctgagctatc tggattacgt gatggataag ctggatgtga tcctgctgat gtccgtcaat
    28801 cctggtttcg gcggtcagtc tttcattcct caaacactgg ataaactgcg cgaagtacgt
    28861 cgccgtatcg acgagtctgg ctttgacatt cgtctggaag tggacggtgg cgtgaaggtg
    28921 aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt tcgtcgccgg ttcggcaatc
    28981 ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc gcagtgaact ggcaaaggta
    29041 agtcatgaat aagtttgaag atattcgcgg cgtcgctttt gatcttgatg gtacgctggt
    29101 cgacagtgct cccggtcttg ctgctgcggt agatatggcg ctgtatgcgc tggagttgcc
    29161 catcgcaggt gaagaacgcg ttattacctg gattggtaac ggcgcagatg ttctgatgga
    29221 gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag cgtaaaacaa tgggtaaacc
    29281 gcccgttgat gacgacattc cggcagaaga acaggtacgt attctgcgta aactgttcga
    29341 tcgctactat ggcgaggttg ccgaagaggg gacgtttttg ttcccgcacg ttgccgatac
    29401 gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg gtcaccaaca aaccgacgcc
    29461 gttcgtcgca ccgctgctcg aagccttaga tattgccaaa tactttagcg tggttatcgg
    29521 cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg ctgttactgg tggctgagcg
    29581 gatgggaatt gccccacaac agatgctgtt tgtcggcgac tcacgcaatg atattcaggc
    29641 ggcaaaagcg gcaggttgcc catcagttgg cttaacctac ggatataact acggcgaggc
    29701 tatcgatctc agccagcctg atgtaattta tcagtctata aatgaccttc tgcccgcatt
    29761 agggcttccg catagcgaaa atcaggaatc gaaaaatgac taagcccatc gtttttagtg
    29821 gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat gggtgcgctg cgtcagtggg
    29881 taaacatgca ggatgactac cattgcattt actgtatcgt tgaccaacac gcgatcaccg
    29941 tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga tacgctggcc ttgtatctgg
    30001 cttgtggtat cgatcctgag aaaagcacca tttttgttca gtcccacgta ccagaacatg
    30061 cgcagttagg ctgggcactg aactgttata cctacttcgg cgaactgagt cgcatgacgc
    30121 agtttaaaga taaatctgcg cgttatgccg agaacatcaa cgctggtctg tttgactatc
    30181 cggtgctgat ggctgcggac atcctgctgt atcaaactaa tctggtaccg gtgggtgaag
    30241 accagaaaca gcacctggaa ctgagtcgcg atatcgccca gcgtttcaac gcgctgtatg
    30301 gcgagatctt taaggtgccg gagccgttta ttccgaaatc tggcgcgcgc gtaatgtcgc
    30361 tgctggagcc gaccaagaag atgtccaagt ctgacgataa ccgcaataac gttatcggcc
    30421 tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg cgcggtcact gactccgacg
    30481 agccgccggt agttcgctac gatgtgcaga acaaagcggg cgtttccaac ctgctggata
    30541 tcctttctgc ggtaacgggc cagagcatcc cagaactgga aaaacagttc gaaggcaaga
    30601 tgtatggtca tctgaaaggt gaagtggctg atgccgtttc cggtatgctg actgaattgc
    30661 aggaacgcta tcaccgtttc cgcaacgatg aagccttcct gcaacaggtg atgaaagacg
    30721 gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa agcggtgtac gaagcgattg
    30781 gttttgtggc gaagccgtaa tttaacatta atgccaaaaa ccggggaaac ccggtttttt
    30841 tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct cgccgtttcc acatctcgtc
    30901 gttgccactt atcttcattt taatgaagat ataaatattc attttattga aaatatatta
    30961 tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac gacgctggcc cgttatttgt
    31021 tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga gctggcaact gaatgccgtt
    31081 catcggttgg cctgacgcag gccgcgttga aaacgttgga atcaagcgga gcgatacgga
    31141 ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat ggataacaaa gcattgctga
    31201 gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt gccctatacc cgcttgtacg
    31261 aaggtttagc gagcgggctg aaagctcagt ttgatggcat tcctttttac tatgcgcata
    31321 tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg cgtgtatgac atggcggtgg
    31381 tttcccgact ggcggcggaa agttatctca cgcaaaaagg gttatgcctc gcgctggagt
    31441 tggggccgca cacctacgtt ggcgagcacc agttgatttg ccgtaaaggc gagtccgcaa
    31501 acgtgaagcg cgtggggctg gataaccgtt cggcggatca gaaaatcatg accgatgtct
    31561 tttttggcga tagtgatgtg gaacgtgtcg atctctctta tcacgagagt ttacaacgca
    31621 ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt ggcggaaaac gaactgacca
    31681 tgctgggatt agaggcgacg ccgctcacag acgatccgcg atttttacag gccaccgaag
    31741 ccgtggtcct gacgcgagtc gatgattacc caatgcaaca actgctgcgt gccgttgtag
    31801 ataaacacgc cctgctcgcc catcaacaac gggtagtgag tggggaacag gaaccgagtt
    31861 attaaacgaa aggcatctga tatggaaacc agactcaacc tgctttgcga ggcaggcgtt
    31921 attgataagg acatctgcaa aggcatgatg caggtcgtca acgtactgga aacagagtgc
    31981 catctgccgg tgcgcagtga gcaaggaacg atggcgatga cacatatggc gagtgcactg
    32041 atgcgcagtc gccgtggtga agaaatagag ccgctggata acgaattgct ggcagagctg
    32101 gcgcaatcca gccactggca agccgttgtg caattgcatc aggtgttgtt gaaggaattc
    32161 gcactggaag ttaacccgtg tgaagaaggc tatttgctgg ccaaccttta tggattatgg
    32221 atggctgcta acgaagaggt ttgaagctcc cgacaaatcc acgtcgcaca acctaaatat
    32281 tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg caacaagatg tttgtagaag
    32341 cattgaaacg ccagaacccg gcgctgattt ccgccgcact aagtttgtgg cagcagggca
    32401 agatcgcccc ggacagctgg gtgatcgacg tggatcaggt actggaaaac ggtaagcgac
    32461 tgattgagac ggcgcggctt tacggcattg aactgtatct gatgaccaag cagtttggcc
    32521 gtaacccatg gctggcggaa aaattgctgg cattaggtta cagcggcatt gtggcggtgg
    32581 attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc ggtggcacat caggggcatc
    32641 tggtacaaat cccttgtcat caggttgctg acgccgttga acagggcacc gacgtcatca
    32701 ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc agcggtgaag gccggacgtg
    32761 tgcagtttgt gctgctcaaa gtttatagcg acgatgattt tctttatccg gggcaggaga
    32821 gcggttttgt acagcattcg ctgcacgagg tggttgctga aattaagaaa ctgccggggc
    32881 tgcatttagc cggacttacc catttccctt gcctgctttg ggatgaggct gccggaaaag
    32941 ttttgccgac accgaatctt cacacgctga tacaggcacg ggatcaactg gcgaaatctg
    33001 gtattgcact tgagcaactg aacgcgcctt cagcgaccag ctgcacttcg ctgccattac
    33061 tggcgtaata cggtgtgact catgccgaac ccggtcatgc gctgacgggc actattccgg
    33121 caaaccagca gggcgatcag tctgaacgta tcgcgatgct ctggttgagt gaaatctccc
    33181 atcatttccg tggcgacagc tactgctacg gcggcggtta ctaccgtcgt ggtcatgcgc
    33241 aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac tgaaaccaat ctcaaaactg
    33301 tggatgacag cagtatcgac tacaccctgt cgctggcagg cgagtttccg gtaagcagtg
    33361 cagtggtgct ctgtttccgc acgcagattt ttgtcacccg tagcgatgtg gtgctggtgt
    33421 ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta tgacagtctt ggaaactctc
    33481 tgggggcgta atggcgcgat ttgtggtgtt agtgattgat agctttggcg taggggcaat
    33541 gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg aatacatgtg gtcacatcct
    33601 gagccagttg ccgcatttgc agctaccaac gctggagaag ctggggctaa tcaacgcatt
    33661 gggttatgcg ccaggcgata tgcagccgtc agattccgca acctggggcg tggcagagct
    33721 gcaacatgaa ggtggcgata cctttatggg gcatcaggaa attttaggca cgcgcccgtt
    33781 accgccgctg cggatgcctt ttcgcgatgt gattgaccgt gttgagcagg cattagtatc
    33841 cgccggttgg caggtggagc gccgtggcga tgaactgtaa tttctgtggg tcaatcaggc
    33901 ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa gtctataaca ttaccgccaa
    33961 tctctctgtg atctcttttg acgacgcaat caaaattggt cgtatcgtgc gtgagcaggt
    34021 acaggtcggt cgggtcatta catttggtgg cctgttaacc gacagtcaac gcattctcga
    34081 tgccgcagaa agcaaagaag ggcgctttat tggtatcaat gcgccgcgtt ctggcgctta
    34141 tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc gataaaaaag tgcaggtgcc
    34201 gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg ggtaaggtgg cagatatcgt
    34261 cagcaatcct tatggcgtga gctggcaaaa cctggtggat agccagcgga ttatggatat
    34321 caccctcgac gaatttaaca cccatccgac ggcgtttatt tgcaccaata tccaggaaac
    34381 cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc gaacgtttgc aggtcgttga
    34441 ccgtaacctt gcccggcttg ttgatgcgat gcagccagat gattgcctgg tcgtgatggc
    34501 ggatcacggc aacgatccga ccattggtca cagccaccat acccgcgaag tggtgccgct
    34561 gctggtttat cagcaaggcc tggctgctac gcagctcggt gtgcgcacca cgctttctga
    34621 tgtgggagct accgtgtgtg aatttttccg tgcgccaccg ccacaaaatg gtcgctcttt
    34681 tctttcctcc ctccggtttg caggagacac cctatgagtt ttgatccgac gggttacacc
    34741 ctggcccatg agcatctgca tattgatctc tccggcttta aaaacaacgt ggactgccgc
    34801 cttgatcagt atgcgttcat ttgccaggag atgaacgacc tgatgacccg gggtgtacgt
    34861 aatgtgattg agatgaccaa ccgttacatg gggcgcaatg cgcaatttat gctcggtgta
    34921 atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt attaccagga tgcgtttttc
    34981 ccggaacatg tggtgacccg cagcgtgcag gaactggcgc aggagatggt cgatgaaatt
    35041 gaacagggta tcgatggcac ggagctgaaa gccgggatca tcgcggagat cggctccagc
    35101 gaaggaaaga ttacgccgct ggaagagaag gtatttattg ccgctgcgct ggcgcataac
    35161 cagaccggac gcccgatctc cacgcatacg tcgttcagca ccatggggct ggaacaactg
    35221 gctctgttgc aagcccacgg agttgatctt tcgcgcgtca ccgttggtca ctgcgatctg
    35281 aaagacaacc tcgacaacat tttgaagatg atcgatctcg gcgcgtacgt gcagttcgac
    35341 accattggta agaacagtta ctacccggac gaaaagcgca ttgcgatgct ccacgcgcta
    35401 cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg atattacgcg ccgctcccat
    35461 ttaaaagcca atggtggtta tggctatgac tatttattga ccacctttat tccgcaattg
    35521 cgccagtcag gattcagtca ggccgatgtg gatgtgatgt tacgtgaaac tccctctcaa
    35581 tttttccaat aaggacagac tcatgaaaaa gattggcgtt gcaggcttac agcgtgagca
    35641 gattaaaaaa accattgaag cgacggctac tggcagtttt gaagttttca ttcacaacga
    35701 catggaagcg gcaatgaaag tgaaatccgg gcaactggat tactacatcg gtgcgtgtaa
    35761 taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg attggctata acaaaagttg
    35821 caccattgcc aaaccaggca ttaaagcgaa agacgagcat atcgccaaaa tgatcgctga
    35881 aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa cacgcgattc cgatgctgat
    35941 taaccatctg aaataaaagg cacgactatg gatctgtata ttcagattat cgtggtggcg
    36001 tgcctgacgg gtatgacatc gcttctggcg catcgctcgg cggctgtttt tcatgacggt
    36061 atccgcccga tcctgccgca actgattgaa ggctatatga accgtcgcga ggcggggagt
    36121 atcgcttttg gtctgagcat tggttttgtg gcctcggtgg ggatctcttt taccctgaaa
    36181 accgggctgc tcaacgcatg gttactcttt cttcctaccg atatcctcgg cgtactggcg
    36241 ataaacagcc tgatggcgtt tggtcttggc gctatctggg gcgtgttgat ccttacttgc
    36301 ctgttgccag taaaccagct gctgaccgcg ctgccggtgg atgtattagg tagcctcggg
    36361 gaattaagct cgccggtggt ttctgctttt gcactgttcc cgctggtggc gattttctac
    36421 cagtttggct ggaagcaaag tctggtcgcc gccgtggttg ttctgatgac ccgtgtggta
    36481 gtcatgcgct atttcccaca tcttaaccct gaatccatcg aaatctttat tggcatggtg
    36541 atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc gtgatgaaaa tgatattgat
    36601 gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga ttatcaaaaa cttaccctat
    36661 atcgccatcg tgggagcatt gattgccgcc gttgccagca tgaagatttt cgccggcagt
    36721 gaagtgtcga tcttcactct ggagaaagcg tactccgcag gcgtaacgcc ggaacaatcg
    36781 caaacgctga tcaaccaagc ggcactggca gaatttatgc gcgggctggg ttttgtaccg
    36841 ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag ttgcgggctt tacctttgtt
    36901 tatgcggtgg gctatctctc gccgaatccg atagttgcag cggtattagg cgcagtggtt
    36961 atttcggcgg aagtcttgct gcttcgttcg atcggcaaat ggctgggacg ctacccgtcg
    37021 gtgcgtaatg cgtcggataa catccgtaac gccatgaata tgctgatgga agtggcgctg
    37081 ctggtcggtt cgattttcgc agcaattaag atggcgggtt ataccggatt ctctatcgcg
    37141 gttgccattt acttcctcaa cgaatccctg ggccgtccgg tacagaaaat ggcggcaccg
    37201 gtcgtggcag taatgatcac cggtattctg ctgaatgttc tttactggct tggcctgttc
    37261 gttccggctt aaggaaggca cctatgaaga cgtttcctct gcaaagtctg acgattattg
    37321 aggcgcagca aaagcagttt gcgctggtgg atagcatttg tcgccatttc cccggcagcg
    37381 agtttcttac tggcggtgat ttaggcttaa cgccaggact gaatcaaccg cgcgttaccc
    37441 agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca ggctgcggcg ctggtgcagg
    37501 gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt gctcaaaccg gggcagcgtc
    37561 ttctggtaca tgatgcgcct gtttacccga cgacacaggt cattattgag cagatggggc
    37621 tgacgcttat tactgctgat ttcaatgacc tgtcggcact gaagcaggtc gtcgacgagc
    37681 aactaccgga tgcggcgctg gtgcagcata cgcgccagca gccgcaggac ggctacattc
    37741 tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc tgcattaacc gatgacaact
    37801 atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg ggcaaatgtc tcgacttttt
    37861 cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt ggtaggcgat gccgatgtta
    37921 ttagtcggat tcgctccacg ctctattccg gcggcagcca gattcagggc gctcaggcgc
    37981 tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca cgcggtgcag gcgggggtat
    38041 ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc ggaagtgaaa agcgcggtga
    38101 ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca tcagccgatt gccgccagag
    38161 tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc ggtgggtgca gagtcgaaat
    38221 atgaaatccc gccgctcttt tatcgccttt ccggaacgtt tcgccaggcg aatccacaat
    38281 cagaacattg tgcgattcgc attaacccga atcgcaacgg tgaagagacg gtgctgcgga
    38341 ttttgcgtga gagtattgcc agtatttaat gctggatagc accgtcaagc taaattccgt
    38401 actgaacggt cccctcgccc ctttggggag agggttaggg tgaggggaac gtacgggatg
    38461 taaaattcaa cgcgccagca tcgccggatg cgatgctggc gcatcttatc cggcctacgg
    38521 ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg gcgcggcaga agagttcgga
    38581 gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg ctgatgcgaa acaacggttc
    38641 gcccagttcg catttcagcc attttgcctg cgggcgggtg gcggcaaaga tatcgatggt
    38701 ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga aataactgat aggtggagct
    38761 tccttcgacg taaatctcat caaagtcagg ataacgcgac agcgggatcc atgaactatc
    38821 aataaacagc ggctctttat cgagatacat cacccggcag agatggaaca cttcgctgtt
    38881 tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg acccgttcct gttcgatcac
    38941 tttctctttc gtcgccttgc cttgtgagac gccaaaatcg gtaaaaccac tgacagtaag
    39001 cagggcgttt tcaactttct ggctttgtac aaaggtgcct ttgccctgcc agcggatcag
    39061 tacgccgtct gccactaagt cgctgatagc tttgcgaata gtaatgcggc tgacgttata
    39121 ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg gcctggtaaa ccccctgcgc
    39181 gatatcatcc agcagccgct ggcggacggt agcgtagagg agttgatgag agtagcggtc
    39241 cgtagctgac ataatcgacc ctgaaataaa gaatgaatta cacggattat agggtcgttt
    39301 tccggcaagt aacagcacgt tggcgcggag atgctcacgc caacgttata cctaccaggc
    39361 accgtgatac tgaatggttt tcgccgcgca cgccgttccc tgcgccatcg cctgcggtaa
    39421 tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg aacgaatctc cggcacccat
    39481 ggtgtcgata accgtcaccg gttcaggagc ctgacgccag aactgcgcgc catcccaggc
    39541 aatgctgccg ttttcaccca gcgtgacaat cactgttcct gcgccacggg caacaatcgc
    39601 tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg gaggcaaagg caaaatcgag
    39661 atgcggcacc agtgtctgcc agagcgggct gtcccacttg tcggagaagt cgaaagcggt
    39721 aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc gcatgtcccc agattgccgc
    39781 gtgcacaatg tcatactgcg ccagccaggc gtaatcctct tcactcaggg caaagtcggc
    39841 catcacgcct tcggtgtagt cgccaaaaac gcgatcattg tcgtgcagtt ccacctgagt
    39901 ttgtgcggta acgccgtgtt tcgtatggac atggctgata tcgacgccca tgcgggcgag
    39961 atcctgcttc agctttgtgc cgtagtcatc gtcacccacc aaggtaatgc atcccggctg
    40021 tatgccgtag cgagtgcagt acaccgccac attgaccgca ttaccgccag aaaacgcttt
    40081 attcagttgc gggtagatat cgacgcagtt atcgccgatt gtcgccaggg ttttcatctc
    40141 tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct ggcgggcata gagtctgggc
    40201 tcgttcatat acatcgtcac cagctccacc gtacagtaac cctcatagcc ccgctcaata
    40261 atatcgcgca tcagttcccg cagcggcatt ttgccttctc caggaatgta atgcgtgtcg
    40321 ctggccccgt cgctgtcgac aatatgcaga tgacgataat ttatcgccca gtttgtcgaa
    40381 ataactcatc accggttccg cctggacata cggcgcgcaa atgtcgacca tgctgaacag
    40441 gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca ttagcattac acacgacgtt
    40501 cgattcatac ggcgttaacg gttcgaggat tagatccatg ccgatgtttt ccgcgtactc
    40561 acacagttcg cttaggttct ctgccagccg tccccagata acattaggtg gcgtgagata
    40621 gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc atctcttttg ccatatccat
    40681 cgccagcttg atcatgtcga ggctttcgcg acgcatatgt tcatcgccca gcatcatgtt
    40741 atacggatag ccgttggttt ctggcgtata gccgataatc ggcatctgat acgtctgcgc
    40801 cagcgccttg atttgtttga tgccgcccgc ttttaagtcc ggcgcgaacg cgtgcgggcg
    40861 accgccccaa atttcgatgc cgtcgtaacc cagctcgctt gcatcacgaa atgcgtgttc
    40921 aataggcagc cgctggtggc cgcaggtaaa catacctgtt ttcatcgggt aactccttgc
    40981 gtaaatccgg cccgttgccg ggccgggaga gggattaata ttccaccaga ccaccgtaat
    41041 agcggcgttc atccgggttg tgatctttgt aaatagacag gtagtagcag agccactcca
    41101 ttggcacgaa catcaggaac ggtgccagcc acgggtgcag cccttgcgaa atttcggcgt
    41161 aatcgatgac gatcacgttg tcagtacgct gtttaacaaa gttaatggcg cgttcggtgg
    41221 tgtggcgact ttcatcattg ccgagcagga acaggaacgg aacgcccggt tcgacaatct
    41281 ccagcgggcc atggcggaac tctccgctct caatcacgca gccgtgcgtc caggtaaatt
    41341 ccatcagcgt tacaatgcct tctttgtaac ccagcggacg cagcggaccc gcagcaacgg
    41401 tataaatcat cggccactgg ctggccagtt caccaagctg gcggcctttt tcttcccagg
    41461 tgcgtaccag atgaccgagc gcattcggca acggcttgag atcgtttttg atcttgccga
    41521 tttccgcgtt cggcgcgagg cgggtgatca tctccagcac cacgctgtag cagagcagca
    41581 ggtgaatttc ccagatacag tcggcctgat aatcaatgct aaattccgcc gccgaggtaa
    41641 tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc gccgcaggcc cggcccagct
    41701 ccagcgcttt gattacctct tccgttttac cgtagtcaga aacgccaatc accgcgcaac
    41761 gatcgtcgag gcgatacggg gtgttatcgc agaactccca gccggaaatg gcgtagacct
    41821 gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg cgccgcgttg agtggcgagc
    41881 cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac catctcttcc acgatggcgt
    41941 gcaccagcgg aacgtcatgg ctgagaactt tttccacttc ctgaaccata ttttcggtga
    42001 ccagaaagtc cacggtgctt ttatcaatat ccaacatttt attttctcca gataacatta
    42061 cgacaaagcg ttgagctggc ggctacgctt cgcccagaag gcgtaagcag gcagaccagt
    42121 agcaataacg atgacggcgc agataaggcc gggaatcggt gcccagacaa aggttgaggc
    42181 gaccagaatg aggcttgacg caatggcgag ggtggtcatc agcccgaaag ccggagtacg
    42241 ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg atggagccga aggtgagggt
    42301 atttttgaaa cacatcacca gggtgaaata acccagcagg ctggtgagat cggaaacgaa
    42361 gatgaagaag atccccagcg ccccttgcag gatgatggag acatccggcg tgttgtattt
    42421 cggatgcaca tggccgaagc atttaaagaa caggttgtct ttcgccatcg cgtattccag
    42481 ccgcggctgg tacatcacgc agctggaaag cgaaccaaga atgacgatca tcgccgtgat
    42541 ggcaacaaag atcccagcgg tgctgccgag tgcggggatc caggtcaggg cgtcggaaat
    42601 cggcgtttca gaattggcga gtttgtcgaa gggcatcagg ccggaaatca ccagcgccag
    42661 cagggtgtag agcaccagaa ccagcagaca ggaaccaatc agcgctcgtg gcatggtttt
    42721 tccggggttt ttaatttcgc cggtcatata acagatagag gccatgccgg tatacgacca
    42781 actggtggca gagatccccg ccagcagcgc cataaagctg cccgttgcgc caatcgcagt
    42841 ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag atcccaaggc caatgacgat
    42901 agtgaacggg ataattttgg cgatggtaat tagcgtctga aacgctgcgc cgccttcaac
    42961 ggagcgcagg tgtagcaaca taaaggcgat aattaatccg gcggcgataa atttaccgag
    43021 caacggatcg ataggcgtta aaaagccaag attgctgaca atcgccagcg ccataatcga
    43081 caatgacggc gcatcgttgg cccagaagct ggcccagccg gagaggaaag ccagcggtcg
    43141 gcttccggca tttttcagat aaacataatc tgcgccattt tccggataag cggtggatag
    43201 ttccgca
//
LOCUS       DAJTVE010000016        40837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000016 DAJTVE010000000
VERSION     DAJTVE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            42..1706
                     /gene="asnB"
                     /locus_tag="OO439_000869"
     CDS             42..1706
                     /gene="asnB"
                     /locus_tag="OO439_000869"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6315310.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2086..2162
                     /locus_tag="OO439_000870"
     tRNA            2086..2162
                     /locus_tag="OO439_000870"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2120..2122,aa:Met,seq:cat)
     gene            2171..2255
                     /locus_tag="OO439_000871"
     tRNA            2171..2255
                     /locus_tag="OO439_000871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2205..2207,aa:Leu,seq:tag)
     gene            2279..2353
                     /locus_tag="OO439_000872"
     tRNA            2279..2353
                     /locus_tag="OO439_000872"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2311..2313,aa:Gln,seq:ttg)
     gene            2388..2462
                     /locus_tag="OO439_000873"
     tRNA            2388..2462
                     /locus_tag="OO439_000873"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2420..2422,aa:Gln,seq:ttg)
     gene            2478..2554
                     /locus_tag="OO439_000874"
     tRNA            2478..2554
                     /locus_tag="OO439_000874"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2512..2514,aa:Met,seq:cat)
     gene            2603..2677
                     /locus_tag="OO439_000875"
     tRNA            2603..2677
                     /locus_tag="OO439_000875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2635..2637,aa:Gln,seq:ctg)
     gene            2714..2788
                     /locus_tag="OO439_000876"
     tRNA            2714..2788
                     /locus_tag="OO439_000876"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2746..2748,aa:Gln,seq:ctg)
     gene            complement(2942..4117)
                     /gene="ubiF"
                     /locus_tag="OO439_000877"
     CDS             complement(2942..4117)
                     /gene="ubiF"
                     /locus_tag="OO439_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6315311.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4263..5687
                     /gene="miaB"
                     /locus_tag="OO439_000878"
     CDS             4263..5687
                     /gene="miaB"
                     /locus_tag="OO439_000878"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6315312.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5840..6880
                     /locus_tag="OO439_000879"
     CDS             5840..6880
                     /locus_tag="OO439_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6315313.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            6877..7344
                     /gene="ybeY"
                     /locus_tag="OO439_000880"
     CDS             6877..7344
                     /gene="ybeY"
                     /locus_tag="OO439_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6315314.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7434..8312
                     /gene="corC"
                     /locus_tag="OO439_000881"
     CDS             7434..8312
                     /gene="corC"
                     /locus_tag="OO439_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6315315.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8337..9875
                     /gene="lnt"
                     /locus_tag="OO439_000882"
     CDS             8337..9875
                     /gene="lnt"
                     /locus_tag="OO439_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6315316.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(9953..11830)
                     /locus_tag="OO439_000883"
     CDS             complement(9953..11830)
                     /locus_tag="OO439_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6315317.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12285..13193
                     /gene="gltI"
                     /locus_tag="OO439_000884"
     CDS             12285..13193
                     /gene="gltI"
                     /locus_tag="OO439_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6315318.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13363..14103
                     /gene="gltJ"
                     /locus_tag="OO439_000885"
     CDS             13363..14103
                     /gene="gltJ"
                     /locus_tag="OO439_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6315319.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14103..14777
                     /gene="gltK"
                     /locus_tag="OO439_000886"
     CDS             14103..14777
                     /gene="gltK"
                     /locus_tag="OO439_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6315320.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14777..15502
                     /gene="gltL"
                     /locus_tag="OO439_000887"
     CDS             14777..15502
                     /gene="gltL"
                     /locus_tag="OO439_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6315321.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15620..16555
                     /gene="rihA"
                     /locus_tag="OO439_000888"
     CDS             15620..16555
                     /gene="rihA"
                     /locus_tag="OO439_000888"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6315322.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16647..18317
                     /gene="hscC"
                     /locus_tag="OO439_000889"
     CDS             16647..18317
                     /gene="hscC"
                     /locus_tag="OO439_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6315323.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18377..19828)
                     /gene="djlC"
                     /locus_tag="OO439_000890"
     CDS             complement(18377..19828)
                     /gene="djlC"
                     /locus_tag="OO439_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6315324.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19825..20532)
                     /locus_tag="OO439_000891"
     CDS             complement(19825..20532)
                     /locus_tag="OO439_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6315325.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20634..21188
                     /locus_tag="OO439_000892"
     CDS             20634..21188
                     /locus_tag="OO439_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6315326.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21198..22625)
                     /locus_tag="OO439_000893"
     CDS             complement(21198..22625)
                     /locus_tag="OO439_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6315327.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22622..23329)
                     /locus_tag="OO439_000894"
     CDS             complement(22622..23329)
                     /locus_tag="OO439_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6315328.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23493..24470
                     /locus_tag="OO439_000895"
     CDS             23493..24470
                     /locus_tag="OO439_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6315329.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24540..25022)
                     /locus_tag="OO439_000896"
     CDS             complement(24540..25022)
                     /locus_tag="OO439_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6315330.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25257..27839
                     /gene="leuS"
                     /locus_tag="OO439_000897"
     CDS             25257..27839
                     /gene="leuS"
                     /locus_tag="OO439_000897"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6315331.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            27854..28435
                     /gene="lptE"
                     /locus_tag="OO439_000898"
     CDS             27854..28435
                     /gene="lptE"
                     /locus_tag="OO439_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6315332.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28435..29466
                     /gene="holA"
                     /locus_tag="OO439_000899"
     CDS             28435..29466
                     /gene="holA"
                     /locus_tag="OO439_000899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6315333.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29468..30109
                     /gene="nadD"
                     /locus_tag="OO439_000900"
     CDS             29468..30109
                     /gene="nadD"
                     /locus_tag="OO439_000900"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6315334.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30133..30744
                     /locus_tag="OO439_000901"
                     /pseudo
     CDS             30133..30744
                     /locus_tag="OO439_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31004..31321
                     /gene="rsfS"
                     /locus_tag="OO439_000902"
                     /gene_synonym="rsfA"
     CDS             31004..31321
                     /gene="rsfS"
                     /locus_tag="OO439_000902"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6315335.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31325..31792
                     /gene="rlmH"
                     /locus_tag="OO439_000903"
     CDS             31325..31792
                     /gene="rlmH"
                     /locus_tag="OO439_000903"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6315336.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31823..33724
                     /gene="mrdA"
                     /locus_tag="OO439_000904"
     CDS             31823..33724
                     /gene="mrdA"
                     /locus_tag="OO439_000904"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6315337.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33727..34839
                     /gene="mrdB"
                     /locus_tag="OO439_000905"
                     /gene_synonym="rodA"
     CDS             33727..34839
                     /gene="mrdB"
                     /locus_tag="OO439_000905"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6315338.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            34850..35938
                     /gene="rlpA"
                     /locus_tag="OO439_000906"
     CDS             34850..35938
                     /gene="rlpA"
                     /locus_tag="OO439_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6315339.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36077..37288
                     /gene="dacA"
                     /locus_tag="OO439_000907"
     CDS             36077..37288
                     /gene="dacA"
                     /locus_tag="OO439_000907"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6315340.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37398..37661
                     /locus_tag="OO439_000908"
     CDS             37398..37661
                     /locus_tag="OO439_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6315341.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37762..38403
                     /gene="lipB"
                     /locus_tag="OO439_000909"
     CDS             37762..38403
                     /gene="lipB"
                     /locus_tag="OO439_000909"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6315342.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38663..39616
                     /locus_tag="OO439_000910"
     CDS             38663..39616
                     /locus_tag="OO439_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6315343.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39825..40790
                     /gene="lipA"
                     /locus_tag="OO439_000911"
     CDS             39825..40790
                     /gene="lipA"
                     /locus_tag="OO439_000911"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6315344.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10244 a        10399 c        10275 g         9919 t
ORIGIN      
        1 aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg
       61 tattcgatat caaaacagac gcagttgagc agcgtaagaa agcactcgag ctgtcacgcc
      121 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg
      181 ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac
      241 aaaaaaccca cgtactggcg gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg
      301 aatatggcga tcgttatcag ttccagacag ggtctgactg tgaagtgatc ctcgcgctgt
      361 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
      421 acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc atcccactgt
      481 atatggggta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa tcgctggtgc
      541 cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagc caggacggcg
      601 aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa gataacgtga
      661 ccgacaaaaa cgagctgcgt caggctctgg aagattccgt taaaagccat ctgatgtctg
      721 atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgctatca
      781 ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggcccc
      841 agttacactc ctttgctgta ggtctgccgg gttcaccgga tcttaaagca gcccaggaag
      901 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
      961 atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact attcgcgctt
     1021 caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt
     1081 ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccataaa gcgcccaacg
     1141 ctaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
     1201 cgcgcgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca
     1261 aaaaattcct tgatgtggcg atgcgcatta acccgcagga taaaatgtgc ggtaacggca
     1321 aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct
     1381 ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
     1441 aagtggcggc gcagcaggtt tctgatcagc aactggaaac tgcccgcttc cgcttcccgt
     1501 acaacacgcc aacctcaaaa gaagcgtatc tgtaccggga gatctttgaa gaactgttcc
     1561 cgcttccgag cgccgctgag tgcgtgccgg gcggcccgtc cgtcgcgtgt tcttccgcta
     1621 aagcgattga gtgggatgag gcgttcaaga aaatggacga tccgtctggt cgtgcggttg
     1681 gcgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct
     1741 gtttcagtta ttgaggtgct tgttgattct gatatgcagt aaggaaatgt tccgggtttg
     1801 ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac
     1861 caccattgtt gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggga
     1921 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
     1981 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
     2041 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aagatggcta cgtagctcag
     2101 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
     2161 catctttttt gcgggagtgg cgaaattggt agacgcacca gatttaggtt ctggcgccgc
     2221 aaggtgtgcg agttcaagtc tcgcctcccg caccattcac cagaaagcgt tgtacggatg
     2281 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
     2341 ggtaccccag ccatcttctt cgagtaagcg gttcaccgac cggttattgg ggtatcgcca
     2401 agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc
     2461 catcgaagaa acaatctggc tacgtagctc agttggttag agcacatcac tcataatgat
     2521 ggggtcacag gttcgaatcc cgtcgtagcc accaaattct gaatgtgtcg aatacgttcg
     2581 gcaaattcaa aaaccaattt gttggggtat cgccaagcgg taaggcaccg gattctgatt
     2641 ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt tattcaagac gcttacatcg
     2701 taagtgcacc agttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc
     2761 cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc gagctttttg
     2821 cttttctgcg ttcattcaat gtcgaatgcg atgttgacac gtcttatcct tcaatgtcgg
     2881 atgcgacgct gccgcgtctt atccgaccta cggttggcac gcatccggca atgttgtaag
     2941 gctacaaccc taacgcatat ttcagcgcct gacgtttcaa cacgccagca cgctctgccg
     3001 ccattaaccc gagattacgc acaaaacgca gcggcggcag attattactg aatccggcat
     3061 aaaacagatc cataccactt tgcataatga agttatccgc catgcgccgc atctggtaac
     3121 gcttgaggac aggataactg gcccacgctt caccgtagct gcgggcattc accagaacat
     3181 caatcagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc agcggatgga
     3241 tggtatgcgc ggcatcgccc accagcgcaa gccccggctg cacatactgc aacgcatgtc
     3301 ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtaac gtaacccaga cgcgacggga
     3361 aatgcttcgc gatttccgcc tggaattgcg ccatattcat attctgcaac tggcgaatac
     3421 gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc agaaacgcac
     3481 gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc tcacactgta
     3541 cgctaatcaa catgcacgac tgcgcatact gccaggcatg aacgccaatt cccgccatct
     3601 gccgcacctg agaatttgcg ccgtcggcac caatcaccag tttcgcgcgg atcgtttcac
     3661 cgcccttcag ttccagctcc tgaagatcat tatctcgatg taatgcaatc agcgagcctg
     3721 gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacagcgcc tgttgcagga
     3781 cggtgttttc caccatatag ccaagcaacg gtagcttaag ttcagcggcg tcaaacacca
     3841 catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa cgcatagcct
     3901 gtactgcatc ccagacccct aaccctttaa gcaatgatac cgatgcggcg ctgatcgccg
     3961 agatccgcac gtccggttgg ctatcagcga caaacggtgc aggttctgcg tgctcgatca
     4021 ccgttaccga aaatccgtgc tgtgccagcc ccagcgccag cgcgccgccg accattcctc
     4081 cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct gttagcaata
     4141 tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac tggtcacaac
     4201 ggcagcaaag cattacacta tacgccctgc attcctggct actatttcgc aagagcaagt
     4261 cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag tacgattcat
     4321 cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac gtggcggaag
     4381 aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag gaaaaagtct
     4441 tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg attatcggcg
     4501 tcggtggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc cactatgtcg
     4561 atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac tccgtgcgcg
     4621 gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt gaccgtctac
     4681 cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc tgcaataaat
     4741 attgcaccta ctgcgtggtg ccttacaccc gtggtgaaga ggtaagccgt ccgtctgacg
     4801 atattctgtt tgagattgcc cagcttgcgg ctcagggcgt gcgtgaagtc aacctgctcg
     4861 gtcagaacgt gaacgcctgg cgtggtgaaa actacgacgg ctccaccgga tcgtttgccg
     4921 atctgctgcg tctggttgct gcgatcgacg ggatcgatcg tattcgcttt accaccagcc
     4981 atccgatcga attcaccgac gatatcatcg aagtgtatcg cgacacgccg gagctggtga
     5041 gcttcctgca tctgccagta caaagcggtt ccgatcgcat tctgaacctg atgggccgta
     5101 ctcacacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg cgtccggata
     5161 ttcagatcag ttctgacttc attgttggct tccctggcga aaccaccgaa gacttcgaga
     5221 aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt atcttctctg
     5281 cacgtccggg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa gagaagaagc
     5341 aacgtctgta tattctgcaa gaacgtatca atcagcaagc gatggcatgg agccgccgga
     5401 tgctcggcac cacccagcgt attctggtag aagggacttc gcgtaagagc atcatggagc
     5461 tttccgggcg taccgaaaat aaccgcgtgg tcaacttcga gggcacaccg gatatgatcg
     5521 gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc ggtaaagtgg
     5581 tgcgtactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg gtgattgccc
     5641 gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc tcaggcccgc
     5701 cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa tgcttgcgcc
     5761 tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct tccggcggca
     5821 tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg agccagcaga
     5881 caacgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaagc agctcgaacg
     5941 ccgtctcggc atcgagatca atcgccgcga caaccacttt aaactgaccg gccgtccgat
     6001 ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatacag ccccgatgcg
     6061 cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag aagcgcgagt
     6121 actggagcaa agtgcggaga gcgtgccgga gtacggcaaa gcggtcaata tcaaaaccaa
     6181 acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca atattctcga
     6241 ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc tggcagtggc
     6301 tgcggcagtt gatgccctgg agcgtcagga gattcgccgt attctgctga ctcgtccggc
     6361 ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga aagtagaccc
     6421 gtatctgcgc ccactgtacg acgcgctgtt tgaaatgctg ggctttgaga aagttgagaa
     6481 actgattgag cgcaacgtta ttgaagtcgc gccgctggcc tatatgcgtg gtcgtacgct
     6541 gaacgacgcg tttatcattc tcgatgagag ccagaacacc accatcgaac agatgaagat
     6601 gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg tcacacagat
     6661 cgacctgccg cgtaatacta aatcaggctt acgtcacgcc atcgaagtgc tggccgatgt
     6721 cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc ctgtggtggc
     6781 gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgaa aagcggcgct
     6841 ggcggcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat cctcgattta
     6901 caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca aacatggctg
     6961 aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt ggtcgatacc
     7021 gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac caacgtgctc
     7081 tccttcccgt ttgaagtgcc gcctggcatg gaaatgtcgc tactgggcga tctggttatc
     7141 tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga ggcgcactgg
     7201 gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat cgaagatgac
     7261 gaagcagaag aaatggaagc cctcgaaaca gagattatgc ttgctctggg ctatgaggat
     7321 ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg tccccgccgt
     7381 tggtgattaa cgacgacgtt ttgattaact aacatgagaa cccatacgac gccatgagcg
     7441 acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc tccctgttac
     7501 tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg ctgatccgtg
     7561 attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa ggggtgatgg
     7621 acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg attaccctga
     7681 aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc cactcacgtt
     7741 tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg aaagacttgc
     7801 tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta cgtcaggcgg
     7861 ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc tctcagcgtt
     7921 accacatggc gatcgtcatt gacgaattcg gtggggtttc cggtctggtg accattgaag
     7981 acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa gatgatatcg
     8041 acttccgtca gctgagtcgt catacctgga ccgtgcgtgc actggcttcc attgaagact
     8101 tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc ggtggtctgg
     8161 tgatgcaggc atttgggcat cttccggcgc gtggcgaaac tatcgacatc gacggttacc
     8221 agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc aaaatcccgg
     8281 atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat aactacatgg
     8341 cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta ttattcggtg
     8401 cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt atttcgttga
     8461 tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct attggctttt
     8521 gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc atcgcgacct
     8581 ttggcggaat gcctggcccg gttaacatct tcctggtggt gctgctggcg gcgtatttgt
     8641 cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa accacctggc
     8701 tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg cgcggttggg
     8761 tactgacagg cttcccgtgg ttacagttcg gctatagcca gattgatggc ccgttaaaag
     8821 ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg gttagtggcc
     8881 tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc gtcgtgctgt
     8941 ttgccctacc cttcccgctg cgttacatcc aatggtttac cccgcaaccg gagaaaacca
     9001 ttcaggtttc gatggttcag ggcgatattc cgcaatcact gaaatgggat ggagatcagc
     9061 ttcttaatac gctgaagatt tactacaacg caacggctcc gctgatgggc aaatcatcgt
     9121 tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa ccgttcctca
     9181 aagcactgga cggtgagttg cgcgataaag gtagctcgct ggtaaccggg attgtcgacg
     9241 cgcgtctcaa taagcagaac cgctacgata cctacaacac catcatcaca ctgggtaaag
     9301 gtgcaccata cagctacgaa tcagccgatc gctataacaa aaaccatcta gtgccgtttg
     9361 gcgagtttgt cccgctggag tcgattctgc gtccgttagc accgttcttt gatctgccga
     9421 tgtcgtcgtt cagccgtggg ccatatatcc agccgccgct gtcggtaaat ggtattgagc
     9481 ttactgcggc tatttgctac gagatcattc tcggcgagca agtgcgcgat aacttccgcc
     9541 cggataccga ctatctgctg actatctcca acgatgcgtg gtttggtaag tctattggtc
     9601 catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc ccactgttgc
     9661 gcagcaccaa caacggcatt acggcggtga ttggcccgca gggggaggtt caggcgatga
     9721 tcccgcagtt cacccgcgag gtgttgacca ctaatgtgac accgaccacc ggactcacac
     9781 cgtatgcacg taccggcaac tggccactgt gggtgctgac ggcgctgttt ggttttgccg
     9841 ctgtgttgat gagtctgcga gcacgtaaac gttgatgtgc ctgatgtatc ctgcatgagc
     9901 aaaacgtact ttctcattga gctttggggc atggttatca tgcccctttt tcttacggtt
     9961 cagggataca agccacattg ccatattggg gatgacgaat aattgcctgc ggatcaagcg
    10021 agcgcgtcct gtccatttgg atcaacccgt tcttcgccat gacaaatatt tttctaccat
    10081 ccgctgaaaa acgaaggtga ttaacgtagc tggcaggata aacgccaata atctgcccgg
    10141 cattaatatt gagcaggatg acataacctt gcaggccata tgacaccaac gctaaatatt
    10201 gaccgtccgg gctaataccc tcaacggaat aaacgctcct gctacaccat tgcgtcataa
    10261 tgctggcttg ttgatcccct acgcttatac gtgaaatccc ctgactatcg gcaaaaataa
    10321 cctcgccgtt ttcaggtcga aaacgcatcg ccgatatcgt tccacgcgca tcacctgcgg
    10381 gaaagtcttc tttctgatgc gtgatggtat tgatccgcac gatgttacct ctgcctgctc
    10441 ccggaaccag taatgttctt tcatccgggg aaagtgccat aacccaggcg cctggtttac
    10501 cgctcgtccc cgtcccgtcg tatttctcca gcgggatatc cgccacatgc tgataagtca
    10561 ccagatcaat cgctggcaca ctattactgg tgcgattcat cacccaggct tttgtgccat
    10621 cacgcgagag gataaggctg tcaggatgtg caccggtggt aatcgactga ataatctccc
    10681 ctgtcgccac gttaatgacc gataacgcat tcgcttcaaa tgaagtcgca taaatcaact
    10741 tttcatccgg gcttaatgcc agcgatctga cgccctgatc tttacagcca ccacaatgtt
    10801 tgactgactt ttctttcgct atgggcgctg taaaggtatg caggattttc ttttgtccga
    10861 ggtcgaaaac aacaatgctg ttcgtgtttt caattgcggc atacaacata ttaccatccc
    10921 gactgatcgc catatcatgg atatcggcaa tctcagcgag caaatcacca ctggcctgtt
    10981 cacgacttac cggagcggat aatgtttccc tgagggcgtt gcgttcctcc gctaactgct
    11041 gctttttttg ccgtcgctct tgttcaatct cctgtggata aaacgcttca cgcaggcttt
    11101 gcctgacctg taaaactgac tcatccatct gctgctgcgc aagccagata gccccggtca
    11161 gaagaagact gcccgcaaca ataatcccgc cttccgtaac gagtcggttt tgcccagata
    11221 aacgcgcgct cagccaaccc aacgcgccac ccgcaatcag gccgccaatg tggcaagcgt
    11281 tatctattcc tgtttgccgg gcaccgtaca acagtgtcag cgccaccata cccaacagag
    11341 gaaatactcg ccgctggttt ttatggtatg tgcccgtacc tgaagcaaac tgtgttgcta
    11401 tcgctgcacc agctattccc agaatcgccc cggatgcccc aacgctgatg actatctgct
    11461 cactttcgcg taacgcataa caggcgctga tgagcccacc aaagacgccg gagaataacc
    11521 aggtaatcag gagtcgccat ttccccagaa tctgttccgc cacgcctccc acgaccagga
    11581 atgcaaacat attcatcagc agatgagaaa cactactgtg cagaaagata ctgctcaata
    11641 agcgccaggg ttcacccgac aaagtaagag gcgcaacgtt tgcgccgaga tggataagtg
    11701 cggattgatc gacctcttca taactcatcc attgtgcgag ataacaaaat atcccaatac
    11761 agagtgcaaa aaggataagg gttattgcgg gtaattgaac atttaacggc ttaaccgaag
    11821 atgcagacat aaggtgtttc ttccttgaaa agacaggtaa aaagcatttt tcccgtgtgc
    11881 agttatcatc ccggcaaacg tcccatgccg aaaagataat tctgtccgaa atatgacttt
    11941 ttcagcctaa aattacaatc gtaattaatt cgcccctaag tattttttgg catgtaagtt
    12001 gctttattta ctgtgtaagc gatgaaatca gccgcgacgc aaccaggttg caccaccaca
    12061 aatcagcggt agcaccaaaa aggtgcggtt taagaatttt gcttcggaat ggtgcggcgc
    12121 atctcgcaaa aacaaacaat aaaacagcaa tatctttaca tttagccaaa ctaaatgtta
    12181 cctaacaggc acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa
    12241 cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac
    12301 ctgccacagc aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg
    12361 ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc
    12421 accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact
    12481 cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact
    12541 tgcaggtaaa actgattccg attacctcac aaaaccgtat tccactgctg caaaacggca
    12601 ctttcgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa caggcggctt
    12661 tctctgatac tattttcgtg gtcggtacgc gcctgctgac caaaaagggc ggcgatatca
    12721 aagattttgc cgacctgaaa gacaaagccg tagtcgtcac ttccggcact acctctgaag
    12781 ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag
    12841 atcacagtga ctctttccgc accctggaaa gcggtcgtgc cgttgccttt atgatggatg
    12901 atgccctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg gaaatcgtcg
    12961 gcaagccgca gtctcaggaa gcctacggtt gcatgttgcg taaagacgat ccgcagttca
    13021 aaaagctgat ggatgacacc accgcgcagg tgcagacctc cggtgaagcg gaaaaatggt
    13081 ttgataaatg gttcaaaaat cccattccgc cgaaaaacct gaacatgaat ttcgaactgt
    13141 cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac taattacaag
    13201 aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag actatacgcc
    13261 tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg
    13321 tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat agactggaac
    13381 tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg ttggatctgg
    13441 agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc
    13501 ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg
    13561 ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac ctggtatctg
    13621 gtgatcccgg agctgttgcc ggagaaaatc ggcatgtggt ttaaggccga gctggatccc
    13681 aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt
    13741 tgcgaacagg tacgcgccgc cattcagtcg ttgccgcgtg ggcaaaaaaa tgccgcgctg
    13801 gcgatgggcc tgacgctacc gcaggcttat cgatacgtac tgctgcctaa tgcttatcgc
    13861 gttatcgtcc cgccgatgac ttccgagatg atgaacctgg tgaaaaactc cgccatcgcc
    13921 tcgactattg gtctggtgga tatggcggcg caggcgggta aattgcttga ttactcagcc
    13981 cacgcctggg agtcgtttac cgctattacg ctggcgtatg tgttgattaa cgcggttatc
    14041 atgctggtaa tgacgctggt tgaacgtaaa gttcgcctgc ctggcaacat ggggggcaaa
    14101 taatgtacga gtttgactgg agttccattg tcccttccct gccatatctg ctcgacgggc
    14161 tggtgatcac gctgaaaatc accgtcacgg cagtcgtgat tggtattttg tggggcacca
    14221 tgctggcggt gatgcgcttg tccagctttg cgcccgtagc gtggtttgcc aaagcctacg
    14281 ttaacgtatt ccgctcgatt cctttagtta tggttttgct gtggttttac ctgatcgtgc
    14341 caggttttct gcaaaacgtg ctgggattat caccaaaaaa tgatatccgt ttaatttcgg
    14401 cgatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc cgtgctggta
    14461 ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc gttgggaatg actcactggc
    14521 agtcgatgaa actgattatt ctgccgcagg cgttccgcgc gatggtgccg cttctgctca
    14581 ctcagggcat cgtactgttc caggatacct cactggtgta tgtgttatct ctggccgatt
    14641 tcttccgtac cgcctcaacc attggtgagc gtgatggtac gcaggttgag atgatcctgt
    14701 ttgccggatt tgtttatttc gttattagtc ttagcgcgtc gttgttggtc agctacttga
    14761 aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg gtcactttca
    14821 ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc
    14881 gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac cagtgcagca
    14941 aggtgaaatc accgtcgatg gtatcgtggt taatgacaag aaaaccgatc tggcaaagct
    15001 gcgttcccgc gtcgggatgg tattccagca tttcgagctg ttccctcatc tgtcgattat
    15061 cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc gataaagcgc ccgctcgtga
    15121 aaaagcattg aaattgctgg agcgcgtggg actttccgca catgccaata aatttccggc
    15181 gcagctttcc ggcggtcagc agcagcgtgt ggcaatcgct cgcgcattgt gtatggatcc
    15241 tattgcgatg ctgttcgacg aaccgacatc ggcgctggat ccggagatga tcaacgaagt
    15301 gctggacgtg atggtggaac tggcgaacga aggaatgacc atgatggtgg tgacccacga
    15361 aatgggcttt gcccgcaaag tggcgaatcg ggtgatcttt atggacgagg gtaaaattgt
    15421 cgaagactcg ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg cgaaagactt
    15481 cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac ccaagtgcgc gccatctcac
    15541 gctttatcgt ttcgcccctc gtatctgtta catcctggcg ttacccttgt cgcaaagaag
    15601 cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc caggtcatga
    15661 cgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca aagcaattac
    15721 gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc gtatgctgac
    15781 cttgcttaat cgcaccgata ttccggtagc aggcggcgcg gtaaaaccgt taatgcgtga
    15841 gttgattatc gcggacaatg tgcacggcga aagcggtctg gatggcccgg cattaccgga
    15901 accgacgttc gcaccgcaaa actgtacggc ggtagagctg atggcgaaaa cgctgcgcga
    15961 aagtgcggaa cctgtcacga ttgtgtctac cggaccgcaa actaacgttg ccttgctgct
    16021 caatagccat ccggaactgc atagcaaaat tgcccgtatc gtgattatgg gcggcgcaat
    16081 ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc cggaagcggc
    16141 agaaattgtc ttccagtcag ggatcccagt ggtgatggcc ggtctggatg ttactcacaa
    16201 agcacaaatc cacgttgaag acaccgagcg tttccgcgcg attggcaacc ctgtttcaac
    16261 cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa aatggggctt
    16321 tgtcggtgca ccgctgcatg acccatgcac catcgcctgg ctgttgaaac ccgagttgtt
    16381 taccactgtt gagcgctggg ttggcgtgga aacacagggg aaatatagcc agggtatgac
    16441 ggttgttgat tattattatc tgactggcaa taaaccgaat gccactgtaa tggtcgatgt
    16501 tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg cttaacacac
    16561 gtcattgatt aagatcacca cgcgttacct gggtggtctt aatctgttta tctgtttatc
    16621 tctttttctt tcaagatata aatgcaatgg ataatgcaga actcgccatt ggtatcgatc
    16681 tcggcactac caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa
    16741 ataagttcgg tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt
    16801 tagtcggaaa accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat
    16861 ttaaacgtgc aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc
    16921 cagaactgtc ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac
    16981 gtccgattaa agatgtggtg atctccgttc cggcttattt cagcgatgaa caacgcaagc
    17041 atacccgttt agcagcggag ttagccgggt taaatgcggt acgcttaatt aatgaaccca
    17101 cagcagctgc gatggcatat ggcctgcata cgcaacaaaa tacccgctcg ctggtttttg
    17161 atctcggtgg cggcacgttt gacgtcacgg tgctcgaata cgccacgccg gtgattgaag
    17221 ttcacgcctc cgctggcgat aacttcctcg gtggcgaaga ttttacccat atgttggtcg
    17281 atgacgtttt aaagcgcgcg gatgtcgcca ggaccacgct taacgagagt gaactggcag
    17341 ccttgtacgc ctgtgtggaa gcggcaaaat gtagcaatca atcgccattg cacattcgct
    17401 ggcagtacca ggatgaaacg cgggagtgcg aattttacga gaacgaactg gaagatttgt
    17461 ggttgccgct gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcacgtc
    17521 tgaagccgag tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg
    17581 tgcagcgaat cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gacccgagca
    17641 ccattgtcgc gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg
    17701 aagaggtgat cctcactgat atttgccctt actcgttggg cgttgaagtt aaccgccagg
    17761 gcgtttccgg cattttctcg ccaattattg aacgaaacac cacagtaccc gtgtcgcgtg
    17821 tagaaactta ctcgaccatg cacccggaac aggattctat tacggttaac gtctatcagg
    17881 gagaaaacca caaagttaaa aataacattc tggttgaatc cttcgatgtg ccgttgaaga
    17941 aaaccggggc ttatcagtcg attgatattc gctttagtta tgatatcaac ggcttgcttg
    18001 aagttgacgt gcttctggaa gatggcagcg ttaagtccag agtcattaat catagcccgg
    18061 taacattgag cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt
    18121 atccgcgcga tatgctaatc aaccgcacct ttaaggccaa actggaagag ttgtgggcgc
    18181 gggcgctggg tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgt
    18241 tgcagtcaaa cgatatggcc cgcgtcgatg aagttcgtcg acgggcgagc gattatttgg
    18301 ctattgagat cccttaaaca cggctgccgg atgcggcata aacgctgcat ccggcatcgg
    18361 cgcaaaaaac acatcctcag ctaaacaaca tcgcgatttt tccgagcgca taacctatca
    18421 tcagcaccgt taagctgatt ttgataatac tggtaatgct ctgtggtttt tcatcctcat
    18481 catttttttc ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga
    18541 gtttcacttt cgtcaacagc atctgggtga taatggcctc taatggatga gcatctccac
    18601 cttccagcct tgtattaaga cgggccagca tctcttgttt cgggaacgct tccagcacaa
    18661 cctgttccgc tacccgatac aaatccagta acggtgaatc ttgctctgtc tcgctaccga
    18721 ctctcgattt ccaccacgcc catgctttgc gcttgaagaa taacgactca ataaacgatt
    18781 gacctaacgg cgataatttt ccgctatgta aaatctccgc ccataacagc cgcgtccggg
    18841 cgctctcacc aggaacatat ttcaacggtt taccttgcgc atctaccatg cttcgcgctt
    18901 cgataaccat cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca
    18961 acaaattatc agcttgtgta gatgggtaac tttcccagta cgcgcagaga tcggcaagca
    19021 ggctttcccc ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc
    19081 tttctggttc ttccgcttgc cactgtttgg ctacaggaat gagttccgcc atacctgttt
    19141 gcaccgagct gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc
    19201 acggtccttc catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt
    19261 ccagcgcgaa gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc
    19321 taaggaaatc gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct
    19381 catccattcc ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag
    19441 cacaatttaa tgaaagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt
    19501 gatcgacatc ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg
    19561 gctgaaaacg atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt
    19621 cagcggtggc atctttttct tcatcatcgg cttcctcgac aggatttacc gccagtcgca
    19681 gcgcctcttc ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac
    19741 acaacggtaa gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa
    19801 tttcgaggat ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca
    19861 ttcaggctgg acagttaatg gttgccaggg aagcgtggca tagaaacggt cgcttcccgc
    19921 tgccagctct tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc
    19981 gcgtagcgaa gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata
    20041 ggcagaaaaa tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga
    20101 ttgcagccat aaactctctt cttccgtcag ccagttgttg agcacaccga cccgtgacag
    20161 aaaccccatt cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt
    20221 ttgggcaacg aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt
    20281 cctctcttca ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg
    20341 accatcatca gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca
    20401 ggatgatgag agatcgaatt cttttatccc agtatacatt tttggcgagt catgcgcggg
    20461 tttgcaactc tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc
    20521 ttccttattc attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg
    20581 atgagtataa acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata
    20641 aaaaatcaat gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac
    20701 ccaagaaaac gacgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat
    20761 caccaggcag tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact
    20821 caacatcgca atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg
    20881 attatacaca ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac
    20941 aaagcaaact ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag
    21001 caattctctg gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg
    21061 gtttgatcta cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt
    21121 taaaactggc cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg
    21181 cacattaacg tcgactgtca cccgaagaga tggagtattt ttcctatcaa accggcaaag
    21241 ataaaaatgt agaaaataat tttgatgacg cccagacagc ccagcccgcc atcgtcaacg
    21301 ggcattggct gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc
    21361 tgaattaata atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg
    21421 cggctcacaa tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg
    21481 gcaactaaat ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc
    21541 ttacgtcctt tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca
    21601 tcgctgagta tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt
    21661 agctgtttac cctgatcgtt aaccagttca cgagcttcaa tcatcatcac cactaacggg
    21721 taataatcga cccgatgctg acggcaccat tgcaatatta aagcatccgc ctgggtggag
    21781 ggatactcgc gccagtagtc acaaagttct gccagcaagc tttcgccttc tccacaatag
    21841 acgcgttgcg cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac
    21901 gaccgggcgt aatccagaag ttcggggata tccaggcgac ctgcgctata ccagcggagt
    21961 aaatttctgt gcagttttgc atcatcgggg atgatcactg cgcgatgttg cgctaaatac
    22021 aatgtcagcc attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg
    22081 gtgtcatttt gcacggcaac gggcagatgc agaatggtct ggaagttaaa aacattcccc
    22141 ttcgcaatgg catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc
    22201 tcctgcaacc accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca
    22261 gtgttcatgg cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa
    22321 taatttaatt gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag
    22381 gcaagtaacg cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt
    22441 tgccaaacag atttagcagg cgactgcgca atccggagcg cctcctcata cgcctgacga
    22501 agttgtttaa aaccctgcgg atcggtttct ggatgaaagg acagtaacag cgccagataa
    22561 gcgcggcgga taatatcgac gtcagtcgtt tcctctattt cgaggatctt ccagcaattt
    22621 ttcataggtc gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt
    22681 gccaggacaa ggcagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat
    22741 tgccggaatc gtcgtattct ttacgagcta agaattcgcg taataaaggc agatgatcat
    22801 cttcgggaga ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt
    22861 tactataata acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt
    22921 cactcaacca gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc
    22981 atgctttaat tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag
    23041 cctgaccttg ctcatttagt tcagcaagat agtcacgcca ttcacatggc gaataacgaa
    23101 accagcggtg ataaaaccct gccaaccgag cggcatgacc gttatcgatc attcggtgaa
    23161 ttgtgcgcag caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc
    23221 atgtacctaa atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt
    23281 ccatcggagt ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc
    23341 ttaaacattc atttaatgaa tgtaaactac atttcgagac tcagttattt atacttttct
    23401 gacgcaacat aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa
    23461 tatgttttat ctacaaccat aaggaaatga taatggtttt tacgtcaagt tgctgcgata
    23521 atttatcaat agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata
    23581 ttgttgggtt ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct
    23641 tttactggct gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc
    23701 aaaagtatac cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga
    23761 aaaaagctgc cctacaaggc catactttcg cttccaacgc ccttggctgg acactggatc
    23821 gtggagaagc ccccaattat aaagaagcgg ttgtctggta tcagatagcc gcggagagcg
    23881 gaatgtctta tgcgcaaaat aatcttgggt ggatgtacag aaatggcaac ggagtcgcaa
    23941 aagactatgc gctggcattt ttctggtaca aacaagctgc attacaaggc catagtgacg
    24001 cgcaaaacaa tctggccgat ctttatgaag acggaaaagg cgttgctcaa aacaagacac
    24061 tcgccgcatt ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa
    24121 ttgcatggga ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact
    24181 ggtatctgaa ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt
    24241 ataaacacgg acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag
    24301 ccgctgaatg caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag
    24361 aaggaagacc tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg
    24421 gaaccagaga tgtcagtcag gaaattcgtg agactgaaga tttactgtag agataaaaac
    24481 aagggccggg gaatttcatt cggcccatat tctctttatt gctgtgcgaa atcagactat
    24541 tacggctcaa acgggcgtct ctggaactgg tcatgaccac atttcgggca tagcgtcagc
    24601 acttccggtg tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc
    24661 ccgaccactt ctccgctgtg ataaacccca tgatgattga ggtcctggaa aacttcgcgc
    24721 cattcaagct gcgttttatc ggtgatatct gccagctcct gccataagct ttctttaatc
    24781 acccgcataa agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac
    24841 tcttccaggt cacgtcttac agctcgcgtc agctcatcga cctcggttcg cgttaactcc
    24901 cctgttttta ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca
    24961 ttgcgcaggc gttcgctcag tgacgcgacc agttcacggt aatattgagc aaccttgttc
    25021 atcgtttcat ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct
    25081 ttgctaatac gcccacaaac tctgtaaatt aatgtatgaa taatccgggc atcatttcaa
    25141 cgtcgagtgc gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga
    25201 aaaaccacct caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc
    25261 aagagcaata ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc
    25321 gcacatttga agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc
    25381 cctatccttc tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga
    25441 tcgcccgcta ccagcgtatg ctgggcaaaa acgtcctgca gccgatcggc tgggacgcgt
    25501 ttggtctgcc tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt
    25561 acgacaacat cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga
    25621 gccgcgagct ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg
    25681 agctgtataa aaaaggcctg gtatataaga agacttctgc ggtcaactgg tgcccgaacg
    25741 accagaccgt actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca
    25801 aagttgaacg taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc
    25861 tgctcaacga tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta
    25921 actggatcgg tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca
    25981 tgctgaccgt ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag
    26041 ctgcgggtca tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta
    26101 ttgacgaatg ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag
    26161 gcgtcgatac tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg
    26221 cagcaaactt cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg
    26281 accagcgcga ctacgagttt gcctctaaat acggcctgaa catcaaacca gttatcctgg
    26341 ctgctgacgg ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt
    26401 tcaactctgg cgaattcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata
    26461 aactgactgc gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg
    26521 tttctcgtca gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg
    26581 taatgccgac cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg
    26641 gcattaccag cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc
    26701 cagcgctgcg tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc
    26761 gctacacttg cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc
    26821 tgccggtgga tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc
    26881 gcttcttcca caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac
    26941 agttgctgtg tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg
    27001 aacgtaactg ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg
    27061 tgaaagcgaa agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca
    27121 agtcgaagaa caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg
    27181 ttcgtctgtt tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg
    27241 gtgtggaagg ggctaaccgc ttcctgaaac gtgtctggaa actggtttac gagcacacag
    27301 caaaaggtga tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc
    27361 gtcgcgatgt gcataaaacg atcgctaaag tgaccgatga tatcggccgt cgtcagacct
    27421 tcaacaccgc aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccaaccg
    27481 atggcgagca ggatcgcgct ctgatgcagg aagcgctgct ggccgttgtc cgtatgctta
    27541 acccgttcac cccgcacatc tgcttcacgc tgtggcagga actgaaaggc gaaggcgata
    27601 tcgacaacgc gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg
    27661 tcgtggtgca ggttaacggt aaagtccgtg ccaaaatcac cgttctggtg gacgcaacgg
    27721 aagaacaggt tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg
    27781 ttaccgtgcg taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag
    27841 cgcgggagga agcgtgcgat atctggcaac attgttgtta tctctggcgg tgttaatcac
    27901 cgccgggtgt ggctggcatc tgcgtgatac cacgcaggtt ccttccacta tgaaggtcat
    27961 gatcctggac tcaggcgatc cgaacgggcc attaagccgt gcagtgcgta accagttacg
    28021 tctgaatggt gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg
    28081 tttgggtaaa gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc
    28141 agagtatcag atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta
    28201 cccgattagc gccaaagtct tccgttcgtt ctttgataac ccgcaaatgg cgttagcgaa
    28261 agataacgaa caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat
    28321 tcgtaagctg ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac
    28381 cacaacggat actccggcaa cgcctgcacg cgtctccacc acgctgggta actgatgatt
    28441 cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt
    28501 ttacttggta acgatcctct gttattgcag gaaagccagg acgctgttcg tcaggtagct
    28561 gcggcacaag gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat
    28621 gcgatctttt cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg
    28681 ttgttaccag aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga
    28741 cttctgcatg acgacctgct gttgatcgtc cgcggtaata aattaagcaa agcgcaagaa
    28801 aatgccgcct ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg
    28861 gagcaggctc agcttccccg ctgggttact gcgcgcgcaa aacagctcaa cttagaactg
    28921 gatgacgcgg caaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct
    28981 caggcactgg agcgtttatc gctgctctgg ccagacggca aattgacatt accgcgcgtt
    29041 gaacaggcgg tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg
    29101 atgggaaaaa gtaagcgcgc attgcatatt cttcagcaac tgcgtctgga aggcagcgaa
    29161 ccggttattt tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc
    29221 cagtctgccc atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc
    29281 cggggcatga tgggcgaggc gttaaatcgc ttaagtcaga cgcagttacg tcaggccgtg
    29341 caactcctga cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca
    29401 gagctgaaag ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac
    29461 ggttgatatg aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg
    29521 tcatctaaaa cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat
    29581 ccctaataat gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca
    29641 catgcttgaa ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa
    29701 gcgcaatgcc ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc
    29761 ggacgtgccg ctggcgttta ttattggtca ggattcactg ctgacctttc cgacctggta
    29821 cgaatacgaa acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc
    29881 acttgaaatg gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc
    29941 ggaagatctt caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa
    30001 catctcggcg accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt
    30061 gccggaaccg gtactgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt
    30121 acggagggca tgatgcgact gtggttaatt cgtcatggtg aaacgcaagc gaatatcgat
    30181 ggtctttaca gcggtcatgc gcccaccccc ctgaccgcgc gcggtattga gcaagcgcaa
    30241 aatctgcata cgctgctaca tggtgtttcc tttgatctgg ttttatgcag tgaactggaa
    30301 cgggcacagc ataccgcacg actggttctc agtgaccgcc agctccccgt gcaaatcatc
    30361 cctgaactca acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg
    30421 caagaagatg ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc aatccccacg
    30481 aacggtgaag gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt
    30541 gaatttcagc actatcagaa tattttagtc gtcagccatc agggtgtact gagtctgtta
    30601 atcgcccgtt taattggcat gcctgccgag gctatgtggc attttcgcgt tgactagggg
    30661 tgttggagcg ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt
    30721 gccatcgggg tcgagaatgc atgacttttc tgttttttta cgggtaagcc gcaacgacca
    30781 ttgacagacc cgggcaggct gatattctcc gcagccagac ttttttcgcc agacacgact
    30841 ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc
    30901 ccactttcga aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac
    30961 tgacagacca tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact
    31021 ccaggatttt gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga
    31081 cgttcaaggc aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg
    31141 tcatgttatg tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc
    31201 gctcggcgta gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat
    31261 tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta
    31321 atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg
    31381 ttttaccgag tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc
    31441 ggccggaaaa cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca
    31501 gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg
    31561 ggatacgccg cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtcag
    31621 tctactgatt ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg cggagcagag
    31681 ctggtcgctg tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag
    31741 tctgtaccgg gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag
    31801 ctttgagtag aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga
    31861 gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg
    31921 cgtgcttatc gccaacctgt ataatctgca aattgttcgc tttaccgact accagactcg
    31981 ctctaatgaa aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga
    32041 ccgtaatggt atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccaga
    32101 gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc agcgtggttg atctgaccga
    32161 tgacgatatt gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc
    32221 ggtgaaaact aacctgaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt
    32281 tccgggtgtc gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac
    32341 ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga
    32401 cggcaaactg gccaactatg cggcaacgca tgatatcggt aagctgggca ttgagcgtta
    32461 ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg
    32521 gcgtgttatc cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac
    32581 gctggatctc aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt
    32641 ggttgtcacc gatccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga
    32701 cccaaacctg tttgttgacg gtatctccag caaagattat tccgccttgt tgaacgcccc
    32761 gaatacaccg ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa
    32821 accctatgtg gcggtttcgg cattgagcgc cggggtgatc acgcgcaata cgacgctgtt
    32881 tgacccaggc tggtggcaac tgccaggttc ggaaaaacgt tatcgtgact ggaaaaaatg
    32941 gggccacggg cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta
    33001 tcaggtggcc tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta
    33061 tggtcattac accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga
    33121 atggaaacag aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat
    33181 cggtcagggt tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat
    33241 taatgacggt atcgtgaagg ttcctcattt gctgatgagc accgccgaag acggcaaaca
    33301 ggtgccatgg gtacagccgc atgaaccgcc cgtcggcgat attcattccg gttactggga
    33361 gctggcgaaa gacggtatgt acggtgttgc taaccgccct aacggtacgg cgcataaata
    33421 ctttgccagc gcaccgtaca aaattgcggc gaaatccggt accgctcagg tcttcggtct
    33481 gaaagcgaac gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact
    33541 gatgaccgcc tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa
    33601 cggtggtgcg ggtccggcgg ttggtacact gatgcgccag atcctcgacc acattatgct
    33661 gggtgataac aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca
    33721 ttaatcatga cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc
    33781 acaatgctgc tgatcttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc
    33841 agcggtcagg atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctagtc
    33901 atcatggtgg tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc
    33961 tatatcatct gtattatttt gctggtggcg gtagatgctt tcggtgccat ctctaaaggt
    34021 gctcaacgct ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata
    34081 gccgtaccac tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag
    34141 aacactggca tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct
    34201 gacctgggaa catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc
    34261 cttagctggc gtctgattgg cgtcgcagta gtgctggtgg cggcgttcat tccgattctg
    34321 tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct agacccggaa
    34381 tcagacccac tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc
    34441 ggattacgcg gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa
    34501 cgccataccg actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg
    34561 attctgctcg ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg
    34621 caaaccacct ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc
    34681 ttcgtaaata ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg
    34741 gtcagttatg gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca
    34801 atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca
    34861 gtggctcggg atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca
    34921 gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag
    34981 cggggcggac ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga
    35041 cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc
    35101 aatctatgat gccgaacccg gcagtaacct gacggcctct ggcgaagctt tcgatccgac
    35161 acagctgacg gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct
    35221 ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgacag
    35281 cgttatttca ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt
    35341 tcgtatcgat ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg
    35401 taccacagtc gccaaacaga cttacgccct gccagcacct cccgatttaa gcggtggcgc
    35461 gggaacaagt tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct
    35521 aaaaagcgaa gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac
    35581 gaccttagcg cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt
    35641 tacagctccg tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc
    35701 gcaaagcgcc agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc
    35761 gcaacagtac caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa
    35821 tggcgcggtc tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt
    35881 gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg
    35941 taaagcaggc atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag
    36001 tcagatgcct gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga
    36061 tgtcgtagtt cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct
    36121 caccacggct ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa
    36181 aactatgatc ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa
    36241 ctccggcaaa gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac
    36301 caaaatgatg accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac
    36361 tgatttagtc actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc
    36421 ttcgctgatg ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat
    36481 taacctgcaa tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca
    36541 ggacgctttt gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca
    36601 cttccagacg gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc
    36661 gctgatcggc caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa
    36721 agaatttacg tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag
    36781 cctgaatgtc gacggcatca aaaccggaca cactgacaaa gcaggttaca accttgttgc
    36841 ttctgcgact gaaggccaga tgcgcttgat ttctgcggtg atgggcggac gtacttttaa
    36901 aggccgtgaa gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt
    36961 taacccactg aaagtaggta aagagttcgc ctctgaaccg gtttggtttg gtgattctga
    37021 tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc attccacgtg gtcgcatgaa
    37081 agatctgaaa gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa
    37141 tcaggtcgtc ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct
    37201 ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa
    37261 attaatgttc catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc
    37321 ccatatacta agcatcagta aaaaactccc gccttctggc gggagttgct atttaattac
    37381 gttacgccgg agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt
    37441 ttacttacaa agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg
    37501 tacagcgcca tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact
    37561 accactcggt atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg
    37621 aagaactagg caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg
    37681 gcccctggtc gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc
    37741 attctccacg gagatgcagt tttgtatcag gataaaattc ttgtccgcca gctcggtctt
    37801 cagccttacg agccaatctc ccaggctatg catgaattca ccgatacccg cgatgatagt
    37861 acccttgatg aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga
    37921 aaagcggagc acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg
    37981 caggtgactt atcacgggcc ggggcaacag gtgatgtatg tgttgcttaa cctgaaacgc
    38041 cgtaaactcg gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg
    38101 gctgaactgg gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa
    38161 aagaaaattt gctcactggg tttacgtatt cgacgcggtt gttcattcca cggtctggca
    38221 ttaaacgtca atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga
    38281 atggaaatgg ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgcacca
    38341 cgtttactgg aaaatatttt agcgctacta aacaatcctg acttcgaata tattaccgct
    38401 taattgcata cctcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg
    38461 ctttacatct ccttcgcgtt tgtatacttt ctttatcact tgcagaaatg cggtgctacg
    38521 ccttgctccg taactcaccc cattgattaa cctattgaaa tagttgatta tggtgagcaa
    38581 aagcgttccg ttccaattag acataagatt attgtaattt ttttaaatat tatttttcca
    38641 tgaataaaat tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag
    38701 aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct
    38761 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
    38821 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
    38881 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
    38941 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
    39001 tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc
    39061 tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
    39121 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt
    39181 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
    39241 ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
    39301 gcatgtactc ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga
    39361 gatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact
    39421 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg
    39481 ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
    39541 tatccactac aacaaattca gcctgcgtga tcccatcctg cacggggtaa tagatgttat
    39601 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
    39661 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
    39721 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
    39781 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
    39841 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
    39901 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
    39961 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
    40021 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
    40081 cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg
    40141 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
    40201 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
    40261 cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
    40321 aaactctggt gccggatttc cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa
    40381 cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cgtcaggtac
    40441 ggcctggtgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
    40501 cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat gaagaaatta
    40561 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
    40621 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
    40681 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
    40741 gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata
    40801 attacttaca tctgtctgat aaaaaacccg ctttttg
//
LOCUS       DAJTVE010000017        40756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000017 DAJTVE010000000
VERSION     DAJTVE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..101)
                     /locus_tag="OO439_000912"
     tRNA            complement(25..101)
                     /locus_tag="OO439_000912"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Met,seq:cat)
     gene            310..1407
                     /gene="mltA"
                     /locus_tag="OO439_000913"
     CDS             310..1407
                     /gene="mltA"
                     /locus_tag="OO439_000913"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6315345.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1484..2290
                     /gene="tcdA"
                     /locus_tag="OO439_000914"
     CDS             1484..2290
                     /gene="tcdA"
                     /locus_tag="OO439_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6315346.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2341..2784)
                     /gene="csdE"
                     /locus_tag="OO439_000915"
     CDS             complement(2341..2784)
                     /gene="csdE"
                     /locus_tag="OO439_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6315347.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2784..3989)
                     /gene="csdA"
                     /locus_tag="OO439_000916"
     CDS             complement(2784..3989)
                     /gene="csdA"
                     /locus_tag="OO439_000916"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6315348.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4181..4408
                     /locus_tag="OO439_000917"
     CDS             4181..4408
                     /locus_tag="OO439_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6315349.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4759..5676
                     /gene="gcvA"
                     /locus_tag="OO439_000918"
     CDS             4759..5676
                     /gene="gcvA"
                     /locus_tag="OO439_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6315350.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5695..6090
                     /locus_tag="OO439_000919"
     CDS             5695..6090
                     /locus_tag="OO439_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6315351.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6083..7183
                     /gene="rlmM"
                     /locus_tag="OO439_000920"
     CDS             6083..7183
                     /gene="rlmM"
                     /locus_tag="OO439_000920"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6315352.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7227..7958)
                     /gene="fucR"
                     /locus_tag="OO439_000921"
     CDS             complement(7227..7958)
                     /gene="fucR"
                     /locus_tag="OO439_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6315353.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8016..8438)
                     /gene="fucU"
                     /locus_tag="OO439_000922"
     CDS             complement(8016..8438)
                     /gene="fucU"
                     /locus_tag="OO439_000922"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6315354.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8440..9858)
                     /gene="fucK"
                     /locus_tag="OO439_000923"
     CDS             complement(8440..9858)
                     /gene="fucK"
                     /locus_tag="OO439_000923"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6315355.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9967..11742)
                     /gene="fucI"
                     /locus_tag="OO439_000924"
                     /pseudo
     CDS             complement(9967..11742)
                     /gene="fucI"
                     /locus_tag="OO439_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11775..13091)
                     /gene="fucP"
                     /locus_tag="OO439_000925"
     CDS             complement(11775..13091)
                     /gene="fucP"
                     /locus_tag="OO439_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6315356.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13638..14285
                     /gene="fucA"
                     /locus_tag="OO439_000926"
     CDS             13638..14285
                     /gene="fucA"
                     /locus_tag="OO439_000926"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6315357.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14313..15461
                     /gene="fucO"
                     /locus_tag="OO439_000927"
     CDS             14313..15461
                     /gene="fucO"
                     /locus_tag="OO439_000927"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6315358.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15566..16321)
                     /gene="xni"
                     /locus_tag="OO439_000928"
     CDS             complement(15566..16321)
                     /gene="xni"
                     /locus_tag="OO439_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6315359.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16433..17800)
                     /gene="sdaB"
                     /locus_tag="OO439_000929"
     CDS             complement(16433..17800)
                     /gene="sdaB"
                     /locus_tag="OO439_000929"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6315360.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17858..19147)
                     /gene="sdaC"
                     /locus_tag="OO439_000930"
     CDS             complement(17858..19147)
                     /gene="sdaC"
                     /locus_tag="OO439_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6315361.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19703..21067)
                     /gene="ppnN"
                     /locus_tag="OO439_000931"
                     /gene_synonym="ygdH"
     CDS             complement(19703..21067)
                     /gene="ppnN"
                     /locus_tag="OO439_000931"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6315362.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21179..22027)
                     /gene="queF"
                     /locus_tag="OO439_000932"
     CDS             complement(21179..22027)
                     /gene="queF"
                     /locus_tag="OO439_000932"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6315363.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22095..22640
                     /gene="syd"
                     /locus_tag="OO439_000933"
     CDS             22095..22640
                     /gene="syd"
                     /locus_tag="OO439_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6315364.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23262..23591
                     /locus_tag="OO439_000934"
     CDS             23262..23591
                     /locus_tag="OO439_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6315365.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23591..24373
                     /gene="truC"
                     /locus_tag="OO439_000935"
     CDS             23591..24373
                     /gene="truC"
                     /locus_tag="OO439_000935"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6315366.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24391..24840
                     /locus_tag="OO439_000936"
     CDS             24391..24840
                     /locus_tag="OO439_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6315367.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25275..26627
                     /gene="gudP"
                     /locus_tag="OO439_000937"
     CDS             25275..26627
                     /gene="gudP"
                     /locus_tag="OO439_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6315368.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26629..27969
                     /locus_tag="OO439_000938"
     CDS             26629..27969
                     /locus_tag="OO439_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6315369.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27990..29330
                     /gene="gudD"
                     /locus_tag="OO439_000939"
     CDS             27990..29330
                     /gene="gudD"
                     /locus_tag="OO439_000939"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6315370.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29562..32318)
                     /gene="barA"
                     /locus_tag="OO439_000940"
     CDS             complement(29562..32318)
                     /gene="barA"
                     /locus_tag="OO439_000940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6315371.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32375..33676
                     /gene="rlmD"
                     /locus_tag="OO439_000941"
     CDS             32375..33676
                     /gene="rlmD"
                     /locus_tag="OO439_000941"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6315372.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33724..35958
                     /locus_tag="OO439_000942"
     CDS             33724..35958
                     /locus_tag="OO439_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6315373.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36036..36284
                     /gene="mazE"
                     /locus_tag="OO439_000943"
     CDS             36036..36284
                     /gene="mazE"
                     /locus_tag="OO439_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6315374.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36284..36619
                     /gene="mazF"
                     /locus_tag="OO439_000944"
     CDS             36284..36619
                     /gene="mazF"
                     /locus_tag="OO439_000944"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6315375.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36690..37481
                     /gene="mazG"
                     /locus_tag="OO439_000945"
     CDS             36690..37481
                     /gene="mazG"
                     /locus_tag="OO439_000945"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6315376.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37709..39346
                     /gene="pyrG"
                     /locus_tag="OO439_000946"
     CDS             37709..39346
                     /gene="pyrG"
                     /locus_tag="OO439_000946"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6315377.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39434..40732
                     /gene="eno"
                     /locus_tag="OO439_000947"
     CDS             39434..40732
                     /gene="eno"
                     /locus_tag="OO439_000947"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6315378.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10090 a         9865 c        10840 g         9961 t
ORIGIN      
        1 tgctttactt catcaaattt taattggttg cgggggccgg atttgaaccg acgaccttcg
       61 ggttatgagc ccgacgagct accaggctgc tccaccccgc ctccgtggat gcgcactata
      121 ctccgctcgc gttttcatgc aacctttttt ttaccccgat cacggatctg gcatgatttt
      181 gaacaaaaac aaatcactaa agcctatttt ttgtgcaaaa tttgcgtcag ggagaggctg
      241 ctcatttcat taaacgcggc ggtttgttat cttcgttgcg ccttattttt taacctgaag
      301 aagagaacaa tgaaaggacg ttgggtaaag taccttctta tgggcacggt tgtggcaatg
      361 cttgccgcct gctcttccaa accaaccgat cgcggacagc aatataaaga cgggaaattt
      421 acccagcctt tctctctggt gaaccagcca gatgccgttg gcgcgccgat taacgccggt
      481 gattttgccg agcaaattaa ccatatccgt aattcgtcac cgcgtctgta tggcaaccag
      541 agtaatgttt ataacgcggt gcaagagtgg ctgcgcgcag gcggtgatac ccgcaatatg
      601 cgccagttcg gcattgatgc ctggcagatg gaaggtgccg acaactatgg taacgtgcag
      661 tttacaggct attacacgcc ggtaattcag gcgcgccata cccgccaggg cgagttccag
      721 tatcctattt accgtatgcc gccaaaacgt ggtcgtctgc cgtctcgtgc ggagatctac
      781 gcgggggcat tgagtgataa atatattctc gcttacagta actccctgat ggataacttc
      841 attatggatg tgcagggtag tgggtatatc gactttggtg atggcagtcc gcttaacttt
      901 ttcagctatg cagggaaaaa cggtcatgcc tatcgcagca ttggtaaggt gctgatcgac
      961 cgtggcgaag tgaaaaaaga agatatgtcg atgcaggcga ttcgtcactg gggcgaaaca
     1021 cacagtgaag ccgaggttcg cgagctgctg gaacagaacc cgtctttcgt cttctttaaa
     1081 ccgcaatctt ttgcaccggt gaaaggggca agtgcggtgc cgctggttgg tcgcgcgtca
     1141 gttgcctctg atcgttccat tattccgcca ggtactacct tgctggcaga agtgccgttg
     1201 ctggataata acggcaaatt taatggtcag tacgaactgc gtctgatggt ggcgctggat
     1261 gtcggtggtg caatcaaagg ccaacacttc gatatctatc aagggatcgg gccggaagcc
     1321 ggacaccgcg caggttggta caaccactat ggacgtgtct gggtgctgaa aaccgccccg
     1381 ggcgcaggta acgtctttag cggctgatgt gttattctgt ataccgtttt tacggatacg
     1441 acagggtgag ggataacctc acccttttta attctgaggt gctatgtctg tggtaattag
     1501 tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca
     1561 actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc
     1621 ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg
     1681 cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc
     1741 ggaagtcatg gcggagcgta ttcgccagat taacccggag tgccgtgtaa cggtggtgga
     1801 tgatttcgtg acgccggata acgtagcgca gtatatgagc gtgggttatt cgtacgtgat
     1861 tgatgccatt gatagtgtac ggcccaaagc ggcgctgatt gcttattgtc ggcgcaataa
     1921 aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt
     1981 taccgatctg gcgaaaacga ttcaggatcc gctggcggcg aagttgcgcg agcgcctgaa
     2041 aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtcgatt gcgtgttttc
     2101 tactgaagcg ctggtgtacc cgcagtcaga cggtactgtg tgtgcgatga aagccacggc
     2161 agaagggccg aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc
     2221 cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcggcgcg
     2281 tcagggttaa acaatcaggt cggatgagac gcgaacgtct tatccgaccc ggttctatgc
     2341 ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcatttaacc cctggctgcg
     2401 tgaggcgcta agttgcgcac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc
     2461 ctgcaattcg gcggcggttt tcccctcaac ggcagtcaac agcaccgcca gcaggccgcg
     2521 cacaatgcgc ccttcgctat cgccaaagaa gtgcatttta ccgttttcag tcacggtata
     2581 tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc
     2641 tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg
     2701 cgacaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg
     2761 tccggcgaat tgcgggttcg tcattaatcc accaataatt ccagcgcgcg gtcaacggca
     2821 ttcaccagcg catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg
     2881 ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gttgcccggc ccgcagggca
     2941 ataccgtact ccgccagcag cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag
     3001 gccagcaggc tggaatcctg gcagcggaat gagcggaagc caggacgttt tgccagcgca
     3061 tcttccgcca gcgtcgccag gctgcgactc caactttctg cctggttgat atcgtaatct
     3121 gccagccatt ccagcgccgc gcttaatcct atgacaccag ccacatttgg cgttcctgct
     3181 tccagtttcc acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt
     3241 ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg
     3301 ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc gatatctagt
     3361 tgctgaacat ctgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc
     3421 ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc
     3481 gacatctgac ccaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg
     3541 acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg
     3601 gcgaccatca gccaggggac gaggttagcg tggtgttctg ccacgctgac aataatctca
     3661 tcgcccggtt gcagacgcgg acgcgcatag cattgtgcca ccatgttgat ggattcagtg
     3721 gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc
     3781 tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg
     3841 acgtttccgg cgctcaggct gtaaaactgt cgagtggctt caaccacggc ttcaggttta
     3901 agcgcggtcg cggcgctatc gagatagacg cccgcatcct gtagtgcggg aaactgggcg
     3961 cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga
     4021 cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg
     4081 cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc
     4141 aatactatta agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat
     4201 tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat
     4261 gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac
     4321 cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt
     4381 gaaagagatg gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca
     4441 attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa
     4501 aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca
     4561 tcacaaccgt aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg
     4621 ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat
     4681 ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa attaatttaa
     4741 catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt
     4801 gatgccgcag cacgccattt aagtttcact cgcgcagcag aagagctttt cgtgacccag
     4861 gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa actgttccgc
     4921 cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct cgatatcaaa
     4981 gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg
     5041 gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc
     5101 agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa
     5161 gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg
     5221 ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg
     5281 acaggcgaaa aacccttgaa gaccccggaa gatctggcta aacatacgtt attacatgat
     5341 gcgtcacgcc gtgactggca gacatatacc cgacagttgg ggttaaatca tatcaacgtt
     5401 cagcaagggc caatttttag tcatagcgcc atggtgctgc aagcggctat tcacgggcag
     5461 ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt
     5521 tgcccgttta atgatgttct ggtcagtaaa aacgcttttt atctggtttg tcatgacagc
     5581 caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct
     5641 gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc
     5701 agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct
     5761 tttggcgcgc atgtgttaag taaaaccatg ggggccgttg agatgggctg gatccagacc
     5821 ggcctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag
     5881 cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgttg
     5941 ttcagcggca gcctttattg cctggcgctg tcccatctgc gtttgtgggc atttgtcaca
     6001 ccggttggcg gcgtgagctt cctcgcgggc tgggcgttaa tgttagttgg tgctatccgt
     6061 ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga
     6121 aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc
     6181 ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga
     6241 taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt
     6301 gggggaactc ttgcagcatt tgccgccaga agatcgtatt acccccattg tcgacatgtt
     6361 gcagggcgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag
     6421 caaagagttg ctgaaattct gccgtaaatt caccgtgccg ctacgcgctg ccttgcgcga
     6481 tgccggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat
     6541 tgcaccaggc tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat
     6601 gggcattccg cgcctgaaat ttccggcaga ggcgccgagt cgttccacgc tcaaactgga
     6661 agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat
     6721 gtgggcggtg gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa
     6781 catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccgggca
     6841 ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acacgcagca atatctcctg
     6901 gatggtatgc gatatggttg aaaaaccggc taaagttgcg gcattgatgg cgcagtggct
     6961 ggttaatggc tggtgccgtg aaaccatttt caatctcaaa ctgccgatga aaaaacgcta
     7021 cgaagaagta tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa
     7081 tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg
     7141 ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg
     7201 tcgtcttcgg cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc
     7261 tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga
     7321 ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat
     7381 ttgtaatcag cgttgatcgc attggagtcc cacagtgcgc cgctgctatc gatcccttca
     7441 caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg
     7501 acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg
     7561 cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat gttgatgtca
     7621 ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct
     7681 tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc
     7741 agcctgatgt gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt
     7801 ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt
     7861 tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc
     7921 agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat
     7981 aaccgcccgc gtcatgcggg cgtaccgata tgagattacg gtgttacccc tttttttaaa
     8041 agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc
     8101 cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa
     8161 agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgctgcc
     8221 atcatcacca gcggcggggc ataactgtcc agctcaaata atgggataat cgcttggagc
     8281 aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga
     8341 aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat
     8401 agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc
     8461 aggttcagtt tgcgggtaga aataacggta ctgataatga atctgtgcac gggcttcttc
     8521 cgggctgtta aattccccta cgccatacca accgaacagc gcagctcctg cgacggtcgt
     8581 ttcggcatcg tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca
     8641 caatgtgttg cgacttcctc caccgactaa caataattca gaggctttaa agtgcccgat
     8701 tttttccagc atctgtagat tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg
     8761 atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga
     8821 caataaatca cactgcattt tcacaccatc cgcgccaggt gcgatcagac gagcttcttc
     8881 aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac
     8941 gccggatgcc agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca
     9001 ggtagaatcg gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggaccattaa
     9061 aatttcccat gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag
     9121 ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat
     9181 tgctgcggcg ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa
     9241 gagtcggcgt ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat
     9301 atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg
     9361 gtggttaatc agcgacgaaa taaagagcca ggcgtgcgcg cgttccagca gttgtggatg
     9421 attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga
     9481 aatagcctgc aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt
     9541 tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc
     9601 gccatccaca ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact
     9661 attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg
     9721 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
     9781 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
     9841 gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg cttatccggc
     9901 taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggagt
     9961 aatgtattaa cgcttgtaca acggaccgta gttctggcaa gcgcggtaat cctggccttc
    10021 aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt
    10081 gtgcatacat accgggatac gcagcatgga ggcgagagtg ataaagtctg cgccaacgtg
    10141 gccgatggtc agaaccccat ggttagcgcc ccagttcgcc attaccgagt acacatccgt
    10201 aaacggccct ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt
    10261 gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat
    10321 ttgcagtacc gatcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc
    10381 gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg
    10441 gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgtgg
    10501 cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga
    10561 acctgagttg atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt
    10621 tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt
    10681 gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc
    10741 cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc
    10801 ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat
    10861 ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg
    10921 catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg
    10981 ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc
    11041 agcccaggtc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg
    11101 acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt
    11161 gtgatcaaca atagaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt
    11221 acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac
    11281 atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc
    11341 cgggatgcct ttctggctgt gagctgccag agccgctgcc aggtaaacag cgccgggacg
    11401 ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc
    11461 actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct agctgctgaa
    11521 tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca
    11581 ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt
    11641 cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat
    11701 aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta
    11761 ttcggaaaat atgttcagtt agttgccgtt tgagagcgga aacgggcaaa gataaagatg
    11821 accgcgaagc agagtgtggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg
    11881 acaaaaccca tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac
    11941 gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac
    12001 tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg
    12061 aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt
    12121 ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg
    12181 gttccggtta aatagttagc ggcaaagcct gcagtcatac ctggaatttc ttctacagcg
    12241 tagcgaatca aatagctcca gcaggccgtt tgtgcgccaa cgtagcagaa ttgcgctaat
    12301 actgcccagc gccagtggcg aatacgcgct aggcgagaaa gcgatgcgga gaacgatcct
    12361 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    12421 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
    12481 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    12541 tgatgtggca cgttagacaa aataagtctt tgcccaaaga caaccgcgat aattgcgcca
    12601 aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactgctttc cggccctaat
    12661 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    12721 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    12781 cccagggcat ataaaaataa cccagtaata attcctgctt tataactgag ttttttcatc
    12841 aatatcccag caggaattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    12901 ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
    12961 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    13021 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    13081 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    13141 aaaagtgcaa aaccagcgag ttcgggcttc atatcagtga atatttgcct gtctgaaatg
    13201 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    13261 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    13321 ttcagaaaat aaatcataaa aaacacaaac cagatgactg tattaattgt ttatgccgaa
    13381 aacgggcatt cctggcttgg gtgaatagaa aaatgaccgt tttgataaat atttttaaga
    13441 gcggtcattt ttaatggggt tataaaaaga gaacttgcag ttatttactg tgattagttg
    13501 agctaggtca caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt
    13561 gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa
    13621 tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc
    13681 tggaaatgac ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc
    13741 aggatgggat gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata
    13801 ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc
    13861 gtttccatat ggcggcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg
    13921 ccgttcattg cacagcagtt tccattctta accgaccgat ccccgctatt cactacatga
    13981 ttgcggcggc aggtggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg
    14041 aactttctga acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc
    14101 atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg
    14161 aagtgctggc gcaactttac gtggcgaccc tggcgattac ggacccggtg ccagtgctga
    14221 gcgatgaaga gattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag
    14281 agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa
    14341 cggcatggtt tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt
    14401 atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag
    14461 tgaccgataa gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca
    14521 acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt
    14581 acctgatcgc tattggtggt ggttctccac aggatacttg taaagcgatt ggcattatca
    14641 gcaacaatcc ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac
    14701 ccagtgtacc gattctggca attcctacca cagcaggtac tgcggcagaa gtgaccatta
    14761 actacgtgat cactgacgaa gagaaacggc gcaagtttgt ttgcgttgat ccgcatgata
    14821 tcccgcaggt ggcgtttatt gacgctgaca tgatggatgg tatgcctcca gcgctgaaag
    14881 ctgcgacggg tgtcgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt
    14941 gggcgctaac cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag
    15001 gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg
    15061 gtatgggctt ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg
    15121 cgttttacaa cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcact
    15181 ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag
    15241 tggaaggtat gagcctggaa gaggcgcgta atgccgctgt tgaagcggtg tttgctctca
    15301 accgtgatgt cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattc
    15361 cggcactggc gcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa
    15421 cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg
    15481 ccggatacga cgctagccgc gtcttatccg gtatataagt gcattaaatt caatgtgttg
    15541 ataatcgttt gcatccggct cgccgttacc gtaccaaccg caattgctgt aaattgccgt
    15601 cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc atctctttat
    15661 gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca tatatccctt
    15721 ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt cccgcaacac
    15781 ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac tgctgcggtt
    15841 gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg aagtaatcac
    15901 gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa acaatcgttg
    15961 cctgatgccc ggcctgtgtc actttgaccg ccagcgtggc ggctaagtca tcggcttcgt
    16021 tgccgctggt tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt aatgcaggca
    16081 tctcgtcgtg caactcttcc ggcattggcg gtcgacccgc tttgtaatct ggtaaacgct
    16141 gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg tgggttggct
    16201 ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag ggcgatccct
    16261 gaacggcatg aatgcgacga ataagattca gtgcatcgac aataagcaaa tgaacagcca
    16321 cgataacccc cttaaagatc aggcgataag ggtaacattc gatgccggaa caggctacgg
    16381 ctggatgaga aatcgggaag aggcctcgca aaacgaggcc tctggagagc gattaatcgc
    16441 aggcaacgat cttcattgcc aggccgccgc gagaggtttc gcggtacttg gcgttcatat
    16501 ctttacctgt ttcgtacatg gtttcgataa ctttatcgag gcagacgcgc ggctcgctgg
    16561 tacggcgtag cgccatacgt gcggcgttca ccgcttttac tgccgcaatg gcgttacgct
    16621 cgatgcatgg cacctggacc tgtccggcga ccgggtcaca cgtcagaccg aggttgtgct
    16681 ccatggcgat ttccgccgcg atgcacacct gcgccgggct tgcacctaac agttctgcca
    16741 gaccagccgc cgccattgag cacgccacac caacttcacc ctggcagccg acttcagcac
    16801 cagaaattga cgcgttcatc ttataaagag aaccaatggc actggctacc agcaggtaac
    16861 gagccagtga gttagcgttc acttcgcgga tgaacttgtc gtagtacgcc agcactgccg
    16921 ggataatccc gcacgcaccg ttagtcggcg cagtcaccac gcgtccgcca gcagcgttct
    16981 cttcgttcac tgccagtgca aacatgttga tccagtcaac aaccgccatc gggtcagtgg
    17041 tggttttatc ctggctgacc agcatccggc gtagtgccgc agcacggcgt ggaacgcgca
    17101 gtttgccagg caacacgcct tcggtggaaa taccgcgctc aataccgcca cgcatgactt
    17161 cccatacgtt cgccaggtgc tgttccagct cttctttgct gtgcagcgcc agctcatttt
    17221 tcatcatcag gccagagagt gacagcccgg tttcctgaca atgtttttgc agatcggctg
    17281 ctgaactgta cggataagga acttcaaccg gtgcgctatt ctgctggcca aaatgctctt
    17341 catcaacgat aaagccaccg ccaatagagt agtaagtctg gctgtaaacg actttatcgc
    17401 ccgccagcgc ggtaatgcgc ataccgtttt catgcagaga aaggttgtct gcatggaagt
    17461 tcatgcactg atcaaccggg aactccactt catgctgacc gtttgccagc atcaggcgac
    17521 catgagtatt cacatcctga ataaaaccag ggatggaatc gatatccacg gtatccggca
    17581 ggttacccgc caggcccata ataatggcga tatcagtgtg gtggccttta ccggtcagag
    17641 agagcgagcc gtacacgtca accaccacgc gggtcacgtc tttaagcaga ttacgggcaa
    17701 tcagatcgtc ggtaaattgt ttacccgctt tcattggtcc aacggtatga gaactggaag
    17761 ggccaatgcc gattttgaaa atatcgaata cgctaatcat aggaaataca tcgtgttaaa
    17821 acggaggaag cgccgcccga aagcggcgcg aaaggactta gctgaacaga gagtagaaga
    17881 ttgcggagat tgcaatcaga cccatcacga caacgaatac gttgctgatg tgaccgctgt
    17941 acttacgcat tgccggtact ttctgaattg cgtacatcgg catcaggaac aggatcatcg
    18001 cgatgattgg gccgcccagg gtttcaatca tacccaggat gctcgggttc agggtggcaa
    18061 caatccaggt cgttaccagc atgaacagcg cagtgatacg gttcagcttg ttgatttcga
    18121 tagacttacc tttaccacgc agagatttaa tcaccatacc gttgaagcct tcacgtgcgc
    18181 ccaggtagtg gccgaggaag gatttggtga tagcgataat cgcgataatc ggagccatcc
    18241 acgcgataac cggtgcgtta aagtggttag ccaggtaaga cagaatcgag atgttctgct
    18301 ctttagccgc agccaggtct gccggagtca ggctcagtac acagctgaag acgaagaaca
    18361 ttacggtcag caccatcatg atgtgtgcga atgccaggat cttcgagcat ttctgttctg
    18421 ccatatcgcc gtactcttca cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt
    18481 tgaaagagaa caccataacc ggaattgcca gccacagggt catccacaga ccgtttccgg
    18541 ttgcagatgc agtgtccaga gacagcgttt ccagtgctgc gccgttccac tgcgggatca
    18601 ggtacagagc cagcagcatc agtacgccaa caaacgggaa taccagaata ctcattgctt
    18661 taacgatcat ctgctcaccg aagcgaacga tggtcatcat acccacgatc aggatcagcg
    18721 acagaatcgc acgcggcggt ggtgtcatac ccagctggtg agacatgaag ctttcaactg
    18781 tattggtgat tgccacgcta taaaccagca ggatcgggta gatagcgaag aagtagagca
    18841 gggtaatcag tttacctgcg ccaataccaa agtgttcttc tacaacctcg gtgatgtctt
    18901 cgcctgggtt tttaccagac agtacgaagc gagtcaggcc gcggtgagca aaaaatgtca
    18961 tcgggaacgc aaggatagcc atgatgatca gcgggatcat accgccaaca ccggcgttga
    19021 ttggcaggaa cagcacgccc gcgccgattg ccgtgccgta aaggcccagc atccacatgg
    19081 tgtctgtctt gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg
    19141 tttccatcta tttctcctgg aggaatgcaa ataattcagc ttttagccag ttcagctcgg
    19201 gaaggtgcct gaccgtaaaa atgaaatctg tctatcggcg atggcttaat catttaccgc
    19261 cgtaataatt tgagggcgga aagatacaca caagttatgt atctatcagt gatcttgatc
    19321 ccaaatacaa taatctactt ggaagataga ttatcccaac gctttactct gtaaatcaaa
    19381 agtaaaagag aatttaaggg caatatttgc ccattaaacg acatctgact tcttccgggg
    19441 cgcgcaatat atgtaggttg aactgaaaaa atcaatactt ttagttaatc atggcgataa
    19501 tttctttttc atagcgataa aatgtggttt atgaaaatag ggaaacgttt gcgttacaac
    19561 ataaattgta tgacatatta actgcaatga tatttatcgc gtgagggtgg taagaaacat
    19621 tcatataaca aatgaaataa attgatgggt atgtgaccaa aaacgagaaa acccggcaac
    19681 aaacgtcacc gggggaggga gattacgtgc agatttcgta gcaagggatg taggctgagc
    19741 ctggcaactt catacgatgc tgggcaacaa aaccctgtag caggtcgtcc atacgacgca
    19801 taatctcttt atcgccgttg attttgtaag gaccaaactc ttcaatggcg cgaataccga
    19861 cttcttttac gttacccgcc acaataccgg agaacgcacg gcgcagatcg gcagccagca
    19921 cttcaacagg ttgatccggg taaagcttca gattagccat attctcgtga gacggctcaa
    19981 acggcatttg caaatctggc gcaatgcgca ttgaccagtt aaagctgtag gcatcgcctg
    20041 tgtcgcggcg attttctttc accagcggca tcgatttttt catctgacga gcgacttcag
    20101 cggcgtcatc aatgatgatg cggtaatggc ggcgcgcgtt ttcacccaac gtatgcacga
    20161 caaactcgtc cagtacgcgg aagtagtcgg cgctctcttt cgggccggtg aggaacaatg
    20221 gtaaaacctg atctttgttg gccgggttca ttaaaattcc cagcaaatac agcaactctt
    20281 ctgccgtacc cacaccgcca gggaagataa tgatgccgtg agcgatacgg acaaacgctt
    20341 ccagacgttt ttcgatatct ggcatgatga tcaattcgtt gaccagcggg ttaggcggtt
    20401 cagcggcgat aatcgacggc tctgtcatac caataaaacg actgtctttg taacgctgct
    20461 gtgcgtgtcc gaccgcagca cctttcatcg gcgcttccat cgctcccgga ccacagccgg
    20521 tgcagatatt cagctcacgc aggcccagct gattcccgac gcgacgggca tacaaatact
    20581 cgttttcgtt aattgagtgt ccgccccagc agaccaccat atttggcgct tcaccgacat
    20641 gcagtgcgcg agcgttacgc aagatggaaa agaccaggtt agtgatgtgg acggagttgt
    20701 cgagattcag gttgggaaaa cgaacggtgt tatggatttg cccgtaaacg aagagaatgt
    20761 cacgcagaac cgcgaacaag ttggcctgca acgcgcgaat aattcgccca tcgacaaaag
    20821 cctcttccgg gggattaatc agttccagct ttacgccgcg ttcacggcgc aggacgttaa
    20881 tatcgaaatt ttcaaaacga gacagcaatt ctttgctgtt atcggtcaaa ctaccggagt
    20941 tcagtacggc aagtgaacag ttgcgaaaca gttgatagag gtcgctgctg gcggtgcgtt
    21001 taagcatatc cacttccagc tgcgacaaca tatccatgga gccaagcggg ctaatatgtg
    21061 taatcaagaa aaactcctta tgggacgaat atcgtcattc cctttgatta caatagccct
    21121 gcctgatgtc ctttcacaac cttctgcgtg aatccagcac gcaaaattga gaaaaaattt
    21181 attgccgaac cagtcttgtg gtcgatggga caaaatcgct attactgcgc cacgggttaa
    21241 tgtccagacc gccacgacgg gtataacgtg cgtaaacgct caatttttct ggctggcaga
    21301 agcgtaacag gtcattaaag atgcgttcca cgcactgttc gtggaactcg ttgtgatgac
    21361 ggaatgagac caggtaacgc agcagttttt ctctgtcaat ttggcgtcca cgatactgaa
    21421 tttggatcga accccaatct ggttgatggg tgatcaggca gtttgatttc agcaggtggc
    21481 tgacaagcgt ctcttccact actttttcac cacaggtggc attctccaga tagtcagtag
    21541 tgaattcata gttatcgata gtgatatcct ggtcatcaat gcaagtgcca ttaaaatgac
    21601 ctatcggctg gccttccagt tcatcaagac gatataacgc cacgctaatc ttaccctgag
    21661 cgcaagtgct taagtcgcgc tccagcgtct ggcgcacctc atcccagtta ttaaaacgcg
    21721 tctggttaaa actgttgaga tagagcttaa aactcttcga ctcaatcaga tttacgctgg
    21781 tgtaatcaag ttcaacatga ccgacagcga cctgcggcaa acctttcgca ttcagccagg
    21841 aaagttcata cagcgtccag atatccgtac cgtgaaaagg caggttatcc gctttcagac
    21901 ccagcgggtc gcgattcagg ctgcgtggaa cgccttgcag taggctggcg tcataggtat
    21961 cccggtaatc ggttgatttt ccaagagtca ggcccgcaag tgcctgatgg tttgcataag
    22021 aagacatgtt tcaccgtcat aaaccagata aactaaacga caagtgtatc tggtttaagt
    22081 gaagagagaa attagtggac gatttgaccg cacaagccct gaaagatttt actgcacgct
    22141 actgcgatgc gtggcatgaa gagcataaaa gctggccgtt aagtgaggaa ctgtacggtg
    22201 ttccttcacc gtgcattatt tcaactaccg aagatgccgt atactggcaa ccccagccgt
    22261 ttaccgggga acaaaatgta aacgcggttg aacgcgcttt tgatattgtg atacaaccca
    22321 caattcatac cttttatacg actcagtttg ccggggatat gcacgcgcag tttggcgata
    22381 tcaaactgac attgctgcaa acctggagtg aagacgactt ccgtcgggtg caggaaaatc
    22441 tgattggtca tctggtgact cagaaaaggc taaaactgcc gcccacgcta tttatcgcga
    22501 cgctggaaga agagttagag gtcatctcgg tgtgtaatct ctccggtgaa gtgtgcaaag
    22561 agacgctggg aacacgtaaa cggacgcatt tagcctcaaa tcttgcggaa ttccttaatc
    22621 aacttaagcc tcttctgtaa tccaaatacc ccatctggtt gtgagagatc tcttacagac
    22681 cctgtaggag atcgccagga aataagcgaa tacttaaaaa gataagaatc gttattttca
    22741 tttaaaatca aaatgttgat tgttaattct taactttcat atgaaatttt ccttaaggca
    22801 ttgtctgtaa gcgccttgta agacttcgcg aaaaagacga ttctatcttc gtcgacaggg
    22861 agtcagacaa cgaagtgaac atcaggatga tgacacttct gcaggacaca ccaggatggt
    22921 gtttcaggga aaggcttctg gatgaagcga agaggatgac gcaggacgcg ttaaaggaca
    22981 cctccaggat ggagaatgag aaccggtcag gatgattcgg tgggtcagga aggccaggga
    23041 cacttcagga tgaagtatca catcggggtg gtgtgagcag gaagcaatag ttcaggatga
    23101 acgattggcc gcaaggccag aggaaaagtt gtcaaggatg agcagggagc aacaaaagta
    23161 gctggaatgc tgcgaaacga accgggagca ctgtgaatac agtgctccct ttttttattc
    23221 ctgctatcct tcgcggcagt ttttcttatt gaagttgctt tatgaccact catgaccgcg
    23281 ttcgtctcca gttgcagacg cttgaagcgt tactgcgtga acatcagcac tggcgaaacg
    23341 atgaacccca gccgcatcaa tttaatagta cccaaccgtt ctttatggac accatggaac
    23401 cactggagtg gttgcagtgg gtgctgatcc cgcgaatgca cgatttgtta aataacaacc
    23461 agccgttacc gggcgctttt gctgttgcac cctattatga aatggcgctg gcgacggacc
    23521 atccgcaacg cgcgcttatt ctggctgagt tggaaaaact ggatgctctt tttgcggatg
    23581 atgcgagcta atgctggaaa tactctatca ggatgaatgg ctggttgcgg taaataaacc
    23641 ctccggctgg ctggttcacc gtagctggct ggatcgcgac gagaaagtag tggtcatgca
    23701 aaccgtgcgt gaccagatag gacagcatgt ttttactgct catcgtctgg accgacccac
    23761 ttctggtgtg ttgttgatgg gattatccag cgaggccgga cggctgctgg cacaacagtt
    23821 tgaacagcac caaatccaga aacgttacca tgcgattgtg cgcggctggt tgatggaaga
    23881 agcggtgctg gattatccac tggtggaaga actggacaaa atcgctgata aatttgcccg
    23941 cgaagataaa ggcccgcagc cagcagtgac gcattatcgc ggtctggcga ccgtagaaat
    24001 gccggtagcg accggacgtt acccgaccac gcgctacggc ctggtggaac tggagccgaa
    24061 aaccggacgc aaacatcagc ttcgccgaca tcttgcccat ttgcgtcatc cgattattgg
    24121 cgatagcaaa catggcgatt tacgccagaa tcgcagcggt gctgagcatt ttggcctcca
    24181 gcggttaatg ctacatgcca gtcagttgtc attgacgcat ccttttactg gcgagccgct
    24241 gactattcac gcgggcctgg acgacacctg gatgcaggcg ttatcacaat ttggctggcg
    24301 cggtctgctt cctgaaaatg aaagggttga gttcagtgcg ccatcgggtc aggatggaga
    24361 gataagttcc taatccaggg agaaaacggt atggcggaaa ttggtatttt tgtcggcacc
    24421 atgtacggaa attcgctgtt agtggcggaa gaagcagaag caattctgac cgcacagggc
    24481 cacaaagcaa cggtatttga agatcctgaa ttaagcgact ggctgcctta tcaggataag
    24541 tatgttctgg tggttacgtc cacgaccggg cagggcgacc ttcctgatag cattgtgccg
    24601 ctctttcagg gaatcaaaga tagtctgggc ttccagccga atctgcgtta tggcgtgatt
    24661 gcgctcggcg acagcagtta tgtaaatttc tgcaatggcg gcaaacagtt cgatgccttg
    24721 ttgcaggaac agagcgctca gcgggttggc gaaatgctgt tgattgacgc cagtgagaac
    24781 ccggaaccgg aaacggaatc aaatccgtgg gtcgaacagt ggggcacgct gttgtcctga
    24841 tatgttcagc gagcggtaaa tgtcgtttta gcggtgctga atcgaatctt ttcaggcaaa
    24901 tgccagtaaa aactgcttca tagcgcggat ttttactggc gtttgcctgg agtcaagcga
    24961 tccatttcat actcttcttt atttcttcgt tttaaccctt cctttcttgt tcttgttttc
    25021 atttccgtga agtggattcc accgtccagg gctaatgcca aaatcgggcc tcattgaacg
    25081 cattaatgtt gtgttgttgc acggtgagcc gctatggcgc gctttttata ctgctattgc
    25141 cagatataaa cacgcgccgt attcggcgaa cgacctataa aaacggcaaa aaacacccta
    25201 cgtcacctct gatttcctgg ctatgtcgca gtccagagtg agcgtggcta acgcgaattt
    25261 tcaggagtgc aacaatgagt tctttaagtc aggctgcgag cagtgtggaa aaacgcacga
    25321 atgctcgtta ctggatagtg gtgatgttgt ttatcgtcac atccttcaac tacggcgacc
    25381 gcgctacgct ttctattgcc ggttcggaaa tggccaaaga tatcggcctt gatcccgtgg
    25441 gaatgggcta tgtgttctct gccttctcat gggcttatgt tatcgggcag atccctggtg
    25501 gttggttgct ggatcgtttt ggttcaaaac gcgtctactt ctggtcgatc tttatctggt
    25561 cgatgtttac cttgctgcaa ggcttcgtcg atatctttag tggattcggc attatcgttg
    25621 ccctgtttac tctgcgcttc ctggtcgggc ttgctgaagc gccatctttc cccggcaaca
    25681 gtcacattgt tgcggcctgg tttccggcgc aggaaagggg aacggcggtg tcgattttta
    25741 actccgctca atacttcgca acggtgatct tcgcgccgat tatgggctgg ctgacgcatg
    25801 aagtgggctg gtcacacgtc ttcttcttta tgggcggtct ggggattgtc atcagcttta
    25861 tctggttgaa agtcatccac gagccaaatc aacatccggg ggtaaatcag aaagagctgg
    25921 agtacatcgc cgcgggtggc gcgctgatca atatggatca gcaaaacacc aaagttaaag
    25981 tgccgttcag cgtgaagtgg gggcagatca aacaactgct cgggtcacgg atgatgatcg
    26041 gcgtttatat cggtcagtac tgtatcaacg ccctgactta ctttttcatt acctggtttc
    26101 cggtttatct ggtgcaggcg cgtgggatgt cgattctgaa agcgggcttt gtggcttccg
    26161 ttccggcggt ttgcggtttt atcggcggtg tgctgggtgg gattatttcc gactggctga
    26221 tgcgtcgcac gggatcgctg aacattgcgc gtaaaacacc gatcgtaatg ggcatgttgc
    26281 tgtcgatggt gatggtgttc tgcaactacg tcaacgttga gtggatgatc atcggcttta
    26341 tggcgctggc cttcttcggt aagggcatcg gggcgctggg ttgggcagta atggcagata
    26401 ccgcgccaaa agagatcagc ggtctttccg gtggcctgtt caacatgttc ggtaacattt
    26461 ctggcatcgt cacgccaatc gcaattggtt atatcgttgg cacgactggc tcctttaatg
    26521 gggcgctgat ttatgttggt gttcatgcct taatcgcggt actgagctac ctggtgctgg
    26581 tgggcgatat caagcgtatc gagctgaaac ctgtcgcggg gcaataagat gacgacacaa
    26641 tccagtcctg ttattactga tatgaaagtc attccggtcg ccgggcacga tagcatgttg
    26701 cttaatattg gtggggcaca taacgcatat ttcacccgca atattgtggt actcaccgat
    26761 aacgccgggc ataccggcat tggtgaagcg ccgggcggag aggtgattta tcaaacactg
    26821 gtcgatgcta ttccgatggt tctgggccag gaagttgcgc gcctgaataa agtggttcag
    26881 caggtgcata aaggtaatca ggcagccgat tttgatacct tcggcaaagg tgcctggact
    26941 tttgaattgc gcgttaatgc cgtggcggcg ctggaagccg ccttgcttga cctgctaggt
    27001 aaggcgctga atgttccggt ctgcgaactg ttggggccag gcaagcaacg cgagactatt
    27061 accgtcctcg gttatctgtt ttatatcggt gatcggacca aaaccgatct tccttatctg
    27121 gaaaatacgc cgggcaacca tgagtggtat cagttgcgcc atcagaaagc gatgaacagc
    27181 gaagccgttg tgcgtctggc ggaagcctca caggatcgct atggctttaa agatttcaaa
    27241 cttaagggcg gggtgttacc tggcgagcaa gaaatcgaca ctgttcgtgc attgaagaaa
    27301 cgcttccctg atgcgcggat taccgttgat cccaacggtg cctggctact tgatgaagcc
    27361 atttctctat gcaaagggct gaatgatgtt cttacctatg ccgaagatcc atgcggcgca
    27421 gaacagggtt tctccggtcg tgaagttatg gcggagtttc ggcgggcgac cggcttgccc
    27481 gtcgcgacta acatgatcgc caccaactgg cgcgaaatgg gtcatgcggt gatgctcaat
    27541 gcggtagata ttccacttgc cgatccgcac ttctggacgc tttccggtgc agtccgtgtg
    27601 gcgcagcttt gcgacgactg ggggctgacc tggggctgcc attctaataa ccatttcgat
    27661 atctctctgg cgatgtttac ccatgtgggc gcggcggcac cgggtaatcc taccgctatc
    27721 gatacccact ggatttggca ggagggcgat tgtcgcctga ccaaaaatcc gctggagatt
    27781 aaaaacggaa aaattgccgt tcctgatgcg cccggtctgg gcgtggaact ggactgggaa
    27841 caggtacaaa aggcacatga ggcctataaa cgtctgcctg gcggtgcgcg taacgacgca
    27901 ggtccgatgc agtacctgat ccccggctgg acctttgacc gtaaacgtcc cgttttcggc
    27961 cgtcattgat tctgaaaaag gacataaata tgagttctca atttacgacg tctgttgtta
    28021 ctgaaatgca ggtcatcccg gtggcgggtc atgacagtat gctgatgaat ctgagtgggg
    28081 cacacgcacc gttctttacg cgtaatattg tgattatcaa agataattcc ggtcacactg
    28141 gcgtagggga aattcccggc ggcgagaaaa tccgtaaaac gctggaagat gcgattccgc
    28201 tggtggtagg taaaacgctg ggtgaataca aaaacgttct gacgctggtg cgtaatactt
    28261 ttgccgatcg tgatgctggt gggcgcggtt tgcagacatt tgatttgcgt accactattc
    28321 atgtagttac cgggatagaa gcggcaatgc tggatctgct ggggcagcat ctgggggtaa
    28381 acgtggcatc gctgctgggc gatggtcaac agcgtagcga agtcgaaatg ctcggttatc
    28441 tgttcttcgt cggtaatcgc aaagccacac ctctgccgta tcaaagccag ccggatgact
    28501 catgcgactg gtatcgcctg cgtcatgaag aagcgatgac gccggatgcg gtggtgcgcc
    28561 tagcggaagc ggcgtatgaa aaatatggct tcaacgattt caaactgaaa ggcggtgtac
    28621 tggccgggga agaagaagcc gagtctattg tggcactggc gcaacgcttc ccgcaggcgc
    28681 gtattacgct cgatcctaac ggtgcctggt cgctgaacga agcaattaaa attggtaaat
    28741 acctgaaagg ttcgctggct tatgcagaag atccgtatgg tgcggagcaa ggtttctccg
    28801 ggcgtgaagt gatggcagag ttccgtcgcg cgactggcct gccgactgca accaatatga
    28861 tcgccaccga ctggcggcag atgggccata cgctctcgtt acaatccgtt gatatcccgc
    28921 tggcggatcc gcatttctgg actatgcaag gttcggtacg tgtggcgcaa atgtgccatg
    28981 aatttggcct gacctggggt tcacactcaa acaaccactt cgatatttcc ctggcgatgt
    29041 ttacccatgt tgccgccgct gcaccgggta aaatcaccgc gattgatacg cactggattt
    29101 ggcaggaagg caatcagcgc ttgaccaaag aaccgtttga gatcaaaggc ggtctggttc
    29161 aggtgccgga aaaaccgggg ctgggtgtag aaatcgatat ggatcaagtg atgaaagccc
    29221 atgagctgta tcagaaacac gggcttggcg cgcgtgacga tgcgatggga atgcagtatc
    29281 tgattcctgg ctggacgttc gataacaagc gcccgtgcat ggtgcgttaa gacagaaaac
    29341 caaaaccggc tctatctgga gccggcttga gactgatgct aaacgcaaaa tagcctgttg
    29401 tgctatgtat caggtctaca tcatttttta attcattgaa tttaattggt tttgtaggcc
    29461 ggataaggcg ttaacgccgc atccggcata aacacaggca ctttgtcacc aatctgaaac
    29521 cagcgtcata aaaagccgat tgctactcga caagacgtcc gttacccgag aattttgctg
    29581 gcttcgcgcg cgacattatc catctcgtcc aacagttcca gcagctccgg ctccaaatct
    29641 tcttctttag taccgctacg tagctgttgt tcgatcagtt ggcagagatt cttcatacgc
    29701 ggcacaccgc tatagccgca actgccatgc agtttatgaa tcaaatcaac caggccttcc
    29761 gggttttctc caaccagctg ctcctcaact ttgttgcgaa cttcaggcag gaaatcgagt
    29821 aacatttgca gcatatcgcg cgctagatcg gtttttcctg ctgcctggcg tagtgccagt
    29881 tgccagtcga gggtcgcatt cgggttcacc acaatttcgt tgacttcggg cgtcacgacg
    29941 cgagaggaaa taccgctgcc aggcttgtag cgcaacaaca aattatgcaa tcgctcttct
    30001 tcaatcggtt tcgccagata atcgctcatc cctgcgccaa gcagcttctc tttttgcccg
    30061 gccattgcat gcgccgttac cgcgataacc ggcgtttgtt gctgatgggg gagctggtgg
    30121 atgagctcgc aggcccgaat gccatccatg tcaggcattt gaatatccat taagatcaaa
    30181 tcgaacggca tctgtttcgc ccgttcaacc gcctgatgcc cgctatcgca aagttccaca
    30241 tgttgcacca tatcttccag caatgcgccg ataagtttca ggttagcggg gttgtcatca
    30301 actgccatga ctgtcattgc cagcttgctt tcatcggtta caggcaaaag cgtgttttgt
    30361 ttgtgatgac aaaattccgt cagggcaggc aacaggcgcg taggtgttaa tggtttcagc
    30421 agacacgcgc cgataccatc ttgcttgagt ttttctgcat tgacttgtgc atggcaagga
    30481 agtgccagca tcaggaaatc ggtcatcgat accgctttcg ctaatcgctc atgttgcatt
    30541 gttagcggct cgcggaaggt caccgcgatg cctaacaaca tcatgtcgta atgcgcggga
    30601 ggcagcgcgg agaacgttgg gctataaacc acttccagcg gcgtttcact taaaatatcc
    30661 agcgtgcatt gcgctgctgc ggagtttggt tcgacatagg ccaggcgctt accggcgagg
    30721 cactgggtgg atggcccttc gataataatg ttcgggttca gatcgagatt aatgtggaac
    30781 cagaaagttg aaccgcgatt cggctggcta tggaacgaaa tatcgccgcc catttcatta
    30841 accagttttt gtgtaatcac cagccccaga ccggtgccac catgacggcg ggaaatactg
    30901 gcatcagcct gtcggaaggc ctggaataag cgcgattgat cgcgttcagg aataccaatg
    30961 ccggtatccc gaatctgcac ttcaatctgc actttggtat tactcagcgc acgtttttct
    31021 accagaatat caatgttgcc attctcggtg aatttaattg cattccccac caggttagtg
    31081 atgatttgct gtaatcgcag tgggtcgccg atcacgttat caggcacgtc gcttttaata
    31141 ttgagcgtca gttccaaccc tttatcgtga gaagaatgtg ccagcagagt aacgacttca
    31201 tccagcgtgc tgcgtagtgg gaatggaata ctttccagaa tcagcttacc tgcttccagt
    31261 ttcgagaagt cgagaacatc attaataatt gccagcaaat tatttgccga acgttcaatc
    31321 gtattcaggt gatcgcgctg cgttggtgtt aattctgttt tcagcgtcag gcgggtaaag
    31381 ccaataacac cattcagtgg tgtacgcagc tcgtgtgaca tatttgccag aaactcggat
    31441 ttaatacgcg ccgcttcctg ggcgcgcttt ttcgccagat ctaactcaac gttctgaatt
    31501 tccatctgct ccagcgtttc acgcagatcg gacgtcgcct ggtcgatatt gtgctgcatc
    31561 tcttcgtgat aagcagccag cgacattgcc atcgagttga taccgttttt cagcatatcc
    31621 agctcgccga gcataaatcc ttccacccgg ctgtcgagtt gcccgcgacg gatgcggtcg
    31681 acggtattca ccatgttgcg aatcggaccg gttacatcgc gcattaagcg ccagccaaaa
    31741 ataagcgcaa taccgataca aaacagcatc atcacgctgg aaataaagat ctctttatat
    31801 tgctgcaagc gaaccgattt aagatccagc tccagcgcaa tatatcccaa catattttga
    31861 ctatttttgg catcgctact gggcgattcg tcgggggagt aactctcaga aataatcggc
    31921 gtgcggagga tcataatatc gccgtcacga gtgacagtga gctggcgagg aaacggcacg
    31981 ttgctgccga gctgcattga tgagggatca agatgaaaat tggaggtgac aaagagtcgg
    32041 ttattttcat cataaaccga aatcgcgcga acaatatcgg aatggcgacg atgcagtacg
    32101 cttattaact gaccgataga ttcgcgattt tgcaggctca tgccatattc agtagaaact
    32161 gcaagcggct caataatgct ggctccggca tcttccagtt gacgctgcaa gtcgttatag
    32221 cgatgcacaa cgaaaaagat actcagcaat aaaccaataa ggacggtcgg tgccaggatc
    32281 agaatcatca tgcgtgcgcg caggctgtag ttggtcatgg agttccgtta tgggacaatt
    32341 aaggtcacac tgttaaattg agaaagtctc gccaatggcg caattctact ctgcaaaacg
    32401 acgcacgacg acgcgtcaga tcataaccgt ttcagtcaac gacctcgact cttttggtca
    32461 gggcgtggcg cgacataacg gcaaaacgct atttatcccc ggattattgc cgcaggaaaa
    32521 cgcggaagtt actgttactg aagataaaaa acagtatgcc cgcgctaaag tcgtacgccg
    32581 gttaagcgat agcccgggac gcgaaacgcc acgctgtcct cattttggcg tatgcggcgg
    32641 ctgtcagcaa caacacgcca gcgtggattt acagcagcga agcaaaagtg cggcactcgc
    32701 ccgattaatg aaacacgaag tctctgaagt gatcgccgat gttccctggg gctatcgccg
    32761 tcgcgcgcgt ttaagtctga actacttacc gaaaacacag caacttcaga tggggtttcg
    32821 caaagcgggc tccagtgaca ttgtcgacgt taaacaatgc cccattttag tgccccaact
    32881 tgaagcattg ctgcccaaag tcatggcatg cttgggcagc ttacaagtta tgcgccatct
    32941 tggtcatgtt gaactggtac aggcaaccag cggcacgctg atgattttgc gccataccgc
    33001 accgctaagt tcggcagatc gcgaaaaact ggaacgcttt tcgcattctg aaggcctgga
    33061 tctgtatctc gcccccgata gtgagatact cgaaaccgtc tctggtgaga tgccctggta
    33121 tgactcaaat gggttgcgct taacttttag cccgcgcgat tttattcagg tcaatgcggg
    33181 tgtgaaccaa aaaatggtag cgcgtgcgtt ggaatggctg gatgtgcaac ctgaagatcg
    33241 cgtactggat ttgttctgcg gtatgggcaa ctttacactg ccattggcga cacaagctgc
    33301 cagtgtggtg ggtgtagaag gcgttccggc gctggtggaa aaaggccagc agaatgcgcg
    33361 tcttaatggc ttacagaatg tgacgtttta tcacgaaaat cttgaagaag atgtcacaaa
    33421 gcagccgtgg gcgaaaaacg gcttcgataa agtgttgctg gacccggcgc gagcaggtgc
    33481 cgcaggtgtt atgcagcaaa ttataaaact ggaacctatt cgtatagttt atgtatcctg
    33541 taaccctgca acgctggctc gggatagcga agcgttatta aaagcaggat ataccattgc
    33601 gcgactggcg atgctggata tgttcccaca cacgggacat ctggaatcga tggtactttt
    33661 ctcgcgcgtt aaatagttgc gatttgccga tttcggcagg tctggtccct aaaggagagg
    33721 acgatggttg cggtaagaag tgcacatatc aataaggctg gtgaatttga tccggaaaaa
    33781 tggatcgcaa gtctgggtat taccagccag aagtcgtgtg agtgcttagc cgaaacttgg
    33841 gcgtattgtc tgcaacagac gcaggggcat ccggatgcca gtctgttatt gtggcgtggt
    33901 gttgagatgg tggagatcct ctcgacatta agtatggaca ttgacacgct gcgggcggcg
    33961 ctgctgttcc ctctggctga tgccaacgta gtcagcgaag atgtgctgcg tgagagcgtc
    34021 ggtaagtcgg ttgttaacct tattcacggc gtgcgtgata tggcggcgat ccgccagctg
    34081 aaagcgacgc acactgattc tgtttcctcc gaacaggtcg ataacgttcg ccggatgtta
    34141 ttggcgatgg tcgatgattt tcgctgcgta gtcatcaaac tggcggagcg tattgctcat
    34201 ctgcgcgaag taaaagatgc gccggaagat gaacgtgtac tggcggcaaa agagtgtacc
    34261 aacatctacg caccgctggc taaccgtctc ggaatcggac aactgaaatg ggaactggaa
    34321 gattactgct tccgttacct ccatccaacc gaatacaaac gaattgccaa actgctgcat
    34381 gaacggcgtc tcgaccgcga acactacatc gaagagttcg ttggtcatct gcgcgctgag
    34441 atgaaagctg aaggcgttaa agcggaagtg tatggtcgtc cgaaacacat ctacagcatc
    34501 tggcgtaaaa tgcagaaaaa gaacctcgcc tttgatgagc tgtttgatgt gcgtgcggta
    34561 cgtattgtcg ccgagcgttt acaggattgc tatgccgcac tggggatagt gcacactcac
    34621 tatcgccacc tgccggatga gtttgacgat tatgtcgcta acccgaaacc aaacggttat
    34681 cagtctattc ataccgtggt tctggggcca ggtggcaaaa ccgttgagat ccaaatccgc
    34741 accaaacaga tgcatgaaga tgcagagttg ggtgttgctg cgcactggaa atataaagag
    34801 ggcgcggcag ctggcggcgc acgttcggga catgaagacc ggattgcctg gctgcgtaaa
    34861 ctgattgcgt ggcaggaaga gatggctgat tccggcgaaa tgctcgacga agtacgcagc
    34921 caggtctttg acgaccgggt gtatgtcttt acgccgaaag gtgatgtcgt tgatttgcct
    34981 gcgggatcaa cgccgctgga cttcgcttac cacatccaca gtgatgtcgg acaccgctgc
    35041 atcggggcaa aaattggcgg gcgcattgtg ccgttcacct accagctgca gatgggcgac
    35101 cagattgaaa ttatcaccca gaaacagccg aaccccagcc gtgactggtt aaacccaaac
    35161 ctcggttacg tcacaaccag ccgtgggcgt tcgaaaattc acgcttggtt ccgtaaacag
    35221 gaccgtgaca aaaacattct ggctgggcgg caaatccttg acgacgagct ggaacatctg
    35281 gggatcagcc tgaaagaagc agaaaaacat ctgctgccgc gttacaactt taatgatgtc
    35341 gacgagttgc tggcggcgat tggtggcggg gatatccgtc tcaatcagat ggtgaacttc
    35401 ctgcaatcgc aatttaataa gccgagtgcc gaagagcagg acgccgccgc gctgaagcaa
    35461 cttcagcaaa aaagctacac gccgcaaaac cgcagtaaag ataacggtcg cgtggtagtc
    35521 gaaggtgttg gcaacctgat gcaccacatc gcgcgctgct gccagccgat tcctggagat
    35581 gagattgtcg gcttcattac ccaggggcgc ggtatttcag tacaccgcgc cgattgcgaa
    35641 caactggcgg aactgcgctc tcatgcgcca gaacgcattg ttgacgcggt atggggtgag
    35701 agctactccg ccggatattc gctggtggtc cgcgtggtgg ctaatgatcg tagtgggttg
    35761 ttacgtgata tcacgaccat tctcgccaac gagaaggtga acgtgcttgg cgttgccagc
    35821 cgtagcgaca caaaacagca actggcaacc atcgacatga ccattgagat ttacaacctg
    35881 caggtgctgg gccgcgtgct gggtaaactc aaccaggtgc cggatgttat cgacgcgcgt
    35941 cggttgcacg ggagttaggc agaaatttgc tcgtatctac aatgtagatt gatatatact
    36001 gtatctacat atgatagcgg tttgaggaaa gggttatgat ccacagtagc gtaaagcgtt
    36061 ggggaaattc accggcggtg cggatcccgg ctacgttaat gcaggcgctc aatctgaata
    36121 ttgatgatga agtgaagatt gacctggtgg atggcaaatt aattattgag ccagtgcgta
    36181 aagagcccgt atttacgctt gctgaactgg tcaacgacat cacgccggaa aacctccacg
    36241 agaatatcga ctggggagag ccgaaagata aggaagtctg gtaatggtaa gccgatacgt
    36301 acccgatatg ggcgatctga tttgggttga ttttgacccg acaaaaggta gcgagcaagc
    36361 tggacatcgt ccagctgttg tcctgagtcc tttcatgtac aacaacaaaa caggtatgtg
    36421 tctgtgtgtt ccttgtacaa cgcaatcaaa aggatatccg ttcgaagttg ttttatccgg
    36481 tcaggaacgt gatggcgtag cgttagctga tcaggtaaaa agtatcgcct ggcgggcaag
    36541 aggagcaacg aagaaaggaa cagttgcccc agaggaatta caactcatta aagccaaaat
    36601 taacgtactg attgggtagt gttactaact cacttctatt ctggtcacag gtttccacct
    36661 gacagacccg caattttcag gacaattcaa tgaatcaaat cgaccgtttg ctcactatta
    36721 tgcagcgcct gcgcgatccg gaaaacggct gcccgtggga taaagagcag acatttgcca
    36781 ccattgcgcc ttacaccctt gaagaaacct acgaagtgct ggacgccatc gcccgtgaag
    36841 attttgacga tcttcgcggt gaactgggcg atctgctatt ccaggtggtg ttttacgcgc
    36901 aaatggctca ggaagaaggg cgctttgact ttaatgatat ttgcgctgct attagcgata
    36961 aattagagcg tcgccatccg catgtttttg ctgatagttc tgccgaaaac agtagtgaag
    37021 tgcttgcccg ttgggagcaa atcaaaaccg aagagcgcgc gcagaaagcg cagcattcgg
    37081 cgctggacga tattcctcgt agtttaccgg ctttaatgcg ggcgcaaaaa atccagaaac
    37141 gttgcgccaa cgttggcttc gactggacga cgcttggccc ggtggttgat aaagtctacg
    37201 aagagatcga cgaggtgatg tacgaggcgc ggcaggctgt tgtcgaccag gctaaactgg
    37261 aggaggaaat gggggacctg ctgtttgcca cggttaatct ggctcgccat ttagggacga
    37321 aagcagaaat cgcattgcaa aaagcgaacg aaaaattcga gcgtcgtttt cgcgaagtgg
    37381 agcgtattgt tgccgcgcgt ggactggaaa tgacaggtgt tgacctcgaa acaatggaag
    37441 aagtctggca acaggtaaaa cggcaggaaa ttgatctcta agggaattac gcggtcaagc
    37501 gccatttgtg tcatttttta aatgacaagc gcttgatttg cgtcaaaaac atttacccca
    37561 aaggggctat tttctcactc ctgatttcaa tagtgcgctg gcgaagagga gggataatga
    37621 aagtttgtgg cacaggtcat gttcgggtat actgctttcc cgtcctggtt attccatcgt
    37681 cttttcaacc taacttctca ggttcagcat gacaacgaac tatatttttg tgaccggcgg
    37741 ggtcgtatcc tctctgggta aaggcattgc cgcagcctcc ctcgcagcca ttcttgaagc
    37801 ccgtggcctc aatgtgacca tcatgaaact ggatccgtac atcaacgtcg atccaggtac
    37861 tatgagccca atccaacacg gggaagtgtt cgttactgaa gacggcgctg aaaccgacct
    37921 ggacctgggg cactacgagc gtttcattcg taccaaaatg agccgccgca acaacttcac
    37981 cacgggtcgt atctactctg acgttctgcg taaagaacgc cgcggtgact acctcggcgc
    38041 aaccgtgcag gttattccgc acatcactaa cgcaatcaaa gagcgcgtgc tggaaggtgg
    38101 cgaaggtcat gacgtagtac tggtagaaat cggcggtaca gtaggtgata tcgaatcctt
    38161 gccgttcctc gaagcgattc gccagatggc tgttgaaatt ggccgtgagc acactctgtt
    38221 tatgcacctg acgctggtgc cgtacatggc agcgtctggt gaagtcaaaa ccaaaccgac
    38281 tcagcactct gtaaaagagc tgctctccat cggtatccag cctgacatcc tgatttgtcg
    38341 ttcagatcgc gctgttccgg cgaacgaacg tgcgaagatt gcattgttct gtaatgttcc
    38401 ggaaaaagcg gttatttctc tgaaagacgt cgattccatc tataaaattc cgggcctgtt
    38461 gaaatctcag gggctggacg attatatttg taaacgattc agcttaaact gcccggaagc
    38521 aaatctgtcc gaatgggaac aggttatctt cgaagaagcg aatccggtaa gtgaagtcac
    38581 catcggtatg gtcggcaagt acattgaact gccggatgct tataaatcag tgatcgaagc
    38641 actgaaacac ggtgggctga agaatcgtgt cagcgtcaac atcaaactga tcgattcaca
    38701 agatgttgaa acgcgcggcg ttgaaatcct taaaggtctg gacgcaatcc tcgtacctgg
    38761 cggtttcggc tatcgtggcg tagaaggcat gattacgacc gcgcgttttg cgcgtgagaa
    38821 caatattcct tatctgggca tttgcctggg tatgcaggtg gcgttaattg attacgctcg
    38881 ccatgttgcc aacatggaga acgccaactc tacggaattt gtgccagact gtaagtaccc
    38941 ggttgtggcg ctgattaccg agtggcgcga tgaaaacggc aacgttgaag ttcgtagcga
    39001 gaagagcgat ctcggcggta ccatgcgtct cggcgcacag cagtgccagt tggttgacga
    39061 tagcctggtt cgccagctgt acaatgcgcc gacaattgtt gagcgtcatc gtcaccgtta
    39121 cgaagtcaac aacatgctgt tgaaacagat tgaagatgca ggtctgcgcg ttgcgggccg
    39181 ttccggggat gatcagttgg tcgagatcat cgaagttccg aatcacccgt ggttcgtggc
    39241 ttgccagttc catccggagt ttacttctac tccacgtgat ggtcacccgc tgtttgcagg
    39301 ctttgtgaaa gctgccagcg agttccagaa acgtcaggcg aagtaagtaa aaaagttaga
    39361 acggcaacgc gtaccttggg tacgcgttgt ttgtctggag tttcagttta actagtgact
    39421 tgaggaaaac ctaatgtcca aaatcgtaaa aatcatcggt cgtgaaatca tcgactcccg
    39481 tggtaacccg actgttgaag ccgaagtaca tctggagggt ggtttcgtcg gtatggcagc
    39541 tgctccgtca ggtgcttcta ctggttcccg tgaagctctg gaactgcgcg atggcgacaa
    39601 atcccgtttc ctgggtaaag gcgtaaccaa agctgttgct gcggtaaacg gcccgatcgc
    39661 tcaggcactg attggcaaag acgctaaaga tcaggctggc attgacaaga tcatgatcga
    39721 cctggacggc accgaaaaca aatccaaatt cggcgcgaac gcaatcctgg ctgtatctct
    39781 ggctaacgcc aaagctgctg ctgccgataa agggatgccg ctgtacgagc acatcgctga
    39841 actgaacggc actccgggca aatactccat gccggttccg atgatgaaca tcatcaacgg
    39901 tggtgagcac gctgacaaca acgttgatat ccaggaattc atgattcagc cggttggcgc
    39961 gaaaactgtg aaagaagcca tccgcatggg ttctgaagtt ttccatcacc tggcaaaagt
    40021 tctgaaagcg aaaggcatga acactgcagt tggtgacgaa ggtggctatg cgccgaacct
    40081 gggttccaac gctgaagctc tggctgttat cgctgaagct gttaaagctg ctggttatga
    40141 actgggcaaa gacatcactc tggcgatgga ctgcgcagct tctgaattct acaaagacgg
    40201 taaatacgtt ctggctggcg aaggcaacaa agctttcacc tctgaagaat tcactcactt
    40261 cctggaagaa ctgaccaaac agtatccgat cgtatctatc gaagacggtc tggacgaatc
    40321 tgactgggac ggtttcgcat accagaccaa agttctgggc gacaaaatcc agctggttgg
    40381 tgacgacctg ttcgtaacca acaccaagat cctgaaagaa ggtatcgaaa aaggtatcgc
    40441 taactccatc ctgatcaaat tcaaccagat cggttctctg accgaaactc tggctgcaat
    40501 caagatggcg aaagatgctg gctacactgc agttatctct caccgttctg gcgaaactga
    40561 agacgctacc atcgctgacc tggctgttgg tactgctgca ggccagatca aaactggttc
    40621 tatgagccgt tctgaccgtg ttgctaaata caaccagctg attcgtatcg aagaagctct
    40681 gggcgaaaaa gcaccgtaca acggtcgtaa agagatcaaa ggccaagcat aagactgact
    40741 ttatctgatt taaaaa
//
LOCUS       DAJTVE010000018        40673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000018 DAJTVE010000000
VERSION     DAJTVE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..687
                     /locus_tag="OO439_000948"
     CDS             <1..687
                     /locus_tag="OO439_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCR6315379.1"
                     /translation="RREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYY
                     DPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMAPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
     gene            689..925
                     /locus_tag="OO439_000949"
     CDS             689..925
                     /locus_tag="OO439_000949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315380.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1098..1773)
                     /locus_tag="OO439_000950"
                     /pseudo
     CDS             complement(1098..1773)
                     /locus_tag="OO439_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1918..2526)
                     /locus_tag="OO439_000951"
     CDS             complement(1918..2526)
                     /locus_tag="OO439_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6315381.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OO439_000952"
     CDS             complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OO439_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6315382.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3304..4464
                     /gene="metB"
                     /locus_tag="OO439_000953"
     CDS             3304..4464
                     /gene="metB"
                     /locus_tag="OO439_000953"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6315383.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4467..6899
                     /gene="metL"
                     /locus_tag="OO439_000954"
     CDS             4467..6899
                     /gene="metL"
                     /locus_tag="OO439_000954"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6315384.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7248..8138
                     /gene="metF"
                     /locus_tag="OO439_000955"
     CDS             7248..8138
                     /gene="metF"
                     /locus_tag="OO439_000955"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6315385.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8467..10647
                     /gene="katG"
                     /locus_tag="OO439_000956"
     CDS             8467..10647
                     /gene="katG"
                     /locus_tag="OO439_000956"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6315386.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10741..11646
                     /gene="yijE"
                     /locus_tag="OO439_000957"
     CDS             10741..11646
                     /gene="yijE"
                     /locus_tag="OO439_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6315387.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11673..12290)
                     /locus_tag="OO439_000958"
     CDS             complement(11673..12290)
                     /locus_tag="OO439_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6315388.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OO439_000959"
     CDS             complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OO439_000959"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6315389.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OO439_000960"
     CDS             complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OO439_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6315390.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OO439_000961"
     CDS             complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OO439_000961"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6315391.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17162..18241
                     /locus_tag="OO439_000962"
     CDS             17162..18241
                     /locus_tag="OO439_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6315392.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18256..18576
                     /locus_tag="OO439_000963"
                     /pseudo
     CDS             18256..18576
                     /locus_tag="OO439_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18627..20923
                     /locus_tag="OO439_000964"
                     /pseudo
     CDS             18627..20923
                     /locus_tag="OO439_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20889..21767
                     /locus_tag="OO439_000965"
     CDS             20889..21767
                     /locus_tag="OO439_000965"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6315393.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21769..22110
                     /locus_tag="OO439_000966"
     CDS             21769..22110
                     /locus_tag="OO439_000966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6315394.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22097..22948)
                     /locus_tag="OO439_000967"
     CDS             complement(22097..22948)
                     /locus_tag="OO439_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6315395.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23163..24896)
                     /locus_tag="OO439_000968"
     CDS             complement(23163..24896)
                     /locus_tag="OO439_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6315396.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25079..27730)
                     /gene="ppc"
                     /locus_tag="OO439_000969"
     CDS             complement(25079..27730)
                     /gene="ppc"
                     /locus_tag="OO439_000969"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6315397.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28082..29233)
                     /gene="argE"
                     /locus_tag="OO439_000970"
     CDS             complement(28082..29233)
                     /gene="argE"
                     /locus_tag="OO439_000970"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6315398.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29387..30391
                     /gene="argC"
                     /locus_tag="OO439_000971"
     CDS             29387..30391
                     /gene="argC"
                     /locus_tag="OO439_000971"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6315399.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30399..31175
                     /gene="argB"
                     /locus_tag="OO439_000972"
     CDS             30399..31175
                     /gene="argB"
                     /locus_tag="OO439_000972"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6315400.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31236..32609
                     /gene="argH"
                     /locus_tag="OO439_000973"
     CDS             31236..32609
                     /gene="argH"
                     /locus_tag="OO439_000973"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6315401.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32876..33793
                     /gene="oxyR"
                     /locus_tag="OO439_000974"
     CDS             32876..33793
                     /gene="oxyR"
                     /locus_tag="OO439_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6315402.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33776..35176)
                     /gene="sthA"
                     /locus_tag="OO439_000975"
     CDS             complement(33776..35176)
                     /gene="sthA"
                     /locus_tag="OO439_000975"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6315403.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35497..36147
                     /gene="fabR"
                     /locus_tag="OO439_000976"
     CDS             35497..36147
                     /gene="fabR"
                     /locus_tag="OO439_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6315404.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36147..36506
                     /locus_tag="OO439_000977"
     CDS             36147..36506
                     /locus_tag="OO439_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6315405.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36546..37646)
                     /gene="trmA"
                     /locus_tag="OO439_000978"
     CDS             complement(36546..37646)
                     /gene="trmA"
                     /locus_tag="OO439_000978"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6315406.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37760..37950
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38015..39859
                     /gene="btuB"
                     /locus_tag="OO439_000979"
     CDS             38015..39859
                     /gene="btuB"
                     /locus_tag="OO439_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6315407.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39804..40661
                     /gene="murI"
                     /locus_tag="OO439_000980"
     CDS             39804..40661
                     /gene="murI"
                     /locus_tag="OO439_000980"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6315408.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9386 a        10126 c        11180 g         9981 t
ORIGIN      
        1 cgccgggaat atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag
       61 cagcttattc gtttgccggg gcagcagtat gatgaggagt ccggcctgta ttataaccgc
      121 caccgctatt atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag
      181 gggggatgga acctgtatac atatccgctg agcccggtga atagcatggc tccattagga
      241 ttatatgaat ttaaatcaaa aaatattgat gatattggaa tatttgcatt ggcaatgtgt
      301 aatggagaat caattaacga gaataaagaa tatggtggac taatatgtaa gaagcaaggt
      361 gaatatttcc ccatgaatcc gataagttca aatgataatg atagtgtaga cttgcgaaat
      421 ataaaatgcc ctgaaggttc agagagagta ggcgattatc acactcacgg tttttactct
      481 gacgataaag gaaataaagt aacaaaagaa aatgatgttt atgatagtct aaatttttca
      541 agcaaagatt taacgaattc ttatatgaat ggaatgggaa aaaaagaata cagtagttac
      601 ttgggaacac caaataacac ctatctaaaa tataatccca aagctaaagg gaatggagtt
      661 acaattatca ggcaagggag taattaaaat gaaggcaata caatgttgtt atttccttgc
      721 tttagtctcc agtcatttaa tggctggagt tatgggtgat gataatatat ttttaagtaa
      781 ttcggattca gctgctttat attgtgtggc taaaatattt ccagatggag aaaatatttt
      841 aatatataaa gtaaatataa ttaatgctag ttcaaaaaaa gatgtttttt ttatgaaagc
      901 ccaacatcaa cccacaatga cataatgggg ggcgggggaa tgtcatatgc ctatgaatat
      961 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
     1021 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
     1081 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
     1141 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
     1201 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
     1261 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
     1321 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
     1381 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
     1441 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
     1501 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
     1561 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
     1621 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
     1681 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg
     1741 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
     1801 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt
     1861 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
     1921 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     1981 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     2041 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     2101 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     2161 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     2221 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     2281 ttcaccatgg gcgatccact gcttgagcgg ggggtatttc accgggccga ccgcttcaaa
     2341 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     2401 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     2461 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     2521 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     2581 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     2641 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     2701 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     2761 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     2821 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     2881 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     2941 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     3001 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     3061 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     3121 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     3181 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     3241 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     3301 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     3361 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     3421 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     3481 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     3541 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     3601 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     3661 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     3721 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     3781 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     3841 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     3901 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     3961 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     4021 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     4081 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     4141 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     4201 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     4261 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     4321 atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc cgagacgctg
     4381 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     4441 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     4501 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     4561 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     4621 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     4681 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     4741 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     4801 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     4861 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     4921 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     4981 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     5041 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     5101 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     5161 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     5221 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     5281 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     5341 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     5401 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     5461 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     5521 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     5581 cgctgaaaat cctcgacgaa gcggggttac ctggcgaact gcgcctgcgt caggggctgg
     5641 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     5701 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     5761 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     5821 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     5881 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     5941 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     6001 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     6061 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     6121 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     6181 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     6241 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     6301 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     6361 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     6421 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     6481 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     6541 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     6601 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     6661 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     6721 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     6781 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     6841 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     6901 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     6961 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     7021 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     7081 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     7141 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     7201 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     7261 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     7321 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     7381 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     7441 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     7501 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     7561 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     7621 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     7681 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     7741 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     7801 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     7861 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     7921 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     7981 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     8041 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     8101 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     8161 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     8221 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     8281 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     8341 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     8401 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     8461 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     8521 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     8581 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     8641 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     8701 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     8761 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     8821 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     8881 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     8941 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     9001 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     9061 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     9121 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     9181 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     9241 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     9301 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     9361 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     9421 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     9481 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     9541 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     9601 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     9661 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     9721 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     9781 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     9841 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     9901 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
     9961 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
    10021 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
    10081 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
    10141 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
    10201 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
    10261 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
    10321 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
    10381 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
    10441 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
    10501 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
    10561 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
    10621 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
    10681 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
    10741 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
    10801 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
    10861 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
    10921 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    10981 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    11041 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    11101 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    11161 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    11221 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    11281 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    11341 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    11401 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    11461 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    11521 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    11581 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    11641 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    11701 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    11761 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    11821 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    11881 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    11941 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    12001 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    12061 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    12121 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    12181 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    12241 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    12301 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    12361 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    12421 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaaa aagaaaattc aatatattgc
    12481 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    12541 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    12601 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    12661 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    12721 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    12781 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    12841 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    12901 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    12961 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    13021 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    13081 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    13141 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    13201 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    13261 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    13321 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    13381 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    13441 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    13501 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    13561 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    13621 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    13681 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    13741 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    13801 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    13861 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    13921 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    13981 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    14041 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    14101 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    14161 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    14221 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    14281 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    14341 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    14401 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    14461 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    14521 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    14581 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    14641 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    14701 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    14761 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    14821 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    14881 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    14941 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    15001 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    15061 tcgagcgccc acaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    15121 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    15181 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    15241 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    15301 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    15361 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    15421 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    15481 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    15541 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    15601 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    15661 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    15721 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    15781 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    15841 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    15901 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    15961 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    16021 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    16081 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    16141 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    16201 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    16261 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    16321 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    16381 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    16441 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    16501 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    16561 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    16621 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    16681 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    16741 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    16801 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    16861 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    16921 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    16981 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    17041 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    17101 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    17161 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    17221 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    17281 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    17341 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    17401 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    17461 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    17521 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    17581 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    17641 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    17701 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    17761 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    17821 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    17881 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    17941 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    18001 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    18061 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    18121 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    18181 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    18241 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc
    18301 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    18361 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    18421 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    18481 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    18541 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    18601 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    18661 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    18721 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    18781 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    18841 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    18901 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    18961 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    19021 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    19081 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    19141 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    19201 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    19261 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    19321 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    19381 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    19441 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    19501 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    19561 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    19621 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    19681 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    19741 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    19801 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    19861 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    19921 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    19981 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    20041 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    20101 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    20161 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    20221 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    20281 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    20341 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    20401 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    20461 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    20521 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    20581 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    20641 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    20701 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    20761 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    20821 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    20881 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    20941 gcgatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    21001 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    21061 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    21121 aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata
    21181 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    21241 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    21301 ttttacagcg actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc
    21361 cagcaagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa
    21421 ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga
    21481 atctgcgttt gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg
    21541 gtttcacgct cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa
    21601 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    21661 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    21721 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    21781 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg
    21841 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga
    21901 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    21961 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    22021 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg
    22081 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    22141 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    22201 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    22261 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    22321 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    22381 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    22441 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    22501 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    22561 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    22621 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    22681 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    22741 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    22801 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    22861 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    22921 agagcagata gctgacgccg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    22981 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    23041 aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc
    23101 gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat
    23161 aattactgat tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt
    23221 ttcttgtacg ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa
    23281 cgggttggat cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt
    23341 tccgccaggc tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc
    23401 catttttccg acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct
    23461 tcgttacgcc cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag
    23521 aagtaaacca ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc
    23581 acatgatcgt tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg
    23641 cttaatcctg gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag
    23701 cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca
    23761 gggtcattca gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc
    23821 tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc
    23881 atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc
    23941 tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg
    24001 gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta
    24061 ttaaacacgg tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca
    24121 cgcggataac cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact
    24181 aaagtgcgcg gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta
    24241 ttttcattca gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc
    24301 aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca
    24361 aacggcttgt ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc
    24421 agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc
    24481 cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa
    24541 tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg
    24601 ctctgcgaga actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc
    24661 catagcacca cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc
    24721 gggatcaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa
    24781 ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc
    24841 caacccaggg ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta
    24901 agaacctgtc ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa
    24961 ggtatattca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga
    25021 acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt
    25081 agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga
    25141 cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg
    25201 cctgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc
    25261 acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt
    25321 cttcttgcag gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt
    25381 gatcatagta ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga
    25441 gacgcgtcga gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat
    25501 cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt
    25561 tttgcgtcca ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc
    25621 gacgtttcgc cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg
    25681 agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg
    25741 agatgactga cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca
    25801 gcaggttggc ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg
    25861 gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca
    25921 ggcttcctgg cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg
    25981 aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta
    26041 atgcatcctg tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg
    26101 agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga
    26161 tattgagcag ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca
    26221 gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca
    26281 gtacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg
    26341 cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt
    26401 tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg
    26461 cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt
    26521 cggtatggcg ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt
    26581 tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct
    26641 gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc
    26701 cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca
    26761 tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc
    26821 caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct
    26881 gaatatcttt caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg
    26941 tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac
    27001 ggaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca
    27061 gttcgcgcaa gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc
    27121 atttggcttc atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact
    27181 gggcgattaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt
    27241 tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac
    27301 gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt
    27361 ttttgatggt gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga
    27421 tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg
    27481 tgttggccag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg
    27541 acaaattttg taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag
    27601 atttcgacaa cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct
    27661 tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt
    27721 gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg
    27781 tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa
    27841 aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta
    27901 cgccattaaa ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc
    27961 atacataaca ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt
    28021 gcggcaccgg agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt
    28081 tttaatgcca gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa
    28141 accgcgtctc cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca
    28201 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    28261 tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    28321 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca
    28381 ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ctaggcagcg
    28441 gacgaatatc catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt
    28501 gaatatgccc gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt
    28561 ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag
    28621 cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt
    28681 tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg
    28741 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    28801 cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc
    28861 gtagcgtatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    28921 gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg
    28981 gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca
    29041 gcatattgaa tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat
    29101 ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    29161 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    29221 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    29281 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    29341 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    29401 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    29461 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    29521 ctccgatttg catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga
    29581 tatcagcgag tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag
    29641 ccacgattta gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc
    29701 gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc
    29761 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    29821 agcgaatttg attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc
    29881 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    29941 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    30001 accgtatggc gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga
    30061 cgttatcttc accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    30121 ccgcctgaaa tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc
    30181 ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    30241 gctgccattt tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac
    30301 cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt
    30361 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    30421 aactgggcgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg
    30481 tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    30541 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga
    30601 cgcctgctga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    30661 tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    30721 acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag
    30781 gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca
    30841 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc
    30901 tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg
    30961 gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    31021 gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc
    31081 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta
    31141 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    31201 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    31261 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    31321 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg
    31381 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gagcgtgctg ctggaagatg
    31441 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    31501 aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc
    31561 gtaacgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac
    31621 tgacggctaa ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg
    31681 cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt
    31741 gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc
    31801 gtctggatgt cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc
    31861 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg
    31921 tttctgaccg tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc
    31981 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    32041 tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    32101 tggagctgat tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga
    32161 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    32221 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    32281 ttcaggtgaa acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg
    32341 aactggcgga ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg
    32401 gtgaagcggt ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg
    32461 agttgcagaa attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat
    32521 cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga
    32581 ttgcttttgc gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc
    32641 ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac
    32701 gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa
    32761 aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc
    32821 gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa
    32881 tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc
    32941 agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga
    33001 gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat
    33061 gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc
    33121 aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg
    33181 accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat
    33241 gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga
    33301 ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga
    33361 tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc
    33421 gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga
    33481 tcaggcaatg ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac
    33541 cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc
    33601 gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc
    33661 ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta
    33721 tgagcagctg gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa
    33781 acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt
    33841 taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg
    33901 cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct
    33961 cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg
    34021 cgcgtgccag atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca
    34081 gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga
    34141 tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc
    34201 cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa
    34261 cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt
    34321 ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga
    34381 gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac
    34441 agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt
    34501 gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt
    34561 tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca
    34621 ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc
    34681 tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc
    34741 aggcaataac aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg
    34801 ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt
    34861 cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga
    34921 tatcggcaaa agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga
    34981 attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg
    35041 tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa
    35101 ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat
    35161 cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta
    35221 acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg
    35281 cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat
    35341 actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt
    35401 ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat
    35461 agtgccagca gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa
    35521 aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc
    35581 agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct
    35641 ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt
    35701 ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg
    35761 atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg
    35821 ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa
    35881 attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt
    35941 gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag
    36001 gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg
    36061 cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt
    36121 attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc
    36181 tgctggcgtt agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca
    36241 ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc
    36301 aacgttatct taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta
    36361 ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta
    36421 acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta
    36481 accgtaagca ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat
    36541 gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga
    36601 tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat
    36661 aacgtttccg ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg
    36721 gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc
    36781 tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat
    36841 tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat
    36901 tgcgcagcag caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc
    36961 gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag
    37021 cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg
    37081 gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg
    37141 tcgatgtaat cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca
    37201 ttcagattct gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca
    37261 tccagcttct tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa
    37321 tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc
    37381 gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt
    37441 ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac
    37501 tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa
    37561 aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt
    37621 tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt
    37681 gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg
    37741 gacagtaact tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat
    37801 ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc
    37861 ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc
    37921 cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc
    37981 tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac
    38041 ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt
    38101 cgttactgct aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt
    38161 gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct
    38221 tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg
    38281 cggtacaaat gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg
    38341 ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata
    38401 tatccgtggg ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat
    38461 catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta
    38521 tcagaactat gatgtctcta cgcagcaaca actgggggat aagacacggg gaacgctgtt
    38581 gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca
    38641 agcgcagaca gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa
    38701 ctttactgat gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta
    38761 tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag
    38821 ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta
    38881 tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac
    38941 gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg
    39001 tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt
    39061 tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg
    39121 cgattttacc tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg
    39181 aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta
    39241 cgggacatct tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa
    39301 tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt
    39361 gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca
    39421 caccctgaaa tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa
    39481 ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc
    39541 gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg
    39601 gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa
    39661 ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct
    39721 tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt
    39781 cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc
    39841 tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg
    39901 ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt
    39961 tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg
    40021 tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca
    40081 acactgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg
    40141 gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac
    40201 tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta
    40261 atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc
    40321 tacatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa
    40381 tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag
    40441 aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc
    40501 gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata
    40561 ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt
    40621 acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga atg
//
LOCUS       DAJTVE010000019        39867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000019 DAJTVE010000000
VERSION     DAJTVE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..147)
                     /locus_tag="OO439_000981"
     tRNA            complement(72..147)
                     /locus_tag="OO439_000981"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Lys,seq:ttt)
     gene            complement(181..256)
                     /locus_tag="OO439_000982"
     tRNA            complement(181..256)
                     /locus_tag="OO439_000982"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Lys,seq:ttt)
     gene            complement(305..380)
                     /locus_tag="OO439_000983"
     tRNA            complement(305..380)
                     /locus_tag="OO439_000983"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Lys,seq:ttt)
     gene            complement(384..459)
                     /locus_tag="OO439_000984"
     tRNA            complement(384..459)
                     /locus_tag="OO439_000984"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Val,seq:tac)
     gene            complement(609..684)
                     /locus_tag="OO439_000985"
     tRNA            complement(609..684)
                     /locus_tag="OO439_000985"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Lys,seq:ttt)
     gene            complement(849..1640)
                     /gene="cpoB"
                     /locus_tag="OO439_000986"
     CDS             complement(849..1640)
                     /gene="cpoB"
                     /locus_tag="OO439_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6315409.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1650..2171)
                     /gene="pal"
                     /locus_tag="OO439_000987"
     CDS             complement(1650..2171)
                     /gene="pal"
                     /locus_tag="OO439_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6315410.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2206..3498)
                     /gene="tolB"
                     /locus_tag="OO439_000988"
     CDS             complement(2206..3498)
                     /gene="tolB"
                     /locus_tag="OO439_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6315411.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3631..4863)
                     /gene="tolA"
                     /locus_tag="OO439_000989"
     CDS             complement(3631..4863)
                     /gene="tolA"
                     /locus_tag="OO439_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6315412.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4928..5356)
                     /gene="tolR"
                     /locus_tag="OO439_000990"
     CDS             complement(4928..5356)
                     /gene="tolR"
                     /locus_tag="OO439_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6315413.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5360..6052)
                     /gene="tolQ"
                     /locus_tag="OO439_000991"
     CDS             complement(5360..6052)
                     /gene="tolQ"
                     /locus_tag="OO439_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6315414.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6049..6453)
                     /gene="ybgC"
                     /locus_tag="OO439_000992"
     CDS             complement(6049..6453)
                     /gene="ybgC"
                     /locus_tag="OO439_000992"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6315415.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6603..6896)
                     /gene="ybgE"
                     /locus_tag="OO439_000993"
     CDS             complement(6603..6896)
                     /gene="ybgE"
                     /locus_tag="OO439_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6315416.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6896..7009)
                     /gene="cydX"
                     /locus_tag="OO439_000994"
     CDS             complement(6896..7009)
                     /gene="cydX"
                     /locus_tag="OO439_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6315417.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7024..8163)
                     /gene="cydB"
                     /locus_tag="OO439_000995"
     CDS             complement(7024..8163)
                     /gene="cydB"
                     /locus_tag="OO439_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6315418.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8179..9747)
                     /gene="cydA"
                     /locus_tag="OO439_000996"
     CDS             complement(8179..9747)
                     /gene="cydA"
                     /locus_tag="OO439_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6315419.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10343..10570)
                     /locus_tag="OO439_000997"
     CDS             complement(10343..10570)
                     /locus_tag="OO439_000997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315420.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10595..13151)
                     /locus_tag="OO439_000998"
                     /pseudo
     CDS             complement(10595..13151)
                     /locus_tag="OO439_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(13169..15085)
                     /gene="mngA"
                     /locus_tag="OO439_000999"
     CDS             complement(13169..15085)
                     /gene="mngA"
                     /locus_tag="OO439_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6315421.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15254..15976
                     /gene="mngR"
                     /locus_tag="OO439_001000"
     CDS             15254..15976
                     /gene="mngR"
                     /locus_tag="OO439_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6315422.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(16080..16949)
                     /gene="sucD"
                     /locus_tag="OO439_001001"
     CDS             complement(16080..16949)
                     /gene="sucD"
                     /locus_tag="OO439_001001"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6315423.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16949..18115)
                     /gene="sucC"
                     /locus_tag="OO439_001002"
     CDS             complement(16949..18115)
                     /gene="sucC"
                     /locus_tag="OO439_001002"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6315424.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18209..19426)
                     /gene="odhB"
                     /locus_tag="OO439_001003"
     CDS             complement(18209..19426)
                     /gene="odhB"
                     /locus_tag="OO439_001003"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6315425.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19441..22242)
                     /gene="sucA"
                     /locus_tag="OO439_001004"
     CDS             complement(19441..22242)
                     /gene="sucA"
                     /locus_tag="OO439_001004"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6315426.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22543..23259)
                     /gene="sdhB"
                     /locus_tag="OO439_001005"
     CDS             complement(22543..23259)
                     /gene="sdhB"
                     /locus_tag="OO439_001005"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6315427.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23275..25041)
                     /gene="sdhA"
                     /locus_tag="OO439_001006"
     CDS             complement(23275..25041)
                     /gene="sdhA"
                     /locus_tag="OO439_001006"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6315428.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(25041..25388)
                     /gene="sdhD"
                     /locus_tag="OO439_001007"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(25041..25388)
                     /gene="sdhD"
                     /locus_tag="OO439_001007"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6315429.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25382..25786)
                     /gene="sdhC"
                     /locus_tag="OO439_001008"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25382..25786)
                     /gene="sdhC"
                     /locus_tag="OO439_001008"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6315430.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26167..26274
                     /locus_tag="OO439_001009"
     CDS             26167..26274
                     /locus_tag="OO439_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315431.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26480..27763
                     /gene="gltA"
                     /locus_tag="OO439_001010"
     CDS             26480..27763
                     /gene="gltA"
                     /locus_tag="OO439_001010"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6315432.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28153..28719
                     /locus_tag="OO439_001011"
     CDS             28153..28719
                     /locus_tag="OO439_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6315433.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28770..31228
                     /locus_tag="OO439_001012"
                     /pseudo
     CDS             28770..31228
                     /locus_tag="OO439_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31243..31974
                     /locus_tag="OO439_001013"
     CDS             31243..31974
                     /locus_tag="OO439_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6315434.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31971..33032
                     /locus_tag="OO439_001014"
     CDS             31971..33032
                     /locus_tag="OO439_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6315435.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33184..34230
                     /locus_tag="OO439_001015"
     CDS             33184..34230
                     /locus_tag="OO439_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6315436.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34227..35018)
                     /gene="nei"
                     /locus_tag="OO439_001016"
     CDS             complement(34227..35018)
                     /gene="nei"
                     /locus_tag="OO439_001016"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6315437.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(35054..35788)
                     /gene="pxpA"
                     /locus_tag="OO439_001017"
     CDS             complement(35054..35788)
                     /gene="pxpA"
                     /locus_tag="OO439_001017"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6315438.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35778..36710)
                     /gene="pxpC"
                     /locus_tag="OO439_001018"
     CDS             complement(35778..36710)
                     /gene="pxpC"
                     /locus_tag="OO439_001018"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6315439.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36704..37360)
                     /gene="pxpB"
                     /locus_tag="OO439_001019"
     CDS             complement(36704..37360)
                     /gene="pxpB"
                     /locus_tag="OO439_001019"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6315440.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37383..38126)
                     /gene="ybgI"
                     /locus_tag="OO439_001020"
     CDS             complement(37383..38126)
                     /gene="ybgI"
                     /locus_tag="OO439_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6315441.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38397..>39867
                     /gene="dtpD"
                     /locus_tag="OO439_001021"
     CDS             38397..>39867
                     /gene="dtpD"
                     /locus_tag="OO439_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6315442.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALA"
BASE COUNT         9664 a        10713 c         9700 g         9790 t
ORIGIN      
        1 aacctgctgc aggttcgaat ctttgagtat cgcagatagc aaaaaagcgc ctttagggcg
       61 cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
      121 ctgctctacc aactgagcta acgacccctt gcgggattat gctgctttaa tcattcagga
      181 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
      241 actgagctaa cgacccatac gggtgttacc tgaaagattt ctactcgggg ccaatcttta
      301 agattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
      361 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
      421 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
      481 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggatga ctcgcttcgc
      541 tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttaacatc tgtcttcgat
      601 gctaaaactg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
      661 ctctaccaac tgagctaacg acccactttt tcgttgcttt cggtttgttt gatatcccgt
      721 ggcaacggcg gcatatatta ctgatttcag acttgagcgc aacaaaaatt tcgatgcaga
      781 tcactcaact gcttatgatt cgcacgacac gaccagaaat aatgcgactt ctggtcgtgt
      841 gttatgcatt acatcgcgtt cagacgtttt tgtgcctgtt tagcgccatc agtaccaggg
      901 tatttactga taacctgctg gtatacggct ttcgcttttg cggtgtcacc tttgtcctgc
      961 atgatgacgc cgactttaaa catcgcatca gcagcctttg gtgacttcgg atagtttttc
     1021 actaccgaag caaaatagta cgccgcatca tcttttttac ccttgttgta gtttaactga
     1081 cccagccaat aattggcgtt tggcaggtaa gttgaatcag ggtaattttt gatgaaattc
     1141 tgaaatgcca ccattgcgtc atcctggcgg gatttatcct gcaccagcgc aatagctgca
     1201 ttgtaatccg tgtttgcatc gccgcttttc accggcgcgc cagcattcgc agtaccagca
     1261 tcagctgtcg gcgtcgttga tgccgccgca ccgctttgat cgctgctggt tgattgcgcc
     1321 gctgcaccac cgctgctgag gctgtcgatc tgcaacagga tctgcttttg ccgctccacg
     1381 acctgattca gttgatactg attttcctga atctgaccac gcagggaatc aatatcggat
     1441 tgattatcag aaagttgttg ctggagttgg gttaaaagct ggctgtgagc attagaaata
     1501 cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc cgacactact gattggtgcc
     1561 tgagcaaaag cggcccaggg ggccgctata ccaaccagta acgacagact caatagttga
     1621 tgtctgaagt tactgctcat gcaattctct tagtaaacca gtaccgcacg acggtttttg
     1681 gagtatgccg cttcgtcatg acccagtact gcaggttttt ctttaccgta agaaacgatg
     1741 gagatctggt ctgcagaaac gcctttaccc tgcaggtaca tcttaacggc gttcgcacga
     1801 cgttcaccca gggagatgtt gtattccgga gtaccacgtt cgtccgcgtg accttctacg
     1861 gtgactttgt aagacgggtt gctacgcagg aagtttgcat gtgcatccag catttgagcg
     1921 aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa cgatgttgtt ctgctgcagc
     1981 tgttgcattt gcagacgagc ctgctcttcg gaagacatgt tgccgttgcc gccgttcgca
     2041 tccataccag tgccggcacc cagcatgcct tcgctgccgt cattgctggc gttcttgttg
     2101 gaagaacatg ccgcaattgc cataacaggc agagcaatca tcagcccttt cagcactttg
     2161 ttcagttgca tttcaatgat tcctttacta ttcaattaat tattatcaca gatacggcga
     2221 ccaggcaggg aatttgacct gtccatcagt tgccggaaga cgcgctttga aacgcccatc
     2281 tgtagaaacc aaattcagca cggatcccat cccctgagaa gagctgtaga ttaccatagt
     2341 gccgttaggt gccagacttg gcgtttcatc caggaacgtg gacgacagaa cttgtacgcc
     2401 tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca ccgttggagc tgaccattac
     2461 cataaattta ccgtcgctgc tgacatccgc atcctggttc tgcgaacctt cccaggtaat
     2521 acgttgtggc gcaccgccgt tgatattcac tttataaacc tgtggacgac cggcctggtc
     2581 agaagtaaat gccaggttct ggctgtccgg gaaccaggtc ggttcggtat tgttactgcg
     2641 accatcagtc acctggcgga tctgaccaga agccaaatcc attacgtaca ggttcaggct
     2701 accggttttc gacaaggcga atgccagttt gctgccgtct ggcgagaatg caggcgcacc
     2761 gttgtgacgc gggaatgaag ccacctgacg tacagcgccg tttgccagcg tttggataac
     2821 tagcgcggaa cgaccgcttt cgaaggtcac ataagccagt ttagaaccgt ctggtgacca
     2881 cgccggagac atcagcggct gtggtgaacg gtgaacgaca aactggttgt aaccgtcata
     2941 gtcagatacg cgcagttcat acgggaactg accgccgttg gtctgaacaa cgtaggcaat
     3001 acgggtacgg aacgcacctt taatgccggt cagtttttca aacacttcat cactggcggt
     3061 atgaccagca tagcgcagcc actgcttgtt cactttgtac gagttctgag caagtacagt
     3121 acccggtgcg ccgccagtgt caacaagttg ataagcaaca ttgtaagaac catccggatt
     3181 cggagtgacc tgaccgacaa ctacagcgtc aatacccagc gcggaccatg cagctggttg
     3241 tacttcctgc gcactacccg gctgctgtgg cagacgagcg cgatctaacg gattaaattt
     3301 accgctgtta cgcaagtcgg cagcaacgat gccgccaata tcttcaggtg ccgcaccagg
     3361 gcccgcccac tggaaaggaa caacaccaat aggacgaccg gaatctacac cgctgtcgat
     3421 cacaatgcgg acttcagcat gcagaactga tgcccacagt atgagaaaac caaatgctac
     3481 tcgtaatgcc tgcttcatca tatctccctt atctggaccg atggcccacg ataatttagc
     3541 agaatgttaa caaactaaaa tacacaaaac taccagaacc ccgtggcaac cggtgcctga
     3601 tgttgaccgt cggaacagtc aacatcgcga ttacggtttg aagtccaatg gcgcgttttt
     3661 gaacacttca tatactgcct ggcttggtgg tttcgggatc ttcgcaagtt tagctgctgc
     3721 caacgcagcc tgacaaagtg cgggatcgcc accttcaggt ttgatatcca gtaacatacc
     3781 atcgggtgcc agttttatgc gcagcgtaca ggttttgcct gcataggacg atgcgtcata
     3841 gaacttactt tcgatagcag atttaatctg cccggcatag ttattgatat cggcccctga
     3901 tgcgccattg tttttagtat taccactccc ggcagacgaa gcattgttcc ctttcgcccc
     3961 tccccccgtt ttcggtgcat tcttaccaga gcttagctca ccgaaaatat catctgcctc
     4021 tgcggcagct tttgctgcag cggctttttc agctgctgct tttgccgctg ccgctttttt
     4081 gtctgcggcg gctttttctg ccgctgcttt tgccgctgcc gcttttttgt ctgcggcggc
     4141 tttttctgcc gctgctttct tatcagctgc tgccttatca gcagccgctt tttctgctgc
     4201 ttttttgtcg gctgcagctt tctctgctgc cgctttcttt cttgcttcag ctgcagcagc
     4261 ttctgccgct tccgctttct tcttcagtgc cgcagcggct gcctcggctt ttttctgcgc
     4321 ttcggctgcg gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg ccgccgcttt
     4381 cttcgctgct tcttccgcag ctttagcatc cgcttcggcc ttcgctttag catctgccgc
     4441 cgctttcgct gccgcctctt ccgcttgctt ctgctttaac tcggcctgtt ttgcggcttc
     4501 ttcagcctgc tttttctgtt cctgagcagc taaccgctct ttctcaagtt gcttcaggcg
     4561 ttcctgttca gccgcttgtt tctcacgcag ttcttcagca gcctgctgtt cctttatctt
     4621 gcgctgctca tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt tgtactgctc
     4681 aactaccgca cctgaatcaa ccatgacagc gtcgatggac gaaccaccgc cgcctccggc
     4741 tgaagcttct atattctcat cgaacgaact ccagagcagc gccgcaaata agatgacatg
     4801 cagcactgct gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga
     4861 cactctcggt ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc aagggaaacg
     4921 cagatgttta gataggctgc gtcattaaac caaccgattt cacacccgca ctatgtaaca
     4981 agttcagtgc tttaattatt tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga
     5041 ccgttttcgg gttggccttg aaacggctgg acacttccgc caccacctgc tctggtggta
     5101 aacgctccag gcgatctttc tcaaccacca cggtgtactg accaatacca gacacttcaa
     5161 caatcactgg cggattatcg ttactgctca ccgcctgtga ttcagtagcg tctggcagat
     5221 cgacctccac gctctgggtg atgatgggcg ctgtcgccat aaagatcagc aacagcacca
     5281 gcagtacgtc cagcaacggt acaatgttga tttcggactt gagatcgcga cgacctcgtc
     5341 cacgcgctct ggccatggct taccccttgt tgctctcgct aacggtaaac gcctggcggt
     5401 gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt cagttccagt ttgtttacgc
     5461 gctggttgag gcggttataa gccattaccg ccggaattgc ggcgaacaga ccaattgcag
     5521 tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg cagtgttgct tgttttaccg
     5581 ccccgagggc gataaaggcg tgcatgatcc cccagaccgt accaaacaga ccaatatacg
     5641 ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac
     5701 ggttcatgga gatacgcata gcacgcgacg ccccttccac tacggcttcc ggcgcatggc
     5761 tattggcacg atgcaggcgc acaaactctt tgaacccgct gtaaaatatt tgttccgaac
     5821 cagtcagatt atcccgtttc ccctgactct cttgatagag gcgagagagt tcgattccag
     5881 accagaattt atcttcaaac gcttcggctt cgcgcgccgc tgcgttaaga atacgggtcc
     5941 gctggataat aatggcccag gatgcgattg aaaaaccaat caaaatcaac atgataagtt
     6001 taaccagaag gctagccttc aggaacaaat caaggatatt catgtcagtc actgcttaaa
     6061 ctccgcgaca atagacttgg gaagcgcaca aggcttcatt ttgagtgggt caacgcaaac
     6121 aaccagaacc tctgcttcat tcagcaaagt attctcggcg ttgacaatac gttgcgtgaa
     6181 aaccaaagag gtgccacgca ttgatgttat ttcagtctgt atttcgagca tatcgtcgag
     6241 ccgcgcaggt acgtaatatt ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc
     6301 catcagcgcc tgctgactga agtgatgatg acgcagcatc tctgtgcgtg ctctttcata
     6361 aaaagcgacg taactggcgt ggtacaccac accaccggca tcggtatctt catagtagac
     6421 gcgaaccggc catcgaaaca gcgttgtatt cactttacat cccggtaatg caacaaaagt
     6481 tagagctttt aaacgctgct actatacgcg cgcgattgag gtttgggaat gagggcaagt
     6541 taagggagcg taaaaattta tgggcctgga gcggcccata attttttaaa gaaaattctg
     6601 atttaaaaga agaagaaaat cagcccgaca atgagaacaa tatcggcaag caatgggcaa
     6661 aaaatccctt gccaaagaac cttctgcgga cgaaagccca cgccgtgaat cacaccagca
     6721 cagacggccc acatcagcaa taaaccatgc cagatttcca gttcactggt cttcgcggca
     6781 aaacgagatg ggtcccaaaa catacatcct gctaacagaa gcgccatcac gaaggaaagc
     6841 gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg cgataatctt actcatcaga
     6901 tgtcttcttg accggctttg cctgattcga cgtgttcaag cgccagcgcg gtgattaccc
     6961 caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa ataccacatt tttagctcct
     7021 tacttagtac agagagtggg tgttacgttc aatatcttct ttggtgatac gaccgaacat
     7081 tttccagtaa caccaagcgg tgtagagcag aatgatcggt accagaacca ccgcaaccca
     7141 ggtcatgacg ttaagcgtca gctggctgga agttgcatcc cacattgtca gacttgcgtt
     7201 catcatggtg ctggacggca tcacaaacgg gaacattgcg ataccggctg tcaggatgat
     7261 gcaggccagc gtcagggagg agaacacaaa cgcccacgcg gctttatcca tacgtgcagt
     7321 caggatggtc agcagcggaa gaaccacacc cagtgccgga atagcccaca gaattggcgt
     7381 gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc acttctttat tcagtgggtt
     7441 agaggctgcg taatggtcca ttgtcgattt cacgacataa ccatcgatac cgtacatcac
     7501 ccatacgcca gccagtgcga aacagaccag tgtcaccagc gcagccacct gagccgttgc
     7561 acgggtacgc aggtgcagtt cgcccacggt acgcatttgc agataggttg cgccctgagt
     7621 aatgatcatc cctacgctca ccacgcctgc cagcaggccg aacgggttaa gcaactggaa
     7681 gaagttaccg gtgtagtaca gacgcagata ttcatcaacg ttgaacggta cgccctgcaa
     7741 caggttaccg aacgctacac caattaccag cggcggaacg aaactaccaa tgaagatgcc
     7801 ccagtcccac atgttacgcc agcgggtttc ttcaatcttg gagcggtagt caaaaccgac
     7861 cggacggaag aacaaagacg ccagcacgag gatcatcgcc acatagaagc cggagaacgc
     7921 agcggcatag accatcggcc aggcagcaaa gagtgcgccg cccgcggtga tcagccaaac
     7981 ctggttaccg tcccagtgtg gtgcaatgga gttaatcata attcgacgct cggtgtcgtt
     8041 acgaccgagg aaacgggtga gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa
     8101 accaatcagc agaacgccaa ccagcagcca ccagataaaa cgcaatactt cataatcgat
     8161 catttgacga ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg
     8221 atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca aacttgaaca ttaagaacaa
     8281 ttctgccacc aggaacaggg tatacaggcc gcaaatcagc accattgaga agatgagatc
     8341 gcctgcggtc agtgacgagt tcgccacagc tgtcggcagc acttcaccga tagcccacgg
     8401 ttggcggcca tattcagcca cgaaccagcc cgcttctaca gcaatccacg gcagcggaat
     8461 accgtacagc gcggcgcgca gaagccattt tttctcgcca atgcggttgc ggatgacact
     8521 ccagaaagag agcgcgatga ttgccagaag caggaagcca cacgccacca tgatacggaa
     8581 cgcaaagtac agcggcgcta cacgcgggat ggagtctttg gttgcctgtt gaatctgcgc
     8641 ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc agcagaccgt aaccgaggtc
     8701 tttcttcatg ctattgaact ggtcacgaac cgcctggtcg gtagaaccag aacgcagttg
     8761 ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag
     8821 ctctttcagg ccgataaccg gggtatccac ggaacgcgtt gcaatgatgc ccagtgcgta
     8881 agggatctga atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc cgaacagagt
     8941 aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca atagcagcca gtttggtttt
     9001 ctgcacgtcg cccatttcgt agccggattc atcacccaga acaataacag acagaacagc
     9061 agccataccg aagctggcag cgatagcaaa ggagcgttta gcgaaggcga agtcacgacc
     9121 tttcagcata taccatgcgc tgataccgag gatgaacatc gcgccagtca cataaccaga
     9181 cgctacagtg tgaacgaatt tcacctgagc aaccgggtta agcaccagct cggagaagct
     9241 caccatctcc atacgcatag tttcaaagtt gaaatcggac gcgattgggt tttgcatcca
     9301 gccgttcgca accagaatcc acagtgcgga caggtttgaa ccgagcgcca ccagccaggt
     9361 gacacacata tgctgaactt tacccagacg atcccaaccg aagaagaaca gacctacaaa
     9421 ggtggattcg aggaagaagg ccatcagacc ttcgattgcc agcggcgcac cgaagatatc
     9481 ccctacatag tgggaatagt aagaccagtt agtcccgaac tggaactcca tggtcagacc
     9541 ggtagccaca cccagagcga agttgatacc aaacaacttg ccccagaact tggtcatatc
     9601 tttataaatc tgtttgccgg agaggacgta gaccgtttcc ataatggcca gcaggaacgc
     9661 cataccgagc gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa
     9721 gcgcgacagt tcgactatat ctaacatcat gactccttgc tcatcgcatg aagactccga
     9781 gagtgaaccc cgttagaaag ggtcacacgc atgccccaat agaaatttgt ttgaacactt
     9841 tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga aaggttgcaa acatgttaat
     9901 aaaaactcaa attgatccca cgtatatatt acgctgcaaa atccttacaa taaacaggtt
     9961 tttgttgaag caaatttgca tttttcgacg gtgatcaatt tatagctaaa ttaccgcctt
    10021 tcagccaatt tgatcgagaa caatttatct ctttttgatg ccaatttcca agacttatac
    10081 attgataaat atcaattcct acaaattatg ttaatcatgt ttcaataagg taactttagt
    10141 gacatttatg tttaaaatgt gtgagttata aggctgaaat tagtttcggt tggcaattta
    10201 aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt tttggtcact
    10261 ggctaatacg acgcagagtt tcaggattac taataaaggc ataaatccac cccgccgata
    10321 ttaacattta attaacttgt aattatatta taattaactc acccagggtt attattttgt
    10381 taaaaataaa gccacatata cgtggcttga gagtgaagat aaacgcaaaa ttacctgatg
    10441 cgctacgctt gccagtgtct acatcatctc tgcaatatat tgaatttgcc tgcttttgta
    10501 gaccgaataa agcgttcatg ccgtatccgg catgaacaaa gcgccctttg tcaacaatct
    10561 ggccgcgcat atgtggcttc ggtttgattg ctattcaggc aagccggtaa ctgaacgtcc
    10621 gtgactggcc gggtaaaaaa ggccccgata gatttgaacc ttgattttcc tcggtggtaa
    10681 tgtgttcatc catcatcgtt tctgagcaag aaatcacctc gcgactgaaa gcaacagtcg
    10741 catcacaggt tgctgattca gccggattaa acagccgtaa aatcacttct tgtcggtctt
    10801 cagctttctt aagtgcgctt atcaggcatc ccactggggg cattttcaac aaactataac
    10861 tttccggcac gttgaatccg gctttgttga gcttcatcgc atcccatggg attttgttgt
    10921 aacactgtac tggagtcagc catgctcgcg cctgctgagc tacaccagcg gcggttggcg
    10981 taccggtata actcaataaa cttaggcgac aagaaagcag accacgtagt tgtgagtccg
    11041 ggactggcat tttaattccc gaaggccgcc caggccttaa aagcagatct tctttgccca
    11101 gtaagcccac gccacgcagc aacgtaatgg caaaggtttt cttctcttca ccgatgactt
    11161 caaattcacg taacccttcg ctaaagacag ccatgccgtt acgcccttcc tgtaaggcaa
    11221 catagttgag catattccat accggaaccg gcgcttcttt ccagccttct tgctgccagt
    11281 tgttcattgc actgtcgttc acggggcgcg ttagcgaacc aaactgcgta tctgccagaa
    11341 cactgtcagt gttaaaaggt gtagggacca ggacacgaag gcgatgatcg tcagcctggt
    11401 tatcaaggtt gatatccaca tcaatacgcc tgctgttatg actaagagtg acaaccaaca
    11461 ccccccctac tctgccaggc ttcatgaata atatcgcatt gcggtttcgc gttcgctgcg
    11521 gtaattaccc actcttcttt tgcgggtgaa tagtcatatt catcaccatc atcagagctt
    11581 tcttcaattt gcaatacccg gtcatagcgc acaccgctgt ctttatctac cagttgcaga
    11641 gaaccatcct cattgagcgc aatctgccag aaagcatttt ccagaatccc ttcagcgtca
    11701 ctttttgccg caatcacgtt gccaggctga ttcgcttcaa tataaagcgt gcgatagccc
    11761 atagaaggga caatctggtt gatctgaata tcaaactcca taaagggatc ataattgcca
    11821 taatgaacaa tttgccgatc gattaggcca ggatcgatct cacgggcatg gcgaataaaa
    11881 tacggtacag gctgaccgcg atcgtcttgt agattaaact ggctggcgcg cagccgcaca
    11941 gtggtgttaa taacttcttc ccgcggccag ggcatcaggt taaacagcac gagtttgtcg
    12001 gcatcactct gtggcatgtt atcggcaatt ttgcgcatgt agaaacgcag cagattatcc
    12061 gccatgtctt cagccagttc gaagcgggcg acaatttcgc gatgaacttt gtcactgcaa
    12121 caacaaccga tactgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc
    12181 aagccgtggt ggtattcaaa acccaacgtc caggccagcg ttgccagcgg ttccagcaga
    12241 ttaacaatct tattttcaat acgtgcgtgg gcaattttga tatccatgcg cgtggaaccg
    12301 atggtgcgat gtacgcgcat atatttgcca tcgataaatt cacctttcag ggtggccaga
    12361 ctttcacgct gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaactta
    12421 cgttgagggt agatctcacg tagcttatcc atcacttcga agatattttg ctgcaatggc
    12481 atctggtcat gaccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg
    12541 tcaaaataac tgtcgaggcg tttacgtaat ccgttttcgt cggcaggtaa gtacttaccg
    12601 atggcgtagc ccagcggcag cacctgcgcc gtcacttcgc taccgtcact gctttgccac
    12661 aaaaactcgg ttttatcagt accgtggcgc tccgaacatc cgcgccagaa catagtgcgg
    12721 gtaatgccaa atccattgta aatatgcggc agttgcccgg acatgccaaa ggaatccggt
    12781 aaataaccta ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga
    12841 ttacggacga tggattccgc agaaacaatc gtggtatcgg tctgggtata ccaggggccg
    12901 ataatcaact taccggcttc tacctgtttc ttcacgcggt ctttgttttc cggtttcacc
    12961 gcgaaataat cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg
    13021 tcctgttcca gtcggcacag gatctcttcc atattattga ccagcagaat gcgtgactct
    13081 tcggtggtga aataccactc ccgatcccaa tgcatatgcg gggtgatgtg aacgcgagat
    13141 accgctttca tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc
    13201 catgcttaac cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca
    13261 ccaatgccgc accaaaccag ccaattgccg ccataacacc gcccgcgcca ttatcatgaa
    13321 gtaaaaagag cgagaaaatg ccggcaccgg gtgtcgaaag gccgatattc atcgcaccga
    13381 caatagcgcc cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt
    13441 caatcgccat cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt
    13501 tcccggtctc aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta
    13561 cggtaaatgc cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt
    13621 tcgccattgc gcccaggcag aatgcataag cggctttgtt cactggccct ccaaggtcaa
    13681 aggaacacat aaaaccgaga atggcaccca gcaacagcgc gttacttcct gacagaccgt
    13741 tcagccaggc ggtaagcgag ttattgatcc atgcgacagg ttctcccacc acaaacagca
    13801 tcagactgcc cgctcccaac gtaccgagca ccgggtagag ataaaaagtc aggaatccat
    13861 tgaatttact gctaagacgc aagtgatttt tcacccagcg catcaagtaa ccggctatca
    13921 atccgccaac gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg
    13981 caaagcctgg cgctaacgcc ggtttatctg ccagagaata ggtggtatag gccgcgagca
    14041 ccggtaccat caaaattccg agcagcccgc cgcccagctt gcggtacatc cacagccagg
    14101 agttttcttc attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg
    14161 ccagcaccgt gccccccgcg acaatcaacg gtacggcaaa agagattccg ctcaacagtg
    14221 cctgtttcag ctccgttttg acacttttca ccggttgcgt atcttgctgt acggtacgcg
    14281 tctcatcgct acgcttgagg gtaagcgctt gttggatcaa cgcttctgca tggcgaatcg
    14341 gctcggcaac aggcactgaa agcgcgggaa tgccattaaa acgctcactc tccttgatgg
    14401 cgacttcagc cgcaaaaata caggcggttg cactattgag ttgatccgcc gttaaacgcc
    14461 cttcaatgcc gttagcgcct tgtttttcaa cgtaaacatt tacgccgagt ttgcgtccgg
    14521 ctttttccag atattccgca gccatatagg tgtgagcaat acccgccgga caggccgtta
    14581 cgcagacgat agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat
    14641 ccagcgccga gagcaactca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat
    14701 catccgcaag gcgcgtggtc agcgctgtca gcagttgcat atgcgtagta cccgcttcat
    14761 tggggggaat cgccagcagc accactaaat caactgcttc cgggccatca acgccttccc
    14821 actgaagcgg ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt
    14881 tgccatgcgg cacagccaac ccttcaccta aggcggtcgg gccaaggctt tcacggcgat
    14941 acacttcttc cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag
    15001 tcaacgcgtg gatggcctct tcacggctgg taaagcgcgc attcagacac aacgcatcgc
    15061 ggtgggtcag agtcgtcaga ttcatcatga tcctcacttg atcgttttta tagtcgcgcc
    15121 agtgtgcccg ataaaacaat accatcatgt gatcaatttc acttgattga aattatttgt
    15181 attgtatttg tattatttac aggatgaatt tatcgggcat aatggctaaa ttcatattaa
    15241 tgagatacta aaaatgggac acaagccctt ataccggcag attgccgatc gcattcgtga
    15301 acaaattgcg cgtggcgagc ttaaacctgg cgatgcgtta cccactgaat cagcgctaca
    15361 aacagagttt ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagc tagtcgagca
    15421 gcagatcctc gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta
    15481 cgatattttt cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca
    15541 cagtgaaatc ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca
    15601 aatcaccgcg caggatcgtg tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc
    15661 aatggcgctg gaagaaacct ggatgccgct tgcgttgttc ccggatctca cctggcaggt
    15721 catggaaaat tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag
    15781 cgaacaggaa atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag
    15841 ccagacaaag ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt
    15901 tgaatatagc cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg
    15961 aaaatcatcg cgataagcac aaaaaaggcc atcatacgat ggccaaccat gtcgaaaccg
    16021 acagcccttc gccattcctg tagagggaag cggcgagggc tatttcttat tacagatatt
    16081 tatttcagaa cagttttcag tgcttcaccg atatccgcca ggctgcgaac ggttttcacg
    16141 cctgcggctt ccagagcagc gaatttctca tccgcagtcc ctttcccacc agcaatgatc
    16201 gcacccgcgt ggcccatacg tttgcctttc ggcgcagtca caccagcgat gtaacctaca
    16261 actggcttgg taacgtgctc tttgatgtac gctgccgctt cttcttcagc gctaccgccg
    16321 atctcaccga tcatcacgat cgcttcggtc tgcggatctt tttcgaacat ttcgaggatg
    16381 tcgataaagt tagagcctgg gatcgggtca ccgccgatac caacacaggt cgactgaccg
    16441 aaaccgtaat ccgtggtctg tttaaccgct tcataggtca gtgtaccgga acgggaaacg
    16501 atacccactt tacccggttt gtgaatgtga cccggctgga taccgatttt gcattcaccc
    16561 ggagtgataa cgcccgggca gttcgggccg atcatacgaa cgccggcttc gtccagtttc
    16621 actttcacag tcagcatatc cagcgtcggg atgccttcag tgatggtgat aatcagtttg
    16681 atgcctgcat caatagcttc cagaatggag tctttgcaga acggtgctgg tacgtagata
    16741 acagaagcgg tagcgccagt agcagcaaca gcttcacgca cggtgttgaa caccggcagg
    16801 ccgaggtggg tagtaccgcc tttacctggg gttacgccgc caaccatttt agtgccgtat
    16861 gcaatggcct gttctgagtg gaaagtcccc tggctaccag taaagccctg gcagataacc
    16921 ttggtgtttt tatcaattaa aatggacatt atttcccctc cactgcggca acaacctgct
    16981 gagctgcatc cgtcagacct tttgctgcaa taatattcag gccgctgtca gccagtttct
    17041 tcgcgccgag ttcggcgttg ttaccttcca gacgtaccac gaccggtacg ttaacaccca
    17101 cttctgctac cgcgccgatg ataccgtcag cgatcaggtc gcaacgaacg ataccgccga
    17161 agatgttaac cagaacggct ttcactttgt cgtcagagag gatgattttg aacgcttcgg
    17221 ttacacgttc tttggttgcg ccgccgccaa cgtcaaggaa gttagccggt tcgccgccgt
    17281 gcagtttaac gatgtccatc gtacccatcg ccagacctgc gccgttaacc atacaaccga
    17341 tgttaccgtc cagcgcaacg tagttcagtt cccactgtgc agcctgtgct tcacgcggat
    17401 cttcctgcga ctggtcacgc atttcgcgca gatcaggctg gcggaacagt gcgttgccgt
    17461 cagcgcccag tttgccgtcg aggcaaatca gatcgccctg tttggtgatg accagcgggt
    17521 tgatttcgat caacgccagg tcgcgctcca ggaaaatggt cgccaggccc atgaagattt
    17581 tggtgaactg ctgaaccagt ttaccttcca gacccagttt gaacgccagc tcgcgtccct
    17641 gatacggcat cgggccagtc agcggatcaa gcgcaacttt gtggatcagg tgcggagttt
    17701 cttccgccac tttttcgatt tccacgccgc cttcggtgga ggccataaag accacacgac
    17761 gggaactacg gtctacaacg gcaccgagat acagctcttt agcgatatcg gtcgctgctt
    17821 caaccagaat ctggttaacc ggttggccat tggcatctgt ttgatacgtt accagacgct
    17881 tgccgagcca gttttctgca aaagcacgga tgtcttcttt gctgtttaca actttcacac
    17941 cgcccgcttt accgcggcca ccagcgtgaa cctgacattt cactacccac gggccggcac
    18001 cgatttttga agcggcttct tctgcttcgc gcggagtagt acaggcataa cccaccggtg
    18061 ctggtaagcc atagcgggca aaaagttgtt ttgcctgata ttcatgtaag ttcatgtgtt
    18121 ctgtccatcc ttcagtaatc gttatctttt aaaccgtaga ccgacaccct atccacgtcg
    18181 gtctacagtg caggtgaagc ttaaactact acacgtccag cagcagacgc gtcggatctt
    18241 ccagcaactc tttgatcgtt accaggaagc ccacggattc gcgaccatcg atcagacggt
    18301 gatcgtagga cagcgccagg tacatcatcg gcaggatctc aacctgacca ttcaccgcca
    18361 tcggacgatc tttgatagcg tgcataccca gaattgcgct ctgcggcggg ttgatgatcg
    18421 gcgtagacat cagggaaccg aacacaccac cgttggtgat ggtgaagtta ccaccggtca
    18481 gatcttcaac cgtcagcttg ccgtcacggc ctttaactgc cagctctttg attttcttct
    18541 cgatgtctgc catgccgagg gtatcgacat cacgcagtac cggcgtcacc aggccgcgcg
    18601 gcgtggaaac cgccatgctg acgtcgaaat agttgtggta aaccacgtca tcaccgtcga
    18661 tagaagcatt cacttccggg taacgtttca gggcttcaac caccgctttc acgtagaagg
    18721 acataaagcc cagacggatg ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca
    18781 gatccataat cggcttcatg ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt
    18841 tcgcttccag cagacgctct gccacacgct tacgcaggcg agtcatcggg acacgttttt
    18901 cactacgtgc agccagagcc ggttgcgccg ccggagcagc cgctgccgga gcagactctt
    18961 tcgccggggc tttcgccaga tgtttttcca catcttcacg agtcagacga ccacccacac
    19021 cggtgccttt aatggcgctg gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg
    19081 ggcttaacgc atcgttgttt tgctcttcca gagacgcctg ctggcgttgc gccggagtgg
    19141 acgctttctc ttcagatttg gcgctggttt ctttaccggc gctgttgcct tcacgcaggc
    19201 gaccaaggat ctgacgagac gttaccgttg taccttcatc ttccagaacc gcatccagaa
    19261 tgccgtctgc tgatgccggt acttccagta ccactttgtc agtttcgatt tctaccagca
    19321 cttcatcacg tacgactgcg tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg
    19381 ctacggattc aggcaggtca gggaccagaa tatctacgct actcattgtg tatcctttat
    19441 ttattcgacg ttcagcgcgt cattaaccag atcttgttgc tgtttctggt gaacggacat
    19501 ataccctacc gccggagagg cggaggccgt gcggcctgca taacgcagag aagccccaaa
    19561 cggaatcact tcacggaaat gatgctggct gcagtaccat gcgccctggt tgagcggctc
    19621 ttcctggcac cagacaaaat ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc
    19681 tttatgcggg aacgggtaga gttgctcgat acgcacaatg gcgacatcgt gttgattgtt
    19741 cttacgacgc tgttccagca ggtcgtaata aaccttacca gaacacatca ctacgcgctt
    19801 cacgcccttc ggatcaagct cgtcgatttc accgatggct ggcaggaagg tgccgttcgc
    19861 cagttcttcg aggctggaaa ccgccagcgg atgacgcagc agggatttcg gcgacatcac
    19921 taccagcgga cgacgcatcc cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc
    19981 cggggtagac ggaacgcaaa cctgcatgtt ttgctcagca caaagttgca gataacgttc
    20041 cagacgcgcg gaggagtgct ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac
    20101 cagaccacac atccggcccc atttctgttc gccagaagag atgaactggt cgataaccac
    20161 ctgcgcaccg ttggcgaagt caccgaactg cgcttcccag atggtcagag tgcgtggttc
    20221 tgcggtggca taaccgtatt caaacgccag cactgcttct tcagacagta cggagtccca
    20281 gacgcggaac gcgccctgcc cgttatggat gtgttgcaac ggcgtgtaag tggaaccgtt
    20341 agactggttg tggatcaccg cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc
    20401 cgacaggcga accggaatgc cttcgtcaac cagcgtggcg taagcgaggt tttccgcacc
    20461 gccccagtcg aacagtttct caccggcagc catcgcctgg cgatcgccat aaatcttggc
    20521 aacgcgagac tgcatttcaa ctgcttccgg caccgtgctg atgcgtttcg ccagctcctg
    20581 caggcgcttc atctcaactt tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg
    20641 cgaccaggtg aaagagtgca tgttcatcgg acgccactct gccactacgc aatcgccagc
    20701 atccagcgca tcgcggtaca ggttaaccat ctcggtggca tcttccagcg tcgccacttt
    20761 ttcctgctcc agcttgtcag cgtagatttt gcgcggtgtc ggatgttttt tgattttctg
    20821 atacatcagc ggctgggttg cgctcggctc gtcggcttcg ttgtggccgt gacggcggta
    20881 gcacaccagg tcgatgaaga catcacgttt aaaggtgtta cggaaatcga gcgccagacg
    20941 ggtcacaaag gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac
    21001 catcttaccg atatcagtac agtacggtgt agaacgagca tccagtggat tagaggtggt
    21061 gaaaccaacc tggttgttga taacgatacg taccgtaccg ccaacttcat aaccacgcgc
    21121 tttcgacatg ttcagagttt cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg
    21181 gatggtgatt ggcagcactt tgttgctgct cggctcatca agtctgtcca gacgggcacg
    21241 aacagaaccg ataactaccg ggcttacaat ctcaaggtga gacgggttaa acgccagcgc
    21301 caggtgcacc aggccgccat cggtctggaa gtcagacgag aagcccatgt ggtatttcac
    21361 gtcacccgtg ccgaggtgtt ctttatgttt accggcaaac tcgtcgaaca agtcttgcgg
    21421 ttttttaccc agcacgttca ccagcacgtt cagacgacca cggtgcgcca tcccaagaac
    21481 cacttcgcgg gtgccgctgt tgccagcgtg gcggatcatc tctttaagca tcgggattaa
    21541 cgcgtcaccg ccttccagcg agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg
    21601 ttcaaggcct tcagcggcgg tcagttcgct taagaagcgt tttttctctt cgctattgaa
    21661 agtcgcgcga ccagactcga tacgctgttg gatccagcgt ttttcttcgg tgctggtaat
    21721 gtgcatatac tcggcaccaa tcgggccaca gtaggtttgc ttgagggctt ccagcagctc
    21781 gccgagtttc atgttttctt tgccgctggc aaatgaaccg acgttgaagg tctcctggaa
    21841 gtctgcttcg gtcagatcgt ggaaagacgg atccagatcg gccactttat cttgctgcca
    21901 cagtcccagc ggatcgagat tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag
    21961 ctgcaggact ttaacctgct tcacattggt gtcagggtcg gagatcgttg aagagtaacg
    22021 tgaagcgtct ttcgccaggc ggcggaaata ttcacgcgtt tgagagtgga attgatccgg
    22081 tttgactccc gtaccaggta actgctggaa cgtcgaacgc cagttagcgt caaccgagtc
    22141 aggatcggtt aagaagtctt catagagctg ttctatccag ctctggtttg cgccagagag
    22201 gtaagaagag tccaaccagg ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc
    22261 ttttttatgc ttacttcgcc gtggatacta ccacgcacag tgatgtgcgt gccggggttc
    22321 acttgcgagc tcttgtctat gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg
    22381 tcaggggcag tttttaaaga tttcctgcat ttctttgtta cgcctgatgc gctgcgctta
    22441 tcaggcctac gggtaatgac ttgtaggccg gataaggcgt tcacgccgca tccggcactg
    22501 gttgcctgat gcgacgcttg cgcgtcttat caggcctacg gtttacgcat tacgttgcaa
    22561 caacatcgac ttaatatggc cgatggcgcg cgtcgggttc agccccttcg gacatacact
    22621 gacgcagttc atgatgctgt gacagcggaa tacgctgaat gcatcgctca aaccgtcgag
    22681 gcggctgtca gtctcggtat cacggctatc gatcaggaaa cgatatgccg ctaacaagcc
    22741 tgccgggccg ataaacttat cgggattcca ccagaaagac ggacaagagg ttgaacaaca
    22801 tgcgcagaga atacattcat acagcccgtc gagtttttcg cgctgctctg gcatctgtaa
    22861 atgctcgcga gctggcggat tttgtccatt attcaacagg taaggcttaa ttttctcata
    22921 ttgcgcatag aattgtccca tgtctaccac caaatcgcgg atcaccggta aacctggcag
    22981 cgggcgaatc acaatcttct tgcccggctg gttgagtgcc gaaatcgggg taatacaggc
    23041 cagaccattc ttgccgttca tgttcagacc gtcggaaccg cacacacctt cacggcagga
    23101 gcggcggaac gacaggctgg gatctttctc tttcagctgg ataagcgcat ccagcagcat
    23161 catgtcgcga ccttcttccg cttccagggg gtaatcctgc atacgcggag catcatcaac
    23221 atccgggtta tagcgataaa ttgaaaactc gagtctcatt ttcctgtctc cgcattagta
    23281 agtacgaatc ttcggcggga atgccgggcg cagtttcggt tccatgttga cgcttcggcg
    23341 cgtcatggat tccgactctg gcagatacag ggagtggcac agccagtttt catcatcacg
    23401 atccgggaag tcgaagcggc tatgtgcgcc acggctttcg gtacggaagt tggcagaaac
    23461 agccgttgca tacgccgttt ccatcaggtt atccagttcc aagcactcaa cgcgctgggt
    23521 gttgaactcg ctggaagtgt catccagacg ggcatttttc agacgctcgc ggatcacttt
    23581 caactgctca agccctttcg ccatcgcatc accttcacgg aagaccgaga agttatgctg
    23641 catacattct tgcagcgctt tacgaatcgc caccggatct tcaccgttac gattattgtt
    23701 ccagcggtgc agacgatcca gagatgcttc aacatcagac tcgctggcat cgcgcaatgc
    23761 gccctgttcg gcgatagact cttgcagatg cagacctgcc gcacgaccaa agaccaccag
    23821 gtccagcagc gagttgccgc ccagacggtt agcgccgtgt accgatacac aagcgatttc
    23881 accaacggca aacagccccg gaacaaccac atcttcgcct ttctcgttca ccgtcagcgc
    23941 ctgaccggtc actttggtcg gaataccgcc catcatgtag tgacaggttg ggataaccgg
    24001 aatcggctct ttcaccggat cgacgtgagc aaaggtacgg gaaagctcca ggatacccgg
    24061 cagacgggat tcgagaactt ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc
    24121 ccacggacca tcacagccgc gaccttcacg gatttcaatc atgatggaac gcgccaccac
    24181 atcacggccc gccaggtctt tggcgttcgg cgcataacgc tccataaagc gttcgccatg
    24241 tttgttcagc agataaccgc cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc
    24301 ggcaatgccg gtcgggtgga attgccacat ttccatatcc tgcaccggta cgcctgcgcg
    24361 gatagccatg ccgacaccgt cgccagtgtt aatgtgggcg ttagtggtgg actgataaat
    24421 acgccctgcc ccgccagtcg ccagcacggt agcgcgggct ttgaaataaa ccacttcacc
    24481 ggtttcgatg cacagtgcgg tacaacccac caccgcgcca tcctggtttt tcaccagatc
    24541 cagcgcatac cactcggaga aaatggtggt gtggtttttc aggttctgct gataaagcgt
    24601 gtgcaacagt gcgtgaccgg tacggtcagc cgccgccgca gtgcgtgccg cctgctcgcc
    24661 gccgaagttt ttcgactgac cgccaaacgg acgttgatag atacgaccat catcaagacg
    24721 cgagaacggc aggcccatgt gttcgagttc cagaatcgct tccggcccgg ttttacacat
    24781 atattcaatc gcgtcctggt caccgatata gtccgaccct tttacggtgt cgtacatatg
    24841 ccattcccag ttatcttcat gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga
    24901 aacggtatgg gaacgggtcg ggaagacttt agagagcagc gcacaggtct ggccgctctg
    24961 ggaaatttgc agcgccgcgc gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa
    25021 ttctctgact ggcaatttca tcacacaccc cacaccacaa cgaatccata aatcacgtaa
    25081 accaccagtg caacgacaat caccagttgc agcatcaggc gcaatgccag cggtttaacg
    25141 tagtcggtca acacctgcca catgccgatc caggcatgaa tcaagataga aaacagcgcc
    25201 agcagggtga acactttggt gaacgcagag gcgaagaaac cgatccagac ttcatatgtc
    25261 agttcgccac tggtggcgaa aaaaccgacc atataaatga tgtagagcgt caggacgata
    25321 gcggtagcgc gaacgaggat gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg
    25381 cttaccatac gaggactcct gcgagaagtg aaagcacgac agtaataaca aaggagattt
    25441 tggcggagcg tttacccgct tcgaatgttt cttccagata gccaaaatcc atcatcatgt
    25501 ggcgaatacc tacgacgacg tgatacgcca gagcggtaag gatgccccac atgataaatt
    25561 tgacgaagaa gctgcccata atcgcggaag cttgctcgaa accttcaggg gaagagaggc
    25621 tggtacccag aagccacagc aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc
    25681 gatggagaat ggacgctatc gccgtgatgg ggaaccggat ggtctgtagg tccagattaa
    25741 caggtctttg ttttttcaca tttcttatca tgaataacgc ccacatgctg ttcttattat
    25801 tccctgggga ctacgggcac agaggttaac tttctgttac ctggagacgt cgggatttcc
    25861 ttcctccggt ctgcttgcgg gtcagacagc gtcctttcta taactgcgcg tcatgcaaaa
    25921 cactgcttcc agatgcgaaa acgacacgtt acaacgctgg gtggctcggg attgcagggt
    25981 gttccggaga cctggcggca gtataggctg ttcacaaaat cattacaatt aacctacata
    26041 tagtttgtcg ggttttatcc tgaacagtga tccaggtcac gataacaaca tttatttaat
    26101 ttttaatcat ctaatttgac aatcattcaa caaagttgtt acaaacattg ccaggaaaag
    26161 catataatgc gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt
    26221 gtggaagtat tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc
    26281 ggattgctaa gtacttgatt cgccatttat tcgtcatcaa tggatccttt acctgcaagc
    26341 gcccagagct ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa
    26401 cgggtaaagc caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgcaataa
    26461 ggcgctaagg agaccttaaa tggctgatac aaaagcaaaa ctcaccctca acggggacac
    26521 agctgttgaa ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac
    26581 tctcggttca aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga
    26641 atctaaaatt acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat
    26701 cgatcagctg gcgaccgatt ctaactacct ggaagtttgt tacatcctgc tgaatggtga
    26761 aaaaccgact caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat
    26821 ccacgagcag attacccgtc tgttccatgc tttccgtcgc gactcgcatc caatggcagt
    26881 catgtgtggt attaccggcg cgctggcggc gttctatcac gactcgctgg atgttaacaa
    26941 tcctcgtcac cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc
    27001 gatgtgttac aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta
    27061 tgccggtaac ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc
    27121 gattctggaa cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc
    27181 ctctacctcc accgtgcgta ccgctggctc ttcgggtgcg aacccgtttg cctgtatcgc
    27241 agcaggtatt gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa
    27301 aatgctggaa gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga
    27361 taaaaatgat tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc
    27421 gcgcgccacc gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga
    27481 tgacctgctg gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat
    27541 cgagaagaaa ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg
    27601 tattccgtct tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc
    27661 ccactggagc gaaatgcaca gtgacggtat gaagattgcc cgtccgcgtc agctgtatac
    27721 aggatatgaa aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaagtt
    27781 gtaaatattt taacccgccg ttcaaatggc gggttaattt ttatatgccc aaacacaaaa
    27841 aattgtaaaa ataaaatcca ctatcggaca ttcatatatt ctaaaaaaaa tatatcggta
    27901 taaattataa acaaatcgat gatttttaat catctatctt cctctcagtt acatgaataa
    27961 accaccaaat atagcgcacc cctataattt ccaatacttt attcatcaaa catttaattc
    28021 ataccatcaa ttaattactt ttacatttaa taaaattaat aatattttta agaaaaacta
    28081 aaaacattcg acaatcaaat aagatattct tgcaccgata tacttttttc ttttctgtta
    28141 aaggactcag ttatgtttaa aggacaaaaa acattggccg cactggccgt atctctgctg
    28201 ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg aaattcactt taaaggtgaa
    28261 gttattgaag caccgtgtga aatacatcag gatgatattg ataaagaggt tgaactcggt
    28321 caggtgacca ccagccacat taatcagtca catcacagcg atgccgttgc tgtcgacctg
    28381 cgcttagtca actgtgatct ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa
    28441 gttgcagtca cctttgatag ctcagcgaaa accaccggcg cagatccgat tctcaacaac
    28501 accagcacgg gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat
    28561 atcgttttag gcaccgctac tccagatatc gacctggctc cgacctccag cgaacaaacg
    28621 ctgaatttct ttgcctggat ggaacaaatt gatcaggcta cacctgtaac gccaggcgca
    28681 gttacggcga atgcaaccta cgtgctggat tataaataag tattactttg gcggcgctat
    28741 cgccgccttt cataaaatat ttatcaggta tggacaccgt gaatatttat cgactctctt
    28801 ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc aaccttaatg
    28861 ttctcgataa atcgatgcgc gaccgcattg atatttcatt attaaaggaa aaaggagtca
    28921 ttgctcccgg tgagtatttt gttagcgttg cggtaaataa taaccaaatc agtaacgggc
    28981 aaaagattaa ctggcacaaa aatgacgata aaaccattcc gtgcatcaat gatttactgg
    29041 tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt accattgata aatcagtgcg
    29101 tcgattttag ctcccgacct gaaatgctct tcaatttcga tcaagccaat cagcaactga
    29161 atattagtat tccgcaagcc tggctggcgt ggcactcaga aaactggacc ccaccctcca
    29221 catggaaaga aggtgtcgcc ggtatcctga tggattacaa cttgtttgcc agcagctacc
    29281 gcccacagga cggcagcagc agcactaacc tgaacgccta cggtaccacc ggaattaacg
    29341 ccggggcatg gcgcttacgt agtgattatc agttgaatca gactgatagc gatgataacc
    29401 atgaacagtc aggcggaata tcgcgcacct atctttttcg tccattaccg caattaggct
    29461 ctaaattaac cctcggcgaa acggatttta gttccaatat tttcgacggt ttttcttata
    29521 ccggcgcggc actggcaagt gacgagcgaa tgttgccatg ggaactacgc ggctacgccc
    29581 cacaaattag cggtattgca cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg
    29641 tcatttacca gaaaaaagtc ccaccaggcc catttatcat tgacgacctt aatcagtctg
    29701 ttcagggcac actggatgtc aaagtgacgg aagaagatgg tcgggtgaac aatttccagg
    29761 tttcggcagc atcgacgccc ttcctgactc gtcagggaca ggttcgctat aaactggccg
    29821 cgggtcagcc acggccctcc atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg
    29881 aagtttcctg ggggatgctg tcaaacacct cgctgtacgg cggcctgctg ctttctggtg
    29941 atgactacca ttctgccgca atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt
    30001 cgtttgatgt cacgtgggcc agtagccatt ttgatactca gcaggacgag cggggcttaa
    30061 gctaccgttt taattacagc aaacaagtgg atgccactaa cagcacgatt tccctcgccg
    30121 cttatcgttt ctccgatcgt cattttcaca gctacgccaa ctatctggat cacaaataca
    30181 acgacagcga tgcgcaggac gaaaaacaga cgatcagctt attgtgggcc aaccgattac
    30241 cccactaaac ctcaatcttt acgccaacct gctacatcaa acctggtgga atgcagacgc
    30301 ctccacgacc gccaacatca cggcaggttt taatgttgat attggtgact ggagagatat
    30361 ctctatttcg acgtcattca atacaaccca ttacgaagat aaagatcacg acaaccagat
    30421 ttacctgtcg atttcgctcc ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa
    30481 cagtagccac agcaccacac accgcatgtc gtggaacgat acgctggatg aacgtaatag
    30541 ctggggcatg tctgccggac tgcaatccga ccgtcctgac aatggagccc aggtgagcgg
    30601 taactatcag cacctgagtt cagcgggtga gtgggatatt tctggtacct atgccgccaa
    30661 tgattacagt tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc
    30721 agcgtttcat cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt
    30781 ggcagatatt ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt
    30841 gccgttgatt tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga
    30901 cggcgtaaca gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta
    30961 caaatcactg gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg
    31021 tcagtttcct cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat
    31081 ggttggcgag gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt
    31141 ggtctggggt gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc
    31201 aaatcgcctg attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga
    31261 ctaccccttt tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc
    31321 actcgtattg tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc
    31381 gataaactcc cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat
    31441 gatcttctgg tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacgcaggta
    31501 cggatagtaa agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc
    31561 tacaatatgc gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa
    31621 gtcgccattc agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca
    31681 ggagaaaagg tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa
    31741 aatcctaccg cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc
    31801 cccggcttca aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat
    31861 tatagcggca gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg
    31921 acgctcaact gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg
    31981 gcaagggagt gttgctcgtc atctgtctgt tatttctgcc gttgaagtct gcgcttgccc
    32041 tgaactgcta ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt
    32101 ttgctgtacc aggcaatgcc aaactcggcg ataagatttg ggaatctgac gatattaaaa
    32161 ttcccgtcta ttgtgacaac aataccaacg gcaattttga aagtgagcat gtttatgcct
    32221 gggtaaaccc ctacccgggg gtgcaggatc gctattatca actcggcgta acttacaatg
    32281 gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag tgtattgaca
    32341 gtaagaacat tgacatttat acccctgagc agattatcgc gatgggatgg cagaacaaaa
    32401 tttgctcggg tgatcccgct aatattcaca tgtcgcgtac ctttctcgct cgcatgcggt
    32461 tatacgtcaa aatacgagaa atgccgccgc atgattatca aagtacgctt agcgactata
    32521 tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga ccctactgcc caaaacctga
    32581 aatatcatat tactggtctg gaaaacattc gcgtgctgga ttgcagcgtc aatttttcca
    32641 tttcaccgga aacacaggtg attgattttg gtaaatttaa tttgctggat attcgtcgcc
    32701 acacaatgtc gaaaacattc agtattaaga cgacaaaatc acaaaatgac caatgcaccg
    32761 acggatttaa agtcagttcc tccttttata ccgaagaaac gttggttgaa gaagataaag
    32821 cattgttgat tggtaatggt ctgaagctac gtttgctgga tgaaaacgcc tcgccttata
    32881 ccttcaataa atatgctgaa tacgccgatt tcaccagcga catgttggtc tacgaaaaaa
    32941 cctatacagc tgaactttcg tccattgcag gtacccccat cgaggctggg cccttcgata
    33001 cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt gcagccggaa
    33061 agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct gttatttttt
    33121 cctggcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat gagggagcga
    33181 aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct ttctattggt
    33241 ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc cgggatcatc
    33301 ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc cgcgcaggcc
    33361 attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac gctggccgcc
    33421 aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc catcgtgggt
    33481 tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg ttcttctcca
    33541 ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat tcgcctggtt
    33601 gcatttatgc aatatctgcg agtgctgttc gtcgcggggg ccgctgttct ggtcacccgc
    33661 atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt cccgccagtg
    33721 agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc gggatgcatg
    33781 ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt gctccagtct
    33841 ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta tatggcaatt
    33901 ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact acgcccgcta
    33961 ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcgggtat ggcgtggggg
    34021 ctgacccggt ttatgcacat tgattttatg actgcctacc tcgccaccag tcccggcggg
    34081 cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct tattatggcg
    34141 atgcaaaccc tgcgcctgtt cagtattttg ctgacggggc cagccattgc gcggtttatt
    34201 tcaacctatg cgccgaagcg gtcggcctag tgctggcagc cagggcacca gtaaaacggg
    34261 cgagatgaca gcgtggtttt ctcaatgatg ctgccacagc gttcgcacgg ttcgccatca
    34321 cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc caccagcccc
    34381 ctcgtagcgt aggaaaatcg aggaatatcc agtaacgcgt gtgcgagtgc atccagttgc
    34441 gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca gaggatctcc
    34501 acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa tcctgcaaac
    34561 tgacggttac gaaagcgcgg tgacaataac cgttccttta ccacctccgg cgtcagattc
    34621 ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag ttgttccggc
    34681 ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc cgtttgcagt
    34741 tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac gcgccagacg
    34801 ccgtaaagct gattatggct gtagagcgtt aagtcgttgg aaaaatgagt taacaacgcc
    34861 ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg ataagttttt
    34921 aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc cgcctccagg
    34981 ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt ttttcattct
    35041 ttattaatcg gggttatgct gcgacaacaa tctccttttc ggcaaatgta gagcgtagtc
    35101 ggcgggcgaa cgccagtgcg tgctcgccgt cgccatgcag gcagactgtt tgcgccgtga
    35161 ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc tctagcgttt
    35221 gcgccaacgc ctgttcttcg ttttcaatca acgcgcctcg ctgacttcgc ggcaccagcg
    35281 agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc aggccatatt
    35341 gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaatcaat gccggatcgc
    35401 aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc gcctggttgt
    35461 acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg gcaatcgttg
    35521 ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttctggcggc agctgcatgg
    35581 cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc gcgacaccat
    35641 tttttattgc ttcacgcacg caagcctgca tgatttgcgc atcgcctgca tgaaatccac
    35701 aggcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg cagccttcgc
    35761 ccagatcggc gttcaggtca attttcattg tgcagccgcc acactaattg ttcgaaataa
    35821 cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac aaaatgaatc
    35881 ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat acaggcaata
    35941 cgcgggtaac catcggtggt ctgtgcgtcg ttcatcaaca caatcggctg cccgttatgt
    36001 ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc ggtggtgcgt
    36061 tttaaaattt gcccctgtaa gcgatagccc atgcggttac tttgcgagct aagctgccag
    36121 ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc cggccccggc
    36181 aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc cataaaatca
    36241 cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc ttccagcccg
    36301 ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac atcaatacca
    36361 cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt taaacgctgg
    36421 cctgctttca tcggcaatcg ccagccggtc cagacggcat tatcatccag ccgcgcttcg
    36481 caaccggcac ccgtcagagc aaaccaccca tcagtttcga actcaaccgt taactgaccg
    36541 agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt agcaatgcgt
    36601 aacgcaggca tatccagtgc gccgcagtgg ctgattcccg actggcgaaa accgtgacga
    36661 ccgccatcct gcacagtggt atacatgccc gcacgaataa tcttcagcat actccctcct
    36721 tctgcggtac aaagcgcacg ctgtctcccg gacgtaataa gatgggttcg tcacgcgccg
    36781 gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc gttgccagcg
    36841 gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg agcaagcgcg
    36901 gttcagcgcg ccgtggcgtg tgtagttgtt ccggcaaact cccgagatac gggaagcccg
    36961 gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca acaacctgtt
    37021 tttcgctaaa cccgcaatgc gccgcgacca ccgccaaatc cggtccgcct gcaccaccgt
    37081 aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc tcctcccacc
    37141 agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc aaaatcaccg
    37201 tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc agacgctgcg
    37261 ccagtcgcca gatccgtttc tggctagcca gcgtcaccgg cggttccagt tccagcacta
    37321 ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc tctgattatt
    37381 cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt ttcattcagc
    37441 cactcgctca atgcgcgaat accaccacgt tcagtggcat ggtgacctgc agcataaaaa
    37501 tgcaacccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc agtaataaaa
    37561 gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc cgtgcaccag
    37621 gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt acgtcccaga
    37681 cgcgcttcaa tccaggaggc tagttccagt cccggcacag gcatggtcag ctctccccac
    37741 ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc cagctgtgcg
    37801 ttattgccca gctcaggatg tacgtcaagc ggcaaatgcc agccatacag gttgatatca
    37861 ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg agactctcct
    37921 ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac tgcctcatcg
    37981 agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc tttgccttct
    38041 acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt ttcgttaatc
    38101 agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata gctacaccgt
    38161 taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat taagaaacaa
    38221 acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat gtatatttat
    38281 gcaatcagac agcatattgc ataaataatc agatctgtat caaagtttta actttaagga
    38341 aacacaagca cgccgcgcaa ctattacgtg acgttgcagt caattgtgga attattatga
    38401 ataaacacgc atcacaaccg cgcactattt actatgtcgt tgcgctgcaa atctgggaat
    38461 attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat caactaaaat
    38521 acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg tatgtcacgc
    38581 caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg gtgatgctgg
    38641 gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc catccgtcat
    38701 tcctctatct gtccctggcg attatcgttt gtggctatgg tctgtttaaa tccaatgtca
    38761 gttgcctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc ggtttctcgc
    38821 tgatgtatgc agcgggtaac gtggggtcta ttatcgcacc tatcgcctgt ggttacgccc
    38881 aggaagagta cagctgggcg atgggctttg gcctggcggc ggtgggtatg atcgcgggtc
    38941 tggtcatttt cttatgtggc aatcgtcatt tcacacatac ccgcggcgtt aataaaaaag
    39001 tactgcgtgc aacaaacttt ctcctgccta actggggatg gctgctggtt ctgctggtgg
    39061 caacgcctgc gctgattacc gtactgttct ggaaagagtg gtcggtatac gccttaattg
    39121 tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca gaaaaccaga
    39181 aacagcgtaa ggagctgggg ctgattgtga cgctcacctt cttcagtatg ttgttctggg
    39241 ccttcgcaca acagggcggc agctcaatta gcctttatat cgaccgcttc gttaaccgcg
    39301 atatgtttgg ttataccgtt ccgactgcta tgttccagtc gattaatgcc ttcgcagtta
    39361 tgctatgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt aatcgtaccg
    39421 tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga ttctgcattc
    39481 tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca ctgatggtgt
    39541 taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc atgtcgcaaa
    39601 ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac atgctgcttt
    39661 ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg caggcttcgt
    39721 ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta tttgatcaaa
    39781 ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg tatcaggcgc
    39841 tgaaattcag aaaccgcgcg ctggcgc
//
LOCUS       DAJTVE010000020        39809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000020 DAJTVE010000000
VERSION     DAJTVE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..438
                     /locus_tag="OO439_001022"
     CDS             7..438
                     /locus_tag="OO439_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6315443.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            521..1423
                     /locus_tag="OO439_001023"
     CDS             521..1423
                     /locus_tag="OO439_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6315444.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1478..3640)
                     /gene="uvrD"
                     /locus_tag="OO439_001024"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1478..3640)
                     /gene="uvrD"
                     /locus_tag="OO439_001024"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6315445.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3724..4440)
                     /gene="yigB"
                     /locus_tag="OO439_001025"
     CDS             complement(3724..4440)
                     /gene="yigB"
                     /locus_tag="OO439_001025"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6315446.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4440..5336)
                     /gene="xerC"
                     /locus_tag="OO439_001026"
     CDS             complement(4440..5336)
                     /gene="xerC"
                     /locus_tag="OO439_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6315447.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5333..6040)
                     /locus_tag="OO439_001027"
     CDS             complement(5333..6040)
                     /locus_tag="OO439_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6315448.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6037..6861)
                     /gene="dapF"
                     /locus_tag="OO439_001028"
     CDS             complement(6037..6861)
                     /gene="dapF"
                     /locus_tag="OO439_001028"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6315449.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6898..7101)
                     /locus_tag="OO439_001029"
     CDS             complement(6898..7101)
                     /locus_tag="OO439_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6315450.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7641..7961
                     /gene="cyaY"
                     /locus_tag="OO439_001030"
     CDS             7641..7961
                     /gene="cyaY"
                     /locus_tag="OO439_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6315451.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8001..10547)
                     /gene="cyaA"
                     /locus_tag="OO439_001031"
     CDS             complement(8001..10547)
                     /gene="cyaA"
                     /locus_tag="OO439_001031"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6315452.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10934..11875
                     /gene="hemC"
                     /locus_tag="OO439_001032"
     CDS             10934..11875
                     /gene="hemC"
                     /locus_tag="OO439_001032"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6315453.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11872..12612
                     /gene="hemD"
                     /locus_tag="OO439_001033"
     CDS             11872..12612
                     /gene="hemD"
                     /locus_tag="OO439_001033"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6315454.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12634..13845
                     /gene="hemX"
                     /locus_tag="OO439_001034"
     CDS             12634..13845
                     /gene="hemX"
                     /locus_tag="OO439_001034"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6315455.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            13848..15044
                     /gene="hemY"
                     /locus_tag="OO439_001035"
     CDS             13848..15044
                     /gene="hemY"
                     /locus_tag="OO439_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6315456.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15706..15867
                     /locus_tag="OO439_001036"
     CDS             15706..15867
                     /locus_tag="OO439_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315457.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15951..17186)
                     /locus_tag="OO439_001037"
     CDS             complement(15951..17186)
                     /locus_tag="OO439_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6315458.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17333..17409)
                     /locus_tag="OO439_001038"
     tRNA            complement(17333..17409)
                     /locus_tag="OO439_001038"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17373..17375),aa:Pro,seq:tgg)
     gene            complement(17452..17538)
                     /locus_tag="OO439_001039"
     tRNA            complement(17452..17538)
                     /locus_tag="OO439_001039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17502..17504),aa:Leu,seq:cag)
     gene            complement(17559..17634)
                     /locus_tag="OO439_001040"
     tRNA            complement(17559..17634)
                     /locus_tag="OO439_001040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17599..17601),aa:His,seq:gtg)
     gene            complement(17693..17769)
                     /locus_tag="OO439_001041"
     tRNA            complement(17693..17769)
                     /locus_tag="OO439_001041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17733..17735),aa:Arg,seq:ccg)
     gene            complement(17870..19255)
                     /locus_tag="OO439_001042"
     CDS             complement(17870..19255)
                     /locus_tag="OO439_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6315459.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19446..20186)
                     /gene="rffM"
                     /locus_tag="OO439_001043"
     CDS             complement(19446..20186)
                     /gene="rffM"
                     /locus_tag="OO439_001043"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6315460.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20189..21541)
                     /gene="wzyE"
                     /locus_tag="OO439_001044"
     CDS             complement(20189..21541)
                     /gene="wzyE"
                     /locus_tag="OO439_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6315461.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21538..22617)
                     /gene="rffT"
                     /locus_tag="OO439_001045"
                     /gene_synonym="wecF"
     CDS             complement(21538..22617)
                     /gene="rffT"
                     /locus_tag="OO439_001045"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6315462.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22614..23864)
                     /gene="wzxE"
                     /locus_tag="OO439_001046"
     CDS             complement(22614..23864)
                     /gene="wzxE"
                     /locus_tag="OO439_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6315463.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23866..24996)
                     /gene="rffA"
                     /locus_tag="OO439_001047"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23866..24996)
                     /gene="rffA"
                     /locus_tag="OO439_001047"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6315464.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25001..25675)
                     /gene="rffC"
                     /locus_tag="OO439_001048"
                     /gene_synonym="wecD"
     CDS             complement(25001..25675)
                     /gene="rffC"
                     /locus_tag="OO439_001048"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6315465.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25653..26534)
                     /gene="rfbA"
                     /locus_tag="OO439_001049"
     CDS             complement(25653..26534)
                     /gene="rfbA"
                     /locus_tag="OO439_001049"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6315466.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26553..27620)
                     /gene="rffG"
                     /locus_tag="OO439_001050"
     CDS             complement(26553..27620)
                     /gene="rffG"
                     /locus_tag="OO439_001050"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6315467.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27620..28882)
                     /gene="wecC"
                     /locus_tag="OO439_001051"
     CDS             complement(27620..28882)
                     /gene="wecC"
                     /locus_tag="OO439_001051"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6315468.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28879..30009)
                     /gene="wecB"
                     /locus_tag="OO439_001052"
     CDS             complement(28879..30009)
                     /gene="wecB"
                     /locus_tag="OO439_001052"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6315469.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30065..31111)
                     /gene="wzzE"
                     /locus_tag="OO439_001053"
     CDS             complement(30065..31111)
                     /gene="wzzE"
                     /locus_tag="OO439_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6315470.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31123..32226)
                     /gene="wecA"
                     /locus_tag="OO439_001054"
     CDS             complement(31123..32226)
                     /gene="wecA"
                     /locus_tag="OO439_001054"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6315471.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32466..33725)
                     /gene="rho"
                     /locus_tag="OO439_001055"
     CDS             complement(32466..33725)
                     /gene="rho"
                     /locus_tag="OO439_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6315472.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33810..33911)
                     /gene="rhoL"
                     /locus_tag="OO439_001056"
     CDS             complement(33810..33911)
                     /gene="rhoL"
                     /locus_tag="OO439_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6315473.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34052..34381)
                     /gene="trxA"
                     /locus_tag="OO439_001057"
     CDS             complement(34052..34381)
                     /gene="trxA"
                     /locus_tag="OO439_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6315474.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34512..35777
                     /gene="rhlB"
                     /locus_tag="OO439_001058"
     CDS             34512..35777
                     /gene="rhlB"
                     /locus_tag="OO439_001058"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6315475.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35911..37395
                     /gene="gppA"
                     /locus_tag="OO439_001059"
     CDS             35911..37395
                     /gene="gppA"
                     /locus_tag="OO439_001059"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6315476.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37442..39463)
                     /gene="rep"
                     /locus_tag="OO439_001060"
     CDS             complement(37442..39463)
                     /gene="rep"
                     /locus_tag="OO439_001060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6315477.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9864 a        10826 c        10044 g         9075 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcgac atcgttcgtc tggatgcctt caaaaccagt
      121 aaagagattc agtttgcggt gcagtcaggg ccaatgttga tggaaaacag tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttgatggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagagga aaggttaagc attgtacact cgaggaggcc agacaaactg gggctggcta
      481 cacagctgaa aaatcatcag ataaatgaag ggaaacaaaa atgaaaaaaa cgctcgcaac
      541 gttattttta ttaacgtgtc tgggatcatc atccgcttat gcggataacg ctttgatttt
      601 acaaactgat tttagcctga aagatggtgc cgtctcggcg atgaaagggg tcgcattcgg
      661 tgttgaccat aatcttaaaa tatttgattt aacacacgaa attccaccgt ataacatctg
      721 ggaaggcgct taccgcctgt atcagaccgc cagttactgg ccgcaaggtt cggtatttgt
      781 cagtgtggtc gatcctggtg ttggtacaga ccgtaaatcg gtagtgctaa aaaccaaaaa
      841 cggtcaatac ttcgtttcac cagataacgg tacgctcact ttggtggcgg aatcgctggg
      901 tattgagagc gtccggcaaa tcgatgaaaa aaccaaccgc ctgaaaggtt ccgaaaagtc
      961 atataccttc cacggtcgtg acgtgtatgc ctacaccggt gcacgcctgg cttccggagc
     1021 gatcacgttc gaacaggtgg ggccagagct tcctgcaaaa gttgtcgaac tttcttatca
     1081 gaaagccaaa gcaacaaaag gtgaagtgaa gggcaatatc ccgattctcg atattcagta
     1141 cggcaacgtc tggagcaata tcagcgacga gttgctgaat caggcaggaa tcaaactcaa
     1201 cgacacgttg tgtgtaacca tttccgaagg ttcccagcaa aaatacgcag ggaaaatgcc
     1261 gtatgtcgca agcttcggcg atgtgccgga aggccagccg atggtgtatc taaacagctt
     1321 attgaatgtt tccgtggcgc tcaatatgga taatttcgcg cagaaacatc aagttgcgtc
     1381 tggtgctgac tggaatattg atgtgaagaa gtgcgctaag taaggtgctg atgcaggatg
     1441 cggtgtggcg caccgcatcc tgcaacggag ttgtaaatta caccgtctcc agccgggcgt
     1501 aagccgccac cagccatttg attccctggc cctggaatgc cacctgcaaa cggctatgtt
     1561 caccgctgcc ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgcacgcgct
     1621 ggccgagttt gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctaa
     1681 ccgggcggct taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc
     1741 cgataaagcg cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt
     1801 aggtcagcgt cagtttctgc atcgcacggg ttacgccaac gtaggccaga cggcgttctt
     1861 cttccagacg tccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac
     1921 cgacgataaa cacctgcggg aactccagcc ctttcgccga gtgtagcgtc atcaactgca
     1981 ccgcatcctg ccaagtatcc gcctgccctt cacctgcttc cagtgccgca tgggagagga
     2041 acgcctgcag cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg
     2101 tcaccagttc ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt
     2161 acatggtacg caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat
     2221 cggcagtttc ttgcgctaag gcatcgatta attccataaa tcgctgcaag gcgctggcag
     2281 ctcgcccggc gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact
     2341 ggcgatcgcg cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg
     2401 gcgtattcac cacgcgctca aacgccgcgt cgtcgttacg gttggcaatc agacgcagat
     2461 acgagagcgc gtctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt
     2521 acggcatact ggcctgcaac agtgcttctt ccagtacacg cgactgggcg ttactgcggt
     2581 agagaatggc gcactcggca agcgccccgc cgttgtcctg ccaagttttg atgcggttaa
     2641 ccacaaaacg cgcttcgtcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt
     2701 ccgcgccatc ggtccacagt tttttaccca gacgcccgtt gttgttttca atcagggcgt
     2761 tcgcggcgct cagaatattg ctggtagagc ggtagttctg ctccagacgg atagtttcag
     2821 caccggggaa atcattgagg aaacgctgaa tattctctac ctgcgccccg cgccagccgt
     2881 agattgactg gtcgtcatcg ccaacgatca tcactttgcc ggtatcgccc gccagcaggc
     2941 ggatccacgc gtactgaatg ttgttggtat cctggaattc gtccaccagg atgttggtaa
     3001 aacgttcgcg gtagtgttgc aggatatgcg gcttgttaag ccacaactcg tgagcgcgca
     3061 gcagcagctc ggcgaagtcc accaggcccg cacgatcgca cgcttcctga tacgcctgat
     3121 acaccttctg ccaggtctgc tccaccggat taccgtagct ttgaatatga tgcggacgca
     3181 ggccttcatc tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct
     3241 cgtcgaggtt catggctttg atcagacgct taagcaggcg tagctggtct tcactgtcga
     3301 ggatctggaa atcctgcggt agattggcgt ccatatggtg cgcacgcagc aaacggtgcg
     3361 ccagcccgtg gaaggtgccg acccacatgc cgccctggct ggtgcccatc agttgcccga
     3421 tacgatgacg catctccgcc gccgctttgt tggtaaacgt cacagccata atcgagtatg
     3481 gcgagcagtt ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac
     3541 cactgcccgc gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct
     3601 gtttgtcatt aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat
     3661 aggttgggcg gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttac
     3721 tgattatatc agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg
     3781 gctgtcccag gtttgcatca gatcgccatt ttccggtctg atccaacaag cctgcattcc
     3841 gctgcgaatt gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc
     3901 gatcggcacg ttgagttttt ccgcagccaa aaagtacata tcgctgaacg gttttgagcg
     3961 cccatgcggg ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg
     4021 ctgggcgtta ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt
     4081 gtcgtgagtt tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt taatcattgc
     4141 tgcgtgtgcg cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc
     4201 aatcgaacga aaacgccaac gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac
     4261 cgcctggcgc aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt
     4321 ttgcacaaag gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag
     4381 ggtatcatcc aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat
     4441 tatttccccc gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa
     4501 tcaagatgag tatagatttg cgtggtggag aggttggcat gacccagcag ctcctgcaca
     4561 ccacgaagat cgccgctcga ctccagcata tgcgtggcga acgagtgacg taatttatgc
     4621 ggatgaacgt gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca
     4681 ttacgcgcgg agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca
     4741 aacaggtcgc gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc
     4801 aggcggcgct ctttgctgcc tttccccatt acccacactt caccagactc caggtcgagg
     4861 tgtttgatat ccagccccac cagctcggaa agacgcagac ccgcgccgta catcacttcc
     4921 agcattgcac ggtcgcgtac agcgagggga tcgttgatat caatgtccag cagccgattc
     4981 atatcgtcga cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct
     5041 ttcgccgggt tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc
     5101 gcagaaagcc gtaacgcgag gcttgctgct cccagccctt tacggcgact gcgtacagca
     5161 aaattgcgca ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca
     5221 aaattgatga tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg
     5281 cgctccacgc tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc
     5341 gttcaatcca acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg
     5401 ttccctgccc ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat
     5461 cagcatcgct tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca
     5521 ccaccagcag ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct
     5581 gaatacgcag cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg
     5641 cacctaagcg ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc
     5701 gcgcccagcg gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc
     5761 gctgcaggta gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca
     5821 gcgccatgtt ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga
     5881 ccaacgaaac ggtgccgcgt accggatgcg gcacacgtat cgcttctact gcgcgcgcat
     5941 tacggataaa aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct
     6001 ccgtgagtgt ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccgtcgta
     6061 gacatgtacc gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc
     6121 aagacgaccg ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc
     6181 tactgcaacc gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc
     6241 ataaacgcgt aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg
     6301 ctccggaaaa cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc
     6361 gacatcatcg acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat
     6421 tgtctgctcg gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac
     6481 ggcggaaggt tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac
     6541 ggtcagaacc atccgcccgt tggcggtgct gacgcggata tcacgcttat tggtcagtcc
     6601 tttcagacgc acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact
     6661 gccatcagca ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac
     6721 caccagcagt tggtcaaacc ctacccccag gtgccgatca gccaggcgac gaatcagctc
     6781 cggtgaaaaa aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc
     6841 atgcattttc gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta
     6901 gtaattcacc tgggatggac catcgccagt ggcgcgatcg tttttatccg gcaccgtgga
     6961 ttgcgtttgc gtctctaccg gtttggtcgg cggcggtgcg tttttatctg caggcgggaa
     7021 atagagcgga cctttcagac cgcagcccgt caggctgaag agagtaagta atacagtgag
     7081 tgccttaaac acgtttttca ttatggattg cctgtaaagt tcatcgcttt ctgctttctg
     7141 ctttctgctt tctgctttct gctttctgct ttctgctttc tgctttctgc tttctgcttt
     7201 ctgctttctg ctttctatca tcgcaggaga agacgtaaaa gcaagcgttt agcgactctt
     7261 tatgaagtct gaaaagtgag gaaatgctgc gagtataacg cttactcgtg gggatttatg
     7321 ttttgctggt agtttcgtga tcggcacctc gcgccaggat ggctctatgc actatcgcaa
     7381 tgtggtttgt ttgttgtctt ttttagttcc gtcagcgtga ggatgctcgc tcggtaaacc
     7441 tgatcataat gtttaccaga aacagggaaa ggcgtggtgt aactgcgccc taatgaagag
     7501 acaaatcttt cgctacacag agacatcatt attccggtga cggatgaatc gtgccggaac
     7561 gcagccgcag aggtgcaaac tttgaagccc tgaccccatg ccgctatact gccgccatca
     7621 cttaaaaaca ggatacaaca atgaacgaca gtgaatttca tcgcctggct gatcaactct
     7681 ggctgaccat tgaagaacgc ctggacgact gggatggcga cagcgatatc gactgcgaaa
     7741 tcaacggcgg cgtgctgacc attacctttg aaaacggcag caaaatcatt atcaaccgcc
     7801 aggaaccgct gcaccaggta tggctggcaa ccaaacaagg cggctaccat tttgatctga
     7861 aaggcgatga gtggatttgc gatcgcagcg gcgaaacctt ctgggatttg ctggaacagg
     7921 cggcgacgca gcaggcgggt gaaacagtca gtttccgcta agattgcatg ccggataagc
     7981 ctcgctttcc ggcacgttca tcacgaaaaa tattgctgta atagcggcgt atcgtgatcc
     8041 tgattggcag gcggcatgtt accgatagat tttgtgcgga acggaatcac ctgttcacga
     8101 ccatcaacct tcacaatctg atagaactgc ggcaggttga agttgatgaa gcttgagccg
     8161 taggtgaagc ggtcgtgcga tgacgagtag aagcgactga cgtcacgcac cagttcctct
     8221 ttgctgcctt cgcagtggtg atacacctca acccggttgc tttcgtcaag aatgtagata
     8281 ttaaagccat tctcgtcttg cgtttcttcg aagaagaact ggatgatccc ttcactagca
     8341 aagccgtcca ccaccgccgg taatttgacg tgattggttt caacctgcac tgacaggccg
     8401 tgcagtttgt tatgcgaaat agcgccataa aactcgatgg cgttctccag cttctgtacc
     8461 gatacattca ggcgttcgaa gaacaagccc caggtttgac cagaaacgcg cagcgccttg
     8521 aaacgcccgg tttcctggcg ggtgctggaa aggcgcagtt caatacactc agaaaccagt
     8581 tgctgcacgc gagtacggat taacccgcgc agatgctggc tataacagaa gacttccacg
     8641 ctatctggcg gtgcggcgtc ctgatgcatt ttgccgagaa tagttttcag ggcttcgatc
     8701 atcgattgct cgccgttgaa gtgcagcgta cgcacttcgt tccacgagtt gcggtacagc
     8761 aggtcaacgc tacctaccag gcaattttga ttctcgccaa agctgaagac atccagctta
     8821 cggaaatcaa aatgcaccac ctgattgcgg aacgccgctg tcgggtcata ttccaggtta
     8881 acgataatcg ccagatggcg gatctcacac gggctgtaga gcgccttcgg tgtcggtgca
     8941 ggtaagcgca gcgggaaatg gtgcgacacg tcggcgacca tctcctgcaa cttaggcaaa
     9001 tcgacaatgc cgttaccttt aatatacaaa cgggtgcgcg aggtcagcag accgttaaac
     9061 catgcccacg ccaccagttt attcaggtaa cggttatatt ccagcggctg atggctgatg
     9121 atcgactcaa tatttggcgc gcggttatac agataccaac ctgaacggtt agcccggccc
     9181 ggcggcacat aaataaaggt cagattcggt tccgagagat cgggtgaaat ctgcgggttt
     9241 accagcgtca ctttacctgg taatgcttca aacgcggcat acagcttacg cgtcagcacg
     9301 ccgatatcct gcggactggc ggagacgcta aggttattgc gacgcgcaaa gcggatcaga
     9361 ttacggtagc tctgcatcat cgcgtcgagc aactcgttgt gcgcctcacg cacctgatca
     9421 atcttccagt tagcacggtt atcgagcatt gccagacgag cttcgtccca accccactcg
     9481 ctcactaact ggctcaacac tgcgcgacgc cagcctacac aggcgcgttc acggctgagt
     9541 ttttcgcaca cttttaaata gaagcagcga cgtactaaat ccagacgggt gaaatcttca
     9601 atcgccgtca ggtattcagt aacacgctcc agcatcatgc agtatggatc gaggccaaac
     9661 gatacaatct cgccgtcgtg caaacgctgt ttgatatctt tcgccagcag acgtgggttc
     9721 gggtattccc aggaataggc ttccagcagc agtgttttca gcaccgcttt gtatggggaa
     9781 tcgatactct tgtagagctg ccaaaggctg gcaccaaagt actcttcagc agaaagcgag
     9841 cttaagccac cgagatccag ccattcattt ggcgtcagca cgccctgcgc gtaaagcgtc
     9901 atcacatagt cgtcgtaatg ctcttcttcg tcgcacggca ccatattcca cagaatacgc
     9961 ttaccggcga gacgcacggc ggtacgataa aattcgtcaa gcagcagtat atgctgggtg
    10021 gagccacaat cttcgccccc caggctgccg ctttcattat gacgaaagcg gttttcatca
    10081 atcaggaaga agctgacttc cacacccagc gaggcggccc agctttccag caggctgcat
    10141 ttacgttgta gtaactggcg ctcttcgcta tcgagccagg attgatgaca gacccagata
    10201 tccaggtcag aggaacaact ttgccctacg gacgaggtgc tgcccatggt gtatacacca
    10261 gtaattggaa gctcaccttt cggtggatcc tgtactgaca ttccacgata cagttcaagc
    10321 tcgttcaggt agtggcgttg agtttcatca ggcgtgtaaa ggcaaatgcc tttgggaacg
    10381 ttaccatcaa ggtaacccgg cattagcgga tggtgatagt gcaacaatgt cggcagtaga
    10441 ctgtagacct gttggaatgc aggccccata gcagcaagcg cgcgatccac acgcaattga
    10501 tttatggcat ccagtctctg tttcagagtc tcaatataga ggtacaagac gtatcgcctg
    10561 atttgctacc cgtcatgact gtgattccgc caacatcaac ggtaacacgc ggcattcggg
    10621 atatttcgta tgtcaaaggt aaccgttacc acttttcgcg cctggttttt ttagtttcac
    10681 gacgaaaaaa tggtctaaaa cgtgatcaat ttaacacctt gctgattgac cgtaaagaaa
    10741 gatgcgctac atacaagtgt agcaccgttt attctctgta aattccttat tacgacagcg
    10801 tgaaacgtct gtcaggatcc actgccagac ctcattttac ggtttgcgca ggcgtctacg
    10861 tttcaccaca acactgacat cactctggca aggatgttag gatggaccac ggatgataat
    10921 gacggtaaca agcatgttag acaatgtttt aagaattgcc acacgccaaa gcccacttgc
    10981 actctggcag gcacactatg tcaaagacaa gttgatggcg agccatccgg gcctggtcgt
    11041 tgaactggta ccgatggtga cgcgcggcga tgtgattctt gatacgccgc tggcgaaagt
    11101 aggcggaaaa ggcttatttg taaaagagct ggaagtcgcg ctcctcgaaa atcgcgccga
    11161 tatcgccgta cattcaatga aagatgtgcc ggttgaattc ccgcaaggtc tgggactggt
    11221 cactatttgt gagcgtgaag atcctcgcga tgcctttgtg tccaataact atgacagtct
    11281 ggatgcgtta ccagcaggca gtatcgtcgg gacgtccagt ttacgtcgcc agtgccaact
    11341 ggctgaacgc cgcccggatc tgattatccg ctccctgcgc ggcaacgtcg gcactcgcct
    11401 gagcaaacta gataacggcg aatacgatgc catcattctt gccgtagccg gactaaaacg
    11461 tttaggtctg gagtcccgca ttcgcgccgc gttaccaccc gagatttctc ttccggcggt
    11521 aggacaaggt gcagtcggta ttgaatgccg ccttgatgat acgcgcactc gcgagctgct
    11581 tgccgcgctg aatcaccacg aaactgcact gcgcgttacc gcagaacgcg ccatgaatac
    11641 ccgtctcgaa ggcggatgtc aggtgccaat tggtagctac gccgagctta ttgatggcga
    11701 aatctggctg cgtgcgctgg tcggcgcgcc ggacggttcg cagattattc gcggtgaacg
    11761 ccgcggtgcg ccgcaaaatg ccaaacaaat ggggatttcg ctggcagaag agctactgaa
    11821 taacggcgcg cgcgagatcc tcgctgaagt ctataacgga gacgccccgg catgagtatc
    11881 cttgtcaccc gcccgtctcc cgctggagaa gagttagtga gccgtctgcg cacactgggg
    11941 caggtggcct ggcattttcc gctgattgag ttttctccgg gtcaacaatt accgcaactt
    12001 gctgatcaac tggcagcgct gggggagagc gatctgttgt ttgccctctc gcaacacgcg
    12061 gttgcttttg cccaatcaca gctacatcag caagatcgta aatggccccg actacctaat
    12121 tatttcgcca ttggacgcac caccgcactg gcactacata ccgtaagcgg acagaagatt
    12181 ctctacccgc aggatcggga aatcagcgaa gtcttgctac aattacctga attacaaaat
    12241 attgcgggca aacgtgcgct gatattacgt ggcaatggcg gtcgtgagct aattggggat
    12301 accctgacgg cgcgcggtgc tgaggtcact ttttgtgaat gttatcaacg atgcgcaatc
    12361 cattacgatg gtgcagaaga agcgatgcgc tggcaatccc gcgaggtgac gacggtcgtt
    12421 gttaccagcg gtgaaatgtt gcagcaactc tggtcgctga tcccacaatg gtatcgtgag
    12481 cactggttac tacactgtcg actattggtc gtcagtgagc gtttggcgaa actcgcccgg
    12541 gaactgggct ggcaagacat taaggtcgcc gataacgctg ccaacgatgc gcttttacgg
    12601 gcattacaat aactctcata acaggaagcc ataatgacgg aacaagaaaa aacctccgcc
    12661 gtggttgaag agactaggga ggccgtggac accacgtcac aacctgtcgc aacagaaaaa
    12721 aagagtaaga acaataccgc attgattctc agcgcggcgg ctatcgctat tgctctggcg
    12781 gcgggcgtcg gtttgtatgg ctggggtaaa caacaggccg tcaatcagac cgccaccagc
    12841 gatgccctgg ctaaccaact gacggcattg caaaaagccc aggagagcca aaaagccgaa
    12901 ctggaaggca ttattaagca acaggctacg cagcttgaac aggcgaatcg tcagcaagaa
    12961 acgctggcaa aacagctgga tgaagtccag caaaaggtcg ccaccatttc cggcagcgat
    13021 gctaaaacct ggctgctggc ccaggccgat tttctggtga aactcgccgg acggaagctg
    13081 tggagcgatc aggacgtcac gaccgctgca gcgttgctga aaagtgcaga cgccagcctg
    13141 gcggatatga atgacccgag tctgattacc gttcgtcggg caattaccga tgatatcgcc
    13201 agcctttctg cagtatcgca ggtggattat gacggcatca tccttaagct taatcagctt
    13261 tcaaatcagg tagataacct gcgtctggcc gataatgaca gcgatggttc gccgatggat
    13321 tcagacggtg aagagctttc cagttccatc agcgaatggc gtatcaatct gcaaaaaagc
    13381 tggcagaact ttatggacaa cttcattacg attcgccgtc gtgatgacac cgccgtaccg
    13441 ctgttagcgc caaatcagga tatctatctg cgcgaaaata ttcgctctcg cctgctggtc
    13501 gcagcacaag ctgtaccgcg tcatcaggaa gagacttatc gccaggcgct ggagaacgtc
    13561 tccacctggg tacgtgctta ctacgatact gatgatgcca ccaccaaagc gttcctcgac
    13621 gaggtggacc agttaagcca gcaaaatatc tcgatggatc ttccggaaac cctgcaaagc
    13681 caggcgatgc tggaaaaact gatgcagacc cgcgtgcgta acctgctggc acaaccggca
    13741 gcggggacaa cggaagctaa acctgcacct gcacctgcac ctgcacctgc acctgcacct
    13801 gcaccgcaag gtgatactcc ggcagccgcg ccgcaaggag aataaccatg ctaaaagtgt
    13861 tattactctt tgttttgctg attgcgggga tagtggttgg cccgatgatt gccggtcatc
    13921 agggttacgt gctgatccag actgacaact acaatatcga aaccagcgtc acgggcctgg
    13981 cgattatttt gattctggcg atggtagtgc tgtttgccat tgagtggcta ctgcggcgga
    14041 tcttccgcac aggcgcacac acccgtgggt ggtttgtcgg acgtaagcgt cgccgtgccc
    14101 gtaagcagac cgaacaggcg ctgctgaaac tggcggaagg cgattatcag caagttgaaa
    14161 agctgatggc gaaaaatgcc gatcacgcgg aacaaccggt ggtgaactat ctactggctg
    14221 ccgaagccgc gcaacaacgt ggtgatgaag cacgcgccaa ccaacatctg gaacgcgcag
    14281 cagagctggc cggcaatgac accattccgg tagaaatcac ccgtgtacgt ctgcaactgg
    14341 cccgtaatga aaaccatgct gcacgccacg gcgtggataa gctgctggaa gttacgccac
    14401 gccatccgga agtattacgt ctggcggaac aggcgtatat ccgcacaggt gcatggagtt
    14461 ctctgctgga tatcatccca tcaatggcga aagcccatgt tggtgatgaa gaacatcgtg
    14521 caatgctgga acaacaggca tggattggcc tgatggatca ggcgcgtgcc gataacggta
    14581 gtgaaggttt gcgtaactgg tggaaaaacc aaagccggaa aacgcgtcat caggtagcgt
    14641 tgcaggtggc aatggcggaa catcttattg aatgtgacga tcatgatact gcccagcaaa
    14701 ttatcatcga tggcctgaaa cgccagtacg acgatcgcct actgctgccg attcctcgac
    14761 tgaaaaccaa caatccggaa cagctggaaa aagtgctgcg ccagcagatc aaaaacgtcg
    14821 gcgatcgccc gctgttgtgg agcacactgg gccagtcact gatgaagcac ggagaatggc
    14881 aggaagcatc gctcgccttc cgcgcagcgc tgaaacaacg tccggacgcc tacgattacg
    14941 catggcttgc cgacgcgctg gacagactgc acaagccgga agaagctgca gcgatgcgtc
    15001 gcgacggttt gatgttaacg ttgcagaaca acccaccaca gtagttcctt ctcacccgga
    15061 ggcaagcacc tccgggacct ttctgatgca taaaaaaacg cctgctctta ttacggagca
    15121 ggcgttaaaa caggtctgta tgacaacagt gggtgcttca ctcaacgttg tgtccatggt
    15181 gtctgatgag gcataagcga catctgtcag tggacgataa gcaccgtaaa cggctctgcg
    15241 tcattccgga gtttatgagg cactaaggcg aacataagag atggaatgag catctacccg
    15301 tttattatgc cacagagaat cgggaaataa catcccttaa cacttgttat gagataattc
    15361 tgtaatcctc tttgcttcct gagtgaatat ttaacctgag cttgatccta cacatactta
    15421 ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa
    15481 aaagcattaa taatcaacgc aatataataa acccgcctta catatcaact gcgccagagg
    15541 taggattgaa aacgctctcc tgattttcca attcattttc tggataaata aataatttat
    15601 ttttgtcact attatttatg taatcatcct gtcagggaga gggatctcaa ttatcaatgc
    15661 ttaattacgt catcattttg atgacatgaa accggagcat tttgcatgga attttcgttt
    15721 tcacccaaac ttcttgttgt tgctgtcgcc gccgctcttc ctctcatggc cagcgcagca
    15781 gataccccgt caactgccac cgcacgcaaa ggctttgccg gatacgatca cccaaaccag
    15841 tatccacaga acgctgcaat ttattgaatt tgcacgatca tgtaggccgg ataaggcgtt
    15901 tacgccgcat ccggcaatca accgcaggcg gccacaaaat ggccgctttt ttacttattc
    15961 accaccagca aatgtgcctg cataatgtca ctggccgggc gaccgtgcgc cagcaaatca
    16021 gccattgctt taagatatgg cggtagatgg cggaaataac gctgataccc ggcacacaaa
    16081 taattcagcc ccggtttgcc gctggcatcg agcatgaagc ggtgtttcgg gcagcctccc
    16141 cagcacgctt ttaacacgtt acaactgcga cactgcgccg gtaattgctt aaatttatct
    16201 tcaccaaaca cctgctgttg cggggaatcg atcatttctg caattgtttg ctgatgcata
    16261 ttccccagcc gatattgcgg ataaacatag tgatcgcagg cgtaaacgtc gccgttgtgc
    16321 tcaacaatca ccgagcgccc acaggttggc tgatgatggc aaaccgcacc cggcgcaccg
    16381 acaaaattgg caaacgccca ttcgatattc atcacgaaaa tcttgccgac gtcgcgtttg
    16441 atccagtggt cgaatatcgc caccagaaat tcaccaaatt catcggggcg caccgaccat
    16501 tccgtcagtt ccccctgaat atcaccaggc gcatgcaatt tcagtccatc gcgggcagct
    16561 gtttcatcag ccagacgctc gaccaccgga ataaactgga tgaattcgac tcccgcatcg
    16621 cacaaaaaat cataaacctg caacggttgc tgcgcgctgg tgcggttgac gcagaccagc
    16681 acgttatagt cgacatgatg tttttgcagc agcgtcaggg cacgcatcac cagcttatgc
    16741 gttggtctgc cacctttggt cacgcgatat tgattgtgga tctcagccgg accgtccagc
    16801 gataacccaa caagaaaatg attttctgcc agaaatgcac accatttatc atcgagcagc
    16861 acgccgttag tctggaagct gttacttatc ttcctgccag caccgtattt cgcctgtaac
    16921 tttacggcac ggcggtaaaa atccagcccg agtaacgttg gttcgccgcc ctgccaggta
    16981 aaagccactt cgttttgcgg ttcgctggca gcgatatagt gacggacata cgcttccagc
    17041 gtgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa gggattgttt ttcgagataa
    17101 aaacagtagt cacagttcag attacaatcg gaaccactcg gtttcgccat cacatgaaaa
    17161 gcacgcgttg gaacctgttg cagcatggtc actccttgcc tgaagtcatc cttcaatacg
    17221 ttaagggcga aatcataccg tggggcaatg agttattacg ggcgtttatt gcgaagggag
    17281 ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc ggggttcaaa attggtcggc
    17341 gagagaggat tcgaacctcc gacccactgg tcccaaacca gttgcgctac caagctgcgc
    17401 tactcgccga aatactgctt tttgaatttt tagttcaatt ctttaaagtc gtggtgcgag
    17461 gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac
    17521 caattccgcc accttcgcat tgtcacaact tctaataatg gggtggctaa tgggattcga
    17581 acccacgaca actggaatca caatccaggg ctctaccaac tgagctatag ccaccactac
    17641 aaatcttgtt acgcggtatt actaccaccg cagctcaagc gccgggacta aatggcgcgc
    17701 ccgacaggat tcgaacctga gacctctgcc tccggagggc agcgctctat ccagctgagc
    17761 tacgggcgct tagcgccgtt gcggggggat aatacggact tcccacccct ctgtctagtc
    17821 cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct tattcctcca
    17881 gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc gtcaccgcca
    17941 gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag tacatgccga
    18001 tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac aggattgagc
    18061 ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc tggcttatca
    18121 atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg tagacaaaca
    18181 cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga atagcaatag
    18241 atactgccac acccgcaact ggtacgccgt gacgggaaac tttcgccatc gccgccggta
    18301 actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg ccgctgttac
    18361 agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct gcggtaatac
    18421 cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccaatttca ttccacggga
    18481 agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc cacagcactt
    18541 tgcctacagc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca gtaatgccaa
    18601 tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg aaccctttcc
    18661 agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc gactgcccgc
    18721 cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg ataatcgtgg
    18781 tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc gccgccagat
    18841 tcgccaacgc caccagcgcc accgcgatca atgcaggtat ccactgcgcc atctccggga
    18901 accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc gccatccaca
    18961 taaaccagta agaccaggcg gtgagataac caaaaaacgg gctcatataa cgatgcgcat
    19021 aaacggcgaa cgaaccggta acaggttcaa ggaacaacat ttcgcccatt gaacgcatga
    19081 tgaaaaagac gaacagcccg gcgatgatat aggccaacaa tacggatggc ccggcccatt
    19141 tcagggtact ggcggccccc ataaacaggc cgacgccaat agtgcccccc agggcgatga
    19201 gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct gccataaatc
    19261 ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt acatcgagtg
    19321 ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa tttgaatgct
    19381 ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt acaaagagag
    19441 gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg aagctgacgc
    19501 ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag cgtttgccag
    19561 attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc aacgcccata
    19621 tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg ctttggcgat
    19681 cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa cagcgcctga
    19741 cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt ccattggttg
    19801 cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag aaataccggc
    19861 gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc ggcaacgcgg
    19921 gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat gccatccgca
    19981 tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc aatggtcagc
    20041 attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc agcaaacaga
    20101 taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg cagcgtatag
    20161 gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg agcgatgatt
    20221 ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacagcagt tttgcgatca
    20281 tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac gaatccagcc
    20341 cttcacgcgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg tgcaatatcg
    20401 cagccttata gcgattaggc tcgcggttgc ccagctcata cagccagtcg aaccatttga
    20461 tgatcagacc aaccacgatc gccccgagcg ggatgaacaa cgcgccgccc atcaccacca
    20521 gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc acttcccagg
    20581 taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc caggaaggga
    20641 taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg ttgtcgtagt
    20701 tctgcaacag caacgccaga ttctcccacg gggagaaggt gtcgcgagtg agatagagaa
    20761 acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc gccagccaga
    20821 acatgccaac aatccccagc acgcccgccg ccgccagcat ccacaacgaa atccagccgc
    20881 gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg cgagtgccgc
    20941 cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa aacagccacg
    21001 ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga
    21061 agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag gagttaagcc
    21121 ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc gcgataccca
    21181 tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg
    21241 gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa accgcgtaga
    21301 agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa
    21361 agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc aaaaacagca
    21421 atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg
    21481 caataaacag cgtgcagagc agccagacca caaacaggcc actgaattgc agcagactca
    21541 tgcgacctcc ccggcggcaa tagccaacgc ccgctgccag ccttgtagat agttagggct
    21601 aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt cacgcacaat
    21661 atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg tcatatcctg
    21721 ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca gtaagcaaag
    21781 cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact gacgaagtag
    21841 cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt cttcgctgaa
    21901 taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag gcggatatcc
    21961 catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc gcaaggcagc
    22021 aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt tcccttcacg
    22081 ttgccgatca ttcgccatcg tattgagaga agggtccatc cgcgtcggga agtacagcag
    22141 ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcaccgc gggtggcaaa
    22201 tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct tatatctcaa
    22261 gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact ggctgggctt
    22321 aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt ggaaaaaaaa
    22381 tcgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg attttttccc
    22441 agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt ccttgccaac
    22501 aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga aaaaccgcaa
    22561 aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta cagtcatgcc
    22621 cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat ataagttgcc
    22681 atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc
    22741 atcaataaag tgaactggct gacttccgcc agaatataaa accgcagtga cgctttggcg
    22801 atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac taactgccag
    22861 gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcaaccagat agcaaaatca
    22921 cgcagcaacc agacggtaaa actcgccgcc gccactgccg gtaagacgaa tttcagcgat
    22981 ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa
    23041 tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact gctcacccct
    23101 tgccagatcc ccacttcatc ccagctatac tgcgccgcca gcagtttacg catcatgatg
    23161 taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt gctcaactgc
    23221 cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac accacgtttg
    23281 atcaacatga tggcggcagg aatcaccacc agcgcgggaa tcagcgccag acccagcaac
    23341 gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc aatcaagctg
    23401 ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat cagcgccagt
    23461 aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac cagcccctga
    23521 tagtcggtat taccaaacag tccctggctg attggcgcag ctgccagcac aaaaaccagc
    23581 gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac gcggcgcagc
    23641 tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa gatgccagct
    23701 ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag cccaagcccc
    23761 gccggaccaa atgacacagc cagcaactta ccgaccagta acccggcacc aatcttgacc
    23821 agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg agaagtagtt
    23881 caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga acagcggcag
    23941 gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga actcaccaaa
    24001 gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc
    24061 ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt taatgtagaa
    24121 catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac gcccggcttt
    24181 cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt gctggttgat
    24241 acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg acatcaaata
    24301 gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga actggctgcg
    24361 gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat cgttaatcag
    24421 cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt tcatggaagc taaagcagcc
    24481 aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc
    24541 atcttctacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt ccatttcgca
    24601 ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc
    24661 aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc
    24721 gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca tgatcacttc
    24781 atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg tgcaggacgg
    24841 cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc agcgacgggt
    24901 aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat agtcgagttc
    24961 ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat aaccagtacg
    25021 cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg gtgttgccca
    25081 tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt agcgccgttt
    25141 gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat
    25201 tgagttcccg taaagagaca taaccgcgaa taccgccgga cgccgcacgt aaaatcagac
    25261 attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa aagcgtccgc
    25321 tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta
    25381 actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta tcactgacat
    25441 tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc agctgttgca
    25501 gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt gaccacggcg
    25561 ctaacacgtc tggcgtcagg agctgtgctt cagacgtaat gcgcacgatg gcgctgttaa
    25621 caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg ggcacgaagt
    25681 aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc acgcttcaca
    25741 ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc aatcttaaag
    25801 ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct gtcgtgagtg
    25861 ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg
    25921 aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg cttcacctgc
    25981 tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc ccagtttgac
    26041 ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc aaactccacc
    26101 acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc ttccgtgcgc
    26161 gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt atcgcccagc
    26221 accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc ctgcgccaga
    26281 ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact accatcgccc
    26341 agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat ttcgcggata
    26401 tcggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta aatcggcaac
    26461 agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg
    26521 ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa acgctcgccc
    26581 tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata ccactgcacc
    26641 gttttacgca ttccactttc aaaggtttcc tgtggcagcc agccaagttc acgggcaatt
    26701 ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac aaaggtgatc
    26761 aagtcacgat agtgcaccac gccgtgcggc ttgttcggag ccagttcttc cagcagctcg
    26821 caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat attataggtt
    26881 tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac
    26941 agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc cgccagcgcg
    27001 ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta gttattcgag
    27061 cagttggtga taagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac cagatggtcg
    27121 ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt ttcggtgaag
    27181 aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg atgaaaacgg
    27241 aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc ttcaagcaat
    27301 gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat agaacggtca
    27361 acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt gaatacgcgt
    27421 gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc gctttgcgcg
    27481 accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac taccaccgcg
    27541 tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc ggcaccacct
    27601 gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca tactgctgat
    27661 ggacattgtc gccattgata actttaaact gactatgatc gaccagcatc accagcacgt
    27721 ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc agttttttcg
    27781 gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac tgggcgatca
    27841 gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt ttaaacgcca
    27901 gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagccgcc aggcaatcag
    27961 ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc acttcgcgcg
    28021 cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac ggatcaacag
    28081 caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga gggtgacgat
    28141 tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc agcgacaatt
    28201 cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta cacatttccg
    28261 ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac agttcgctgg
    28321 cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta atcagctcga
    28381 ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg tccgcctgct
    28441 cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat tctgccatct
    28501 tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct tttttcagca
    28561 ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca tgatcgcctt
    28621 taaacggcgt gggtacagca atcagccagg catccgcttc aactggcgtc gtgctcgctc
    28681 gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggc tcgacgatat
    28741 ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct
    28801 gtttttgccg tgaggcaaac gctgctgctg taggcagccc gatgtaaccc agtccgataa
    28861 cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt ccagaatgcg
    28921 agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagttt gatattcgtt
    28981 ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg tgcctaccag
    29041 acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac
    29101 cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca aaatcagcca
    29161 ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca gaatgacatt
    29221 tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga gatgcaccgg
    29281 atagacaatc tggatgtcct ggtgcgtggt ggcaatgtct gccaacgcgt ggcagatttc
    29341 ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca tctttttatc
    29401 ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtatcgc tgctcatcac
    29461 ctggtcacgc acccataaca gtgcatcaat aactgtatta ccagtaatga agattcggct
    29521 atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag agaagtgata
    29581 catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg aataaagatc
    29641 gcccgtgcgc agaccagcct caacgtgacc aacaggaata cgctgataaa acgccgccag
    29701 gctggttgcc agcgtcgtcg ttgtgtcgcc gtgaaccagc acgacgtctg gtttgaactc
    29761 ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca ggccctgtcc
    29821 tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca gcacctgatc
    29881 gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag gatcttttgc
    29941 caacgcatgc accaacggcg ccatcttgat ggcttccggg cgcgtaccaa atacagtcag
    30001 tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctca ccgcagcagt
    30061 gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc cccccgacaa
    30121 tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc ggttcttccg
    30181 gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca acattcaggg
    30241 tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg acggcctgta
    30301 aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct gaatcaggta
    30361 attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca gcaattttca
    30421 gcgcctgttc aatactgttc atccggcggt cgtagatggc tttcgccacc tcttcctgac
    30481 gcttcacctg tgctttcatc tggatggtac gcgccgccca tgcgcctttc agctcatcat
    30541 tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac aggttattag
    30601 cgtcaggcgc ggtttcagca ataagcttca cgctgtcatt gaccgcgcgg gtaaagtctc
    30661 cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg gctttgctgt
    30721 tgcccaccat ccgctgtttg taatagtcgg tttgcagcca gaactctctg cgggtatccc
    30781 acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat ggttggtcgg
    30841 cagaagccat gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc gagtaatatc
    30901 cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgagctccac tcctgacgag
    30961 caaaaaaagt atacgccagc gcgattaacg caaatgccag ccccatgcca ataatccata
    31021 gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt tcagcgtctt
    31081 cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg ttaaattggg
    31141 gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt
    31201 ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga gcaccagcat
    31261 gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag cgagcagtgc
    31321 cgcagcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac gcatgatcaa
    31381 atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca ggcgacggta
    31441 cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg taaccgggct
    31501 gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg taaaaccaat
    31561 cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga taccaaggtt
    31621 aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg cgaggctggt
    31681 ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg acaacccgcc
    31741 cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc agacggcaaa
    31801 tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa agatataacc
    31861 caggctactg agataaagtt tgccgaacac catcataaca atgccaacag cggcctgtat
    31921 ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag
    31981 cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga caattccgaa
    32041 cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca atccctggtg
    32101 acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct tacgggcaaa
    32161 aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca ctgtcagtaa
    32221 attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg aagaccttta
    32281 ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa ccatttgata
    32341 tgcaaattgc ataacacgcc agcaaattcc aggaattttc ctaacatcgc cagcgcggca
    32401 ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt tggcataaga
    32461 caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt catggccagt
    32521 ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg
    32581 atccacattt tctgcagttc ttcctgagta gtgagcagtt cttctttacg ggtaccggaa
    32641 cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt
    32701 tccatgttgc ctgtaccttt aaactcttcg tagataactt cgtccatttt agaaccggta
    32761 tcgataagcg ccgtcgcgat aatagtcagg ctgccgccct cttccacgtt acgcgccgca
    32821 ccaaagaagc gtttcggacg atgcagggcg ttggcatcca caccaccggt caacacttta
    32881 cctgacgccg gaacaacggt gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga
    32941 atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac catttccgca
    33001 acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc accttttacc
    33061 agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac catcagcaca
    33121 caatccgggt ggttgtaagc aatgctctgg gcaatgttct gcagcagcat ggttttaccg
    33181 gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga tgccagatcc
    33241 agtacgcgag cagttaaatc ttcagtagaa ccgttaccac gttccatacg cagacgagaa
    33301 tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg
    33361 tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc tttcggcggg
    33421 cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat ttggctaggg
    33481 gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg gaggaaacca
    33541 aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc actctttgcg
    33601 tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tgcgagccag gttttccagc
    33661 cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa ttcggtaaga
    33721 ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt tgtgaatggt
    33781 atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg ctgcctggtc
    33841 acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc agaaatctgt
    33901 tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca aacttaataa
    33961 ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc gtccagtttt
    34021 tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg aggaactctt
    34081 tcaactgacc tttagacagt gcgcccactt tggttgccgc cacttcaccg tttttgaaca
    34141 gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg ttttgatcga
    34201 tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca tccagaatcg
    34261 gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg atcgccccgt
    34321 ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca
    34381 tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt aacattaacc
    34441 aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt aagctgatat
    34501 tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac ttcgccctgc
    34561 atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg cccattcagg
    34621 cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta
    34681 ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct catcctgcga
    34741 ttgccgatcg caaggtgaat caaccgcgtg ccttaattat ggcaccgacg cgtgaacttg
    34801 ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc tactggcctg aagctgggtc
    34861 tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc ggcgttgaca
    34921 ttctgattgg caccacgggt cgtttaattg actacgccaa gcagaaccac attaacctcg
    34981 gtgccattca ggtagtggtt ctggatgaag ccgatcgcat gtacgatctg ggctttatta
    35041 aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt
    35101 tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg aacaatgccg
    35161 aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa gagcttttct
    35221 acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag
    35281 acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg ggccacctgg
    35341 cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc
    35401 gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc gacgttgccg
    35461 cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta cccgatgact
    35521 gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc ggtcactcta
    35581 tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgaaacc tatattggtc
    35641 actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg ccaaaaccgc
    35701 tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc
    35761 gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg
    35821 caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa gaatgcagcc
    35881 aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct gtatgcagcc
    35941 attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc
    36001 cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat
    36061 gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt
    36121 ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt acgtcttgcc
    36181 gtcaatgcgg gtgattttat tgccaatgcg caggaaatcc tcggttgtcc ggtacaggtg
    36241 atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac aactggcggt
    36301 gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt aaccggcacg
    36361 ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc
    36421 tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc
    36481 gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt
    36541 gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc
    36601 attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg tcggctggag
    36661 gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg
    36721 atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg
    36781 ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc
    36841 cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc gcagcgcgta
    36901 gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct tgaagcaata
    36961 agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttt
    37021 aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc
    37081 cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc
    37141 tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta
    37201 ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca
    37261 ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat
    37321 ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg
    37381 ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg
    37441 attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt
    37501 tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct
    37561 gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg tactgacggc
    37621 gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca
    37681 gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc aaaaaccctt
    37741 cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg tgcagagtca
    37801 tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag
    37861 taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca
    37921 tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg
    37981 ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc agatcgcgca
    38041 ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag tgagtaaagc
    38101 gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg
    38161 cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtcgccg
    38221 ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt
    38281 tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg
    38341 taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca aacactcgcg
    38401 actgatggtt accgcgataa agaatagcgt aatctttgta ctgcgtttta ttgacgaagt
    38461 gatgcgcaat cagttcacca gtgacgcgct cggcttcatg ttcttcgtta ttcgcgctta
    38521 aaactttaag ctctgtgccg taacccagtt cggagaacag acgcttctca aagacgtgcg
    38581 ggttattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag cggtagttct
    38641 gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgtg
    38701 gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg gtaaagcgcg
    38761 cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact
    38821 catccaccag cagatagcga attttgttct gccagcgctc gcggacttct tcattgcgtt
    38881 gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg cacgctttca
    38941 ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg ccaatcgcac
    39001 tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg gtcgaaatca
    39061 actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct ttgagcaacg
    39121 ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc gccgcatact
    39181 cacgcttgat gatatccagc cccagcgtgt ggaaggttga gatcatcagc ccacgcgcct
    39241 ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc ttattggtaa
    39301 aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt
    39361 tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg
    39421 taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc
    39481 ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct
    39541 aaccgattgg ctgcggatgt ttaaatcaga gagggaagac cgaaaccgga gttcatcgtg
    39601 aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt
    39661 actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg aaatactgga
    39721 ttgcaacttt ggtaactatc ctgttagcca gaatggacag ttcagcacca cgtattacga
    39781 tggccgcatt cctcctgaaa tgataaaaa
//
LOCUS       DAJTVE010000021        39469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000021 DAJTVE010000000
VERSION     DAJTVE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OO439_001061"
     CDS             complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OO439_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6315478.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1936..2220
                     /locus_tag="OO439_001062"
     CDS             1936..2220
                     /locus_tag="OO439_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6315479.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2400..3710
                     /gene="fadI"
                     /locus_tag="OO439_001063"
     CDS             2400..3710
                     /gene="fadI"
                     /locus_tag="OO439_001063"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6315480.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3710..5854
                     /gene="fadJ"
                     /locus_tag="OO439_001064"
     CDS             3710..5854
                     /gene="fadJ"
                     /locus_tag="OO439_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6315481.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6057..6542
                     /gene="sixA"
                     /locus_tag="OO439_001065"
     CDS             6057..6542
                     /gene="sixA"
                     /locus_tag="OO439_001065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6315482.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7226..7789
                     /locus_tag="OO439_001066"
     CDS             7226..7789
                     /locus_tag="OO439_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6315483.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7871..10513
                     /gene="yfcU"
                     /locus_tag="OO439_001067"
     CDS             7871..10513
                     /gene="yfcU"
                     /locus_tag="OO439_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6315484.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10533..11284
                     /locus_tag="OO439_001068"
                     /pseudo
     CDS             10533..11284
                     /locus_tag="OO439_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11259..11807
                     /locus_tag="OO439_001069"
     CDS             11259..11807
                     /locus_tag="OO439_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6315485.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11804..12274
                     /locus_tag="OO439_001070"
     CDS             11804..12274
                     /locus_tag="OO439_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6315486.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12271..12795
                     /locus_tag="OO439_001071"
     CDS             12271..12795
                     /locus_tag="OO439_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6315487.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12782..13654
                     /locus_tag="OO439_001072"
     CDS             12782..13654
                     /locus_tag="OO439_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6315488.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OO439_001073"
     CDS             complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OO439_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6315489.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14493..15425
                     /gene="prmB"
                     /locus_tag="OO439_001074"
     CDS             14493..15425
                     /gene="prmB"
                     /locus_tag="OO439_001074"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6315490.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15460..16545
                     /gene="aroC"
                     /locus_tag="OO439_001075"
     CDS             15460..16545
                     /gene="aroC"
                     /locus_tag="OO439_001075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6315491.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16549..17373
                     /gene="mepA"
                     /locus_tag="OO439_001076"
     CDS             16549..17373
                     /gene="mepA"
                     /locus_tag="OO439_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6315492.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17373..18182
                     /locus_tag="OO439_001077"
     CDS             17373..18182
                     /locus_tag="OO439_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6315493.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18182..18730
                     /gene="epmC"
                     /locus_tag="OO439_001078"
     CDS             18182..18730
                     /gene="epmC"
                     /locus_tag="OO439_001078"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6315494.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISLWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18764..19042
                     /locus_tag="OO439_001079"
     CDS             18764..19042
                     /locus_tag="OO439_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6315495.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OO439_001080"
     CDS             complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OO439_001080"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6315496.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21328..22548
                     /gene="fabB"
                     /locus_tag="OO439_001081"
     CDS             21328..22548
                     /gene="fabB"
                     /locus_tag="OO439_001081"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6315497.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22823..24001
                     /locus_tag="OO439_001082"
     CDS             22823..24001
                     /locus_tag="OO439_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315498.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OO439_001083"
     CDS             complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OO439_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6315499.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25092..26228
                     /gene="pdxB"
                     /locus_tag="OO439_001084"
     CDS             25092..26228
                     /gene="pdxB"
                     /locus_tag="OO439_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6315500.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26294..27307
                     /locus_tag="OO439_001085"
     CDS             26294..27307
                     /locus_tag="OO439_001085"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6315501.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27307..28119
                     /gene="truA"
                     /locus_tag="OO439_001086"
     CDS             27307..28119
                     /gene="truA"
                     /locus_tag="OO439_001086"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6315502.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28202..28861
                     /locus_tag="OO439_001087"
     CDS             28202..28861
                     /locus_tag="OO439_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6315503.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29017..29931
                     /gene="accD"
                     /locus_tag="OO439_001088"
     CDS             29017..29931
                     /gene="accD"
                     /locus_tag="OO439_001088"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6315504.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30001..31269
                     /gene="folC"
                     /locus_tag="OO439_001089"
     CDS             30001..31269
                     /gene="folC"
                     /locus_tag="OO439_001089"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6315505.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31259..31921
                     /gene="dedD"
                     /locus_tag="OO439_001090"
     CDS             31259..31921
                     /gene="dedD"
                     /locus_tag="OO439_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6315506.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32180..32668
                     /gene="cvpA"
                     /locus_tag="OO439_001091"
     CDS             32180..32668
                     /gene="cvpA"
                     /locus_tag="OO439_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6315507.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32705..34222
                     /gene="purF"
                     /locus_tag="OO439_001092"
     CDS             32705..34222
                     /gene="purF"
                     /locus_tag="OO439_001092"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6315508.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34317..34886
                     /gene="ubiX"
                     /locus_tag="OO439_001093"
     CDS             34317..34886
                     /gene="ubiX"
                     /locus_tag="OO439_001093"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6315509.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35152..35934
                     /gene="argT"
                     /locus_tag="OO439_001094"
     CDS             35152..35934
                     /gene="argT"
                     /locus_tag="OO439_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6315510.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36155..36937
                     /gene="hisJ"
                     /locus_tag="OO439_001095"
     CDS             36155..36937
                     /gene="hisJ"
                     /locus_tag="OO439_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6315511.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37027..37713
                     /gene="hisQ"
                     /locus_tag="OO439_001096"
     CDS             37027..37713
                     /gene="hisQ"
                     /locus_tag="OO439_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6315512.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37710..38426
                     /locus_tag="OO439_001097"
     CDS             37710..38426
                     /locus_tag="OO439_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6315513.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38434..39207
                     /gene="hisP"
                     /locus_tag="OO439_001098"
     CDS             38434..39207
                     /gene="hisP"
                     /locus_tag="OO439_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6315514.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9347 a         9671 c        10609 g         9842 t
ORIGIN      
        1 aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat atgcctttcc
       61 cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc caacaggctg
      121 tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg ggggagttaa
      181 agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac gcgtagttaa
      241 agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg ccttcgttaa
      301 ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca tctttattaa
      361 atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg gagatagaac
      421 gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag gtccagttat
      481 catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag ccttcatgtt
      541 tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac tgactccagc
      601 tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta taacctgaca
      661 cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg ccacctgtcg
      721 cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg ctgtagttac
      781 ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta tttttatcca
      841 gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc agatgagcga
      901 ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct tgaggagttt
      961 gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg cctgcgaaac
     1021 gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc catgcattat
     1081 ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt gtacccccga
     1141 cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta gaggtaatag
     1201 aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc ggaacccatg
     1261 ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt ccgctgatat
     1321 tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca aacatggtaa
     1381 tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct tcccctgaat
     1441 aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct
     1501 gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg gttttctggc
     1561 tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc tgtaacagga
     1621 gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga gagtataggt
     1681 cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga tctaagaaac
     1741 gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga attttgtttt
     1801 agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta acaactggag
     1861 cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg ggtgcacata
     1921 cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg
     1981 gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg cgtgtttgca
     2041 aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg tagcgtggaa
     2101 tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt gcgcctggat
     2161 atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg tctgcgttaa
     2221 ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct tttctttctc
     2281 ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta aatgtaaaaa
     2341 aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag gggagtgtta
     2401 tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt gttagcggtt
     2461 tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg gttgatttag
     2521 ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa gtgattgaac
     2581 agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg cgtgaaattg
     2641 ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc gcttgcgcta
     2701 ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact atccgagcgg
     2761 ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt aaaaaactgg
     2821 cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg aaactcttct
     2881 ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa tattctactg
     2941 gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc acccgagaac
     3001 agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg tcagacggaa
     3061 aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg cttgtcgaag
     3121 acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc ccggcgtttg
     3181 atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag
     3241 cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg ctggggtatc
     3301 tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc ggtccagcct
     3361 ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg acattgatcg
     3421 atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg ggtagtgaac
     3481 gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat
     3541 ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc ggcgcgcgga
     3601 tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt ttagttaccg
     3661 cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa tggaaatggc
     3721 atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca tcgatgtacc
     3781 ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg ccattattaa
     3841 gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga aaccggacaa
     3901 ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc aagaagcgga
     3961 agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc ccattccggt
     4021 tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc tggcgtgcca
     4081 cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg
     4141 attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagtacagc
     4201 attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct
     4261 ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc tggcgaagaa
     4321 ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg
     4381 tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc aaggcaatta
     4441 tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg gcaccagcag
     4501 cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc aatcgcaagc
     4561 tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta gtgatgcacc
     4621 gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc
     4681 ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca acccgcaggg
     4741 cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca
     4801 tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga cggactatcg
     4861 cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg aattaaaaca
     4921 acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg cttcgaacac
     4981 gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc aagttattgg
     5041 cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc ctcatgcggg
     5101 tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg gcaaaacgcc
     5161 cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc cttacattaa
     5221 tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg ccgcgctagt
     5281 gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa tcgacaccgg
     5341 gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa
     5401 tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta
     5461 tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca tttacccgct
     5521 cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac ggtgtgtgat
     5581 gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta gcgtgcgtga
     5641 cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg gaccgttccg
     5701 ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac tggccacgca
     5761 gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc gtggggaaag
     5821 tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata tgaatccgcg
     5881 ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta cgccattgag
     5941 gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt
     6001 tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg tgcaatatgc
     6061 aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat tcggttcgtc
     6121 ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa
     6181 aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag
     6241 aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct
     6301 gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa ggtgttgctt
     6361 cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag ttatgtccgg
     6421 gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg
     6481 gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca aaagctatct
     6541 gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc tcatcaggca
     6601 gcggatatct gtcaatctca ctactatcga agactctccc catctataaa taaaacactt
     6661 tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca aagttacatg
     6721 ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga taatagagaa
     6781 aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct ggctatagtg
     6841 tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt tcattgtttg
     6901 ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa atacttattc
     6961 cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt aattaaatct
     7021 acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca ctatgcggcc
     7081 ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg ccgtattcaa
     7141 tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt tatattttta
     7201 attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta ttgctgcggc
     7261 aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca acggtactgc
     7321 acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg atcacaaact
     7381 ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca ccaccacgcc
     7441 gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa caatgaccac
     7501 tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca ttttcaatac
     7561 cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc tgggtacctc
     7621 ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta ccaataaagg
     7681 caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg atgcgccaga
     7741 tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag gcataccccc
     7801 atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat aaaaacggat
     7861 agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt ggtgcattgc
     7921 gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc agtttgattc
     7981 ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt ccagccaggg
     8041 atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc cattggcgga
     8101 agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct atgcttgtct
     8161 gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa atctgcaatg
     8221 gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa ttaaggctga
     8281 tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat atacttatcc
     8341 cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg cggactacag
     8401 catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg agatcagcgg
     8461 caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact ggcagactaa
     8521 ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa ctcaaaaaaa
     8581 atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag ccaaactggc
     8641 gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg ttggtggcag
     8701 tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc cagacatttc
     8761 cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg tgatttacga
     8821 aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg tctccggtac
     8881 gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca tcagcaccgc
     8941 ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga tgggccgtcc
     9001 gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag cttcctgggg
     9061 gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact atcagtctgc
     9121 ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg atgttactca
     9181 ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg acggtaactc
     9241 cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta cttttgctgg
     9301 atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg ccagtgacag
     9361 cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata accagaactt
     9421 ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact gggatcgcga
     9481 ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca gtattcgtaa
     9541 tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg acaaaggcat
     9601 gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata acggtaacta
     9661 tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg acgcgactca
     9721 ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct attacagcca
     9781 tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc aatacacctc
     9841 tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg cacttcaccg
     9901 tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg ccgatgttcc
     9961 ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg tcgtttctga
    10021 cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc cggaaaacgc
    10081 cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg gctaccgcaa
    10141 atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag acggcagcca
    10201 tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc ttgtcgatga
    10261 tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg tgttctggag
    10321 tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc tgtttaacgg
    10381 cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg atgacataaa
    10441 gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg tttctgtttc
    10501 agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc tctgccaaat
    10561 tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc aggcttctgt
    10621 aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca gcgtgacgtt
    10681 gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag atgaaaaggg
    10741 taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg attcaatgat
    10801 gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg ccgatcgtga
    10861 aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag ccaacacgct
    10921 gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc tggagaacgt
    10981 cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc aggaatttac
    11041 cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac agaaaaatgg
    11101 caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg caccgctgaa
    11161 tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg ctcggatgcc
    11221 gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga gccgcaaagg
    11281 ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta actgggttac
    11341 tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta agtatgaatt
    11401 tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca gttgagtttg
    11461 gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt gactactcgc
    11521 ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg acaacggtcg
    11581 tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt cgggttcaga
    11641 aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca tttaattttt
    11701 cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca acgttagcgg
    11761 cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac ggatctcgct
    11821 tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc acggttattt
    11881 agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa cttttcagga
    11941 tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt attccattac
    12001 ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga gtggcacact
    12061 aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca ttcagctcaa
    12121 acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa ccgatttacc
    12181 ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg cggactttga
    12241 ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt gttttttatt
    12301 agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt gcactttacc
    12361 ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt ggcagaagta
    12421 cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga gcgagtgcca
    12481 ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa agtaacgttg
    12541 acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc gtcatcagca
    12601 tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat caacaataca
    12661 acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa tacctggtta
    12721 caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt gacggcaacc
    12781 tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa tgctgctcgt
    12841 tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga gtgcgagttt
    12901 tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag cgagcagtgt
    12961 tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg attcttttgc
    13021 gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag ctccaacttt
    13081 ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag atacgaaaat
    13141 ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt atggttgccc
    13201 ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct atacggcccc
    13261 tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata tctcctggag
    13321 tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt caacagtgac
    13381 caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca gtaatttttc
    13441 tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa cctttacctc
    13501 ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg taacggataa
    13561 agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga ttgattcgac
    13621 gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct gatgcgtctt
    13681 atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta ggccggataa
    13741 ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg cggcaaccac
    13801 tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt cggtgcctgg
    13861 tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg cttcaaaata
    13921 tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg acaggcggca
    13981 atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa atccaaaaac
    14041 aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct gacatccggg
    14101 cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc ggagaaataa
    14161 tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct gattttttta
    14221 cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc catcaactgg
    14281 cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta tctgtctcta
    14341 tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt aaacgtattt
    14401 ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc
    14461 gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag
    14521 taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc ttcagcgcgg
    14581 caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta cagctggtgt
    14641 taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg cgtctgacct
    14701 ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag cgcattccgg
    14761 tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc gatgaacgcg
    14821 tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc ggactcatta
    14881 gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc gccattgcct
    14941 gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc gacgcgctgg
    15001 cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt ccgattcgtt
    15061 ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact aacccgccgt
    15121 atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag ccggaactgg
    15181 gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga aacgcggcag
    15241 attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg gtacatctta
    15301 tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggtg
    15361 tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg atttataaag
    15421 attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga
    15481 caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg ctgcatcgtc
    15541 gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga cctcgaccgt
    15601 cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaatt
    15661 ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt gatcgaaaac
    15721 actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc aggccatgct
    15781 gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg acgttcttcc
    15841 gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata tctcgccgag
    15901 aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc gctggaaatc
    15961 aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga caaaatcgac
    16021 gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg cgcgaaagtc
    16081 accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga ccgcctggat
    16141 gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga aattggcgac
    16201 ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac caaagacggt
    16261 ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca gcaaatcatt
    16321 gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac tattaaccgc
    16381 tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt cgggatccgt
    16441 gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct gttacggcag
    16501 cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat gaacaaaacc
    16561 gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac gccgtggcaa
    16621 aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc taatggctgt
    16681 attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat gcgtaccgat
    16741 cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct gagtagccag
    16801 gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc cgctggtggg
    16861 cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat ctttctgcaa
    16921 ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact ggacttagtt
    16981 tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt cagcttgatc
    17041 aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc gattaaacaa
    17101 caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg accctggttc
    17161 cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct ggagtgtgaa
    17221 gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag ctggtttgca
    17281 cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt gccgccttcc
    17341 tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc ctgtttatgg
    17401 tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga tttatcgact
    17461 cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg ggaatgtctc
    17521 ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt tccgccacta
    17581 tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat attgccatga
    17641 cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct gatgtcttac
    17701 ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg atgccaaagc
    17761 tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta attgccggtg
    17821 gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt tacgcgctgg
    17881 ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc aaattactca
    17941 acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa gtgatttggg
    18001 cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt tcacggctgg
    18061 tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg gcggtgatga
    18121 gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg gggatgaact
    18181 aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct gctttgccga
    18241 tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc
    18301 ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg caattcatga
    18361 gatttcgctc tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt tcggctactg
    18421 gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg ttgaagtgaa
    18481 gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta acgtcagttg
    18541 cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc gggtacatgc
    18601 gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct tcattaaagc
    18661 actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt ggccggaagc
    18721 gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg cggagtttga
    18781 atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg atgagttgtt
    18841 tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg aaagtggtga
    18901 tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa aagccattgg
    18961 tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg aaaacctgtt
    19021 tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga tgcgacgctt
    19081 atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc acgccgcatc
    19141 cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc aacaatttcc
    19201 gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc ggaatcggtt
    19261 cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac aaaccgcgag
    19321 aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc gcctcatctt
    19381 tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga acattgccta
    19441 ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct ttatcactga
    19501 catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc tggcgattct
    19561 gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa ctggcaccaa
    19621 tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag cacagcacct
    19681 gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg gcaaccgaat
    19741 acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc gccagtacca
    19801 ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag tcatccttac
    19861 gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt tgcgccagtt
    19921 ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga taagtaatgc
    19981 cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca acggctacag
    20041 ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg cttttctcat
    20101 cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt aattggtcgt
    20161 aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc tcatcgtgtt
    20221 tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc gcgggggctt
    20281 catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc gacaacaacg
    20341 cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc gtacgattaa
    20401 accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa agcatttccc
    20461 gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc aaaccgcggc
    20521 ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc aaccttgcca
    20581 tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca aagccgtcca
    20641 gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc agttcgttaa
    20701 tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa cgatggcaac
    20761 cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc agttccggcc
    20821 agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca aaactaatga
    20881 aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca aatgcctgcc
    20941 atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca aacagaggat
    21001 gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca taacgcgtct
    21061 cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa acaggtgtac
    21121 cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg taagttactc
    21181 gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa tttctctatt
    21241 aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt cgaaacttac
    21301 tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg cctgggcatt
    21361 gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga aggacgttca
    21421 gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt ctggggcaac
    21481 gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat gagcgacgca
    21541 tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct ttctccggaa
    21601 gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg ttccccgcgt
    21661 ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc ggttggcccg
    21721 tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac gccgtttaaa
    21781 attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca ctgtatcggt
    21841 aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg cggcggcgaa
    21901 gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc tactaaatac
    21961 aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg tttcgttatc
    22021 gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc gcgtggtgct
    22081 cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga catggttgct
    22141 ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt tgatacccca
    22201 atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa agagctggca
    22261 gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa agccatgacc
    22321 ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct gatgctggaa
    22381 cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc tgcgggtctg
    22441 aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa cagcttcggc
    22501 ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt cgcagtaggc
    22561 cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc aatgcggcct
    22621 ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg acaatcgcca
    22681 ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt acgcgttaaa
    22741 tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac tcctcgcctt
    22801 gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac gatcttctgc
    22861 caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga ccgtagggct
    22921 gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca ataccatggt
    22981 cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct atgccgggcg
    23041 actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag cttgtggtct
    23101 ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt tcaaatttgc
    23161 cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac tgacaggcgc
    23221 tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga tgtcatggaa
    23281 tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt tgattcatag
    23341 ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac tggcgtgggc
    23401 ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat cgctgtggag
    23461 cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg ttggttttgc
    23521 ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga tggcgggctt
    23581 tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg gctggatgcc
    23641 ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa cggtgggctt
    23701 gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg cgttaaccgg
    23761 agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac gcgtcccctc
    23821 acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg ccctcggcgt
    23881 ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg tatttcttgc
    23941 cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc gtcggggcta
    24001 acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca gcgcgggacg
    24061 cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca gcggttgcat
    24121 atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa tatcctcggc
    24181 ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga tatacgccga
    24241 taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta gtgattccag
    24301 cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca ccagatggtt
    24361 aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca gcatcgattg
    24421 ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca gctgttttag
    24481 cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct gtggttgcgg
    24541 aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct ccggtgagag
    24601 ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg cctgacgatt
    24661 attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag aatggctttt
    24721 ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt gacgcgaaag
    24781 cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca gttctgctct
    24841 ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag tacgttgctg
    24901 actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac ctggtggttg
    24961 cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct gaaatcttcg
    25021 caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg tctcatacag
    25081 gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc gacttattta
    25141 gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct caactggcag
    25201 acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg ctggcaggaa
    25261 aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat gaagcatggt
    25321 tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt gcggtggtgg
    25381 aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca ctgcacgacc
    25441 gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg cgactggaag
    25501 cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg gatgagggtg
    25561 atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc catacgccac
    25621 tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg atccgtagcc
    25681 tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat aatactgcgt
    25741 tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc tgggaaggcg
    25801 aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg catatcgcag
    25861 gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc tatagcaagt
    25921 ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca gagtttggtc
    25981 gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg catttggtgt
    26041 atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg ggtgagttcg
    26101 ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat gtaatttgtg
    26161 atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt catcatccgg
    26221 cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt tgctatttct
    26281 ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa ctggcgctgt
    26341 gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg aaatttatgc
    26401 actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa caatcactgt
    26461 gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg tcgcaggcaa
    26521 agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg tgatcgacag
    26581 cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag taaacccgtt
    26641 tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc tgaccagcca
    26701 gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta tcagtgttac
    26761 cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag cggggcagag
    26821 tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc gtcaactggc
    26881 gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag aacgtcgtat
    26941 cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg caagcgtcgt
    27001 ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac tgcgtccgct
    27061 ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc tctctgaaga
    27121 gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt ctgttggctg
    27181 cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg ccgataacgt
    27241 tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc aggagtatct
    27301 gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg cattgagtac
    27361 gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt gcaggagaag
    27421 ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg cgccgggcgt
    27481 actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac cgcgcagcgc
    27541 aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat cgccgtgcgt
    27601 tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc tcgccgttat
    27661 cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg ggtaacccat
    27721 ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt gctgggcgag
    27781 aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg gcgcaacgtt
    27841 atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa agcgaatgcc
    27901 tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg tgcccacaac
    27961 cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct ggcggcagca
    28021 acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg gtatgatctt
    28081 ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg ctcggaacat
    28141 ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg cataggcaaa
    28201 tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc atctggcaga
    28261 gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc tgttctgcga
    28321 aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg ttgctggtgc
    28381 attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc ttatgttgat
    28441 tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg gtgacaaact
    28501 gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga ctcatcagtt
    28561 ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga tcgtcagaac
    28621 gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg ccgcttataa
    28681 cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt tcttcggcac
    28741 gctccccttc attcagagca accttaagct gatgattgtt ggcattattt ttgtttcaat
    28801 attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg ccgcaaaata
    28861 aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt tatgttttaa
    28921 tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc aaagataaaa
    28981 ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga acgaattaaa
    29041 agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac taagtgtgat
    29101 agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt ctgtccgaag
    29161 tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt agatgaagga
    29221 agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt tcgtgactcc
    29281 aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa agatgcgctg
    29341 gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt cgagttcgcc
    29401 tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc cgttgagcag
    29461 gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc acgtatgcag
    29521 gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc aaaaatgcag
    29581 gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg tgtttctgca
    29641 agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat tggctttgcc
    29701 ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt ccagcgcagt
    29761 gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga aatgcgcctg
    29821 aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc tgaagcgccg
    29881 cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg ataactgata
    29941 agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc agcggatacc
    30001 atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct ttcttatctg
    30061 gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca ggtcgcggcg
    30121 cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac gaatggcaaa
    30181 ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa agtgggcgtc
    30241 tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg gcaggaattg
    30301 ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg tgatatttcc
    30361 ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca ggcacaactt
    30421 gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa tattgtcgat
    30481 gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct gggaccagat
    30541 cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc ggcaattgtc
    30601 ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg tgcactgtta
    30661 caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc gtttagcgat
    30721 gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa tgccgcaaca
    30781 gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat tcgcgacggg
    30841 attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc acgcgttatt
    30901 tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat gaaagcgcta
    30961 ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga tattgccgga
    31021 actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact ggaagggccg
    31081 cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc atttgatagc
    31141 gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac cgtgctggtg
    31201 tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag gagaagcggt
    31261 ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc tgggggtgat
    31321 tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt ttgcggctat
    31381 cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag ccgccaccca
    31441 ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag gtgatgccgc
    31501 agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg aaccggaacc
    31561 tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga aggttgaagc
    31621 accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag cgccaacggg
    31681 taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga atgagattgt
    31741 cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc cagtgcaggg
    31801 taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga aaggttcgct
    31861 gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata cgccgaatta
    31921 atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag tgttagaacc
    31981 gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc cgcaaaggca
    32041 taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc gtgggaagga
    32101 aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg atgacagggc
    32161 gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg tgattgcttt
    32221 ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc tggtgacatg
    32281 gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct ggtttacggg
    32341 ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta tcgctaccct
    32401 gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa cggggttgtc
    32461 aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt tgattgttgc
    32521 cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag actggagcaa
    32581 atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt atctgcaaag
    32641 ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac gaggaaaaag
    32701 acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt cgatttatga
    32761 tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca ccatagatgc
    32821 caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat ttgaagctcg
    32881 ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc ctacggctgg
    32941 cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg gcattacgct
    33001 tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt ttgaagaaaa
    33061 acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct tcgctagcga
    33121 gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg ccattgctgc
    33181 cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg gccacggtat
    33241 ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac gtgatattga
    33301 cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata cgctgggctt
    33361 tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag aagggcagtt
    33421 gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg agtatgtata
    33481 ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc gtgtgaatat
    33541 gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata tcgacgtggt
    33601 gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta ttctgggcaa
    33661 accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta tcatgccggg
    33721 ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg ccgagttccg
    33781 cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt ctgagcagat
    33841 tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg cggcaccgga
    33901 aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac tgatcgctca
    33961 cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga ttttccagga
    34021 tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc agtttgaatg
    34081 ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc tcgatttcct
    34141 cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag tggaaaatct
    34201 cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca ccctgacttg
    34261 caactcccgc cgaaatacta caaagtctgc ctgcaagtct gacagggcaa ctatttatga
    34321 aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg cgcttattac
    34381 aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag gcggcgcgcc
    34441 agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta gctgatgtca
    34501 cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag acgctgggga
    34561 tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc tatactgatg
    34621 gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg gtgctctgcg
    34681 tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg gcagaaatcg
    34741 gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc cttgatgatg
    34801 tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt cctgaagatc
    34861 tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg caattttgca
    34921 accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct gcgattcaac
    34981 cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta acatatttaa
    35041 cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga gcctgcaaac
    35101 acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg tatgaagaag
    35161 tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag ctatgcggcg
    35221 ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc gtcgaaagat
    35281 gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg caaacggatg
    35341 caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc actgaaagcg
    35401 aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca gcaggagatt
    35461 gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa aggttcaccg
    35521 attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca gggatcaacc
    35581 caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt ggcctatgcc
    35641 aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc gttacaagat
    35701 gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt cgcctttgct
    35761 ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg gctacgtaaa
    35821 gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg tcaggacggc
    35881 acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga ctgatacgtc
    35941 gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt gttgcactat
    36001 cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg tctaacgggg
    36061 cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa acctgtaaat
    36121 attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc tatcgctctc
    36181 tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc aaaacatccg
    36241 catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag gcgaactggt
    36301 tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc aatgtacgtt
    36361 tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga ttgacgccat
    36421 catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca ccgacaaact
    36481 gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc cgacagtcga
    36541 gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga cgttcggtaa
    36601 tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg acaacattta
    36661 ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg ctgccagcga
    36721 aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt ctgttaaaga
    36781 tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata acgaactgcg
    36841 cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg agaaattagc
    36901 gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga aagaaagccc
    36961 gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca cgacaggaca
    37021 ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt cacgctggag
    37081 ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc tggcggtaag
    37141 ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct gattcgtggc
    37201 gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc gctaaacacg
    37261 gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc tggtattatc
    37321 actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc ttttatggca
    37381 gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg gcaagtgttt
    37441 cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg caacaactgg
    37501 caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga tgtggtcaaa
    37561 gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat cgtctgtggc
    37621 gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct tgagcgccgc
    37681 tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa tactggaaac
    37741 cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg tggctgctta
    37801 ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt cgtgtctcca
    37861 gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc ggtacgccgc
    37921 tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt gttaagggaa
    37981 ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg gcgctgacgc
    38041 ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg gttccgcatg
    38101 gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat cgttgcatta
    38161 ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg atcctgatgc
    38221 tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa atagcccgcg
    38281 atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg gtgctctatt
    38341 taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc tggttgcagc
    38401 atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt aaacgttatc
    38461 gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact gcaagcgaat
    38521 gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac ctttctgcgc
    38581 tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg ccagacgatc
    38641 aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca actgcgctta
    38701 ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca tatgacggtg
    38761 ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca ggaagcgcgc
    38821 gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca ggggaaatat
    38881 ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc gctggcgatg
    38941 gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga actggtaggc
    39001 gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt ggtagtgact
    39061 cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca tcaggggaaa
    39121 atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc tcgtctgcaa
    39181 cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct gccggagtat
    39241 tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag tccgctaaaa
    39301 atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt gttaatcgtg
    39361 gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa tatcgacaac
    39421 ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccagac
//
LOCUS       DAJTVE010000022        39373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000022 DAJTVE010000000
VERSION     DAJTVE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(314..526)
                     /gene="rpmE"
                     /locus_tag="OO439_001099"
     CDS             complement(314..526)
                     /gene="rpmE"
                     /locus_tag="OO439_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6315515.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            729..2927
                     /gene="priA"
                     /locus_tag="OO439_001100"
     CDS             729..2927
                     /gene="priA"
                     /locus_tag="OO439_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6315516.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3083..4108
                     /gene="cytR"
                     /locus_tag="OO439_001101"
     CDS             3083..4108
                     /gene="cytR"
                     /locus_tag="OO439_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6315517.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4200..5159
                     /gene="ftsN"
                     /locus_tag="OO439_001102"
     CDS             4200..5159
                     /gene="ftsN"
                     /locus_tag="OO439_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6315518.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5252..5782
                     /gene="hslV"
                     /locus_tag="OO439_001103"
     CDS             5252..5782
                     /gene="hslV"
                     /locus_tag="OO439_001103"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6315519.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5792..7123
                     /gene="hslU"
                     /locus_tag="OO439_001104"
     CDS             5792..7123
                     /gene="hslU"
                     /locus_tag="OO439_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6315520.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7190..8116
                     /gene="menA"
                     /locus_tag="OO439_001105"
     CDS             7190..8116
                     /gene="menA"
                     /locus_tag="OO439_001105"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6315521.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8209..8694
                     /gene="rraA"
                     /locus_tag="OO439_001106"
     CDS             8209..8694
                     /gene="rraA"
                     /locus_tag="OO439_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6315522.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8779..9024)
                     /gene="zapB"
                     /locus_tag="OO439_001107"
     CDS             complement(8779..9024)
                     /gene="zapB"
                     /locus_tag="OO439_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6315523.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9450..10295
                     /gene="glpF"
                     /locus_tag="OO439_001108"
     CDS             9450..10295
                     /gene="glpF"
                     /locus_tag="OO439_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6315524.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10318..11829
                     /gene="glpK"
                     /locus_tag="OO439_001109"
     CDS             10318..11829
                     /gene="glpK"
                     /locus_tag="OO439_001109"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6315525.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="OO439_001110"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="OO439_001110"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6315526.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="OO439_001111"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="OO439_001111"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6315527.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO439_001112"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO439_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6315528.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /locus_tag="OO439_001113"
     CDS             complement(14272..14571)
                     /locus_tag="OO439_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6315529.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /locus_tag="OO439_001114"
     CDS             complement(14783..15223)
                     /locus_tag="OO439_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6315530.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /locus_tag="OO439_001115"
     CDS             15324..15923
                     /locus_tag="OO439_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6315531.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="OO439_001116"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="OO439_001116"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6315532.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO439_001117"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO439_001117"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6315533.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO439_001118"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO439_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6315534.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO439_001119"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO439_001119"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6315535.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO439_001120"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO439_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6315536.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21217..21717)
                     /gene="cpxP"
                     /locus_tag="OO439_001121"
     CDS             complement(21217..21717)
                     /gene="cpxP"
                     /locus_tag="OO439_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6315537.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21867..22565
                     /gene="cpxR"
                     /locus_tag="OO439_001122"
     CDS             21867..22565
                     /gene="cpxR"
                     /locus_tag="OO439_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6315538.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22562..23935
                     /gene="cpxA"
                     /locus_tag="OO439_001123"
     CDS             22562..23935
                     /gene="cpxA"
                     /locus_tag="OO439_001123"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6315539.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24041..24715)
                     /gene="yiiM"
                     /locus_tag="OO439_001124"
     CDS             complement(24041..24715)
                     /gene="yiiM"
                     /locus_tag="OO439_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6315540.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24864..25847)
                     /gene="kdgT"
                     /locus_tag="OO439_001125"
     CDS             complement(24864..25847)
                     /gene="kdgT"
                     /locus_tag="OO439_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6315541.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26107..26727)
                     /gene="sodA"
                     /locus_tag="OO439_001126"
     CDS             complement(26107..26727)
                     /gene="sodA"
                     /locus_tag="OO439_001126"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6315542.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27012..28046
                     /gene="rhaT"
                     /locus_tag="OO439_001127"
     CDS             27012..28046
                     /gene="rhaT"
                     /locus_tag="OO439_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6315543.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28043..28891)
                     /gene="rhaR"
                     /locus_tag="OO439_001128"
                     /pseudo
     CDS             complement(28043..28891)
                     /gene="rhaR"
                     /locus_tag="OO439_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28965..29801)
                     /gene="rhaS"
                     /locus_tag="OO439_001129"
     CDS             complement(28965..29801)
                     /gene="rhaS"
                     /locus_tag="OO439_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6315544.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30089..31558
                     /gene="rhaB"
                     /locus_tag="OO439_001130"
     CDS             30089..31558
                     /gene="rhaB"
                     /locus_tag="OO439_001130"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6315545.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31555..32814
                     /gene="rhaA"
                     /locus_tag="OO439_001131"
     CDS             31555..32814
                     /gene="rhaA"
                     /locus_tag="OO439_001131"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6315546.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33056..33880
                     /gene="rhaD"
                     /locus_tag="OO439_001132"
     CDS             33056..33880
                     /gene="rhaD"
                     /locus_tag="OO439_001132"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6315547.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33890..34204
                     /gene="rhaM"
                     /locus_tag="OO439_001133"
     CDS             33890..34204
                     /gene="rhaM"
                     /locus_tag="OO439_001133"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6315548.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34505..34951
                     /locus_tag="OO439_001134"
     CDS             34505..34951
                     /locus_tag="OO439_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6315549.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34962..36413
                     /locus_tag="OO439_001135"
     CDS             34962..36413
                     /locus_tag="OO439_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6315550.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36403..37473
                     /locus_tag="OO439_001136"
     CDS             36403..37473
                     /locus_tag="OO439_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6315551.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37473..39221
                     /locus_tag="OO439_001137"
     CDS             37473..39221
                     /locus_tag="OO439_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6315552.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     LHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39270..>39373)
                     /locus_tag="OO439_001138"
     CDS             complement(39270..>39373)
                     /locus_tag="OO439_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6315553.1"
                     /translation="HLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9570 a        10231 c        10235 g         9337 t
ORIGIN      
        1 gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag gccacgccgg
       61 tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca
      121 tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag
      181 aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt
      241 tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt
      301 cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac
      361 ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca
      421 cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc
      481 agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt
      541 ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag
      601 cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc
      661 aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg
      721 atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact
      781 atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca
      841 aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag ctgccgctta
      901 acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact cactccgtct
      961 ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc
     1021 atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg atgtggtact
     1081 ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcacccaagc
     1141 aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag gttgccacgc
     1201 tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt gatttagcaa
     1261 gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc
     1321 gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt
     1381 ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac
     1441 tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga
     1501 caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt
     1561 ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat ggcgaggcgg
     1621 cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg
     1681 tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc tatcatgccc
     1741 gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa
     1801 cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga
     1861 cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga
     1921 aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca
     1981 acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta ctgtgccacg
     2041 actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc
     2101 aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt
     2161 cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt gaacagacgc
     2221 ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc accagccgca
     2281 aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg attttgatcg
     2341 gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg
     2401 atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gctcagcttt
     2461 acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa
     2521 cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg
     2581 ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga
     2641 ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta
     2701 atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt ccggttccgg
     2761 cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag cacccttccc
     2821 gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca ataccggatt
     2881 cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc gctcacgatg
     2941 cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt
     3001 tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg
     3061 acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa
     3121 agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc
     3181 cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta
     3241 tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat
     3301 tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc
     3361 ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa
     3421 aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag
     3481 gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa
     3541 cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga ccgccgcatt
     3601 tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta tagccggtcc
     3661 cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg
     3721 cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag
     3781 caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag
     3841 cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga
     3901 agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct
     3961 gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga
     4021 tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat
     4081 ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa
     4141 aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag
     4201 tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca
     4261 cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg
     4321 ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag
     4381 agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag
     4441 aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag
     4501 aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc
     4561 ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt
     4621 ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc
     4681 aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc
     4741 agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg
     4801 cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt
     4861 cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg
     4921 agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg
     4981 cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca
     5041 atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc
     5101 tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa
     5161 accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg
     5221 tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa
     5281 cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg
     5341 caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg
     5401 tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg
     5461 ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg
     5521 caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa
     5581 cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc
     5641 ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga
     5701 aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga
     5761 agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt
     5821 cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc
     5881 tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc
     5941 gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct
     6001 ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg
     6061 ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat
     6121 ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg
     6181 tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca
     6241 ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga
     6301 caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc
     6361 aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa
     6421 gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc
     6481 ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca
     6541 cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc
     6601 ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt
     6661 ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt
     6721 ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc caatccgcgt
     6781 tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc
     6841 tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga
     6901 ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat
     6961 cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc
     7021 cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca aacatctgga
     7081 tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt
     7141 catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca
     7201 acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc
     7261 ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact tcgatccgct
     7321 ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta acctcgccaa
     7381 tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg
     7441 catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt
     7501 gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt
     7561 tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg
     7621 caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg
     7681 gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct
     7741 tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata acctgcgtga
     7801 tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa
     7861 cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg cgctgtttaa
     7921 tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt
     7981 gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga
     8041 acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag
     8101 ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta
     8161 catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat
     8221 acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc
     8281 aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac
     8341 aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat
     8401 ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa
     8461 aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga cctagaagag
     8521 ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt
     8581 ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt
     8641 tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga atgataagaa
     8701 aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac
     8761 gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc
     8821 ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg
     8881 atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat
     8941 ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc
     9001 aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa
     9061 atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg
     9121 attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac
     9181 acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta ataaatttgt
     9241 tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa ttgcgcgata
     9301 acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt
     9361 taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac
     9421 aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt
     9481 gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag
     9541 cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg
     9601 gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg
     9661 ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt ccttttatcg
     9721 tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt
     9781 tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa agtgttgatc
     9841 tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag
     9901 ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca
     9961 acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg
    10021 gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga
    10081 aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc
    10141 cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct
    10201 accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa gaaaaggaga
    10261 ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg
    10321 actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta
    10381 atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca
    10441 aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg
    10501 gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg
    10561 aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc
    10621 attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta
    10681 gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc tggcaccaaa
    10741 gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg tggtgaattg
    10801 ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg
    10861 accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac
    10921 gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct
    10981 tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc
    11041 gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg
    11101 gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga gaaagcggtg
    11161 aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga agtgaactat
    11221 gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg cgatgagatg
    11281 aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat
    11341 ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga cccgtatgcg
    11401 cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat acgcgcgacg
    11461 ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc cgactctggt
    11521 atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt cctgatgcag
    11581 ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga agtgactgca
    11641 ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct cgacgagctg
    11701 caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat
    11761 taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga agaacacgac
    11821 gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa acgtgcgggg
    11881 gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg caccattgat
    12361 ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga agtaccagat
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata
    13621 ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg ccccttcatc
    14401 tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctactaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct
    16741 gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agctaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag ataccagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg tgggggaaga
    21181 cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact
    21241 actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat
    21301 tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat
    21361 ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc
    21421 tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt
    21481 ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg
    21541 catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact
    21601 gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt cagcagcgtg
    21661 gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta tgcgcatcat
    21721 ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt caggctgcaa
    21781 acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc tgatgacgta
    21841 atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat gatgaccgag
    21901 agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg attgttgccc
    21961 acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt ttgcttgacg
    22021 taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag acacaccaga
    22081 cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc
    22141 tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc
    22201 gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac aacggttcac
    22261 cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc agcttcgacg
    22321 ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg gcacagcatc
    22381 tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa cgcctgacgc
    22441 ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg ccggatcgta
    22501 aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt
    22561 catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc tggcgctggt
    22621 gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg agcttctgga
    22681 tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc tggcgaacga
    22741 tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata agtgggcacc
    22801 gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag
    22861 cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc atccgcagaa
    22921 gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa
    22981 ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca ttaacttact
    23041 gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc cgctgctgtt
    23101 gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt
    23161 tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg aattccttgc
    23221 cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga cctctcagca
    23281 gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg
    23341 tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta ttgaaaccga
    23401 agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta atcagcaaaa
    23461 aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag tgctggataa
    23521 cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc cgcctgggcc
    23581 gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa
    23641 tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata aagacggtat
    23701 caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg aacagatttt
    23761 ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta caggattggg
    23821 gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg cagaagacag
    23881 cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga gttaaactcc
    23941 gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag gatgcctgat
    24001 gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc cacaaccggc
    24061 gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt
    24121 tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac ggcatatgcc
    24181 atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagcg
    24241 gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc accttacctg
    24301 tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag
    24361 gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata tcgcccatat
    24421 gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca ggcgcaacaa
    24481 acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca cgaggataat
    24541 gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg tcaccttcca
    24601 gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt
    24661 tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt
    24721 ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt
    24781 tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta gttcatccgg
    24841 ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat tttcgctgct
    24901 ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat cgaagtcaca
    24961 atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa cgcaggcacc
    25021 atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt
    25081 ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc
    25141 acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc aatcacagtc
    25201 aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg cactgctttg
    25261 ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac caggaacggt
    25321 aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt gcccagaata
    25381 atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc agcttcttct
    25441 ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat atccatcgcc
    25501 gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc atgttccgga
    25561 ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt taccaccagc
    25621 gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa
    25681 aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt gaatgatcca
    25741 aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg
    25801 agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc catcacctgt
    25861 cggcatattt ttaaggcctg atgcgtaatt cctcaatgga gcatcagtgt tgatggaaac
    25921 gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt gaaacgctgt
    25981 tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt
    26041 tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc agcaggcggc
    26101 aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca ccacgttcca
    26161 gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag catgttccca
    26221 cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac ccatcagcgg
    26281 agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca ccagccatgc
    26341 ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga agttatcaac
    26401 ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg tgcctttttt
    26461 cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc gcagtacggt
    26521 tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc
    26581 aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct gatggtgttt
    26641 ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat cgtaagcata
    26701 cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc ggccgattgt
    26761 taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa aatgattatc
    26821 aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa
    26881 tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt
    26941 aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg aaaaatgatg
    27001 aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg atcggcgcgg
    27061 ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca tgggaaacca
    27121 tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc agcgccctgt
    27181 tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg ctgcctgttt
    27241 ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc atgcgttatc
    27301 tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc ggtacgctga
    27361 tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc ggacgcatga
    27421 cgttgctcgg cgttctggtg gcgctgattg gcgcagggat tgtaactcga gccgggcagt
    27481 tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa gggctggtgc
    27541 tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac gccgcaaaac
    27601 cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct ctgccaagct
    27661 atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt attcgtctgg
    27721 caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg ctgatcattc
    27781 acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc tttttctatg
    27841 cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg ctgcatatga
    27901 gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg aacaatacag
    27961 gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc gccgctaaca
    28021 tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc cactggctgg
    28081 gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct tcaaagccac
    28141 attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat tgcgcatgac
    28201 acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg cgaaattgct
    28261 gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc gcaaagggac
    28321 ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg ctggatgttg
    28381 gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac tgcccgaaca
    28441 gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc tcaagctgac
    28501 cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt ggttgccctg
    28561 cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc agccgctccg
    28621 ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag tgtttatcgt
    28681 cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg ttgagtacat
    28741 gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta tgttcggcaa
    28801 agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat
    28861 catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt
    28921 gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg cagaaagcca
    28981 tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa
    29041 aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct ggcctcgctg
    29101 tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg ctgaggcgtc
    29161 agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga aaattgatcc
    29221 gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc cagaagcaag
    29281 ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg cagcaagagc
    29341 agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc
    29401 tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc
    29461 cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga
    29521 aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt atcggtatgt
    29581 tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt gatggtatag
    29641 ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa
    29701 tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc cacggacgcg
    29761 ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt
    29821 caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac
    29881 cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca
    29941 tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc cctggttgcc
    30001 aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt tttagactgg
    30061 tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg ccgtcgatct
    30121 cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac
    30181 gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt atgtcacctg
    30241 gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt gcgaggaagg
    30301 gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc tgctcgacca
    30361 acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca atggcctaat
    30421 ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg gcatccagtt
    30481 tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac ctgaacttat
    30541 tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc tgaccggcaa
    30601 gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca atagcgacga
    30661 ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg gtcgcccgac
    30721 gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga ttccggtggt
    30781 cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg
    30841 cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc agacgccatt
    30901 taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg aaggtcgcta
    30961 tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc aggagcggca
    31021 aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct gccgcttcat
    31081 catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg aaattcaggc
    31141 tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg cgcgctgcat
    31201 tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc agctacgcgg
    31261 tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc tgctcaacca
    31321 gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag cctcgacgct
    31381 cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg atgatttccg
    31441 tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg acagtgaaat
    31501 tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt gcgcatgacc
    31561 actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt ggggattgat
    31621 gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg ctggcagggc
    31681 gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat tcaggctatt
    31741 ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct ggaacaggct
    31801 atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct ggaatcagac
    31861 acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt tgaatgggcg
    31921 aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc gctaagcgcc
    31981 gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat tgatcactgc
    32041 aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc atcggtgatg
    32101 aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc cccgcgtcag
    32161 cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc gcaccatatc
    32221 gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt tggctccaat
    32281 gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga cgccgggcat
    32341 ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta tgtaccgcag
    32401 ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt gctgctggat
    32461 gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga ccgggtgcat
    32521 atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt cattggtaca
    32581 cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga gctgcgcaag
    32641 ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca gaaatcgttg
    32701 ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc aggtagcgaa
    32761 tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg gtaaacactg
    32821 ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc gtgctgccac
    32881 cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc gtgcggcaac
    32941 ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt tagggtgagg
    33001 ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac agaacatgca
    33061 aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct
    33121 gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg acgccgatat
    33181 cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca gccaacccat
    33241 gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct tccgtaacgt
    33301 ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg gcgcgggcta
    33361 ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc cggctcactt
    33421 cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg
    33481 ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg cggtctttac
    33541 tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat
    33601 tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac aagagatgca
    33661 aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga
    33721 tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta
    33781 ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg
    33841 cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa
    33901 agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc ggcataaccc
    33961 catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta
    34021 cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg
    34081 gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga ccgatgttat
    34141 gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt tttacctgcc
    34201 gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc gatttgaaaa
    34261 attaaacata ttacatctcc cggtaagtta tatttccctg atacattgtg agtaaatcac
    34321 aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc attatgtgca
    34381 ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac cgacatatcg
    34441 cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt atggagtccg
    34501 attaatggca gctcttactg caagctgtat tgacctgaat attcagggca atggcgctta
    34561 ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca ccgactcaca
    34621 ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat ttggttccgg
    34681 tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgtttgtat tattcgcccg
    34741 caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt gctggatctg
    34801 cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca cactgtgtcg
    34861 caaaattatc caccaggatt ttattcatca actgcaacag ggcgacgccg accaggtgct
    34921 tgccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca tccttatgta
    34981 ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg gcggaagccc
    35041 tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa gggtccagtg
    35101 gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc attctcgcta
    35161 ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa gtttatgaga
    35221 ttgccatctc ccaggcgttg aaaaatatcg accagatttt cagcgaatta ccgacaaact
    35281 cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg caaagcggca
    35341 gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc atcggcggtg
    35401 gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat accgatatgt
    35461 cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt ggcttcacct
    35521 ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa ccggctttcg
    35581 ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc acccagtcgg
    35641 gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat tggctatttc gtcttctggt
    35701 tccgcaaagt gcgccttggc aaagcattac aaccgctgct cggttcaatg ctgatcccgt
    35761 tcgttactct gctggttttc ggcgtgctca cttactacgt tatcgggccg gtgatgtccg
    35821 acctcatggg cgggctgctc cacttcctga ataccattcc tccatcaatg aagtttgcgg
    35881 cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg cggccccatc aataaaaccg
    35941 cctggttctt ctgcttctca ctgctggaaa aacacattta tgactggtac gccatcgtcg
    36001 gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc gacgttcatt gcgcccaaac
    36061 tgtttacccg acaggaaaaa gaggctgcca gtagtgccat tgtggtcggt gccacagtcg
    36121 caaccgaacc ggctatacct tacgctctgg cagctccgtt gccgatgatt accgccaata
    36181 cgctggcggg tggcattacc ggcgtgctgg tgatcgcttt cggaatcaaa cgtctggcac
    36241 ctggtttggg tatctttgac ccgctgatcg gcctgatgtc gccggtgggt tcgttttatc
    36301 tggtgctagc gattggcctc gcgctgaaca tttcattcat tatcgtcctg aaaggattgt
    36361 ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt ccatgaacat tgagttactg
    36421 caacagttgt gcgaagccag cgccgtcagc ggcgatgaac aggaagttcg cgacattctg
    36481 ataaacacgc tggaaccttg cgttaatgag atcacctttg atggtctggg cagctttgtt
    36541 gcccgtaagg ggaataaagg tccaaaagtt gccgttgtca ggcatatgga tgaagtcggc
    36601 tttatggtca cccacatcga cgagagcggt tttctgcgct ttaccaccat tggcggctgg
    36661 tggaatcagt caatgctcaa ccaccgggta accatacgca cacacaaggg atttaaaatc
    36721 cctggtgtga ttggttccgt cgcacctcat gcgttaacgg aaaagcaaaa gcaacagccg
    36781 ctgtcatttg atgagatgtt cattgatatt ggcgcgaaca gtcgcgatga ggtggaaaaa
    36841 cgcggtgtgg cgatgggcga ttttattagc ccggaagcca attttgcctg ctggggcgaa
    36901 gataaagtgg tcggcaaggc gctggataac cgcatcggct gcgcaatgat ggctgaacta
    36961 ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg tcggcagcgt ggaagaagaa
    37021 gttgggctac gcggggcaca aacctcggcg gaacacatta aaccggatgt ggtgattgtg
    37081 ctggataccg ctgtcgcagg tgatgttccg ggcattgata acattaaata cccgctgaaa
    37141 ctgggccagg ggccggggct gatgctgttt gacaagcgct acttccccaa ccagaaactg
    37201 gtggcggcgt taaaaagctg tgccgcacat aacgatttac cgctgcaatt ttccaccatg
    37261 aaaaccggtg cgacggatgg cgggcgctac aacgtgatgg gcggcgggcg tccggttgtt
    37321 gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg gcatgatttc aaaagccgat
    37381 tacgatgctc tgctcacgtt gatacgggat tttctgacca ccttaaccgc ggagaaagtc
    37441 aacgcgttta gccagttccg tcaggtggat taatgctaaa cgaacgccag ttaaagattg
    37501 tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct ggcgcaacag actggcgttt
    37561 caggcaggac catcctgcgt gatattgact atctcaactt tacccttaac ggcaaagccc
    37621 gcattttcgc cagtggcagt gcgggctatc agctggaaat cttcgagcgc cgcagctttt
    37681 ttcagttgct gcaaaagcac gataacgacg atcggctgct ggcgctgtta ttactgaata
    37741 cctttactcc ccgtgcgcaa ctcgcctcgg cgcttaattt gccagaaacc tgggtagcag
    37801 agcgtctgcc ccggttaaaa cagcgttatg aacgcacttg ttgcctggcc agtcgccccg
    37861 gtttgggcca tttcattgat gagacagaag agaaacgcgt tatcttgctg gcgaacttgc
    37921 tgcgcaaaga tccgttttta attccgctgg cgggcgtaac acgagacaat tttcagcatt
    37981 tatccacggc ctgcgacaac caacaccgct ggccgctcat gcagggtgat tatctctcca
    38041 gcctgattct ggcgatttac gccctgcgta atcaactgac cgatgagtgg ccgcaatatc
    38101 ccggtaacga gataaaacaa atcgttgaac agagcggcat gtttcttggt gataacgctg
    38161 taagaaccct gacaggtttg atagagaaac agcatcagca agcgcaggta atttcagccg
    38221 atcatgtgct ggggttgctg caaagggtgc caggcatcgc gtcattgaat attattgata
    38281 cgcagctggt tgagaatatt accgggcatt tattacgttg ccttgccgca ccggtgtgga
    38341 ttgccgagca ccgccaaagc agcatgaata acctgaaagc cgcctggcct gcggcctttg
    38401 atatgagtct gcactttatt acgctactgc gtgaacagct cgatattccc cttttcgata
    38461 gcgatctgat cggtttgtat tttgcctgtg cgctggaacg gcatcaaaac gaacgccagc
    38521 cgatcatttt gctctcggac cagaacgcga ttgccactat taatcagctc gccattgagc
    38581 gcgatgtttt acattgtcgg gtgattattg cccgtagctt aagcgagctt attgccattc
    38641 gcgaagagat tgagccgtta ttgatcatta acaacagcca ttatttactg gatgacgcgg
    38701 taaataatta catcaccgta aaaaatatca ttacggctgc cgatatcgaa caaataaaac
    38761 actttctggc gacggcattt attcgccaac agccggaacg ctttttctct gcccccggaa
    38821 gtcttcatta ttcgaatgta cgcggtgaaa gctggcaaca tattacccgg caaatttgtg
    38881 cgcaattagt ggcacaacac catattaccg ccgatgaagc gcaacggatc atcgcccgcg
    38941 aaggcgaagg tgaaaacctg attgttaatc gcctcgcaat cccacattgc tggagcgaac
    39001 aggagcgacg ttttcgtgga ttttttatta ccctcgccca accagttgag gtgaataacg
    39061 aagtcattaa ccatgtcttg atcgcctgcg ccgccgccga tgcgcgtcat gagctaaaaa
    39121 tatttagcta tctggcaagc gtattgtgtc agcatccggc agaagttatt gccgggttaa
    39181 caggatatga ggcatttatg gagttacttc acaaggggtg aactgtgtcg gatgcgccgc
    39241 atccgacact gcattttcat caaaaacgtt taacgcaggc tattatagtc atcaaccatt
    39301 tcgttgtact cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct tgtgtttgca
    39361 tcctgaagat gct
//
LOCUS       DAJTVE010000023        39248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000023 DAJTVE010000000
VERSION     DAJTVE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /locus_tag="OO439_001139"
     CDS             <1..256
                     /locus_tag="OO439_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6315554.1"
                     /translation="EAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKK
                     AQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            350..748
                     /gene="fadM"
                     /locus_tag="OO439_001140"
     CDS             350..748
                     /gene="fadM"
                     /locus_tag="OO439_001140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6315555.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(800..1495)
                     /gene="queC"
                     /locus_tag="OO439_001141"
     CDS             complement(800..1495)
                     /gene="queC"
                     /locus_tag="OO439_001141"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6315556.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1560..3260)
                     /locus_tag="OO439_001142"
     CDS             complement(1560..3260)
                     /locus_tag="OO439_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6315557.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3360..4178
                     /gene="cof"
                     /locus_tag="OO439_001143"
     CDS             3360..4178
                     /gene="cof"
                     /locus_tag="OO439_001143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6315558.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4330..4788
                     /gene="decR"
                     /locus_tag="OO439_001144"
     CDS             4330..4788
                     /gene="decR"
                     /locus_tag="OO439_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6315559.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4818..6590
                     /locus_tag="OO439_001145"
     CDS             4818..6590
                     /locus_tag="OO439_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6315560.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6583..8364
                     /locus_tag="OO439_001146"
     CDS             6583..8364
                     /locus_tag="OO439_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6315561.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8545..8883
                     /gene="glnK"
                     /locus_tag="OO439_001147"
     CDS             8545..8883
                     /gene="glnK"
                     /locus_tag="OO439_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6315562.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8913..10199
                     /gene="amtB"
                     /locus_tag="OO439_001148"
     CDS             8913..10199
                     /gene="amtB"
                     /locus_tag="OO439_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6315563.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10248..11108)
                     /gene="tesB"
                     /locus_tag="OO439_001149"
     CDS             complement(10248..11108)
                     /gene="tesB"
                     /locus_tag="OO439_001149"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6315564.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11326..11898
                     /locus_tag="OO439_001150"
     CDS             11326..11898
                     /locus_tag="OO439_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6315565.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11929..12240)
                     /locus_tag="OO439_001151"
     CDS             complement(11929..12240)
                     /locus_tag="OO439_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6315566.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12403..12499
                     /gene="ffs"
                     /locus_tag="OO439_001152"
     ncRNA           12403..12499
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO439_001152"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12619..12972
                     /locus_tag="OO439_001153"
     CDS             12619..12972
                     /locus_tag="OO439_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6315567.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13014..14562)
                     /gene="pdeB"
                     /locus_tag="OO439_001154"
                     /pseudo
     CDS             complement(13014..14562)
                     /gene="pdeB"
                     /locus_tag="OO439_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14726..15196)
                     /locus_tag="OO439_001155"
     CDS             complement(14726..15196)
                     /locus_tag="OO439_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6315568.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15312..15863)
                     /gene="maa"
                     /locus_tag="OO439_001156"
     CDS             complement(15312..15863)
                     /gene="maa"
                     /locus_tag="OO439_001156"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6315569.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16034..16252)
                     /gene="hha"
                     /locus_tag="OO439_001157"
     CDS             complement(16034..16252)
                     /gene="hha"
                     /locus_tag="OO439_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6315570.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16278..16652)
                     /gene="tomB"
                     /locus_tag="OO439_001158"
     CDS             complement(16278..16652)
                     /gene="tomB"
                     /locus_tag="OO439_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6315571.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17198..20347)
                     /locus_tag="OO439_001159"
     CDS             complement(17198..20347)
                     /locus_tag="OO439_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6315572.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20370..21563)
                     /gene="acrA"
                     /locus_tag="OO439_001160"
     CDS             complement(20370..21563)
                     /gene="acrA"
                     /locus_tag="OO439_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6315573.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21705..22352
                     /gene="acrR"
                     /locus_tag="OO439_001161"
     CDS             21705..22352
                     /gene="acrR"
                     /locus_tag="OO439_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6315574.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22480..25842
                     /gene="mscK"
                     /locus_tag="OO439_001162"
     CDS             22480..25842
                     /gene="mscK"
                     /locus_tag="OO439_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6315575.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26054..26215)
                     /gene="rsmS"
                     /locus_tag="OO439_001163"
     CDS             complement(26054..26215)
                     /gene="rsmS"
                     /locus_tag="OO439_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6315576.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26229..26756)
                     /gene="priC"
                     /locus_tag="OO439_001164"
     CDS             complement(26229..26756)
                     /gene="priC"
                     /locus_tag="OO439_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6315577.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26826..27203
                     /locus_tag="OO439_001165"
     CDS             26826..27203
                     /locus_tag="OO439_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6315578.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27356..27907
                     /gene="apt"
                     /locus_tag="OO439_001166"
     CDS             27356..27907
                     /gene="apt"
                     /locus_tag="OO439_001166"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6315579.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28036..29961
                     /gene="dnaX"
                     /locus_tag="OO439_001167"
     CDS             28036..29961
                     /gene="dnaX"
                     /locus_tag="OO439_001167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6315580.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29294..29358
                     /gene="dnaX"
                     /locus_tag="OO439_001167"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30014..30343
                     /locus_tag="OO439_001168"
     CDS             30014..30343
                     /locus_tag="OO439_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6315581.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30343..30948
                     /gene="recR"
                     /locus_tag="OO439_001169"
     CDS             30343..30948
                     /gene="recR"
                     /locus_tag="OO439_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6315582.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31058..32932
                     /gene="htpG"
                     /locus_tag="OO439_001170"
     CDS             31058..32932
                     /gene="htpG"
                     /locus_tag="OO439_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6315583.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33113..33757
                     /gene="adk"
                     /locus_tag="OO439_001171"
     CDS             33113..33757
                     /gene="adk"
                     /locus_tag="OO439_001171"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6315584.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33889..34851
                     /gene="hemH"
                     /locus_tag="OO439_001172"
     CDS             33889..34851
                     /gene="hemH"
                     /locus_tag="OO439_001172"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6315585.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34848..35807)
                     /gene="aes"
                     /locus_tag="OO439_001173"
     CDS             complement(34848..35807)
                     /gene="aes"
                     /locus_tag="OO439_001173"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6315586.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35959..37263
                     /gene="gsk"
                     /locus_tag="OO439_001174"
     CDS             35959..37263
                     /gene="gsk"
                     /locus_tag="OO439_001174"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6315587.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37396..39072)
                     /locus_tag="OO439_001175"
     CDS             complement(37396..39072)
                     /locus_tag="OO439_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6315588.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9475 a        10017 c        10379 g         9377 t
ORIGIN      
        1 ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg acgaagaagg
       61 cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt
      121 tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac
      181 ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac gtaatctggc
      241 ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg aagaggtcat
      301 accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta tgcaaacaca
      361 aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct
      421 tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat
      481 gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc
      541 ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg
      601 catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat
      661 tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg
      721 cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat
      781 ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg
      841 tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat
      901 gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat
      961 ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg
     1021 gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca
     1081 caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta
     1141 cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac
     1201 gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa
     1261 tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct
     1321 tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat
     1381 gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca
     1441 ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata
     1501 atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt
     1561 cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt
     1621 cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc
     1681 agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc
     1741 tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata tgctggagcg
     1801 tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt atattccggt
     1861 gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag ggtcgtgtcg
     1921 tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc tgccagtgtc
     1981 gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag
     2041 gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca gcctggcagt
     2101 aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa taaaccggat
     2161 tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc
     2221 aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc
     2281 tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc caaatctttt
     2341 tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg acgcaaatgg
     2401 taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa tcgaaaagga
     2461 ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct cgccagccgg
     2521 tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg atgggtgact
     2581 tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat
     2641 aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa
     2701 cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg cgctaaatcg
     2761 ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat ctgcccggcg
     2821 agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag
     2881 tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac
     2941 agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg
     3001 gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt
     3061 aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt
     3121 gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttcg
     3181 ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg atactggtta
     3241 agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact
     3301 tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg gagaaagaaa
     3361 tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag
     3421 gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg
     3481 ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat
     3541 atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacatcgtg
     3601 atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca
     3661 gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc
     3721 aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg
     3781 gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc
     3841 agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca gattgcctcg
     3901 aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatttag
     3961 gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag
     4021 gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc
     4081 cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact
     4141 ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc cagcaccggg
     4201 caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt taaaccgcac
     4261 agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa aattctccgt
     4321 ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca gcaggattgc
     4381 accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc ttgctggaag
     4441 cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct gctggatccg
     4501 gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca tcacagcagc
     4561 gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct ggggttctgg
     4621 cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat gaaacgctac
     4681 gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt cacttccagc
     4741 ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat atccagaatc
     4801 aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc cgtcgggaat
     4861 ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg caactggttc
     4921 cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt actaccgggc
     4981 tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat ctcctgcgtt
     5041 atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa ctgcgtgaag
     5101 attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat cgcacgggtg
     5161 atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc ggagaagggg
     5221 tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg atgtctacgc
     5281 aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg gcgatcatga
     5341 ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg gcgttttcca
     5401 gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa gcctttggtc
     5461 tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc aaaaaaaaca
     5521 tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg attggtatgg
     5581 cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt ttaacgctgg
     5641 gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg ctggcgctgg
     5701 catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt cgtgcgatgc
     5761 tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg cgtggcgaac
     5821 ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg ctggaaaacg
     5881 tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact ggttccggca
     5941 aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg gatattcgct
     6001 ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc ctggcggtgg
     6061 tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg ctgggttgcc
     6121 cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta catgacgata
     6181 ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg atgctttccg
     6241 gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg gaaatcctca
     6301 tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc ctgcataacc
     6361 tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt tcggcactga
     6421 cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt ggcaatcatg
     6481 atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa caactggagg
     6541 cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag ttttagccaa
     6601 ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa accgctgggg
     6661 attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc gctgcttatc
     6721 agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt ggttgcaggg
     6781 ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta cgcgcagtcg
     6841 ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat
     6901 gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca ggtgatttcc
     6961 cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt ggcaactgtc
     7021 ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct cgactggcga
     7081 atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt gatatatcaa
     7141 cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat caacgacggc
     7201 tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca ggcgcgattt
     7261 ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca aaccctgcgc
     7321 ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat tctttgtgga
     7381 ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt gctgtatgcc
     7441 tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac gcaacaggcg
     7501 atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga cggaccgcgc
     7561 cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt cgataacgtg
     7621 tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc tgtgccttcg
     7681 agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct cgccagttta
     7741 ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg tccattaagt
     7801 tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg
     7861 ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga agaacgcgtc
     7921 tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag cgacggtatt
     7981 tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca actgctggca
     8041 ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc aaccgccagc
     8101 attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg tgaacatacc
     8161 acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac cattctggtg
     8221 cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc tgcccaggga
     8281 cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc cagcgtgcgt
     8341 gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa
     8401 cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc ctgctctctt
     8461 tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc agaaccatta
     8521 ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa accattcaag
     8581 ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac cgtcaccgaa
     8641 gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc ggaatacagc
     8701 gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca actcgatgaa
     8761 gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg caaaatcttc
     8821 gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcgctg
     8881 taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata aaaactgggc
     8941 ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg gccgataaag
     9001 ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact attccgggga
     9061 ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg ctgacgcagg
     9121 tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac tcgctggcgt
     9181 ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa aacatcgaac
     9241 tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag ggctcgtttg
     9301 cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc ttctcagctg
     9361 tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg catatggtgt
     9421 ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc acggtggtgc
     9481 atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct
     9541 tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt actgccattc
     9601 tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat gaaatcgcgg
     9661 cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc tggatcttcg
     9721 gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc gcgattgccg
     9781 gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg ttgattatcg
     9841 gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc ttgctgcggg
     9901 tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc ggctgtatca
     9961 tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa ggtgtgacga
    10021 tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc tggtccggtg
    10081 tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt gtgccggaag
    10141 aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat aacgcgtaac
    10201 aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta attgtgatta
    10261 cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt ataaaactca
    10321 ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata cagcagccat
    10381 tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc aatctgaatc
    10441 cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa gttaagatca
    10501 gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct accatttgcg
    10561 cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt atgaaactcc
    10621 accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac tggcggcagc
    10681 aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg cgctggcgcg
    10741 gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga ggccgtcata
    10801 taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa gctgttaccg
    10861 tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg gcgaagaaag
    10921 tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc agcatacaag
    10981 gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc actctggccg
    11041 cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt ttttagcgcc
    11101 tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga gttgagtata
    11161 acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct aattacaggc
    11221 agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc actcttccgg
    11281 cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa actcgtgcac
    11341 atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa aagcgcggat
    11401 attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca acctgctatc
    11461 cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact gccgcctgat
    11521 gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc aaaagtgctg
    11581 gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt tctgcctttt
    11641 aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat taccgtgaat
    11701 gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg cggaactaaa
    11761 gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc cagcggtggt
    11821 gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc ggcagttcca
    11881 gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt agtagttcca
    11941 gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt ctgccagtaa
    12001 tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt ggcgattaac
    12061 cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac cgcccacctg
    12121 gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg tgacatagcc
    12181 ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt ctttttccat
    12241 cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt gaagtcgcat
    12301 tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc agcagtgata
    12361 atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt ctcccgcaac
    12421 gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc
    12481 cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg tcaaaaaatc
    12541 ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc tgcgcagtac
    12601 gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt tcctgccgat
    12661 aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa agagtttggc
    12721 gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt gaccgatttt
    12781 cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat tgaataccct
    12841 tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg gatgaaagag
    12901 ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt cgagtcaatc
    12961 atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa aattcacaaa
    13021 tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta gagccagccc
    13081 tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt ttcgattccc
    13141 tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg cgtgacattt
    13201 ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac atccagatcc
    13261 tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat ttcatggccc
    13321 gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa ctcgcgttca
    13381 gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt cacgcagcaa
    13441 ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa tatgctgctg
    13501 tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa tcagtagcgt
    13561 caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg gtgacaacca
    13621 actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt tgccattggc
    13681 taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag catcctgcag
    13741 ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg ctgccagcag
    13801 gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc ctttctgcaa
    13861 cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg gcaggatatc
    13921 gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc tggtaataat
    13981 tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc caataatggc
    14041 ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg ggtcgatcat
    14101 gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta aatcgttatg
    14161 cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg ggaaggtatc
    14221 gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga tataaaccac
    14281 ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat gggcttcgct
    14341 acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt gtgtcgccac
    14401 cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa taaacgatgt
    14461 ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata ctgaaagaat
    14521 cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat ccttattaac
    14581 acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata aggaggtaga
    14641 gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca agcgcagagc
    14701 tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg agggtaaaaa
    14761 tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt tccttatgaa
    14821 catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt acaaagcgcg
    14881 tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata tcttcatagc
    14941 ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat aatacaacca
    15001 gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg gtggcaaaaa
    15061 aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg atactaaagc
    15121 ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc
    15181 gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg aacaccctat
    15241 tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc ataacagttg
    15301 cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac gacaacgttg
    15361 tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc accaatggtc
    15421 acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat ggtgacgggt
    15481 ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt tgccgtgtag
    15541 atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca gacatcaagc
    15601 atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata gccatagtca
    15661 cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc agcgagaatt
    15721 tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat aagctgacga
    15781 gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc accagcaatc
    15841 atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg gcaacacagt
    15901 aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa gatcacatag
    15961 ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta tagctccgaa
    16021 agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg tcgtatagtt
    16081 tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc gccagttcat
    16141 tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca attgtctggc
    16201 aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac atacttctac
    16261 ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt cgcattgaca
    16321 aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg catattaata
    16381 ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg ctcaatgagc
    16441 ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat atgctcaatc
    16501 agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc atgattgctt
    16561 tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt aagctgtgcg
    16621 atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag cggttgaact
    16681 aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc tcgtttgtta
    16741 gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa attacaatta
    16801 ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag caggtaaaag
    16861 cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat tggtgacact
    16921 aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag aattaccgca
    16981 aataattaag aatagcctct aaatgattat ggattattaa atctattagc gctacttaat
    17041 ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat actggttttc
    17101 gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt atgcataaaa
    17161 aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg acagtatggc
    17221 tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca aagaataccg
    17281 gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca ccggtaccta
    17341 ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata acgccgagga
    17401 taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca tcaagcgtcg
    17461 cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg acgataagta
    17521 tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag taaacgtcat
    17581 tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc ggaacgacca
    17641 gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac aggaatacga
    17701 caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt tcctgatagg
    17761 acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt tgttccatca
    17821 gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt tccatggatg
    17881 gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa gaggagaacg
    17941 ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg atatcatccg
    18001 gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga ccgcggtcga
    18061 taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta atgtcgttga
    18121 tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac tgcggggtat
    18181 cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct gcttctgcaa
    18241 gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg tcaatcagct
    18301 caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta aaggcgaaaa
    18361 ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc gcttcaactt
    18421 tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca ataccggtat
    18481 tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca acgttgttct
    18541 tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta cgttcctgcg
    18601 ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca tctggcaaga
    18661 aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc agatacagca
    18721 ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag tggtgcgtgc
    18781 tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc ccgtgatcgc
    18841 ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc aacgctacca
    18901 gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag atagcaccag
    18961 tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc gcgataccga
    19021 ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc ggcaaacctt
    19081 cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc accaacaagc
    19141 cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca aaggcggcaa
    19201 ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc aacgtcgcgc
    19261 ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct tcgaccagcg
    19321 ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac gggtaaacaa
    19381 ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg attgccgcag
    19441 cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa gccggttggc
    19501 cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc gccacatcac
    19561 gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg aactcttcag
    19621 tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct ttcaccggcg
    19681 gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg gcggtaatga
    19741 catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag atacgcatcg
    19801 cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg atggcatctt
    19861 tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca tcggtgttga
    19921 taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc ccttgctgct
    19981 gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc tgaacctgcg
    20041 cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta cccgtggagt
    20101 cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt tcgataacct
    20161 gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg gagatcgtta
    20221 ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc gccagccccc
    20281 ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg ataaagaaat
    20341 taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg ctgagcaccg
    20401 cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg gacaccagga
    20461 cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc ttctgtcacc
    20521 agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac tttgtcatcc
    20581 gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc ctgttgcggg
    20641 actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa catacccggc
    20701 agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc agtggtctga
    20761 tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat gccgtcactg
    20821 gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt cgccagttcc
    20881 tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat cggatcaagt
    20941 tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc ttccgtcacg
    21001 ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc cagattgatc
    21061 cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg ttgcgcatca
    21121 gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag tttctgataa
    21181 cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag atcacctttc
    21241 gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga gacaccagct
    21301 tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac ttgaggacga
    21361 acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat ctgcagaggt
    21421 tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg ttgggcctgt
    21481 ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac cgccagaggc
    21541 gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa attggtcaat
    21601 ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat ggtacataca
    21661 ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca cgaaaaacca
    21721 aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt cttttctcac
    21781 agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc gttacgcgcg
    21841 gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc tgggaactgt
    21901 cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct ggcgatccac
    21961 tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca gaagaacgac
    22021 gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa atggctgttg
    22081 tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa caaacgttaa
    22141 aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg gcagcaatta
    22201 ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg caatcttttg
    22261 atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat ctcctgtgcc
    22321 ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag gatttttcct
    22381 ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc ggtttgactt
    22441 tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt ccagtattac
    22501 aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt cttgttatgc
    22561 cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa agcggacctg
    22621 caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga caaactggtg
    22681 cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat aaaagaagag
    22741 acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca ggcgaccgcg
    22801 gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat tctgagtacg
    22861 ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt gcaaaacgca
    22921 caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca gcccgaacgc
    22981 gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag tcgtctggat
    23041 gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat gcaggctcag
    23101 caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg gaacaccgtc
    23161 ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc ccgtctggag
    23221 caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt aactgaaaaa
    23281 acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa tccgctggtg
    23341 aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc gactgaaaac
    23401 ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg ggcgctgcaa
    23461 tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct gttgtctcgt
    23521 atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa catgaccaac
    23581 cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga tgcactcttc
    23641 cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt caacagcgaa
    23701 gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga tcaactcaac
    23761 aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca gcagcagtta
    23821 atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg ggtgaacagt
    23881 aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa agatgaattt
    23941 aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt tatcgctttc
    24001 ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct gggctggctg
    24061 aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga tagccagctc
    24121 aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg cctgattatt
    24181 ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact gctatggtcg
    24241 ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa ggtactggag
    24301 aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag ccactggcgt
    24361 cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc tgtggtggca
    24421 gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat tttcttcaac
    24481 ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg tgataaagag
    24541 tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat tgcattgatg
    24601 gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg ttggattgaa
    24661 accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg cggcttaagc
    24721 gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa tctggtgaaa
    24781 gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga acaggttaac
    24841 cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt catgttctgg
    24901 gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac gctctggcat
    24961 tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg cagtctgttg
    25021 tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc tggtttactg
    25081 gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc cattactacc
    25141 atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc gctgggcgtc
    25201 tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg ttttggttta
    25261 caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg tccggtgcgt
    25321 attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat ccgtattcgt
    25381 gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa agcgtttgtt
    25441 accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt gatccgtctc
    25501 ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa ggcggcgact
    25561 gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc gtttggtgcc
    25621 agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg tagtcgtact
    25681 gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat caacattgcc
    25741 tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac ggaagtcaaa
    25801 cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa gggggcgaca
    25861 tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg cggcgtgacc
    25921 gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg tgtaggcctg
    25981 ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga catttagcgg
    26041 ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt tcataatcgc
    26101 gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt tcttccagca
    26161 gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt gacatactct
    26221 ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag cgcatggcgg
    26281 cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg ctgttccacg
    26341 agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg tagccgccgc
    26401 tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg tggcgcactg
    26461 tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag ttgtgccgcc
    26521 agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc atgacaaagc
    26581 gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc acgagtctga
    26641 aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg ggcacaacgc
    26701 tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt tttcacgata
    26761 agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag ctgacaacac
    26821 tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg ctgggtacgc
    26881 tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg gcctggtgct
    26941 ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg tttggcagct
    27001 atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg cgggcgattt
    27061 tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca tgggtgcgca
    27121 tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt ccggtgattg
    27181 atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt gcgtacagcc
    27241 agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac aactgttact
    27301 tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata cacttatgac
    27361 cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc aggactaccc
    27421 aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga aagcttacgc
    27481 tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca aagttgtcgg
    27541 caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg gcgttggctt
    27601 tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa cttacgacct
    27661 ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg gcgacaaagt
    27721 tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg ttaaactgat
    27781 ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt tcgatctcgg
    27841 cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc cgttcccggg
    27901 ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga tgtgcattca
    27961 gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca ccttccagcg
    28021 tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc acaaaccttt
    28081 gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt gtcgttaggg
    28141 cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac ctctatcgcc
    28201 cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc gtgcggcgtg
    28261 tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga aatcgacgcc
    28321 gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca gtacgctccg
    28381 gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc gcgccacagc
    28441 tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt tctgctggcg
    28501 acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca atttcatctc
    28561 aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa cgaagaacat
    28621 atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg cagcctgcga
    28681 gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt ttcaacccag
    28741 gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct ggttgaagcg
    28801 atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc tgcccgtggt
    28861 atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat tgcgatggta
    28921 caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg gatgcgtgaa
    28981 ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct gttgattggt
    29041 cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac gctgctgcgc
    29101 gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg ccagtccttt
    29161 gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca atcagcgccg
    29221 cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc ggcgcgccag
    29281 cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc agccgctacc
    29341 cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac cgatcgcgtt
    29401 caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga agcgtatcgc
    29461 tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc gaaggcgctg
    29521 aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc ggcagaagcc
    29581 attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa actggtcgaa
    29641 caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct gcatttgcgc
    29701 tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga agcgttgagc
    29761 atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc cgcggtgcgt
    29821 acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc gcgcgagtcc
    29881 attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga gctggatgaa
    29941 gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct taacgtgatt
    30001 gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc aagcccagca
    30061 gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag tcaccggcga
    30121 atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc gtcgcgtaga
    30181 gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg tggctgcagc
    30241 attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg cctctgtatc
    30301 ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac cagcccgctg
    30361 ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa gtcggcgcag
    30421 cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt ggcgcaggcg
    30481 ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt caccgaacag
    30541 gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat ctgtgtggtg
    30601 gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg tcgttatttt
    30661 gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat cgggcttgat
    30721 cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc caccaatccc
    30781 acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca atatgacgtg
    30841 gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat ggtcgatggc
    30901 accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc aaacgagagc
    30961 aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc ctcatcctgt
    31021 ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag aaactcgtgg
    31081 ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct attccaataa
    31141 agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca agctgcgttt
    31201 ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg ttcgtgtctc
    31261 tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga tgacccgcga
    31321 cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat tcctcgaatc
    31381 cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg ttggtttcta
    31441 ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag gcgaaaaacc
    31501 agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg ccgacatcac
    31561 caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag acgagttcct
    31621 cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg cgctgccggt
    31681 agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg agaaaatcaa
    31741 caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag agtacaaaga
    31801 gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc acaaccgcgt
    31861 tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc cgtgggatat
    31921 gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt tcatcatgga
    31981 cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga ttgactccag
    32041 cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa cgcgtaacct
    32101 gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga aagacgacgc
    32161 ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag gtccggcgga
    32221 agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta cccataccga
    32281 ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag aagggcagga
    32341 gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc cgcacctgga
    32401 actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg atgagtggat
    32461 gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta aagttgacga
    32521 gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg agaaagcact
    32581 gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag atgtccgtct
    32641 gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg aaatgagcac
    32701 tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga aatacatctt
    32761 cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag atgaagcgaa
    32821 gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac gcggcacgct
    32881 ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct gatgtcatgc
    32941 cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt ccgcaaatta
    33001 tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc tgttggtggt
    33061 atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg caatgcgtat
    33121 cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca tcatggagaa
    33181 atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca aatctggctc
    33241 cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca ccgacgaact
    33301 ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg gtttcctgtt
    33361 ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg gcatcaatgt
    33421 tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta tcgtaggccg
    33481 ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc cgaaagtaga
    33541 aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc aggaagaaac
    33601 cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg gctactactt
    33661 caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca agtcggttgc
    33721 tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca gatctgcttt
    33781 cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt tcttcttcct
    33841 cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat gcgtcagact
    33901 aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta
    33961 aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta
    34021 tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt ggcgaagctg
    34081 tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg ccagcaacag
    34141 caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag ctacggctcg
    34201 ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca tattgtggtg
    34261 ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca
    34321 cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga ttacgccgat
    34381 aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc
    34441 gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc
    34501 gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc actggggatg
    34561 gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc ctggctgatg
    34621 ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tatacaggtg
    34681 atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac
    34741 cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc gcttaatgcc
    34801 acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg
    34861 gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat ccgtgaataa
    34921 tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg ctgatgcgcc
    34981 gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca
    35041 ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta atacggtgac
    35101 tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt
    35161 aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc
    35221 ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc acgcaaccac
    35281 aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac
    35341 atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc
    35401 tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc aatcaccgta
    35461 cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga
    35521 ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg cggacaaaag
    35581 agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt tgccatttct
    35641 ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc
    35701 gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac ggtcttcatt
    35761 tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca aaaactcctt
    35821 tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt
    35881 gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa
    35941 ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttccct
    36001 gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc
    36061 tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga tgatgaattc
    36121 attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga cgtagccgaa
    36181 gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc
    36241 attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt gctgctgggc
    36301 gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg taacacttcc
    36361 agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg
    36421 ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat gaaccagctg
    36481 cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt tctcacctct
    36541 tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa agccattgag
    36601 tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt tgtcattgcc
    36661 gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct tgcgatgaac
    36721 gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg
    36781 ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc
    36841 tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg cgctatagcg
    36901 gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca gaatccgctg
    36961 cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacactaat
    37021 ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat
    37081 cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta
    37141 gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct
    37201 cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt
    37261 taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg catttggctc
    37321 tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca
    37381 tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc
    37441 cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc cacgttcggt
    37501 gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat ccgggttttt
    37561 cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg tcaggatcag
    37621 ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg cattgcccaa
    37681 tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac
    37741 caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta
    37801 acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt cttcgattgc
    37861 ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc
    37921 gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc
    37981 ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc
    38041 aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac caaacagacg
    38101 gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg tcgccagcac
    38161 tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa
    38221 cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc
    38281 agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga cgtcaaacaa
    38341 ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaatagctc
    38401 gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag
    38461 catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat cgactgcaag
    38521 agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag
    38581 cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg
    38641 actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga
    38701 aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc
    38761 catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt
    38821 taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca acagaatgac
    38881 gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg
    38941 tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc
    39001 cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt
    39061 ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac
    39121 caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga
    39181 attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac agaaggcggt
    39241 ttaacaga
//
LOCUS       DAJTVE010000024        39237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000024 DAJTVE010000000
VERSION     DAJTVE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1087)
                     /gene="setA"
                     /locus_tag="OO439_001176"
     CDS             complement(<1..1087)
                     /gene="setA"
                     /locus_tag="OO439_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6315589.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAI"
     gene            complement(1189..1320)
                     /gene="sgrT"
                     /locus_tag="OO439_001177"
     CDS             complement(1189..1320)
                     /gene="sgrT"
                     /locus_tag="OO439_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6315590.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1409..3064
                     /gene="sgrR"
                     /locus_tag="OO439_001178"
     CDS             1409..3064
                     /gene="sgrR"
                     /locus_tag="OO439_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6315591.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3098..3197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3228..4211
                     /gene="thiB"
                     /locus_tag="OO439_001179"
     CDS             3228..4211
                     /gene="thiB"
                     /locus_tag="OO439_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6315592.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4187..5797
                     /gene="thiP"
                     /locus_tag="OO439_001180"
     CDS             4187..5797
                     /gene="thiP"
                     /locus_tag="OO439_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6315593.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5781..6479
                     /gene="thiQ"
                     /locus_tag="OO439_001181"
     CDS             5781..6479
                     /gene="thiQ"
                     /locus_tag="OO439_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6315594.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6549..7313)
                     /locus_tag="OO439_001182"
     CDS             complement(6549..7313)
                     /locus_tag="OO439_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6315595.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7399..8277)
                     /gene="araC"
                     /locus_tag="OO439_001183"
     CDS             complement(7399..8277)
                     /gene="araC"
                     /locus_tag="OO439_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6315596.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8616..10316
                     /gene="araB"
                     /locus_tag="OO439_001184"
     CDS             8616..10316
                     /gene="araB"
                     /locus_tag="OO439_001184"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6315597.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10327..11829
                     /gene="araA"
                     /locus_tag="OO439_001185"
     CDS             10327..11829
                     /gene="araA"
                     /locus_tag="OO439_001185"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6315598.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11998..12693
                     /gene="araD"
                     /locus_tag="OO439_001186"
     CDS             11998..12693
                     /gene="araD"
                     /locus_tag="OO439_001186"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6315599.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12768..15119
                     /gene="polB"
                     /locus_tag="OO439_001187"
     CDS             12768..15119
                     /gene="polB"
                     /locus_tag="OO439_001187"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6315600.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15283..18189
                     /gene="rapA"
                     /locus_tag="OO439_001188"
     CDS             15283..18189
                     /gene="rapA"
                     /locus_tag="OO439_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6315601.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18201..18860
                     /gene="rluA"
                     /locus_tag="OO439_001189"
     CDS             18201..18860
                     /gene="rluA"
                     /locus_tag="OO439_001189"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6315602.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18977..19792)
                     /gene="djlA"
                     /locus_tag="OO439_001190"
     CDS             complement(18977..19792)
                     /gene="djlA"
                     /locus_tag="OO439_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6315603.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20047..22401
                     /gene="lptD"
                     /locus_tag="OO439_001191"
     CDS             20047..22401
                     /gene="lptD"
                     /locus_tag="OO439_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6315604.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22454..23740
                     /gene="surA"
                     /locus_tag="OO439_001192"
     CDS             22454..23740
                     /gene="surA"
                     /locus_tag="OO439_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6315605.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23740..24729
                     /gene="pdxA"
                     /locus_tag="OO439_001193"
     CDS             23740..24729
                     /gene="pdxA"
                     /locus_tag="OO439_001193"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6315606.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24726..25547
                     /gene="rsmA"
                     /locus_tag="OO439_001194"
     CDS             24726..25547
                     /gene="rsmA"
                     /locus_tag="OO439_001194"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6315607.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25550..25927
                     /gene="apaG"
                     /locus_tag="OO439_001195"
     CDS             25550..25927
                     /gene="apaG"
                     /locus_tag="OO439_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6315608.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25934..26776
                     /gene="apaH"
                     /locus_tag="OO439_001196"
     CDS             25934..26776
                     /gene="apaH"
                     /locus_tag="OO439_001196"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6315609.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26854..27333)
                     /gene="folA"
                     /locus_tag="OO439_001197"
     CDS             complement(26854..27333)
                     /gene="folA"
                     /locus_tag="OO439_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6315610.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27525..29387)
                     /gene="kefC"
                     /locus_tag="OO439_001198"
     CDS             complement(27525..29387)
                     /gene="kefC"
                     /locus_tag="OO439_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6315611.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29380..29910)
                     /gene="kefF"
                     /locus_tag="OO439_001199"
     CDS             complement(29380..29910)
                     /gene="kefF"
                     /locus_tag="OO439_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6315612.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30018..31349)
                     /locus_tag="OO439_001200"
                     /pseudo
     CDS             complement(30018..31349)
                     /locus_tag="OO439_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31408..31695)
                     /gene="fixX"
                     /locus_tag="OO439_001201"
     CDS             complement(31408..31695)
                     /gene="fixX"
                     /locus_tag="OO439_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6315613.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31692..32978)
                     /locus_tag="OO439_001202"
     CDS             complement(31692..32978)
                     /locus_tag="OO439_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6315614.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33029..33970)
                     /locus_tag="OO439_001203"
     CDS             complement(33029..33970)
                     /locus_tag="OO439_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6315615.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33985..34755)
                     /locus_tag="OO439_001204"
     CDS             complement(33985..34755)
                     /locus_tag="OO439_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6315616.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35229..36743
                     /gene="caiT"
                     /locus_tag="OO439_001205"
     CDS             35229..36743
                     /gene="caiT"
                     /locus_tag="OO439_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6315617.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36774..37916
                     /gene="caiA"
                     /locus_tag="OO439_001206"
     CDS             36774..37916
                     /gene="caiA"
                     /locus_tag="OO439_001206"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6315618.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38045..39237
                     /gene="caiB"
                     /locus_tag="OO439_001207"
                     /pseudo
     CDS             38045..39237
                     /gene="caiB"
                     /locus_tag="OO439_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9390 a        10784 c        10276 g         8787 t
ORIGIN      
        1 caattgctcc ctgaataacg ccagccagaa ttaccccggt agaaatactg ttagtaaata
       61 aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac
      121 ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc
      181 tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc cgccactgct atgaccatca
      241 ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca
      301 gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aactcgctac
      361 tgatccacaa cggcatatca ataatgtaca tggtgttgca ggtccacatt aacgtcgagg
      421 cgacaaataa catccgtacg ttactatcct gccagccacc ttgcattgat aaagcatttt
      481 ccgacggcag ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga
      541 gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa gccgtaattc aacgccagca
      601 taaaggccaa cggtggaccg ataacccatg ccagagaaag ctgcgcacgc atcaccgagc
      661 taaacatcac cacttctcgc gccgagttat ccgcatattc ccgcgccaga gcaaataact
      721 gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat
      781 aatgacgatt aaatgcaaac aatagcgcat tgccgatagc catcaaacag caaaatataa
      841 tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga
      901 tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct
      961 cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa
     1021 aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc
     1081 agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc
     1141 tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga
     1201 attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa
     1261 ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat
     1321 ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg
     1381 ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat
     1441 ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc
     1501 gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg
     1561 ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta
     1621 taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga
     1681 ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct
     1741 gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg
     1801 taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt
     1861 cagttcccta acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca
     1921 ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca
     1981 tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct
     2041 gccgctctat tcgcatattg cggacattgt gtcgccgacg ccctggacgc tggatatcca
     2101 tctcacgcaa ccggaccgct ggttaccgtt actgctgggg caagttccgg cgatgatcct
     2161 gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat cccatcggca ccggtccgta
     2221 tgcggtgatc cgcaacacca ccaatcaact gaaaattcag gcattcgatg acttcttcgg
     2281 ttaccgggca ttaatcgatg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc
     2341 agctggaggt ctgatgctaa aaggtccaca gggtgaggaa aaagagattg aaagccgcct
     2401 ggaggaaggt tgctactatt tactattcga cagccgcacc catcgcgggg cgaatcagca
     2461 agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga
     2521 acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg
     2581 caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga
     2641 tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt
     2701 cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga
     2761 tatctggctt aacagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt
     2821 gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg
     2881 ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagcaaagc
     2941 aatggtgccg cttatccacc actggctgat cattcagggg caacgcagta tgcgcggcct
     3001 gcgcatgaac accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc
     3061 atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc
     3121 aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg acgaagggat
     3181 ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat
     3241 gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt
     3301 atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg
     3361 aagccgactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca
     3421 accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca
     3481 atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg
     3541 ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat tacggctatt
     3601 tcaccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg
     3661 ttgagagcga tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc
     3721 tgggtctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaga
     3781 aactggcgaa gaaaactgtc acggtcacca aaggctggag cgaagcctat ggcctgtttt
     3841 taaaaggcga aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg
     3901 aagagaagaa agataactac gccgccgcga acttcagcga aggtcactat ctgcaggtgg
     3961 aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt
     4021 ttatggttta tccggctttt cagaatgcga tcccaaccgg caactggatg tatccggtgg
     4081 caaacgtcac gcagcctgcc ggttttgaac aattgaccaa accagcaacc acgctggagt
     4141 tcacgccagc cgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg
     4201 tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc
     4261 ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtagc
     4321 agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct
     4381 ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg
     4441 ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct
     4501 ggttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg
     4561 ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc
     4621 ccatgtgttt tttaatctgc cgatggcgag ccgtttatta cttcaggcac tggaaaacat
     4681 tcccggcgaa caacgtcaac ttgccgccca gcttggaatg cgtggctggc acttctttcg
     4741 tttcgtcgaa tggccgtggt tacggcgaca aatcccgcca gttgctgcgc ttatctttat
     4801 gctctgtttc gccagcttcg ccaccgtact gtcgctaggc ggcggtccgc aagcgaccac
     4861 tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat
     4921 gctggcgctg atccagatga tgtgttgcct tgggctggtg ctgttgagtc agcgattgag
     4981 taaggccatt gcgcccggca ccacgctgct gcaaggctgg cgcgacccgg acgatcgtct
     5041 gcatagccgc atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgttgccacc
     5101 gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaatc
     5161 ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg
     5221 cgtagtgctg accatgatgc tgctatggag cagtcgcgaa cttcgggcgc ggcagaaaat
     5281 gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt
     5341 gctggctacc ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga
     5401 cggcattgtg attttcacca atgcgttaat ggcgatccct tacgcgctga aagtactgga
     5461 aaacccgatg cgcgatatct ccgcccgcta cagcatgttg tgtcagtcgt tgggcattga
     5521 aggctggtca cgcttaaacg tggtggagct gcgcgccctg aaacgcccac tggtgcaggc
     5581 gctggccttt gcatgcgtgc tgtcgattgg tgattttggc gtggtggcgt tgttcggtaa
     5641 cgatgatttc cgcaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca
     5701 ggacggcgcg gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat
     5761 tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt taccaccatt
     5821 tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcgatc ctcgggccaa
     5881 gcggcgcagg taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg
     5941 gttcgctgac tatcgacagc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtat
     6001 cgatgctgtt tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc
     6061 tggggcttaa tccgggattg aagctgaacg cggcgcagca ggagaaaatg cacgctatcg
     6121 cccgccagat ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc
     6181 agcgacagcg cgtggcgtta gcgcgttgtc tggtacgcga gcagccgatt ttattgctcg
     6241 atgaaccgtt ctctgcgctc gatccggcgc tacgccaaga gatgttgacg ctggtgagca
     6301 cgagctgcca gcagcaaaaa atgacgttat tgatggtgtc gcacagtgtg gaagatgccg
     6361 cgcggatcgc cacgcgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaaa
     6421 ccgatgaatt gttaagcggt aaggcgagtg cttcggcgct gttgggaatt acaggttaaa
     6481 agcgcacgga cagtcccctc gcccctctgg ggagaaggga acaaaccagc gccgatgcaa
     6541 actatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg catcaacggg
     6601 tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg cgtcagccac
     6661 aacaaacgac cgcgggacaa ataatgactc aaacggtcag tcgctttacc actgcgccat
     6721 aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag cagcaaccat
     6781 ttaaactcac cgctctgcat cccggcagga atatcgatcg ccgcgcccgc cagaatccct
     6841 ggcaggaagt aaaacggcgg ccacagcagg cagccgataa tattcggcgt gataaatttt
     6901 gccactggca gatccagcat tcccgccacc attggcacca gcggacgcgt cgggccaaca
     6961 aaacgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc agttttatca
     7021 agtagtgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt aaaacgccag
     7081 cccagccaga aggagatcca gtcgcccagc aggcaaccga caatccccgc catccacgcg
     7141 tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat cagcaccgta
     7201 ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa caccaccgcc
     7261 atcaatgaat acacggtgga ttgggtaata aagtgttcca gcaatgcttg cataatgtgc
     7321 ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat
     7381 tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca
     7441 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     7501 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct
     7561 caaaagcagc ttcgcctgac taatgcgttg gtcctcgcgc cagcttaaga cgctaatccc
     7621 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     7681 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     7741 tacacgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     7801 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     7861 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ctgggcgaaa
     7921 gaaacccgta ttggcaaata ttggcggcca gttaagccat tcatgccagt aggcgcgcgg
     7981 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     8041 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgcccctg
     8101 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     8161 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     8221 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     8281 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     8341 tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc ttattaaaag
     8401 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     8461 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     8521 atttttatcc ataagattag cggatcctac ctgacgcttt ttttcgcaac tctctactgt
     8581 ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt ggcctcgatt
     8641 ttggcagtga ttctgtgcga gctttggcgg tggaatgcgc taccggtgaa gagatcgcca
     8701 ccagcgtaga gtggtatccc cgttggcaga aagggcaatt ttgtgatgcc ccgaataacc
     8761 agttccgcca tcatccgcgt gactacattg agtcaatgga agcggcgctg aaaatcgtgc
     8821 ttgcagagct tagcgccgaa cagcgcgcag ctgtggtcgg gattggcgtt gacacaaccg
     8881 gctcgacgcc cgcaccgatt gacgccgacg gtaacgtgct ggcgctgcgc ccagagtttg
     8941 ccgaaaaccc aaacgcgatg ttcgtattgt ggaaagacca cactgcggtt gaagaagcgg
     9001 aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc tatattggcg
     9061 gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc caggacagcg
     9121 ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca gctctgcttt
     9181 ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg cataaatctc
     9241 tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag ctggacccga
     9301 tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc gatattccgg
     9361 tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc gtggtgattt
     9421 ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag cctaacgcac
     9481 tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa cagagcgttg
     9541 gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggtg cctggattta
     9601 tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt ggtcgcgtac
     9661 tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg caaatcaacg
     9721 ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat ccgtctctgg
     9781 atcacctgcc ggtggtgctc gactggttta acggtcgtcg cacgccaaac gctaaccaac
     9841 gtctgaaagg ggtgattacc gatcttaacc tcgctaccaa cgctccgctg ctgttcggcg
     9901 gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt accgatcagg
     9961 ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac caggtcatta
    10021 tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct gaccagtgct
    10081 gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca gacatcccat
    10141 cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc agcgaacagg
    10201 cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc gccgaacaac
    10261 actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttgcgact ctataaggac
    10321 acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag ccagcatctg
    10381 tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt taatgcgctg
    10441 aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac cgctgggcac cacgccggat
    10501 gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg tctggtggtg
    10561 tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat gctcaacaaa
    10621 ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag tatcgatatg
    10681 gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt cattggcgcg
    10741 cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca agcccatgag
    10801 cgtatcggct cctggatgcg ccaggcggtc tctaaacagg atacccgtca tctgaaagtc
    10861 tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa agttgccgca
    10921 cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt gcaggtggtg
    10981 aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag ctgctacacc
    11041 atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga agcggcgcgt
    11101 attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt caccaccacc
    11161 tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagcg tctgatgcag
    11221 cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct tcgcatcatg
    11281 aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta cacctatcac
    11341 ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg cccgtcgatt
    11401 gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg taaggacgat
    11461 cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag cctgattgat
    11521 ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac accgcactcc
    11581 ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct gccaactgct
    11641 tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca tgcgctgaac
    11701 ctcaacgata tgcgccagtt cgccgagatg cacgacattg aaatcacggt gattgataac
    11761 gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta ttacggattt
    11821 cgtcgctaag tagccacatc aggtatgtaa tgcctgatgc gacgctgacg cgtcttatct
    11881 ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca ttgttttccc
    11941 cctcgcccct ttggggagag ggccggggtg aggggaaacg acctctcagg agtcaacatg
    12001 ttagaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa acacaacctg
    12061 gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc
    12121 aaaccttccg gcgtcgatta cagcgtcatg accgctgacg atatggtcgt ggttagcatc
    12181 gaaaccggtg aagtggttga aggtacgaaa aagccctcct ccgacacgcc aactcaccgg
    12241 ctgctctatc aggcattccc ctccattggc ggcattgtgc atacgcactc gcgccacgcc
    12301 accatctggg cgcagtcggg tcagtcgatt ccagcaaccg gcaccaccca cgccgactat
    12361 ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat
    12421 gagtgggaaa ccggtaacgt catcgtagaa acctttgaaa aacagggtat cgatgcagcg
    12481 caaatgcccg gcgttctggt ccattcccac ggcccgtttg catggggcaa aaatgccgaa
    12541 gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt atatggggat attctgccgt
    12601 cagttagcgc cgcagttacc ggatatgcag caaacgctgc tggataaaca ctatctgcgt
    12661 aagcatggcg cgaaggcata ttacgggcag taatgactgt ataaaaccac agccaatcaa
    12721 acgaaaccag gctatactca agcctggttt tttgatggat tttcagcgtg gcgcaggcag
    12781 gttttatctt aacccgacac tggcgggaca ccccgcaagg gacagaagtc tccttctggc
    12841 tggcgacgga caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta
    12901 ttcccgccga tcaggttccc cgcgctcagc atattttgca gggtaaacaa ggctttcgcc
    12961 tgacaccgct ggcgttaaag gattttcacc gccagccggt gtatggcctt tactgtcgcg
    13021 cccatcgcca attgatgaat tacgaaaagc gcctgcgtga aggtggcgtt accgtctacg
    13081 aggccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc tcaccggtgt
    13141 gggtcgaggg tgatatgcac aatggcacta tcgttaatgc ccgtctgaaa ccgcatcccg
    13201 actatcgtcc gccgctcaag tgggtttcta tagatattga aaccacccgc cacggtgagc
    13261 tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg gggccggaga
    13321 atggcgacgc ctcctcgctt gatttcgaac tggaatacgt cgccagccgc ccgcagttgc
    13381 tggaaaaact caacgcctgg tttgccaact acgatcctga tgtgatcatc ggttggaacg
    13441 tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt cttccgctgc
    13501 gtcttgggcg cgataatagc gagctggagt ggcgcgagca cggctttaaa aacggcgtct
    13561 tttttgccca ggctaaaggt cggctaatta tcgacggtat cgaggcgctg aaatccgcgt
    13621 tctggaattt ctcttcattc tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa
    13681 aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc gccgaagata
    13741 aacctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag atcttccaca
    13801 aaactgaaat catgccattt ttactcgaac gagcaacggt gaacggcctg ccggtggatc
    13861 ggcacggcgg ttcggtggca gcgtttggtc atctctattt tccgcgaatg catcgcgctg
    13921 gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacac cagccctggc ggctacgtga
    13981 tggattcacg gccagggctt tatgattcag tgctggtgct ggactataaa agcctgtacc
    14041 cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggttgaaggc atggcgcagc
    14101 ctgatccaga gcacagtacc gaaggttttc tcgatgcctg gttctcacgg gagaaacatt
    14161 gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa cgtcagggta
    14221 ataaaccact ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc gtgcttggca
    14281 ccaccgcctg ccgctttttc gatccgcgcc tggcatcgtc gatcaccatg cgtggtcatc
    14341 agatcatgcg gcaaaccaaa gcattgattg aagcgcaggg ctacgacgtt atctacggag
    14401 ataccgactc gacttttgtc tggctaaaag gtgcacattc ggaagaagag gcggcgaaaa
    14461 tcggtcgtgc actggtgcag cacgttaacg cctggtgggc ggaaacgctg caaaaacaac
    14521 ggctgaccag cgcattagaa ctggagtatg aaactcattt ctgccgtttt cttatgccaa
    14581 ccattcgcgg agctgatacc ggcagtaaaa agcgctacgc cgggctgatt caggagggcg
    14641 ataaacagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg acgccgctgg
    14701 cccagcagtt tcagcaggag ctatacctgc gcatcttccg caacgagcca tatcaggaat
    14761 atgtacgcga aaccatcgac aaactgatgg cgggtgaact ggatgcgcga ctggtttacc
    14821 gtaaacgcct tcgccgtccg ctgagcgagt atcagcgtaa tgtgccgcct catgtacgcg
    14881 ccgctcgcct tgccgatgaa gaaaaccaaa agcgtggtcg ccccttgcaa tatcagaatc
    14941 gcggcaccat taagtacgta tggaccacca acggcccgga gccgctggac taccaacgtt
    15001 caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg gagggaatac
    15061 tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg ctattttgag
    15121 caaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac
    15181 catagcgccc tttccattcc tggacctgaa taacaccact accccataag cacggtagcg
    15241 ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt
    15301 caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat
    15361 gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt
    15421 gattcccccg tgacccgcgt gatgttcaac cctggtgata ctattaccag ccatgacggc
    15481 tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact
    15541 cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg
    15601 gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt
    15661 gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc
    15721 ctgcgcggtc agcgtaccag cctgatcccg catcagctca acatcgctca tgatgttggt
    15781 cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa
    15841 gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt gctaattatc
    15901 gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc
    15961 tttgcgctat ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttc
    16021 gacaccgaac agctggtgat ttgctcgctg gattttgccc gtcgtagcaa acagcgcctg
    16081 gaacatctct gtgaagccga atgggacctg ctggtggtcg atgaagcgca tcacctggtg
    16141 tggagcgaag atgcgccaag ccgtgaatat caggccattg aacaactggc agagcacgtg
    16201 ccgggcgttc tgctgctgac cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc
    16261 cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg cgcagttcgt tgaagagcag
    16321 aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc
    16381 aatgacgaac tgaacatgct cggcgagatg atcggcgagc aggatatcga gccgctgttg
    16441 caggcagcaa acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg
    16501 ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa
    16561 ggattcccga aacgcgagct gcacaccatt aagctgccgc tgccgacgca gtatcagacg
    16621 gctattaaag tctccggcat tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat
    16681 atgctctacc cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac
    16741 ttcgatccgc gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg
    16801 ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt
    16861 gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct
    16921 gccgcctggt ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt
    16981 tctgaaggac gtaacttcca gttcgccagc cacatggtga tgtttgacct gccattcaac
    17041 ccggatctac tggagcagcg tattggtcgt ctggatcgta tcggccaggc gcacgatatt
    17101 cagatccatg tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac
    17161 gaaggtctgg atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta
    17221 tacaacgatc tgattaacta tctggcttca ccggttcaaa ccgaaggctt tgacgatctg
    17281 atcaaaaact gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac
    17341 cgcctgctgg aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt
    17401 gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc
    17461 ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg
    17521 ctggtgccgg acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa
    17581 gtggcgctgg cgcgtgaaga tgcacagttt attacctggg agcatccgct gatccgcaac
    17641 ggtctggatc tgatcctttc tggcgatact ggtagcagca cgatttcact attgaaaaac
    17701 aaagcgttgc cggtaggtac gctgttggtg gaactgattt atgtcgttga agcccaggct
    17761 ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat
    17821 aaaaacggca acaacctggc ggcgcaggta gagtttgaaa cctttaaccg tcagcttaac
    17881 gcggttaacc gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct
    17941 atccttcagc tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgccgca
    18001 cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtatggaagc gctgcgcgca
    18061 gtgaacccga acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta
    18121 atggaaagcc tggatcaggc aggctggcgt ctggatgccc tgcgtttgat cgttgtaacg
    18181 catcagtaac ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt
    18241 tggttatcct gtatcaggat gaccacatta tggtggttaa caagccgagc ggtttgttgt
    18301 ctgtgccggg gcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt
    18361 atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg
    18421 cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa
    18481 agcagtatgt agcccgcgtc tgggggcatc catcccctgc ggaaggtctg gtggatctgc
    18541 cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac
    18601 ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt
    18661 taaaaccgat taccgggcgt tcgcatcagc tgcgtgtgca tatgctggcg ctgggtcatc
    18721 cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt
    18781 tgctacatgc agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta
    18841 aagcgccagc ggatttttaa tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc
    18901 acgccgcatc cggcaattgt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag
    18961 gcctacagtt taaaacttat ttaaaccctt tctgctgctt tatcagctca tatgcctgct
    19021 gaatttcctg cgctttctgc ttcgccatct ccatcatctc aggcggcaaa cctttcgcca
    19081 ccagcttatc gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg
    19141 catcatccgt cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct
    19201 gcgcttgctg ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac
    19261 cgccctgcat catgcgcaaa aactggtcaa actgggcgcg ggagatccct aattcttcag
    19321 caatgacata cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct
    19381 gaatctggat ttccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa
    19441 actggcgcat cttttcgcgc agcgggtaat tatctgattt tcccacccgg aacgcgtttt
    19501 gcgccgcagt acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat
    19561 gaatatcagc ctccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag
    19621 tggtggcaaa aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc
    19681 tacgggcttt atcaaacata tggccaatta acaggcctaa cactacgccc caaaagccgc
    19741 cgcccatcag taaggccacg gccacgccaa tgatttttcc ccaatactgc atatattccc
    19801 caaatcgaca cacggatatc agggctatct cccacaatat aaaggtgctt ttaccgtttt
    19861 ccggctgcgg tcaattgtgg gacatcgcct ataatttgca ttatcatacc tgtcattcaa
    19921 tgtcgtgcct aacaccactg acgctattcg gacaggatta acactagcgt agagatgacg
    19981 agtacgttag tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat
    20041 caacgtatga aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat
    20101 agtcaacagg gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat
    20161 gaccgtcctc tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac
    20221 gcgaaagggg actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt
    20281 aacagccgtc tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg
    20341 gagccggtac gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc
    20401 ctcaaagggc cgaaaggctg ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt
    20461 gattaccaga tggtgggtcg ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc
    20521 gaaaaccgct ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac
    20581 acctggagcg tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgcggagatc
    20641 tggaacgccc gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg
    20701 ccggtgggtg acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc
    20761 aactactttg agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc
    20821 atcacgccgc attatatgca tcgtcgtggc aacatcatgt gggagaacga attccgctac
    20881 ctctcccagg cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat
    20941 gaagatgaac acccgaacga tgacagttca cgtcgttggt tgttctactg gcaacactcc
    21001 ggggtcatgg atcaagtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc
    21061 tacttcaatg atttcgataa caagtacggt tccagtactg acggctacgc aacgcaaaaa
    21121 ttcagcgttg gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag
    21181 gtctttagcg agcagaacac cagtagctac tcggcagagc cgcagttaga cgttaactac
    21241 taccagaatg atgttggtcc gtttgatacg cgtatttacg gccaggcagt gcactttgtt
    21301 aacaccagag acgacatgcc tgaagcaacc cgtgttcacc tggaaccgac catcaatttg
    21361 ccgctctcta ataactgggg cagcatcaat accgaagcga agttgctggc aacccattat
    21421 cagcaaacca atcttgactg gtataactcc agaaacacga ccaagctgga cgaatccgtt
    21481 aaccgcgtta tgccgcaatt caaagttgac ggcaaaatgg tctttgaacg cgatatggaa
    21541 atgctggctc cgggttatac ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg
    21601 tatcgcgatc agagcgacat ctataactac gactcgtctc tactgcaatc tgactactct
    21661 ggcctgttcc gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaggtg
    21721 acgaccggtg tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc
    21781 gttggtcaaa tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag
    21841 aatgacgaca aaacgggctc actggtgtgg gcaggcgata cttactggcg tatctccgag
    21901 cgttggggat tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcgaccagt
    21961 aactccagca ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccgttac
    22021 gccagcccgg aatatattca ggctacgctg cctaagtact attccactgc tgagcaatat
    22081 aagaatggta tttcgcaggt aggtgctgtc gccagctggc caattgccga tcgttggtcc
    22141 attgttgggg cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt
    22201 gtgcaataca gctcctgctg ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt
    22261 tgggataacg ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt
    22321 cgcggcctga gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg
    22381 ccgtatcaaa acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg
    22441 aaatggaaaa agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa
    22501 taccagtttc gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt
    22561 cgtgctggaa agcgacgttg atggattaat gcagtcggta aaactgaacg ctgcgcaggc
    22621 aaggcagcag cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat
    22681 ggatcaaatc atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct
    22741 ggatcaggcg attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag
    22801 ccgtctggct tacgatggtc tgaactacaa cacctatcgt aaccagatcc gcaaagagat
    22861 gattatctct gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccgcagga
    22921 agtcgaatcc ctggcgcagc aggtgggtaa ccaaaacgac gccagcactg agctgaacct
    22981 gagccacatc ctgatcccgc tgccggaaaa cccgacttct gatcaggtga acgaagcgga
    23041 aagccaggcg cgcgccattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc
    23101 gattgctcat tctgccgacc agcaggcgct gaacggcggc cagatgggct ggggccgtat
    23161 tcaggagttg ccgggtatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt
    23221 tggcccgatt cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga
    23281 aagcaaaaat atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc
    23341 gatcatgact gacgaacagg cccgtgtgaa actggaacag attgctgccg atatcaagag
    23401 tggtaaaacg acttttgctg ccgcagcgaa agagttctct caggatccag gctctgctaa
    23461 ccagggcggt gatctcggct gggctacacc agatattttc gatccggcct tccgtgacgc
    23521 cctgacccgc ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca
    23581 tttaatcgaa ctgctggata cccgtaatgt cgataaaacc gacgctgcgc agaaggatcg
    23641 tgcataccgc atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga
    23701 acaacgcgcc agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt
    23761 gtgatcactc ccggcgagcc cgccgggatt ggcccggact tagttgtcca gcttgcacag
    23821 cgtgagtggc cggtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca
    23881 gcgatgctcg gtttgccgct caccctccgc ccttattccc ccaactcccc tgcacaaccg
    23941 caaactgcgg gcacattaac gctacttcct gtcgcgctac gcgaatctgt cactgcgggg
    24001 cagttagcga ttgaaaatgg gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc
    24061 tgtctgaacg gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac
    24121 gacgcaggta ttccgtttac tggtcatacc gagtttttcg aagagcgctc gcaggcgaaa
    24181 aaagtggtga tgatgctggc gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta
    24241 ccgctgcgcg atatcgcaga tgctatcacc cctgcacttt tgcacgaagt gattgctatt
    24301 ttgcatcacg atttgcgtac aaaatttggt attgccgaac cgcgcattct ggtctgcggg
    24361 ctgaatccgc acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt
    24421 ccggtgctca acgagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat
    24481 accctgtttc agccgaaata tctcgataac gccgacgccg tgctggcgat gtaccacgat
    24541 cagggtcttc ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc
    24601 ctgcccttta ttcgcacatc agtggaccac ggcaccgcgc ttgaactggc gggacgtggc
    24661 gaagccgatg tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac
    24721 acccaatgaa taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact
    24781 ttctcaacga tcagttcgtg atcgacagca ttgtttctgc cattaacccg caaaagggcc
    24841 aagcaatggt cgaaatcggc cccggtctgg cggcattgac cgagccggtc ggcgaacgtc
    24901 tggaccagct gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc
    24961 cattcttagg cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg
    25021 aactggccga gaaaatgggt cagccgctgc gtgttttcgg caacctgcct tataacatct
    25081 ccacgccgtt gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta
    25141 tgttgcaaaa agaggtggtg aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc
    25201 gattaagcgt catggcgcaa tactattgca acgtgatccc agtgctggaa gtaccgccgt
    25261 cagcctttac accaccaccc aaagtggatt ccgccgtcgt gcgcctggtt cctcatgcaa
    25321 cgatgcctca cccggttaaa gatgttcgcg tgttgagccg catcaccact gaagccttta
    25381 accagcgtcg taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa
    25441 cgggaatggg gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc
    25501 agatggcgaa ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc
    25561 gccccgagtg tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa
    25621 tgaacgttac gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca
    25681 gttgttgggg cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg
    25741 cgaaggagtg gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag
    25801 cggtgcaatc attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga
    25861 aaatggcgtc cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat
    25921 tcattaaaag aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact
    25981 gatcgcattg ctgcataaag tagaatttac ccctgggaaa gataccctct ggctgacggg
    26041 cgatctggtc gcgcgcgggc cgggttcgct ggatgttctg cgctatgtga aatccttagg
    26101 cgacagcgta cgtctggtgc tgggcaatca cgatctgcat ctgctggcgg tatttgccgg
    26161 gatcagccgc aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga
    26221 cgagctgctt aactggctgc gtcgccagcc tttgctgcaa atcgacgaag agaaaaagtt
    26281 ggtgatggcc cacgcaggga tcacgccgca gtgggatcta cagaccgcca aagagtgcgc
    26341 gcgcgatgta gaagcggtgc tatcgagtga ctcctatccc ttctttctcg acgctatgta
    26401 cggcgatatg ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat
    26461 caccaacgct tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag
    26521 caaagaatcg ccggaagagg cccctgcccc actgaaaccg tggtttgcga ttcctggccc
    26581 tgtcgctgaa gaatacagca tcgcctttgg tcactgggca tcgctggagg gcaaaggtac
    26641 gccggaaggt atatacgcgc tggataccgg ctgctgctgg ggtggtacat taacctgcct
    26701 gcgctgggaa gataaacagt attttgtcca gccgtcgaac cggcataagg atttgggcga
    26761 agcggcggcg tcttaaacac agcctgatat aggaaggccg gataagacgc gaccggcgtc
    26821 gcatccggca ctagccgtaa attctataca aaattaccgc cgctccagaa tctcaaagca
    26881 atagctgtga gagttctgcg catcagcatc gtggaattcg ctgaataccg attcccagtc
    26941 atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag
    27001 atacagtttc tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat
    27061 gatttctggt acgtcaccac acgccgcgat ggcttcatcc accgacttca cccacgttac
    27121 gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg
    27181 accgattgat tcccaagtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt
    27241 aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg
    27301 atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaaattgtc
    27361 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27421 tttatattct gctggcgagt ccacgctctc tccctggact cgccgcatta caatgaaaca
    27481 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattagga tgagggtttc
    27541 gtttccggtt catccgccat gttgccggta tgtttacctt cttcggttcc ctgccagcca
    27601 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtaa tgatctcact taacatcgcg
    27661 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcgt tctcaaccat tgccatctct
    27721 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27781 cccagaccta aactttccag tgccagacgc ccggttttca gcgcaccttc gaaggtttca
    27841 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtggtcgac atcgcgggcg
    27901 cgggcaataa tctgcaaatg cgggaagtgt tctttcacca tctctgtcag ttgcaggttg
    27961 gtttgcggat cgtcgatggc gttaatcagc acttccgctt tcgccgctcc cgcagattcc
    28021 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcaaggtt
    28081 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28141 cgtccggtga tctgcccaaa acgaccgaat ccggcaatga ttacccgcgg ctgttcttcg
    28201 tcgatctcat cagcttcacg cgcctcttca gtagaagatt gctcaaggcg attgaggatc
    28261 accagcagaa tcggcgttgc cgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28321 cactccggtt ccagcacatt cgccatctgc gccgtgccaa ataccacaaa ggcaaactca
    28381 ctgccctgcc ctaacaacac cgcaaaccaa cgacgctgtt tatttggcac ttgcaacggt
    28441 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28501 atgcgcaatg ggttttcaag cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28561 aacagcccca acagcaaacc tttaaatggt tcgatatcgc tctccagcgc atgacggtat
    28621 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28681 tcttccagca gcaaaccaaa gccaaacacg aggaataacg ccacggcact aaacacttcc
    28741 cgcaagccag agcgggctac aaaacgcagc gccggacgcg tgacatagcg ccccagcaat
    28801 accaccagca ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28861 gtcgaggcac tgctcgttgc cagtagcgga atcatcgcca ccagcgggat cgccgcgata
    28921 tcctggaaca gcagcaccgc aaaggcactg cgacccattt gcgtcaccat cagattgcgt
    28981 tcattcatcg cctgcatggc aatcgccgta gaggagagcg ccagcgtcat gccgatcaat
    29041 tccgcgacct gccagtgcag cccaagtaac atgcagaaca gccccagcag gccgccgcaa
    29101 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29161 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29221 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29281 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgct
    29341 gccgaaccga gataaatcag cgcctgaatc agcgtatggc tatccatgat gggcctcctg
    29401 ccattccagc agacgttgct tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca
    29461 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29521 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29581 ttccccgccg ccggtcgtca ccgcccacag caaatatttg ccatgcagcg ccttgccgcc
    29641 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29701 aatgctgtac cactgcatcg gatgctgcca gacgatcaga tcggcgcgag acagcgcctc
    29761 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29821 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29881 atacggatgc gcataaatta taagaatcat aggtagcccg cgtgattacg cctttgtttt
    29941 cccacaaagt gtagtcatat attcacgcgg cttatagtca ataatattga ttcagaaaag
    30001 agagaaatct gaacaattta ccccattctc tgcccgcgga tcgtcatatt gctggtttgc
    30061 gccagtgaca tccctcgagt ctccggggca aacgctacgg aaatcaacaa gccaaacagc
    30121 gagatacccg cctccattag catcgtgtta ctgataccgt aattattgat aaagatcggc
    30181 agtgcccagg tcgaaacaat ggtgccaata cggcttaagg acataatcac gcccacggca
    30241 gaggcgcgga tatctgtcgg gaagagttca ttaggataga gccactgcaa attacccggc
    30301 ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat ccccatatcc
    30361 gggatcagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc aatcaacaat
    30421 ggacgccgtc cggcagtgtt taaccacagc atcggcggaa tacagccgag cataaagaac
    30481 aggctaatca ccacgttccc cagcgccgcg tttttgccaa cacccaatcc caacaaacca
    30541 acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca ggtccagatg
    30601 gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt
    30661 tgctacggct gctcttcatc gaaagccacc ggttcgccaa acagttttat catcatctct
    30721 tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattcagg cagttcgaat
    30781 cgaccaatca aaatcaacaa acaggggatc gccgcgctac ccagcatcca gcgccagccg
    30841 ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc gacataccac
    30901 atggcggcga taaagctgat ggaaaatgcc cgctgacggg tactggagaa ctcggtgatc
    30961 attgaggtgg cgatgggata atctgcaccg atgacaatgc cgataagtac ccgcatcacc
    31021 aacagttcga cgggggatga aacaaacatc gtcgccaccg atatcacgcc gatggcgatg
    31081 atatcaatga ggaacatttt gcgccgtcct actttatcgg aaatatagcc aaacagcgat
    31141 gtgccaacga acagcccggc gagcgttccc gcgcccaaca agccaatcca gtcagcgtcc
    31201 agttttagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag tacataacca
    31261 tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaattga ggagaatttg
    31321 agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc aatgagagtc
    31381 agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac caaaggtgcc
    31441 gcgcgggtat tcccactgtt ccagcgccga ccccagcccc agaatgcgac aggtgccgca
    31501 ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct tgtacagacc
    31561 tgcggggcac gctttcacca gcagctccag ggcctgttta tcggcatcgg cctttacaac
    31621 aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga cgtccacatt
    31681 gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag attgatgaag
    31741 cccactttct tgccgtggcg gaggattttc ttgcgcatca gttccggcgc actgccgtca
    31801 atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct aaacatgcgt
    31861 gggttatcaa ggaaggccgg tagtttctgg tacatacgca tatcgcgcag cgggccactc
    31921 tcaagatgct gacgatattc cgccagtttt tgcttactga aatcgtcgct tttcatcgct
    31981 gaaagcacgg tttttgctgc ggcttccccg gcggcaatcg ccagatccat accgcgaatg
    32041 gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac gccgtcacca
    32101 accaactccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga atattccacc
    32161 agcttgccgc ccgcgatcag cggtgccact gccggatgct gtttgaaatc ttccagcatt
    32221 tgcggcaccg atttttttgc gtcatgcaga tgatgcagac cacaaaccag ccccagcgac
    32281 agggtatttt cattggtata aaggaagccg ccgcccatca ggccatcggt gggtgatccc
    32341 gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc gattaccgac
    32401 ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt aacgcgtttt
    32461 gccatcccca gcttttcggc aaggatggag ttcactccat cagcaaggat caccgttttc
    32521 gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg ctgtacgagg
    32581 ttatcgacgc ggatcccggt aattaactgc gcgcccgctt cttcggcctg ctccatcagc
    32641 caggcatcaa atttactgcg caaaacggag taagaacgct gggatggcga ggtttcgtca
    32701 ccgttgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag tttttcatgg
    32761 gtgatcaggc gttctaccgg ggcggattcg gcgaaaccag gaataatgtg ttccaggctg
    32821 tgggcataga gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc acgctcgata
    32881 actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc ggcaagccca
    32941 gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctccctg tcagcggcag
    33001 ggccaaaatg cagccctgcc agagtggatc aacgcgctaa agctgcggtc agcgccggaa
    33061 ggatcttcac ggcgtcacca acaatgccgt aatccgcgta ctggaagatc ggcgcatttt
    33121 tatctttatt gatggcgaaa atggtttgcg atgcattagc accaaccatg tgctggatct
    33181 gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg gagataccga
    33241 catagcgttc gtgctccatc catttttcgt tttccgccac cggacgagaa caggccaact
    33301 ccgcacctat cgccttgcaa agctgttctg ccagcgcaat gttctctttg ctgccaatac
    33361 cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg ctctggcgcg
    33421 cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg gtgtgcgttt
    33481 cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg atggtcagta
    33541 ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accaccgtaa accatatgtt
    33601 tcactgttgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac accgccgctt
    33661 taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt ggcagcagca
    33721 ccaggccgtc tgcgccgtgc tggcgaatag tgtcagccat gacaccggcg taatcttcga
    33781 tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg agctggattg
    33841 cctgtgcgcc gtcggcatca ttgaggacaa aggtgttgat ttgattagct aaagcctgcg
    33901 caccgttcat cagttccggc agacgagaag gggtatcgct gaatacccag acttgagaaa
    33961 acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag caaatgcggc
    34021 gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgttcgcgct gtttcggcgc
    34081 ggcaacctgt tgttctgacc aggctgcctc tgcgttaaaa ccaatatccg ccgccgacca
    34141 tacctggacg ggctttttcg ccgcgccgag aatggctttc atcgaaggaa tttgtgggga
    34201 attgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg tttcggtttc
    34261 atcttccagt tcgcgctcaa cggtgagggt atctgccgtc agggagataa ttttgctgac
    34321 gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct gctgggcata
    34381 aaggtcggaa gaaccatcgc cacagaggat caaatcaaag cctgctttct gggcggctgc
    34441 agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat caatcaccac
    34501 aatcagttca tccgggccgc gcgatagtac atctttacgc cctttggcgt tggtcagggc
    34561 tttaccgccc acacttaagg ccgtcacctg cgcctctgct gcctgttgct ttagctggca
    34621 agccgcttca atagcgttca gatcgtattg gcttattttg gcatcggctt tgctgaagtc
    34681 taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc acttatagca
    34741 agtaataatc ttcattgcat ctccagaaat catgaaggaa acatcgttca tatcggtggt
    34801 gttaattaca gatatcacgc cctttaattt ggaaataaaa ctttaatcac caatattgaa
    34861 aatgtcacac gcagataaat ccaactttca atattgttaa gttcctcacc aatattgaaa
    34921 acatggcgta gaaaaaagaa attttcaata ttgctttatg gatcaccaat attgaaagtc
    34981 agatgctatt tttttactca caacacaaca taacaaactg attaataatc aaaattaacg
    35041 agaaaagcgc cctctattca cccgaaaaaa aaatgtgata acaatcacag aatgcagctt
    35101 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatc
    35161 aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa atattcgttt tcacagtgga
    35221 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35281 cgttaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg
    35341 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35401 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35461 gtttaggtaa cgaaccacct gaatttagta ccgccagttg gatctttatg atgttcgcct
    35521 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35581 ccccgccgtt tggcttagaa ccgaactcga caggggcgaa agagttgggg ctggcttaca
    35641 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35701 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35761 tggtaggtga aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg
    35821 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35881 gtatgcaatg gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct
    35941 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    36001 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg
    36061 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36121 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36181 tgttctactg ggcatggtgg gtgatttatg ctatccagat gagtatcttc ctcgcccgca
    36241 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt
    36301 caacctggat cctgtggact gtactcggta gtaacactct gctgttgata gataaaaaca
    36361 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc attgaaacct
    36421 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36481 ccaccgttac actggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag
    36541 tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36601 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36661 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36721 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36781 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36841 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36901 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36961 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    37021 caacctatgt gctgtaccag ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac
    37081 aagagcagat cgacaaaatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37141 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37201 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagcagc gcctacaccc
    37261 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37321 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc
    37381 gtatggatag ctgctgtgaa atcacctttg acgacgtgga actggacgag aaagacatgt
    37441 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37501 tggtagccct caccaactac ggtacggcga tgtgcgcttt tgaagatgcg gcgcgctacg
    37561 ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37621 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37681 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37741 atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37801 gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt agaccgcgtc tccgggggat
    37861 ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct gaagcaatac cgctaagtcc
    37921 gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc tacgggagca cgaatgtagg
    37981 ccggataaag cgtttacgcc gcatccggca gtcatgcact acataaattt ttcaggagct
    38041 aataatggat catctaccca tgccgaaatt cgggccgttg gccggattgc gcgttgtctt
    38101 ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg ttcgcagaat ggggtgcgga
    38161 agttatctgg atcgagaacg tcgcctaggc cgacaccatt cgcgttcaac cgaactaccc
    38221 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38281 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gcaaaggtcc
    38341 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38401 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38461 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38521 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38581 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38641 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38701 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38761 taaatgcgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38821 ctttaaagat attgacctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38881 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38941 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    39001 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39061 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39121 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39181 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaa
//
LOCUS       DAJTVE010000025        38329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000025 DAJTVE010000000
VERSION     DAJTVE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            61..1425
                     /locus_tag="OO439_001208"
     CDS             61..1425
                     /locus_tag="OO439_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315619.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1553..2044)
                     /gene="yajQ"
                     /locus_tag="OO439_001209"
     CDS             complement(1553..2044)
                     /gene="yajQ"
                     /locus_tag="OO439_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6315620.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2212..3123
                     /gene="panE"
                     /locus_tag="OO439_001210"
     CDS             2212..3123
                     /gene="panE"
                     /locus_tag="OO439_001210"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6315621.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3086..3676
                     /gene="yajL"
                     /locus_tag="OO439_001211"
     CDS             3086..3676
                     /gene="yajL"
                     /locus_tag="OO439_001211"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6315622.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3730..5178)
                     /gene="thiI"
                     /locus_tag="OO439_001212"
     CDS             complement(3730..5178)
                     /gene="thiI"
                     /locus_tag="OO439_001212"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6315623.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5384..5626
                     /gene="xseB"
                     /locus_tag="OO439_001213"
     CDS             5384..5626
                     /gene="xseB"
                     /locus_tag="OO439_001213"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6315624.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5626..6525
                     /gene="ispA"
                     /locus_tag="OO439_001214"
     CDS             5626..6525
                     /gene="ispA"
                     /locus_tag="OO439_001214"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6315625.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6550..8412
                     /gene="dxs"
                     /locus_tag="OO439_001215"
     CDS             6550..8412
                     /gene="dxs"
                     /locus_tag="OO439_001215"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6315626.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8467..9441
                     /gene="yajO"
                     /locus_tag="OO439_001216"
     CDS             8467..9441
                     /gene="yajO"
                     /locus_tag="OO439_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6315627.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9495..10013)
                     /gene="pgpA"
                     /locus_tag="OO439_001217"
     CDS             complement(9495..10013)
                     /gene="pgpA"
                     /locus_tag="OO439_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6315628.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9991..10968)
                     /gene="thiL"
                     /locus_tag="OO439_001218"
     CDS             complement(9991..10968)
                     /gene="thiL"
                     /locus_tag="OO439_001218"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6315629.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11046..11465)
                     /gene="nusB"
                     /locus_tag="OO439_001219"
     CDS             complement(11046..11465)
                     /gene="nusB"
                     /locus_tag="OO439_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6315630.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11485..11955)
                     /gene="ribE"
                     /locus_tag="OO439_001220"
     CDS             complement(11485..11955)
                     /gene="ribE"
                     /locus_tag="OO439_001220"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6315631.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12044..13147)
                     /gene="ribD"
                     /locus_tag="OO439_001221"
     CDS             complement(12044..13147)
                     /gene="ribD"
                     /locus_tag="OO439_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6315632.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13151..13600)
                     /gene="nrdR"
                     /locus_tag="OO439_001222"
     CDS             complement(13151..13600)
                     /gene="nrdR"
                     /locus_tag="OO439_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6315633.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13751..14290
                     /locus_tag="OO439_001223"
     CDS             13751..14290
                     /locus_tag="OO439_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6315634.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14589..15473
                     /gene="tsx"
                     /locus_tag="OO439_001224"
     CDS             14589..15473
                     /gene="tsx"
                     /locus_tag="OO439_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6315635.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15650..15997)
                     /locus_tag="OO439_001225"
     CDS             complement(15650..15997)
                     /locus_tag="OO439_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6315636.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16126..17097)
                     /gene="secF"
                     /locus_tag="OO439_001226"
     CDS             complement(16126..17097)
                     /gene="secF"
                     /locus_tag="OO439_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6315637.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17108..18955)
                     /gene="secD"
                     /locus_tag="OO439_001227"
     CDS             complement(17108..18955)
                     /gene="secD"
                     /locus_tag="OO439_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6315638.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18983..19315)
                     /gene="yajC"
                     /locus_tag="OO439_001228"
     CDS             complement(18983..19315)
                     /gene="yajC"
                     /locus_tag="OO439_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6315639.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19338..20465)
                     /gene="tgt"
                     /locus_tag="OO439_001229"
     CDS             complement(19338..20465)
                     /gene="tgt"
                     /locus_tag="OO439_001229"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6315640.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20521..21591)
                     /gene="queA"
                     /locus_tag="OO439_001230"
     CDS             complement(20521..21591)
                     /gene="queA"
                     /locus_tag="OO439_001230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6315641.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21684..22265
                     /gene="acpH"
                     /locus_tag="OO439_001231"
     CDS             21684..22265
                     /gene="acpH"
                     /locus_tag="OO439_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6315642.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22270..24087)
                     /gene="malZ"
                     /locus_tag="OO439_001232"
     CDS             complement(22270..24087)
                     /gene="malZ"
                     /locus_tag="OO439_001232"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6315643.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24243..25616)
                     /gene="proY"
                     /locus_tag="OO439_001233"
     CDS             complement(24243..25616)
                     /gene="proY"
                     /locus_tag="OO439_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6315644.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25692..27011)
                     /gene="brnQ"
                     /locus_tag="OO439_001234"
     CDS             complement(25692..27011)
                     /gene="brnQ"
                     /locus_tag="OO439_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6315645.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27418..28713)
                     /gene="phoR"
                     /locus_tag="OO439_001235"
     CDS             complement(27418..28713)
                     /gene="phoR"
                     /locus_tag="OO439_001235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6315646.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28771..29460)
                     /gene="phoB"
                     /locus_tag="OO439_001236"
     CDS             complement(28771..29460)
                     /gene="phoB"
                     /locus_tag="OO439_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6315647.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29650..30852
                     /gene="sbcD"
                     /locus_tag="OO439_001237"
     CDS             29650..30852
                     /gene="sbcD"
                     /locus_tag="OO439_001237"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6315648.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30849..33992
                     /gene="sbcC"
                     /locus_tag="OO439_001238"
     CDS             30849..33992
                     /gene="sbcC"
                     /locus_tag="OO439_001238"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6315649.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34118..35302
                     /gene="araJ"
                     /locus_tag="OO439_001239"
     CDS             34118..35302
                     /gene="araJ"
                     /locus_tag="OO439_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6315650.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35445..36353)
                     /gene="mak"
                     /locus_tag="OO439_001240"
     CDS             complement(35445..36353)
                     /gene="mak"
                     /locus_tag="OO439_001240"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6315651.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36478..37389
                     /gene="rdgC"
                     /locus_tag="OO439_001241"
     CDS             36478..37389
                     /gene="rdgC"
                     /locus_tag="OO439_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6315652.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37467..37922)
                     /locus_tag="OO439_001242"
     CDS             complement(37467..37922)
                     /locus_tag="OO439_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315653.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37919..38254)
                     /locus_tag="OO439_001243"
                     /pseudo
     CDS             complement(37919..38254)
                     /locus_tag="OO439_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9468 a        10313 c         9725 g         8823 t
ORIGIN      
        1 tctgtttacc caacatgcgc tccgcactac actatgtggg cattttttta aggggtggta
       61 atgaacgatt ataaaatgac gccaggtgag aggcgcgcga cctggggttt agggaccgta
      121 ttctcgttgc gcatgctggg catgtttatg gttctgccgg ttctgaccac gtacggcatg
      181 gctctgcaag gtgccagcga agcattaatc ggtattgcca ttggtattta tggtctgact
      241 caggccgttt ttcagattcc gtttggcctg ctttccgacc gcattggtcg caaaccatta
      301 attgtcggtg ggctggcggt gtttgccgcc ggtagcgtta tcgctgcgct ttccgactcc
      361 atctggggaa ttattctggg ccgggcgcta caaggctctg gagcaattgc cgccgccgtt
      421 atggcgctgc tttccgatct cacgcgcgaa caaaaccgca ccaaagcgat ggcgtttatc
      481 ggcgtgagct ttggcattac ctttgccatt gcgatggtgc ttggcccgat catcactcac
      541 aaacttgggc tgcacgcgct gttctggatg atcgctattc tggcaacaac cggcattgcg
      601 ttgaccattt gggttgtgcc caacagtagc actcacgtac ttaatcgtga gtccggaatg
      661 gtgaaaggca gtttcagtaa agtgctggcg gaaccgcggc tgctgaaact caactttggc
      721 attatgtgtc tgcacatgct tctgatgtct acgtttgttg ccctgcccgg acagctagct
      781 gatgcggggt tcccggcggc tgaacactgg aaggtctatc tggcgacgat gttaatcgcc
      841 tttggctcga tcgtgccttt cattatctac gctgaagtta agcgcaaaat gaagcaagtc
      901 tttgtcttct gcgtcgggtt gatcgtggtt gcggaaattg tgttgtggaa cgcacaaacg
      961 cagttctggc aactggtggt cggcgtgcag cttttctttg tggcgtttaa tttgatggaa
     1021 gcccttctgc cctcacttat cagtaaagag tcgccagcag gttacaaagg tacggcgatg
     1081 ggtgtctact ccaccagcca gtttcttggc gtggcgattg gcggttcgct gggcggctgg
     1141 attgacggca tgtttgacgg tcagggggta tttctcgctg gcgcaatgct ggccgcagtg
     1201 tggctggcag tcgccagtac catgaaagaa ccgccgtatg tcagcagttt gcgcattgaa
     1261 atcccggcgg acattgccgc aaacgaagcg ttaaaagtgc gtttgctgga aactgaaggc
     1321 gtcaaagaag tgttgattgc agaagaagag cattcagctt atgtgaaaat cgacagcaaa
     1381 gtgacaaatc gctttgaggt tgaacaggca attcgtcagg cataaaaaac gcggcgaatt
     1441 gcctgctgcg ctacgcttac caggcctaca aagttcttga gttacaaacc gttgtaggcc
     1501 gaatcaggcg ttcatgccgc atctgacatg aacaaaacgc acattgtcgc gattaatcgc
     1561 ggaagttttt gaactggaac ggctggccga gatcgccacc acgtaccatc gccatgacag
     1621 cctgcaaatc atcacgagat ttgccagtca cacggatctc atcgccctga atttgcgcct
     1681 gcactttcag tttgctgtct ttaatcatct tgacgatttt cttctgggtc gcgctctcaa
     1741 tgccctgttt cagtttcgct tccacaaacc aggttttacc gctatgaacg atattttccg
     1801 gtacatccag tgaactgcct tcaatgccgc gcttcagcag cttggcacgc agaatatcca
     1861 gcaactgatt gacctggaaa tcggactcgc tcaacacttt gatggttttg ctggcgtcgt
     1921 tcagctcaaa tgaggcttca acattacgga agtcaaaacg ggactccact tcgcggctcg
     1981 cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc agagacaata tcgaaagatg
     2041 gcatcttttc ctctcccttc atttttgatg cgaagcataa tacccgcaaa gttaaataac
     2101 cgacagtcct gcaccaggac gtatccctta attgtgcata gtcacaacag catgcacaat
     2161 taagggatag ccttattgaa cacccggagt ggttgcgggt gaggaggaac aatgaaaatt
     2221 accgtattgg gatgcggtgc cttagggcaa ttatggctta cagcactttg taaacagggt
     2281 cacgaagttc agggctggct gcgcgtaccg caaccttatt gtagtgtgaa tctggttgag
     2341 actgatggtt cgatatttaa cgaatcgctg accgccaacg atcccgattt tcttgccacc
     2401 agcgatctgc tcctggtgac gctgaaagca tggcaggttt ccgatgccgt caaaagcctc
     2461 gcgtccacac tgcctgtaac cacgccaata ctgttaattc acaacggcat gggcaccatc
     2521 gaagagttgc aaaacattca gcagccatta ctgatgggca ccaccaccca tgccgcccgc
     2581 cgcgacggca atgtcattat tcatgtggca aacggaatca cgcatattgg tccggcacgg
     2641 caacaggacg gcgattacag ctatctggcg gatattttgc aaaccgtgtt gcctgacgtt
     2701 gcctggcata acaatattcg cgccgagctg tggcgcaagc tggccgtcaa ctgtgtgatt
     2761 aatccactga ccgccatctg gaattgcccg aatggtgaat tacgtcatca tccgcaagaa
     2821 attatgcaga tatgcgaaga agtcgcggca gtgatcgagc gcgaagggca tcatacttca
     2881 gcagaagatt tgcgtgatta cgtgatgcag gtgattgatg ccacagcgga aaatatctcg
     2941 tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa tcgactatat caatggtttt
     3001 ctcttacgcc gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa
     3061 atggtaaaaa gaaaggagag tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg
     3121 tagtgaagag actgaagccg tcaccactat cgatctgctg gttcgcggcg gtatcaaagt
     3181 caccactgcc agcgtcgcca gcgatggcaa cctggccatt acctgctcgc gcggcgtgaa
     3241 gctgctggcg gatgcgccgc tggtcgaggt ggctgatggc gaatatgacg tgatcgtact
     3301 gcctggtggc attaaaggcg cagagtgttt tcgcgatagt accctgctgg ttgaaaccgt
     3361 taaacagttc caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt
     3421 gctggtaccg cacgatatct tcccgattgg caatatgacc ggcttcccga cgctgaaaga
     3481 caaaattccc gccgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt
     3541 gctgaccagc caggggccgg gtacggctat tgattttggt ctgaaaatta tcgacctgct
     3601 ggttgggcgc gagaaaaccc atgaagtggc atcacaactg gtgatggcgg cagggattta
     3661 taattattac gagtagtgtc ggatgcggca aacgtcgcat ctgaccagat gcgacgtaaa
     3721 aaccacaaat tatgggcgat acaccttcac attgttaaag ccctgctcgc gcagatagag
     3781 cgcctgcaga cggctcatca ccccgcgctc acaccacagt agccaggttc tgttctggtc
     3841 gagatcgcca aatttggtgc tcagtttata gaacggcaga gaaaccacgt cgataccttc
     3901 aaccttcagc ggcttatctt cctgctcatc gacagaacgg atatcgagga tcacgtcgtt
     3961 cgggccaaag ccattgacgg tttccacttc caccacttcc tgctcggtct gctgggcgat
     4021 ttcgcggata tcaacgttat tcgcttcctc aaccacttta tcgagaatac tgaagtcgaa
     4081 cttctcttct tccgcttcaa tcttcgattt aaccgctttc accgtcgggc ttttggagat
     4141 cacgccacaa tattctggca tcgtgcgagc aaagtcttca gtgccaatct ggcgggccag
     4201 gttgatgatg tgctctttgt cgtaagaaat cagcggacgc aggatcagcg tatcagagac
     4261 gttatcaatc aggcgcaggt tggtcagcgt ctgactggac acctggccga gcgcttcgcc
     4321 ggtgaccagc gcctgtacgc cgtaacgttc agccacttta gacgcggcac gcaccatcat
     4381 acgtttgagg ataacgccca tctgaccgtc gtcgattttc tcgagaattt ccccgacgac
     4441 cggttcgaaa ttaatggcga caaaacgcac gcggtgggag ctgccaaagc ggttccacag
     4501 ataatgcgcc acctgacgca cgccaatttc atgcgctgcg ccgccgaggt taaagaagca
     4561 gtaatgcacg cggcagccgc gacgcatcaa catataactg gaaacaccgg agtcgaaacc
     4621 accggaaatg agcgacagca catcttcctg ggtgccaatc gggaaaccgc caataccttc
     4681 gtagcggcct ttaatcagca gaagacgatc gtcttccact tccagatgga cagttacatc
     4741 cgggttagtc agcttcacgc gcgcggattc aatatgctga tttaaaccgc cgccgacgta
     4801 acgttccaca tcaatcgagc taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa
     4861 ggttttgcct tccagctgat cgcgatattg aaccaacgct ttctcgaaaa tatcgtgcat
     4921 gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg atacccggaa tacgggtcag
     4981 ggcgtcgcga atagccagac gctgtttttc atcttttgcg cgaacttcga tgttatccca
     5041 gtggcggacg acagcgagcg tctcatcata gtgctttaaa acgttacgaa tgttcccggt
     5101 aaggattttt ataaagcgca agcgcacaga ttggcttttg atggtgattt ccgggaacaa
     5161 tttaatgata aacttcatgg cggcaatggt tcgttggcaa gccttaagcg acttgtatag
     5221 ggaaaaatac agcagcccac acctgcggct gcatccaggc gcggaagtat accactaaca
     5281 tcgctttgct gcgcacatca ccttaccatt gcgcgttatt tgctatttgc cctgagtccg
     5341 ttaccatgac ggggcgaaaa atattgagag tcagacattc attatgccga agaaaaatga
     5401 ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa cagattgtaa cccgtctgga
     5461 aagtggcgac ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg tgcagctggc
     5521 acgtcagggg caggccaaat tacaacaagc cgaacagcgc gtacaaattc tgctgtctga
     5581 caatgaagac acctctctac ccccttttac accggacaat gagtaatgga ctttccgcag
     5641 caacttgaag cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg
     5701 ccctttcaga acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag
     5761 cgcctgcgac cttttctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg
     5821 ctggacgcac ccgctgccgc cgttgagtgt atccacgctt actcattaat tcatgatgat
     5881 ttaccggcga tggatgatga cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt
     5941 ggcgaagcaa acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgatttta
     6001 agcgatgccg atatgccgga agtgtcggac cgcgacagaa tttcgatgat ttcagaactg
     6061 gcaagcgcca gcggtattgc cggaatgtgc ggtggtcagg cattagattt agatgcggaa
     6121 ggcaaacacg tacctctgga cgcactcgag cgtattcatc gtcataaaac cggcgcattg
     6181 attcgcgccg ccgttcgcct tggtgcatta agcgccggag ataaagggcg tcgtgctctg
     6241 ccagtactcg acaagtacgc agagagcatc ggccttgcct tccaggttca ggatgacatc
     6301 ctggatgtgg tgggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt
     6361 ggtaaaagta cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat
     6421 ctgatcgacg atgcccgcca gtcgctgaaa caactggctg aacaatcact cgatacctcg
     6481 gcactggaag cgctagcgga ctacatcatc cagcgtaata aataaacaat aagtattaat
     6541 aggcccctga tgagttttga tattgccaaa tacccgaccc tggcactggt cgactccacc
     6601 caggagttac gactgttgcc gaaagagagt ttaccgaaac tctgcgacga actgcgccgc
     6661 tatttactcg acagcgtgag ccgttccagc gggcacttcg cctccgggct gggcacggtc
     6721 gaactgaccg tggcgctgca ctatgtctac aacaccccgt ttgaccaatt gatttgggat
     6781 gtggggcatc aggcttatcc gcataaaatt ttgaccggac gccgcgacaa aatcggcacc
     6841 atccgtcaga aaggcggtct gcatccgttc ccgtggcgcg gcgaaagcga atatgacgta
     6901 ttaagcgtcg ggcattcatc aacctccatc agtgccggaa ttggtattgc ggttactgcc
     6961 gagaaagaag gcaaaaatcg ccgcaccgtc tgtgtcattg gcgatggcgc gattaccgct
     7021 ggcatggcgt ttgaagcgat gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc
     7081 ctcaacgaca atgaaatgtc gatttccgaa aatgtcggcg cgctcaataa ccatctggca
     7141 cagctgcttt ccggtaagct ttactcttcg ctgcgcgaag gcgggaaaaa agttttctct
     7201 ggcgttccgc caattaaaga gctgctcaaa cgtaccgaag aacatattaa aggcatggta
     7261 gtgcctggca cgttgtttga agagctgggc tttaactaca tcggcccggt tgacggtcac
     7321 gatgtgctgg ggcttatcac cacgctgaag aacatgcgcg acctgaaagg cccgcagttc
     7381 ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga ccccatcact
     7441 ttccacgccg tgcctaaatt tgatccctcc agcggttgtt tgccgaaaag tagcggcggt
     7501 ttgccgagct attcaaaaat ctttggcgac tggttgtgcg aaacggcagc gaaagacaac
     7561 aagctgatgg cgattactcc ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt
     7621 aaattcccgg atcgttactt cgacgtggca atcgccgagc aacacgcggt gacctttgcc
     7681 gccggtctgg cgattggtgg gtacaaaccc attgtcgcga tttactccac tttcctgcaa
     7741 cgcgcctatg atcaggtgct gcatgacgtg gcgattcaaa agctcccggt cctgttcgcc
     7801 atcgaccgcg cgggcattgt tggtgctgac ggtcaaaccc atcagggcgc ttttgacctc
     7861 tcttacctgc gctgcatacc ggaaatggtc attatgaccc cgagcgatga aaacgaatgt
     7921 cgccagatgc tctataccgg ctatcactat aacgacggcc cgtccgcggt gcgctacccg
     7981 cgcggtaacg cggttggcgt ggaactgacg ccgctggaaa aactgccaat tggcaaaggc
     8041 attgtgaagc gtcgtggcga gaaactggcg atccttaact ttggtacgct gatgccagac
     8101 gcggcgaaag tcgctgaatc gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg
     8161 cttgatgaag cgttaattct ggaaatggcc gccagccatg aagcgctggt caccgtagaa
     8221 gaaaacgcca ttatgggcgg cgcaggcagc ggcgtgaacg aagtgctaat ggcccatcgt
     8281 aaaccagtac ccgtgctgaa cattggcctg cctgacttct ttattccaca aggaactcag
     8341 gaagaaatgc gcgccgaact cggcctcgat gccgccggta tggaagccaa aatcaaggcc
     8401 tggctggcat aatccctact ccactcctgc tatgcttaag aaattattca tggcaggagt
     8461 ggcagcatgc aatacaaccc cttaggaaaa accgaccttc gcgtttcccg actttgcctc
     8521 ggctgtatga cctttggcga gccagatcgt ggtaatcacg catggacact accggaagag
     8581 agtagccgtc ccatcattaa acgcgcgctg gaaggcggca taaatttctt tgataccgcc
     8641 aatagctatt ccgatggcag cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc
     8701 cgtcgtgaag acgtggtcgt tgcgacgaaa gtgttccatc gcgttggtga tttaccggaa
     8761 ggattatccc gtgcacaaat tttgcgctct atcaacgaca gcctgcgccg tctcggcatg
     8821 gattatgtcg atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg
     8881 ctggaagccc tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc
     8941 atgcacgctt cgcagtttgc tcaggccctg aagctacaaa aacagcacgg ctgggcgcag
     9001 tttatcagta tgcaggatca ctacaatctg atttatcgcg aagaagagcg cgagatgctg
     9061 cctctgtgtt atcaggaggg cgtggcggtg attccgtgga gcccgctggc gcgggggcga
     9121 ctgacgcgtc cgtggggaga aactaccgca cgactggtgt ctgatgaggt ggggaaaaat
     9181 ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt
     9241 gaagaactcg gtgcaacacg agcacaagtt gcgctggcct ggttattgag taaaccgggc
     9301 attgccgcac cgattatcgg tacatcgcgg gaagaacagc ttgatgagct gctgaacgcg
     9361 gtggatatca ctttgaagcc agaacagatt gccgaactgg aaacgccgta taaaccgcat
     9421 gcggtcgtag gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag
     9481 gcatcggtgc acaactacga cagaataccc agcggccagt gatgaccgat aaagtacagg
     9541 atgcctgcgg aaatcacccc ggcgacaata tcgtcgatca tgatccccat gccgccatgt
     9601 acattgcgat caaaccagcg gatcggccac ggcttccaca tatccagaat acggaaaatc
     9661 acaaacccgg cggcaaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac
     9721 ataccaataa attcgtccca gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc
     9781 gtttgatgac aaagatagac gccgatacag atccccaaca tcaccaccag cgagtagagc
     9841 tgccagggta aaaaggtcat cagataccag aacggaatcg ctgccagcga tcccatcgtg
     9901 ccaggaacga ttgggcttaa tccactcccg aatccgacag caagcagatg ccacggatta
     9961 ctcatcttca ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt catatccttt
    10021 ccagtcaaac gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt
    10081 catttgcccg atacaggtaa acggtacgcc aaggtgtccg agagcaacat ccagcgcgcc
    10141 acggttcagt tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag agagcgccca
    10201 gcgcagcgcc tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatgc
    10261 caggtcaata cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag
    10321 accgtcagag agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat
    10381 acgcggcgat ggacggagat gacgtttgat caagtagtca gcatctttag catcggcaac
    10441 ctgcaaacgg ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg taccggtcac
    10501 atagatccag tcacccggtt tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa
    10561 gccgtggata cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg
    10621 catatcgtaa taattgagaa gatcaaacaa actgtcgctg aaggactcaa gccacgcttc
    10681 gtctacgtcc ggtaaggtta atgccagcgt cagccaggcc ggatcggccc ccatcgctgc
    10741 cagatcgctt aggttcaccg ccagtgcttt ataagccaga tcagcaggat cgatatcagg
    10801 gaggaaatgg ttacccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg
    10861 gatattgaga agtgcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct
    10921 tacacggtca aaataacggg caatcaggga gaactcgcca catgccatac gttatgcctc
    10981 agcaggtaaa aaagaaaagg ccgacgatca tggaacggtc ttccgtgaat ctaccggcct
    11041 ggatatcact ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg
    11101 aacttatggc tgtcttctgc gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc
    11161 actttgtatg gcacatcgct acgtttagac agttcgtaca gcgcaatgcg cagtactgct
    11221 ttttctacct gtcccagttc ttccagcagg cgggacaggt atggcttcat cagtccgtcg
    11281 aggtatgcgg tattagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg
    11341 tcttttacat cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac
    11401 aactgccagg agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt
    11461 ttcacggatt tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc
    11521 aagcgcggtc agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat
    11581 cgcttgttca atgctttcag tggtcagaac cccaaaagca accggaattt cgctgtcctg
    11641 ggcaacatgc gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc
    11701 accacgaata accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc
    11761 acccgccgcc agcggcagct cataggcacc aggcacccaa acaacggtaa tgttttcatc
    11821 ttttacctga ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat
    11881 aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc
    11941 ttcaataatg ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt
    12001 tagcataata tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat
    12061 gcaggcaaac atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg
    12121 ctaatttctc aagccctggc agcgtgcata atccgcgggc gtcgctgcct aatagtttag
    12181 gtgcgatata gacaatcagc tcatcgacta aacccgcctg cagcaatgcg ccagcgagcg
    12241 ttggccccgc ttccacccag atgctgttaa tttgctgttt acccagttgc atcatcagta
    12301 caaccagatc cagatgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc
    12361 actcactaga atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc
    12421 gatgttccgg cgtcacgcga ttttggctat caatcacaat acgtactggc tggcggagat
    12481 tttgttgcgg gtagagcgcc tgagtttgtt catccagttc agaccaacgc actgttaagg
    12541 ctggatcatc cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgtgcgcgct
    12601 gtcgttgtac gtcgcgccgc gcctgaggcg aagtgatcca ctggctttcg ccgctcgcca
    12661 tcgccgtgcg gccatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc
    12721 gcatccgctt gagaaagcct ttattcaatt gctcggcttc actcatcatc agcccgtggc
    12781 tgacgtcaat gccagcctgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag
    12841 gatcctgcat cgctgcgacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg
    12901 gtggcgtacg gccatgatgg ctacagggtt cgagtgtcac atacgccgtt gcgcctttgg
    12961 ctttttcacc cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gctcgttggt
    13021 ggtaaccttc accgacaatt tcgccatctt tgacaatgac gcccccgaca ttcgggttgg
    13081 ggtgggtggt aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt
    13141 cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga
    13201 aactgcggta gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct
    13261 ccatcaccag attgccaatc atcttgctcg gtacttcgcg ctcaccggtg gcgcgcagct
    13321 gcgatttaat atgattgatt gccatttcaa cgtcatcgga actcaccgga cgtttttcca
    13381 gcgcccgcag cattccgcta cgcaatttct cttcattaaa cggttcacgc acgtcgttgc
    13441 tttttacaac acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac
    13501 acaccagaca ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa
    13561 ttaccttagt gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg
    13621 ttaacggaaa tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga
    13681 taaaggatcg atgctttcgc tgcttttacc ctctaccatt aggctaattg cgacgagaaa
    13741 gggacgacct atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct
    13801 tagcgcctgt gtgcagcaaa gtgaagtgcg acagatgaaa cacagcgtca gcacgctgaa
    13861 ccaggagatg acgcaactca atcaagaaac agtcaaaatc actcagcaaa acaggctgaa
    13921 tgcaaaatcc agcagtgggg tttaccttct gcctggagcg aaaacaccgg caagactgga
    13981 aagccagatc ggtactttac gtatgtcgct ggtgaatatt acgcctgata cagatggcac
    14041 cacccttaca ctacgtattc agggtgagtc gaatgatccc ttgcccgcat tcagcggaac
    14101 tgttgaatat gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcagaa
    14161 tcaattgatt aatgcccctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca
    14221 attaaagggg atttctgtgg atcagttagg ctttgtacgt atccacgaca ttcaacctgt
    14281 tatgcagtaa acgtatttcg ggacgatttt gtgcgtcccg caacatcttt ccccgtcatt
    14341 ttgttactct gcttacatca cctggattga tagtaaaagt ttgcaacaag ggcgaaagtc
    14401 agtacaatcc ccgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga
    14461 actgtgaaac gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac
    14521 gaaatattta gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg
    14581 gcatacatat gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt
    14641 ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga
    14701 gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc gcagatccgc aacgatacct
    14761 accttgagta cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg
    14821 cgccggtatt cttcggcggt aactccgatg ctaaaggtat ctggaaccac ggttctccgc
    14881 tgtttatgga aatcgaacca cgtttctcca tcgacaagct gaccaatact gaccttagct
    14941 tcggtccgtt caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata
    15001 aagatggtcg ccagagcacc tggtacatgg gtctgggtac cgacatcgat actggtctgc
    15061 cgatgagcct gtccatgaac gtctatgcga aataccagtg gcagaactat gacgcagcga
    15121 acgaaaacga gtgggacggt taccgtttca aaattaaata ctttgtgccg attaccgatc
    15181 tgtggggcgg tcagctgagc tacatcggct tcaccaactt cgactggggt tccgatttag
    15241 gggatgacag cggtaacgca atcaacggta ttaagacccg tactaataac tctatcgctt
    15301 ccagccatat tctggctctg aactacgatc actggcacta ctctgtcgta gctcgttact
    15361 ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa cttcggcaac ggcaacttca
    15421 acgttcgctc taccggctgg ggtggttacc tggtagtagg ttacaacttc tgattatgaa
    15481 aatgccggga tttattcccg gcatttctga ttgttaacaa cgtgtgcttt gttcatgccg
    15541 gatgcggcgt aaacgctttg tccggcccac aaaagcacgt aaattcaata tattgctgaa
    15601 ataacgtagg cctgataagc gtagcgcatc aggcaatttt acgttttaat cacttcttct
    15661 tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg acatctttct
    15721 gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggta tatttcgaat
    15781 gctcatgatc gtggcaatag agacaaagca attcccagtt actgccatct tccgggttat
    15841 tggtatggtc gtgatcaatg tggtgaacgg taagttcacg caggttggaa taaacaaact
    15901 cgcgggaaca gcgaccgcag acccacggat agatttttaa tgctttttcg cgatagccgc
    15961 tttctaaccg cgcatagttt tttgggatga tagccatgaa aagttacctg gatgaaatgt
    16021 agggttacgc gcagtttccc aagatgcggg gattttcgca atctccatac atcaaaaaaa
    16081 atcccgatct tctgaccggg attttcaaca tcaacgggaa cttgattacg gcagaattga
    16141 cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc
    16201 cagtttcaga gccaacgcag atgctacgta gatggaagat gcagtgccga tggaaacacc
    16261 gataagcatg gtcagcgaga agccttccag taccggacca ccgaagagat acagcatcag
    16321 gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga
    16381 cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaattcg
    16441 gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac
    16501 aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc
    16561 gtgcgccagc gcaataacca cccctgccgc cagtcgccac tcaaagcgga aacctacgta
    16621 cacgaggata gacagcagcg ctgccatcaa cgccatcgca ccggtttgcg caaggtctgc
    16681 ccccacgctc ggaccgacga actcaatacg cttcactgct gcattctgat tggtggattc
    16741 gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc cttcagcagg
    16801 cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcattg gctcttcaaa
    16861 accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat
    16921 ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca cgcccataat
    16981 aacgatagca gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat
    17041 aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc
    17101 gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg
    17161 cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg
    17221 cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta atcagcgtgg
    17281 tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca attgcctgtt
    17341 gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat
    17401 cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc agtgttgcgc
    17461 ctggcaacag cgacataata ccgacgatca agatcaagtt ggcaatcaga gcactggtcg
    17521 caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg
    17581 caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggtcggg ccaattgtac
    17641 gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgacagct
    17701 gacgggcttc gttcgggttg ttgatgccgg tgatacggaa gctgttaccc agacgagact
    17761 ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga ccatttgcat
    17821 ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt
    17881 ccttggtgaa gttagacata atgttgccac cagcgctatc gagcgagatg ttaacctgcg
    17941 gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg gtcagaatta
    18001 cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca gagtcgcccg
    18061 gtacgcgacc ggatgccgca gcggcctgat caacattggt gtttaccaga cggaattcca
    18121 gcgttgccgt cgcgcccaga atctctttcg cacgcgcagt gtcctgaata ccaggcagtt
    18181 caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt
    18241 ttacacggtt acgcaggata ttaatgttct gctgcaccgc atattcacgc gcttcactca
    18301 gacgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctgcta atcaccaggt
    18361 ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag
    18421 tgatgctcag gccgtagttg ttttctttac gaacagtggt atacgggatg cccttttcgc
    18481 gcaggtcact gcgtaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca
    18541 tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct
    18601 cagcatgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt
    18661 atttgtcacc cataacgccc attaatgctt cacgagcgcg caactgggtg tcagtggagt
    18721 cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt
    18781 cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac
    18841 cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga
    18901 tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa
    18961 ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc aggacggcag
    19021 ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata gcaatgtagc
    19081 cgttttccgc tactttggtt acgcgaccaa ccaggccacc gttcgtcaga acttcatcac
    19141 ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca
    19201 ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc
    19261 taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat
    19321 ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg
    19381 taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc
    19441 atcaaacgct ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg
    19501 ttgcaacggt caagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca
    19561 cactcaggat caagtgggcc agtatcgctc ttatacttcg cattgcggat tttcaccacg
    19621 ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac
    19681 atatcgatac cacgacgtac gccttcaacc aggtcttctg gtttaccaac gcccatcagg
    19741 taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg gtgcatatct
    19801 gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc gatatctacc
    19861 agacctttaa cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat gataccaaac
    19921 agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga
    19981 gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca
    20041 aagatcatga cgatatccga accaagatcg tactgaatct ccattgattt ttcaggatcg
    20101 aggaaaatcg gatcgccgtt gatcgggtta cggaagtgca cgccctgttc ggtgatttta
    20161 cgaatatcgc caaggctgaa gacctggaag ccgccggagt cggtgaggat cggccccttc
    20221 cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc
    20281 cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc
    20341 gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt ttccactacg
    20401 ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat
    20461 ttcatttttt ctccactacg tcagaaaaac agtccaacgt tttaaaccag cgccacggaa
    20521 ttactccccg acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc
    20581 gtaactaaaa aagcgatatt tctcttctac cgctgcttta taggcgttca tggtgtgttg
    20641 ataaccggca aaggccgaaa ccagcataat cagcgtcgat tccggcaggt ggaagtttgt
    20701 caccagcgca tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc
    20761 aaagaacggt tcgataagat cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac
    20821 tgaggtggtg ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc
    20881 taccacatcc tgcggcactt cagcgtattc cgagtgcatg atgtgatctt caatggtgtc
    20941 gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac
    21001 gcctttggcg cgtaattttt ccagcaaagg ttcgtcaaaa tgcagacctg cggtcggggc
    21061 tgcaaccgcg cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc
    21121 gtccggacgg tcgatatacg gcggcagcgg catatggccg atgctgttga gaatatccag
    21181 caccgagcgt tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt
    21241 tgcgttaata ctttcgtcat cgcccagcag cagttctgcg ccaggttttg gcgctttcga
    21301 ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat
    21361 cttgccgccg ctggctttac gcccaaacag gcgcgccggg atcacgcggg tattattaaa
    21421 aaccagaaga tcgccggggt tgagcttatc aagtaaatcg gtgaaagtac cgtgcgtcag
    21481 cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatgggata
    21541 gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac
    21601 tcagactaaa ataagaggcg ggtagtctag tgccggggcg ctctccctgc aacctttacc
    21661 cccttttgga tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg
    21721 cggaaagctc gctttccggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa
    21781 gttttccgcc cgacgtcgtg gctggcattc atatgcatcg acgtatcgac gtattaaccg
    21841 acaatctgcc ggaagtccgc gaagcacggg aatggtttcg tagtgaaact cgccgcgttg
    21901 cgcctattac gctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt
    21961 cgccggactt tccgctacag gaatttgtct gttatgcccg cgagcaagtg atgacgattt
    22021 tgccggactc accgccacgt tttatcaatc tgaacaatta cttgtggtca gagcagtggc
    22081 tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc
    22141 gcccacgtct ggatgccctg cgtgactcct ggtacgattt agacgctcat tatgacgccc
    22201 tcgaaacccg cttctggcag ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt
    22261 tataaaacgt tagttcatcc ataccgtagc cgaaatggca ggcaaagcca gaatcccgtc
    22321 agtcagttgc ccatgccttt ctttgcattg ccattgcacg gcattgagca acggtgacgc
    22381 gggtagcacg acttcacaag cttcgccacg gttgattgcc accagtacac gctgctgatt
    22441 cagcacgcgg acaaatacca ccacgttatc ttccgcatac agcacctgac agccgccatg
    22501 acgtagcgcc tgacttttct tacgcagcgc aatcattcgc tggtacagcg cgaataacgc
    22561 cgtatcctgc ttttccacct gccaggggaa cggtttacgg caaaacggat cgtttttgcc
    22621 atccagtcct acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca
    22681 gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt
    22741 atcgtggctg tcgagctgat taaacatacg taattgttgt tgatgagaaa gccctgcgcg
    22801 gtaattatcc atccaggcca tacaggtttg ggcatcaatt tgctgcggat cgtaagagat
    22861 atcggtattg gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt tcatggcggc
    22921 atcttccaca tcggcctgta accattgccg tgcatcgcca aaatgttcgc cgacaatata
    22981 cgcttccggc tgggtttctt tcgccgcttc ggtgctccca gcaacgtgct gcatattatt
    23041 gcgcgcccca cccgcctccc ctagcatatg caccacatcc agccgccagc cgtccatact
    23101 ccacggcgct ttcagccaat ggcggacaat actgtcttcc ccgcgataaa tttcattcac
    23161 cagactttcc gactgataat ccagcttcgg caagctggca tagccaagcc agtcgagcgc
    23221 cgtgccatca tcactaaacg agtaccagtc gcgccagggc gattcagggt tgtgacaagc
    23281 tccccccgtg ccacgattgt gcctatcaaa ccaggcatgg gaatcgccac tgtggttaaa
    23341 cacgccatcc agccccagcc gcattcccag ctgctgcgta ttgtggcgta agcgcagcaa
    23401 cgccccatca ccgccaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg
    23461 tacgctggga gctttaaaca ccggattgag atacagcgcc gtcacgccaa gctttttcag
    23521 atacggcagt ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc
    23581 ctgcgccgtg accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg
    23641 gtaatagaca tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa
    23701 gatctgataa aaaatctgat ccgcagccca ttgtgggccg atatccggta catcgacggc
    23761 aaactgctcc agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg
    23821 gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc
    23881 aatcgccgca cgccatgcgg tgacgccagg ctgctgctga ctgcgatgct tatgcatcga
    23941 tactgacatt tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc
    24001 gcccgtcagc cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac
    24061 cggcaggtgc catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc
    24121 catagcctaa tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc
    24181 ggataacttt cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag
    24241 gattactggt tttcagccag ctgacgatcg tggcgacgct taaacatcca gccaatcaac
    24301 agcacaacaa tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca
    24361 atcaacccga taataaagag caggaaaatc agcccgccga tggtcgttgc taccccaccc
    24421 ggcactttaa atttcagcgc cttaacttct tctggcggca aacagcgacg gaaggcaatt
    24481 tgcgacagca ggatcataat ccacacccac accgtggcga aggttgccag cgaagcgatc
    24541 accaggaaga cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta
    24601 gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc
    24661 ggcgcgctgc cctgctctgc cataccgtgg agcatacggc ctacgccaaa gacgtcactg
    24721 ttaattgccg atagcgaagc ggtcagcaca acaaagttaa gaatgctagc ggcaaaggta
    24781 atgcccatat gctggaacgt cagcacgaac gggctaccgg cagtgccaac ctgattccac
    24841 gggtagatag acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc
    24901 acggagttaa tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc ggcggtaatc
    24961 ccgataattt cgatcccacc gtaagcaaac atcaccattt gcaacgacat caccatccca
    25021 agccagccgt tactgaagaa gccgccgttg ctccacagat tatgaatacc ggtcggttgc
    25081 ccgccgttgc caatccccca gatgatgatg ccgaaaccag cgacaatcat gatgatgatg
    25141 gtggcgactt taaagaacga gaaccagaat tccagctccc cgaagacctt cacgctcatc
    25201 aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc
    25261 gggaaccaga cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg
    25321 atttcaaagc agtaggtcca gccggtaatg taacctgcca gcgggccgag gttttcctgc
    25381 gcataacgcg agaaagagct ggcggccggg ttatgtaccg acatttcccc cagcgcacgc
    25441 ataatgatat acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc
    25501 attttgatgg cgtctgccga accgtaaaac agcccggtac caattgctga acccagtgcc
    25561 ataaagcgta tgtggcgggt acttagccca cgctttagct tgttcttact ttccatcaat
    25621 tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa
    25681 tgttcagtga tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata
    25741 atggccagaa ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc
    25801 ggtaaacgct gcgcccagga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg
    25861 agaataccaa aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc
    25921 caccatgagc gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc
    25981 agcaccggta cggagatctg gatcagctgg cttaagccga ggttagaaac caccatcgag
    26041 aagccgccga ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat
    26101 tctgcacaag cacaggtcag gccaactgcc gttaccaagc aggcgatgaa gattaacgcc
    26161 gccagcagga agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca
    26221 gcgccgtttg cagactgatc gaccagcgac gcgctgtctg aacccagacg gaacagcgcc
    26281 aggtacagca gagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc
    26341 agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac
    26401 accattgcgc ccagcgtatc catggtcaga tagccgttaa cgaagccgtt agaaaacgca
    26461 gcgttttgat aagcctcagt cgccgtgctg atagaacccg ccggccagac aatcgcggca
    26521 acagacagga tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc
    26581 agcagcttgc ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag
    26641 ataaacagcg gcagcgcgga atcaccagtc agcggcgcaa tccccacttc aaaggaaacg
    26701 gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc
    26761 agcacgccag cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt
    26821 gccagcgcca ctaccgttaa taccggcagg ccaacggcag taatgaggaa gccgaatgcc
    26881 gcagtccaga cgtgttcgcc agcctgcaag ccgaccattg gagggaaaat aatgttacct
    26941 gcgccgacga acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat
    27001 tgatgggtca taaatcatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc
    27061 aatccctggg gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc
    27121 aggcgcggcg aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc
    27181 tgaagcgcaa tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg
    27241 tatatagcga taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg
    27301 ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt attttgactg
    27361 caaagaaaat aagccagcat cgactggctt atggcaataa aagatgacaa aggcggatta
    27421 atcgctgttt ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc
    27481 tactgtactc tcaatattca ggcgactttc gtgatgattc acagcatgtt tcacgatcgc
    27541 taaccctaat ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg
    27601 ctcggtcagg cgcggaatat gctccggtgc aatgcccggt ccgttatctt caacgctaaa
    27661 ttcggcaccg tgcggcactc gctgccagcg tacggtgata tgcgtgcctt ctggagtatg
    27721 attcacggcg ttatagacca ggttcgaaat cgcactgcgt agctgatctt cgttgccaga
    27781 cactttgagg ccgttatcta tctcaaaggt aaatgtctgt tttttctgac tcagagtctg
    27841 agcctcgcgc tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaattg
    27901 cgtcggcgcg gcttcaattt tcgacagcgt tagcaactgc ttcaccagcc cttccatccg
    27961 ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg
    28021 ctcatccatc atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct
    28081 cacgttggca aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg
    28141 cgccaccatc agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc
    28201 ggtgttgagc accagattga gcgggcgaga aaaatcacgc gttttcagat attgcgtaaa
    28261 ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc
    28321 aagaatttgt tgcgccagac cgttacacca gaaaataccg ctctcttccg tggtcagcac
    28381 caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc
    28441 acggcggcgt tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca
    28501 gctaccacgc cccggtggcg gggtcatact gcgatccacc cacagccacc atgaaaggcg
    28561 caataaattc cagaaatgcc agataagcag tcctgttacc gatgccagca aaaaccaggg
    28621 caggtaacca aaaaatgcac ccaggatgaa agccgggagg cagcaaagta gcagctccag
    28681 caccagcctt ttccacgaca gccgttccag cacgtaagat actcccgtta agaaatcata
    28741 agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt
    28801 accgcgcacg gtctgcacca tgcggtcatg cccgccgggc tccagtgctt tacgcaggcg
    28861 acgaatgtgg acatcgaccg tgcggtcttc cacatacacg ttagttcccc agacgtggtt
    28921 taacagctgc tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt
    28981 aaattctgtc ggccccatct ccagcggctc ttcgcccgcc atcactcggt gagatgtcgg
    29041 gtcgagactt aatccctgca tctcaatcac ctcttccacc gccattggcg aaatacggcg
    29101 cattaccgct ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg tgatatagtc
    29161 atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat
    29221 caccactgga atatcccggg tcatcgactc gcgcttaagg tgtttgatga actggatacc
    29281 ggagccgcca ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg
    29341 attcacagca cagtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac
    29401 gaagcagacc atttcacgaa ttggagcttc atcttctacg accagaatac gtctcgccat
    29461 gatttgccct gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga
    29521 tttatgacag atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc
    29581 gcagaaaaaa gcaaatggca catctgtttg ggtataatcg cgcccatgct ttttcgccag
    29641 ggaaccgtta tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt
    29701 aaaagccgcg aagctgaaca tcaggctttt cttgactggc tgctggagac aacacaaacc
    29761 catcaggtgg atgcgattat tgttgccggt gatgttttcg ataccggctc gccgcccagt
    29821 tacgcccgca cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg
    29881 gtggtactgg caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg
    29941 gcgttcctca atactaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt
    30001 cgcgacggga cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc gcgtgacatt
    30061 attaccagcc aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt
    30121 accgattatt accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg
    30181 cccatcatcg ccacgggaca tttaacgacc gtgggggcca gtaaaagtga cgctgtgcgt
    30241 gacatttata ttggcacgct ggacgcgttt ccggcacaaa actttccacc agccgactac
    30301 atcgcgctcg ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat
    30361 tgcggctccc ccattccact gagttttgat gaatgcggta agagtaaata tgtccatctg
    30421 gtgacatttt caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa
    30481 cccatggcag tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc
    30541 gatgtatcgc aggagccacc agtctggctg gatatcgaaa tcactactga tgagtatctg
    30601 catgatattc agcgcaaaat ccaggcatta accgaatcat tgcctgtcga agtattgctg
    30661 gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc
    30721 agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga actggatgaa
    30781 tcgcagcagc aacgtctgca gcatcttttc accacgacgt tgcataccct cgccggagaa
    30841 cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat
    30901 ggaagattga tttcacccgc gagccgttcg ccagcaacgg gctgtttgct attaccggcc
    30961 caacaggtgc ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcacgaaa
    31021 ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgccg
    31081 aatgtctggc ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca ttctggagcc
    31141 agaatcgggc gcgtaaccaa cccgacggta atttgcaggt gccacgcgta gagctggcgc
    31201 gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa ctgacagcga
    31261 cgttaaccgg gctggattac gggcgcttca ctcgttcgat gctgctttcg caggggcaat
    31321 ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg
    31381 gcactgaaat ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca
    31441 cagagctgga gaagctgcaa gcgcaggcca gcggcgtcac gttgctcacg ccggaacaag
    31501 tgcaatcgct gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttaattaccg
    31561 cgcagcagca agaacaacaa tcgctaaact ggttaacgcg tcaggacgaa ttgcagcaag
    31621 aagccagccg ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac
    31681 ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa tcttcgtcca cactgggaac
    31741 gcatcgcaga acacagcgcg gcgctggcgc atattcgcca gcagattgaa gaagtaaata
    31801 ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg gcgaagcagt
    31861 cagcagaatt acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct
    31921 tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg
    31981 atcgcgagca tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaatg
    32041 cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt tgctagcgcc ctggcgcaac
    32101 attccgagca acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag attgttcccc
    32161 aacaaaaacg tctggcgcag ttaatggtca ctatccagaa tgtcactctg gaacagacgc
    32221 aacgtaatgt cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg
    32281 ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gctcaacgtg
    32341 cacagttaca ggcgggtcaa ccttgtccac tttgtggttc caccagccac ccggcggtcg
    32401 aggcgtatca ggcgctggag cctggtgtta atcagtctcg attactggcg ctggaaaacg
    32461 aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat gcattaacga
    32521 agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag caagcactta
    32581 ctcaacaatg gcaagccgtc acgaccagcc tcaatatcac cttgcagcca caggacgata
    32641 ttcaaccgtg gctggatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac
    32701 gacatgaatt acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc
    32761 aaattgaaca acgccagcag cagctcttaa cggcattgac gggttatgca ctgacattgc
    32821 cacaggaaga tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc
    32881 agcaacgcca gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acgccgattc
    32941 tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata
    33001 actggcggca ggttcatgaa caatgtctcg cattacacag ccagcagcag acgttacagc
    33061 aacaggatgt tctggcggcg caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc
    33121 tacaggccag cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa
    33181 cactaacgca gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa
    33241 cgctggtcac acagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt
    33301 tggctctcac tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt
    33361 tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata
    33421 accgtcagca acaacaaacc ttactgcagc aaattgctca aatgacgcag caggttgagg
    33481 actggggata tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg
    33541 cccaggggct gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc
    33601 acgggcgcta tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata
    33661 cctggcaggc agatgcagta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg
    33721 ttagtctggc gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact
    33781 cgctgttcct tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg
    33841 atgcgctgga tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgttgaag
    33901 cgatgaaaga gcgtattcct gtgcagatca aagtgaagaa gatcaacggc ctgggctaca
    33961 gcaaactgga aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg
    34021 ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac
    34081 ggtgattggt agccgttctg gtggttgtga tggtggtatg aaaaaagtca ttttatcttt
    34141 ggctctgggc acgtttggtt tggggatggc cgaatttggc attatgggcg tgctcacgga
    34201 gctggcgcat aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc
    34261 actgggggtg gtggtcggtg cgccaatcat cgcactcttt tccagccgct actcactcaa
    34321 acatatcttg ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc
    34381 ttcgtcttac ctgatgcttg ccattggtcg cctggtttcc ggctttccgc atggcgcatt
    34441 ttttggcgtc ggcgcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc
    34501 cgtggcgggg atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac
    34561 gtatttaagt caggaattta gctggcgtta caccttttta ttgatcgctg tttttaatat
    34621 tgcggtgatg gcatcggtct atttttgggt gccagatatt cgcgacgagg cgaaaggaaa
    34681 tctgcgcgaa caatttcact ttttgcgcag cccggccccg tggttaattt tcgccgccac
    34741 gatgtttggc aacgcaggtg tctttgcctg gttcagctac gtaaagccat acatgatgtt
    34801 tatttccgtt ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat
    34861 ggtgctggga aatatgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc
    34921 agcagtgact gactttatca ttgtactggc actgctgatg ctctttttct gcggcggcat
    34981 gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg ggattatttg ccctttcagc
    35041 acctctgcaa atattgttac tgcaaaacgc caaaggcgga gagttattag gtgccgcagg
    35101 tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag gtatgatgct
    35161 gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc
    35221 gatgtcatcg ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt
    35281 gctggcgaaa ccactggggt agattactgt cacggtgcgc gttgcttatt gccggatgcg
    35341 gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt ttgtaggcct gataagacgc
    35401 ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg ttttttactc ttgtggccat
    35461 aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg caccggcgtt
    35521 tcacattcgc cgccgaagac aaattgttta atcaactgcc caaccgtttg atataaacgg
    35581 tctacattgc tcatcccgcc ccccaggaca atcacatccg gatcgagaat attcacgaca
    35641 tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc cgctaccgga
    35701 tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc gctcaaacga
    35761 cgataatccg tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg
    35821 caataacaag ggacttcctc gcgataacgc agctcgtctt catccatcca cggtagcgga
    35881 ttgtgtcccc actcgcctgc cgtgccattg ccgccgatat gcgcccgccc atttaatgcc
    35941 acgcccgcgc cgcatcccgt gccgataatc acggcaaata ccgtctgcgc tcccgctgcc
    36001 gcgccatcta ctgcttctga aaccgccaga cagttggcat catttgccag ccgcacttcc
    36061 cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac cgttgagcca ggttgaattg
    36121 gcattcttca ccacaccggt gtagggcgaa atcgaacctg gaatgcccat acctaccgtt
    36181 ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc
    36241 tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc ccctgcatcg
    36301 cccagtgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg cactgtactc
    36361 tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc aatgcaaggc
    36421 cgccgacaat tcgttatcat gcccgctaaa tttaacgaca aggccgtgga aattatcatg
    36481 ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa
    36541 gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca ggacatggcg
    36601 aagatgggct gggttcctcc gatgggatcg cacagcgatg cgttaacgca cgttgccaat
    36661 ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaaa
    36721 caggcgctgg aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa
    36781 accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgctgccgcg tgctttcagc
    36841 cgttttagcc agacaatgat gtggatcgac acggttaacg gtttgattat ggtggactgc
    36901 gccagtgcca aaaaagcgga agatacgctg gcattactgc gtaaaagcct ggggtcgtta
    36961 ccggttgtac cattgagcat ggaaaacccg attgaactga cgctgaccga atgggttcgc
    37021 tccggtagtg cggcacaggg cttccagctg cttgatgagg ccgagctgaa atcgttgctg
    37081 gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga gatcaccaat
    37141 cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca gcgcattcag
    37201 tttgtgatgt gcgacgatgg ttcgctcaag cgtcttaagt tctgcgacga gctgcgcgat
    37261 caaaacgaag atatcgaccg tgaagatttc gccctgcgtt ttgatgccga tttcatcctg
    37321 atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg tggcgaagca
    37381 caacgttaat tgctgatttt cctttaatgc cggatgcgac gcctgccgcg tcttatccgg
    37441 cgtacgaagc cacaccaggc atataattat tcgctacggc gagcaataat ttttagcgca
    37501 gcaatattat gcgttttacg ccgtaactga tctaatcgcc tcatccctat tcgcctgtca
    37561 gcagcacgca gaatattcac taacccccat ttatcgctgg taaattgttt tgttaacagc
    37621 tccgcttttg gggtatcaat atattcacgc aataaatcag aaattgaaca aatatccgtc
    37681 aggtaaggat accagcaagt ttcaccgtga aaattacgca ctccagcgtt acctgcaata
    37741 aagtctttcg ggtaatccca gatcacgttt ttatccaacg tgatccaata acgcggtaaa
    37801 tcggtacttc caccattttg gctgcgcatg agataacggg tacagtgaat ttgcaggttg
    37861 atcgttggcg tcagtaagtc atagatctcc cgctgtaact tgctccatgg atgttgtgtc
    37921 attgtttttc ctcaagccgg tgacgcgctt cactgcgaat tttccgcgtt gccccggttt
    37981 tcactaaatc acgtaactgc gaaagatcgg gttgttcgac aaataagagt aatgatagat
    38041 cctcttttaa ccacgccgtt ttcacatcgg cagccagctg tggattatta atagccagcg
    38101 accgatcctg agactcagtc aatgtcgtta acaacgacgc cggggtatgt gcgttgcgta
    38161 atgccgtttg gcgcaaatga tcattgcgac tttgcagcaa tgcacgccac cattcatcat
    38221 ctgatttatc ttttatcgta gccagttccg caaaaaatac ctggagatct tcactgatat
    38281 ttatatcaac cttgatgaat tatgaatata tcggaaattg aaaaaatcg
//
LOCUS       DAJTVE010000026        38318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000026 DAJTVE010000000
VERSION     DAJTVE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            179..1297
                     /gene="hyaA"
                     /locus_tag="OO439_001244"
     CDS             179..1297
                     /gene="hyaA"
                     /locus_tag="OO439_001244"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6315654.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1294..3087
                     /gene="hyaB"
                     /locus_tag="OO439_001245"
     CDS             1294..3087
                     /gene="hyaB"
                     /locus_tag="OO439_001245"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6315655.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3106..3812
                     /gene="hyaC"
                     /locus_tag="OO439_001246"
                     /pseudo
     CDS             3106..3812
                     /gene="hyaC"
                     /locus_tag="OO439_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            3809..4396
                     /locus_tag="OO439_001247"
     CDS             3809..4396
                     /locus_tag="OO439_001247"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6315656.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            4393..4731
                     /gene="hyaE"
                     /locus_tag="OO439_001248"
     CDS             4393..4731
                     /gene="hyaE"
                     /locus_tag="OO439_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6315657.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            4788..5459
                     /locus_tag="OO439_001249"
                     /pseudo
     CDS             4788..5459
                     /locus_tag="OO439_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
     gene            complement(5817..6428)
                     /locus_tag="OO439_001250"
     CDS             complement(5817..6428)
                     /locus_tag="OO439_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6315658.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLDWLATGKNEDECGKIGKNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            6585..6851
                     /locus_tag="OO439_001251"
     CDS             6585..6851
                     /locus_tag="OO439_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6315659.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            6862..8952
                     /locus_tag="OO439_001252"
     CDS             6862..8952
                     /locus_tag="OO439_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6315660.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQ
                     LMLERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            9035..9967
                     /locus_tag="OO439_001253"
     CDS             9035..9967
                     /locus_tag="OO439_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6315661.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLTGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9970..10191
                     /locus_tag="OO439_001254"
     CDS             9970..10191
                     /locus_tag="OO439_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315662.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKACGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            10204..10458
                     /locus_tag="OO439_001255"
     CDS             10204..10458
                     /locus_tag="OO439_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315663.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            10460..10741
                     /locus_tag="OO439_001256"
     CDS             10460..10741
                     /locus_tag="OO439_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6315664.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIDVVA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMVQFCREVRRRVSYPNRTRARG"
     gene            10738..11010
                     /locus_tag="OO439_001257"
     CDS             10738..11010
                     /locus_tag="OO439_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315665.1"
                     /translation="MSKVVRIIFEYKEDVIRKNADGTVHMGVSLDIRSTGIKQKNDSP
                     AMIFGVVMLAESRNFAELVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            11015..11308
                     /locus_tag="OO439_001258"
     CDS             11015..11308
                     /locus_tag="OO439_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315666.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            11320..11850
                     /locus_tag="OO439_001259"
     CDS             11320..11850
                     /locus_tag="OO439_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6315667.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRRFGDMQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11948..12490
                     /locus_tag="OO439_001260"
     CDS             11948..12490
                     /locus_tag="OO439_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315668.1"
                     /translation="MAYFYFKLDIVYTNKYLTEYQRDVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            12494..13027
                     /locus_tag="OO439_001261"
     CDS             12494..13027
                     /locus_tag="OO439_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315669.1"
                     /translation="MIDAKVLEGVKSWLRFSGRLTSRSLAEKMNMPLSSMVYFLRDAV
                     DAGVLTDRNGFYDIPRPRPVQAVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITGRVLRY
                     LIIDRTAASAGISAEVA"
     gene            13027..13542
                     /locus_tag="OO439_001262"
     CDS             13027..13542
                     /locus_tag="OO439_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315670.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            13546..14097
                     /locus_tag="OO439_001263"
     CDS             13546..14097
                     /locus_tag="OO439_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6315671.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            14094..14426
                     /locus_tag="OO439_001264"
     CDS             14094..14426
                     /locus_tag="OO439_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315672.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHLSALP
                     ELQGIHNG"
     gene            14564..14851
                     /locus_tag="OO439_001265"
     CDS             14564..14851
                     /locus_tag="OO439_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315673.1"
                     /translation="MEKEIKFAPKSIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFQR"
     gene            complement(14832..15071)
                     /locus_tag="OO439_001266"
     CDS             complement(14832..15071)
                     /locus_tag="OO439_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315674.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGLISREEAIHIINLAQKN
                     STPENTEDIQDGSYINTDWHFDNLRACMGITAEKD"
     gene            15142..15654
                     /locus_tag="OO439_001267"
     CDS             15142..15654
                     /locus_tag="OO439_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6315675.1"
                     /translation="MNRASLITLIHVAKRDLQLDHDTYTSALLAATGKSSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            15725..16147
                     /locus_tag="OO439_001268"
     CDS             15725..16147
                     /locus_tag="OO439_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6315676.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            16219..16719
                     /locus_tag="OO439_001269"
     CDS             16219..16719
                     /locus_tag="OO439_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6315677.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            16754..17182
                     /locus_tag="OO439_001270"
     CDS             16754..17182
                     /locus_tag="OO439_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315678.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRPCAAGADPR"
     gene            17166..17384
                     /locus_tag="OO439_001271"
     CDS             17166..17384
                     /locus_tag="OO439_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315679.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            17395..17622
                     /locus_tag="OO439_001272"
     CDS             17395..17622
                     /locus_tag="OO439_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6315680.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            17603..17914
                     /locus_tag="OO439_001273"
     CDS             17603..17914
                     /locus_tag="OO439_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6315681.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            17907..18197
                     /locus_tag="OO439_001274"
     CDS             17907..18197
                     /locus_tag="OO439_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6315682.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            18200..18781
                     /locus_tag="OO439_001275"
     CDS             18200..18781
                     /locus_tag="OO439_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6315683.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            18792..19040
                     /locus_tag="OO439_001276"
     CDS             18792..19040
                     /locus_tag="OO439_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315684.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            19040..20704
                     /locus_tag="OO439_001277"
     CDS             19040..20704
                     /locus_tag="OO439_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315685.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            20704..22293
                     /locus_tag="OO439_001278"
     CDS             20704..22293
                     /locus_tag="OO439_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6315686.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            22277..23608
                     /locus_tag="OO439_001279"
     CDS             22277..23608
                     /locus_tag="OO439_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6315687.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(23610..23689)
                     /locus_tag="OO439_001280"
     tRNA            complement(23610..23689)
                     /locus_tag="OO439_001280"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            23730..24203
                     /locus_tag="OO439_001281"
     CDS             23730..24203
                     /locus_tag="OO439_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6315688.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAESLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            24381..25505
                     /locus_tag="OO439_001282"
     CDS             24381..25505
                     /locus_tag="OO439_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6315689.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            25505..26452
                     /locus_tag="OO439_001283"
     CDS             25505..26452
                     /locus_tag="OO439_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6315690.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            26496..26849
                     /locus_tag="OO439_001284"
     CDS             26496..26849
                     /locus_tag="OO439_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315691.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            26846..27265
                     /locus_tag="OO439_001285"
     CDS             26846..27265
                     /locus_tag="OO439_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6315692.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            27262..27825
                     /locus_tag="OO439_001286"
     CDS             27262..27825
                     /locus_tag="OO439_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6315693.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            27829..28116
                     /locus_tag="OO439_001287"
     CDS             27829..28116
                     /locus_tag="OO439_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6315694.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            28116..29618
                     /locus_tag="OO439_001288"
     CDS             28116..29618
                     /locus_tag="OO439_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6315695.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGAGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            29627..29992
                     /locus_tag="OO439_001289"
     CDS             29627..29992
                     /locus_tag="OO439_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6315696.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            30007..30486
                     /locus_tag="OO439_001290"
     CDS             30007..30486
                     /locus_tag="OO439_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6315697.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            30614..32671
                     /locus_tag="OO439_001291"
     CDS             30614..32671
                     /locus_tag="OO439_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6315698.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            32658..34016
                     /locus_tag="OO439_001292"
     CDS             32658..34016
                     /locus_tag="OO439_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6315699.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPSVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLAGLPGDPSLSLTGKAGTPSGSLTTNRDSTTEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQNNIRLLRSVMQSAALVSQAQTVGKLLDQVIRQNTTHVQDTDR
                     AGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRRVEVISEQ
                     QD"
     gene            34000..35124
                     /locus_tag="OO439_001293"
     CDS             34000..35124
                     /locus_tag="OO439_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6315700.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDITGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            35114..35728
                     /locus_tag="OO439_001294"
     CDS             35114..35728
                     /locus_tag="OO439_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6315701.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     ALEGTGKSQGLFTMSDAVIAGIRYSGHVHHDNGEGSKTGVPENG"
     gene            35721..36158
                     /locus_tag="OO439_001295"
     CDS             35721..36158
                     /locus_tag="OO439_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6315702.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            36158..37240
                     /locus_tag="OO439_001296"
     CDS             36158..37240
                     /locus_tag="OO439_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6315703.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            37231..37791
                     /locus_tag="OO439_001297"
     CDS             37231..37791
                     /locus_tag="OO439_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6315704.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            37791..>38318
                     /locus_tag="OO439_001298"
     CDS             37791..>38318
                     /locus_tag="OO439_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6315705.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDN"
BASE COUNT         8949 a         9839 c        11201 g         8329 t
ORIGIN      
        1 aaagtttcac tacgctttat taacaatact ttctggcgac gtgcgccagt gcagaaggat
       61 gagctttcgt tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc
      121 ttgccgacca taagcgcccg gtgtcctgcc ggtgtcgcaa ggagaagaga cgtgcgatat
      181 gaataacgag gaaacatttt accaggccat gcggcgtcag ggcgttaccc ggcgcagctt
      241 tctcaaatat tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa
      301 gattgcctgg gcgctggaga acaaaccccg cattccggtg gtatggatcc acggtctgga
      361 atgcacctgc tgtaccgaat cttttatccg ctccgctcac ccactggcga aggacgtcat
      421 cctttccctg atttccctcg attatgacga tactttgatg gctgccgccg gaacccaggc
      481 ggaagaagtc ttcgaagaca tcatcacgca atacaatggc aaatatatcc tcgcagtaga
      541 aggtaatccg ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat
      601 tgagaaactc aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc
      661 gtcctggggc tgcgtgcagg ccgcgtggcc caatccgacg caggcaacgc ctatcgacaa
      721 agtcatcacc gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat
      781 gagcgccatc attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg
      841 ccgtccgctg atgttctatg gtcagcgaat ccacgataaa tgctatcgcc gcgcccactt
      901 cgacgccgga gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgcct
      961 gtacaaaatg ggctgcaaag ggcctaccac ctataacgcc tgttcctcca cacgctggaa
     1021 tgatggcgtt tctttcccaa tccagtctgg tcacggctgc ctgggctgtg cggaaaatgg
     1081 tttctgggat tgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca
     1141 ttccaccgcc gataccgtcg gtttaaccgc gcttggtgtg gtggcagcgg ctgttggtgt
     1201 gcacgcagtc gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac
     1261 cgaacatcag ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga
     1321 tacaccatca ataatgccgg acgccgcctg gtggtcgacc cgattacgcg catcgaaggc
     1381 cacatgcgct gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc
     1441 ggcaccatgt ttcgcgggct ggagatcatc ctacaagggc gcgacccgcg cgatgcgtgg
     1501 gcgttcgttg aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac
     1561 gccatcgaag atgctatcgg tattaaagtg ccggacaacg ccaatatcat ccgcaacatt
     1621 atgctggcaa cgctctggtg ccacgatcat ctggtgcact tctatcagct tgccgggatg
     1681 gactggatcg atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg
     1741 caaagtctct cctcttggcc gaaatcatcc cctggctatt tcttcgacgt acaaaaccgc
     1801 ctgaaaaaat ttgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac
     1861 ccgcagtaca aactgccgcc agaagctaac ctgatgggct ttgcccacta tctcgaagct
     1921 ctcgatttcc agcgtgaaat tgtcaaaatc cacgcggtct ttggcggtaa aaacccgcat
     1981 ccaaactgga ttgtcggcgg gatgccttgc gccattaaca ttgacgaaag cggcgcggtc
     2041 ggggcggtca atatggaacg cctgaacctg gtgcagtcga ttatcacccg cacggcggac
     2101 ttcattaaca acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa caaaccgtgg
     2161 agcgaaatcg gcacgggtct ttctgataaa tgtgtcctca gctacggtgc gttcccggat
     2221 attgccaacg actttggtga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc
     2281 gacttcaaca atgtgctgcc agtggatttg gttgatccgc agcaggtgca ggagtttgtc
     2341 gaccacgcct ggtatcgtta tcccaacgat caggtcgggc gtcatccatt cgatggcatt
     2401 accgacccgt ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg
     2461 aatgaacagg aacgctactc gtggatcaaa gcgccgcgct ggcgcggtaa cgcgatggaa
     2521 gtggggccgc tggcacgcac gttaatcgct tatcacaaag gcgatgctgc gaccgttgag
     2581 tcggtcgatc gcatgatgtc ggcgctgaac ctgccgcttt ccggtatcca gtcaacgttg
     2641 ggccgcattt tgtgccgcgc gcacgaagca cagtgggccg caggtaagtt gcaatatttc
     2701 ttcaacaagc tgatgactaa cctgaaaaac ggcaatcttg ccaccgcctc cacggaaaaa
     2761 tgggaacctg caacctggcc gacagagtgc cgtggcgtcg gctttaccga agcgccgcgc
     2821 ggggcgttag gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg
     2881 gtgccgacca cctggaacac cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa
     2941 gcggcgctga tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact
     3001 ctgcacagct ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc
     3061 gagctgatct ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc
     3121 gacaacgttg tcagccacta tgtctttgaa gcgccagtcc gcatctggca ctggctgacg
     3181 gtgttatgca tggcggtgtt gatggtcacc ggatacttta tcggcaagcc gctaccttcc
     3241 gtcagcggcg aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc
     3301 gccgggatgg tttttaccgt ggtcttgctg atgcggatct actgggcttt tgttggcaat
     3361 cgatactccc gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg
     3421 tggtatgaaa tccgctggta tctgttctgg caaaacgtcc gagtgccgat ataggccata
     3481 accccatcgc ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca
     3541 ccggctttgc gctgtacagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg
     3601 tggtggaatt tttctactgg acgggaggca actcaatgga cattcacagc tggcatcggc
     3661 tggggatgtg gctgattggc gcgtttgtga tcggtcatgt ctatatggcg ctgcgtgaag
     3721 acatcatgtc cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat
     3781 ttggcaaaat aagtaacaag gagcgttcat gagcgagcaa cgcgttgtgg taatggggct
     3841 gggcaacctg ctgtgggccg atgaaggttt cggcgtgcgg gtggcggaac ggttgtatgc
     3901 ccattaccac tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt
     3961 gctggggtat gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct
     4021 ggaacctgga acgctgcgaa cctatgccgg agaacgtatt ccggcttatc tcagcgcgaa
     4081 gaaaatgagc ctgcaccaga acagtttctc cgaagtgctg gcgctggcag atatccgcgg
     4141 acatctgcca gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg
     4201 cggcagcctg agcgaactgg cacgggagca actgcccgca gcggaacagg cggcgctggc
     4261 gcagctggct gcatggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta
     4321 tgactgtctg tcgatggaaa attacgaagg cgttcgcttg cgccagtaca ggatgacact
     4381 ggaggagcag ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg
     4441 cgcggctgga cgccggttag tgaattccgt cttgacgact ggcttacgca agcgccagac
     4501 ggcgtggtgt tattaagcag tgacccgaaa cgcacgccag aggtcagcga taatccggta
     4561 atgattggcg aattactgcg cgagtttccc ggctacacat ggcaggtggc gattgctgac
     4621 cttgaacaga gcgaagccat cggagatcgc tttggcgtct ttcgctttcc cgccacatta
     4681 gtgtttaccg gcggaaacta tcgcggtgtg ctgaatggta ttcacccgtg agcggaactg
     4741 ataaacctga tgcgtgggct tgtcgaacca caacaggagc gtgcctcatg agcgaaactt
     4801 ttttccatct gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac
     4861 tgccgatcag ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc
     4921 acagcccgca ggtgattgcg ctgttaaacg agttacaaca tcaactaagt caacgccagc
     4981 cgccgttggg cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact
     5041 ttatcaatac gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca
     5101 gcgaaagtga aatacaggag gcgatcttct gcggattatg gcgggtgcgc aaacgtcgcg
     5161 gcgaaaagtt gctggaggac aaactggagg ccggctgcgc gccgctggcg ttgtggcagg
     5221 cggccacgca aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga
     5281 tgaatggcct accgttggcg catgagttac tggcgcatgt acgtaacccc gacgcgcagc
     5341 cgcacagcat taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac
     5401 gtctctgtgg gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca
     5461 tgtaaccagg tgaatcattt gataccgaca agggttttag tgaaaggagg tgaaatcacc
     5521 tgtaagatgc tgaaataaaa caggtgatgt atacttcgga cggaaaactc tttttgatac
     5581 cgactgctca acaaatagtc aaaagatcaa aaaacggcga cacatttgat actgctaatt
     5641 tacccttaaa atcttcttca aaacatcttc aaactgccaa aatgctcaca taatcgatct
     5701 aactataatc agtatgataa tcatcatgaa ttactattca atagcgtgat tatcttggga
     5761 tgagtcatat gatctctata tgtatttaca gcaccacctc atgatagcga tggcgcttaa
     5821 ccaacaacat ttttctttac tgtcactgcc cctgattcct gggtggcgtt attgctcgag
     5881 ttgtacttct caataacgcg caaaatctct ttgtctgttt cttcacttcc cgccattcct
     5941 atctttattg cattttttag tacgggacgt aatggcaagg catcaatgat gctttctacc
     6001 gcatcctggc tgattgtaga tgtattcatt gctgccagcc tgacaaggtt gttagtacct
     6061 tctcgcctga tgaatgtaag gattaattgc ttctcttggg ggcttagaga gctaagcgcc
     6121 tgatcaatac ccgaacctga atcgcaattt tttccgattt ttccgcattc atcttcgttc
     6181 ttgcctgtgg ccaaccagtc gagtgaacaa ttacaggctt tagctattaa ggccagttta
     6241 tccaaaggag ggaagttttt ccccttcctg taatttctta ctgtggcttc agatagtcca
     6301 caaagggtcg ccagttcgac gtttgtcatg tttcccatag ccatagctaa gcgttctgaa
     6361 aatcggttta ttccttccat atcaataaac cgttccgcac tggatacggt tttttctgcc
     6421 ttatccatat aaccccctat aaaacaaaga aattatcgac atcgcattta atttcgatat
     6481 gctcgcaaaa aaccgttgat atcgaattat tttgcgatta tgatttacct aaatcgataa
     6541 ccggttttga ttatcggttc aggtaaacat gagaggatca cacaatgagg gtatccagac
     6601 aagcatcgaa taatttgttt gttcgagagg ggtgtgactg gtctgctaaa gcggtatatg
     6661 cagcccttga aaacaaaggc acagacctcc ctgaactgga aagggagtta ggattaaaaa
     6721 gcggatcaat gcgcaatgtg ttttaccgcc agtgccgtaa atacgaagcc gccatcgccg
     6781 aaaaaatagg ggttgaacct gctgtaatct ggcccagcag ataccaggtg gacgccagag
     6841 aagtggcttg atgggggatg tatgtgggta acagcaaaag aatgtgttgg tcttcctgat
     6901 ttaccaatga tggaacataa cattcgccat cgactcacaa aagcagctgc gggaaatgca
     6961 gccatgttac gcaaacgtaa ggggacaaaa gcaatcgaat ttaatatcga ttgtttgcct
     7021 tctggcgcgc aacaagtatt gcgtgaacgt tatgcccttc aactgatgac gcaaaaagct
     7081 gatgaatcac cggctccggt ggtgacaaag gccagacgct catctgccgt ggttgatgcg
     7141 gtggaggcat atcgcggatc accacaactg atgctcgaac gcctcaatgc cctgactgaa
     7201 aaccagcgcc aggtggctga tgcacgaatc gcgatcgtca gcgaagttct gaaagtcgcg
     7261 caacaacctg gtttcagctg tgcgaaggct atccggttta tcgttgacaa cctggcacgt
     7321 tcgcaactgg acgagcgcat tgtggcaatg gttgagacgg cgaacgccaa aaagggaaac
     7381 agccgtgcgt tgagtgaaat cacgctgaaa cgctggattg cggcctttaa caaggcacag
     7441 aacgccgctg aacgcctgct tttactggca ccgggtaaac gccaggaaat aaaagccgaa
     7501 gatattaact ggctgcccga atttctggcg cagtatcgcc agtcaaacgg ccgaccaatg
     7561 accgaggctt acgaggattt tgttgctgaa tggcagcacc ggcacgctga tgagccttat
     7621 atgctcgata tcatgccctc ttatgacacc attcgccgcg caatgaagaa actgccggaa
     7681 gtggtgaaac aaaaaggccg ggtaactggc agtgaatacc gtcagcttga gggattcacg
     7741 cgccgcgact ggtccagaat gccggtgaat tatgtctgga ttggtgacgg tcacggcatg
     7801 aagctgaaat gcgcgcaccc ggttcacggg cggccattcg caccggaagt gacctttgtt
     7861 atcgacggtg gcacgcgctt tgtggtgggc tggagccttg acctggctga aaatgttttc
     7921 gccgtagccg gtgccataca gcacggtatt cgccatcatg gcaaaccgtt tctgtattac
     7981 tcggataatg gctccgggga aaccgccgac gtcctggata aggagattgt ggggatactg
     8041 ccacgactgg ggattaatca cccgacaggg attgccggta atccgcaggg acggggcatt
     8101 atcgaacggc ttaaccgcac attaccgatg cgcatagccc gtaaataccg cacctatttc
     8161 gggaaaggag cagatcgcga gacgttacgt aaaaccaacc gcgatttacg ctcggcattc
     8221 agtgccctgc aacagggcaa acggctgaac gcccggcagc agtcagcgat gcgtgattta
     8281 ccgtcctggt ctgaactgat tgatgccatt cgtgacggtg ttgagtggta caacaaccgg
     8341 ccgcacgatg aattacccat gaagccgaac ggtaaacatt acagcccggc ggagttcaga
     8401 aaaaaacgcc tggcggaaga ggacacggaa attgaatggc tgtccgatgt tgaactgcgg
     8461 gatatgttcc ggccgatggt ggagcgccct gtaagacgct gtgaaatacg ctggctgaat
     8521 aatatttact acgcgcccga gctgcgtgac gagcatggcc gtaaggtact tatcagctat
     8581 gacattcatg atgccgaacg aattaccgtg cgccgcccgg atggcagcgt gatttgcgag
     8641 gcggtatggg acggcaataa acgcgaagcc ttccctgtca gcgcggaata ctacaaacag
     8701 cagcagcgcc ttaaaggcat gcgtaaacgc gcagaggaaa aaatccgtga tgccgaggat
     8761 gaggttgtca acgtgctgga gcacaagccg caggagccct ggctggaaaa catataccgc
     8821 cctgtgggta atacggtgac cgttcagcaa ccggtcgctg acgatgaacc tgatgaagaa
     8881 tacgagcgta atttccagcg gggattgcaa ctgctcgaag cgaaattaaa agaaagtgat
     8941 ccgctggcct gaaataaaaa aataacccga ggggtgactc aggttatttg aggtttcagt
     9001 ctcggagaat gctgtctcaa gcgaggtgaa taatatgacc gatattaacg atgtaatcaa
     9061 gaccattgat gaacttattg atggcggcgt actgacgcag tatgccatcg ccagagaggc
     9121 gggaatttcc gacggcacat tatcggcttt ccgtaaggga aaatataaag gcgataacgc
     9181 tgctgtggct gcttccctgc gctcctggta tgagaactgg aataaacaaa gcgcactgcc
     9241 ggaaccgccg cagtttgtgg aaactcagac agtccaggag ctgcgcgcac tgtttcaggc
     9301 ggttcgcctg atgggctgta ttaacgttat tgtgggcgta ccgggtgtgg gtaaaacggc
     9361 cactgcccgt aattactgcc aggagcaacc aaacacctgg atgatcaccc tgtcacccgc
     9421 gcactccagc gtcacggagt gtctgctgga gctggccgat gcgctgggga ttgattacac
     9481 ccgcgcgaac aaaggggcat tatcccgcgc catccgccgt cgcctgacgg gaacgcgtgg
     9541 actggtgatt gtggatgagg cggatcatct tggtattgac ggtctggagc aactccgggc
     9601 aattcaggac gccacgggga tcgggatggt gcttattggt aacccgcgcg gattgtttaa
     9661 aggtggacgc cgcgcctttg atgatttatc gcgcctgttc agccgtcttg cccgtacaaa
     9721 acaacttcgc aaggccaaaa aggcggatgt gctggccatt gccagggcat ggggtatcag
     9781 tggtgaggcc gagctggccg tcatgcaggc tatcgctgaa aagccgggag cattacgtgt
     9841 tctgacacat acgcttaacc aggcgtggct caccgccagc ggtgaaggcg cggcgctgac
     9901 agaaaaacat attaatgcgg cctttaaaga ggtttatacc aaccctgaat tactctcaca
     9961 ggtgtgatta tggcggtatt taatattcct gatatttacg gacgctttta cctggttaat
    10021 ttcgataacg tgaaggtgat ttcactggcc gaaaataaag catgtggcga tttacttttt
    10081 gaatttaatg accgcacacg aatggtgata tctgccggac ttgatcgcga aggtgcgaca
    10141 gaagtttaca gcggaatatg ccgttctgtt ggtgcgaaac aagtcagcta aatgaggtgt
    10201 tatatgaata tgcaatcctg cggtaacaaa atgaatttat tcgactccct gaacagcgcg
    10261 cgccgtctga ccgaacttgc cggtgcggta ctggaacgca gtaagcgcta tccgcaacgt
    10321 tttgcactga aaaccacgcc gccggtaggc aacgtgcagg gaaccggtga aattgaaatc
    10381 accatacaga ccaacggcct gcgccgccgt gtgaaggcca cccgcatcag cggctgcacg
    10441 gtttactggg aggtgtgagg tgaaaaaaaa tctcattgca tgggcgtggt cgagtggtct
    10501 tattgagttt ggttacgtcc tgccggaagg tgcattgccg atagttgccg gaaagcccgc
    10561 cacggtacgg catgtgattg atgttgtggc gcgtcatgga cgcgatgaac aggaacagct
    10621 actggttcct ggaataccgg aagcggtgac ggaagaagaa gcctttaatg ccatggttca
    10681 gttctgccgt gaggtcagac gccgggtcag ttatccaaac agaacgaggg ccagaggatg
    10741 agtaaagtcg tgcgcattat tttcgaatac aaggaggacg ttatccgtaa aaacgctgat
    10801 ggaacagtgc acatgggggt aagtctggat atacgttcaa ccggaataaa gcagaaaaat
    10861 gatagccccg ccatgatttt tggggtggtt atgctggcag aaagcagaaa cttcgcagag
    10921 cttgtggcaa tgaaagccag tgcattcatg aaagacaggg gcatggcttc cggggttatt
    10981 aacggtaatg aatttaatca gcaggggtaa ttccatgagc aaagtacgcg ttatttttga
    11041 atttgagcat gtttcgcatg acgaaaaacc ggcaggcaat gacagtgttg aagtgcatga
    11101 aaagattggt gtggatgtaa aaacagaacg tgatacggat aacaggccga cgtcactctg
    11161 tgacgtttat gcaagtattc tccagtatca cagtcctgaa attattcagt ttctctcagc
    11221 ggaatttcag gcatctgtac aggcttttgg ggcggatgcc atcattaaac gccaccgcgt
    11281 gcataaagca tcaggcacac tgcaataagg aaaaacaaaa tggctaaacg cgttacaaaa
    11341 ttaaaggccg cagccgaggc agcaccgcag acccgtgaag aggtcagccg cgatatccga
    11401 cgctttgggg atatgcagcg tgaggcacta cgcctggaaa cggcgatgaa tgacgaagtg
    11461 gcagaaatca ccgcccgtta tacgccgcag attgaaaacc ttaaaaaaga aatcaaagtg
    11521 ctttttaagg ggattcatga ctggtgtaaa accaaccgca atgagctgac gaacggcggc
    11581 gaaaccaaaa cagccaatct gaccaccgga acggtgtcat ggcggctggg aacgccatca
    11641 tgcagcgtca gccgtgatgt ggaaggcgtg attgaaatgc tgcgccgtat gggccttgag
    11701 cgcttcatcc gcacgaaaga ggaagtgaat aagcaagctg tcctggcgga gccagatgcg
    11761 gtgaagggca ttgccggtat aaaggtgaat aaaggcgctg aaagttttta tgtcgagcct
    11821 tttgaacagg acgccggact gaataaataa caccgcatta aatctttaaa tatcacatcg
    11881 tgttaattat ggcgctcgcg tcaggggact gcctgcgcct gtaaactgaa aataaggaac
    11941 aggaaatatg gcatattttt atttcaaatt agacatagtg tacacaaata aatatttaac
    12001 cgaatatcag cgggatgttt taccgctacg caacagtatt cttcgggcat tactgaaaaa
    12061 tacaggcgct gccggattgc gcttaaagcc gttcgccatg gacgtaatca gtgagtttta
    12121 tttttctggt tctctgcctg cgggctggcg taagcgcgat gatgtggctt ttatcgggga
    12181 cggaccgtgc tttattgcca gacctgatga gtcatgccct gaaggtccgg cgattgccgc
    12241 aatgattgaa accgctgaac gtgagttaag aaagcgtcct gatttccttg tctggctctg
    12301 tgaaaagctg ggggtaatga gaatcccctc catgtttaac acggactcct ggtggacccc
    12361 gtcgctctcc cgtgatgccc tgtgcgtggt gtttaaagta ggcgcttatg gcagggaaat
    12421 aaaagggggt attcctgaag aatgccagga aattaaacat tctgaatatg tggcgctgac
    12481 ggaggaataa ttcatgattg atgcaaaagt gcttgaaggg gttaaaagct ggctgcgttt
    12541 ttctggccgt ctgaccagtc gttctctggc agaaaaaatg aatatgccgc tatcctccat
    12601 ggtttatttt ctgcgtgatg cggtcgatgc cggggtgctg acggaccgta acggttttta
    12661 tgatattccg cgtccccgcc cggtgcaggc ggttcgtcgc aaatgcagcc aggaaggtgc
    12721 ggctgatgat gttcagtggt gcagcttcag aaaatccctg ccgtggattg aggggcatga
    12781 tattccgtcg atggcgtggg aatttgctca gggagtactg acctgcgaaa ccgtttatgt
    12841 ggtggctgaa gttgatgagc aggccatgaa agaaggcgtg ccccagtttg tgatggcgta
    12901 tatcgacatt cgcctgggcg tcattatctg cggcttaagc ggctggaaca tcaccgggcg
    12961 cgttctgcgt tacctgatta ttgaccggac agcggcatcg gcagggatat ctgcggaggt
    13021 ggcgtaatgt tctttaaaac atcaaaccct tccgcgctgg ctgcgtggca aaaataccag
    13081 caggactgcc agaaagttaa ggatgaggca aaacgccttg aggccgtgct gaatgttgcg
    13141 tgccggtcgg tatttgtatc cggtatcagt ggattttgct ttaagggact gcgttttacg
    13201 gaggacaaat atccttttca tcgcgactta tggcgaaaac cgactgcgtc gaatggctgg
    13261 agctgcacac cgcgcacatc acgtatccct aaaaccctgc gtgttgcctc tgatgaactg
    13321 aacagtctgt ggcgtgaata ttcgcccgtc acgtatgcca gaaccgatgc gctgttgttc
    13381 tggctgggta ttgatttctc ggcaatcctg tttggccctg tgaagtggtt ctgcgttgac
    13441 gatgtgattt accttcagtg cggcgtaaaa cccgcaaaac agaaaatgac cgaaatactg
    13501 tctgatgagt tttatgctgc tgaaaagcga gtcagggggt gatgcatgat gaaattacaa
    13561 cccatgggac taaaaggtcg cgcccccgct catgtccgtc cgtggacacc tgaagaagat
    13621 gcgctgctga ttgcgcttta ttcatccacc ccggttaagg atattgctgc cagaataaaa
    13681 agaactgtct gggctgtata taaccggact ggtgtattgc gcagttcata cccggagtta
    13741 ctgaaatata aacacccaag atttacacct gatgaagata agtttattcg aaaaaatgcc
    13801 agaacaatga cctgccagca aatgggagaa tatctcggac gtaataaaga ttctgtcaga
    13861 tgtcgggcag gaatgattgg tgctggatta acaaagtgcg gagagttacg ccccggcacg
    13921 cgcatatctg atgatgatgt acgtcttata cgtgcgctgc gtgattccga ttacccacgc
    13981 cgtctgtcat tccgggaaat tggcgaaaag tttggaatat ctgaacattc tgctcacgca
    14041 gtttattacc gtcgccggac tgccgaggac gctgtattac gggagttaac gccatgataa
    14101 cgaccttatt tgttgaatca gatgaacctc ttgtgtgtgc cgccggaatg ccgctctgtg
    14161 gcggaacgct gaccggcgtt tatttcgggg atttgcgcgg ttatccctgg cattcactgg
    14221 atgacgcatt tccgcctgat atggaggctg tcgtgctgat tgttcagtat ggtgaccaac
    14281 aggaactgcg catcggccat atggggtatg aaggcttttt tattgatgaa gaaaccggag
    14341 cctgccttga agatgaggac ggacaggtga cgcactggtg ccatctttcg gccttgccgg
    14401 aattacaggg gatacataat ggatgagcaa attgttgaat gtcccacctg tggaaatgag
    14461 gacccggagt atctgaaaga gtgcccacat tgtggtgaga taaaatgtaa ccactgcgat
    14521 atgggcgacg acactgcatg tataaattgt gaggatgaat aatatggaaa aagaaatcaa
    14581 atttgcgcct aaatctatcg acgaagaact ggctaaaatc ggcatgcttg aacgtatgcg
    14641 ggacattatc gaatatgcga taaaagaaaa tcttgcagcc agagaagccc ttttgataat
    14701 ggagcgggag attaacctga ttaaggatgc tgtatctctg gataataaaa tagcccgcga
    14761 ggaatacgtt cgccgcaggc tcggtgttga tggttcagca attcttacat ctgaacatta
    14821 tgcaaaaatt tttaatcttt ttcagcggta atccccatac aggcccggag attgtcgaaa
    14881 tgccagtcgg tattaatata tgagccatcc tggatatctt ctgtattttc cggggtagag
    14941 tttttttgag cgagattgat aatatgtatt gcctcctcac gggaaatcag ccccttatcg
    15001 aacagggtta ccataagatt tccagccatt gtgaatgcgg caacatcaag tgcatttttt
    15061 atatctgaca tagatttttc cttttatgtt aattgacatg gcggtgcggt aacaccgccc
    15121 attttttatg gagccacgat aatgaatcgc gcttccctga ttaccttaat acatgtcgca
    15181 aaacgcgatc tacagcttga ccatgatacc tatacatccg cgctgctggc agccaccggc
    15241 aaatccagtt gccgtgatat gtcacccggt gagttatccc gcgtgctgga tgttttcaaa
    15301 aaacgcggtt ttaaagtgcg tcagaagccg gttaaccggg ccttaaaacc gggtacggtg
    15361 accgccaaaa ttcgcgccat ctggaaagtg atgcaccggc agggatttat ctctgatggc
    15421 ggggaaacgg cccttaaccg ctgggtgaaa tcgcagacgg ccgcgcagaa cgacggcgaa
    15481 ggtgtggcta actggcagtg gctggagcaa caacccgcgc tggcctctga tgtgctggag
    15541 cgtctcaaac gctggcaccg ccgcaaaatg ctggcagccc tggatatgtc ggaacgtgca
    15601 gtgatgagtt atgagcgtgt ttgcaggctg tatgaaaaat cattcccccg ttaaccccaa
    15661 atcccgccac agcgcgggat ttttatttta aacttgccct gtgtaatgat gaccggaggc
    15721 gggtatggct gaaacacaga tgagcatgtt tggtgacagc gaacagctgc acgcgctgat
    15781 tgaccgtctg gatgatattc ctgatgatga gctgaaaaag aactggcccg gcacgctgcg
    15841 ggacctggtt gatgtgatca gtgctgaact gcaccgtcag gggattgaac ctgctcaggc
    15901 gggaatgctt gcccgtaagg ttgcagccgc acaggccggg tacatgggcg ggcgtggtta
    15961 ttatctcccg gtcggtgaat cccttttcac cgaactgcgc aataatgaaa ttttttcccg
    16021 ctggagccag ggcgaaaaaa ttgaaaccct gcgccgccac taccagatgt cagagacgca
    16081 gatttactcc gttatccgca accagcgtcg cctgcatcag gagcgcacgc agccccggct
    16141 tttctgatat tccgcaaatc ctgccccctt ttcctcacgg ttacgctgac tcagagaaca
    16201 ccatgagaca gcgtaacaat gccaaaactc cccgcacccc tgcgtaaaaa gctgattgcc
    16261 cttgttctgg ccggagccgg gacgttcacc attgccacgc actacaccgg ctactgggaa
    16321 gggaaagaaa acaccacgta cattgatccc accggcaccc ccaccatctg ttacggccat
    16381 accggcccgg atgtgaaacc ggggatgaca aaaactgatg aagagtgtct ggagctgctg
    16441 gaaaaggaca tgaaatgggc ctttgcggct atcgatcgtc atgttcaggt gccgcttacc
    16501 cgtggtcaga cggtggcgct ggcctcctgg attttctggg ccggtgaaac gaactttcgc
    16561 aactccacgc tcctgcgcct tatcaatgcc gggcagatgc ccgcgtcctg taagcagtat
    16621 atccgctgga tttattcaaa gggggtaaaa ctccccggtc ttgaggcccg ccgttcggcg
    16681 gatgaatggt tatgtcgtta cgatttgccg aaagtctgaa ccgcttctgg cgaccgctca
    16741 tgattgcgct gctgtgtgcc gttgtgctgc tgcggggtgt gctgtgtctg cggtgaacaa
    16801 tcccttaccg gccctgattg ccggaggctg tatggcggcg ctgggggtga tgagcgtgct
    16861 ggctgccgtg gtatggggaa tgcaccagaa gacgcagcgc cttgaggaca ataatcatgt
    16921 gcttgtgcgt gaacgggacg aagcccgtct ggtgctggca aaccagcaac gcaccctgca
    16981 actcatttca caaatcagca aggcggccac gaatgaaaaa cagcagaaca tgcagcacag
    17041 cgaggaacag cagagcattg tccgccggtc actggcaacg gtgcctgctg ccgctgtgcc
    17101 tgtgcctgat gatgtggctg accgggtgcg gcgggccgtc tgtgaaattc gtccctgtgc
    17161 cgccggtgcc gatccccgct gaatggctgg ctgactgcct ggtccctccc gcgccgcagc
    17221 cgttcacgtt tggtgcatcg gtcacttaca acctgcaact gctggcggtg attaaaaact
    17281 gcaacgtgga caaggccagc atccgccgtc tggagacaca gcgacagcat gaatttactg
    17341 atatggccgg aacgactgct gttccggcag gaaagacgaa gtaaggaaaa aggtatggat
    17401 gattcagatt gtgctcaggc agtcatggag cgtgcaacag aacgcgccct ttgtgaccgg
    17461 ctgacacgta agcgccgggt ggcggagaca ccgggcaggc gtgtatgcgc ggactgcggg
    17521 ggtgacattc cggctgcccg tctggttgcc gtgccggatg ccatccgttg tgtgaactgc
    17581 cagaacatca tggaggccgg tcatgtgggt cagcatcgtt aaagattatg tcgtgccgat
    17641 cctgtcggca acggccacag ccggggggat cttcatggcg ctgatgcgca aaacctttgt
    17701 cccccgcgag gcctttgaaa aactctccga ccgggttgag cgggtggaga gccggatttc
    17761 cggcctgccc acgcagcagg aggtgaacag actcaatatc gaaatcacca ccctgcgcgg
    17821 tgagcttaag gccaccaatg ccaccctgaa atccgtctcc catcagaacg aactgctgct
    17881 ggagcaggct gtaaggaaaa aacacaatga gtgatttcat tactgaagat cagcgtcttg
    17941 tcattctgcg gtcgctggcg gattacaacg gcgaactggg cgaatccgtg cttcaggact
    18001 gcctggatga ttacggtcac cgcgtgtccc gcgacaccgt tcatacccac attgcctggc
    18061 ttgccgagca gggactggtg cgtaagcgca cgcttgttaa cggttatttc atcgctgaac
    18121 tgaccggccg gggtcaggat gtggcggaag gccgcgccac cgttccgggt gtgaagaaac
    18181 cacgcgcaag ggggtaacga tggataagcc aacgcgtggc cgtgtacgca aggtggattt
    18241 gctccccgac agcatccgta agccgctgct ggaaatgctg cgtgaaaaac gtctgacgca
    18301 ggtccagatc cgcgaggaaa tcaaccgtct gatccgtgaa gcaggtctgc cggaagagca
    18361 gcagctctca ccggcggcca tcagccggga agcctcccgt aatgagctga ttgcccgtaa
    18421 tctgcgtgat ttacgcgagc agacaaaagc catgatggcc gaactggggg ataagccgac
    18481 cggtgaaacc acggcactga ttctggagat gtcccgtgcc ctgatgtacc gccgtctgcg
    18541 tgccgccacc gaatccctga acagcgacag cgatgtggat atgcgcctta ttaaggacat
    18601 cctcctgtcg gcacagcgtg cagaaagtgc cgccgagcgc agcattaagc gtgaaaaaga
    18661 aatccgggcc gcatttgctg aagagatggc gaatgcggtc accgatgagt tgcgcggcgt
    18721 ggatggcatg agcgagcaga tcgaattccg tatcaggcag atgctgctgg gtaaggcgta
    18781 ggaggtgaat catggcaaca gataaccgct cactgatgag cagtgcccat cagaaagcat
    18841 tcagtcttgc aacacagtta cctcctgaaa ccataaaggt atgtcgtgag ttgactgaac
    18901 tcagcattgc cggggaaata tccctggaac aactgcattc gctgctggca gaggctgcca
    18961 ctgatgagtt gagcggcgtg gacgtgatga ctgaagagct tgagtcccga atcaaaagca
    19021 ttctgacagg taaagcatga tgaacaaagc cagagaaacg tcgccttcac tgatgcaaat
    19081 gacgcctccc cgcaaaattg atctgtcagg tgaaaaagcc ctgctgggcg tggatgtgcc
    19141 ggacagcctt gatttgcccg gtgatatgcc ggtgtttctg gattatcagg cccgctggtt
    19201 tgcagatgaa agtcaggtct gcatcgcgga gaaatcccgc cgcaccggtc ttacctgggc
    19261 ggaagccggg cgtaacgtca ttactgctgc aaaaccgaag cgtcgtggtg gccgcaatgt
    19321 gttttatgtg ggatcaaagc aggagatggc gctggaatat atttctgcct gcgcgctgtt
    19381 ctcccgtgcc tttaaccagc tggcagatgc tgatgtgtat gagcagacat tctgggatcg
    19441 ggataaaaaa gaagaaattt tgacctacat gatccgcttt ccgaacagtg gattcaaaat
    19501 tcaggcattg tcttcccgtc cgtcaaacct gcgcggcctt cagggggatg tggtgattga
    19561 tgaagccgcg ttccacgagt cactggatga gcttctcaag gcggcaatgg cgctcactat
    19621 gtggggcgcg cgcgtgcgta ttatctccac gcataacggc gtcgataatc tgtttaatca
    19681 gtacattcag gaagcccgcg aggggcgcaa ggattacagc gtccaccgca taaccctgga
    19741 tgatgccatt gcggacgggc tgtaccgtcg tatctgttac gtcaccgatc aggaatggtc
    19801 accggaaagc gagcagaaat ggcgtgatga tctctacaaa aacgccccga cccgtgagga
    19861 tgccgacgag gaatacggct gtatcccgaa aaaatccggc ggtgcctata tcccccacgc
    19921 gcttattgaa atggcgatga tccgcgacat cccgattctg acgtttgaag ccccggacaa
    19981 cttcatcagc cgtgccgcat ggctgcgtga atcggaagtt ttaacctggt gtgaagaaca
    20041 tttaaaaccg cttttagaga aattaaatcc ccgttcccgc ttcagcttcg gggaagactt
    20101 tgcccgcacc ggtgacctgt cctgctttgt gctgctggaa atcaccgaat ccctggcaaa
    20161 gcgcgaggtg tttcgcgtgg agctgcgcaa cctgccgtat gcccagcagg aacaggtgat
    20221 gatgtacatc ctgacccgcg ttccggcgct ggtcggtgcg gcgttcgacg ccaccggtaa
    20281 cggcggctat ctggcagagg ccgcactgct ggcctttggc ccggatatca tcgactgcgt
    20341 gatgttgtcg ccgaagtggt acggcgagtg gatgccaaaa ctgaaagccg agtttgaaga
    20401 tcagaatatc ctcgtcgccc gccatcagac cacgcttgat gatttgcgcc atgtgaaggt
    20461 agtgaatggt atcccgcaaa tcgacaaggg gcgcacgaag gaccagaacg caacggctgc
    20521 gaacgcccgc cgccatggtg attttgccgt ggcgctctgt atggcaaacc gggcgtcata
    20581 catggagggc ttcatcctgg atgaatcagc ctgtcaggcg ctaccggagc ggtcgcgggc
    20641 aatggagggc ggttatcgtg atgacgatga ggcatatcat gaatttgatc gggggggctg
    20701 gtagtgggac gcatcattga tactgacgga aaacctttct cctttgaccc ggagatgcag
    20761 agtgccgcgc tggatattcc gcagattgcc agccgttata ttgaacatcc ggcttcgggt
    20821 atcaccccga accgggcggc gcagtgcctt cgcggggctg aacgtggcga tctgattgcc
    20881 cagtccgatc tggccgctga tattgaagaa aaggacaccc acctttttgc ggagctgggc
    20941 aagcgacgtc ttgccattca gggggtgcca tggagtattg agccgccacc gaacgccagc
    21001 gcgaatgaga aaaaggacgc ggaaatgctc gacgaatatc tgcattccgc agactggttt
    21061 gatgccatgc tgtttgacgc cacggatgcc atcctgaagg gctattcctg catggaaatt
    21121 gagcacggga tgctcggtaa aatgcacatc atccgcgcca tccgctggcg tgacagcggg
    21181 catttctgcc ttaacccgga tgatttgagc gaactgcggc tgcgtgacgg cagccatgcc
    21241 ggggtggcgt ttcagccttt tggctggata gtgcatcagt cacgttcacg gaccggttac
    21301 ggtggcgcaa cggggcttgt caggacgctt atctggccgt tcattttcaa aaactattcc
    21361 gtgcgcgatc tggccgaatt tctggaggtg tacggactgc cgatgaaggt cgggaaatac
    21421 ccgtccgggg caacaccgga gcagaaaagc gccctgatgc gggcggtgat ggatatcggg
    21481 cgacgtacag gcgggatcat cccggccggg atgtcgctgg aatttcaggc ggcagcaaac
    21541 ggtcaggccg atccatttga aaccatgatt tcgtgggggg agcgttccat ctccaaagca
    21601 atactcggcg gcacgctgac cacggaagcc ggagacaaag gcgcgcgctc gctgggtgag
    21661 gtgcataacg aggtgcgccg ggaaatcagg gattctgatt tacggcagct ggctgcaacc
    21721 ctgaaccgcg atctggtgta tccgctgtac gccctgaaca ccacgcacac tattgatatc
    21781 cgccgtctgc cacgtatctg cttccagacc aaagaaccag gtgatatcac caaaatcacc
    21841 agtgcggtga tgcagctcag tacggggatg gatatttctg atccctggat acgggagcag
    21901 accggtattc cgcagcccac gcccggtgaa gccatattcc gtgtccgtca gagtggcaat
    21961 gaaccggctc agaccgacag ggaaacacca ccggaaaaac aggaaaaaac agagcagacg
    22021 gcgctgtcag ccagactgcc ggaagcgaaa agcacgccgc gtgatgaact ggatgatatg
    22081 ggggacgcgg tgcctgcccg ccgcttacag gaggccatcg acccggtact ggagccggtc
    22141 attgatgcca tcagaacgcg ggggctggcg gatgcgctgg cagacctgcc tgccctttat
    22201 cgtgaaatgg atgattcccg cctgatgacg ctgctcagtg atgccatgtt tgctgcggaa
    22261 atgaagggga tgctggatga cacaggggat tgatttaggt tatgccgcca cgcttccctc
    22321 aaaagaggcg gtagcatact tccgtgtcaa aggggcgcat atcagctgga actggttcga
    22381 aacagccgcg gatgttcatg cccgctcatt cacggcagca aaagcggcac gcctggatgt
    22441 ggtgaccacg ttacaggccg aagtgcagcg ggccattgat gagggaattt cacagaaagc
    22501 atttatccgc acgctgacgc cacgcctgca aaagctggga tggtggggaa agcagattgt
    22561 ggtggacagc gacggtaacg cggaagaagt gcaactgggc agtccccgtc gtctggcgct
    22621 gatttacaac gtgaacaccc gtgtggctta caatgccggg cgttacacgc agatgatgaa
    22681 caacacggac acgcatccgt tctggcagta tgtggcggtc atggacagcc gtacccgccc
    22741 gtcgcattcc gcccttaacg gtctggtatt ccgctatgac gatccgttct ggaaaacaca
    22801 ctacccacct aatggctgga actgccgctg ccgtgtgcgg ccattgtctc aggctcgtct
    22861 ggatgcaatg gggttatccg tttcatccgg tgaggatcat ctctccaccc gcaatgttga
    22921 ggctggcgtg gataaacaga ccggagaagt cagagaaatg ccggtgacca catattcaga
    22981 tggcaccaga accatgacac cggatgtggg ctggtcatat aaccccggct cggcagcgtt
    23041 cggcacagac caggcgctga tccgcaaact gatcgaggtg aaaagcccgg cgttacggga
    23101 aatggtggtt caggagatga acaacagccc ggagcggcag ctggcgttcc gcatctgggc
    23161 aaaaaacatc atgcagaccc ggcgaggtgg taacgatatc cgcacgctgg gctttatgac
    23221 tgaaagcatt gcgcaggcag tggaaagccg gacgggaacg ccaccggccc gcctgctggc
    23281 gatgagcggt aaaaatgtgc tccatgcaga cagtgtgaaa catcagaatg acggcatcgc
    23341 cctgatgcca gaggacttcg ggcgcttacc ggcaatgctg gcaaagccaa aagcggtgct
    23401 gtgggacaaa cggcacaaaa atctgatgta tatcgtggaa aataaggatg gcagtgtgca
    23461 gattgccgtt aatgcaccgt acagtctgaa acgtcagccc gacaaactgg atgtgattgt
    23521 gaatgcttac cgggttatca atatggataa gctgaaatcg gatatccggg gcggaatgct
    23581 tgaggtgctg gaaggacaaa ttgattaacg gtcagtggcg ggggtcgaac ccgcatacat
    23641 gatccacccg gaggcaggca ccgactttac cagttaagcg tacactgacc gcctgtttat
    23701 tttatattca tcctttatgc ggaggcaata tgtcatccat tgatgccgct gtcgttgttg
    23761 acgttacgcg tctccagcgg gtgtttgccc ggcttcagtt tgtgggcggc ggaaaagacc
    23821 tggcccgcag tgtggcggag agtcttctgt catcgtcaga aatggcgttt gaacaggaaa
    23881 aagagccgga cggcgaacgc tggcatgact ggtcagatcc ttaccgcaag tggcgtaccc
    23941 gtaagggata cacgcccggc aaaatcctga cactgaacgg cgatctggcc cgccgcctga
    24001 ccacggatta tggcgatacc tgggcgctga ttggatcaaa tgaaccttat gcggccattc
    24061 atcagtgggg tggcctgcct ggtatgccac cgggaccggc ggccattggc gcacgtccgt
    24121 atatgggctt tgatcaggtg gcagaacagg agatcatgga cgaaatcaga aaacgcttta
    24181 aaaaggccac agaaacgccg taatggttta aggtatacaa acgcattacc ttaccctctt
    24241 caggcgcgtg tcgtgatttt ctaacctgta tttaacgggc tttaaaatct gcgtgaagcg
    24301 cctttctcat ttttcccctg tcagcacttt ctgaagtccc gcaaaaccgg ccccgaaaaa
    24361 caccggctta tgctgccgga atgaagacga aaaacacgcc caaacttgct tatgccattc
    24421 tgaatgccat cagcctgtcc gcagacgggg acggtgactg gtgtcagatc atgccagccg
    24481 gtcgggtaaa ggcccgtgac ggtcgcccgg aaaaaccggc ggaaggctgg ctgattaacc
    24541 atgcggccgt tgagcgcatg gtatcccggg ttgtggcgct caatcagccg gtaaaaattg
    24601 actacaacca ccagaccctg attaaggggc atccggcacc ggctgccggt tttgtcatgg
    24661 cctcaccgga aaacttccgc ttcagtgaag agcggggttt tgaagtgcgt ccggactgga
    24721 accctccggc cattgaacac ctgcgcaata acgaatttcc ctggttttcc ccggtgattg
    24781 gttatgacga aatcaccggt gaacctgttg agcttcggat gctggctatt accggtgacc
    24841 ccggtctgac tggcatgaat cctgtcgccg cactgtcggc ggatgacctt tataacgcct
    24901 taaaccctcc tttaaaggac acctccatga atgagcaatt acgccagttg ctgacggcgc
    24961 tcggtctgac cgtggccgac ggtgacgaat ttacaccgga actgggcacg gcggcgctgt
    25021 ctgcccttac cgggatcaaa acccgtgcgg atgcgcacga caccctgaaa acgcaggttg
    25081 ccagcctgtc ggcagagctg gaaaccgcaa aaggcacacc aactggcggc actattgatc
    25141 tgacgaaata cgtgcccgtt gagacgtata acgccctgcg taccgaatat gtcgcgttgt
    25201 cggcacaaca cggcagcacc acgctggagc agttgctgga taaggccgag tccgaaggac
    25261 gcattttcaa aagcgaacgc ggatacatgg aaggactcgg acaacagatt ggtgttgcgg
    25321 cactttctgc acagcttgac gcccgccagc cggttgcggc tctgaccact cttcagaccg
    25381 acaccgtaac cgtgccggat aaaaagaccg ccaccgctgc gctgtcggct gaagatatcg
    25441 ccgccgccca tctgctgggt aaaaccgaag ccgagttcct gaaaatgaaa gaggaaatgc
    25501 aataatgcct accccgatca caccggcgat gatcaccgcc ctgatgacgg gctaccgttc
    25561 tgattttcag gccgggatgt ccatggctcc gtctcagtac cagaaaattg cgatgaccgt
    25621 accgtccacg tcgaaatcca acacctacgg ctggctgggg cagttcccgc agttccgtga
    25681 gtggatcggc tcccgcgtca tcgagaagat gaaagcctat ggctacgcca tcgtgaacaa
    25741 aacctttgaa ggcaccgttg ccattagccg cgatgacttt gaagacgaca atcttggcat
    25801 ctactccccg ttgttccagg agatggggcg cgctgcggcg gcacaaccgg atgaactggt
    25861 ctttgctgca ctgcgtgatg gtctgaatgc ggcctgctat gacggccaga acttctttga
    25921 taccgaacac cctgtttacc ccaaagtgga tggctccggt gatgcacaga tggtcagcaa
    25981 tatgtttgtg gcaaaaaccg gctctgtcgg tgcacaggcg gattacagcg gcccggcctg
    26041 gtatctgctt gactgctccc gcgcggtaaa accgctgatt tatcaggatc gccgcaaggc
    26101 cgaactggtt gcccagacaa aagtcgatga aggccgtgcg tttactgata acgagtttgt
    26161 gttcggtgct tccgcccgtc gcaatgtggg ctacggcttc tggcagatgg cctacatgat
    26221 gcagtcaccg ctgacgctgg atgcgctgtg gcacggctgg tcagccatgc gtgactttaa
    26281 ggctgacggt ggccgcaaac tcggcatcaa acccacccat attgttgtcc ccacctcgct
    26341 ggaaaaacag gcggtgcagc tgctggagcg cgagctgttc gcggacggta acaccaccgt
    26401 ctctaacgag atgaagggca agctggagct ggttgtcgcg gattatctgt aaacggtgag
    26461 ccgggcttac ggcccggccc ctgcggaggg caaatatgaa tgaacatcac actgtggcag
    26521 caacagatga ttcaggcctt caggtcagtg gtgacagtcc tgtcacggta ctggcagaag
    26581 tgcgttgcag tcgtgcgcag tttcgtcgtg cggggttcct gttcatgcgg ggacgccagc
    26641 aggttgacgt caccccggag cagcttgccc gactggaagc ggagccatgc ctcactgtac
    26701 gaatactaca gacgcctggt gatgatgcgg gagccgtggc gggtgtggtt catgcagaga
    26761 ccggtgcaga tttagccgaa gccggacagg atgctccccg gaaaaagacc ggcaataacg
    26821 ccagacaggc gaaaaaggct cgcgcatgaa ttacgccacc gaaaccgata tgcgggcacg
    26881 ttaccgcgag gatttgctca ggccattact ggccgtgccc cgttcggatg agccggacgc
    26941 gcgcaagctg aaccgggcac tgacggacgc gtctgccctt atcgacagtt acctgtctgc
    27001 ccgttacacg ctgccgctgg cggtggttcc ggcggtcctt gttcagcatt gctgcacgat
    27061 tgccttttat tacctgtgcg atcagcgggc atccgatcag gcgcgtgacc gttaccgtga
    27121 ggcgctggcc tggctgaaag atgtcatgaa cggtaacgtg ccggtcggcg tggatacaga
    27181 cggtgcggcc cctgaatccg gggatttacc gcaggtgcag tctgatgcgg cggtgttcgg
    27241 gcgcaaccag aagggcttta tatgattacg gaaaccgagc aagcgtacat cgcccgtatc
    27301 cgtgagtatt tcgggaatga actggtgtca gttgacacgc atcccggcga ctggagcgac
    27361 agtgtgctgc gcaccatgct gattagcgcc ccggcgatct acgttgcctg gctgggtgcc
    27421 ggtgaaggcc gtacccgtgg gcgtctggtc agccactggg tgttctacgt catcggcgac
    27481 atgctcaatg gccgtgaggt cagccgtccc gggctttatc agattgtggc ccgcctgatt
    27541 accgtgctgg acggcttcag aaccgaaaaa acctcaccgc tttactttga aaaggcggtc
    27601 aacggttaca ccgaaacgca ggccgacagt ggcgcggtga tgtatgcgct ttatttctcc
    27661 tgcgaggaaa tgatcgcacc gctgactgat atcagctcgc tggatgattt cctgcgccat
    27721 tacgaaacct tcaccgaacc ggaaggcaca ccggaattta aggcgcacat cagcctgcca
    27781 ggtgcaaccg ccgccggtga aaaagccgga ccgtcagagg aataacagat gacaaccatt
    27841 tttatcaaac ccgcgccggg atgcctgatc cgcgatccgg acaccatgaa accgctggcg
    27901 caggagggag aggaaaaacc cttcaccccg ttctggtgcc gccgtcttaa tgacggggac
    27961 gtcattgtgg tggaaaaagc ggctgaagca gcaccggcag cagcgtctgc aacgactgat
    28021 gccgccggaa aacccgcggg agcggcagca gaaaaacctg cgactgaaaa acccgcgact
    28081 gaaaaacccg cagcatcaga caaggggacg tcctgatgat taactttgat tatatcggcg
    28141 ataacaatcg cattccgctg gtacagattg aaatcaataa ttcaatggcc gtgaccggca
    28201 ctccaccaca gcgtcaggcg gtactgttgt ttggtcaggc tgcaatgaaa gacaccacca
    28261 ttcaggggcg cggtcagctt gatgtaccag tacgtattac ccgagcttca caagcaagag
    28321 aactctgggg gcgaggttcc atgattgcac tgatggtaga ggaatttatc gccattaacc
    28381 ctgacacaga gttatatgcc attgcacagg gggctggaac aggtcagtcc agggcatgcg
    28441 tgatgaatat cattggaaca gcattggcag acggggtgtt aagtgtttac atcggcggtc
    28501 gccgttacct cctgcccgtc agtaagggga aaaaaggtaa agagcttgtg gaagagctgg
    28561 ctaaggtcat taacgctgac actgatgcgc cattcactgc tatcgcagct gaagtcagtg
    28621 gtgataatgc tgaaggactt aaagggagca tgggggttaa cgcccgcttt atcggtgaat
    28681 gctcagctca tgatttacgg gtgaactatt atgacggtga aactacaccg gccggtctta
    28741 cgctggagct cgtcgcgcca acacagaagg caacaaaccc cgatatcacc cgtagcgtct
    28801 cagggatggg gaatcgtcag tacaactaca tcgtcatgcc ctataaggat gcggcaaatc
    28861 tcaaaattct ggcggatgaa ctgctgaaac gctggggacc ggtaaaaatg tcggacggta
    28921 tggtctggat ggcgcatacc ggcaccttcg gtacggcaac cagttttggt gaatcccgta
    28981 atgacttcct gtttacctgc acttctattc ctaaagcacc agagccggat tatgtctggg
    29041 cggcctccat ttgtgcggtc tgtgcgccct ccctgtcaac ggatccggcc cgaccattac
    29101 agacactggc gctgcctgcc cgtatggcac ctgaagcacc ggctcgtctg acgcgggaag
    29161 agcgaaacag cctgctttat accggcatgt ccacggtgac cgttgcggcc gctgatgtgg
    29221 tgcagattga acgtcaggtg acgatgtacc gtcagaatgc atacggcgaa agcgatccca
    29281 gctatctgga tgtagaaacc atctacaccc tgtcatatct gcgttattcc ctgcgcacct
    29341 ttatcacgca gcgtttcccg cgccacaaac tggccgataa cggtactccc gtccggcccg
    29401 gtcagaatat cgtgacgccg gagattatga cgctccagct gattgcactg ggggaagaat
    29461 gggtggaact cgggctggtc gaaaatctgg acacctttaa aaagaatctg ctcgtggagc
    29521 gcaacacaaa agaccgtaac cgtctggatg tgatgtgtac accggatctg gttaaccagt
    29581 tccgcttcat ggccgcgcag atccgtttca ttttgtgagg taaggcatga gcggaaaaca
    29641 gcatcagggc acggccacca tccgtgtgaa cggacaggag tacgccaccc ttgagggagc
    29701 cacgttctcc ccgtccggct ttgagcgtga agtggtgaaa ggcgcgaaag tctatggcta
    29761 tcgtgagaaa ccccgtgagg cgacgctgga ctgtaaattt ccggctggcg gagaaggttc
    29821 accggctgcc gatgaaatca acacctggac tgcggtcacg attgagttcg tggccgatac
    29881 cggtgaagtc catatgatga cgaaggcctg gagcagtgaa ccggcctcgc ttgacggtgg
    29941 cggtgatatc tccgcgaagt tcgccagcgc caccagtacc cgtgtccagt aatcaggaaa
    30001 aatgcaatga ccacacgtaa gaaaaaaacg gcggtttctg acgaggccgt gatggaggct
    30061 atccgggatg tacttgaaga cggcgatccc cgcaccgccg ggctggctga acagctggcg
    30121 aacggctatg tggatctgct cgacggtctg ccgttcgggg aatcccgtga ataccgcgtc
    30181 accttccgcg aactgacggc gaaagacagc attgacgcgg aaaccgaggc cgagcgtctt
    30241 atggaaaccc gaaatggcgg cccggttctg atagcctcgc cggcactgcg cggcattgca
    30301 ctgctgcgcc gtcagattgc cgctgttggc cagattgagg ggccattgtc tccgcgtcag
    30361 atcggacagc tcagcgagcg cgatctctcc cgtctgatgg cggcggtaag cctgcttgat
    30421 tccgcgatgg caggaaagct ggtcgctgaa cgggggcgac cagatgcagt gccgggatca
    30481 gattgaagaa gccgcaattg tgctggggat ggtgacaaaa agcggcccgg agtgggcgct
    30541 taacctcccc ttatcgcagc tttaccggca ctgccgacag accgaaaaaa ttctcagaac
    30601 gaagcagtaa cccatggcaa aaaacctgaa agcctccctg attgtcgatt tactcggcaa
    30661 tatttccgcc aaatcccgcc agtggtcaca ggagctgggg gcattctccc gttccggcag
    30721 gacggggctg ggtggtcttg gaaatgcagc acgccgggcc ggtcaggaaa cagagcttgt
    30781 tggctcccgt atgcagcgaa cgcttgccgg tgttcgtggc agtatccgca cggtaacgtc
    30841 agattttgac cgcctgcagg gcagcattac cggcaccatc gggcggataa gcaaccttta
    30901 cgggatgctg gcgggagggg ctgcggtcta cggttttaat aaagccttta tccgcccggc
    30961 cgcagagatg gagaactata ttctgcgtct caacgccatt aatcatggcg ataaggcaaa
    31021 aacggaagca gtgaaagcct gggcggtaca gaacgccaaa gacaccacct gggggctggc
    31081 gggggttatg caggaatacg cctccagtcg cgggtttggc atgagtgaca gggaagcccg
    31141 tcgctttatt accatgcttc aggatcaggg cggctatcac ggctggtcac tgtcagaggc
    31201 acagggtgcg tccctgcaac tcaaacagat gtttgcccgc cagagtatcc aggcagcgga
    31261 cgccaatatt ctgaccggat acgggattaa cgtttaccag ttactggccg ataagctggg
    31321 tgtaaaccag aagatcatcc gtgagaaagg cgagaaagga aaactgggac cggacagcat
    31381 tcgcctgtta tttcaggtga tggccgaaca ggcaaaaggt gcccagaaaa acgccatgaa
    31441 ctcatggacg ggaatgacgt ccatgatggg tgacgtctgg gatcagtttg cccgtgacgt
    31501 gatggcgaag ggaccgtttg acagcctgaa aaagagcctg aaaggctttc tggattatgc
    31561 cgatgcggca cagaaaagcg gcttacagga taagctggcc acacagacgg cctcagcact
    31621 gaatcaggga tttgagtatg ccagagatgc tgcgaccggc ttttaccggg ccattcagaa
    31681 agtcagggaa acgcttcagg cactgcgcga cgccggttat ggcgatgcgc tggatcgtat
    31741 tggtcagggt gcgcagaccg ccgcaaagta cctgatgtat atgtatctcg cctcccgtgc
    31801 gctgaaggtg ctcaggtttg cagggacggg ggcattacgt ctcggcgcaa cccccttacg
    31861 ctatggcatg gcgatgacct ccgtgctgac gtcgcccttc cgcaaaccgc agaccactgt
    31921 accgggaacg cagcccggac gcgctggccg gttcctgaat ttccttaccg gggtaaatcc
    31981 ggctgccgtt cagcccgtgc tggtcaccaa ctggcctgcg ggtggcctgg cctcaggtgg
    32041 cggggatgtg gtggtcagcg gtgacggcaa aaccgtgcgc ggccgtaaaa aacgcggccc
    32101 cggacgtgga cggggtgtca cgaccgtcgt gactgccggt gagcaactgg cggagagtgc
    32161 cggtaaacag ggcttcttcg gtcgtatgat gagccgtgcc ggtggtctgc taacggcggc
    32221 cggaaaccgc atggggctgg ggcgttttgc cgggctgttc cgtggtgccg gtcggctggg
    32281 tggtggtgcc ctgtgggccg gtgccatggc ggctcctgta ctgctggaca gcagcgccag
    32341 tgcagccgat aaagccggtg ccgtgggttc tcttgccgga agtatcgccg gtggcgcgct
    32401 gggggcggct gccggtccgg tcggggttgc cattggttcc acggtgggca gctatctcgg
    32461 tgattatctg gggggctggc tgacgcaggc ctggcagaaa ctgcgtggca gcagtgacga
    32521 aaacggcgga caggccaccg cgaaaaccgc cgcacgggtg gagcttgtgg cccctgaagg
    32581 ctggcgggcg cgcagtattg atgtggatga taccgcacag catggcctgg atgtgaacgt
    32641 ctggaacgga gggaactatg gcctctactg acacgccggg gcgtggctcc tttcgtggcg
    32701 taccgttcct ggtttatcag gagcagcggg aacggggcgg acgtaacatt gtgcgccgtg
    32761 aatacccact gcgtgaaagc ggcggtgcgg atgatctggg gccaaaactc ccggagttca
    32821 cattcacggt tctagtgacc ggagacgatc ttcagacaca gcgaagccgc ctgcgtgatg
    32881 cgctgcgtgc acccggtgcc ggtgaactga tgcacccgga ttacggcacg ttaaacgtgc
    32941 tgataaacag ctttgaaagc cgttataacg ccagcgagca gggaacggtt gagttcacga
    33001 tcaacgtgat ccccgccagt gacgataccg ccccttcggt ggcagaagat acggcagctg
    33061 cgctggagca gaaaagcggc tcagcaatga atcagctgtt taacacactt tcagacggct
    33121 ggacggtgat ttcagacggt ctgcatgatg tacaggccat gaccgacacc atcagcgata
    33181 aaattgatgc gctggagaat gccgtttcag ggatggggat tgtgcaggat atcagtgcct
    33241 ttacggccag ttttactgcc ctgaaaggga atgccgccgc cctgattaat gcgccccgtc
    33301 gcatggctga atcgctggcc ggaatgtttg ccgtgctggc ggggctgccc ggcgatcctt
    33361 ccctgtcgct gaccggaaaa gccggaacgc cgtccggcag cctgaccaca aaccgtgaca
    33421 gcacgacaga gcaggccatg ccgcagctgt accgcacact ttcttccctg cgttacaccc
    33481 tcagcgaaca ggatgatccg cagcgcctga ttggtctgac gcctgcggca cagaacaata
    33541 tccgtctgct gcggagcgtc atgcagagtg cagccctggt gtcgcaggcg cagacggtcg
    33601 gaaaactgct ggatcaggtc atccggcaga atacgacgca cgttcaggat actgaccgtg
    33661 cgggcggact ggcctggctg gaaagttctg ctgatgtgca gcgtatcaac cgggatttga
    33721 gcgatgccat ggaacaacag gtgctggatc tgtccgcaca gggccatacc ggcaccgccc
    33781 ttgcattacg tgatgcaggg cttgcgctga cagaagattt aaccacgcgg agcgtgcatc
    33841 ttcccggtgc ttttcaggtc atggtgcgaa ccaccgaacc ggcactggtc acgctgtacc
    33901 gtgccaccgg taacagccgc cgctggcagt attttgtccg cagaaacaat atccccgatc
    33961 cggtttttgt tccgggtggc cgccgcgtgg aggtgatcag tgagcagcag gattgaactg
    34021 tatatcggcg gcagtatttt ttccggctgg ctgacggtca gcgtccgtcg ctcgctggag
    34081 catctggccg ggtcgtttga actggggctg atgctgcccg gtgagcgcat cccctcagcc
    34141 ctgcggaccg gtcagtccct gacgctcaga attaacgggc agacggtcat cagtggctgg
    34201 ctggatcagg tcagtcagcg gatcagtgca acccgtcatc agatcagcat cagcggtcgg
    34261 gataaaaccg gcgacctggt ggactgtgcc gccatccatc ccggcagcca gtggcgcaac
    34321 cggacgctgg cacagattgc cgcagattta tgtgcgccct tcgggatagc cgtgcgctgg
    34381 caggttaacg acgacacggc tgcacgcccc ttcagctctt ttacgctgga aaactcagaa
    34441 accgtggcgg atgcgctgac ccgtgctgcc cggcatcgcg gggtgctggt gaccagtaat
    34501 gctgacggcg atctggtgtt cacccaggca ggaagccagc agacggacag actggtgctg
    34561 ggagataacc tgcttgatgc cgattacaac acggactggc gaggacgata cagcgaatac
    34621 cgtgtccggg gacacgggcg cggtggtggc aaacggggag acagcgaatc cgccgcccgt
    34681 ctggcagcac ctgtgggcgt catcagtgat gagcagatcg gccgctaccg gccgaaaatc
    34741 atcctcgccg atcaacagac agacatcacc ggtgcacggc agcgtgccct gcgtgaaatg
    34801 cgccgtgcga ttgcccgttc agaacggttt tctgccaccg tgcgtggctg gttccgggat
    34861 gatggccggt tatgggatgt caatctgctg accggtgttt cagccctgcg tttcggtata
    34921 gaacagactg aactgctggt ctgtcaggtg gagtttttac ttgatgagca taacggggaa
    34981 gtcacccggc tggtactggc accgcgtgac ggctttatcg ttccggcaga gccggacagt
    35041 aagggccggg gtggttccgg tgacgatgtt gatgccttta ttcgccagca gatgaaaaaa
    35101 caggggatca gctttgatga atgatgaagt gttcagccgc ctgattgccc cggtaacgcg
    35161 cggtattcgc ctgttgtttg gccggggcgt tctgaccggc acacatgacg aactgaaaat
    35221 gcagaatgtg cagctcaccg gcatggacgg cgaaaccttt gatgatgtgg agcgtcccca
    35281 gcagtacggg cagatcagcg ttcccctgcc gggtgcggaa accttttttg cctgtctggg
    35341 cggacagcgg gatcagactg tggtgcttgt ggtggaagac cggcgcagcc gcccgaccgg
    35401 actcacagcc ggagatacgg gggtgtatca ccatgagggg caccggatac ggttaacaaa
    35461 ggatggccgc attattgtga catgcaaaat gctggagatt tacgccgacg aggggatgcg
    35521 ggtggatacg ccggaagcca cctttacggg caatgtgacg gtggataaga acctgcatgt
    35581 taagggtaat ttcgcgcttg agggtacggg gaaatctcag ggactgttca caatgtcgga
    35641 tgccgttatt gcagggatac ggtattccgg ccatgtgcat catgataacg gcgaaggcag
    35701 taagacagga gtgccggaga atggctgata ttgcaattgt gtgggatcag ggatgcggtt
    35761 cgctgcaact gaacggtgcc gacctgctga cggatgacag tctgctgacg gccgttctga
    35821 tttcgctgtt tactgaccgc cgggcgctgg catcggatga aatccctgac ggtacgcgcg
    35881 accgccgggg atggtgggga gacagttttc gcccgcgccc cattggctcc cgtctgtggc
    35941 ttctcagccg ggaaaaaacg ctggcctcgg tgataagccg tgcccgtgct tacgcggatg
    36001 aggcgctggg ctggctcagt caggatggcg tggcgtcatc cgtggtctgt catgcagaac
    36061 gtgtgggaca cgcgcggctg gcgctttcgg tgcgcataac cctgcctgat ggttcagtga
    36121 ggcccatgat tttttatgct gatcttaagg gggagtaatg ccttatcagc ctttaccgct
    36181 ggcacagtta atcacgcaga cacagcagga tatcagccag cgcctgcccg gctcgcagcc
    36241 gggtgtgaat gaaaccaccc tgaatgccat tgcttacgct caggcagggt tatctgctca
    36301 ggagcatgag catctggcct ggattgcgcg tcagatcatc ccgaccgaag ccgatgaagc
    36361 cgaactgctg aaacactgtg cattctgggg cgtcatgcgt aagcctgcct ctcgggggga
    36421 cgggccggtt cagctgatgc tgaccacgga tgcggggatc aaggaaggtg tgcttctcca
    36481 gcgaagtgat ggcgtggttt accgtatcac cacctccctg accggtaagg ccggtacgct
    36541 gaatgtcagt gtggaagccg aaagtgccgg tcgtgcgggg aatgccccgg cagggacaaa
    36601 actgacattt atcacaccgc aggcggggat caaccagacg gccacggtga ccggaacggg
    36661 gatcaccggt ggtgcggatg tggaaaccgt gccggagctg ctttcccgtc tggttttccg
    36721 ggtgcagaat ccaccgtccg gcggcacgca gtatgatttt gaacgctggg cgcgtgaagt
    36781 gccgggcgtg acgcgggcat ggtgccgccc ggaatggccg caggcgggca gcgtgggggt
    36841 aacgtttgtt caggataata acccggatat tttccccggt gacggtgatg ttcagcgggt
    36901 ggcggattat atccgcagtc atgatgatcc ggcgaccggc cagcctgtcg gacagcctct
    36961 ggggccgacg gtgaccgtgt ttaaactgac caataagccg gtgcccttca gcatcaggat
    37021 catcccgaaa acaccggaga atcaggcggc cgtaaaacag gcgctgaccg acctgttgta
    37081 taacgaatcc cggcccggcg gtctggttct gccgtcgtct ttctggcggg cggtggcagg
    37141 ggtgaaaaat ctggaggatt ttgaagtgcg cagtccgctg acgtcagttc aggcgggaga
    37201 cagtgagctg ctgacggtag gagaaatcac atggctgtaa ccctgacccc gcatcagcgc
    37261 gccctgttgc agttactgcc tgacgggctg gcatgggaca agcgtccgtc atccgtactg
    37321 gcatcattat gtctgggcct cagtcattcg acagcgcgtg tatcctggac gggtaaacag
    37381 ctgcttgccg agcgtttccc tgacacgtcg cgcctgctgc tggaagactg ggagcgttat
    37441 ctcgggcttc ccgagtgtga tatggccgga gccaccctca cggagcgaca gcgttatgcc
    37501 ggaaataaat accggatgaa gccctcgctg aaccgtgaat tttatatccg gtttgcggcg
    37561 gagtttggtt atcagattga cattcagccc tcaccggatt cgcagtgggt cagtattgtc
    37621 acgattaaca gtgaaaccgg ctaccggaat atgaatgtgc tggatgatat tctcacgccg
    37681 ctgcgtattt atgaaggcgg tgcgctggaa tgtattctga accgttataa gcctgcatgg
    37741 cagacgttta tttacgttta tgcaaacagc catgaagagg agactattta atgtttcatg
    37801 ttgataataa ttccggcgtg gcgaatatgc ctgcgctggc accggcgcag agtaatacca
    37861 ccacctggtt taccgaaggt gacggacaaa aaggtatcag ctggattggt caggactggc
    37921 tgaatattct ccaggccgaa ctgctgaata ttctggctga agccagtatt cagccggata
    37981 aggcgcagtt aaaccagctt acgctgtcca ttaaagccat tatcgctgcg aatgcctttt
    38041 cccggaaaaa taacctgaaa gaaattgctg atgccggtgc ggaggcccag cgtctggccc
    38101 gtggttatct tggtctgggg acgcttgcca caaaaaacag tcttggtccc gctgacgtta
    38161 atgccctggc gaaggatcag aatctcgccg acctggagaa taagggaacc gcccgtaata
    38221 atctggatgt ttacagcaaa agcgaaggtg ataaccgtta cctgcgcagg gagcagaacg
    38281 gcgcagacat tccggataaa ggggctttta tcgataac
//
LOCUS       DAJTVE010000027        37285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000027 DAJTVE010000000
VERSION     DAJTVE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OO439_001299"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OO439_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO439_001300"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO439_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6315706.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO439_001301"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO439_001301"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6315707.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OO439_001302"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OO439_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OO439_001303"
     CDS             complement(4104..4388)
                     /locus_tag="OO439_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315708.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO439_001304"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO439_001304"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6315709.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OO439_001305"
     CDS             complement(5289..6497)
                     /locus_tag="OO439_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315710.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OO439_001306"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OO439_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6315711.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OO439_001307"
     CDS             complement(7292..8977)
                     /locus_tag="OO439_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6315712.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OO439_001308"
     CDS             9247..9624
                     /locus_tag="OO439_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6315713.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO439_001309"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO439_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6315714.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OO439_001310"
     CDS             10071..10358
                     /locus_tag="OO439_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6315715.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OO439_001311"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OO439_001311"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6315716.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OO439_001312"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OO439_001312"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6315717.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OO439_001313"
     CDS             12115..12591
                     /locus_tag="OO439_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6315718.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OO439_001314"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OO439_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6315719.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OO439_001315"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OO439_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6315720.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16245
                     /gene="potH"
                     /locus_tag="OO439_001316"
     CDS             15292..16245
                     /gene="potH"
                     /locus_tag="OO439_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6315721.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16242..17087
                     /gene="potI"
                     /locus_tag="OO439_001317"
     CDS             16242..17087
                     /gene="potI"
                     /locus_tag="OO439_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6315722.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17147..17635
                     /locus_tag="OO439_001318"
     CDS             17147..17635
                     /locus_tag="OO439_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6315723.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17676..18803
                     /gene="rlmC"
                     /locus_tag="OO439_001319"
     CDS             17676..18803
                     /gene="rlmC"
                     /locus_tag="OO439_001319"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6315724.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO439_001320"
     CDS             complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO439_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6315725.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO439_001321"
     CDS             complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO439_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6315726.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO439_001322"
     CDS             complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO439_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6315727.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO439_001323"
     CDS             complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO439_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6315728.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO439_001324"
     CDS             complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO439_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6315729.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23087..23602)
                     /locus_tag="OO439_001325"
     CDS             complement(23087..23602)
                     /locus_tag="OO439_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6315730.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24251..26020
                     /locus_tag="OO439_001326"
     CDS             24251..26020
                     /locus_tag="OO439_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6315731.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26231..26554
                     /locus_tag="OO439_001327"
     CDS             26231..26554
                     /locus_tag="OO439_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6315732.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26551..27381
                     /gene="amiD"
                     /locus_tag="OO439_001328"
     CDS             26551..27381
                     /gene="amiD"
                     /locus_tag="OO439_001328"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6315733.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27378..28391)
                     /locus_tag="OO439_001329"
     CDS             complement(27378..28391)
                     /locus_tag="OO439_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6315734.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28490..29920)
                     /locus_tag="OO439_001330"
     CDS             complement(28490..29920)
                     /locus_tag="OO439_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6315735.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO439_001331"
     CDS             complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO439_001331"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6315736.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO439_001332"
     CDS             complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO439_001332"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6315737.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO439_001333"
     CDS             complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO439_001333"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6315738.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO439_001334"
                     /gene_synonym="priS"
     CDS             complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO439_001334"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6315739.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO439_001335"
     CDS             complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO439_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6315740.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36990..>37285)
                     /locus_tag="OO439_001336"
     CDS             complement(36990..>37285)
                     /locus_tag="OO439_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6315741.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         9288 a         9218 c         9634 g         9145 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgtgtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgcgctatt
    15481 ccaccttata ccgaactgat ggagtgggct gacgggcaac tttccatcac tcttaatctc
    15541 ggtaattttc tgcaactgac cgacgatccg ctctattttg atgcttatct ccagtcgtta
    15601 caggtggcgg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    15661 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    15721 tggacctcgt ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg
    15781 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tctgcatacc
    15841 aatctggccg tttatatcgg cattgtttac gcttacgtgc cgtttatggt actgccgatt
    15901 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15961 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    16021 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    16081 ggcccggaca gcatcatgat cgggcgcgtg ttatggcaag agtttttcaa taaccgcgac
    16141 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    16201 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    16261 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    16321 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct
    16381 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    16441 taagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    16501 cggtggtgct ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca
    16561 ccgcgccgct ggtgatgcca gatggcatca cgggcttgtc gctgttgtta ttattcgtcg
    16621 cgcttgctca tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc
    16681 atgtcacttt ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg
    16741 atcgctcgat agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16801 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16861 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16921 cgatgctggt cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa
    16981 cgttaattct cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggttcgcg
    17041 cagaaaaaca gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt
    17101 tgccaacctg gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac
    17161 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    17221 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    17281 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    17341 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    17401 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    17461 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    17521 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    17581 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    17641 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    17701 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    17761 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17821 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17881 aaccactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17941 tttatcctgc ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg
    18001 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    18061 aaagccagag cgatggcggc atgatgctgc gctttgtact gcgttctgaa accaaactgg
    18121 cacaactgcg taaggcgctg ccgtggttac aggaacaact accgcaactg aaagttatta
    18181 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    18241 ctgaacaaca ggcgctggca gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    18301 gtttcttcca gactaatccg gcggtcgcca gccagctgta cgctaccgcg cgcgactggg
    18361 tacggcagct gccagttaaa catatgtggg atctgttctg cggtgtgggt ggctttggtt
    18421 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcatca gaggccattg
    18481 cctgtgcaaa gcagtcagcc gctgaactgg gcttaacgcg tttgcaattt caggcgctgg
    18541 actccactca gtttgccacc gctcaggggg atgtgccgga gctggtgctg gttaacccgc
    18601 cgcgccgcgg cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta
    18661 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    18721 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtga
    18781 tgacgctgct ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt
    18841 gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa
    18901 atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg caatcttact
    18961 tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg atatgtaccg
    19021 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    19081 cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca
    19141 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc
    19201 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    19261 gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc
    19321 ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg
    19381 acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg
    19441 atatccatac cggagattac ggagtcatat tttctgaatt tcagtgacgg gatcaggctg
    19501 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    19561 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    19621 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    19681 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    19741 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat
    19801 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19861 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19921 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19981 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    20041 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
    20101 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    20161 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    20221 gaagagagcg agcgtttaaa ggcatacggc agcaggagcg ccagcgtatc ttttttgctc
    20281 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    20341 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    20401 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    20461 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    20521 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    20581 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct
    20641 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    20701 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    20761 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca
    20821 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20881 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20941 agatagccga tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg
    21001 ctttcaacgc gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    21061 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct
    21121 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
    21181 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
    21241 ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg
    21301 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    21361 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    21421 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct
    21481 cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca
    21541 ctttgtcgcc caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg
    21601 tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    21661 tgtcatacgg aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg
    21721 tcccgttctg tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21781 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
    21841 cacgctccgg agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc
    21901 ttgggatcag gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac
    21961 acagcgcttg tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    22021 caaacggagg ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    22081 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    22141 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    22201 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    22261 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    22321 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    22381 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    22441 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    22501 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    22561 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    22621 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    22681 gcctgcaatg ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag
    22741 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22801 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22861 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22921 tttgacagaa taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc
    22981 aataaacata gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct
    23041 cacgaaatgc cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga
    23101 tggactgacg gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgcacatcgt
    23161 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccaa ccctggtcac
    23221 cctgtttgag atctacgcgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    23281 cactggcaac gtgggcgcta tctggcaagg tggttcgact ggagaacgga tcgctgctta
    23341 acaattcacg atgactatta tcgcggctgg catcgctaag aagggttgcc agtttgtcac
    23401 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcataga
    23461 attgttgagc cacgctgtca ggccccccct ggacacagga accggtgcgg ggaccgttat
    23521 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    23581 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    23641 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    23701 aacacctgat tgggtttgag tccattgctg gctatccact caaggttgct cgtagctgac
    23761 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23821 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23881 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23941 acgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    24001 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    24061 ttgactctcg ataatattat ttatgctaca attgtggcta aagaatttaa gctaatgaat
    24121 tttagcgaag aataaattaa gttgtttcag tatctcgtta attaatatga atataaaagg
    24181 cgttgtgctt ttataattat aaagcacgag gagttcggtg gattatcgtt tttcttgggt
    24241 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    24301 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaattccag
    24361 tcgtaagggt gagttgtcta catgggatac catctacttg catctgatat tagcaataaa
    24421 tgctgacagt gatctcataa aaaatgatgt gttgctcgcg gaaaatatgc catcagcgaa
    24481 ttaccaattt aatacttttt ttagtaatgc tttcgagact gatgttaaaa aatatctggg
    24541 taaatcagaa gataacgaag ttgaaataaa ggcgggtaat gaaagaatct ctattggaat
    24601 acgtaatgta tcgaatggga aacttgaaag acaacagttt cttttccctc tggactatga
    24661 gaataaatta caagatcaat tagacaagca ttttacgatt gaatcgcacc cattattata
    24721 caggcatacc attggtagta aaattgcgaa tgtgattttt gaaaaattat acagccgtat
    24781 tgactttaac aaagaacaat atatatcctt tataaagggc gcgtttattc atttttatga
    24841 ttatagcagg cgatatgcaa ttagtgagaa tatcgaaaaa gacgctgtaa caaacaatat
    24901 cgcattgatg aacacttttt atgacagtga taatacgtca ggtgaggttt taaataatga
    24961 ttttacagaa gaagagccat ttgaaactgc gttagatgta gagcatacaa tagtgcttgg
    25021 atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt
    25081 tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcgctctt cgcattacca
    25141 cgatatttgt agcgtatgtg tggatatgac aaagggaacg caatgcatga ttcacttgat
    25201 ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga
    25261 tgcaagtaat ttagtcaatc ttaaaacact actcaatgat ggttttttca ttaaatatag
    25321 tcattttaat gacgtggcat taataaagca aaacataagt aatctctact tatcacacac
    25381 tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt
    25441 taaaataaca aatagcaata taataaataa tgtattcaaa aatgtttcgt ttagatctgt
    25501 taaaattaac aacgtaaata ctcattcttt aaaatttatt aattgccatt tttttaatgt
    25561 tgatatgata agagttaatt tcagcaaatg tctttttcat gactgtagta tgcatggtgt
    25621 caagataaaa ccatggttgc cagtaaaatg gacaaaagag ttaataaatg attatttgta
    25681 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgagcgccgg
    25741 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaagaataa
    25801 atattgcatt aaactattaa gcgctcagga acgggcgttt atttacgaat ttgcaagaat
    25861 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
    25921 gtctgaccaa aaagcaatcc agaggtatat acctcagtca caagatgggg aagactttag
    25981 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat
    26041 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
    26101 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
    26161 cttcccaaca tggcatatca gttcgataat gctttagaga tcgaaggctt aacaaacaag
    26221 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
    26281 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
    26341 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
    26401 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
    26461 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
    26521 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
    26581 gctctgttat tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
    26641 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
    26701 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
    26761 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
    26821 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
    26881 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
    26941 gtgaaatatt ttgccccgtt tgaaccgaca cagattcagg cgcttattcc actggcgaaa
    27001 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
    27061 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
    27121 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
    27181 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
    27241 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
    27301 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
    27361 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
    27421 ttctcgatac cttcatctag ggtgatgatc ggctgataac ctaactcttc ctgcgcacgc
    27481 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
    27541 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
    27601 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
    27661 ctgcgcagtg tgcgatgctc gccgttggtg atattgtaca cacgcccgga aggtagctta
    27721 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg tattttcata gtaggtcata
    27781 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
    27841 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
    27901 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
    27961 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
    28021 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
    28081 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
    28141 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
    28201 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
    28261 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
    28321 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
    28381 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
    28441 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
    28501 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
    28561 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
    28621 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
    28681 acgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
    28741 agctatccac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
    28801 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
    28861 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
    28921 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
    28981 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
    29041 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
    29101 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
    29161 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
    29221 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
    29281 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
    29341 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
    29401 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
    29461 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
    29521 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
    29581 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
    29641 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
    29701 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
    29761 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
    29821 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
    29881 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
    29941 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
    30001 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
    30061 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
    30121 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
    30181 tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg
    30241 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
    30301 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
    30361 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
    30421 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct tgcgggtaaa ttcccatgct
    30481 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttctag actgagtaat
    30541 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
    30601 agcgtgccgt cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgctgcg
    30661 ccaccggctt caaacagata attatgcgcg gcctgaccga caatatactc ttcgccgcgt
    30721 tcgcagtgac tgagcagagc gaccaggtta gcctgggtgc cggtcggcag aaaaatggcg
    30781 gcttctttac cggaaagctc tgcggcgtag tcctgcagag cattaacggt agggtcgtct
    30841 ccgtaaacgt cgtccccaac cggggcggcc atcattgctt cgagcatggc gcggctcggt
    30901 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
    30961 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
    31021 ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag tttgatctgc
    31081 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg accgtcgatg
    31141 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
    31201 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
    31261 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
    31321 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
    31381 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
    31441 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
    31501 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
    31561 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
    31621 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
    31681 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
    31741 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
    31801 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
    31861 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
    31921 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
    31981 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
    32041 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
    32101 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
    32161 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
    32221 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
    32281 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
    32341 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
    32401 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
    32461 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
    32521 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
    32581 agactgtcac taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct
    32641 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
    32701 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
    32761 aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat
    32821 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
    32881 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
    32941 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
    33001 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
    33061 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gagtgaagaa tttctcttta
    33121 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
    33181 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
    33241 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
    33301 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
    33361 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
    33421 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
    33481 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
    33541 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
    33601 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
    33661 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
    33721 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
    33781 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
    33841 tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
    33901 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
    33961 tgacgatcgc tttctgttca aaccaagaga gcaccagcga cagcggcaga tcgttcacac
    34021 cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
    34081 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
    34141 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
    34201 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
    34261 gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct
    34321 ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct
    34381 gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc
    34441 agccaacaat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
    34501 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
    34561 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
    34621 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
    34681 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
    34741 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
    34801 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
    34861 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat
    34921 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
    34981 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
    35041 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca
    35101 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca
    35161 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
    35221 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
    35281 tgatgccgta ttcacacgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
    35341 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
    35401 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
    35461 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
    35521 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
    35581 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    35641 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    35701 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    35761 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    35821 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    35881 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    35941 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    36001 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    36061 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    36121 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    36181 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    36241 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    36301 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    36361 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    36421 cgcagcttgt tggcgaagcg gaatgaggta acctacaatc aggggaacca gaatgattaa
    36481 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    36541 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    36601 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    36661 gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga
    36721 tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag
    36781 ggataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggggcaggg
    36841 ggcggcggcg attggccgcc ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag
    36901 ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt aaaaaaaggc
    36961 ctgacattac gccaggcctt ctgcgttaat taatcacgct tttccagcag ggtccggtaa
    37021 atcagaccac cgataatgcc gccgacaatt ggcaccaccc agaagaacca cagttgttct
    37081 aatgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg
    37141 ttagtcaccg gaatactaat taagtgaatc agggttaagg ccagaccaat agcgatcggc
    37201 gcaaaacctg ccggcgcgaa tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca
    37261 ctcaatacca gttcaactac cagcg
//
LOCUS       DAJTVE010000028        36368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000028 DAJTVE010000000
VERSION     DAJTVE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..191)
                     /locus_tag="OO439_001337"
     CDS             complement(<1..191)
                     /locus_tag="OO439_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6315742.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERS"
     gene            complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="OO439_001338"
     CDS             complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="OO439_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6315743.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="OO439_001339"
     CDS             complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="OO439_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6315744.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="OO439_001340"
     CDS             complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="OO439_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6315745.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="OO439_001341"
     CDS             complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="OO439_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6315746.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="OO439_001342"
     CDS             complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="OO439_001342"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6315747.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6806..8041
                     /gene="moeA"
                     /locus_tag="OO439_001343"
     CDS             6806..8041
                     /gene="moeA"
                     /locus_tag="OO439_001343"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6315748.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8041..8790
                     /gene="moeB"
                     /locus_tag="OO439_001344"
     CDS             8041..8790
                     /gene="moeB"
                     /locus_tag="OO439_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6315749.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8969..9631)
                     /gene="fsa"
                     /locus_tag="OO439_001345"
     CDS             complement(8969..9631)
                     /gene="fsa"
                     /locus_tag="OO439_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6315750.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9762..10661
                     /locus_tag="OO439_001346"
     CDS             9762..10661
                     /locus_tag="OO439_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6315751.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10667..13099
                     /locus_tag="OO439_001347"
     CDS             10667..13099
                     /locus_tag="OO439_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6315752.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13245..14060
                     /gene="ybiV"
                     /locus_tag="OO439_001348"
     CDS             13245..14060
                     /gene="ybiV"
                     /locus_tag="OO439_001348"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6315753.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14212..15477
                     /locus_tag="OO439_001349"
     CDS             14212..15477
                     /locus_tag="OO439_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6315754.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15444..16604)
                     /locus_tag="OO439_001350"
     CDS             complement(15444..16604)
                     /locus_tag="OO439_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6315755.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16842..18143
                     /locus_tag="OO439_001351"
     CDS             16842..18143
                     /locus_tag="OO439_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315756.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18158..20521
                     /locus_tag="OO439_001352"
     CDS             18158..20521
                     /locus_tag="OO439_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6315757.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20701..22296)
                     /locus_tag="OO439_001353"
     CDS             complement(20701..22296)
                     /locus_tag="OO439_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6315758.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22515..23435
                     /gene="ldtB"
                     /locus_tag="OO439_001354"
     CDS             22515..23435
                     /gene="ldtB"
                     /locus_tag="OO439_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6315759.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23494..24612)
                     /locus_tag="OO439_001355"
     CDS             complement(23494..24612)
                     /locus_tag="OO439_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6315760.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24609..25076)
                     /gene="mntR"
                     /locus_tag="OO439_001356"
     CDS             complement(24609..25076)
                     /gene="mntR"
                     /locus_tag="OO439_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6315761.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25262..25390
                     /gene="mntS"
                     /locus_tag="OO439_001357"
     CDS             25262..25390
                     /gene="mntS"
                     /locus_tag="OO439_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6315762.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25662..27245
                     /gene="opgE"
                     /locus_tag="OO439_001358"
     CDS             25662..27245
                     /gene="opgE"
                     /locus_tag="OO439_001358"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6315763.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27294..27809)
                     /gene="ompX"
                     /locus_tag="OO439_001359"
     CDS             complement(27294..27809)
                     /gene="ompX"
                     /locus_tag="OO439_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6315764.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27862..27927)
                     /locus_tag="OO439_001360"
     CDS             complement(27862..27927)
                     /locus_tag="OO439_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315765.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28162..29049
                     /gene="rhtA"
                     /locus_tag="OO439_001361"
     CDS             28162..29049
                     /gene="rhtA"
                     /locus_tag="OO439_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6315766.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29348..29851
                     /gene="dps"
                     /locus_tag="OO439_001362"
                     /gene_synonym="pexB"
     CDS             29348..29851
                     /gene="dps"
                     /locus_tag="OO439_001362"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6315767.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30255..31001
                     /gene="glnH"
                     /locus_tag="OO439_001363"
     CDS             30255..31001
                     /gene="glnH"
                     /locus_tag="OO439_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6315768.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31140..31799
                     /gene="glnP"
                     /locus_tag="OO439_001364"
     CDS             31140..31799
                     /gene="glnP"
                     /locus_tag="OO439_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6315769.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31796..32518
                     /gene="glnQ"
                     /locus_tag="OO439_001365"
     CDS             31796..32518
                     /gene="glnQ"
                     /locus_tag="OO439_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6315770.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32635..34860
                     /gene="ybiO"
                     /locus_tag="OO439_001366"
     CDS             32635..34860
                     /gene="ybiO"
                     /locus_tag="OO439_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6315771.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(34857..35783)
                     /gene="rlmF"
                     /locus_tag="OO439_001367"
     CDS             complement(34857..35783)
                     /gene="rlmF"
                     /locus_tag="OO439_001367"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6315772.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36059..36319
                     /locus_tag="OO439_001368"
     CDS             36059..36319
                     /locus_tag="OO439_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6315773.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         8772 a         9841 c         9161 g         8594 t
ORIGIN      
        1 gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta
       61 tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatgatgaa aaaaagacca
      121 ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat cttttcgtat
      181 aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc cgctgcaaaa
      241 atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt
      301 tacaacactt gcttagggca ataatttcct gtgaggtgat taccctttca agcaatattc
      361 aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa
      421 ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac atgcggcgcg
      481 ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt cggcggctgc
      541 gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc aatgcgcatg
      601 gtgaaaaaca ccacgataga cgagacggtc ccaggcaaga tatgacgcaa taaaatggtc
      661 atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt caacaccagc
      721 gtgttgccgc gcaccagtcg ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc
      781 acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa aatacccggg
      841 aaggcaaaca gcacatcgca aatgcgcatg atcagccgat cccaccagcc ttcataatat
      901 ccagccaaca agcccagcaa cgtcccgatc gccgcaccga taaacacggc aaacacgccc
      961 gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg ccccagtgaa
     1021 tcgacgccaa accagtgctg caaagaaggt ccgttattca gattgtcata atcaaaataa
     1081 ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat caataaaata
     1141 acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc
     1201 cacggtgtac gcacctggtc aggtttgacc agtggcatgg cgtttaacac cgcctgacgt
     1261 cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg gcggcgtaaa
     1321 gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga atttccgcct
     1381 gaatcaccgg gtaatcgcgc atttctacgg agtcaacgag taagcgtcca agtccgggcc
     1441 agttgaaaac tttctccacc acgatagaac caccgagcaa aaagccaaac tgtaagccca
     1501 tcatggtcac taccgggatc atcgcgttac gtagcccgtg tttgaggaca acccaggttt
     1561 cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaaca tcgacaaacg
     1621 acgcgcgggt aaagcgcgcc atcacggctg ccactgccgc gccgagcgtc agggagggta
     1681 aaatgtagtg ctgccaactg tctgctccca cggtaggcag ccagcccagt tcaacggaga
     1741 atacctgaat taaaagcatc cccagcgcaa atgccggaaa cgagatcccc gacaccgcaa
     1801 tggtcatact caatcgatcc ggccaacgat tacgccagac ggcggcgata attcccgccg
     1861 ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc gttggcataa
     1921 agcggctggc aatctcatcg gcaaccggac gacgcgacac catcgacagg ccaaaatccc
     1981 cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga tccaacccca
     2041 gctgctgacg caccagttct ataacctgcg catcagcttc gagcccggca atcaatcgcg
     2101 ccggatcgcc gggcagcata tgcacaaata aaaacaccag caccgagacg ataaacagcg
     2161 tcggaatcaa ccccagtaag cgtttgataa cgtaattaag cattccactc cctgcgttgc
     2221 ttattgcaaa tccgcgtctt caaagctgaa gccggtgtct ggcatgatcc aaaaaccggt
     2281 caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc acggcgactc
     2341 ttgccagatg atatcctgcg ccgccttata caagcgggtc ttttccgccg gatcattagt
     2401 tttcagtgcc tgagccagga agtcgtccac ctgtttattg ctgtaaaatg cggtattaaa
     2461 cagcgtcggt ggccagtttt gcgaggcgaa cagtggcgat agcgcccagt ccgcttcgcc
     2521 ggttgaagcc gaccagccag tgtagaacat ccgcacgccg ctctcttttt gccctttacc
     2581 ttcaacttct gccgcccgct gtccggcatc catcgcggtc acctgggctt taatcccgac
     2641 ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt ggttatgtga
     2701 cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta attcgcgcgc
     2761 tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac ttggcggtac
     2821 cacaccagta gctggcgttg catagcccgc aaaggcaact ttcaccagcg ccggacggtt
     2881 aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt gcgtcacgtt
     2941 catactgata taacgctgca taattgacgg actggccatc aactcgatat ttttgttttt
     3001 ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt caccggtttg
     3061 cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc tgtccagttt
     3121 gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt
     3181 ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct ccttgccata
     3241 tttttccagc gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa
     3301 cgctgagaac ggctgtttga gggtaatctt taccgttgtc ggatcgatcg cctccgtttt
     3361 ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc tggcccggtc
     3421 cagattcgct tttaccgcca ctgcgttgaa atcggtgcca tcctggaatt taatgccttc
     3481 ccgcaatttc acggtgtaag taatgccgtc atcggaaacg gtataactct ccgccagcac
     3541 gtttttcagt ttcatctctt tatccagacc gaacagcccc tggtaaaacg atttcgctac
     3601 ggcctgagat aacgtatcat tcgcgtcata cggatcgagc gtggtgaaat tcgatcctac
     3661 cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc
     3721 cagcgccact aacccactac ggtgtacagc tcttgccatt ttattctccg cctgaatgtt
     3781 atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc
     3841 attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgctg ggaagatcgt
     3901 ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga actgccgcca
     3961 gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt ggaccaattt
     4021 caacaatttg cccgagatac atcaccgcca cacgatgact aatccgctct accacggcca
     4081 tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt
     4141 tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg gcaatgatca
     4201 cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa
     4261 actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc agcaaccacg
     4321 caacgcgtgc agccgcgtct ttacctggca ataaaccgtg tacacgcagc ggttcgataa
     4381 tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga aaaataaact
     4441 gaatatcccg gcgcaatgcc tgaagtttgc cgggtgacaa ggtatcgatt ctctgaccgt
     4501 taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg
     4561 atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag agatcaaaac
     4621 tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag
     4681 ggaaacgggt gacaagatta cgcactcgta aaacaggttc gccatccacc accgttttct
     4741 gctcgatggg gggggcctgt ttcgctggat gttcaagcga tatcaacggg aaacgtcggg
     4801 gataatccaa ccctttcatc gcaccaagtt gcggaacagc agctaacagc gcacgggtgt
     4861 aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc gcctcgccct
     4921 gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg tgagtgataa
     4981 agataacgcc catcgacatc tctttttgca atactttgat taattgcagg atctgcgcct
     5041 gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc gggcggcatg
     5101 acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga tgcggataac
     5161 gtgaaagaat ggtttgtgcc tcaggaatgc gtacctgatc cagcatccgc ttcgcctcga
     5221 ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca atctgttcac
     5281 ccacagtaaa taccgggttc agcgatgtca tcggctcctg aaatatcatc gccatatccg
     5341 caccgcgaac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc acttcgcgac
     5401 tgcgccgctg caacagcatt ttatcgcact gtactaaacc gcccgcctgt tccaacaggc
     5461 gcatcaatgc caacgcagtc actgacttac cggagccgga ttcgccaaca attgccagcg
     5521 tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc tgctggtcct
     5581 gcataaaggc aatattcaga ttttcaaccg ccagcacatt accggcatca agttcatcac
     5641 tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt atcgcctgcg
     5701 tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt cccttcatgg
     5761 tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat tactacccgc
     5821 tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat gtcatatgcc
     5881 gccagcgcgc ggatgaagac ttcgcccgtg ccgatacaag aaaccgccac actggcgtta
     5941 ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg taatttattg
     6001 gtcattccgc ctgtggacgt ggctgccgcc aaattgccgt ctaaatccaa cgccacggcc
     6061 cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc gaggacggtt
     6121 gccccttcct tgcgcgctgc cagtagttgt tcataacgca aaggcgtgga gaaaatctcc
     6181 ggcgagacgc gctccatgcc acgagcaaac gcaaaatttt ctgccccttc gccaatcatc
     6241 atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt acgcagatga
     6301 ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca ggcgtccagt
     6361 tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag tggacactct
     6421 tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc ttccagcatt
     6481 ttctgcccgg tttcaacaat ggcagacaac gcctcaatgt agcgtaattc ctgttgcaga
     6541 ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac tgctttgccc
     6601 atagtattcg tccattaagg gtggctcgcc acttttgcta taaatatcag aatttttatg
     6661 aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag gcaggagcca
     6721 acgctacgcc cgaggtggca tacagtctgt ggcgtttttc tgacataata ggcaaattcg
     6781 attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt cgctcgacac
     6841 cgcgcttaat gagatgcttt ctcgcgtcac cccactgacc gcccaggaaa cgctgccact
     6901 ggtacagtgt tttggtcgta ttctggcgag cgatgtcgtt tcgccgcttg atgttccggg
     6961 gtttgataac tccgcaatgg acggctacgc ggtgcgttta gccgatattg cctccgggca
     7021 accgctgccc gttgccggta aatcctttgc cggtcagccg tatcatggtg aatggcctgc
     7081 gggcacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg aagcggtggt
     7141 gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag
     7201 cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg ttttcccggc
     7261 gggaactcgc ctgactaccg cagagctgcc agtaattgct tcactgggga ttgccgaagt
     7321 tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac tccagttgcc
     7381 cggtcagccg ctgggcgacg gccaaatcta cgacaccaac cgtctcgccg tacacctgat
     7441 gttagaacag ctgggttgcg aggtaatcaa cttagggatt atccgcgacg atccccatgc
     7501 cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtagtgatca gttccggcgg
     7561 tgtttcagtg ggtgaagcgg attacaccaa aacgattctt gaagagctgg gggagatcgc
     7621 cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac tcagcaatag
     7681 ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct atcaactggt
     7741 acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg cgcgccagcg
     7801 cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc agcgcggcgt
     7861 gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc agggttcaca
     7921 tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa
     7981 tgtggacgtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg gaggcctgta
     8041 atggcggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt
     8101 tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctgatcgt cggcctcggt
     8161 ggactcggct gcgcagcttc gctgtatctg gcaagcgccg gtgtcggcaa cctgacgctg
     8221 ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc agacactgca cagtgatgcc
     8281 acggtcgggc aaccgaaggt ggaatccgcc cgtgacgccc tgacgcggat caacccacat
     8341 atcgcgatta cgccagtcaa tgcactgcta gatgacgcag aacttgcagc attgattgct
     8401 gaacacgatc tggtgctcga ctgtacggat aacgttgcgg tacgtaatca actgaacgca
     8461 ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat ggaagggcaa
     8521 atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag ccgtttgttt
     8581 ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat cggcgtaatt
     8641 ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa accgaccagc
     8701 gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa actgatgcgc
     8761 aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggcctgat aagacgcgac
     8821 aagcgtcgca tcaggcattt gctccagacg ccagatgcgg cgtgaacgcc ttatctggcc
     8881 tacggttcgg gcacagattt ccaagcgcgg caggcatcgg tgcactccgc cacgtcatca
     8941 gcaatttatg aggatgcaga acgtccggtt aaatcgacgt tctgccaaac gctccctgcc
     9001 agtcctgctc aaacttcgcc acagcggctt ccaccgccgg atagctaatc atctgttgtg
     9061 ccacatccag tggcagagta attgattcac atcctgccag taagcagtcc agcgcctgac
     9121 gcggggtttt gaaactcgct gccagcactt tcgcctgcgg cgcatgcatt ttcaataact
     9181 ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg agcatcaata cgattaacgt
     9241 aaggcgcaac atattccgca cctgccagcg ccgacagcaa cccttgtgct gcgccatata
     9301 ccgcggttcc cagcgttgga atcccttccg cttttaacat cttaatagct gccagcccct
     9361 cggcggtcac cggaactttc accacgatat ccgcaataat agaacgcaat ttacgtgcgt
     9421 cattcaccat cccttcagca gtggtagcca ttacctgggc aaacagacgc ccctgaccgc
     9481 ccatcgcttc atgaagttgc ggaagcacaa catccagcgg ttttttaccc gcggcgataa
     9541 tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa
     9601 caacgtctga agtatccaga tacagttcca taaccatcct caaaatgtac tttttgtctg
     9661 aatgtagact atcacgggaa aactctctga acagttgacg cacatcaatc taactttcat
     9721 tcgaaagtat tttaatgttt gaacgaaaca gagaggcgac tatgattttc aatattcagc
     9781 gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc
     9841 tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat ctgctgtatg
     9901 acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg
     9961 agcgcgcgct gaatggtttg cttattcatc gggaaaagtt aaccccggag catctgacgg
    10021 cgttaaccga ctgctgtccg acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg
    10081 aggagatcat gacgaccgtt ctgcgcgata aaccgtttta cgatcgcagc ggcggcggtt
    10141 taacgctttc gggtggtgag ccctttatgc agccggaaat ggcgatggcg ctactgcaag
    10201 ccagccacga ggcaggcatt catactgcgg tagaaacctg tctgcatgtg ccgtggaaat
    10261 atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg
    10321 acgcgccgtt taaacagtgg accgacggta acgccgccag agtgctggat aacctgaaaa
    10381 aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag ggctttaatg
    10441 ccgacgaaac ctctgtaaaa gctattaccg attttgccgc cgacgagctg cacgttggcg
    10501 aaattcattt cctgccctac cacacgctag gcatcaacaa atatcactta cttaatctgc
    10561 cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt gcccagcagt
    10621 atgcctgcca gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa
    10681 actggacacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa
    10741 accgccagtc tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga
    10801 taagccgatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat
    10861 ctggatcaaa cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc
    10921 gatcttcccg gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg
    10981 tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc
    11041 gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg atgagcaaaa
    11101 aggtctgctg gcgaccggaa tcattaaagc ggaaggcaat atgacctccg gcgatgcgca
    11161 cctggcggtg aatttcccgc tgctgctgga aaaaggtctt gatggtctgc gcgagaaagt
    11221 agcggaacgt cgctcgcgca tcaatctgac ggtactggaa gatttgcacg gcgaacagtt
    11281 cctgaaagcg attgatatcg tgctggtggc agtcagcgaa cacattgaac gttttgcagc
    11341 cctggcgcgt gaaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc
    11401 gatagcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct
    11461 gcaactgtgt tacttcatcc aattgatatt gcagatcgaa tctaacggtc actcggtatc
    11521 gtttggtcgt atggaccagt atctctaccc gtactatcgc cgcgacgttg aactcaacca
    11581 gacactggat cgcgaacacg ccatcgaatt gctgcatagc tgctggctga aactgctgga
    11641 agtgaacaag atccgctccg gctcacactc aaaagcctct gcgggaagtc ctctgtatca
    11701 gaacgtcacc attggcggac aaactctggt tgatggtcaa ccaatggacg cggtgaatcc
    11761 actctcttac gctatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag
    11821 cgtgcgttac catgcaggaa tgagtaatga tttcctcgac gcctgcgtac aggtgatccg
    11881 ttgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa
    11941 actcggtatt gaaccgcagg acgcttacga ctacgcagcg attggttgta tcgaaaccgc
    12001 cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt
    12061 gatgctggcg gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca
    12121 agaaaaagcg ttgtcggcag gcaactttaa caatttcgat gaagtaatgg acgcgtggga
    12181 tacgcaaatc cgttattaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat
    12241 gctggaagag aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg
    12301 agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggttgg
    12361 cattgccaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg aacagggcgc
    12421 gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga
    12481 gcaactgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt
    12541 cgatacgctg ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa
    12601 cccgcgctac ggtcgtggtc cggttggcgg caactattac gcgggtacct cgtctatctc
    12661 cgctaacgta ccgtttggcg cgcaaactat ggcaacaccg gacgggcgta aagcccacac
    12721 cccgctggca gaaggcgcaa gcccggcctc cggtactgac catcttggcc ctactgcggt
    12781 cattggctca gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca
    12841 gaaactgaat ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct
    12901 gcgtaccttc tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga
    12961 aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc
    13021 gggctattcc gccttcttca ccgcgctctc tccagacgca caggacgata tcatcgcccg
    13081 tactgaacat atgctgtaat ctcctcggcc cggcggcgtc gtcgccgggc taaatcattc
    13141 acatcatcaa tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg
    13201 ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta
    13261 tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt
    13321 ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta
    13381 atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg
    13441 cggaaaacgg cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc
    13501 gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg
    13561 cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga
    13621 tggcaaaaca ctaccatcgc ctgaaagctg taaaagatta tcaggagatt gacgacgtac
    13681 tgttcaagtt ttcgctcaac ctgccagatg aacaaatccc gttagtgatc gacaaactgc
    13741 acatagcgct cgatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga
    13801 ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt
    13861 caccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg
    13921 cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg
    13981 ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcaggcggtg ctggataaca
    14041 catccccttt taacagctga cctctctacc ggagccgtgc gctccggttt tcccttcttt
    14101 ttacatcctg tacatcacat caaatcatta aacttgcatt aacttattta taacttatat
    14161 tgtcgcatgc aaatcagaat ggtttcgaat gaaagttaat gagaggtggt tatggcttct
    14221 acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac
    14281 gcgctgcggg cgcagcttgg cgacgtccag cagatcttta atcagctaag cgatgacatt
    14341 gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg
    14401 ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa
    14461 cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat
    14521 cagtcgatgc tggactatct ggaccgcaac cgctttgacg aggtctacaa aggccccggc
    14581 gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg
    14641 caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat
    14701 cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc
    14761 taccctgacc gtatccgccg ccgtccgccc ggaacgacct ccaaaggtct tggagcgcat
    14821 accgactccg gggcgctgga acgctggctg cttccagcgt atcagcgcgt tttcgccaac
    14881 gtctttaatg gcaatctggc gcaatatgat ccctggcatg cggcacatcg tacggaagtt
    14941 gaagagtaca cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccaaggctgg
    15001 acagcgctct ctaatatgct gcctggtcag gggctgctgc acgttgtgcc cattcctgaa
    15061 gccatggcgt acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc
    15121 ggcgtagcgc ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag
    15181 gcgttaacca gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc
    15241 atccattccg ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct
    15301 gcggcaccaa tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa
    15361 aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc
    15421 tttacgcttg ccgacctcaa cattcacggt aagcgagcgt tgggaatcga cagctgagtg
    15481 tcgtacagcc cttcccgttc cacggcggtg ttcccttttc ctgggcgaac ccgccgcact
    15541 gactcccgca ccgccaatcg tccgtgcagc agcagcgagc ccaccggagc atccgggcga
    15601 atcagccgct gttgcaacat ttgcaccgct tcattgccta gctcatcgcg tggcacatgc
    15661 acagccgtca ggggaacatc ctcgattgcc gcaaggttaa agccgtcgat actcatcacc
    15721 gagacatcct gtggtacgcg cagaccacga cgatgtagcg catttaccgt tcccgccgcc
    15781 ataaaatccc cgccgaccag aaacgccgtc ggcaacgtct tgccattggc ggcatccagc
    15841 cacgctccga ccttttcttc cgcttcttta gcgctaaaac tgctgatatt cagcaaatga
    15901 cggtcatggt gaaaggccag attatgctga cgccatgatt ctttaatccc tgcaaaccgc
    15961 tgctccatag tgtagcggcg caggcacatc acattcatca cctcccggtg acccatttca
    16021 aacagatagc gggcggcgaa cgcgccaatt aaccgatgat ccggcgcaat cgccgggagg
    16081 cgcattcgtt catcacggca gttgatcagc acacaaggct tagcgagatc ggcggccaga
    16141 tcatggatgt gcggatcgtc aatccccagc aggatcgctg cctgcgtatc tgcttcattc
    16201 atccgcgaga gaaagagcgc gggatcgctg tcaaactcct ccagcgcgca gtaacgcagc
    16261 cgcacttcat gcggcgataa cgctttgctg atgctctgga tcacacggta ataaaagata
    16321 tcagatcgtt ggtcaaaagc gcgttgcggc gcgaaaataa tcaggttgtt cagcaacata
    16381 cgacctgctg ccatgccatc catcacgccc aactcacgcg cgcaggccag cacctgttcg
    16441 cgagcgcgtg cactggtatt cgctttgcca gccagcaccc gcgaaaccgt actcggcgat
    16501 agcccggtac gtgcggcgat atcggcgatt ttcagcttct tgtccatttt gtgatctgcc
    16561 tctaattcgg aaatgacaga attttcatga gcaaaaaagc ccattctgtc tggcattaaa
    16621 tccatttcag taatgtatat cagtcactca tggaaaaacg tgcacaaaac ccgctttctt
    16681 tcgcttttag ggtctcttac tgtgaattgg tagaacaact tccatacaaa aataatggaa
    16741 aaacgaacaa taaagacttt atttatcaaa aagttaaaag tggtgtaaca actccattct
    16801 gacgttgccg cacaaaccct ataccattct gtggagtgca tatgagtcag ggtatcaata
    16861 acgatatgac tgccagtaaa ccgcgccata tcgtcaaaaa tctgcgttgg tggatactgg
    16921 tccttttttt gctcggtgtt actgtcaact acatcacgcg taactcgctg ggtattctcg
    16981 cccccgaact gaaaagcagc ctagggatta ccaccgaaca atattcctgg attgtaggtg
    17041 ctttccagct cgcctatacc ctattccaac cgttgtgcgg atggctgatt gacgtcattg
    17101 gcctcaaact gggctttatg atctgcgcgg gactgtgggc actggcctgt ctgatgcatg
    17161 cgggggcggg aaactgggga catctggcgc tgttgcgctt ctttatgggc ggtgcggaag
    17221 ccgcggcaac cccagcaaac gccaaaacca tcggtgaatg gtttccaaaa tcagaacgcc
    17281 cgatcgccgc aggctgggca ggcgtcggtt tttccatcgg tgccatgctg gccccaccga
    17341 ttatctattt tgcccacgct tcattcggct ggcagggtgc atttatgttc actggcatcc
    17401 tggcgatgct gtgggtggtg ctgtggtggg cgttctatca cagcccggat aagcatccta
    17461 acctcagcaa agccgagctg gactttattc gccaggataa cgaagcgccc ccggtcaagt
    17521 taccctttct gaccgcactg aaaaccgtgg gcaaaaataa acgcttttac ggtatcgcta
    17581 tcccggcctt tatggcagaa ccggcgtggg cggtgatgag tttctgggtg cctctatacc
    17641 tgtccaaaac ctatggcatg gacctgaagc agatcgcatt attcgcctgg ttgccgttcc
    17701 tcgccgctga tcttggcagt attgccagcg gctatctgac caaactttac acccgcctgt
    17761 ttgggctgag tcgtgtgaac tccgttgtcg catcatcggt cagtggcgca ttcttgatgg
    17821 tttcgctggc ggtgatggcc tttaccaata acccgtatat cgccatcgtc cttatctcca
    17881 tcggcggctt cggtcaccag atcatctcct gcatgctgag cgcactggtg gtggaatcct
    17941 tcgacaaagg tcagatggcc actgttaacg gcatgcgcgg ttctgccgcg tggatcgcaa
    18001 gtttcctgtt ctccctgatt atcggggtta ccgctgacaa aatcggcttt aaccccctgt
    18061 ttatcgccat gggcttcttt gacctgattg gcgctttctt cctggtcacc tttattgctg
    18121 aacgtcgcgc aaaacgcgcc tgagagtgga tgtaaacatg aaaacgctga aaaactggac
    18181 cctgcacaaa cagtctgccc accacgttga actgctggtc gatggccagc atatcctgtg
    18241 cctgtacgtg ctggaagaaa atctgttccg cgtgctgtta aaacgcaaag gcgagctggc
    18301 gctagatcgc acctggagca ttgctccggc gcaggatgtg ccatgggaag ggcgcagccg
    18361 cgaagatctc agcggcttta gcctgcccgc gtggacagtg atccagcacg acgacaccgt
    18421 ggttatcgcc accgagtcac tgcgtgcaac cgttcaccag ccgctgtggc tggagtggca
    18481 ctatcgtaac aacgaaggcg aatggctgcc gttggtgaat gaccgcccga ccagcgctta
    18541 tctgctgaat gcccacggcg atggcgtggc acactatcag agccgtcgta aagatgaacg
    18601 cttttatggc ctcggagata aagccggcga tctgcaacgc aacggtaaac gttacgagat
    18661 gcgtaacctc gacgcgatgg gctacaacgc cgttagtact gacccgctgt acaaacacat
    18721 tccattcacc atcacccgcc gcgatgacat tagcttcggc ctgttttatg acaacctcag
    18781 cagctgctgg ctggatctgg gcaacgagat cgacaactac cacaccgcct atcgccgctg
    18841 gcaggcggaa gcgggtgata tcgactacta cctgtttacc ggcgaacgcg tacttgatat
    18901 caccaaagcc ttcgtacgcc tgaccggaaa aaccctgttc ggaccgaaat ggagcctggg
    18961 ctacagcggt tcaactatgc actacaccga tgcgccggac gcacaaaatc agttgatgaa
    19021 ctttatccgc ctgtgtgaag aacatgcaat tccctgcgat tcgttccagc tctcttccgg
    19081 ttatacctca attaacggca aacgctatgt gttcaactgg aactacgaca aagtgccgca
    19141 gccgaaagtg atgagccagg catttcacaa tgctggtttg cacctggccg ccaatatcaa
    19201 gccgtgcctg ttgcaggatc acccacgcta taacgaagtg gcagaaagag gactgttcat
    19261 ccgtgattca gaaacagatg aaccagaacg ttccagcttc tgggatgacg aaggttctaa
    19321 cctcgagttt actaatccgc agaccatcca gtggtggcag gaaggcgtga ccacacagct
    19381 tctggagatg ggtatcgact ccacctggaa cgacaacaac gaattcgaag tatgggacgg
    19441 tgaagcacgc tgtcacggct ttggtcagac gatcgcgatc aaacatattc gcccagtgat
    19501 gccactgctg atgatgcgag cctcgatgga agcccagcag cgtttcgctc cggaaaaacg
    19561 tccgtacttg atctcccgct ccggctgcgc gggaatgcag cgctacgtgc agacctggag
    19621 tggcgataac cgcactagct gggacactct gcgctacaac acccgcatgg gtctggggat
    19681 gagcctctcc gggctgtata acctcggtca cgacgtcggc ggtttctccg gtgataagcc
    19741 ggacccggaa ctgtttgtac gctgggtaca aaacggcgtg atgcatccgc gctttacgat
    19801 tcactcatgg aacgatgacc ataccgtaaa cgaaccgtgg atgtatccgg gcgtcactcc
    19861 ggcgatccgc agcgctattg agttgcgcta tcgcctgctg ccctactttt ataccctgat
    19921 gtggcaggca tacgccgatg atgaaccaat gctacgtccg actttccttg accacgagca
    19981 cgacgttcag acttttgaag agtgtgatga cttcctgctt ggtcgcgata ttttggttgc
    20041 cagcgtcgta gagcaaggcg agcgccagcg ccgtgtatgg ctgccagata acgaaactgg
    20101 ctggtacgat ttctacaacg gcgaatggtt cagtggcggt cagtggatca ccctcgacgc
    20161 accgctggag aaactgccgc tgatggttcg tgccggggct ggtctaccgc tcagcaaacg
    20221 catcacctat gtgagcgcag aacaagacga tacccgcgaa ctgaaactgt tcccgctgaa
    20281 aggtgtgggc acaacgtccg gcctgctgtt tgaagacgat ggcgaaagct ggggctacca
    20341 aactggtaat gcgctttggg tggaatggga aatggtttgc gatggggcga ccatcaacct
    20401 gaaagtcaac gcgcgcgggg attatcgtcc tgcatggagt gcactgaaag tgtcattacc
    20461 attggaagaa aaacgcacgc tgctggtcaa tggtgttgaa gggagtgaat ggatgcgtta
    20521 aatcattacc ggggccggga ggcgactgca ccttacccgg tccagccatc gaatccttta
    20581 cctcagtatt tcacgctgca ggaaggcagc aaacctgtaa accccgggag cttaaataag
    20641 taagtgaccg gggtgaacag gtgaagccaa tgcatctgcc gcatgaaaga cggcggggaa
    20701 ttaagcgtcg atccctttac tacgcaggta atcttcgtaa ttaccgctaa agtcgatcgc
    20761 gcgttccggg gtgatttcca gaatgcgggt cgccagggag cttacgaact cacggtcgtg
    20821 tgaaacaaag atcagcgtgc cctgatacag ttccagcgcc atgttcagtg actcgatgga
    20881 ttccatatcc aggtggttgg tcggttcgtc catgatcaga atgttcggct tctgcatcat
    20941 taacttacca aacagcatcc gccctttttc cccaccggaa agcactttag ctggcttttt
    21001 gatgtcgtcc tggctgaaca gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc
    21061 gccttcctgc ttccactgac tcatccattc gaacacggtc agatcatttt caaactcata
    21121 ttcgtggtcc tgggcgtagt aaccaatgcg cgcgttttca gaccatttta cggtgccgct
    21181 gtccggttgc agatcgccca ccagcgtttt cagcagcgtt gatttaccga cgccgttggt
    21241 acccagtacc gcaagttttt caccgacctc cagcagcagg ttgagatttt taaacagtgg
    21301 accgttatca aaccctttgg tcagaccttc cacttccagc gcgttacgga acagtttctt
    21361 atcctgttca aaacggatga acgggttctg acggctggag gctttcacct cttccagttt
    21421 gattttatca atctggcgcg cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct
    21481 aaagcggcta acgaaagatt gcaactcagc aatctgcgct ttcttcttgg cgttatcggc
    21541 cagcagacgt tcacgcgcct gggtcgccgc cgtcatgtac tcatcgtagt tacccggata
    21601 aacgcgcagc tcgccgtaat ccagatccgc catgtgggta cagaccatgt taaggaagtg
    21661 acggtcgtgc gagatgatga tcatggtgct gtcacgctcg ttcagcacct gttccagcca
    21721 gcgaatggtg tcgatgtcga ggttgttggt cggttcgtcg agcaggagaa tctccggatc
    21781 agcaaacagc gcctgcgcca gaagcacacg cagtttccag ccaggagcaa cttcactcat
    21841 cgggccgtag tgctgttcca ctggaattcc cacgccaagc aacagttcac cggcgcgagc
    21901 ttccgcagag taaccgtcca tttcgccgta tttaacttcc agatcggcca ctttatagcc
    21961 gtcttcctca ctcatttccg gcaaagcata gatgcggtca cgctcctgct tcacttccca
    22021 caactcttta tgccccatga tcaccgtatc cagcacagtg aactcttcaa aggcaaactg
    22081 atcctgacgc agcttaccaa tgcgctcgtt gggatcgagg gaaacgttac ccaaagtcgg
    22141 ctctaagtcg ccgccgagga tcttcataaa ggtggattta ccactaccgt tcgcgccaat
    22201 caggccgtaa cggttgccgc cgccgaattt gacggaaatg ttttcaaaca acggcttact
    22261 gccgaactgc atggtgacgt tactggaaac taacacaggc gtatcctgaa aagagatatg
    22321 acaaaccgcg cattatgcca taagtctgaa agaagatcct gctcctcgct taaggtctac
    22381 actcaagcca aaggcaaaaa atgtgatttc gtacacatct gatttcactg tgagctggaa
    22441 tgaacttata atgcgcttcc aatactctaa tattctcaac ccaatggcct gccaggcaca
    22501 aaatctcgct taacatgaat atgaaattga aaacattatt cgcagcggcc ttcgctgttg
    22561 tcggcttttg cagtaccgcc tctgcggtaa cttatcctct gccaaccgac gggagtcgcc
    22621 tggttggtca gaatcaggtg atcaccattc ctgaaggtaa cactcagccg ctggagtatt
    22681 ttgccgcgga gtaccagatg gggctttcca atatgatgga agcgaacccg ggtgtggata
    22741 ccttcctgcc gaaaggcggt actgtactga acattccgca gcagctgatc ctgccggata
    22801 ccgttcatga aggcatcgtc attaacagtg cagagatgcg tctgtattac tatccgaaag
    22861 ggaccaacac cgttatcgtg ctgccgatcg gcattggtca gttaggcaaa gatacgccta
    22921 tcaactggac caccaaagtt gagcgtaaga aagcaggccc gacctggacg ccgaccgcca
    22981 aaatgcacgc agagtaccgc gctgcgggcg aaccgcttcc ggctgtcgtt ccggcaggtc
    23041 cggataaccc gatggggctg tatgcactct acatcggtcg cctgtacgct atccatggca
    23101 ccaacgccaa cttcggtatc ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg
    23161 aagacatcaa attcctgttc gagaaagtac cggtcggtac ccgcgtacag tttattgatg
    23221 agccggtaaa agcgaccacc gagccagacg gcagccgtta tattgaagtc cataacccgc
    23281 tgtctaccac cgaagcccag tttgaaggtc aggaaattgt gccaattacc ctgacgaaga
    23341 gcgtgcaaac agtgaccggt cagccagatg ttgaccaggt tgttcttgat gaagcgatta
    23401 aaaaccgctc cgggatgccg gttcgtctga attaatcttc aaaacttaaa gcaaaaggcg
    23461 gactcataat ccgccttttt tatttgccag accctagttg gccgggagta taactaacaa
    23521 aacatatccc accaacgccg cccacaacag catcggtatt gaatagagat ggaaacgcca
    23581 ccagatgcgg cgatcgttcg ccatacgtag cgcaatcaaa tttgccagcg atccgggtaa
    23641 taacccaaag ccacctacgt ttaccgccca taccagtaat aaagacggcg gcacatagtt
    23701 cagcaacaat atggtactcg gcacgttact gatcacctgc gataaaccga ttgccgttaa
    23761 ccataacccg ggttcagata gatgactcac gttacccaac acgccttgca acgctggaag
    23821 ctgggtcagt aaatggacgt cgataaacat cgccataaac accagcagca gcgtccagtc
    23881 cacactgagc accacgcgac gtgccagcag cgcaaagcct gccgccacaa tcaccagtcc
    23941 ccacagctct tgtttgaact ccagcgccgt cagaaagacg atatacagcc ccagacaact
    24001 ccacaccagc cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcgcctt
    24061 tccagggaaa caacaccagc acaggagcag gagcgtcagc atcattgcgc cagccagcgg
    24121 tgccatttgg gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt
    24181 ttgcgggttg ccaattggcg tcagtagcga accagcgttg actgccagcg cctcaaaaat
    24241 aatcagccga ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac
    24301 aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat
    24361 aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc
    24421 aaaataaccg cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat
    24481 ggtgtgccag tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa
    24541 tccgatacca acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa
    24601 aggcaggctc atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct
    24661 tcactaacat ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc
    24721 aacaccaaca agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc
    24781 tctccttctg ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt
    24841 gccagccgct taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg
    24901 tccacctgac gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca
    24961 tcaataagct cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc
    25021 acgttcacta actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg
    25081 cttcctcatt atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag
    25141 agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct acataatata gcctgtgcta
    25201 tatctgtatg taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct
    25261 tatgaatgag ttcaagaagt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt
    25321 aatgctgctc tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc
    25381 cgataaatag cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcataacgcg
    25441 ttttggtctg aaaaacccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt
    25501 tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc ttgcagccct acgtatattg
    25561 cggctcgcgg atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg
    25621 acgccagggt aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag
    25681 aatcgcttgt tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt
    25741 cgctattaat taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta
    25801 cggctatttt gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg
    25861 tcagttcgct ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt
    25921 tcaatacatt gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga
    25981 tttttccgtg gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa
    26041 tcaggcgaaa aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg
    26101 tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat
    26161 ttaaactgaa agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata
    26221 ataacgaggt gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct
    26281 ggacggtcac tgccgttaag ccgaagtatc agacctatgt ggtggtgatc ggcgaaagcg
    26341 cgcgtcgcga tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca
    26401 gcgttaacgg tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcac
    26461 ttggcttaac gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca
    26521 ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg
    26581 aatacgatac tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag
    26641 aaggtaattt cgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc
    26701 aagtgctggc gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc
    26761 atccgcaggc ctgcgacagg acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa
    26821 cgtcgtgcta tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc
    26881 agttacgcaa cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct
    26941 ttaaagagcg cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt
    27001 tccaggtgcc ttttatggtc atttccagcg acgataaagc gcatcgggtg attaaagccc
    27061 gccgctcagc caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg
    27121 aaataaatat caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca
    27181 acttccagtt acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac
    27241 cttaaaacaa aaaatccgcc ccgagaggcg gattttttat atcaccaaag tgattagaag
    27301 cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg
    27361 taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag
    27421 aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca
    27481 taacccaccc ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca
    27541 gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc
    27601 tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat
    27661 ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg
    27721 ccagttacgg tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc
    27781 agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct
    27841 aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg
    27901 ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt
    27961 tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa
    28021 tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt
    28081 ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc
    28141 tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca
    28201 atagtcatat tgctcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca
    28261 ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg
    28321 atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg
    28381 ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag
    28441 acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg
    28501 ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg
    28561 ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca
    28621 ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa
    28681 catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt
    28741 ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct
    28801 gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg
    28861 ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg
    28921 atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc
    28981 gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac
    29041 attaattaaa tttacatttc tgcatggtta tgcataacca tgcagaattt ctcgctactt
    29101 ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc
    29161 tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg aatctttatt
    29221 agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga
    29281 acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata
    29341 tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg
    29401 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    29461 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    29521 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    29581 taccatggca gaacgtgcag tgcagctggg cggtgtagct ctggggacca ctcaagttat
    29641 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    29701 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    29761 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    29821 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    29881 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    29941 cttatttagt gcatccatct gctatctcca gctgattaag taaatttttt gtatccacat
    30001 catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    30061 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    30121 tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca aggagttgca
    30181 aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga
    30241 aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggctt
    30301 ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg
    30361 ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg
    30421 ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca
    30481 ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg
    30541 agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg
    30601 tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg
    30661 ttaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc
    30721 gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg
    30781 ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca
    30841 aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg
    30901 acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca
    30961 acgaaatcta caaaaaatgg ttcggtactg aaccgaaata ataacgctac acctgtaaag
    31021 cgcactggca gttccctctc ccctctgggg agaggattag ggtgaggggc gcaaacccgc
    31081 tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata
    31141 tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa
    31201 tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag
    31261 gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgagg
    31321 tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ttatttcgcc ctgccgatgg
    31381 cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact
    31441 ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctattcac aaaggttttc
    31501 gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac
    31561 cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag
    31621 acacctcgct gtttattgtg atcggcgtgg cggaactgac ccgtcagggg caagaaatta
    31681 ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta
    31741 ttaccctggt gctaagcttt attttgcgtc gtctggaaag aaggatgaaa atcctgtgat
    31801 tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgcttcaca atatcgattt
    31861 gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac
    31921 cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg
    31981 cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt
    32041 gttccagcag ttttacctct tcccgcatct gacggcgctg gaaaacgtca tgtttggtcc
    32101 gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc
    32161 gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca
    32221 gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga
    32281 accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tgcaggatct
    32341 ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt
    32401 agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggcg atccgcaggt
    32461 attgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaact
    32521 tctctgcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    32581 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    32641 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    32701 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    32761 gaacaaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    32821 aaagaattga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    32881 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    32941 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    33001 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    33061 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgctg
    33121 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    33181 cttccggcga tgattatcgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    33241 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    33301 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    33361 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    33421 tactggagtc gtcgcctgag ctggttaagc agtttgatag gttatggcct gattgtggcc
    33481 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    33541 atgctgtgca tgaccgtctg ggcgttgtac ctgatctttc gtaataaaaa agagattacc
    33601 cagcatttgc tcaactttgc ggagcattcg ctggcctttt tcagcctgtt tatccgcgcc
    33661 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcgtgctgtt tttcttttcg
    33721 ttgttcgatc cgggcaacag cctgaaattt atgatgggtg caacggtgcg cagcctggcg
    33781 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc aaaaaccatc
    33841 actctctcgc cacatactca gcgtaactat ccagagttgc aaaaacggct gaatggctgg
    33901 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcggt aatgttgctg
    33961 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    34021 accgtagata tcctgatccg tatcgcactt attctttttt tctcggcggt tggctggacg
    34081 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    34141 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    34201 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    34261 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt gaaagatatc
    34321 attaccgggg tgtttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    34381 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    34441 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    34501 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    34561 aatcaggcac tgaaagatgc ggtagcggaa ttaatggaaa acgaagaaat tcgcgggctg
    34621 attattggtg aaccgaattt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    34681 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    34741 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    34801 ctgcctactc cgggcgcgac cccggctgaa ccgttaccgc cgggggagcc aacgctttaa
    34861 cgctggcgat tgacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    34921 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    34981 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    35041 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa ggcgacttca
    35101 ccaccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    35161 agccccaggt tacggcgttt acgctcacta cctgcccgcg ctgcagcggc ggaatcgtgg
    35221 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tatggatgat gccgttaaaa
    35281 attgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    35341 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    35401 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    35461 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    35521 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    35581 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    35641 ttggcaaagt ctacgctttg ctccccggcg ggtgtaagcg tgaggaattg cctgagttca
    35701 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    35761 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    35821 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    35881 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattaatt tgtcttattt
    35941 tgtaaaaata tatcgttcaa aaaataagca catctctgtc aattttttgc gtcatttgca
    36001 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    36061 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    36121 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    36181 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    36241 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttcggtac
    36301 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    36361 gcctgttt
//
LOCUS       DAJTVE010000029        36294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000029 DAJTVE010000000
VERSION     DAJTVE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OO439_001369"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OO439_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6315774.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OO439_001370"
     CDS             1867..1992
                     /locus_tag="OO439_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315775.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OO439_001371"
     CDS             complement(1989..2060)
                     /locus_tag="OO439_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315776.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OO439_001372"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OO439_001372"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6315777.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO439_001373"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO439_001373"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6315778.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OO439_001374"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OO439_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6315779.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO439_001375"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO439_001375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6315780.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPVAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO439_001376"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO439_001376"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6315781.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO439_001377"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO439_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6315782.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OO439_001378"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OO439_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6315783.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OO439_001379"
     CDS             complement(9465..9872)
                     /locus_tag="OO439_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6315784.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO439_001380"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO439_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6315785.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO439_001381"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO439_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6315786.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OO439_001382"
     CDS             complement(11538..12197)
                     /locus_tag="OO439_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6315787.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OO439_001383"
     CDS             complement(12361..12672)
                     /locus_tag="OO439_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6315788.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OO439_001384"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OO439_001384"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6315789.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OO439_001385"
     CDS             complement(14207..14950)
                     /locus_tag="OO439_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6315790.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGGRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO439_001386"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO439_001386"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6315791.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO439_001387"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO439_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6315792.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO439_001388"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO439_001388"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6315793.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OO439_001389"
     CDS             20005..20088
                     /locus_tag="OO439_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315794.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO439_001390"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO439_001390"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6315795.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO439_001391"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO439_001391"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6315796.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO439_001392"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO439_001392"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6315797.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO439_001393"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO439_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6315798.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OO439_001394"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OO439_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6315799.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OO439_001395"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO439_001395"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OO439_001396"
     CDS             25234..25836
                     /locus_tag="OO439_001396"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6315800.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OO439_001397"
     CDS             complement(25937..25996)
                     /locus_tag="OO439_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6315801.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO439_001398"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO439_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6315802.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO439_001399"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO439_001399"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6315803.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO439_001400"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO439_001400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6315804.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OO439_001401"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OO439_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6315805.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OO439_001402"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OO439_001402"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6315806.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OO439_001403"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OO439_001403"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6315807.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OO439_001404"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OO439_001404"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6315808.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OO439_001405"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OO439_001405"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6315809.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36294)
                     /locus_tag="OO439_001406"
     CDS             complement(35716..>36294)
                     /locus_tag="OO439_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6315810.1"
                     /translation="IFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQH
                     VSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMI
                     DANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRP
                     MYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8690 a         9737 c         9164 g         8703 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccacgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggcccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaat
//
LOCUS       DAJTVE010000030        35779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000030 DAJTVE010000000
VERSION     DAJTVE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO439_001407"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO439_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO439_001408"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO439_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6315811.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO439_001409"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO439_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6315812.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO439_001410"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO439_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6315813.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO439_001411"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO439_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6315814.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO439_001412"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO439_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6315815.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO439_001413"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO439_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6315816.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO439_001414"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO439_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6315817.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO439_001415"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO439_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6315818.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO439_001416"
     CDS             complement(10994..11137)
                     /locus_tag="OO439_001416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315819.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO439_001417"
     CDS             11138..11416
                     /locus_tag="OO439_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315820.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO439_001418"
     CDS             complement(11463..13559)
                     /locus_tag="OO439_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6315821.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO439_001419"
     CDS             complement(13559..14296)
                     /locus_tag="OO439_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6315822.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO439_001420"
     CDS             complement(14293..14931)
                     /locus_tag="OO439_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6315823.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO439_001421"
     CDS             complement(15045..15287)
                     /locus_tag="OO439_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315824.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO439_001422"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO439_001422"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6315825.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO439_001423"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO439_001423"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6315826.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO439_001424"
     CDS             19524..19796
                     /locus_tag="OO439_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6315827.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO439_001425"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO439_001425"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6315828.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO439_001426"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO439_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO439_001427"
     CDS             22056..22862
                     /locus_tag="OO439_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6315829.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO439_001428"
     CDS             22872..23279
                     /locus_tag="OO439_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6315830.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO439_001429"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO439_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO439_001430"
     CDS             23839..24636
                     /locus_tag="OO439_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6315831.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO439_001431"
     CDS             24647..25471
                     /locus_tag="OO439_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6315832.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO439_001432"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO439_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO439_001433"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO439_001433"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6315833.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO439_001434"
     CDS             complement(27914..29545)
                     /locus_tag="OO439_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6315834.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO439_001435"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO439_001435"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6315835.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO439_001436"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO439_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6315836.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35779
                     /gene="espL4"
                     /locus_tag="OO439_001437"
                     /pseudo
     CDS             34789..35779
                     /gene="espL4"
                     /locus_tag="OO439_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9231 c         9100 g         8850 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaaggatc
//
LOCUS       DAJTVE010000031        34971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000031 DAJTVE010000000
VERSION     DAJTVE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO439_001438"
     CDS             complement(<1..815)
                     /locus_tag="OO439_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6315837.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO439_001439"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO439_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OO439_001440"
     CDS             complement(1397..1873)
                     /locus_tag="OO439_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6315838.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO439_001441"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO439_001441"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6315839.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OO439_001442"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OO439_001442"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6315840.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OO439_001443"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OO439_001443"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6315841.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OO439_001444"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OO439_001444"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6315842.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OO439_001445"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OO439_001445"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6315843.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OO439_001446"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OO439_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6315844.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO439_001447"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO439_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6315845.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OO439_001448"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OO439_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6315846.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OO439_001449"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OO439_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6315847.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OO439_001450"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OO439_001450"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6315848.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OO439_001451"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OO439_001451"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6315849.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEAVRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OO439_001452"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OO439_001452"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6315850.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OO439_001453"
     CDS             13848..14552
                     /locus_tag="OO439_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6315851.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OO439_001454"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OO439_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OO439_001455"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OO439_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO439_001456"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO439_001456"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6315852.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OO439_001457"
     CDS             complement(17484..18245)
                     /locus_tag="OO439_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6315853.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OO439_001458"
     CDS             18378..18788
                     /locus_tag="OO439_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6315854.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OO439_001459"
     CDS             complement(18750..19856)
                     /locus_tag="OO439_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6315855.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OO439_001460"
     CDS             complement(19867..21000)
                     /locus_tag="OO439_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6315856.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OO439_001461"
     CDS             complement(20993..22729)
                     /locus_tag="OO439_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6315857.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO439_001462"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO439_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6315858.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO439_001463"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO439_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6315859.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OO439_001464"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OO439_001464"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6315860.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO439_001465"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO439_001465"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6315861.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO439_001466"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO439_001466"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6315862.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO439_001467"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO439_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6315863.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO439_001468"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO439_001468"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6315864.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO439_001469"
     CDS             complement(31413..31673)
                     /locus_tag="OO439_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6315865.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO439_001470"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO439_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6315866.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO439_001471"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO439_001471"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6315867.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34971)
                     /locus_tag="OO439_001472"
                     /pseudo
     CDS             complement(32997..34971)
                     /locus_tag="OO439_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8272 a         8969 c         9487 g         8243 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc ggtacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa c
//
LOCUS       DAJTVE010000032        33996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000032 DAJTVE010000000
VERSION     DAJTVE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2602)
                     /gene="acrF"
                     /locus_tag="OO439_001473"
                     /pseudo
     CDS             complement(1..2602)
                     /gene="acrF"
                     /locus_tag="OO439_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="OO439_001474"
     CDS             complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="OO439_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6315868.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4170..4832
                     /gene="acrS"
                     /locus_tag="OO439_001475"
     CDS             4170..4832
                     /gene="acrS"
                     /locus_tag="OO439_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6315869.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4835..5014)
                     /locus_tag="OO439_001476"
     CDS             complement(4835..5014)
                     /locus_tag="OO439_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6315870.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5098..5982)
                     /gene="yhdJ"
                     /locus_tag="OO439_001477"
     CDS             complement(5098..5982)
                     /gene="yhdJ"
                     /locus_tag="OO439_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6315871.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6068..6364)
                     /gene="fis"
                     /locus_tag="OO439_001478"
     CDS             complement(6068..6364)
                     /gene="fis"
                     /locus_tag="OO439_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6315872.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6390..7355)
                     /gene="dusB"
                     /locus_tag="OO439_001479"
     CDS             complement(6390..7355)
                     /gene="dusB"
                     /locus_tag="OO439_001479"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6315873.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7684..8565)
                     /gene="prmA"
                     /locus_tag="OO439_001480"
     CDS             complement(7684..8565)
                     /gene="prmA"
                     /locus_tag="OO439_001480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6315874.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8577..10028)
                     /gene="panF"
                     /locus_tag="OO439_001481"
     CDS             complement(8577..10028)
                     /gene="panF"
                     /locus_tag="OO439_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6315875.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10018..10260)
                     /locus_tag="OO439_001482"
     CDS             complement(10018..10260)
                     /locus_tag="OO439_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6315876.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10369..11718)
                     /gene="accC"
                     /locus_tag="OO439_001483"
     CDS             complement(10369..11718)
                     /gene="accC"
                     /locus_tag="OO439_001483"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6315877.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLLEK"
     gene            complement(11729..12199)
                     /gene="accB"
                     /locus_tag="OO439_001484"
                     /gene_synonym="fabE"
     CDS             complement(11729..12199)
                     /gene="accB"
                     /locus_tag="OO439_001484"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6315878.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13177..14151)
                     /locus_tag="OO439_001485"
     CDS             complement(13177..14151)
                     /locus_tag="OO439_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6315879.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14303..16243
                     /gene="csrD"
                     /locus_tag="OO439_001486"
     CDS             14303..16243
                     /gene="csrD"
                     /locus_tag="OO439_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6315880.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16548..17591
                     /gene="mreB"
                     /locus_tag="OO439_001487"
     CDS             16548..17591
                     /gene="mreB"
                     /locus_tag="OO439_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6315881.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17657..18760
                     /gene="mreC"
                     /locus_tag="OO439_001488"
     CDS             17657..18760
                     /gene="mreC"
                     /locus_tag="OO439_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6315882.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18760..19248
                     /gene="mreD"
                     /locus_tag="OO439_001489"
     CDS             18760..19248
                     /gene="mreD"
                     /locus_tag="OO439_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6315883.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19257..19850
                     /gene="yhdE"
                     /locus_tag="OO439_001490"
     CDS             19257..19850
                     /gene="yhdE"
                     /locus_tag="OO439_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6315884.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19840..21309
                     /gene="rng"
                     /locus_tag="OO439_001491"
     CDS             19840..21309
                     /gene="rng"
                     /locus_tag="OO439_001491"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6315885.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21377..25177
                     /gene="yhdP"
                     /locus_tag="OO439_001492"
     CDS             21377..25177
                     /gene="yhdP"
                     /locus_tag="OO439_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6315886.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25333..26778
                     /gene="tldD"
                     /locus_tag="OO439_001493"
     CDS             25333..26778
                     /gene="tldD"
                     /locus_tag="OO439_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6315887.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26906..27771)
                     /locus_tag="OO439_001494"
                     /pseudo
     CDS             complement(26906..27771)
                     /locus_tag="OO439_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27954..28157
                     /locus_tag="OO439_001495"
     CDS             27954..28157
                     /locus_tag="OO439_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6315888.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28165..29097
                     /gene="aaeA"
                     /locus_tag="OO439_001496"
     CDS             28165..29097
                     /gene="aaeA"
                     /locus_tag="OO439_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6315889.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29103..31070
                     /gene="aaeB"
                     /locus_tag="OO439_001497"
     CDS             29103..31070
                     /gene="aaeB"
                     /locus_tag="OO439_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6315890.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31162..31434
                     /locus_tag="OO439_001498"
     CDS             31162..31434
                     /locus_tag="OO439_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6315891.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31490..31753)
                     /gene="yhcN"
                     /locus_tag="OO439_001499"
     CDS             complement(31490..31753)
                     /gene="yhcN"
                     /locus_tag="OO439_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6315892.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32118..32588)
                     /gene="argR"
                     /locus_tag="OO439_001500"
     CDS             complement(32118..32588)
                     /gene="argR"
                     /locus_tag="OO439_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6315893.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33023..33961
                     /gene="mdh"
                     /locus_tag="OO439_001501"
     CDS             33023..33961
                     /gene="mdh"
                     /locus_tag="OO439_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6315894.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8255 a         8453 c         8900 g         8388 t
ORIGIN      
        1 ttcctgatac gacatacctg tccagtcata accaatgccc gcaggtaatt ttgacgcaag
       61 gttttccatc aatgccatgg catcgccgga actggttcct ggcgcggctt ccccctgaat
      121 ctccattgac ggcagaccgt tgtagcgttc cagtcgcgga gagccataca cccaatgtga
      181 agtggtaaag gcagagaatg gcaccatttc gccgttggcg ctgcggacat aaagtttatc
      241 gacatcttct ggcagcatac ggaatttggc atccgcctga acatacacct ttttcacgcg
      301 accacggtcg atgaagtcgt taacgtaagt cccacccagc gccgttgaaa tggtctgatt
      361 gatgtcagaa agtgaaacac ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa
      421 ctgcgcggtg tcttccagac cattaggacg cacgctgact aagctggcag gatgttgcgc
      481 cgccatgcca agcaactggt tacgggcctg ggtcagggca tcgtgaccca gcccagcctg
      541 atcaattaac tcaaagtcga aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt
      601 gaatggaatg acaaaaccgt cgcggatctt gcccaattcc attttggcac gatggattac
      661 cgcttccgca ctgttttcgt caccactacg ctcttcccac ggtttcagac tgacgaaagc
      721 cataccggcg ttttgtgcct ggccgctgaa gctaaagccg ttaaccgtaa agacactttc
      781 tacgttcgct ttctcgttat tcagatagta atccgtcact tgatccaaca ctttttgcgt
      841 ccgctcttgc gtcgcgccag cgggtaactg aatcatggtc agaaagacac cctgatcctc
      901 ttcaggtaag aaggaagaag gaagacggaa gacgtaaaaa caacaccacc attcctgcaa
      961 caatcagcgc atagatcagt aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt
     1021 tggtgtagtg gttaacgcta tgatcgaagg tggtattaaa ccagccgaag aaaccgccct
     1081 tattttcgtg atgctcagca gagacgggtt taagcagcgt tgcacataac gcaggggtaa
     1141 gaatcaatgc caccagaaca gaaagcgcca ttgccgaaac gatggtgata gagaactggc
     1201 gataaattgc cccagtagaa ccgccgaaga atgccatcgg aataaatacc gctgacagca
     1261 ccatcgcgat acccaccagt gcgccctgaa tttgcgacat cgatttttcc gtcgcttctt
     1321 ttggcgggag cttatcctcc atcatcacgc gctcgacgtt ctccaccacc actatcgcat
     1381 catcgacgag cagcccgatg gcaagcacca tcccgaacat cgttagtgtg ttgatggagt
     1441 aaccaaaagc ggcgaggatg gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg
     1501 ggatcagcgt tgctcgcata ttctgcaaga acagatacat caccaggaac accagcataa
     1561 tggcttcgaa cagcgttttt accacttcgt gaatagaaag ctggacgaat ggcgtggtgt
     1621 cataagggta gagaaccttc attccctgcg ggaagaatgg ctgtaattcc gccagttttg
     1681 ccttaatggc tttcgcggta ttgagagcgt tcgcgccggt tgctagctta atccccaggc
     1741 ccgccgccgg ttttccgttg atacgagcga taacgttata gttttcaccg ccaagttcaa
     1801 ctcgtgcgac atctttcagg cgtaccaccg agccgtcact gtttacgcgc agggttactt
     1861 tgccgaattc ttccggattt tttaaccgcg tctgagcaat aatcgaggcg ttcaactgtt
     1921 gccctggtaa cgctggcgtt ccgcccaact gcccggcagc gatctgatcg ttctgtacct
     1981 tcaactggtt aatcacatca accggtgtca gtttatattt gtttagcaga tcggcgtcca
     2041 gccagatacg catcgcatac tgtgcgccga aaagctgtac gtcaccgacg ccattcagac
     2101 ggctaagcgt atctttaacg ttagaggcca catagtccga gatatcgtcc tgtgtggtgt
     2161 ctgggttatc agagacaaag cccgccacca tcaaatagct gctactggac ttttcaacac
     2221 tgatcccctg ctgctgaacc tcctgcggca gcaacggcgt ggcgagctgg agtttgttct
     2281 gcacctgcac ttgcgcgata tcaggatcgg tcccggactg gaaggtaagg gtaattgtca
     2341 cgctaccggc ggaatcgctg gtggaggaca tatacatcag gttatcgata ccattcatat
     2401 tctgttcgat aacctgcgtc accgtatcct gcacggtctg cgcatcagca cctggatagt
     2461 ttgcctaaac agaaaccgca ggcggtgcaa tggttggata ctgagcgacg ggcaattgta
     2521 ggatcgccag tgcgcccgcc atcatcagaa taatggccag cacccatgca aatatcggtc
     2581 gtcgaataaa aaagtttgcc atgtcagatt accttacttc gatgcagtat ctgcgggggt
     2641 atcggtagtg gctttaacct gctctcccgg acgcgctttt tgcaggccgc tgacaatgac
     2701 ttgatcgcca gatttcagac cttcgctaat caaccattta tcgccaatcg cctgactggc
     2761 aacgacaggg cgcgcttcca cctgactttt atcgttaaca atcagcacgg ttgcatcacc
     2821 acgcggtgtg cggctaacgc cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc
     2881 atcaatccgt gcacgcacaa acatacccgg caaaagcgta tgttgcgggt tagggaagac
     2941 agcacgtagg gttatggagc cggtgcattc atcaacggtc acatcagaga attgcagcgt
     3001 acctttcagg ggataggttt gaccgttttc catgaccaac tctacgttgc tggtggcgtt
     3061 ttccttatgc aaatttcctt gctctacgga ttgcttcagc ctcataaaat cgttgctgga
     3121 ttgggtcaca tcaacgtaga taggatcgag ctgctggaca gtcgccagtt cagtcgtttg
     3181 cccattagtg acaagagcgc cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg
     3241 cgcagtcact ttggtataag cgagattgat gcgagcgctt tcgactgtgg cttttgcggc
     3301 aatcacggcg gcatcggcct ggcgagcatc agcaatggcc tggtcgtact cctgctggct
     3361 gatgtatttc gtacccacga gcggaacgta acgttttacc gtcaaatgcg cgatggcggc
     3421 ggcggcttca cttttcgcca gttcgccttt cgcgctgtca taatttgcct gataggtcgc
     3481 gggatcgatc tggtacaggg actggcctgc ttgtacatcg ctgccttcag tgaaattacg
     3541 attcagtacg atcccgctaa cctgtgggcg aacttcggct atacggtaag cattggtgcg
     3601 gcctggtaat tcagtcttaa cttctaacgg ggccgttttc acaatatgaa cggtaacctg
     3661 cggttcaccg acgtgagctt tctcttctcc cttatcgtta caaccgacga ttaaagccgc
     3721 ggagatcaga ataaaggagg gcaggaggaa aaacctggca tgtttcgtca ttactattcc
     3781 tcaaaaaaac aaaagcgcgt tatttaccca gaaaggcaac gcgttaactc gcagaaagaa
     3841 aaatacagtt tgctatccta caaattatca ttcgtcgatg taaggaatag ttatgaatac
     3901 tggcgtctca aggcacataa acacaaaaaa agattaatat tctactgttt tattttgacg
     3961 cgggttgaaa gaggcagaat taaaacctcg taaattgaaa tatatattca tgtggcgaat
     4021 gtattttagg taaataatat atattattta tttgacgatt tatagcaact caatgttacc
     4081 ctctcgtata aatacacatt agatgataga ttaaccttcg ctattttctc accctgtgtc
     4141 gaatatattt atttcctgaa taattaatca tggcaaaaag aaccaaagcc gaagctctga
     4201 agacccggca agaactgatt gaaactgcca tcgcccagtt tgcgcaacat ggcgtaagca
     4261 agacgacgtt caacgacatt gccgatgccg ctaacgttac gcgtggcgct atctactggc
     4321 acttcgagaa caagacccaa ctgtttaatg agatgtggtt gcaacagcct tcattgcggg
     4381 agttaatcca ggaacacttg acggctggat tagagcatga cccgtttcaa caattgcgtg
     4441 aaaaattgat tgtcggcttg caatatattg ccaaaattcc ccgccagcag gcgttgctga
     4501 aaatcttata tcacaaatgt gaatttaatg atgggatgct ggccgaggga gtgatacgcg
     4561 aaaagatggg ctttaatccg cagactctcc gcgaagtatt gcaggcgtgt cagcaacaag
     4621 gttgtgtagc aaataacctc gatttagatg ttgtgatgat tattattgat ggtgccttca
     4681 gcggaattgt tcaaaactgg ttaatgaata tggcgggtta tgatctttat aaacaagcac
     4741 ccgcgctggt cgataacgta ttaagaatgt tcatgccaga tgaaaacata acgaaattaa
     4801 ttcatcaaac aaatgaatta agtgtcatgt aaattcacat tctgtcctga aaattcagtt
     4861 cgtcacagtt aaccagtttt agtggataaa ctgagttcat attacggcat ttgtctgttt
     4921 ctgttgtcag cagttcaatc cactgcatca gtaaagtatc aaacagaaaa acgaagacag
     4981 cgaaaacgac gagccaccaa tacttgcgaa tcattacctg agcctgtgca tgtttaacca
     5041 agtctggcag gaaatataca cgaaatgaat aatctgaaaa ccaggcttac acttccttta
     5101 ctttgcaatg agatcggggt caacttcgct taaccggctt cgttttgaca ggttgccggt
     5161 ctttcttttt ttcactttcg ccagttcttc cgcggagtaa tgcgacgcga catccagccg
     5221 tcgaagcccc attttgatgt actcgctgtt gatctcaata ccaatgaatt ttcgtccgct
     5281 ggcgatggct acggcaccgg tagtaaagct accggcaaac gggtcgagaa cgatatcgcc
     5341 tgggttggaa gaggcgagaa taatgcgttt cagtaaggct tccggttttt gcgtcgggtg
     5401 gttctcatat tcatccatca aataacgcac gcgcggaaaa tcccagacgt tacccggtac
     5461 tttttgatga ttgtatggct gtggaggatt tttgcgataa tcgatcaacg cgcgctgcga
     5521 tccggttttg gcttctacca gaatagcatc accgttgaat gtgtagttct ttgcgtcttt
     5581 caccatcatc aggatgggtt cgtacatgga gccgtagtgt tttttcgcct gtactccaga
     5641 actgtcatat gaccagacga tgcgactttt gatggtaaaa agcttgcggc actggagatc
     5701 gataaagggc atgttttccg tactgttcat aatgtacatg ctgccctgct ttttcagaac
     5761 gcggtggcac tctacaatca cttcaaacag ccagtcgata aacagatctt ctttccaggc
     5821 ttcgatcaga ccatcaaaat ttttaccgat gttatacggt gggtcggcaa agatcagatc
     5881 gacgctttca gtaggtagct ttttaagttc ggcaagggca tcaccgtgaa taatggtctt
     5941 agcttcatta ccaaaccggg tcggttcaca tcctgttctc atggtcactc cctttgtgac
     6001 acctataaaa aaggcgcttc cccatgccga gtagcgcctt tttaatcaag catttagcta
     6061 acctgaatta gttcatgccg tattttttca attttttacg cagcgtacca cggttgatgc
     6121 ccatcatcag cgcagcacgg gtctggttac cacgggtgta ttgcatcacc atgtccaaca
     6181 ggggctgttc tacttcagcc agtaccagct catagaggtc attcacatcc tgaccattca
     6241 gttgagcaaa atagttcttc agtgcctgtt taaccgagtc acgcaggggt ttttgggtta
     6301 cctgatcctg agagttaacg gtagaaacgg tcagtacgtc agaatttacg cgttgttcga
     6361 acatagttct gtcagctctt tatttctgtt tacgcaaaat tttcgaagta tgcctccaac
     6421 gcctccagct gttcgctggc atcctcaatg gcgttgaatg tgcgccgaaa ctggtcattt
     6481 ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg caattcggta cccttttgcc
     6541 ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa gcaagcgctt aacctctgcc
     6601 aaaggcagcg ggggcagcaa ctccccagtg tccagataat gctggatttc ccgaaagatc
     6661 cagggtcttc cctgagctgc gcggcctatc atcagggcat ccgcccctgt atagtcgagc
     6721 acagctctgg ctttaagcgg gtcagtaatg tcgccattcg cgataaccgg aatggaaact
     6781 ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc cattgaacaa acaggcgcgt
     6841 gtacggccat gaatggtcag agcctgaatg ccacagtctt cagccagttg ggcaatctct
     6901 tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct tcagggtaac aggaacgtcc
     6961 actgcattga cgacctcggt aaggatcgat ttaacgacat ccgggtactg caagagggct
     7021 gaacctgcga gcttgcgatt cactttttta gccgggcaac ccatattgat atcaataatc
     7081 tgggcaccgc tttccacgtt aatacgtgct gcatctgcca tttctttcgg atcgctacca
     7141 gcaatttgca cggtgcgaat accgggttca tcaatgtgca ccatccgtaa acgagatttg
     7201 tcgctttccc aaacctgtgg gttagaagac atcatctcgg atactgtcaa tccggctccc
     7261 atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc cagccatggg cgctgcgatc
     7321 aggcgatttc tgagctgata ttgtccgatg cgcatgagtt aagaaatgac catactgtga
     7381 ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg aaaggccaaa ctttgaccaa
     7441 tcctctgaga tggatcaaag aattgcattt aaaatgagcg tggtgagata attactcata
     7501 aaaatcatca tattagaaaa tagtgactaa aatttacact caaagaaatt tgagtaagtt
     7561 ctcaattttt ctttatgaat gaaaattttg gcacgcaaat ttgcgtaaat aaccggcaaa
     7621 tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc gtcaccctac ggcgatgcga
     7681 aggttaattc ttacgaccgg taatgcggca ccactcttct ttttccacga ccgggtccag
     7741 tgcgaagctg tcgacataag cttcacaaac gctctctgcc tggcttgcca gaataccgga
     7801 aaggcccagc aaaccgcctg aaaccggcag gacgctgatt aacggtgcca gttcacgtaa
     7861 tgggcctgca aggatgttag cgaccaccac gtcggctttc atttcttctg gctgatcttt
     7921 cggtaagtag agttccagac ggtcagaaac gccattacgt tcggcgttgt cgcggctagc
     7981 ctgaatcgcc tgcggatcga tatcaatacc aatggctttt gctgcaccca gtttcagcgc
     8041 cgcgatagcc agaatgccgg aaccacagcc aaagtcgatg actattttac cggttaaatc
     8101 gaggctgtcg agccactgca ggcacagaga ggtagttgga tgggtacccg taccaaacgc
     8161 cagccctgga tctaacatca cgttgacggc gttttcgtcc ggcacatcac gccagctagg
     8221 gcagatccac agtcgttcac caaagcgcat cgggtggaaa ttatccatcc attcgcgctc
     8281 ccagtcttta tcttccagtt gttcgatttt atgcgcgaag cctgcgccga gcagcggatg
     8341 gttttccaga atcgccacca cgtcgttcat atcggtttca gcgtcgaaca gaccaatcac
     8401 atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc tcaaatactg gcgtatcgtg
     8461 ggtatcctga aaagtgatag aaacggcacc cgcttccatc agcgcatcgc taagatcttc
     8521 agcgttcgcg ccggtggtgt tcagtttcag ttggatccaa ggcatggcaa aactctttat
     8581 ttatcagtag tcaaaacggt agcttgcggg acggatgtac cgaaacggtt tccgaccagg
     8641 aaagccagca aacttagtag taacgagggc acgatagggt ggaagcccag gtactgaata
     8701 ttcagcgtcg cgagtacggc atacagcacg ccgccaacga tcatcgcact tagcgcgcct
     8761 ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg gccacaggaa aacggcttcc
     8821 agcccaccga aggccagcaa attcagccag atgatcattt ctggcggctt ccaggcggca
     8881 agcagcagca acgcgccgag aactaacgta attaccgccg acatccgctt cagacgcgtc
     8941 tcgttttgca tttgatccgg acggatattc agatagagat ctttaatgat cgtagcggaa
     9001 ctttgtagca attgggcgtt aattgtcgac atgatcgcag ccataggtgc agccagaaag
     9061 atcccggcag caaacggtgg cagcactttt accattaacg tcgggatcac cagatccggt
     9121 acggtgagat cggggatcac tgcacgacct aacgctccgg ctaggtgcat accgaacatc
     9181 aggattgcga ctacaatcgt accgatgatg atcccccggt gtaccgcttt gctgtcttta
     9241 taagagatac agcgcaccgc agtatgcggc aggccaatca cgccaaagca caccagtacc
     9301 cagaacgacg tcataaaggc aggcgacaga atatcgtcag cgccttgtgg cgtaaccagt
     9361 tgcggatcga tggtttgcaa ggtctgtact gcgttgctta agccgccagc ggcatgtacc
     9421 acgccaataa gcagcacaac ggtgccaatc agcatcacaa gcccttgcat ggtgtcgttc
     9481 agcacgctgg cgcgaaagcc gccaaaggcg gtatacaacg caatgctgat accgaaaatc
     9541 agcagcccgg tttcataagg aatacccgcc gcggtttcca gcaggcgcgc accgccgata
     9601 aactgcacgg tcattgcccc aacgaacgca accagcaaac tcaaactcgc cagccacacc
     9661 agaagacgac tctggtagcg ggcaaacagc atgtcgttca gcgtcactgc attgtagcgg
     9721 cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg aaagccagac tgcaggaagc
     9781 tgaatcatcg ccagcaatac ccagcccagc ccgtatttat aagcagctcc tggcccgccg
     9841 ataaacgaac tggcactgat ataggtcgcg gtgagcgtca tcgccagcac aataccgccc
     9901 atagagcggc tgccgaggaa atactcatta aggaaggtgc cggtgctccg tttacgcatc
     9961 gcataaaccg agataccgaa caccaccacc agataggcga ccagcggtag aattacttca
    10021 agctgcatcg tcatcctcca gtgggatatc gcgatagata aatttcacca tcgcccagca
    10081 cagtccaata aacagtagcg gcgtcaggat gcaggccatc tcaaaccagc gcggaaagcc
    10141 ggtaaaaccg ggggcaacgc caggtaagta agcggctact aaccaaactg ccagatacaa
    10201 aagggtcagc cccagcgccc agcgcgcctc tttatgggcc tgaacaaaac gagtgtccat
    10261 tttttgtccc tgatgggtga agaaagcgag gattgtacct tatgggggtt gtcgatcccc
    10321 agtaataaaa aaggccggaa aatccggcct tttggcgctt ttgcagtctt atttttccag
    10381 aagaccgagt tttttctcca gatagtggat gttagtgcca ccatgctgga agttctcgtc
    10441 attcatgatg cggatctgca gatcaacgtt ggttttgata ccgtcgatga tcagctcctg
    10501 cagcgcattc ttcatgcggg caatcgccac gtcacggttt tcaccgtagc aaatcagctt
    10561 accgatcatt gagtcatagt acggcggtac ggtgtagccc gcgtagatat gagactccca
    10621 acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt ttgcctgggc ttggcaggaa
    10681 ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca tggccgcgaa cgtgaacttc
    10741 ttcttgcttg atcgacagcg gttgaccggc agcgatacgg agctgttctt tgatcaggtc
    10801 aacgccagtg atcatttcgg taaccgggtg ttctacctga atacgggtgt tcatttcgat
    10861 gaaatagaac tcgccgtttt cgaacagaaa ctcgaaagta cctgcaccgc gatagccgat
    10921 atcaacacac gctttagcgc aacgttcgcc gatgtagcga cgcagttccg gggtaatgcc
    10981 cggtgctggc gcttcttcga ccactttctg gtggcggcgc tgcatggagc agtcacgttc
    11041 cgccagatag atagcgttgc cccgaccgtc agccagtacc tgaatctcga cgtggcgagg
    11101 attttccagg tatttctcca tgtaaaccat atcgttgctg aaagcagctt tcgcttccgc
    11161 acgggtcatg gagatggatt gtgccagttc agcgtcgccg cgcactacgc gcataccgcg
    11221 accaccgccg ccgccggagg ctttgataat caccggataa ccaatgcgtt tagcaatggc
    11281 acggttttta tccatatcgt cgcccaacgg gccgtcagaa cccggtacgc aagggacgcc
    11341 cgcttttttc atcgccgcga ttgcggatac tttgtcgccc atcaggcgaa tggtttctgc
    11401 tttcgggccg atgaagataa agccggagcg ttcaacctgc tcggcaaagt tggcgttctc
    11461 ggagaggaag ccgtaacccg gatggattgc tactgcgccg gtgatttcag cggcgctgat
    11521 gattgccggg atgttcagat aactttttac tgacggagca gggccaatac agaccgtttc
    11581 atctgccagt aatacgtgtt ttagatcgcg atccgcgctg gagtgcacag cgacagtctt
    11641 gatgcccagt tctttacagg cacgaagaat acgcaatgca atctcgccgc ggttggcaat
    11701 aacaatttta tccagcatgt tcgcctcgtt actcgatgac gaccagcggc tcgtcaaatt
    11761 ctaccggttg tccactttcg accagaattg ctttcacggt accggatttg tccgcttcga
    11821 tctggttcat cattttcatg gcttcaacga tgcacagggt atcgcccacg ttgactttct
    11881 gacccacttc gatgaacgct tttgcgtccg ggcttggggt gcggtagaaa gtaccaacca
    11941 tcggggaacg tacgatgtga ccactgattt ccgctgctgc tggcgcttcc atggaaggaa
    12001 cggtcgccgg agcggctgcg ttagattgag ctggctgctg catcattggt gcagcgtaag
    12061 cttgttgcat cacagggaaa cttgcggcag gagctgcacg gctaatgcgt actgactctt
    12121 cgccttcaga aatttccagt tcggagatgc ctgattcttc aaccagctcg atcagttttt
    12181 taatcttacg aatatccatg agtgggttcc gtactctttg tttagtgtga ttgtgacagg
    12241 cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg tatgcatagc gataaattcc
    12301 agcaggccgg gacgccggtc tattttgccc cagagtatca atgttagact tgacggacat
    12361 tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa tataccttcg ttgcgccaat
    12421 gtcacctttt ccgcagcgca aaaactgcgt cagggaggac gaggtcgcac attataacga
    12481 tttcgtagca attggcagct aaatactggt ctcatcaggg aagataatca acagctaaca
    12541 tgtaaataac cttcaacacc gtgtaatttg caacaagccg cacaattcac gaaattagcg
    12601 ccatcatcga cggaacttct tataacgtaa ggtgaaaagc tgtaaagcca gccctgcgaa
    12661 gatgaggggc tgcaatgaga taatcttcac agaccacaaa taatgtaggg atgccaggat
    12721 cgcgacaaga tagacgacat tacgcagcgg ttgctgggga ttacccaatt ttcactgcat
    12781 cgtctggata gatgtgaatg ctaaagcacg caaaatcaac cgactgacaa ttctcagcat
    12841 taaataggtt cgagtggtta atttgtagtc aaataaaatc agattattca ccccatggta
    12901 ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc aaagcaccat aaacctaaca
    12961 ggcgacggag gcgtatcaat aatggctgtt tagaggagta tcaatgcgca tcaatttgtg
    13021 gtcagtcgaa atcatgactt tccagtttgg atgatgttgt acaacttcct gaccaaggtg
    13081 ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac cgattgaata atgatgtcaa
    13141 aatgagttcc ctctcttatt attccttgct aaatggttag ttaaccttca ccagcgtgcg
    13201 accctggatc tggttattaa tgatggcctc ggcaaagttc ggtgcctctg acagagatat
    13261 ctcttttgcc gcctgggtat agaatgattc cggtaaatcg gcgaccagtc gctgccaggc
    13321 ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc ccttgcaaac ggacattacg
    13381 cagaataaat ggcatgaccg tggttggcag agtaaaacca cccgccagac cacaggccgc
    13441 cacgcagccg ccgtaattca tttgcgccag cacttttgcc agcactttgt cgccaacagt
    13501 gtcaattgcc ccagcccaga cttgtttttc cagaggacgg gattcggcaa actcatcacg
    13561 agggagaata cggctggcac ctaaactttt cagatattca tgggtacttt cgcgaccgga
    13621 aacggcaacg acctgataac ccaacttatg cagcagcgcc acggcggtac tgccgacgcc
    13681 accactggca cccgtcacga caatctcccc gtcctgcggg cgaacaccgg catcttccag
    13741 cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg ataatcattg ctttacgcgc
    13801 gtccagccct tgcggcatgg caaccagcca gtcacctttc actcgcgcct gctccgccag
    13861 cccaccccag tggttttcac caacgcccca gccagtgagt aacacctcct gaccggcatg
    13921 aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa tcgatcccag gaatcatcgg
    13981 aaaattacgg atgatttttc ccttaccggt aatcgccagc gcatctttat agttcaggct
    14041 cgaccagtga acatcgaccg tgacatcgcc ctccggcagg cgactttcgt ccagagtctg
    14101 tactgatgcg agagttttgc cgtcctgctg ttctaaaagt aacgcctgca taagtggtcc
    14161 tcatgtgcat gatggattgg aaaataattt ctgaagatta tactcgctaa tggaaaagca
    14221 aagccgatga agcgcaataa attgcgagat aaatctgatt tgctagtatg cccgcttcct
    14281 cactatcgga gttaacacaa ggatgagatt aacgacgaaa ttttcggcct ttgttacgct
    14341 gctcaccggg ttaacaattt ttgtgacttt gctgggctgt tcgctaagtt tctacaacgc
    14401 aattcagtat aagtttagtc atcgcgttca ggcggtggcg acggcgattg atacccacct
    14461 tgtgtcgaat gacttcagca cattaaggcc acaaattacc gaattaatga tgtcggcaga
    14521 tatcgttcgt gtagacctgc tccatggtga taaacaggtt tataccctgg ccagaaatgg
    14581 tagttatcgt ccggttggct ccagcgatct gtttcgcgaa ctgagtgttc cgttgataaa
    14641 gcatccgggg atgtcgctgc gtctggttta tcaggatccg atgggcaact atttccattc
    14701 tttaatgact accgcgccgc tcacgggggc gattggcttt atcattgtta tgctcttcct
    14761 ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa ttgctggaaa cccgggctac
    14821 tcgtatctta aacggtgagc gtggctctaa tgtgttggga accatctatg aatggccgcc
    14881 cagaaccagc agtgcgctag atacgctgct tcgtgaaatt cagaacgcac gcgaacaaca
    14941 cagccgtctt gatacgctga tccgctctta tgccgcccag gacgtgaaaa ccggcctcaa
    15001 taaccgactc tttttcgata atcagttagc aacgttactg gaagatcagg agaaagtagg
    15061 tacccacggg atcgtgatga tgattcgtct gccggatttc aatatgttga gcgatacttg
    15121 ggggcacagc caggttgaag aacagttctt cactctgact aatctgctgt cgacatttat
    15181 gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc agtgattttg ctgcgctgtt
    15241 accgcaccgg acgttaaaag aggcagagag catcgccggt cagttaatca aagccgtcga
    15301 taccttgccg aacaataaaa tgctcgatcg cgacgatatg atccacattg gtatctgcgc
    15361 ctggcgtagt ggtcaggata ccgagcaggt aatggaacat gcagagtctg ccacgcgtaa
    15421 tgcgggattg cagggcggca atagctgggc tatttacgat gactcgttgc ctgaaaaagg
    15481 acgcggtaat gttcgctggc gtacgcttat cgagcaaatg ctcagtcgcg gcggcccgcg
    15541 cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt catcatcgcg aactcatgtg
    15601 ccgcatcttc gatggtaatg aagaggttag ctcggcggag tatatgccga tggtcttgca
    15661 gtttggctta tcggaagagt atgaccgtct gcaaatcagc cgtcttattc cactattgcg
    15721 ttactggcca gaggaaaatc tggcgattca ggttaccgtt gagtcgctga ttcgcccgcg
    15781 ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa aaatcacaac gaaaacgcat
    15841 aattattgaa cttgcagagg ccgatgtagg tcaacatatc agtcgtttac aacctgttat
    15901 tcgtttagtg aatgctttag gggtacgggt agccgtcaac caggctggtt tgacgctggt
    15961 aagcaccagt tggatcaaag aacttaatgt tgagttactc aagctccatc cggggctggt
    16021 cagaaacatt gagaagcgaa cggagaacca gctgctggtt caaagcctgg tggaagcctg
    16081 ctccgggacc agcacccacg tttacgccac cggcgtgcgt tcgcgaagcg agtggcagac
    16141 cctgattcag cgcggtgtta caggcgggca aggggatttt ttcgcgtcct cacagccact
    16201 tgatactaac gtgaaaaaat attcacaaag atactcggtt taacctgccg tttaatccgt
    16261 tttcacgtag aataacgcgc gctgcgtctc atgggagtgt gcttgtctgc tcgccagatt
    16321 gttgcagcac atatgcagat gaatgacctt acgcggttgc aaacaggcga ggaatgctgc
    16381 tgatgcatta agcctttctg gactcaggca gagatttgta acaaaggaaa cgaactgcac
    16441 taattttcac cgtagcagat gatttttgcg ccttgtcgct gctgcgtgtg gttggtaaag
    16501 taagcggatt ttcttttccg ccccagcttt caggattatc ccttagtatg ttgaaaaaat
    16561 ttcgtggcat gttttccaat gacttgtcca ttgacctggg tactgcgaat accctcattt
    16621 atgtaaaagg acaaggcatc gtattgaatg agccttccgt ggtggccatt cgtcaggatc
    16681 gtgccggttc accgaaaagc gtagctgcag taggtcatga cgcgaagcag atgctgggcc
    16741 gtacgccggg caatattgct gccattcgcc caatgaaaga cggcgttatc gccgacttct
    16801 tcgtgactga aaaaatgctc cagcacttca tcaaacaagt gcacagcaac agctttatgc
    16861 gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc gacccaggtt gaacgccgcg
    16921 caattcgtga atccgcgcag ggcgctggtg cccgtgaagt cttcctgatt gaagaaccga
    16981 tggctgccgc aattggtgct ggcctgccgg tttctgaagc gaccggttct atggtggttg
    17041 atatcggtgg tggtaccact gaagttgctg ttatctcctt gaacggcgtg gtttactcct
    17101 cttccgtgcg cattggtggt gatcgtttcg acgaagctat catcaactat gtgcgtcgta
    17161 attacggttc tctgatcggt gaagccaccg cagaacgtat caagcacgaa atcggttcgg
    17221 cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg ccgtaacctg gcagaaggtg
    17281 ttccacgcgg ttttaccctg aactccaacg aaatcctcga agcactgcag gaaccgctga
    17341 ccggtattgt gagcgcggta atggttgcac tggaacagtg cccgccggaa ctggcttccg
    17401 acatctccga gcgcggcatg gtgctcaccg gtggtggcgc actgctgcgt aaccttgacc
    17461 gtttgttaat ggaagaaacc ggcattccag tagttgttgc tgaagacccg ctgacctgtg
    17521 tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat gcacggcggc gacctgttca
    17581 gcgaagagta atcggatgca ggcaggggaa atgtctgttt accctgcctg gtctgatacg
    17641 agaatacgca taacttatga agccaatttt tagccgtggc ccgtcgctac agattcgcct
    17701 tattctggcg gtgctggtgg cgctcggcat tattattgcc gacagccgcc tggggacgtt
    17761 cagtcaaatc cgtacttata tggataccgc cgtcagtcct ttctactttg tttccaatgc
    17821 tcctcgtgaa ttgctggatg gcgtatcgca gacgctggcc tcgcgtgacc aactagaact
    17881 tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac agtgaactgc tgatgcttgg
    17941 acaatacaaa caggagaacg cgcgtctgcg cgagctgctg ggttccccgc tgcgtcagga
    18001 tgagcagaaa atggtgactc aggttatctc cacggttaac gatccttata gcgatcaagt
    18061 tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc cagccggtca tcagcgacaa
    18121 aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc agtcgcgtgc tgctgatttg
    18181 tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac gatatccgcg taattgcagc
    18241 cggtaacggt tgtacggatg atttgcagct tgagcatctg ccagcgaata cggatattcg
    18301 tgttggtgat gtgctggtga cctccggtct gggcggtcgt ttcccggaag gctatccggt
    18361 cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct tatactgtga ttcaggcgcg
    18421 tccgactgca ggactgcaac gtttgcgtta tctgctgctg ctgtggggcg cggatcgtaa
    18481 cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt gctaatgaac gtctgatgca
    18541 gatgatgccg caggtattgc cttcgccaga cgcgatgggg ccaaagttac ctgaaccggc
    18601 aacggggatc gctcagccga ctccgcagca accgacgaca ggaaatgcag ctactgcgcc
    18661 tgctgcgccc acacagcctg ctgctaatcg ctctccacaa agggctacgc cgccgcaaag
    18721 tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag tggcgagcta tcgtagccag
    18781 ggacgttggg taatctggct ctctttcctc attgcgctgt tgctgcaaat catgccctgg
    18841 ccggataacc tgattgtttt ccggccaaac tgggtgttac tcatcttgtt gtattggatc
    18901 ctggccttgc ctcatcgcgt aaatgtgggc acaggttttg tgatgggtgc catactggat
    18961 ctgatcagcg gctcgacgct tggcgtacgc gtattggcga tgagcatcat tgcttacctg
    19021 gtggcgctga aataccagct tttccgcaac ctcgcattat ggcagcaggc gctggtcgtc
    19081 atgttgcttt cgctggtggt ggatattatt gttttctggg cagagttttt agtgattaac
    19141 gtctctttca gaccggaagt gttctggagt agtgtagtca atggggtgct ctggccgtgg
    19201 attttcttgc tgatgcgtaa agtccgtcag cagtttgcag tgcaataaag gtttctatga
    19261 cttctctgta tttagcttcc ggttctccgc gtcgtcagga gttacttgcg caacttggtg
    19321 tgacctttga acgtattgtt acgggcattg aggagcagcg tcagccgcag gagagcgcgc
    19381 agcagtatgt tgtgcgtctg gcgcgcgaga aagcacaggc gggtgtcgcg caaacggcgc
    19441 aggatctccc ggtgctgggt gcggatacta tcgttatcct gaatggagaa gtgctggaga
    19501 aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa attatcgggt cagacccatc
    19561 aggtgatgac ggcagtggcg ttggccgaca gccagcacat tctcgattgc ctggtggtca
    19621 ccgatgtgac tttcagaacg ttaacagacg aagacatcgc gggctatgtc gccagcggtg
    19681 aaccgttaga taaagcaggt gcatacggta ttcaggggct gggtggctgt tttgtcagga
    19741 agataaatgg cagctatcac gccgtagtcg gcttacctct ggttgaaacg tatgaattat
    19801 taagtaattt taacgcactg cgtgagaaaa gggataaaca tgacggctga attgttagta
    19861 aacgtaacgc cttcggaaac gcgagtggcg tatattgatg gcggtattct gcaggaaatt
    19921 catattgaac gtgaggcgcg acgcggaata gtaggcaata tctacaaggg tcgtgtaagt
    19981 cgtgtacttc cgggtatgca ggcggctttt gtagatattg ggctggataa agccgcgttt
    20041 cttcatgcat ccgatatcat gccgcacacc gaatgtgtgg cgggtgaaga acaaaagcaa
    20101 ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg
    20161 gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca ccgatatcac gctgccttct
    20221 cgctatctgg tctttatgcc aggggcttct cacgttgggg tttcccaacg tattgaaagc
    20281 gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg
    20341 tttatcatcc gtaccgcagc ggaaggggtt ggcgaggctg aactggcctc cgatgccgct
    20401 tatctgaaac gcgtctggac caaagttatg gagcgtaaaa agcgcccgca gacccgttat
    20461 cagctgtacg gcgaactggc gctggctcag cgtgttctgc gtgatttcgc cgacgccgaa
    20521 ctggaccgca ttcgcgttga ctcacgcctg acttacgaag cattacttga gttcacctcg
    20581 gagtacattc ccgagatgac cagcaagctg gagcattaca ccggacgcca gccgattttc
    20641 gatctctttg atgtcgaaaa cgaaatccag cgagcgctgg aacgcaaagt agaactgaaa
    20701 tccggtggtt atctcattat cgaccagacc gaagcgatga ccaccgtgga catcaatacc
    20761 ggagcgtttg tcggtcatcg caatctggac gacaccattt tcaataccaa tattgaagcg
    20821 acgcaggcta tcgctcgcca gttacggttg cgtaatctgg gcgggattat cattattgat
    20881 ttcatcgata tgaataatga agatcaccgc cgccgcgtgc tgcactcgct ggagcaggcg
    20941 ttgagcaaag accgggtgaa aaccagcgtt aatggttttt cggcgctggg gctggtggag
    21001 atgacgcgta aacgcacccg cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc
    21061 tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag
    21121 attgttcgtg tccaccatgc ttacgactcc gaccgtttcc tggtctatgc ttctccggca
    21181 gtagccgaag ccttgaaagg cgaagagtca cactcgctgg cggaagtgga aattttcgtt
    21241 ggcaaacagg ttaaagtaca aattgaaccg ctctataacc aggagcagtt tgacgtcgta
    21301 atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt ttttgagtca catttttagc
    21361 agacaaggag tgacgggtga ggcgattgcc ggggatttta ctgcttactg gagccgcgct
    21421 cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt gctttaccgc atcttgacgc
    21481 ctggcgtccg gaaatcctca acaaaataga atccgcgact ggcatgccgg tagaagccag
    21541 tcagctctca gccagctggc agaattttgg cccgacgctt gaagcacacg acatccgtgc
    21601 agaactaaaa gatggcggcg aattttcggt taaacgcgtt actctggcgc tggatgtctg
    21661 gcagagcctg ttacatatgc gctggcagtt tcgcgacctc actttctggc agctgcgctt
    21721 tcgcaccaac actcctatca ccagcggtgg tagcgatgac agtctggaag ccagtcacat
    21781 cagcgatctg tttcttcgtc aatttgacca tttcgatctc cgcgacagtg aagtcagttt
    21841 cctgacgcca tccggtcagc gtgccgagct ggcgatccca caactcacct ggctgaacga
    21901 tccacgtcga caccgtgcgg aaggcctggt aagcctctcc agccttaccg gacagcacgg
    21961 cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg ttgttaagca atggtcgcgt
    22021 ctggctacag gcggatgaca tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa
    22081 tattgctctg gaaacggcac agttctccct tgaaggctgg atgacgatcg acaaaggcga
    22141 tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc agctggttgg gcgagaagca
    22201 aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt acgcgtgaaa atccgggctg
    22261 gcagttctct attcccgata cacggatcac gatggacggc aaaccctggc cgagcggagc
    22321 attgacgctg gcctggatac cggaacagga cgttggcggc aaagacaata aacgcagtga
    22381 cgaactccgg attcgcgcca gtaatctgga gctggcaggc ctggagggca tacgcccgct
    22441 ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc tccacacaac cgagcggcaa
    22501 gattaacact ctggcgctgg atatcccgct tcaggcggca gacaagaccc gttttcaggc
    22561 atcgtggagc gatctggcct ggaagcaatg gaaattatta ccgggtgcgg aacacttctc
    22621 cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa
    22681 gatgccttac gaaacggtat tccgtgcgcc actggaaatc gccgacggcc aggcaactat
    22741 aagctggctg aacaatgaca aaggtttcca gctggatggg cgtaatattg acgttaaagc
    22801 caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa cctgctaacg atgaaccctg
    22861 gctgggtatt ctggctggca tcagtaccga tgatggttca caagcctggc actatttccc
    22921 ggaaaacttg atgggtaaag acctggttga ttacttaagt ggcgcgattc agggcggtga
    22981 agcggataac gcgacgctgg tttatggtgg caatccgcaa ctcttcccct ataaacacaa
    23041 cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg aagtttgcct tccagccgga
    23101 ctggcctgca ttaactaacc ttgatattga actggacttt attaacgacg gtttatggat
    23161 gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc
    23221 tgactactcg aaagaaaaac tgctgattga cgctgacatt aaaggtccgg gtaaagccgt
    23281 tggcccttac tttgatgaga caccgctgaa agattctctg ggtgcgaccc tgcaagaact
    23341 ccagctcgac ggcgatgtga atgctcgctt acatcttgat atcccgctga acggcgagct
    23401 ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt ctgtttatca aaccactcga
    23461 cagcaccctg aaaaatttga gcggtaaatt cagctttatc aatggcgatc tgcaaagtga
    23521 accactgaca gcaagctggt ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg
    23581 ggcaaaagcc taccaggttg cggtgaatct caacggtaac tggcaaccgg cgaaaaccgg
    23641 cgttctgcct gaagcggtga acgaagcatt gagtggcagc gtggcgtggg atggtaaagt
    23701 gggcattgat ctgccttatc atgctggcgc gacctataac gtagagctga acggcgatct
    23761 gaataatgtg agtagtcact taccttcacc gttagccaaa cctgcgggtg aaccactagc
    23821 ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa ttaaccggac aggctggtgc
    23881 ggataaccat ttcaatagcc gctggttgct tggtcaaaag ctgacgctcg atcgtgctat
    23941 ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa caaagtggcg ttgaactcaa
    24001 tatgccgccg atgaatggtg ccgagtggct ggccctgttc cagaaaggcg ctgcggagag
    24061 tgtcggtggt gcagcgagtt tcccacaata cataacgtta cgtacgccta tgttgtcact
    24121 gggaaatcag caatggaata acctgagtat tgtttcgcaa ccgacggcaa atggcaccct
    24181 ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg atgcgtaata acgcgccgtg
    24241 gctggcgaat atcaaatatc tttattacaa cccgagcgtg gcgaaaactc gtggtgattc
    24301 aacgccgtca tcacctttcc cgacaacgga gcgcattaac ttccgtggct ggccggacgc
    24361 acaaatacga tgcacagagt gctggttctg ggggcaaaaa ttcggtcgca ttgacagtga
    24421 tatcaccatt tctggcgata cgttaacgct gaccaatgga ctgattgata ctggtttctc
    24481 gcggcttact gccgatggtg aatgggttaa taatccgggg aatgaacgta cctcgctgaa
    24541 aggaaaactg cgcgggcaga aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc
    24601 catacgccag tcgtcattta atgtggatta cgatttacac tggcgcaaag caccctggca
    24661 gccagatgaa gcgacgttga atggcatcat tcatactcaa ctgggtaaag gcgaaattac
    24721 cgaaatcaat accggacatg ccgggcaatt gctgcgctta ttgagcgtag atgccctgat
    24781 gcgtaagctg cgttttgatt tcagagacac ttttggcgaa gggttctatt ttgactccat
    24841 tcgcagcacc gcgtggatta aagacggggt tatgcacacc gacgacacgc tggtggatgg
    24901 cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg gtacgtcgcg acctgaatat
    24961 ggaagcggtt gtcgcaccag agatttctgc gacggtgggc gtggctgcgg cttttgcggt
    25021 taaccccatt gttggcgcgg cagtttttgc cgctagtaaa gtgctggggc cgctgtggag
    25081 caaagtctcc attttgcgct atcacatttc gggtccgctg gacgatccgc aaatcaacga
    25141 agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc
    25201 ccaatctcat atgataatcg ttgccaaagg ccaacgagcc agaacataac cgtaggccgg
    25261 atagggcgtt catgccgcat ccggcagccg taaaaaatcc tctactgcag taactaacga
    25321 gtagcaaaaa cgatgagtct gaacctggta agtgaacaat tgctagcggc gaacagcctg
    25381 aaacatcagg acttgttcgc gatcctcggt caactggccg aacgtcgcct tgattatggc
    25441 gatctctatt ttcagtcgag ctatcacgaa tcctgggttt tagaagaccg cattattaaa
    25501 gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc
    25561 ggatttgctt acgctgacca aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg
    25621 cgcaccatcg tccgtgatag tggtgatggc aaagtacaga cgctgggcgc ggtagagcat
    25681 agcccgttgt atacctcggt agatccgctg caaagcatga gccgtgaaga gaagctggat
    25741 atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact
    25801 gccagcctca gtggtgtcta tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg
    25861 gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa
    25921 cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt atgagttctt ccttgccgat
    25981 cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc
    26041 aatctttctg ccgttgctgc accagcgggc accatgccgg tagtacttgg cgcaggttgg
    26101 ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg aaggcgactt caaccgccgc
    26161 ggcacttcag tatttagtgg gcaggtcggg gagctggtgg cttcagaact gtgtaccgtg
    26221 gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg cgattgatga cgaaggtacg
    26281 ccaggccagt acaacgtgct gattgagaac ggcattctga aaggctacat gcaggataaa
    26341 ctcaacgcgc gtttgatggg gatgacgccg actggcaacg gtcgccgtga atcctacgcc
    26401 catctgccca tgccgcgtat gactaacacc tatatgctgc cgggtaaatc gaccccgcag
    26461 gaaattattg aatccgttga gtacggtatc tatgcaccga actttggtgg cggtcaggtg
    26521 gatatcacct ccggcaaatt cgttttctcc acttcagaag catatctgat tgaaaacggt
    26581 aaagtaacga agccggtgaa aggcgcaacg ttgattggtt ccggtatcga aaccatgcag
    26641 cagatttcga tggttggcaa cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa
    26701 gaagggcaaa gtttgccggt tggcgtgggc cagccaacgc tgaaagttga taacctgact
    26761 gttggcggta ctgcgtaata atttcattat tttcagaagg ataattaatc tttctacgtg
    26821 cacgaacggt cccctcgctc ctctggggtt agggtgaggg gaacccgttg gcacaggttt
    26881 gtagaacgta acagtacaat atgaattact tctctttccc gcgcccgtgc atctcctgaa
    26941 acaatttacc gacctcaaca aaataatccg tcagcgagtt gatcacgacc tgtaccttca
    27001 gcggcagctt atctttttcg gtatataacg cataaaccgg gcgtggatct gactgataac
    27061 gcggcagcag gatctccagc tcaccgcgat tgatctcgtt gatcacccac atcagcggca
    27121 cgtaggcgat cccggcaccc gccgtcagcc agcgcaccag cgtcatcgga tcattagtca
    27181 caaatcttcc ttgtgggatc aggcgagtcg agatcccttc cggtgcgatc agttcaaatt
    27241 cattgtcggg ccgcacgctg tattcaagcc atgaatgact actcaaatcg gcgggttttt
    27301 ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca caccaccatc ggcatcgcgc
    27361 ccagacggcg ggaaaacagg ctggaattcc ggtaaccaga ttgacgctca aacctgggta
    27421 ttctttcagc atttttgctg tcagcccggc gagaacattt tgtgccatag ttgaagaaca
    27481 gccaatgcgc agcgtcccga tgggggtgtt attgaaggca tacagttgct catgaacatc
    27541 ctgcacttca tgaagcatcc gacggcagcc ctggtagtaa attctgccgg cttcggtcag
    27601 gccaatgctg cgtgtgctac ggtttaacag ctttacctgc aactcatctt ccagttttga
    27661 caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga
    27721 gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca ttattcactc
    27781 tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt acgctaatat
    27841 attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg
    27901 cgacagatgc tgaaaataat aacgcctgct ctctctttac aaccaaggtc aacatgagtc
    27961 tgtttcccgt tatcgtggtg tttggactgt ccttcccacc gatatttttt gaattgcttt
    28021 tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact
    28081 ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat
    28141 cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc
    28201 atcacggtcg tattagtcat tctagccttc atcgcaattt ttaatgcctg ggtctattac
    28261 accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg
    28321 gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga
    28381 cagatactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc
    28441 gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac
    28501 cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa
    28561 acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt
    28621 gaacgcacgg tgatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc
    28681 ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa ctccttctat
    28741 gtactggcct atatggaaga aactaagctg gaaggggggc gtccggggta tcgtgcagag
    28801 atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgcaggg
    28861 gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgactataga ctctaatctt
    28921 gaatgggtgc gtcttgcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag
    28981 aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac
    29041 gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca
    29101 cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg
    29161 ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg
    29221 tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt
    29281 ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg
    29341 ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct
    29401 gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtacg
    29461 cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat
    29521 tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg
    29581 cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagta gatcgagagc
    29641 tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg
    29701 gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg caccacggca ctacaaggca
    29761 tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag
    29821 cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga
    29881 atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa
    29941 ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc tggaccgggg
    30001 aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc
    30061 tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag
    30121 gcgaaccgga agtaaaagta gagtcagccg aacgtcatca tgcaatggtt aacttctggc
    30181 gaaccacact ttcctgcatt ctgggcacgc ttttctggct gtggacgggc tggacttccg
    30241 gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc
    30301 cacgcatggt ggcgatcgac tttatctatg ggacgctggc cgcgctgccg ttagggctgc
    30361 tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc
    30421 tggcagtgct gggattcttc ctcggtatag aagtacagaa acggcgactg ggctcgatgg
    30481 gggcactggc cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca
    30541 gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta
    30601 ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg
    30661 tttttgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc
    30721 cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat
    30781 ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg
    30841 ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat
    30901 ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaactggaaa
    30961 tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc
    31021 ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg
    31081 acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc
    31141 gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt
    31201 caggaggatt tttatcgtga ctttagccaa acctttggtc tggctaaaga taaggtacgc
    31261 gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt
    31321 gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt
    31381 gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa
    31441 aaaaagcccc cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca
    31501 gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg
    31561 cgcctttctc ttccgctttt ttgttcagca tttcacgcat atccattggc gaagacgcca
    31621 caccacttac ggatacggtc ccgattgctt cacgattttg tgcttgtgca gtatcaatgg
    31681 agtcggcagc gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gctacagtgg
    31741 ttttgatttt catgattttt acctcgacat aatcttttag ctgggccttt gtttcgtgat
    31801 ccatatcaca aaatcaagca tacactaatt attgaactaa ttaataccac actaactgtt
    31861 tcagttgata ctaagtgtta aaaatgtgaa ttttaataac aaaaaagaat taaaaacacg
    31921 gttatttatt gttaattcta gtattttcaa ttagataatg aattttacat aatgtgcttt
    31981 ttttaccgta tgtattcatt gtgtgaatga tatgtcgcgg taaaacgcac tattcgttaa
    32041 attaatcgca ggggaaagca gggggttgag aggaataagc aacattttcc ccgccgccag
    32101 aagcgacggg gcagagatta aagctcctgg tcgaacagct ctaaaatcgc ttcgtacagg
    32161 tctttgacgg tgaaaccgtt agcaggggtg gtaaagatgg tgtcatcgcc agcgatggtg
    32221 cccagaatac cttctgcttt gcccagtgag tccagcaggc gagcaattaa ctgcgccgcg
    32281 ccagggctgg tatgaatcac gacaactgca tcgttgtagt cgatatccag caccagattc
    32341 ttcaatggac tggaggtggt tggtacaccc agttcagctg gcaggcagta aaccatttcc
    32401 attttggcat tgcgtgtacg tacagcacca aacttggtca acatccgcga gactttagac
    32461 tgattaatat tgtcaaagcc ttgctcctgc aacgcggcga cgatttcgcc ctgggagcta
    32521 aatttctctt ctttaagtaa tgctttaaat gctttaacta gttcttcttg cttagccgag
    32581 cttcgcataa gtcacccgat atggtggttg atacaacatt attgtgcata cagatgaatt
    32641 tttatgcaaa cagtcagccc tgaagaaggc tgaaataatg ttatgaaaga gcgggatttt
    32701 atcaaatttc gttattgaga aacatgcctg cgtcacggca tgcaaattct gtttaaaagt
    32761 aaattaattg ttatcaaatt gatgttgttt tggctgaacg gtagggtata ttgtcaccac
    32821 ctgttggaat gttgcgctaa tgcataagcg actgttaatt acgtaagtta ggttcctgat
    32881 tacggcaatt aaatgcataa acgctaaact tgcgtgacta cacattcttg agatgtggtc
    32941 attgtaaacg gcaattttgt ggattaaggt cgcggcagcg gagcaacata tcttagttta
    33001 tcaatataat aaggagttta ggatgaaagt cgcagtcctc ggcgctgctg gcggtattgg
    33061 ccaggcgctt gcactactgt taaaaaccca actgccttca ggttcagaac tctctctgta
    33121 tgatatcgct ccagtgactc ccggtgtggc tgtcgatctg agccatatcc ctactgctgt
    33181 gaaaatcaaa ggtttttctg gtgaagatgc gactccggcg ctggaaggcg cagatgtcgt
    33241 tcttatctct gcaggtgtag cgcgtaaacc gggtatggat cgttccgacc tgtttaacgt
    33301 taacgccggc atcgtgaaaa acctggtaca gcaagttgcg aaaacctgcc cgaaagcgtg
    33361 cattggtatt atcactaacc cggttaacac cacagttgcg attgctgctg aagtgctgaa
    33421 aaaagccggt gtttatgaca aaaacaaact gttcggcgtt accacgctgg acatcattcg
    33481 ttccaacact tttgttgcgg aactgaaagg caaacagcca ggcgaagttg aagtgccggt
    33541 tattggcggt cactctggtg ttaccattct gccgctgctg tcacaggttc ctggcgttag
    33601 ttttaccgag caggaagtgg ctgatctgac caaacgcatc cagaacgcgg gtactgaagt
    33661 ggttgaagcg aaagccggtg gcgggtctgc aaccctgtct atgggccagg cagctgcacg
    33721 ttttggtctg tctctggttc gtgcactgca gggcgaacaa ggcgttgtcg aatgtgccta
    33781 cgttgaaggc gatggtcagt acgcccgttt cttctctcaa ccgctgctgc tgggtaaaaa
    33841 cggcgtggaa gagcgtaaat ctatcggtac cctgagcgca tttgaacaga acgcgctgga
    33901 aggtatgctg gatacactca agaaagatat cgccttgggc gaagagttcg ttaataagta
    33961 attgattagc gaataataaa aaaccggagc acagac
//
LOCUS       DAJTVE010000033        33816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000033 DAJTVE010000000
VERSION     DAJTVE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..331
                     /locus_tag="OO439_001502"
                     /pseudo
     CDS             1..331
                     /locus_tag="OO439_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1877 family protein"
     gene            complement(398..1855)
                     /gene="nuoN"
                     /locus_tag="OO439_001503"
     CDS             complement(398..1855)
                     /gene="nuoN"
                     /locus_tag="OO439_001503"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6315895.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1862..3391)
                     /gene="nuoM"
                     /locus_tag="OO439_001504"
     CDS             complement(1862..3391)
                     /gene="nuoM"
                     /locus_tag="OO439_001504"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6315896.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3555..5396)
                     /gene="nuoL"
                     /locus_tag="OO439_001505"
     CDS             complement(3555..5396)
                     /gene="nuoL"
                     /locus_tag="OO439_001505"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6315897.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5393..5695)
                     /gene="nuoK"
                     /locus_tag="OO439_001506"
     CDS             complement(5393..5695)
                     /gene="nuoK"
                     /locus_tag="OO439_001506"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6315898.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5692..6246)
                     /gene="nuoJ"
                     /locus_tag="OO439_001507"
     CDS             complement(5692..6246)
                     /gene="nuoJ"
                     /locus_tag="OO439_001507"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6315899.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6258..6800)
                     /gene="nuoI"
                     /locus_tag="OO439_001508"
     CDS             complement(6258..6800)
                     /gene="nuoI"
                     /locus_tag="OO439_001508"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6315900.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6815..7792)
                     /gene="nuoH"
                     /locus_tag="OO439_001509"
     CDS             complement(6815..7792)
                     /gene="nuoH"
                     /locus_tag="OO439_001509"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6315901.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7789..10515)
                     /gene="nuoG"
                     /locus_tag="OO439_001510"
     CDS             complement(7789..10515)
                     /gene="nuoG"
                     /locus_tag="OO439_001510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6315902.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10568..11905)
                     /gene="nuoF"
                     /locus_tag="OO439_001511"
     CDS             complement(10568..11905)
                     /gene="nuoF"
                     /locus_tag="OO439_001511"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6315903.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(11902..12402)
                     /gene="nuoE"
                     /locus_tag="OO439_001512"
     CDS             complement(11902..12402)
                     /gene="nuoE"
                     /locus_tag="OO439_001512"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6315904.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12405..14207)
                     /gene="nuoC"
                     /locus_tag="OO439_001513"
     CDS             complement(12405..14207)
                     /gene="nuoC"
                     /locus_tag="OO439_001513"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6315905.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14301..14963)
                     /gene="nuoB"
                     /locus_tag="OO439_001514"
     CDS             complement(14301..14963)
                     /gene="nuoB"
                     /locus_tag="OO439_001514"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6315906.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(14979..15422)
                     /gene="nuoA"
                     /locus_tag="OO439_001515"
     CDS             complement(14979..15422)
                     /gene="nuoA"
                     /locus_tag="OO439_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6315907.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16051..16989)
                     /gene="lrhA"
                     /locus_tag="OO439_001516"
     CDS             complement(16051..16989)
                     /gene="lrhA"
                     /locus_tag="OO439_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6315908.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            17904..19121
                     /gene="alaA"
                     /locus_tag="OO439_001517"
     CDS             17904..19121
                     /gene="alaA"
                     /locus_tag="OO439_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6315909.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19205..19804
                     /gene="yfbR"
                     /locus_tag="OO439_001518"
     CDS             19205..19804
                     /gene="yfbR"
                     /locus_tag="OO439_001518"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6315910.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMLAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(19863..21695)
                     /locus_tag="OO439_001519"
     CDS             complement(19863..21695)
                     /locus_tag="OO439_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6315911.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21782..22430)
                     /gene="hxpA"
                     /locus_tag="OO439_001520"
                     /pseudo
     CDS             complement(21782..22430)
                     /gene="hxpA"
                     /locus_tag="OO439_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22441..22935)
                     /locus_tag="OO439_001521"
     CDS             complement(22441..22935)
                     /locus_tag="OO439_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6315912.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23018..23473)
                     /locus_tag="OO439_001522"
     CDS             complement(23018..23473)
                     /locus_tag="OO439_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6315913.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23811..25013
                     /gene="ackA"
                     /locus_tag="OO439_001523"
     CDS             23811..25013
                     /gene="ackA"
                     /locus_tag="OO439_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6315914.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25088..27232
                     /gene="pta"
                     /locus_tag="OO439_001524"
     CDS             25088..27232
                     /gene="pta"
                     /locus_tag="OO439_001524"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6315915.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27422..28942
                     /gene="yfcC"
                     /locus_tag="OO439_001525"
     CDS             27422..28942
                     /gene="yfcC"
                     /locus_tag="OO439_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6315916.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(28975..29517)
                     /gene="yfcD"
                     /locus_tag="OO439_001526"
     CDS             complement(28975..29517)
                     /gene="yfcD"
                     /locus_tag="OO439_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6315917.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29575..30126)
                     /gene="yfcE"
                     /locus_tag="OO439_001527"
     CDS             complement(29575..30126)
                     /gene="yfcE"
                     /locus_tag="OO439_001527"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6315918.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30182..30826)
                     /gene="yfcF"
                     /locus_tag="OO439_001528"
     CDS             complement(30182..30826)
                     /gene="yfcF"
                     /locus_tag="OO439_001528"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6315919.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            30962..31609
                     /gene="yfcG"
                     /locus_tag="OO439_001529"
     CDS             30962..31609
                     /gene="yfcG"
                     /locus_tag="OO439_001529"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6315920.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31666..32028
                     /gene="folX"
                     /locus_tag="OO439_001530"
     CDS             31666..32028
                     /gene="folX"
                     /locus_tag="OO439_001530"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6315921.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32049..32942
                     /locus_tag="OO439_001531"
     CDS             32049..32942
                     /locus_tag="OO439_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6315922.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(32990..>33816)
                     /gene="rpnB"
                     /locus_tag="OO439_001532"
     CDS             complement(32990..>33816)
                     /gene="rpnB"
                     /locus_tag="OO439_001532"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6315923.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8419 a         9248 c         8523 g         7626 t
ORIGIN      
        1 cttttgatcc cgcgaagaat gatccgctga gtcgtgccgt gcttggtgaa cacagcctcg
       61 aagatggcat agatggcttc ctgggattga cctggaatca ggagttagcc gcgacaatcg
      121 accgactgga atcactggat cgaagtgagc tgcgtaaaca gttttccatt aagcgactca
      181 atgaaatgga gatttatccg ggagtgacct tttcagaaga gttagagggg caactctttg
      241 cttcgatcat gctgatatgg aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg
      301 gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg
      361 aacatgtcgg cggctttctg acttacaaac tgatagctta catcagcggc attgctaacc
      421 gcacaatgct aatcagcggt tgtggccata cacccagcac cagtaccaac agtgcagaga
      481 tcagcaccac gataccgcct gcgctgtact gccagtttga cggtgcatcg cgacccggtt
      541 gttccggggc gtgcaggtac aggctcaccg ccacgcgcag gtagtagtaa aggccgcttg
      601 ccgaaccgac aaccacggcg cccaccagcc accacaagtg tgcctggaca ccgaccgcca
      661 gcacgtagaa cttaccaata aagcccagcg tcatcggaat accggccaga gacagcatca
      721 tcaccgtcat caccgccgcg aggatcgggc gatgccagaa cagtccgcgg taagagtaca
      781 gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg ccgaacgcgc
      841 cgaggctgct aaacagataa ccggccaggt aaacccctac cgcttccatc gacatctcgc
      901 cagtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata gatgagtaac
      961 cgagcagacg tttgatattg gtctggctca gcgccatcag gttaccgaag atgatggagg
     1021 caaaggcgat aatcgccagc accacgcgaa tcgcttcgct gtcacccatc ggtgcgtaga
     1081 ggaacagacg catcaccaca ccgaagatag cgattttgct cgccgtcgcc aagaaagtgg
     1141 aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac ggcaccagag
     1201 agagtttgaa gccgaggcca acaatcatca ggccgaaacc tgccagcaac agcggctcgt
     1261 tgagcatacc gtcgccaagg tttttaccca acgcgacaaa cgacaggtcg ccagactgcg
     1321 catacaccag cgccatacca aacagcagga aagaagacgc tgcggcagaa aggatggtgt
     1381 atttgatact ggcttccagt gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca
     1441 gcggcaaaga gatcagttcg ataccgagga acagagacgc cagatggttg gcattcgcca
     1501 gcaggatccc gcccagcgcg gcaattaaca ccaacaggta gaactcgtcc ttgttgtcgt
     1561 tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc aacaatacca
     1621 gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag cggcgtaacg tccatagcgc
     1681 ctgcctggcc gacaaaccag agcgaaacca gcgccgcgtt aagcccaata accgagagcg
     1741 tagcgttgag gaaatgattg cgtcgccacg caatggagag catcacaacc accaccgtca
     1801 agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt gtcatggcga
     1861 tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg ccaatcgcgg
     1921 agtgcgaggt atccagaatc ggctgcggat agaagccctg cagtaccagc agcaccacca
     1981 gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg ctggcaatct
     2041 cgcttttcgc tttaccgaag taagcgcgat gcaacatcgc cagcgaataa acagatgcaa
     2101 agaccagccc aaaggtagag ataacggtaa tcaccgggac aacctggaag ctgccgaaca
     2161 gaatcataaa ttcgccgacg aagttaccgg tgccaggcat cccaagcgtt gccaccgcaa
     2221 agaacagcga cagtgctggc agccatttca tcttgctcca cagaccgccc atcatgcgca
     2281 tgtcgcgggt atggatacgt tcataaagct gaccacaaag aataaacaga cccgccgccg
     2341 acaagccgtg cgcaatcatc tggattaccg cgccctggta ggccaactgg ctgccggtgt
     2401 agatagcaat cagcacgaag cccatgtggg aaaccgaggt gtaggcgatc agacgtttga
     2461 tatcggtctg ggcgaaggcc atccacgcac cgtagaagat gccgataaca cccagccaca
     2521 tagcaattgg cgcgaactct gccgacgcgt tcgggaacag cggcagggag aaacgcagca
     2581 aaccgtaagc ggcagttttc agcaagatcc ccgcgaggtc aacggaaccg gcggtcggag
     2641 cctgggagtg cgcatccggc agccagccat gcagcggaac caccggcatt ttgactgcga
     2701 aggcgatgaa gaagcccagc attaacaggt attccgcacc actggacatt ggcgtattca
     2761 gcagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg
     2821 ccaggatggc aatcaacatc accagaccac tcgcctgggt gtaaatgaag aacttggttg
     2881 ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc ccacagtgcg atcaggaagt
     2941 acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg atggcaagga
     3001 acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag ccctgatatt
     3061 tttcgatctc tttccacgaa catagtaccg ccagcacacc gagcagaccg gtcagcacga
     3121 ccatcagcag cgacagcccg tcaatggcga gatgaataga gatacccaaa cgcgggatcc
     3181 acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc agtgaataac
     3241 cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga
     3301 tccagcgtgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag ccgccaataa
     3361 agggaattaa tattagccag ggtagtaaca tggcgatctt tattccttgt aaaagtcccg
     3421 tcaggaccgg attttcaacg aattctcacg acagaaaatt ctcttcggac tgggggtagt
     3481 gtcggatgca ccgcgaaggc ggcttatccg acctacgttc ttatatgccc aggggcaatc
     3541 ccacaatcct taactcaacg cagtaccatc aacagtgcca gcaccacgac cgcaccgatg
     3601 ctcatggatg ccacatacca gcgcagatag ccgttctcac ttaacagcag acctttacct
     3661 gcaaagcggg aaaggacagc cggaatgttc atcattgagt tcagcggatc gcgtttcagc
     3721 aaccaggcaa tacccaggaa cggcctgacg aacactttgt cgtacagcca gtcaaagccc
     3781 caggcgttgt accaccaggt actcagcagg cggcccggcg cactgttggc gatggaggtc
     3841 accagagtac gtttacccag ccacagccaa gctgccagca gaatgccgac aatcgcgacc
     3901 acgccagagg taatttccag ggtcaacatg ctgccgtgcg ccagttccgt cgtttgcgga
     3961 agcacgccct gcagcggcgg tacaatcagt gcgccaacga aggtggaaag gatcagcagc
     4021 acaatcagcg gcaggctgtg agttacccct ttcacggcgt gagcgtgaat ttgttctttt
     4081 ccgtggaaga cgatgaaaat catacggaag gtgtagagcg aggtcataaa cgcaccgacc
     4141 agacctgcca ccatcagatt gatatgacca ttcgccatcg cacccgcgag gatctcatcc
     4201 ttactgaaga agcccgcagt gaccagcggc agtgccgaca gtgctgcgcc gcccaccagg
     4261 aagcagagat aaaccagcgg aatagattta cgcagaccgc ccatcttgaa gatgttctgt
     4321 tcgtgatggc aggccagaat gacggaaccg gatgccagga acagcagcgc tttaaagaac
     4381 gcgtgagtca tcaagtggaa aatcgccgca tcccatgcct gcacgccaag cgcgaggaac
     4441 atgtagccaa tctggctcat ggtagagtaa gcgagaacac gtttgatgtc ggtctgtacc
     4501 agcgcggcaa aaccggccag cagcagcgta accgccccga caatgcccac cagatgcaga
     4561 atttccggcg tcatcaggaa caggccgtgg gtacgggcga tcaggtagac acccgcggtt
     4621 accatggttg cggcgtggat cagcgcggag acaggcgtcg ggcccgccat cgcgtcggca
     4681 agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg caccgcccag cagcatcagc
     4741 gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg ctggtgccag ttccaccatt
     4801 tcgcggaagt tcagggtgcc cagttcgttg taaagaatga acagtgcgaa agcgaggaaa
     4861 acgtcaccca cacgggtcac gacgaacgct ttcattgccg ctgcgccatt cttcggatcg
     4921 gtgtaataga aaccgatcag cagataggag cacaggccca cgccttccca gccgaggtac
     4981 atcagcagca ggttgtcggc aagcaccaga actaccatgc tggcgatgaa caggttggtg
     5041 taggcgaaga agcgagagta gccctcttca ccgcgcatgt accaggaggc gtacatgtga
     5101 ataaggaaac ccacaccagt gaccaccgag agcatggtca gcgacaggcc gtccagcacc
     5161 aggttaaaac cgatgttaaa gtcgcctacc gacatccacg tccacagcgg ctggctgtat
     5221 gcctgctcgc cgttagcgaa gaaatcaacg ccgataaagg cggttaccag cgccgccagg
     5281 cccacagagc caacgccgac gatcgccgaa acgttttcag accagcgccc acgggagaac
     5341 gccagcagga cgaagccaat caatggcaaa ataatggtta aggcaagcat gttcatccgc
     5401 gcatctcact tactgaatcg atgttcaggt tctggcgacg acggtgaagt tgcagcagca
     5461 gcgcaaggcc gatactcgct tctgccgccg cgaggctgat ggcgagaatg tacatcacct
     5521 gaccgtcggt ctgaccccag tagcttccgg cgacaacaaa ggccagcgcg gaggcgttaa
     5581 tcatgatttc cagaccaatc agcataaaca gcagattgcg acggataacc agaccggtta
     5641 agccaagaat gaataagatt gccgcgagga tcagtccatg ttgtaagggg atcatgcgtg
     5701 ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg ctcagcactt cacccgcacg
     5761 ctcttcacga ccgacgtgga aggccacaac cagacctgcg agcagcagca tagaagccag
     5821 ttccaccgcc agtacgtaag gcccgaacag cgtaataccc actgctttag cactgattgg
     5881 cgtaccgtcg ataccctgat cgttaacacc gaggatggcg taaacaatca ccaccagcat
     5941 gatggccgac aaaattgccg gaccaatcca cacctgcggt ttcagccact ggcgttcctg
     6001 ttcgatttct gaaccgccca ggttgagcat catcaccacg aacacgaaca gcaccataat
     6061 ggcacccgcg tagacgataa tttccagcgc accggcgaag taagcgccca gtgagaagaa
     6121 caccccggaa atcgccagca gcgaaataat caggtacagc agtgcgtgta ccggattggt
     6181 atgggtgatc actcgcaagg tcgcaagtat ggctatcagg ccacagatat aaaaagcgaa
     6241 ctccattgcc cctctcctta cggtaacagg ctcttgacgt cgataggctt ggcttcgttc
     6301 tctgcttcgc ccttatcttt gccgtcgatt gccatacctg ccatccggta gaagttatat
     6361 tccgggtatt tgcccggacc ggagatcagc agatcctctt tttcgtaaac cagatcctgg
     6421 cgcttgtatt cccccatttc gaaatccggg gttaactgaa tcgccgtggt cggacaggct
     6481 tcttcgcaca gaccacagaa aatgcagcgt gagaagttga tgcggaaaaa ttccggatac
     6541 cagcgaccgt ctttggtttc tgctttttgc agcgagatac agccgaccgg gcaggctacc
     6601 gcgcagaggt tacaggctac gcaacgctct tcgccgtccg ggtcgcgggt cagaacgata
     6661 cgaccacgat aacggggcgg cagatagatc ggctcttccg ggtacattcg cgtttcgcgt
     6721 ttggcgaacg cgtgcaggcc gatcatccag atactacgaa cctgggtgcc gaaacctact
     6781 aacaattctt ttaaggtcat ggtcttattg ccccttattg cgcctgccag agaatgacag
     6841 ccgccgttac cagcaagttg atcagcgtca gcggcaggca gattttccag ccgaaggaca
     6901 ttacctggtc ataacgcgga cgcggtaacg acgcacgaat caaaatgaac atcatcataa
     6961 agaacgcggt tttcagcgcg aaccagatga atggcggtaa caacgggcct tgccagccac
     7021 cgaagaacag cgtcaccatc aatgcagaga tggtcacaat cccgatgtac tcacccacga
     7081 agaacagacc gaacttcata ccggaatatt caatgtggta accatccgcc agttcctgct
     7141 cggcttccgg ctggtcaaac gggtgacggt gacataccgc cacgcccgcg atggcaaagg
     7201 taataaaacc aaagaattgc gggataacgt tccacacatg cgcctggctg ttgacgatgt
     7261 cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat cagggaaagc ccgaggaaca
     7321 cttcgtagct cagggtctgc gcagaagcac gcatcgcacc cagcaacgag tatttgttgt
     7381 tacttgacca gcccgcaaac agcaccgcgt aaaccgccag acctgccatc atcaggaaga
     7441 acaaaatccc gatgttcagg tcggcaacca cccaacccgg actgactggc acaatcgcaa
     7501 aggccagcag cagcgaggta aaggcaatca tcggtgccag ggtaaagatg acgcgatccg
     7561 agaatttcgg gatccagtct tctttaaaga acattttgat catgtccgca accagctgga
     7621 gcgaaccgcc ccagccaaca cggttaggtc cgtaacggtt ctggaacaga cccagcaggc
     7681 gacgttcgcc aaagctcatg aatgccccgc aggtgacaac caccagcagg atcaccaccg
     7741 ctttgaggat ggtcagcagg atctcaatca gttccggtga tatccaactc attgttgtgc
     7801 ctccttgaga tcctcaagat gcgcgccagc cagcaccgga gcaataccgg acatacccat
     7861 cggcaagccc acctgccctg ccgtcagccc ttcagcgatt tcaaccggca gcgtgaccgt
     7921 attgccatcg taactaaagg agacgtgtgt acctgcgttc acacctaact tcgcggcatc
     7981 cgctgggttg agtttgatgt acggctgcgg catacggctc tggaagaccg gagcacgctg
     8041 tgacaattca tcgctgccaa acaggtggta atacggcgcg atacgccatt tcccgtcctg
     8101 cggctggaag cgtgccggta cgctggtgaa gtaatccaga ccattttcgc tggtttcaaa
     8161 cagacgcacg cccggatcgc caaagcgcag tttgccgccc acttcgtcct ggaatttgtt
     8221 ccacgcctgc ggggagttcc agcccggcgc ccaggcaaac ggcacttgcg aacggtgcgc
     8281 agtcggctgg ttgttacctt ccatcgagaa ggtgaacatg gtgtcaatat cctgcggctg
     8341 acgcggctca tgaacgctga tattggcgcg catggcggta cgaccgctgt aacggtgcgg
     8401 ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca tccggcgcag catctttgat
     8461 acctgccagt tccgggattt tcgccacaac agcgtcaatc acatggtcga gctgcgtcca
     8521 gtccacttca cggctcagca gggtgctgtg cagcgagtgt aaccagcgcc agctttccag
     8581 catgacagtt ttgctgtcgt aataagcagg atcgtaaacc tggaagaaac gttgggcgcg
     8641 gccttcgttg ttgatcaccg taccgtcgct ttcagcaaaa ctggcagcag aaagtaccag
     8701 atgggcgttt tccataatcg ctgtgcgttg atgatcaacc accatcacca gcggcgcttt
     8761 agccagcgca gcattcacgc gggtagcgga agcgtgacga tgcagatcgt tttccagcac
     8821 caccaccgcg tcggcgcgtc cggtttccag ttcggtcaac gcttcttcaa gcgaaccgcc
     8881 acccataatg cccagcccca tgctgttgac ggaacgggca atcatggtga taccaacgtc
     8941 agcgccgcga cctttcaggg ctttcgcgac gttagccgca gcctgaatca cctctatgct
     9001 accggcgttc gtcccggaga taatcaacgg tttcttcgca cctgccagtg cctgcacgat
     9061 gacgtcgatt ttgctttgca gctcaggttc gataccgtca accgctggcg cagagttatc
     9121 cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct tcaaccggtg cgcggtaagt
     9181 ccacgccgcg atatcatcca gacgggtgtc atcaacgttg gtaacaaaca gcggatgctt
     9241 cgcacgttga ccgatgttga ggattgccgc aatctgccag tcagccactt tctgtgctgc
     9301 cgccatttcg cgcgctttac ctttcacagc ctgacgcact gccagcgcga cgcgcgcgcc
     9361 ggtctgggta acgtcttcgc ccagcaccag taccgcatcg taagattcga tttcgcgcag
     9421 agccggagta taaatgccgc cttcacgcag cactttcagc gccagttgca gacgttcctg
     9481 ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc accagttcac gcagcgcaaa
     9541 gttgctttcc acgctggcac gcggagaacc aataccgatc actttcttcg actgacgcag
     9601 aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg gtaatgaaat catcgccacg
     9661 acgctgtact ggctgacgcg gacgatcctt caggttgacg taaccgtaac cgaaacgacc
     9721 acggtcgcag aggaagtagt ggtttaccgt accgttgtaa cggttttcga tacgacgcag
     9781 ttcgccgtaa cgttcaccgg ggctgatgtt acagccgatg gaacattgct ggcagatgct
     9841 cggcgcaaac tgcatatccc atttacggtt gtaacgctcg gagtgcgttt tgtcggtaaa
     9901 tacgccggtc gggcaaattt cgaccaggtt accagaaaat tcgctttcca gcgtgccgtc
     9961 ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa acgcccagat ctgtaccgtc
    10021 agcgtaatct ttgtagtaac gcacacagcg gtagcaggcg atgcagcggt tcatttcgtg
    10081 agagatgaat ggccccaaat cctgattacg gtgggtacgt ttggtgaaac ggtagcgacg
    10141 gaagctgtgt ccggtcatca cagtcatatc ctgaagatgg cagttaccgc cttcttcaca
    10201 taccggacag tcgtgcgggt ggttggtcat taaccactcg accacacttt cacggaactg
    10261 tttcgcttct tcgtcgtcaa tggaaataaa ggtgccatcg gaagccggtg tcatacagga
    10321 catcaccagg cgaccacgcg tgtcttccgc gttttggtat tgcttcaccg cacactggcg
    10381 gcaagcaccg acgcttccca gcgccggatg ccagcaaaag taaggaatat caaggcccag
    10441 agacagacaa gcttccagca ggttgtccgc tccgttgacc tcgtattctt tgccgtctac
    10501 atgaattgta gccattagca tgcttccagt tttctcggtc agggaccgag cgttaatcga
    10561 aattcggtta ccagcgctct ttcagcaggt tcggctgaat cccattaatc aaatgggtat
    10621 tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg gaaatatttg atggcgctct
    10681 gtaacggctc cactgcacca ggtgcgtggg cacagaaagt tttacccggg cctaagaatc
    10741 gacacagttg ctcaagtgtt tcgatatcgc ccggctggcc ttcgccacgc tccagcgcac
    10801 gcagaatttt cacgctccac ggcagaccgt cgcggcacgg cgtacaccag ccgcaggact
    10861 cacgggcgaa aaactcttcc aggttacgca ccagcgacac catgttgatc tcatggtcaa
    10921 ccgccatcgc cagcgccgta cccagacggc tgcccgcttt accgatactt tcgaattcca
    10981 tcggcagatc aaggtgcgct tcggtcagga agtcagtccc cgcgccgcct ggctgccaag
    11041 ctttaaattt cagaccatca cgcataccac cggcgtaatc ttcgaggatc tcgcgtgcgg
    11101 tggtgccgaa cggcagttcc cacagtcccg gatttttcac ccgaccggag aagcccatca
    11161 gcttggtgcc agcatcttta cttttcgaga tgttctgata ccactccacg ccgttagcga
    11221 ggatcgccgg aacgttacac agggtttcga cgttgttgac acaggtcggt ttgccccata
    11281 cgccggaggt tgccgggaag gggggtttcg agcgtgggtt agcacgacgc ccttccaggg
    11341 agttgattaa cgctgtttct tccccgcaga tgtagcgccc tgccccggta tggacgaaca
    11401 gttcgaagtc gaaacctgtt cccataatgt ttttgccaag caagcccgct tcagtggctt
    11461 cggcaatggc gcggcgcaga tttactgccg cttcgatata ttcgccacgc aggaagatgt
    11521 agccacggta agctttcagc gcaaacgcgg agatgagcat accttccacc agcaggtgcg
    11581 gcagttgctc catcaacagg cggtctttat aggtgcccgg ctccatttca tcggcgttac
    11641 acagcaggta acggatgttc atggattcgt ctttcggcat caggctccac ttcaggccag
    11701 tcgaaaagcc cgctccgccg cgccctttca gaccagcgtc ttttacctga ttaacgattt
    11761 cgtccggaga cagcccggtc agcgccttac gcgccccttc gtaaccgttt ttgctgcggt
    11821 attcgtccag ccacactggc tgtttgtcat cgcgcagacg ccaggtcagc ggatgcgttt
    11881 cgggagtacg gataatgttt ttcatttata ccgctccagc agttcaggga tcgcttccgg
    11941 ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt ggccctttat cacagttccc
    12001 caggcagcaa gttggcagca gcgtaaagcg gccatcaaat gtcgtttgcc ctggtttgat
    12061 gttcagcttt ttctcgagcg ccgcctgaat accctgatac ccgttgatat gacagaccac
    12121 gctgtcgcaa taacggatca catggcgacc aaccggctgg cggaagatct gactgtagaa
    12181 cgttgccaca ccttcgacgt cgcttgccgg aatacccagt acatcggcga tcgcgtggat
    12241 cgcaccatcc ggcacccagc cacgctgctt ctgaacgatt ttcagcgctt caatggacgc
    12301 cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc gcttcacgct ctgccgcact
    12361 cagctcaaaa gcctcggttt gtggttgttg attctcgtgc ataattagcg gtccacatct
    12421 gacataacaa aatcgatact gcccagataa acaatcaggt cagacaccag gctgccgcgg
    12481 atcgccgccg gaatttgctg caaatgcgca taactcggcg tgcggatacg agtgcgataa
    12541 ctcatggtgc taccgtcgct ggtcaggtag taactgttga tccccttggt cgcctcaatc
    12601 atctggaaag attcattggc aggcatcacc ggcccccacg acacttgcag gaagtgggtg
    12661 atcagggttt cgatatgttg cagcgtgcgc tctttcggcg gcggcgtggt cagcgggtga
    12721 tccgctttga acgggccttc cggcatgttg ttgaggcact gctcaagaat gcgcagactc
    12781 tggcgcagct cttccacttt cagcattacg cgggtgtagc agtcagaaac accacctcca
    12841 accgggattt caaagtcgaa gttttcatag ccagaataag gacgcgcctt acgcacgtcg
    12901 aagtcgatcc cggtagcacg caggcccgcg ccagtggtgc cccactccag cgcctctttc
    12961 gcgccatagg cggcaacgcc ctgggaacga cctttcagaa tggtattttg cagcgccgct
    13021 ttctcgtaag acgccagacg tttcggcatc cagtcgagga actcacgcag caggcgatcc
    13081 cagccgcgcg gcaggtcgtg cgctacgccg ccaatacgga accacgccgg gtgcatacgg
    13141 aaacccgtga tcgcttccac cagatcgtaa attttctgac gatcggtaaa ggcgaagaac
    13201 acgggcgtca ttgcgccgac gtcctgaata aaggtcgaga tgtacagcag gtggctgttg
    13261 atacggaaca gttcggagag cataacgcga atgacgttaa cgcgatccgg cacggtgatc
    13321 ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt taacgcagcc gccgaggtat
    13381 tcaatacggt cggtatacgg aatgtagctg tgccaggact ggcgctcgcc cattttctcc
    13441 gcaccacggt ggtggtagcc gatgtctggt acgcagtcga caatctcttc gccgtcgagt
    13501 tgtaaaacga tacggaaagc cccgtgcgcc gacgggtggt tcggaccgag gttgaggaac
    13561 atgaagtcct cgttttcggt gccgcgcttc atcccccact cttccggttt gaaggtcagg
    13621 gcttccatct ccagatcctg tttggctttg gtcagctcaa acggcgagaa ttcggtagcg
    13681 cgcgccgggt aatctttacg cagcgggtga cctttccagg tttgcggcat catgatgcgg
    13741 cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat cccaggtttc acgctcatac
    13801 cagttagcgt tcgggaacag tttggtgaag gtcggtacgt gcaggtcgtt ttctgccagc
    13861 gccaccttca gcatgatgtc gcggttacga tcgatagaaa tcagatggta gaaaacggaa
    13921 aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac gttcgtccat gccgtgtaag
    13981 tcaaacagca tgacgtaagg tttcggcagt ttctttaaga aatcgccaac ttccagtaat
    14041 tgttcacgct tgatccacac aacgggaacc ccggtgcgag tcgcctgaac agtaaaggca
    14101 tccggcccaa aacggttgcg cagttcgcca atcaccggat catcaagatg atcacgggtc
    14161 tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt tcaccattgc aaatggtccg
    14221 tggtgactgt cggcgcagta agcttcgcga aatagattta ggaataagcg aagaaaaatc
    14281 cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac gcaggttggt tacggcaatg
    14341 cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg cgcgataaac gccctgatcg
    14401 ccaaccaccc aggagagcgg acgacgttct ttgccgatag attcctgcaa cagcatcagt
    14461 gcctgcatgt acgcttcagg acgcggcggg cagcccggga tatacacatc aaccgggatg
    14521 aatttatcga cgccctgcac aacggaataa atatcgtaca taccaccaga gttggcacag
    14581 gcacccattg agataaccca ttttggttcc agcatctggt catacagacg ctgaataacc
    14641 ggtgccattt tggtaaagca ggttcctgca accaccatca ggtcagcctg acgcggcgaa
    14701 gcacgcaata cttctgcgcc aaaacgcgcc acgtcatgca ccgcggtaaa cgaagtcacc
    14761 atctcaacgt aacagcagga aagaccgaag ttatacggcc aaattgagtt tttacgaccc
    14821 cagttaacca tgtcattgag cttgcccata aacacgtttt tgttaacttc ttgctccaga
    14881 gggtcggtta cgatctcctg cttttgcagg gggtaacggt cgttctcacc gttgggatct
    14941 atgcgggtga gcgtataatc catcttaatg cctcgcggtt agcgttgacg attagcgata
    15001 ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg cgggcgtcca gtccagcgcg
    15061 ccaatacgca ccagataaac cagacctgcc agtaacacaa aaataaaaat tgcagcttcc
    15121 acaaagccta cccagccgct ttcgcggata gaggttgacc atgcgaacag atacagcgct
    15181 tcaacgtcga agataacgaa gaacatggcc accagataaa acttggcgga caggcgtaag
    15241 cgggcggagc cgaccgagtc gataccggat tcaaacggca cgtttttcga cctcgcgcgt
    15301 gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc aacacaggcc aatggcaacg
    15361 ataagaaaga tagcgaatgc ccagtgatga gcgatgactt cagtggatgt tgacatactc
    15421 attgcttact catcaaaagt agcgccagat tcactgctct tcacggcaga tggacgccac
    15481 atcgattcat ggggaggaat aaaaaaaacc ttacaatcac tgtagaaatt cttttataca
    15541 gctaattgat gtggtctttt actcctttct ataacctttt gtcaacttta acaaaagttt
    15601 cttcacatta gtttacataa tatcaacacc attagcattt aatgcccttt cacgccagat
    15661 ccttgacgac tccaggataa ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa
    15721 aaaatacggg tctattttga caggaatttg tgtcgattcc tccccccaaa agagagtatt
    15781 ttcttgatct gtgacacgct tttgtcattc cataacaaaa acgcagcaac aaatttacgc
    15841 atttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gttcgggacg
    15901 tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt ctgcactatg cgaggaagga
    15961 tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt ttttacacta
    16021 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    16081 atcatcccct tccggagcag acattgggct gtattgccac ggattatggt agctctccat
    16141 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    16201 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca ggtacgggct
    16261 catcatttca atcggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    16321 cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat taagcgtcgc
    16381 cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcgcc
    16441 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa
    16501 tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    16561 catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac
    16621 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    16681 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    16741 acgcagtatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    16801 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    16861 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    16921 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc
    16981 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    17041 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    17101 aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc
    17161 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    17221 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    17281 gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    17341 agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggtc
    17401 attgcgaatt atctgatgca aagttatgtt aggcaaagta atcttctatt attaataagc
    17461 acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc attcattaat
    17521 tccgcttaac aatagtttca caccttgccc ccattgcgac acctcggaaa tcatcacgct
    17581 gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa ttcttaaatg
    17641 aggcattttc acacaattat cttacagata aaaaaaccag acttgcaatt aagaatcaga
    17701 acaattcact atataacatt gcatgcaaag catatacgcc tcattatttt gtcattatta
    17761 agttattaac agcacaatcg agccttcccc tctggcaaaa tcttattctg cagaccttca
    17821 aaacaccgtc ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat
    17881 aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg
    17941 tctgttatga catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta
    18001 acaaggtact gaaactgaac atcggcaacc cagccccatt cggttttgac gcgccagatg
    18061 aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca agggtattgc gattccaaag
    18121 ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg
    18181 ttaccgtgga agatatttac atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc
    18241 aggcattgct gaacagcggg gacgaaatgt tggttcctgc accagattac ccactctgga
    18301 ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg
    18361 actggttccc ggacctcgat gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg
    18421 ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga
    18481 ttgtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa
    18541 ttctctacga cgatgctgag catcactcaa ttgcgccact ggcacctgac ctgctgacca
    18601 ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg
    18661 tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt
    18721 caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt
    18781 atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg
    18841 cgtgggaact gatcaacgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt
    18901 atatgttccc gaaaatcgac gccaaacgct ttaacattca cgacgatcag aaaatggtgc
    18961 tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc aactggccgt
    19021 ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga
    19081 gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga
    19141 gattgcatcc ggcagcgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt
    19201 cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg
    19261 gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat
    19321 gctcgcccat gcgctggcag ctatcaaaaa tcgaaaattt ggcggtaatg tcaacgccga
    19381 acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc
    19441 tactccggtg aaatacttca attcgcaaat cgctcaggag tacaaggcta ttgaaaaaat
    19501 cgctcagcaa aaactggtca atatggttcc ggaagagctg cgggatatct ttgcgccgtt
    19561 aattgacgag catgcatata gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact
    19621 gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga aataatgaat tcttgctggc
    19681 aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc caggagatgg actacttcat
    19741 ggaagtattt gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct
    19801 gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc cggatgcgac atcaggctct
    19861 tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg
    19921 aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagt
    19981 gtgttaacag gtgaagaaac cggtgtcata aaggcggcgg atgctgccat cgccacgacc
    20041 atcgcgaatg gataaggcga cacccccatc gttttggcag cagccagcgc aatcggagcc
    20101 atcaacaccg ccgtcgcggt attagagata aatagcccaa taaccgccga caagacaaac
    20161 aaacacccca gcatcatatg tggcccgtaa ccaccgccaa tgtccatcag ccctttcacc
    20221 gccagcgcga cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc
    20281 aaaataatgc tcggccagtg aatggattta taggcgcttt cagcatctat acagcggaat
    20341 ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc atctgtcagc
    20401 attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctggct gtgcgcgggc
    20461 gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc
    20521 agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc
    20581 tcatccgcca gcgaaccttc cagcgccacg ccattgcgct ttagccccac cacattcagt
    20641 ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caatcagttc tgactcagga
    20701 attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc
    20761 agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca
    20821 gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg
    20881 ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcgcc ataacgctca
    20941 cgtaatttga gatcatccag ccgttgacca atcattggcg atccggggcg aatagccaga
    21001 cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt tcgacgcgtc
    21061 cagccttcgc gctgcggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata
    21121 tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag
    21181 ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc
    21241 agcgtcatca tgccgcttat cagcccggca aaactcaacg gcatcatcag acgtgacggc
    21301 gaagtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgccg
    21361 gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg
    21421 atttcactgt tgcccgcaac tttgaccagc catgttccca ttacggtggc aacaccggta
    21481 cggaccaaac catcgccaat aataaacaag gcggcaatca ggacaacgtt aggatcagaa
    21541 aagccggaaa atacttcggg gactgtcagc gttccgctta atgcaaacgc gacaataaca
    21601 aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacaat ggcaacggcc
    21661 agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta attgagggag
    21721 atgtcttgat tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt
    21781 ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt
    21841 ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga
    21901 catgacaacc cgccgccagg ccagaaagca cgccagcgga gcatcttcca ccaccacaca
    21961 ctcctgcggc gcaagcccca gcagctgcgc gcctaacaga tacgcatcag gttctggttt
    22021 tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc cagctatttt
    22081 atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc
    22141 tttattcaaa tgactgagta aggcgattgc ccccggaagc gggtaatacc ttcggtttcc
    22201 gtggcctcga tgtgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc
    22261 gccataaaat ggcgcagaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct
    22321 tccggcgcta acccatgacg tctggcccag ttgctccacg cccgttctac cgcaggcagg
    22381 gaatccacca gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc
    22441 tcaggcattg ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc
    22501 atgccacaca ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc
    22561 gtgagttccg gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata
    22621 accgaggtaa cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc
    22681 gatggattgc tggtcctgca aattagacca ggaaacatgc aacgcatgat acatctccat
    22741 aatgtcgatg atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccaattc
    22801 acgcatctgt aatccgtaac ctcgctcaat aattgtttgc aggcgacggt aacgttcagc
    22861 atttgccgga tcgagcatag tcatcatctt gtactgatta gacaaaataa gacgttgcgc
    22921 gttggtcatt tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct
    22981 gagtatgcct tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt
    23041 tatccagttg tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc
    23101 cttcaacggg tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc
    23161 agttaaggat tgcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct
    23221 gcatgggtaa acttaaggcg aacagtgcag tggcaactgc cggcccaagc tgaccgccca
    23281 gggcaatctg ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac
    23341 gcgtcatctt gataacgcga ttttcgacaa agaccggggc aaggcgtttt tccagtggcc
    23401 acgtctttga gtaatgctgt ccgcggcgaa acaagctaaa aaaattaaca gaacgattat
    23461 ccggcgttga catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt
    23521 cacaactcag cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc
    23581 tttagcagcc tgaaggccta agtagtacat attcattgag tcgtcaaatt catatacatt
    23641 atgccattgg ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc
    23701 aaaaaaaatt tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct
    23761 ctatggctcc ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta
    23821 agttagtact ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag
    23881 taaatggtga agagtacctt tctggtttag ccgaatgttt ccacctgcct gaagcacgta
    23941 tcaaatggaa aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca
    24001 gcgaagcgct caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc
    24061 agctgactgc tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag
    24121 tgatcgatga gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca
    24181 acccggctca cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca
    24241 aaaacgttgc tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct
    24301 acgccctgcc ttacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca
    24361 ccagccactt ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac
    24421 tgaacatcat cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta
    24481 aatgcgttga cacctctatg ggcctgaccc cgctggaagg tctggtcatg ggtacccgtt
    24541 ctggtgatat cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg
    24601 acgcaatcaa caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca
    24661 gcgactgccg ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg
    24721 acgtttactg ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc
    24781 gtctggacgc tgttgtattc actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac
    24841 tgtctctggg caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg
    24901 cacgtttcgg caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc
    24961 caaccaacga agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca
    25021 ccgccagctc agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg
    25081 ataaaccgtg tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag
    25141 cgtcagcctt ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa
    25201 acctatcgct cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg
    25261 tgcgaactct tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct
    25321 gctttccagc aatcagaaag atgtgctgat ggaagagatc gtcgcaaact accacgctaa
    25381 caccaaagac gctgaagtcg ttctggttga aggtctggtc ccgacacgta agcaccagtt
    25441 tgcccagtct ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat
    25501 gtctcagggc actgacaccc cggaacagct gaaagagcgt atcgaactga cccgcaacag
    25561 cttcggcggt gccaaaaaca ccaacatcac cggcgttatc gttaacaaac tgaacgcacc
    25621 ggttgatgaa cagggtcgta ctcgcccgga tctgtccgag attttcgacg actcttccaa
    25681 agctaaagta aacaatgttg atccggcgaa gctgcaagaa tccagccctc tgccggttct
    25741 cggcgctgtg ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca
    25801 cctgaatgcg accatcatca acgaaggcga catcaatact cgccgcgtta aatccgtcac
    25861 tttctgcgca cgcagcattc cgcacatgct ggagcacttc cgtgccggtt ctctgctggt
    25921 gacttccgca gaccgtcctg acgtgctggt ggccgcttgc ctggcagcca tgaacggcgt
    25981 agaaatcggt gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact
    26041 gtgcgaacgt gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca
    26101 gacctctctg agcctgcaga gcttcaacct ggaagttccg gttgacgatc acgaacgtat
    26161 cgagaaagtt caggaatacg ttgctaacta catcaacgct gactggatcg aatctctgac
    26221 tgccacttct gagcgcagcc gtcgtctgtc tccgcctgcg ttccgttatc agctgactga
    26281 acttgcgcgc aaagcgggca aacgtatcgt actgccggaa ggtgacgaac cgcgtaccgt
    26341 taaagcagcc gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc
    26401 ggcagagatc aaccgtgttg cagcgtctca gggtgtagaa ctgggtgcag ggattgaaat
    26461 cgttgatcca gaagtggttc gcgaaagcta tgttggtcgt ctggtcgaac tgcgtaagaa
    26521 caaaggcatg accgaaaccg ttgcccgcga acagctggaa gacaacgtgg tgctcggtac
    26581 gctgatgctg gaacaggatg aagttgatgg tctggtttcc ggtgctgttc acactaccgc
    26641 aaacaccatc cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc
    26701 ttccgtgttc ttcatgctgc tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa
    26761 cccggatccg accgctgaac agctggcaga aatcgcgatt cagtccgctg attccgctgc
    26821 ggccttcggt atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc
    26881 aggtagcgac gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga
    26941 cctgatgatc gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc
    27001 caaagcgccg aactctccgg ttgcaggtcg cgctaccgtg tttatcttcc cggatctgaa
    27061 caccggtaac accacctaca aagcggtaca gcgttctgcc gacctgatct ccatcgggcc
    27121 gatgctgcag ggtatgcgca agccggttaa cgacctgtcc cgtggcgcac tggttgacga
    27181 tatcgtctac accatcgcgc tgactgcgat tcagtctgca cagcagcagt aacctcgtca
    27241 tcatccgcag ctttgcgctg cggatatctg aaccggaaat aatcactatt tccggttttt
    27301 tattctctta atctgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg
    27361 aattgcgcca tttcacttta catacttacc actttgtttt gtgcaaggga atatttgcgc
    27421 tatgtccgca atcactgaat ccaaaccaac aagaagatgg gcaatgcccg atacgttggt
    27481 gattatcttt tttgttgcta ttttaaccag ccttgccacc tgggtagttc cggtggggat
    27541 gtttgacagt caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt
    27601 agatccacac tcatttcgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt
    27661 acagctgttc acgacgggcg atgaacgccc gggcctgatg aacttcccgt ttgaaggatt
    27721 aacctcagga tcgaaatacg ggacagctgt tggcatcatc atgtttatgc tggtgattgg
    27781 cggcgcgttt ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat
    27841 tcgccatacc cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact
    27901 tggcggcgcg gtatttggta tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc
    27961 gctaatggtc cggctgggct atgacagtat taccaccgtc ctggtgacct atattgccac
    28021 gcaaatcggt tttgccagtt cgtggatgaa cccgttttgt gtggtcgttg ctcaggggat
    28081 tgccggcgtt ccggtgcttt ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac
    28141 tatgattggc ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct
    28201 gtcacgcgtg catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg
    28261 tccgtttacc tttggtgact ggctggtatt gattgtcctg accgccgtaa tggtctgggt
    28321 gatttggggc gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac
    28381 catgggtctg gtgattggca tcatcggcgt tgttttccgc cttaacggca tgacggttaa
    28441 taccatggct tcatccttta ccgaagggac gcgaatgatg atcgcccctg ccctgctggt
    28501 gggtttcgcc aaagggattt tgctgctggt cggtaatggt gaagcgggtg atgccagcgt
    28561 gttaaatacc atcctcaaca gcattgccaa tgccattagc ggtctggaca acgcggtcgc
    28621 ggcctggttt atgttgctct tccaggcggt atttaatttc ttcgtgacgt ccggttctgg
    28681 tcaggcggcg ttaaccatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg
    28741 tcaggttacc gtgctggcat tccagtttgg tgatggcttc agtcacatca tttacccaac
    28801 ctcagcttcg ttaatggcaa ctctcggtgt gtgcagggtg gacttccgta actggctgaa
    28861 ggtgggcgcg accctacttg gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc
    28921 tcagttgatg ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc
    28981 tgccgtttca gtctctacgg cttcattttt ggcattgcgc ttcatccaca acgccagcgc
    29041 tttcagcgag tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca
    29101 gcaaacttca ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcagctgaa
    29161 caacgcgccc cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa
    29221 ggggacaccg gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg
    29281 ctcatcggcc tgaaccacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt
    29341 tgtctcggta cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt
    29401 tgcacgatga cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc
    29461 gttctcttca ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat
    29521 caggaaacct tctttttctg gcgctcttac ggcgcatttt gagttgtggg taaattacgg
    29581 attaatcgcg acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc
    29641 attatccagc atgccataac tcgccggatt accgcctttc ggaatactca ccgagccggg
    29701 gttgaagtgg aaaatctccc cccgctgttc tgccactggc agatgggtat gaccgtacac
    29761 cagcacatcg ttctggttta aagcaggtag attttccggg ccaaaaagat gaccatgcgt
    29821 caaaaacaga cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg
    29881 cagcagcatt tgatccactt cgctgtcgca gttgccgcgc acagcgataa ccttatgtgc
    29941 cacttcatta agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg
    30001 cgggccgtga ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttgggcaaa
    30061 caactccaga acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag
    30121 tttcatcact cactcctcgt cgaaaaaacg taagctatga tactggattc tgtcgttgct
    30181 atcagccaga ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct cgctgccact
    30241 ggaatgtcgc ataatccacc aggcgttctg gcacctcatc gccatgtagt accaggcggt
    30301 taatcattag cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggctgac
    30361 ccagtgctaa caaatgttct gccatcgcga acagtttctc tgcactggct tttccctcgg
    30421 ccgttagtgg cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga
    30481 tgggcatcag atcgctgcgc agccaggcct gaatccgtcg tgcacgcgca cgattttcta
    30541 aatcaagcgg ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg
    30601 caatggcaga agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg
    30661 tctgaccgta accttgccac gtcggcttca aatgttcacc gctgtcgagg tcgatggtct
    30721 tgatatgaaa cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg
    30781 agaaaaagtg ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa
    30841 taaagggggc tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc
    30901 acgtaagtcg ccgcgctttc tatacttgtg gtgttcagat gattaaacaa ccggagctgc
    30961 aatgatcgat ctctatttcg ccccgacacc caatggtcac aaaattacgc tgtttctcga
    31021 agaagcagag cttgattatc gcttgattaa ggtagacctg gggaaaggcg gtcagtttcg
    31081 tccggaattt ttgcgcattt cgcccaacaa caaaattccg gcaattgttg atcattctcc
    31141 tgccgatggc ggcgaaccgc taagcctctt tgagtctggt gccattttgt tgtatctgac
    31201 tgagaaaaca ggactctttt tgagtcatga aacgcgtgag cgcgccgcca cattacagtg
    31261 gttattctgg caggtaggcg gactggggcc gatgcttggg caaaatcatc attttaatca
    31321 cgcagccccc caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct
    31381 ttaccatgta ctaaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agaactacag
    31441 cattgcggat attgcctgct ggccgtgggt taatgcctgg actcgccagc gaattgacct
    31501 cgcaatgtat ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg
    31561 gcaggcactg ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt
    31621 tctcgtgtat tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc
    31681 gctattattc gtataaagaa ccttcgttta cgtacgttta tcggaattaa ggaagaagaa
    31741 attaacaacc gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg
    31801 cgcactagcg aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt
    31861 cagcatgtag agaataaccg tttctctttg ttggaaaaat taactcagga tgtgctcgat
    31921 atcgcacgtg aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg
    31981 ctgcgctacg ccgattcggt atccatgacc ttaagctggc agcgttaacc gccatatcgg
    32041 gaggctgcat gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc
    32101 cgcgtttgct ggagctgggc catcaaatca cggtagtgac gcgtaaccca cagaaagcca
    32161 gttccgttct cggccctcgg gtgacgctat ggcaagggct agccgatcaa agcaacctca
    32221 acggcgttga tgcggtaatc aacctggccg gagaaccgat tgctgataaa cgctggactc
    32281 acgagcaaaa agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa ctggtcgatt
    32341 tgattaatgc cagcgacacg ccgccgtcgg tattcatttc cggctcggca acgggctatt
    32401 atggcgactt aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttactc
    32461 ataaactctg cgcccgctgg gaagagattg cctgccgggc gcaaagtgac aaaacgcgag
    32521 tgtgcctgct gcgaaccggc gtggtactgg caccggacgg cggtattctc ggtaaaatgc
    32581 tgccaccgtt tcgtcttggc ctgggcgggc cgattggttc cggtcggcag tatctggcct
    32641 ggattcatat tgatgatatg gtcaacggca ttctctggct gctggataac gacctgcgcg
    32701 ggccatttaa tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg
    32761 gtcaagcgct gcatcgcccg gccattttgc gcgtccccgc aaccgccatt cggctgttaa
    32821 tgggcgaatc ttcaatactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag
    32881 aagcgggttt tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct
    32941 gatgtggttt acagcaaaca tccgccagtt aactcccggt gttacaggat tagtggcttt
    33001 gcgcgataag atcgtctggt gaaagtcggg tcaccatcat aactaactct ctgtctaaac
    33061 ctctatccag catctcctga gcaatacgca gggcttcttc gtgtttgccc tgcattgcgc
    33121 cttcttcacg taatctgtca gcaatggtca tcagtttctc cttttcttgt ggtgcgcgtt
    33181 ctgttatctc accaataaat gcacggaaac gctgggcatc ccctgtttgt aatacgtaat
    33241 taaacagggc ttttagctgt ctgtcattag tgttccctgt aactagcagc gaaacaattt
    33301 ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg
    33361 ccattttgcg gtgttgcata atctcgtcat ccggcaccac ggtaatatcc accaacggaa
    33421 acgctgatga atatattttg cgggctatag caggctcggc aaattcatca agccagcaga
    33481 gtgaataagg ataaggactt ctgcaaccat gataaaacag catcgggatc accaatggaa
    33541 gctctttata gcccgcatca agatggtttt gcattgccgc aatggaataa cgcatcatgc
    33601 gaaaagccat taattcttcc agcttacttt ggtgctctat cactacataa atataaccca
    33661 ctccctcctg cgttttcaca gaccacaaga ggtcggaata atattgccgc aggtcttcat
    33721 caataaaact gtttgattct agtttaagcg tcgttaaatc acacagtttg cgcagcggcg
    33781 cgggaagatg aatatcaata aaatcccgcg cggtgt
//
LOCUS       DAJTVE010000034        33248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000034 DAJTVE010000000
VERSION     DAJTVE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..665
                     /locus_tag="OO439_001533"
     CDS             <1..665
                     /locus_tag="OO439_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6315924.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            735..1511
                     /gene="yaaA"
                     /locus_tag="OO439_001534"
     CDS             735..1511
                     /gene="yaaA"
                     /locus_tag="OO439_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6315925.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1664..1960)
                     /locus_tag="OO439_001535"
     CDS             complement(1664..1960)
                     /locus_tag="OO439_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6315926.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2174..3460)
                     /gene="thrC"
                     /locus_tag="OO439_001536"
     CDS             complement(2174..3460)
                     /gene="thrC"
                     /locus_tag="OO439_001536"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6315927.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3461..4393)
                     /gene="thrB"
                     /locus_tag="OO439_001537"
     CDS             complement(3461..4393)
                     /gene="thrB"
                     /locus_tag="OO439_001537"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6315928.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4395..6857)
                     /gene="thrA"
                     /locus_tag="OO439_001538"
     CDS             complement(4395..6857)
                     /gene="thrA"
                     /locus_tag="OO439_001538"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6315929.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6884..7003)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6939..7004)
                     /gene="thrL"
                     /locus_tag="OO439_001539"
     CDS             complement(6939..7004)
                     /gene="thrL"
                     /locus_tag="OO439_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6315930.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7218..7904)
                     /locus_tag="OO439_001540"
     CDS             complement(7218..7904)
                     /locus_tag="OO439_001540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6315931.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8304..8444)
                     /locus_tag="OO439_001541"
     CDS             complement(8304..8444)
                     /locus_tag="OO439_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315932.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8540..9256
                     /gene="arcA"
                     /locus_tag="OO439_001542"
     CDS             8540..9256
                     /gene="arcA"
                     /locus_tag="OO439_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6315933.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9316..10668)
                     /gene="creD"
                     /locus_tag="OO439_001543"
     CDS             complement(9316..10668)
                     /gene="creD"
                     /locus_tag="OO439_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6315934.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10726..12150)
                     /gene="creC"
                     /locus_tag="OO439_001544"
     CDS             complement(10726..12150)
                     /gene="creC"
                     /locus_tag="OO439_001544"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6315935.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12150..12839)
                     /gene="creB"
                     /locus_tag="OO439_001545"
     CDS             complement(12150..12839)
                     /gene="creB"
                     /locus_tag="OO439_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6315936.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12852..13325)
                     /gene="creA"
                     /locus_tag="OO439_001546"
     CDS             complement(12852..13325)
                     /gene="creA"
                     /locus_tag="OO439_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6315937.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13536..14405
                     /gene="robA"
                     /locus_tag="OO439_001547"
     CDS             13536..14405
                     /gene="robA"
                     /locus_tag="OO439_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6315938.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14402..15049)
                     /gene="gpmB"
                     /locus_tag="OO439_001548"
     CDS             complement(14402..15049)
                     /gene="gpmB"
                     /locus_tag="OO439_001548"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6315939.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15101..15622
                     /gene="yjjX"
                     /locus_tag="OO439_001549"
     CDS             15101..15622
                     /gene="yjjX"
                     /locus_tag="OO439_001549"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6315940.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15707..16033)
                     /gene="trpR"
                     /locus_tag="OO439_001550"
     CDS             complement(15707..16033)
                     /gene="trpR"
                     /locus_tag="OO439_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6315941.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16123..18060)
                     /gene="sltY"
                     /locus_tag="OO439_001551"
     CDS             complement(16123..18060)
                     /gene="sltY"
                     /locus_tag="OO439_001551"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6315942.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18271..19938
                     /gene="ettA"
                     /locus_tag="OO439_001552"
     CDS             18271..19938
                     /gene="ettA"
                     /locus_tag="OO439_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6315943.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20245..21477)
                     /gene="nadR"
                     /locus_tag="OO439_001553"
     CDS             complement(20245..21477)
                     /gene="nadR"
                     /locus_tag="OO439_001553"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6315944.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21498..22880)
                     /gene="radA"
                     /locus_tag="OO439_001554"
     CDS             complement(21498..22880)
                     /gene="radA"
                     /locus_tag="OO439_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6315945.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22929..23897)
                     /gene="serB"
                     /locus_tag="OO439_001555"
     CDS             complement(22929..23897)
                     /gene="serB"
                     /locus_tag="OO439_001555"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6315946.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24003..24647
                     /locus_tag="OO439_001556"
     CDS             24003..24647
                     /locus_tag="OO439_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6315947.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24675..25691
                     /gene="lplA"
                     /locus_tag="OO439_001557"
     CDS             24675..25691
                     /gene="lplA"
                     /locus_tag="OO439_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6315948.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25723..25872
                     /locus_tag="OO439_001558"
     CDS             25723..25872
                     /locus_tag="OO439_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315949.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26147..26866)
                     /gene="deoD"
                     /locus_tag="OO439_001559"
     CDS             complement(26147..26866)
                     /gene="deoD"
                     /locus_tag="OO439_001559"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6315950.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26946..28169)
                     /gene="deoB"
                     /locus_tag="OO439_001560"
     CDS             complement(26946..28169)
                     /gene="deoB"
                     /locus_tag="OO439_001560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6315951.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28221..29543)
                     /gene="deoA"
                     /locus_tag="OO439_001561"
     CDS             complement(28221..29543)
                     /gene="deoA"
                     /locus_tag="OO439_001561"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6315952.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29670..30449)
                     /gene="deoC"
                     /locus_tag="OO439_001562"
     CDS             complement(29670..30449)
                     /gene="deoC"
                     /locus_tag="OO439_001562"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6315953.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30707..32257
                     /locus_tag="OO439_001563"
     CDS             30707..32257
                     /locus_tag="OO439_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6315954.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32229..33092
                     /locus_tag="OO439_001564"
     CDS             32229..33092
                     /locus_tag="OO439_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6315955.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7906 a         9123 c         8376 g         7843 t
ORIGIN      
        1 taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg
       61 gttcgacgcc aaacgcggca gcggcagcgg cgtcctggcc tccgcatccg gcagcgcaag
      121 ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca
      181 tgctgatatt actggcgggt aacgacacaa cctacatgcc gctggaaggt attcagctta
      241 tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc tgagtttgtt accctcgtgg
      301 ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa aacaatctct
      361 tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc accttcgcaa
      421 cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg gcaactggca gatatcataa
      481 tggcctgcat ggctattacc aatttgaccg ccattttact gctctcgcct gtggttcata
      541 ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt
      601 tgcgttatcc ggatatcggt cgccagcttt ctccggacgc gtgggatgat gtttcgcagg
      661 agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc
      721 tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc
      781 cgttgaccac cacgcgctat acgctgccgg agctgttaga caattcccag cacttgatcc
      841 atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgcatc agcgacaaac
      901 tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcacg ccggagaatg
      961 cccgccaggc tattttggcg tttaaaggtg atgtctacac cggcttgcag gccgaaactt
     1021 tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg
     1081 gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg
     1141 agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga
     1201 acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca gatgagtatt
     1261 ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg ttcctcgatg
     1321 agaagaacgg caaatttaag ataatcagct tctacgctaa gaaagcacgc ggtctgatga
     1381 gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt tttaatagcg
     1441 aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt aaacgctacg
     1501 agcagcgtta agtttgcagt caatgccgga tgcggcgtga acgccttatc cggcctacaa
     1561 aagcatgcag attcaatata ttgcagaatt cacgtaggcc tgataagacg cgccagcgtc
     1621 gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc
     1681 ctggaccatg accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg
     1741 gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt
     1801 ggtcgcgcca gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta
     1861 attttactga cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa
     1921 ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt
     1981 gcccgttatg ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc
     2041 aatcctaatc tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt
     2101 ctccataatt tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga
     2161 taatgaatag attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg
     2221 gcagattatg tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca
     2281 acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga
     2341 ggaacaagcc atattcgcct ggattcaact ggtcacacag cgcacgatac gctacggcag
     2401 cgtgcggctc cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt
     2461 catcatccac ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact
     2521 cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct
     2581 gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt
     2641 tggtcgcagc aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg
     2701 tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgcg
     2761 gcagctgcgc aactgcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa
     2821 tcgagttagc cgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct
     2881 tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc
     2941 ccaacgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa
     3001 ccactttcac attcggcaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg
     3061 aggtcgcggt cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca
     3121 taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac
     3181 cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct
     3241 cttccaggat ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc
     3301 gggtgacaaa atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt
     3361 catgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc
     3421 tgacctgctc gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg
     3481 cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct
     3541 tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg
     3601 tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg
     3661 cctgccggaa gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca
     3721 gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc
     3781 gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg
     3841 ccgtcgagac tttaattccc ggatacgcca gcacccacag ccactcatca aaccctggca
     3901 cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg
     3961 gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc ttccagctcg cccatcaaag
     4021 ccagcaaacg ggtgtcatta agtggcttgc cgcagtgttc attcatcgcc atcagcgccg
     4081 cgaccaccga acaggcgctg gagcctaagc ccgagccgat cggcatattc ttttccagag
     4141 tcatcgccac tggaatttgc ttgcccagct ccaggcaaaa acgctcccag cactgataaa
     4201 cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac
     4261 tgaatgtctc tgccgcctca accgtgacta catctccgag caatgcacca tcaacgggtg
     4321 tcaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat
     4381 aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag
     4441 acaccagcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc
     4501 tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga
     4561 tcattcccat ccacttcggc aatcttcacg cggcagacac catcttcatc aatattgcca
     4621 acatagcgca aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg
     4681 tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcggcgtt aaactctgcg
     4741 ggcagcacag gttcaatttc aatatccgcc agttccagtt cacgtcccgt ttcacgagcg
     4801 agaatcaata gtttacgcgc cacatccata ccagaaagat catctcgcgg gtccggttcg
     4861 gtataaccca tttcccgcgc cagcgtggtc gcctcggaga aactcatgcc ttcgtctaac
     4921 ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcac caattcatca
     4981 cctgcattga gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg
     5041 tcatagagga atttacgccg cgatttttcc gccgcataac gcaactgatg atagtaatcc
     5101 atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaggaag
     5161 tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc
     5221 agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgt
     5281 gcaagttctt cctgccagtt ttccagatta aggccatgca cactagtgag cagagccttc
     5341 gagttggcaa cgccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgctga
     5401 cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg
     5461 ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca
     5521 tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg
     5581 ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc
     5641 ataccatcac ctaccaccga gataatggcc agccgttcca tcaccgccag cggctccagt
     5701 aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc ccgcacacag
     5761 tcgctttgcg gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc
     5821 acggaaatac gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct
     5881 ttcatccccg ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg
     5941 accggtaatt cgccttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt
     6001 ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg
     6061 tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc
     6121 aacaacctcg catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc
     6181 caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa
     6241 ccgttgcgtc caagtaccac cagttcgcct ttttcattac cggcggtgaa gcctgccatc
     6301 agcaccatgt gatcagccgg aatacgactt gccgcaatac ggcgggtgga ctcagcaata
     6361 tcgacggtag attcgaggta atgccccact gccagtagtt tttcgaccgg atcgataacg
     6421 gtaacgttgt gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg
     6481 ccacggcaaa tcagcgcagc gttaatgcta tccgggcact gccctaacaa actaatgcca
     6541 tgcaggacat gttttatttg ggcaaattcc tggtcgacga aagttttcaa ttgcgccagc
     6601 gggaatcccg gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca
     6661 tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg
     6721 tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg
     6781 ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat ttgccactga tgtaccgccg
     6841 aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa aagcccgcac
     6901 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
     6961 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
     7021 gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt
     7081 caataaaatt ttaatttact catggcaggt aaccagttca gaagctgcta tcagacactc
     7141 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
     7201 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
     7261 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt
     7321 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct
     7381 tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
     7441 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
     7501 atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag cccggaatct
     7561 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
     7621 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactgcgcgc agtggtggcg
     7681 acagtgaaat cgacatcgtg taacgattca gccagtgttg ggaaaacttt aatattatca
     7741 ataacatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
     7801 tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc tgccccaata
     7861 ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc actcttcttg
     7921 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
     7981 ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc
     8041 ctgactgtac taacggttta attgttaaaa aatgctacat atccttctgt ttacttagga
     8101 taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg
     8161 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
     8221 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
     8281 ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt
     8341 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
     8401 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
     8461 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
     8521 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
     8581 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
     8641 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
     8701 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
     8761 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
     8821 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
     8881 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
     8941 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc
     9001 tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga
     9061 aaacccaggc aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct
     9121 gaaaccacac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
     9181 tacgccggat acgccggaaa tcatcgccac catccacggt gaaggttatc gcttctgtgg
     9241 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt
     9301 ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact
     9361 ggctttcatt ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat
     9421 gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat cgacagagtt
     9481 gagcagtccc cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt
     9541 gcgccagcct ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact
     9601 ggcgattatc catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata
     9661 aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt
     9721 gagcgtttca aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt
     9781 agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc
     9841 gaggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc
     9901 aaaccagctg ctttgccatt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg
     9961 caaaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt
    10021 acgcccggca ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag
    10081 cttcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc
    10141 ttgctctaac ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggcgcttt
    10201 caccacacca ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt
    10261 gatatttggc gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa
    10321 atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt
    10381 gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac
    10441 ggttttatcc tcttcctgta ccgtataaag ctcggtcaca ggaatagcga taagcggccc
    10501 aacaagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta
    10561 atcggcacgt tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac
    10621 tgcaccaaac agggtggtca ttttccagaa cagggaggat ttcaacatag tcttctcctt
    10681 tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg
    10741 gtgaagtcga agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc
    10801 gttaaacaaa cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc
    10861 atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat
    10921 accactaccg gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag
    10981 cgttatacga ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc
    11041 ctgatccagt aacgccggtt cagcagcaac attaacctcg gtgggcgtaa catgtagagt
    11101 gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc
    11161 cacatcaaca gcagtcaaaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa
    11221 cgtttccacc agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcag taaaacgagc
    11281 cactacttca ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag
    11341 tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc
    11401 cagcttcacg cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag
    11461 atcgggaaga ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc
    11521 aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcga tccccaacaa
    11581 aatggcgctg gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc
    11641 gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc
    11701 aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctac gcgcaccata
    11761 ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt
    11821 atttgccgaa tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt
    11881 gcgcactttg ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa
    11941 cgcctgagcc agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc
    12001 cgccagcaac gttgcggtat cgattaacgt cccctccgtt gctcttcgca cgcccggttt
    12061 aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata
    12121 gcccagcagc aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg
    12181 cgatgagtat taatcggaga aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt
    12241 ttaatgtggg tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc
    12301 attagttgct ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa
    12361 aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca
    12421 ttcaattcaa aatgaccaat acggatgacg ggagacggcg acgagaactt cttcacccga
    12481 cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag
    12541 tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg
    12601 aacagtacag gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccgctg
    12661 atatcaggca aaccaacatc gagaatcatg acgtcgggaa cctgctggcg agctttatcc
    12721 agcaccggca agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag
    12781 accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata
    12841 aatcgcctct gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc
    12901 gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca
    12961 taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc
    13021 tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga
    13081 caagaaatag ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct
    13141 gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc
    13201 acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct
    13261 tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat
    13321 ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca
    13381 tattgattaa gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta
    13441 ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg
    13501 tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg
    13561 accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg
    13621 cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg
    13681 ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga
    13741 ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta
    13801 cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat
    13861 ggagcgcctt tggtattcgc ccgccgctac gtctgggtga attcacaatg ccagagcaca
    13921 aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc
    13981 tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg
    14041 gcaacgcgcc gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg
    14101 ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg
    14161 gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct
    14221 atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc
    14281 caatgctcaa cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag
    14341 acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc
    14401 gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt
    14461 caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac
    14521 agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca
    14581 gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc
    14641 ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag
    14701 gaatacgccc gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg
    14761 tcagagaatc gatatgtctt ttctccagca cacccatgtt taattcacgc aggcgagaat
    14821 caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc
    14881 ctaaatcgct actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca
    14941 tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct
    15001 cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc
    15061 gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttatctgtgc
    15121 gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga
    15181 aggatcctgc catattgcat ccgttgccgt cgagagcggt gtaccggaac agccctttgg
    15241 cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc
    15301 agaggctgat ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg
    15361 ggtggtgatt gaaaacacca gccagcgcgg cgaggcgcgt tcggctactt tgccgttacc
    15421 tgcggtgatt ctgcagaaag tgcgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta
    15481 taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa
    15541 actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa
    15601 tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg gcattcggtg
    15661 cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca
    15721 gcaacacctc ttccagccac tggcgcagct ccacgggcgc ggctttcagg ctgttagatc
    15781 cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt
    15841 cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg
    15901 gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca
    15961 ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg
    16021 attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgcc tcccccggtt
    16081 gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca
    16141 ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa taacggtagt aagcgtcata
    16201 agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc
    16261 cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc
    16321 gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag
    16381 gtaactggtg ccaatgttga tattcgtttc cggatccagc aattgcccag ggctgctata
    16441 accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag
    16501 gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat
    16561 cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt
    16621 gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg
    16681 aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt
    16741 ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtgttat cgagattcca
    16801 gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggcgc tgtcaacatt
    16861 ctgcggcgct ttatcaatct tcagctcata ctcttctccg atgcgttgtg ctgcaaccat
    16921 cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg
    16981 tccgcgttcc agcaataaat ccacctgcca gtaacgccat tcatctttct cttttgcttc
    17041 catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagtgccat
    17101 tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatgacgt catcgcgcca
    17161 tttcgcctgc tcgtcggtga catcgttgcc catcaaacgc caggcgacga tatcgcgcag
    17221 ctcctgaatc tgatcttcat taagctgctg cacctgggca agcgacggga tcatcagccg
    17281 tgcattttca gcatcctgcc gcgccacact ggcaaacgcc accgctgcca tttgacgggt
    17341 aaaatcggtc gctccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag
    17401 tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt
    17461 taccaggcca gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg
    17521 atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct
    17581 cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt
    17641 ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa
    17701 ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag
    17761 cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg
    17821 ttgattcatc agatcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc
    17881 cttcagtcca ggcatcattt gttcgaccac atccatttgt cgattatccc aggcctgctt
    17941 gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac
    18001 cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac
    18061 aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa tagatttatg
    18121 ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg
    18181 tgatcgacca actgggatta ggcggggaaa aaggctacac ttcgcctttg aaaactcatc
    18241 atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt
    18301 gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc
    18361 cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtctac cctgctgcgc
    18421 attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag
    18481 attggttacc tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt
    18541 gaagaagcgg tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg
    18601 tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag
    18661 atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg
    18721 ctacgtctgc cggactggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc
    18781 gtagcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc
    18841 aaccacctgg atgccgaatc cgtggcctgg ctggaacgct tcctgcacga cttcgagggc
    18901 accgtggtgg cgattaccca cgaccgttac ttcctcgata acgttgcagg ctggatcctc
    18961 gaacttgacc gcggtgaagg tattccgtgg gaaggcaact actcctcctg gctggagcag
    19021 aaagatcagc gcctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag
    19081 aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg taaagcacgt
    19141 ctggcacgct ttgaagagct gaacagcacc gaatatcaga aacgtaacga aaccaacgaa
    19201 ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt
    19261 aaatcctacg gtgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggca
    19321 atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctct
    19381 ggtcaggaac agccggacag cggcaccatc actttaggtg aaacggtgaa actggcgtcg
    19441 gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc
    19501 gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc
    19561 tttaacttta aaggggttga tcagggtaaa cgcgttggtg aactttccgg tggtgagcgc
    19621 ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa
    19681 ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc
    19741 ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac
    19801 atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac
    19861 gaagagtaca agaaacgcac gctgggcgca gacgcactgg agccgaagcg tatcaagtac
    19921 aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gtgaacgcct tatccggcct
    19981 acaaaccaag aaatttcaat acgttgtgaa tctccaggcc tgataagacg cggtaagcgt
    20041 cgcatcaggc attgagcacc gaatgccgga tacggcgtga acgccttttc cggcctacaa
    20101 cagccttagt atccgcaatt tcgtagcggg tcacccgtgc aggttttttc aaaccttgtt
    20161 taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaactta ttttatacct
    20221 cgcctttgag ccgtttcatc gcggttatct ctgctccccc atcatctccc gcaccagctc
    20281 cacgcagcgc aggaaacggc tgtcataatc gtcctcttca acccgcacga attcgatatt
    20341 gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc
    20401 gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc tccagcagga tcaccagatc
    20461 gaaacggtat tcatcaatca acgcctgtac gaacggatgc tcacgccctt cgtacttttt
    20521 gcagaacgcc tgggtagtga caaaatcagt gtcaataaac gccactttat tggcatattt
    20581 cactgcaaaa tcaatgtatt gtgcgtggcc cagcgcgatt ttatcgtaat cggaatactg
    20641 caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc
    20701 actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc
    20761 gccaccaagg atcgccacgg tacgtacaaa gaacggcttc acttccgtag gaatatattc
    20821 ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt
    20881 cggatcgacc agcaccgtat cgatccccag atgttccata tactgtggcg catcggcttc
    20941 ttccgaggtg tagatcaggt ccggctgaat ccccttttca gccataaact ttttgatgcc
    21001 gttgctccac acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg
    21061 aatgcgaata tttttctgat atttaaaagt ttgtaataac cagcgcagac gatccggcac
    21121 cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg tatcgtcaaa
    21181 acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg
    21241 tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg
    21301 aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat
    21361 tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg
    21421 ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc
    21481 tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta
    21541 acgccaaaaa tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc
    21601 gcccggcgaa aaccgtgttt cgccgcttct gaaattcgtt cctgaccgct gggcaccggg
    21661 cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgtgg cagcggtctg
    21721 tcacgcaggc tggaaaccat cgccagcagt aacgctaaat cggcactggt ttcggttacc
    21781 ttcacgccgc cgaccacgtt cacaaataca tcctgatcgg ccatttgcag accaccgtga
    21841 cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac tgccacgcgg
    21901 cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc
    21961 gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag
    22021 aaaattgccg aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc
    22081 agctcattca ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg
    22141 gcatcaccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc
    22201 agcgagccat ctttggttac gtgcccaacc atgacaatcg ccacaccgcg cgttttggcg
    22261 aagcgcgtca gataagccgc cgtttcacgc acctgcgcca cgctaccagg cgatgattgt
    22321 acatccgcca tatgcatcac ctggatcgag tcaattacca tcagtttcgg ttgctcttct
    22381 tcggcaatca ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc
    22441 ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca
    22501 tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc
    22561 cccgcacctg ggttaccgcc aatcagaatg gcgcttcccg gcaccacgcc gccgcctagt
    22621 acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg
    22681 gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta
    22741 cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag
    22801 gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg
    22861 cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg
    22921 gcgagcaatt acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca
    22981 gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc
    23041 cccagccctg ctgctttgat catcggcagg tcattagctc catcgccaat cgccacggtc
    23101 tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca aagttttcgc tttgtactgc
    23161 gcgtctacga tgtcgccaat cacattgccg gtaaatttac cgtccatgat ctccagttca
    23221 ttggctacca cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa gaaagtaaag
    23281 ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt
    23341 aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct
    23401 ttcagcgtcg ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc
    23461 cgttcggtta cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata
    23521 cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg
    23581 tgcgggattt tccccagcgg ggcgacatcc agttgcgctt catgggccag gcgcgtagcc
    23641 cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca
    23701 acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg tttatccagc
    23761 ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca
    23821 tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac
    23881 caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat
    23941 gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga
    24001 agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc
    24061 ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac caccagcgac
    24121 agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg
    24181 ttgcaccgct gatgcgtacc gactcaccag atgaaaaacg cattcaggcg atcctcgatc
    24241 agttaacgga tgaaagccgc atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta
    24301 tcgcccgttc aggcgaaagc gtcgaagtgc gcgaccggct ggcgctcgac ggtaaaaaag
    24361 caggcggcta ttttaaccag cagattgtcg agccaattgc gggtaaaaac ggaccgctcg
    24421 gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa caggtggata
    24481 acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga
    24541 cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg
    24601 ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa
    24661 gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt
    24721 ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt
    24781 ttctctggcg caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt
    24841 gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg
    24901 gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc aagccggagt
    24961 acgataaaac catctctacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg
    25021 ccgaagcgtc cggacgtaac gatctggtgg tgaaaacagt cgaaggcgac cgcaaagtct
    25081 caggctcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca
    25141 atgccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga
    25201 aaggcattac ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg ccggggatca
    25261 cccatgagca ggtttgcgag gccataacca aggccttttt cgcccattat ggcgagcgtg
    25321 tagaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct
    25381 ttgcccgtca gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc
    25441 tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata
    25501 tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa gccctcgccg
    25561 ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc gaagcgctgt
    25621 tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg
    25681 cggtaaggta actcgctgat taaaatacca aaggaaggga atatgatcga tctggaaaac
    25741 caggagcggg agattatcaa tttaatgtta agccagcgta tttcctggct agccgccgtg
    25801 cgtatcaggc ataaattatc acttgctgaa gtaagtaaaa tgttaggtat ctcgattaat
    25861 tctctaaaat agatcgaaaa gacggaacgg cttagctcaa acatcaaaag caaaatggga
    25921 gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat
    25981 aaatgaatag attcgtgaat agatacttga atgtatttta ttttttatcc atatgaaaaa
    26041 taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag ccggagcagt
    26101 cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac tctttatcgc
    26161 ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag
    26221 cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac agtgcagatg gtcagggctt
    26281 tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga
    26341 tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg
    26401 agaacaggtt accaacgcga gcatcaatgc ccagtgcttt agccgcgtct accgcgttac
    26461 gcaccatgtc aaagtcagcg atagcggcaa agtcatgatc tttaaaacgg atgcggttaa
    26521 ctttggaatc ggtgcaggca cccataccga taacgacgtc gcgcagtttt acgtgcggca
    26581 gaactgcgcc acaggaaccc acgcggataa ttttcttcac gccgaaatcg gtgatcagtt
    26641 ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc
    26701 ggcctttgta agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat
    26761 cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta
    26821 cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt ttatccttat
    26881 caacatgttt tcaccctctc cctgagggag agggccgggg tgagggcatc aagccgcacc
    26941 cattatcaga acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga
    27001 gtctggccga tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg
    27061 ccgtaaacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatca
    27121 caaccgtggt cagcagtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc
    27181 ggcagacggc ggtcgaacag ctccagaccc acagcataac cggcgacgtc gcgacggtgg
    27241 ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct
    27301 ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cagttgcttt cactttcttg
    27361 gtgataccgc agttggcgta gatgtctgca attttaccga cagaaaccac ctgaccgtgt
    27421 ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caggtcgtga
    27481 cggttaccgg tacgctggaa gttaccggct ttgtcaccga taaacggacg agcgataaca
    27541 cgaccgatgt tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag
    27601 agtttatcca ggccaaaggt ttcttcgtgg caggcaatct ggaacacaga gtcagcggag
    27661 gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc cagaatgacc
    27721 gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc aaccagttta
    27781 tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaataga
    27841 accgggacac cggctatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg
    27901 tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa
    27961 ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc
    28021 ggacctttac gaccgttatc agcttcgcct ttggcacaag cttctgcgat atgacccagg
    28081 gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag
    28141 tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa
    28201 atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc
    28261 ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg
    28321 ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaaa
    28381 cgcgccatat cagtaaagcc gacgctgtaa tcaatggtgt cagatgcctg acgacgaccg
    28441 ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct
    28501 tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc
    28561 tcaacgaagt cggttgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct
    28621 ttaccgttgt ccagcactgc ttgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt
    28681 ttgccggaga taagcatctc cacgcacagc gccatcgtga catcaaacag acgcgggtta
    28741 cgatactcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg
    28801 gaggccagta cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta
    28861 gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg
    28921 ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgcaag tttcttcgcc
    28981 agaatagagg cggtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg
    29041 tagaaacgtt tatccgccgg agccagtgag ctggtctgac cgataatcgc cacgccgacg
    29101 tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat
    29161 tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccagagat catcggaata
    29221 tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtcacatc gccgacgcca
    29281 ccagtggaat gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg
    29341 gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg
    29401 aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg
    29461 ccgttgataa agaaacgaat ttcttcatcg ctcagtgcat gaccatcacg ttttttacga
    29521 ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt
    29581 gccggatgcg tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag
    29641 cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcaccg
    29701 tggcccagcg ctttcaacag gcttgccagc aggctggaag caccaaagcg gtagtgacgc
    29761 gcatctgccc agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct
    29821 tccgcagtac gcacgccgcc cgctggtttg aaaccaacgg ttttttctac gcccatatca
    29881 cggatcactt ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc cactttaccg
    29941 gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc
    30001 agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc
    30061 gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt acccgccatc
    30121 agcgcgcggt acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg
    30181 gtttctgcca gcgcgatttc gatgtcatcg ttaccgtgtg ggaagttggt taccgtagcg
    30241 attcggattt ccggggtgcc ctgctctttc agcgttttgc gagcaatcgg gataaagcga
    30301 ggatagatac agatagcggc ggtattgccg accggggttt tggcctgatg acacagagca
    30361 attactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca
    30421 cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt
    30481 caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg atatacatca
    30541 caattaagga aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt
    30601 cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga
    30661 aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca
    30721 tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga
    30781 gcagaagcgc cattttctgg cactggaagc agaaaacaat ctgccttatc cgcagcttcc
    30841 tgccgaagcc cgccgcgcgc tggatgaggg tgtaatctgc gatatgtttg aaggtcatgc
    30901 gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga
    30961 atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct
    31021 ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt
    31081 gttgcaacca tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt
    31141 ctggcgttac ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc
    31201 tgattcgccc attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc
    31261 gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc
    31321 gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat
    31381 tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg
    31441 tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga
    31501 tgacttcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc
    31561 gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga
    31621 agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc
    31681 ggttaacttg ctgtgtaaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc
    31741 aaatgaagtg ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt
    31801 gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggagca gttccgatat
    31861 cggcaccacg ccgggcgcgc gtttgccgta tggtgatgag ccagatccga tcacccatct
    31921 gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct
    31981 cgacgaaacc atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa
    32041 agccggaatg cgtgaattta ctgccaatgt cagcggtaac gatctggttc gcgttaccgg
    32101 ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac
    32161 cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt
    32221 gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg
    32281 acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact
    32341 gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc
    32401 cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtttgcgagc
    32461 agtgtgatac ctgcctgaag aggtgtccgc aacatgccac gccaatggcg caatccatga
    32521 gcgtggacga agtgcttagc catgtccgca aagcggtgct gtttattgaa gggataacgg
    32581 taagcggcgg cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca
    32641 aaaacgatcc gcaactgcgc cacctcacct gcctggtgga cagtaacggc atgttgagcg
    32701 aaaccggctg ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcgt
    32761 gggggagcga atgtcatcaa cacttaaccg gacgcgataa tcagcagatt aagcgcagca
    32821 tctgtttgct tgcagagcgc ggcaagctgg cggaactgcg tttgctggta attccagacc
    32881 aggtggatta tttgcatcac atcgatgagc tggcgacgtt tatcaagaga cttggcgatg
    32941 ttccggttcg cctgaatgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg
    33001 cgagcgccac gccggaagac gttgagccgt tggctgatgc gttaaaggtg cgcggggtga
    33061 gccggttgat atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca
    33121 cagacttgta ggcatgataa gacgcgacaa gcgtcgcatc aggcattatg cgccgactgc
    33181 cggatgcggc gtgaacgcct tatccggcct acggttcggc acagacttgt aggcatgata
    33241 agacgcgt
//
LOCUS       DAJTVE010000035        32533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000035 DAJTVE010000000
VERSION     DAJTVE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..95
                     /locus_tag="OO439_001565"
     CDS             <1..95
                     /locus_tag="OO439_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6315956.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(137..1213)
                     /gene="glpQ"
                     /locus_tag="OO439_001566"
     CDS             complement(137..1213)
                     /gene="glpQ"
                     /locus_tag="OO439_001566"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6315957.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1218..2576)
                     /gene="glpT"
                     /locus_tag="OO439_001567"
     CDS             complement(1218..2576)
                     /gene="glpT"
                     /locus_tag="OO439_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6315958.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2849..4477
                     /gene="glpA"
                     /locus_tag="OO439_001568"
     CDS             2849..4477
                     /gene="glpA"
                     /locus_tag="OO439_001568"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6315959.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4467..5726
                     /gene="glpB"
                     /locus_tag="OO439_001569"
     CDS             4467..5726
                     /gene="glpB"
                     /locus_tag="OO439_001569"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6315960.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5723..6913
                     /gene="glpC"
                     /locus_tag="OO439_001570"
     CDS             5723..6913
                     /gene="glpC"
                     /locus_tag="OO439_001570"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6315961.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7106..8008
                     /locus_tag="OO439_001571"
     CDS             7106..8008
                     /locus_tag="OO439_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6315962.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8049..8852)
                     /gene="yfaU"
                     /locus_tag="OO439_001572"
     CDS             complement(8049..8852)
                     /gene="yfaU"
                     /locus_tag="OO439_001572"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6315963.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8870..10159)
                     /locus_tag="OO439_001573"
     CDS             complement(8870..10159)
                     /locus_tag="OO439_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6315964.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10216..11420)
                     /gene="rhmD"
                     /locus_tag="OO439_001574"
                     /pseudo
     CDS             complement(10216..11420)
                     /gene="rhmD"
                     /locus_tag="OO439_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11435..12217)
                     /locus_tag="OO439_001575"
     CDS             complement(11435..12217)
                     /locus_tag="OO439_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6315965.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12437..13639)
                     /locus_tag="OO439_001576"
     CDS             complement(12437..13639)
                     /locus_tag="OO439_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6315966.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13739..14281)
                     /locus_tag="OO439_001577"
     CDS             complement(13739..14281)
                     /locus_tag="OO439_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315967.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14560..14985
                     /gene="nudI"
                     /locus_tag="OO439_001578"
     CDS             14560..14985
                     /gene="nudI"
                     /locus_tag="OO439_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6315968.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15024..15626)
                     /locus_tag="OO439_001579"
     CDS             complement(15024..15626)
                     /locus_tag="OO439_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6315969.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15934..17073
                     /gene="arnB"
                     /locus_tag="OO439_001580"
     CDS             15934..17073
                     /gene="arnB"
                     /locus_tag="OO439_001580"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6315970.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17077..18045
                     /gene="arnC"
                     /locus_tag="OO439_001581"
     CDS             17077..18045
                     /gene="arnC"
                     /locus_tag="OO439_001581"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6315971.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18045..20027
                     /gene="arnA"
                     /locus_tag="OO439_001582"
     CDS             18045..20027
                     /gene="arnA"
                     /locus_tag="OO439_001582"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6315972.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20024..20914
                     /locus_tag="OO439_001583"
     CDS             20024..20914
                     /locus_tag="OO439_001583"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6315973.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20914..22566
                     /gene="arnT"
                     /locus_tag="OO439_001584"
     CDS             20914..22566
                     /gene="arnT"
                     /locus_tag="OO439_001584"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6315974.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22563..22898
                     /gene="arnE"
                     /locus_tag="OO439_001585"
     CDS             22563..22898
                     /gene="arnE"
                     /locus_tag="OO439_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6315975.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22898..23284
                     /gene="arnF"
                     /locus_tag="OO439_001586"
     CDS             22898..23284
                     /gene="arnF"
                     /locus_tag="OO439_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6315976.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23278..23544)
                     /gene="pmrD"
                     /locus_tag="OO439_001587"
     CDS             complement(23278..23544)
                     /gene="pmrD"
                     /locus_tag="OO439_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6315977.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23588..25009)
                     /gene="menE"
                     /locus_tag="OO439_001588"
     CDS             complement(23588..25009)
                     /gene="menE"
                     /locus_tag="OO439_001588"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6315978.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25006..25968)
                     /gene="menC"
                     /locus_tag="OO439_001589"
     CDS             complement(25006..25968)
                     /gene="menC"
                     /locus_tag="OO439_001589"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6315979.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25968..26825)
                     /gene="menB"
                     /locus_tag="OO439_001590"
     CDS             complement(25968..26825)
                     /gene="menB"
                     /locus_tag="OO439_001590"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6315980.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26840..27598)
                     /gene="menH"
                     /locus_tag="OO439_001591"
     CDS             complement(26840..27598)
                     /gene="menH"
                     /locus_tag="OO439_001591"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6315981.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27595..29265)
                     /gene="menD"
                     /locus_tag="OO439_001592"
     CDS             complement(27595..29265)
                     /gene="menD"
                     /locus_tag="OO439_001592"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6315982.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29354..30649)
                     /gene="menF"
                     /locus_tag="OO439_001593"
     CDS             complement(29354..30649)
                     /gene="menF"
                     /locus_tag="OO439_001593"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6315983.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30728..31033)
                     /gene="elaB"
                     /locus_tag="OO439_001594"
     CDS             complement(30728..31033)
                     /gene="elaB"
                     /locus_tag="OO439_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6315984.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31088..31549)
                     /locus_tag="OO439_001595"
                     /pseudo
     CDS             complement(31088..31549)
                     /locus_tag="OO439_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31614..32531
                     /gene="rbn"
                     /locus_tag="OO439_001596"
     CDS             31614..32531
                     /gene="rbn"
                     /locus_tag="OO439_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6315985.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8804 c         8139 g         7694 t
ORIGIN      
        1 tttcgttgtt ggctaaagaa aagccggtag aaggcagtcc actgttttta ctacaaatgt
       61 gcatggaaca attagtggcg attggaaaaa tttgatatga gacaggatgg cgcatgaacg
      121 ccatcctgca gtagcattac tctttattaa ggaattttac tgccttatca gggaaatcag
      181 taaacagccc atttacaccc gctttgttat acagagcatc atataactga ttcacatcag
      241 tagtgtattc aggcagttta tccgaccgca cggtataagg atgcactacc agtttattct
      301 gctgagcatc ttgcaccatg ccagtgagtt tgatattacc cggctgcgat gtttcctcaa
      361 tcaacatatg gtaatccggc ccgataccgt cggcatattc cgcgacttgc ttcatggcac
      421 ctggcttaaa catccagtca tagctgtaat taacccagct tccgtccggc tgtttctgct
      481 gcgtttcatt ccagtcggta taggcaatca gctgtaccag attgagatcc atgcccattt
      541 taggttccag ctcattctta atacgcttca gctcatcagc atcaaaacat tgcaaataaa
      601 ctttatcgtc cttaccggtg taaccatatt tcttcagcac ttccagcgtt tttgccgcaa
      661 tatccttccc ttcctgatga tggaaccacg gcgctttgat ttctggatag ataccgatat
      721 ttttcccggt agagtgattt aacccctgaa caaattcaat ctcttcttca aaggtgtgcc
      781 cccggaagtc ggacttaccc attgggaaac gccccggata agtctgtact tttttaccgt
      841 tttcaatatc gaaaccttcg gtaaatttca gcgacttaat ttcatccagc gtgaaatcta
      901 tcgcgtagta acgaccgtct ttgcgcgccc gatccgggaa acgatcggca acatcagtaa
      961 cacgatcgag ataatggtca tgcagaacaa ccagatggtc gtctttggtc atcaccaaat
     1021 cctgttccag ataatccgct ccctgcgcat acgccatcgc ttttgctggc agcgtatgct
     1081 ccggcaaata tccactggca ccgcgatggg cgatgactat tttttcgttg ctgtccgccg
     1141 ccattgcact gcttcccatg actatagtgc tcatcatgat cgccatgcta aggtttttca
     1201 gcttcaattt catgccatta gcctccgttg cgtttttgca gtaattgttc atggcgacgt
     1261 ttttcgccaa tcatcacaac aatcaacaag ataaccgcca gaatgctgcc gccaatcatt
     1321 accataaagc cgccatccca gccgaagaag tccacggtgt agccaacaat cgcgctcgcc
     1381 gccaccgaac cgcccaggta accaaacagc ccggtaaagc ccgctgccgt acctgccgct
     1441 tttttcggtg ccagttccag cgcatgcaga ccgatcagca tcacaggacc gtagatcagg
     1501 aagccgataa caatcataca aatcatatcg acggttgggt tacctgccgg gttcatccag
     1561 taaacgatag tcgcgatggt caccagtgtc ataaagaaaa cgccggttgc cccacggttg
     1621 ccacggaaga ctttatccga catccagccg cacagcagag tgcccggaat acctgcatat
     1681 tcataaagga agtaggccca ggaggattta tccagcgcga aatgcttaac ctctttcaga
     1741 taagtcggtg accagtcgag gatgccgtaa cgcagcagat aaacgaacac gttggcgatg
     1801 gcgatatacc acagcagttt gttcggcagt acgtactgca tgaagatttg cttcgccgtc
     1861 agctcctgtt ccgctttttc gttatagtcg tccggataat catttttgta ctcttcgatc
     1921 ggcggcaagc cacaggattg cggggtatcg cgcatcatcg caaaggcgaa taatgccacc
     1981 agaatggcgc agaaagcagg catatagagc gccgcatgcc agtcattgaa ccaggccatc
     2041 cccagcagga acagcagcgg cggaatacca ccaccgacgt tgtgcgcaca gttccacact
     2101 gacacaatgc cgccacgttc tttctgcgac caccagtgca ccatagtacg accacacggc
     2161 ggccacccca tcccctggaa ccaaccgcag aggaacaaca gtacaaacat caccgcaatg
     2221 ctcgacgtcg cccatggcac aaagcccata aacaacatca ctgccgccgc cagaatcaaa
     2281 cctgcgggca ggaaaacgcg cggattcgag cgatccgata ccgaacccat gatgaatttc
     2341 gaaaatccat aagcaatcga gatccccgaa agggcaaaac ctaaatcacc gcgtgagaat
     2401 ccctgctcaa ccagataagg catagcaagc gcaaagttct tacgaaccaa atagtaagcc
     2461 gcatagccaa agaatatccc caggaaaatt tgccagcgca atcgacgata agtcggatcg
     2521 atctccgcgg caggtaagcg cgctttgtgt ggcgctggtt taaaaatact caacattgat
     2581 agcctccgtg gcccgtggtc ttatttatga ttaacagcct gattcagtga gagaacctgc
     2641 cgtttcttga gttgccgcga tgttaagaaa acattcataa attaaatgtg aattgccgca
     2701 cacattatta aataagattt acaaaatgtt caaaatgacg catgaaatca cgcttcactt
     2761 tcgaatcatg agcgaatatg cgcgaaatca aacaattcat gtttttacta tggctaaatg
     2821 gtaaaaaacg aacttcagag ggataacaat gaaaactcgc gactcgcaat caagtgacgt
     2881 gattatcatt ggcggcggcg caacgggagc cgggattgcc cgcgactgtg ccctgcgcgg
     2941 gctgcgcgtg attttggttg agcgccacga catcgcaacc ggtgccaccg ggcgtaacca
     3001 cggcctgcta cacagcggtg cgcgctatgc ggtaaccgat gcggaatcgg cccgcgaatg
     3061 cattagtgaa aaccagatcc tgaaacgcat tgcacgtcac tgcgttgaac caaccaacgg
     3121 cctgtttatt accctgccgg aagatgacct ctccttccag gccactttta ttcgcgcctg
     3181 cgaagaagca gggatcagcg cagaagctat agacccgcag caagcgcgca ttatcgaacc
     3241 tgccgttaac ccggcattga ttggcgcggt gaaagttccg gatggcaccg ttgatccatt
     3301 tcgtctgacc gcagcaaaca tgctggatgc caaagaacac ggtgccgtta tccttaccgc
     3361 tcatgaagtc acgggactga ttcgtgaagg cgcgacggtg tgcggtgttc gtgtacgtaa
     3421 ccatctcacc ggcgaaactc aggcccttca tgcgcctgtc gtggttaatg ccgccgggat
     3481 ctgggggcaa cacattgccg aatatgccga tctgcgcatt cgcatgttcc cggcgaaagg
     3541 atcgctgctg atcatggatc accgcattaa ccagcatgtg atcaaccgct gccgtaaacc
     3601 ttccgacgcc gatattctgg tgcctggcga taccatttcg ctgattggca ccacctcttt
     3661 acgtattgat tacaacgaga ttgacgataa tcgagtgacg gcagaagagg ttgatattct
     3721 gctgcgtgaa ggggaaaaac tggcccccgt gatggcgaaa acgcgcattt tgcgggccta
     3781 ttctggcgtg cgcccgctgg ttgccagcga tgacgacccg agcggacgta acgtcagccg
     3841 tggcatcgtg ctgctcgacc atgctgaacg cgatggtctg gacggattta tcaccatcac
     3901 cggtggcaaa ctgatgacct atcggctgat ggctgaatgg gctaccgacg ccgtgtgccg
     3961 caaactgggc aacacgcgcc cctgtacgac cgccgatctg gcacttcctg gttcacagga
     4021 acccgctgaa gttaccttgc gtaaagtcat ctccctgcct gccccgctgc gcggttctgc
     4081 ggtttatcgt catggcgatc gcacgcctgc ctggctgagc gaaggtcgtc tgcaccgtag
     4141 cctggtatgt gagtgcgaag cggtaacagc gggtgaagtg cagtacgcgg tagaaaattt
     4201 aaacgttaat agcctgctgg atttacgccg tcgtacccgt gtggggatgg gtacctgcca
     4261 gggcgaactc tgcgcctgcc gcgctgccgg actgctgcaa cgttttaacg tcacgacgtc
     4321 cgcgcaatct atcgagcaac tttccacctt ccttaacgaa cgctggaaag gcgtgcaacc
     4381 catcgcctgg ggagatgcac tgcgcgaaag cgaatttacc cgctgggttt atcagggact
     4441 gtgtggtctg gagaaggagc agaaagatgc gctttgatac tgtcattatg ggcggcggcc
     4501 tcgccggatt actctgtggc ctgcaactgc aaaaacacgg cctgcgctgt gccattgtca
     4561 cgcgtggtca aagcgcgctg catttctcat ccggttcgct ggatttgctg agccatctgc
     4621 ctgatggtca accggtgaca gacattcaca gtggactgga atctttgcgt cagcaggcac
     4681 cagcccatcc ttacagtctt ctcgaaccac aacgcgtgct cgatctcgct tgccaggcgc
     4741 aggcattgat cgctgaaagc ggtgcgcaat tgcagggcag cgtggaactg gcccatcagc
     4801 gcgttacccc gctcggcact ttgcgtgcaa cctggttaag ttcgccagaa gttcccgtct
     4861 ggccgctacc cgcgaagaag atatgtgtag tggggataag cgggctgatg gatttccagg
     4921 cgcatcttgc ggcggcttcg ttgcgcgaac tggatctttc ggttgaaacc gcagaaatag
     4981 agctgccgga actggatgtc ctgcgcaata acgccaccga atttcgcgcg gtgaatatcg
     5041 cccgtttcct tgataatgaa gaaaactggc cgctgttact tgatgcgctt attcctgtcg
     5101 ccaatacctg cgaaatgatc ctgatgcccg cctgcttcgg cctggccgat gacaaactgt
     5161 ggcgttggtt gaatgaaaaa ctaccttgtt cactgatgct tttgccaacg ctgccgcctt
     5221 ccgtgctggg cattcgtctg caaaaccagt tacagcgcca gtttgtgcgc cagggtggcg
     5281 tgtggatgcc gggcgatgaa gtgaaaaaag tgacctgtaa aaatggcgta gtgaatgaaa
     5341 tctggacccg caatcacgcc gatattccgc tacgtccacg tttcgcggtt ctcgccagcg
     5401 gcagtttctt tagtggcgga ctggtagcgg aacgtaacgg cattcgcgag ccgattctcg
     5461 gccttgatgt gctacaaacc gccacgcggg gtgaatggta taagggcgat ttttttgcgc
     5521 cgcaaccgtg gcagcagttc ggtgtaacca ctgatgagac gctacgcccg tcgcaggcag
     5581 ggcaaaccat tgagaacctg tttgccatcg gttcggtgct gggcggattt gatcccatcg
     5641 cccaaggatg cggcggcggt gtttgtgccg tcagtgcttt acatgccgct caacagattg
     5701 cccaacgcgc aggaggccaa caatgaacga caccagcttc gaaaactgca ttaagtgcac
     5761 cgtctgcacc accgcctgcc cggtgagccg ggtgaatccc ggttatccag ggccaaaaca
     5821 agccgggccg gatggcgagc gtctgcgttt gaaagatggc gcactgtatg acgaggcgct
     5881 gaaatattgc atcaactgca aacgttgtga agtcgcctgc ccgtccgatg tgaagattgg
     5941 cgatattatc cagcgcgcgc gggcaaaata tgacaccacg cgcccgtcgc tgcgtaattt
     6001 tgtgttgagt cataccgacc tgatgggtag cgtttccacg ccgttcgcac caatcgtcaa
     6061 caccgctacc tcgctgaaac cggtgcggca gctgcttgat gcggcgttaa aaatcgatca
     6121 tcgtcgcacg ctaccgaaat actccttcgg cacgttccgt cgctggtatc gcagcatagc
     6181 ggctcagcaa gcacaatata aagaccaggt cgctttcttt cacggctgct tcgttaacta
     6241 caaccatccg cagttaggta aagatttaat taaagtgctc aacgcaatgg gtatcggtgt
     6301 acaactgctc agcaaagaaa aatgctgcgg cgtaccgcta atcgccaacg gctttaccga
     6361 taaagcacgc aaacaggcaa ttacgaatgt agagtcgatc cgcgaagctg tgggagtaaa
     6421 aggcattccg gtgattgcca cctcctcaac ctgtacattt gccctgcgcg acgaataccc
     6481 ggaagtgctg aatgtcgaca acaaaggctt gcgcgatcat atcgaactgg caacccgctg
     6541 gctgtggcgc aagctggacg aaggcaaaac gttaccgctg aaaccgctgc cgctgaaagt
     6601 ggtttatcac actccgtgcc atatggaaaa aatgggctgg acgctctaca ccctggagct
     6661 gttgcgtaaa atcccggggc ttgagttaac ggtgctggat tcccagtgct gcggtattgc
     6721 gggtacttac ggtttcaaaa aagagaacta ccccacctca caagccatcg gcgcaccact
     6781 gttccgccag atagaagaaa gcggcgcaga tctggtggtc accgactgcg aaacctgtaa
     6841 atggcagatt gagatgtcca caagtcttcg ctgcgaacat ccgattacgc tactggccca
     6901 ggcgctggct taaactcctt tctgatgccc ggtaagcatg tggttaccgg gcatttttgc
     6961 gtacacgatt ccgtgcccaa tgtatgcgtt gcaacgcagt gaaaattcct ctgaaaacgt
     7021 ctcgcaaagg ctgaaactgg cagatgtcaa aggcctggga taaccgtaat gtcgcgtcat
     7081 cataaatatc aggtgacgga caaccatgac cgaatcaaca acctcctccc cgcatgatgc
     7141 ggtatttaaa acctttatgt tcacacccga aaccgcacgg gattttctcg aaatacattt
     7201 gccagaacca ctgcgcaagc tttgcaacct gcaaacctta cgcctggaac ccactagttt
     7261 tattgaaaaa agtttacgcg cttactactc ggatgttttg tggtccgtgg aaaccagcga
     7321 cggtgacggc tatatctact gcgtgattga acatcaaagc tctgcagaaa agaatatggc
     7381 ttttcggcta atgcgctatg ccactgccgc catgcagcgt cacctggata aaggctatga
     7441 cagagttccg ctggtggtgc cattgctgtt ttatcatggc gaaacatcgc cctacccgta
     7501 ctcacttaac tggctggatg agtttgacga tccgcaactt gcccggcagt tgtacaccga
     7561 agcttttccg ttggtggata tcaccatcgt acctgacgat gagatcatgc aacatcggcg
     7621 tatagctctg cttgaactga ttcaaaagca tattcgcgac cgcgatttaa tcggtatggt
     7681 cgacaggatc accacgcttt tggttaaagg cttcactaat gacagccagc tacaaacact
     7741 gtttaattat ctgctgcaat gcggcgatac ctcccgtttc acccgtttta ttgaggagat
     7801 ttccgaacgt tcaccactac aaaaggagag attgatgact attgctgagc ggctacggca
     7861 ggaaggacac caaattggct ggcagaaagg tatgcatgaa caagccatta aaattgcttt
     7921 gcgcatgctg gaacagggca ttgatcgtga ccaggtgctc gcggccaccc agctaagcga
     7981 agccgatctg gcagcgaata accactaatt aacacaggcc cacagccgat ccccatgggc
     8041 ctttgatatc aataactacc ttttatgcgt ggcccatttt tgcctgattt aaacatcgcc
     8101 agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc
     8161 gctccccacg ccaggcattg ctgcgccata tcaggagcca cagccagaaa accagccgct
     8221 ttacccgcag cacggatccg ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc
     8281 ccggcgttat ccgggtagcc caacgacgca gaaagatccg ctggtccaat aaacacgcca
     8341 tcaatccctt cgacgtcgag gatctcgtcc aggttatcca gtgccgtttt actttccacc
     8401 tgcactaaca gacaaagcga atcgttgact tgcgccatgt aattctcaat gcgtccccag
     8461 cgcgcagccc gtgccacact ggccccaaca ccacgctcac cgtagggagg atagcgcgtg
     8521 gcagacgcca cctgacgagc ctgatcggca gtatcgacca tcgggatcaa tagagtttgc
     8581 gcgccaatat ccagaacttg tttaatcagc gatttactgc cttccaccgg acggatcacg
     8641 ggttggctgg catagggcgc taccgcctgt agctggtgat aaagatcctg aatggtgttt
     8701 ggcgcgtgct ccccgtcaat cagcaaccag tcataaccgg aagtagcggc aatttctgcc
     8761 atataagaag tagtcgagct taaccacaga ccaatttgca cttcgccctt gcgtaaacgt
     8821 tctttaaagg gattggttaa taatgcgttc atcgtgttcc ttaacctatt taatgatgtg
     8881 ccacgtcggt ctgcgcaaca gtacgattta cacgcagagt gaaaataatc agcgaaccga
     8941 tgaccgccac cgctgccagt gtcagtaatc ctgccgcatc gctggcaaat aacgtttctg
     9001 ctttcacgcg caggatcggg gcaataaacc cgcccactgc accaaacagg tttacaaaac
     9061 caatccccgc agccagcgcc gtaccggaaa gcagttgtgt cggcatcgtc cagaacaccg
     9121 gctgcacggc aataaacccg atagctgcaa cacacagcgc tacgatcgcc attactggag
     9181 aaagcagccc ggacagacca ataccaatgc ccgccgccag taatgtcagc gctgcgacat
     9241 tacgccgttc gccggtttta tcggaatagc gcggaataag ccaggtccca aacaaggccg
     9301 caacccacgg aatggcggtg accaccgacg ctgtaaagcc cacttttgtt cccagcaatg
     9361 ccgcaacctg ggtcggcagg aagaaaatta atccgtaaac cgccacctga atggtcaggt
     9421 agataatcgc tagttgccag actcggccat tacgcagcgc atcgctcagc cgagaagtca
     9481 ctttctgttg ttcttcactt gccagttgat tgataagcaa cgttttttct tgtttactca
     9541 gaaaacgtgc ctgctccggt gtgtcatcaa gccaaaagaa tgtgaatacc ccagcaccga
     9601 ctgccaacaa tccttcaatc acaaacatcc agaaccagcc gggatgcccc ataaatccat
     9661 gcatctccaa cagcgcgccg gaaagcggtg atcccagtgt taacgccagc ggtgcgccca
     9721 tatagaacag ccccataatg ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat
     9781 aaatcatacc agggaaaaat ccggcctccg cagcaccaag cagagtgcga actatcagaa
     9841 atttcgcttc agtatcggcc catgccatgg ctgccgaaag aaatccccac agcagtgttg
     9901 tcgtaccaat ccaggttctg gcccccagtt tgcgcatcaa aagattcgcc ggaacaccca
     9961 gaaacgcata taccacaaag aaaatgcctg ctcccagcgc ataagcttca ttactcaacc
    10021 cggtatcaat ctggtaggtc tgtttggcaa aaccgatatt cgaacggtcg agaaacgcca
    10081 gcacatacag cgccaacata aacggaatta aacgcacacg gtttttcttc accacggcgt
    10141 caagcaaagc ggtgctcata aaaagctcct tagaatatgt gggcgttacc gttgtcacag
    10201 caacgcccgg agtgattagt ggctgtaggg gcgtttcaaa ttgcagtcac gattgagttc
    10261 gacgccaaaa ccgggtttat caagcactga tttatgaata cgaccattca ccggaaccgg
    10321 ctcattgagc agaatcgggt caaactgcgg acgcatcgtt gaacaatccg ggctggtcat
    10381 caggaattcg ctgaatggcg tattggtgaa ggtgatcacc gcatggtgag agtaaacaga
    10441 cgaaccgtgc ggcaccacca gttgccccct ggatttggcg attgcggcaa tttccaccag
    10501 cgtggttaaa ccaccgcacc agccaacatc cggctgcata atgtcgatac cggtttctga
    10561 aagcgtacga aaagattgca gtgtgccgtg gtgctcaccg ctggtgacca tcattccgac
    10621 tggcgcgttg cgtttcagtt cgcgataacc ttcatactgc tgtggcggca ggcactcttc
    10681 gatccatttc aggttatagg gcgcgcaagc gtgggccagt ttggtggcat agttcacgtc
    10741 ctgactcatc cagcagtcga gcattaacca gaaatcctca ccgcattttt cacgcatatc
    10801 cgcgaccata acggcatctt tgcggatccc cgcatcgcca tcatgtggcc cccagtgcgt
    10861 cggcattttg ccaccgataa agcccatctc ttttgccaga tccggacgcg cgcctgtggc
    10921 gtagaactga atctcatcac gaactgcgcc gcctaaaagt ttataaaccg gaagcccgac
    10981 cactttgccg aacagatccc acagagccag atcgacacag gaaatcgtgt tcatcaccag
    11041 gccaccagag ccggagtaat acagggtggc attgagcatt tgatcatgga tcagtttgat
    11101 atcactgaca catttaccct caatgaaacg gttaagatgt ttttcgacaa taaaacagcc
    11161 catttcaccg gctgtcgaaa cagcgaatcc ggtctggccg ttttctgctt cgacttcaac
    11221 aatcaacgtg ccaagaacgt taatgccaaa tgactggcgt gactgctcgt aatcgcggta
    11281 tttactcatc ggggtggcaa tatgatcgtc aatccagtga ttcgccccct ggtcgtgata
    11341 atcaccgcgc cagcaccttt ttctgctgtc gcaccgccag taaaccaggc gcgaacctgt
    11401 ttgatttttg gtagggtcat gatgttctcc attgttatga ggcctgtaag tcaaagggac
    11461 ttttccatcc caacagacgt gaaatatccc tggcgcaggc aatggccttg cccgccagat
    11521 aatcacggta ttcttcattg atttgtaagc gggtaccgac caccgagatc gcagcggtaa
    11581 gctcgttatt ggcgttaaac accggcgcag cgacacaacg gacatcgggg taatcttcgc
    11641 cgttgtcata gctccagccc tgacggcgaa tacgcgccag ttcttcgtga agttgctgtg
    11701 gatgagtaat cgttgtgggt gtcgcctgct cccagaccag cccttcgata atactttgct
    11761 gtaccgctgc aggttgccag gcaagcaggc atttacctat tccggagcga taaagcgaaa
    11821 ggctttttcc ttcatgggaa cgcacgctga tagtggctga tgactccact tttaaaatgt
    11881 aataagcgct gccgttatca ataatcccca ggtgacataa cagcccggtg gtatccatca
    11941 gttgcgtcaa acgcggtcga gccagttccc ggagatccat cttgcttaac gcatgaccag
    12001 aaagctccac cagcctggtc cacaaacaaa aattttcctg gtgatcaagg ctaagaaaac
    12061 gctgacgcct gagttcatta agcagcaaat aggccgtgct tttagggatt cccaatgtgt
    12121 caatgatcgt cgcagcacta caggggccaa tgcgggcaat caaattgaga atatcaatag
    12181 cccgtgtcag ggcgggaact ttgcttgatt ccaacatact ggactccagt cttagatact
    12241 ggaatcagtc tcgttgctca gggggagtaa aattgtgaag cgcatcaaat cccgctcctc
    12301 gtccagcgga ctggagaaat tacgcacaaa tttgtgaatc agtgcacgga ttttttctta
    12361 ttgagtacaa caggttggac aagttttgcc ggagggggaa tgtgccggtg cagtaaaccg
    12421 gcacagagtg gcggaatcag acagataagg tcatggactc aacaacctca atccagccat
    12481 gctcactggc gatatcctgg ccgtttaacc aacggcgcag catattcagt gccatcattg
    12541 cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc acacgcagag
    12601 cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa
    12661 cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag tgcgcagttt
    12721 gcgccagggt ttcttcctgt gaaggaacca cttcacacgc cagcaatgga gcacctgcgc
    12781 gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc aggctgaact
    12841 ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca
    12901 cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc
    12961 ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag cccattgtta
    13021 cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa
    13081 aggtagttaa acgcagacaa accggcgact gtggtaaaga aaaacgctcg cgcaggcgcg
    13141 gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga gtaaagaaca
    13201 tcaggcaacg attaagccgc acggcaaaac cacaggcggt gcctaccggg ttattgataa
    13261 attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt actcgtccac
    13321 gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc aggccttcac
    13381 cttttgctgt cgcagcggcg agtgcactta aatcatcact ggtcggtccc agcccgccgt
    13441 taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact aagtcatcaa
    13501 ggttatcccc caccgtattg cggcgagata atggcaaccc ctgatgaaag aaaaaatcgg
    13561 ccagccaggc agcgttagtg tcaacgattt gcccgtgtaa cacttcatcc ccggtggata
    13621 acatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact ataacgcaaa
    13681 gggagataag gcgtagtaag aaactgaatg gcgcgacaga atgccgcgcc ggaagggatc
    13741 agaaactggc gttaacccca acatacgggc cgtcagccac ggcgttgtcg cggttaccgt
    13801 ctttacccga cagattcagg tagcgataac ccgcctcaat actgactgga tgcataatgg
    13861 tgtaacgcgc gccagcattc gcttcctcat aacttttaat accgctggag agtgaatccg
    13921 gagagtagta atactcgcca aacaaacgga agctgttgcc aattttccac tgcaaaccac
    13981 ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg gttggtgtaa acgccttttc
    14041 cgccaacggt cgccattaac ggcccgagag gcagattcaa ccccagcccc acgccagcga
    14101 cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa cgccaggccc gtcgattcag
    14161 tcccaaaacc gacaccaata ttggtgtatt ctttacccgc ctgaccgctg atgctcattg
    14221 cattgaccga tgcagaaacc aacagcattc cggccagacc tgctagcgca atttttttca
    14281 ttatcccctt tcccacaagc aattaatcaa aattcccaaa gctgcgggat tgtactgcct
    14341 ggcacagagg attcaatgag cgaaaaaagg gatctagtca cttattgtaa ctaaatactt
    14401 cttaaataat taaacgccga aaaaataaac acactttacc gatacacaca aaaataataa
    14461 taagtgcagt tatcatcgca aaagagatta ctgtcacttt cacatatttc tgagttaccg
    14521 tattctcatg gtttcttttt atttataagg aatacccctg tgcgacaacg gactattgta
    14581 tgccctttga ttcaaaatga tggtgcttat ttgctgtgca aaatggccga cgatcgcggc
    14641 gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtctggcga acgaattgaa
    14701 gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt gacagaaatc
    14761 acgccgtgga ccttcagcga tgatattcgc accaagacgt atgcagatgg tcgcaaggaa
    14821 gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga agtgaaaata
    14881 aacgaagagt ttcaggacta cgcgtgggta aaacctgaag atctggtgca ttatgattta
    14941 aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg atgacagcca
    15001 tcactggctg tcagattgaa aatttagtgg ttaacgaatt ccccatccag ataaactttg
    15061 cctttttcca catgcatgac taaaccatcc agataatcag gtttaaatgt cgcatcacgc
    15121 ttatttttag caatatatgt caggcaatga ttatgggtga aaataacgat atttttatca
    15181 ggcgctttgc tttgtaagtc cttaattgca ctataaatct cattaccgca ctgaagaagt
    15241 cgtttatcta ccgtcagttt tttacccgcg gaaaaccagg tcgccgactg aatggtccgg
    15301 acggtattac tggaataaag atcgaaatca gggatgtcag cgctaaaagc gttgcccagt
    15361 tcacgggcat cctgggtacc tttaaccgta atacctgttt tatctgacaa gcattgattg
    15421 gttgaacgat cgcaacgttc agcatggcga aacaaaacga caaccgggtg ctgctgtgcc
    15481 agtctggcca atgttttatt gtcgatacgt ggcaaaccat tgctactcca ggcggcatga
    15541 gtacccagtc cggcaattgc agcgagcgcc agtaaaatga tgaaatattt ttttgacttc
    15601 aatgatgagc ggcaaaaagc taacacgtaa actccaccta tagacaagcg caaccagaca
    15661 attaccgtga aattgagcga catttctggc gataattagc agttggtgta atattaaaaa
    15721 tcctacgatg tcggcaaaat gcctcaaaat tttgccaaat gcaaagccta aataagaaaa
    15781 aatataaaaa tttcaatatt tacgtctaat attagtttct taaggttaag ttaatattct
    15841 atccttaaaa tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa
    15901 gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga
    15961 ccagcaatgg gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggatc
    16021 acaaccggtc cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag
    16081 catgccatcg cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa
    16141 attggcaagg gcgatgaagt gattacgcct tccctgacct gggtttcaac cctcaatatg
    16201 atttccttgt tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc
    16261 acgcctgaag cgatcgagtc agccattacg ccacgcacta aagccatcat tccggtgcat
    16321 tatgccggtg cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc
    16381 gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg acatattggc
    16441 gcaaaaggta ccgctatttt ttcatttcat gccattaaaa atattacctg tgcggaaggt
    16501 ggcctgattg taactgataa tgaaaacctt gcccgccagc tacggatgct gaaatttcac
    16561 ggtctgggtg tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc
    16621 ttaacaccgg gctataagta caatctgacc gatattaacg ccgcgattgc cctgacacag
    16681 ttagcaaaat tagagcacct caacacccat cggcgcgaaa ttgcccagca atatcagcaa
    16741 gcactggcag ctctcccctt tcagccatta agccttcccg cctggccgca cgttcacgcc
    16801 tggcatctgt ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg
    16861 atggaagcgt taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca
    16921 caaaaatatt atcgcgagcg ttttcccacg ttgtcgttac cgaataccga atggaatagc
    16981 gaacgcatct gttctttgcc gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc
    17041 atcacagccc ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag
    17101 aaagtctcgg tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg
    17161 cgcaccacca cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac
    17221 ggcagtagcg ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat
    17281 attgtgtcta ttttgcttaa ccgcaattac gggcaacatt cagcgattat ggcgggattc
    17341 agtcacgtta ctggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa
    17401 gaaatccccc gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc
    17461 cagaaccgcc aggacagctg gtttcgtaaa acagcttcga agatgattaa caggcttatt
    17521 cagcgcacca ccggcaaagc gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt
    17581 catattgtcg atgcgatgtt gcactgccat gaacgtagca cctttatccc gattctggcg
    17641 aatatcttcg cccgccgtgc cattgaaatt ccagtacatc atgccgagcg tgagtttggt
    17701 gaatccaaat acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt
    17761 accacaacgc cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt
    17821 agcattgcgg tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa
    17881 ggcgtcttta tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg
    17941 ggattgctcg gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat
    18001 tttgttcagc aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt
    18061 ttgcctacca cgatatggga tgcctcggta ttgaagctct gctggctgcc ggttacgaaa
    18121 ttagcgccat ttttacccat accgataatc ccgctgaaaa agccttttat ggttcggtgg
    18181 ctcgtctggc ggcggaaaga ggcattccag tttatgcgcc ggataacgtt aatcatccgc
    18241 tgtgggtgga acgcattgcc caactctcac cagaggtgat tttctctttt tattatcgcc
    18301 atcttatttg cgacgagatt ttgcagctcg ctcccgcagg tgcatttaat ctgcacggtt
    18361 cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa
    18421 cggaaacggg cgttacattg caccgaatgg taaaacgtgc cgatgctggg gccattgtgg
    18481 cgcaactgcg cgttgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt
    18541 gccatgccgc gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc
    18601 tggaaattgc ccagcgcgaa aacgaagcca cctgttttgg tcacagaacg ccagatgaca
    18661 gtttccttga atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg
    18721 atccgtggcc gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc
    18781 gtgttcatcc tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc
    18841 tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta
    18901 ctatgcaggg ctcgcaatta gcgcagatgc tgggcctggt gcaaggttca cgcttgaata
    18961 gccagcctgc ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct
    19021 ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc
    19081 tggatattgg cagcgatgcg ataagccgtt ttctgaatca tccgcatttt cactttgtcg
    19141 aaggcgatat cagtattcat tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg
    19201 tcttgccgct ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat
    19261 ttgaactcga ttttgaagag aatctgcgca ttatccgcta ctgcgttaag taccgtaagc
    19321 gaatcatctt cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg
    19381 aggaccattc taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtgt
    19441 caaaacaatt acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca
    19501 ccctcttccg cccgtttaac tggatgggac cacgactgga taaccttaat gcagcgcgaa
    19561 ttggcagctc ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccaatta
    19621 agctaattga tggcggaaaa caaaaacgct gctttactga tatccgggac ggtatcgagg
    19681 cgttgtaccg cattatcgaa aatgcgggaa atcgctgcga cggcgaaatt atcaacattg
    19741 gcaatcctga gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg
    19801 aaaaacatcc gctgcgccat cacttcccac cgtttgcggg ctttcgtgtt gtcgaaagta
    19861 gcagctacta cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg
    19921 cccgccgctg cctggactgg gaaccgaaaa ttgatatgca ggaaaccatc gacgaaacgc
    19981 tggatttctt cctgcgcacc gttgatctta cggataaacc atcatgacca aagtcggctt
    20041 acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc gtgccacgtc tgctggaaat
    20101 cttgagtaag cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg
    20161 ccgccatctc tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc
    20221 ggcatcgctt tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat
    20281 tggtcatgcc aatgccgata tcattcgtga agcggctaaa catcacgaag tcggcctgca
    20341 cgcctgggat caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat
    20401 cgacgatatt gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg
    20461 ttctgccgct gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca
    20521 tttgcgctac aacagcgatt gtcgtggggc catgccgttc cgtccactgc tcgaatcagg
    20581 aaaccctggc actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg
    20641 ggatgtgaaa gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg
    20701 cacgccggtt tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt
    20761 ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt
    20821 atcagagacg ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg
    20881 gctgggttgc caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc
    20941 ctcttcgcgt ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa
    21001 cccgatgaaa cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt
    21061 gtgccccatc tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac
    21121 agcattgggc aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt
    21181 gcgaccctgt taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa
    21241 cgtctggctc tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc
    21301 acttatgccg tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc
    21361 tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga
    21421 atcacctgcg gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta
    21481 agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg
    21541 ctggcggtta tcagttgcgt actgacggtt ctcccctggg gactggcgat agcgcagcgg
    21601 gagcctgact tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactggat
    21661 gatgcccaac atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg
    21721 ccgtggctgg gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc
    21781 gcaaccgtct atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa
    21841 ggtaaattgc ccacctatat tctttcctgc tttgcatctc tggcaatgct gatggcgcat
    21901 tacgctttgc tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac
    21961 atcgcttttg gcttcactgg cattattgcc acatttgtgg tctccccgtg gggaccaatg
    22021 aacacgccgg tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt
    22081 tcgctatggg catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggtctttt
    22141 gccgcgcttt gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtc
    22201 atggaaggaa aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc
    22261 cgctatattc ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc
    22321 gatgacatca tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat
    22381 gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag
    22441 gaggggatta ttacactcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc
    22501 attccgcccg cagatgccat cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc
    22561 aaatgatctg gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc
    22621 agaaacaggc aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc
    22681 tgggactggc gctggcttgt cttggtcttg ccatggtgct ctggctgctg gtcttgcaga
    22741 acgtaccggt aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg
    22801 ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca
    22861 ttattggcgg cattgtgatc ctcgggagta cggtgtaatg ggcctgatgt ggggattatt
    22921 cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc
    22981 gccgatgacg cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg
    23041 gatactgtta ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct
    23101 acataaactt gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg
    23161 gattgcttca atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg
    23221 agtagcctgt attatgagcg ggttgatgct gattttcctg cccacgacaa aacaacgtta
    23281 ctgagttttc cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc
    23341 cgagaggact ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa
    23401 aggcgagagt aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt
    23461 tatcgcgccg ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca
    23521 cgattttttg accagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat
    23581 ttacagatta acagataaac ctattatcaa cggcaatgga atggcaaaat tgcgaagtga
    23641 aactaatgat ttcgtaatcc tggcgttgca cccactcttt taacgcctga cgtgaaattt
    23701 taataccgcc gtttttcagc tccggcggta aagttagcca gcgcaccggt tgttgaaaac
    23761 gagccagctt atctttcacc cattcgccaa gatcaacggt ttgctggtca tactccacca
    23821 ccgctaccgg tcgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa
    23881 ccgcaggatg tgcagcaatt acgcgctcga cttcttccgg ctgaataccc tctccgccac
    23941 tgaagaacag attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc
    24001 gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc
    24061 agtaaccttc tgccatactg gcagcccgca gccacacttc attattaacg attttcactt
    24121 cccgacccgg cagcggcgaa ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg
    24181 tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct
    24241 gttccgtcaa ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg
    24301 aactgcggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc
    24361 ctgccagcat ttgctccaat ggctgtttat cacgcaccgt catccgcgca ccagcgtata
    24421 accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt
    24481 catcgtgatc gccaaacgga atcagcgata acacaccttg cgcactggca agatgggcct
    24541 gataagtatg gacagcggct ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc
    24601 acagacgcgt cggctgccac gtagcggcat gtgcgccttc aacccgctga atgtgcagcg
    24661 acgttaatgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat
    24721 tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg
    24781 ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca
    24841 acatcacgcc gctgccctca accacaccct gcgccgcaaa gcccgtagct aactcatcga
    24901 cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag gcgatggctt
    24961 ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata acaactgctc
    25021 cagtacatca gcgtccacta acggcagtgg gctccccggc cagcgacgaa cctgctgcgc
    25081 ctgcatcaga tccagcgtgt ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat
    25141 ccgcgccagt tgcgttagac ctaagctcga ctcaatggaa gaactgatca ccgccgtcag
    25201 ccccagcgca tgcgccgcct gtacctgctc gcgtactttt tccagactgc ccgtgagcgt
    25261 gggtttgata actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg
    25321 caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc
    25381 gcgggttttg cacggctctt cgagaaacgc gatgcggtgg cgataatccg ggttaacata
    25441 tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg
    25501 cagatccgga attgcctcca gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta
    25561 caatcccact ttgaccttcg ccactttctc gcctggcata tcagcaagtt tgaggatcag
    25621 atcgtcagga tcgccattac acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt
    25681 atctgccagc tccgccaatg cacagcttac gccaaaggca acggaaggca tctgcggtag
    25741 ctcgccatcg cctgccagcc agttatttac ccaagcaagc agcacaattt gcgcctcttc
    25801 ccaggtttcc tgactgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc
    25861 ttcacgcagg caaacataca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccat
    25921 ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt
    25981 ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct
    26041 tcttccgtca tgtagaacag catggtggcg ttgcccgcca gctcctgcag acccgcctga
    26101 ccgtcacagt cggcgttcag cgcagctttc aggcagcgca gcgccatcgg gctgttttgc
    26161 agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg
    26221 ttgacaaggc ccatatccag cgcctgtttt gcatcgtact gacggcacag gaaccagatt
    26281 tcacgcgctt ttttctgtcc gacgatgcga gccatgtagg aagcgcccca gccgccgtcg
    26341 aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgccgc gatagtcagg
    26401 tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt agccagccac catcgcgaca
    26461 accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag gtgatgtacg
    26521 ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag
    26581 aacgctttat cgcctgcgcc agtcagaatg atcacgccga cgttgtcgtc ataacgcgca
    26641 tctgccagtg cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc
    26701 ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctca
    26761 aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa
    26821 atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg ataacgccag
    26881 cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca
    26941 gttccgccgc caaggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa
    27001 atgtgcgggc gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca
    27061 gcatggcggc aagggttgcg ccattattgt tactgcgcag cgccaccagc tcccggcgtt
    27121 gatcgtcatt gagcgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta
    27181 acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgcgcgtt
    27241 gttcagcatt ttgcaaccca ggatgcccgc cttcgacaac aaccccacaa agccccggca
    27301 tcccctggca agccgccatc atcgccaccc gtcccccaag cgagtacccc accaaccaga
    27361 agttaaggat gttgtaacta accagggttt tacgcagtaa gtcggtgaca tcatcaaatc
    27421 catcgacgct aatcgccgcc gaaccaccgt gacctgggag ataaacatac aaccgtgagt
    27481 agtcggcaaa cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca
    27541 aaaacaccag ccagggtaaa cctggtttgc cgtgttttgc ctgcgcgtgc aggatcataa
    27601 atggcttacc tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac
    27661 catttcaatt accgtggtgg ttggcgtgcg ccaggcatcg gcaagtgccg tttcaagttc
    27721 ctgccagttt tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg
    27781 gacgttttgc ggcatcagat agaaacgctc gcgctcgctt ttcggcgttg gcagcagcga
    27841 gaaaatttgc ccgccgttgt tgttcaccac aattaatatc agcggcgcgg aaacctgacg
    27901 caataacgcc agcgcgttga gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt
    27961 cggtttgccg cttgcccgct gaacgccggc ggcagtcgag agcagcccgt cgataccgct
    28021 ggccccacgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag
    28081 acgtaccacc aggctgttac cgacaaacaa ttgcccctgt tcaggcagat agtcgctgat
    28141 gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc
    28201 ctgttccgcc aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag
    28261 ctccagccag tcggcaatat tggcaattaa gcggcgtcca cgatggtgcg ccggatcaag
    28321 ccgcccttcg atgtcatcca caatccagta ctcttcgggt tcacagcttg cctgccattg
    28381 caggagccgt ttgcccgtca ggctgcttcc cagttgcacc acaatttgcg cctgttgcag
    28441 ttcgctggtc gctttggcat tgcctaacca gagatcggca cacggcagcg gctgtccggt
    28501 ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt tgcgcccaca gtgcaacttt
    28561 tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgttttt gtcgccagaa
    28621 gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc
    28681 gtcctgccac cagtcgccca gccgctgttg ccagctaagc ccggtgtcgt ccatttcgcc
    28741 atacagcggt tcggcaaacg ggcagttgat atggactcca ccagaatgaa gcgtaccgag
    28801 agcatggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa
    28861 tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tgacgaatcg cctgattcgc
    28921 gccgcagtca atcagctccg gcgggcgatc ggcggttaac aggatcagtt tttctccggt
    28981 taacccagct tcaatcagtg ccggatagag atttgctacc gccgtaccgg aggtcacaat
    29041 caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg
    29101 ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg
    29161 tgtagaacgc gagccagggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc
    29221 cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat
    29281 caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg
    29341 atgcgactca ttactattcc atctgtaata aagtacgcag ccctgccgct ttgttgtcga
    29401 tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatataatc
    29461 gcacgacatt gccgctaatt tttgctgagc gcagggaaac gcagaattcg ctttgctgta
    29521 atgagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga
    29581 taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat
    29641 gtaaacagat cacattatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct
    29701 gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct
    29761 gtaaccgttg gcagatatct tccaccacca gcatattctc gcgctggttt ttatcatccg
    29821 ccatcagcca ctcacctaac tgctgcgcct gcttatcatc agggtgattt gctaccgttc
    29881 ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag
    29941 agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca
    30001 gtcgacgact ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag
    30061 ttgcccgagc gagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt
    30121 cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat
    30181 gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctctt gccttaatcg
    30241 catcgtgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac
    30301 agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg cgacgggtca aacgcattca
    30361 gcccccaaat gcgtaagtcg gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt
    30421 ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt
    30481 gttgccagta gaattgcggg tatgtttgct gactggccag ccagctcaag gcatcaaaag
    30541 cgtctttgag agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt
    30601 gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc
    30661 cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag
    30721 ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccaacggcc gcgcccacac
    30781 caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaact gcctgcttcg
    30841 cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt
    30901 tttctgcacg cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga
    30961 gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa
    31021 actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg
    31081 ttgttgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt
    31141 catagacctc cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct
    31201 gcgccccgag gtaaacaggc ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac
    31261 tcatcagttg ctgccccact ttttcgccac gcaacgcttc actgacaatc acccgaccaa
    31321 taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatatgcc accagttcat
    31381 catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct
    31441 gataagggca attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa
    31501 gctgggaaac cgataattca gagtgatgca gatcttacca ttcaatcata atgctctcct
    31561 tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat
    31621 taattttttt aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat
    31681 tgcttaacct gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca
    31741 cccagcatca gctactgcat accgccttta accctgggaa actggacaag atttttatca
    31801 gtcaccttca tggcgatcat ctctttggtt tacccggctt gctgtgcagt cgttctatgt
    31861 caggcattat ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa
    31921 ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg
    31981 ctggcgaaat tctcgatgat ggcctgcgca aagtgaccgc ttatccgatg gaacacccac
    32041 tggaatgtta tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg
    32101 cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa
    32161 caatcacgct ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc
    32221 caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg
    32281 ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag
    32341 ccaatagtcg cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag
    32401 tcggcaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc
    32461 tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt
    32521 ttaacgttta act
//
LOCUS       DAJTVE010000036        32026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000036 DAJTVE010000000
VERSION     DAJTVE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..252)
                     /locus_tag="OO439_001597"
     CDS             complement(<1..252)
                     /locus_tag="OO439_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6315986.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSA"
     gene            359..655
                     /locus_tag="OO439_001598"
     CDS             359..655
                     /locus_tag="OO439_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6315987.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(789..1214)
                     /gene="arsC"
                     /locus_tag="OO439_001599"
     CDS             complement(789..1214)
                     /gene="arsC"
                     /locus_tag="OO439_001599"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6315988.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1227..2516)
                     /gene="arsB"
                     /locus_tag="OO439_001600"
     CDS             complement(1227..2516)
                     /gene="arsB"
                     /locus_tag="OO439_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6315989.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2570..2923)
                     /gene="arsR"
                     /locus_tag="OO439_001601"
     CDS             complement(2570..2923)
                     /gene="arsR"
                     /locus_tag="OO439_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6315990.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3462..3635)
                     /locus_tag="OO439_001602"
     CDS             complement(3462..3635)
                     /locus_tag="OO439_001602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6315991.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3663..3746
                     /locus_tag="OO439_001603"
     CDS             3663..3746
                     /locus_tag="OO439_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6315992.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3800..5152)
                     /gene="gorA"
                     /locus_tag="OO439_001604"
     CDS             complement(3800..5152)
                     /gene="gorA"
                     /locus_tag="OO439_001604"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6315993.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5224..6066)
                     /gene="rlmJ"
                     /locus_tag="OO439_001605"
     CDS             complement(5224..6066)
                     /gene="rlmJ"
                     /locus_tag="OO439_001605"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6315994.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6269..8311
                     /gene="prlC"
                     /locus_tag="OO439_001606"
     CDS             6269..8311
                     /gene="prlC"
                     /locus_tag="OO439_001606"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6315995.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8319..9071
                     /gene="rsmJ"
                     /locus_tag="OO439_001607"
     CDS             8319..9071
                     /gene="rsmJ"
                     /locus_tag="OO439_001607"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6315996.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9120..10589)
                     /gene="dtpB"
                     /locus_tag="OO439_001608"
     CDS             complement(9120..10589)
                     /gene="dtpB"
                     /locus_tag="OO439_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6315997.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10907..11341)
                     /gene="uspA"
                     /locus_tag="OO439_001609"
     CDS             complement(10907..11341)
                     /gene="uspA"
                     /locus_tag="OO439_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6315998.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11732..12067
                     /gene="uspB"
                     /locus_tag="OO439_001610"
     CDS             11732..12067
                     /gene="uspB"
                     /locus_tag="OO439_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6315999.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12311..13810)
                     /gene="pitA"
                     /locus_tag="OO439_001611"
     CDS             complement(12311..13810)
                     /gene="pitA"
                     /locus_tag="OO439_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6316000.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14042..15244
                     /locus_tag="OO439_001612"
     CDS             14042..15244
                     /locus_tag="OO439_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6316001.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15560..16543)
                     /locus_tag="OO439_001613"
                     /pseudo
     CDS             complement(15560..16543)
                     /locus_tag="OO439_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16943..18550
                     /locus_tag="OO439_001614"
     CDS             16943..18550
                     /locus_tag="OO439_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6316002.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18812..20434
                     /locus_tag="OO439_001615"
     CDS             18812..20434
                     /locus_tag="OO439_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6316003.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20800..21867
                     /locus_tag="OO439_001616"
     CDS             20800..21867
                     /locus_tag="OO439_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6316004.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21864..24599
                     /gene="rbbA"
                     /locus_tag="OO439_001617"
     CDS             21864..24599
                     /gene="rbbA"
                     /locus_tag="OO439_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6316005.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24599..25723
                     /locus_tag="OO439_001618"
     CDS             24599..25723
                     /locus_tag="OO439_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6316006.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25796..26069
                     /locus_tag="OO439_001619"
                     /pseudo
     CDS             25796..26069
                     /locus_tag="OO439_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26066..26425
                     /locus_tag="OO439_001620"
     CDS             26066..26425
                     /locus_tag="OO439_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6316007.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26545..26946)
                     /gene="nikR"
                     /locus_tag="OO439_001621"
     CDS             complement(26545..26946)
                     /gene="nikR"
                     /locus_tag="OO439_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6316008.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26952..27758)
                     /gene="nikE"
                     /locus_tag="OO439_001622"
     CDS             complement(26952..27758)
                     /gene="nikE"
                     /locus_tag="OO439_001622"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6316009.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27755..28519)
                     /gene="nikD"
                     /locus_tag="OO439_001623"
     CDS             complement(27755..28519)
                     /gene="nikD"
                     /locus_tag="OO439_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6316010.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28519..29352)
                     /gene="nikC"
                     /locus_tag="OO439_001624"
     CDS             complement(28519..29352)
                     /gene="nikC"
                     /locus_tag="OO439_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6316011.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29349..30293)
                     /gene="nikB"
                     /locus_tag="OO439_001625"
     CDS             complement(29349..30293)
                     /gene="nikB"
                     /locus_tag="OO439_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6316012.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30293..31867)
                     /gene="nikA"
                     /locus_tag="OO439_001626"
     CDS             complement(30293..31867)
                     /gene="nikA"
                     /locus_tag="OO439_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6316013.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
BASE COUNT         8157 a         7929 c         8384 g         7556 t
ORIGIN      
        1 cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc
       61 tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat
      121 tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag
      181 caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca gcatcgcaag
      241 ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa tcgaaatgaa
      301 attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg ggtgtcatat
      361 gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc gtttgttcat
      421 atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt tgcaaaagca
      481 gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat cggtaggact
      541 ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg caatgctgga
      601 agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg cctgatgcgc
      661 tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg tcggataaga
      721 cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg gcagtggata
      781 tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc atcttccttg
      841 gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga agggcggcac
      901 aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg gtgctgaagc
      961 ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag ctcctcatac
     1021 ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg
     1081 accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg ttctgtgccg
     1141 ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt gtgataaatg
     1201 gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag cgccagcgca
     1261 gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa gtaatatccc
     1321 cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt agccaggcta
     1381 ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata aaccatcgct
     1441 tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc
     1501 ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc gagcgtcgcg
     1561 gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc cgttaatccg
     1621 gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac aatctgccag
     1681 ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc gacgacaaat
     1741 aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg
     1801 aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt agcaggatct
     1861 ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg aatatctttg
     1921 cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc caccggcacc
     1981 atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac gatattcacc
     2041 aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc cgccatcacg
     2101 aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat gacaatcggc
     2161 gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc accgagcagg
     2221 acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac gtgcagcgcc
     2281 gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac ggcgataaac
     2341 gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg atgaaccacg
     2401 cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc taaacctttc
     2461 ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag taacataatg
     2521 cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt taactgcaaa
     2581 tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc gcctgaacct
     2641 tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct ggaatatgcg
     2701 gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc ccgctttcac
     2761 gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca gtacaaagat
     2821 cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga cgggtttcat
     2881 cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc tcctgattgt
     2941 ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact taacgaatgt
     3001 gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt tgaaaggcgg
     3061 tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc agcctgagtt
     3121 ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat tagccagtgg
     3181 ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc ggtatatatc
     3241 gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg tccgaatccg
     3301 gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag ttggtggctc
     3361 tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac tgaaactatt
     3421 gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt tagctctgag
     3481 gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg
     3541 gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa tgagacatgc
     3601 gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac
     3661 gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg gaactgatcc
     3721 gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact
     3781 actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc gttgggtgaa
     3841 tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc accgcgaagc
     3901 cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttctg
     3961 gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca
     4021 tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct
     4081 gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt
     4141 tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac
     4201 ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa
     4261 taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt tcgttagttt
     4321 taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa
     4381 tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg
     4441 catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct tcggcgttca
     4501 tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc gcatgtttac
     4561 gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc tcaacggcga
     4621 tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat
     4681 cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga ccgcctgtgg
     4741 cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt ttggcatcaa
     4801 cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg
     4861 aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaact
     4921 tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct tcacggattt
     4981 gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag gtgccgccca
     5041 gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg
     5101 cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacagagt
     5161 gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg
     5221 caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg gaaccagttt
     5281 gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg gcgggttaat
     5341 cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta ccgccagttc
     5401 aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc gcttaatttg
     5461 ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac gtttgtaacc
     5521 ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat
     5581 aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct ggaaaccgtc
     5641 ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca acgggtaatc
     5701 gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat
     5761 caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt ttaccacatt
     5821 gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgataccttc
     5881 gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc cggcccctgc
     5941 gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct
     6001 ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact
     6061 gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag
     6121 cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg
     6181 aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt tgagcgcttc
     6241 cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc cctttgaatt
     6301 gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc aggcgctgaa
     6361 cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa
     6421 tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct ccccggtcag
     6481 ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc aaaccctgcc
     6541 gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtaccg
     6601 cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga aagcggttga
     6661 taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga aacagcagcg
     6721 ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca acaacgtcct
     6781 cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg cggggatgcc
     6841 agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct
     6901 gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggctct
     6961 gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg
     7021 taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca
     7081 actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc
     7141 gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa
     7201 agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg agttgcagcc
     7261 gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgacga
     7321 gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg aagtggtgaa
     7381 acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc atccggatgt
     7441 acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct acctcgatct
     7501 gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag gccagatgcg
     7561 taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc
     7621 ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt tccacgagtt
     7681 cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt ccggtatcag
     7741 cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga
     7801 gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga
     7861 gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca
     7921 gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg atcagggggc
     7981 aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc catctccgtc
     8041 ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg cggcaggtta
     8101 ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga
     8161 gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg
     8221 ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc
     8281 gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg
     8341 aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg ctggagcacg
     8401 atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg
     8461 atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac
     8521 gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt
     8581 atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt gtgctggctt
     8641 cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg ctgctcgacg
     8701 acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag gagcgtttgc
     8761 agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc ccgcaggtgg
     8821 tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc
     8881 gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg gagcctgcac
     8941 gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca ccgctagcga
     9001 atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc tatgcaggta
     9061 cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat
     9121 taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa cagcatcact
     9181 actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata gacgggtaac
     9241 gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac atagccgccc
     9301 agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc
     9361 tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa ttcacccagg
     9421 ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg tgcatccgca
     9481 aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa catgccgaga
     9541 gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt gtaaatgcct
     9601 gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg
     9661 tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgac
     9721 gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg
     9781 acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg
     9841 gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc
     9901 catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag tttgctgaag
     9961 ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg
    10021 taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg
    10081 tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac
    10141 atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata gcacttcgaa
    10201 agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg
    10261 atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc
    10321 acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc gacatagccg
    10381 ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc
    10441 tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag
    10501 tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc
    10561 agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc atcctgaaaa
    10621 gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat
    10681 tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa
    10741 tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat tgcgtacgca
    10801 gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc caaaaagcaa
    10861 aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat tcttcttcgt
    10921 cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga cgtgcggaag
    10981 acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc atatcgtatt
    11041 tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg gtttcagtga
    11101 ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc
    11161 gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca gagtagttta
    11221 catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag actgctttct
    11281 ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt ttataagcca
    11341 tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc ttgtgccgtt
    11401 acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa agattcagtg
    11461 ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct tgcttacccg
    11521 ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa
    11581 caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt
    11641 ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg
    11701 aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc
    11761 tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt
    11821 agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt
    11881 tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg
    11941 ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc gtcggcagtt
    12001 tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg
    12061 gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct
    12121 cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc ctacataatc
    12181 tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca cgccgcatcc
    12241 ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc gctcgttttg
    12301 gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa agcagtaccg
    12361 cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta cgctgtaagc
    12421 cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga gtagtggaaa
    12481 ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc tgggcagaca
    12541 tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac
    12601 ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca
    12661 ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac aggcgctgat
    12721 cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca
    12781 taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt tccacatcgg
    12841 tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga tggcagtgga
    12901 actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtag
    12961 cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac
    13021 gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggcgcga
    13081 cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc gcgccgtgcg
    13141 aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac ggcggctttt
    13201 tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg
    13261 tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa
    13321 taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca
    13381 ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca
    13441 gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg
    13501 ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca
    13561 gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac
    13621 ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc gcgcgggtat
    13681 agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca
    13741 gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca
    13801 aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact
    13861 ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc
    13921 actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt aatattgatg
    13981 ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag aggagcgcaa
    14041 cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc
    14101 gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg
    14161 caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccagg
    14221 cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt ttacccagtg
    14281 ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa cgttagggca
    14341 actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa
    14401 gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg atgaaacagg
    14461 cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca tggcgaccgg
    14521 tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt
    14581 tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc ataaaccgtt
    14641 gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa
    14701 cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg gaccggcggt
    14761 gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc tgctaccgga
    14821 tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc agagcctgaa
    14881 aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc aactcgggca
    14941 aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac tgattagcac
    15001 attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac
    15061 gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc
    15121 tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg gctataactt
    15181 ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag cgaagtcgtc
    15241 ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag ctaattttga
    15301 tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct ccaggcttaa
    15361 tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt tataaaatat
    15421 gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac tatgcattaa
    15481 tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt
    15541 tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt gaaacgattg
    15601 agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga ataaaatatc
    15661 ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa aacaggcagg
    15721 tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc cagaccagtg
    15781 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa ataagaataa
    15841 tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta
    15901 ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa
    15961 acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg
    16021 cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta
    16081 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc
    16141 aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg ctccccgctg
    16201 catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg
    16261 caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat
    16321 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    16381 tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    16441 atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca tgacccgcga
    16501 cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata tatgttcact
    16561 ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa tttcagcctg
    16621 ttaatataac gacgagatat cattaaagac aaatgcatag cataactata gcggggttta
    16681 ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata tttatcctaa
    16741 gattaattct tattctgttg ttttttttac aattatcttc cagggatgga ttgttttcca
    16801 ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata taagtattat
    16861 tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg ttactaacaa
    16921 gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc acttctcttc
    16981 aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg gcaaggttga
    17041 taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt cgcaaataga
    17101 taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga gggtaaatga
    17161 acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta tagatattca
    17221 agaatataat agtttcgccg ataatactac taacacgttt atctttacaa ttataccaga
    17281 caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt gtaaggatgg
    17341 ttattatttt attaatttct ccggagataa atcagacata atttataaat cagacataac
    17401 ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc ttagcggtaa
    17461 ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg ataagtttga
    17521 tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg gtgtgattaa
    17581 cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag gcgctgtact
    17641 ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga gtttattatg
    17701 tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa ataataaatt
    17761 gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc aacatgtttt
    17821 tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag gcgatagatt
    17881 tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc gaaatatgga
    17941 tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa atcatgaaat
    18001 caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg ccggtgatac
    18061 gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga aagttttaac
    18121 cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg gtgctttgca
    18181 ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa atatatttaa
    18241 taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa gcaatgctgt
    18301 agcatccaca cctgtatatg aaaattattt taataacact actgctttca gaccaaaacc
    18361 tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat atggccatta
    18421 tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc taaattcctg
    18481 gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta gttgttttca
    18541 acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca gtatttcata
    18601 ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc tttagggact
    18661 ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga tttgtgcaaa
    18721 atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg ttaactatgt
    18781 attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg caggcactaa
    18841 cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta ccaacttcac
    18901 taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa gtattgccag
    18961 gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac ggttccaaca
    19021 atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag aaaaatatag
    19081 tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc agtttattgt
    19141 gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta attggtgcag
    19201 cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg ttgcagttac
    19261 tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat ttgatattaa
    19321 attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca attgttcacc
    19381 tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct cagtcggtgc
    19441 agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag gagaaaattt
    19501 ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa gggagaactt
    19561 taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca gaagagacat
    19621 tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca cgccatgtac
    19681 gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata aatttatcat
    19741 cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag ctggaaatca
    19801 tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac cacttgatga
    19861 ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca aatacaaaat
    19921 aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca ttataggacc
    19981 gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat tagcggcaat
    20041 tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata gccgggctat
    20101 ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac ctaaacttga
    20161 agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac ataacggtaa
    20221 tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg cccgtgacag
    20281 taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg gtgcagggcc
    20341 tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg gacttatgcc
    20401 tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga atattaatgt
    20461 tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc ctgcaactga
    20521 acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt tttatagatc
    20581 acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga caacattact
    20641 acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg gtcgtatttg
    20701 tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg gcagttgtaa
    20761 cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag taagcgccat
    20821 ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg gtggctgttg
    20881 cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga agcgacggaa
    20941 gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa agaaggccag
    21001 tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca ggagcagcga
    21061 ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc gcaggctttg
    21121 ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca acgccagtcc
    21181 gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca acgaggggct
    21241 atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg agctgcgctg
    21301 gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc ccgtaccaat
    21361 atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg tattgccgca
    21421 gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta tcgggttgct
    21481 gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga tctcagcgac
    21541 gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact tggcggtgaa
    21601 gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat cagttttgtc
    21661 gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg gctgaaactg
    21721 atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct ggaatatgtc
    21781 aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc gtggcctgac
    21841 gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc ccgcctgtcg
    21901 cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac aatattactc
    21961 tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc gggaagtcga
    22021 gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg atggtgctgg
    22081 gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc gcctggatgc
    22141 cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac gtcgattttt
    22201 tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat gagctgctga
    22261 ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc ggcgggatga
    22321 agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg atccttgatg
    22381 agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg atcgacagta
    22441 ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg gaagaggccg
    22501 aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact ggcagcgccg
    22561 aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata aatctgttac
    22621 cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa cctgaaaacg
    22681 cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc gttgccgttg
    22741 atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt tcgaacggct
    22801 gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc gaaggtgagg
    22861 cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt cgggtgggct
    22921 atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac cttgagttac
    22981 atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct gaaatgagcg
    23041 agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg ctcggcattc
    23101 gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta atcctcgatg
    23161 agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg atggtcgatc
    23221 tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac gaagcggaac
    23281 gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt acaccgcagg
    23341 aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc tatttgcagg
    23401 aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac accacccacg
    23461 cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc gaagcgctgg
    23521 aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg atcctgatgc
    23581 tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg gtgctcgacc
    23641 gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc cgttacttta
    23701 tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt gcgggcgata
    23761 tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt ggtacgcctg
    23821 tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg gtaaaaggtt
    23881 acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa tcgacacccg
    23941 ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg gacgtaaaaa
    24001 gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc ccgtcaatgc
    24061 taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac ctttacgtga
    24121 cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc gcgctgggga
    24181 tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta ccgcataaag
    24241 gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc accggaatgg
    24301 ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga acggcgatta
    24361 tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct tcgctggaag
    24421 ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact atcgcccgcg
    24481 gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc ccgttgctga
    24541 tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag gagggatgat
    24601 gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc tgctcggtga
    24661 taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt cgtcagcgac
    24721 cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg atcaatcgca
    24781 gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac cggagatgat
    24841 caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg cgataaatat
    24901 tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc aggtgaacgt
    24961 cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga atattatcaa
    25021 cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt tggtatcgct
    25081 ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg gggtgatggc
    25141 gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc tgatccgcga
    25201 gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt ttgagatcat
    25261 gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat tatcgctggt
    25321 gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc tgtttatgct
    25381 gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg ggacgatagc
    25441 gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc tgcaaatgct
    25501 ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca ttatgctgac
    25561 catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg gtgccggatt
    25621 cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt ttttcaccat
    25681 tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg aaacttgact
    25741 tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac atgccatgag
    25801 aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata tttaaaactc
    25861 ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca ttaggcggag
    25921 aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc gttgcgtgtt
    25981 tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc gtgcgtgact
    26041 ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact ctatggagat
    26101 tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc gtggcaaatt
    26161 cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc tgcaaaaaga
    26221 aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta ttgagccgta
    26281 tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc tgagtgaacg
    26341 ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta ttgaaacact
    26401 gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga atatctgcct
    26461 ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag ttaatatctt
    26521 ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca aatgcccatg
    26581 ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc ccatatcacc
    26641 tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca gcgtggcgac
    26701 ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt cgcgtttttc
    26761 gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct gagtggcctc
    26821 ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt tataaccacg
    26881 acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga tggtgactcg
    26941 ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc
    27001 gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg
    27061 gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg
    27121 cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag ctttttcagc
    27181 aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc
    27241 agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctggccgccg
    27301 cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc cttcagcatt
    27361 tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg catcggttca
    27421 cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc ctgaaatacc
    27481 atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc cagcggttcg
    27541 ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa ccgcgcgagg
    27601 gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct tttcagggtc
    27661 agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc accgtgggca
    27721 tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc ataccgtaga
    27781 gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg ttaaacagcg
    27841 tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg tcatccgcca
    27901 gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct tgtttttgca
    27961 taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg
    28021 gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc atcatgcgct
    28081 gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg gcgttttcca
    28141 gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cctaacgcca
    28201 ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg ctgcgcgggt
    28261 tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc gctttgccat
    28321 cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc agcgtcgcgg
    28381 cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg cgcccgcgtt
    28441 gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg atattacgta
    28501 gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc
    28561 gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg
    28621 ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa tcatcacgcc
    28681 ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg cgacgtgcag
    28741 catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca ccgcgcctgc
    28801 cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg ccagcacaaa
    28861 ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc acgcccagtg
    28921 cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc caaccataaa
    28981 gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca tggtggcctg
    29041 atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta atgtcagcac
    29101 cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca tcagccgcga
    29161 gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg gcgaaagcag
    29221 gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc tggtgagcgc
    29281 aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag
    29341 gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg
    29401 agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac
    29461 tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc
    29521 caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat gtgcatcccc
    29581 acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg
    29641 tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac gtccagcata
    29701 ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc
    29761 aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag atacaccgaa
    29821 aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc aagaaacgcg
    29881 atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca gataccgagc
    29941 ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag cgtggcgggc
    30001 aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca
    30061 agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccg
    30121 agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga
    30181 taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg
    30241 gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt
    30301 tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatattac
    30361 ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga taaaccgcct
    30421 cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg
    30481 tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat ccttgctgtg
    30541 cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg
    30601 gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga
    30661 tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct
    30721 gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc
    30781 gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc caaccggctt
    30841 tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg ccgagattag
    30901 cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca tataacgcgt
    30961 tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact gccagctcgt
    31021 tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt
    31081 gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt aataaccctt
    31141 cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt
    31201 ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt
    31261 tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac ggtccggtgc
    31321 caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag ggagcgataa
    31381 aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg
    31441 taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt gccagctcca
    31501 gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctcgg
    31561 catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt
    31621 taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct
    31681 gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa
    31741 ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca tctggtgcag
    31801 cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg
    31861 agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag
    31921 tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca
    31981 gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaag
//
LOCUS       DAJTVE010000037        29940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000037 DAJTVE010000000
VERSION     DAJTVE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO439_001627"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO439_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6316014.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO439_001628"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO439_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6316015.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO439_001629"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO439_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6316016.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO439_001630"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO439_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6316017.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO439_001631"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO439_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6316018.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO439_001632"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO439_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6316019.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO439_001633"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO439_001633"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6316020.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO439_001634"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO439_001634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6316021.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO439_001635"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO439_001635"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6316022.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO439_001636"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO439_001636"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6316023.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO439_001637"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO439_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6316024.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO439_001638"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO439_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6316025.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO439_001639"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO439_001639"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6316026.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO439_001640"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO439_001640"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6316027.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO439_001641"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO439_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6316028.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO439_001642"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO439_001642"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6316029.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO439_001643"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO439_001643"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6316030.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20761..21432
                     /gene="nfi"
                     /locus_tag="OO439_001644"
     CDS             20761..21432
                     /gene="nfi"
                     /locus_tag="OO439_001644"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6316031.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21475..22065
                     /locus_tag="OO439_001645"
     CDS             21475..22065
                     /locus_tag="OO439_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6316032.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22252..22524
                     /gene="hupA"
                     /locus_tag="OO439_001646"
     CDS             22252..22524
                     /gene="hupA"
                     /locus_tag="OO439_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6316033.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22537..23232
                     /locus_tag="OO439_001647"
     CDS             22537..23232
                     /locus_tag="OO439_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6316034.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO439_001648"
     CDS             complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO439_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6316035.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            23897..25273
                     /gene="zraS"
                     /locus_tag="OO439_001649"
     CDS             23897..25273
                     /gene="zraS"
                     /locus_tag="OO439_001649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6316036.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25270..26595
                     /gene="zraR"
                     /locus_tag="OO439_001650"
     CDS             25270..26595
                     /gene="zraR"
                     /locus_tag="OO439_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6316037.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO439_001651"
     CDS             complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO439_001651"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6316038.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO439_001652"
     CDS             complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO439_001652"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6316039.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7015 a         7803 c         8245 g         6877 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgcta cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacata
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgcttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gtaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaatccc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgactttttg gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggagctggtg
     4861 ccggaacgcc tgcgtggtga aactgcatct ttcgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgttg
     5101 gctaagctga gccagtctgg tcacaagcgt attgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttctg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggacatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccgg gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcattacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctttgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaggtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tttctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgtgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtttgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggtg tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cactggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag tggttccaga
    13681 tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gttctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag
    13861 tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc
    14881 gaacagtcgg agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtaccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga cctgcgattt
    15301 cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cccgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtccaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat
    15721 gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca ttgcgccgat ctttgatgcg tagctggagg gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat
    16201 ttctccgcct ctggcgcgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcacgta cttcctggct gattttcatc gagcagaatt tcggcccaca
    16321 catggagcaa aaatgggcga ctttacctga ctcttgcggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcctgga tgccctttcg ccaggtcagc
    16501 ggcgtggacg gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca
    16801 ggcaattttg gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagtcaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt agtacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgaggcgt acaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatgac ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacctacat ggtggaaggc
    20161 ggcagcagca aagcgttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctac tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gtgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcg
    20461 atggcagaaa ccggttgcga tgcgttgggc ctcgactgga caacggatat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacaat atcaccgcta aggagtgatt
    20761 atggatctcg cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatccgcgag
    20821 gatcgactcg ataaagatcc accggatctg atcgccggag tcgatgtcgg gtttgagcag
    20881 ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg
    20941 gagtataaag ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc
    21001 cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg
    21061 tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtcgctag ccattttggt
    21121 ttgatggtgg atgtgccaac gatcggcgtg gcgaaaaaac ggctctgcgg taaattcgaa
    21181 ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc
    21241 tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc
    21301 agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag
    21361 ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca
    21421 aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta
    21481 caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg
    21541 gcttgcttat gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt
    21601 ggtgatgggc aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa
    21661 gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc
    21721 gacgatttcg atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa
    21781 ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc
    21841 tccatcacga ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa
    21901 gagcttaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga
    21961 cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg
    22021 cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga
    22081 tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt
    22141 tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt
    22201 tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag
    22261 actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaactca ggctaaagct
    22321 gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa
    22381 ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg ccgcaacccg
    22441 cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag
    22501 gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg
    22561 gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggctggcgc tctgcttctc
    22621 actgcctgta gtcataactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac
    22681 cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg
    22741 tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac
    22801 ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaatattcg cgcgcgtttt
    22861 gacgatcgcg gtgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt
    22921 tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat
    22981 gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc
    23041 gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat
    23101 atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc
    23161 gaaggttcgc aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag
    23221 aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt
    23281 agcccatgcc cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt
    23341 tcacccgtaa ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa
    23401 ttttgctgct atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca
    23461 ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg
    23521 ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt
    23581 gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa
    23641 ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc
    23701 ttccgtgcca acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa
    23761 atgactcgcc tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt
    23821 tacagcgcag ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga
    23881 gcaggcaaag aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt
    23941 aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat
    24001 tcgcgattat gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt
    24061 acttattcgc gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc
    24121 gcagcagcag gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt
    24181 cacggatgaa caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct
    24241 ttattccccg caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat
    24301 cgatagcgca gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc
    24361 tgctggaatg caccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt
    24421 catcgctttc gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct
    24481 gattatcctc ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta
    24541 tcgccgctat ttgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct
    24601 ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc
    24661 gattaaagga ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca
    24721 actggcgcag gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagctgct
    24781 ggaactggtt aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa
    24841 ccactcatta cagctggtaa gccaggatgc gaacagccgg gagatccagt tacgctttac
    24901 cgccaacgac acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt
    24961 gaatctctat ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc
    25021 cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc
    25081 agatcagctt gatgccatct tcactccgta cttcaccact aaagccgaag gcaccggact
    25141 ggggctggcg gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag
    25201 ccaggaggga aaaggcgcaa cgttcaccct ctggcttccg gtcactatta cgcgtaaggg
    25261 cccacaagga tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac
    25321 tgcactattt tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc
    25381 gggcgacagg cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg
    25441 cgaatggcgg agatggacgg cattgccacg ctaaaagaga tcaaagcgtt aaatccggcg
    25501 attccggtgc tgattatgac cgcatactcc agcgtcgaga cggcggtaga agcgttgaaa
    25561 accggggcgc tggattatct catcaagccg ctggatttcg ataacctgca ggcgacgctg
    25621 gaaaaggcgc tcgcgcatac gcacagtatt gacgctgaaa cgcctgcggt gtctgccagc
    25681 cagttcggta tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc
    25741 gtcgcgccat cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg
    25801 gtcgccaggg cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac
    25861 tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg
    25921 ttcactggag ccgatagacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg
    25981 tttctcgatg aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt
    26041 caggagcgcg aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggttg
    26101 attgcggcga cccatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat
    26161 ctctactatc gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa
    26221 gatatccctc tgctggctgt ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg
    26281 gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat
    26341 attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt
    26401 tccgaacgcg agctgccgct ggcgattgcc ggtacgccga tcccactggg gcaaagtcag
    26461 gatattcagc cgctggtgga agtcgaaaaa gaagtgattc tggcggcact ggagaaaacg
    26521 ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca
    26581 aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc agccgatatc tttccggcag
    26641 aagcagtcgt cccagtgaat atcggtcatt aaggcataag cgcgtttctg cgcttctgcc
    26701 acggtatgac ccagcgcggt gacgcacagt acgcgcccgc cgctggtcac tacctgctcg
    26761 ttatccgcca gtttcgtgcc cgcgtggaac actttgccgc ctgccacttc ttccagcggc
    26821 aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc
    26881 acgccgagag aagcacgctc atcccactct gacgttttct cgtccagttt gccttcacag
    26941 gccgccaggc agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttct
    27001 ggatcgccaa agcggcagtt aaattcgata accttcggat tgccctgttt gtcgatcatc
    27061 aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt
    27121 ggccagatga tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg
    27181 gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt tatcgcctac gcgtttgtga
    27241 tcctggctgg tggccatcgg cagcacatgc tcgccgtcta ccatcacgat aaagctcgct
    27301 tcttcgccgt cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg
    27361 cccgccagca tatcgtgaac agccgcttcg gcttcttcca gcgtcatcgc cacgataacg
    27421 cctttcccgg ctgccagacc gtccgcttta atgacgatcg gcgcaccttt ctcacgcaga
    27481 tacgccagcg caggttctac ctcggtgaag ttctggtatt ccgctgtagg gatcttatgg
    27541 cgggccagga aatctttggt aaacgctttc gaaccttcca gttgcgcggc acctgcggtt
    27601 gggccgaaga ttttcatccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc
    27661 gcttccgggc cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc
    27721 gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca
    27781 ttacccggag caacaaaaac agtttcaacc agcggcgact gcgccgcttt ccaggccagc
    27841 gcgtgctcgc gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc
    27901 ggaagtggcg catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca
    27961 cttcgtcatc acggatagaa ccgccaggct ggattacgca ggtcacgcct gcagcggcgg
    28021 cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca
    28081 cttccaagcc ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca
    28141 tctggcccgc gccaatcccg atggtcatat tgtttttggc atagacgata gcgttggatt
    28201 tcacgaactt cgctactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct
    28261 gacgttgggt gacgacgcgc agttcttccg caccgaccat gcccaggtca cgatcctgaa
    28321 ccagcagacc gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac
    28381 cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg
    28441 cggaaggcgc aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg
    28501 catccagctc gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt
    28561 acgcgcgatc gtaagcatca agaatggaat tgccgatagc cacgccgcaa gggttggcgt
    28621 gcttcacaat cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg
    28681 tatcggcgat gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg
    28741 aggcttcttt cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt
    28801 aacgcatatc ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg
    28861 cttctttgct ttcaccgtgg taagccggaa ccatgctgcc gaagtagttg gcaatcatgc
    28921 tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg
    28981 tcagcgatcc ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca
    29041 ccacgattgc gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga
    29101 tatcgatgtt ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga
    29161 acggatacag gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg
    29221 catcgtcctg gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca
    29281 cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac
    29341 ctttttctgc taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg
    29401 aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc
    29461 ggactggacg acgttgttgc atggtaaatc ccctggattt gactattaca gagagcgtta
    29521 gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc
    29581 cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca
    29641 caattttgac agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa
    29701 ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt
    29761 ttctgcaatt tttctattgc ggcctgcgga gaaatcccta taatgcgcct ccatcgacac
    29821 ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat
    29881 tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJTVE010000038        29530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000038 DAJTVE010000000
VERSION     DAJTVE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..681
                     /gene="dgt"
                     /locus_tag="OO439_001653"
     CDS             <1..681
                     /gene="dgt"
                     /locus_tag="OO439_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6316040.1"
                     /translation="AVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNS
                     LSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKL
                     YKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPI
                     ESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEY
                     RRLMAVEQ"
     gene            811..2235
                     /gene="degP"
                     /locus_tag="OO439_001654"
     CDS             811..2235
                     /gene="degP"
                     /locus_tag="OO439_001654"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6316041.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2390..3547
                     /gene="cdaR"
                     /locus_tag="OO439_001655"
     CDS             2390..3547
                     /gene="cdaR"
                     /locus_tag="OO439_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6316042.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3636..4022)
                     /locus_tag="OO439_001656"
     CDS             complement(3636..4022)
                     /locus_tag="OO439_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6316043.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4184..4996)
                     /gene="yaeI"
                     /locus_tag="OO439_001657"
     CDS             complement(4184..4996)
                     /gene="yaeI"
                     /locus_tag="OO439_001657"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6316044.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5051..5875)
                     /gene="dapD"
                     /locus_tag="OO439_001658"
     CDS             complement(5051..5875)
                     /gene="dapD"
                     /locus_tag="OO439_001658"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6316045.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5906..8578)
                     /gene="glnD"
                     /locus_tag="OO439_001659"
     CDS             complement(5906..8578)
                     /gene="glnD"
                     /locus_tag="OO439_001659"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6316046.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8640..9434)
                     /gene="map"
                     /locus_tag="OO439_001660"
     CDS             complement(8640..9434)
                     /gene="map"
                     /locus_tag="OO439_001660"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6316047.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9802..10527
                     /gene="rpsB"
                     /locus_tag="OO439_001661"
     CDS             9802..10527
                     /gene="rpsB"
                     /locus_tag="OO439_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6316048.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10785..11636
                     /gene="tsf"
                     /locus_tag="OO439_001662"
     CDS             10785..11636
                     /gene="tsf"
                     /locus_tag="OO439_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6316049.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11783..12508
                     /gene="pyrH"
                     /locus_tag="OO439_001663"
     CDS             11783..12508
                     /gene="pyrH"
                     /locus_tag="OO439_001663"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6316050.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12800..13357
                     /gene="frr"
                     /locus_tag="OO439_001664"
     CDS             12800..13357
                     /gene="frr"
                     /locus_tag="OO439_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6316051.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13449..14645
                     /gene="ispC"
                     /locus_tag="OO439_001665"
     CDS             13449..14645
                     /gene="ispC"
                     /locus_tag="OO439_001665"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6316052.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14834..15592
                     /gene="ispU"
                     /locus_tag="OO439_001666"
                     /gene_synonym="uppS"
     CDS             14834..15592
                     /gene="ispU"
                     /locus_tag="OO439_001666"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6316053.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15605..16462
                     /gene="cdsA"
                     /locus_tag="OO439_001667"
     CDS             15605..16462
                     /gene="cdsA"
                     /locus_tag="OO439_001667"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6316054.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16474..17826
                     /gene="rseP"
                     /locus_tag="OO439_001668"
     CDS             16474..17826
                     /gene="rseP"
                     /locus_tag="OO439_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6316055.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17856..20288
                     /gene="bamA"
                     /locus_tag="OO439_001669"
     CDS             17856..20288
                     /gene="bamA"
                     /locus_tag="OO439_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6316056.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20410..20895
                     /gene="skp"
                     /locus_tag="OO439_001670"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20410..20895
                     /gene="skp"
                     /locus_tag="OO439_001670"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6316057.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20899..21924
                     /gene="lpxD"
                     /locus_tag="OO439_001671"
     CDS             20899..21924
                     /gene="lpxD"
                     /locus_tag="OO439_001671"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6316058.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22029..22484
                     /gene="fabZ"
                     /locus_tag="OO439_001672"
     CDS             22029..22484
                     /gene="fabZ"
                     /locus_tag="OO439_001672"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6316059.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22488..23276
                     /gene="lpxA"
                     /locus_tag="OO439_001673"
     CDS             22488..23276
                     /gene="lpxA"
                     /locus_tag="OO439_001673"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6316060.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23276..24424
                     /gene="lpxB"
                     /locus_tag="OO439_001674"
     CDS             23276..24424
                     /gene="lpxB"
                     /locus_tag="OO439_001674"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6316061.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24421..25017
                     /gene="rnhB"
                     /locus_tag="OO439_001675"
     CDS             24421..25017
                     /gene="rnhB"
                     /locus_tag="OO439_001675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6316062.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25054..28536
                     /gene="dnaE"
                     /locus_tag="OO439_001676"
     CDS             25054..28536
                     /gene="dnaE"
                     /locus_tag="OO439_001676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6316063.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEDMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28549..29508
                     /gene="accA"
                     /locus_tag="OO439_001677"
     CDS             28549..29508
                     /gene="accA"
                     /locus_tag="OO439_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6316064.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7155 a         7117 c         8154 g         7104 t
ORIGIN      
        1 gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca cgaggcgtgg
       61 ggtcagcatg agaaaggttc gctcttttcg ctagtggttg aaaatgcctg ggaaaaatca
      121 cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt acgggtaaac
      181 accctaaata aactggtacc ctacgcggca caacgattta ttgataatct gcctgcgatt
      241 ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag cgatcttctt
      301 aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt cgagcagctt
      361 gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc tttattaagc
      421 ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg tttccctatt
      481 gagtcgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt cgaggctgtc
      541 agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta ccgttgccgc
      601 ctactgcagg attatatcag cggtatgacc gacctctatg cgtgggatga ataccgacgt
      661 ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat ttttaccttt
      721 tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac acagcaattt
      781 tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc actgagtgca
      841 ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc tgagacttct
      901 tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct
      961 tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg tatgccgcgt
     1021 aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc accgtttcag
     1081 agctctccgt tctgccaggg gggccagggc ggtaatggcg gcggccagca acagaaattc
     1141 atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt caccaacaac
     1201 catgttgttg ataacgcgac ggtgattaaa gtccagctga gcgatggtcg taagttcgac
     1261 gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat ccagaacccg
     1321 aaaaacctga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg tgattacacc
     1381 gtagcgattg gtaacccgtt tggtctgggc gagacggtaa cttccgggat tgtctctgcg
     1441 ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact tcatccagac cgatgcagcg
     1501 atcaaccgtg gtaactccgg tggtgcgctg gttaacctga acggcgaact gatcggtatc
     1561 aacaccgcga tcctcgcacc ggacggcggc aacatcggta tcggttttgc tatcccgagt
     1621 aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag
     1681 ctggggatta tggggactga gctgaactcc gaactggcca aagcgatgaa agttgacgcc
     1741 cagcgcggtg ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa agcgggcatt
     1801 aaagcgggtg atgtgatcac ctcactgaac ggtaagccga tcagcagctt tgccgcactg
     1861 cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt actgcgcgac
     1921 ggtaagcagg ttaacgtgaa cctggaactg cagcagagca gccagaatca ggttgattcc
     1981 agctccatct tcaacggcat tgaaggcgct gagatgagca acaaaggcaa agatcagggc
     2041 gtggtagtga acaacgtgaa aacgggcact ccggctgcgc agatcggcct gaagaaaggt
     2101 gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtg
     2161 ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag caccatctac
     2221 ctgttaatgc agtaatctcc ctcaacccct tcctgaatac gggaaggggt tctccttaca
     2281 atctgtgaac ttcgccacaa ctccatacat cttcatcatc ctttgggcat ttgcacaatg
     2341 ccgtacgtta cgtacttcct tatgctaagc cgtgcataac ggaggactta tggctggctg
     2401 gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca tcatcgatac
     2461 caatatcaac gtaatggatg cccgcgggcg aattatcggc agcggcgatc gtgagcgtat
     2521 tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg tcgatatcga
     2581 tgacgcggta gcacgtcatc tgcacggtgt gcggcagggg attaatctac cgttacggct
     2641 ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc tgcgtaaata
     2701 tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt tgatgcacct
     2761 gttggcgcag gatagccgtt tgcgggaaga actggtgatg aacctgattc aggctgagga
     2821 gaatactccc gcacttactg aatgggcgca acggctgggg attgatctca atcaaccgcg
     2881 agtggtggct attgttgagg tcgacagcgg tcagcttggc gtggacagcg caatggcgga
     2941 gttacaacaa ctgcaaaatg cgctgactac gcccgagcgt aataatctgg tggcaattgt
     3001 ttcgctgacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc
     3061 agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt acggccagct
     3121 gcgttttcgc gtttcactgg gcaactattt taccggtcct ggcagtattg cccgatccta
     3181 tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa gtcgctgcta
     3241 tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg actggcaggc
     3301 caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataatg gcttgctgcg
     3361 acgaacgctg gcggcgtggt ttcgccacaa tgtgcaaccg ctggctacgt caaaggcgct
     3421 gtttattcat cgtaatactc tggagtatcg gcttaatcgt atatcggaac tgaccgggct
     3481 tgatttgggt aattttgatg acaggttgct gctgtatgtg gcgttacaac tggatgaaga
     3541 gcggtaggtt atgcgttaag gtcgaggcgg cgctggctca ttcccctcag cccgaagggt
     3601 agaggggaat gtccgagtta accgtcagtt cggcattatt tacgcgttag tttttccaga
     3661 tcggcttcaa tctcgctgat cttattggtt acgactgact ccaggtgacg taagtcgtcg
     3721 aggatcttac gcttaagatc aacttcgctg cggtcacgct ggcagatttg atcaagctca
     3781 tcaatgatat accgtagatt cgggctgatt tcctggactt ctttataacc ctgacccaca
     3841 ccatcagcga cgaccgtttt acgctgacgc ggatatttaa acttcacgct cttggcgaaa
     3901 aactcgcctt tgtctttctg gaaatagatt ttcagaatat cgttgttggc ttcctgccgg
     3961 aggctgtaac gatcaatttc ttcaggattg gtaataccca gacttttcag attgtcgtac
     4021 atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt ttagcatttg gcatggcccg
     4081 cccggctcga ctgtgatcgc agaaagcctt tttcttccag acttctatca tggcgtaaaa
     4141 atcaaaaaat tacctgcttt attgtggtga taaaattcac gttctacacc agttccagca
     4201 tcgttacttc cgggcggcag ttcagacgca aaccatacaa actgcccacg ccacgggttg
     4261 tgtagatgtg tcgttcgcca aaggcattta atccggcgac gtaacgttta tcttcgacag
     4321 gggcaaaagg ttcaccaacc aacggtacgc gcagttgccc gccgtgagta tggccgcaca
     4381 gcatcagatc ccagggttca tcacgcatga cttctttgct gtcgggatta tgtgccagca
     4441 tcagtcttgg cagatttgct tcgctggcgg gaggtggttt gcattgtccg gcccataagt
     4501 caccggtgcc taccagttcg aattgcctgt tcggcgtggc gatcaccgta gcctggttaa
     4561 acaacaccgt gatgcccact gatttcaacg tctcgccaat taagtgattt ttttctgtac
     4621 caacagggcg atcgtggttg ccgaagcagg caaacgtcgg cgcacattcg gcaagggggg
     4681 agaggacatc actaaacgcc gaaaaattca gcggcatatc aaataatacg taatcgccgc
     4741 ccagtaatat caaatcgggc ttttgttcta tgccaagagc aatcgcgtca gaaatcaggc
     4801 ttaaaggaac aaaacgagag taatggagat cggccagaaa aagaattttg aatggtgctg
     4861 cgttgtcttt aaaaaaggcg aggcggtgac ggattaattc aaaccagcct agctcccagt
     4921 aatgcatata accgaaacct gagctcgtgg ctatcgtcgc ggcagtagcc tgcaacaatc
     4981 ggcggcgtga aatcatcgct catccctgca aaaaaaatcg ggcagcgtcg tgctgcccgt
     5041 gtgcatactt ttagtcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt
     5101 tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca tcttttgacg
     5161 gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg
     5221 tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatggaa atgacggaac
     5281 cttcttcgac aatcacccct tcaaccactt cagagcgtgc gccaatgaag cagttatctt
     5341 caatgatggt tgggttagcc tgcagcggtt ccagcacgcc gccgatgccc acgccaccgg
     5401 aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca
     5461 tggtgccttc atcaacatat gcgccgatgt tgacgtaaga cggcatcagc acggtgttac
     5521 gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct
     5581 ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt
     5641 ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca
     5701 accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcaccggaat
     5761 ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgcg ttggctggcg
     5821 tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta
     5881 aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca
     5941 accgctgatg cacttcctgc tgcaactcgt tattaagtgc acgccggtca gcggtggcaa
     6001 ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa
     6061 ttcccagatc ggcaaaaatt ttcccgactc tcgccagcag tcctggctgg tcgagcgcga
     6121 taagctcaag gaacgatttg cggtcggtat gggttggcaa aaacgtcact tcggtttcaa
     6181 cagtaaagtg acgtagttta gccggttgac gacgcggctg tggtggctgc cagctacttt
     6241 gtgtcagtac ctgctccaga ccaaaccgaa taacctcatg acgatctgcg gatagcgggc
     6301 taccatcggg ttccagcacg atgaaggtat ccatcgccat accgtcgcga gtggtgaaaa
     6361 tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca
     6421 gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc
     6481 taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca
     6541 gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca
     6601 gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc
     6661 gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg
     6721 caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagcgtt tcgttggtgg
     6781 cgcaaatgtc agccactgtc aggcatacca gatagtgcag acgattttcc gtttgaactt
     6841 cttcggcaaa ctgcttgatg acttcagggt cctgaatatc gcggcgttgt gctgtcaccg
     6901 acatcagcaa gtgctggcga accagccagg caaccagctg tgtttcgcgt gagttcagcc
     6961 cgtggagttc tgcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc
     7021 gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc
     7081 gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct
     7141 ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa
     7201 actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc acgctatggc
     7261 gatgcattgg caatagcccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca
     7321 gttttcgtgc ttccggaata ttacacagcg gttgttgcag atggcgacgg gcatggcgta
     7381 actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga
     7441 acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta
     7501 gcgtaccgcg tagctgaaac tcatcgtcga ttggacgtgg tttttcgtcg gcgggaaggg
     7561 cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg
     7621 taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca ccactgtaat
     7681 tcagacgctg agcgacgcta agttggcgat cgaataacag gcgattatcg taacggctga
     7741 cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg tttaattccg
     7801 cccgctccgc tgaggttaag aagccaaacc caaccatttc atccagcgat gttgcgccaa
     7861 aatgacggcg ggccacccat tgcagagtgt ggatatcgcg caagccgcca gggctacttt
     7921 tgatgtctgg ttcaagattg tagctggtgc cgtggtaacg ctgatggcgc tggttctgtt
     7981 cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt
     8041 tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg attaaattgg
     8101 tggcgacggt taaatccgat aacccttcca gcatgcactc ttcaagcgtg cgcacactat
     8161 gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct
     8221 gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca
     8281 gctcgccacg accgtagcca ccgacggcga ccaatgccag gtcggcaatc tggctgaatc
     8341 ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa ctcggtgcgc gcctcaatca
     8401 actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga
     8461 tatgggcttt tatcccaccg acggttaatt catcacgggg ccagacgcat ggattttgcg
     8521 gttgaccggg cagggtgggg agagcggtgt ttgcgtactg ttctggaagg gtattcattg
     8581 tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct
     8641 tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt cagaatttcg
     8701 cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg
     8761 gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt
     8821 ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt tggtttcacg ggagtcatag
     8881 tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca atattcacga
     8941 acgacggaga agccttctgc ttcgataaac ttctgaatcg ccgcaccgat ttcgcgcagg
     9001 ttaatgcctg gtttgaccat gcgcagcgcc aggtacaggc tttcttgcgt gatgcggcac
     9061 agacgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg
     9121 aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagcttc
     9181 gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac ggatttcgga
     9241 tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta
     9301 cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc gatcatctcc
     9361 agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg
     9421 attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag
     9481 tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg
     9541 ccactgaacg ttttctataa tagaaaattc aacgtctgat gctgtacaca gcgccaacaa
     9601 ttattggtgt ccatgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt
     9661 atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca
     9721 catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca taaccccaac
     9781 ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt
     9841 gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt
     9901 gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct
     9961 ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa tccttttcgt tggtactaaa
    10021 cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac
    10081 catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt
    10141 ctgaaagacc tggaaactca gtctcaggac ggtactttcg aaaagctgac caagaaagaa
    10201 gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac
    10261 atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc
    10321 aaagaagcaa acaacctggg tattccggta tttgctatcg ttgataccaa ctctgatccg
    10381 gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac
    10441 ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg
    10501 gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa atagttcgag
    10561 ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag tgaccgggat
    10621 gagcgagcga agacaacgca tctgcggcgc gaaatatgaa gggggagagc ccttatagac
    10681 caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac ttttatatct
    10741 gtgcggttta atgccgggca gatcacatct ccgaggattt tagaatggct gaaattaccg
    10801 catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag
    10861 cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt aagtccggtg
    10921 ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa
    10981 tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc gttgcaaaag
    11041 acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct ggcaaaatca
    11101 ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa
    11161 ttggtgagaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt
    11221 atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg
    11281 ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa ccagaagacg
    11341 tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg atgcagtctg
    11401 gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg
    11461 aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc
    11521 tgaaagagca taacgctgaa gtgactggct tcatccgctt cgaagtgggg gaaggcatcg
    11581 agaaagttga gactgacttt gcagcagaag ttgctgcgat gtccaagcag tcttaattat
    11641 cgaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat tcagctaacc
    11701 cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc atttcaatcg
    11761 ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata aacgcattct
    11821 gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg atgcaagcat
    11881 actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc aggttggtgt
    11941 ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg
    12001 cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcctgg caatgcgtga
    12061 tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat tgaatggcgt
    12121 gtgcgacagc tacagctggg cagaagctat cagcctgttg cgcaacaacc gtgtggtgat
    12181 cctctccgcc ggtacaggta acccgttctt taccaccgac tcagcagctt gcctgcgtgg
    12241 tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc
    12301 tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct
    12361 ggaaaaagag ctgaaagtca tggacctggc ggccttcacg ctggctcgtg accataaatt
    12421 accgattcgt gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga
    12481 aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg gtaagattcc
    12541 gcgtaagtat cgcgggggcg taagtctggt tataaggcgt tattgttgca ggcagtttgg
    12601 tcacggccag cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc gagcactgct
    12661 cggggccaaa atggcaaata aaatagccta ataatccaga cgattacccg taatatgttt
    12721 aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg actgagacaa
    12781 gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag tacgcatgga
    12841 caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc
    12901 cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct
    12961 ggcaagcgta acggtagaag attcccgtac tctgaaaatc aacgtgtttg atcgttcaat
    13021 gtctccggcc gttgaaaaag cgattatggc gtccgatctt ggcctgaacc cgaactctgc
    13081 gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta aagatctgac
    13141 caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaacg tgcgtcgtga
    13201 cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag acgacgatcg
    13261 ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa ttgaagcggc
    13321 gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac aaaaacgccg
    13381 ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt ctcaactctg
    13441 gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt tgcagcacgc
    13501 tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa
    13561 atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc gtaatggacg
    13621 atgaagcgag tgcgaaactt cttaaaacga tgctacagca acagggtagc cgcaccgaag
    13681 tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggatgtt gatcaggtga
    13741 tggcagccat tgttggcgct gctgggctgt tacctacgct tgctgcgatc cgcgcgggta
    13801 aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg tttatggacg
    13861 ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac gccatttttc
    13921 agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag caaaatggcg
    13981 tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca ttgcgcgatt
    14041 tggcaacaat gacgcccgat caagcctgcc gtcatccgaa ctggtcgatg ggacgtaaaa
    14101 tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa gcgcgttggc
    14161 tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca gtgattcact
    14221 caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg gatatgcgta
    14281 cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg
    14341 atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgatcgt tatccatgcc
    14401 tgaaactggc gatggaggcg ttcgaacaag gtcaggcagc gacgacagca ttgaatgccg
    14461 caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg gatatcgctg
    14521 cgttgaattt atctgtactg gaaaaaatgg atatgcgcga accacaatgt gtggacgatg
    14581 tgttatctgt tgatgcgaac gcgcgtgaag tcgccagaaa agaggtgatg cgtctcgcaa
    14641 gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc ttcagtgata
    14701 tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg ccgtggtttt
    14761 acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa
    14821 taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc cagcgcatgg
    14881 ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa agcaagggag
    14941 gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg tctcttttgc
    15001 ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa actggaaccg
    15061 accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg atagcgaagt
    15121 aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca gtcgctttaa
    15181 ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagctggga ataccggtct
    15241 gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg gagtcaggca
    15301 actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg aagagatgct
    15361 aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta ggactggggg
    15421 ggagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac tttactttac
    15481 agatgttctc tggcctgatt tcgatgaaca agactttgaa ggggcgttaa atgcctttgc
    15541 taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct gatggggggc
    15601 gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc
    15661 gttgtttctg ttgccgccgg tggggttcgc catcgtaacg ctggtggtct gcatgctggc
    15721 agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag tatggttggc
    15781 ggtgttatgc gggttattgt tggcgctgat gctttttctg ttgccggaat atcaccgaaa
    15841 tattcatcaa ccgctggttg aaatctcact ttgggcttcg ttgggttggt ggattgtcgc
    15901 gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact ctaaaacatt
    15961 gcgccttatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc tggcgttacg
    16021 ggcctggcac tatgacgaga atcattacag tggcgcaata tggctgctct atgtcatgat
    16081 cctggtatgg ggcgctgact ccggcgcata tatgtttggc aaattgtttg gtaaacataa
    16141 gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttataggtg gactagcgac
    16201 cgcagcggta atctcatggg gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac
    16261 cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag
    16321 tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag gacacggtgg
    16381 tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt
    16441 actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg ggatttggct
    16501 tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca tttctgggtt
    16561 gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg
    16621 cggcgaactg ataagctcgg caccgaatat gttatggccc tgattccgct gggcggttat
    16681 gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca ccatgccttc
    16741 aataataaat ctgttggcca acgagcggcg attattgccg caggtccggt tgcaaacttc
    16801 atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg cgtacgtccg
    16861 gtggttggtg aaatagcagc caattcgata gctgcggaag cacaaattgc accaggtacg
    16921 gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg tttgcagttg
    16981 gtcgataaaa ttggcgatga aagcaccacc attacagtag cgccatttgg cagcgaccaa
    17041 cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa agaagatccg
    17101 gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg
    17161 cagccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc
    17221 gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga taacccgggt
    17281 aaatccttag cgttagaaat cgaaaggcag gggagtccct tgtctttgac attaatcccg
    17341 gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt
    17401 cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa
    17461 gccacggaca aaacgtggca gctgatgaag ctgacggtca gtatgctggg aaaattgatc
    17521 accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa gggggctggg
    17581 atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat tagcgtgaac
    17641 ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct gctgttcctt
    17701 gcgatcgaaa agatcaaggg cggaccggta tccgagcggg ttcaagactt ttgttatcgc
    17761 attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga tttctctcgg
    17821 ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag ttgctcatag
    17881 cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta gtgaaagata
    17941 ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc actcctcagt atgccggtgc
    18001 gcacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct ctgtttgcta
    18061 ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg gttcaggtaa
    18121 aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg aaagatgaca
    18181 tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca
    18241 ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt aaatatagcg
    18301 ccagcgtaaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta aaactggtgt
    18361 tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac catgctttca
    18421 ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg tggaacgtgg
    18481 taggcgatcg taaataccag aaacagaaac tggcgggcga ccttgaaacc ctgcgcagct
    18541 actatctgga tcgcggttat gcccgtttca acatcgactc tacccaggtc agtctgacgc
    18601 cagataaaaa aggtatttac gtcacggtga acattaccga aggcgatcag tacaagcttt
    18661 ctggcgttga agtgagcggc aaccttgccg ggcactccgc tgaaattgag cagctgacta
    18721 agatcgagcc gggcgagctg tataacggca ccaaagtgac caagatggaa gatgacatca
    18781 aaaagcttct cggtcgctat ggttatgcct atccgcgcgt acagtcgatg cctgaaatta
    18841 acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac cgtttctacg
    18901 tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa
    18961 tgcgtcagat ggaaggtgca tggctgggaa gcgatctggt cgatcagggt aaggagcgtc
    19021 tgaatcgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc
    19081 cggaccaggt agatgtagtc tacaaggtga aagaacgtaa caccggtagc ttcaacttcg
    19141 ggattggtta cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa caggataact
    19201 ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac cagacctatg
    19261 ctgaattgtc ggtaaccaac ccgtacttca ctgtagatgg tgtaagcctc ggtggtcgtc
    19321 tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc aacaagagtt
    19381 atggtacaga cgtgacgttg ggcttcccga tcaacgaata taactcgctg cgtgcaggtc
    19441 tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc
    19501 tgtactctat gggtgaacat ccgagcacct ctgatcagga taacagcttc aaaacggacg
    19561 acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac ttcccgacag
    19621 atggttcacg tgtcaacctg accggtaaag tgaccattcc tggatcggat aacgaatact
    19681 acaaagtgac gttagacacg gcgacttatg tgccgatcga tgacgatcac aaatgggttg
    19741 ttctggggcg tacccgctgg ggttatggtg atggtttagg cggcaaagag atgccgttct
    19801 acgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc aatacaattg
    19861 gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac tatgattacg
    19921 aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta
    19981 acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt agcgataagt
    20041 atgctaactc ggttcgtact tccttcttct gggatatggg taccgtttgg gatacaaact
    20101 gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat atccgtatgt
    20161 ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc tcctacgccc
    20221 agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac atcggtaaaa
    20281 cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact ttaggcgatc
    20341 aatataagat cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag
    20401 gagtttattg tgaaaaagtg gttattagcg gcaggtctcg gtttagcact ggcaacttct
    20461 gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg
    20521 cagaaaaccg gtgtttctaa cacgctggaa aatgagttca aaggccgtgc cagcgaactg
    20581 cagcgtatgg aaaccgatct gcaggctaaa atgaaaaagc tgcagtccat gaaagcgggc
    20641 agcgatcgca ctaagctgga aaaagacgtg atggctcagc gccagacttt tgctcagaaa
    20701 gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt
    20761 actcgtatcc agactgctgt gaaatccgtt gccaacagcc aggatatcga tctggttgtt
    20821 gatgcaaacg ccgttgctta caacagcagc gatgtaaaag acatcactgc cgacgtactg
    20881 aaacaggtta aataagtaat gccttcaatt cgactggctg atttagcgca gcagttggat
    20941 gcagaactac acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca
    21001 caaacaggtc acattacgtt catggttaac ccaaaatacc gtgagcattt aggcttgtgc
    21061 caggcgtccg cggttgtcat gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg
    21121 gtagtgaaga atccctacct gacttacgcg cgcatggcgc aaattttaga taccacgccg
    21181 cagcccgcgc agaacattgc acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac
    21241 aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg cgataacgtg
    21301 attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc
    21361 tgggcgaacg taaccattta ccatgagatc cagatcggtc agaattgcct gatccagtcc
    21421 ggaacagtgg taggcgcaga cggctttggt tatgccaacg atcgtggtaa ctgggtgaag
    21481 atcccacaga ttggtcgcgt aattattggc gatcgcgtgg agatcggtgc ctgcacaacc
    21541 atcgatcgcg gcgcgctgga tgacactgtt attggcaatg gcgtgatcat tgataaccag
    21601 tgccagattg cacataacgt cgtgattggc gacaatacgg cggttgccgg tggcgtcatt
    21661 atggcgggca gcctgaaaat tggtcgttac tgcatgatcg gcggagccag cgtaatcaac
    21721 gggcatatgg aaatatgcga caaagtgacg gttacgggca tgggtatggt gatgcgtccc
    21781 atcactgaac caggcgtcta ttcctcaggc attccgctgc aacccaacaa agtctggcgc
    21841 aaaaccgctg cactggtgat gaacattgat gacatgagca agcgtctgaa atcgcttgag
    21901 cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt cgccaaactt tacggcctgt
    21961 ctcattctta cgattgcggc aggccgtgtt attattgtcg tttcttatat tttgacagga
    22021 agagtatctt gactactaac actcatactc tgcagattga agagatttta gaacttctgc
    22081 cgcaccgttt cccgttctta ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc
    22141 tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt ccagggccat ttccctggaa
    22201 aaccgatttt cccgggtgtg ctgattctgg aagcaatggc acaggcaaca ggtattctgg
    22261 cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta ctacttcgct ggtattgacg
    22321 aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg
    22381 aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc tctggtcgat ggtaaagtag
    22441 tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg attgataaat
    22501 ccgcctttgt gcatccaacc gccattgtgg aagagggcgc gtcaattggc gcgaacgcac
    22561 acattggtcc tttttgtatc gttggacccc atgtcgaaat tggtgagggt accgtactga
    22621 aatctcacgt tgtcgtgaat ggtcatacta aaattggccg cgataatgag atttatcagt
    22681 tcgcctccat cggcgaagtt aaccaggatc tgaaatatgc tggcgaaccg acccgtgtgg
    22741 aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg
    22801 gcggtggatt gacgaaggtg ggcagcgaca acttactgat gatcaacgcg cacattgcgc
    22861 acgattgtac ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg
    22921 tatcggttga cgacttcgcg atcatcggcg gcatgaccgc agtccatcag ttctgcatca
    22981 ttggtgcgca cgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc cctccttatg
    23041 tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc
    23101 gcggattcag ccgtgaggcg attaccgcta tccgcaatgc gtataagctg atttatcgta
    23161 gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga actggcggaa acatatccgg
    23221 aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg cggtctgatt cgttaatgac
    23281 tgaacagcgt ccattaacga ttgccctggt cgccggagaa acctccggcg atatcctggg
    23341 ggccggttta atccgcgctc tgaaagaaca tgtgcccaac gcccgctttg ttggtgttgc
    23401 cgggccacga atgcaggctg aaggctgcga agcttggtac gaaatggaag aactggcggt
    23461 aatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct
    23521 gacaaagcgt tttggcgaac tgaagccaga tgtttttgtt ggtattgatg cgcccgactt
    23581 caatattact cttgaaggta acctcaaaaa gcagggtatc aaaaccattc attacgtcag
    23641 tccatccgtc tgggcgtggc gacagaaacg cgttttcaaa ataggcagag ccaccgatct
    23701 ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac aaatacaatg taccgtgccg
    23761 ctttatcggt cataccatgg ctgatgccat gccgttagat ccagataaaa atgccgcccg
    23821 tgatgtgctg gggatccctc acgatgccca ctgcctggcg ttgctaccgg ggagccgtgg
    23881 tgcagaagtt gaaatgctta gtgccgattt cctgaaaacg gcccagcttt tgcgccagac
    23941 atatccggat ctcgaaatcg tggtgccact ggtgaatgcc aaacgccgcg agcagtttga
    24001 acgcatcaaa gctgaagtcg cgccagacct ttcagttcat ttgctggatg ggatgggccg
    24061 tgaggcgatg gtcgccagcg atgcggcgct actggcgtcg ggtacggcag ccctggagtg
    24121 tatgctggcg aaatgcccga tggtggtggg atatcgcatg aagcctttta ccttctggtt
    24181 ggcgaagcgg ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg cgggcagaga
    24241 gttagttaaa gaattattgc aggaagagtg tgagccgcaa aaactggctg cggcgctgtt
    24301 accgctgttg gcgaacggga aaaccagcca cgcgatgcac gataccttcc gtgaactgca
    24361 tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg agttagcaca
    24421 atgatcgaat ttgtttatcc gcacacgcag ctggttgcgg gtgtggatga agtcggacgc
    24481 gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt
    24541 gccgggctga atgattccaa aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag
    24601 atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg aaccccacga aatcgacgag
    24661 ctgaacattc ttcatgcgac catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt
    24721 gcgccggaat atgtgttgat tgatggtaac cgctgcccga aattaccgat gcctgcgatg
    24781 gctgtggtga aaggcgatag ccgcgtaccg gaaatcagtg ccgcgtctat cctggcgaaa
    24841 gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt tcccgcaata tggttttgcc
    24901 caacacaaag ggtacccaac cgcttttcat ctggaaaaac tggctgaaca cggcgcgacc
    24961 gaacaccatc ggcgcagctt tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc
    25021 ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg
    25081 cgggtgcaca gcgactactc gatgatcgat ggcctggcca aaaccgcacc gttggtaaaa
    25141 aaggcggcgg cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgtggt
    25201 ctggtgaagt tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat
    25261 tttaacgtcc agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg
    25321 aacaataccg gctatcagaa tctgacgttg ctgatctcaa aagcgtatca gcgcgggtac
    25381 ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg aattaaacga agggttgatc
    25441 cttctttccg gcggacgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg
    25501 ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag
    25561 ctgatccgca ccggcaggcc ggacgaagaa agctatctgc acgcggcggt ggaactggcg
    25621 gaagcgcgcg gtttgcccgt cgtggcgacc aacgacgtgc gctttatcga cagcagcgac
    25681 tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa
    25741 cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggatat gtgcgagctg
    25801 tttgccgaca tccccgaagc tcttgccaac accgttgaga tcgccaaacg ttgtaacgta
    25861 accgtgcgtc ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa
    25921 gattatctgg tcaagcgtgc aaaagagggc ctggaagagc gtctggcctt tttattccct
    25981 gacgaggaag aacgtgttaa gcgccgcccg gaatatgacg aacgtctgga gactgaactt
    26041 caggttatca accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag
    26101 tggtcgaaag ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg
    26161 gtggcctacg cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa
    26221 cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag
    26281 aaacgcgatc aggttatcga gcacgtagcg gacatgtacg gtcgtgatgc ggtatcgcag
    26341 atcatcacct tcggtacaat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg
    26401 gggcatccgt acggctttgt cgatcgtatc tcgaaactga tcccgcccga tccggggatg
    26461 acgctggcga aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa
    26521 gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt
    26581 aagcacgccg gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttac
    26641 tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc
    26701 ggactggtga agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg
    26761 gagatgatca acaagcggcg ggcgaagaat ggcgagccgc cgctggatat cgctgcgatc
    26821 ccgctggatg ataagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc
    26881 cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa
    26941 gatatgatcg cactggtggc actgttccgc cccggcccgt tgcagtcagg gatggtggat
    27001 aactttatcg accgtaaaca tggtcgcgaa gagatttcct atccggacgt acagtggcag
    27061 catgaaagcc tgaaaccggt actggagcca acctacggca tcatcctgta tcaggaacag
    27121 gtcatgcaga ttgcgcaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgt
    27181 cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa
    27241 ggtgcagaaa agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag
    27301 aaattcgcgg gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat
    27361 caaacgttat ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc
    27421 gatatggaca acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg
    27481 aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgacggc
    27541 gaaatcgtgt atggtattgg cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc
    27601 atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgtacc
    27661 gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac
    27721 cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca
    27781 gatcaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa
    27841 gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggtg
    27901 ttagatgggg aacgtgaaac gttaggtctg taccttacgg gacaccctat caaccagtat
    27961 ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa
    28021 cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag
    28081 cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg
    28141 ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc
    28201 gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa
    28261 gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg
    28321 gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga agcccaccgc
    28381 tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt
    28441 tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc
    28501 attggttcgg agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat
    28561 ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg
    28621 gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt
    28681 gaaaaaagcg tagaactgac acgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg
    28741 caactggcac gccatccaca gcgtccttat accctggatt acgttcgcct ggcatttgat
    28801 gaatttgacg aactggctgg cgaccgcgcg tatgcagacg ataaagctat cgtcggtggt
    28861 atcgcccgtc ttgacggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc
    28921 aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg
    28981 cgtctgatgc aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg
    29041 ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac
    29101 ctgcgtgaaa tgtctcgcct cggcgtaccg gtagtttgta cggttatcgg tgaaggtggt
    29161 tctggcggtg cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc
    29221 tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg
    29281 ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc
    29341 gactccatca tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggta
    29401 tcgttgaaag cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat
    29461 ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt
    29521 ctgaaaaagg
//
LOCUS       DAJTVE010000039        29251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000039 DAJTVE010000000
VERSION     DAJTVE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..289
                     /locus_tag="OO439_001678"
     CDS             <1..289
                     /locus_tag="OO439_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6316065.1"
                     /translation="VTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDG
                     STSVTPGTETSNIQLADSAEQTVTFKVDYIATGKATSGNVNAVTNFHINYY"
     gene            372..1073
                     /locus_tag="OO439_001679"
     CDS             372..1073
                     /locus_tag="OO439_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6316066.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1098..3697
                     /locus_tag="OO439_001680"
                     /pseudo
     CDS             1098..3697
                     /locus_tag="OO439_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3688..4758
                     /locus_tag="OO439_001681"
     CDS             3688..4758
                     /locus_tag="OO439_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6316067.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4770..5312
                     /locus_tag="OO439_001682"
     CDS             4770..5312
                     /locus_tag="OO439_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6316068.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5320..5835
                     /locus_tag="OO439_001683"
     CDS             5320..5835
                     /locus_tag="OO439_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6316069.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5801..6538
                     /locus_tag="OO439_001684"
     CDS             5801..6538
                     /locus_tag="OO439_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6316070.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6649..7659
                     /gene="pyrD"
                     /locus_tag="OO439_001685"
     CDS             6649..7659
                     /gene="pyrD"
                     /locus_tag="OO439_001685"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6316071.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7833..8375
                     /gene="zapC"
                     /locus_tag="OO439_001686"
     CDS             7833..8375
                     /gene="zapC"
                     /locus_tag="OO439_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6316072.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8372..9481)
                     /gene="ycbX"
                     /locus_tag="OO439_001687"
     CDS             complement(8372..9481)
                     /gene="ycbX"
                     /locus_tag="OO439_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6316073.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9725..11833
                     /gene="rlmKL"
                     /locus_tag="OO439_001688"
     CDS             9725..11833
                     /gene="rlmKL"
                     /locus_tag="OO439_001688"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6316074.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11845..13752
                     /locus_tag="OO439_001689"
     CDS             11845..13752
                     /locus_tag="OO439_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6316075.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13882..15135
                     /gene="pqiA"
                     /locus_tag="OO439_001690"
     CDS             13882..15135
                     /gene="pqiA"
                     /locus_tag="OO439_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6316076.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15140..16780
                     /gene="pqiB"
                     /locus_tag="OO439_001691"
     CDS             15140..16780
                     /gene="pqiB"
                     /locus_tag="OO439_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6316077.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16777..17340
                     /gene="pqiC"
                     /locus_tag="OO439_001692"
     CDS             16777..17340
                     /gene="pqiC"
                     /locus_tag="OO439_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6316078.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17596..17763
                     /gene="rmf"
                     /locus_tag="OO439_001693"
     CDS             17596..17763
                     /gene="rmf"
                     /locus_tag="OO439_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6316079.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17833..18351)
                     /gene="fabA"
                     /locus_tag="OO439_001694"
     CDS             complement(17833..18351)
                     /gene="fabA"
                     /locus_tag="OO439_001694"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6316080.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18420..20180)
                     /locus_tag="OO439_001695"
     CDS             complement(18420..20180)
                     /locus_tag="OO439_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6316081.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20366..20818
                     /gene="matP"
                     /locus_tag="OO439_001696"
     CDS             20366..20818
                     /gene="matP"
                     /locus_tag="OO439_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6316082.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20894..21934)
                     /gene="ompA"
                     /locus_tag="OO439_001697"
     CDS             complement(20894..21934)
                     /gene="ompA"
                     /locus_tag="OO439_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6316083.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22289..22798)
                     /gene="sulA"
                     /locus_tag="OO439_001698"
     CDS             complement(22289..22798)
                     /gene="sulA"
                     /locus_tag="OO439_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6316084.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23017..23646
                     /gene="sxy"
                     /locus_tag="OO439_001699"
     CDS             23017..23646
                     /gene="sxy"
                     /locus_tag="OO439_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6316085.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23609..25771)
                     /gene="yccS"
                     /locus_tag="OO439_001700"
     CDS             complement(23609..25771)
                     /gene="yccS"
                     /locus_tag="OO439_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6316086.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25781..26227)
                     /locus_tag="OO439_001701"
     CDS             complement(25781..26227)
                     /locus_tag="OO439_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6316087.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26350..28404
                     /gene="helD"
                     /locus_tag="OO439_001702"
     CDS             26350..28404
                     /gene="helD"
                     /locus_tag="OO439_001702"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6316088.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28436..28894)
                     /gene="mgsA"
                     /locus_tag="OO439_001703"
     CDS             complement(28436..28894)
                     /gene="mgsA"
                     /locus_tag="OO439_001703"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6316089.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28990..>29251)
                     /locus_tag="OO439_001704"
     CDS             complement(28990..>29251)
                     /locus_tag="OO439_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6316090.1"
                     /translation="ITSTAKTIDYEVEVERYNKSAKRASLPQFATMMADDSTLLSGVS
                     ELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQPSS"
BASE COUNT         7516 a         6918 c         7694 g         7123 t
ORIGIN      
        1 tgttactgta actaaaaatg cgacgtttac ctttagtggt acggctgacg gcgtgcaacc
       61 tacagcattt gccaaccagg ctaccaccga tgcagcgacg aatgttgctc tacaaatgta
      121 tctgccagat ggttctacat cggtaactcc cggtaccgaa acgtcgaata ttcagttagc
      181 ggattctgct gagcaaacag tgacctttaa agttgattat atcgctacgg ggaaagcgac
      241 ttctggtaat gtgaatgcgg taacaaattt ccatattaac tattattaat agaactcatt
      301 aattgtttta ttaattagta cccctctagt gttctggagg ggatattcat attttttaag
      361 agtgactatt tatgaaaact tgcataacaa agggaattgt gaccgtaagt ttaacggcaa
      421 tattactcag ctgctcatcc gcatgggcgg caggtaaagg cgggattgga cttgcagcaa
      481 cacgtcttgt ttattcagaa ggtgaagagc aaatttcact gggtgtacgt aacaccagtc
      541 cggatgttcc ttatcttatt cagtcatggg tgatgacccc agataataaa aaatcagcag
      601 attttattat tacaccaccg ctatttgtgc tgaatccggc taatgagaat ctgttacgca
      661 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
      721 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      781 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      841 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      901 tcattaccct gacagacctg aaaatagggg gaaaggtagt taaaaatcaa atgatttccc
      961 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
     1021 gaaccatcaa tgattacggg gcggaaacgc cacaactcaa ctgtaaatcg taagccgtct
     1081 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
     1141 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
     1201 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
     1261 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
     1321 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
     1381 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
     1441 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
     1501 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
     1561 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
     1621 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1681 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1741 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1801 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1861 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1921 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1981 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     2041 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     2101 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     2161 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     2221 atgaacagca ggagagcaaa tttgcctagg ccacgttgca gtggggcgga ccgtggggaa
     2281 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     2341 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     2401 tggcagacca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     2461 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     2521 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     2581 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     2641 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2701 aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2761 ttaaagatct ctcgctgggg ggttcctgga actacagtaa gtcccgtggt caacctgatg
     2821 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2881 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gcatcgataa
     2941 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     3001 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     3061 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     3121 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     3181 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     3241 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     3301 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     3361 cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc
     3421 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     3481 gctgcgtttt ggcgcgatag cgacgctgga cggcgtacag actaatagcg gcattattga
     3541 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     3601 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3661 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3721 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3781 agcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3841 accctggagg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3901 gaatcgaatg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3961 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     4021 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     4081 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     4141 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     4201 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     4261 aacatacccg caggtgattc ctgcacgact acaggtacac cggttttagt gtattacctg
     4321 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtc
     4381 gatctgggcg acgcatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     4441 agaacggcag aacttgcaat tccagtcagg tgtaacacgg gaaacgcggg gttagttaac
     4501 gtcaacctga gtctgacggc aaccacagac cccagctatc cccaggcgat taagacgtca
     4561 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     4621 ggaacattac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4681 ccagtcagca cgacaggtgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4741 agaataaatt ttgattaacg cgtgaacgta tgaagaaaga aacgatattt cagtgcgtta
     4801 tcttgttctt tagcattctt aacatccatg tcgggatggc tgggcctgaa caagttagta
     4861 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4921 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4981 ctgctgctga tttgaatatc gatatcaccg gttgtgccgc tagtattact ggcgcggacg
     5041 tcctttttag cggcgaggct gacaccctta cgccgacact gctcaaacta actgacacag
     5101 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     5161 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     5221 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     5281 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     5341 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     5401 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     5461 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     5521 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgacgtcacc
     5581 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     5641 gcagccagcg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5701 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5761 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5821 cttcattatg actaatacgt ggaacagatt ggcgctcttg attttcgccg ttttatcgct
     5881 gctggtggcg ggtgaattgc gggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5941 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cctggcttat
     6001 taacagtaaa atcaaccgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     6061 accattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     6121 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     6181 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     6241 ggtatttaaa ctgttctggc gacccgaagg tttgccgggc gacccgctgg aagcttatca
     6301 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     6361 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagttgtgcc
     6421 gcctaaaagc cagcgtcaga caagctggtg tcaggcaatc gcaccgtgtc atgtcgcctg
     6481 gcgggcgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     6541 caggttcgga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact
     6601 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6661 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6721 caattacgcc gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6781 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6841 gataaagacg gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6901 ggtaccgtca cgccacgtcc acagccaggt aatgacaagc cgcgtctctt tcgtctggta
     6961 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     7021 gagaacgtaa aaaaggccca ttatgacggc gtcctgggta ttaacatcgg caaaaataaa
     7081 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     7141 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     7201 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     7261 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     7321 gaagaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     7381 accaatacca cactcgatcg ttctcttgtt cagggaatga aaaattgcga tcaaaccggt
     7441 ggcttaagtg gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcactg
     7501 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     7561 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     7621 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7681 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7741 atttagacat tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt
     7801 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7861 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7921 ttcgctcacg ttttgcgcgc aagatgttga ctccagatgc cttttctccc gcaggctttt
     7981 gcgttgacga cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     8041 ctaaagaaca aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac
     8101 cgcaaatgcc gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg
     8161 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     8221 aatctggcga aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     8281 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta
     8341 atgttgacag cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg
     8401 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggca
     8461 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     8521 aagggatacg aattccctga ttttctaatt gctccagcaa cacctgttgg ttatttccac
     8581 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     8641 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8701 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8761 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8821 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8881 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg
     8941 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     9001 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     9061 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     9121 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     9181 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     9241 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     9301 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     9361 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     9421 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     9481 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     9541 ataatgcgca acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg
     9601 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc
     9661 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9721 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9781 actggaaaac ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9841 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9901 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9961 caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa
    10021 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
    10081 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
    10141 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
    10201 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
    10261 cgctattgtg atgcgaaccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg
    10321 ttccggtacg ttgctgattg aagcggcgat gctagcgacc gatcgcgcac caggcttgca
    10381 ccgtgggcgt tggggcttta gcggctgggt gcagcatgat gaagctatct ggcaggaagt
    10441 gaaagcggaa gcgcaaactc gtgcccgtaa aggcctggct gagtatagct ctcattttta
    10501 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
    10561 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
    10621 gaaagggccg tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga
    10681 accggcgctg attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg
    10741 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc
    10801 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10861 tgccgaaagc actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct
    10921 gcgtaagaac ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg
    10981 cctgtatgac gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    11041 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct
    11101 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    11161 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    11221 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    11281 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    11341 caaagatttc ctcaacctgt tctcttatac cggtagcgcc accgtgcatg cgggattagg
    11401 cggcgcgcgc agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg
    11461 caacctgcgt ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct
    11521 ggcgtggctg cgtgaggcaa atgaacagtt tgatctgatc tttatcgatc cgccaacctt
    11581 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcata tggcgctgat
    11641 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11701 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11761 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11821 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11881 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11941 tgtctggtgg gtcgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct caaccgtgaa
    12001 caagggctgg atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa
    12061 caggagccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    12121 gaacaagcgg aatatctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg
    12181 agcgagaaaa atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg
    12241 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaatgtt
    12301 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    12361 agtaatccgc gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat tgaaaccatc
    12421 gactggctgg aagggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    12481 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    12541 acctatccag ggaattacga ccagtacctg cttgaaaaag aagaagccct gcgcgtggaa
    12601 gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag
    12661 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt
    12721 cgcgagcgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12781 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag
    12841 caactggtga aagatttttc ggcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12901 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcgatcagct tcaagcggac
    12961 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    13021 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    13081 gttaacggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    13141 cgggcgatga cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    13201 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    13261 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    13321 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgttggat cttcgaaggc
    13381 ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagagcag
    13441 tatgtggcgc tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc ggcaaaagca
    13501 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    13561 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaggtggcg
    13621 gatgcttcct tcttcagtca gccacatgag cagacacaaa aagtgctcgc cgatatggct
    13681 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13741 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13801 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagccat ctgtaaaata
    13861 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13921 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcgacatgt
    13981 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tccgaccgcc
    14041 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    14101 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    14161 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    14221 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    14281 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgct
    14341 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    14401 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    14461 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
    14521 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    14581 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagccag
    14641 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14701 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14761 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14821 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14881 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14941 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    15001 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    15061 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    15121 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    15181 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    15241 tttttatcat tacagccatc agggactgga agtgaccctg atcaccgcga atgcggaagg
    15301 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    15361 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gtgcggctga attctggtat
    15421 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    15481 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    15541 gaaaggcagc aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga
    15601 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15661 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15721 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15781 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15841 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15901 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15961 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    16021 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    16081 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    16141 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    16201 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    16261 cggaaacctg gtcactggtg cactgtatgt tgatcttgat ttctatccaa atacgcctgc
    16321 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    16381 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    16441 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    16501 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    16561 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    16621 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16681 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16741 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16801 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16861 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16921 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16981 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acaattgcgc
    17041 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    17101 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    17161 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    17221 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtactg
    17281 gccagtgtct ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccttaa
    17341 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    17401 ctaacgtgcg gaaaaagttg ttcctcttta cattttttgt acaaccgaca tacccgtgta
    17461 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    17521 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    17581 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    17641 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17701 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17761 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17821 gtctgattat aatcagaagg cagaagtatc ctggaacaga ccgactttca ggtcgctggc
    17881 ggtatagatc aaacgaccat caaccagcac ttctccatcc gccaggccca taatcagacg
    17941 acggttaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    18001 ctgaccagtg aatttcactt cgccaacgcc cagtgcgcgg cctttacctt cgccgcccag
    18061 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    18121 aaccggatcg ccaataaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    18181 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    18241 catcatcagc atattcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg
    18301 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    18361 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    18421 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    18481 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    18541 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    18601 atggtgaatt tgccttcttc taccgctttc actagttcac tgtgaagact taaatggcga
    18661 acgttagctg tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata
    18721 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga
    18781 tcgactgaac ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc
    18841 agggcgcaga gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca
    18901 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18961 gcttgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    19021 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    19081 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    19141 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    19201 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    19261 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta
    19321 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag
    19381 gcatccgctc ccggtgcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    19441 cactgggtta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    19501 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    19561 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    19621 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19681 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19741 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19801 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19861 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg accgcccacc
    19921 agttggcctg catcgctttg tagcgtacgc gcggcattgg caataagatt cagatactca
    19981 gactcttccg gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    20041 tccagcgcaa attgcagccg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    20101 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    20161 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    20221 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactccaggc atgggcgtta
    20281 aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga
    20341 gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg
    20401 ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg gagttaatca cccgttacat
    20461 agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt
    20521 gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca
    20581 aaccattcgg gcaagacgaa agcgccattt taatgcggaa caccagcata cgcgcaaaaa
    20641 atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg
    20701 taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata
    20761 cgcgaataaa atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact
    20821 gacgaaagtc agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct
    20881 accagacgag aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga
    20941 tctctacgcg acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt
    21001 cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg
    21061 ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac
    21121 cctggttgta agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt
    21181 ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg
    21241 gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg
    21301 tctgtacttc cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac
    21361 cgaaacggta ggaaaccccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt
    21421 gtgcgtcacc gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga
    21481 tcgcgtactc aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tttttaccat
    21541 aaacgttgga tttagtgtct gcacgccata ccataccacc cagacgagtg tagatgtcca
    21601 ggtcgtcagt gattgggtaa cccagtttag cggtcagttg aacgccctga gctttgtatg
    21661 caccgttttc aacgctgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa
    21721 agccaacata cgggttaacc tggtaaccac caaaagcacc agcgcccagt tggttttcat
    21781 gggtcgggcc attgttgttg atgaaaccag tgtcatggta ctgggaccag cccagtttag
    21841 caccagtgta ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca
    21901 gtgccactgc aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa
    21961 tacgccatga atatctccaa cgagataaca cggttaaatc cttcaccggg ggatctgctc
    22021 aattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    22081 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    22141 aaggtatgtt taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct
    22201 tcagacaagc ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa
    22261 aaagttccag gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc
    22321 cggaaagttg tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag
    22381 cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg
    22441 ccaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca
    22501 cagtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg
    22561 cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt
    22621 gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt
    22681 cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa
    22741 ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa
    22801 tcaatccagc ccctatgagt tactgtatgg atgtacagta catccagtga caacaaagat
    22861 caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat
    22921 taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga
    22981 atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg
    23041 atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt
    23101 ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg
    23161 tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg
    23221 acatataaaa agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta
    23281 tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa
    23341 gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat
    23401 ctggaagcga ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg
    23461 gcaaaaatgt gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg
    23521 tttatgcttg aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc
    23581 caggagcttg cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt
    23641 gagtaatctg gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt
    23701 gttgtacgac cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt
    23761 tcaggctagc taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt
    23821 catcaacata gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct
    23881 cccggtgagc accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc
    23941 gaaacgcggc ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca
    24001 ccgacgccag ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac
    24061 ggttatcacg cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct
    24121 ctgtggcgcg ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc
    24181 tcactgccgc ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta
    24241 cttcaaaacc ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg
    24301 ttgcatgagc gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca
    24361 gcaccagctg cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac
    24421 ctaccagcgt accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct
    24481 ggcagacaaa caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa
    24541 tgatggcgta gccgaagcac aataccagcg acattcttac cgcatgacgg aagagggcgg
    24601 attccggcgt gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt
    24661 catcagcgag ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa
    24721 ttgttgccag ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga
    24781 gtaaatcggc gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa
    24841 aagcgcgctc aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca
    24901 gttgctggca cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc
    24961 tgtggcgaaa atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt
    25021 gaatatcctg tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat
    25081 cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac
    25141 cgttggcgag agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa
    25201 acatgcgcga cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt
    25261 cctgcagcgg gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga
    25321 cggcaccggc cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca
    25381 acatagtgta aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca
    25441 gaccgccgag caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg
    25501 gccagggaaa cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga
    25561 tgaggttacg caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca
    25621 tccccagcgt tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg
    25681 ttccacaaag cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc
    25741 gtttgagcaa aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg
    25801 cattggcttc acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc
    25861 atagtgcaat agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa
    25921 tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca
    25981 gccagaaaat attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt
    26041 tcagttcatc gacatgaata gcttcattgc cataaggcac cagagatagt ttagtgatct
    26101 cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca
    26161 acagccagcc cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg
    26221 tacgcataaa acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt
    26281 ttgggctgga gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc
    26341 tacagagtca tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac
    26401 gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt
    26461 attcctttca atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg
    26521 gaatttgtac tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg
    26581 cagcgttttt accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa
    26641 attgcgtctg gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat
    26701 aaatggctga cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg
    26761 gcgttgccgt tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt
    26821 aaatgtcagg cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat
    26881 accgaagcca tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg
    26941 aatccggcgc aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt
    27001 gcaggaagcg gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt
    27061 gaagcgtccc ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg
    27121 gacgagcgga ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg
    27181 ctggcgctgc atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa
    27241 aatgataccg ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa
    27301 aagaaagcgc aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg
    27361 ccagaaggta acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat
    27421 cgctgggtaa gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca
    27481 cccgaagaga ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa
    27541 gcctggaaag gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcatcag
    27601 gcgattgtga ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat
    27661 gaatttcagg atatctcgcc gcagcgggca gcgttgttag cggcattacg caagcaaaac
    27721 agtcagacga cgttgttcgc tgttggtgat gactggcagg cgatttaccg attcagcggt
    27781 gcgcaaatgt cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat
    27841 ttagacacga cttaccgttt taacagtcgt atcggtaagg tggcaaaccg gtttattcag
    27901 cagaacccag gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc
    27961 gtcacgttat tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc
    28021 aaaccggaag agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg
    28081 gaaaaagcgg caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc
    28141 aaagggcaac aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt
    28201 ccggctgcgg cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc
    28261 ccggacgctg aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta
    28321 tgggcactgt ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat
    28381 gtgccggtgg cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact
    28441 tcagacggtc cgcgagataa cgctgataat cggggattag aatatcgacc gcgtcgttga
    28501 aatgcggcga ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc
    28561 ataccgtcgc cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg
    28621 gatcccagaa gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct
    28681 ggtcaccccc cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa
    28741 ttaagttacc ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc
    28801 gttccaccca gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt
    28861 tccgcgcagg taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct
    28921 acagattact gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc
    28981 acaaaaatct caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg
    29041 tgggtcagcc agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg
    29101 tggaatagca tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt
    29161 ggcaaattgc ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc
    29221 ataatcaatc gtttttgccg ttgaggtaat g
//
LOCUS       DAJTVE010000040        28797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000040 DAJTVE010000000
VERSION     DAJTVE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO439_001705"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO439_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6316091.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO439_001706"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO439_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6316092.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO439_001707"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO439_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6316093.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO439_001708"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO439_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6316094.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO439_001709"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO439_001709"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6316095.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO439_001710"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO439_001710"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6316096.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO439_001711"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO439_001711"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6316097.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OO439_001712"
     CDS             complement(4604..5326)
                     /locus_tag="OO439_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6316098.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO439_001713"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO439_001713"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6316099.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OO439_001714"
     CDS             6526..6744
                     /locus_tag="OO439_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6316100.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO439_001715"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO439_001715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6316101.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OO439_001716"
     CDS             complement(7478..7678)
                     /locus_tag="OO439_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6316102.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO439_001717"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO439_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6316103.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO439_001718"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO439_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6316104.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO439_001719"
     CDS             10175..12088
                     /locus_tag="OO439_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6316105.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO439_001720"
     CDS             12088..13110
                     /locus_tag="OO439_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6316106.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO439_001721"
     CDS             13104..13322
                     /locus_tag="OO439_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6316107.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO439_001722"
     CDS             13376..14245
                     /locus_tag="OO439_001722"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6316108.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OO439_001723"
     CDS             complement(14300..14704)
                     /locus_tag="OO439_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6316109.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OO439_001724"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OO439_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6316110.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OO439_001725"
     CDS             15689..17779
                     /locus_tag="OO439_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6316111.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO439_001726"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO439_001726"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6316112.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO439_001727"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO439_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6316113.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OO439_001728"
     CDS             complement(19747..20349)
                     /locus_tag="OO439_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6316114.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OO439_001729"
     CDS             complement(20339..20506)
                     /locus_tag="OO439_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6316115.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO439_001730"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO439_001730"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6316116.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OO439_001731"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OO439_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6316117.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OO439_001732"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OO439_001732"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6316118.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OO439_001733"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OO439_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6316119.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OO439_001734"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OO439_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6316120.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OO439_001735"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OO439_001735"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6316121.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OO439_001736"
     CDS             28334..28501
                     /locus_tag="OO439_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6316122.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJTVE010000041        28606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000041 DAJTVE010000000
VERSION     DAJTVE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..852)
                     /gene="melB"
                     /locus_tag="OO439_001737"
     CDS             complement(<1..852)
                     /gene="melB"
                     /locus_tag="OO439_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6316123.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLV"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="OO439_001738"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="OO439_001738"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6316124.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="OO439_001739"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="OO439_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6316125.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3700..5967
                     /gene="adiA"
                     /locus_tag="OO439_001740"
     CDS             3700..5967
                     /gene="adiA"
                     /locus_tag="OO439_001740"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6316126.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6292..7053
                     /gene="adiY"
                     /locus_tag="OO439_001741"
     CDS             6292..7053
                     /gene="adiY"
                     /locus_tag="OO439_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6316127.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7190..8527
                     /gene="adiC"
                     /locus_tag="OO439_001742"
     CDS             7190..8527
                     /gene="adiC"
                     /locus_tag="OO439_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6316128.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8631..10274
                     /gene="eptA"
                     /locus_tag="OO439_001743"
     CDS             8631..10274
                     /gene="eptA"
                     /locus_tag="OO439_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6316129.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10271..10939
                     /gene="basR"
                     /locus_tag="OO439_001744"
     CDS             10271..10939
                     /gene="basR"
                     /locus_tag="OO439_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6316130.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10940..12040
                     /gene="pmrB"
                     /locus_tag="OO439_001745"
     CDS             10940..12040
                     /gene="pmrB"
                     /locus_tag="OO439_001745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6316131.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="OO439_001746"
     CDS             complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="OO439_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6316132.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12217..13719)
                     /gene="proP"
                     /locus_tag="OO439_001747"
     CDS             complement(12217..13719)
                     /gene="proP"
                     /locus_tag="OO439_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6316133.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13983..14849)
                     /locus_tag="OO439_001748"
     CDS             complement(13983..14849)
                     /locus_tag="OO439_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6316134.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="OO439_001749"
     CDS             complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="OO439_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6316135.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17487..17822
                     /locus_tag="OO439_001750"
     CDS             17487..17822
                     /locus_tag="OO439_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6316136.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17980..18423
                     /gene="yjdN"
                     /locus_tag="OO439_001751"
                     /pseudo
     CDS             17980..18423
                     /gene="yjdN"
                     /locus_tag="OO439_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18556..19344
                     /gene="phnC"
                     /locus_tag="OO439_001752"
     CDS             18556..19344
                     /gene="phnC"
                     /locus_tag="OO439_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6316137.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19369..20385
                     /gene="phnD"
                     /locus_tag="OO439_001753"
     CDS             19369..20385
                     /gene="phnD"
                     /locus_tag="OO439_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6316138.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20511..21290
                     /gene="phnE"
                     /locus_tag="OO439_001754"
     CDS             20511..21290
                     /gene="phnE"
                     /locus_tag="OO439_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6316139.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21311..22036
                     /gene="phnF"
                     /locus_tag="OO439_001755"
     CDS             21311..22036
                     /gene="phnF"
                     /locus_tag="OO439_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6316140.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22037..22489
                     /gene="phnG"
                     /locus_tag="OO439_001756"
     CDS             22037..22489
                     /gene="phnG"
                     /locus_tag="OO439_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6316141.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22486..23070
                     /gene="phnH"
                     /locus_tag="OO439_001757"
     CDS             22486..23070
                     /gene="phnH"
                     /locus_tag="OO439_001757"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6316142.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23070..24134
                     /gene="phnI"
                     /locus_tag="OO439_001758"
     CDS             23070..24134
                     /gene="phnI"
                     /locus_tag="OO439_001758"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6316143.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24127..24972
                     /gene="phnJ"
                     /locus_tag="OO439_001759"
     CDS             24127..24972
                     /gene="phnJ"
                     /locus_tag="OO439_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6316144.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24969..25727
                     /gene="phnK"
                     /locus_tag="OO439_001760"
     CDS             24969..25727
                     /gene="phnK"
                     /locus_tag="OO439_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6316145.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25838..26518
                     /gene="phnL"
                     /locus_tag="OO439_001761"
     CDS             25838..26518
                     /gene="phnL"
                     /locus_tag="OO439_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6316146.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26515..27651
                     /gene="phnM"
                     /locus_tag="OO439_001762"
     CDS             26515..27651
                     /gene="phnM"
                     /locus_tag="OO439_001762"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6316147.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27651..28208
                     /gene="phnN"
                     /locus_tag="OO439_001763"
     CDS             27651..28208
                     /gene="phnN"
                     /locus_tag="OO439_001763"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6316148.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28195..>28606
                     /gene="phnO"
                     /locus_tag="OO439_001764"
     CDS             28195..>28606
                     /gene="phnO"
                     /locus_tag="OO439_001764"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6316149.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSH"
BASE COUNT         6927 a         7934 c         7383 g         6362 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt accccatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcagat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
     3541 cgataataag cagtgaaaac aatttaaaat aatcacataa cgcattgttt ataaaaataa
     3601 atatttcaaa aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt
     3661 gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa
     3721 agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct
     3781 ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt
     3841 ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga acatccggac
     3901 gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg
     3961 gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag
     4021 cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgct
     4081 gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg
     4141 aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt
     4201 tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgc
     4261 accgatatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt
     4321 ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc
     4381 gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc
     4441 gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct gacaggcgcg
     4501 aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg
     4561 caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct gactaaagac
     4621 aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt
     4681 tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac
     4741 gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc
     4801 ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac ccacaaactg
     4861 ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac
     4921 ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc
     4981 tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgacg
     5041 caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct gtataaagag
     5101 ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca
     5161 caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag
     5221 gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac
     5281 tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg tgaagatggt
     5341 gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc
     5401 attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc
     5461 cgtgggaaat ggggaactct ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc
     5521 aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg
     5581 aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct
     5641 ggcgcacggt tgaacgaagc ctattccggc ctgtcggtgg cggaaatcac cccgcgtgaa
     5701 gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga
     5761 cgcatcgcgg caaactcagt tatcccatat ccgccaggaa tcccgatgct gctgtctggt
     5821 gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg
     5881 gaccaccatt tccctggatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt
     5941 taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat
     6001 aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct
     6061 gcaaacaaaa atgtcatact ttttgcgcgg ccccaccccg cgcttttttt tgcctgttat
     6121 ttatcctgta aaaaaatatg tacatgagaa attactataa aaatttgtaa tattagtaaa
     6181 actcgttatt tttatgcatg tttatattca tcatacaatt atataaccat ttcccggtat
     6241 cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt
     6301 tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat aagggtgaat
     6361 gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat
     6421 attatcgacg tctcctctct taacaacact ttggttgctc atattagtca cgacatcatc
     6481 aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc
     6541 gctacgccca tactcaccct gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa
     6601 cacagcatga tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc attattattc
     6661 acggtgctat cccgttttct cgacagtaaa aaattccttt cattaatgat gtatatgtta
     6721 cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatattca caaagactgg
     6781 aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg
     6841 aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg ttatgccgta
     6901 aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg cggctacaac
     6961 agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacgcc gctgcattat
     7021 gttagtcagc acagagaacg aactgtcgcc tgatttttaa ccttaacgaa gagctatatt
     7081 aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg caggcgtatg
     7141 atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta tgtcttcgga
     7201 tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg ggaatattat
     7261 ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc actggcggga ttgccattta
     7321 tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc
     7381 gttcctcgat ccaagtcctg gtggttctta cgcttacgcc cgccgctgct ttggcccgtt
     7441 tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca atatcgctat
     7501 ggtggtcatt ggcgtaggat atttaagtta cttcttcccg attctgaaag atccattggt
     7561 attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc
     7621 gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg
     7681 gattgccgta tttggctggt tctggttccg tggtgaaacc tatatggcgg catggaacgt
     7741 cagcggcctg ggcaccttcg gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt
     7801 catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt
     7861 ccctatcgcc accattggtg gggtattgat tgccgccgtt tgctatgtgc tctccaccac
     7921 cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga
     7981 tgccgcacgg atggcgttgg gtgacaccgc tggggccatt gtttccttct gcgcagctgc
     8041 gggttgctta ggttcactgg gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc
     8101 tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg gtacgccagt
     8161 ggcggggctg attatcgtcg gtattttgat gaccatcttc cagctcagta gcatttcgcc
     8221 aaacgcaacc aaagagttcg gtctggtatc ttccgtgtcg gtcatcttta cactggtgcc
     8281 atatctttac acctgtgcgg cgttactgct gctcggccac ggtcactttg gtaaagcacg
     8341 cccggcatat ctggctgtta ctaccattgc cttcctctac tgcatctggg ccgtggtggg
     8401 gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtcatca ccgccatgta
     8461 tgcgctgaat tacaaccggc tacataaaaa cccgtatccc ttagatgcac caataagcaa
     8521 agattaattc cccttaatcc agcaaacata aaagccaacc ttaagaactt aaggttggct
     8581 taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc
     8641 gcctactaaa aagcccctct ttgaatttac tcgcctggct attgttggcc gctttttata
     8701 tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg ccgctggatt
     8761 cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt cgctttcagc gtgattaata
     8821 ttgtcctgac actaagctct ttcttatggc ttaatcgacc actggcctgc ctgtttattc
     8881 tggttggcgc ggctgcacaa tatttcataa tgacttacgg catcgtcatc gaccgctcga
     8941 tgattgccaa tattattgat accactccag cagaaagtta tgcgctgatg acaccgcaaa
     9001 tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa
     9061 tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat attctggttt
     9121 ctgtactgct gattttgctg gtcgccgcac tgttttataa agactacgcc tcgttgttcc
     9181 gcaataacaa agagctggtg aaatccttaa gcccctctaa cagcattgtt gccagctggt
     9241 catggtactc ccatcagcga ctggcaaatc taccgctggt gcgaattggt gaagacgccc
     9301 accgcaaccc gttaatgcag aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg
     9361 aaacctcgcg ggcggagaac ttctccctca acggctaccc gcgtgaaact aacccgcggc
     9421 tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg gcaacggcag
     9481 tttcagtacc gtgcatgttc tcggatatgc cgcgtgagca ttacaaagaa gagctggcac
     9541 agcaccagga aggcgtgctg gatatcattc agcaagcggg catcaacgtg ctgtggaatg
     9601 acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc
     9661 tgaatctgcc tggtcagtgc atcaacggcg aatgctatga cgaagtgctg ttccacgggc
     9721 tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac accatcggca
     9781 gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt accccaacct
     9841 gcgacaccaa tgagatccag acctgtacca aagagcaact ggtgaacact tacgacaaca
     9901 cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa gaacatcagg
     9961 ataaatttac caccagcctg gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg
    10021 gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa caggtgccga
    10081 tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag aactgcctgc
    10141 aaaaacaggc gcaaacgcaa cactattcac aagacaattt attctccacg ctattgggat
    10201 taactggcgt tgagacgaag tattaccagg ctgcggatga tgttctgcaa acttgcagga
    10261 gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc agggactgat
    10321 tctggcggcg caaaccgaag gctacacgtg cgatggcgta acaaccgcgc ggatggcgga
    10381 acaaagcctt gaggccggtc attacagcct ggtggtactg gatttagggt tacccgacga
    10441 agatggactg cattttctcg cccgtatccg gcagaaaaaa tacaccctgc cggtactgat
    10501 cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga
    10561 ctatctggtg aagccttttg cgctggaaga gttacacgcc cgtatccgcg ccctgctacg
    10621 acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc tgaatatggg
    10681 tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag aatatgctct
    10741 gctgtcacgg ttaatgctca aagcaggcag tccggtacat cgggaaattc tctacaacga
    10801 catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata tccacaatct
    10861 gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct atatgctggt
    10921 cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg
    10981 ccaacggctg atattgacca tcggggccat tttgttggtg tttgagctga tcagcatctt
    11041 ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa
    11101 tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt
    11161 ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg tacgccgcat
    11221 cacccgcccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac
    11281 gcccattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga
    11341 tctggtcagt cgcctgacga gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc
    11401 gcacgaactg cgaacgccac tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac
    11461 gcatcacatt gatgtagcac cgttagtggc acggcttgat cagatgatgg agagcgtctc
    11521 ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt atcaacatgt
    11581 aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca tgctcgacca
    11641 gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc agggtgatgc
    11701 aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt acagcccaca
    11761 aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg ccgttgaaga
    11821 tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt ttgtacgtat
    11881 ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca ttacacagtt
    11941 gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc gggcctgggt
    12001 acggctgaag aaagatcagt acgtagcaaa ccagatataa agaaagctgc tgaccaccag
    12061 cacactgaac acggtagtta aactttcata aacacggttt ttcattgcac tctcctccgt
    12121 taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt caattggcct
    12181 gataagacag cgtcacatca ggccatccgt ttcagcttat tcatcaattc gcggatgttg
    12241 ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat
    12301 tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct gtatatctga
    12361 cgccgccggt gtcgcacctt tcaacggacg atttgccgtc tctttcatgg ttacgccggt
    12421 gattaaacca atcaccgcca ctaccatcag gtaataggca ggcatcatca gattctgcga
    12481 gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt
    12541 aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga
    12601 agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc cggcaaaaat
    12661 caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca caaacagggc
    12721 aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca agcccatcac
    12781 cggctggaca aacagcatac cgatcataat ggcgataata atcagcaccc cgtggtcttc
    12841 ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca tgtagtaagt
    12901 cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt atttagtggc
    12961 aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag
    13021 tttatcaaca tgctgctgga acgccggagt ttcttccagc gcatggcgca ggtaaagccc
    13081 gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt cgaggaagtt
    13141 cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca caaacccggc
    13201 aatagaaccg aagtccagcc agctgcccat aaagccacgt ttacggtcag gggagtattc
    13261 cgcaacaaat atcgacgccc cggtatattc accgccgacc gagaaacctt gtgccatctt
    13321 acagatcaac agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc
    13381 aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct tctggcgacc
    13441 atatttatcg cccaacatac caaagaagag cccgccaagc ggtcgaatca gaaagggaac
    13501 ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg ggaaaaaaac
    13561 tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcgaaccatt ccattgcatt
    13621 acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa tggtgacatc
    13681 acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg cagagttggc
    13741 ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag cgggaattgc
    13801 aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt taaggaatgt
    13861 acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc ctaccagtgt
    13921 ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat gtaactgatt
    13981 atttacgcca aatttacaga gaagatttcc tcgaccattg cccccaccaa acgctgcgct
    14041 gaacagagtc gtggcatccc tagcgcgact gtttgccagc gttgggtcac tgaccagcaa
    14101 acgggctgtt tatctgccgt ggaccacttt cccagccaac ttaaaaaatc agcatgctcc
    14161 cctcccgaag aggcaaacca ctgatggtaa tagtgacgcg tggtcggtgc cgcattgata
    14221 ctctctgcca gatgttgtgt cgctttctcg cgcaaatcac ccagtaacaa caccaccgct
    14281 tgcgggcttc ccagacggca ggcatcgcca aatgcacccc agcgcaactc ctccagaacg
    14341 ataaaacagc gcccaggcaa cgaccaggcg ttatacgccc cagcacgcca gcgggtaaaa
    14401 atttgctcgc tctgatcacc tgcctgcacc agtaggcctc gttgcgttaa cgccttttcc
    14461 ttccagcgac aacgggctga aaaccactca tacaaggcgg caatttgctg ggggagctcc
    14521 gggaaagcag agtgctggcg gtgatccatt ttttgcagca gcgccagatg ctgcgcgatc
    14581 tgcgtcagcg ccagatgacc gggatccagc atcgacgcca gtttttccac cattgtcagt
    14641 cgcaggctaa gcggcgcatt taacatgccg ccgttcgtcc atgtctgaag ctgcacacgc
    14701 gaaagaagtt cctgcatcag tcgttcacga aactgctgct gatgggcttg tggaaggcgg
    14761 gcatcatcgc cctgcgccag atccactaaa aagcggggat aaaccgactc cagcacgcga
    14821 ccggggccgt ccagtaacgt cttggtcaat atcgttctgc cgtgaaaagt gtttgaatat
    14881 catcgcgtaa cagctgggcg tcggtgtaaa tccagccgtg agtcatcaca gtctgctgca
    14941 attgctgctg catcagcctg accaccgatt cattttgttg acgcagagcc aggctttcgc
    15001 gtaaacgcgt ctgtaattcc gtcaaacatg aagcgaactc agcgcaaaaa gtattcatgc
    15061 ctgccgtaac agattcatcg acctgctctg ccagaacttt agccatttgt tggcaataaa
    15121 gatcgacttc tgcgcttaaa gctcgttgca acacactgta atcaaccgtc tctgtcgggg
    15181 atttctcatt tccccgtccc cagtcgggct gattcaacca gcgcgaaaaa gtctcacgca
    15241 caacgcctaa acgcgtgctc tgctcgtccg ttgcatcctg gcgcgaaatg acttcactga
    15301 acagctggcg agtgttgacg tggggaacta ctccgtgaaa aacaggaaaa tgaaccccag
    15361 gacgaaaccc tgactcgctc aattccattt tgacttgttg ctcaatgggc cgaataacat
    15421 cggttaacac ccggcaaagg gtggattcaa gctcggcaaa acgcagcgta aagtcgcgac
    15481 tgatggtgtt ctgcgccgtc tgtaacagtg tctcacagca ggtacgaatc tcgcttaacg
    15541 gctccgaatc atcctgaaac aaggcggcta actgcgcatt cagcgcatct tgctgttgac
    15601 gcagaaagtg gttggcggag gtcagggcca gctcgatttc atgtttaatc tcgccgctca
    15661 cctgcgcctg attgagttgc aatagctgca aactttcttc gacctgatgg atattttgcc
    15721 gcaattgttc acaagcgacg tttaacccgt gcgcacgaaa atccaggtat tcccgcgcct
    15781 gctgcgcgta attcaacagt ttatgcgcag cagatcgcag agcatacaac gaggcgttag
    15841 cgtaagcggc atgaagcaac gcctgaattg gctgggcgaa cagcgaatct tcccacaact
    15901 gatcggcagc atgacgaata tgttcgaggt ccgccagatc ggcatgacgc cagcgcctgc
    15961 cgagcgcggc atgggcaaaa tcttccaccc agcgttgttg ctctggcgct ggtaacttac
    16021 cgttgttggc taactcatgg cgcgctcgat ttgccaagta gccccacatc gacgacaccg
    16081 gaaatatctg ctgtggcgta atacagcctt tcatcagcgt cccggaaatc agtgcccgca
    16141 cctggtcggc gtcgtcactg ttacgatcct gttgatcgaa cttattgacc agcacataca
    16201 gtggcaccga ttgccccacc gccaaaatcg cctcacggac ctcttcatcg gagatcgatt
    16261 tcagttgcgt ataatccagc accgccagta ccgccgaggc gcgtgccagc tgctggttaa
    16321 gcattttttg cagatgcggt tgcccggctt catttggccc gggggtatcc agtaacgtca
    16381 actgaccggg ataactctcc agccccgcca gatggacaaa ctccacttca atcacgggaa
    16441 tatgctcaat ggcggcgtaa gcagaaaaag gaaaatcgac gtccagcgcc ttcgccagtc
    16501 gcactaaatc attcaaactt ttcagacaat gaaaaatagg ctgggcaccc agataatatt
    16561 tttcgaaagc gacgccattt tcgatccgct gcataagcgc acgcatatct ttatctattt
    16621 ccagcacatc ggtcagatgc ttaatatcgc aatcacgcag gcgctgttgt aattgttgaa
    16681 ttaaacaatc gattggcgcg acatgtgaaa aatgcagtac cggttccttt tgcccgggcg
    16741 tatggcgaat aagcgtcggc agcgcagtca ttgggcgatt acgattaggc agaacctccg
    16801 taccaacaat ggcattaatg gtggttgatt tccctgcttt catagtaccg acaatcgcca
    16861 gcaccatttc cagtcgggaa attttactca actcattatt cagcatcgcg tgacgttcgg
    16921 cgatattagg ctgactccag ggtaaagcca gttgtggcgc gtcgtctccg ggtacagaga
    16981 gaggcatttt ttccagtaac tgcaactgtt ggcgagaaag ctgtaacagg cgttcagcct
    17041 cctgacttaa ctcatacagg gtctgtgtgt acatagaaaa ttcttcctta aagcaaattt
    17101 tgttactttt atttagccag attgtttttg agttctgttt gagttctgtt tttggctttt
    17161 ataattactg caagaaataa ttttatattt agtgtgttgt tttttatcag aataaataac
    17221 gtcttctgat gcgtttaaaa cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa
    17281 caagtagagc attaacatta tcttaaataa taaatagagg caaaaagatt attttctttt
    17341 tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact
    17401 cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt ggcgcttttg
    17461 ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa tgcaactccg
    17521 aatacactta cgaagataac ggcatgtaca tctgcccgga atgtgcctac gaatggaacg
    17581 acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac ggcaatctgc
    17641 tggctgacgg cgacagcgtt accatcatta aagatctgaa ggtgaaaggt agctcttcga
    17701 tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg
    17761 attgcaaaat cgacggtttt ggtccgatga aactgaaatc tgagtttgtg aaaaagaact
    17821 gattgtattg tgatcggtaa gccggataag gcgctcgcgc cgcatccggc aacgatgccg
    17881 actgcctgat gcgacgcttg cgcgtcttat caggcagcct ctcatctgta taaatttcga
    17941 actacactta attggcttct cttaactgag gtcaccatca tgccgttaag tccctacctc
    18001 tcttttgccg gtaactgtgc cgacgcaatt gcttattatc aacgtacgtt gggcgctgaa
    18061 ctgctctata aaatcagctt cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac
    18121 tgcccttccg gaatgcaatt tcccgatacc gccatcgccc acgccaatgt gcgcattgcc
    18181 ggaagcgaca tcatgatgag cgatgccatg ccgtcaggaa aagccagcta ctccggcttt
    18241 acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga caatcttgcc
    18301 gctaacggaa aaatcgaaat ggcctggcag gaaactttct aggcgcatgg ctttggcaaa
    18361 gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca accaacgcaa
    18421 taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct
    18481 gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga gccacttttt
    18541 tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa aaccttcaat
    18601 cagcatcagg cgctgcatgc ggttgctctg aacattcatc acggtgaaat ggtggctctg
    18661 cttgggccgt cgggttccgg caaatccacc cttttacgtc acttaagcgg tttgattacc
    18721 ggcgataaat ccgccggcag ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc
    18781 cgtctggcgc gcgatatccg caaaagccgc gccaacaccg gctacatctt ccaacaattc
    18841 aacctggtga accgcctgag cgtactggag aacgtgctga ttggcgcgct cggcagcacg
    18901 ccgttctggc gcacctgttt tagctggttt acccgcgagc agaaacaacg cgcgttacag
    18961 gcgctgaccc gcgttggcat ggtgcatttt gcccatcaac gcgtttccac cctctccggc
    19021 ggacagcagc agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg
    19081 gccgatgaac ccatcgcctc gctggacccg gaatccgccc gcatcgtgat ggacaccctg
    19141 cgcgacatca atcagaacga cggcatcacc gtggtcgtca cgctgcatca ggtggattac
    19201 gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtttt ctacgacggc
    19261 agcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat taatcgcatc
    19321 gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat gaacgctaag
    19381 ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt aagcccggca
    19441 cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga atcacagcaa
    19501 aacctgaaac cgcaatggac gccattctta caggatatgg agaagaagct gggcgtgaag
    19561 gtgaacgcct tctttgcccc agactacgca ggcattatcc agggaatgcg cttcaataaa
    19621 gtggatatcg cctggtacgg caacctgtcg gcaatggaag cggtggatcg cgccaacggc
    19681 caggtcttcg cccagacggt cgcggcggat ggatcgccag gttactggag cgtgttgatc
    19741 gtcaacaaag atagtccgat caacaacctg aacgatctgc tggcgaagcg gaaagatctc
    19801 accttcggca atggcgatcc taactccacc tctggcttcc tcgtccccgg ttactacgtc
    19861 tttgccaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc cgggcatgaa
    19921 accaacgcgc tggccgtcgc caacaagcag gtggatgttg ccaccaacaa caccgaaaac
    19981 ctcgacaagc tgaaaacctc cgcgccagag aagctgaaag aactgaaggt gatctggaag
    20041 tcgccgctga tcccaggcga tccgatcgtc tggcgcaaga atctctccga aaccaccaaa
    20101 gacaagatct acgacttctt tatgaactac ggaaaaacgc cggaagagaa agcggtgctg
    20161 gaacgcctgg gctgggcacc gtttcgcgcc tccagcgacc tgcaactggt gccgattcgc
    20221 cagctcgcac tgtttaaaga gattcagggc gtaaaaagca ataacggact gaatgagcag
    20281 gacaagctgg cgaaaaccac cgagattcag gcgcagctgg atgacctgga ccgcctgaac
    20341 aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc agtaatgtgc tcgatcggtc
    20401 ccctctccct tgagggagag ggttagggtg agggggaaat gcgcacgctg atgccctcac
    20461 cccggccctc tcccacaggg agagggagaa aacattaaac ggagccaaac atgcaaacca
    20521 tcaccatcgc cccacccaag cgcagctggt tctcgcttct gagctgggcc attgttctcg
    20581 ccgtgttggt cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg atcaaagacg
    20641 gcggcaacat ggcgaccttc gccgccgact tcttcccgcc cgatttcagc cagtggcagg
    20701 attacctcac cgaaatggcc gtcacgctgc aaatcgccgt ctggggcacc gcgctggcag
    20761 tggttctctc catccccttc ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt
    20821 accagcccgt tcgccgcctg atggacgcct gccgcgccat taacgaaatg gtcttcgcca
    20881 tgctgttcgt ggtcgccgtc ggtctcggac cgttcgctgg cgtgctggcg ctatttattc
    20941 acaccaccgg cgtgctctcg aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc
    21001 cggtggaagg cattcgcgcc accggcgcca acaagctgga agagatcctt tacggcgtgc
    21061 tgccacaggt aatgccgctg ctgatctcct actccctcta tcgcttcgaa tccaacgtcc
    21121 gctcggcgac cgtcgtcggc atggtcggtg caggcgggat cggcgtcacc ctgtgggaag
    21181 cgattcgcgg tttccagttc caacaaacct gcgccctgat ggtgcttatc atcgtcacgg
    21241 tcagcctgct ggatttcctc tctcaacggt tgcgtaagca ctttatctga taagtgaggc
    21301 attgatatct atgcacttgt ctacacatcc gaccagctac ccaacacgct atcaggagat
    21361 agccgcaaaa cttgagcagg agcttcgtca acactatcgc tgcggcgact atcttcccgc
    21421 cgagcagcaa ctggcggcgc gctttgaggt aaaccgccac accctgcgcc gcgccatcga
    21481 ccaactggtg gaaaaaggct gggtacagcg ccgtcagggc atcggcgtgc tggtgctgat
    21541 gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt agccagaacc tgctggacca
    21601 gggcagccat cccaccagcg aaaaactgct gtcggtattg cgccccgcgt ccggtcacgt
    21661 tgctgacgcg ctggggatta ccgagggtga gaacgttatc catttgcgca ccctgcgtcg
    21721 cgtcaacggc gtcgcgctct gtttaatcga ccactacttt gcggacctca ccctctggcc
    21781 gacactgcaa cgtttcgaca gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat
    21841 tgcgctgcgc cgcagccaga cgcggatcag cgcccgccgc gcccaggcca aagagtgcca
    21901 gcgccttgaa atcccgaata tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga
    21961 cggtgaaagc agcccggcgg agtactccgt cagcctgacg cgcgccgaca tgattgaatt
    22021 cactatggag cactgaatgc acgcagatac cgcgacccgc cagcactgga tgtccgtact
    22081 ggcgcacagc caaccggctg aactggcagc ccgcctgaac gcgctgaaca tcaccgccga
    22141 ctatgaagtg atccgcactg ccgaaactgg cctggtgcag attcaggcgc gaatgggcgg
    22201 taccggcgaa cgcttttttg ccggcgacgc cacgctgacc cgtgccgccg tacgcctgac
    22261 tgacggcacc ctcggctaca gctgggtgct ggggcgtgat aaacagcacg ccgaacgctg
    22321 cgcgctgatt gatgcgctga tgcagcaatc tcgccacttt caaaacttat cagaaaccct
    22381 tattgccccg ctggacgctg accgtatggc acgcattgcc gcacgccagg ccgaagtgaa
    22441 cgccagccgg gtcgacttct ttacgatggt tcgcggagac aacgcatgac cctggaaacc
    22501 gcttttatgc ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt aaaggccatg
    22561 agcgagccgg gcgtgattgt cgccctgtat cagctcaaac gcggctggca gccgctgaat
    22621 atcgccacca ccagcgtgct gctgacgctg gccgataacg acacgccggt gtggcttgcc
    22681 acgccattaa gtaacgatat cgtcaatcag agcctgcgtt ttcataccaa cgcgccgctg
    22741 gtcagccagc cggaacaggc gaccttcgcg gtgacggatg aggcgatttc cagcgaacag
    22801 ctcaacgccc tttccaccga caccgccgtt gcgccggaag cgggcgcgac gctgatttta
    22861 caggtcgcca gcctgagcgg cggacgcatg ttgcgtctca ccggcgcggg cattgccgaa
    22921 gaacgaatga tcgctccgca gctgccagag tgcattctgc acgaactcac cgagcgcccg
    22981 cacccgttcc cgctcggcat cgacctaatc ctgacctgcg gcgagcgcct gctggctatc
    23041 ccgcgaacca ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg
    23101 cgatcgacgc cgcccacgcc ctgcaagaga gccgacgccg gggcgatacc gatttgcccg
    23161 aactgagcgt cgcccagatt gaacagcagc ttaacctcgc ggtggatcgc gtgatgacag
    23221 aaggcggcat tgccgaccgc gaactggcgg cgctggcgct gaaacaggcc agcggcgata
    23281 acgtcgaagc gattttcctg ctacgcgcct accgcaccac gctgacgaaa ctggcggtaa
    23341 gcgagccgct cgacaccacg gagatgcgtc tcgaacggcg tatttcagcg gtttataaag
    23401 atattcccgg cggccagctg cttggcccaa cctacgacta cacccatcgc ctgctcgatt
    23461 tcactctgct ggcaaacggc gaagcgccga cgctgaccac cgccgacagc gaacaacagc
    23521 cgtcgccgca cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt gaagaggata
    23581 gcggagcaca gccggatgac atcacccgca cgccgccggt ttacccctgt tcacgttctt
    23641 cccgtttgca gcagttaatg cgcggcgacg aaggctattt gctggcgctg gcttactcca
    23701 cccagcgcgg ttacgggcgc aaccacccgt tcgcgggcga gatccgcagt ggttacatcg
    23761 acgtgtcgat tgtgccggaa gagctgggat ttgcggtaaa cgtcggcgaa ctgctgatga
    23821 ccgagtgtga aatggtcaac ggttttatcg acccgccgga tgagccgccg cacttcacgc
    23881 gcggctacgg gctggtattc ggcatgagcg agcgcaaagc gatggcaatg gcgctggttg
    23941 accgtgcgtt gcaagccccg gaatacggcg agcacgcgac aggcccggcg caggatgaag
    24001 agttcgtgct ggcgcatgcc gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac
    24061 tcccgcacta cgtcgatttc caggccgaac tggagctact caaacgtctg caacaggagc
    24121 agaaccatgg ctaatctgag cggctacaac tttgcctacc tcgacgagca gaccaaacgc
    24181 atgatccgcc gcgccatctt aaaagcggtg gcgatccccg gttatcaggt accgtttggc
    24241 gggcgcgaga tgccgatgcc gtacggctgg ggaactggtg gtatccagct caccgccagc
    24301 gtgattggcg aaagcgacgt gttgaaggtg attgaccagg gtgcggatga caccaccaac
    24361 gccgtgtcga ttcgcaactt ctttaagcgc gtgaccgggg taaacaccac tgaatgtacg
    24421 gacgatgcga cgcttatcca gacgcgtcac cgcatccccg aaacgccgct gaccgaagat
    24481 cagatcatta tcttccaggt gccaatcccg gaaccgctgc gctttatcga gccgcgcgaa
    24541 acggagaccc gcactatgca cgcgctggag gagtacggcg tgatgcaggt gaaactgtat
    24601 gaagatatcg cccgcttcgg tcatatcgcc accacctatg cttatccggt gaaggtgaac
    24661 gggcgctacg tgatggaccc gtcgccgatc ccgaaattcg ataacccgaa aatggacatg
    24721 atgcccgccc tgcagctgtt cggcgcaggg cgcgagaagc gcatctatgc ggtgccgccg
    24781 tttacccgcg tggaaagtct cgatttcgac gatcacccgt tcaccgttca gcagtgggat
    24841 gagccatgcg ccatctgcgg atcgacccac agctatcttg atgaagtggt gctggatgac
    24901 gccggaaacc gcatgtttgt ctgctccgat accgattatt gccgccaaca gagcgaggcg
    24961 aaaagccaat gaatcaaccg ttactttcgg tcaataacct gacccacctt tacgcgccgg
    25021 gcaaaggctt tagcgatgtc tcttttgatt tatggccggg ggaagtgctg ggcattgtcg
    25081 gggaatccgg ctccgggaag accacgctgc tgaagtcgat ctccgcgcgt ctgacgccgc
    25141 agcaggggga gattcactat gaaaaccgtt ccctctatgc aatgagcgag gccgaccgcc
    25201 gtcgcctgct gcgcaccgaa tggggcgtgg tgcatcagca tccgctcgac ggcctgcgcc
    25261 gccaggtgtc ggcaggcggc aatatcggcg agcggctgat ggcgaccggg gcgcgacatt
    25321 acggcgatat tcgcgctacc gcgcagaagt ggctggaaga ggtggagatt cccgccaacc
    25381 gtatcgacga cctgccgacc accttttccg gcggtatgca gcagcgtttg cagattgccc
    25441 gcaacctggt gacgcatccg aagctggtgt ttatggatga accgaccggc gggctggatg
    25501 tgtcggtgca ggcccgcctg ctcgacctgc tgcgcggact ggtggtggag ctgaacctcg
    25561 cggtagtgat tgtcacccac gatttaggcg tcgcccgcct gctggcggac cgtttgctgg
    25621 tgatgaagca ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc
    25681 atcatccgta tacacagctg ctggtgtcat cggttttgca gaattgagcc ggtgccggat
    25741 gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga taagacgcgt
    25801 cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag
    25861 taaaaccttc atcctgcacc agcaaaacgg cgtgcgcctg cccgtcctca accgcgcctc
    25921 gctcatcgtc aacgcgggcg aatgcgtggt gctccacggc cattccggca gcggaaaatc
    25981 aaccctgcta cgctcgctgt acgccaacta tctgcccgac gaagggcaaa tccagatcaa
    26041 acacggtgac gaatgggtcg atctggtcac cgcgccagcg cgcaaagtgg tggagatccg
    26101 caaaaccacc gttggctggg tgagccagtt tctgcgcgtc atcccgcgta tttcggcgct
    26161 ggaagtggtg atgcagccgc tactcgatac cggcgtaccg cgtgaagcct gcgccgctaa
    26221 agccgcgcgt ctgctcaccc gcctgaacgt gccggaacgc ctgtggcatc tggcgccatc
    26281 gaccttctcc ggcggcgaac agcagcgcgt caacatcgcc cgcggcttta tcgtcgacta
    26341 ccccattctg ctgctcgacg aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt
    26401 agtcgagctg attcacgaag ccaaagcccg tggcgcggcc atcgtcggca tcttccacga
    26461 cgaagccgta cgtaatgacg ttgccgaccg cctgcaccca atggaagcct cttcatgatt
    26521 atcaataacg tcaaattggt gctggaaaac gaagtggtac acggttcact ggaggtgcag
    26581 gacggcgaaa tccgcgcctt tgccgaaagc cagagccgcc tgccggaggc gatggacggc
    26641 gaaggcggct ggctgctgcc ggggctgatt gagctgcata ccgataatct ggataaattc
    26701 ttcaccccgc gcccgaaagt tgactggcct gcccactcgg cgatgagcag ccacgacgcg
    26761 ctgatggtgg cgagcggcat caccaccgta ctggatgccg tggcaattgg cgacgtgcgc
    26821 gacggcggcg atcggctggt gaatctggag aagatgatca acgccatcga agagacgcag
    26881 aaacgcggcg tcaaccgcgc cgagcaccgt ctgcatctgc gctgcgaact gccgcatcac
    26941 accacgctgc cgctgtttga aaaactggtg cagcgcgagc cggtgacgct ggtgtcgctg
    27001 atggaccact cgccgggcca gcgccagttc gccaatcgcg agaagtatcg cgaatattat
    27061 cagggcaaat actccctcac tgatgcgcag atgcagcagt acgaagaaga gcaactggcg
    27121 ctcgccgcac gctggtcgca gccgaatcgc gaatccatcg ccgccctgtg ccgcgcgcga
    27181 aaaattgcgc ttgccagcca cgatgacgcc acccacgccc acgttgctga atctcaccag
    27241 cttggcagcg tgatcgccga atttcccacc acgttcgaag cggcggaagc ctcgcgcaag
    27301 catggcatga acgtgctgat gggcgcgccg aatattgtgc gcggcggctc gcactccggc
    27361 aacgtggcgg ccagtgaact ggcgcagctt ggcctgctgg atatcctctc ttccgactac
    27421 taccccgcca gcctgctcga tgcggcattt cgcgtcgccg atgacgagag caaccgcttt
    27481 acgctgccgc aggcggtgaa gctggtgact aaaaatccag cgcaggcgct taatctccag
    27541 gatcgcgggg tgattggcga gggcaaacgc gcggacttgg tgctggcgca tcgcaagggc
    27601 aatcacattc atatcgacca cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa
    27661 aactgatttg gttaatgggg ccgtccggct ccgggaaaga tagcctgctg gcggaactcc
    27721 gcctgcggga acaaactcag ctactggtgg cgcatcgcta catcacgcgc gacgccagcg
    27781 ccggaagcga aaaccatatc gctctgagcg agcaggagtt ttttacccgc gcggggcaga
    27841 acctactggc cttaagctgg cacgctaacg gtctgtatta tggcgtcggc gtcgagatcg
    27901 acctctggct gcacgccgga ttcgacgtgc tggtcaacgg ctcacgcgcc catctgccgc
    27961 aggcgcgggc gcgctatcaa tcggcgctgc tgcccgtctg tttacaggtt tcgccggaga
    28021 tcctccgcca gcgcctggaa aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccgcc
    28081 tggcgcgcgc cgcccgctat actccacagg attgccatac gctcaacaat gacggcagcc
    28141 tgcgccagtc ggtcgacacg ctgctgacgc tgattcatca gaaggagaaa caccatgcct
    28201 gcttgtgagc ttcgcccggc cacgcagtat gataccgacg cggtttacgc gctgatttgt
    28261 gagctaaaac aggcggagtt tgaccaccac gcatttcgcg tgggctttag cgccaatctg
    28321 cgcgacccga acatgcgcta tcatctggcg ctgctggatg gcgaggttgt cggcatgatc
    28381 ggcctgcatt tgcagtttca tctgcatcat gtcaactgga tcggcgaaat tcaggaactg
    28441 gtagtgatgc cgcaggcgcg cggcctgaac gtcggtagca agttgctggc gtgggcagaa
    28501 gaagaagccc gccaggccgg ggccgaaatg accgagcttt cgaccaacgt gaagcgccac
    28561 gacgcgcacc gtttctatct gcgagaaggc tacgagcaga gtcact
//
LOCUS       DAJTVE010000042        28511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000042 DAJTVE010000000
VERSION     DAJTVE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1046)
                     /locus_tag="OO439_001765"
     CDS             complement(<1..1046)
                     /locus_tag="OO439_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6316150.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OO439_001766"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OO439_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OO439_001767"
     CDS             complement(2433..3281)
                     /locus_tag="OO439_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6316151.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OO439_001768"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OO439_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6316152.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OO439_001769"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OO439_001769"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6316153.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO439_001770"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO439_001770"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6316154.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OO439_001771"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OO439_001771"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6316155.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OO439_001772"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OO439_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6316156.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OO439_001773"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OO439_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6316157.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OO439_001774"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OO439_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6316158.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OO439_001775"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OO439_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6316159.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO439_001776"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO439_001776"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6316160.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OO439_001777"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OO439_001777"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6316161.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OO439_001778"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OO439_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OO439_001779"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OO439_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OO439_001780"
     CDS             22669..23652
                     /locus_tag="OO439_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6316162.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OO439_001781"
     CDS             23675..25186
                     /locus_tag="OO439_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6316163.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OO439_001782"
     CDS             25211..26209
                     /locus_tag="OO439_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6316164.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OO439_001783"
     CDS             26242..27336
                     /locus_tag="OO439_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6316165.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OO439_001784"
     CDS             27348..28220
                     /locus_tag="OO439_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6316166.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28511)
                     /locus_tag="OO439_001785"
     CDS             complement(28268..>28511)
                     /locus_tag="OO439_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6316167.1"
                     /translation="AVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTG
                     DAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6757 a         7050 c         7753 g         6951 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggttt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc a
//
LOCUS       DAJTVE010000043        28440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000043 DAJTVE010000000
VERSION     DAJTVE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(175..381)
                     /locus_tag="OO439_001786"
     CDS             complement(175..381)
                     /locus_tag="OO439_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6316168.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            694..783
                     /gene="kdpF"
                     /locus_tag="OO439_001787"
     CDS             694..783
                     /gene="kdpF"
                     /locus_tag="OO439_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6316169.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            783..2456
                     /gene="kdpA"
                     /locus_tag="OO439_001788"
     CDS             783..2456
                     /gene="kdpA"
                     /locus_tag="OO439_001788"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6316170.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2479..4527
                     /gene="kdpB"
                     /locus_tag="OO439_001789"
     CDS             2479..4527
                     /gene="kdpB"
                     /locus_tag="OO439_001789"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6316171.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4536..5108
                     /gene="kdpC"
                     /locus_tag="OO439_001790"
     CDS             4536..5108
                     /gene="kdpC"
                     /locus_tag="OO439_001790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6316172.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5101..7785
                     /gene="kdpD"
                     /locus_tag="OO439_001791"
     CDS             5101..7785
                     /gene="kdpD"
                     /locus_tag="OO439_001791"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6316173.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7782..8459
                     /gene="kdpE"
                     /locus_tag="OO439_001792"
     CDS             7782..8459
                     /gene="kdpE"
                     /locus_tag="OO439_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6316174.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8734..8838
                     /locus_tag="OO439_001793"
     CDS             8734..8838
                     /locus_tag="OO439_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6316175.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9149..11347
                     /gene="speF"
                     /locus_tag="OO439_001794"
     CDS             9149..11347
                     /gene="speF"
                     /locus_tag="OO439_001794"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6316176.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11344..12663
                     /gene="potE"
                     /locus_tag="OO439_001795"
     CDS             11344..12663
                     /gene="potE"
                     /locus_tag="OO439_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6316177.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12719..14359)
                     /gene="pgm"
                     /locus_tag="OO439_001796"
     CDS             complement(12719..14359)
                     /gene="pgm"
                     /locus_tag="OO439_001796"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6316178.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14385..14930)
                     /gene="seqA"
                     /locus_tag="OO439_001797"
     CDS             complement(14385..14930)
                     /gene="seqA"
                     /locus_tag="OO439_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6316179.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15115..15879
                     /gene="ybfF"
                     /locus_tag="OO439_001798"
     CDS             15115..15879
                     /gene="ybfF"
                     /locus_tag="OO439_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6316180.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16019..16312
                     /gene="ybfE"
                     /locus_tag="OO439_001799"
     CDS             16019..16312
                     /gene="ybfE"
                     /locus_tag="OO439_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6316181.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16452..16982
                     /gene="fldA"
                     /locus_tag="OO439_001800"
     CDS             16452..16982
                     /gene="fldA"
                     /locus_tag="OO439_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6316182.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17192..17278
                     /gene="uof"
                     /locus_tag="OO439_001801"
     CDS             17192..17278
                     /gene="uof"
                     /locus_tag="OO439_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6316183.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17271..17717
                     /gene="fur"
                     /locus_tag="OO439_001802"
     CDS             17271..17717
                     /gene="fur"
                     /locus_tag="OO439_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6316184.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17802..18128)
                     /locus_tag="OO439_001803"
     CDS             complement(17802..18128)
                     /locus_tag="OO439_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316185.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18178..19584)
                     /gene="chiP"
                     /locus_tag="OO439_001804"
                     /pseudo
     CDS             complement(18178..19584)
                     /gene="chiP"
                     /locus_tag="OO439_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20162..21826)
                     /gene="glnS"
                     /locus_tag="OO439_001805"
     CDS             complement(20162..21826)
                     /gene="glnS"
                     /locus_tag="OO439_001805"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6316186.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22085..24031)
                     /gene="nagE"
                     /locus_tag="OO439_001806"
     CDS             complement(22085..24031)
                     /gene="nagE"
                     /locus_tag="OO439_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6316187.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24364..25164
                     /gene="nagB"
                     /locus_tag="OO439_001807"
     CDS             24364..25164
                     /gene="nagB"
                     /locus_tag="OO439_001807"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6316188.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25224..26372
                     /gene="nagA"
                     /locus_tag="OO439_001808"
     CDS             25224..26372
                     /gene="nagA"
                     /locus_tag="OO439_001808"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6316189.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26381..27601
                     /gene="nagC"
                     /locus_tag="OO439_001809"
                     /gene_synonym="nagR"
     CDS             26381..27601
                     /gene="nagC"
                     /locus_tag="OO439_001809"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6316190.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27649..28401
                     /gene="nagD"
                     /locus_tag="OO439_001810"
     CDS             27649..28401
                     /gene="nagD"
                     /locus_tag="OO439_001810"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6316191.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6669 a         7238 c         7466 g         7067 t
ORIGIN      
        1 ccttaagtga gatatccgtc agatagaaac aattaggaaa atatttatat atatttcatg
       61 aatatatgtt tcgattggta taacggtgtc taaatattcc tctgaacatc ttcctgatac
      121 aaaaaaccgc cagcgtttcc gctggcggtt ttcaatgtat tcagaactca gcaatcagta
      181 aaaatcaccg gttgcctttt cggcctgatc catccacacc ggtttatcgc tggttttcga
      241 ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg
      301 cagcgccaga acgcccgctg caaccgcata tgtacggcgt aaaaagataa gccatgcagg
      361 atattctctg tagagttcca tacttttctc ccccttttgt atctacccgg tgaatagcac
      421 cggaaaaatg aatttgttta tctgatgaaa atagtaccgc cttttgtgta attttactac
      481 tcatccgacc acttattttt gcttattgat ggtttattta cattcactct gtaattaagt
      541 tacacaaaag ttaaattaat actaaacatt agttaaatca tggcttttgc catttttata
      601 ctttttttac accccgcccg cagatttttg cgaaatcttt gcagccagaa ttctactctt
      661 ccggtatcac ttttaggcca ctggaggtgc actgtgagtg caggcgtgat agccggcgtg
      721 ttgctggtgt ttttattact gggttatttg gtttatgccc tgatcaatgc ggaggcgttc
      781 tgatggctgc gcaagggttc ttactgattg ccacgttttt actggtatta atggtgctgg
      841 cgcgtccttt aggcagcggg ctggcgcggc tgattaatga cattcctctt ccaggtacag
      901 cgggcgttga acgtatactc tttcgtttac ctggcgtctc tgaccatgag atgaactgga
      961 agcaatatct ttgtgccatt ctcggcctga acatgctggg gctggcggtg ctgtttttta
     1021 tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccgggg ctgtcgtggg
     1081 atctggcgct gaataccgcc gtcagctttg tcacgaatac caactggcaa tcttatagcg
     1141 gtgaaaccac gttgagctat ttcagccaga tggcaggctt aacggtacaa aactttcttt
     1201 ctgccgccag cgggattgcg gtgatttttg ccttcatccg tgcgtttact cgccagagca
     1261 tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg gtgctagtcc
     1321 ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcgctgcaa aactttctgc
     1381 cctatcaggc agtgaatacc gttgaaggag cgcaacagct gttacccatg ggaccagtag
     1441 cttctcagga agcgatcaag atgctcggta ctaacggcgg tggcttcttt aatgccaact
     1501 cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct
     1561 tcttgatccc aacagcgctg tgctttgcct ttggtgaagt gacgggcgat cgccgccagg
     1621 ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc gtggttatgt
     1681 gggcagaagt tcagggtaat cctcatctgc tggcgctcgg cgcagacagc agcatcaata
     1741 tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta
     1801 cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct ctcggcggca
     1861 tggtgccgat gtggctaatg caaattggtg aagtggtgtt cggcggtgtc ggttctggtc
     1921 tttacggcat gatgctgttt gtcctgctgg cggtgtttat tgccgggctg atgattggtc
     1981 gtacaccgga atatctgggt aaaaaaatcg acgtacgcga gatgaaactg actgcactgg
     2041 cgattctggt tactccaaca ctggtactga tgggcgcggc gttggcgatg atgaccgacg
     2101 ccggacgtag cgccatgctt aaccctggcc cgcatggttt tagcgaagtg ctgtacgccg
     2161 tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc aactctccgt
     2221 tctggaactg tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg
     2281 tgatggcaat tgccggttcg ctggtgagta aaaagagcca acccgccagc tccggcacgc
     2341 tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg ctggttggcg
     2401 cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc tcctgatgat
     2461 attgagcgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc aacacttgtc
     2521 gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg
     2581 gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat cgcgatggca
     2641 agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc
     2701 accgtactgt tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc
     2761 aacagtctta aaggggtgaa aaaaactgcc tttgcccgca agctgcgtga gccgaaatat
     2821 ggcgctgcgg cggacaaagt tcctgccgat caactgcgta aaggtgacat cgtgctggta
     2881 gaagctggcg atattatccc ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat
     2941 gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg aatccggcgg cgattttgcc
     3001 tccgtcaccg gcggcacgcg tattctttct gactggctgg tgattgagtg tagcgttaac
     3061 cccggtgaga catttctgga tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa
     3121 acaccgaacg agattgccct gactatcctg ctgattgctc tgaccatcgt ctttttactg
     3181 gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgccgtgag tgtaacggta
     3241 ctggtggcgc tgctggtctg tctgatccca accactattg gcggcctgtt gtcagcgatc
     3301 ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag cggacgtgca
     3361 gttgaagcgg caggtgacgt tgacgttctg ctactggata aaaccggcac catcacactc
     3421 ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg tggatgaaaa aacgctggct
     3481 gacgccgcac aactggcttc gctggctgat gaaacgccgg aaggtcgcag tattgtgatc
     3541 ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt
     3601 gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg catgatccgt
     3661 aaaggttctg tcgatgccat tcgccgccat attgaggcta acggcggtca cttccctgcc
     3721 gatgttgatc aaaaagtcga tcaggttgcg cgtcagggag ccacgccgct ggtggtggtg
     3781 gaaggttctc gtgtgctggg cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa
     3841 gagcgcttcg cccagctgcg caaaatgggc attaaaacgg tgatgattac cggcgataac
     3901 cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg atgattttct cgccgaagca
     3961 acaccggagg ccaagctggc attgatccgt cagtatcagg cggaaggtcg tttggtagcg
     4021 atgaccggcg acggcaccaa cgatgctccg gcgctggcgc aggcagatgt cgcggtggcg
     4081 atgaactccg gcacccaggc ggcgaaagag gcgggcaata tggtggatct cgactctaac
     4141 ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga tgctgatgac ccgtggctcg
     4201 ctgaccacct tcagcattgc caacgatgtg gcgaaatact tcgccattat tccggcggcg
     4261 ttcgcggcaa catatccgca gttaaatgcg ctgaacatca tgcgcctgca ttcgcccgac
     4321 tccgccattc tcagtgcggt cattttcaac gccttgatta tcgtcttttt gattcctctg
     4381 gcgttaaaag gcgtgagtta taaaccgctt accgcttctg ccatgttgcg ccgtaactta
     4441 tggatttacg gtctgggtgg gctgctggtg ccgtttattg gtatcaaagt cattgattta
     4501 ctgctgaccg tttgcggtct ggtgtgaggt ttaccatgag tggattacgt ccggcattat
     4561 cgacatttct ctttctgtta ttgattactg gcggcgttta cccgctgctg accaccgcac
     4621 tggggcaatg gtggtttccc tggcaggcca acggttcgtt gatccgtgaa ggtgatacgg
     4681 tgcgtggctc ggcattaatc gggcagaatt ttaccggcaa cggctatttt catggtcgcc
     4741 cgtcagcaac ggctgaaatg ccctataatc cacaggcttc tggcgggagc aatctggcgg
     4801 tcagtaaccc tgagctggat aaactaatag ccgcacgcgt tgctgcatta cgggccgcta
     4861 acccggatgc cagcgcgagc attccggttg aactggtgac ggcatcggca agcgggctgg
     4921 acaataatat caccccgcaa gcggcggcct ggcagatccc acgcatcgcg aaagcgcgta
     4981 atctcagcgt tgaacagctc acgcagctga tcgcaaaata cagccaacaa ccgctggtga
     5041 aatatatcgg ccagccggtc gtcaacattg tcgaactcaa tctggcgctg gataagcttg
     5101 atgaataacg aacccttacg tcccgacccc gatcgtctgc tggaacaaac tgccgcgccg
     5161 catcggggga agctgaaagt tttcttcggt gcctgtgcag gcgtcgggaa gacctgggcg
     5221 atgctggcag aagcccagcg actgcgggcg caagggctgg atattgtggt tggcgtggta
     5281 gaaacccacg ggcgaaaaga taccgccgca atgctggaag ggctggctgt tctgccgcca
     5341 aaacgcctgg cgtaccgtgg gcggcacatc tgcgagtttg acctcgatgc cgccctcgcc
     5401 cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca gtaatgcgcc aggttctcgt
     5461 caccctaaac gctggcagga catcgaagag ctgctggaag caggcattga tgttttcact
     5521 accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg tcagcggcgt caccggaatt
     5581 caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg ccgacgacgt ggttctggtg
     5641 gacttgcccc cggacgatct gcgccagcgg ctgaaagaag gcaaagtcta tattgccggg
     5701 caggcggagc gcgccattga acattttttc cgcaaaggca atctgatcgc cctgcgcgaa
     5761 ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa tgcgcgcctg gcgggggcat
     5821 cctagcgaag agaaagtctg gcacacgcgc gacgcgatcc ttttatgcat cggccataac
     5881 accggtagtg agaagctggt ccgcgcggcg gcgcggctgg cgtcacggct gggtagcgtc
     5941 tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct taccggagaa aaaacgtcgt
     6001 gcaattctca gcgccttacg tctggcgcag gaactgggcg cggagacggc aacactttct
     6061 gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg aacataatct cggcaagatt
     6121 attctcggtc gcccggcctc gcgccgctgg tggcgtcggg aaacgtttgc tgaccgactg
     6181 gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc
     6241 acgattaaca acgcgccgga tagccgctct tttaaagaca agtggcgggt acagattcag
     6301 ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct taattgccat gcagtggctg
     6361 atggcgtttg atgccgccaa cctggtgatg ctgtatctgc ttggcgtggt ggtggtggcg
     6421 ctattttatg gacgctggcc ttcggtggtt gccaccgtca ttaatgtggt gagtttcgat
     6481 ctctttttta tcgccccacg cggcacgctc gccgtctctg atgtgcaata tctgctgacc
     6541 ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc ttactgctgg cgtgcgttat
     6601 caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc acttatatga aatgtcgaaa
     6661 gctctggcgg tgggccgcag tccgcaggac atcgccgcca ccagcgaaca atttattgcc
     6721 tccacgtttc atgcccgcag tcaggtgttg ttgcccgatg acaacggtaa attgcagccg
     6781 ttaacacatc cgcaaggaat gacgccatgg gacgatgcca tcgcgcagtg gagttttgat
     6841 aaaggcctgc ctgcgggcgc gggcaccgac acgttacccg gtgtaccgta ccagattttg
     6901 ccgctaaaaa gcggcgagaa aacctacggg ctggtggtgg tggagccggg gaatctgcgc
     6961 cagttgatga tcccggaaca gcagcgcctg ctggagacgt ttacgctgtt agtcgccaat
     7021 gcccttgagc ggctgacgct aaccgccagc gaagaacagg cgcggatggc aagcgaacgt
     7081 gaacagatcc gcaacgccct gctggcagcg ctttcgcatg atttacgcac gccgcttacg
     7141 gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg caagcgaagg atcaccccac
     7201 gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca ctacccgact ggtgaataat
     7261 ctactggata tggcgcgaat tcagtccggc ggctttaatt tgaagaaaga gtggttaacg
     7321 ctggaagaag tagtcggcag cgcgctgcaa atgctggaac cgggtttatc gtcgcccatc
     7381 aatctttctc tgccagaacc gctgacctta atccacgttg acgggccact ctttgaacgg
     7441 gtgctgatta atctgctgga gaacgcggtg aaatatgcgg gtgcgcaggc cgaaattggt
     7501 atcgatgccc actttgaggg cgaaaatcta caactggatg tctgggataa cggccccggt
     7561 cttccgccag gccaggagca gacgatattt gataagtttg ctcgcgggaa taaagagtcg
     7621 gcggtgccgg gtgtcgggct tgggttggca atttgtcggg cgattgtgga tgtacacggc
     7681 ggcaccatta ccgcgttcaa ccgaccggaa ggtggtgcct gttttcgtgt tacacttccc
     7741 cagcaaaccg ctcctgaact tgaagacttt catgaggata tgtgacaaac gttctgattg
     7801 ttgaagatga acaggctatt cgtcgctttc tgcgcacggc gctggagggc gacgggatgc
     7861 gcgtctttga ggccgaaact ctgcaacgcg gcttgctgga agcggcaacc cgtaagccag
     7921 atttgattat tctcgatctc ggcctgcccg atggtgatgg gattgagttt atccgcgacc
     7981 tgcgccagtg gagcccggtg ccggtgattg tgctttccgc acgcagcgaa gagagcgaca
     8041 agatcgccgc gctggatgcc ggagcggatg attatctgag taagccgttt ggcattggcg
     8101 aattgcaggc ccgtctgcgc gtcgctttac gccgccactc cgccaccgcc gcgcccgatc
     8161 cgctggtgaa attttccgat gttaccgtcg atttagccgc ccgcgtgatt caccggggtg
     8221 aggaagaggt gcatctcaca ccaattgagt tccgcctgct ggcggtgctg ctcaacaatg
     8281 ccggaaaagt actcacccag cgccagctcc ttaaccaggt gtgggggcca aacgcggtcg
     8341 aacacagtca ctatttgcgt atttatatgg gacatctgcg acaaaaactg gaacaggatc
     8401 cggcccggcc tcgccacttc attactgaaa ccggtattgg atatcggttt atgctttgaa
     8461 ttgttattca ataaattcgc ttaaatagtg attaatttat tattaaacct gcgccgaata
     8521 ttattatttg gcaggtttaa tttcttaacg caaatataaa acacgaaatt acaacattta
     8581 agtaacaaaa cagcatctaa tatggcgata ttcataatta atgatttatt atctggggta
     8641 tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata gaataactct
     8701 accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg cttaatgcct
     8761 catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt tgactttcat
     8821 ttctttaatg cccgctagtc ttctgactaa agggcacccc aacgtacagg tctccctgac
     8881 tttaagcatt acaggttaat acctgtattc ctcggtgctc atatactgct aaccctttta
     8941 aactctaaat aattcgagtc gcagcacttg caacttgagg tatgacgagt atagccagtt
     9001 accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta tttatcagcg
     9061 gtggagcttt gctttttttc cggcgtgatc gatttctcct ttgagaaatt gaggacctgc
     9121 tattacctga aataaagaga tgaataaaat gtcagaatta aaaattgccg ttagccgttc
     9181 ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat attgacgaaa gtaattatat
     9241 tgacgttgcc gccattattt tatcagtcaa tgatgttgaa cgtggaaaac tcgatgaaat
     9301 agacgctact ggctatggca ttcctgtttt tattgcaacg gaaaatgaag aacgtgtacc
     9361 agcagaatat ctgccacgta tttctggtgt cttcgagcat tgcgaatcac ggaaagagtt
     9421 ttatggacgt cagttagaaa ccgctgctag ccattatgaa acgcaactgc gcccgccctt
     9481 cttccgcgca ctggtcgatt atgtcaatca gggcaatagc gcttttgact gcccgggtca
     9541 tcagggaggt gagtttttcc gtcgtcatcc ggcagggaat cagttcgtgg agtacttcgg
     9601 agaaacgctg ttccgttccg acttatgcaa cgcagacgta gcaatgggcg atctgctgat
     9661 tcacgaaggc gcgccatgca ttgcacagca acatgcggca aaagtgttta atgccgataa
     9721 aacatacttc gttctaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct
     9781 aacaccgggt gatctggtgc tgtttgatcg caataaccac aaatctaacc accacggagc
     9841 gttgctacag gctggtgcaa caccggttta tctggaaacg gcacgtaacc cgtatggctt
     9901 tatcggtggc attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga
     9961 agtcgcaccg cagcgggcaa aagaggctcg tcctttccgc ctcgctgtga ttcagttagg
    10021 cacctacgac ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg
    10081 tgactacatt ctgtttgact ccgcatgggt tggctatgag caatttatcc cgatgatggc
    10141 tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca
    10201 atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag
    10261 ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat
    10321 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
    10381 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc
    10441 ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga
    10501 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca
    10561 gtttgtacca ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt
    10621 cgatccttgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga
    10681 agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc
    10741 ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa
    10801 acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc
    10861 attagcagaa gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
    10921 cctgcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg tgaaacaact
    10981 gcaaaaagag atgttccgca aagcgcactt cccacacgtc agcatgaatc cgcaagaagc
    11041 caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggtcg
    11101 tatcgctgcc gaaggggcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga
    11161 aatctggggt ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct
    11221 gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
    11281 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa
    11341 attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg
    11401 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca
    11461 atttcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
    11521 gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct
    11581 ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcact gctgattgct
    11641 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
    11701 tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac
    11761 tttggtggtg cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg ggtcattatt
    11821 ccggtggtag gcctgtgcat tatcggctgg ttctggttta gcccgacgct ttacgttgat
    11881 tcctggaatc cgcatcatgc accgttcttc agtgcagtag gttcttccat cgctatgacg
    11941 ctgtgggctt ttctcggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg
    12001 gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc
    12061 gtctccacca acgtgattgc agggattgtg cctaatatgg agctggcaaa ttcaacggca
    12121 ccatttggcc tggccttcgc acagatgttc acaccagaag tgggtaaagt cattatggcg
    12181 ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg
    12241 tttaagtctt catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg
    12301 gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg
    12361 acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc
    12421 aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat
    12481 gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtatagc
    12541 ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat cgtgaccttc
    12601 cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa taaacacggt
    12661 taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc tttttttatt aaatgtgttt
    12721 acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat
    12781 gttcttcacc gaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
    12841 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccgatagaa gcaccattgc
    12901 ccggtgccgc cgtcaggcgc gcggtgatcg ggtcgcctgc cagggtgctg gcgctcacca
    12961 tttccggaga cagcttagac agcgccgctt tttgtgcgga agtcgcagct gcctgcaaac
    13021 ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct
    13081 taccggtgac agcggtgatt tccgccgcca gcagacacat gatgatgccg tctttgtcgg
    13141 tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa
    13201 agccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc gggacttcta
    13261 ccagcttgcg gcccaagtca ttgaccacac ggtcgatcat cgcagaagaa accagcgttt
    13321 taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtag ttgatcgcca
    13381 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
    13441 aatccgggtc gttagcaaac gccagatcga acttatcacg caatgccagc aggcccgcca
    13501 tcgcacactc ggaggagcag tccatacgga tcgcgccgtc tttatcgagg tgcataaagc
    13561 ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa
    13621 ttcgcttcca gtattcgata ccggaaccac ccagcggatc aacgcccagc gtcaggcccg
    13681 ttttctgaat cgccgccata tcaacgatat cggccagacc ttccacaaac tgctgcacca
    13741 gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
    13801 ctttcaggcc atcggccatc agtgcgttgg cacgatcttc caccacttta gtgacgttgg
    13861 tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat
    13921 gggagggtgt aatcacgata ccgtctgcca gcgggccacc ttttttatta tgaaccagga
    13981 tggcattgga aacggcaggc gtcggagtga agccattgtt ttcctgcaca atgacatcaa
    14041 cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag
    14101 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
    14161 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
    14221 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
    14281 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
    14341 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
    14401 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
    14461 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgcctgg cacatgtttc
    14521 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt
    14581 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
    14641 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtcagaa
    14701 agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg
    14761 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
    14821 gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
    14881 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
    14941 tacagtgtct tagctgaata caagtcgcca agagtatatg aataaatccc ggcagcggat
    15001 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
    15061 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
    15121 ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
    15181 catggtctgt ttggtagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
    15241 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga tccggtaatg
    15301 aattacccgg cgatggcgca ggatcttgtt gatactctgg atgcacagca gatcgacaaa
    15361 gcaacattta tcggtcactc catgggcggt aaagcggtaa tggcacttac ggcactggcc
    15421 cgcgatcgca tcgataaact ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc
    15481 cgtcatgatg agattttcgc ggctatcaac gcggtcagtg aatcggacgc acaaactcgc
    15541 cagcaagcag cagcaataat gcaccagcat cttaatgaag aaggggtgat tcagtttctg
    15601 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat
    15661 ccgcatattg tgggttggga gaaaatcccg gcatgggatc accctgccct gtttatccct
    15721 ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
    15781 caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
    15841 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
    15901 cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg
    15961 cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat
    16021 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
    16081 acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca
    16141 gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc
    16201 tgtcgaggcg ctgaacgagc tggcggagtc gcgcaatatg agccgtagcg aactgatcga
    16261 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
    16321 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
    16381 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
    16441 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
    16501 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
    16561 caaaaagcag caaagaagat ctggaagctt atgacattct gctgctgggc atcccaacct
    16621 ggtattatgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
    16681 atttcaacgg caaactggtt gcgctgtttg gttgtggtga ccaggaagat tacgccgaat
    16741 atttctgcga cgcattgggc accatccgcg acatcattga accgcgcggt gcaacgatcg
    16801 ttggtcactg gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg gctgatgacg
    16861 accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc gctgaacgtg
    16921 tagaaaaatg ggttaaacag atttctgaag agttgcatct cgacgaaatt ctcaatgcct
    16981 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
    17041 attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt gttcaagtgg
    17101 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
    17161 gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
    17221 tgtcacttct tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
    17281 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctag
    17341 aagttcttca ggaaccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
    17401 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
    17461 acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
    17521 agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggttatc gaatttagtg
    17581 atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta
    17641 accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgaacatg
    17701 cgcacgaagg caaataagcc agcctgaatg agaaaaagcc aacctgcggg ttggcttttt
    17761 tatgcaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt attactgcga
    17821 atttcctgcc aaactttatc acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt
    17881 gcacgcagat actgctgata atccagtacg cggacacttt cctggtcggc aaaatgctga
    17941 tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat tttttctggc
    18001 gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacgact gtcttcctgt
    18061 ggcgcggttg attgcgcaca agctaccagc cccgatgcca ttatggcgat gagaatcagt
    18121 tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg tcgggcatca
    18181 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18241 gccgtaacca ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18301 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18361 tgcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgctaggt
    18421 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18481 ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt tagcgttgaa
    18541 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggagt
    18601 catacgttgc aggaagtatc cctgctgacc gtcagcctta acccaggtgc cttcgaggcg
    18661 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18721 gtcataaagg tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccat agaactggta
    18781 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    18841 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    18901 gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    18961 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19021 caacgcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19081 tggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa tgtaacctgc
    19141 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19201 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19261 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19321 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19381 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19441 ccagtaatat ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19501 cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg ccagcgtact
    19561 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19621 aaggtgaaca gcgaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    19681 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    19741 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcggtt
    19801 gaaaggcttg cagaagaata aaagcgtgaa attccagcaa gatgaaagcg gttacatttt
    19861 atttaattat gttttgttat tttaaattta ttttatggat aaattgcgaa gcggaattaa
    19921 ttcgcgaaga aaagcagaaa aaacgccgct gaacgcggcg ttttggattg tttgcatatt
    19981 tcttgccgga tgcggcgtga acgccttaac cggcctacaa aagcacgcaa actcaatata
    20041 ttgcagagat catgtaggcc tgataagcgt agtgcatcag gcaatttagc gtttgaaact
    20101 gtttgtctgt tccttgccgg atgcgacgta aacgccccat ccggcatagc gaaacttaaa
    20161 attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt
    20221 tttccgccgt agaatggcgg ctatcgaggc agaagtaacc ttcacgctca aactggaatg
    20281 ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg
    20341 attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc acgctgaaca
    20401 gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa
    20461 cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac
    20521 agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttctgcc ttaatgacgt
    20581 aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt
    20641 cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc tggcggctac
    20701 ccatttccgg tttgttcgga tggttcggca tggtaaccat ttcgccttcg ccctgatagt
    20761 tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggggcg ttttcgttga
    20821 gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca
    20881 cgccgatgcg tttgcagaac tcacgaatag aagcagcagt gtaaccacga cgacgcagac
    20941 cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca
    21001 ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact
    21061 ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt
    21121 cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt
    21181 gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa
    21241 atttaatgcg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt
    21301 tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg
    21361 ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg
    21421 tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat
    21481 tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt
    21541 taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca acatactcga
    21601 tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct
    21661 gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg
    21721 gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga
    21781 tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa
    21841 agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag
    21901 ttagcgaaaa tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc
    21961 agagaagatc tccaccgctt ttgccagtcc cgcgtcattg ttggatgcgc gtcttatcca
    22021 accaggactg agcttttgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcataaag
    22081 cagattactt tttgatttca tacagcggtg tttgacccgc cacaacattg ccctgagctt
    22141 taatgatcaa gccactgaaa tcgtcgatat tgctgcaaac caccgggcta atcatcgaac
    22201 gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct gcgctcacct
    22261 gcgcgccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac
    22321 ccatgtggac gacaatctcc gcgccttttt cggtttctag gcagaacgcg tggttggtgt
    22381 tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct gtcggtttca
    22441 ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcagaaacc tgatccagtg
    22501 ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg tttggtacag
    22561 cctgcggttt tgctacagga gcggcagttg ccggagttgc ttcagctgac gcagctgcta
    22621 ccgggccacg ggcaacgact ttcttcatcg catcgccaat ggattctgct ttcgcgccaa
    22681 caatcacctg aatggtctgt ttgttcagtt ttactacacc agaagcaccc agacgtttac
    22741 acatcgcatc gttgacgcga gctgagtcag ccacggtaag gcgcagacgg gtaatacagg
    22801 cgtcaatcgc tttcaggttg tcagtaccgc caaccgcagc aatatagttg gttgccagtt
    22861 gagtcagacc ttcttcagta ttgctgttag cctcttcagt tacgatctcg tcttctttat
    22921 cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac accacgaagt
    22981 agatagcgaa gaagacaaca cccatcacca gcagcatcca gacgttctgg ctggcggccg
    23041 gcaggttata catcaacgcg tagtcgatag cccccgcaga gaaagagaag ccagcatgga
    23101 tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc aggaggtaca
    23161 gcagcggagc aaggaacatg aacaggaatt ccagcggctc agtcacaccg gtcaggaacg
    23221 cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct ttcggtgctg
    23281 cgaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg aagaagccgg
    23341 acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg ccgtggaaaa
    23401 ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg ttcagcacct
    23461 gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg gaacccagcg
    23521 cgcctgcaga aacgatccac tcgccgcctg catggatagc gtgctgtact ggcggccaga
    23581 cgtaaccaaa aatggccgcc agcaccaggc agaaaaagcc ggtggcaatc ggcacaaagc
    23641 gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa cggttatagg
    23701 ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgtta atttctgggt
    23761 tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc agcgccgccg
    23821 cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt aatgcgaggt
    23881 tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa tctggctgac
    23941 cgaatcgcag caacagtgcc gccaccggca gcaccgcgat agggagctgt aacgccctac
    24001 cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa ccctatttgg
    24061 ctcgtttaaa gccgtatttt tattttgctg caaattgtac tgccgatgtt ctgtaatcag
    24121 attgttagat catctgctac agagtgtgtg aaaatttaat tcgtatcgca aattaaacgc
    24181 gtgccttttg tgagttttgt caccaaatat cgttatcatc actccctttt actggctaaa
    24241 gcagaaaact tattttatca ttcaaaaaat caggtcggat tgacgcctgt ctgcgcaaat
    24301 ccaggttacg cttaaagatg cctaatccgc caacggctta cattttactt attgaggtga
    24361 ataatgagac tgatccccct aactaccgct gaacaggtcg gcaaatgggc tgctcgccat
    24421 atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt tctgggcctg
    24481 ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca taaagcaggc
    24541 caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa
    24601 gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca cgttgatatt
    24661 ccagcagaaa acatcaacct tctcaacggc aacgccccgg atatcgacgc cgaatgccgc
    24721 cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg cggtgtaggt
    24781 aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg tactcgtatc
    24841 aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa cgatgtcaat
    24901 caggtgccaa aatatgccct gactgtcggt gttggtacat tgctggatgc cgaagaagtg
    24961 atgattctgg tgctgggtag ccagaaagca ctggcactgc aggctgcagt tgaaggttgc
    25021 gtgaaccaca tgtggaccat cagctgtctg caactgcatc caaaagcgat catggtgtgc
    25081 gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa tgaattagaa
    25141 gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggctccttgc tcagggcaat
    25201 attttttaaa atcggggggc agaatgtatg cattaaccca gggccggatc tttaccggcc
    25261 acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc
    25321 cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc attctctccc
    25381 ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac gacaccgctg
    25441 aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa tcaggctgta
    25501 ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg
    25561 ttatgcgcga gtacctggca agacatccga atcaggcgtt aggtctgcat ctggaaggtc
    25621 cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg
    25681 ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg accctggcac
    25741 cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt gtggtttctg
    25801 ccggtcactc caacgcgacg ttgaaagaag cgaaagccgg tttccgcgcg gggattacct
    25861 ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg
    25921 gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc ctgcatgttg
    25981 attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt ctggttaccg
    26041 acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt aaaacaatat
    26101 actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca tccttaacca
    26161 tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat gaagtgctgc
    26221 gtatggcgac gctctatccg gcgcgtgcga ttggcgttga gaaacgtctc ggcacactcg
    26281 ccgcaggtaa agtagccaac ctgacctcat tcacacctga ttttaaaatc accaagacca
    26341 tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag gcggacaagc
    26401 tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat
    26461 tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc ttgcccccgc
    26521 cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag aagttgatca
    26581 gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc gcaatttcca
    26641 cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc accctgtttg atctcagcag
    26701 caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaaa cgctggaaca
    26761 tgccctgttg aatgccattg ctcagtttat tgatagctac cagcgcaaac tgcgcgagct
    26821 gatcgcgatt tcggtgatcc tgccagggct tgttgacccg gacagcggca aaattcatta
    26881 catgccgcat attcaggtag aaaactgggg gctggtagaa gctctggaag aacgttttaa
    26941 agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc actacttcgg
    27001 tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa ccggggccgg
    27061 gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg aaattggcca
    27121 tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct gcctggaaac
    27181 tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc agggctacca
    27241 gagccgcgtg ccgctggacg actgcaccat caaaactatc tgcaaagccg cgaacaaagg
    27301 cgatagtctg gcgtcggaag taattgagta tgtcggtcgt catctgggta aaaccatcgc
    27361 cattgctatc aacttattta atccgcaaaa aattgttatt gccggtgaaa tcaccgaagc
    27421 cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt
    27481 tcgtactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa tcggcgcttt
    27541 tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc tggaaaatta
    27601 atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat gaccattaaa
    27661 aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt accgggtgca
    27721 gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct gaccaactat
    27781 ccttcgcaga ccgggcaaga tctggcgaac cgctttgcca ctgccggtgt cgatgtgccg
    27841 gatagcgtgt tttatacctc tgcgatggcg accgccgatt tcctgcgtcg ccaggaaggc
    27901 aagaaagcat atgtggtggg cgaaggcgca ctgattcatg aactgtacaa agccggtttc
    27961 actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacgcgttc ctacaactgg
    28021 gacatgatgc ataaagcggc ctatttcgtc gctaacggtg cacgctttat cgccaccaac
    28081 ccggacaccc acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc agggattgag
    28141 aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat catccgcgca
    28201 gcattaaaca aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga taacttgcgc
    28261 accgacattc tggcgggctt ccaggcaggt ctggaaacga ttctggtgct ttctggtgtt
    28321 tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta cccgtcagtc
    28381 gctgaaatcg acgttatctg aataccaccc acgtctgcgg acgtggttgg ctctgatgac
//
LOCUS       DAJTVE010000044        27420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000044 DAJTVE010000000
VERSION     DAJTVE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..135
                     /locus_tag="OO439_001811"
     CDS             <1..135
                     /locus_tag="OO439_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6316192.1"
                     /translation="DRPDIAVRYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG
                     "
     gene            complement(266..1589)
                     /locus_tag="OO439_001812"
                     /pseudo
     CDS             complement(266..1589)
                     /locus_tag="OO439_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1589..3205)
                     /locus_tag="OO439_001813"
     CDS             complement(1589..3205)
                     /locus_tag="OO439_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6316193.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3501..4217
                     /locus_tag="OO439_001814"
     CDS             3501..4217
                     /locus_tag="OO439_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6316194.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4214..5875)
                     /locus_tag="OO439_001815"
     CDS             complement(4214..5875)
                     /locus_tag="OO439_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6316195.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6071..6499)
                     /gene="ibpB"
                     /locus_tag="OO439_001816"
     CDS             complement(6071..6499)
                     /gene="ibpB"
                     /locus_tag="OO439_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6316196.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6611..7024)
                     /gene="ibpA"
                     /locus_tag="OO439_001817"
     CDS             complement(6611..7024)
                     /gene="ibpA"
                     /locus_tag="OO439_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6316197.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7018..7090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7330..7662
                     /locus_tag="OO439_001818"
     CDS             7330..7662
                     /locus_tag="OO439_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6316198.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7664..8878)
                     /locus_tag="OO439_001819"
     CDS             complement(7664..8878)
                     /locus_tag="OO439_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6316199.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8979..10043
                     /gene="cbrA"
                     /locus_tag="OO439_001820"
     CDS             8979..10043
                     /gene="cbrA"
                     /locus_tag="OO439_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6316200.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10040..11377)
                     /locus_tag="OO439_001821"
     CDS             complement(10040..11377)
                     /locus_tag="OO439_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6316201.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11452..12600)
                     /gene="dgoD"
                     /locus_tag="OO439_001822"
                     /pseudo
     CDS             complement(11452..12600)
                     /gene="dgoD"
                     /locus_tag="OO439_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12597..13214)
                     /gene="dgoA"
                     /locus_tag="OO439_001823"
     CDS             complement(12597..13214)
                     /gene="dgoA"
                     /locus_tag="OO439_001823"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6316202.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13198..14076)
                     /gene="dgoK"
                     /locus_tag="OO439_001824"
     CDS             complement(13198..14076)
                     /gene="dgoK"
                     /locus_tag="OO439_001824"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6316203.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14073..14762)
                     /gene="dgoR"
                     /locus_tag="OO439_001825"
     CDS             complement(14073..14762)
                     /gene="dgoR"
                     /locus_tag="OO439_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6316204.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14731..15363
                     /locus_tag="OO439_001826"
                     /pseudo
     CDS             14731..15363
                     /locus_tag="OO439_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15409..16221)
                     /gene="yidA"
                     /locus_tag="OO439_001827"
     CDS             complement(15409..16221)
                     /gene="yidA"
                     /locus_tag="OO439_001827"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6316205.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16336..16734)
                     /locus_tag="OO439_001828"
     CDS             complement(16336..16734)
                     /locus_tag="OO439_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6316206.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(16974..19388)
                     /gene="gyrB"
                     /locus_tag="OO439_001829"
     CDS             complement(16974..19388)
                     /gene="gyrB"
                     /locus_tag="OO439_001829"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6316207.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19417..20490)
                     /gene="recF"
                     /locus_tag="OO439_001830"
     CDS             complement(19417..20490)
                     /gene="recF"
                     /locus_tag="OO439_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6316208.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20490..21590)
                     /gene="dnaN"
                     /locus_tag="OO439_001831"
     CDS             complement(20490..21590)
                     /gene="dnaN"
                     /locus_tag="OO439_001831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6316209.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21595..22998)
                     /gene="dnaA"
                     /locus_tag="OO439_001832"
     CDS             complement(21595..22998)
                     /gene="dnaA"
                     /locus_tag="OO439_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6316210.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23295..23375
                     /locus_tag="OO439_001833"
     CDS             23295..23375
                     /locus_tag="OO439_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316211.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23605..23745
                     /gene="rpmH"
                     /locus_tag="OO439_001834"
     CDS             23605..23745
                     /gene="rpmH"
                     /locus_tag="OO439_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6316212.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23762..24121
                     /gene="rnpA"
                     /locus_tag="OO439_001835"
     CDS             23762..24121
                     /gene="rnpA"
                     /locus_tag="OO439_001835"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6316213.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24085..24342
                     /gene="yidD"
                     /locus_tag="OO439_001836"
     CDS             24085..24342
                     /gene="yidD"
                     /locus_tag="OO439_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6316214.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24345..25991
                     /gene="yidC"
                     /locus_tag="OO439_001837"
     CDS             24345..25991
                     /gene="yidC"
                     /locus_tag="OO439_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6316215.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26097..>27420
                     /gene="mnmE"
                     /locus_tag="OO439_001838"
     CDS             26097..>27420
                     /gene="mnmE"
                     /locus_tag="OO439_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6316216.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDD"
BASE COUNT         6586 a         7440 c         7020 g         6374 t
ORIGIN      
        1 gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat
       61 tttgccaata gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag
      121 caaacacctg gttaggtgga aaaccgcgcc ttatatcccc gccctgaagg acggggtttt
      181 acggcgcacc ggataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
      241 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
      301 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgataacg
      361 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
      421 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
      481 gtggttctgg tccgttgtgc ccgaccagac acgggatctc gaccatcgct tcatcatcaa
      541 agttatggat tgccccgttg ttaggcacaa tcagcaacat ccgctcctga gtgttgaagg
      601 caattgccgc cgccagatcg acgatgtatg acgcatgttc gtcaatctcc agcttggccg
      661 ccgctgaatt tcctgccgct gtaatggcgc ggcaagcatc gaaaacctgt ttctcgcgat
      721 gttccattca cctcattcgc gcgggtatgt tcagggttgg aatgctgaac aacataatcc
      781 gggaagagat aatatttcag ataggtgttc ggcaatgtat ccgggtccag tgcctggaca
      841 tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag tatgtgtatc ttgctgcggt
      901 ggaacataac cgtgcttcga gacatattgc cgcagctggg gcatcaggtc gttgccctgc
      961 aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc cgtagtagcg cacgcgcatc
     1021 tgtttgcgat cttgcagccc aacaatttgc gccatccggc tttcaatacc gattggcatg
     1081 tcacagatgt tgaggatttt cgcattcggg cgcagacgac gcgtggcttc tgcgacaatg
     1141 gctgccgggt tggagtagtt gagcatccag gcatttggtg aatatttttc catataatcc
     1201 accagttcca ggacgccgcc aatggaacgc atgccgtacg ctattccgcc gggtccgcaa
     1261 gtttcctgac caacaacacc gtggcgcagt gggatttttt catccagttc gcgcatcggg
     1321 tacttgccga cgcggatgtg cgccatgaca aaatcaacgt cgctgaacgc cacttcagga
     1381 tcggtggtgt aactaaacgc aatgtccggc gctttctctt taaggatgac tttacaggct
     1441 tcggcaatca cttcctgccg cgcgccatcg ttatcataaa atttcagtgc acgaagcggg
     1501 aaacgctcct gattcgccag gagcatcaac acgatgcctg gcgtaaaggt gcttccaccg
     1561 cctgcgacaa ccactgagaa tttggtcatt atactgcctc cgtaatggca acattttctg
     1621 ctgattgatg agaattaatt aagctatcga gctgttcacg cagctgggat acatgcagac
     1681 caatgatcac ctgaatggca tcgccactac ggaagacgcc gtgcgctccc agctttttaa
     1741 agacttcgtc atccagcgtt tgtgacatgt catgcagtgc aatgcgtaga cgcgtcgcac
     1801 aattgttaat gctggagata ttgccgaccc cgcccagggc ttgcaggata ccggcagcct
     1861 gatccagctc tttttttggc tctgccgcgg tggtttggcc tcgcgaggct ttgtattcgg
     1921 cttttgagta gagtttcact tccgcatctt cacgtcccgg cgtgcacatg ttgaactgca
     1981 gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa gcacaaccca atggcgattt
     2041 gggtcagcat catatccgcg tggttgctga acatcgggat ccagttttgc ggtaaaacct
     2101 ggtcaatcag acctccgccc atgttgccca ccacaccaaa gagatacatc acggtcgaca
     2161 ttgaggccgc aagcacagcg tgtaccgcaa acagcaacgg tgaaatgaac aggaaggtaa
     2221 attccagcgg ttcggtaatt cccaccagca tggcggttaa ggttgcggga atcagcaagc
     2281 ccgccacttt tacccgattt tccggtgcgg cagtgaagta catcgctaaa gaaatgccca
     2341 cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc gccttccggg aacaacgatt
     2401 tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg cgcccagtac atctgaatgc
     2461 cgccttcaac agctgccgga cccaagataa acggtccgta gatgaagtgg tgtaaaccgg
     2521 ttgggatcag aatacgttcg aggaaggtgt aaacccagac cccaagcgca cccgccgaac
     2581 gcaggaacgc ttgcagagat tcaatccccg tttgtacttt tggccagccg agcaatgtca
     2641 gccaggcaca ggggatcatc accaggaagg cgataatcac cacataagac gtcccctgga
     2701 aaatgccgag aaaaaccggc agttttttat cgaacagacg gttatgcagc gccgtcacaa
     2761 tgccggaaat gataattgcg ccgataatgc tggtatcgag ggttttaatc ccggccatca
     2821 ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc gacgccgaag tagcttcccc
     2881 aggtcattcc catcgcgttg atgaaatagt tccaggtcag gaaactcacc atcaccgcca
     2941 gacaagcacg tccctgcgct tgcttagcaa ggccaatggg taaaccgaca gcaaaaatca
     3001 gcggcatatt acggaatacc gtccaaccgc cctcttcaat aatgtgtacg atttgcgcga
     3061 ataaactgtt cggatcggtc agtgattccc cgacaaacat cgggttttgc agcaagatgg
     3121 caagacccac cacaatcccg gcgaagggaa acagcagcac tggcgtaaac atcgcgccgc
     3181 caaagcgttg aatttgactg agcatttcca catcctcttt tctcaattct gtaagtagga
     3241 agcctgattg gttatgcggc aggcctggag caaggatagg aagatgcgat cggcagaaca
     3301 tgacgaccca atgggattcg tgatcgcttt catgcttttc gttgggcggt agagatctac
     3361 gttttcgcga aaaagtagac atctgtgagc acaggtacac agcatcgaac acatctttaa
     3421 aaaaaagatg ttttttcaat cgattaagca gaacttgtgt gcgcattacc cgggcttgca
     3481 ggcaaaaaag agatctagag atgatctaca aaagcattgc ggagcggtta agaattcgac
     3541 ttaactccgc agatttcacg ctaaacagcc ttcttccggg tgaaaaaaag ctggcggaag
     3601 agtttgcggt atcgcggatg accatccgta aagccattga cctgctggta gcgtgggggc
     3661 tggtggtccg ccgccacggt agtggcactt acctggtgcg caaagatgta ctgcatcaaa
     3721 ccgccagtct gaccgggctg gtggaggtgt taaaacggca gggaaaaact gtcaccagcc
     3781 aggtgctgat ttttgaaatc atgcctgcgc ctccggccat tgccagccag ttacggattc
     3841 aaatcaacga gcagatctac ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctga
     3901 tgctggaaga cagctatatg ccggtaaaac tgttccgtaa tctttcgctg caacatctgg
     3961 aagggtcgaa gtttgaatat attgaacaag agtgcgggat tttgattggc ggtaattatg
     4021 aaagcctgac gccggtgctc gccgatagac tgctggcgcg gcaaatgaag gtagcggaac
     4081 acacgccact gctgcggatc acctcgttgt catatagcga gagcggggag tttttgaatt
     4141 attcagtgat gttcagaaat gccagcgaat accaggtgga gtaccattta cggcgactcc
     4201 acccggaaaa gagttaaccg atactccaga agagcaccgc cagtaattgg ggggtgataa
     4261 tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgcc gcaccgctag
     4321 ttggatgaag attattagca aacgccagcg ccggaggatc ggtcatggaa cctgccagca
     4381 tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaatg ccaacagtaa
     4441 tcagcggaac ggcggtgatc agggcaccat aaccaatcca gcttagccct tcgccattga
     4501 ccagggtatt cacaaaatcc ccaccagatt tcagaccaac gaccgagagg aacagcacga
     4561 tccccagctc ccgcagcgcg aggttggcac ttggcggcat aaaccagtac agcttgccga
     4621 tactgccgat acgcccgagg atcaacgcca taatcagcag accgcccgcc agccccagtt
     4681 tcaacgcggc cgggaatccc ggcacaaaga cgggaataga acctaacaat acgccaagcc
     4741 cgatgccaat aaacactggc agcatctgaa cctgttgcag tttttgctgc gcattcccca
     4801 gcacattggc aacggcatca attgcggacg gacgccccac caggttgagg atatcgccga
     4861 actgcaggct gatatcgcca ctggcgacca gttcgacccc ggcacggttc aggcgcgaga
     4921 taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt
     4981 cattggtgac caccacacgc tcgacgcgca aatcagtgtc tttcgtggac agcgaagtat
     5041 cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgctggctga cccaccagat
     5101 gcagcaaatc gcccagttgg ataatggtat ctggcgaagg aacttttagg gtttcttcgc
     5161 gtttcagacg cgagcagata attttgtcgc cgttgagaat cggtacatct ttaatggcta
     5221 aatcatgcag gttagggttc tcaacgcgaa tattgatagt cttgatcagc gcgccgccat
     5281 tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac attgacgcgg aaaataaccc
     5341 gcaacatcca catggtgaac aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac
     5401 tcatccccat ctgatcgacc atttccattg gtgtacccag gtcgcgcaaa atctgctgcc
     5461 ctgcccccag cgctggcgta ttggtaaccg caccggagaa aatccccagc actaccggca
     5521 gtggaatatc aaacagttta tgcaggatgg cggtaaccag accaccgatg atgacgatca
     5581 gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc
     5641 ctacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga ataacatgca
     5701 gcatatcgct acttaatgtc atccccgcct gagaaacaaa atggccgacg atgatcccac
     5761 caaacagcac gccgccaata cctaatccta tgccgcgaaa tttgacgttg ccgataaaca
     5821 aaccgacgac tgccaccaaa gccagaatac tgaccgttaa tgctatatca ctcatcgtcc
     5881 atttctcttg agataacatt ttaattgcta cggattatgg ctgaatcggt gaagcagatt
     5941 tgccttatag cgcacattat tacgggaaag aacgtgccga aatatcttaa acagtcgaaa
     6001 tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga
     6061 gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc tgcgctgcga
     6121 tgggttcagg ctcattacga attaaatcaa tatgcagtaa accgtttacg aaagttgcgc
     6181 cagagacttc catattttca gccagcgtaa agctcaggct aaatggctga ttcataagcc
     6241 cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt acgctcaggc
     6301 gcgtaccttc cagttgaatc tctaaatctt cctgacggaa acctgccagc gcaagggtaa
     6361 tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc tgactttcac
     6421 cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc atcagtgggg
     6481 ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt acctgcaaat
     6541 ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg cgccaggcgg
     6601 ccttaaggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga atcacgcgtt
     6661 cgagatcgat atacagcaaa ccatttacca ggttagcgcc acgaacatga atgttctcag
     6721 ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct
     6781 ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg gcggtaattt
     6841 ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg ttttcgtcta
     6901 ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg ttttctaagt
     6961 ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa tcaaagttac
     7021 gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat ggacaggtca
     7081 ggtagccaga acacccatca ggcgcgtcct catcgactac gatgtaaaaa tgggtctgga
     7141 aatgaacttt tcaagcctta tcagcggact tttttttgca gtttatggtc tattgcatag
     7201 actgaggggg cagcacgaaa cgttaaagtg tgatagccgc cacagagcga cgtaatgatg
     7261 atgttatttt cagcaaggga tcgctataac tcatcaggcc aacacccacg atcaacagat
     7321 gaatttatta tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg aatggcatta
     7381 ctcggaggat gctccagcgt gatgtcccat accggaggga aagaaggaac gtatcccggc
     7441 acccgcgcca gcgccaccat gattggcgat gatgaaacca actggggcac taagtcgctg
     7501 gcgattctcg atatgccgtt taccgctgtg atggacacgc ttttattacc gtgggatgtg
     7561 ttccgcaaag acagctcggt gcgctcacgc gttgaaaaaa gcgaagccaa cgcgcaagcc
     7621 accaatgcgg ttattccacc ggccagaatg cctgataact gatttagcga tcagtttcgg
     7681 tgatccacag ctggccacct tccatccacg ccagccattg accatccggc gagaagacca
     7741 cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa atactcaacc tcgccgcttt
     7801 gcgcatgggc acaagcaatt cgattatcca gcacaaagcc caaccattct cctgacggat
     7861 gccagttaaa tgcagactga atatccgttt tgttatgggt taactggcgc ggctcgccgc
     7921 cctgtggcga gataagccac agttgcacaa tgccgttatc atcacgcatt aaaaacgcga
     7981 tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg gacgttgact aaccccggat
     8041 aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac gccacgcggt ggcgcgggca
     8101 gggtggtttc cgttccactt aacggcgcat cacctgccgc tttccagcca gcttcatctt
     8161 gcggcaactc aacgataaac agctccggca ctttctcacc ctttggcgaa agtgtgtcgc
     8221 caataaacgc cagcgcgtga tttcctaccc atccttcttc ataagcacga ttgatttcat
     8281 cactgccagg ctgcggcgtg ggcgtggttt tactcaccag cacacaccag tggctgccgc
     8341 tgtattcacg cggatgctgc ttttgtacgt tgaccggacc aaacggcgca gcaacaccga
     8401 cgtttcgcaa atccagcgcc ggatcgagtt catgcattac atggtcgtta taggtaaagc
     8461 tcaccctttc accgttcggg ctaaagacat gcacatggct gccgccgcgc agcgctcctg
     8521 gcgtatacgg cgcggtaata tccattgcat cgagattgct caccttgccg ccttcagcaa
     8581 tgactccgcg ccgatgatgg aagtcgtaat gccatgtttc atcaggattt tccgggccgt
     8641 gaataaagac atatttctct gacttcggat gaacggtcac cacgccgacg tgtgcgccct
     8701 gtgacgcaca gtagataacc tcgacctcac cggtatggat attcacacgc tcaatggtct
     8761 cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg
     8821 tccaggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc tgtttcatgg
     8881 cgcggtcctg atacaaaacg ggtccagcca tcatacttca caaggacttc actttcagac
     8941 aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc gacgtggcga
     9001 ttattggcct cggcccggca gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg
     9061 tgatcgcgct ggataaaaag caccagcatg gtactgaagg tttcagcaaa ccttgtggcg
     9121 gtctgctggc accggacgcg cagcgatctt ttattcgcga tgggctgacg cttcctgtcg
     9181 atgtgatcgc taatccgcag attttcagcg tcaaaaccgt cgacgtcgcc gcatcgctca
     9241 cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac ttgtggatga
     9301 aatcgctgat ccccgccagc gttgaggttt atcacgacag cctgtgtcga aaaatctggc
     9361 gtgaggatga taaatggcat gtcatttttc gtgcagacgg ctgggagcag catattaccg
     9421 cccgctatct ggtcggtgcc gatggtgcca actcgatggt gcggcgacat ctctacccgg
     9481 atcatcaaat ccgtaaatat gtcgcaatcc agcagtggtt cgcagagaaa catccggtgc
     9541 cgttctactc ctgcatcttt gataatgcga taactgactg ttattcatgg agtatcagca
     9601 aagacggtta ttttatcttt ggcggtgcct atccaatgaa agacggtcag acgcgtttca
     9661 cgactctgaa agagaaaatg agcgcctttc agttccagtt tggcaaagcg gtgaaaagcg
     9721 aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt tgtctgcggt aaggacaacg
     9781 cctttctgat tggtgaagcg gcgggattta tcagcgccag ctcgctggag gggattagct
     9841 atgcgctgga tagcgcagac attctgcgtt cggtgttact gaagcagcca gagaagctca
     9901 ataccgctta ctggcgcgcc acccgcaaac tgcgtttaaa actcttcggc aagatagtaa
     9961 aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt ggtgtggcac
    10021 atattccaca gttgaaggat tagccaacgc gcttcacatc gcccaccagc aggatataag
    10081 agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggtgcg aaaccgtaac
    10141 cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca ccggcgaagt
    10201 taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagaa accagcgacc
    10261 aggtaattga agcaaaaccg ttaccgaaga atgccagcgc catcaggcac ataatcatca
    10321 tcggatcgtt agtgtagtta gcgcccataa tgcaggtgga gatcagcaag ccgcagataa
    10381 tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga tccgctaccc
    10441 agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc ataaagcctg
    10501 ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa
    10561 accacagtgt agaagccacc gcaaattgcc caagatagac gccgatcagt ttacgatgga
    10621 acaccagttt ccagtctttg gctgttaacg gctgacgtgc ctctttcttc actggcgcat
    10681 caccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg ctgatgcctt
    10741 tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata atgccgatac
    10801 caccagtcac aatgaacacc cagtgccagc tcaacatctc ctgaatccag atcagcagcg
    10861 gagtcaaaaa cgccagaccg acaaactgac cagacgtata aaaaccaacg gcggaggcgc
    10921 gttcatgttc cgggaaccag ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt
    10981 cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat taatcccgtg gcaaagccct
    11041 ggaataaagt cgccactgac cagccaaata tcgcaataaa ataagtcacg cgagaaccta
    11101 cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt ataaagccag gcgaaggccg
    11161 aaaatacata gcccatttcc gctttcgtaa tgccgaactc ttcctgaata tgggcggaag
    11221 ccacggccag gttagcgcgg tcaacataac aaatgaccac cgtaataaag atcatcacca
    11281 gcgtcagata acgccgacgc cccggctttg ctgcattaac gggaatatcc atagcgagct
    11341 ttctccagat tttgggcata gcgaagccgc tcaccatgcc ctgtaattta cagagggtta
    11401 tatttttgta ttgctgtttt agtgcccgat gaggggctta cgtggcagga attaccactc
    11461 tgctacgctg ttatcttcat gacgccagag tggattacgc cagtccgggg catttttact
    11521 gaactcaata actttagctt cgtcgatttc cacgcctaag cccggtttcg ttagcggttt
    11581 aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc acaaagtcga gtaactccgc
    11641 gcctttgttg taatgaattc ccatactttg ttcctgaagt accgcgttat aggaaacaaa
    11701 gtcgatatgc agacaagccg ccagtgcaat cggtccgagc ggacagtgcg gcgcaagggt
    11761 cacgtcatag gcttctgcca ttccggcaat tttgtagcat tcggtaatac cgcctgcatg
    11821 ggagagatcc ggttgtagaa tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa
    11881 acgtgagaac atgcgctcac ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg
    11941 gtagtattcg gcttgttccg ccagcaccgg ctcttcaata aacagcgggc gatacggctc
    12001 cagttcttta atcagcactt tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc
    12061 aaactcaatc tgattgccaa aagcttcacg aatttgtgcc acggtgttga ctgccgcatc
    12121 taccgcgcgg gagttatcaa ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc
    12181 gaagccgatt tcgcgcaggg ttttaatacc gtcgatcaca tccgccggac gatcgccgcc
    12241 gacccaactg taggctttaa ttttgtcgcg aaccaggccg cccatcagtt gccagaccga
    12301 cgcattcagc actttacctt tgatatccca taacgcctgg tcgatcccgg caatggcgct
    12361 catcaggatt ggaccgccac gataaaatcc ggcgcgatac atcacttgcc ataagtcatt
    12421 gatgcgctat ggatcctgac caatcaaata gtcacccagc tcgtgaactg ccgcttccac
    12481 cgtgcgggcg cggccttcaa tcactggctc gccccagccg acgacgcctt catcggtttc
    12541 aattttcagg aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat
    12601 tgcactgcct ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg
    12661 gactgcccgg cgcgatagag atcgctgcct aagcccgccc cagcacaacc tgcgtctatc
    12721 cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccgatggc
    12781 aataccgctt ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg aaatattttc
    12841 agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg
    12901 cagacggtca tgccgtagcc caccgcacgg cggatcactt cactatggat attgggcgta
    12961 acgatgagct gacagcccat cctggcgagc gcatcgacct gttcaggttt cagtaccgta
    13021 cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctttgctcc
    13081 cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca
    13141 acatgcgcca gcgcctcgtc gggcttaatg ccgcgcaaaa tggcgatcag cggaagttta
    13201 gtttgccact gcatgagcga tgctccttat accagcctga aatgccgtgt cgcccgccac
    13261 cgccgtcacg tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg tcaacgatgt
    13321 tccggcgaca agggtgatgg cgtgttgatg ggccacatag tcgcgcatac tggcgacctc
    13381 tgcgccaatc aacaaaccag agagaaattc actgacctgt tcacggggaa gtgttcccag
    13441 cacatgcgag gcacgaactt caaaaagctg cggcaatatg gcgggcgtat taagaccacg
    13501 ctcaaggcca gccgtgaagg catcggcaga gttttcctgc ggcggcaaac ctgcaccaat
    13561 caatgagtga tttaacagta aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt
    13621 gatttgctgg ctatcggcct gcacccattt gcaatgcgta ccgggcatga cataaagaga
    13681 ggaaggagcc agagctcgcg cgccgatcaa ttgagtttct tcgccgcgca tcacattgtg
    13741 gttatcatca tgagagacgc ataatccggg aataatccag atattgtcgc caacagacgt
    13801 taattgttcg ccaatagacg aaaaatgggc aggaacagat aaatacggag caactttcca
    13861 gccgacattg ctgccgacca ttcctgccat taccactggc gtattctctt cacgccagtc
    13921 ggtcgtgact tctgctaaca ccgcagccgg agattttccg ttcaggcgcg tgacgcctgc
    13981 ttctgattgc ctgctctcca ggcagtggtc gccctgataa agccaggcgc gcagattggt
    14041 cgatccccag tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc
    14101 gagctggcga tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc
    14161 gcatcgaaca gcgccttatg ttcctggagc gtttgcggca tgttggcctc atcgcccatc
    14221 caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg caatgctaag ttgctgtaac
    14281 accgggttat gcaccgactg cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac
    14341 gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga
    14401 tcgctggagg tcgcgcgctc tgctgcccaa cgggcaatcg ccggttccac cagatttcgc
    14461 acttcgctca tggcactgat aagccgtggg tcgtagtcgt tttccagcac ccattgcagt
    14521 acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca caaacgcgcc gcgataacgt
    14581 ttcatttcaa tcagccgttt cgccatcaac gaacggaaca cctcacggat gatgttgcgc
    14641 gaggttgcaa actcctcaca gagttccgcc tcagccggaa gcggcgagcc tggtacgtat
    14701 ttgccgtgaa cgatctgttt acccagcgta atgacgatgc gatcggtttt attgagagtc
    14761 atggagagtc cttgtgctct gtatgttctt ctctacttta ccccgatcga tgcataatca
    14821 ggaaagtatc aatcgcagtc agacaccatc acggggctat cgcaggcaaa ggatagtaat
    14881 ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt accttatcac agatggtgcc
    14941 gatgacatcg ttaagatgct caaggatcca gctcttaacc ggcacaatat tcaggttgct
    15001 gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac aatatcactc
    15061 tattacaact ggagcacaga agaagaaaaa gcacgagtga cacaatatcg ttttgcttgt
    15121 ggtgttcagc actgtacccg gacagtagaa aacctggaag gtactatcca tgagaaaaat
    15181 aaaaacatgg attactcaaa cgtaatggct ttctaccatc catttaaagt gcgattttat
    15241 gaataccatt cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact
    15301 gttacgctgg acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa
    15361 taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac
    15421 atacttctca atagcaaacg ccacgccatc ttcaaggtta gatttggtga caaagttcgc
    15481 cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat
    15541 cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa
    15601 cacgtcggcc agtgatttca cacccgtacc tttgttaacg cgtttatcga ggatttcgag
    15661 gaagtacggc gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc
    15721 gatagcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt
    15781 ggggtccatt ttctccgctt cgcagaacac cagcggaata gtggcaacga aagattcatg
    15841 cactgtgtag tagctgatat cgcggttggc ggtgtacagc gtggtgcggt ccagggcgtg
    15901 gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt catagctgag
    15961 agcagtttgc gccacggtgc taccatcagc tgccttctgt actagcgcgc cgttataagt
    16021 aatgcagtag tcgcccggct gttccatatg cagctctttc aggtagttgt gcacacctgc
    16081 atacgggcga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc
    16141 atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat
    16201 agcaatgagt ttgatagcca tgagttcccc aggtagattg gttcctgacc catgctaacg
    16261 cgattccgct caaaaatcag tacaacaccc gagggaaaag ggggatgcaa cgcgggttgc
    16321 atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatac ctgcggaaag
    16381 cagatcgtta ttggcttgtg gtgacacttc accttgcggt gagagcgcat caataatctt
    16441 cggcaattgc tctgccagta aactggaagc tgtactggta tccacgccaa gtttttgccc
    16501 gagatcggac accgcatttg tgccgagcgc cgattccagt tgctcgccac taaccgattg
    16561 attgccctgt tgattactca gccaggttga gagaatggcc cctaagccgc cactttgcag
    16621 tttttccagc agcacctgaa tgccgccctg ctcctcaacc caacttaaaa tagcctgata
    16681 tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc ccatgataat
    16741 cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg ataattgcgg
    16801 attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct tcttgccgga
    16861 tgcggcgtga acgccttatc cggcctacaa aatcatgaaa attcaataca ttgcaagatt
    16921 ttcgtaggcc tgataagcgt agcgcatcag gcacgctcgc atggttagcg ccattaaata
    16981 tcgatattcg ccgctttcag ggcgttctct tcaataaacg cacggcgcgg ttcaacggcg
    17041 tcgcccatca gcgtggtgaa caactggtcg gcagcaatcg catctttaac ggtaacgcgc
    17101 agcatacgac ggctttccgg gtccatggtg gtttcccaca gctgttccgg gttcatctcg
    17161 cccagacctt tataacgctg gatggagagg ccgcgacggg actctttcac cagccagtcc
    17221 agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat aaacgcatct
    17281 tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata ttcgccaccg
    17341 gtgataaact cgtgatccag cggatagtca gtatccacac cgtgggtacg cacgcgaaca
    17401 atcggttcaa acaggttttg ttcggcattg gtgtggacat caaacttcca ctggctgccg
    17461 tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt aacggtctgc
    17521 tcatcagaga ggtcggcttc cgtcagcgtc ggctgataga taagctcttt cagcattgct
    17581 ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta ctcagacacc
    17641 aatttctcta acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg cagcgttgcg
    17701 ccatccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt aatgtactgt
    17761 tcttgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac gtgaccgcgt
    17821 tcaacgattt ccggcatctg acgatagaaa aaggtcaaca gcagcgtacg aatgtgcgag
    17881 ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt gtccgggttg
    17941 tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac ttcctgagaa
    18001 gagagcatct tatcgaagcg cgctttctca acgttgagga ttttaccctt cagcggcaga
    18061 atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga gtccccttcc
    18121 accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag tttgcccggc
    18181 aggcccgcta agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg acgcgccgct
    18241 tcacgggcac gggcagcatc gataattttg ccaaccacga ttttcgcgtc ggttgggttt
    18301 tccagcaggt attctgccag cagttcgttc atctgctgtt caaccgctga tttcacctca
    18361 gaagaaacca gtttgtcttt ggtctgggag gagaatttcg ggtccggcac tttcacggaa
    18421 acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg cgctaacttt ggcttttttg
    18481 ctgtagcctt ctttgtccat gtaggcgttc agggtacggg tcatcgccgc acggaagcct
    18541 gccaggtgag taccgccgtc acgctgcgga atgttgttgg taaagcagta gatgttttcc
    18601 tggaagccat cgttccactg caacgccact tcgacgccaa taccgtcttt ttcagtggag
    18661 aagtagaaga tattcgggtg gatcggcgtt ttgttcttgt tcagatattc aacgaacgcc
    18721 ttgatgccgc cttcatagtg gaagtggtct tctttgccgt cgcgcttgtc gcgcagacga
    18781 atggaaacgc cggagttgag gaacgacaac tcacgcagac gtttcgccag aatttcatat
    18841 tcgaactcgg tcacattggt gaaggtttcg aggctgggcc agaaacgcac catggtgccg
    18901 gttttttcag tctcgccggt aaccgccaac ggtgcctgcg gtacaccgtg ttcgtagatc
    18961 tgacggtgaa ttttaccctc gcgctggata accagctcca gtttttgcga cagggcgttt
    19021 actaccgaaa caccaacgcc gtgcagaccg ccggacactt tataggagtt atcgtcaaat
    19081 ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc ctcttccggg
    19141 tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag agacagagtt atcggcgtga
    19201 atggtgacga taatttcttt acagtgaccc gcgagcgctt cgtcgatagc gttatctacc
    19261 acctcgaata ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat atacataccc
    19321 gggcgcttac gcaccgcatc cagccctttc aggactttga tactggagga gtcataagaa
    19381 ttcgacatca acgtttctcg ctcatttata cttgggttaa tccgttattt tacccttttc
    19441 cacggtaaac atcttcgaat tttcgtccga catgtctata acgtgttcag cactgatcgc
    19501 gctgacaaag acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc cgcgacgctc
    19561 atcatcaagc tcagaggcaa aatcatctat caggtagaga caccgccgcc cgctttcacg
    19621 ggtgaggaac tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa gctgcccacg
    19681 cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg ctttatgcgg
    19741 gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca gcacctcagc
    19801 atattctgtc tctttctccc agccgcgctg gaaagagaaa gtcagagaaa actcagggag
    19861 aaattgctta caagtatcgg ccatatcagc cgcgataccg gcgctatact ccgcgcgcca
    19921 ggtgctgatt tgctccgcca gcgggatcag ctctttatcc cacgggcgta gctgttcgta
    19981 acgtgtcacc tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga gattgctcca
    20041 ggcggtgaaa aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg ctcttctgta
    20101 tttggggccg ccgttgagta aagtaaaccc ttctggcgtt atcaactgca ttggcatcag
    20161 gtgcgccagt tccgcgacct tatgcccgtc tgtaccgtcg atgcggactt tgctgtcgcc
    20221 ctgtttgtct ttggttaagc caatcgctgt ctcgcgctct tcgccctgta atcgcccgtg
    20281 gagaacaaac gcctcctgct catggcgaat gacgcgacca atctgcaaac tgcgaaacgc
    20341 ccgaccatgg ccgagcgtat agatggcttc cagcacgctg gttttgccac tgccgttggc
    20401 acctaccaga aagttaaagc cgggagataa agcgagatcc gcggtttcaa tgttgcggaa
    20461 atcgcggatc aacaagcggg tgagggacat tacagtctca ttggcatgac aacataggca
    20521 gccgactgtg atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt cagcatcatg
    20581 cggacgtttt cacatttcag cgcgttcaga acatccagca cgtagctgac gttgaagccg
    20641 atttccatct ccgcaccgct ataggtaacg tcgaggatct cttccgcttc ttcctgttcc
    20701 gggttgttgg cggtgatttt cagctggttt tcgctgacat aaagacgtac gccgcggaat
    20761 ttctcgttag agagaatcgc cgcgcgagca aacgcctgct tgagcagatc gcagccagct
    20821 tccagatgtt tgtccgggtt cttcggcaga acgcggcgat aatccgggaa gcgaccatcc
    20881 accagtttgg aggtgaagat aaagtcgcca acgtgggcgc gaatgttgtt gctgccaatc
    20941 tgtacgcgca gcggattgtc gccgccgtcg agcatacgca tcagttcaat cacgccttta
    21001 cgcggcacga tcaccgaatg gcttggcaaa gattgaccaa ttggcattga acagactgcc
    21061 agacggtggc cgtcggtcgc cacggtgcgc agttcttcgc cttcggtttc aaacagcata
    21121 ccgtttaagt aatagcgaac gtcctgatgc gccatagaaa actgggtcgc ttcaatcaga
    21181 cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc atcgaggttc
    21241 gggaaatccg ccgctggcag ggtagacagc gaaaaacggc tacgcccgga gcgcaccagc
    21301 atccgttcac cttccagctg tacggcaatt tccgcgcctt caggcagacc acggcagata
    21361 tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg aaccagcgca
    21421 acgcgtgcca ccatttccat ctcgagatca gtaccggtca gcgacaacgt accgtcagca
    21481 acctgtaaca gcagattacc gagaatcggt agcgtaggac gaccacctaa cggcccgctc
    21541 acctgttgta gcggttttaa taaatgctca cgttctacgg taaatttcat aggtttacga
    21601 tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata tcgtggctct cttcacgcaa
    21661 ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg tcacgaccac caaacgcatc
    21721 gccaatctcc ggcagactgt ggttagtcag ctctttcgcc agcgccatcg ccatctggcg
    21781 cggacgcgcc accgagcggg atcgacgctt ggaaaggaga tccgcgactt tgatcttgta
    21841 gtactccgcc accgtcttct gaatattgtc gatggtgacc agtttttcct gcaatgccag
    21901 caagtcgcgc agtgcctcac gcacgaagtc gatggtgatc gcccgtccgg taaagttggc
    21961 gttagcaata acgcggttca gcgccccttc cagctcacgt acgttagatc gtagacgctt
    22021 ggcgataaag aacgccactt cgcctggcaa acgaatgtca ttttcgtcgg cctttttcat
    22081 caggatcgcc acgcgggttt ccagctctgg cggttcgatc gccacagtca gtccccaacc
    22141 gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc gatccgaggt
    22201 gagaatgatc tgttgattac cttccagcag ggcgttgaag gtgtggaaaa actcttcctg
    22261 agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat ctacggaacg
    22321 gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg gctttaacca tgtcctgaac
    22381 aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca taatgccgtt
    22441 acccaccgca tgcagcaggt gagttttacc cagacccgtg ccgccataaa ggaacaatgg
    22501 gttataggca ccgccagggt tatccgccac ctggcgagcc gccgcgcgcg ccagttggtt
    22561 agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag aacgataggt
    22621 cggttctgcc ggggccggga cgttatccca acctgagcgc gtagaaggcg cagcacgttg
    22681 cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg ccgcttgtgg
    22741 cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat ccgctccgca
    22801 gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc aatcgaggac
    22861 aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct gcaatgggcg
    22921 tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa gacactgctg
    22981 ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg actaaggctg
    23041 aaacattcat gattgttgac gtacgtcgaa aagaccctgc ttgagggtga cgcacgaacc
    23101 gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc gcggatcata
    23161 gcctaaactg cgcaagagat cttctgtttc tcacagattt tcccgattta tccacaggac
    23221 tttccagacc tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat ttacgccgca
    23281 tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat aaagatctag
    23341 gacgatcctt gcgctttacc catcagcccg tataatcctc cacccggcgc gccatgctgg
    23401 ttttcactgg tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa gcgcggtaaa
    23461 taaggaaaga gaattgactc cggagtgtac aattattaca atccggcctc tttaatcacc
    23521 catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc cattgaattt
    23581 attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt actgaagcgc
    23641 aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca ggttctggca
    23701 cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag ctaacccctg
    23761 agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc aattcacatt
    23821 cgtcttccag cagccacaac gggctggcac gccgcaaatt accattctcg gccgcctgaa
    23881 ttcgctgggg catccccgta tcggtcttac agtcgccaag aaaaacgttc gacgcgccca
    23941 tgaacgcaat cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc aacatgaact
    24001 cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg ataaccgtgc
    24061 tctctcggaa gcgttggaaa aattatggcg ccgccactgt cgcctggctc gcgggtcctg
    24121 atagccctca ttcgggtcta tcaacgcctg attagtccgc tactcgggcc gcattgtcgt
    24181 ttcactccaa cctgttcaag ctacggaatt gaggcattgc gcaggtttgg agtgataaaa
    24241 ggcagttggt tgacggtgaa acgcgtatta aaatgccacc ctttacaccc tggtggtgac
    24301 gatcccgtcc cgcccggacc atttgatacc agagaacact aacgatggat tcgcaacgca
    24361 atcttttagt catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc tgggagcagg
    24421 ataaaaaccc gcaacctcag gcccaacaga ccacgcagac aacgaccacc gcagcgggta
    24481 gcgccgccga ccagggcgta ccggccagtg gccaggggaa actgatctcg gttaagaccg
    24541 acgtgcttga tctgaccatc aacacccgtg gtggtgatgt tgagcaagct ctgctgcctg
    24601 cttacccgaa agagctgaac tctacccagc cgttccagct gctggaaact tcaccgcagt
    24661 ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg cccggataac ccggctaacg
    24721 gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt gctggctgaa ggtcaaaacg
    24781 aactgcaggt gccgatgacg tataccgacg cggcaggcaa cacgtttacc aaaacgtttg
    24841 tcctgaaacg tggtgattac gctgtcaacg tcaactacaa cgtgcagaac gctggcgaga
    24901 aaccgctgga aatctccacc tttggtcagt tgaagcaatc catcactctg ccaccgcatc
    24961 tcgataccgg aagcagcaac ttcgcactgc acaccttccg cggcgcggcg tactccacgc
    25021 ctgacgagaa gtacgagaaa tacaagttcg ataccattgc cgataacgaa aacctgaaca
    25081 tctcttcgaa aggtggttgg gtggcaatgc tgcaacagta tttcgcgacg gcgtggatcc
    25141 cgcataacga cggtaccaac aacttctata ccgctaatct gggtaacggc atcgccgcta
    25201 tcggctataa atctcagccg gtactggttc agcctggtca gactggcgcg atgaacagca
    25261 ccctgtgggt tggcccggaa atccaggaca aaatggcagc tgttgctccg cacctggatc
    25321 tgaccgttga ttacggttgg ttgtggttca tctctcagcc gctgttcaaa ctgctgaaat
    25381 ggatccatag ctttgtgggt aactggggct tctccattat catcatcacc tttatcgttc
    25441 gtggcatcat gtacccgctg accaaagcgc agtacacctc catggcgaag atgcgtatgc
    25501 tgcagccgaa gattcaggca atgcgtgagc gtctgggcga tgacaaacag cgtatcagcc
    25561 aggaaatgat ggcgctgtac aaagctgaga aggttaaccc gctgggcggc tgcttcccgc
    25621 tgctgatcca gatgccaatc ttcctggcgt tgtactacat gctgatgggt tccgttgaac
    25681 tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc ggcacaggac ccgtactaca
    25741 tcctgccgat cctgatgggc gtaacgatgt tcttcattca gaagatgtcg ccgaccacag
    25801 tgaccgaccc gatgcagcag aagatcatga cctttatgcc ggtcatcttc accgtgttct
    25861 tcctgtggtt cccgtcaggt ctggtgctgt actatatcgt cagcaacctg gtaaccatta
    25921 ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc cgcgagaaga
    25981 aaaaatcctg attcggtgag ttttcgctaa aataagggcg gtcagttgac cgcctttttt
    26041 cttttcgtag ggtggataag caccgcgcat ccgccacaca aagcaacagg aacatcatga
    26101 gcgataatga cactatcgta gcccaggcca cgcctccggg acgtggcggc gttggcatcc
    26161 tgcgcatctc cggcctaaaa gcccgtgaag ttgccgaaac cgtgctgggt aaactgccta
    26221 agccgcgcta cgccgattat cttccgttta aagacgccga cggcagcgtg ctcgatcagg
    26281 ggattgcgct atggttccct ggcccgaact cgttcaccgg cgaagatgtg ctggaactgc
    26341 agggtcatgg cggtccggtg atcctcgacc tgctgttaaa acgcattctg accattcccg
    26401 gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac gataaactcg
    26461 acttagccca ggccgaggcg attgccgatc ttatcgacgc cagttcggaa caggcggccc
    26521 gttcggcact taactcgctg caaggcgcat tctccgcacg ggtcaaccat ctggtggaag
    26581 ccctcaccca cttgcgcatt tacgtcgaag cggcaattga tttccctgat gaagagatcg
    26641 atttcctctc cgacggcaaa attgaagccc agcttaatga cgtgattgcc gatctcgatg
    26701 cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga agggatgaag gtggtgattg
    26761 ccggacgtcc taacgccggt aaatcgagcc tgttaaacgc gctggcgggc cgtgaagcag
    26821 caatcgtaac tgatatcgcc ggaaccacac gtgacgtgct gcgtgagcat atccacattg
    26881 acggaatgcc gctgcatatc atcgataccg ctgggctacg tgaagccagt gacgaagtgg
    26941 aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc gtgctgttta
    27001 tggtcgatgg caccacaaca gacgccgtgg atccggcaga gatctggccg gaatttatcg
    27061 cccgtctgcc agcgaaacta ccgatcaccg tggtgcgcaa taaagccgat atcaccggcg
    27121 aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg gcaaggacag
    27181 gtgaaggcgt ggaggtgctg cgtaaccatc tcaaacagag catgggcttt gacaccaaca
    27241 tggaaggcgg cttcctggcg cgtcgtcgcc acctacaggc gctggaacag gcagcggagc
    27301 atttgcagca aggtaaagcg caactgttag gtgcctgggc gggtgaactg ctggcggaag
    27361 agttgcgcct ggcacagcag aacttaagcg aaatcaccgg ggaatttacc tctgatgatt
//
LOCUS       DAJTVE010000045        27411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000045 DAJTVE010000000
VERSION     DAJTVE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(146..1585)
                     /gene="rsmF"
                     /locus_tag="OO439_001839"
     CDS             complement(146..1585)
                     /gene="rsmF"
                     /locus_tag="OO439_001839"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6316217.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1666..4299)
                     /gene="yebT"
                     /locus_tag="OO439_001840"
     CDS             complement(1666..4299)
                     /gene="yebT"
                     /locus_tag="OO439_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6316218.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4268..5551)
                     /gene="yebS"
                     /locus_tag="OO439_001841"
     CDS             complement(4268..5551)
                     /gene="yebS"
                     /locus_tag="OO439_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6316219.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5681..6178
                     /gene="msrC"
                     /locus_tag="OO439_001842"
     CDS             5681..6178
                     /gene="msrC"
                     /locus_tag="OO439_001842"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6316220.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6275..6973
                     /gene="proQ"
                     /locus_tag="OO439_001843"
     CDS             6275..6973
                     /gene="proQ"
                     /locus_tag="OO439_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6316221.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6993..9041
                     /gene="prc"
                     /locus_tag="OO439_001844"
     CDS             6993..9041
                     /gene="prc"
                     /locus_tag="OO439_001844"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6316222.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9233..10114
                     /gene="htpX"
                     /locus_tag="OO439_001845"
     CDS             9233..10114
                     /gene="htpX"
                     /locus_tag="OO439_001845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6316223.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10160..11223)
                     /locus_tag="OO439_001846"
                     /pseudo
     CDS             complement(10160..11223)
                     /locus_tag="OO439_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11400..12191
                     /gene="kdgR"
                     /locus_tag="OO439_001847"
     CDS             11400..12191
                     /gene="kdgR"
                     /locus_tag="OO439_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6316224.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12334..12573)
                     /locus_tag="OO439_001848"
     CDS             complement(12334..12573)
                     /locus_tag="OO439_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316225.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12732..12875
                     /gene="mgrB"
                     /locus_tag="OO439_001849"
     CDS             12732..12875
                     /gene="mgrB"
                     /locus_tag="OO439_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6316226.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12950..13237
                     /locus_tag="OO439_001850"
     CDS             12950..13237
                     /locus_tag="OO439_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6316227.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13907..14050
                     /locus_tag="OO439_001851"
     CDS             13907..14050
                     /locus_tag="OO439_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6316228.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14063..14272
                     /gene="cspE"
                     /locus_tag="OO439_001852"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14063..14272
                     /gene="cspE"
                     /locus_tag="OO439_001852"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6316229.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14438..15247
                     /gene="rlmA"
                     /locus_tag="OO439_001853"
     CDS             14438..15247
                     /gene="rlmA"
                     /locus_tag="OO439_001853"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6316230.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15244..15810)
                     /gene="mntP"
                     /locus_tag="OO439_001854"
     CDS             complement(15244..15810)
                     /gene="mntP"
                     /locus_tag="OO439_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6316231.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16239..16697)
                     /locus_tag="OO439_001855"
     CDS             complement(16239..16697)
                     /locus_tag="OO439_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6316232.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16752..17603)
                     /gene="manZ"
                     /locus_tag="OO439_001856"
     CDS             complement(16752..17603)
                     /gene="manZ"
                     /locus_tag="OO439_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6316233.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17616..18416)
                     /gene="manY"
                     /locus_tag="OO439_001857"
     CDS             complement(17616..18416)
                     /gene="manY"
                     /locus_tag="OO439_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6316234.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18479..19450)
                     /gene="manX"
                     /locus_tag="OO439_001858"
     CDS             complement(18479..19450)
                     /gene="manX"
                     /locus_tag="OO439_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6316235.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19913..21463
                     /gene="yoaE"
                     /locus_tag="OO439_001859"
     CDS             19913..21463
                     /gene="yoaE"
                     /locus_tag="OO439_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6316236.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21467..23065)
                     /locus_tag="OO439_001860"
     CDS             complement(21467..23065)
                     /locus_tag="OO439_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6316237.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23196..24560)
                     /gene="sdaA"
                     /locus_tag="OO439_001861"
     CDS             complement(23196..24560)
                     /gene="sdaA"
                     /locus_tag="OO439_001861"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6316238.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24744..25322)
                     /locus_tag="OO439_001862"
     CDS             complement(24744..25322)
                     /locus_tag="OO439_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6316239.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25326..26687)
                     /gene="pabB"
                     /locus_tag="OO439_001863"
     CDS             complement(25326..26687)
                     /gene="pabB"
                     /locus_tag="OO439_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6316240.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26761..26940
                     /locus_tag="OO439_001864"
     CDS             26761..26940
                     /locus_tag="OO439_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6316241.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27060..27383)
                     /locus_tag="OO439_001865"
     CDS             complement(27060..27383)
                     /locus_tag="OO439_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6316242.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7055 a         6752 c         6926 g         6678 t
ORIGIN      
        1 aagcgcctat gcgcacactg gttttcatct tcgtgctctc catttagctg ccggttgtgg
       61 tacgaccagt ccgatcgcac catttttcag cctagttgag gcagccgaat gtgccagtca
      121 aaaagtgcgc tttttttgtg cgctgtcagg cgttaccggt aaaaagtttc ccatcgcgca
      181 ccagttcacg cggatagctg tttttcaatc gcgaaccaat ccgtttggct aaaccaatcg
      241 gttgatgctg gaaagtaacc aacacgtcat ccgccactgg cgcggcttgc gggtaaacat
      301 cgcgcccgcg ataccactcc tccgcttcct gcggtgtcag ttcaaaagcg ttcacattgt
      361 cgggggtggc aagggcaata actgcttcat gctgccagcg ataacctttg ttgtgcgttt
      421 cggcaagttt aatccccaag cgagaaaatc ggactttacc gatcagggct tcaatgccca
      481 ccgggaacaa ccacagttct ttgtcacgct gccagaggcg caggttttca tcccagttta
      541 agccaacacc tgcagccgcc tgacgaattt gtccagcttc tcgatctttc actgggctga
      601 acggaaaatt accgactttg tatttggggg cgggtaaggc gggaatcgcc tgagttttac
      661 gcagacgagc aacgaagaag ccttcgcagt cgtaaatttg tgggaaaaca tgcaaaaagc
      721 cttcctcggt cagcgccttg tttgcaccag ggaagagatc accaagcggt aaaaactcta
      781 ctgcgtcggg gtaagtctct ttcagccaca ggcaaacggc ttcgttttct tcctggttta
      841 aggtacaggt cgagtaaacc agcgtaccgc cagggcgtaa tgcatgaaag gcgctgtcga
      901 tcagttcccg ttgggtagct gcgatttcct gattgctttc tggtgaccag ttttttagcg
      961 catcgggatc tttacgcacc acgccttcgc cggagcaggg agcgtccagc aaaatggcat
     1021 caaacatttc tggcactgcc gcaccaaaca cgcggccatc aaaatgtgtg agcgcaacat
     1081 tactgatacc acagcggctg atattggcat gtaacacttt tacccgactg gcggaaaact
     1141 cattggcaag aattgcccct tcgttattca tccgcgcgga aatttgcgtc gttttggagc
     1201 caggcgcggc agcgacatcc atcacccgct gtggtgcatt accgtcagca aacaaggcgg
     1261 cgacgggcaa cattgaactg gcttcctgaa tataaaacag gccacttaaa tgctcggcgg
     1321 tactacccaa tggcaatgca tcttcattgt cgcgttcaat ccagaaacct tcttcacacc
     1381 acggaattgg cgtaagcgtc cagccataag gagcggttaa ttgcaggaaa tcagcaacgg
     1441 agattttcag cgtattaacg cgaatgctgc ggcgcaacgg gcgctgacag gcggcaagaa
     1501 aatcatcaaa agagagcgtc gaaggcatcg cttcgcgcat ttgcgtcaga aaggcgtccg
     1561 ggaaataaac ggtgtgttgg gccacgagca tataccactg gcaaaaaaaa caggcgcgca
     1621 gtttagcata aacgctccgg cgcaggcacg ccggagcagt gggcattatt tgggaagcgc
     1681 agtaccccat tcacgccact ctttcggttc actttcctgc aacaggaagt gcttgccttc
     1741 ctgggctttc ggtgccagtg gcgtacccgg aggcgtggcg aaggcgatgc cgccacggat
     1801 aaactggtta aaggtgccgg ttttcactac gccgcccgtc agaccaaagt ccagactgta
     1861 acccgatgcc aaccagaaga cggaattgtt acgcaccagg tgttgatagc gtttactgat
     1921 gcgcatcgca atcatcacgc gatctgacaa tgtccccagc gtcattccgg taaccgtacc
     1981 tacttccaga ccacggaaca gcacaggcgt accaatacct aacgaaccgg cttccggtgc
     2041 ttcgacaata atgcttaagc catccaggta acgcgaatca gtaatagtag cttcttgcaa
     2101 ctcaaagtca cggcgaggat taccccgacc tggttcgaca ttgatatacg gctggaggat
     2161 agtatctaga tgctcaacgc ccgcggctga aatttgcggt gtgaccaccg agaagcgcgt
     2221 accgccgcgg gcgaaggtct ggacatactc cggatagagc accgcctttg cctgtacttc
     2281 attgcgcgcg gtaatcagat ccagcgtctg gatttgcccg atatcaatac cgagatagcg
     2341 aattggcatc ccgaccgcca gttttccggc atcgaaagcg tgaagcgtaa tctgcccacc
     2401 aaccgcacgg gccgctgttt cggaagcata cagaattcgt ttgtcgcctt tacgctgact
     2461 ggcgctggca ccgctgaggt tatcgaagct aatggctccc tttaatgctc tggagagcgg
     2521 ggatgcctgt acggtcaggc cactaccatt cagctgaact ttcgccccgc cttctgccca
     2581 gaacacgcta ttgctggtca gaaggttgcg atactccggc ttaatatgca gatcgatatc
     2641 aaacgcgtta gctcgcggac gcacggtaat cacttcacca acttcaaatt tacggtacag
     2701 cactaccgat cctgcctgca catccggcag cgtctctgca ctcaaactca cggttgtggt
     2761 gggtaaatcg ctaaggctgt tctccagcgc tttttccaga ttggcataca gtggatagct
     2821 ggctttcatc tcacctttat cgcctggcag aatacgtatc ccgccgttaa tccattctga
     2881 ggcgctggca ccaagaaact caacgccatc cagccccacc ttcacgtcga cacggctgtt
     2941 gacgacaaat ttgctatcgc cttttaccag ttcgcgatgc tgaggctcga tggcgacggt
     3001 aaaggtaacg cctttgctgg tgagtttacg atcaataacc tggcctacct gcacgccgtg
     3061 aagaatgagc ggctgaccag catcaatacc gtaactttcc ggcgcggtca gggtcagcgt
     3121 cagaacatca ggttcatgca gcagtgcttt ttcgcctggc acaacaacga actctttgcg
     3181 tggctcgcca tcgccgggta ccaactcgaa ggttttgcca gtcagcaggg cgctgagatt
     3241 ggcatcgcta agggataatt tcggattaag taattcgatg cgggtatttt cacgaagcag
     3301 ggtaacgacg ctgggattaa ctgtcatttc cccagtgact ttaccaccag gatttaaatc
     3361 cagtttagtt agctgtccga cttccagtcc ctgatacatt aatggcgtcg agtcggcggt
     3421 taatccggcc ccactcggca gttccagttt gattattacg ccacgctggc tatgggcgag
     3481 gtcttcatac agaccaaagg tatcttccgc ctcggcaggt ttcgactctt caggtgaatc
     3541 gaaggcaatc gcaccgttaa ccagtgccgc cagactttcc agtttcacct tcgcaccact
     3601 gatactgacg ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcactag
     3661 gtcggtaaaa cgccgctcga tcaggacatc aatcaccacg ccttgcttgt tgggattgat
     3721 ggcatagtcg tagacttttc ccacagggat cttgcggaaa tagaccaatg aaccgctgtt
     3781 cagcgaaccg agatcggggg cttgcaggtg gatcatcaga tcgccattgt ccagtcgata
     3841 tttcggttgg gtatcgagtg cgacaaagtg atcctgctct ttacctttac ctggcatcat
     3901 gccgatatag ttaccaccga cgagggcgtc cagcccggag acacctgcca acgatgcttt
     3961 tggcgtcacc agccagaact gagtctcttc gcgcagcgca tctttcatat cggacttgat
     4021 gctgaccttg acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc
     4081 gacttcaacg ccctgataac gaacaggcgt acggcccggg acaataccat ccgccgacat
     4141 aaagtcgatg gtgacggtat taccccggtc ctgataactg tcccaaatca gccaaccggc
     4201 aatcattagc gcgatgaaag gcagcagcca gaaaggtgag atacggcgtt tatttttaat
     4261 ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc
     4321 tgtccagcca ttccacagca agaatagtca aaattacggc tgcgccaaaa taaaacgcag
     4381 ccggtcccat agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata
     4441 tgacaaacag gtcgagcatc gaccagcgtc caatccaggt caccatccgc agtaacagaa
     4501 tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct caacagtaaa gtgaacatga
     4561 cgatcacttt agtaaacggt accagaatac tggcgataaa cacgattccg gcgaccgcaa
     4621 tgttgctact tgccagcgac ataatcccgg aaagaatcgt atcttcctgc cggccaccat
     4681 tcagataaat gatagaaata ggcaataggt tggctggtaa caacaaaacg attgacgcta
     4741 acagcgccgc ccagcatttt tgcaaactat gacgacgacg caggcgtagc gggatatggc
     4801 aacgcgggca gcgaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac
     4861 gaagtttctc gtccctacgc gtagcggggc gctgcggata atatcgctcc cacagctcct
     4921 caacattaag atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca
     4981 agccgacacc cgcctggata tgggcataat cctgtacctt tatagaagca acgccaatgc
     5041 cgaccaggta gatatccagc attacccact ctttaagacg ctcaagcatc agcagtaccg
     5101 gacgtaaatt cattcccagt cggttaccaa accataaata tgctatggag gacaccagaa
     5161 tgaggggggc accaataacg cagaaaaaga ccatcgcccc cgttatcgta tcgccctgtt
     5221 tggtcatttg ccagatgcct tgcatgacgt tggcgtcgat acgaatacct aacagccaga
     5281 tatgcaacag cggttcgccc caggcaaacg gcatcagcaa cagcatggtg aaagccattg
     5341 ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac
     5401 aataggcact ctgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
     5461 aacgttggta atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg
     5521 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
     5581 taatcttaac ccatgatggg aatgatcttg agcattaacg catttaatgc ttattttaat
     5641 aggctggcag ttccaggtaa gacaaacaag tgattatata atgaacaaaa cagaatttta
     5701 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
     5761 gcttgcgaac accagtgcgt tgttatacga gcgtctcact gacgtaaact gggcaggttt
     5821 ttatttgctt gaggacgata cactggtact cggaccattt cagggcaaaa ttgcctgtgt
     5881 ccggataccc gtcgggcgcg gggtgtgcgg cactgcggtt gcccgcaatc aagtgcagcg
     5941 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
     6001 aattgttctg ccgctggtgg tgaaaaatca gattattggt gttcttgaca tcgatagtac
     6061 cgtcttcggt cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
     6121 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
     6181 aacggataac gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc
     6241 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
     6301 cagtaaagaa gtaatcgcgt ttctggccga acgttttccc cactgtttca gtgcggaagg
     6361 tgaagcgcgt ccgctgaaaa tcggtatttt tcaggatttg gtcgatcgtg ttgccgggga
     6421 aatgaacctg agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg
     6481 ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg
     6541 tgagctggac gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
     6601 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
     6661 tgagaaagaa gacgcactgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa
     6721 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
     6781 aaaagcacct cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg
     6841 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
     6901 caaagatggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
     6961 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
     7021 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
     7081 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt
     7141 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
     7201 tctttgaccg ctacctgaat ctgctcgatt acagccacaa cgtgctgctg gcaagcgatg
     7261 ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga actgcgttca ggcaaactcg
     7321 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgagcgt taccagtacg
     7381 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
     7441 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgctgtgg gacagtaaag
     7501 tcaaattcga cgagttaagc ctgaagctgg caggaaaaac ggataaagaa attcgtgaaa
     7561 ccctgacacg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
     7621 ttttctcgct ggcaatgacg gcgtttgcgc gtgaaatcga cccgcatacc aactatcttt
     7681 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcgctggaa ggtattggcg
     7741 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggtggtccgg
     7801 cagcgaagag taaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
     7861 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggttgcctta attaaagggc
     7921 cgaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacccgca
     7981 ctgtaacatt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
     8041 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc gggcttctat gtgggtttga
     8101 cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca
     8161 tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt
     8221 ttattcctgc gggtcccatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
     8281 gcgataccga cgggcaggtg ttctataaag gcccgctggt ggtactggtt gaccgtttca
     8341 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gcgctggttg
     8401 tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata ccgttcattg aaccgtattt
     8461 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
     8521 aattctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacgcca gacatcatca
     8581 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
     8641 gggatagcat tgatgccgcg acttatgtga aatcaggcga tttaacggcc tttgggccgg
     8701 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacatcatga
     8761 aggatatcgc gcgcttcaac gctatgaggg acaagcgcaa tatcgtttct ctgaactacg
     8821 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
     8881 gctttaaacg cgaaggtaaa ccggagttga agaagctgga tgatctaccg aaagattacc
     8941 aggagccgga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
     9001 aaaaagccag acccgcggaa caacccgctc ccgtcaagta atatcaatca ggcacaagaa
     9061 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
     9121 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
     9181 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
     9241 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
     9301 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
     9361 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
     9421 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
     9481 aacccaggct cgtcaggcag ggatcgctat gccgcaagtg gctatctacc atgcgccgga
     9541 catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg
     9601 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagcca
     9661 catcgccaat ggtgatatgg tcaccatgac gctgattcag ggcgtggtga acaccttcgt
     9721 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga
     9781 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
     9841 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
     9901 ccatgctgat gccggttcgg caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
     9961 gcgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    10021 cggtaagtcg aaatcgctca gtgagttgtt catgactcac ccgccgctgg ataaacgaat
    10081 tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt
    10141 cctgatcgac gcgctttttt tatgccttgg atcgtggctg agtgatacgt aaaccactga
    10201 cacaggcagc aatcactgcc agaatagccg cagccatcag cgaaacgtgc gtaccattat
    10261 caccgaactg atttagcatc agcgccacca gtgccgcgcc gctactctga cccagtagac
    10321 gagccgttcc taacatgcca ctggctccac cgctacgttc gcgaggagcg gaggtaataa
    10381 tggtgtggtt attgggtgac tggaacaagc caaatccagc gccacataag atcatcggcc
    10441 agataatatt gatatccgca ggtgacgcgg gcagcagaac caggggaaaa agccccgcag
    10501 ccatgatgaa caaccctaga gcacccagca atcctgcatg tacgcgttca atcaaatagc
    10561 ctgccaacgg agccatcacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt
    10621 cgacttcact acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca
    10681 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    10741 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    10801 caacaacaac cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga
    10861 aaccactcaa cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    10921 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    10981 gcagggcgat aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    11041 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca atatggcgat
    11101 tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa tgccaatcac
    11161 aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg
    11221 cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt
    11281 cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt
    11341 gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa
    11401 tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg
    11461 gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg
    11521 tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg
    11581 tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg
    11641 ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct
    11701 cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca
    11761 ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc
    11821 cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatctc gatgaagtga
    11881 agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag
    11941 aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa gataacgaag
    12001 agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca
    12061 ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat
    12121 atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact
    12181 atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag
    12241 catgagaaaa ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt
    12301 actcatgagt gagaatctgg ttgtttatta cccttaacca ttatcgacca cgatattgct
    12361 tttacgtaac agtgggcaat ctgttatccc caagaaacca cttttagtgt gcaagtattg
    12421 taccgtgctg gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt
    12481 ctctttgctt ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc
    12541 tatcagcata actgtgcgaa tgatgaatcg cattacaacc tcttctcttt ttatgttcgc
    12601 ttaatcgtag cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc
    12661 gacatagtta ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag
    12721 cggagattgg agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct
    12781 tgctgctttg ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg
    12841 gaatttgtgc cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt
    12901 cttcctctcc agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt
    12961 tgtaaattca ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat
    13021 cgggctcatc ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga
    13081 actgcttgag gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg
    13141 cgaagcaaac gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga
    13201 tgacgaagat attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc
    13261 ccgtcgttga agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct
    13321 tcaaattaaa actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg
    13381 atatcagaaa aagatatttt cctcattaat ttattaggat gtttacatcg gatttgtgat
    13441 taagcgtggt attatttatt agcgaaacgt ttctctcttg atttttttgc tcattcatca
    13501 atttttctta ttttaaattt tcaatccctt tggggattga cttctcttta gggtaattga
    13561 tagccgttaa ccgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc
    13621 aaaaaatgtg atattgcacg cactatgtaa taacttctcc cactggcctg gaacaactga
    13681 acttattgaa ctatgttaga aaatacgcca gtttaagtat ctgcctgaac tggcaaggtt
    13741 aagcacaatg atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac
    13801 gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca taattaacgt
    13861 tgtatgtgat aacagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt
    13921 cagtaaagaa gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta
    13981 tattggtgcc tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa
    14041 aacaatctaa ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga
    14101 gtctaaaggt tttggcttca ttactccggc tgatggcagc aaagacgtgt tcgtacactt
    14161 ctccgctatc cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga
    14221 aattcaggac ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc
    14281 actgatctga agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct
    14341 ttttactatg ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg
    14401 ttacacttgc ggcctttagt atcctgccgg agttgtcatg tctttttcct gtccactttg
    14461 ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt ccccagcgac atcagtttga
    14521 tatggcgaaa gaagggtatg tcaatctgct gtccgttcag cataaacggt ctcgtgatcc
    14581 gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca
    14641 gccgctgcgt gatgcaattg tcgcccaact gagggaacgg cttgatgata aggccacggc
    14701 ggtgctggat attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc
    14761 cgaaatcacc acgtttggtc tggatgtttc gaaggtagcg ataaaagcgg cggcgaaacg
    14821 ctatccgcag gtcacttttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag
    14881 tatggacgcc ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt
    14941 gaagcccggc ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa
    15001 ggggctgatt tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt
    15061 tacattacag cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt
    15121 cgcattattg cagatgacgc cgtttacctg gcgtgcgaag ccagaagtct ggcaaacact
    15181 ggcagcaaaa gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc
    15241 ttattaaccg tggaagtgcg tccagaggat ctggacgccg atgccgatca gtaccagccc
    15301 gccgagaatt tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc
    15361 taatgttgac ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac
    15421 ctgcaggaaa gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt
    15481 ggttaccagt agccagaaac cgtgtcgacg gcgcggctct tcatcttcat catctgcgcc
    15541 acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca
    15601 gtggttccat tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg
    15661 cgtcagggtt tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg
    15721 tttatggagg gtggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa
    15781 cgcaagaaga acagtagcag tgatattcat gacaatgtcc tgaccggggt atccataaaa
    15841 cacatcatcg cccccagcaa acatgcatac atgaacaaca agccgtgatg gttttcgcgt
    15901 tcatgcaggc atagcgattg atgtgcatat ggtctcgcct gactgaaagt attcagtccg
    15961 tacgtgccac gttttgcaac gagtatgttg acacgtacat ttccggaatc tggaaatcgg
    16021 ctactcccca aatgagggcg caaccttaac atattttgag aatataaaac aactgttgga
    16081 atatattatt tcattgcttt attgataacg attttcattt ggatttatat atcaatgaaa
    16141 cgttaaaata aataacaaat aagggaagag gtaatatagc ctaagctatg tctgattatt
    16201 aaaaatacac taaacatagc tttggctaaa ttcataactt attgagttga aacgagaagc
    16261 ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg acgctgttct
    16321 aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg gctatattca
    16381 atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc
    16441 cagaatatat aaaaacccat cagagccagt gcgcttaata accatgtcgt tattaacgca
    16501 ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa gataacgcta
    16561 tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt acggcggggc
    16621 atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc
    16681 aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt
    16741 gtacaacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc gatgacgaag
    16801 aagccaacga tgatccacag cgggttaact tttttgcgca gtagccacat acaagcaaag
    16861 gtcagcagca gtggtaccag gcctggcatt aactggtcca gaatagtctg gacagtagta
    16921 acgtgttctt tgcccgtctg gtcagtaatg cgagagacaa ccagcgggat gttgacatgt
    16981 gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc cccttccgtc
    17041 agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt ggagtaacca
    17101 tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat gaagaacagc
    17161 agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc aaataccgga
    17221 cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat caaaccgact
    17281 ttgataccgt tgatagcacc gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc
    17341 gccagggtta cgccgagaat cggcgcagcc acgaacggct gggtgttaaa gaactccagg
    17401 tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg acgaattgcc
    17461 ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg
    17521 aagaggttag aacgcaggaa gacgccacga atatcacttt gagtgagttt tttctcggtg
    17581 gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt atcgagatcg
    17641 ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg
    17701 atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt aaagttggtg
    17761 aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc acgcatcatg
    17821 ttgataacca tcgcataacc aaccaccacg atcatgccac cggcgatatt cagaccattg
    17881 gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc aacagacagc
    17941 gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga agaaacgtgg
    18001 atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc gtgctggaaa
    18061 gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc cagagggatt
    18121 gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat ggtagaaatg
    18181 atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca gcccagcgcg
    18241 atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag aacgataccc
    18301 accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat tgatcccata
    18361 cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat ctccattgta
    18421 cttctcctgt ttacgtaaga cttaagtgtg aaaactgagt gataatcaac acaatacgtt
    18481 acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg gaaaccttac
    18541 ggacttccag ctcaataccg cgcgcattca gtttcttgaa cgcctcgata tctttttcat
    18601 caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata ccaccgacgt
    18661 taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt gggttggtaa
    18721 ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga atcattttgg
    18781 caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac
    18841 gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca ttggtttctt
    18901 tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa
    18961 taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct tttggtgctg
    19021 cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct ttcacgcctt
    19081 cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gcttgggtca tcatcacgag
    19141 ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca taatgctctt
    19201 tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta tcaacgagaa
    19261 acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtacttt tcaatcagcg
    19321 tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc tcgcctaaca
    19381 gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct ataacaatag
    19441 caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca gatgtatttt
    19501 tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa acccctgcgt
    19561 ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa
    19621 ctttcgatta gcagaaattt cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga
    19681 aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt ttaacaacca
    19741 tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc
    19801 cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat tttgtcgtta
    19861 cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg tcatggaatt
    19921 cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg aaattgtgct
    19981 gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc caaaacaacg
    20041 cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc
    20101 gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg atttctcctt
    20161 ctccggacgc gacctgatta tgttgttcgg ggggatattc ttgctgttca aagcaacaac
    20221 cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta aaggctacgc
    20281 cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct cgttggatgc
    20341 ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg cggtagtgat
    20401 tgcgatggcg gttatgttgc tggcatccaa accgctgacg cgattcgtta accagcaccc
    20461 cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc tggtggcaga
    20521 aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct tctcgatcat
    20581 catcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgccaccagt cgactttgcc
    20641 gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa
    20701 tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga
    20761 acgttacatg attaacggcg tactgacgct tgcgtcgcgt tctctgcgcg ggatcatgac
    20821 gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa tccgcgagca
    20881 actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat
    20941 cggtattgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg atgtggcggc
    21001 gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga tcaacctgtt
    21061 gggcgtgctg cgtcgtgctc gcgggagctt tgttatcgtg accaacgagt ttggtgtggt
    21121 acaaggtctg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat tcccggacgc
    21181 tgacgaaacg ccggaaatca ttactgatgg tgacggctgg ctggtaaaag gcggtacaga
    21241 tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg atgatatcgc
    21301 gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat
    21361 tgatgtaggg ccactgcata tcaccatcat tgaagccaat gattatcgtg ttgatctggt
    21421 tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcattaa cgtaacggca
    21481 taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc gccagccatt
    21541 tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa atatgcacgc
    21601 catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc accagttcaa
    21661 tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta accgcgtcag
    21721 aacctatagc tgcggtaaat gacttatcaa ttttcagcat gtcaggacgt aatgtttcaa
    21781 gccaggaaaa tgaactgttg ccggtgccga agtcatcaat cgccagtttg acgtttttac
    21841 gatgtagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt tcggtgattt
    21901 cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga tcttttatca
    21961 gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac tgactactca
    22021 tcgggaaaac atggcgctga cgaatggttt ctgccatcac atagcgggtc agtggcacaa
    22081 ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag ccctgacgcg
    22141 gattgttcca gcgcagcaaa atctctacac caatacattg ctggctgcgc gcattaagca
    22201 aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattgatt tcgcgggaaa
    22261 agctcatccg gtaagcggtc gccagccagg cgatgtagcc caccagtaaa cttagcagca
    22321 ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc cccggaccat
    22381 taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca ttgtcttcag
    22441 gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg tccaccgtca
    22501 ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc agtaagtcga
    22561 tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc cattgaatca
    22621 aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt ggttgcggtg
    22681 caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa atgctggagc
    22741 aatagagtgt tccgtcttgc accaggccaa tggatcgcac agtttggagt tttgccgcct
    22801 gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga agcaagactt
    22861 cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg tgattggcga
    22921 attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg ctcagggtcg
    22981 aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg cgataggttt
    23041 taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc gacaaacgcc
    23101 tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc gggtataaat
    23161 tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac tttgattgcc
    23221 agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccagt ttcgtacatc
    23281 gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag agccatccgt
    23341 gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg cacctgaacc
    23401 tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat ttccgccgcc
    23461 acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc agccattgaa
    23521 caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga ggcgttcatt
    23581 ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat gtccgggctg
    23641 accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc gcaggcaccg
    23701 ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac tgccagcgca
    23761 aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc gctggaaact
    23821 aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg cagtacacct
    23881 tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg cgcgaaatac
    23941 tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag accagacagc
    24001 gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa cggatacggc
    24061 acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat aaaaccgccg
    24121 ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc gtggatttgc
    24181 ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt gtcgcgcggg
    24241 aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttc tacgtcgcga
    24301 ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc aagacccata
    24361 ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc ataaacgtcc
    24421 acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc gacgaactgt
    24481 ttacctgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc caccttaaac
    24541 atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt ccagtaacga
    24601 actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta ttcacatgaa
    24661 ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca agtagtgttt
    24721 tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc cagctcacga
    24781 attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag ccatacccga
    24841 tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg taatgcctgg
    24901 gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata cggcagatcg
    24961 ggcgggataa tgccgaccac tggggttacc tgatagccag ttacgctatc gacgggcggc
    25021 agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc agcttcgcgc
    25081 agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg gaatgccact
    25141 tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc cggttgcggt
    25201 cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc ccggttaatt
    25261 tgcgggcgca aaagttgaaa gcgcgataaa aaatcatcaa gcatcaggct acggtattcc
    25321 acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt ttcctgatat
    25381 tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta tatttgtccg
    25441 ttaatggcag tcagagtgcg gatagtaatg ctggtatcca tgttgccgca aaagctcaaa
    25501 tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc gataatttcc
    25561 atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc gcgcagcaga
    25621 tcgctggcgt gtaactgttc tggtagttgt gccgttatgg tgctgaccag atgatgcacg
    25681 gcagggaagg gttccaccac gaacagctct ggtactttta ccgaacctgc tacggcaaca
    25741 cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg atctttcgct
    25801 gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag gcgtggtagc
    25861 gtgcctttaa tagggcgggt ctggatttca ctattatcac aaagaataaa ccgctctggc
    25921 gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg cgcgcggttg
    25981 gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc atgaaaacgc
    26041 tgggcgagat ttacctgata gcaatcaccg ctgtgcagat attcctgtac ctggcgaaat
    26101 ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtaag cgtgaaatct
    26161 tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac atcattatga
    26221 ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc gtagataccc
    26281 actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga ctcaaaacgg
    26341 cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa atcttcgtta
    26401 tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag cgggtcatca
    26461 gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa agtggttaaa
    26521 gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc ggcatagccg
    26581 gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa ttcagcggcg
    26641 tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag tcctgactct
    26701 actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca ggagttaaca
    26761 atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt cgagcggatc
    26821 caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgttggt ggcagaagag
    26881 ctgcgtgcca accattccgg cgagcgaatc gtggcgcgtt ttgaggatga agacgagtaa
    26941 cacactaatc cgtattgatt tcgttataca agccgatgat tatttgattg tgagaaaaag
    27001 gacgcttttt atatgtgatt aaaagcgtcc tgatccattt atgctgatgt aaattttact
    27061 cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg atttttaatc
    27121 acagagcgtt cacctgactc catttttttt gcccatccaa ccattttttc cgtaaaccca
    27181 gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc aggtactccc
    27241 agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga acttggtgtt
    27301 ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac agcaggcata
    27361 ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt a
//
LOCUS       DAJTVE010000046        27368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000046 DAJTVE010000000
VERSION     DAJTVE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..216
                     /locus_tag="OO439_001866"
     CDS             <1..216
                     /locus_tag="OO439_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6316243.1"
                     /translation="DPRLDEVSDKQWYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIV
                     DGVAWKVRIDAGVAALDYRGMVRAISS"
     gene            475..756
                     /locus_tag="OO439_001867"
     CDS             475..756
                     /locus_tag="OO439_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316244.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            1396..1716
                     /gene="clpS"
                     /locus_tag="OO439_001868"
     CDS             1396..1716
                     /gene="clpS"
                     /locus_tag="OO439_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6316245.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            1747..4023
                     /gene="clpA"
                     /locus_tag="OO439_001869"
     CDS             1747..4023
                     /gene="clpA"
                     /locus_tag="OO439_001869"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6316246.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4429..4516)
                     /locus_tag="OO439_001870"
     tRNA            complement(4429..4516)
                     /locus_tag="OO439_001870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4480..4482),aa:Ser,seq:gga)
     gene            complement(4770..4988)
                     /gene="infA"
                     /locus_tag="OO439_001871"
     CDS             complement(4770..4988)
                     /gene="infA"
                     /locus_tag="OO439_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6316247.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5273..5977)
                     /gene="aat"
                     /locus_tag="OO439_001872"
     CDS             complement(5273..5977)
                     /gene="aat"
                     /locus_tag="OO439_001872"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6316248.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(6019..7740)
                     /gene="cydC"
                     /locus_tag="OO439_001873"
     CDS             complement(6019..7740)
                     /gene="cydC"
                     /locus_tag="OO439_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6316249.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(7741..9507)
                     /gene="cydD"
                     /locus_tag="OO439_001874"
     CDS             complement(7741..9507)
                     /gene="cydD"
                     /locus_tag="OO439_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6316250.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(9630..10595)
                     /gene="trxB"
                     /locus_tag="OO439_001875"
     CDS             complement(9630..10595)
                     /gene="trxB"
                     /locus_tag="OO439_001875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6316251.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            11140..11634
                     /gene="lrp"
                     /locus_tag="OO439_001876"
     CDS             11140..11634
                     /gene="lrp"
                     /locus_tag="OO439_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6316252.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            11769..15836
                     /gene="ftsK"
                     /locus_tag="OO439_001877"
     CDS             11769..15836
                     /gene="ftsK"
                     /locus_tag="OO439_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6316253.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15991..16602
                     /gene="lolA"
                     /locus_tag="OO439_001878"
     CDS             15991..16602
                     /gene="lolA"
                     /locus_tag="OO439_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6316254.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            16613..17956
                     /gene="rarA"
                     /locus_tag="OO439_001879"
                     /gene_synonym="mgsA"
     CDS             16613..17956
                     /gene="rarA"
                     /locus_tag="OO439_001879"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6316255.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            18047..19339
                     /gene="serS"
                     /locus_tag="OO439_001880"
     CDS             18047..19339
                     /gene="serS"
                     /locus_tag="OO439_001880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6316256.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            19578..22022
                     /gene="dmsA"
                     /locus_tag="OO439_001881"
     CDS             19578..22022
                     /gene="dmsA"
                     /locus_tag="OO439_001881"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6316257.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            22033..22650
                     /gene="dmsB"
                     /locus_tag="OO439_001882"
     CDS             22033..22650
                     /gene="dmsB"
                     /locus_tag="OO439_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6316258.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            22652..23515
                     /gene="dmsC"
                     /locus_tag="OO439_001883"
     CDS             22652..23515
                     /gene="dmsC"
                     /locus_tag="OO439_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6316259.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(23551..24177)
                     /locus_tag="OO439_001884"
     CDS             complement(23551..24177)
                     /locus_tag="OO439_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6316260.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            24492..25640
                     /locus_tag="OO439_001885"
     CDS             24492..25640
                     /locus_tag="OO439_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6316261.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            25850..27280
                     /locus_tag="OO439_001886"
                     /pseudo
     CDS             25850..27280
                     /locus_tag="OO439_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(27281..>27368)
                     /locus_tag="OO439_001887"
     CDS             complement(27281..>27368)
                     /locus_tag="OO439_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6316262.1"
                     /translation="QYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         6771 a         6673 c         7320 g         6604 t
ORIGIN      
        1 gatccccgcc ttgatgaagt cagtgataag caatggtaca tgctttcagc acagggaacg
       61 gacacaattg aggtggctta tcttgacggt aatgacgagc cttaccttga gcagcaggag
      121 ggctttattg ttgacggtgt ggcctggaag gtccgcattg atgctggcgt tgctgctctg
      181 gattatcgcg gaatggtcag agcgatttct tcatgagaac aaggcggcag cagccgcctt
      241 ttttgcgggt cctcctggtg gggggggcct gtccacgggg cgggtcggcg cggaaaaaag
      301 cgcatttttg tggttttatc gtcatcatca tcatgtgtgt aacctgttgt ttttaatgta
      361 gttgatgcaa aaaagatgat gattgtggtt aatttttgtt cgacatcttt tagcgtgaca
      421 gattctttac aaaaaatctg agcttgtttt cttaaccagc gcgatggggg cacaatgaca
      481 gaagccgaaa tactgggatt aatccgccgc gtcgccggaa tcagccagca ggttgacgaa
      541 caggccatgc aaccggacag cgtgaccgct gaaaattatg cgcgtgtggt ggatgaggta
      601 atgcgccgtg acggtattga gcttaacggc gtggatatgc gcaacatacg aaccagagtt
      661 cttgagttgc tggcataccg tcgccgttct caacaacgga gggagagcgc gaaaaatact
      721 taccagtgga ggaagccgga gcacttacgg cggtaactgg ttgatattac cgaaagtgca
      781 aaaatgcact tagcaactgg tggcgagttg cagatctgca actcgactat gaaactacgg
      841 aaactacccg tagtttgggt agtaagagta acacccagat tttggggctt actcgcgata
      901 cccaaataaa gggtatcggt ggaagaaata tcgtttctca tatgtgagta ccgagggcgg
      961 aattccgcct tcggttactt attgtgcgca tgcacaggga ggggcgggtc aaatctctgt
     1021 accctgacgt cttccggact gccagcccca tcgatttttt atacccgcga aaaatgaaaa
     1081 acgcttcacg ctggtgggcc tgatgccgat aactggtttc atgactgata gccggaaaat
     1141 gataacgatt aaagcgtgtt gatctttgtt cgtggttgtt cgatattgtt cgtggaacgg
     1201 tgtagttaat ggtgtagtta attttactgt ttttgacgag cttattgttt gcaactcaat
     1261 gaaaaataaa ggctaataag ccaaatgcta taaatgatag ttatctatca tgtggagtag
     1321 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
     1381 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
     1441 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
     1501 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
     1561 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
     1621 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
     1681 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
     1741 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
     1801 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
     1861 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
     1921 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
     1981 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
     2041 ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
     2101 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaattttatc
     2161 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
     2221 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
     2281 cttgcgcgcg tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
     2341 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
     2401 ggtaaaaccg cgattgcgga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa
     2461 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcacaaaa
     2521 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
     2581 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggggc agcgtctggt
     2641 ggtcaggttg atgcggctaa cctaatcaaa ccgttgctct ccagcggtaa aattcgtgta
     2701 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
     2761 cgtcgcttcc agaaaattga tattactgaa ccgtcaatcg aagaaactgt tcaaatcatc
     2821 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
     2881 cgtgcggcgg tagagctggc ggtgaaatac atcaatgatc gtcatctgcc ggataaagcg
     2941 attgatgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
     3001 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcacg cattccagag
     3061 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
     3121 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
     3181 gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc tgtttgccgg tcctaccggg
     3241 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gcttctgcgc
     3301 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
     3361 ggatacgttg gttttgatca gggcggtttg ctgaccgatg cggtaatcaa gcatccacat
     3421 gcggtgctgc tgctggacga aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg
     3481 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
     3541 gtggtgctgg tgatgaccac caacgccggg gtacgggaaa ctgagcgcaa atccattggt
     3601 cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat ctttacaccg
     3661 gaattccgta accgtctcga caacattatc tggtttgatc atctgtcaac cgacgtgatc
     3721 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
     3781 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
     3841 atgggcgctc gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
     3901 gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag
     3961 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
     4021 taatctaatt gttaggtagg ttggtgaagt ccgtaatctc gaaagaggtt acggactttt
     4081 tgtttatggg gtggaacggt gaaaacccta tttttggagg tgaaggtaat ttgttgataa
     4141 ttagtgctgc tggaaaaggt aaggataaaa aagggtgaca gcaggaaaat gagatggttt
     4201 tgctttatta acaacggact aaacgtgtag tatttgagtt cactgccgta caggcagctt
     4261 agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta caggcagctt
     4321 agaaattcac aggtaacata ctccacccgc ccaccatgtt cactgccgta cagaaagata
     4381 aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg
     4441 ggggattcga acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc
     4501 actcggacac ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa cggggcgcta
     4561 ctatagggag ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca
     4621 tactgtaaac aagttgtgca gtatatctac atcgagacaa gttacggact tatacttcca
     4681 aagtacttca tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct
     4741 ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg
     4801 ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag
     4861 tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg
     4921 cggaacatgg tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct
     4981 ttggccatct aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga
     5041 agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta
     5101 ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata
     5161 aacgatgggc gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcataac
     5221 caactctgcg accgcaatta taacactctg gggagaaatg tgccgaaaac attcattttt
     5281 gtggtgaaaa caagcatcgt ggcacccaga aattattcgg caatcgtccg aggcgcattt
     5341 gattgagata attcagataa tcccgacggg gaatttcgca ggcaccaagc gatgctgtgt
     5401 gatcgttaag gacctggcag tcgataagct taccgccatg actgataaat tcctcacaga
     5461 ataccagaag cgccgtttta gacgcatttt ccatccggct gaacatggac tcgccacaaa
     5521 atagcgttcc ctgggccacg ccgtacatac cgccgacaag ctcatcttca tgccagactt
     5581 caatggagtg ggcatgaccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg
     5641 tgatccaggt tccttcttcg cgatcgctgg cacaaccttc aatgacctga ccaaaagcgt
     5701 aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat
     5761 gcagtgattc aggccaaagc accgcgcgag gatcgggcga ccaccagagg atgggggcgc
     5821 ctggagaaaa ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa
     5881 gatcgccccc cagagccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga
     5941 aggctattga atggcgagaa agctgaacca ggcgcatgac cgcaaaactc cacgcaagtc
     6001 ggatcgttca ataatagctt acaaaccctg cttaaactgg taataacgcc cctgtctggc
     6061 aagcagttct gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg
     6121 ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat
     6181 catttctgca agcaattcaa ggatctggct ttcggttgtg gcatctaagc cttcggtagg
     6241 ttcatccagc aacaccagtg gcgcatcatg taacagcgca cgggcgatag ccagacggcg
     6301 cagttcacca ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcgtc
     6361 ctcgagcagc ttctccagac caacgcgacg caagatctcc gacagagcct catcactact
     6421 gccaggcgag gcgagtaaca gattatcacg cagcgtggcg ctaaacagat gcactcgctg
     6481 aggaacaacg ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc
     6541 gttaagcaaa atctcgccct gttgcgggtc ccaagcgcgg gtcagcagtt gtaacagtgt
     6601 tgatttgccg catccggttc gcccgagaat cgcaatatgt tccccggcgt ttacctgaag
     6661 agaaatccct ttaagtgcct gttgggattg ctccggataa gtgaactgaa catcccgtaa
     6721 tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca gggaaggtga cttccggttt
     6781 ttgatccgtt aaatcagtga tccgaacggc agaggcaatg acttgcccca gatgctgaaa
     6841 tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac gcgcagaaga caaacagggc
     6901 aattaacgcg ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag
     6961 gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga
     7021 ttgacggcgt tgcgcttcca gccattgaat ttctgtattc tctagttgcg tgcgataacg
     7081 atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg
     7141 gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa
     7201 cagcggtggc atcaggaaaa gcgtcagtaa cataatgccg cccagcgtaa aggcgagggt
     7261 gaaatcaagg aaacttaacc cgactgtcac caccataatc accacaaaag cgcccaccag
     7321 cggcgagata acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcgatt
     7381 gagcaattcg ccctgacgat agcgcgccag tccggcaggg gagaggggca acaatttgct
     7441 gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg
     7501 ttcaaaataa cgcccggcag tacgggtgat tgcggcacca cgcacgcccg cagcgggtag
     7561 catatagttg aagctgtaca gtccggcacc ccccgcaacc gctgaggccg agaggaacca
     7621 gccggaaagt gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc
     7681 aagacttaac atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat
     7741 ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa
     7801 ttccgcgtaa cgtccttgct caataatctg accatcctgc attacccaaa tgacatccca
     7861 gtcagcaaga tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc
     7921 attcagcgcc tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc
     7981 caacagtaat agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg
     8041 ccccacggaa aggcgggcag cctggtcgcc aacaggcgta tcaacgcctt gtggcaggag
     8101 cggtagaaac tcgctgaccc aggcgttatc cagcgctgct tgtaattcat gttcgctggc
     8161 atcaggtcgc gccagtagta cgttatcccg taatgttgct gccggtaatt gtggattttg
     8221 cccaacccag gagagatgtt tacgccatga ttctggtgat aaatcgcgta attctatccc
     8281 gttgattcgt agcgatccct gatatgagag aaaaccagaa agcgcgttca acagtgaact
     8341 tttacctgaa ccgctgcgac caaccaacac ggcacgttgg cctgctggca aagtaaagtt
     8401 cagcggtccg gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat
     8461 ggtcaccgga tcggtcgatg ctaattccac ctcgccacgc tgcggatggg cgagcggggt
     8521 ttccataaac gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa
     8581 cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc
     8641 cgcagccagc gtcacgccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa
     8701 accaaagtag accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga
     8761 ggataaaaat gccagccgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga
     8821 acgaatactt tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg
     8881 caggcgatcg aggaaatgcc cacttaagcg agcaagagcg agaaagttac gtcggttagc
     8941 atcggcagcc cccattccaa ccagcgccat aaacaacgga attaacggtg cagtgcccag
     9001 cagaatgagc gccgcagccc agtttgaggg gaagattgcc accacaatca gcaacggcac
     9061 cgacactgcc agcgccattt gcggcaggta gcgtgcatag taatcatgca tatcgtcaat
     9121 ttgctcgagt accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc
     9181 tgcttgttgc agacggtcga gaacctgacg gcggatggca aagcggatat gctgcccggc
     9241 gtgataaccc acccgttcgc gtaaccagac cgcccatgcg cgcagtacaa aggtcagaac
     9301 cagtaacgta aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg
     9361 cagaattcgc gccatgaacc aggcctgggc aatgatcaat atgccgctta caaagcccag
     9421 cagacgagaa atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt
     9481 taactctttt tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt
     9541 tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc catagtcgca aggtgtcgcc
     9601 ttctttactt ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag
     9661 cgttccgcat caagtgctgc catgcagcca gtaccggccg aagtaatggc ctggcgataa
     9721 atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta
     9781 ccatgaatac ccgactgtac tttgatgtag ccgttttcca gttccagctg cccttcgaaa
     9841 atcgcagtat tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg
     9901 atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg
     9961 gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc cactttatcc
    10021 atcagacgct taataaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc
    10081 acttccgaag cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg
    10141 atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc
    10201 ccacggcctt taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg
    10261 gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg
    10321 ttttgcagat ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca
    10381 tgttcgtgca tgcgctccat taataacgga ccggtcagat cgtttggatc accaggccag
    10441 ttttccactt ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca
    10501 ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc
    10561 aggataagca gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac
    10621 aatgggcagg attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac
    10681 aatttgtgca atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg
    10741 ctacttaaat ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga
    10801 atatctggca tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc
    10861 gaaaacattc gaggaagaaa aaaaacagta ttcttatatg cgcataacca tgcatgtaaa
    10921 taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg
    10981 tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga
    11041 atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat gtttcagggg
    11101 cagacaggag tagggaagga atacagagag acaataataa tggtagatag caagaagcgc
    11161 cttggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg
    11221 cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag
    11281 cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct gcttaacccc
    11341 cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg
    11401 gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat
    11461 ttagtatctg gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtcagcttac
    11521 cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac
    11581 gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga
    11641 acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag caacagtact
    11701 ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag
    11761 agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta
    11821 gcggccgccg ccttctggag gcgttgctga tccttattgt cctgtttgcc gtctggttga
    11881 tggctgcctt actaagcttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg
    11941 aacctatcca taatttaggt gggatgcccg gtgcgtggtt ggcagatacg ctgttcttta
    12001 tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct
    12061 ggcgtcatca gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg
    12121 gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct
    12181 ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc
    12241 tacacagtag cgggggaact attgcgctgc tctgcgtttg ggcagcgggc ctgacgttgt
    12301 tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaacattc
    12361 tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg
    12421 aagacgacga agagtatgaa gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc
    12481 gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc attaatccga
    12541 tggggcggca aacagacgct gcgttgttct ccggtaagcg gatggatgat gacgaagaga
    12601 ttacctacac tgcacgcggt gtggctgccg accctgacga cgtcctattt tcgggcaatc
    12661 gtgcaacgca gccagaatat gacgaatacg atccattatt aaacggtgcg ccaattaccg
    12721 aacctgtcgc tgtagcagct gctgctacca cggcggcaca aagctgggct gcgccggttg
    12781 aacctgtgac tcagacgccg cctgttgcct ctgttgatgt tccaccttcg caacctacag
    12841 tagcctggca gcctgtaccg ggtccacaaa cgggagagcc ggttattgct cctgcaccgg
    12901 aaggttaccc acagcagcca caatatgcgc agcctgcagt gcaatataat gagccgctgc
    12961 aacaaccagt acagccacag cagccgtatt atgcacctgc agctgaacaa cctgtgcaac
    13021 aaccttacta cgctcctgcg gcggaacaac ctgttcagca accgtattac gcccctgcgc
    13081 cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc
    13141 cacagtctac ataccagact gagcaaactt atcagcaacc agccgctcag gagccgttgt
    13201 accaacagcc gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag
    13261 agacaaaacc cgcgcgtccg ccactttact attttgaaga agtggaagag aagcgagccc
    13321 gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag
    13381 aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaaaccg
    13441 ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg
    13501 ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg
    13561 tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgcc
    13621 gtgaattggc gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc
    13681 gtgaagccca gcgcaatcag tacgattctg gcgatcagta caacgatgat gaaatcgatg
    13741 cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg
    13801 aacagtatca acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg
    13861 aactggctcg tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg
    13921 gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg
    13981 atggtccaca cgaaccgttg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac
    14041 cggttgcacc gcagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcaat
    14101 atcagcagcc gcaacagccg gttgcgccgc agccacaata tcagcagccg caacaaccgg
    14161 ttgcgccaca gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc
    14221 aacagccgca acaaccggtt gcgccgcagc agcagtatca gcagccacaa cagccagttg
    14281 cgccacaacc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc
    14341 cgttgcataa accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg
    14401 aagtggagcc ggtagatacc tttgcgcttg aacaaatggc tcgcctggtg gaagcgcgtc
    14461 tggctgattt ccgtattaaa gccgatgttg tcaattactc tccggggccg gttatcactc
    14521 gctttgaatt gaacctggca ccgggcgtaa aagcggcgcg catttctaac ttgtcacggg
    14581 accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac
    14641 cctatgtagg tctggagtta ccgaataaaa aacgacaaac cgtttatctg cgcgaagttt
    14701 tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata
    14761 tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga
    14821 ctaccggttc cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag
    14881 cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt
    14941 atgaaggcat tccgcatctg ttaacggaag tcgttactga tatgaaagat gccgccaacg
    15001 cgctgcgctg gtgtgttaac gagatggagc gtcgttataa actgatgtct gcgctgggtg
    15061 tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga
    15121 ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa
    15181 aagaaccata cattgtggtg ttggttgacg aatttgccga cctgatgatg acggtaggta
    15241 aaaaagtgga agagctgata gcacgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc
    15301 tcgtactggc aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata
    15361 ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc
    15421 aggctggcgc ggaatcactg ctggggatgg gggatatgct ctactctggg ccgaactcca
    15481 cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc
    15541 aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacttcc gacagtgaaa
    15601 gcgaaggtgg cgcgggcggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg
    15661 cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc
    15721 gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca
    15781 gcgaacaggg gcacaacggt aatcgtgaag tgctggctcc accgccgttt gactaattaa
    15841 tgcattgccg gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag
    15901 tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg
    15961 atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta
    16021 ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc
    16081 ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc
    16141 gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg
    16201 catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat
    16261 aacccgttcg ttgagcaagc tacggcaact tggctgaaag atgccaccgg taatacgccg
    16321 tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat
    16381 ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt
    16441 aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc
    16501 agcagttatc aactgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc
    16561 accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa
    16621 tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga
    16681 aaatttagca cagtatatcg gccagcaaca tttgctggct gcggggaagc cgttgccgcg
    16741 cgctatcgaa gccgggcatc tgcattctat gatcctctgg gggccaccgg gtaccggcaa
    16801 aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc
    16861 cgtcacctct ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa
    16921 tgcaggtcgc cgcactattc tttttgttga cgaagtgcac cgattcaaca aaagccagca
    16981 ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga
    17041 aaacccgtcg tttgagctta attcggcact gctttcccgt gcccgtgtct atctgttgaa
    17101 atccctgggt acagaggata ttgagcaagt actaactcag gcgatggaag acaagacccg
    17161 tggttatggt ggtcaggata ttgttctgcc agatgaaaca cgacgcgcca ttgctgaact
    17221 ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga
    17281 agtcaatgat agcggtaagc gggtcctgaa gcctgaatta ttgaccgaaa tcgccggtga
    17341 gcgaagcgcc cgctttgata acaaaggcga tcgcttttac gatctcattt ccgcactgca
    17401 taagtcggta cgtggtagcg cacccgatgc ggcgctctac tggtatgcgc gaattattac
    17461 cgctggtggc gatccgttat atgtcgcgcg tcgttgcctg gcaattgctt ccgaagatgt
    17521 cggcaatgcc gacccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg
    17581 cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc
    17641 gccaaaaagc aacgctgtct acactgcgtt taaagctgcg ctggcggatg ctcgcgaacg
    17701 cccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat
    17761 gggctacggg caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga
    17821 ggtttacttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct
    17881 tgaaggcaag attggcgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat
    17941 aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca
    18001 ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa
    18061 tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct
    18121 ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga
    18181 aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg
    18241 ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc
    18301 aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa
    18361 cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg
    18421 ctggggtacc ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat
    18481 gcactctggc ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat
    18541 gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac
    18601 cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta
    18661 cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga
    18721 agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct
    18781 ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc
    18841 atgcttccgt tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca
    18901 ccagttcgac aaagttgaaa tggtgcagat cgtgcgcccg gaagactcaa tggcggcgct
    18961 ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa
    19021 aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt
    19081 atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt
    19141 ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt
    19201 tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa
    19261 ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg
    19321 actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct gcgggcgctt
    19381 tttttgtctc cctttgatac cgaacaataa ttactcttca cttacacgta atactacttt
    19441 cgagtgaaaa tctacctatc tctttgattt tcaaattatt cgatgtatac aagcctatat
    19501 agcgaactgc tatagaaata attacacaat acggtttgtt actggaatca atcgtgagca
    19561 agcttgagtg agccattatg aaaacgaaaa tccctgatgc ggtattggct gctgaggtga
    19621 gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct ggcaatggcc agcagcgcat
    19681 taacattacc ttttagtcgg attgcgcacg ctgtcgatag cgccattcca acaaaatcag
    19741 acgaaaaggt tatctggagc gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta
    19801 tgcacgtcgt ggacggtgaa atcaaatatg tcgaaacgga caataccggc gatgacaatt
    19861 acgacggcct gcaccaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt cgccgtgtct
    19921 acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat
    19981 tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga
    20041 tcaaagagta cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca
    20101 ccatgacccg ctcctggccg ccgggaaata ccctggtcgc gcggctgatg aactgctgcg
    20161 gcggctatct gaaccattac ggcgactact cctccgcgca aattgcggaa ggtttgaact
    20221 atacctacgg cggctgggca gatggcaaca gcccgtcgga tatcgaaaac agtaagctgg
    20281 tagtgctgtt tggtaataac cctggcgaaa cgcgaatgag tggcggtggg gtgacttact
    20341 atcttgaaca ggcacgccag aaatctaatg cccgcatgat catcatcgat ccgcgctata
    20401 ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccggga acagatgccg
    20461 cactggttaa cggtctggcg tacgtcatga tcactgaaaa cctggtggat caggcattcc
    20521 tcgataaata ttgcgttggc tacgatgaga aaaccctgcc agccagtgcg ccgaaaaatg
    20581 gccactataa agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat
    20641 gggcctcaca aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg
    20701 gcagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg
    20761 aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg
    20821 gagggaacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct
    20881 tggaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg
    20941 gcccggaaat gacggcgctg cgtgatggtg tacgcgggaa agataagctg gatgtgccga
    21001 tcaaaatgat ctggaactat gccggtaact gcctggttaa ccagcattct gaaatcaacc
    21061 gtacccatga aatccttcag gatgataaga agtgcgagct gattgtggtt atcgactgcc
    21121 acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac
    21181 agatggactt tgcgctggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc
    21241 aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa
    21301 aacgtcttgg cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc
    21361 atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca
    21421 agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc
    21481 gtgaagatcc gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc
    21541 aggcgctggc tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac
    21601 tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc
    21661 tgcagcttac gggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg
    21721 tgctgaaagc agcttgccgt caggaaatgt ggatcaaccc gcttgatgcg caaaaacgcg
    21781 gtatcaacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaagtt catattgagg
    21841 cgaaagtgac gccacgaatg atgccaggtg tggtcgcact gggtgaaggt gcctggtatg
    21901 acccggatgc aaaacgtgtc gataagggtg gttgtattaa cgtactgacc actcaacgtc
    21961 cgtctcctct cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt
    22021 aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt
    22081 tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagcttc
    22141 cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag
    22201 aacgtgtttg cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact
    22261 aaagtctgcc cgagcggtgc gatgcataaa cgtgaagatg gttttgttgt ggtcgatgaa
    22321 gatgtgtgca ttggctgccg ctactgccat atggcttgcc cgtatggcgc accgcaatat
    22381 aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt
    22441 aaaaagccga tctgtgttga atcttgtccg ctgcgggcgc tggatttcgg ccctatcgac
    22501 gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt
    22561 accaaaccga atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact
    22621 ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct
    22681 gatgatcttc acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc
    22741 gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg
    22801 cttatgggtg ctgatgggca ttggctttat cgcttctatg ctccatcttg gttcaccgat
    22861 gcgcgctttt aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag
    22921 cggttcgatc ttttttgccg taggcggcat cggctggttg ctggcaatgc tgaaaaagct
    22981 gtcaccggca ttgcgtacgc tttggctggt ggtgacgatg gttcttggcg tcgtctttgt
    23041 ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg
    23101 gacgccgatg ggcttcttcc tgacgatgtt catgggcggc ccgttactgg ggtacctgtt
    23161 gctgagtctg gctggcgtcg atagctgggc gatgcgtctg ctgccagcca tttccgtact
    23221 ggcactggtg gtgagtggcg tggtgtcagt gatgcagggc gcagagctgg cgactattca
    23281 tagttctgtg cagcaggccg cagcgctggt gccggactat ggtgcgctga tgtcctggcg
    23341 gatcgtgctt ttggccgttg ccctgtgctt gtggattgca ccacagctaa aaggttatca
    23401 gcctgcggtt ccgctactat ccgtctcatt cattctgctt ctggcagggg aattaatcgg
    23461 tcgcggcgta ttctatggtt tgcatatgac cgtggggatg gccgtcgcaa gctaatcagt
    23521 aacaaccggg gtttcggccc cggttctctt ttatttctgc ttcgttaacg tgtcataact
    23581 ggtcatcaaa ttacgataat caggaatatg gttagagaac aacgtcgcca gtccttcaat
    23641 atcattacgc cagtcacggt gcagctcgca agccacgccg aaccatgtca tcaattgcgc
    23701 acctgcttgc gacatgcgat cccatgccga atgccgggta atttcattaa aggtccctga
    23761 cgcgtcagtc acaacaaaca catcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc
    23821 cacgcaaact tcggttacca caccggcaat aattaactgt tttttacctg tcgctttgac
    23881 tgcttttaca aaatcttcgt tatcccaggc gttaatattg cccgggcgag caatgtaagg
    23941 tgcatcgggg aattgtgctt tcagttctgg aactaatggg ccgttaggac cggtttcaaa
    24001 actggtggta agaatagttg gtaagttgaa atacttagcc aggtcaccca gcgccagcac
    24061 gttattttta aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg
    24121 atcaacaagc aaaacagcag catcattttt atcaagacga acatacggtt tggtcatcct
    24181 cttctccttt cgagagtcag tggtacgcct gaaatcggtc agacgtgatg cattgctctg
    24241 aaagcataga cgggaaatat gagtttgctg tgaccatgaa atttttcgac tgaacgaagc
    24301 gtatgaaatt tgtgttagat caataaaaac aatcagatga gattaatcat ctcttcagta
    24361 ttagcgttat gaatgagtaa tataaatcga taataccgcc gttgcgatat gcgcgtattt
    24421 tcacccattg acaatgtttt tggcggtggc atgatgcgca tgaaatttga acttcctcac
    24481 ggttttaatt catgtccacg tatacccggc ctgtcatgct tttgctgtct ggcctgcttt
    24541 tgttgactct ggcgatcgcg gtgttaaata cactcgtgcc gctttggctc gcccaggaac
    24601 acatgtccac atggcaggta ggcgttgtca gctcatccta ttttaccggc aaccttgtcg
    24661 gtacattgct gacagggtat gtcattaaac gcattggttt taaccgcagc tattatctgg
    24721 cctccttcat ttttgccgct ggctgtgccg gccttggcct gatgattgga ttctggagtt
    24781 ggttggcgtg gcgttttgtc gcgggcgtcg gctgtgccat gatttgggta gtcgttgaga
    24841 gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg tttgcttgct gcatacatga
    24901 tggtttatta cgtgggaacg tttttaggcc agttactggt cagcaaggtc tcaaccgagc
    24961 tgatgagcgt attgccgtgg gttacaggtt tgacattggc agggatctta ccgctgttgt
    25021 ttacgcgtgt cctgaatcag caggctgaaa accatgattc gacgtcaatt acggcaatgc
    25081 taaaactccg tcaggcgcgg cttggcgtga atggctgcat tatctcagga atcgttctgg
    25141 gatctctata tggcctgatg cctctgtacc tcaatcacaa aggggtgagc aatgccagta
    25201 ttggtttctg gatggcggta ctggtcagtg cgggtatcct cggacaatgg ccgattggac
    25261 gtctggcgga taagtttggt cgactgctgg ttttgcgtgt tcaggtcttt gtcgtcattc
    25321 tcggcagtat tgcgatgctt agccaggcgg cgatggcccc agcgttattc atcctcggtg
    25381 ccgctggctt tacgctatat ccggtggcga tggcatgggc ttgcgagaaa gttgaacatc
    25441 atcaactggt ggcgatgaac caagccttac tgttgagcta tactgtggga agtctgcttg
    25501 gcccgtcatt taccgctatg ctaatgcaga atttctccga taatttattg tttatcatga
    25561 tcgccagcgt atcgtttatc tatttgctga tgctgctgcg caacgccggt catacgccga
    25621 aacccgttgc tcacgtgtaa atgaattcaa gcagagtgtg aacttactgt ttcacactct
    25681 gcttttttgt ttcttctatc tgacttgctt tattccaaat tttattcgtt taaaaataaa
    25741 atgtgtagca ggttataatt ttgcatttcg ctatttccgc acttcttatt tgccgcgcat
    25801 aatccctcgt tttacagatg cccctataat tttggcgaag gatttgtcta tggctgggaa
    25861 tgttcaggaa aaacagctgc gatggtacaa cattgcgctg atgtctttta tcactgtctg
    25921 gggttttggc aacgttgtta ataactacgc caaccagggg ctggtggttg ttttttcatg
    25981 ggtgtttatc tttgcgctct atttcacacc ttatgcgcta attgttggtc agttaggctc
    26041 gaccttcaaa gatgggaagg gcggggtcag tacctggatt aaacacacga tgggacccgg
    26101 actggcttat ctcgccgcgt ggacctactg ggtggtgcat attccctatc tggcacaaaa
    26161 accctaggca attctgattg cgctcggttg ggcgatgaaa ggcgacggtt cgctaatcaa
    26221 agaatattca gtcgtagcgt tacaggggtt aacgctggtg ctgtttatct tctttatgtg
    26281 ggttgcttca cgcggtatga aatcgctgaa aatcgtcggt tctgtggcag ggattgctat
    26341 gtttgttatg tcgctcctgt atgtggcgat ggcggtaacc gcgcctgcaa ttactgaagt
    26401 gcatattgcg accacaaaca ttacctggga aacgttcatt cctcatatcg actttaccta
    26461 cattaccact atttcaatgc tggttttcgc ggttggcgga gcagagaaga tttctcctta
    26521 cgttaatcaa acgcgcaacc caggaaaaga atttccaaaa gggatgttat gcctggcggt
    26581 gatggttgcg gtttgtgcca ttctgggctc gctggcgatg gggatgatgt ttgattcgcg
    26641 taatatcccg gatgacttaa tgaccaacgg tcagtattac gcctttcaga agctgggcga
    26701 gtattacaac atgggtaata ctttaatggt gatttacgcc attgcgaata ccctgggaca
    26761 agtagcggcg ctggtattct cgattgatgc cccgcttaaa gtgctattag gtgatgctga
    26821 cagcaaatat attccagcca gtttatgtcg taccaacgct tctggtacgc ccgttaatgg
    26881 ctattttctg accctggtac tggtggcgat tctgattatg ctgccgactc tcggcattgg
    26941 tgatatgaac aatctctata aatggctgtt gaaccttaat tcggtagtga tgccgctgcg
    27001 ttatttatgg gtatttgttg catttattgc agtcgttcgc ctggcgcaga aatataaacc
    27061 agaatatgtc tttattcgta ataagcctct ggcaatgacc gtcgggattt ggtgttttgc
    27121 ctttaccgcc tttgcctgtt tgacggggat cttcccgaaa atggaagcct tcactgcaga
    27181 gtggaccttc cagttggcgc tgaatgttgc aacgccgttt gtgctggtag gattaggact
    27241 gatattcccg ctgctggcgc gtaaagcgaa tagtaaataa ttattgtggt cggtgacgct
    27301 gactctggca gatctgaccc gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca
    27361 tatactgg
//
LOCUS       DAJTVE010000047        26875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000047 DAJTVE010000000
VERSION     DAJTVE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..527)
                     /gene="acpT"
                     /locus_tag="OO439_001888"
     CDS             complement(<1..527)
                     /gene="acpT"
                     /locus_tag="OO439_001888"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6316263.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAI"
     gene            complement(582..1631)
                     /locus_tag="OO439_001889"
     CDS             complement(582..1631)
                     /locus_tag="OO439_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6316264.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1763..2980
                     /locus_tag="OO439_001890"
     CDS             1763..2980
                     /locus_tag="OO439_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6316265.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2984..3541)
                     /gene="dcrB"
                     /locus_tag="OO439_001891"
     CDS             complement(2984..3541)
                     /gene="dcrB"
                     /locus_tag="OO439_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6316266.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3614..4279)
                     /locus_tag="OO439_001892"
     CDS             complement(3614..4279)
                     /locus_tag="OO439_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6316267.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            4500..4745
                     /gene="tusA"
                     /locus_tag="OO439_001893"
     CDS             4500..4745
                     /gene="tusA"
                     /locus_tag="OO439_001893"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6316268.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4847..7045)
                     /gene="zntA"
                     /locus_tag="OO439_001894"
     CDS             complement(4847..7045)
                     /gene="zntA"
                     /locus_tag="OO439_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6316269.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(7119..7745)
                     /locus_tag="OO439_001895"
     CDS             complement(7119..7745)
                     /locus_tag="OO439_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6316270.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            7886..8245
                     /locus_tag="OO439_001896"
     CDS             7886..8245
                     /locus_tag="OO439_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6316271.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(8248..8517)
                     /locus_tag="OO439_001897"
     CDS             complement(8248..8517)
                     /locus_tag="OO439_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6316272.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8507..9103)
                     /gene="rsmD"
                     /locus_tag="OO439_001898"
     CDS             complement(8507..9103)
                     /gene="rsmD"
                     /locus_tag="OO439_001898"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6316273.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            9253..10749
                     /gene="ftsY"
                     /locus_tag="OO439_001899"
     CDS             9253..10749
                     /gene="ftsY"
                     /locus_tag="OO439_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6316274.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10752..11420
                     /gene="ftsE"
                     /locus_tag="OO439_001900"
     CDS             10752..11420
                     /gene="ftsE"
                     /locus_tag="OO439_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6316275.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            11413..12471
                     /gene="ftsX"
                     /locus_tag="OO439_001901"
     CDS             11413..12471
                     /gene="ftsX"
                     /locus_tag="OO439_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6316276.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12716..13570
                     /gene="rpoH"
                     /locus_tag="OO439_001902"
     CDS             12716..13570
                     /gene="rpoH"
                     /locus_tag="OO439_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6316277.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13841..14944
                     /gene="livJ"
                     /locus_tag="OO439_001903"
     CDS             13841..14944
                     /gene="livJ"
                     /locus_tag="OO439_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6316278.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(15143..15526)
                     /gene="panM"
                     /locus_tag="OO439_001904"
     CDS             complement(15143..15526)
                     /gene="panM"
                     /locus_tag="OO439_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6316279.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            15950..17059
                     /gene="livK"
                     /locus_tag="OO439_001905"
     CDS             15950..17059
                     /gene="livK"
                     /locus_tag="OO439_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6316280.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            17107..18033
                     /gene="livH"
                     /locus_tag="OO439_001906"
     CDS             17107..18033
                     /gene="livH"
                     /locus_tag="OO439_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6316281.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            18030..19307
                     /gene="livM"
                     /locus_tag="OO439_001907"
     CDS             18030..19307
                     /gene="livM"
                     /locus_tag="OO439_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6316282.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            19304..20071
                     /gene="livG"
                     /locus_tag="OO439_001908"
     CDS             19304..20071
                     /gene="livG"
                     /locus_tag="OO439_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6316283.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            20073..20786
                     /gene="livF"
                     /locus_tag="OO439_001909"
     CDS             20073..20786
                     /gene="livF"
                     /locus_tag="OO439_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6316284.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            21070..22134
                     /gene="ugpB"
                     /locus_tag="OO439_001910"
                     /pseudo
     CDS             21070..22134
                     /gene="ugpB"
                     /locus_tag="OO439_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            22232..23119
                     /gene="ugpA"
                     /locus_tag="OO439_001911"
     CDS             22232..23119
                     /gene="ugpA"
                     /locus_tag="OO439_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6316285.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            23116..23961
                     /gene="ugpE"
                     /locus_tag="OO439_001912"
     CDS             23116..23961
                     /gene="ugpE"
                     /locus_tag="OO439_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6316286.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            23963..25033
                     /gene="ugpC"
                     /locus_tag="OO439_001913"
     CDS             23963..25033
                     /gene="ugpC"
                     /locus_tag="OO439_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6316287.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            25030..25773
                     /gene="ugpQ"
                     /locus_tag="OO439_001914"
     CDS             25030..25773
                     /gene="ugpQ"
                     /locus_tag="OO439_001914"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6316288.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(25760..26200)
                     /locus_tag="OO439_001915"
     CDS             complement(25760..26200)
                     /locus_tag="OO439_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6316289.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            26320..>26875
                     /locus_tag="OO439_001916"
     CDS             26320..>26875
                     /locus_tag="OO439_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6316290.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVND"
BASE COUNT         6185 a         6951 c         7492 g         6247 t
ORIGIN      
        1 cagatcgcaa ggcttaaatt ttctagctga caatggctga ctgacagcga ggagtgatag
       61 gtgctatcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac gatggcttct
      121 ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatt
      181 tcagcgtgtt ccccgaggct gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg
      241 cgaatcactt cgatatcgca gccgacttcg ccttcatcgc tcaacagcag agcgatatcg
      301 tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct
      361 tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc
      421 gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt
      481 ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg
      541 tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt
      601 ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt ggtttccatc
      661 cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg gccgagcagc
      721 catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag gcgatggccc
      781 atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa caatgcgccg
      841 accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
      901 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
      961 agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc tgtccataaa
     1021 ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg gtgtaatcct
     1081 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
     1141 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
     1201 agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
     1261 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
     1321 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
     1381 gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
     1441 gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
     1501 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
     1561 aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt atcgggttga
     1621 gggatttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg agtcacgcca
     1681 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
     1741 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
     1801 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
     1861 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
     1921 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
     1981 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
     2041 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
     2101 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
     2161 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
     2221 gtggaacggc attgtcaatt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
     2281 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
     2341 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cactgccgtt
     2401 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
     2461 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt gggacggtgc
     2521 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
     2581 cggcattaac cgtatcggcg gcttaaacgt ggcgatgatt tgctttagcg ttgagataat
     2641 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaagtcg gcgtcttact
     2701 ggcgggggca gggttttcgc tggtgttccc ggcattgggc gtagtggcgg taaaagcggt
     2761 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
     2821 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
     2881 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
     2941 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
     3001 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct ggcagagtaa
     3061 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
     3121 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
     3181 ctttgttggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
     3241 tcgccaacac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
     3301 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
     3361 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
     3421 tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
     3481 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
     3541 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
     3601 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
     3661 tcaacagaat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
     3721 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
     3781 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
     3841 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
     3901 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
     3961 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
     4021 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
     4081 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
     4141 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
     4201 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
     4261 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
     4321 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
     4381 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
     4441 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
     4501 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
     4561 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcctggcgaa acgttgctga
     4621 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
     4681 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
     4741 gttgatgggg aaagattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
     4801 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
     4861 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
     4921 gccacaggcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc agccccagcg
     4981 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
     5041 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
     5101 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
     5161 catcaccgac catcgccagg ggcgcatgtt gattcagctt ggtcaccgct ttgactttat
     5221 cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt gccgccgctg
     5281 cacgtggatt atcgccggtg aggatcactc ctttgacccc cagcgcgttc agttcactga
     5341 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
     5401 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
     5461 caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct
     5521 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga atggcgagtt
     5581 cagcaacctg tgcttcgcgt acgatggctt gcgccagtgg atgcgtcgcg ccttgctcga
     5641 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
     5701 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
     5761 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgccgccg
     5821 ccagcccgga ggtaatcgcc gcaggcgttg agataactaa cgcacacggg cagccaatca
     5881 gcagcagcgt cagcccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
     5941 gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata cggctgaaac
     6001 ggtcgataaa ccgctcaatg ggggcgcgac gctcttcggc ttcttcgatc agtttcagaa
     6061 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
     6121 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
     6181 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
     6241 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
     6301 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
     6361 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
     6421 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
     6481 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgac
     6541 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
     6601 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
     6661 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
     6721 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
     6781 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
     6841 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
     6901 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
     6961 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
     7021 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
     7081 aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag tgagcggatg
     7141 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
     7201 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
     7261 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
     7321 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
     7381 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
     7441 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
     7501 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
     7561 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
     7621 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
     7681 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
     7741 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
     7801 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt atgcttattt
     7861 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
     7921 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
     7981 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
     8041 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
     8101 cgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
     8161 gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
     8221 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
     8281 cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg tcatctgcgg
     8341 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
     8401 aaaaatcagc gccacgttaa cgaaaatatt tagcgggcgc gggaagggat gcaccagatt
     8461 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
     8521 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
     8581 gatgtaacga ccagtttgcc ggaacggtgg gcagaccgtt ttcgacttcg ctttcgacat
     8641 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
     8701 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg cctttttgcg
     8761 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
     8821 gattcttaat taactgctga gaaactgcac gatccatctc aattaacgtg gcccccgcag
     8881 cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa tccagacatt
     8941 gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg
     9001 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
     9061 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
     9121 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
     9181 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
     9241 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
     9301 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
     9361 gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga agagcagccg
     9421 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
     9481 caggttgttg aaagtgaaaa agcgcagcct gaagcggaag ttgttgcaca gccggaaccg
     9541 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
     9601 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
     9661 attgtcaaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
     9721 gcacaggcgc tggcagccga agcagcagaa gaggcggtga tggtggttcc tccggcagaa
     9781 gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccaaccaa agaaggtttc
     9841 ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc cggatttatc
     9901 agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga agagcagctt
     9961 ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct gacggaaggc
    10021 gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa agaagagatg
    10081 ggtgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg
    10141 atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa gctggcacgt
    10201 cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt ccgtgcagca
    10261 gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat tgcccagcat
    10321 accggggcgg attccgcctc tgttatcttc gacgccattc aggcagctaa agcgcgtaat
    10381 atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca cctgatggaa
    10441 gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc gcatgaagtt
    10501 atgctgacta ttgatgccag caccgggcag aacgcggtaa gccaggccaa actgttccac
    10561 gaagctgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc gaaaggcggg
    10621 gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg tgtcggcgaa
    10681 cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact ttttgcccga
    10741 gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt gggagacagg
    10801 cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg accggtcatt
    10861 ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg
    10921 ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc
    10981 tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac cgtactgtct
    11041 acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat attcgtcgcc
    11101 gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac ttccctattc
    11161 agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg aataagcccg
    11221 cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg gaagggattc
    11281 tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca acgcacgaca
    11341 tcaacctgat ttcacggcgt tcccatcgca tgctcaccct gagcgatggt cacttgcatg
    11401 gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt tggtgggcgt
    11461 ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa cgcaccgaaa
    11521 cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa cgaacaggtg
    11581 cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc cacgttttta
    11641 acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat ggtgtacaaa
    11701 aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt ttacttgcaa
    11761 aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc
    11821 gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg taactggtct
    11881 ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt ggcggtggtg
    11941 atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga tcgtatcacg
    12001 cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg tctggcggcg
    12061 ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt ggcggcagtg
    12121 ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga ctccatcaac
    12181 gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct gtatggtggc
    12241 gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat tctggtgctg
    12301 cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt tgatatcaat
    12361 gatttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat tggctgggtg
    12421 gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata ataaaagcgt
    12481 gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt tgtctcttcc
    12541 cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt gcattgaact
    12601 tgtggataaa atcactgtct gataaaacag tggatgataa cctcgttgct cttaagctct
    12661 ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga tttgaatgac
    12721 tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct acatccgggc
    12781 agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg aaaagctgca
    12841 ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc ggtttgttgt
    12901 tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga ttcaggaagg
    12961 taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg tgcgcctggt
    13021 ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc gtaactggcg
    13081 tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc tgcgtaaaac
    13141 caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc gtgaactggg
    13201 cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg acatgacctt
    13261 tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc tctatctgca
    13321 ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag agcaggcggc
    13381 aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca tcatccgcgc
    13441 gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc gttacggtgt
    13501 ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc gcgctgctat
    13561 agaagcgtaa tttccgctat taagcagaga accctggatg cgagtccggg gtttttgttt
    13621 tttgggcctc tacaataatc aatttcccct ccggcaaaac gccaatcccc acgcagattg
    13681 ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag
    13741 tatgctgcta aagcacgggt agccatgcat aaaacgaaat aaagtgctga acaacaacat
    13801 cacaacacac gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc
    13861 gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa
    13921 agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc aagagtttac
    13981 cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag gcaacaaact
    14041 gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa
    14101 agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat caacgcagcc
    14161 tgcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcgcc
    14221 ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca
    14281 ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt
    14341 tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg gcctgaagaa
    14401 aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctctac
    14461 gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttaccaccc
    14521 ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg
    14581 gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct
    14641 gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca ttgttgacgc
    14701 gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct acgccgcgct
    14761 gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct
    14821 gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga aaggcgatct
    14881 gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga cagatgcgaa
    14941 gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt
    15001 catgccggat gcggcgtaaa cgccttatcc ggcctataag atccaaagaa atcagtaaat
    15061 tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc gttggtcatc
    15121 acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc gccgtaaatc
    15181 ccagtgcctg cataaacgct gtcatcacac cgcgatcttc cacgcctgca tccgccatcc
    15241 accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat tgccccacac
    15301 cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg gtgccgctta
    15361 aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag atacggtggt
    15421 tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc aggtcaatcc
    15481 ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt aacccgtgta
    15541 aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc tcttttttat
    15601 ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa acattagaga
    15661 atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc tttgtgccgt
    15721 tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc agaatattat
    15781 ctgttcttaa tcgactgaaa aatagggatt ttaatcactg ttatcacaaa atactgcgct
    15841 aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt
    15901 aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta tgaaacggaa
    15961 tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg ctatggctga
    16021 cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt ggggcgatat
    16081 ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg gaattaaggg
    16141 cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt
    16201 cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt gttcttcttc
    16261 tacccaacct gcatcagata tctacgaaga cgaaggtatt ctgatgatct cgccgggagc
    16321 gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg ccgggctgga
    16381 ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc cccagcgcat
    16441 cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg tgcaggacgg
    16501 gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg gggagaaaga
    16561 tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt actacggcgg
    16621 ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc tgaaaactca
    16681 gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgctg gcgatgctgc
    16741 cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa accagggcat
    16801 cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct ggatcaccta
    16861 cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg agccgctggc
    16921 gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc tgaactggga
    16981 tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg ccgatggttc
    17041 atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt ttagaaaggt
    17101 taccttatgt ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg cgtcacgctg
    17161 ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat tatcggcatg
    17221 atcaacttcg cccacggcga ggtttacatg attggtagct atgtctcctt tatgatcatc
    17281 gccgcactga tgatgatggg cattgatacc ggctggctgc tggtggcggc gggattcgtc
    17341 ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc ctatcgcccg
    17401 gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc catcttcctg
    17461 caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag tctgtttaac
    17521 ggtcagtggg tggtggggca tagcgaaaac ttctctgcct ctattaccac catgcaggca
    17581 gtgatctgga ttgttacttt cctcgccatg ctggcgctga cgattttcat tcgttattcc
    17641 cgtatggggc gcgcctgccg tgcctgcgcg gaagatctga aaatggcgag cctgcttggc
    17701 atcaacaccg accgggtgat tgctttgacc tttgttattg gcgcggcgat ggcggcagtg
    17761 gctggcgtac tgctcggtca gttctacggc gtcattaacc cctatatcgg ctttatggcc
    17821 gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc aggggcgatg
    17881 attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct gagtacggaa
    17941 tataaagatg tggtctcatt cgccctgctg attctggtgc tgctggtgat gccgaccggt
    18001 attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca atggcactgc
    18061 tctctgccgc gatgttcttt gtcctcgctg gcgtctttat gggcgtgcaa ctggagctgg
    18121 atggcaccaa actggtggtc gacagcgctt cggatgtccg ttggcagtgg gtgtttatcg
    18181 gcacggcggt ggtctttttc ttccagcttt tgcgacctgc tttccagaaa gggctaaaaa
    18241 gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg aagcagaaac
    18301 tgttcctcgt ggcgctgctg gtgcttgcgg tgtcgtggcc gtttatggtg tcacgtggga
    18361 cggtggatat cgccactctg accatgatct acattatcct cggtctcggg ctgaatgtgg
    18421 ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg tttttacgcc atcggcgctt
    18481 acacttttgc gctgctcaat cactattacg gtctggggtt ctggacctgt ttgccgattg
    18541 ccggactgat ggcagcggcg gcgggcttcc tgcttggttt tccggtgttg cgtctgcgcg
    18601 gtgattatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata ttgctgctca
    18661 ataacaccga aattaccggc ggcccgaacg gtatcagtca gatcccgaaa ccgactttct
    18721 tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc agtaatttct
    18781 ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg gcgttgctgc
    18841 tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt
    18901 gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc ccgcgtcgta
    18961 tcaagctgac tgcctttacc atcagtgccg cgtttgccgg ttttgccgga acgctgtttg
    19021 ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg gcgtttgtac
    19081 tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg gcggcaattt
    19141 tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg ttaatgctcg
    19201 gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc atgacgcgcc
    19261 cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc agccattatt
    19321 atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca acgtcaatct
    19381 tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac
    19441 ggtttttaac tgtctgaccg gattctacaa acccaccggc ggcaccattt tactgcgcaa
    19501 tcagcacctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg tgcgcacctt
    19561 ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg tggcgcagca
    19621 tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc
    19681 ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt tgctggaaca
    19741 cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg agattgcccg
    19801 ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag gtcttaaccc
    19861 aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc acaacaccac
    19921 tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc gaatttatgt
    19981 ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta ataacccgga
    20041 cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg tcctttgaca
    20101 aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg catatcaatc
    20161 agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc ttgctcggca
    20221 cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat aaagacatta
    20281 ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc
    20341 gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt tttgccgaac
    20401 gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gttcccacgt ctgcatgagc
    20461 gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg gcgattggtc
    20521 gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctt ggtcttgcgc
    20581 cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag gggatgacta
    20641 tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc
    20701 tggaaaacgg ccatgtggtg ctctccgata ctggtgatgc actactggcg aatgaagcgg
    20761 tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc aaaagactcc
    20821 tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag gcctactggg
    20881 tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt ttacgccgca
    20941 tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat ttttccgctt
    21001 atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt catctttctg
    21061 acaccttact tgcaggttta tgaagttggc accgccacca tgatggcatc gaaagccatt
    21121 aaaccggtat atgacgtgtt taaagaggca gggattcaat tcgatgagtc gcagtttgtg
    21181 ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc ccagccattc
    21241 aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc ggggttagac
    21301 ccggaacagc cgccgaaaac ctggcaggat ctggcggact cttccgcaaa actgaaagcc
    21361 tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact ggaaaacttt
    21421 agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg cacggacgcg
    21481 gtgctggagt tcaacaagcc ggagcaggtg aaacacattg ccatgctcga agagatgaac
    21541 aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa gttctataac
    21601 ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg ctaatattcg tgagtacgcc
    21661 aaatttaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga tgcaccgcaa
    21721 aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaaaataa agaaacgtat
    21781 accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc cgagtggcat
    21841 cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg tgagcagggc
    21901 ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa taagccgccg
    21961 ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt gattgtggat
    22021 gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact ggataccgcc
    22081 gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc ttaatcagtg
    22141 taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg ataagcgaag
    22201 cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg tgttccgctc
    22261 gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta tcttttttat
    22321 ctggcctgcg ggcgaagcgt tgtggtactc gcggcaaagc gtcgatccgt ttggtttctc
    22381 cagccagttt gtcggtctgg ataacttcgt cacgctgttc catgacagct actatctaga
    22441 ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt tgctggtatc
    22501 gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct atcaaacctt
    22561 gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga tcttcctgtt
    22621 taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg actggaacca
    22681 cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcggtgt ggaagcaaat
    22741 cagctacaac tttctgttct tctatgccgc gctgcactcc attccccgtt cgttgattga
    22801 agccgcagct attgacggcg cagggccgat tcgccgcttc tttaagattg ctctgccgct
    22861 tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct tcttcgacac
    22921 cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca ctacgctgat
    22981 ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg ccgcgcagtc
    23041 ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct atgttgaaag
    23101 caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag ccataccatg
    23161 ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt cgcggcgacg
    23221 ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac acatctgctg
    23281 gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc gttctggcgg
    23341 atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat taccgtctcg
    23401 atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct cttcttctgg
    23461 atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac ggtggaagtc
    23521 atcgccaacc tgaaaatgct cgacagctac gccggtttaa cgctgccgct gatggcctcg
    23581 gcgaccgcca cgtttctgtt ccgccagttc tttatgaccc taccggatga attggtggaa
    23641 gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt ttttccgttg
    23701 tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg gaatcagtat
    23761 ttgtggccgt tgttgattat taccgacgtt gatctcggca ccaccgtggc agggatcaaa
    23821 gggatgatcg ctacaggcga aggcaccacg gaatggaact cggtaatggc ggcgatgttg
    23881 ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt cgtgcgcggc
    23941 ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa ccaaaagctg
    24001 ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg gcgaatttat
    24061 cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg ttgccgggct
    24121 ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgaccg aaatggagcc
    24181 gaaagatcgc gggattgcga tggtgttcca gaattacgcg ctttatccgc atatgagcgt
    24241 agaggagaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc aaattgccga
    24301 gcgcgttaaa gaagcggcgc gcattctgga actggacggt ctgcttaagc gtcgtccgcg
    24361 tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg tgcgcgatcc
    24421 ggcggtgttc ttgtttgatg agccgctctc taacctcgat gccaagctgc gcgtgcagat
    24481 gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct acgttactca
    24541 cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg gtggcgttgc
    24601 cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt ttgtggcgag
    24661 ttttatcggc agcccggcga tgaacctgct gacaggccgc gtgaataatg aaggcacgca
    24721 tttcgaactg gacggcggta tcgtgctacc gctaaacggt ggctaccgtc agtatgccgg
    24781 gcgtaaaatg actctcggca ttcgcccgga acatatagcg ctaagctcgc aggcagaagg
    24841 tggtgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc tggcgcacgg
    24901 acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc cgacggcagg
    24961 cagcacgctg tggctgcatt tggcggaaaa tcagctgcat ctttttgatg gtgaaacagg
    25021 acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg cggcggtaag
    25081 ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg tcataagatg
    25141 atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca tgacgacaat
    25201 ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca ggatttactg
    25261 cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct gccgttgctt
    25321 tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc
    25381 accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg ccaactgtgg
    25441 gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga agctgcacaa
    25501 caggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga cgactggcgc
    25561 gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt actcgataaa
    25621 gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac cgtcaataaa
    25681 ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac cgatgcgatt
    25741 gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca ggctgcggcg
    25801 tgctgggctg attcagcatg tcgccgtttc tcctttccgg cagcatatgc tgctgattca
    25861 acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg ggcagcattt
    25921 gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga gaattattgt
    25981 ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt tgctgattca
    26041 gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc tgctggctgg
    26101 gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt gcaaagccga
    26161 caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct cctctgaaga
    26221 tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt tatataccag
    26281 gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc gacgttttta
    26341 cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc cgccgcgcct
    26401 cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt acgcgcgaaa
    26461 cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga tatcctcaag
    26521 gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc ggtaacgcat
    26581 ccgcaggcag ggaatctggg cggtggtggt tttatgttaa tccgctcgaa aaatggcaat
    26641 accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga tatgttcctc
    26701 gatgatcagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc ttccggcaca
    26761 ccgggtacgg tagcaggttt ctcgctggcg ctggataaat acggcaccat gccgctgaac
    26821 aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt ttatcgttaa cgacg
//
LOCUS       DAJTVE010000048        26827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000048 DAJTVE010000000
VERSION     DAJTVE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..924
                     /locus_tag="OO439_001917"
     CDS             <1..924
                     /locus_tag="OO439_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6316291.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO439_001918"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO439_001918"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6316292.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="OO439_001919"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="OO439_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6316293.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="OO439_001920"
     CDS             3885..4280
                     /locus_tag="OO439_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6316294.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="OO439_001921"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="OO439_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6316295.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="OO439_001922"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="OO439_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6316296.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="OO439_001923"
     CDS             complement(5589..6629)
                     /locus_tag="OO439_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6316297.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="OO439_001924"
     CDS             complement(6702..7337)
                     /locus_tag="OO439_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6316298.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="OO439_001925"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="OO439_001925"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6316299.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="OO439_001926"
     CDS             complement(7963..8406)
                     /locus_tag="OO439_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6316300.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO439_001927"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO439_001927"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6316301.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="OO439_001928"
     CDS             complement(8746..9249)
                     /locus_tag="OO439_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6316302.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="OO439_001929"
     CDS             complement(9243..9767)
                     /locus_tag="OO439_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6316303.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="OO439_001930"
     CDS             9976..10971
                     /locus_tag="OO439_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6316304.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="OO439_001931"
     CDS             10980..11858
                     /locus_tag="OO439_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6316305.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="OO439_001932"
     CDS             12064..13071
                     /locus_tag="OO439_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6316306.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO439_001933"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO439_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6316307.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO439_001934"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO439_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6316308.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO439_001935"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO439_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6316309.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO439_001936"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO439_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6316310.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO439_001937"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO439_001937"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6316311.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO439_001938"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO439_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6316312.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO439_001939"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO439_001939"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6316313.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO439_001940"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO439_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6316314.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO439_001941"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO439_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6316315.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO439_001942"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO439_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6316316.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO439_001943"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO439_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6316317.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6356 a         7449 c         6746 g         6276 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaa
//
LOCUS       DAJTVE010000049        26629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000049 DAJTVE010000000
VERSION     DAJTVE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..274
                     /locus_tag="OO439_001944"
     CDS             <1..274
                     /locus_tag="OO439_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6316318.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            367..450
                     /locus_tag="OO439_001945"
     CDS             367..450
                     /locus_tag="OO439_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316319.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            550..1371
                     /locus_tag="OO439_001946"
     CDS             550..1371
                     /locus_tag="OO439_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6316320.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1410..1739)
                     /gene="mdtI"
                     /locus_tag="OO439_001947"
     CDS             complement(1410..1739)
                     /gene="mdtI"
                     /locus_tag="OO439_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6316321.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1726..2091)
                     /gene="mdtJ"
                     /locus_tag="OO439_001948"
     CDS             complement(1726..2091)
                     /gene="mdtJ"
                     /locus_tag="OO439_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6316322.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2503..3537
                     /gene="tqsA"
                     /locus_tag="OO439_001949"
     CDS             2503..3537
                     /gene="tqsA"
                     /locus_tag="OO439_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6316323.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3562..4950)
                     /gene="pntB"
                     /locus_tag="OO439_001950"
     CDS             complement(3562..4950)
                     /gene="pntB"
                     /locus_tag="OO439_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6316324.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4961..6493)
                     /gene="pntA"
                     /locus_tag="OO439_001951"
     CDS             complement(4961..6493)
                     /gene="pntA"
                     /locus_tag="OO439_001951"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6316325.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7017..7961
                     /locus_tag="OO439_001952"
     CDS             7017..7961
                     /locus_tag="OO439_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6316326.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKTDAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8147..9529
                     /locus_tag="OO439_001953"
     CDS             8147..9529
                     /locus_tag="OO439_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6316327.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9566..10288
                     /gene="folM"
                     /locus_tag="OO439_001954"
     CDS             9566..10288
                     /gene="folM"
                     /locus_tag="OO439_001954"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6316328.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10285..10620)
                     /locus_tag="OO439_001955"
     CDS             complement(10285..10620)
                     /locus_tag="OO439_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6316329.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10749..11468
                     /gene="rstA"
                     /locus_tag="OO439_001956"
     CDS             10749..11468
                     /gene="rstA"
                     /locus_tag="OO439_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6316330.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11472..12773
                     /gene="rstB"
                     /locus_tag="OO439_001957"
     CDS             11472..12773
                     /gene="rstB"
                     /locus_tag="OO439_001957"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6316331.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12849..13778
                     /gene="tus"
                     /locus_tag="OO439_001958"
     CDS             12849..13778
                     /gene="tus"
                     /locus_tag="OO439_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6316332.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13775..15178)
                     /gene="fumC"
                     /locus_tag="OO439_001959"
     CDS             complement(13775..15178)
                     /gene="fumC"
                     /locus_tag="OO439_001959"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6316333.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15321..16967)
                     /gene="fumB"
                     /locus_tag="OO439_001960"
     CDS             complement(15321..16967)
                     /gene="fumB"
                     /locus_tag="OO439_001960"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6316334.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17166..18341
                     /gene="manA"
                     /locus_tag="OO439_001961"
     CDS             17166..18341
                     /gene="manA"
                     /locus_tag="OO439_001961"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6316335.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18442..19950
                     /locus_tag="OO439_001962"
     CDS             18442..19950
                     /locus_tag="OO439_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6316336.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20131..21441)
                     /gene="uidC"
                     /locus_tag="OO439_001963"
     CDS             complement(20131..21441)
                     /gene="uidC"
                     /locus_tag="OO439_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6316337.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21480..22853)
                     /gene="uidB"
                     /locus_tag="OO439_001964"
     CDS             complement(21480..22853)
                     /gene="uidB"
                     /locus_tag="OO439_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6316338.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22850..24661)
                     /gene="uidA"
                     /locus_tag="OO439_001965"
     CDS             complement(22850..24661)
                     /gene="uidA"
                     /locus_tag="OO439_001965"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6316339.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25050..25640)
                     /gene="uidR"
                     /locus_tag="OO439_001966"
     CDS             complement(25050..25640)
                     /gene="uidR"
                     /locus_tag="OO439_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6316340.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25861..26607)
                     /gene="hdhA"
                     /locus_tag="OO439_001967"
     CDS             complement(25861..26607)
                     /gene="hdhA"
                     /locus_tag="OO439_001967"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6316341.1"
                     /translation="MRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHV
                     VDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMA
                     DFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAAS
                     HLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIA
                     NAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6680 a         6909 c         6391 g         6649 t
ORIGIN      
        1 tatgggtctg tcttctgccg cctttgctgc agagactgcg accacacctg ctccgactgc
       61 gacgaccacc aaagcagcgc cggcgaaaac gacacatcat aaaaaacagc ataaagcagc
      121 acctgcccag aaagcgcagg cggctaaaaa gcatcataaa aatacgaaag ctgaacagaa
      181 agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc gggaaacaca gccatcagca
      241 accggcaaaa cctgctgcac aacccgcagc gtaagttttc aacagtaatg ctggcgcgcc
      301 ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt tagcaccgcc tttagcggag
      361 ggtagtatgg tgggccgtta tcgctttgag ttcattctta tcatccttat tttatgcgca
      421 ctgattgccg cccgttttta tctttcctga ctgtagttat ctgattttac tcccactttc
      481 atcccgtccc gtctatagta tttatgaggg tttgctttta ataatcataa ttacccacca
      541 gagtgtgata tgcgtacaac cattgctgta gtgttgggtg caattagttt gacgtctgct
      601 tttgtgtttg cagataaacc agacgttgcc aaatcggcaa acgatgaggt cagcaccctg
      661 ttttttggtc atgatgatcg tgtgccagtg aatgacacga cccaatcacc gtgggatgcg
      721 gttgggcaac tggaaacggc cagcggcaat ttatgtacgg cgacgctgat tgcacccaat
      781 ctggcattaa cggcaggaca ctgtttattg acacctccaa agggtaaagc cgataaagca
      841 gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct atgagatcca cgacatagaa
      901 ggccgcgttg atccgacact gggaaagcgg ttaaaagcag atggggatgg ttggattgta
      961 cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc tacgtaatcc cccttctggc
     1021 attacaccgt tgccgttatt tgagggagat aaagccgcgc ttactgccgc attaaaatcg
     1081 gctggccgta aagtaactca ggcgggatac cctgaagatc atctcgatac gttgtacagt
     1141 catcaaaact gtgaagtgac tggctgggcg caaacgtcgg tgatgtcaca tcagtgcgat
     1201 accttgccgg gtgacagcgg ttcgcctctg atgttgcata ccaatgacgg ctggcaatta
     1261 attggggtgc aaagttcggc tcctgccgcg aaagatcgct ggcgcgccga taaccgggcc
     1321 atttctgtta ccggttttcg cgacaagctg gatcaactgt cgcaaaaata atgttcaaac
     1381 gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt tcaccatgat cattccagcc
     1441 aacagcaaga ccaggccaat ccagccttta cgatttaacc gttgaccaaa caagatccaa
     1501 cctgcggcta acgtggcggc aataccaaac ccgccccaca atgcataagc gacagacaag
     1561 tcgatccctt taacggcctg agaaagcgca ctaaaggcag ccagcaccgc cgccagggag
     1621 agcaagccaa atattttgcg acgaaagccg tcagaaaatt tcaaaaagac gttagcaacg
     1681 atttccagca cgattgccaa tgccagccag gcggcgtgaa cccattcaaa ctgcgccatg
     1741 gttcacctcc agttcaggtt tacgcgcttt acgggtacct gattttatca acacaatccc
     1801 ggcgaccagg gtggttaacc cggcaatttt catcagcgat aaactttcgt cgaataacaa
     1861 aacgctaaac aaggtaataa ataaaatacc gataccttcc cacagcgcat aagctacgcc
     1921 taaggcgatc tttttaacgg cgaaagagag aaatatatac gacagagaaa tcatcaccag
     1981 cattaaaata aagccgccat ttccctcact gacgctcgcc catttcattg acagcgtacc
     2041 ggtaatttct gtagcaatag ccaggcctaa taaaatccaa taaatataca ttgtccttct
     2101 cctgcaagag aattatttta attttcgctt aattcagcga aaccaaagta aagtggtcaa
     2161 gccccgcgca tagggcaagc ttaggcagaa gaaaggacta aagcgcgttg cgccagtgct
     2221 gctcacctac gagcaagata gtggatgagg tacgaagatg ggtaaatgtt gaaaataacg
     2281 tcctgaacaa attgtccata atattacaat tatccgcagt gttgcttctc gtcatcgcgg
     2341 atgataattg tcctcggtag ttgaacacgc ctgatttgta tcatagctta agaattaact
     2401 caaaatattt tcacttcttt acctgagcgg tttgattttc gttatgatga cggagcgaaa
     2461 aagacattat tattagcaaa ggaagaaaaa acggggacaa gcatggcaaa gccgatcatc
     2521 acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc tggtcattat tctctgcggt
     2581 atccgttttg ccgccgagat catcgtgccg tttattctcg cattatttat tgctgttatt
     2641 cttaacccgc tggtgcaaca catggtccgc tggcgagtgc cgcgtgtact ggcggtgtcg
     2701 attttgatga ccatcatcgt gatggcgatg gtgttgctat tagcttatct gggttccgcg
     2761 ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact ctattatgac gccgctgcaa
     2821 gctcttgagc cgttgttgca acgcgtaggg attgacgtct cagttgacca gctggcgcat
     2881 tacattgatc cgaacgcggc gatgacgttg ctcaccaact tattgacgca gttatctaat
     2941 gccatgtcat caatattttt attgctgctg acggtgctgt ttatgctgct cgaagtgcca
     3001 caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg ttgaagggat ggcggcgatt
     3061 caacgtgcaa ttgacagtgt ttctcattat ctggtgctga aaacagccat cagcatcatc
     3121 accggcctgg tcgcctgggc gatgctcgcc gcactcgatg ttcgcttcgc ttttgtctgg
     3181 ggattgctgg cctttgcgct taattacatc cctaatattg gttcagttct cgcggcaatc
     3241 ccccctatcg ctcaggtact ggtgtttaat ggcttctacg aagcgttgct ggtgctggcg
     3301 ggatatctgc tgattaatct ggtcttcggc aatattctgg agccgcgcat catgggacgt
     3361 gggctggggc tttccacatt ggtggtattt ttgtcgttga ttttttgggg atggttgtta
     3421 ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta ttgtcaaaat tgcgcttgaa
     3481 caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg atctcaataa agagtgacgg
     3541 cctccgcaga ggccgtcagg gttacagagc tttcaggatt gcatccacgc tagctttggc
     3601 gtcaccaaac agcatgtggg tgttttcctt gaagaacagc gggttttgca caccagcata
     3661 gccagtgttc atcgaacgtt taaagacaat cacgttctgc gctttccaca cttccagcac
     3721 aggcatacca gcaataggac tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt
     3781 agcaccaatc accagtacgg tatcggtatc agcaaagtca tcgttgatct cgtccatttc
     3841 cagcacgatg tcatacggta cttttgcttc agccagcaat acgttcatat gtccaggcaa
     3901 acgccccgca accgggtgga taccgaaacg cacgttgata ccacgagcgc gcagtttctc
     3961 ggtaatttca gcgacaggat attgcgcctg cgcgactgcc atgccgtacc ccggagtaat
     4021 gatcactgaa tgggagtttt tcagcagttc cgctgtctct tctgcggtga tttcgcggtg
     4081 ctcgcccact tcctgatcat cgccagtaga agagccgtcg gtgccgaaac cacccgcaat
     4141 aacgctgata aaggaacggt tcatcgcctt acacataatg taagaaagga tagcccccga
     4201 agaaccgacc agcgcaccgg tcacaatcag caggtcgttg ctgagcataa agcccgcagc
     4261 cgcagccgcc cagccggagt acgagttcag catcgacacc accactggca tatctgcacc
     4321 accgatggag gcgactaaat gccagccgaa taccagcgca atggcggtca ttattagcaa
     4381 tgccagcact tgcaggccga cgctgtccgt gcgaacaaat acaatcagca gcaggaagga
     4441 aacgaccaga gccgccaggt tcattttgtg acggtttggc agcatcaacg gtttagacga
     4501 aatcttgcca cacagtttgc cgaacgccac caccgaaccc gtgaacgtta ccgccccgat
     4561 gaagataccg aggaacactt ccgtcaggtg aatattgacc agaatcggtg ccattcccgc
     4621 gtcatgatgc agatagctgt taaagccaac cagcactgcc gccagaccca cgaagctgtg
     4681 caggatcgcc accagttctg gcatttcggt catttcaact ttcttcgcca gacggatacc
     4741 aattgcccca ccaatgacca tcgccagcaa gatccagcca acattacccg tatccggtcc
     4801 aaaaatggtt gcgattaacg caatcgccat cccggcgata ccgaagttgt taccctggcg
     4861 agacgtttca tgtttcgaaa gaccggccag actgaagata aacaggatcg cggcaacaat
     4921 gtatgcagct gtaactaatc ctccagacat atgttacccc ttaatttttg cggaacattt
     4981 tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt aatgctggct ataagcaccg
     5041 cgataaaact aaggaagcta acccagccgc cctggccaat ctgcaacagt gctccgacaa
     5101 caataatccc tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc gcgtgcgata
     5161 cattccacac cacgtaataa ccgacaacgc aggccagcgc gaaaacggtg aagtgcccaa
     5221 gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag gatgattgcc agcgccatca
     5281 acgcgtattt acgccacggt gagcaggcac atttttcctc agttttcact tccggtgccg
     5341 ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg cggtgccggc caggtaattt
     5401 cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc atcaaaatca acagtgatat
     5461 tgccgtcttt ctctttgcac aacagtttca gcagattaac gaggttagta ccgtaaagct
     5521 gcgaggattg cgtcggcaga cggcccggaa gatcggtata accaatcact ttgacaccat
     5581 tttccgtagt gaagatttca cccggcacgg tgtattcaca gttgccaccg ttttgggctg
     5641 ccaggtcgac aatcacactg cccgccttca tggagtcaac catttcacgg gtaattagct
     5701 tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat gatatcgacc tcttttgcct
     5761 gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc cgacatcact ttggcatagc
     5821 catcgccact gcccgcttcc tctttaaaat ccagctcgag gaattccgcg cccatacttt
     5881 gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg cacaatcgcg ccgagactgt
     5941 ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc aatcaccatc acttttgccg
     6001 gtggcacttt cccggccgca gtaatttgcc cggtaaagaa gcgcccaaat tcatgtgccg
     6061 cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga gcttagtgcg tccagcgatt
     6121 gtgcgcgtga gatacgcggc acagaatcca tcgccatcac ggtcacgtta cgttccgcaa
     6181 gtttttgcat taattccgga ttctgcgcag gccagataaa actcaccagc gttgtcccag
     6241 gattcagtaa cgcaatttca tcatctaacg gcgcattgac cttcagaatg atctctgact
     6301 gccagacgct attcccttct acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa
     6361 aacttgccag ttgacccgcg ccgctctcta ccgcgacggt aaaacccagt ttcagcagct
     6421 gttccactgt ttttggcgtt gctgcaacac gggtttcatt ggttaaccgt tctcttggta
     6481 tgccaattcg catgatattc ccttccatcg gttttattga tgatggtttg cctgtgtcag
     6541 gagccacaca agccgctcat gtacgagcta aatgttactc cgttaaaata aattagtaac
     6601 aaacgcccta taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc
     6661 ggcgtgaact cgcaaaatat taacgattca gccgtgatag tgggataaac accttagaac
     6721 gccggataaa gactgataat tgtcttcgac ggccgggtaa aacgagacat cgccccggca
     6781 cgattcacta cttaacatta aattaactta tacaattcag ttgcttcagt agtaatgatg
     6841 ctgatacggc tgttttttaa gcatagacgg tcatttgagc aggattaaaa ttggcttaag
     6901 gaatgtgata tgaaaaatga cgcagacagt tacaccgttt aaatgcaata atcagccacg
     6961 tttctcgtta ataacaatac cagtacctgg tttgcgcaag gcgaaggatt atttttatga
     7021 agcttaagaa caccctcctg gcgtcggcac tgctttctgc tacggcattc tccgttaacg
     7081 cagcaacaga actgacaccg gagcaagcgg cagcggttaa accttttgac cgtgtagtgg
     7141 ttaccggtcg ttttaatgct attggcgaag cggtgaaagc cgtttctcgt cgcgcagata
     7201 aagaaggtgc cgcctctttt tatgttgtcg acacttctga ttttggtaac agcggtaact
     7261 ggcgtgtggt cgctgacctc tataaaaccg atgctgaaaa agcagaagaa acaagtaatc
     7321 gcgtaattaa cggtgttgtc gaactgccga aagatcaggc tgttctgatt gaaccgtttg
     7381 acacggtcac cgtccagggc ttctatcgta gccagccaga agtcaatgat gccatcacca
     7441 aagcggcaaa agcgaaaggt gcctactctt tctacatcgt tcgtcaaatc gatgccaacc
     7501 aaggcggcaa ccagcgtatt actgcattca tctataaaaa agatgctaag aaacgtatcg
     7561 tccagagccc ggatgtgatc ccggcagatt ccgaagcagg acgtgcagct ctggctgccg
     7621 gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc gactaccgca tcaccaagtt
     7681 ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg cgggcgttac accgtcatgc
     7741 tcccggatgg cactaaagtc gaagaactga acaaagcgac cgcagcgatg atggtcccgt
     7801 tcgacagcat caaattctct ggcaactacg gcaacatgac cgaagtctcc tatcaggttg
     7861 cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat cacccgccag tggcaggaac
     7921 gtggtaataa cctgaccgtc agcgcagatc tgtataaata actattcagt tactcctata
     7981 aggcggcttg atgccgcctt tcgcattttt agtcacaaat ttccccgcga ccattgcatg
     8041 cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa actccatttt tatgcgtttt
     8101 gccctaatat ttattcttct tatcacgttt taatcactgg atatcgatga aaaagaaact
     8161 gggactgagc gcactcaccg cgctggtatt aagctcaatg ctgggcgcgg gtgttttcag
     8221 tctgccgcaa aatatggcgg cagttgccag cccggcagca ctgctcatcg gctggggtat
     8281 tactggcgct ggcattttat tgctggcctt tgccatgctg atcctcacgc gcattcgtcc
     8341 cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg tttggcgagc taatcggctt
     8401 ttgttccgca tggggatact ggctgtgcgc agtcatcgcc aacgtctcct atctggtgat
     8461 cgttttttcc gcgttaagct tttttacgga cacgccggaa ttgcgcctgt tcggcgatgg
     8521 taatacctgg caatcgatcg tcggtgcatc ggctttattg tggattgttc actttttgat
     8581 cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg gcgacactgg caaaattgtt
     8641 gccgctgggt ctgtttgttg tgctggcgat gatgatgttc aaactggata ccttcaagct
     8701 cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa caggtgaaaa acaccatgct
     8761 gatcaccctg tgggttttca ttggtgtgga aggtgccgtg gttgtttctg cgcgtgcgcg
     8821 taataaacgt gatgttggca aagcgacact gctggcggtt ctctccgctc tgggcgttta
     8881 cttgctggta acgctgcttt cgctgggtgt ggtggcacgt cctgaactgg ctgaaattcg
     8941 taacccgtca atggccggtc tgatggtgga aatgatggga ccatggggcg aaatcatcat
     9001 cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta agctggacca tcatggcggc
     9061 agaagttccg ttcctggctg ccactcataa agcattccca cgcatttttg cgcgccagaa
     9121 cgcacaagct gcgccatccg cctcactgtg gttgaccaac atctgcgtgc aaatttgtct
     9181 ggtactcatc tggctgaccg gttccgatta caacacgttg ctgaccatcg cctcagaaat
     9241 gattctggtg ccctatttcc tggtcggcgc gttcttgctg aaaatcgcaa cccgtccact
     9301 gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta tggttattgt acgcctctgg
     9361 tccaatgcac ttgctgttat ccgttgttct gtatgcgccg gggctgctgg ttttcctgta
     9421 tgcgcgcaaa acgcatacac atgataatgt actgaaccgt caggaaatgg tgctgattgg
     9481 catgctgtta attgcctcag ttccggcgac ctggatgctg gtgggataac gttcatccca
     9541 tcgttggata gcaaaggaga taacgatggg taaagcccag cccttgccaa tattaattac
     9601 tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat ttcattaatc aaaagcaacc
     9661 ggtgattgtc agctatcgga cgcactatcc agccattgat ggactgatta aggcaggtgc
     9721 gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg atggcgtttg ccgatgaagt
     9781 actaaaaagc ccccatggtc tgcgtgctat tttgcataac gccagtgcgt ggatggcgga
     9841 aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg atgcagatcc acgttaatac
     9901 cccatacctg ctcaaccatg cgctggaaag attactgcgt gggcacggac acgccgccag
     9961 cgatatcatt cactttaccg attatgtggt ggagcgcggt agcgacaagc atattgcgta
    10021 tgctgcaagc aaagcggcac tggataatat gacccgctcg tttgcccgca agctggcacc
    10081 ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg tttaatgaac atgatgatgc
    10141 cgaatatcga caacaggcgc tgaataaatc actgatgaaa accgcgcctg gcgagaaaga
    10201 agtgatcgac ctggtcgatt acttacttac cagttgcttt gtcaccggac gcagtttccc
    10261 acttgatggc ggtcgtcatc tgcgttaatg cagctttatc cagcatataa tcaacaccca
    10321 tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg gcgggcaagc gcatcattcc
    10381 ggtgaaatac cacagcgaca ccaggtaaac aaaataggga ataatcgacc acatactaaa
    10441 aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg gcaacaatat aatgcgcgat
    10501 aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata taataatttt tcgtttttgc
    10561 taaaacacca atcaacagca ctaccagcgc accgagcgcg gctttgatta ccagccccat
    10621 ttttttacct taacacttcc ataacaagtc atcagtagaa tacctgatga aaacttgttt
    10681 agaaacgatt gatagtaagt aaaaacagcg cggtgtattg tgacgttttt atatctaccg
    10741 tgaatgttat gaacactatc gtatttgtgg aagatgatgc ggaagtcggt tcactgattg
    10801 ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga gccgcgcggc gaccaggccg
    10861 aagaaaccat tttgcgagaa aatccggatt tggtgttact cgacatcatg ctaccaggca
    10921 aggacggcat gaccatttgt cgtgatttac gcgcaaagtg gtctggaccg attgttcttc
    10981 taacctctct cgatagcgat atgaaccaca tcctggcact ggaaatgggt gcctgcgact
    11041 atattctcaa aacgacgccc cctgctgttt tgctagcgcg tttacgtttg catttgcgtc
    11101 agaatgagca agccacactg accaaaggtc ttcaggaaac gtctctgact ccctacaaag
    11161 ccctgcattt cggcacgttg accatcgatc ccatcaaccg cgtagtcacc ctggctaaca
    11221 ctgaaatctc gctctcgaca gctgatttcg aattattgtg ggaattagct acccatgccg
    11281 ggcaaatcat ggaccgcgat gcattgctga aaaatttacg cggcgtcagt tatgacggac
    11341 tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa aaaactgctc gataacgccg
    11401 cagaacctta tcgcattaaa actgtgcgta acaaaggcta tctttttgcg cctcatgcat
    11461 gggaataagc gatgaaaaaa ctgtttatcc agttttacct gttattgttt gtctgcttcc
    11521 ttgtgatgtc tctgctggtt gggctggtgt acaaatttac cgccgaacgc gcgggcaaac
    11581 agtcgctgga tgatttgatg aacagttcac tgtatctgat gcgcagcgaa ttgcgtgaga
    11641 tccccccaca cgactggggt aagacgctga aagagatgga tttaaatctc tctttcgatc
    11701 tgcgtgtcga gccactgagt aaataccatc ttgatgatat ttccatgcac cgactgcgtg
    11761 gcggcgaaat tgtcgccctg gacgatcagt acacgttttt gcagcgtatc ccgcgcagcc
    11821 actacgtgct ggcagttggt cctgttcctt atctttatta tctccatcag atgcgattgc
    11881 tggatatcgc cctgatcact tttattgcta tttccctcgc ctttccggtg tttatctgga
    11941 tgcgtccgca ctggcaggat atgttaaaac tggaagcagc ggcgcaacga tttggcgatg
    12001 ggcatctcaa tgaacgtatc cactttgatg agggttcgag ctttgaacga cttggtatcg
    12061 catttaacca gatggcggac aatatcaacg ccttgattgc cagcaaaaaa cagcttattg
    12121 acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga ctggagatga
    12181 gcgataacct gagcgccgcc gaatcccagg cgttgaatcg tgatatcagt caacttgaag
    12241 ctttaattga agagctgctg acttatgccc gactcgatcg cccacaaaac gagcttcatc
    12301 ttagcgaacc agacctgccg ttgtggctgt caacgcatct ggcagacatt caggcagtaa
    12361 cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg ccattatgcg gcgttggata
    12421 tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa cgccctgcgg tactgccatt
    12481 caacggttga aaccagcctg ctactgtcgg ggaatagagc gacattaatt gttgaggatg
    12541 atggcccagg gattgcccca gaaaaccgcg aacatatctt tgaacctttt gttcgcctcg
    12601 atcccagccg ggatcgctca accggcggct gcgggctggg gctggcaatt gtccactcta
    12661 tagcactggc aatgggcggt acggttaatt gtgacaccag cgaactgggt ggtgctcgct
    12721 tctcgtttag ctggccgtta tggcataaca tcccgcaatt tacctctgcc tgacactacg
    12781 cgcacgatgg tcaagtcacc accactgtgc tataaaataa gtatgttgta actaaagtgg
    12841 ttaatattat ggcgcgttac gatctcgtag accgactcaa cactaccttt cgccagatgg
    12901 aacaagagct ggctgcattt gccgctcatc ttgagcaaca caagctattg gttgcccgcg
    12961 tgttctcttt gccggaggta aaaaaagagg atgagcataa tccgcttaat cgtattgagg
    13021 taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt gcgtcatttc cgccatttat
    13081 ttattcaaca acagtccgaa aatcgcagca gcaaggccgc tgtccgtctg cctggcgtgt
    13141 tgtgttacca ggtcgataac ctttcgcaag cagcgttggt cagtcatatt cagcacatca
    13201 ataaactcaa gaccacgttc gagcatatcg tcacggttga atcagaactc cccaccgcgg
    13261 cacgttttga atgggtgcat cgtcatttgc cggggctgat cacccttaat gcttaccgca
    13321 cgctcaccgt tctgcacgac cccgccactt tacgctttgg ttgggctaat aaacatatca
    13381 ttaagaattt gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg aaatcaccac
    13441 gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa actggagcga gagtatcagg
    13501 atatcgctgc cctgccacag aacgcgaagt taaaaatcaa acgtccggtg aaggtgcagc
    13561 cgattgcccg cgtctggtac aaaggagatc aaaaacaagt ccaacacgcc tgccctacac
    13621 cgctgattgc actgattaat cgggataatg gcgcgggcgt gccggacgtt ggtgagttgt
    13681 taaattacga tgccgacaat gtgcagcacc gttataaacc acaggcgcag ccgcttcgtt
    13741 tgatcattcc acggctgcac ctgtatgttg cggattaacg cccggctttc atactgccga
    13801 ccatctgttc tggccgtacc cagctgtcaa actcggcttc gctaagatac cccagcgcaa
    13861 gggccgcagc ttttaaggtc agcccttctt tatgcgcttt tttggcgatc tccgcggctt
    13921 tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat cagcgattca ttgagtaatt
    13981 gattgattcg ctcacgattc ggttcaatac ccactgcgca gtgtttgtta aaactttcca
    14041 tgccatctgc cagcaagcgc accgattgca ggaaattgtg gatcaccatt ggacggaaga
    14101 cgttcagttc aaagttaccg gaagcgccac ccatgttgat cgccacgtcg ttccccatta
    14161 cctgacagca gagcatggtt aatgcttcac actgggttgg gttcactttc cccggcatga
    14221 ttgagctgcc cggctcattt tccgggattg agatttcacc aattccgcag cgcgggccag
    14281 aggccagcca gcggacatca ttggcgattt tcatcagtga cgcagccaac cctttcaacg
    14341 cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc ttcaaatttg ttcggcgcgg
    14401 taacaaacgg tgcacaggta atgactgcca gttcatctgc tacgcgacgc gcatactccg
    14461 gatgggtatt tagtccagta cccaccgctg taccgccgag agccagttcc gctacgtgag
    14521 gcaggctgta ttcgatatgt ttgagattat gctcgagcat cgctacccag ccggaaatct
    14581 cctgccccag tgttagcggc gtggcgtcct gcaagtgggt tcgaccgatt ttgacgatat
    14641 cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag ggttttaacc tgcggaatga
    14701 gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc cgtcggaaag acatcgttgg
    14761 aactttggct tttgttcacg tcgtcgttag ggtgaacttt acgttccatc ccgcgcacgc
    14821 cgccgagtaa ttcactggcc cggttagcca gcacttcgtt catgttcatg ttactttgcg
    14881 tgccggagcc ggtctgccag atagccagcg ggaattcgtc gtcatgctgt cctgccagta
    14941 cttcatccgc cgcctgccga atggcgctcg ctttctcttc agacaacaag cctaaatctt
    15001 cattaacttt tgccgctgcg cgcttggtta gcgccagcgc atgaatcagt gaggtgggca
    15061 ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg ccccacagct
    15121 tatctgccgg gacatcaatc gcccccatcg aatctttttc gctgcgtact gtattcatgg
    15181 cctgctcctc acctgattaa ttttttcttt ctgttttgcc ttcgttaagc aacttttagc
    15241 tcacttatta tttactattt gataacaaat gtttggtctt tcgtgccatg taaaaaaacc
    15301 gccccgaagg gcggctctgt ttatttcaca cagcgagtgc actgtgtgag ttgtatctgc
    15361 tggaagaagt catttccttt atcatccaca aggataaacg ccgggaaatc ttccacttca
    15421 attttccaga tggcttccat tcccagttcc ggatattcaa cacattccag gctcttaata
    15481 cttccctgcg ccaatacagc ggccggacca ccgatactgc caaggtagaa gccgccgtgt
    15541 tttttacagg catccgtcac ctgctggctg cggttgcctt tcgccagcat gatcatactt
    15601 ccgccctgcg cttgcagttg atcgacataa gaatccatcc gtccggcggt cgttgggcca
    15661 agagaaccgg aggcataacc ttccggcgtt ttggccggac ccgcgtagta aatcggatga
    15721 tctttaatgt actgcggcag cccttcaccg ttatccatcc gctctttcag tttggcgtga
    15781 gcaatatcac gaccgacgat aatcgtgccg ttaagcgata agcgtgtaga aacgggatac
    15841 tgcgacaact gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg caccgcttcg
    15901 ccttctcccg ctttacgcag ctcttcaggg atatatttac ctggattatg ttccagtttt
    15961 tcgatccaga tcccctgacg gttgatcttc gctttgatat tacggtcagc agagcaggaa
    16021 acacccatac caaccggaca ggatgcgccg tgacgtggca ggcgaatcac gcgtatgtcg
    16081 tgagcgaagt atttaccacc aaactgcgca cccagaccga gattttgcgc ttcgatcagc
    16141 aattcttttt ccagttccac atcgcggaat gcctgaccgt gctcattccc ttccgttggc
    16201 agttcgtcat agtatttagc ggaagccagt ttcactgttt taaggttagt ttctgcagaa
    16261 gttccaccaa ttacgaacgc aatatgatac ggaggacagg ccgccgtacc cagcgtgcgc
    16321 atcttctcaa ccaggtaatt tttcagtttc cccggcgtta gtaacgcttt ggtttcctga
    16381 tagagatacg tcttgtttgc cgaaccacca cctttggcga tacagaggaa tttgtactcg
    16441 tcgccatcaa cggcataaag atcgatctgc gctggcagat tggtgccggt attcacctct
    16501 ttatacatat ccagcggcgc gttttgcgag tagcgcaaat tatcttcgat ataagtgtta
    16561 tagacaccgc gcgccagcgc cgcttcatca cagccaccgg tccatacacg ctgccctttt
    16621 ttaccaacaa taatcgcagt gccggtatcc tgacaggttg gcagaacacc tttcgccgcg
    16681 atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat cattttcgct ggcttccggg
    16741 tcacgcagaa tgtcggccac ctgttgttgg tgagccggac gcagcataaa tgacgcatca
    16801 tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg cgactttcaa aatctcctgc
    16861 ccttcaaatt cagatacgct aacgtgttcg ctggttagca ggtaatactc agtatcatct
    16921 tttttgagtg gaaaaggagc ctgataatga aagggtttgt ttgacattgt tctctcactt
    16981 actgcctggt ttggttatgc tctgggcggg tgttccgttg ccctgttaaa agcgagtaac
    17041 aatatcctac acactttttt aacaaaaact gagactagta cgactttttg cggctccagg
    17101 ttacttcccg taggattctt gctttaatag tgggattaat ttccacatta aaacagggat
    17161 tgatcatgca aaaactcatt aactcagtgc aaaactatgc ctggggcagc aaaacggcgt
    17221 tgactgaact ttatggtatg gaaaatccgt ccagccagcc gatggccgag ctgtggatgg
    17281 gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc cggagatatc gtttcactgc
    17341 gtgatgtgat tgagagtgat aaatcgactc tgctcggaga ggccgttgcc aaacgctttg
    17401 gcgaactgcc tttcctgttc aaagtattat gcgcagcaca gccgctctcc attcaggttc
    17461 atccaaacaa acgcaattct gaaatcggtt ttgccaaaga aaatgccgca ggtatcccga
    17521 tggatgccgc cgagcgtaac tataaagatc ctaaccacaa gccggagctg gtttttgcgc
    17581 tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc cgagattgtc tccctactcc
    17641 agccagtcgc aggtgcacat ccggcaattg ctcacttttt acaacagcct aatgccgaac
    17701 gtttaagcga actgttcgcc agcctgttga atatgcaggg tgaagaaaaa tcccgcgcgc
    17761 tggcgatttt aaaatcggcg ctcgacagtc agcagggtga accgtggcaa acgattcgtt
    17821 taatttctga attttacccg gaagacagtg gcctgttctc cccgctgtta ctgaatgtgg
    17881 tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac gcttacctgc
    17941 aaggcgtggc gctggaagtg atggcgaact ccgataacgt gctgcgtgcg ggtctgacgc
    18001 ctaagtacat tgatattccg gaactggttg ccaatgtgaa attcgaagcc aaaccggcta
    18061 accagttgtt gacccagccg gtgaaacaag gtgcagaact ggacttcccg attccagtgg
    18121 atgattttgc cttctcgctg catgacctta gtgataaaga aaccaccatt agccagcaga
    18181 gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt gtggaaaggt tctcagcagt
    18241 tacagcttaa accgggtgaa tcagcgttta ttgccgccaa cgaatcaccg gtgactgtca
    18301 aaggccacgg ccgtttagcg cgtgtttaca acaagctgta agagcttact gaaaaaatta
    18361 acatctcttg ctaagcttat taaaggctta taacaccttc aggcggccag tccgcctgat
    18421 ttcattttat ggataatcat tatgaataaa tcgctggtag cggtaggcgt cattgttgcg
    18481 ctaggcgtag tctggacagg cggcgcatgg tatacaggca agaagattga aacccatctc
    18541 gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga cagctcctga atccaacctg
    18601 gaagtgagtt atcaaaacta tcatcgcggc gtattcagca gccagttgca actgttggtg
    18661 aaacccattg ccgggaaaga aaatccgtgg attaaaagcg gtcagagcgt catcttcaac
    18721 gaatcggttg atcatggtcc cttcccgctt gcccagctta aaaaactgaa cctgatcccg
    18781 tcgatggcat caattcaaac cacgctggtt aataacgaag taagcaaacc actgtttgat
    18841 atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca ttggttacag cggtgattcc
    18901 agttccgata tttcgctcaa gccactgaat tacgagcaaa aggatgaaaa agtcgccttt
    18961 agcggcggcg agttccagtt aaatgctgac agagacggca aagccatctc cctttccggg
    19021 gaggcgcaaa gtggtcggat agacgcagtt aacgaataca accagaaagt gcagttgacc
    19081 tttaataatc tgaaaaccga cggttccagc acgctggcaa gttttggtga gcgcgtagga
    19141 aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg aaggcaaaga actggcactg
    19201 ctggaaggca tggagatcag cggtaaatcg gatctggtca atgacggtaa aacgatcaat
    19261 agccaactgg attactcgct aaacagcctg aaggtacaga atcaggatct gggcagcggc
    19321 aagctgactt taaaagtcgg ccagattgat ggtgaagcct ggcatcagtt tagccagcaa
    19381 tataacgcgc aaactcaggc gctgctggca cagccagaaa ttgccaacaa tcccgaactt
    19441 tatcaggaga aagtgacgga agccttcttt agcgccctgc cgctgatgtt gaaaggcgat
    19501 ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc agggtgaaag tgcgctgaat
    19561 ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag cgccgcaaac gctggcgcag
    19621 gaagtagatc gttcggttaa atctctggat gcgaaactga ccattccggt ggatatggca
    19681 actgagttta tgactcaggt agcgaagctg gaaggttatc aggaagatca agcgaaaaaa
    19741 ctggcgaaac agcaagttga aggtgcatca gcaatggggc agatgttccg tctgaccacc
    19801 ttgcaggaca ataccatcac caccagcctg caatatacta acggtcagat aacgttaaac
    19861 gggcagaaaa tgccactgga agatttcgtt ggtatgtttg cgatgccagc tcttaacgtt
    19921 ccggctgtac cggcaattcc gcagcagtaa ttcacggcca aaacgccgga gaattttcac
    19981 ttcggcgttt tcatctgaaa acaaaccaga gattgcctga tgcgctacgc ttatcaggcc
    20041 taccttttcc tgcaacactt tgaatttatg agtttttgta ggctggataa ggcgtttaca
    20101 ccgcatccgg cataaaaaac gcgcactttg tcaacaatct gaaacgccgg aggtttttct
    20161 ctccggcgtt atttctgact tcagcataaa gtcatactga atactcattt tcacatccca
    20221 gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc
    20281 aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa
    20341 gatacccgcg ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat
    20401 ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag ttgacgccca attgccagga
    20461 ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc
    20521 caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca
    20581 cggcgcattc caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt
    20641 acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt
    20701 cgggtcgagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg
    20761 taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga
    20821 acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat
    20881 accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat
    20941 atttggtgtt aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg
    21001 cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc
    21061 gaagcgacct gatttaagca ggctattttc actgggtttt atttttactg cccccagggc
    21121 aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc
    21181 taatttataa acataatatg ccccgccttc aacgccaata atattggaat aataaccact
    21241 attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact
    21301 gggcttatcc gccctgcgca attcgttttt cagtctgatg cgtagtcccg cctcatcgtc
    21361 agccagttgc gccgcataag cagagataag gccagaggca gccgtcaggc aaataacggc
    21421 catggccact atttttctca tagttgcacc tttgatgttc tgattatttt tattgattat
    21481 taattagtga tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc
    21541 acgatttctt tgaatttttt atccgtaagc ggataaaacc agataataac gaatgccagt
    21601 agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc
    21661 gtttgcacct gattggcaat atatccgctt aaccccaaaa taaaggcagg aattgaacct
    21721 ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt
    21781 cgcacgccgg tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcact
    21841 gtcatagtaa cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt
    21901 gaccagacgg aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc
    21961 aggaaggtat tctttttacc gatcctcgcg accatccccg gcacaagcgg tgccgatgcc
    22021 acagtaccaa ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac
    22081 acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa aggtcgaaat cagcacacac
    22141 agcgcaccga tgcacaacat aaacagcggg cgattccgtt tcagggtttg caaactgatc
    22201 ttcaatgacg gctgcgcaac gatacgtacc acattctcac gcgtcgattt gaagcagatg
    22261 aagtaaagca ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc
    22321 atctcttccg ggctggagtt cttaatgctc ggccctatca gaaatgccag gcagacaaag
    22381 gtcaatgaag cggcaatccc acgagccgcg cccagacggg cgcgggattg tggttgttgg
    22441 gtcatcgcgg tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg
    22501 cccatgtagg tcaaatatgc atacactact ttgcggctat ggctccagtc ggtcggtacc
    22561 cagaatacca gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg
    22621 aattttcccc agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag
    22681 gcatcgaata cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg
    22741 acgtcggtgt agtaactcaa caggaagagc acccccattg cgaaggcgaa gttattggcg
    22801 acgtcaccga ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc
    22861 ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag
    22921 ccgccgactt cggtttgcgg tcgcgggtga agatcccctt cttgttaccg ccaacgcgca
    22981 atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc
    23041 tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc
    23101 acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga
    23161 taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg
    23221 ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg cacagcacat
    23281 caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga
    23341 tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg
    23401 caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt
    23461 ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga gtttccccgt
    23521 tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta
    23581 aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg
    23641 cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa
    23701 tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca
    23761 agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga
    23821 actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact
    23881 ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga
    23941 ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca accacctgtt
    24001 gatccgcatc acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag
    24061 acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt
    24121 tcggcgtggt gtagagcatt acgctgcgat ggatcccggc atagttaaag aaatcatgga
    24181 agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct
    24241 gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat
    24301 acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat
    24361 tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc
    24421 ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc gcataattac
    24481 gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt
    24541 cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga tccagactga
    24601 atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca
    24661 taagggactc ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa
    24721 ttctgtgttg cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga
    24781 actgacaccg cgatcaaaaa accaaaaact gcaataccca cagcgtgata ttgatcgcat
    24841 taatctttaa aacggcgttg acagaagcca taaaataagc aaccgaacgt tcgttcgagc
    24901 atattgggaa cgataaactc tcatgacata tggctacggt gaatattttt gggggggaat
    24961 tctccgatcc cggctagatt gtattttgta cagggaaatt aaagtatgaa acgcgggcac
    25021 agttatcacc agcccgcgaa atgagaggat caggatgcgg ttaagatacc gccaatcatc
    25081 gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat cttccgcttc gatatcggcc
    25141 agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca ttgacgtgtt gatgtctggg
    25201 cttatctcgc ctttttgctg ggcatcacgc atccgctggg caacaaattc cgtgatcgtc
    25261 atatgcttat ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt
    25321 tcgaccacca gcgcccgttg cccaaaggct tcatgggtga gagagacaat agactcgacc
    25381 atatagtcaa caaaatgaat ccctttaatc ggttcccgga aacgagccag cgccctctcc
    25441 tggtcctgta agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc
    25501 gtcccgggac taatggcgca agatttacag atggctttca tcgaggcact gtgaaatcca
    25561 ttttctgaaa aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca
    25621 gtctgcatgt tatccatcat taaaccatta tagttagcgt gaataactag tttaccgcgt
    25681 tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa ggcacactct attcgtatac
    25741 ttttcactct gaaattggtt aaccaatagc cgtgatttgg ctgccagtta tttagcagag
    25801 acgttgatga gtttatcccg gtaaacaaca cgcacggttg tttaccggtc cgttagtgta
    25861 ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct
    25921 cgcagcaggc gagcaaagga acagcgctgc gttagcaata tcttgcggtt ggcccagacg
    25981 tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag
    26041 ggcatcggtt aatattgccc ccggcgcaat gccatttacc cgaatatttt tttcacccag
    26101 gtcaaacgcc atatttctga ccagatgact ggctgcagct ttagatgatg cataggaagt
    26161 catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc
    26221 atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag
    26281 ttcataagca cggcgaaaat ccgccattgg catatcaaac ggtttaggtc caccgccacc
    26341 ggcgttgtta accagaatat caactttacc cagcttactg atagcaaagt ctgccagtgc
    26401 agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgac cacccagttg
    26461 ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga
    26521 tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt
    26581 gatgatggcg catggcgcat ggcgcatggc gcatggcgca ttttccgtc
//
LOCUS       DAJTVE010000050        25763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000050 DAJTVE010000000
VERSION     DAJTVE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO439_001968"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO439_001968"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6316342.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="OO439_001969"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="OO439_001969"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6316343.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="OO439_001970"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="OO439_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6316344.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="OO439_001971"
     CDS             2440..3063
                     /locus_tag="OO439_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6316345.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO439_001972"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO439_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6316346.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="OO439_001973"
     CDS             complement(3447..3512)
                     /locus_tag="OO439_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316347.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO439_001974"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO439_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6316348.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="OO439_001975"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="OO439_001975"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6316349.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO439_001976"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO439_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6316350.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO439_001977"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO439_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6316351.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO439_001978"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO439_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6316352.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO439_001979"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO439_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6316353.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO439_001980"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO439_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6316354.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO439_001981"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO439_001981"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6316355.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="OO439_001982"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="OO439_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6316356.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="OO439_001983"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="OO439_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6316357.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="OO439_001984"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="OO439_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6316358.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO439_001985"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO439_001985"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6316359.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="OO439_001986"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="OO439_001986"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6316360.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="OO439_001987"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="OO439_001987"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6316361.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="OO439_001988"
     CDS             23127..23711
                     /locus_tag="OO439_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6316362.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="OO439_001989"
     CDS             23816..24556
                     /locus_tag="OO439_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6316363.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25763
                     /gene="htrE"
                     /locus_tag="OO439_001990"
                     /pseudo
     CDS             24591..25763
                     /gene="htrE"
                     /locus_tag="OO439_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6326 a         6981 c         6416 g         6040 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ata
//
LOCUS       DAJTVE010000051        25431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000051 DAJTVE010000000
VERSION     DAJTVE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OO439_001991"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OO439_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6316364.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO439_001992"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO439_001992"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6316365.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OO439_001993"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OO439_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OO439_001994"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OO439_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6316366.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="OO439_001995"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="OO439_001995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6316367.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="OO439_001996"
     CDS             complement(6490..6846)
                     /locus_tag="OO439_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6316368.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="OO439_001997"
     CDS             complement(6850..7266)
                     /locus_tag="OO439_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6316369.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OO439_001998"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OO439_001998"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6316370.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="OO439_001999"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="OO439_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OO439_002000"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OO439_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6316371.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OO439_002001"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OO439_002001"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6316372.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OO439_002002"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OO439_002002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6316373.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="OO439_002003"
     CDS             13294..14277
                     /locus_tag="OO439_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6316374.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OO439_002004"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OO439_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6316375.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OO439_002005"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OO439_002005"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6316376.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="OO439_002006"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="OO439_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="OO439_002007"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="OO439_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6316377.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="OO439_002008"
     CDS             complement(18079..18288)
                     /locus_tag="OO439_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6316378.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OO439_002009"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OO439_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6316379.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OO439_002010"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OO439_002010"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6316380.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OO439_002011"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OO439_002011"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6316381.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="OO439_002012"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="OO439_002012"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6316382.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OO439_002013"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OO439_002013"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6316383.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24521..25018)
                     /gene="ubiC"
                     /locus_tag="OO439_002014"
     CDS             complement(24521..25018)
                     /gene="ubiC"
                     /locus_tag="OO439_002014"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6316384.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25241..>25431)
                     /locus_tag="OO439_002015"
     CDS             complement(25241..>25431)
                     /locus_tag="OO439_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316385.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6581 a         6543 c         6088 g         6219 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcggat
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata acgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt tagtgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata ttttttcctc ttagtacaac ggtgacgccg
    24541 gtaaaaacag ttctgttagc aacagcggtt taccgcttaa tcgcaggcgg gaacgtcgcc
    24601 cccacagtcc ggcatcacgg cctatctcaa taaagtcccg ggttaatgtc gatgatgtga
    24661 acagatagcg tcctagcggc gttttaccca atttttgtaa cgccagctcc ggcccgctta
    24721 acgttgacac aggaacgacg gtacgaccgg caagccacgg ttcaccatcg gcacataaca
    24781 aaatttcacg taaccagtaa cgagactctt tcggcagcag cggcagttct tcggggattt
    24841 cattctgctc gacaaaccct tcgcggatca tcgtcacgct caccgttttt ccctgctgtt
    24901 caaaacgttt tgtcatggaa tcttccagca acagccagtc gagcagttgc gaatccaggg
    24961 cagggatctc tttaaaatag cgcagcgcac gcagttgcgt taacgcgggg tgtgacatgc
    25021 cgaactctcc gttacataac gtaaaggcat tgtatcgcag aaaagtgttt acgggagatg
    25081 taaagcaaat taagtgatct aatgcgcaac aaaaacgcaa caatcgcatg aagtggtaaa
    25141 acgctgttga accttacgct ttgccgatgc ccgtgctggc gagggcaacc gtaggggcat
    25201 caccgcccat gatgccagga acggtgatgt tttcctttac ttaaatacct atattcttta
    25261 tacgagtagc tcgccaactc gcagtaaaaa ttgggaaaag cgcatattta ccatgagatt
    25321 tggagctaaa aagtgtttcc tgagtaagat taataggcaa catcttatgt ctggatttat
    25381 agatacacgt ggaattccaa ttggtatctt ttgtaggatt caacatatca t
//
LOCUS       DAJTVE010000052        25343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000052 DAJTVE010000000
VERSION     DAJTVE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..334
                     /locus_tag="OO439_002016"
     CDS             <1..334
                     /locus_tag="OO439_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6316386.1"
                     /translation="FESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALH
                     ISTEPSAEWRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANIS
                     PETTVKGK"
     gene            336..755
                     /gene="gspB"
                     /locus_tag="OO439_002017"
     CDS             336..755
                     /gene="gspB"
                     /locus_tag="OO439_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6316387.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            993..1304
                     /gene="rpsJ"
                     /locus_tag="OO439_002018"
     CDS             993..1304
                     /gene="rpsJ"
                     /locus_tag="OO439_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6316388.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1337..1966
                     /gene="rplC"
                     /locus_tag="OO439_002019"
     CDS             1337..1966
                     /gene="rplC"
                     /locus_tag="OO439_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6316389.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1977..2582
                     /gene="rplD"
                     /locus_tag="OO439_002020"
     CDS             1977..2582
                     /gene="rplD"
                     /locus_tag="OO439_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6316390.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2579..2881
                     /gene="rplW"
                     /locus_tag="OO439_002021"
     CDS             2579..2881
                     /gene="rplW"
                     /locus_tag="OO439_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6316391.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2899..3720
                     /gene="rplB"
                     /locus_tag="OO439_002022"
     CDS             2899..3720
                     /gene="rplB"
                     /locus_tag="OO439_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6316392.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3737..4015
                     /gene="rpsS"
                     /locus_tag="OO439_002023"
     CDS             3737..4015
                     /gene="rpsS"
                     /locus_tag="OO439_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6316393.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4030..4362
                     /gene="rplV"
                     /locus_tag="OO439_002024"
     CDS             4030..4362
                     /gene="rplV"
                     /locus_tag="OO439_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6316394.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4380..5081
                     /gene="rpsC"
                     /locus_tag="OO439_002025"
     CDS             4380..5081
                     /gene="rpsC"
                     /locus_tag="OO439_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6316395.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5094..5504
                     /gene="rplP"
                     /locus_tag="OO439_002026"
     CDS             5094..5504
                     /gene="rplP"
                     /locus_tag="OO439_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6316396.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5504..5695
                     /gene="rpmC"
                     /locus_tag="OO439_002027"
     CDS             5504..5695
                     /gene="rpmC"
                     /locus_tag="OO439_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6316397.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5695..5949
                     /gene="rpsQ"
                     /locus_tag="OO439_002028"
     CDS             5695..5949
                     /gene="rpsQ"
                     /locus_tag="OO439_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6316398.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6114..6485
                     /gene="rplN"
                     /locus_tag="OO439_002029"
     CDS             6114..6485
                     /gene="rplN"
                     /locus_tag="OO439_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6316399.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6496..6810
                     /gene="rplX"
                     /locus_tag="OO439_002030"
     CDS             6496..6810
                     /gene="rplX"
                     /locus_tag="OO439_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6316400.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6825..7364
                     /gene="rplE"
                     /locus_tag="OO439_002031"
     CDS             6825..7364
                     /gene="rplE"
                     /locus_tag="OO439_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6316401.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7379..7684
                     /gene="rpsN"
                     /locus_tag="OO439_002032"
     CDS             7379..7684
                     /gene="rpsN"
                     /locus_tag="OO439_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6316402.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7718..8110
                     /gene="rpsH"
                     /locus_tag="OO439_002033"
     CDS             7718..8110
                     /gene="rpsH"
                     /locus_tag="OO439_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6316403.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8123..8656
                     /gene="rplF"
                     /locus_tag="OO439_002034"
     CDS             8123..8656
                     /gene="rplF"
                     /locus_tag="OO439_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6316404.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8666..9019
                     /gene="rplR"
                     /locus_tag="OO439_002035"
     CDS             8666..9019
                     /gene="rplR"
                     /locus_tag="OO439_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6316405.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9034..9537
                     /gene="rpsE"
                     /locus_tag="OO439_002036"
     CDS             9034..9537
                     /gene="rpsE"
                     /locus_tag="OO439_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6316406.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9541..9720
                     /gene="rpmD"
                     /locus_tag="OO439_002037"
     CDS             9541..9720
                     /gene="rpmD"
                     /locus_tag="OO439_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6316407.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9724..10158
                     /gene="rplO"
                     /locus_tag="OO439_002038"
     CDS             9724..10158
                     /gene="rplO"
                     /locus_tag="OO439_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6316408.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10166..11497
                     /gene="secY"
                     /locus_tag="OO439_002039"
     CDS             10166..11497
                     /gene="secY"
                     /locus_tag="OO439_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6316409.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11529..11645
                     /gene="rpmJ"
                     /locus_tag="OO439_002040"
     CDS             11529..11645
                     /gene="rpmJ"
                     /locus_tag="OO439_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6316410.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11792..12148
                     /gene="rpsM"
                     /locus_tag="OO439_002041"
     CDS             11792..12148
                     /gene="rpsM"
                     /locus_tag="OO439_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6316411.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12165..12554
                     /gene="rpsK"
                     /locus_tag="OO439_002042"
     CDS             12165..12554
                     /gene="rpsK"
                     /locus_tag="OO439_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6316412.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12588..13208
                     /gene="rpsD"
                     /locus_tag="OO439_002043"
     CDS             12588..13208
                     /gene="rpsD"
                     /locus_tag="OO439_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6316413.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13234..14223
                     /gene="rpoA"
                     /locus_tag="OO439_002044"
     CDS             13234..14223
                     /gene="rpoA"
                     /locus_tag="OO439_002044"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6316414.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14264..14647
                     /gene="rplQ"
                     /locus_tag="OO439_002045"
     CDS             14264..14647
                     /gene="rplQ"
                     /locus_tag="OO439_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6316415.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14754..15122
                     /locus_tag="OO439_002046"
     CDS             14754..15122
                     /locus_tag="OO439_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6316416.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15133..15558
                     /gene="zntR"
                     /locus_tag="OO439_002047"
     CDS             15133..15558
                     /gene="zntR"
                     /locus_tag="OO439_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6316417.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15614..15832
                     /gene="arfA"
                     /locus_tag="OO439_002048"
     CDS             15614..15832
                     /gene="arfA"
                     /locus_tag="OO439_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6316418.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15829..16239)
                     /gene="mscL"
                     /locus_tag="OO439_002049"
     CDS             complement(15829..16239)
                     /gene="mscL"
                     /locus_tag="OO439_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6316419.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16369..17745)
                     /gene="trkA"
                     /locus_tag="OO439_002050"
     CDS             complement(16369..17745)
                     /gene="trkA"
                     /locus_tag="OO439_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6316420.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17767..19056)
                     /gene="rsmB"
                     /locus_tag="OO439_002051"
     CDS             complement(17767..19056)
                     /gene="rsmB"
                     /locus_tag="OO439_002051"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6316421.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19102..20049)
                     /gene="fmt"
                     /locus_tag="OO439_002052"
     CDS             complement(19102..20049)
                     /gene="fmt"
                     /locus_tag="OO439_002052"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6316422.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20064..20573)
                     /gene="def"
                     /locus_tag="OO439_002053"
     CDS             complement(20064..20573)
                     /gene="def"
                     /locus_tag="OO439_002053"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6316423.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20703..21827
                     /gene="dprA"
                     /locus_tag="OO439_002054"
     CDS             20703..21827
                     /gene="dprA"
                     /locus_tag="OO439_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6316424.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21799..22272
                     /locus_tag="OO439_002055"
     CDS             21799..22272
                     /locus_tag="OO439_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6316425.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22301..22843
                     /locus_tag="OO439_002056"
     CDS             22301..22843
                     /locus_tag="OO439_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6316426.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22848..23420
                     /gene="tsaC"
                     /locus_tag="OO439_002057"
     CDS             22848..23420
                     /gene="tsaC"
                     /locus_tag="OO439_002057"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6316427.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23425..24243
                     /gene="aroE"
                     /locus_tag="OO439_002058"
     CDS             23425..24243
                     /gene="aroE"
                     /locus_tag="OO439_002058"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6316428.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24240..24497
                     /locus_tag="OO439_002059"
     CDS             24240..24497
                     /locus_tag="OO439_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6316429.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24473..25027)
                     /locus_tag="OO439_002060"
     CDS             complement(24473..25027)
                     /locus_tag="OO439_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6316430.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6455 a         5990 c         6637 g         6261 t
ORIGIN      
        1 gtttgagtcg gtatggacgg gtgattatct tctgttgtgg aaaatgtcgc cggaagggga
       61 gagtaccatt acgcgtgata gcagcgagga agagattctc tggctggaaa cgatgttaaa
      121 ccgtgcattg catatttcga cagaaccttc ggcggagtgg cgtcccttgt tggtagaaaa
      181 aataaagcaa tttcaaaaaa gccaccactt gaaaactgac ggtgttgttg gcttttcaac
      241 gttagtacat ctctggcagg tggcagggga gagtgcctac ttatatcggg atgaagcaaa
      301 tatttccccg gaaacaacag tgaaggggaa atgacatgtt tgaattctat atcgccgcgc
      361 gtgagcagaa agaaacaggt caccctggta tcttctcccg acaaaaacac agcaccataa
      421 tatatgtcat ttgtttattg ctgatttgcc tgtggtttgc aggaatggtg ttggttggtg
      481 ggtatgccag gcagctatgg gcgctttgga tagtaaaagc agaagtcact gtagaggctg
      541 aaacgcccgc ttttaaacaa tcaacacaac actatttctt caaaaaacag ccactccccg
      601 tcgtcgaatc cgtcgaggag gaagatgacc cagacgtcgc ggttgagaat gctccctctt
      661 cttctgaaga cgaagaaaat actgtaaaag aaagcgacga gaaagccggt ttaagagagc
      721 gtgtcaaaaa tgcgctgaac gaacttgaga gataacccga aggctgttta ctttactagc
      781 aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg ggcttgtcgt agttgacagc
      841 aggttcaatc tgaacctcag ttagccgaat ttggctacct aacaatgctc ccaatcgggg
      901 agctacgtaa gaacggttac actctcccat caatcgtaat gggtctgagg agtaatcatt
      961 ttcgtttata aaataattgg agctctggtc tcatgcagaa ccaaagaatc cgtatccgcc
     1021 tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc ggaaatcgtc gagactgcca
     1081 agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc gacacgcaaa gagcgcttca
     1141 ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga tcagtacgaa atccgtactc
     1201 acttgcgtct ggttgacatc gttgagccaa ccgagaaaac cgttgatgct ctgatgcgtc
     1261 tggatctggc tgccggtgta gacgtgcaga tcagcctggg ttaatcaggt cattgagcga
     1321 ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa gtgggtatga cccgtatctt
     1381 cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa gttgaagcaa accgcgttac
     1441 tcaggttaaa gacctggcta acgatggcta ccgtgctatt caggtgacca ccggtgctaa
     1501 aaaagctaac cgtgtgacca agcctgaagc tggccacttc gctaaagctg gcgtagaagc
     1561 tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa gagttcactg taggtcagag
     1621 cattagcgtt gaactgtttg ctgaggttaa aaaagttgac gtaactggca cctctaaagg
     1681 taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt acccaggacg ctactcacgg
     1741 taactccttg tctcaccgcg ttccgggttc tatcggtcag aaccagactc cgggcaaagt
     1801 gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa cgtgtaaccg ttcagagcct
     1861 tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg gttaaaggtg ctgtcccggg
     1921 tgcaaccggt agcgacctga tcgttaaacc agctgtgaag gcgtaaggag atagcaatgg
     1981 aattagtatt gaaagacgcg cagagcgcgc tgactgtttc cgaaactacc ttcggtcgtg
     2041 atttcaacga agcgctggtt caccaggttg ttgttgctta tgcagctggt gctcgtcagg
     2101 gtactcgtgc tcagaagact cgtgctgaag taactggttc cggtaaaaaa ccgtggcgcc
     2161 agaaaggcac cggccgtgcg cgttctggtt ctatcaagag cccgatctgg cgttctggtg
     2221 gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa agttaacaag aagatgtacc
     2281 gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca ggatcgtctg atcgttgtcg
     2341 agaagttctc tgtagaagcg ccgaaaacta agctgctggc acagaaactg aaagacatgg
     2401 ctctggaaga tgtgctgatc atcaccggtg agctggacga aaacctgttc ctggctgcgc
     2461 gcaacctgca caaggttgac gtacgcgatg caactggtat cgacccggtt agcctgatcg
     2521 ccttcgacaa agtcgtaatg actgctgatg ctgttaagca agttgaggag atgctggcat
     2581 gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg cacgtttctg aaaaagcgtc
     2641 tactgcgatg gaaaaatcca acaccatcgt actcaaagtt gctaaagacg cgaccaaagc
     2701 agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa gtcgaagtcg ttaacaccct
     2761 ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc ggtcgtcgta gcgactggaa
     2821 aaaagcttac gtcaccctga aagaaggcca gaatctggac ttcgttggcg gcgctgagta
     2881 agtcggagga gtaatacaat ggcagttgtt aaatgtaaac cgacatctcc gggtcgtcgc
     2941 cacgtagtta aagtggttaa ccctgagctg cacaagggca aaccttttgc tccgttgctg
     3001 gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc gtatcaccac tcgtcatatc
     3061 ggtggtggcc acaagcaggc ttaccgtatt gttgacttca aacgcaacaa agacggtatc
     3121 ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt ccgcgaacat cgcgctggtt
     3181 ctgtacaaag acggtgaacg ccgttacatc ctggccccta aaggcctgaa agctggcgac
     3241 cagattcagt ctggcgttga tgctgcaatc aaaccaggta acaccctgcc gatgcgcaac
     3301 atcccggttg gttctactgt tcataacgta gaaatgaaac caggtaaagg cggtcagctg
     3361 gcacgttccg ctggtactta cgttcagatc gttgctcgtg atggtgctta tgtcaccctg
     3421 cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact gccgtgcaac tctgggcgaa
     3481 gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag caggtgctgc acgctggcgt
     3541 ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg tagaccaccc acatggtggt
     3601 ggtgaaggtc gtaactttgg taagcacccg gtaactccgt ggggcgttca gaccaaaggt
     3661 aagaagaccc gcagcaacaa gcgtactgat aaattcatcg tacgtcgccg tagcaaataa
     3721 ttttagagga taagccatgc cacgttctct caagaaaggt ccttttattg acctgcactt
     3781 gctgaagaag gtagagaaag cggtggaaag cggagacaag aagcccctgc gcacttggtc
     3841 ccgtcgttca acgatctttc ctaacatgat cggtttgacc atcgctgtcc ataatggtcg
     3901 tcagcacgtt ccggtatttg tgaccgacga aatggtcggt cacaaactgg gtgaattcgc
     3961 accgactcgt acttatcgcg gccacgctgc tgataaaaaa gcgaagaaga aataaggtag
     4021 gaggaagaga tggaaactat cgctaaacat cgccatgctc gttcttctgc tcagaaggtt
     4081 cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc aggctctgga tattttgacc
     4141 tacaccaaca agaaagcggc tgtactggtc aagaaagttc tggaatctgc cattgctaac
     4201 gctgaacaca acgatggcgc tgacattgac gatctgaaag ttacgaaaat tttcgtagac
     4261 gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag gtcgtgcaga tcgcatcctg
     4321 aagcgcacca gccacatcac tgtggttgtg tccgatcgct gagactctgg agactagcaa
     4381 tgggtcagaa agtacatcct aatggtattc gcttgggtat tgtaaaacca tggaactcta
     4441 cctggtttgc gaacaccaaa gaattcgctg acaacctgga cagcgatttt aaagtacgtc
     4501 agtacctgac taaggaactg gctaaagcgt ctgtatctcg tatcgttatc gagcgtccgg
     4561 ctaagagcat ccgtgtaacc attcacactg ctcgcccggg tatcgttatc ggtaaaaaag
     4621 gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat cgctggcgtt cctgcacaga
     4681 tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa actggttgct gacagcatca
     4741 cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat gaagcgtgct gtacagaacg
     4801 caatgcgtct gggcgctaaa ggtattaaag ttgaagttag cggccgtctg ggcggcgcgg
     4861 aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc gctgcacact ctgcgtgctg
     4921 acatcgacta caacacctct gaagcgcaca ccacttacgg tgtaatcggc gttaaagtgt
     4981 ggatcttcaa aggcgagatc ctgggtggta tggctgctgt tgaacaaccg gaaaaaccgg
     5041 ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata aggagcgtcg ctgatgttac
     5101 aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg taaccgcggt ctggcgcagg
     5161 gtacggatgt tagcttcggc agcttcggtc tgaaagctgt tggccgtggt cgtctgactg
     5221 cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc agttaagcgt caaggtaaga
     5281 tctggatccg tgtgttcccg gacaaaccga tcactgaaaa gccgctggca gtgcgtatgg
     5341 gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat tcagccgggt aaagtcctgt
     5401 atgaaatgga cggtgttccg gaagagctgg cccgtgaagc attcaagctg gcagcagcga
     5461 aactgccgat taaaaccacc tttgtaacta agacggtgat gtaatgaaag caaaagagct
     5521 gcgtgagaag agcgttgaag agctgaacac cgagctgctg aacctgctgc gtgagcagtt
     5581 caacctgcgt atgcaggctg caagtggcca gctgcaacag tctcacctgt tgaagcaagt
     5641 gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag aaggcgggtg cgtaatgacc
     5701 gataaaatcc gtactctgca aggtcgcgtt gttagcgaca aaatggagaa atccattgtt
     5761 gttgctatcg aacgttttgt gaaacacccg atctacggta aattcatcaa gcgtacgacc
     5821 aaactgcacg tacatgacga gaacaacgaa tgcggtatcg gtgacgtggt tgaaatccgc
     5881 gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg ttcgcgttgt agagaaagcg
     5941 gttctgtaat acagtacact ctctcgatac gaataaacgg ctcagaaatg agccgtttat
     6001 tttttctacc catatccttg aagcggtgtt ataatgccgc gccctcgata tggggatttt
     6061 taacgacctg attttcgggt ctcagtagta gttgacatta gcggagcact aaaatgatcc
     6121 aagaacagac tatgctgaac gtcgccgaca actccggtgc acgtcgcgta atgtgtatca
     6181 aggttctggg tggctcgcac cgtcgctacg caggcgtagg cgacatcatc aagatcacca
     6241 tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga tgtgctgaag gcggtagtgg
     6301 tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt cattcgcttc gatggtaatg
     6361 cttgtgttct tctgaacaac aacagcgagc agcctatcgg tacgcgtatt tttgggccgg
     6421 taactcgtga gcttcgtagt gagaagttca tgaaaattat ctctctggca ccagaagtac
     6481 tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg acgaagttat cgtgttaacc
     6541 ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc tgtcttccgg caaggtcatt
     6601 gttgaaggta tcaacctggt taagaaacat cagaagccgg ttccggccct gaaccaaccg
     6661 ggtggcatcg ttgaaaaaga agccgctatt caggtttcca acgtagcaat cttcaatgcg
     6721 gcaaccggca aggctgaccg tgtaggcttt agattcgaag acggcaaaaa agtccgtttc
     6781 ttcaagtcta acagcgaaac tatcaagtaa tttggagtag tacgatggcg aaactgcatg
     6841 attactacaa agacgaagta gttaaaaaac tcatgactga gtttaactac aattctgtca
     6901 tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg tgttggtgaa gcgatcgctg
     6961 acaaaaaact gctggataac gcagcagcag acctggcagc aatctccggt caaaaaccgc
     7021 tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat ccgtcagggc tatccgatcg
     7081 gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt ctttgagcgc ctgatcacta
     7141 ttgctgtacc tcgtattcgt gacttccgtg gcctgtccgc taagtctttc gacggtcgtg
     7201 gtaactacag catgggtgtc cgtgagcaga tcatcttccc agaaatcgac tacgataaag
     7261 tcgaccgcgt tcgtggtttg gatattacca ttaccactac tgcgaaatct gacgaagaag
     7321 gccgcgctct gctggctgcc tttgacttcc cgttccgcaa gtaaggtagg gttactaaat
     7381 ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta gctttagctg ataaatactt
     7441 cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg aacgcttccg acgaagatcg
     7501 ttggaacgct gttctcaagc tgcagactct gccgcgtgat tccagcccgt ctcgtcagcg
     7561 taaccgctgc cgtcaaacag gtcgcccgca tggtttcctg cggaagttcg ggttgagccg
     7621 tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg ggtctgaaaa aggctagctg
     7681 gtaattgtca ccaattgaat cacgggagta aagacagatg agcatgcaag atccgatcgc
     7741 ggatatgctg acccgtatcc gtaacggtca ggccgcgaac aaagctgcgg tcaccatgcc
     7801 ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag gaagaaggtt ttattgaaga
     7861 ttttaaagtt gaaggcgaca ccaagcctga actggaactt actctgaagt atttccaggg
     7921 caaagctgtt gtagaaagca ttcagcgtgt cagccgccca ggtctgcgca tctataaacg
     7981 taaagatgag ctgccgaaag ttatggcggg tctgggtatc gcagttgttt ctacctctaa
     8041 aggtgttatg actgatcgtg cagcgcgcca ggctggtctt ggtggcgaaa ttatctgcta
     8101 cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa gcaccggtcg ttgttcctgc
     8161 cggcgttgac gtaaaaatca acggtcaggt tattacgatc aaaggtaaaa acggcgagct
     8221 gactcgtact ctcaacgatg ctgttgaagt taaacatgca gataataccc tgaccttcgg
     8281 tccgcgtgat ggttacgcag acggttgggc acaggctggt accgcgcgtg ccctgctgaa
     8341 ctcaatggtt atcggtgtta ccgaaggctt cactaagaag ctgcagctgg ttggtgtagg
     8401 ttaccgtgca gcggttaaag gcaatgtgat taacctgtct ctgggtttct ctcatcctgt
     8461 tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg actcagactg aaatcgtgct
     8521 gaaaggcgtt gataagcagg tgatcggcca ggttgcagcg gatctgcgcg cctaccgtcg
     8581 tcctgagcct tataaaggca agggtgttcg ttacgccgac gaagtcgtgc gtaccaaaga
     8641 ggctaagaag aagtaaggta acactatgga taagaaattt gctcgtatcc gtcgtgcgac
     8701 ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc ctggtggtac atcgtacccc
     8761 gcgtcacatt tacgcacagg taattgcacc gaacggttct gaagttctgg tagctgcttc
     8821 tactgtagaa aaagctatcg ctgaacaact gaagtacacc ggtaacaaag acgcggctgc
     8881 agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa ggcatcaaag atgtatcctt
     8941 tgaccgttcc gggttccaat atcatggtcg tgtccaggca ctggcagatg ctgcccgtga
     9001 agctggcctt cagttctaag gtagaggtgt aagatggctc acatcgaaaa acaagctggc
     9061 gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta aaaccgttaa aggtggtcgt
     9121 attttctcct tcacagctct gactgtagtt ggcgatggta acggtcgcgt tggttttggt
     9181 tacggtaaag cgcgtgaagt tccagcagcg atccagaaag cgatggaaaa agcccgtcgc
     9241 aatatgatta acgtcgcgct gaataacggc actctgcaac accctgttaa aggtgttcac
     9301 acgggttctc gcgtattcat gcagccggct tccgaaggta ccggtatcat cgccggtggt
     9361 gcaatgcgcg ccgttctgga agtcgctggg gttcataacg ttctggctaa agcatatggt
     9421 tccaccaacc cgatcaacgt ggttcgtgca actattgatg gcctggaaaa tatgaattct
     9481 ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag aaattctggg gaaataaacc
     9541 atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa tcggtcgtct gccgaaacac
     9601 aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc acaccgtaga gcgcgaggat
     9661 actcctgcta ttcgcggtat gatcaacgcg gtttccttca tggttaaagt tgaggagtaa
     9721 gagatgcgtt taaatactct gtctccggcc gaaggctcca aaaaggcggg taaacgcttg
     9781 ggtcgtggta tcggttctgg cctcggtaaa accggtggtc gtggtcacaa aggtcagaag
     9841 tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg gtcagatgcc tctgtaccgt
     9901 cgtctgccga aattcggctt cacttctcgt aaagcagcga ttacagccga agttcgtctg
     9961 tctgacctgg ctaaagtaga aggcggtgtt gtagacctga acacgctgaa agcggctaac
    10021 attatcggta tccagatcga gttcgcgaaa gtgatcctgg ctggcgaagt aacgactccg
    10081 gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg ctgctatcga agctgctggc
    10141 ggtaaaatcg aggaataagt agcagatggc taaacaaccg ggattagatt ttcaaagtgc
    10201 caaaggtggc ttaggcgagc tgaaacgcag actgctgttt gttatcggtg cgctgattgt
    10261 gttccgtatt ggctctttta ttccgatccc tggtattgat gccgctgtac ttgccaaact
    10321 gcttgagcaa cagcgaggca ccatcattga gatgtttaac atgttctctg gtggtgctct
    10381 cagccgtgct tctatctttg ctctggggat catgccgtat atttcggcgt cgatcattat
    10441 ccagctgctg acggtggttc acccaacgtt ggcggaaatt aagaaagaag gggagtctgg
    10501 tcgtcgtaag atcagccagt acacccgcta cggtactctg gtgctggcaa tattccagtc
    10561 gatcggtatt gctactggtc tgccgaatat gcctggtatg caaggcctgg tgattaaccc
    10621 gggctttgca ttctacttca ccgctgttgt aagtctggtc acaggaacga tgttcctgat
    10681 gtggttgggc gaacagatta ctgaacgagg tatcggcaac ggtatttcaa tcattatctt
    10741 cgtcggtatt gtcgcgggac tcccgccagc cattgcccat actatcgagc aagcgcgtca
    10801 aggcgacctg cacttcctcg tgttgctgtt ggttgcagta ttagtatttg cagtgacgtt
    10861 ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg gtaaactacg cgaaacgtca
    10921 gcaaggtcgt cgtgtttatg ctgcacagag cacacattta ccgctgaaag tgaatatggc
    10981 gggggtaatc ccggcaatct tcgcttccag tattattctg ttcccggcga ccatcgcgtc
    11041 atggttcggg ggcggtactg gttggaactg gctgacaaca atttcgctgt atttgcagcc
    11101 tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc atcttcttct gtttcttcta
    11161 cacggcgttg gttttcaacc cgcgtgaaac agcagataac ctgaagaagt ccggtgcatt
    11221 tgtaccagga attcgtccgg gagagcaaac ggcgaagtat atcgataaag taatgacccg
    11281 cctgaccctg gttggtgcgc tgtatattac ctttatctgc ctgatcccgg agttcatgcg
    11341 tgatgcaatg aaagtaccgt tctacttcgg tgggacctca ctgcttatcg ttgttgtcgt
    11401 gattatggac tttatggctc aagtgcaaac tctgatgatg tccagtcagt atgagtctgc
    11461 attgaagaag gcgaacctga aaggctacgg ccgataattg gtcgcccgag aagttacgga
    11521 gagtaaaaat gaaagttcgt gcttccgtca agaaattatg ccgtaactgc aaaatcgtta
    11581 agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc gaagcataaa cagcgccaag
    11641 gctgattttt tcgcatattt ttcttgcaaa gttgggttga gctggctaga ttagccagcc
    11701 aatcttttgt atgtctgtgc gtttccattt gagtatcctg aaaacgggct tttcagcatg
    11761 gaacgtacat attaaatagt aggagtgcat agtggcccgt atagcaggca ttaacattcc
    11821 tgatcataag catgccgtaa tcgcattaac ttcgatttat ggcgtcggca agacccgttc
    11881 taaagccatc ctggctgcag cgggtatcgc tgaagatgtt aagatcagtg agctgtctga
    11941 aggacaaatc gacacgctgc gtgacgaagt tgccaaattt gtcgttgaag gtgatctgcg
    12001 ccgtgaaatc agcatgagca tcaagcgcct gatggatctt ggttgctatc gcggtttgcg
    12061 tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag accaacgcac gtacccgtaa
    12121 gggtccgcgc aaaccgatca agaaataatc ggggtgattg aataatggca aaggcaccaa
    12181 ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg cgtggctcat atccatgctt
    12241 ctttcaacaa caccatcgtg actatcactg atcgtcaggg taacgcgttg ggttgggcaa
    12301 cagccggtgg ttccggtttc cgtggttctc gcaaatccac tccgtttgca gctcaggttg
    12361 cagcagagcg ttgcgctgac gccgtgaaag aatacggcat caagaatctg gaagttatgg
    12421 ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc tctgaacgcc gcaggtttcc
    12481 gcatcactaa cattactgat gtgactccga tccctcataa cggttgtcgt ccgccgaaaa
    12541 aacgtcgcgt ataacgcctc gttttccagg tttgttggag aaagaaaatg gcaagatatt
    12601 tgggtcctaa gctcaagctg agccgtcgtg agggcaccga cttattcctt aagtctggcg
    12661 ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc tggccagcac ggtgcgcgta
    12721 aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa gcaaaaagtt cgccgtatct
    12781 atggtgtgct ggagcgtcag ttccgtaact actacaaaga agcagcacgt ctgaaaggca
    12841 acaccggtga aaacctgttg gctctgctgg aaggtcgtct ggacaacgtt gtataccgta
    12901 tgggcttcgg tgccactcgt gcagaagcac gtcagctggt tagccataaa gcaattatgg
    12961 taaacggtcg tgttgttaac atcgcttctt atcaggttag tccgaatgac gttgtaagca
    13021 ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc tctggagctg gctgagcagc
    13081 gtgaaaagcc aacttggctg gaagttgatg ctggcaagat ggaaggtacg tttaagcgta
    13141 agccggagcg ttctgatctg tctgcggaca ttaacgaaca cctgatcgtc gagctttact
    13201 ccaagtaaag cttagtacca aagagaggac acaatgcagg gttctgtgac agagtttcta
    13261 aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc acgccaaggt gacccttgag
    13321 cctttagagc gtggctttgg ccatactctg ggtaacgcac tgcgccgtat tctgctctca
    13381 tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg gtgtactaca tgagtacagc
    13441 accaaagaag gcgttcagga agatatcctg gaaatcctgc tcaacctgaa agggctggcg
    13501 gtgagagttc agggcaaaga tgaagttatt cttaccttga ataaatctgg cattggccct
    13561 gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa tcgtcaagcc gcagcacgtg
    13621 atctgccacc tgaccgatga gaacgcgtct attagcatgc gtatcaaagt tcagcgcggt
    13681 cgtggttatg tgccggcttc tacccgaatt cattcggaag aagatgagcg cccaatcggc
    13741 cgtctgctgg tcgacgcatg ctacagccct gtggagcgta ttgcctacaa tgttgaagca
    13801 gcgcgtgtag aacagcgtac cgacctggac aagctggtca tcgaaatgga aaccaacggc
    13861 acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca ttctggctga acaactggaa
    13921 gctttcgttg acttacgtga tgtacgtcag cctgaagtga aagaagagaa accagagttc
    13981 gatccgatcc tgctgcgccc tgttgacgat ctggaattga ctgtccgctc tgctaactgc
    14041 cttaaagcag aagctatcca ctatatcggt gatctggtac agcgtaccga ggttgagctc
    14101 cttaaaacgc ctaaccttgg taaaaaatct cttactgaga ttaaagacgt gctggcttcc
    14161 cgtggactgt ctctgggcat gcgcctggaa aactggccac cggcaagcat cgctgacgag
    14221 taaccggatc acaggttaag gttttactga gaaggataag gtcatgcgcc atcgtaagag
    14281 tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct atgttccgca atatggcagg
    14341 ttcactggtt cgtcatgaaa tcatcaagac gactctgcct aaagcgaaag agctgcgccg
    14401 cgtagttgag ccgctgatta ctcttgccaa gactgatagc gttgctaatc gtcgtctggc
    14461 atttgcccgt actcgtgata acgagatcgt ggcaaaactg tttaacgaac tgggcccgcg
    14521 tttcgcgagc cgtgccggtg gttacactcg tattctgaag tgtggcttcc gtgcaggcga
    14581 caacgcgccg atggcttaca tcgagctggt tgatcgttca gagaaagcag aagctgctgc
    14641 agagtaatct gaagcaatgt aaaaaaaccc gccccggcgg gtttttttat acccgtagta
    14701 tccccactta tctacaatag ctgtactctt tttgttcatc ccctggagta tttatgtggt
    14761 tacttgacca gtgggcagag cgccatatag cagaagcgca agcgaaaggt gagtttgata
    14821 acctggcagg tagcggcgaa ccattgatac tggatgatga ttctcacgtg ccaccggaat
    14881 tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt accgccagaa cttgagcaac
    14941 ggagagaagc aattcagctt ctggatattc tcaaaggtat ccgtcacgat gatccgcaat
    15001 atcaagaggt tagccgtcga ttgtcattac tggaattgaa gctgcgacaa gctggattga
    15061 gtaccgattt tttacgcggc gattatgctg acaagttgtt ggacaaaatc aacgataact
    15121 agtggagtat gtatgtatcg cattggtgag ctggcaaaaa tggcggaagt aacacctgac
    15181 acgattcgtt attacgaaaa acagcagatg atggagcatg aagtgcgtac tgaaggcggg
    15241 tttcgcctgt ataccgaaag cgatctccag cgattgaaat ttatccgcca tgccagacaa
    15301 ctaggtttca gtctggagtc gatccgcgag ttgctgtcga tccgcatcga tcctgaacac
    15361 catacctgtc aggagtcaaa aggcattgtg caggaaagat tgcaggaagt cgaagcacgg
    15421 atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc ttaacgatgc ctgttgtggg
    15481 accgcccata gcagtgttta ttgctcgatt cttgaagctc ttgaacaagg ggcgagtggc
    15541 gttaagagtg gctgttgatt ttttgcactg acaggattac actcgcgccg ttaaataacc
    15601 aactggagtt tttatgagcc gatatcagca tactaaaggg cagataaagg ataatgcgat
    15661 agaagcatta ctacatgatc ccttattccg acagcgagta gagaaaaata agaaagggaa
    15721 aggcagttac atgcgaaaag gaaaacatgg caatcggggg aactgggagg ccagtggcaa
    15781 gaaagtaaat cactttttta ccactggtct tctgctttca ggcgcttgtt aagagcggtt
    15841 attctgctct ttcagcaaat cacgaatttc tgccagtaat acttcttctt tagttggtgc
    15901 aggtgcggca gctggttctt cttttttccg attcagtttg ttgattagct taatcgccat
    15961 aaagatggca aaggccacaa tcagaaaatc aaagacgttt tgaatgaaga caccgtaatg
    16021 catcacaaca gcagggatat ccccctgcgc atcgcgtagc gtgacagcaa actgtttaaa
    16081 gtcgatcccg ccaattaata agcccagagg tggcatgatg atatcggcaa ccagtgaaga
    16141 tacaatcttc ccgaatgccg caccgataat gacacccacc gccaaatcca ccacgttccc
    16201 gcgcatcgca aattcgcgaa attctttaat aatgctcatg ttattctccc tatgaagccg
    16261 acaaccataa gtctaacaaa tgttaagcca ttttcctgcc aggaatgaat ttaattaatc
    16321 cttgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt acaagaagaa
    16381 agggcttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg tgaggaacat
    16441 aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca cgtcgtttcc
    16501 acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcgtcaa tgactctgcc
    16561 gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt ctgctacgcc
    16621 gcggcgcaat gaggaaacac caacaatatc tgcttttcgc acatggctaa gcaacgcaga
    16681 aatagttgct tgttgtggtg aaattgcaat atcgatcacg ctcccctgaa ccagatccac
    16741 ataagcgcga cgctggatca ataccatcac ctttttcgca cccatacgtt tggcaagcat
    16801 ggcggacatg atattggcct cgtcatcgtt ggtgacggca ataaacagat caacttgatc
    16861 gatatgttct tcggccagca gttcttgatc cgacgcatca ccaaaaaaga cgatcgtatt
    16921 ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga gtttaacgct
    16981 gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccac caaccagcat
    17041 gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcgc ggatatgctg
    17101 tgaagcggca ataaagaaca cttcatcacc agcttcaaca atggtcgaac cttgtggacg
    17161 aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatgtg gcatatgttc
    17221 gcgcatggtc gaaagtgcat taccaatcag cgggccgcca taataggctt taactacagc
    17281 gaggctgact ttaccctcag cgaagttcac cacctgcaat gcgcctggat actcaatcag
    17341 tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa tcggtacagc
    17401 atctgaatga aatagcttat cggcatcgcg tacgtagtct ggtgagcgga tgcgagcgat
    17461 gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat tggtttcatc
    17521 tgaactggtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc gcaatacgcg
    17581 tggatgagag ccatgcccct gcacgactcg caggtcaaat ttatcctgca aggtccgcag
    17641 acgctcaccg ttggtatcga caacagtaat atcgttgttc tcgccaacca ggttttccgc
    17701 cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc tcgacccgtt
    17761 atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt cggcaccagg
    17821 cagattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg catcagcggt
    17881 acgttgcaaa aaagctttaa tctgcaggct attctcttcc ggtaacaccg aacaggtggc
    17941 atagaccagg gttccaccgg attttaaatg cggccaaatg gcgtcgagaa tttcagactg
    18001 caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat ccggatggcg
    18061 acgaatcacc cccgttgctg aacaaggcgc atctaataaa atgcgatcaa actgttgctc
    18121 gccacaccat tgggaagggt aacggccatc accttgtttc acggtcgcct tcataccaag
    18181 gcgttttaaa ttgtcgtgaa cgcgagagag gcgctgttcg tcaatatcaa ccgcaacaac
    18241 ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg cggcacaaag
    18301 atccagaatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt gtgctgatgc
    18361 atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag gtgcaggtgt
    18421 ttccagccgt acagcatcag ggtaatccgc atgcgggaaa cctttcattc ctgcttcatc
    18481 caacaatgca agccagctgt cgcgggaatg atgcgtacga ttaacgcgca gccacattgg
    18541 cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg ctttttgcag
    18601 acgcttcagc aaccaggaag gatgcagata acgtgcatca ctggtattaa actcggctaa
    18661 taactcttct tgctgacgct ggaactggcg taatacaccg ttaatcaacc ctttaagttg
    18721 cggacgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat gaggtggaat
    18781 gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacag tgcgctgttt
    18841 gccggtcatc ggacgggcca ttaacttatt aatcagccag tctaactgcg aaagcgtacg
    18901 cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcagaaa ctttttgctg
    18961 gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt caacggcctg
    19021 ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaatgc ccggtaagac
    19081 cgggcttaga agagtggact atcaggccag acggttgccc ggaacaaacc attcccgacg
    19141 agaattcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta acgagagcag
    19201 gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttgttggctt caaggatcgt
    19261 tcctggtgca gcgttggttg ccgtatcaat gaccgacgct ttccagactt taaccggttg
    19321 tccttcaatt tccagccagc tcattggcca tgggttgaaa gcgcgaatgc agcgttcaag
    19381 ctgcgctgcc gaaagcgacc agtcaatacg cgcttcttct ttactcaact tctcggcgta
    19441 agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcta gttgtttcaa
    19501 cgtggtgatt agcccttgtg ggccaagctc tgccagcttg tcgtacagcg taccactggt
    19561 atcttctgca gtaatcgggc aggagagctt atagagcata tcaccggtgt ctaaaccgac
    19621 atccatttgc ataatggtca caccagtttc tgcatcaccc gcccatagtg agcgttggat
    19681 tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac agccaagacg
    19741 cggcatctcc agcactgctt taggcagaat taagccatag gcgacgacga ccataacatc
    19801 agcctgcaga tcggcgacca gttgctggct ttcttgtgga cgcagggaaa caggttgaaa
    19861 aacgggcaga cctttttcct cagccagaac tttaaccggg ctgggcatca gttttttacc
    19921 gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac cagaagacaa
    19981 cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa tacgtagtga
    20041 ttctgacacg ttagttctta tccttaagcc cgggctttca gacgatctag tttttcaact
    20101 ttctgacgaa tacgttgttg tttcagcggt gacagataat ccataaacag tttgccgacc
    20161 aggtgatcca tctcatgctg aatacagatg gctaacagcc cctctgcttc cagttcaaat
    20221 ggtttaccgt cgcggtcaag ggcgcgaatt ttaactttct ctgcgcgcgg cactaaagca
    20281 cgttgttcag ggatcgacag gcaaccttct tcaatgcctg tttcgccgct tttttctaaa
    20341 agctccggat tgattaacac cagccgttcg tcacggtttt ccgaaacatc aataacaatg
    20401 atacgttgat ggatatcaac ctgggttgcc gccaggccaa taccttcttc tgcgtacatc
    20461 gtctcgaaca tatcatcgac gatacgctgt atttctgcat tcacttcttc taccggttta
    20521 gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga cataaatctc
    20581 cagagatgtg ttcaggggtt agaaagatta tttcttctat tctagacaaa tccccctctg
    20641 attgacagca tcactgacca atcgcaaaga ttgctaaggc tgcttatggc agggagataa
    20701 ggatggtcga tacagaaatt tggctgcgtt taatgagtat cagcagcttg tacggcgatg
    20761 atatggtccg tatagctcac tggctggcaa aacagtcgca aattgatgcg gttggattgc
    20821 agcaaacagg gcttacattg cggcaggcac aacgctttct ttcatttccg cgaaagagta
    20881 tcgaaagctc actttgttgg ttggagcaac ccaaccatca tttaatccct gcggacagcg
    20941 aattttatcc tcctcaactt ctggtgacga cagattaccc cggcgcactg tttgttgaag
    21001 gagaactgca cgcgctgcat tcatttcagc ttgccgtagt ggggagtcgg gcgcattcat
    21061 ggtatggcga gcgatgggga cggttatttt gcgaaactct ggcgacgcgt ggagtgacaa
    21121 ttacgagtgg actggcgcgt ggaatcgatg gtgtggcgca taaagctgcc ttacaggtaa
    21181 atggcgtcag cattgctgta ttggggaatg gacttaatac cattcatccc cgccgccatg
    21241 cccgactggc tgccagtctg cttgaacatg gcggagctct cgtctcggaa tttcccctcg
    21301 atgttccacc ccttgcttac aatttcccac gaagaaatcg cattatcagt ggtctaagta
    21361 aaggtgtact ggtggtggaa gcggctttgc gcagtggttc gctggtgaca gcacgttgtg
    21421 cgcttgagca gaggcgtgaa gtttttgcct tgccaggtcc aatagggaat ccgggaagcg
    21481 aagggcctca ctggttaata aaacaaggtg cgattcttgt gacggaaccg gaagaaattc
    21541 tggaaaactt gcaatttgga ttgcactggt tgccagacgc ccctgaaaat tcattttatt
    21601 caccagatca gcaagacgtg gcattgccat ttcctgagct cctggctaac gtaggagatg
    21661 aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca acctgtgcca gaggtagtta
    21721 ctcaactact cgaactggag ttagcaggat ggatcgcagc tgtacccggc ggctatgtcc
    21781 gattgaggag ggcatgccat gttcgacgta ctaatgtatt tgtttgaaac ctatattcac
    21841 actgaagctg agttgcgtgt ggatcaagac aaacttgaac aggatcttac cgacgcaggt
    21901 tttgagcgag aagatatcta caatgccctg ctatggctgg aaaaacttgc tgattatcag
    21961 gaagggttgg cagaaccgat gcaactggcc tcggatcctc tctccatgcg tatttataca
    22021 ccggaagagt gtgaacgact ggatgccagc tgccgtgggt ttctgctttt ccttgagcag
    22081 attcaggtgc tcaaccttga aactcgtgaa atggtgatag agcgagtgct cgcgctggat
    22141 aacgcagagt tcgaactgga tgatctgaaa tgggtgatcc tgatggtgtt gttcaacatc
    22201 ccggggtgcg aaaatgcgta ccagcaaatg gaagaattac tctttgaagt gaatgaaggt
    22261 atgctgcatt aatcatttct ttaattcagc ataagttgtt atggcaaaat cagcactgtt
    22321 cacggtgcgt aataatgagt cctgcccaaa gtgcggggct gaactggtta ttcgatccgg
    22381 gaaacacggt ccgtttcttg gatgctcaca gtatccggcg tgtgactacg tccgtcttct
    22441 gaaatcttca gcggatggac atattgtcaa agttctggag gggcaggttt gccctgcatg
    22501 tggcgcaaat ctggtattac gccagggacg ctttggtatg tttattggtt gcagtaacta
    22561 ccctgaatgc gaacataccg aacttatcga taaaccggac gaaacagcaa ttacatgccc
    22621 ccaatgtcgg acgggccatc tggtccagcg ccgctcccgt tatggcaaaa catttcactc
    22681 ctgtgatcgc tacccggagt gtcaatttgc cattaacttc aaaccaatag ctggagaatg
    22741 ccctgagtgt cattatccgc tactcatcga aaagaaaacc gcgcagggtg taaaacactt
    22801 ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa taataacgtg aataataacc
    22861 tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa tgaagaacgt gtcatcgcct
    22921 atccaacgga agccgttttc ggtgttgggt gcgatcctga tagcgaaaca gcagtgatga
    22981 gactgttgga gttaaaacag cgtccggttg ataaggggct gattttaatc gcggcaaatt
    23041 acgagcagct taaaccctat attgatgaca ccatgctgac tgatgcgcag cgtgaaacca
    23101 ttttttcccg ctggccaggt cctgtcacct ttgtctttcc cgcgcctgcg acaacaccgc
    23161 gctggttgac gggccgcttt gattcgcttg ctgtacgagt caccgaccat ccgttggtgg
    23221 ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac cagtgccaac ttgagtggat
    23281 tgccaccttg tcgaacagta gacgaagttc gcgcacaatt tggcgcggcg ttcccggttg
    23341 tgcctggtga aacggggggg cgtttaaatc cttcagaaat ccgcgatgcc ctgacgggtg
    23401 aactgtttcg acaggggtaa cataatggaa acctatgctg tttttggtaa tccgatagcc
    23461 cacagcaaat cgccattcat tcatcagcaa tttgctcagc aactgaatat tgaacatccc
    23521 tatgggcgcg tgttggcacc catcaatgat ttcatcaaca cactgaacgc tttctttcgt
    23581 gctggtggta aaggtgcgaa tgtgacggtg ccttttaaag aagaggcttt tgccagagcg
    23641 gatgagctta ctgaacgggc agcgttggct ggtgcagtta ataccctcaa gcggttagaa
    23701 gatggacgcc tgctgggtga caataccgat ggtgtaggct tgttaagcga tctggaacgt
    23761 ctgtctttta tccgccctgg tttacgtatt ctgcttatcg gcgctggtgg agcatctcgc
    23821 ggcgtactac tgccacttct ttccctagac tgtgcggtga caatcactaa tcggacggta
    23881 tcccgcgcgg aagagttggc taaattgttt gcgcacactg gcagtattca ggcgttgggt
    23941 atggacgaac tggaaggtca tgagtttgat ctcattatta atgcaacatc cagtggcatc
    24001 agtggtgata ttccggcgat cccgtcatcg ctcattcatc caggcattta ttgctatgac
    24061 atgttctatc agaaaggaaa aactcctttt ctggcatggt gtgagcagcg aggctcaaag
    24121 cgtaatgctg atggtttagg aatgctggtg gcacaggcgg ctcatgcctt ccttctttgg
    24181 catggcgttc tgcctgatgt agaaccggtc atcaaactat tgcaacagga attgtcagcg
    24241 tgaatcaggc catccagttt ccggataggg aagagtggga cgagaataaa agatgtgtat
    24301 gttttcctgc tctcgtgaat ggtatgcaac tgacatgtgc gatctctggc gagagtctgg
    24361 cgtatcgctt tactggaggt acgccagaac agtggttagc gagttttcgt cagcatcgct
    24421 gggacctgga agaagaagcg gaaaacttaa ttcaggaaca aagtgaagat gatcaaggtt
    24481 gggtctggtt accttgatcc agatattcgt ccttccattt cacgtagtta tttgcggaat
    24541 agcgtaaccc agccttctct tcatcactta acgggcggat ctgtttgacg ggactaccga
    24601 gatacagata tccgctctcc agccgtttat tttgtgggac caggctaccc gcaccaatca
    24661 tcacatcatc ttctactatt gcgccatcaa gtaaaattga gcccatccca accaaaactc
    24721 gattgccaat ggtgcagccg tggagcatca ccttgtgacc aacagtgaca tcttcgccaa
    24781 tggttaatgg gttgccatct gggttgtacg aggatttatg agtgacatgc aacatactgc
    24841 catcctggat attggtgcgt gctccgatct gtacataatg tacatctcca cgaatcacaa
    24901 cgagcggcca gatccccaca tcatcagcca gacgaacgtc accaatcacg acgctgctat
    24961 cgtcgatcat tacgcgctgg ccgatttgtg gaaaaagatc gcggtatggg cgtaaaacat
    25021 cagacatact cacctcaaca ataaatgatt tcctaatgac tttgggtgca ttattagcct
    25081 tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg tgcgaatgca gcaaaaaggg
    25141 tgaaaaaaca acaaacagaa aaaaagatca aaaaagtact tgtgcaaaaa attgggatcc
    25201 ctataatgcg cctccgttga gacgacaacg tgaaacactt cacaggatgg tcggaacaac
    25261 gaagagaaaa aattctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc
    25321 acctcgcgac agtgagctga aag
//
LOCUS       DAJTVE010000053        25035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000053 DAJTVE010000000
VERSION     DAJTVE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(82..492)
                     /locus_tag="OO439_002061"
     CDS             complement(82..492)
                     /locus_tag="OO439_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6316431.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(550..783)
                     /locus_tag="OO439_002062"
     CDS             complement(550..783)
                     /locus_tag="OO439_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6316432.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1172..1717
                     /locus_tag="OO439_002063"
     CDS             1172..1717
                     /locus_tag="OO439_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6316433.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1692..3617
                     /locus_tag="OO439_002064"
     CDS             1692..3617
                     /locus_tag="OO439_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6316434.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3614..3820
                     /locus_tag="OO439_002065"
     CDS             3614..3820
                     /locus_tag="OO439_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6316435.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3817..5418
                     /locus_tag="OO439_002066"
     CDS             3817..5418
                     /locus_tag="OO439_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6316436.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5399..6718
                     /locus_tag="OO439_002067"
     CDS             5399..6718
                     /locus_tag="OO439_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6316437.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6728..7060
                     /locus_tag="OO439_002068"
     CDS             6728..7060
                     /locus_tag="OO439_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6316438.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7116..8141
                     /locus_tag="OO439_002069"
     CDS             7116..8141
                     /locus_tag="OO439_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6316439.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8183..8581
                     /locus_tag="OO439_002070"
     CDS             8183..8581
                     /locus_tag="OO439_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6316440.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8593..8946
                     /locus_tag="OO439_002071"
     CDS             8593..8946
                     /locus_tag="OO439_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6316441.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8958..9536
                     /locus_tag="OO439_002072"
     CDS             8958..9536
                     /locus_tag="OO439_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316442.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9533..9928
                     /locus_tag="OO439_002073"
     CDS             9533..9928
                     /locus_tag="OO439_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316443.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9936..10676
                     /locus_tag="OO439_002074"
     CDS             9936..10676
                     /locus_tag="OO439_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316444.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10692..11114
                     /locus_tag="OO439_002075"
     CDS             10692..11114
                     /locus_tag="OO439_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6316445.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11096..11530
                     /locus_tag="OO439_002076"
     CDS             11096..11530
                     /locus_tag="OO439_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6316446.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11523..14084
                     /locus_tag="OO439_002077"
     CDS             11523..14084
                     /locus_tag="OO439_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6316447.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14081..14410
                     /locus_tag="OO439_002078"
     CDS             14081..14410
                     /locus_tag="OO439_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316448.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14410..15108
                     /locus_tag="OO439_002079"
     CDS             14410..15108
                     /locus_tag="OO439_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6316449.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15114..15857
                     /locus_tag="OO439_002080"
     CDS             15114..15857
                     /locus_tag="OO439_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316450.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15794..16426
                     /locus_tag="OO439_002081"
     CDS             15794..16426
                     /locus_tag="OO439_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6316451.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16487..19984
                     /locus_tag="OO439_002082"
     CDS             16487..19984
                     /locus_tag="OO439_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6316452.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20055..20571
                     /locus_tag="OO439_002083"
                     /pseudo
     CDS             20055..20571
                     /locus_tag="OO439_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20636..22040
                     /locus_tag="OO439_002084"
                     /pseudo
     CDS             20636..22040
                     /locus_tag="OO439_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            21989..22141
                     /locus_tag="OO439_002085"
                     /pseudo
     CDS             21989..22141
                     /locus_tag="OO439_002085"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22118..22297
                     /locus_tag="OO439_002086"
                     /pseudo
     CDS             22118..22297
                     /locus_tag="OO439_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23012..23719
                     /locus_tag="OO439_002087"
                     /pseudo
     CDS             23012..23719
                     /locus_tag="OO439_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23719..24300
                     /locus_tag="OO439_002088"
     CDS             23719..24300
                     /locus_tag="OO439_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6316453.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         6014 a         6172 c         7529 g         5320 t
ORIGIN      
        1 gcacaccggg gttaccgggt acacatattt catcatgcca gcgagtccgg ttctggcacg
       61 gaagaaaccg gacgttatga tttagtgcgg aaatatttgt gtagtgttct gtatgttctc
      121 agtaaagagt aatgaattat caaaggtata gtaatacctt ttgttttcgt ggatatttgt
      181 aatccatctg aaaacccctg ctgtagcaag atttttcctg tattcgtaaa atgataactc
      241 tcctgatttg aatcctttta aggtggcttc tataaggcat ttattttttg aaaatcttac
      301 atttacaacc ttaccctgtc cttttattaa aaccgtatta tcgttttcaa gaacaagatg
      361 aatattctct gtggctaaat agtaaatgta atgtgagaca ttgtgacgtt ttagttcaga
      421 ataaaaccag tgatagttta aattatttcg cactttatcg aatatttgtt taaaaatggc
      481 aacctgagcc attgtagtac cttccatgtg atatgagggg gcgtagtctg cacgattatc
      541 taaattgctt caatctggtc taacctgttt tctgagcaat tcagtaatgt cactcttttc
      601 tttgtttgct tcaggcgaaa ctcttttttc tgagcacagt ctccggcggc aggcttcaat
      661 gacccaggct gagaaattcc cggacccttt ttgaacaaga gcgatgttaa tttgttcaat
      721 catttggtta ggaaagcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga
      781 catgaggttg ccctgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt
      841 aagttgatgc agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt
      901 gatggcgtag atgcacgttg tgacatgtag atgataatta ttatcatttt gcgggtcctt
      961 tccggcgatc cgacaggtta cggggcggaa ggcgcgcggg ttttcgctat ttatgacaat
     1021 tttccggttt aaggcgtttc cgttcttctt cgccgtaacc taatgttttt atttaaaaca
     1081 ccccctgaaa agaaaggaaa cgacaggtgc tgaaaacggg ctttttggcc tctgtcgttt
     1141 cctttctctg tttttgtccg tggaatgaac aatggaagtc aacaaaaagc agctggctga
     1201 cattttcggt gcgagtatcc gtaccattca gaactggcag gaacagggaa tgcccgttct
     1261 gcgaggcggt ggcaagggta atgaggtgct ttatgactct gccgccgtta taagatggta
     1321 tgccgaaagg gatgctgaaa ttgagaacga aaagctgcgc cgggaagttg aagaactgcg
     1381 gcaggccagc gagacagatc tccagccagg gactattgag tacgaacgcc atcgacttac
     1441 gcgtgcgcag gccgacgcac aggagctgaa aaatgccaga gactccgctg aagtggtgga
     1501 aaccgcattc tgtactttcg tgctgtcgcg gatcgcaggt gaaattgcca gtattctcga
     1561 cgggatcccc ctgtcggtgc agcggcgttt tccggaactg gaaaaccgac atgttgattt
     1621 cctgaaacgg gatatcatca aagccatgaa caaagcagcc gcgctggatg aactgatacc
     1681 ggggttgctg agtgaatata tcgaacagtc aggttaacag gctgcggcat tttgtccgcg
     1741 ccgggcttcg ctcactgttc aggccggagc cacagaccgc cgttgaatgg gcggatgcta
     1801 attactatct cccgaaagaa tccgcatacc aggaagggcg ctgggaaaca ctgccctttc
     1861 agcgggccat catgaatgcg atgggcagcg actacatccg tgaggtgaat gtggtgaagt
     1921 ctgcccgtgt cggttattcc aaaatgctgc tgggtgttta tgcctacttt atagagcata
     1981 agcagcgcaa cacacttatc tggttgccga cggatggtga tgccgagaac tttatgaaaa
     2041 cccacgttga gccgaccatc cgcgatattc cgtcgctgct ggcgctggct ccgtggtatg
     2101 gcaaaaagca ccgggataac acgctcacta tgaagcgttt ttccaatggt cgtggcttct
     2161 ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat gtggcgggtt
     2221 atgatgaact tgctgccttt gatgaggata ttgaacagga aggctctccg acgttccttg
     2281 gcgacaaacg tattgaaggc tcggtctggc caaagtccat ccgtggctcc acgcccaaag
     2341 tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc cccgcatttt atgcgttttc
     2401 atgttgcctg cccgcactgc ggggaggagc agtatcttaa atttggcgac aaagagacgc
     2461 cgtttggcct caaatggacg ccggatgacc cctccagcgt gttttatctc tgcgagcata
     2521 atgcctgcgt catccgccag caggagctgg actttactga tgcccgttat atctgcgaaa
     2581 agaccgggat ctggacccgt gatggcattc tctggttttc gtcatccggt gaagagattg
     2641 agccacctga cagtgtgacc tttcacatct ggacagcgta cagcccgttc accacctggg
     2701 tgcagattgt caaagactgg atgaaaacga aaggggatac gggaaaacgt aaaaccttcg
     2761 taaacaccac gctcggtgag acgtgggagg cgaaaattgg cgaacgtccg gatgctgaag
     2821 tgatggcaga gcggaaagag cattattcag cgcccgttcc tgaccgtgtg gcttacctga
     2881 ccgccggtat cgactcccag cgggatcgct acgaaatgcg cgtatgggga tgggggccgg
     2941 gtgaggaaag ctggctgatt gatcggcaga ttattatggg ccgccacgac gatgaacaga
     3001 cgctgctgcg tgtggatgag gccatcaata aaacctatac ccgccggaat ggtgcagaaa
     3061 tgtcgatatc ccgtatctgc tgggatattg gcgggattga cccgaccatt gtgtatgaac
     3121 gctcgaaaaa gcatgggctg ttccgggtga tccccattaa aggggcatcc gtctacggta
     3181 agccggtggc cagcatgcct cgtaagcgaa acaaaaacgg ggtttacctt accgaaatcg
     3241 gtacggatac cgcgaaagag cagatttata accgcttcac actgacgccg gaaggggatg
     3301 aaccgcttcc cggtgccgtt cacttcccga ataacccgga tatttttgat ctgaccgaag
     3361 cgcagcagct gactgctgaa gagcaggtcg aaaaatgggt ggatggcagg aaaaaaatac
     3421 tgtgggacag caaaaagcga cgcaatgagg cgctcgactg cttcgtttat gcgctggcgg
     3481 cgctgcgcat cagtatttcc cgctggcagc tggatctcag tgcactgctg gcgagcctgc
     3541 aggaagagga tggtgcagca accaacaaga aaacactggc agattacgcc cgtgccttat
     3601 ccggagagga tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg cactgcatga
     3661 cctgatgaca ggtaaacggg tggcaacggt acagaaagac ggacggcgag tggagtttac
     3721 ggccacttcc gtgtctgacc tgaaaaaata cattgcggag ctggaggtgc agaccggcat
     3781 gacacagcga cgcaggggac ctgcaggatt ttatgtatga aaacgcccac cattcccacc
     3841 cttctgggac cggacggcat gacatcgctg cgtgaatatg ccggttatca cggcggtggc
     3901 agcggatttg gtgggcagtt gcgggcgtgg aacccaccgg gtgaaagtgt ggatgcagcc
     3961 ctgctgccca actttacccg tggcaatgcc cgcgcagacg atctggtacg caataacggc
     4021 tatgctgcca acgccatcca gctgcatcag gatcatatcg tcgggtcttt tttccggctc
     4081 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagacgcccg tgccttttcc
     4141 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     4201 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     4261 ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag
     4321 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
     4381 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
     4441 gacggctatc ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc
     4501 gggcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac ccgcggtgca
     4561 aatgtgtttt acagcgtaat ggagcagatg aagatgctcg acacgctgca gaacacgcag
     4621 ctgcagagcg ccattgtgaa ggcgatgtat gccgccacca ttgaaagtga gctggatacg
     4681 cagtcagcga tggattttat tctgggcgcg aacagtaagg agcagcggga aaggctgacg
     4741 ggctggattg gtgaaattgc cgcgtattac gccgcagcac cggtccgtct gggaggcgca
     4801 aaagtgccgc acctgatgcc gggggactca ctgaacctgc agacggctca ggacacggat
     4861 aacggctact ccgtgtttga gcagtcactg ttgcggtata tcgctgccgg gctgggtgtc
     4921 tcgtatgagc aactttcccg gaattacgcc cagatgagct actccacggc acgggccagt
     4981 gcgaacgagt cgtgggcgta ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg
     5041 agccagatgt ttctgtgctg gctggaagag gccatcgttc gccgcgtggt gacgttacct
     5101 tcaaaagcgc gcttcagctt tcaggaagcc cgcagtgcct gggggaactg cgactggata
     5161 ggctccggtc gtatggccat cgatggtctg aaagaagttc aggaagcggt gatgctgata
     5221 gaagccggac tgagcaccta cgagaaagag tgcgcgaaac gcggtgacga ctatcaggaa
     5281 atttttgccc agcaggtccg tgaaacgatg gagcgccgcg cagctggtct taaaccgccc
     5341 gcctgggcgg ctgcggcatt tgaatccggg ctgcgacaat caacagagga ggagaagagt
     5401 gacagcagag ctgcgtaatc tcccgcatat tgccagcatg gcttttaatg agccgctgat
     5461 gcttgaaccc gcctatgcgc gggttttctt ttgtgcgctt gcaggccagc ttgggatcag
     5521 ctgcctgacg gatgcagtat ccggcgacag cctgactgcc ggagaggcac ccgcggcgct
     5581 ggcgttatcc ggtgatgatg acggaccacg acaggcccgc agttatcagg taatgaacgg
     5641 catcgccgtg ctgccggtgt ccggcacgct ggtcagccgg acgcgggcgc tgcagccgta
     5701 ctcggggatg accggttaca acggcattat cgcccgtctg caacaggctg ccagcgaccc
     5761 gatggtggac ggcattctgc tcgatatgga cacaccgggc ggaatggtgg cgggagcatt
     5821 tgactgcgct gacatcatcg cccgtgtgcg tgacataaag ccggtatggg cgctggccaa
     5881 tgacatgaac tgcagcgcag gtcagctgct tgccagtgcc gcctcccggc gtctggtcac
     5941 gcagaccgcc cggacaggct ccatcggcgt catgatggct cacagtaatt acggcgctgc
     6001 actggagaaa cagggcgtgg aaatcacgct gatttacagc ggcagccata aggtggatgg
     6061 caacccctac agccatctac cggatgatgt ccgggaaaca ttgcagtccc ggatggatgc
     6121 aacccgccgg atgtttgcac agaaggtgtc ggcatatacc ggcctgtccg tgcaggctgt
     6181 gctggatacc gaggctgcag tgtacagcgg tcaggaggcc attgatgccg gactggctga
     6241 tgaacttgtt aacagtaccg atgcgatcac cgtcatgcgt gatgcactgg atgcacgtaa
     6301 atcccgtctc tcaggagggc gaatgaccaa agagactcaa tcaacaactg tttcagccac
     6361 tgcttcgcag gctgacgtta ctggcgtggt gcaagcgacg gagggcgaga acgccagcgc
     6421 ggcgcagccg gacgtgaacg cgcagatcac cgcagcggtt gcggcagaaa acagtcgcat
     6481 tatggggatc ctcaactgtg aggaggctca cggacgcgaa gaacaggcac gcgtgctggc
     6541 cgaaaccccc ggtatgaccg tggaaacggc ccgccgtatt ctggccgcag caccacagag
     6601 tgcacagacg cgcagtgaca ctgcgctgga tcgtctgatg cagggggcac cggcaccgct
     6661 ggctgcaggt aacccggcat ctgatgccgt taacgatttg ctgaacacac cagtgtaagg
     6721 gatgtttatg acgagcaaag aaacctttac ccattaccag ccgctgggca atagtgaccc
     6781 ggctcatacc gcaaccgcgc ccggcggatt gagtgcgaaa gcgcctgcaa tgaccccgct
     6841 gatgctggac acctccaccc gtaagctggt tgcgtgggat ggcaccaccg acggtgctgc
     6901 cgttggcatt cttgcggttg ctgctgacca gaccagcacc acactgacgt tctacaagtc
     6961 cggcacgttc cgttatgagg atgtgctctg gccggaggct gccagcgacg agacgaaaaa
     7021 acggaccgtg tttgccggaa cggcaatcag catcgtttaa ctttaccctt catcactaaa
     7081 ggccgcctgt gtggcttttt ttacgggatt tttttatgtc gatgtacaca accgcccagc
     7141 tgctggcggc aaatgagcag aaatttaagt ttgatccgct gtttctgcgt ctctttttcc
     7201 gtgagagcta tcccttcact acggagaaag tctatctctc acaaattccg ggactggtaa
     7261 acatggcgct gtacgtttcg ccgattgttt ccggtgaggt tatccgttcc cgtggcggct
     7321 ccacctctga atttacgccg ggatatgtca aacccaagca tgaggtgaat ccgcagatga
     7381 ccctgcgtcg cctgccggat gaagatccgc agaatctggc ggacccggct taccgccgcc
     7441 gtcgcatcat catgcagaac atgcgtgacg aagagctggc cattgctcag gtcgaagaga
     7501 tgcaggcagt ttctgccgtg ctcaagggca aatacaccat gaccggtgaa gccttcgatc
     7561 cggttgaggt ggatatgggc cgcagtgcgg cgaacaacat cacacagtcc ggcgtcacgg
     7621 agtggagcaa gcgtgacaag tccacgtatg acccgaccga cgatatcgaa gcctacgcgc
     7681 tgaacgccag cggtgtggtg aatatcatcg tgtttgaccc gaaaggctgg gcgctgttcc
     7741 gttccttcaa agccgtcaag gagaagctgg atacccgtcg cggctctcat tccgagctgg
     7801 agacagcggt aaaagacctg ggcaaagcgg tgtcctataa gggaatgtat ggcgatgtgg
     7861 ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt caaaaagaac ttcctgccgg
     7921 acaacacgat ggtgctgggg aacactcagg ctcgcggtct gcgcacctat ggctgcattc
     7981 aggatgcgga cgcacagcgc gaaggcatta acgcttctgc ccgttacccg aaaaactggg
     8041 tgaccaccgg cgatccggcg cgtgagttca ccatgattca gtcagcaccg ctgatgctgc
     8101 tggctgatcc tgatgagttc gtgtccgttc aactggcgta atcatggccc ttcggggcca
     8161 ttttctctct gtggaggagt tcatgacgaa agatgaactg attgcccgtc ttcaggagct
     8221 gggtgagcaa ttgaaccgcg atgtcagttt gacgggaacg aaagaagaac tggcgcttcg
     8281 tgtggcagag ctggaagaag agcttgatga cacggatgaa actgccggtc aggacacccc
     8341 tctcagccgg gaaaatgtgc tgaccgggca tgaaaatgag gtggtatcag cgcagccgga
     8401 taccgtgatt caggatacgg ctgaactggt cacggtcgtg gcactggtga cgctgcatac
     8461 tgatgcactt cacgccacgc gggataaacc tgtggcattt gtgctgccgg gaacggcgtt
     8521 tcgtgtctct gccggtgtgg cagccgaaat gacagagcgc ggcctggcca gaatgcaata
     8581 acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc ccgcgccgat
     8641 gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga gcagtcaggt
     8701 gcggtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg acagggcgtg
     8761 cgcgttgaag gctccagccc gtccctgttt gtccggactg atgaggtgcg gcagctgcgg
     8821 cgtggagaca cgctgaccat cggtgaggaa aatttctggg tagatcgggt ttcgccggat
     8881 gatggcggaa gttgtcatct ctggcttgga cggggcgtac cgcctgccgt taaccgtcgc
     8941 cgctgaaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa aacctcagcc
     9001 gtatcagcaa aacggcggtg cccggtgccg ccgcaatggc cattaaccgc gttgcttcat
     9061 ccgcgatatc gcagtctgcg tcacaggttg cccgtgagac aaaggtacgc cggaaactgg
     9121 taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc agaatcaaag
     9181 ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc ctgtcccgcc
     9241 gcaggcgtca taaaaagggg cagcgttcat ccctgaaagg tggcggcagc gtgcttgtgg
     9301 tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaacggc cggtggcatg
     9361 ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa atcccgatgg
     9421 cggtgccgct gaccacggcg tttaaacaga atattgaacg gatacggcgt gaacgtcttc
     9481 cgaaagagct gggctatgcg ctgcagcatc aactgagaat ggtaataaag cgatgaaaca
     9541 tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg gggcgacgct
     9601 ttttgatggt cgccccgctg tttttgatga ggaagatttt ccggcaattg ccgtttatct
     9661 caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg cggagctgca
     9721 tattgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggattcgt ggatggagtc
     9781 ccggatttat cctgtgatga gcgatatccc ggcactgtca gatttgatca ccagtatggt
     9841 ggccagcggc tatgactacc ggcgcgacga tgatgcgggc ctgtggagtt cagccgatct
     9901 gacttatgtc attatctatg aaatgtgagg acgctatgcc tgtaccaaat cctacaatgc
     9961 cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac ccttatgcga
    10021 acccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg acgcccggcg
    10081 aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgca gactggactg
    10141 cgaccgggca ggggcagaaa tctgccggag ataccagctt cacgctggcg tggatgcccg
    10201 gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc cgtgcctata
    10261 aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc agtatcggta
    10321 aggcggtgac ggcgaaggag gtgattaccc gcacggtgaa ggtcaccaat gtgggacgtc
    10381 cgtcgatggc agaagatcgc agcacggtga cggcgacgac cggcatgacg gtgacacccg
    10441 ccagtgcttc cgtagtgaaa gggcagagca ccacgctgac cgtggcattc cagccggatg
    10501 gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc accgtgtcgg
    10561 tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac attccggtcg
    10621 tatctggtaa tggtgagttt gctgcggttg cagaaatcaa cgtcaccgcc agttaatccg
    10681 gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggtgtg accgtcacgc
    10741 tttctgaact gtcagccctg cagcgtattg agcatctcga cctgatgaaa cggcaggcag
    10801 aacaggcgga gtcagacagc aaccggaagt ttactgtgga agacgccatc agaaccggcg
    10861 cttttgtggt tgcgatgtcc ctgtggcata accatccgaa gaagacgcag atgccgtcca
    10921 tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc acagaggcaa
    10981 tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt gtggtgaatg
    11041 atgctcctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg ggaaagtgtt
    11101 cgacggtgag ctgagttttg ccctgaaact ggcgcgtgag atggggcgac ccgactggcg
    11161 cgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct tttacagtac
    11221 ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt acaccgtgct
    11281 cagcctgttt ttcagcgatc cggagatgca tccgctggat ttcagtctgc tgaaccggcg
    11341 cgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag ggcttgccgg
    11401 aggtgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg atgtggcgga
    11461 catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag gaggagtccg
    11521 gtatggctga accggtaggc gatctggtcg ttgatttgag tctggatgcg gccagatttg
    11581 acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat gcgaaaaaaa
    11641 cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag aaagcgggga
    11701 tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc accgacgtgg
    11761 ccacgcagct tgcagggggg cagaatccct ggctgatcct gctgcaacag ggtggtcagg
    11821 ttaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggc gcgatcaccc
    11881 tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg tatgcctggt
    11941 atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc ggtaatcagg
    12001 cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg gcagggctga
    12061 cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta agcggtgagg
    12121 ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctatgcatcc ggcgtggagg
    12181 tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg gggctgacgg
    12241 cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt gctcagttgc
    12301 agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca acgaaagggt
    12361 ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc tgggcagaca
    12421 ggactgcacg tgcattcaaa tccatgtggg atgcggtgct ggatattggt cgtcctgata
    12481 ccgcgcagga gatgctgatt aaggcagagg ctgcgtttaa gaaagcagac gacatctgga
    12541 atctgcgcaa ggatgattat ttcgttaacg atgaagcacg ggcgcgttac tgggatgatc
    12601 gtgaaaaaaa acgcctggag cgggatgcag cccaaaagag ggtagatcaa cagcgtcaac
    12661 aggacaaaaa tgcgcagcag cagagcgata ccgaagcgtc acggctgaaa tataccgaag
    12721 aggcgcagaa ggcttacgaa cggctgcaga cgccgctgga gaaatatacc gcccgtcagg
    12781 aagaactgaa caaggcactg aaagacggaa aaatcctgca ggcagattac aacacgctga
    12841 tggcggcggc gaaaaaggat tatgaagcga cgctgaaaaa gccgaaacag tccggcgtga
    12901 aggtgtatgc gggcgatcgt caggaagaca gtgctcatgc ggccctgctg acgcttcagg
    12961 cagaactccg gacgctggag aagcatgccg gagcgaatga gaaaatcagc cagcagcgcc
    13021 gggatttgtg gaaggcagaa agtcagttcg cggtactgga ggaggcggca caacgtcgcc
    13081 agctgtccgc acaggagaaa tccctgctgg cgcataaaga cgagacgctg gagtacaaac
    13141 gccagctggc tgcacttggt gacaaggtta cgtatcagga gcacctgaat gcgctggcgc
    13201 agcaggcgga taaattcgca cagcagcaac gggcaaaacg ggcagccatt gatgcgaaaa
    13261 accgggggct gactgaccgg caggcagcgc gggaagccac ggaacagcgc ctgaaggaac
    13321 agtatggcga taatcctctg gcgctgaata acgtcatgtc agagcagaaa aagacctggg
    13381 cggctgaaga ccagcttcgc gggagctgga tggcaggcct gaagtccggc tggagtgagt
    13441 gggaagagag cgccacggac agtatgtcac aggttaaaag tgcagccacg cagacctttg
    13501 atggtattgc acagaatatg gcggcgatgc tgaccggcag tgaacagaac tggcgcagct
    13561 tcacccgctc cgtgctgtcc atgatgacag aaattctgct taagcaggca atggtgggga
    13621 ttgtcgggag tatcggcagc gccattggcg gggctgttgg tggcggcgca tccgcgtcag
    13681 gtggtacagc cattcaggcc gctgcggtga aattccattt tgcaaccggg ggatttacgg
    13741 gaaccggcgg caaatatgag ccagcgggga ttgttcaccg tggtgaattt gtcttcacga
    13801 aggaggcaac cagccggatt ggcgtgggga atctctaccg gctgatgcgc ggctatgcgg
    13861 aaggtggtta tgtgggcggt gccggaagtc cggcgcagat gcggcgggct gaaggcatta
    13921 attttaatca gaacaatcac gtggtgattc agaacgacgg tacgaatggt ctgccaggtc
    13981 cacagatgat gaaggccgtg tatgacatgg cccgcaaggg tgcccgtgat gaaattcaga
    14041 cacagatgcg tgatggtggc ctgttctccg gaggtggacg atgaaaacct tccgctggaa
    14101 agtgaaaccc ggtatggatg tggcttcggc cccttctgta agaaaggtgc gctttggtga
    14161 tggctattcc cagcgagcgc ctgccgggct gaatgccaac ctgaaaacgt acagcgtgac
    14221 gctttctgtc ccccgtgagg aggccatggt actggagtcg tttctggaag agcacggggg
    14281 ctggaaagcc tttctgtgga cgccgcctta tgagtggcgg cagataaagg tgacctgcgc
    14341 aaaatggtcg tcgcgggtca gtatgctgcg tgttgagttc agcgcagagt ttgaacaggt
    14401 ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg tgcggagcag
    14461 tcggccagcg tggtgctctg ggaaatcgat ctgacagagg tcggtggaga acgttatttt
    14521 ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg acagtatcag
    14581 ccgtatccca ttcaggggag tggttttgaa ctgaatggca aaggcaccag tacgcgcccc
    14641 acgctgatgg tttctaacct gtacggtatg gtcaccggga tggcggaaga tctacagagt
    14701 ctggtcggcg gaacggtggt ccggcgtaag gtttacgccc gttttctgga tgcggtgaac
    14761 ttcgtcaacg gaaacagtga cgccgatccg gagcaggagg tgatcagccg ctggcgcatt
    14821 gagcagtgca gcgaactgag cgcggtgagt gcctcttttg tactgtccac gccgacggaa
    14881 acagatggcg ctgtttttcc gggacgtatc atgctggcca acacctgcac ctggacctat
    14941 cgcggcgatg agtgcggtta tcacggtccg gcggtcgcgg atgaatatga ccagccaacg
    15001 tccgacatca cgaaggataa atgcagcaaa tgcctgagtg gctgtaagtt tcgcaataac
    15061 gtcggcaact ttggcggctt cctttccatt aacaaacttt cgcagtaaat cccatgacag
    15121 agacagaatc agcgattctg gcgcacgccc ggcgatgtgc tccagcggag tcgtgcggct
    15181 tcgtggtaag aacgccggag ggggaaagat atttcccctg cgtgaatatc tccggtgagc
    15241 cggaggcgta tttccgtatg tcgccggaag actggctgca ggcagagatg cagggtgaga
    15301 ttgtggcact ggtccacagc caccccggtg gtctgccctg gctgagtgag gccgaccggc
    15361 ggctgcaggt gcagagtgat ttgccgtggt ggctggtctg ccggggggcg attcacaagt
    15421 tccgctgtgt gccacatctt accgggcggc gctttgagca cggggtgacg gactgttaca
    15481 cgctgttccg ggatgcttac catctggcgg ggattgagat gccggatttt catcgtgagg
    15541 atgactggtg gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt
    15601 atcaggtgcc gttgtcagcg gcgcagccgg gcgatgtgct gctgtgctgt tttggttcat
    15661 cggtgccgaa tcatgccgcc atttactgtg gtgacggcga gctgctgcac catattcctg
    15721 aacaactgag caaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct
    15781 ggcgtcaccg ggcatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg
    15841 catcgacctt cgtgtgaaaa cgggtgccga agccatccgg gcgatggcca cacagctccc
    15901 tgcgtttcgt cagaagctga gcgacggctg gtatcaggtg cgtattgccg ggcaggatgt
    15961 tagcacgtcc ggattaacgg cacagttaca tgaggctctg cctgacggcg ctgtgattca
    16021 tattgttccc agagtcgccg gggccaagtc aggtggtgta ttccagattg tcctgggagc
    16081 agccgccatt gccggatcat tctttaccgc cggagccacc cttgcagcat ggggggctgc
    16141 cattggtgcc ggtggtatga ccggcattct gttttctctc ggtgccagta tggtgctcgg
    16201 cggtgtggct cagatgctgg caccgaaagc cagaactccc cgcacacaga caacggataa
    16261 cggtaagcag aacacctatt tctcctcact ggataacatg gttgcccagg gcaatgttat
    16321 gcctgttctg tatggtgaaa tgcgcgtggg gtcacgtgtg gtatctcagg agatcagcac
    16381 ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt cgctgatgca aaatgtttta
    16441 tgtgaaatcg cctccgggcg gttttatcgt ttatggagca tgacgaatgg gtaaaggcag
    16501 cagtaagggg cataccccgc gcgaagcgaa ggacaacctg aagtccacgc agctgctgag
    16561 tgtgatcgat gccatcagcg aagggccggt tgaaggtccg gtggatggat taaaaagcgt
    16621 gctgctgaac agtacgccgg tgctggacag tgaggggaat accaacatct ccggcgtcac
    16681 ggtggtgttc cgggcaggtg agcaggagca gtcaccgccg gagggatttg aatcctccgg
    16741 ctccgagacg gtgctgggta cggaagtgaa atatgacacg ccgatcaccc ggaccatcac
    16801 gtcggcaaac attgaccgac tgcgttttac cttcggtgta caggcactgg tggaaaccac
    16861 ctcaaaggga gacaggaatc cgtctgaagt tcgcctgctg gttcagatac agcgtaacgg
    16921 tggctgggtg acggaaaaag acatcaccat taagggcaaa accacctcgc agtatctggc
    16981 ctcggtggtg gtggataacc tgccgccgcg cccgtttaat atccggatgc gcagaatgac
    17041 gccggacagc accacagacc agctgcagaa caaaacgctc tggtcgtcat acaccgaaat
    17101 catcgatgtg aaacagtgct acccgaacac ggcactggtc ggcgtacagg tggattcgga
    17161 gcagttcggc agccagcagg tgagccgtaa ttatcatctt cgcgggcgca ttctgcaggt
    17221 gccgtcgaac tataatccgc agacgcggca atacagcggt atctgggacg gaacgttaaa
    17281 accggcatac agcaacaaca tggcctggtg tctgtgggat atgctgaccc atccgcgcta
    17341 tggcatgggg aaacgtcttg gtgcggcaga tgtggataaa tgggcgctgt atgtcatcgg
    17401 ccagtactgt gaccagtcgg tgccggacgg ctttggcagc acggagccgc gcatcacctg
    17461 taatgcctgg ctgaccacac agcgcaaggc gtgggatgtg ctcagtgatt tctgctcggc
    17521 gatgcgctgt atgccggtat ggaacgggca gacgctgacg ttcgtgcagg accgacagtc
    17581 ggataaggtg tgggcctata accgcagtaa tgtggtgatg ccggatgatg gcgcgccgtt
    17641 ccgctacagt ttcagcgccc tgaaagaccg ccataatgcc gttgaggtga actggattga
    17701 cccggataac ggctgggaga cggcgacaga gcttgtggag gatacgcagg ccattgtccg
    17761 ttacggtcgt aacgtcacga agatggatgc ctttggctgt accagccggg ggcaggcaca
    17821 ccgcgccggg ctgtggctga ttaaaacgga actgctggag acgcagaccg tggatttcag
    17881 cgtgggtgct gaagggcttc gtcatgtacc gggcgatgtc attgaaatct gcgatgatga
    17941 ctatgccggt atccgcaccg gcgggcgcgt gctggcggtg aacagccaga cccggacgct
    18001 gacgctcgac cgtgaaatca cgctgccatc ctccggcacc acgctgataa gcctggttga
    18061 cgggcagggg aatccggtca gcgtggaggt ccagtccgtc accgacggcg tgaaggtaaa
    18121 agtgagccgt gttcctgacg gtgttgctga atacagcgtg tgggggctga agctgccgac
    18181 gctgcaccag cgattgttcc gctgcgtgag tatccgtgag aacgacgacg gcacgtatgc
    18241 catcaccgcc gtgcagcatg tgccggaaaa agaagccatc gtggataacg gggcgcactt
    18301 tgacggcgac cagagcggca cggtgaatgg tgtcacgccg ccagcggtgc agcacctgac
    18361 tgccgaagtc accgcagaca gcggggaata tcaggtgctg gcgcgctggg acacgccgaa
    18421 ggtggtgaag ggcgtgagct tcctgctccg tctgaccgta acagcggatg acggcagtga
    18481 gcggctggtc agcacggccc ggacgacgga aaccacttac cgcttcacac aactggcgct
    18541 ggggaactac aggctgacag tccgggcagt aaatgcgtgg gggcagcagg gcgatccggc
    18601 gtcggtatcg ttccggattg ccgcaccggc agcgctgtcg cggattgagc tgacgccggg
    18661 ctattttcag ataaccgcca cgccgcatct tgcggtttat gatccgacgg tacagtttga
    18721 gttctggttc tcggaaaagc ggattgcgga tatcaggcag gttgaaacca cagcacgcta
    18781 tcttggcacg gcgctgtact ggatagccgc cagtatcaat atcaaaccgg gccatgatta
    18841 ttacttttat atccgcagtg tgaacaccgt tggcaaatcg gcattcgtgg aggctgttgg
    18901 tcagccgagt gatgatgcat ccggttatct ggattttttc aaaggcgaga tagggaaaac
    18961 ccatctggct caggagctgt ggacgcagat tgataacggt cagcttgcgc ctgacctggc
    19021 tgaaatcagg acgtccatta cggatgtcag caatgaaatc acgcagaccg tcaataagaa
    19081 actggaagac cagagtgcgg caattcagca gatacagaag gttcaggttg atacaaataa
    19141 taacctgaac agcatgtggg ctgtgaagct gcagcagatg caggacggac gcctttatat
    19201 cgcgggtatt ggtgccggta ttgagaacac ccctgacggc atgcagagtc aggtgctgct
    19261 ggcggcggac aggattgcga tggttaatcc tgcgaatggc aacacaaaac cgatgtttgt
    19321 tggtcagggc gatcagatat tcatgaacga cgtgttcctg aaacgcctga cggcccccac
    19381 cattaccagc ggtggaaatc catcggcatt ttccctgact ccggacggaa agctgaccgc
    19441 taaaaatgcg gatatcagtg gcagtgtgaa tgcgaacgcc gggacgctca acaatgtcac
    19501 ggtaaatgaa aactgtacga ttaagggcat gctggaggcg actcaggtca gaggtgactt
    19561 cgttaaagct gtatccaaat catttccgaa acaggctggt acgtggggta acacggaaac
    19621 accaaacggg acggttacag tcaccataag cgatgatcat aactttgacc gccagattat
    19681 tattccgccc attattttta acggtatagc gtatgacgat ccggggagcg gaaataaccc
    19741 aggaggcacg cgatacacgg gttatggttt tgaagttcgc aaaaacggcg tattaatcgc
    19801 atccagagaa actaaaggtg ccattcccgg tagttacagt gcagttattg atatgcctag
    19861 tggtggtggt agcgtcactc tggagtttaa gattttccag aaaggcaatc agggggcagg
    19921 caatatcacc gactgtacgg tgattgtgac caaaaaagcg gcttccggca tcagtattcg
    19981 ttgaaatatt tataacccca ataaatggcg tcaggaatga cgcctttttt attgcagaaa
    20041 agcgagaggt aattatgcgt aaagtttgtg cagccatttt gtccgcagcc atttgcctgg
    20101 ccgtatccgg tgcgcctgca tgggcgtctg aacatcagtc cacgctgagc gcggggtatc
    20161 ttcatgcccg gaccaacgtt cccggcagtg atgatctgaa cgggattaac gtgaaatacc
    20221 gttatgagtt tacggatacg ctggggctgg tgacgtcatt cagctatgca ggagacaaga
    20281 atcgccagct gacccgttac agcgataccc gctggcatga agattccgtg cgtaaccgct
    20341 ggttcagcgt gatggcgggg ccgtctgtgc gcgtgaatga atggttcagc gcgtatgcga
    20401 tggcgggtgt ggcttacagc cgtgtgtcga ctttttccgg ggattatatc cgcgtaactg
    20461 acaataaggg gaaaacgcac gatgtgctga ccggaagtga tgacggtcgc cacagcaaca
    20521 cgtctctggc gcactgacgg tttcatcgtg ggtgtcggtt ataaattctg attagccagg
    20581 taacacagtg ttatgacagc ccgccggttc aggcgggctt ttttgtgggg tgaatatggc
    20641 agtaaagatt tcaggtgtac tgaaagacgg cacaggaaaa ccggtacaga actgcacaat
    20701 ccagctgaaa gcaaaacgta acagcaccac ggtggtggtg aacacgctgg cctcagaaaa
    20761 tccggatgaa gccgggcgtt acagcatgga cgttgagtac ggtcagtaca gcgttattct
    20821 gttggtggaa ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattcccg
    20881 acccggtacg ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc
    20941 actgcgccgt tttgagctga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca
    21001 gaacacggca gccgcgaaga aatcagccag cgatgccagc acatcagccc gtgaggcggc
    21061 aacccatgcg actgatgctg cagattcagc acgtgcagcc agcacgtcag ccggacaggc
    21121 cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa aggccactga
    21181 agcctcaaaa agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg
    21241 tgcggcgaaa acgttagaaa cgaatgcggc agcgtcacaa caatcagcag ccacttctgc
    21301 atccaccgcg accacgaaag cgtcagaagc tgccacctca gcccgggatg cgtcggcttc
    21361 aaaagaggcg gcaaaatcat cagaaacgaa cgcatcatca agcgccagta gcgcagcttc
    21421 ctcggcgacg gcggctgcaa attctgcgaa ggcggccaaa acgtccgaga cgaacgccag
    21481 gtcttctgaa acggcagcgg gacagagtgc ctcagctgcg gcaggctcaa aaacagcggc
    21541 tgcgtcgtct gccagcgccg cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc
    21601 cgccggaaaa tcggcagaaa gcgccgcatc atccgcttca acagccacaa cgaaggctgg
    21661 cgaagccact gagcaggcca cggcagcagc gaggtcagct tccgcagcga agacatccga
    21721 aacgaacgcg aaagcgtcgg aaacccgtgc agaatcctca aaaacggctg ccgcatcgtc
    21781 cgccagttcg gcggcgtcat cggcatcatc ggcgtctgct tcaaaagatg aggcgaccag
    21841 acaggcgtca gcagcgaagg gcagcgccac gacggcatcc acgaaggcga cagaggctgc
    21901 tggtagtgcg acggcagcag ctcagagcaa aagtacggcg gaatctgcag caacgcgcgc
    21961 tgagacagcg gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag
    22021 cacgacgaaa aaggggatag tacagctcag tagtgcgact aacagcactt ccgagtcact
    22081 ggcggcaaca ccaaaagccg ttaaggccgc gtatgacctg gctaacggga aatacaccgc
    22141 acaggatgca acgacagcac agaaagggat aatccagcta agcagcgcga ccaacagcac
    22201 gtctgaaacg ctggcggcaa cgccaaaggc agtaaaagca gccaatgaca atgctgagaa
    22261 acgtctgcag aaagatcaga acggtgcgga tatccctggc aaagacacct ttacgaaaaa
    22321 tattggtgcc tgccgtgcct tcggtgggtc agtaagcaca acaacaggaa actggacgac
    22381 tgcacagttt atcgagtggc tggattctca gggagcattt aaccatccat actggatgtg
    22441 caagggttcc tggtcttatg gcaataataa aatcattact gatactggct gcggtaatat
    22501 tcatctcgcc ggagctgtca ttgaagtaat ggggataaag tcagcgatga cgatccgcat
    22561 taccactccg accacatcca gcggtggcgg aacaaccaac gcccagttta cctatattaa
    22621 tcacggaaca gattattcac ctggctggcg aagggactat aactccagaa ataagccaac
    22681 ggcatcagag atcggggcgc taccgtcagg tggaacagca gtatcatcag ttaatctggc
    22741 ttcaaaaggt cgggtagccg cgctgacaga caatacgcag ggggcagcag gtcttgagtt
    22801 atacgaggtg tataacaacg gatatccaac agcgtatgga aatatcattc acctgaaagg
    22861 gatgacagcc gttggcgaag gtgagttact catcggctgg agtggtacaa gcggtgctca
    22921 tgctccggca tttattcgtt cacgacggga tacgaccgac gcaaactggt cgccgtgggc
    22981 gcagctttac acctcggctc atcctcctgc agagttttat ccagtcggtg caccaatccc
    23041 gtggccatca gataccgttc cgtctggtta tgccctgatg caggggcaga cttttgacaa
    23101 atctgcttac ccgaaacttg cagccgctta tccgtcaggc gtgatccctg atatgcgtgg
    23161 ctggacgatt aagggcaagc ccgccagtgg tcgtgccgta ttgtctcagg aacaggatgg
    23221 cattaaatcg cacacccaca gcgccagcgc atccagtacg gatttgggga cgaaaaccac
    23281 atcgtcgttt gattacggca ctaaatccac gaataacacc ggggcgcata cgcacagtct
    23341 gagcggctct acaagttcag ctggggccca ccaacatagt cagactgggc ccagaacgaa
    23401 tagcgggagt caacctactg gaatgtttcc ggcagggagt actcaagtta gcgggactaa
    23461 tcaggttggt atcagtggct cattaaccag tggcacctcc caatgggtag gtaaatcatc
    23521 ttcagaagga aaccatacac actcactgtc cggcacagca gcatctgcgg gtgcacacgc
    23581 gcatactgtc ggtattggtg ctcatacgca ctctgttgcg attggttcac atggacacac
    23641 catcaccgtt aacgctgctg gtaacgcgga aaacaccgtc aaaaacatcg catttaacta
    23701 tattgtgagg ctcgcataat ggcattcaga atgagtgaac aagcacggac cataaaaatt
    23761 tataacctgc tggccggaac taatgaattt attggtgaag gtgacgcata tattccgcct
    23821 catacaggtc tgcctgcaaa cagtaccgat attgcaccgc cagatattcc ggctggtttc
    23881 gtggctgttt tcaacagtga tgagtcatcg tggcatctcg ttgaagatca tcggggtaaa
    23941 acggtttatg acgtggcttc cggcgacgcg ttatttattt ctgaactcgg ctcattaccg
    24001 gaaaatgtca cctggttatc cccggaaggg gagtttcaga agtggaacgg cacagcctgg
    24061 gtgaaggata cggaagcaga aaaactgttc cggatccggg aggcggaaga aacaaaaaac
    24121 agcctgatgc aggtagccag tgagcatata gcgccgcttc aggatgctgt ggatctggag
    24181 atcgcaacgg agaaagaaac cttgttgctg gaagcttgga aaaagtatcg ggtgttgctg
    24241 aaccgtgttg atacatcaac tgcaccggat attgaatggc cagtagcacc tatagggtaa
    24301 attcgtaatg attacctaaa tacgttattc ttttgttaaa aaagtgattt tgctataggt
    24361 agggagtatg ttcttggatt aagaataaaa aatcgccatg aaagagcttc atggcgagta
    24421 cataaacgta caaaatgtga tgaaagtaga gtggcgtaag ccactatatt aagatatgac
    24481 aatagcgaag aaaagtaaat aacattttat ggacgtttaa ttgttaatta taaaaactag
    24541 agtgcccgat attgtttatc ttgctttaaa ataatgtact caaaccaaat ttttgagcta
    24601 aaagctcaaa taatataaat gcaataataa tgtttaataa gacaattagc ttgctttgta
    24661 ttttcatttc ggaatactgt taagtaactt tgatgaccct aacaatagat cggaattttc
    24721 ttagagtcaa tggtcactgt ttttgcttgc aaagcaaatg ttttggtggt tataagtcgt
    24781 cttaatatag tcacgcatgc aacttgttcc atgcggagat taaagattgg aatataacgc
    24841 gaagatggaa ttaaattaca tttgcaataa ttaatttatt attactcaat aattaccgat
    24901 attattattg taaattaatc attagaatgc aaaaagaaat gtaacggcaa aataagagga
    24961 cgatagctga gaaaaaagcg tgctttccca atctggggca aatcacgtct gaatgacacc
    25021 tgaaaacagg tggta
//
LOCUS       DAJTVE010000054        24871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000054 DAJTVE010000000
VERSION     DAJTVE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..150
                     /locus_tag="OO439_002089"
     CDS             <1..150
                     /locus_tag="OO439_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316454.1"
                     /translation="IGYIFQNDIVALKQAFSLPDIDYADISQREQLAAALKRWPLLAE
                     FAQQK"
     gene            162..914
                     /gene="bcsQ"
                     /locus_tag="OO439_002090"
     CDS             162..914
                     /gene="bcsQ"
                     /locus_tag="OO439_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6316455.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            911..1075
                     /locus_tag="OO439_002091"
     CDS             911..1075
                     /locus_tag="OO439_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6316456.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1268..3529
                     /gene="bcsA"
                     /locus_tag="OO439_002092"
     CDS             1268..3529
                     /gene="bcsA"
                     /locus_tag="OO439_002092"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6316457.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3540..5878
                     /gene="bcsB"
                     /locus_tag="OO439_002093"
                     /pseudo
     CDS             3540..5878
                     /gene="bcsB"
                     /locus_tag="OO439_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            5885..6991
                     /gene="bcsZ"
                     /locus_tag="OO439_002094"
     CDS             5885..6991
                     /gene="bcsZ"
                     /locus_tag="OO439_002094"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6316458.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6973..10465
                     /gene="bcsC"
                     /locus_tag="OO439_002095"
                     /pseudo
     CDS             6973..10465
                     /gene="bcsC"
                     /locus_tag="OO439_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10547..12535
                     /gene="hmsP"
                     /locus_tag="OO439_002096"
     CDS             10547..12535
                     /gene="hmsP"
                     /locus_tag="OO439_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6316459.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12718..14004
                     /gene="dctA"
                     /locus_tag="OO439_002097"
     CDS             12718..14004
                     /gene="dctA"
                     /locus_tag="OO439_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6316460.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14224..15720
                     /locus_tag="OO439_002098"
     CDS             14224..15720
                     /locus_tag="OO439_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6316461.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15816..16745)
                     /gene="kdgK"
                     /locus_tag="OO439_002099"
     CDS             complement(15816..16745)
                     /gene="kdgK"
                     /locus_tag="OO439_002099"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6316462.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16977..17744
                     /gene="pdeH"
                     /locus_tag="OO439_002100"
     CDS             16977..17744
                     /gene="pdeH"
                     /locus_tag="OO439_002100"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6316463.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17814..19874
                     /locus_tag="OO439_002101"
     CDS             17814..19874
                     /locus_tag="OO439_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6316464.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20056..21378)
                     /locus_tag="OO439_002102"
     CDS             complement(20056..21378)
                     /locus_tag="OO439_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6316465.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21789..22802)
                     /gene="yhjD"
                     /locus_tag="OO439_002103"
     CDS             complement(21789..22802)
                     /gene="yhjD"
                     /locus_tag="OO439_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6316466.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22851..23750)
                     /locus_tag="OO439_002104"
     CDS             complement(22851..23750)
                     /locus_tag="OO439_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6316467.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24270..>24871
                     /locus_tag="OO439_002105"
     CDS             24270..>24871
                     /locus_tag="OO439_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6316468.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5823 a         6321 c         6943 g         5784 t
ORIGIN      
        1 ataggctata tcttccagaa tgatattgtg gcgttaaagc aggcattttc actgcctgat
       61 attgattatg ccgatatttc ccaacgcgaa cagttggccg cggcattaaa acgctggccg
      121 ttgctggcag agtttgcgca acaaaagtag gggattggtg aatggccgta ctgggattgc
      181 agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc cgcattagcc tggtcattac
      241 aaatgttggg agaaaatgtc ctggtggtcg atgcctgccc ggacaacttg ttgcgcctgt
      301 catttaatgt tgattttacc caccgtcagg gctgggccag agcgatgctg gatggccagg
      361 actggcgtga cgctgggttg cgctacacct cgcagctcga tttgctgcct tttggtcagt
      421 tatccattga agaacaagaa aatccacagc actggcaaac ccggctgagc gatatttgct
      481 ccggcttaca gcaactaaaa gccagcgggc gttaccagtg gattttaatc gacttaccgc
      541 gtgatgcctc gcagataacc caccagctgc tgagtttgtg cgatcactcg ctggcaatcg
      601 tcaatgtgga tgccaactgc catatccgac tgcatcagca agcgctgccg gatggcgcac
      661 atattttgat taatgacttc cgtattggca gtcaggttca ggacgatatt taccagcttt
      721 ggttgcaaag ccagcgccga ttactgccga tgctcattca tcgtgatgaa gcgatggctg
      781 aatgcctggc ggctaagcaa ccagtaggtg aatatcgcag tgatgcgctg gcggctgaag
      841 agatactgac gctggcgaac tggtgcctgt tgaactactc cgggctgaaa acgccagtcg
      901 ggagtgcatc atgagtatcc tgacccggtg gttgcttatc ccgccggtca acgcgcggct
      961 tatcgggcgt tatcgcgatt atcgtcgtca cggtgcgtcg gctttcagcg cgacgctcgg
     1021 ctgtttctgg atgatcctgg cctggatttt tattccgctg gagcacccgc gctagcagcg
     1081 tattcgcgca gaacataaaa acctgtatcc gcatatcaac gcctcgcgtc cgcgtccgct
     1141 ggacccggtc cgttatctca ttcaaacatg ctggttactg atcggtacat cgcgcaaaga
     1201 aacgccgaaa ccgcgcaggc gggcattttc aggtctgcag aatattcgtg gacgttacca
     1261 tcaatagatg aacgagctgc ctgagcgcgt tagccataaa acacagcatc ttgatgagaa
     1321 aaaagagctc ggtcatttga gtgccggggc gcggcggttg atcctcggta tcatcgtcac
     1381 cttctcgctg attctggcgt taatctgcgt tactcaaccg tttaacccgc tggcgcagtt
     1441 tatcttcctg atgctgctgt ggggtgtagc gctgatcgta cggcggatgc cggggcgctt
     1501 ctcggcgcta atgttgattg tgctgtcgct gaccgtttct tgccgttata tctggtggcg
     1561 ttacacctct acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc ttattctgct
     1621 cttcgctgaa acgtacgcgt ggattgtgct ggtgctcggc tacttccagg tagtatggcc
     1681 gctgaatcgt cagccggtgc cattgccgaa ggatatgtcg ctgtggccgt cagtggatat
     1741 ctttgtcccg acttacaacg aaaatctcaa tgtggtgaaa aataccattt acgcctcgct
     1801 gggtatcgac tggccgaaag acaaactgaa catctggatc ctcgatgacg gcggcaggga
     1861 agagtttcgc cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca ccactcatga
     1921 acatgcgaaa gcgggcaaca tcaacaatgc gctgaaatat gccaaaggcg agttcgtgtc
     1981 gattttcgac tgcgaccacg taccaacgcg atcgttcctg caaatgacca tgggctggtt
     2041 cctgaaagag aaacagctgg cgatgatgca gactccacac cacttcttct cgccggaccc
     2101 gtttgaacgc aacctgggac gttttcgtaa aacgccgaac gaaggcacgc tgttctatgg
     2161 tctggtgcag gatggcaacg atatgtggga cgccactttc ttctgcggtt cctatgcggt
     2221 gattcgtcgt aagccgctgg atgaaattgg cggcattgct gtcgaaaccg tgactgaaga
     2281 tgcgcatact tctctgcgtt tgcaccgtcg tggctatacc tccgcgtata tgcgtattcc
     2341 gcaggcggcg gggctggcga ccgaaagtct gtcggcgcat atcggtcagc gtattcgctg
     2401 ggcgcgcggg atggtacaaa tcttccgtct cgataacccg ctcaccggta aaggactgaa
     2461 gtttgctcag cggctgtgct acgtcaacgc catgttccac ttcttgtcgg gcattccacg
     2521 gctgatcttc ctgactgcgc cgctggcgtt cctgctgctt catgcctaca tcatctatgc
     2581 gccagcgttg atgatcgccc tattcgtgct gccgcatatg atccatgcca gcctgaccaa
     2641 ctcgaagatc cagggcaaat atcgccactc tttttggagt gaaatctacg aaacggtgct
     2701 ggcgtggtat atcgcaccac cgacgctggt ggcgctgatt aacccgcaca aaggcaaatt
     2761 taacgtcacc gccaaaggtg gactggtgga agaagagtac gtcgactggg tgatctcgcg
     2821 gccctacatc ttccttgttc tactcaacct ggtgggcgtt gcggtaggca tctggcgcta
     2881 cttctatggc ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt gggtgttcta
     2941 caacctgatt gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac aggtacgccg
     3001 atcgcaccgc gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg gtcacctctt
     3061 ctcgtgtacc gttcaggatt tctccgacgg tggtttgggg atcaagatca acggtcaggc
     3121 gcagattctg gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc aggaatacgt
     3181 cttcccgacc caggtggcgc gcgtgatggg taatgaagtt gggctgaaat taatgccgct
     3241 caccacccag caacatatcg attttgtgca gtgtacgttt gcccgtgcgg atacatgggc
     3301 gctctggcag gacagctacc cggaagataa gccgctggaa agtctgctgg atattctgaa
     3361 gctcggcttc cgtggctacc gccatctggc ggagtttgcg ccttcttcgg tgaagggcat
     3421 attccgtgtg ctgacttctc tggtttcctg ggttgtatcg tttattccgc gccacccgga
     3481 gcggagcgaa acggcacaac catcggatca ggctttggct caacaatgat gataacgcga
     3541 tgaaaagaaa actattctgg atttgtgcag tggctatgag gatgagtgcg ttcccctctt
     3601 tcatgacgca ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca gctgtagccg
     3661 cccagacgga acaaaatccg caggtggggc aagtgatgcc gggcgtgcag ggcgctgatg
     3721 cgccagtcgt ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt gcgcaaattg
     3781 cgccgccgcc gggcagcatg gtgctacgtg gcattaaccc gaacggcagc attgagtttg
     3841 gtatgcgcag cgatgaagtg gtgacgaagg cgatgctcaa cctcgaatac acgccatcgc
     3901 catcgttact gcctgtccag tcgcagttaa aggtttatct caatgatgag ctgatgggcg
     3961 gctgccagtg accaaagaac agttgggtaa aaaaacgctg gcgcaaatgc ccattaaccc
     4021 actgtttatt accgacttca accgtgtgcg gctggagttt gtcggccatt atcaggacgt
     4081 gtgcgaaaac ccggccagca ccacgctttg gctggatgtt gggcggagca gtggactgga
     4141 tctgacctat cagaccctga atgtgaagag tgacctgtca cacttcccgg tgccattctt
     4201 tgacccgcgc gataaccgta ctaacacctt gccgatggtc tttgcgggtg cgccggatgt
     4261 tgggctgcaa caagcatctg ccattgtcgc ctcgtggttt ggttcgcgtt ctggctggcg
     4321 tgggcagaac ttcccggtgc tctataacca attgccggat cgcaatgcca ttgtctttgc
     4381 aaccaacgac aaacggccgg acttcctgcg cgatcatccg gcggtaaaag ccccggtgat
     4441 tgagatgatt aaccatccgc agaatcctta cgtcaaactg ctggtggtgt ttggtcgtga
     4501 cgacaaagac ctgttgcagg cagcgaaagg tatcgctcag ggtaacattc tgttccgtgg
     4561 tgaaagcgtg gtagtgaatg aagtgaaacc gctgctaccg cgtaagccgt acgatgcgcc
     4621 gaactgggta cgtaccgatc gtccggtcac atttggcgaa ctgaaaacct atgaagaaca
     4681 gttacaatcc agcggtcttg agccagcagc gattaacgtt tcgctaaacc tgccgccgga
     4741 tctctacctg atgcgcagta ccggcattga tatggatatt aattaccgct acaccatgcc
     4801 gccggtgaaa gacagttcgc ggatggatat cagcctgaat aaccagttcc tgcaatcctt
     4861 caacctgagc agtaaacagg aggcgaaccg cctgctgctg cggattccgg tattacaagg
     4921 tttgctggat ggcaaaacgg atgtctctat tccggcgctg aaactgggcg cgaccaacca
     4981 gctgcgcttc gactttgagt atatgaaccc gatgccgggc ggttcggtgg ataactgtat
     5041 taccttccag ccggtgcaga atcatgtggt gattggtgac gattccacca tcgacttctc
     5101 gaagtattac cacttcatcc cgatgccgga tctacgcgcc tttgctaacg cgggcttccc
     5161 gttcagccgg atggcggatc tgtcgcaaac catcaccgtg atgccgaaaa cgcctaacga
     5221 agcacagatg gaaacgttgc tgaatactgt tggttttatc ggcgcacaga cgggcttccc
     5281 ggcgattaat ctgacggtga ccgatgatgg cagcaccatt cagggcaaag atgccgacat
     5341 catgatcatc ggtggtatcc cggacaaatt aaaagacgat aagcagatcg acctgttggt
     5401 gcaggcgacc gaaagctggg tgaaaacacc gatgcgccag accccgttcc ccggcattgt
     5461 gccggacgag agcgatcgtg cggcagaaac ccagtcaacg ctgacctctt ccggtgcgat
     5521 ggcggcggtg attggcttcc agtcgccgta taacgaccag cgcagcgtga ttgcgctgct
     5581 ggcagatagc ccacgcggtt atgaaatgct taacgatgcg gtgaacgata gcggcaaacg
     5641 cgccaccatg ttcggttcgg tcgcggtgat ccgcgagtcc ggtatcaaca gcctacgtgt
     5701 tggcgacgtt tattacgtag gtcatctgcc gtggttcgag cgcttgtggt atgcgctggc
     5761 aaaccatccg attctgctgg cggtgctggc ggcaatcagt gtgatattgc tggcatgggt
     5821 actgtggcgt ctgctgcgaa ttattagtcg tcgtcgtctt aacccggata acgagtaatt
     5881 gaagatgaat gtgttgcgta gtggaatcgt gacgatgctg ctgttggctg cctttagtgt
     5941 tcaggcagcc tgtacctggc ctgcctggga gcagtttaaa aaggattaca tcagtcagga
     6001 agggcgcgtc atcgccccca gcgacgcgcg caaaatcacc acctccgaag ggcaaagtta
     6061 cggtatgttc tttgccctgg cggctaacga ccgtgtagct ttcgataata ttctcgactg
     6121 gacgcagaac aatctcgctc agggctcttt aaaagaacgt ttgcccgcct ggctgtgggg
     6181 caagaaagag aacagtaagt gggaagtgct ggacagcaat tcggcctccg atggtgatgt
     6241 ctggatggcc tggtcgttgc tggaggcggg gcgtttgtgg aaagagcagc gttataccga
     6301 catcggcagc gcgttgctaa aacgtatcgc gcgggaggaa gtggtgacgg tgcctgggct
     6361 gggttccatg ttgttaccgg gcaaagtggg ttttgctgag gataacagct ggcgttttaa
     6421 ccccagctac ctgccgccga cgctggcgca gtatttcacc cgctttggcg cgccgtggac
     6481 cacgctgcgc gaaaccaatc aacgtttatt gctggaaacc gccccgaaag gtttttcgcc
     6541 agactgggtg cgctatgaga aagacaaagg ctggcagcta aaagccgaaa aaacattgat
     6601 cagcagctac gacgctatcc gcgtttacat gtgggtaggc atgatgcctg acagcgatcc
     6661 gcaaaaagcg cggatgctca accggtttaa accgatggcg acattcactg agaaaaacgg
     6721 ttatccgccg gaaaaagtgg atgtggctac ggggaaagcg cagggtaaag gaccgatcgg
     6781 tttttctgcc gccatgctgc cctttttaca aaaccgcgat gcgcaggccg ttcagcgcca
     6841 gcgcgtggcc gataactttc ccggcagcga tgcctattac aactatgtgc tgaccctgtt
     6901 tggacaaggc tgggatcaac accgtttccg cttctcgaca aaaggtgagt tattacctga
     6961 ctggggccag gaatgcgcaa attcacacta aacatattca cgctttccct cggtctggcc
     7021 gtcatgccga tggtcgaggc agcaccaacc gctcagcaac agttgctgga gcaagttcgg
     7081 ttaggcgaag cgacccatcg tgaagatctg gtgcaacagt cgttatatcg gctggaactt
     7141 attgatccga ataacccgga cgtcgttgcc gcccgtttcc gttctttgtt acgtcagggc
     7201 gatattgatg gcgcgcaaaa acagctcgat cggctgtcgc agttagcgcc gagttcaaat
     7261 gcgtataaat cgtcgcggac tacgatgcta ctttccacgc cggatggtcg tcaggcactg
     7321 caacaggcac gattgcaggc gacgaccggt catgcagaag aagctgtggc gagttacaac
     7381 aaactattca acggtgcgcc gccggaaggt gacattgctg tcgagtactg gagtacggtg
     7441 gcgaaaattc cggctcgccg tggcgaagcg attaatcagt taaaacgcat caatgcggat
     7501 gcaccgggca atacgggcct gcaaaacaat ctggcgctat tgctgtttag tagcgatcgc
     7561 cgtgacgaag gttttgccgt cctggaacag atggcaaaat cgaacgccgg gcgcgaaggg
     7621 gcctctaaaa tctggtacgg gcagattaaa gacatgcccg tcagtgatgc cagtgtgtcg
     7681 gcgctgaaaa aatatctctc gatctttagt gatggcgata gcgtggcggc tgcgcaatcg
     7741 caactggcag aacagcaaaa acagctggcc gatcctgctt tccgcgctcg tgcgcaaggt
     7801 ttagcggcgg tggactctgg tatggcgggt aaagccattc ccgaactaca acaggcggtg
     7861 cgggcgaacc cgaaagacag tgaagctctg ggggcgctgg gccaggcgta ttctcagaaa
     7921 ggcgatcgcg ccaatgcagt ggcgaatctg gaaaaagccc tcgcactgga cccgcacagc
     7981 agcaacaacg acaaatggaa cagtctgctg aaagtaaacc gctactggct ggcgatccag
     8041 cagggcgatg ctgcgctgaa agccaataat cctgaccggg cagaacgcct gttccagcag
     8101 gcgcgtaatg tcgataacac cgacagttat gcagtgctgg ggctgggcga tgtggcgatg
     8161 gcgcgaaaag attatcccgc cgccgaacgt tattatcagc agaccttgcg tatggacagc
     8221 ggcaacacta acgccgtgcg cgggctggca aatatttacc gccagcaatc gccagaaaaa
     8281 gctgaagcgt ttatcgcctc gctctctgcc agtcagcggc gtagcattga tgatatcgaa
     8341 cgcagcctgc aaaacgaccg tctggcacag caggcagagg cactggaaaa ccagggcaaa
     8401 tgggcgcagg cggcagcact tcagcggcaa cgactggcgc tggaccccgg cagcgtatgg
     8461 attacttacc gactttcgca ggatctctgg caggccggac aacgcagcca ggccgatacg
     8521 ttaatgcgca atctggcgca gcagaagtcg aacgacccag agcaggttta cgcttacggg
     8581 ctgtacctac gcttacgggc tgtacctctc tggtcatgac caggacagag cggcgctggc
     8641 gcatatcaac agcctgccgc gtgcgcagtg gaacagcaat attcaggagc tggttaatcg
     8701 actgcaaagc gatcaggtgc tggaaaccgc taaccgcctg cgagaaagcg gcaaagaggc
     8761 agaagcggaa gcgatgctgc gccagcaacc accttccacg cgtattgacc tcacgctggc
     8821 tgactgggcg caacaacgac gtgattacac cgccgcccgc gctgcatatc agaatgtcct
     8881 gacgcgggag ccagctaacg ccgacgccat tcttggtctg acggaagtgg atattgctgc
     8941 cggtgacaaa gcggcggcac gtagccagct ggcgaaactg cccgctaccg ataacgcctc
     9001 gctgaacaca cagcggcgcg tggcgctggc acaggcgcag cttggcgata ccgcagcagc
     9061 gcagcggacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt cgatggaaag
     9121 cgcgatggtg ctgcgtgatg gtgcgaagtt tgaagcgcag gcgggcgatc caacgcaggc
     9181 gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc gtccgcagga
     9241 taacgacacc tttacccgac tgacccgtaa cgacgagaaa gatgactggc tgaaacgtgg
     9301 cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca cccttgagca
     9361 cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc acactaccat
     9421 gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg atttcgtcaa
     9481 tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca actggggcac
     9541 ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg ccagcgtggc
     9601 ggtcggctgg cgaaatgacg tctggagctg ggatatcggt accacgccga tgggcttcaa
     9661 cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc tgggttacac
     9721 cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg ggcaaaaaga
     9781 ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg tggggctaag
     9841 tctgagctac gataaaggtg aagcaaacgg cgtctgggca tcgcttagtg gcgaccagtt
     9901 aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct attactataa
     9961 ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga tctggcatta
    10021 cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc cgcaggaata
    10081 cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt cgtgggagct
    10141 gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt atccgctgat
    10201 gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg atggcggcag
    10261 cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta cttccaactg
    10321 gtttgttggc acggcaattg atatccagca ggcgaaagat tacgcaccca gccatttcct
    10381 gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac cgccgcagcc
    10441 gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat gccgctgcga
    10501 tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc gcgtaagtcg
    10561 ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt tcgtttttat
    10621 tttttgcacc gttttgctgt tccatctggt ccagcagaat cgctataaca cggctacgca
    10681 actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt tgaaaggcga
    10741 tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg tcagccgtgc
    10801 cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc cagagcgccc
    10861 ggtgccgata atggttactc gcctgtttga gctaccggtt caaatctcgc tgggcgttta
    10921 ctcgctcgaa cgtccggcaa acccgcagcc aattgcctat ctggtactac aggcggattc
    10981 cttccgtatg tataagttcg tgatgagcac cctctcaacg ttagtgacca tttacttact
    11041 tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga ttttgcatcc
    11101 gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg ttggtcacca
    11161 actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc gcagttacaa
    11221 cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg cgatgcgctt
    11281 cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc aggttgtcgc
    11341 gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg atactgcggg
    11401 cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa aactcaaatc
    11461 ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg ctgtcattgc
    11521 caacggtgta caggaaccgt ggcacgcaat caccttaggt cagcaagtgc tcaccatcat
    11581 gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca ttggcgtggc
    11641 gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt ctgcggcatt
    11701 taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc agatggaagc
    11761 cgcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa atcatcagtt
    11821 tgctatttgg ttacagccac aggtcgagat gaccagcggt aaactggtca gtgcggaagt
    11881 gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct taatcgatcg
    11941 cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag agtcctgtcg
    12001 attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa acctctctgc
    12061 gctgcaactg atgcacccaa atatggtggc ggatatgctg gaactgttaa cccgctatcg
    12121 cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg acgaccctca
    12181 tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg cgctggatga
    12241 tttcggcatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt tgccaatcga
    12301 cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca gcatgattgc
    12361 tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag gcgtggagac
    12421 tgaagcacaa cgcgactggc tggcaaaagc gggcgttggt attgcccagg gcttcctttt
    12481 tgctcaccca ctccctattg aaatcttcga agagagttac ctggaagaaa agtagctacc
    12541 ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc aaacttttta
    12601 acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg gtgttatttt
    12661 aaggccgcag gtaccccata accttacaag acctgtggtt ttactaaagg acaccctatg
    12721 aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc cattggtatt
    12781 ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg cgacggcttc
    12841 gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt aacgggcatt
    12901 gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact gctttacttt
    12961 gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt cgtgcagcct
    13021 ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc ggtttacgcc
    13081 gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc ggcgagcgtc
    13141 attggcgcat ttgccagcgg taacattctg caggtgctgc tgtttgccgt actgtttggt
    13201 tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtcat cgaaagtttc
    13261 tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat tggtgcgttc
    13321 ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca actggggcag
    13381 ctgattatct gtttctatat tacctgtatc ctgtttgtgg tgctggtatt gggttcaatc
    13441 gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga agaactgctg
    13501 attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga caagatggag
    13561 aaactcggct gccgcaaatc ggtggtgggg ctggtcatcc cgacaggcta ctcgtttaac
    13621 cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca ggccactaac
    13681 agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct ttcttctaaa
    13741 ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct ctctgcggtg
    13801 ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt tatgtcagaa
    13861 gctcgtgcgc tgactaacct ggtcggtaac ggcgtagcga ccattgtcgt tgctaagtgg
    13921 gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc gccggatggc
    13981 aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct tccccgagtg
    14041 cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg agttcaggtt
    14101 tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag tgtttttaac
    14161 gtcatttaga ctgactgaca acgggcagtt ttttattacc aggatagttg atcaggggtt
    14221 cacatgcagg gcacaaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct
    14281 ggctatgtgc aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc
    14341 aacggtttac agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt
    14401 cgcctgctgg ttaatatcgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac
    14461 gccatccctc gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca
    14521 ttgtggcagc aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac
    14581 accacgctgt ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaagcgctc
    14641 tcttatctgg caaatgccac tggcaaattg accatcacac cagaaaccat caaccacgcg
    14701 ctgcaaagtc aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc
    14761 tatcgtctga aagggtcaac cttgttaggt cacgatcctg ccgatccgct gaaacaaccc
    14821 gttgaagcgg aaaaaattaa agatttctat cagaaatggt acaccccgga tgcaatgacg
    14881 ctactggtgg tgggaaacgt ggatgcgcgc tcggttgtcg accaaatcaa caaaacgttt
    14941 ggcgaactga aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc tccgctgcgt
    15001 gcggaagcgg tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg
    15061 gatacgccgt ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac
    15121 ctggcccgcg aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag taacagcaaa
    15181 gacatcggtc ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac
    15241 atcgaatcac caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg
    15301 aaggttcgcg ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa
    15361 ctggagctgc agaaactgtt tgccgcctat gcgcgggctg ataccgatat tctgatgggt
    15421 cagcggatgc gttcgctgca aaatcaggtc gtcgatatcg cgccggagca gtatcagaaa
    15481 ctgcggcagg atttccttaa cagcctgacg gtggagatgt taaatcagga tctgcgtcag
    15541 cagttgtcga atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac
    15601 atgaaagcgt tgcaggcggc ctgggatcaa atcatggccc catcgactgc tgctgccgcc
    15661 acctctgtcg ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa
    15721 tgcctgatgg tgatgagtta gcacaaccat caggtattta tggatgagct ggatagaggg
    15781 gtaacacttt atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg
    15841 ataatcgcgc cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgccgcg
    15901 ttttccgcac tgccgcccgt caaacatacc gccagataac cggcgctgaa agagtcgcca
    15961 gctgcggtgg tatcgatcac tttttctttc ggcagtttca ccgccggaac atccactaac
    16021 ccttcgccag caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc
    16081 acgcccgcgt tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg
    16141 tcttcatcgt ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ttggtacacc
    16201 tgctgtgtct cttctttgct ggcccacagg cgcggacgat agttattgtc gaaaatcact
    16261 tttccgccgt tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg
    16321 cttaagatcg ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg
    16381 caaatcgccg cagactgctc actctccagc cagaatttgg cggcggcttc gttccgccag
    16441 tagtagaacg tacgctcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggcagacgg
    16501 ttttccatcc gttgggtcag ggaagtatca acgttctcgc cgtgccaggc gtccagcatt
    16561 tgctggctaa aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga
    16621 tcgacctgac gggcgatata gacggaagtg ttcagggtat cgccgccgaa accgcgctta
    16681 acgtccgcgc ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg
    16741 gacatagtcg tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc
    16801 tggtcaacca tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa
    16861 gattccgttg tggagtgagg aaatttaccc cggccaggcg gtaaagttct gcccttgcgc
    16921 gccgataatc tttgtcgagt ccgggcagca tcacttttaa acacaggaca tctttgatga
    16981 taaggcaggt tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac
    17041 ggcgtttttg gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg
    17101 gacggttaat ggccgtggag ctattaacgg tggtcacgca tcccttgaac ccttcgcaac
    17161 gcctgccgcc ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga
    17221 aagagcagat tgatttgctg gcgcaaaaag ccgacttctt tgtagagcac gacctgctgg
    17281 catcggtcaa tattgatggc cctacgctca tcgccctgcg tcagcaacca aaaatcctgc
    17341 gccaaattga gcgtcttccc tggctgcgtt tcgaactggt ggaacacatc cgcctgccga
    17401 aagattcaac ctttgcctcg atgtgtgaat ttggcccgct gtggctggat gattttggca
    17461 ccgggatggc aaatttctct gcgctaagtg aagtgcgtta tgactacatc aaaatcgcgc
    17521 gagagctgtt cgtgatgctg cgtcagtcgc cggaaggccg cacactcttt tctcagcttt
    17581 tacatctaat gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa acaccggaag
    17641 agtggcgtga tgttcagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc
    17701 cggcaccgat ggaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg
    17761 ctgggctgga ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca
    17821 aggcaggcaa aataaccgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga
    17881 tcattttgat tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct
    17941 ctgcggagtt gaatcgtccg ttcgctatcc gtggcgatct gggcgtggtg tgggagcggc
    18001 aaaaacaaga aactggctgg cgcagctggg tgccgtggcc tcatgtacat gcagaagaca
    18061 tcattcttgg taatccaccg gatattcccg aagtcacgat ggtgcatttg ccgcgagtag
    18121 aggcaacgct ggcaccgctg gcgctgctga ccaaaacggt ctggctgccg tggttcaagc
    18181 tcgaaaagcc cgacgcgcgc ctgattcgcc tctccgaaaa gaacaataac tggacgttta
    18241 atcttgccaa cgatgacaac aaagacgcga atgcaaagcc gtcggcatgg tcgtttcggc
    18301 tggataatat tcttttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg
    18361 atctggaaat ttttgttgat ccgttaggca agccgctgcc gttcagcgaa gttactggat
    18421 cgaaaggtaa agcggataaa gaaaaggtgg gcgattacgt ttttggcctg aaggcgcagg
    18481 gacgttataa cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg ctggcgctgc
    18541 gtggcgaagg gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg
    18601 cttttgatgg cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat
    18661 tttctggcga ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc
    18721 cgccgtttga aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct
    18781 ttgattatcg cggttttaat gggcgaattg gcgatagcga tatccacggt tctctggtct
    18841 acaccaccgg aaagccacga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc
    18901 tggcggacct gggaccgttg attggcgttg attccgggaa aggggcagaa aagtcgaaac
    18961 ggtctgaaca gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg ctgccttatg
    19021 accgcttcga aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaagggcggc
    19081 gcattgagca tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa
    19141 atgctgacct gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc attgcgacga
    19201 atattcatct ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc
    19261 gactgaaact gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa
    19321 tgaacggtga cgcggaacta cgcggtagcg gtaactcggt ggcggcgctt ttaggcaaca
    19381 gtaacggcaa cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga
    19441 ttgttgggct gaatgtcggc aactacattg tcggtgcgat atttggtgac gatgaggtgc
    19501 gggtgaactg cgcggcggcg aatctgaata ttgccaacgg cgtggcgcgc ccgcagattt
    19561 ttgctttcga tactgagaac gcgttgatta atgttaccgg cacggcaagt tttgcttcag
    19621 aacagctgga tttgactatt gatccggaga gtaaagggat tcggattatc acactgcgtt
    19681 cgccgctgta tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa gccggaccgc
    19741 tgattgcccg tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg acggcggcgt
    19801 tactggcact gatctcacct tccgaagggg aggctaatca gtgtcggacg atattgtcgc
    19861 agatgaagaa gtgatgataa ttgccggatg tctgacatcc ggcaattaaa cagatttaga
    19921 tttttggcaa agtgtgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    19981 aaatccttgt gaattcaata tattgtatgg aaattcagtc ctgataagcg tagcgcatca
    20041 ggcttttcac acttattaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat
    20101 taacgtcaag ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagt
    20161 ctgcaaccag gctgcgatat acggtgcaac ggaagccccg agaatcgacg ctacgttgta
    20221 agagaatgat gctccggtgt aacgcacttc tgttgggaac agctctggta acagcgcgcc
    20281 catcggcccg aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag
    20341 aatcgggttg ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag
    20401 cgtggtgata attaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac
    20461 tggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg
    20521 cggcaggcca agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat
    20581 gatgtaaaac agcgtatagg ttgccagcat gatgaacgta cccagtacgg ttacgcgaac
    20641 atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt
    20701 ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagtac
    20761 cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcgt cggtcagtag
    20821 ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcccagctg
    20881 tgggaaggag ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc
    20941 cgcgccgccc cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag
    21001 cagcggggcg aaaataccaa tcgtggcata gcccggcagc agaccaatca ccacggttga
    21061 aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa
    21121 atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag
    21181 cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat
    21241 aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga
    21301 ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgctcgt ggtcgagtgt
    21361 tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc
    21421 acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa
    21481 aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata
    21541 aatagtggat aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg
    21601 ggttatgagt tggaatgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga
    21661 agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg
    21721 gcctacgggg agccaggccg aataaaatcg tatgccggat aaggcgttta cgccgcatcc
    21781 ggcctatttt aaggctgcgt tttccctggc attcgcgggt cgtctttata ttcggcggtg
    21841 gcaatccacg ccgcgcaaaa cagcgtcaaa cgggcgaaga agtagaaaaa tgccatcagc
    21901 cccagcacgg aaccaaatgc tgcgccagag ggggatttca tcagcgatgg cagggtgtag
    21961 gtcatcacga ttttaatcac ttcaaaacca atagcagcca gaaatgttcc gcgaatcagg
    22021 gcttttttgc gcggacggtg gcgcggtaac cgccagaaga tccagaagaa aagcagatag
    22081 ttggcgaaga tggaaatcgc caggccaatc aaccgccacg tcggtttcag ccactcaatg
    22141 ctgttcaggt gcagggcgct aataatcatt tgctgcgccg aaccggcaac cgaggtgatc
    22201 gaaagcgtca caatcagcgc aatcaacaaa ccaatcagcg aaataaaatc acgtagatat
    22261 tttacccaga acttctcctg atcttgcggc gagcgttccc agacatcgcg cgactgggcg
    22321 cgaatcgctt cacgcaggtt acccatccag ttgatgccgg aataaagcgc cacagccagg
    22381 ccgacaagcc ctacagtcgt acgctgctga acggcggtgt tgatggtgtt tttcaacgtg
    22441 gcggctagcg tcggatcgct gatgttttgc agaattttgt cgaagatatc ctgtagcaac
    22501 atcggatggg aggccagcac aaagccccct gcggcaaacg acaccatcaa aatcgggatc
    22561 atcgacaaaa atgagaaata ggtgatagcc gcaccaaact ggttacccag ccgatcgtta
    22621 aagcgttctg tcgcgcgaat caggtgcgca ataaccggtt ggcgttggac tttttcggca
    22681 gtggtggtga cggtttccag cgcctggcta actttgccgc ctttctcgct attatccaac
    22741 tgcttaatcg gctcgtgctc cagatcctgg gtgggacgtt tgatctcgtt ttcctgcgtc
    22801 atccgctctt ttgtccttct gttgtggtca ttcttaaaaa gtatagtcgg tcagtccagg
    22861 tactctttca ttacgccagc cagccactgc ataaacaggt ttacacgccg ggaaagctcc
    22921 cgacgctgcg gataaaccag cgaaagggag agcggctcgg cacggtagcc tggcaacact
    22981 tcaataagct gcccggcacg cagggcttcg cgcacggcaa tgcgcgggat ctgaataatc
    23041 cccagaccgg caagaccggc ggcgagatag ttttcgctac tgtttaccgt caacatgccg
    23101 ccagacttaa accactggac gccattaacg ctggcaacct caaaacctaa cggatgtaca
    23161 cccaggtgcg gtgtataacg caccattgcg tgtgaagtca gatcatcggg gctttgcgga
    23221 taaccaaagc gcgtcagata gtgcggactg gcacagttga ccatggtcag tttgccgagg
    23281 ggacgggcga taacgccatc ttccggtaac gcgccagtgc gtatcacgca atcaaaacca
    23341 tcatgaagaa tatctaccgg acggttatgg ctactcagtt ccagctcaat tcccggatgc
    23401 tgatagagaa attccgacag acgcggcagt aacaggtttt tcgcgattcc tgacgggatg
    23461 tcgatgcgta atttaccgct gatactggtg gcatcctgtt gaaacagacc gtccagttcg
    23521 ctgagattac tcaacacatc ttttgcccgt tgataatagg tcatgccttc tggcgtgagt
    23581 ttgacccgtc gcgtagtgcg ctggagaagc tgggtgccaa gttgatgttc cagtgcctgt
    23641 atctggcgcg aaacacttcc ctttggcaaa gcaaagaaat ccgccgcgcg ggaaaaactt
    23701 tccagctccg cgactttgat gaacaactgc attgcgtgaa ttttatccat ttctttgacc
    23761 gattgttgtt cacagcgaaa cagtaacgcg catatcgcat tgtttattgt tttttctaac
    23821 atctaatgcc gggtttcaca atgttgtcat gacgaatgtg gcgatttctc agcaggaaaa
    23881 gggcggaata gctttatgag aaaggtgaat ccccttaaac agagcgtatt tttcagtgat
    23941 tccctttaaa gtgaatagag gacgtattgg ctatttgtgc ttatttttgt ctcattgcca
    24001 tttgttattt tggcttgtag tcgcggaatt tcatttttaa ttgcatgcgt actaaccacc
    24061 aatataagga aatcctcaaa attgttttaa atagtgccct cgacatgtaa tttcaggcgt
    24121 ttagctgtaa ctatttatat cggtaactat tttctacgcg tcatctctgt agaatggcca
    24181 ccgccaaatg gcgaattctt attttttctc gcgggtaata cccttccact acttaagtct
    24241 ttttaacgta ttcaggtgga gattcagtta tgcaaatagt catgtttgac aggcagtcaa
    24301 tatttattca tggaatgaaa atcagtttac agcagcgtat tccaggagtg agtattcagg
    24361 gggccagtca ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta
    24421 tgctcgatgg cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc
    24481 agtttcctga ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg
    24541 aagttattca ttttaatgtg ctggccattg tgcctcgtga ttcaaccgtc gagacatttg
    24601 cgttggcggt aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga
    24661 cgccggaaaa ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa
    24721 ccatgttagc ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg
    24781 gaacggtaaa agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc
    24841 aggcggcaat gatgttaaat atctcctcct g
//
LOCUS       DAJTVE010000055        24734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000055 DAJTVE010000000
VERSION     DAJTVE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..776)
                     /locus_tag="OO439_002106"
     CDS             complement(<1..776)
                     /locus_tag="OO439_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6316469.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKY"
     gene            1343..1708
                     /locus_tag="OO439_002107"
     CDS             1343..1708
                     /locus_tag="OO439_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6316470.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1748..2404
                     /locus_tag="OO439_002108"
     CDS             1748..2404
                     /locus_tag="OO439_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6316471.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2471..3889
                     /gene="dcm"
                     /locus_tag="OO439_002109"
     CDS             2471..3889
                     /gene="dcm"
                     /locus_tag="OO439_002109"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6316472.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3870..4340
                     /gene="vsr"
                     /locus_tag="OO439_002110"
     CDS             3870..4340
                     /gene="vsr"
                     /locus_tag="OO439_002110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6316473.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4329..5250)
                     /gene="yedA"
                     /locus_tag="OO439_002111"
                     /pseudo
     CDS             complement(4329..5250)
                     /gene="yedA"
                     /locus_tag="OO439_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5423..6340
                     /locus_tag="OO439_002112"
     CDS             5423..6340
                     /locus_tag="OO439_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6316474.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6419..6601
                     /locus_tag="OO439_002113"
     CDS             6419..6601
                     /locus_tag="OO439_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6316475.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6772..8466
                     /gene="yedQ"
                     /locus_tag="OO439_002114"
     CDS             6772..8466
                     /gene="yedQ"
                     /locus_tag="OO439_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6316476.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8463..9278)
                     /locus_tag="OO439_002115"
     CDS             complement(8463..9278)
                     /locus_tag="OO439_002115"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6316477.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9576..9803)
                     /gene="yodD"
                     /locus_tag="OO439_002116"
     CDS             complement(9576..9803)
                     /gene="yodD"
                     /locus_tag="OO439_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6316478.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9966..10154
                     /gene="dsrB"
                     /locus_tag="OO439_002117"
     CDS             9966..10154
                     /gene="dsrB"
                     /locus_tag="OO439_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6316479.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10198..10821)
                     /gene="rcsA"
                     /locus_tag="OO439_002118"
     CDS             complement(10198..10821)
                     /gene="rcsA"
                     /locus_tag="OO439_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6316480.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11106..11891)
                     /gene="fliR"
                     /locus_tag="OO439_002119"
     CDS             complement(11106..11891)
                     /gene="fliR"
                     /locus_tag="OO439_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6316481.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11900..12169)
                     /gene="fliQ"
                     /locus_tag="OO439_002120"
     CDS             complement(11900..12169)
                     /gene="fliQ"
                     /locus_tag="OO439_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6316482.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12179..12916)
                     /gene="fliP"
                     /locus_tag="OO439_002121"
     CDS             complement(12179..12916)
                     /gene="fliP"
                     /locus_tag="OO439_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6316483.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12916..13281)
                     /gene="fliO"
                     /locus_tag="OO439_002122"
     CDS             complement(12916..13281)
                     /gene="fliO"
                     /locus_tag="OO439_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6316484.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13284..13697)
                     /gene="fliN"
                     /locus_tag="OO439_002123"
     CDS             complement(13284..13697)
                     /gene="fliN"
                     /locus_tag="OO439_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6316485.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13694..14698)
                     /gene="fliM"
                     /locus_tag="OO439_002124"
     CDS             complement(13694..14698)
                     /gene="fliM"
                     /locus_tag="OO439_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6316486.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14703..15167)
                     /gene="fliL"
                     /locus_tag="OO439_002125"
     CDS             complement(14703..15167)
                     /gene="fliL"
                     /locus_tag="OO439_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6316487.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15272..16399)
                     /gene="fliK"
                     /locus_tag="OO439_002126"
     CDS             complement(15272..16399)
                     /gene="fliK"
                     /locus_tag="OO439_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6316488.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16396..16839)
                     /gene="fliJ"
                     /locus_tag="OO439_002127"
     CDS             complement(16396..16839)
                     /gene="fliJ"
                     /locus_tag="OO439_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6316489.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16858..18231)
                     /gene="fliI"
                     /locus_tag="OO439_002128"
     CDS             complement(16858..18231)
                     /gene="fliI"
                     /locus_tag="OO439_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6316490.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18231..18917)
                     /gene="fliH"
                     /locus_tag="OO439_002129"
     CDS             complement(18231..18917)
                     /gene="fliH"
                     /locus_tag="OO439_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6316491.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18910..19905)
                     /gene="fliG"
                     /locus_tag="OO439_002130"
     CDS             complement(18910..19905)
                     /gene="fliG"
                     /locus_tag="OO439_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6316492.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19898..21556)
                     /gene="fliF"
                     /locus_tag="OO439_002131"
     CDS             complement(19898..21556)
                     /gene="fliF"
                     /locus_tag="OO439_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6316493.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21772..22086
                     /gene="fliE"
                     /locus_tag="OO439_002132"
     CDS             21772..22086
                     /gene="fliE"
                     /locus_tag="OO439_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6316494.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22420..22752
                     /gene="emrE"
                     /locus_tag="OO439_002133"
     CDS             22420..22752
                     /gene="emrE"
                     /locus_tag="OO439_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6316495.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22921..23472
                     /gene="ybcL"
                     /locus_tag="OO439_002134"
     CDS             22921..23472
                     /gene="ybcL"
                     /locus_tag="OO439_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6316496.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23482..24279
                     /locus_tag="OO439_002135"
     CDS             23482..24279
                     /locus_tag="OO439_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6316497.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6078 a         6122 c         6334 g         6200 t
ORIGIN      
        1 tcgtattttg caccaaatgt ccatgcttca gctgtatcac caccagcaaa tttgctgtat
       61 tcattgtagt gatacccgcc aagagctact tgagaattgg tacggtcaga gtttgagtaa
      121 gcggcaccta agctcaaacc gaagtcaaag tcatatgaag tggaaagacc aaaaccatca
      181 ccgttctgga agcgaacgtc ttcgtgacca tttttagtac cttcttgatc ttctcctgca
      241 ttgctgcctt cgttgttacc ttgatactgc aatgcaaagt tcagaccttc caccagaccg
      301 aagaaatcac tattgcggta agtagctacc ccatttgcac ggcctgccat gaagttatca
      361 gtccaggtat aggagtcgcc gccgaataca ggaagcatat ctgtccaggc ttcaacgtcg
      421 taaatcacac catagttacg accatagtca aaggagccca tctcgccgaa cttcagacct
      481 gcaaaagcaa gtttgacata agtattatca gaaccttcag tgtcgctagc ctttgttttg
      541 gtcagtgctt gaccataacc agttaactga tctgtaattt gggtctcacc tttgaagcct
      601 aactcgatga tagtttcatc gccgtcatcg ccagggttat cggaaaaagt atgacgagca
      661 tcaacttttc cgtacagatc cagtttattg ccatttttat tataaatttc agccgcattt
      721 gctgcgccag caactaataa cgctgggacc agcattgcca gaacttttct tttcattatg
      781 tattcccttg tgattataat cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa
      841 agcacatttg gatactattc tatgaagttc attttatttt aaagattgcg tgtaacaaat
      901 gtatttaaaa gatttcaaaa tgtttcttat ctatttgata taattcagaa ttatgaattc
      961 taaatataaa catccatgaa aattataaat gtatatcaat atgttatgag ttgaatggaa
     1021 agatagggag tatcaatgat tgttgcgcca aacggcaggc gtgatcacgc gcacattaat
     1081 gaaggtataa aaaaagccgg catcatgccg gcccaaaaaa tataaacaag acgataaaag
     1141 gctaataacg gggttatcat gacacagaaa tcaaaaaagg tataattgta ttcagtgcta
     1201 atatattgtt tgggtgaatt aataaaacta ttaccaagga tataaataaa tctttattgc
     1261 tgtttttcca gtaattattg ccgttgcgca aaatttatct atttgttcaa aaaatgattg
     1321 agtcttgact ggctcatcca atgtggaaaa atgtgacttt tatcacataa tagtactaag
     1381 tctgaatttt ccgggttatc tcaaaatgga atacggttcg acaaagatgg aagagagact
     1441 ctctcgcagc cctggcggca aattggcttt gtgggctttc tatacatggt gtggctattt
     1501 cgtctgggca atggcgcgtt acatctgggt gatgagccgg atccctgatg ccccggtgtc
     1561 gggatttgaa agcgatttag gctctaccgc gggaaagtgg ttaggggcac tggtcggatt
     1621 tttatttatg gctctggtcg gggcattgtt aggcagcatt gcctggtaca cccgacctcg
     1681 tcccgcacgt agcagacgct atgaataaaa actgctaagg tggcgactct ttggcaacgg
     1741 agcgcgtatg gacttacaac actggcaggc acagttcgaa aactggttga aaaaccatca
     1801 tcagcaccag gacgcggcac agaagctggc ggcagatgac gatgtcgata tgctggtgat
     1861 tttaaccgcc tgttattttc acgatattgt gagcctggcg aaaaatcatc cgcaaaggca
     1921 gcgttcttca atcctggcgg cagaagagac gcgtcgcctg ctccgtgaag agttcgagca
     1981 atttccggcg gagaaaatcg aggccgtttg tcatgccatt gccgctcaca gtttcagcgc
     2041 gcaaatcgcc cccttaacaa cggaagctaa aatcgtccag gatgcggatc ggctggaagc
     2101 cctgggggcg attggcctgg cgcgcgtgtt tgccgtttca ggggcattgg gcgtggcgct
     2161 gtttgatggt gaagatccgt tcgcacagca tcgcccgctt gatgataaac gctacgcgct
     2221 ggatcatttc cagactaagt tgctgaagct accgcaaacc atgcaaaccg caagaggcaa
     2281 gcagctggcg cagcacaacg cgcatttttt agtcgagttt atggcgaagc tcagtgccga
     2341 actggcgggg gagaatgaag gtgtcgatca caaggtgata gatgcgtttt cacccgcggg
     2401 ataagcgcgt ggccctaaat ggctgtaatt atgttaacct gtcggccatc tcagatggcc
     2461 ggttaaatct atgcaggaaa atatatcagt aaccgattca tacagcaccg ggaatgccgc
     2521 acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt aaaacgctgg tggcgcagct
     2581 taatggtgtg ggtgagaatc actggagcgc ggcaatttta aaacgtgcgc tggcgaatga
     2641 ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat ctgcaaacgt tgttacccaa
     2701 accatcggca catcatccgc attatgcgtt tcgctttatc gatctatttg ccggaattgg
     2761 cggcatccgt cgcggttttg aatcgattgg cggacaatgc gtgtttacca gcgaatggaa
     2821 caaacatgcg gtacgcactt ataaagccaa ccattattgc gatccggcga cgcatcattt
     2881 taatgaagat atccgcgaca tcaccctcag ccataaagaa ggcgtgagtg atgaggcggc
     2941 ggcggaacat attcgtcaac acattcctga acacgatgtt ttactggccg gtttcccttg
     3001 tcagccattt tcgctggctg gcgtatcgaa aaagaactcg ctcgggcggg cgcacggttt
     3061 tgcctgcgat acccagggca cgctgttttt tgatgtggta cgcattatcg acgcgcgtcg
     3121 tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa agtcacgacc agggtaaaac
     3181 gttccgcatc atcatgcaga cgctggacga actgggctat gacgtggctg atgcagaaga
     3241 taacgggccg gacgatccga aaatcatcga tggtaaacat tttctgccgc agcaccgtga
     3301 acgcatcgtg ctggtgggtt ttcgtcgcga tcttaatctg aaagccgatt ttactctgcg
     3361 tgatatcagc gaatgtttcc ctgcacagcg agtgacgctg gcgcagctgt tggacccgat
     3421 ggtcgaggcg aaatatatcc tgacgccggt gctgtggaag tacctctatc gatatgcgaa
     3481 aaaacatcag gcgcgcggta acggcttcgg ttatggaatg gtttatccga acaatccgca
     3541 aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat ggcgcggaaa ttttaatcga
     3601 tcgcggctgg gatatggcca cgggtgagaa agattttgac gatccgctga atcagcaaca
     3661 tcgtccacgt cggttaacgc ctcgggaatg cgcgcgctta atgggttttg aagcgccggg
     3721 agaagcgaaa ttccgcattc cggtttcgga cactcaggcc tatcgccagt tcggtaactc
     3781 ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt gagccaaaaa tcaaacaggc
     3841 ggtggcgttg cgtcagcaag aggcacaaca tggccgacgt tcacgataag gccactcgca
     3901 gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat tgagaagcgc ctcgccagtc
     3961 tgttaaccgg gcagggcctg gcgtttcgcg ttcaggacgc cagtctgccc ggaagcccgg
     4021 attttgtcgt tgatgaatat cgctgcgtga tatttaccca tggctgcttc tggcatcatc
     4081 atcactgcta tctgtttaaa gtgcctgcga ctcgaaccga gttctggctg gagaagatag
     4141 gtaaaaatgt tgaacgcgat cgccgcgata tcagtcgttt gcaggaactc ggctggcgcg
     4201 tactgattgt ctgggagtgt gcattacgtg ggcgcgagaa gctgacggat gaagcgctta
     4261 ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag cgcgcagatc gacacgcagg
     4321 ggattcattt actcgcttga tgcgtcctga ataactggcg caactacggg ttttgccggg
     4381 aagagatatt ttcccaacgt gaccagtacc accgcgaaga caattacgcc gagcgccagc
     4441 cattcaatct tcgacagcgt ctctccgccc agtcccgtac ccagcaagac cgcgaccacc
     4501 gggttaacgt aggcgtagct ggtggcgaga gccggactga cattacggat taaatacata
     4561 taagcgttga tggcgataat cgaaccaaac agcgccagat agccgaccgc aaggaagcct
     4621 gaaagggaag ggagcgccgt cagtttttca cctgcaatca tcgacgcgat cattaacacc
     4681 acgcctgccg ccagcatctc aatcgcaccc gccatcatcc ctacaggtaa ggtaatgcgc
     4741 gagccataaa ccgagccaaa cgcccagcta atcgagccga ttaaaatcag aatcgcgccc
     4801 cacggattgc cgcttaaatt tccaccgcta ttgagcatga tgattccggc aagcccaatg
     4861 gcgataccca cccattccag tttgcgcgtt ttaatgccaa acaggcggct gaagcaccag
     4921 ggtaaagagg ggcacggttg caaccacaac ggcggcgatg ccggaaggaa cattttgatg
     4981 ctcggcaacc gtcaccatgc cattaccgac agccagcaat aacaggccaa tcagcgcggc
     5041 attgagcagc ggacgtagcg gggggagctt gtgtccgcgc agtagcaaaa atgccatcaa
     5101 taaaataccg gctgccagga atcgaacgcc tgccatcatt aacggaggcc agctttccac
     5161 gccaatccga atgacaaaat aggttgagcc ccaaatgata tacaacgcaa acagcgcgcc
     5221 aaaaagcggt aacaactggc ggaaacgcat aatccctcac ggtggaaata aaaaggtggt
     5281 tcatagtaaa cgtgaaaatc attctgctgg cgagagatat aattgcactt gattgtaaaa
     5341 aaaatgttga cctgtgaagc aagtatcaga gctgccgttt ttgcttcata cttacaccct
     5401 tcaacaataa aaatgagagg gaatgctttt ggccgggagt agtttactga cgttgctcga
     5461 tgatatcgcc acactactgg acgatatctc cgtgatgggc aaactggcgg cgaagaaaac
     5521 cgccggtgta ttaggggatg acttatcgct caatgcgcaa caagtttcag gcgtgcgggc
     5581 caaccgggaa cttcccgtgg tctggggcgt ggcgaaagga tcgctgatta ataaagtgat
     5641 tctggtgccg ctggcgctga tcatcagtgc gtttatcccg tgggcgatta cgcctctgtt
     5701 gatgattggt ggcgcgtttc tctgctttga aggagtagag aaagtgctgc atatgctgga
     5761 ggcgcgtaaa cataaagaag atccggcgca gagccagcag cgtctggaga agctggcggc
     5821 gcaggatccg ctgaagtttg aaaaggacaa aataaaaggg gcgattcgta ccgattttat
     5881 attgtctgcc gaaatcgtcg ccatcacgct ggggattgtg gccgaagcgc cgttgcttaa
     5941 tcaggtgctg gtgctttcag gcatcgcgct ggtagtgacc gtgggcgtct acggtctggt
     6001 aggggttatc gttaagattg atgacctggg gtattggctg gcggaaaaat ccagcgcgct
     6061 gatgcaggca ttaggtaaag ggttattgat cattgcgccc tggttgatga aggcgttatc
     6121 gattgtcggc acgctggcga tgttcctcgt cggcggcggt attgtggtac atggtattgc
     6181 gccgctgcat cacgccattg aacatttcgc cgggcagcaa agtgcagtgg tggcgatgat
     6241 attaccgact gttttaaatc tgattcttgg atttatcatc ggcggcatcg tggtgctggg
     6301 agtgaaagcc gtagcgaaaa tgcgcggtca ggcacattaa tactcgtaca ggctattatg
     6361 caaaattcgc catcttcaac taaggtgaac aagtttcact cgaaaaaagg aggcgagtat
     6421 gagctttatg gttagtgagg aagttacggt taaagagggc ggcccgcgga tgattgtcac
     6481 tggatactcc agcggtatgg ttgagtgtcg ttggtatgac ggttacgggg tcaagcggga
     6541 agcttttcat gaaaccgagc ttgttccggg ggaggggagt cgttctgcgg aagaagtttg
     6601 aaagggtatc attaacacca ggccggatca cttccggcct ggtgtgccaa tgcctgcaat
     6661 gaatgatagt cgcgtcttat catgccacca ttataaaatt atcgctgttt tgcggtacgc
     6721 tatcgccagg ttatgccaga atcataaaaa agcaggttgg gagtcgtcag ggtgcagcac
     6781 gagacaaaaa tggaaaacca gagctggttg aaaaaactcg cacgccgcct ggggcctggt
     6841 catgtcgtta atctctgctt tatcgtggta ttgctttttt ccaccttgct gacctggcgt
     6901 gaagtggtag tgctggaaga tgcctatatc tccagccagc gtaatcatct ggaaaacgta
     6961 gccaacgcgc tcgataagca tttgcagtat aacgtcgaca aactgatctt tttgcgtaat
     7021 ggcatgcgcg aagctctcgt agcgccactg gatttcactt cactgcgtaa tgctgtaacc
     7081 gagttcgaac agcatcgcga cgagcacgcc tggcaaattg aactcaaccg acgacgcacc
     7141 ctgtcagtca atggcgtatc ggatgcatta gtcagcgagg ggaatctcct gtctcgcgaa
     7201 aatgaaagcc tcgacaatga aattaccgct gcactggaag ttggttactt gctgcgactg
     7261 gcgcacaaca cctcgtcgat ggttgaacag gcgatgtatg tctcgcgtgc cggattttac
     7321 gtttcgacgc agccgacctt gtttacgcgc aatgtaccaa cgcgttatta cggctatgtc
     7381 acccaaccct ggtttatcgg ccattcgcaa cgagaaaatc gtcaccgcgc ggtacgctgg
     7441 tttacttcgc aaccggagca cgccagcaat actgaaccgc aggttaccgt cagtgttccg
     7501 gtagacagta ataactactg gtatggcgtg ctggggatga gtattcccgt gcgtaccatg
     7561 cagcaatttt taagaaacgc catcgataaa aacctcgatg gtgagtatca gctctatgac
     7621 agtaagctga gatttttgac ctcttccaat cctgaccatc caacagggaa tatttttgat
     7681 cctcgtgaac tggccttgct ggcgcaggcg atggaacatg acacgcgggg cggcattcgt
     7741 atggacagtc gctatgttag ctgggaacgt ctggaccatt tcgacggtgt gctggtgcgc
     7801 gtccatacgc taagcgaagg cgtgcgcggc gatttcggca gtatcagcat tgcattaacc
     7861 ctgctgtggg cgctctttac caccatgtta ctcatctcct ggtatgtgat tcgccggatg
     7921 gttagcaaca tgtatgttct gcaaagctcg ttgcagtggc aggcgtggca cgacacctta
     7981 acccgtttat ataatcgtgg cgcactgttt gaaaaagccc gtccgctcgc gaaattgtgt
     8041 cagacgcacc aacatccttt ttctgtcatt caggtcgacc ttgaccattt taaagcgatt
     8101 aatgaccgct ttggtcatca ggcgggcgac cgtgttcttt ctcatgctgc cggattaatt
     8161 agcagttcct tgcgtgcgca ggacgttgcc gggcgggtcg gtggtgagga gttttgtgtg
     8221 attctgccag gcgcgagtct gacggaggct gcggaagtcg cagaacgtat tcgcctgaag
     8281 ttaaatgaaa aagagatgtt gattgctaag agtacgacga tacgcatcag tgcctcgttg
     8341 ggggtaagta gcagcgagga aaccggtgat tatgattttg aacaactcca gtcactggct
     8401 gaccgtcggc tttatctcgc taaacaggcc gggcgtaatc gggtatgcgc gagcgataac
     8461 gcttaacgag cggagaaaaa atggtccagc ccttcacgcc agccttccgg tccttcacgc
     8521 tgcgttcgcc agacgcgggt cggatcctca tcatgcagat gcaccccttc acggtttagc
     8581 cctttcacaa tcaccgcgta atccattacc tccagtaagg gcgcatcgtt tggcccatcg
     8641 cccaggccaa gtgtggttgg gcgtttgcct gataattgtt gataggtcgc gataatccag
     8701 ttggcggcct gatcttttcc ggcagaggca tccaggacgt gccagaagcg cgcaccctgc
     8761 ataaactgta agcccagttc gttcagacga gcggtaaatt gtgccatacg ctcgtcactg
     8821 tcgcgccaga ttagcgttac cgacgcctca tgcagctgcg tcagcgccgc ctggctacgg
     8881 cttaatcccg tccattcggc gatggttgca tcgtcgacat catcgaaagt cgtgaattta
     8941 aaatgttctt tctcgcgtag cgtatttaaa acctggctga tttcgccatg gctaatacct
     9001 gagatgatgc gtggaaaacc gtctatatcc tgccattgtt cagcaagctg gatcactgcg
     9061 ccattttcgg caatcagtgg taacccttgt agccccaacg ttttttgcaa gtacagcatt
     9121 tccgctgaag ttttactgct acagagaatg acgggaacat tcgcttcgcg taaacggctg
     9181 agccagaggg cagtcggttg ccagtcataa ctatgactgt ccagcagggt gccatcaaga
     9241 tcgctaaaaa ccagtagtgg ttgttgaatt gaaaacatgg tcgtgcagtt ctccccttac
     9301 caggatgatt ctcaaaacag cacaaataat caaagatgat tttttcggga gcgatcccgt
     9361 tgacgccaga gccaccaagg ggtgacgcac gtcacatttc tattcataag tagcgttaat
     9421 tattcatatc gcgaatattt tcttgtcagc gaaaaaaatt gcggataagg tgatgaacac
     9481 atcagatttc ctggtgtaac gaatttttta agtgcttctt gcttaagcaa gtttcatccc
     9541 gaccccctca gggtcgggat ttttttattg tgcattcaac gattcacttc agagacgctg
     9601 aagtcatgaa tatccagttc gaaggcatcc gccaattcac gccatgtatg atattcacgt
     9661 ccatcgacgc taacgtgttc agaatcgttc tggctgcgat gaacttcgct ttcgctaatt
     9721 tcattgatcg ccgccagcag ggcatcgaca tcgacgctga cctcacgttt tgcggtatcg
     9781 ctgtactctt ttgcggtttt catcatcttt cctcgcaacc gttactctgt tgttgccgcc
     9841 agtcgttaag tccgcctggc atctcttaag tatagaccgt cgagaaaatc agcactcgcg
     9901 cgcgcctgga gctgccagaa aaacgaaatt gttctacact ggcacaaagc cacaggagga
     9961 aaacgatgaa ggtgaatgat cgggtaacag tcaaaacgga tggcggtccg cgtcgtcctg
    10021 gcgtggtact ggcagttgag gagtttagtg aaggcacaat gtacctggtt tcgctggaag
    10081 actacccgct cggcatctgg ttctttaatg aagcagggca tcaggacggt atctttgtgg
    10141 agaaagcaga gtaatcttat tcagcctgac tggtgggaaa ccaccagtca gaatgtgtta
    10201 gcgcatgttg acaaaaatac cattagtcac attatccgtc agtcggacga catggtagat
    10261 aacctgttta ttatgtgttt tgatcttacg tttaatatta cctttatgcg atgaaacggt
    10321 cttggctttg atattcattt ggtcagagat ttgaatggtt ccctgacctg ccatccacat
    10381 tcgcaacata ctcgattcgg ttcggctcaa tgataacgtc ggcatattta aaaaagaggt
    10441 tatcgttgtc tcttttttca gaatatcgcc aaggatatcg tcgagagatt ccggtttaat
    10501 cgatttagaa cttatcaata atttttttct gaccaataga tattcatcaa aatgaacatt
    10561 ggcaattgcc ataaaaacga taaataacgt attgggatgt tgattaatga tgtgcttgat
    10621 gtgctgactg ttagaagcat catggatgaa acagtcctca ttaataaaca ccactgaagg
    10681 gcgctgtgaa tcacaagcta tggcaaggtc atcaacggtt tcaatgtcgt tgatttctct
    10741 ttttttaacc cctctactca acagataccc ggttaaacct agtcgggtgt aactacataa
    10801 atccataata atcgttgaca tggcataccc tcactcaatg cgtaacgata attcccctta
    10861 cctgaatatt tcatcatgac taaacggaac aacatgggtc acctaatgcg ccactctcgc
    10921 gatttttcag gcggacttac tatcccgtaa agtgttgtat aatttgcctg gaattgtctt
    10981 aaagtaaatg ttgcgatatg tgagtgagct taaaacaaat atttcgccgc aggagtatcc
    11041 tggaagatgt tcgtaggtat tatttttcgg ataatcctta ggataacatg ataaacgtta
    11101 cggaattata ttaatggcaa ttcactaata atatcagcca gcaaattaaa catctcactg
    11161 aataaatgtt cgcaaaaagg tgcaattaac ggcattaatg ccgccattaa agagataccg
    11221 acagtcagag ttaatggaaa tccaataaca aaaatggata attgcggggc catacgattc
    11281 agtaaaccta atgccagatt cagggtcagc agcagagtaa tgagcggtaa cgccagcatc
    11341 agcccgttaa ggaaaatcaa actccccgct ttggtgagtg ccagaaacgc attgctgttc
    11401 aacggttcgc caccaattgg cagggtgtga aaggtatcga ccagcagtga aatcaaccat
    11461 aaatgaccgt taaatgtcag gaacagcagt aacgccagca tatccatgat acgcgctaaa
    11521 acgggcatat tgagatggct ggccggatcg acaaacgtcg caaatgacag ccccatttgc
    11581 agaccgataa tttcgccagc ggttcgcaca gcggcaaagg caaattgcat ggtaaaacca
    11641 agcgcaatgc cgatcaggat ctgctgcacg gccagccaca gagcaaagaa cgaaaaaaca
    11701 ggaacatcgt tggcaggtaa tgatggggca atgacgaacg tgatcatcat tgccagcccc
    11761 agttttaccc gtttcggcac gctgcgttcg ctcagaattg gtgcggtaga aatgagcgcc
    11821 agcacgcgca gtaacggcca gaagtacagg cttaaccagg aaagccattg atcgcttgtc
    11881 acctgcatca ttgtacggcc tacccgatga tatacggcag gttagtgaac aaggtgcgga
    11941 cgtaatccag caacagattg agcatccacg gtccggcaat aataatggca ataaatacgg
    12001 cgatgatttt tggaataaac gacagcgtca tttcgttgat ctgcgtggcg gcctgcaaaa
    12061 tactgatgat aaggcccgtg accagcgcca ccagcaacag cggggcagcc agtgccagcg
    12121 cgactttcat cgcttcagtc cccatcatca tgaccgattc aggtgtcatt ttgcctctct
    12181 agctgtaaaa gctctgcgcc agcgaaccga ccagcaattg ccagccatcc accagtacaa
    12241 acagcatcag tttaaagggc agagcaatgg tggcgggggg gaccatcatc atccccaatg
    12301 ccatcaacac gctggcaatc accaggtcga taatcaaaaa aggaatgaaa atcgtgaagc
    12361 ctatctgaaa tgcggttttc aattcgctgg tcacgtaggc cgggagcaaa atgcgcatcg
    12421 gcacggcttc aggtccctgc aacgggccgg tattcgctag tctggcaaac aaccctaaat
    12481 ctgcctcacg ggtctgacgc agcataaact cacgtagcgg ctgcgcccct ttttccagcg
    12541 cctcctgcat tgatattttc tcttcgctga atggctggta cgcatcgaca taaattttgt
    12601 cgatcaccga tgacataata aaaaaggtca aaaatagtgc cagccccagc aatacctggt
    12661 taggtggcgc ggagggcgtt cccagcgcgt tacgcaataa accaaaaaca atgatgatgc
    12721 gggtgaaact ggtcatcatc agtaaaattg ccggaataaa cgtcaacgag gtgatgaaca
    12781 ccagcgtctg caccgggagc gaccagcttt gtccaccgcc aggcagcggc tggctggtga
    12841 tacccggcag ttgcgcgaag gcgaggggcg taaccagcca gagaaggaca ggtgcgacag
    12901 acaacaaacg acgcatcagg atctcccact acgcttaagc aaatttttca tgaccgactg
    12961 aaaatcggtc tgcggtatct cttctgttgg tgctgaaggg ggaagtttat gcagcagatt
    13021 gatttgccct gcggtaacgc cgagcaccag ccgtgcatct tccacatcga ccaccacaac
    13081 ccgttcacgc gcgcccagcg aagcactggc gctaattttc agaccgttaa cgccagtgcg
    13141 tttcggggca aatcccaacc gttttaccag ccaggcagca gcgaggatca gggcaataat
    13201 ggcgatgagt gcgccgctga cctgcagcag tggcgcagca gaaaccggcg tggaagattg
    13261 cacagtagcg tggttattca tcactaacgg ctcaggcgac gcattcgctc agacggagta
    13321 atgatatcgg tgatccgcac gccatattta tcggcaacga ccaccacttc gccctgggag
    13381 attaaataac cgttgatcag aatatccagt ggttcgcccg ccagaccgtc cagcgccacg
    13441 acggaccctt gcgtcagacg caacagctct ttgatggtca tccgcgtacg gcccagctcg
    13501 acggtcagct tgaccggaat atccattatc aggtcgatat cctgcaacgt tccgctgaca
    13561 tcaccaccgc caaattgctg gaacaccgcg tcggcagcgc ttttgctgct ggttgatttt
    13621 tgttcgctca acgcttcagc ccacagatcg tccattgcgc cgttgttgtc atcggccgga
    13681 ttattcatgt cactcatttg ggctgttcct cgttcagaga atttaaaatc gggttaatca
    13741 aatgttctat ccgtaacgca tactgaccgt tgagagtgcc gtactgactg gtcagcaccg
    13801 ggacgccgtc aacatgggcg atgatgccat cgggtttttc tatcggcagg acgtcgccgg
    13861 gtttcagttt taaaatctgc gacaggcgta gcgagatatc ggcaaagttg gcgaccagct
    13921 ccagctgtga atgctgcacc tggcgcacca ggttatcgcg ccagttctga tcttcattac
    13981 gcgagttttc cagcggcggg ttaaccaaca attcccgtag cggctcgatc atgctgaatg
    14041 gcagacagat attaaattcg ccggtcaggt tgccaatctc cacatggaac ggcgtgttaa
    14101 ccacaatgtc gttcggcgag gtggtgatat tggtaaattt cacctgcatt tccgaacgca
    14161 cgtactcaac ttccagcgga ttaatcgcct tccaggcgtc gctatagcct tcaagcgcca
    14221 gctttaacat gcggttgatg acgcgctgtt cggtatgggt aaactcgcga ccttccactt
    14281 tggtcgggaa gcgtccattg ccgccaaaca ggttatccac ggcgataaac accagactcg
    14341 gtgaaaacac caccagccct gtgccgcgca gcggtttcag atggataagg ttcaggttgg
    14401 tcggcaccgg caggttgcgg gcaaattcat ggtacggctg aatgcggatg gccccgacgg
    14461 ttatatccgg gctacgacgc agcaggttga acagccccat acgaaaatgg cgggcaaagc
    14521 gctcattaat gatttccagc gcctgcaaac gttcgcgcac aacccgtcgt tgggtattcg
    14581 gatcgtacgg acgaatgtca ctttcgccgc taacactggc tgtcggttcg tctttgactt
    14641 cgctgtcacc attcaacagc gcatcaattt cagcttgaga aagaatacta tcgcccatgt
    14701 cgttatcgca gaataaaagc ggtatacatc acgtcggtga catcctgttt cggttgcccg
    14761 gcaacaagcg gggtggaaag cgtggtttta atctcggcaa tcaggttttt cttgccttct
    14821 tctgtcgcca gtacggcagc atcctgacgc gaaaacagta acagcaaacg gctacggact
    14881 tccggcaaat actcactcag ccgcgagcgg gtagcttcat ctttgagtcg cagggttata
    14941 ccgatataaa gcacgcgatc cgcatcgccc aaattgaccg taaaggtatc cagcgcatag
    15001 aagaccggtg agggcacgac gcgatgctgc gctttgtctt cagcggcaac ctgatgcgaa
    15061 tgccagtagc tgtaacctgc gctggcacag gccgcgaggg taatgaatac cagaatcggg
    15121 agccaaagcg atcgcttgct tttcttgctt atcgcgtaat cagtcatgtg ttgcggtctt
    15181 cctgtgtcgc tactgcttat cggctaatta tcctgcgtct tgagcgcaac aaagcgggga
    15241 aaagacgcgg attacggtgc tacctctgac gttaggcgaa aatatcaacg ccgctgttgc
    15301 ctgttacgcg cccttgtaaa gagacgggaa ccggaagcgt atcgtcttct tcccccgcca
    15361 gaggttcatg gtttactgtg cgttggcttt gctgttgttg ggaagcggcc tgctgctgac
    15421 cactaaagct ttcgccactg atgttgcttt gccctaactg aatgccactc tcggccagct
    15481 gcgtacgcag taccggcagc gctgcttcca gggcggcgcg tacgtgctga tgcggcgaaa
    15541 ccatctggat ttgcgcctgg ttatcatcca ctttgaggga gatttgcact tcacctaaat
    15601 cctgcgggtg cagacgcaac tctgcacttt gttgtccctg gcgggtgaac agcgaaatat
    15661 gctggcttaa tgattgttgc cattcgtgag aacccagcgg tgcactcaag acaggcgcgg
    15721 cgacggtgag cagtggctgt gtctggtgtg gagtgattag cgggctggcg gcagcggtca
    15781 ccggtgaagg tgtgctgatg acttccgctt tactctgggc ttctgctacc agcggtgtta
    15841 atggctgagc tggcgtgccg ggggcatcat caggctgtgc tgttgtgagt tgcgcagaag
    15901 tcagttttgt gaagagcgtt ggtttctctg ccggtaacac ggttgacggc gcatcagtca
    15961 ctttgggcgt attgtcaaaa cccggcaaca tcgcaaaaag ggcgctcagg cttgcggtga
    16021 ggtcttcatt cagatcatcc gctttttcgt cttttgtcgt atttttgtca gccaccgcag
    16081 ccaacgtcat cgtatgagcg gtggttaacg gtgtcgatgt ggattgttcg acgttgatga
    16141 caggcagtgt ttcatccaca gggatcagta aatccgcttg ttgcgcgtcg gaaacaatat
    16201 cgctgatcag cagctcgcct tttgtcgtgg gcttatctgt tgccaccagc acctgggggg
    16261 ccgctttgtc ggtagttgtc tcgcttgcta atgcttcgct caacaacgcg agaaaatctt
    16321 gtgcagcatc gctggctttg ccgccaggca atgtggtggt gtcaacgtcg gcggtaatca
    16381 agggcgctaa gcgaatcatt caggtttcct catggcggcg cgctgggcga actcatccat
    16441 ctttttctga tcgaggcggt tttctgcaag cagtgccgcc gtggattgcc gttcctgcag
    16501 tgtctgccag gcctgcaaac gttgtttctt ttctcgccag ctgttcaggg caatgtcaac
    16561 tttctgcgtc cactgattaa gttgctggcg atgctgagta atggcttttt ccagcgtctg
    16621 gataaactgc tgatagttga tccagcggtt gctggtcatc ccggcactca tatcgctgtt
    16681 gaggttattg cgatattcat tctgataatc gatcagcatt ttgagctgtt cttccgcctg
    16741 ctgacacccg cgacgcattt cacccagcag gcgcgcggca tcctctacct ctttttctgc
    16801 cagatctttc agggtcgcca gcgcaccatg ttctgccatc taccgttatc tcctgggtta
    16861 tgacactgtc gggaaaatac gctccagccc ctggagagac gcttcccagt ccgcacgttc
    16921 aaaaatgcct tgttgtaaat agccctccag ctgcggccac agggcgatgg ctttatcgag
    16981 catcggatcg ctgcctttgg catacgcgcc gacgctaacc agatcgcggt tacgctgaaa
    17041 actcgacaac agctgtttga aggtgcgcac tcgcgcgtaa tgttgctcac tgatcaacgc
    17101 cgtcattgcg cggctgatcg acgcttcaat atcgatagcc ggatagtgcc cggcttccgc
    17161 cagtcggcga gacagcacaa tgtgaccgtc gaggatcgcc cgtgcggagt ctgcaatggg
    17221 gtcctgctgg tcatcgcctt cagtaagcac ggtataaaac gcggtaatcg agccgccgcc
    17281 gctaatgcca tttccggcac gctcgaccag tgccggtaat ttggcaaaca ccgacggtgg
    17341 ataacctttg gtggcgggtg gttcgccaat cgccagcgca atttcacgct gggccatcgc
    17401 gtagcgggtg agggagtcca taatcagcaa cacatgctga ccacgatcgc gaaaatcttc
    17461 ggcaatgcgc gtggcatagg cggcaccttg cattcgcaga agcggagaga catccgccgg
    17521 agcggcaatc accacggagc gtgcacgccc ttcagcaccg aggatattct caataaaatc
    17581 ttttacttcg cgcccacgtt caccaatcaa acccacgaca atgacatcgg cgcgggtgta
    17641 acgtgccatc atccccagca gcacactttt accaacgccg gacccggcaa acagccccat
    17701 acgctgccca cgcccaacgg taagcagggc attgattggg cgcacgccgg tgtccagcac
    17761 atgttcaatc ggtgtacgtt gcaacgggtt aaatggcggg gtaatcagcg caccggtttc
    17821 cgtcgtatcg ggggagggca ggccatcgag cggcttaccg ctgccgtcca gaacgcgacc
    17881 taataacgcc ggaccgagcg gcaactgctt gccgctttgc agtccttcag ccgaaatgtt
    17941 tttggcataa acccgcgcgc cgggcaggac accttccact tcctccagcg gcattaaaaa
    18001 cagccgttga ccgttaaagc caacgacttc gctttctact tcgtgcgttt cgctgccgtt
    18061 ctggcgttca atgacacagg ttgcgccgag cggcaattgt aatccggtgg cttccagcac
    18121 cagcccggta gcgcgggtta atcgcccata gcgacgtacc gcaggcaact gcgccatttt
    18181 ggcttcaaag ttatccagcg tggttagcca gcgagtcagg cgcgtggtca ttacaccact
    18241 cctggtgctg ccagacggca gagttcttgc cagcgagtgg cgacactggc gtcgagatcg
    18301 ccttcatcgg cggagacttt acagccgcca ggatggagtg tgggatcgcc ccgcaagcgc
    18361 cagccatgca aacttaaggt tgcgccgagc atatcatcca cacgttgcag atcatccggg
    18421 tgcacgcgca gctgtggttt accgctgaat aacggttctt gctgcaacaa ctgttggatc
    18481 tgtttgatca gtgccgagtt atccactgtt ggcgtctgac cgatcacctg acgtgccgcc
    18541 tccagcgcca tctgcatcag gcgcgaagca atcacactat caagcgcatc aagggtagtt
    18601 tgaaattcgc tgaccagttg ctgcatccgg gcatgaattg gcgcttgttg agacttcgcc
    18661 tctgccagac cttgctccag cccctgggcc agtccttcct gatagccctg ctcatgacct
    18721 tgctggcgac cttcggcaat ccccgcctga taaccttgct catgggcctg catttgcagt
    18781 tgcgccagtt gctgctcaag gctaggttcg gcctcttcaa tgatggtttc ttccggctcg
    18841 accatgggca caaactctgc ctgtggtggc gcgagatcgt ccggcgtcca ggttttccac
    18901 ggcagattat cagacatagg tatcctcgcc gctgccaatt accatctcgc cagtttcggc
    18961 aaggcggcgc acaatcagca gaatcgcttt ctgttcgttt tccacctgcg acagacgcac
    19021 cggaccacgg ttggcgagat cgtcgcgcag aatatcggcg gcacgctgcg acatattgcg
    19081 caggaatttc tcgcgcagtg gctgctcggc ccctttcagc gcgatcaaca gcgattcgga
    19141 atccacttcc tgcaacagac gctgaatgct gcgatcgtcg acatccacca gattttcgaa
    19201 caggaacatc tcgtcgataa ttttttgcgc cagctcgccg tcgaattcgc gcacggcggt
    19261 aataacggct tcttcctgct gagttttcat caggttgata atttcggctg ccgttctcac
    19321 gccgcccatt ttgctgcgct tgagattctg accgtcgagc aagccattca gtacttcggt
    19381 cagttccgcc agcgcggctg gctgcacgcc gccaaaggtg gcgatacgca acatcacgtc
    19441 gtggcgcaga cgttcatcga acaacgccag aatatcggcg gcctgcgcgc gcttcagatg
    19501 caccagaatg gtggcgataa tttgcggatg ctcatcgcga atcagatcgg cggcactctg
    19561 cggctccata aagttgagcg tttcaatacc gctggcggta tcgcgagttt cgagaatatc
    19621 ttccagcagg ctggcggcac gttcctcacc cagagctttg accaataccg aacgcagata
    19681 atcgttggcg ttgatattca gtgcggcaaa ctgttcagct tcttgctcaa actccgccag
    19741 cacataggtt agctgcttgt tggagatctg cgtgacgttc gccattgcag cgctcagggt
    19801 ttgcacttcg cgctgggaga ggtgcttgaa cacctctgcc gcccggtctt cgccaatggt
    19861 catcagcagg atgacgcttt tatcggtgcc tgtcaggtta ctcatgatcg ttatttatcc
    19921 actggcgaat gaccagcgcc accacgcgcg gatcgttatc agacatttca cggatacgct
    19981 ggctcatgac ttctgccccc agacgttggt tagcgcgtcg ttgttggagt tgttcgtctt
    20041 tgctcaggcg gacttccacc gcatcttcca cttcctcacg ggcctgcgcc tgttgctgta
    20101 cagctttcat cgcctcggcg cgacgtgtta actgcgggcg taccgctttc cgccacagca
    20161 gccacgccac cagcagtacc agcaaccagc gaccggcagc cagcaactga tcgataaacg
    20221 cttgctgttg ccagaacggc agttctccgc cgctttcgtc actgctattg aacggcgagt
    20281 taacgacatt gagcgagtca ccgcgttttt cagaaaagcc catcgcttcg cgggtcagat
    20341 cttcaatttg cttcatctgt tcgttgctga gaggcaatgg tttaccgtct ggcaaggttt
    20401 tgtaattcac cactaccgcg actgacagac gttgcacatc gcccacgttc attttggtat
    20461 gacgaatggt gcgatcgact tcgtagttac tggtttcatt ccgctgtgtg ctacgcggcc
    20521 cgctattgct ggtggtgctc gcctgctgct ggcggttatt ttgatttgcc ggaggcgtgc
    20581 tgattggcgc gttattcgca ggtgccggtt gattcgacaa cgcccccggt acgccgcccg
    20641 gataaccgga accgctttgc tcgctctcat taagctggcg tgaacgaagc gccgcatgag
    20701 attcatcacc gttagggcga tactgttctt ccgtttgttc tttactggcg aagtccagtt
    20761 gcgccgtgac ctgggcgtga atattaccgt taccaacaat aggcgacagg attgcttcaa
    20821 tacgccgctg aatacggcct tcgacatcgc tggcatattt caactgagcg tcattaagat
    20881 cgcgcccgct ggtattggac tgggttaaca gatgtccgcc ctgatccacc agcgtgacgt
    20941 ttcccggcgg cagaccagca acggcgctgg aaaccaggtg cacaatggcg ctaatttgcc
    21001 cttcatcgag tgcgcggccg ggtaacagat ttaccgtcac cgatgcagaa ggggattttt
    21061 gttcacggac gaataaagac ggtttcggca ttgccagatg tacgcgcgcc ccttttaccg
    21121 ggccgatagt ttcgatggta cgagaaagct cgccttccag cgcccgctga taattcacct
    21181 gttcgctgaa ctggctgata ccaaactttt cctgatcaag cagttcgaaa ccgaccgcgc
    21241 cgcctttggg caaaccttgt tgtgccaggc gcagacgcag ttcgtgaact ttatctgccg
    21301 gaacttcaat agcgccgctg gcttcgctga agcagtaagg aatattcatt tgcgtcagtt
    21361 ggctgacaat tgcgccacca tcctgatcgg aaagattgct gaataatgtg cggtagtcgg
    21421 gggctttcgc ccacaggatc agtgcgacca tgaccgccac tgccgcggaa ccggcaacaa
    21481 tcaatggaat tttcggattc gcacgcaggc gattaagcca ctcaagagat tttgtctggg
    21541 ctgcagtcgc attcatcgcg cacctcgtgg ctgattatgg acggcgttat tggcaaagtg
    21601 gaacaagact cactcccggg tcagcaaact cgaaaaattg acgctgccat tatttgtgct
    21661 ttcgtttttt tctatccgcc aataaacccg ttttttttgt tgctatttag cgcctttgtc
    21721 ttattgactt actggtaagc tttgctacca gaaattatcc gggagatgaa aatgtcagcg
    21781 atacagggga ttgaaggggt tatcagccag ttacaggcta cggcgatgag tgcgcgtgcg
    21841 caggaatcac tgccacaacc gaccattagt tttgccgggc agctgcacgc cgcgctcgat
    21901 cgcataagcg atacacaaac agccgcccgc acgcaggcag aaaaattcac tctcggtgaa
    21961 cccggcgtgg cgttaaacga tgtgatgacc gatatgcaaa aagcctcagt ttctatgcaa
    22021 atggggattc aggtgcgtaa taagctggtg gcggcttatc aggaagtgat gtccatgcag
    22081 gtgtaggttt tgtaacctgt tgttaattac atccgatctc accgcgtggg gcatggatgg
    22141 ggcaaactca ctcaatttct ggtttaaaat ggatacctga tcctgaaagt tttctgccat
    22201 ccacgatccg taaacgcgat aacatgtttt gtcttcgctt tgctcatagg aagagtgaac
    22261 tcttttgtta gcctggggtt ccttctgatc attattgttc ctgcctttat ctcttgccac
    22321 gccagagccc cctggataca catacaccag gatgaaaacg gtgagttgtg ttcaaactgt
    22381 agtacaattc tctccagtat gaacaggaaa gaatatgcta tgaaccctta tatttatctt
    22441 ggtggcgcaa tacttgcaga ggtcattggt acaaccttaa tgaagttttc agaggggttt
    22501 acacggttat ggccctctgt tggcacaatt atttgctatt gtgcatcatt ttggttatta
    22561 gctcagacac tggcttatat acctacaggg attgcttatg ctatctggtc aggagtcggt
    22621 atcgttctga ttagtttgct ttcctgggga ttttttggcc aacgactgga cctgccagcc
    22681 atcataggca tgatgttgat ttgtgccggt gtgttagtca ttaacttgtt gtcacgaagc
    22741 gcaccgcatt aaaaaataat catccttgag ataacaggaa tctgatttgt gcatattatg
    22801 gctaatgttc ggggcaactt tataattatg attgcatttg cgcgatcatt gcgccttttt
    22861 gcataatgag atcaatgcac atcaaataaa ataacgatag gtcacaacaa agaggttttt
    22921 atgaaaaaac ttatcgtttc aagtgtattg gcattcataa cattttctgc gcaggccgca
    22981 gcatttcagg tcactagtaa tgaaataaaa acaggagagc aacttacaac gtctcatgta
    23041 ttttctggag ttgggtgtga ggggggtaat acatcgccct cattaacctg gtctggtgct
    23101 ccagaaggca ccaaaagctt tgccgtaact gtgtatgatc cagatgcgcc tacaggcagc
    23161 ggatggtggc actggactgt tgctaatatt ccagcaacgg taacatattt gcccgctgat
    23221 gcaggaagac gtgatggtac aaaacttccg actggtgctg tccaaggccg aaatgatttt
    23281 ggctatgcgg gatttggtgg agcatgtcct cccaaaggag ataaacccca tcattaccag
    23341 tttaaaatat gggctctaaa aactgataag attcctgtgg attctaattc cagcggagcg
    23401 ttagttggtt atatgcttaa tgccaataaa attgcaaccg ctgagataat acccgtttat
    23461 gaggtaaagt aggataagag tatgctggca aaagatagga ctaacctaaa gattgaagaa
    23521 atacggatgc ataaacatca tgagattcac agggttaagc ctcttatgcc agcttcatgt
    23581 cgtatccgtc agggaaaaaa agttatcaat tgggagacgc atagtctaac tgttgataat
    23641 aatcagataa tattatttcc ctgtggatat gaattttata ttgcgaatta cccagaagca
    23701 ggtctttatc ttgcagaaat gctttattat cccattgatc taattgaaaa gttccaaaat
    23761 ctttatgcga taaccgatca aattcgtaac acgacaggtt tctgcttacc tcagaattcc
    23821 gagttaatat attgttggga gcaactaaaa acatctattt cccgaggctt ctcaacgcaa
    23881 attcaggaac acttagcaat gggcgttcta ctttcattag gagcacatca tgttaattgt
    23941 ttacttttat cagatagtaa acaatcatta ataagtcgtt gttataacct aatgctatcc
    24001 gaacctggaa caaaatggac agcaaacaag gtagcgagat atctctacat ttctgtttcc
    24061 acattgcatc gccgtctggc aagcgaggga gtaagttttc aaagtatatt ggacgacgtg
    24121 aggttaaata atgcgttgtc tgctatacaa acgacagtaa aacccatcag cgagattgcc
    24181 agggaaaatg gttacaagtg tccttctcgt tttactgaaa ggttccataa tcgttttaag
    24241 ataacaccaa gagagctaag aaaagcgtcc agagagtaaa agtattttat gaaggagcaa
    24301 cagcatcgat ttttattttg gaaaataaat acggcatact caacctattg gagcctgaac
    24361 aatccggtga cttctgcgct aatcggggac gtttataagc acatataatt gatactatct
    24421 tcctccatca caatttcaga aatgcacgtg tgattttttt gcatccggcg tttggactat
    24481 gtggagttca agagtgggaa agttacaaga ggttgagggg ataattaaag ggatgcttat
    24541 gacattagat atccggatag gtacggatat cgttgttatt aaatctagtt tatacgttaa
    24601 gatgcagcta cagcagaaat tgccactgtt aattttttca tcgtgagccc ttttatttga
    24661 actattattt aaaaatgatg tcactgcact acaaatattc atccaatcaa tgagattatt
    24721 tcaagatgta agtt
//
LOCUS       DAJTVE010000056        24438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000056 DAJTVE010000000
VERSION     DAJTVE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1543)
                     /locus_tag="OO439_002136"
     CDS             complement(<1..1543)
                     /locus_tag="OO439_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCR6316498.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFF"
     gene            complement(1540..1853)
                     /locus_tag="OO439_002137"
                     /pseudo
     CDS             complement(1540..1853)
                     /locus_tag="OO439_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2053..4011)
                     /gene="acs"
                     /locus_tag="OO439_002138"
     CDS             complement(2053..4011)
                     /gene="acs"
                     /locus_tag="OO439_002138"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6316499.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4405..5841
                     /gene="nrfA"
                     /locus_tag="OO439_002139"
     CDS             4405..5841
                     /gene="nrfA"
                     /locus_tag="OO439_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6316500.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5886..6452
                     /gene="nrfB"
                     /locus_tag="OO439_002140"
     CDS             5886..6452
                     /gene="nrfB"
                     /locus_tag="OO439_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6316501.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6449..7120
                     /gene="nrfC"
                     /locus_tag="OO439_002141"
     CDS             6449..7120
                     /gene="nrfC"
                     /locus_tag="OO439_002141"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6316502.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7117..8073
                     /gene="nrfD"
                     /locus_tag="OO439_002142"
     CDS             7117..8073
                     /gene="nrfD"
                     /locus_tag="OO439_002142"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6316503.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8093..9811
                     /locus_tag="OO439_002143"
     CDS             8093..9811
                     /locus_tag="OO439_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6316504.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9804..10187
                     /gene="nrfF"
                     /locus_tag="OO439_002144"
     CDS             9804..10187
                     /gene="nrfF"
                     /locus_tag="OO439_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6316505.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10184..10780
                     /gene="nrfG"
                     /locus_tag="OO439_002145"
     CDS             10184..10780
                     /gene="nrfG"
                     /locus_tag="OO439_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6316506.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11122..12435
                     /gene="gltP"
                     /locus_tag="OO439_002146"
     CDS             11122..12435
                     /gene="gltP"
                     /locus_tag="OO439_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6316507.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12514..13203)
                     /locus_tag="OO439_002147"
     CDS             complement(12514..13203)
                     /locus_tag="OO439_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6316508.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13297..15444)
                     /gene="fdhF"
                     /locus_tag="OO439_002148"
     CDS             complement(13297..15444)
                     /gene="fdhF"
                     /locus_tag="OO439_002148"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15025..15027),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6316509.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15642..17108)
                     /gene="mdtP"
                     /locus_tag="OO439_002149"
     CDS             complement(15642..17108)
                     /gene="mdtP"
                     /locus_tag="OO439_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6316510.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17105..19156)
                     /gene="mdtO"
                     /locus_tag="OO439_002150"
     CDS             complement(17105..19156)
                     /gene="mdtO"
                     /locus_tag="OO439_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6316511.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19156..20187)
                     /gene="mdtN"
                     /locus_tag="OO439_002151"
     CDS             complement(19156..20187)
                     /gene="mdtN"
                     /locus_tag="OO439_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6316512.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20206..20481)
                     /locus_tag="OO439_002152"
     CDS             complement(20206..20481)
                     /locus_tag="OO439_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316513.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20690..22675)
                     /gene="yjcS"
                     /locus_tag="OO439_002153"
     CDS             complement(20690..22675)
                     /gene="yjcS"
                     /locus_tag="OO439_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6316514.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            22928..23245
                     /locus_tag="OO439_002154"
     CDS             22928..23245
                     /locus_tag="OO439_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6316515.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23314..23643
                     /gene="yjdP"
                     /locus_tag="OO439_002155"
     CDS             23314..23643
                     /gene="yjdP"
                     /locus_tag="OO439_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6316516.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23790..>24438)
                     /gene="phnP"
                     /locus_tag="OO439_002156"
     CDS             complement(23790..>24438)
                     /gene="phnP"
                     /locus_tag="OO439_002156"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6316517.1"
                     /translation="QPCSGVVKFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDH
                     VQGLFPLRWGVGDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPL
                     PLNHSKLTFGYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAP
                     RNHCDLNTVLALNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5542 a         6413 c         6655 g         5828 t
ORIGIN      
        1 agaagaacca gatgccgagg aatgccacgg tgatagagaa cagcgccggg tattcatacg
       61 ggaagatggc tttttcgtga ccaaggatct gtacccaaat cgtcgggccg aggatcatca
      121 gtactactgc ggtaatcagc cccagccagc cacccatcat cgcgccacgc gtggtcagtt
      181 tcgaccagta catagaaagc agaatgatcg ggaagttaca gctcgccgcg atggcaaacg
      241 ccagccccac cataaaggcg atgttctgat tctcaaacag cacgccgagg ataatcgcaa
      301 tcacgccgag gatcagtacg gtgatttttg atacccgcag ctcttcacgt tcggtcgcgc
      361 cttttttgaa gacgttagcg tacaagtcat gcgaaaccgc ggatgcgccc gccagcgtca
      421 gacccgcaac caccgcgagg atagtggcga aagcaaccgc tgaaataaaa ccgaggaaca
      481 ggttgccgcc cactgcattc gccaggtgaa cggccgccat gttgttacca ccaatcagat
      541 ggcccgccgc gtctttatat tccggattcg caccaaccag catgatcgcg ccgaagccga
      601 taataaaggt cagaatatag aagtagccca taaacccggt ggcgtagaac acgctcttac
      661 gtgcttcgcg ggcatcgctg actgtaaaga agcgcatcag aatgtgcggc aagcccgccg
      721 taccaaacat cagtcccaga cccagagaga gtgcggagat cggatctttc accagcccgc
      781 ccggcttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcactg aacagattgt
      841 tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca ccgaacagca
      901 acagcacggc tttgataatc tgtacccaag tggtcgccag catgccgccg aacaggacgt
      961 acatcatcat cagcacgccg accagcacca ccgcaatgtg atagttaagg ccaaacagca
     1021 gctcgatcag tttacctgcg cccaccatct gggcgataag gtaaagcgcc accaccacca
     1081 gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacggtaa gaggccacat
     1141 cggcaaaggt gtagcgcccc aggttacgca gacgttcggc aatcaggaac aaaatgatcg
     1201 gccagcccac caggaagccc agcgagtaaa ttaagccgtc atagccggag gtaaacacca
     1261 gcgcggagat ccccaagaat gaggcggcgg acatatagtc cccggcaatc gccagcccgt
     1321 tctggaagcc agtgatattg ccgcctgcgg tgtagtagtc gctacgagaa cgtacgagtt
     1381 ttgatgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac ataataatcg
     1441 cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg gcgttagctg
     1501 cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt gtacctcatg
     1561 caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt agataccggt
     1621 gagaacaaag gagatcacaa tcactccaac accaattgga ataccgcgtg tgacgctggt
     1681 gttcggattc agcggcgtgc cagccagccg ggcgcgaagg cgatcagtaa aataaagccg
     1741 atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg tttttcgact
     1801 aactccctga aatgcgcatt gtcttctatc cgctgataaa tagtgccatt catcacagat
     1861 tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag aaggttaacg
     1921 ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca
     1981 atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg actcgcatcc
     2041 ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca gcttctcgac
     2101 tacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat
     2161 tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg agtcggtcca
     2221 gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt tgcggacttc
     2281 tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacgtagg cgtagatcgc
     2341 ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct tcggatgcgc
     2401 caccagcgcc gactcaatct ctgccgtccc cagacggtga ccggagacgt tcagcacgtc
     2461 gtccacacgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc cgtcgccgct
     2521 gaaatacata tttttgaagg tggagaagta ggtctgttca aaacgttcgt gatcgccaaa
     2581 cagcgtacgc gcctgacccg gccaggagtc ggtgatcacc aggctgcctt cggtagcgcc
     2641 ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga acggacgtgt
     2701 tgccgaaccg gctttcagct cggtagcgcc aggcagcggc gtgatcatga aaccgccagt
     2761 ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt ttttccagta
     2821 ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc gcagcgacga
     2881 acggtcggta ccctcgatcg ctttatcgcc ttccgccatc agcgcgcgga tcgccgtggg
     2941 tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac gggcaggcgt
     3001 cggccagttg ggtacgcctt caaacatcag cgtggtcgca ccgcaggcca gcgggccgta
     3061 cagcaagtaa ctgtgtccgg tcacccagcc cacatcggcg gtgcaccagt agatatcacc
     3121 cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat aaccgccggt
     3181 agtatgcagc acaccttttg gcttaccggt agaaccggag gtgtagagaa taaacagcgg
     3241 atcttcggcg ttcatctttt ccgcctggtg ctgatcgctc gcttgctcaa ccaggtcgtg
     3301 ccaccacagg tcgcgccctt cctgccagtc aattttcccg ccagtacgct tcagtaccac
     3361 cacatgctct acgctggtga cgttcgggtt tttcagcgcg tcatcaacgt ttttcttcag
     3421 cggaatactg cgcccggcac gcacaccttc gtcggaagtg atcaccagtc gtgagttgga
     3481 atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca ccgaatgcac
     3541 cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca tcggcatata
     3601 aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg cgaagcggca
     3661 gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt cgtcgccttc
     3721 ccagatgatg gcggtacgat cgccgttttc ttgcagatgg cggtcaaggc agtttgccgc
     3781 cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg caaaggaggt
     3841 gtttttcacc ttctggtaag gtttgatcca gtcaagaatt tttccctgtt cgccccagaa
     3901 ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag ggtttatcag
     3961 gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca tgcttttgtt
     4021 ctccttgtag ggtgttaata atatgtggca taagcgttaa atgtaggggt attggcagtt
     4081 ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata aacggcaggg
     4141 taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg cagttatgca
     4201 ttgctgttga aaaagaggaa gatactaact aactctaaag tggtatttta catgcactta
     4261 caattgatta aagacaacat tcacagtgtg gttatttgtt acacataggg gcgagcaatg
     4321 tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat aaaaaccgcc attgtaacaa
     4381 tggcgcaatt cggatgaagc ccctatgaca aggataaaaa taaacgcacg ccgtatcttc
     4441 agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac ggcagctccc
     4501 gcaaaacctg taactgtgga agcgaagaat gaaacctttg ccccgcagca tcccgatcaa
     4561 tatctctcct ggaaagccac ctcggaacag tcagagcgtg ttgacgccct ggcggaagat
     4621 ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa caagccgcgt
     4681 ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc gtaccggtgc gccgaaaaac
     4741 gctgaagatg gtccgctacc gatggcgtgc tggagttgta aaagcccgga tgtggcgcgt
     4801 ctgatccaga aagacggcga agatggctac ttccacggta agtgggcgcg cggcggcccg
     4861 gaaatcgtca acaacttagg ttgtgccgac tgccataaca ccgcctcacc agagttcgcc
     4921 aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat ggaagccatt
     4981 ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat cgatggtttg cggtcagtgc
     5041 catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg ggatgacggc
     5101 atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga ctggactaac
     5161 tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac ctggacagcg
     5221 ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc atatgccaaa agtgcagaac
     5281 gccgaaggca aactctatac cgaccataaa attggtaatc cgtttgataa cttcgcccag
     5341 acttgtgcga actgccatac ccaggacaaa gctgccttgc aaaaagtggt cgcggaacgt
     5401 aagcagtcga ttaacgacct gaaaatcaag gttgaagatc aactggttca cgctcacttc
     5461 gaagcgaaag cagcgctgga tgcaggcgcg acggaagctg aaatgaagcc aattcaggac
     5521 gatatccgtc atgcccagtg gcgctgggat ctggcgatcg cttcccacgg cattcatatg
     5581 cacgcaccgg aagaaggttt acggatgctc ggtacggcga tggataaagc ggcggatgca
     5641 cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca cccatgaaat ccagatcccg
     5701 gatatctcaa ccaaagagaa agcccagcag gccattggcc tgaacatgga acaaatcaag
     5761 gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagaaca ggcacgtaaa
     5821 aacggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa cacaatggag
     5881 tgaatatgag cgtattacgt tcgttattaa ctgccggggt gctggcgtcg ggcctgttgt
     5941 ggagcctgaa cggaattacc gctacccctg cggcgcaggc atctgatgat cgctacgaag
     6001 ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataaacca gataccgaag
     6061 gtatgcatgg caaacatgct tccgttatca acccgaataa caaactgccg gtcacctgca
     6121 ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat gtgatgcgct
     6181 ttaacgagcc gatgtacaag gttggagagc agaacagcgt ctgtatgtcc tgtcatctgc
     6241 cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca cgtaaccaaa gtggcgtgcg
     6301 ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt gacaaaggac
     6361 ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac tttaacccgg
     6421 cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt ttctcaccgg
     6481 cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc gtggtggcga aaacgttgaa
     6541 tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg gctgtaccgc
     6601 ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa ggcgtctcgc gcctgacgat
     6661 tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct tccgtaagtc
     6721 ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc ccgaccggtg cgtcttttcg
     6781 tgatgccgct agcgggattg tcgatgttaa tcctgatctc tgcgtcggtt gtcagtactg
     6841 catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga cggcggacaa
     6901 atgcgatttc tgccgtaaaa ccaatttgca ggccggtaag ctgcccgcgt gtgttgaggc
     6961 ttgcccgacg aaggcgctga cgtttggcaa tctggacgat cctaacagtg agatttcgca
     7021 actgctgcgc cagaagccta cttaccgcta caagctggcg ctgggaacca aaccgaagct
     7081 gtaccgcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc cgcatttcat
     7141 tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat tggtatttct
     7201 gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct acccgcaggc gggcggtgca
     7261 gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg gcgcggtgat cctcggtctg
     7321 ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat gttccactac
     7381 agttttacct cggtgatgtc gatgggggtg atgctgtttc agctctacat ggtggtgctg
     7441 gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg ccctgcaaca acgctggttg
     7501 ccgaagctgg ggatcgtgca aaaggttctg agcctgctga cgcccgttca tcgcggactg
     7561 gaaacgctga tgctggtgtt ggcggtgctg ctgggggctt ataccggctt tctgctgtcg
     7621 gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc cggtgctgtt cctcttctcc
     7681 ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca acgcagtaac
     7741 ccgcattcca cggaagcgca ctttgttcac cggatggaaa tccccgtggt atggggtgaa
     7801 atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg gcgatgacgg taaagttcgt
     7861 gcgctggtgg cggcattagg tggcggtttc tggacgtggt ggttctggct tggtgtcgcc
     7921 gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag ttccggcatt
     7981 cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg tgttgatgct gcgctttttc
     8041 attctctacg ccggacagtt gacggtggcg taagccagaa aagaggtggt ttctggacgt
     8101 attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca acgtgttgac
     8161 cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct gccatgatgc gactcacttg
     8221 catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac tgacgtattg
     8281 ttttctcatc agcgatttct cggtcattta tgtcgcgcaa catagctata gcctgctgtc
     8341 gtgggaattc aagctggcag cggtgtgggg cggccatgaa ggttcgctgc tgctttgggt
     8401 gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat tatcggcagc aaaccgatcc
     8461 gctatttccg ctgacgttag ccgttttatc tctcatgctc gccgcactgc tactgtttgt
     8521 ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag gccgcgatct
     8581 caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt accttggcta
     8641 tggcggtttg atggtagcgg cgagcgtggc gctggcgagt ctactgcgcg gcgagtttga
     8701 tgcggccagc gcccgaattt gctggcgctg ggcgttacct ggctggagtg cattaacggc
     8761 ggggatcatc ctcggttcct ggtgggccta ctgcgaactc ggctggggcg gctggtggtt
     8821 ctgggatccg gtggaaaatg cctctttatt accctggctt tctgccactg cgctgctgca
     8881 cagtttatcc ctgacacgcc agcgggggat tttttgccac tggtcgctgt tgctggcgat
     8941 agttactctg atgctgtcgc tgctgggcac cttaattgtc cgttctggca ttctggtttc
     9001 ggttcatgcg ttcgcactgg ataacgtccg cgccgtgccg ttgttcagcc tgtttgcact
     9061 gattagcctt gcgtctttgg ctctgtatgg ctggcgagcg cgggacggtg gcccggcggt
     9121 gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc tgttttgcgc
     9181 agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggtc tgctaggctg
     9241 gggacgcctc tccgttggcg cgccgtattt taaccgcgcg acgttaccgt ttggtctgtt
     9301 gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgcgc agcttccggc
     9361 gctggtcgct catgcgggcg tgctgttatt tgccgcaggg atcgtggttt ccagcgtcag
     9421 ccgtcaggag atcagcctga atttacagcc gggtcagcag gtgacgctgg caggatacac
     9481 cttccgtttt gagcgcctcg atctgcaagc caaaggcaat tacaccagcg aaaaagcgat
     9541 agtggcgctg tttgaccatc agcaacgcat tggtgaatta acgccggagc ggcgttttta
     9601 tgaagcacgc cgtcagcaaa tgatggaacc gtcaattcgc tggaacggca tccatgactg
     9661 gtatgcggtc atgggtgaaa aaaccggagc ggatcgttac gcttttcgtt tgtatgtaca
     9721 aagcggtgtg cgctggatct ggggtggagg attgttgatg attgcgggcg cattgttaag
     9781 cggatggcgg gggaagaagc gcgatgaata aagggcttct cacgctactg ctgttattta
     9841 cctgttttgc gcgcgctcag gtcgtagaca cctggcaatt cgccaatccg caacaacagc
     9901 aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat caaaacttac
     9961 tcgaatccaa tgcgccggtg gcggtcagta tgcgccatca ggtttatagc atggtgtcgg
    10021 aggggaagaa cgaagtcgaa atcattggct ggatgaccga acgctacgga gattttgttc
    10081 gctataaccc accgttaacg ggtcagacgc tggtgttgtg ggcgctgcca gtggtgttgt
    10141 tattgctgat ggcactgatc ctctggcgag tgagggctaa gcgatgaaac agcctcaaat
    10201 accggtgaaa atgctgacaa cactcacgat attgatggta ttcctctgtg tcggcagtta
    10261 tctgttaagc ccaaaatggc aggcggtacg tgcggagtat cagcgtcagc gcgatccgct
    10321 acatcagttt gccagccagc aaaccccgga agcgcagctt caggcattgc aggataaaat
    10381 ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg ggcgagtact atctgtggca
    10441 aaacgattac agcaattcgc tgctggcgta tcgtcaggcg ttgcaactac gtggtgagaa
    10501 cgctgaactg tatgcggcgc tggcgacggt gctttattac caggccagcc agcatatgac
    10561 cgcccagact tgcgcaatga tcgacaaagc cctcgcgctg gactctaacg aaatcaccgc
    10621 tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag ccatcgaact
    10681 atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc tggtcgagtc
    10741 gattaatatg gcaaaactgt tgcagcggag atcggattaa tcatctggtg atgaagatta
    10801 tgtcattgag ctgcataaaa aataatcgaa tgaacatatg ccaaaaataa tcactaatca
    10861 gtattattgc agattaaaca aataaaaatc tttccataac aaatggttat tcattaatcc
    10921 tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt taaagggttg
    10981 cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac aaaccaacgc
    11041 aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg ggaacggcgc
    11101 tccattgagg aagttattct gatgaaaaat ataaaattca gcctggcctg gcagattctg
    11161 tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag cgacagccgc
    11221 gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca tctgatcaaa
    11281 atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg tgttggtgat
    11341 gccaaacagc tcgggcgtat tggcgcgaaa accattattt acttcgaggt gatcaccacc
    11401 gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc cggggtggat
    11461 atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga agcggtacaa
    11521 agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa cattgtggcg
    11581 tcgatggcga aaggcgaaat gctgccgatc atctttttct cggtgctgtt tggtctgggg
    11641 ctttcttccc tgcccgcgac gcatcgtgaa ccgctggtga ccgtgttccg ctccatctct
    11701 gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg caccggtggg tgtgtttgcg
    11761 ctgattgcgg tgacggtggc taactttggt ttctcttctc tgtggccact ggcgaaactg
    11821 gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tagtgctggg aattgtggcg
    11881 cgcctgtgcg ggttaagcgt ctggatcctg attcgtattc tgaaagatga gctgattctg
    11941 gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa ttattgagaa gatggaagcc
    12001 tacggagcac cggcgtcgat caccagcttc gtggtgccga ccggttactc ttttaacctt
    12061 gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct gtatggcatt
    12121 gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac ctcgaaaggg
    12181 attgctggcg tgccgggcgt gtcgtttgtg gtgctgctgg caacgctggg tagcgtaggt
    12241 attccgctgg aaggtctggc gtttattgct ggtgttgacc gtatcctcga catggcgcgt
    12301 actgcgctga acgtggtggg taatgcgctg gcggtgctgg tgattgccaa gtgggaacac
    12361 aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag tgctgggcaa atttgataaa
    12421 actgcggatc aataattgaa gattgccggg gatatccacc cggcaatgtg tgaatgcctg
    12481 atgcgacgct tgccgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
    12541 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
    12601 cttgttcttc tcgataaaac cttcttcacc gtttaagaac atcattcccg cccagtactc
    12661 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
    12721 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
    12781 ggcagagtcg ttctccagat cttccgaggc gttttctaac agcgaaatag cctttggata
    12841 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
    12901 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
    12961 cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc
    13021 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
    13081 aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
    13141 ctcggagtcg ttggcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
    13201 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
    13261 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
    13321 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc
    13381 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
    13441 gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
    13501 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
    13561 cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
    13621 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
    13681 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
    13741 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt tcggggtatc
    13801 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
    13861 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
    13921 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
    13981 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
    14041 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
    14101 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc
    14161 ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc
    14221 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
    14281 gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc
    14341 tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
    14401 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
    14461 acccgcatgc ggcttaccga ggttaccagt cagcatcgcg aggctggtca gagaacgcac
    14521 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
    14581 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc
    14641 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
    14701 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
    14761 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
    14821 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
    14881 tacgtgattc gccacgattg ggtgggaatc agccgggttg tacccgaaaa cgaacactaa
    14941 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
    15001 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
    15061 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
    15121 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
    15181 ggtaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg
    15241 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
    15301 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
    15361 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
    15421 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
    15481 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
    15541 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
    15601 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
    15661 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
    15721 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
    15781 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
    15841 cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
    15901 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
    15961 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
    16021 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
    16081 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc
    16141 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
    16201 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
    16261 ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc
    16321 agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
    16381 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
    16441 ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
    16501 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
    16561 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
    16621 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
    16681 gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
    16741 ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa
    16801 ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg
    16861 agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt
    16921 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc atcggccagt
    16981 ttgatttgtt ccggtttgag ctgttgatga ggcgcagaat ccttgcgtac caaggcacag
    17041 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
    17101 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
    17161 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
    17221 ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
    17281 ttgccagacc ggcagcgtat ttttccaggg cgtcggcaaa ctgacctgct ctgtccggct
    17341 gtagtgcgtt atccagcgcc tgcgccgaat tccaggtggc atcccaggcg tgaaggatat
    17401 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
    17461 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
    17521 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
    17581 tccgcattac tttacttaac atacccagcg cgccagccag tttttgcggc agcgtgcgcg
    17641 cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga
    17701 tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga
    17761 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
    17821 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
    17881 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
    17941 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt ggattagaga
    18001 cgatcacgca ggtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg
    18061 tgtaacagat caaacaggcg agcaatgttt ttaccgcgta gcgtatatag tctggattgg
    18121 taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt
    18181 tcatctggcc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
    18241 ccaccgcttc actttcactg atccgccagt cgctttgcca gcactgacct tcagcaacgg
    18301 catgctgcag cttgttgatt tctgaggcta atttttgccg aaattcaata attgcctgag
    18361 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
    18421 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
    18481 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
    18541 cgaggctgtc tgtcaggtga ctaatggcat catcaagccg atcattaagc gcctgatgca
    18601 tttgcgaaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
    18661 gatagaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga
    18721 gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacagcgaaa aagaccagtc
    18781 ccagccgatg ggtgcgcatc aaaaacatgc ggcccatcag gatcggcccg gcgatgatca
    18841 atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca
    18901 gcaccgtggc aaccacaaac aagatcgcca caaatttggt gtaaaacgcg ttcgactgaa
    18961 taccgtaaaa cagcactgcc agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga
    19021 tcagaatcac cagcaggcag cctacccaga gttgcacgat ctgcggcacg cggccgggtc
    19081 gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccatcggt aatggcaggg
    19141 agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa
    19201 catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac
    19261 ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg
    19321 tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat
    19381 caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt
    19441 ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc
    19501 aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt
    19561 cgccatttcc agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgttgcgc
    19621 caccagcgca tcgacgccgc tgacggcgct ggcggctgac tgcgcctgta acaatacggc
    19681 attaagatcg gcttctgcgg cgcgctgcgc agttcttgca cggtcaacgt cttccgctga
    19741 gacaaaaccc tctttcagta atggctcggt acggcgtaat gtatctgtgg cctgtttcgc
    19801 ggcggcacgg gctttttcta ccgtggcatt aatcgagtcg gcaccaaact gttgcgcatc
    19861 aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt
    19921 cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac
    19981 tgcctggttg tcggtgaccg ccagttctac aatgcggccg ctgacttccg gcaccacatc
    20041 aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat
    20101 aacgaaaaca agggcaacca gcgccaatgc aaccactaac agagcaggga atttactgcg
    20161 aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag
    20221 ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag
    20281 attgatattt gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat
    20341 ggcgcagaaa aaccagctgg gataataagc accgatcacc gggattgccg gagataatga
    20401 gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat
    20461 gcgagagaga actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt
    20521 gaatatcaat atccattgaa ggcgtcagta tggctatttt ctcgatgaat aaaaatagct
    20581 ttattcagtc accgcattca taacttatat attttattta ttaattggct gtatatattt
    20641 ttaaaataaa tgcttttggg ggcttcagcg cccccaaaag cgagtgacat taatttgggg
    20701 ttacgatatt cacccacaaa tcgaaattat ccagacaggc gataatttct tccagtttcg
    20761 cgccattgcc aataattttg gctttcttcg cttttaccag atccgccatt ttggcttgcc
    20821 cggtcagcac ggcgtgcaga tcttcacggc tgatgtagaa agaggcgtca gcttgggatt
    20881 gcagtgtttt acggtagtta agcacgctat cgttcagcgt caggttgagg ttatcgccat
    20941 tgctcatatt gaagttcagg ctgatatttt tacccgcggc tttcgcgcta tcgaggcgaa
    21001 cggacataaa gtcgaacagc atttcgaccg acatcccgcg aatggtgtcc ggggaaccga
    21061 tggtgccgtg gctgaactta tgcacccctt cgcgcagctc tttcgcgccg gtcaggtaga
    21121 aaccgcgcca ggtggcggac tcggcctgat agcccagctg ttcaaagtta ttcgcttgca
    21181 gattcttcgc gacctggtca cccgggttgg cggcaatcac ctgtttcagc agttctgccg
    21241 accagcggta atcaccttgc ttgttcgctt cttgcgccag gttgatgaca cgggcagaac
    21301 cgcccagcgc ctgcacgtaa cgtttaccca tctccacctg accatacgga tgcaggttag
    21361 ccgggttacc gtcgtaatag ccaagataga agttatacac cgcgcgggcg ttgtggctga
    21421 cagaaccgta atagccacgg ctggcccagt tattggcaag tgcaggcggc agtttaatca
    21481 tgtcgccgat ttcattcatg gtgtagccct ggttcgccag gtgcagggtc tggtcgtgaa
    21541 tgtacttgat agtatcgcga tatttaccaa tataatcatt gatatgctta ttgccccaga
    21601 ccggccaggt gtgcggcata aacaggactt ccgcgtcgtt accccacata tccagcgttt
    21661 cgttcagata ctcggtccac ttgctggtgt cgcgggtttt cgcgccgcgc agagtgtaga
    21721 agttgtgcag ggtatgcgtg gcgttctcgg cggtacacag cgctttcagg gccggaatat
    21781 agaagtgcat ttcggctggt gcttcgctac ctggggtcat caggaagtca aactccaggc
    21841 cgtcgataat catcttctca ccggttttgg tgatggtttt gttcggtgcg atgatgctcg
    21901 ggtagcccga tgccagcgtt acgccaagac cattgccgat gttaccctga gggttatgcg
    21961 ccagcagcag gccgtaggag taaagtgcac ggcggctcat aatattaccc gccagtacgt
    22021 tttcgctgat ggcttcgtcc ataaagccag caggggcgat aacctgcact ttaccggatt
    22081 tcacatcagc ttcagagata atgcctttca cgccgccata gtggtcggta tggctgtggg
    22141 tataaataac cgcaacaatc ggtttttgcg ggcgattttg gaagtaaagg tcaagggcgg
    22201 cttttgcgct cggtggcgtc accagcgggt cgatgacaat aatcccggta tcaccttcga
    22261 taaaggtgat gttggagata tcctgaccgc gaacctggta cattctgtcg gtgactttaa
    22321 acaggccgga aataccgttt aactgcgact gacgccacag gctagggtta acggtttcgg
    22381 gcgcgggcgc attgatatca aatttataat cttccccgcg ataatatggt ttgccattag
    22441 catcgcgcag aataccttca tccagcagcg gagcgataaa tccacgctga gcgtcgtcaa
    22501 aatcctgacg atcgctaaac ggtaatgatt tggcgtaatt ctgattaacc tgttgggtat
    22561 attgagtggc ggctttcgct tcctctttcg cgttagcggt atttaccatc atgccgctgg
    22621 cggcagttaa cgcaataaca attctgctca aacggaataa ccgagagtta ttcatgtaaa
    22681 tctccattta tcatttatgt cgtaaatatg taatccatgc aaaatacgtc atttaaagct
    22741 ggggttatca taattgggaa tttgcgactt taataagtgg aagtgtgagc tgaacgatcc
    22801 attttattag gcatttgtgc tgaagaggga aaattacgcg aatttattat ctctgtaaat
    22861 aacatgaggt gttttatata tttattatat atattgtact tcggcaaata ctgagcacaa
    22921 tttatcaatg caaccggtgt ttctttttaa aaaacgtacc ggagcaatgg gcggtcatgt
    22981 cgctggcacc gcaggattct gcgttgctgt gccagccttt tattcatggt caatgagtga
    23041 ggtgattgat aaagacacca ggtcgtcaga gcgcactgat tatccgcatt atgccagtga
    23101 agttgcactg gcttttggtt cacgagtggt tggcctcgaa ctggcaaaaa tgattgtgga
    23161 tgcgtggctg ggcgcacagt acgaaggcgg tcgtcatcaa caacgcgtgg aggcgattac
    23221 ggcaatagag cagcggagaa attgagattc atccactact tgcatggatg agtaatgact
    23281 aatgtggata gagtttcttt ttgaggttgg ctaatgaaac gctttccgct atttctttta
    23341 ttcaccctgc tcacgctgtc caccgttccg gcccaggctg atattatcga cgatactatt
    23401 ggtaatattc agcaggcgat taatgacgct tataaccctg accgtgggcg agactatgaa
    23461 gattcgcgcg acgacggctg gcagcgtgaa gtgagcgacg atcggcgcag acaatatgac
    23521 gaccgccgcc gccagtttga agatcgccgc aggcagctgg acgatcgcca gcgccagctt
    23581 gatcaggagc gtcggcaact ggaggatgaa gagcggagaa tggaagatga gtatgggcga
    23641 tgaggttggg tatggagcag gcatcgcccc tcaccccaat ggggagaggg agaaaacgag
    23701 cgcaatattc aatattgagc acaatcggtc ccctcgcccc tctggggaga gggttagggt
    23761 gagggggcca gcgcgcacat tccctctcat cacgccaccc caatctccat cccatcaaac
    23821 cccacctcaa accctgacgg tagtgcgttt tccatcagcc acgcatcaaa ctggtggctg
    23881 atatgggtca gaatcacccg tggcgagcgg ataacctgat tcagcgcaag cacggtattt
    23941 aaatcacagt gattacgcgg tgcatccgcg cgcggcgggt gactgcaatc catcaccatt
    24001 acctgcggct gattattgcg taaaaatttc agcgtttttt ccggcaagcc agcggtgtca
    24061 gacagccacg ccacccggct gtgcgccgtt tccagtaaat agccgaaagt gagttttgag
    24121 tggttaagcg gcagcggcgt gacctgcaac ccctgcaaat caaacaccac aaacggttcc
    24181 accgtgtggc tgaaatcaag cagcccggga tgtttaaaca gatcgtcgca gccctgttca
    24241 tccggcgggc cgtagatcgg gatcggatcg cccacgcccc agcgcagcgg aaacagcccc
    24301 tggacgtgat ccatatgata atgcgtcagc aaaaactgct ggaacgatcc gggagaccag
    24361 cgatcggtga gatcgtgcag cccggcgtcg atcagggtga tcgcctcgtt aaacttcact
    24421 acgccgctgc acggttgg
//
LOCUS       DAJTVE010000057        24278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000057 DAJTVE010000000
VERSION     DAJTVE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO439_002157"
     CDS             complement(<1..200)
                     /locus_tag="OO439_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6316518.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(203..1870)
                     /locus_tag="OO439_002158"
     CDS             complement(203..1870)
                     /locus_tag="OO439_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6316519.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1867..3975)
                     /locus_tag="OO439_002159"
     CDS             complement(1867..3975)
                     /locus_tag="OO439_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6316520.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3962..4783)
                     /locus_tag="OO439_002160"
     CDS             complement(3962..4783)
                     /locus_tag="OO439_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6316521.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO439_002161"
     CDS             complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO439_002161"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6316522.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO439_002162"
     CDS             complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO439_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6316523.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO439_002163"
     CDS             complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO439_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6316524.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO439_002164"
     CDS             complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO439_002164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6316525.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO439_002165"
     CDS             complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO439_002165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6316526.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO439_002166"
     CDS             complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO439_002166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6316527.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO439_002167"
     CDS             complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO439_002167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6316528.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO439_002168"
     CDS             complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO439_002168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6316529.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO439_002169"
     CDS             complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO439_002169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6316530.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO439_002170"
     CDS             complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO439_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6316531.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO439_002171"
     CDS             complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO439_002171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6316532.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO439_002172"
     CDS             complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO439_002172"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6316533.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO439_002173"
     CDS             complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO439_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6316534.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSV"
     gene            complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO439_002174"
     CDS             complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO439_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6316535.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO439_002175"
     CDS             complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO439_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6316536.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20256..21248
                     /gene="asnA"
                     /locus_tag="OO439_002176"
     CDS             20256..21248
                     /gene="asnA"
                     /locus_tag="OO439_002176"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6316537.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO439_002177"
     CDS             complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO439_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6316538.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO439_002178"
     CDS             complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO439_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6316539.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6005 a         6312 c         5774 g         6187 t
ORIGIN      
        1 acagcgtaac gtcctgtttg atgtagatgt cttgctatca cacctaaatg cgagggcaga
       61 cctaaggtag cgaccacctt gcggttgcca tacacctcat ccaacagctg cttaggacta
      121 acaatccctt gataaacgcc tgcccgtgca atagtgctat aaatcagctc attcgagtac
      181 ggaacaggaa agtttctcat ggctaacctg cttgtttaaa taagctcgcc atatccacta
      241 ttaccccttt cccttttaac cgttcatgca tggttttttc aggttggcgt tgtgaataga
      301 tataacgtaa atccgtatca ggcaagctat cccagtcgct gggcttgact acctttatag
      361 ccgaaacctt tttactcttg gagggctttt ctagttctga cactaccgtt tcgccttcca
      421 tcaaccactg caaaacaaga ggcagtaact tttgtcttgt catcgttgga ttctggctaa
      481 acgctttttt aatagtggga attaacaggc ttgaatcgta atcctctttc agcatcagat
      541 ataaatgacg ctgatcttcg gtatctaact cttgaagggc tttttcttct ggtgtttgtt
      601 cttgtatcgc tgcgatatct agctgaagtt ggattaaccg tttatcaatc tcgggaacga
      661 ctagatcaga ataacgagca atgcgttctg ggatacccga gcgtaatgcc tctagcatgg
      721 ggtgcacagg ctttaactca tcttgataca cttgccgcaa taaaccagcg gtaatacgct
      781 cattgcctaa agctagcgca cggagctgag cgagtacaaa aagttttact acaatgtcca
      841 tcactccttg gcttagctca taccacacat cacggacctc atccgataac agcgcatctt
      901 tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa agcgatccac tcttgattgg
      961 gctttccacg ttgcgtttgt tgtataggat cccagaatat agctccaaac cctgcccctc
     1021 tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca
     1081 tcactggtac gccaataata ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc
     1141 cacccgaacg agagcggctt aaatgctgaa tttcatcaat aaccaacaac cctaaagcat
     1201 gtgcattggc tatttgcgac atcaaagcca acatggtttc tataccatga cgttttaagc
     1261 cataacgacg ctcatagttc gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc
     1321 agatttcttt tagcgaacca ttatgcgagc agtctatttt caaatacacc acctgctcta
     1381 cattgagttc acgatggtaa atcacctgag gatacgtggc tagaatacga tgaagagagg
     1441 tcgtcttccc actaccagaa caaccaatta acaataagct ttgtgccgta gatcgtgcct
     1501 cctcaaagcg aaatgtctcc aactctcccg tttgaacacg ctcataacca ttttgtaaat
     1561 gcttttgtaa atctcctgtt ttaggatttc tgcctacgta gccacctcga atcatgaccg
     1621 aaatacgctc acttagtagc aaatgcgtac ctaatggctg aaaatagtca tctggaatac
     1681 gacaaatggt atgagctctg ataacacggg acttttgcaa gtcagaggaa gtaagctgta
     1741 aagaggattt cagtgatgca gcactattca ctgactcttg taatggtggt aaggcctcga
     1801 taaaaggatt atcacgataa gcctctaccc ccgtatcacg ataaactgct tgaatccggg
     1861 tagcactcat gactcatcct tttctggtgg atcctgaaat aattcaggca cgtatgtggg
     1921 taggctgtaa tcttcttgat catccgcttc cactgcgttg aaaggaataa ctttagcctc
     1981 atcacctgaa gagcttggct tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc
     2041 ttctttttta ttagttttaa tctgcttaat gcgtgttgat ttgggctcag tagtactggg
     2101 cgttaactta ttcgctttct gaatggtttg ctgaataaac gcctcaagct ccctgcgttt
     2161 agttaactca tcctgcttcg cattggcttt attgtgtttt tcttgtgctt gtatatccca
     2221 aacctcccaa aatgagagac ctttaaactg ccgactacgt tccgtcagat tacagcgcca
     2281 aaatacacgg ctgccaactt gcggaaacaa ataaatcgta tcaaccagca ctgggtcata
     2341 agccgcttct aaatgttgag gtctagctat atcagtgctc cgctgcaacc aaccctcacg
     2401 cagaatctct gaccccgagt aatacaaacc ccacaaatta acgccaaatg aagaaataga
     2461 gacctttcgg cgaggcagta acgctactcg caactgctct tgctccacag cccttaaact
     2521 acctgtacga tgctgcatac cccattgcca tagctggaca ggaatagacg gtaaatctgt
     2581 aggaaaatca gcatctcgat cgtatttatc catcaccaga tggttatttc tgaataagat
     2641 cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga gatgcatcta acctatagtc
     2701 tgtttcacca tggcttttga tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa
     2761 ctcggcttgt agtgttctaa aagtgctttc cactatgcct ttagcatcgc cacgtctagg
     2821 tggagcactt tccactcgca cattaaaact agaaactaag gcttcgacct gatgactcat
     2881 taattcgcca cggtccgcta gcaacacatc tggcaaacct acacacggcc agtctgagct
     2941 actaatctca atatcatgct gggcacaaat ggccgttttg tcagagcaag catttacaaa
     3001 agcctgcatc gccaccacat aagacggatt ttcaaagccg atataaaagc ccgtgatcat
     3061 ccgactaaac acatcaatca caatgtaaag cgttggtctt cctatgattt tttggcgatc
     3121 atgatgatcc actaaataaa tatcagcaat cgtggcatca atctcataac gactcccagg
     3181 gcctaacgcc tgagaagtgg ctgtactact taagggtcgt acgtcttttt tatatacccc
     3241 tgctttaact ctagacttta agcgctgagc tttagggtat tctcgatcat aaaaataacg
     3301 aaactgacgt agtgttgggt aatcctcttg gggaatgcga ggaaaatact gagcaaacaa
     3361 gtccacaaat cgtctatagg caacggtggt ctttgtacct ttttgattta acaggtgctt
     3421 ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt accttggttc cttcaccctt
     3481 accatattct ctggctcggc caatctttgc tgttcctgtc gctgaacgtc tttcccctgg
     3541 tgcaccgctg tttttgtagt caggaattaa tgcattaggc gtttgaccac gctgccagta
     3601 acggcgtaac aacttataaa ctgtagcctt agtaacctta tgttcttgga ccacatgctc
     3661 aacgagttcg cttctgactt tagggtcgaa acgatcctta ctattaataa taggaagaat
     3721 ttttcgataa tcctcctcgc gcttctggaa gctgacagaa tctacagacg gctcttctaa
     3781 atcaagatgt acataagggt catcagttct aactaagcgg ccttcatcta aatactgcat
     3841 tagcaacaac tcagccctag cttgtggaac tcctttatca gcgcttattt gcatccagac
     3901 aacttggcca tcctctatag ccaaaatgcg atacggatca ttatcaaata gcacaacctc
     3961 attaatttgc cacatagcgc aactcctcca tattcactac ttggctaata cagagatctg
     4021 cacacttatt tgccctgaaa gacttataaa tattgaactt aataaaacca tttgcggtta
     4081 aggctcgtat ctcactcaat gttttgccta actccaaatc ataagcaata tcaacctgct
     4141 tacagacatt gataatgttt tcatctcctt tttcttgcag gatatgggcc aatggggata
     4201 gttgtgctaa aagctccgca gaaacttctt ctgttttcac tgaataaagc cattcaatat
     4261 tttcttttac tacgggattt atttctttat cagtaaaaat gaaccaagga atttgctttt
     4321 gctgccaata gcgacgctct agttctagtt tttctaaggt acgctcgtct tgtaaggctg
     4381 ctgcaggttt gacttgaata gcaaactgct caaaaggacc atctttgcag tccactaaaa
     4441 aatcagtaga cataacctga tctacaccac gaataacagg atgcttaata ccactatcta
     4501 ttgcaatctg cctggtatca ctaggtaata aggggaactg ctcgcgtata tctagcacgc
     4561 tgctctccca ctcaagactg agaaaaacag caagctctaa gtcagatagc aaatgatgga
     4621 ctcgtcccgt cttatgagaa taaatacggt gggaacgacc tgaagaagga acttcttgta
     4681 ctgttagcca tggaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac
     4741 gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg
     4801 ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact
     4861 ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacagcc
     4921 cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt tacgcggctg
     4981 gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca gcggaacggt
     5041 gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc
     5101 gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc cacgcgcgcg
     5161 aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg caacgacgat
     5221 aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac cagcagcgta
     5281 ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca gtgcgattgg
     5341 gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat cttctgccag
     5401 agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca gcgcctgcag
     5461 accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc
     5521 caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg ccacgccgat
     5581 ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg
     5641 aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc
     5701 cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac
     5761 ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag
     5821 cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac aggcgaggat
     5881 ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc
     5941 aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg gctgttcgta
     6001 gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt
     6061 ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg
     6121 gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcaa
     6181 cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa tcaccagcgg
     6241 actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc
     6301 gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag tcccgccttg
     6361 tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag aaacgaaggt
     6421 atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga tgccgttatg
     6481 caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag gttcaccgtg
     6541 ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt cttccgctgc
     6601 ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc
     6661 aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag
     6721 aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga
     6781 ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct
     6841 tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc
     6901 tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg acgacgccag
     6961 ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc
     7021 gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc
     7081 tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca
     7141 tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg
     7201 cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt tttcatctca
     7261 acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcaa acgagcaaac
     7321 gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata cggactgatt
     7381 tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga
     7441 tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg
     7501 tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac gcctgctaac
     7561 agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg
     7621 ttattcacac cttcaacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct
     7681 tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc attattgttg
     7741 gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg
     7801 atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact
     7861 ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc
     7921 agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc gacagagatc
     7981 agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc
     8041 tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt aaggttgtcg
     8101 tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg aacgtgcgct
     8161 gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta
     8221 tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc aaggatcact
     8281 acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc
     8341 gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc
     8401 aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac gccttttgtc
     8461 tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt
     8521 cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct atgtgaacgc
     8581 tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg cttttgtgct
     8641 tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg
     8701 ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt
     8761 gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa
     8821 ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg ccgccagaca
     8881 gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg
     8941 cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta cccgttacct
     9001 ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg tccaggtggt
     9061 aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt tttccacagc
     9121 ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt attcgccttc
     9181 catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac ccggagaacc
     9241 ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct tacgagcacg
     9301 cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg cgatgatgtc
     9361 tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc
     9421 ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt caacggccgg
     9481 gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa
     9541 ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg cctgtacgga
     9601 ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca tctcttccgc
     9661 cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg atacttccgt
     9721 accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac gaccttcgtc
     9781 acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt ttcccggcgg
     9841 ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt cggtcatttc
     9901 gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca cagagtaacc
     9961 ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt tacctacacc
    10021 cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca ggtcgataac
    10081 tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc
    10141 gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga ccggttcacc
    10201 cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga tcgggtgttc
    10261 gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg cgatggtacg
    10321 cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat taccattttg
    10381 cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga cgtcaactac
    10441 ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac gaaataacct
    10501 gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat gctggcctga
    10561 cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg
    10621 gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac cacgccctga
    10681 taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt cggatcgggt
    10741 tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc cggtaacggc
    10801 agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt gctgacaatg
    10861 taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac cggaccgatc
    10921 agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac attgccgccc
    10981 acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca ttgaacgctt
    11041 ttgtcggtcc aggtcttcat ttccgccagt agttttttga acaggttaat gttcaaacca
    11101 ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac gtcgcggtct
    11161 tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat cactttgcgc
    11221 atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg cattttggaa
    11281 gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct tgcgatctta
    11341 ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa
    11401 ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg atgcctttca
    11461 gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc atcaacggag
    11521 cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt ttcgacagtt
    11581 caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc tgcgcaacgg
    11641 acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg tggtcaagct
    11701 gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc agttcacgat
    11761 actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg gtctgtgctg
    11821 caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata ccggcgttga
    11881 acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg gtcggaacga
    11941 acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga gaaccggttt
    12001 tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca cgtgcagcac
    12061 gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca cgtcctggcg
    12121 gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac aggtcatcgt
    12181 aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg caaccggcat
    12241 acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca acgatggtgt
    12301 tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg gacgctttct
    12361 ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg atggcatcga
    12421 tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc tgaccacgac
    12481 cgattgggat catggagtca acggctttat aaccggtctg taccggctga tctacggact
    12541 gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg tgatccagcg
    12601 gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc aggccacggc
    12661 caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg gcaaggtcag
    12721 cgtacggacc cataacaacc gcacctacag agtcgcgctc aaggttcagt gcgatagcgt
    12781 aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg ccgtgaatgc
    12841 ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca ctcacaacat
    12901 tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc agttgcatgc
    12961 tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg cgtacgctgc
    13021 catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac ttatcgattt
    13081 tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa attttcgcga
    13141 gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta gcctcactca
    13201 cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga cgaccatttt
    13261 cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca caaactgcga
    13321 taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct gccatttgtt
    13381 cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct acactttggt
    13441 gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta ataaattcag
    13501 acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt cgctgttagc
    13561 agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca ggatagcaac
    13621 ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg cctgcgcctg
    13681 ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca gaatctgcga
    13741 gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt ttttcagctg
    13801 gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg cggaagcaag
    13861 gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg gccatacgta
    13921 cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt
    13981 aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct ttaaataaaa
    14041 gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca gcgatcatcg
    14101 ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga ggaatcagat
    14161 caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata ccgatcgcag
    14221 caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga tccatattca
    14281 ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa
    14341 tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat gaaggcttgc
    14401 agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca ctgtgaccac
    14461 cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat gttaccgaac
    14521 agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag gattaagttg
    14581 acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt gaagccgccg
    14641 atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag tgccatagac
    14701 agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc cagtacatgt
    14761 tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat caggaatacc
    14821 cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg gtacatgtct
    14881 ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa cttacctggc
    14941 acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa cagacccagc
    15001 accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg ggggttttgt
    15061 ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg
    15121 taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg ttgttaatta
    15181 cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc agcggcaaga
    15241 ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc agaactttga
    15301 aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta tacgcctggt
    15361 gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg cccccgctta
    15421 ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt gctatcacca
    15481 ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc gacacagaca
    15541 tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa actttcttta
    15601 aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa
    15661 acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca tacaattgcg
    15721 acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa acactagtga
    15781 taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt atttacttgg
    15841 caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact gttttaataa
    15901 atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt aacagccaat
    15961 gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact tattttctgc
    16021 ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc gttaaatgta
    16081 accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc aattttttat
    16141 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
    16201 tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
    16261 gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga
    16321 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
    16381 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc aatattctcc
    16441 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
    16501 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag tcctggtccg
    16561 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
    16621 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
    16681 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
    16741 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
    16801 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatac gctacgacgc
    16861 agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg cgtgacgcca
    16921 gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt ggcgatcact
    16981 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
    17041 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
    17101 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc
    17161 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
    17221 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
    17281 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
    17341 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
    17401 tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa catacgatac
    17461 ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata cgcctgagaa
    17521 cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accgaccagc
    17581 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
    17641 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca
    17701 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg
    17761 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg
    17821 ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
    17881 atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat cacccctgcg
    17941 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatga
    18001 gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa cgagaatacc
    18061 ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt tcgagcatca
    18121 atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg
    18181 cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt atccagaccg
    18241 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
    18301 ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat aagatcttca
    18361 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
    18421 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg
    18481 tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag accgccgagt
    18541 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
    18601 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
    18661 gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
    18721 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
    18781 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
    18841 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactacc
    18901 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
    18961 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
    19021 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
    19081 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
    19141 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
    19201 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
    19261 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
    19321 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
    19381 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
    19441 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
    19501 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
    19561 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
    19621 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
    19681 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
    19741 catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg tagtggcctg
    19801 ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc gcggaagggt
    19861 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
    19921 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
    19981 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
    20041 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
    20101 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
    20161 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg tttttgttgc
    20221 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
    20281 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
    20341 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
    20401 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
    20461 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
    20521 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
    20581 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
    20641 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
    20701 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
    20761 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
    20821 tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg agcgatggtc
    20881 atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggtc
    20941 atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat gcgtttgagc
    21001 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
    21061 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
    21121 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
    21181 atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac gtcccttctc
    21241 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
    21301 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
    21361 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
    21421 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
    21481 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
    21541 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgg gggcctgaaa gctcataacg
    21601 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
    21661 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
    21721 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
    21781 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
    21841 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
    21901 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
    21961 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
    22021 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
    22081 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
    22141 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
    22201 ttgtccgctc agcgtcatgc gttcttgaac ttcactcaac agttgttcgc gttcttcttc
    22261 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
    22321 aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact gccgggcttg
    22381 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
    22441 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
    22501 cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc
    22561 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
    22621 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
    22681 attaagcgta tccagcgtta gcattgttgt gcctggtgaa tctgtgcacc gacatcctgt
    22741 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
    22801 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
    22861 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
    22921 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
    22981 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
    23041 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
    23101 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
    23161 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
    23221 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggca
    23281 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
    23341 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
    23401 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
    23461 gcatccggta atttatccag ttgcaggcgc agcataaaaa tcagctcaaa tacatgatcg
    23521 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
    23581 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
    23641 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
    23701 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
    23761 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
    23821 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
    23881 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
    23941 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
    24001 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
    24061 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
    24121 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
    24181 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
    24241 atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAJTVE010000058        23953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000058 DAJTVE010000000
VERSION     DAJTVE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..400)
                     /locus_tag="OO439_002179"
     CDS             complement(38..400)
                     /locus_tag="OO439_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6316540.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OO439_002180"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OO439_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6316541.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OO439_002181"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OO439_002181"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6316542.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OO439_002182"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OO439_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6316543.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OO439_002183"
     CDS             complement(4827..5036)
                     /locus_tag="OO439_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316544.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OO439_002184"
     CDS             5321..5683
                     /locus_tag="OO439_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6316545.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OO439_002185"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OO439_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="OO439_002186"
     CDS             6948..11798
                     /locus_tag="OO439_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6316546.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="OO439_002187"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="OO439_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6316547.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="OO439_002188"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="OO439_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6316548.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="OO439_002189"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="OO439_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6316549.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="OO439_002190"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="OO439_002190"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6316550.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO439_002191"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO439_002191"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6316551.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO439_002192"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO439_002192"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6316552.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO439_002193"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO439_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6316553.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO439_002194"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO439_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6316554.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="OO439_002195"
     CDS             complement(19349..19780)
                     /locus_tag="OO439_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6316555.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="OO439_002196"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="OO439_002196"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6316556.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="OO439_002197"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="OO439_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6316557.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="OO439_002198"
     CDS             22866..23825
                     /locus_tag="OO439_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6316558.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6045 a         6108 c         6212 g         5588 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac ata
//
LOCUS       DAJTVE010000059        23927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000059 DAJTVE010000000
VERSION     DAJTVE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..94
                     /locus_tag="OO439_002199"
     tRNA            18..94
                     /locus_tag="OO439_002199"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Met,seq:cat)
     gene            complement(169..1422)
                     /gene="amiC"
                     /locus_tag="OO439_002200"
     CDS             complement(169..1422)
                     /gene="amiC"
                     /locus_tag="OO439_002200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6316559.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1654..2985
                     /gene="argA"
                     /locus_tag="OO439_002201"
     CDS             1654..2985
                     /gene="argA"
                     /locus_tag="OO439_002201"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6316560.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3047..4873)
                     /gene="recD"
                     /locus_tag="OO439_002202"
     CDS             complement(3047..4873)
                     /gene="recD"
                     /locus_tag="OO439_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6316561.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4873..8415)
                     /gene="recB"
                     /locus_tag="OO439_002203"
     CDS             complement(4873..8415)
                     /gene="recB"
                     /locus_tag="OO439_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6316562.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8408..11296)
                     /gene="ptrA"
                     /locus_tag="OO439_002204"
     CDS             complement(8408..11296)
                     /gene="ptrA"
                     /locus_tag="OO439_002204"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6316563.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALLGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11472..14840)
                     /gene="recC"
                     /locus_tag="OO439_002205"
     CDS             complement(11472..14840)
                     /gene="recC"
                     /locus_tag="OO439_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6316564.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14853..15176)
                     /locus_tag="OO439_002206"
     CDS             complement(14853..15176)
                     /locus_tag="OO439_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6316565.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15161..15568)
                     /locus_tag="OO439_002207"
     CDS             complement(15161..15568)
                     /locus_tag="OO439_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6316566.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15565..16128)
                     /locus_tag="OO439_002208"
     CDS             complement(15565..16128)
                     /locus_tag="OO439_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6316567.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16119..16589)
                     /locus_tag="OO439_002209"
     CDS             complement(16119..16589)
                     /locus_tag="OO439_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6316568.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16774..17568)
                     /gene="thyA"
                     /locus_tag="OO439_002210"
     CDS             complement(16774..17568)
                     /gene="thyA"
                     /locus_tag="OO439_002210"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6316569.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17575..18450)
                     /gene="lgt"
                     /locus_tag="OO439_002211"
     CDS             complement(17575..18450)
                     /gene="lgt"
                     /locus_tag="OO439_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6316570.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18601..20847)
                     /gene="ptsP"
                     /locus_tag="OO439_002212"
     CDS             complement(18601..20847)
                     /gene="ptsP"
                     /locus_tag="OO439_002212"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6316571.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20860..21390)
                     /gene="rppH"
                     /locus_tag="OO439_002213"
     CDS             complement(20860..21390)
                     /gene="rppH"
                     /locus_tag="OO439_002213"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6316572.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            22075..22764
                     /gene="mutH"
                     /locus_tag="OO439_002214"
     CDS             22075..22764
                     /gene="mutH"
                     /locus_tag="OO439_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6316573.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22833..23546
                     /locus_tag="OO439_002215"
     CDS             22833..23546
                     /locus_tag="OO439_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6316574.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23684..23902
                     /gene="ygdR"
                     /locus_tag="OO439_002216"
     CDS             23684..23902
                     /gene="ygdR"
                     /locus_tag="OO439_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6316575.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5718 a         6551 c         5984 g         5674 t
ORIGIN      
        1 taaaagcaat acggtgacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg
       61 aaggtcgtcg gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt
      121 ttttgtttct actctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct
      181 cgccagcgtt gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac
      241 ttcctgctgg aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc
      301 cgtttcgacc agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc
      361 aacttgattt ttatgcagct tgttgatttt accgagctta ttcagcactg ctttaccaaa
      421 cttaaggctg tcagcaatgg tcagcgactg caccatatcg aacatggtgt ggtcgacata
      481 gcggtcaccg cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag
      541 atattttgcc gcagtactgg ttgcaccttt ggttgagagc gcaaacaccg aggaaccgct
      601 cggctggcga ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt
      661 ctgggctttt gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta
      721 caccttcata ttgccctctt tctcgatcag agagcgcaaa cggcgagcta tttgcaatac
      781 cacatctttt tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc
      841 agggtcaagc ataatgacaa tcggacgatc gcgccctgct ttacctggtt gtggaccact
      901 ttgcgctggc ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag
      961 cagcgggtcc tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt
     1021 aaacccggcg accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa
     1081 aaccatccgt acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc
     1141 gtctgcacga atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat
     1201 atccaccacc acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt
     1261 tgattctacc gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg
     1321 gctaaccgca gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg
     1381 cagtaaacga cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag
     1441 aaatctaagc tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc
     1501 ataatctgtc atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa
     1561 aaagtcgctc tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca
     1621 ataatttcga ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg
     1681 gtcgagggat tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc
     1741 atcatgctcg gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc
     1801 gggttgttgc acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc
     1861 gacgcaaatc tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc
     1921 gacgccaaaa cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact
     1981 gctcgcctgt cgatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtc
     2041 agtggcaatt ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat
     2101 agcgggcgta tccggcggat tgatgaagac gcgatccgtc gtcaactgga cagcggtgca
     2161 atagtgctaa tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg
     2221 gaagagattg ccactcaact ggccatcaaa ctgaaagctg agaagatgat tggtttttgc
     2281 tcttcacagg gcgtcactaa tgacgacggt gatattgtct cagaactttt ccctaacgaa
     2341 gcgcaagcgc gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc
     2401 tttttgcgtg gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt
     2461 tatcaggaag atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag
     2521 attgtgatgg aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt
     2581 ctggagttga ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag
     2641 ctggagatgg aaatcgacaa attcaccatt attcagctcg ataacacgac tattgcctgc
     2701 gccgcgctct atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac
     2761 ccggattacc gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg
     2821 aagcagagcg gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag
     2881 gaacgtggat ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac
     2941 aactaccagc gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcgcc
     3001 ggatgcggca ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat ccccgcgaac
     3061 taaacaacgc cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata
     3121 tacgctcatc ggcatacagc gacagacggc gacgcgcgcg ggtcaccgcg gtgtatacca
     3181 gctctcgcgt tactatcggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact
     3241 ccgatccctg cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac
     3301 tcggttgcac agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc
     3361 cgcgatccag cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg
     3421 caatcatcac cggtcgacct tcgtaccaac gagagtgcga atgacgatga attttgcgct
     3481 tctgttgcat aaactgctca attcgctcat tcagtccagc cacgccaaat ggcccttccc
     3541 gcagggcgca caaaagttgg tactcattga acgcctgaat gattaaatcc ggctcggcac
     3601 gcgcttgcag cagatccaga taacgtccat aacccgcaag agcttcctca agcatcgcaa
     3661 tataatcttc gccgctctgt aaaagccgtt tttcaatatc agtaaaatcc tgctgaaaaa
     3721 cggttttcac tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag
     3781 aatcgctgcc gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg
     3841 cttctgtgcc ggttcctgcc ggaacgtgag ttcccgtcag gcggcttagc tgcccggcac
     3901 gctcggcggt aaagccggca ttggcataag cgcagatatc gcccagcaca gccccagcct
     3961 caaccgaggc cagttgatca cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg
     4021 cgtcgatcag tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca
     4081 catcaagatg cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc
     4141 ccagcaatcg gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca
     4201 gcggtaactg tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg
     4261 ttggtgcagc cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca
     4321 gcaacttcgc cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc
     4381 gccgcgtcag cgccactgct gccgccactt tttgccagtt aatttcatcg cttactggaa
     4441 aaagtttgtc cagggtttgc gccagtagag cttcatcaac ctcaatggca tgattcacct
     4501 cattgaagaa gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat
     4561 cgccacagag gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca
     4621 aggattcttc ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat
     4681 gcgacgcctc gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat
     4741 catgacttaa cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca
     4801 gggcaaattg cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct
     4861 tttgcaattt cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc
     4921 aatcaacccg gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac
     4981 gccacgcagg aacagataga taacgccgcc aaagtgacga tcatagtcgt aatcagcaat
     5041 gcgatggcgc agatagcgat gcagcgccag ggtataaagc tgatattgca gatcatagcg
     5101 gtgtgcctgc atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag
     5161 ccagttggat ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat
     5221 aaagcctttt aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag
     5281 cgggtcaaac tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg
     5341 caggtaaaac tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc
     5401 cgtttcattg agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca
     5461 ctgcgattca aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg
     5521 ggtaaaatcg aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg
     5581 gaactgatgc ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac
     5641 atccagccgt ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta
     5701 gctggtgaca cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc
     5761 agttaaagca tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc
     5821 aatatcatca tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc
     5881 ccctttttgc agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc
     5941 gccacgacgg cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag
     6001 cgccacgtaa agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct
     6061 ttctggcgca gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc
     6121 ctgatcctgg acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc
     6181 tttcgattta tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg
     6241 ttggctggag gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc
     6301 atgttcactt tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc
     6361 ggtaagacgc cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga
     6421 catcagcgcc cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc
     6481 gaactcttcg actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag
     6541 cgcattcagc cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg
     6601 cgtcatcacc gcctgcaaca gccaaagcat ttcctgcgct tccagagttt caaaaacact
     6661 atcgcggttc gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg
     6721 ggcggcctcc tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc
     6781 gtcgtcgccg ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat
     6841 ttgcgcagca catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc
     6901 ttccatcagc cacattttca tcgcaggctg cgtttcacct ttaaagacaa aacgtaacgc
     6961 ctgatttttc ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc
     7021 atcagtctgg ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt
     7081 ggtgtctaaa gtgtagtggg cgtgaacttc actacgcgcc ttcatataag tgaagatatc
     7141 cgcaccccgg aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc
     7201 tggctgatgg tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa
     7261 ttcatcgatc attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct
     7321 ttcgctacgc agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg
     7381 gcggcgtttt tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaccag
     7441 atcacggatc gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg
     7501 cggggtttcc cccccggctt tcgtgcgatc ttctaagaaa cgctgggaga atttttccag
     7561 cgactccggc aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttgtcgat
     7621 ccatttagcc tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag
     7681 cgcatccagt tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac
     7741 aatttgcgcg tgacgggaag ctagcgtttc atcatcgggc ggcggtgctt tgataaccgg
     7801 cgcttcgcct tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt
     7861 ttcaaagacg acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc
     7921 ggcgcaggcc tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat
     7981 gccggattca aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac
     8041 tgccgcttca tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc
     8101 gtcgacctct tccagcaggt gttcgtacaa tggattgtcg gtggtttcac gcagacaggc
     8161 gatgcgcaac tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc
     8221 ggtaaaggtg accaccagca gttcttcaac ggtcagcggg cggggaaagg cggcggaacc
     8281 gcctagtcca agtaacaggc gcaaataaag cgccgcaatc gtaaaggttt tgcctgtgcc
     8341 ggcagaggct tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc
     8401 ggcgacatca ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt
     8461 tctcccacac tttccagcct tcaggatgca catattcggc tttcccgttt tggctgccgg
     8521 aaatctgcga caggatagcc atgccttgcg gctcgactac cgcctgatgg aagaaatcgg
     8581 ccagtttttg cggcgtcagc agtttaatct gggccacgat tttatcacgc gaatcgaagc
     8641 gcatattgcc gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg
     8701 gtgcctgcag catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca
     8761 tcgctcgcaa ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg
     8821 aaggctgttt atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca
     8881 ttggaaacgc aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc
     8941 acggctgtac gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc
     9001 cagtcggtac aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga
     9061 tgacggattg ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag
     9121 cgcccaactg tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc
     9181 cgataaccat aaactctggt cgagcccctg attttaaggc gtcgcgataa gccagcacct
     9241 ctttcaacgt aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt
     9301 gcgagagcat ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat
     9361 ccatcatctg gttataccag gacttcgcct gctcaagctg atcttccgta gcggtatagc
     9421 taaagtaccc ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag
     9481 cattaaccat aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct
     9541 ggttgcttaa ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct
     9601 gattgcgggc gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg
     9661 gttcgctgga aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa
     9721 tcagctctgg atggtcatat ttcttctctg acttaatcag cgagaaatcg tcaggaatat
     9781 aggggttaag ctccggtaag gagagcgcaa tattggcagc tttttgctgc cagtcagcga
     9841 aagtttgttc gctgatttta tcgacctgat acggcgcatc gacaaaataa gccgttttat
     9901 tgtgcggctc tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca
     9961 gacgttcttt tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat
    10021 gctcaaccgg aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg
    10081 tgatcgacgg ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt
    10141 tatcaatgcc tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat
    10201 cgcgattagc caggccttta tcggttaaag acgcagagat cgctaatacg ccgctgttgc
    10261 cgttgacgat aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc
    10321 agtcggaaag tgtacctggg ctgcgattac caatcagata ggtaatcaat tcatccgttt
    10381 tactacggaa cttcgctgag ttgttatcga tgcgaaactc aacgcgcaga actttacgcg
    10441 gcattgccgg gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg
    10501 tgatttccgg ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt
    10561 ttgccaactc cggcagcggt ttattgctgt aaataaccgc cttcatcaga ttggcggaat
    10621 agtacttctc gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttatcgctta
    10681 aagtttcgag gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg
    10741 cgctgacctg tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca
    10801 ccgcattacg ctcacgttcg gcatatttct tgtcgagcaa aggttcagca atagcatcgg
    10861 ccaggcggtc taccgcacca agcaaggcgt cgttctcaac ttccagatag aaagccgtgc
    10921 gatacggcgc agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac
    10981 tgtcagcctg cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca
    11041 gcccctggta cgcctcggga tcttccagcg acccaacggg caccaccagc gccgagagcg
    11101 atttaactgc ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag
    11161 cctgatactg gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc
    11221 ccgtttctgc ctgacttaag ggtgcccaaa gggcaactaa caacaataat gctttgaacc
    11281 aggtgctgcg gggcattctc ggacctcata atcaacttaa ttttctgtcc agattcaaca
    11341 cgttaacgca aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta
    11401 tataaccatc agtccgtgac tggtgcgcat cataaagtaa gcggatagat tgcgcaattt
    11461 ttatacagca ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt
    11521 tcaacgatgg cctccattgt ctctggtgtt aattgccgcc agagcctttg ataccagata
    11581 tcatcacctt cgccacgcac catcatgtta ccttcgtaag cctgaaggaa tttcgtacgg
    11641 gctttttgca acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt
    11701 tttagccacg cgccgccact ttcaggtaac accagcaatg gcgcggacat cccttcacga
    11761 tacccctcaa tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa
    11821 cgccactcgc cgtctttgcg tagaaaaagg cgactttcac cattaccacc gctggcacag
    11881 tagacaaggt gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc
    11941 cagcgcaaca ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg
    12001 caggcgagat caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca
    12061 agctgctgca tctcctggca ctgagtttcc cagaaaattt caccaaaagc gccatacggt
    12121 aaatcccctg ccgcccggaa gcgacggaac aagcgttcgg catcatcctg ctcaaccagt
    12181 gcattcaata actgctgatt gatttgataa cggctaagcc cttccagaat aaatggctcg
    12241 gtgtcgggga tttcgctgtc ttcagtacgg aagttcacct gcaaacgcat ctggaagaac
    12301 gcccgcaccg gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag
    12361 gtaaacggca gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt
    12421 agccattcac gagcatagct ttgtcgttcg cctggctgat agttttgtgg atcaaacggc
    12481 attcgggtat ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg
    12541 agcgcttcat cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc
    12601 agtaccgacg ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag
    12661 agtttttgct gcgcggaaat taacgcttcc aggaacagat agcggtcgtc atcgcgacgg
    12721 ctacggtcgc cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga
    12781 cgtggataaa cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc
    12841 attggcatca gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct
    12901 tgatccagac gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac
    12961 tgcgcaccta aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc
    13021 gcttcggttt ccgcatccgg caggaagaag gcgctgagca tatcgcgaca aaccggcaac
    13081 cactcttcta gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc
    13141 agtgaagcca gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga
    13201 accgattgcc actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg
    13261 ccaaatcgcc aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca
    13321 tctatccccc aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct
    13381 tcggtgatgt caaaccgcgc cgccagcacc ggcacatcca gtaatgccag cacgtcttcc
    13441 gacacaaagc ggctgtctgg caacgataac aggctgataa acgcttccag taccggatgc
    13501 gactgccgcg cccgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta
    13561 ccaaacacag cccgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg
    13621 tcgcgcggag taagtgtcgg gtcttcttcc agcatcgcca gcaggcgatc gtgcaaaact
    13681 tcaacttcac gctgcgggct atggcaaacg tggaaggtga tactgctatc cagtggatca
    13741 agcgggcgtt tgttatcgct acgggaaaac tcttcgatgt tcacaccagc aacggcacgg
    13801 ttttccagtt ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtgacatcg
    13861 acaaaggcgt ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc
    13921 ccaagcttac cccatgaagc cagcagcggg ttgccgacat cctgttcacc atcgctgtta
    13981 aagagctgcc cggcattttc gctatcacga aatagcggca attcgcgatc ttcaaaactg
    14041 tgtcgacgct gacgggtcag cagtttcgcc agataagcag gatctttaat atcgccccag
    14101 taataacggc aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgt
    14161 agcgcctgga gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt
    14221 aaccccggcg ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattg
    14281 gcgcggtgcc agcgcggttg cccgagttga tgggtatatt ccaccagcgc cttccacaac
    14341 ggggcttgcc aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt
    14401 gccagccagt ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa
    14461 agctggaaca gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag
    14521 tcttcgcgct ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg
    14581 ttaaaggcgc tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag
    14641 ctcgctggca gcggaaaatc aatgtttgcc gcaatactaa acttttgcga cagggtcatt
    14701 tgcagccact gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg
    14761 tccagccgtt cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg
    14821 gaatggtaga cccttaacat agcggctcct gactactgac gattcgggca atgcaggcgg
    14881 gtcatctcgc cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc
    14941 gcctgcgatg tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt
    15001 tgcgtttgct gccaaccatg ccgccagagc tgttggtact ggtttctgct ggcgagactg
    15061 ttcattaatg ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc
    15121 gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataacg
    15181 ccctctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atgtttccat
    15241 caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaacc aataaggctt
    15301 cattatctgc cagtaaacgc aaacaaaccc gggcatcggt tccagcgtac tgcgagcact
    15361 gaactgcggg ctgcgtctgc caggagtgca ttttccccca cgccagcgcc gactgaacga
    15421 tggcctggcg gcgcaatgac tgactttcta tgctcacgcg agaggcaaaa ctgcgatcct
    15481 gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca
    15541 gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc
    15601 caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga
    15661 gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt
    15721 agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac
    15781 atgctccttc agacgaaatc caatctggtc agactctttt accggttcgc gatcccagat
    15841 accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag
    15901 cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt
    15961 aaatacccgc agccagattt catcttccag cgccagctta cgggtgctcg ttaaactttc
    16021 acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat
    16081 agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct
    16141 ccttgctgac aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt
    16201 gagtttttga agcgaatatg cccggcccat gcggtattgc gcaggccaaa gaaagcaagc
    16261 gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa
    16321 ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc
    16381 ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc
    16441 aaatagtccc gcgcctggct ggcggtttgc catagccgtt gcgactgctg ccagtattgc
    16501 cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca
    16561 atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga
    16621 cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg
    16681 ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa aaccgacgca
    16741 cactggcgtc ggctctggca ggatgtttcg taattagata gccaccggcg ctttaatgcc
    16801 cggatgtgga tcgtagcctt caatctcaaa gtcttcgaaa cggtagtcga agatggattc
    16861 gggtttacgt ttgataatca acttcggcag cggacgcggt tcgcggctta attgcagatg
    16921 agtttgatcc atatgattgc tgtacaggtg cgtatcgcca ccggtccaga caaaatcacc
    16981 cacttccaga tcgcactgct gcgccatcat atgcaccagt aacgcgtagc tggcaatgtt
    17041 gaacggcagg ccgaggaaga cgtcacagga gcgctgataa agctggcaag agagtttgcc
    17101 gtctgccaca tagaactgga agaatgcatg gcacggtgcc agcgccattt tatccagttc
    17161 gcctacgttc cacgctgaaa caataatgcg gcgggaatcc gggtcgtttt tcagctggtt
    17221 cagtaccgta gtgatctggt caatatgacg accatctggc gttggccagg cgcgccactg
    17281 tttaccatac actggtccga ggtcgccgtt ttcatcggcc cattcgtccc agatggtgac
    17341 attgttttcg tgtagataag caatgttagt gtcgccctgc agaaaccaca gcagttcgtg
    17401 gatgatggaa cgcaggtggc aacgtttagt tgtcaccagc gggaatccat cctgcaggtt
    17461 aaaacgcatc tgatgaccaa aaatggaaag cgttccggtt ccggtacggt cgtttttctg
    17521 tgtgccttcg tcgagcactt tttgcatcag ttctaaatac tgtttcatgg ttcctcagga
    17581 aacgtgttgc tgtgggctgc gacgatatgc ccagaccatc atgatcacac ccgcgacaat
    17641 catcgggatg gaaagaattt gccccatgct gatgtactgc acccaggcac cggtaaactg
    17701 cgcgtcgggc tggcggaaaa actcaacaat gatgcgaaac gcgccgtagc caatcaggaa
    17761 caagccagag acagctccca ttgggcgcgg tttacgaata tacaggttga ggataataaa
    17821 cagcaccaca ccttccagca gcagctcgta aagctgtgat gggtggcgcg gcagcacacc
    17881 gtaagtgtcg aaaatggatt gccactgcgg gttggtttgc agcagcaaaa tatcttctgt
    17941 acgggagcct gggaacagca tggcaaacgg gaagttcggg tcaacgcggc cccacaattc
    18001 accgttaata aagttgccca gacgcccggc accaagacca aacggaatga gtggtgcgat
    18061 aaaatcagaa acctggaaga aggaacgttt agtacggcgg gcaaagataa tcatcaccac
    18121 gataacgcca atcaggccgc cgtggaaaga catgccgccg tcccagacac ggaacagata
    18181 cagcggatcg gccataaact gcgggaaatt gtagaacaga acataaccaa tacgtccccc
    18241 gaggaagacg ccgaggaagc ccgcatagag taagttttca acttcatttt tggtccagcc
    18301 gctgcccgga cgattcgccc gtcgtgttgc cagccacatt gcaaaaatga aacccaccag
    18361 atacatcagg ccgtaccagt gaagcgccac gggtcctatt gagaaaatga ccggatcaaa
    18421 ctccggaaaa tgcagatagc tactggtcat ctgtcaccac aagttcttgt tatttcgctg
    18481 aaagagaaca gcgattgaaa tgcgcgccgc aggtttcagg cgctccaaag gtgcgaataa
    18541 tagcacaagg ggacctggct ggttgccgga taccgttaaa agatatgtat atgatccgcg
    18601 ctataaccct ccgcgaatca gcccgcccat gccgcgacgc tccataaagg ctgcaacctg
    18661 atggcgaact tcggtcgcca gttgcgcttc cagactacgc tgcgcaagat tttctgcttc
    18721 ggcataatca atgcgccgca gcaggtattt tacccgcgct acagaacgtc cgttcataga
    18781 cagatggcga tacccaagcc caatgaggat tgccacgcac atgggatcgc ccgccatttc
    18841 accgcacaaa cggagatcga ttccatgtat ttccgcttcc cgggcgatca tcgccagagc
    18901 tcgtaacatt gcaggatgaa gactgtcata aatgttcgcc acccgggtat tgttgcgatc
    18961 aacggccaga atgtattgag tcagatcgtt ggtgccaaca gagatgaaat cgacccgctt
    19021 tgccagatgc ggcagcataa ataccattga cggcacttcc agcatgatgc cgatacgtgg
    19081 tttgggaatt tcgtaaccga tcatctcctc gacttcacgt ccggcacgtt caatcaggcg
    19141 gcgtgcttca tcaacttcat cgaggcttgt gaccatcggc aacagaatat tcaggttgcc
    19201 cgtagcggca ttagcacgca gcatcgcccg cacctggatc aagaagatct ccggctgatc
    19261 gagcgtaatg cgaatcccac gccaacccag gcatggattc tcttcgctga tcggcatgta
    19321 aggcagctgc ttatctgctc cgacatccag cgtacgcaag gtgacgggtt tatcattgaa
    19381 catttgcagc atcccctgat actgcgccac ctgttcttct tccgacggga aaccactttg
    19441 cagcatgaat gggatttcag tgcgataaag accgatgcca tcaatacggc tgcccagttt
    19501 ttcttcatgt tccgggctta aaccagcatt gagcatgact tttatacgct caccgctttt
    19561 taactgcgcg ggtaaattga cgtcatcttc cgccagacgg ctaagctcaa tctcttcact
    19621 aattagccgc tgatattctt gcagcagtac cggctccgga tcgaccagca attcaccgcg
    19681 atagccatca acgatcagcg tcctacgatg cagcaccgaa ggctgaatat ccgcgcccat
    19741 cacggtaggg atccccagcg cacgtaccat gatcgcagca tgggagttgg ctgcgccatc
    19801 tcgcacgaca acaccgacta agcgatcctg gggcagctca gcaagagttg tcgctgacag
    19861 ttcatctgcc accagaatga aacgttccgg ccaggcgttc ggcccttgat tagcgtcatc
    19921 aagatgaaac agcaatcgct gacccagcgc acgtaaatcg ccagcccgct ctttgagata
    19981 gttatcgctt agcgcggcaa actgttcggc aaatttttca atgaccgttt ttaccgccca
    20041 ctctgccacc gagcctttat caacctcggc aaacaattcg cgacgcagcc gggtgtccga
    20101 aagtaggtgc gagtaaagat cgaaaatagc cgccgtttct ttttgtgctc cggcggcaaa
    20161 gcgtttgctg tagcggcgaa actcgtttgc cgcttcttcc agcgccccgg tcagtcgttc
    20221 gcgttccaga gccggatcca gcgttgatgc ctgatacacc tgttccatta aaggtaacgt
    20281 ggcatcctgc cagccttcgg caatcgccac accaggtgct gccggtaatg cgcggattcg
    20341 cgtctggcga tattgcccaa acaaggcagt caactgcgac tgagaaagaa tagctgccat
    20401 ctgggtggca agcgtcacca ggaaggattc ttcactttcg tcatactggc gcaactctcg
    20461 ttgctgtacc accagtacac caagcaactg gcgacgttga ataattggta cgcctaaaaa
    20521 cgcgcggaaa cgttcttctt ttacggaggg gatgtatttg aagctggggt gcttttgcgc
    20581 atctgcaagg tttatcggtt ccgccagcct gccaaccagg ccgacgatcc cttcatcaaa
    20641 cgcgagcgtt acagtgcgac cgcgtggttt tttcagcccc cgggtcgcca tcaggtagta
    20701 acaacgtcga tcatgatcgg ccaggtagac cgaacagacc tcggtatcca tcgcaagaca
    20761 gatgtcggta accagaatat ttaacgcctc attcaggcgt ggtgcgctgg ctaccttttc
    20821 gactatttcg cgcaggcgag tgagcataat tggcgtgact taacctcttt tacgtcgata
    20881 agcagatgtg ttttgtggtt ttggcgtatt ttcctgcagt gacatcacca cactcgcgaa
    20941 ctctttcatt accctacggt agacatcacg tttaaatgac accacctgtc tgaccggata
    21001 ccagtaactt acccatcgcc agccgtcaaa ctctggtgta ctgctggttt gcatattgat
    21061 ttctgcatcg ccgctcacca gctgcaagag aaaccatttt tgtttttggc cgatacaaac
    21121 cggcttcgtg tcccaacgca ccaaacgttt cggtaatttg tagcgcaacc agttacgcgt
    21181 tgaagcaagg attcgaacgt ctttgcggct taatcctact tcttcaaaca attcacggta
    21241 catcgcctgc tctgcggatt ctccggggtt gattccgcct tgcggaaatt gccaggagtg
    21301 ctgaccaaat cgccgggccc acattacctg cccctggcga ttacaaatca cgatacctac
    21361 gtttgggcgg tagccatcgt catcaatcac cggactacct caaaataaag ctttatatac
    21421 gaatgattgt ttcatactcc aagaagacgg taaaccactc tctgcagggc attacacact
    21481 aataacaatt gaataactca cagttatgtg cagagttata aacagaggaa ggggtggata
    21541 gccgttttta ttcacctttt ctgtggatag agttgtgaag aactacggaa ttactacggg
    21601 aaaacccgga gcattctgaa taagcccgat atagacattt taaaatattc attattagtc
    21661 aatgcattac atgtaatttg agtaatgaaa aagagtaatt cgtgacccag gtcacacctc
    21721 tcatttacgg gttggcgaaa gatcaaccaa tgccgtattt atccacagaa tgtgccacta
    21781 agttaagcac tgaaccacta aaaactggag tttcgtcgca cgtcaaggct gtaaatggaa
    21841 acagtagtgg aggtttttca cagttatccc agctttctgt ggataacatg gtgtaagatc
    21901 ctgtttattt tcagtgacca gatttggaaa acctattgca gtgttgcgca actcgattac
    21961 cggcagccta aaaagcaata taaatcagag aattgaacaa cacatgtgga aaaagttaca
    22021 ctgcgaatat tcggcacata attgctgttt gttttttaac caaggtatca tgacatgtcc
    22081 caacctcgcc cactcctctc tcctcccgaa actgaagaac agttgttagc gcaagcacag
    22141 caactttctg gttatacatt gggagaactg gcggcacttg ccgggctggt tacgccagag
    22201 aatttaaaac gcgataaggg ctggattggc gtgttactgg agatctggct aggtgccagc
    22261 gcagggagta aacctgagca agattttgct gctctgggcg tggaacttaa aactatccct
    22321 gtggatagtc ttggtcgtcc gctggaaaca acattcgttt gtgttgcccc gttaacgggt
    22381 aatagcgggg tgacctggga aaccagccat gtgcgccaca agctcaaacg cgtactgtgg
    22441 ataccggttg aaggtgagcg cagcatcccg ctggcgcagc gtcgcgtagg atcaccgttg
    22501 ctgtggagcc cgaatgaaga ggaagaccga cagctacgcg aagactggga agaattaatg
    22561 gatatgattg ttctcggtca ggttgagcgg atcaccgctc gtcacgggga gtatttacag
    22621 atacgaccga aagcagcgaa tgcgaaagcg cttaccgaag ccattggtgc ccggggcgaa
    22681 cggattctga cgctgccacg cggcttttat ttgaagaaga atttcaccag tgcactactg
    22741 gcccgtcatt ttctgatcca gtagccatcg ctttgacctg ccgctttccg ggcatataat
    22801 taccgcttca tttttttggc agggcttttt agatgttatt tgcatggata accgatccta
    22861 acgcctggct tgcgctcggt acgctgacgc tgctggagat cgttcttggg atcgacaata
    22921 ttattttcct ttctctggtg gtggcaaagc tccccacagc acaacgtgct catgcgcgcc
    22981 gtctggggtt ggcgggagcc atggttatgc gtctggcgct gctggcatca atcgcctggg
    23041 ttacgcgcct gacaaatccg ctttttacaa tattcagtca ggaaatttcc gcccgtgatt
    23101 tgattctgct tctgggtggc ttgttcctta tctggaaagc cagcaaggaa atccacgaat
    23161 ccattgaagg tgaagaagaa gggctgaaaa cacgcgtttc atcattcctc ggcgctatcg
    23221 tgcagattat gctgctggat attatcttca gcctcgactc ggtgattacc gctgtgggtc
    23281 tgtcagatca cctgtttatt atgatggccg ccgtggtgat tgccgtaggc gtgatgatgt
    23341 tcgccgcgcg ctcgattggt gattttgtcg aacgccatcc ttcggtaaaa atgctggcgc
    23401 tctctttcct gattctggtc ggctttaccc tgattctgga aagtttcgac atccacgtac
    23461 cgaaaggtta catctacttc gcgatgttct tctctattgc ggttgaaagc ctcaacctga
    23521 ttcgcaacaa aaagaacccg ctctgatact ccgtacgctc tcctgcggga gggcgtactc
    23581 actcctgtta ccctcacagt ttaagatttt cctgctttca agatatataa cgtcggttta
    23641 taaacagact attatcatag gtgggccaaa tgaggataac cggatgaaaa aatgggcagt
    23701 aataatttct gcagtcggac tggcgtttgc tgtttccggg tgttccagtg attacgtcat
    23761 ggcgaccaaa gatggccgta tgattttgac cgatggaaaa cctgaaattg atgatgatac
    23821 cgggctggtg agttatcacg atcagcaagg taacgcgatg caaattaacc gtgatgatgt
    23881 ttcgcaaatt attgaacgtt aacaaataag gtcagcatcc ggctgta
//
LOCUS       DAJTVE010000060        23421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000060 DAJTVE010000000
VERSION     DAJTVE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO439_002217"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO439_002217"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6316576.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OO439_002218"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OO439_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6316577.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO439_002219"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO439_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6316578.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO439_002220"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO439_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6316579.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO439_002221"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO439_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6316580.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OO439_002222"
     CDS             5281..5739
                     /locus_tag="OO439_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6316581.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OO439_002223"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OO439_002223"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6316582.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OO439_002224"
     tRNA            6791..6875
                     /locus_tag="OO439_002224"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="OO439_002225"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="OO439_002225"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO439_002226"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO439_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6316583.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO439_002227"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO439_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6316584.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO439_002228"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO439_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6316585.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="OO439_002229"
     CDS             complement(10157..13900)
                     /locus_tag="OO439_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6316586.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO439_002230"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO439_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6316587.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="OO439_002231"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="OO439_002231"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6316588.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="OO439_002232"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="OO439_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6316589.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO439_002233"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO439_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6316590.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="OO439_002234"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="OO439_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6316591.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO439_002235"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO439_002235"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6316592.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO439_002236"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO439_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6316593.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="OO439_002237"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="OO439_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6316594.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23295..23402
                     /locus_tag="OO439_002238"
     CDS             23295..23402
                     /locus_tag="OO439_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6316595.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5871 a         6123 c         5645 g         5782 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgccttg ttgtcattac
    23281 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac gacctggcag
    23341 caccgatcct ggcggggatt attaccgcag cgattgtcag ctggtggcgt aaccggaagt
    23401 aaggtgaaaa cgggcgtcag g
//
LOCUS       DAJTVE010000061        23237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000061 DAJTVE010000000
VERSION     DAJTVE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO439_002239"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO439_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6316596.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="OO439_002240"
     CDS             509..1138
                     /locus_tag="OO439_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6316597.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="OO439_002241"
     CDS             1165..2826
                     /locus_tag="OO439_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6316598.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="OO439_002242"
     CDS             3065..3124
                     /locus_tag="OO439_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6316599.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="OO439_002243"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="OO439_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6316600.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO439_002244"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO439_002244"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6316601.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO439_002245"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO439_002245"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6316602.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO439_002246"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO439_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6316603.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="OO439_002247"
     CDS             9507..10127
                     /locus_tag="OO439_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6316604.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="OO439_002248"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="OO439_002248"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6316605.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO439_002249"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO439_002249"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6316606.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO439_002250"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO439_002250"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6316607.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="OO439_002251"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="OO439_002251"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6316608.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO439_002252"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO439_002252"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="OO439_002253"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="OO439_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6316609.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="OO439_002254"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="OO439_002254"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6316610.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="OO439_002255"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="OO439_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6316611.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO439_002256"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO439_002256"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6316612.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="OO439_002257"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="OO439_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6316613.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="OO439_002258"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="OO439_002258"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6316614.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="OO439_002259"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="OO439_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6316615.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5653 a         6332 c         5906 g         5346 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcaccctgtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgcctgt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttacgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAJTVE010000062        23147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000062 DAJTVE010000000
VERSION     DAJTVE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            221..1654
                     /gene="cscA"
                     /locus_tag="OO439_002260"
     CDS             221..1654
                     /gene="cscA"
                     /locus_tag="OO439_002260"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6316616.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1662..2720)
                     /locus_tag="OO439_002261"
     CDS             complement(1662..2720)
                     /locus_tag="OO439_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6316617.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2888..3106
                     /locus_tag="OO439_002262"
                     /pseudo
     CDS             2888..3106
                     /locus_tag="OO439_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3124..4452
                     /gene="dsdA"
                     /locus_tag="OO439_002263"
     CDS             3124..4452
                     /gene="dsdA"
                     /locus_tag="OO439_002263"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6316618.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4560..6098)
                     /gene="emrY"
                     /locus_tag="OO439_002264"
     CDS             complement(4560..6098)
                     /gene="emrY"
                     /locus_tag="OO439_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6316619.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6098..7261)
                     /gene="emrK"
                     /locus_tag="OO439_002265"
     CDS             complement(6098..7261)
                     /gene="emrK"
                     /locus_tag="OO439_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6316620.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7677..8291
                     /gene="evgA"
                     /locus_tag="OO439_002266"
     CDS             7677..8291
                     /gene="evgA"
                     /locus_tag="OO439_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6316621.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8296..11889
                     /gene="evgS"
                     /locus_tag="OO439_002267"
     CDS             8296..11889
                     /gene="evgS"
                     /locus_tag="OO439_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6316622.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11945..13090)
                     /gene="yfdE"
                     /locus_tag="OO439_002268"
     CDS             complement(11945..13090)
                     /gene="yfdE"
                     /locus_tag="OO439_002268"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6316623.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13164..14108)
                     /gene="yfdV"
                     /locus_tag="OO439_002269"
     CDS             complement(13164..14108)
                     /gene="yfdV"
                     /locus_tag="OO439_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6316624.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14178..15872)
                     /gene="oxc"
                     /locus_tag="OO439_002270"
     CDS             complement(14178..15872)
                     /gene="oxc"
                     /locus_tag="OO439_002270"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6316625.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15926..17176)
                     /gene="frc"
                     /locus_tag="OO439_002271"
     CDS             complement(15926..17176)
                     /gene="frc"
                     /locus_tag="OO439_002271"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6316626.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17689..18312)
                     /locus_tag="OO439_002272"
     CDS             complement(17689..18312)
                     /locus_tag="OO439_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6316627.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18609..18884
                     /gene="ypdI"
                     /locus_tag="OO439_002273"
     CDS             18609..18884
                     /gene="ypdI"
                     /locus_tag="OO439_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6316628.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18961..19203)
                     /locus_tag="OO439_002274"
     CDS             complement(18961..19203)
                     /locus_tag="OO439_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6316629.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19556..20476
                     /gene="lpxP"
                     /locus_tag="OO439_002275"
     CDS             19556..20476
                     /gene="lpxP"
                     /locus_tag="OO439_002275"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6316630.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20832..20903
                     /gene="ypdK"
                     /locus_tag="OO439_002276"
     CDS             20832..20903
                     /gene="ypdK"
                     /locus_tag="OO439_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6316631.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20968..22206)
                     /gene="alaC"
                     /locus_tag="OO439_002277"
     CDS             complement(20968..22206)
                     /gene="alaC"
                     /locus_tag="OO439_002277"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6316632.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22583..>23147
                     /gene="ypdA"
                     /locus_tag="OO439_002278"
     CDS             22583..>23147
                     /gene="ypdA"
                     /locus_tag="OO439_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6316633.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6484 a         5418 c         5042 g         6203 t
ORIGIN      
        1 gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc tttctcgcaa
       61 aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata gttaacgtta
      121 acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat aaaagttaac
      181 gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat ctcgattgca
      241 tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt tctatcccca
      301 ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga tctggtttaa
      361 cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg gaccaatgca
      421 ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta ttgcgctagc
      481 gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg atgacaatgg
      541 tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta atgacgatgc
      601 aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg agaaacaggg
      661 tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag tgtggcgtga
      721 agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg ggcagatcct
      781 gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg cccacgctga
      841 tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg atcagcatta
      901 tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa atcgctttca
      961 aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat ccgggcattt
     1021 tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag cgaaggatgg
     1081 tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct caaaacgtga
     1141 aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg gcaaacttct
     1201 acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg tctctccccg
     1261 cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga ttcagttgca
     1321 gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca ctggaatgcg
     1381 gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc cacacgagaa
     1441 tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc taaggatatt
     1501 tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga tgagtagtcg
     1561 aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg gagtggctgt
     1621 gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga acaacggatc
     1681 aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat aatatgcaat
     1741 gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg cggtaaaaac
     1801 agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg cgggatacgt
     1861 acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt accacatatc
     1921 agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact tgcgagatcg
     1981 gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag atctcgtcca
     2041 gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa cgcactttcc
     2101 ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg tgttgattca
     2161 tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc cgccacacag
     2221 ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg tcgcagcccc
     2281 attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc cgcgcgggca
     2341 gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc gctggcggtt
     2401 tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc ctgcgccagc
     2461 acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga aagatcagga
     2521 acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg acgcacagat
     2581 tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag gcgtgcgaca
     2641 tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta gcagaaacag
     2701 acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata aaaaataaca
     2761 ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag taaccatata
     2821 ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa tataaatatg
     2881 gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga ttgctatcgg
     2941 ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag tgaagcaata
     3001 ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat ttatcgcttc
     3061 agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc aaagagacgt
     3121 actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt aaaggatctg
     3181 gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc tgaaggttta
     3241 ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt atcccgtttt
     3301 gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat tattgaatca
     3361 gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca gcaaccgatc
     3421 agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc cataaaagca
     3481 cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga agcggggttg
     3541 ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca gttctttagc
     3601 caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg cattatgagc
     3661 gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc atggaaaaaa
     3721 gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta tggtgttgcc
     3781 gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat tgatgacgaa
     3841 aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa agcgcaattt
     3901 gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct gccgtgtggt
     3961 gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg cgatcatgtt
     4021 cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt ccatacagga
     4081 ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc agcggatggc
     4141 cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct gctggatggc
     4201 ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc gcaggaagaa
     4261 ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg cgtatgtgca
     4321 tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa taccactcat
     4381 ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca atatctggca
     4441 aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt gagcgatgct
     4501 gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc cttttatcat
     4561 cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac cgtaagtaag
     4621 ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag tgactgctgg
     4681 gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata tttatccata
     4741 atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt caactgacta
     4801 tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc aactgatcct
     4861 gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt atctggcaag
     4921 cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa tccctgaaaa
     4981 aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac agaacgccag
     5041 taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat gtctattttg
     5101 ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat gggcgcataa
     5161 gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg cggcataagg
     5221 acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc aatggtgaag
     5281 ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga ctcccaaatg
     5341 actaaagaga tcagagaaat aactgatact actgttaata ttattattgt actcgagttg
     5401 aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac accgagcact
     5461 aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc tcttccttta
     5521 agtaaggtta agcataatgt caggacgata atccccatag ggacattgat taaaaatatc
     5581 caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca tatcggagcg
     5641 ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg cggataattc
     5701 cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc ctgaacgact
     5761 ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga agacagcgaa
     5821 aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc caacctgcca
     5881 gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca ggtgccttcg
     5941 tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga aatagtggaa
     6001 tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca taacgtccca
     6061 ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca ttatgcgaaa
     6121 taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc ttactggtat
     6181 aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg tcttcgtcct
     6241 tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc atgagttctt
     6301 ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag ttccctgtcg
     6361 cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc cctgtcaccc
     6421 gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact gattgaccaa
     6481 tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt tgacgtgccg
     6541 gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga acacttcgct
     6601 gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc aaccaggctt
     6661 ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt gtgttcatta
     6721 ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct ttgctactta
     6781 ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc ttcgctaaag
     6841 gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct gaagcgactt
     6901 cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca atatttgcca
     6961 gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt gaaactaaaa
     7021 tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca ctacctgaga
     7081 cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta ctaatcatgt
     7141 cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat aaaactatca
     7201 ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta atctgttcca
     7261 ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga gtattcattc
     7321 ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt
     7381 aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta
     7441 cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa tcatcggtat
     7501 aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg tatacttgtc
     7561 gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct
     7621 tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga
     7681 acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca
     7741 aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt cagcgggtgg
     7801 aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc
     7861 tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc gtctccgcta
     7921 aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta
     7981 aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct
     8041 atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg
     8101 actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata
     8161 atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc
     8221 tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca caacgtaaca
     8281 aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg tggtctttgg
     8341 tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac
     8401 cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa
     8461 aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca taccgattcg
     8521 cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag aacgttaaat
     8581 atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc gctggaagaa
     8641 ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa tgatgacatt
     8701 gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct tcacgattca
     8761 atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccct
     8821 gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat
     8881 caggcattag catccgtctc agccggacag aatgattact ttattggtag taacatcatt
     8941 accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac
     9001 tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct taatgaagta
     9061 ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc acaaaattgg
     9121 cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga acatgaaaaa
     9181 caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc ccctccctat
     9241 tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct taatattatt
     9301 actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgctgga
     9361 acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag tgaagataga
     9421 gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa
     9481 aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat
     9541 tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat aaaagtcgat
     9601 aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct ggtcgcgaca
     9661 cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta tcattttctt
     9721 attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt
     9781 aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct gcgcctgacc
     9841 gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa
     9901 ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattt
     9961 tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata
    10021 tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt aaactggcaa
    10081 ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga ttactacaaa
    10141 aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt ttctaatgcg
    10201 catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt ttttgaaatt
    10261 gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa tctgccagcc
    10321 agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg tgatttaatt
    10381 catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagtcagttt
    10441 ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg cttcctggaa
    10501 cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc acttgcctac
    10561 tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa
    10621 tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact
    10681 tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg cagcagtaca
    10741 tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat
    10801 ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg
    10861 gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc tggaagtgga
    10921 ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag tgcaggtcgc
    10981 cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa aaatatgcag
    11041 ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg
    11101 gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc catcacacta
    11161 cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa
    11221 cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta
    11281 cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg
    11341 gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc catctggggg
    11401 cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg catgaactta
    11461 tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag
    11521 gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa aaataatacg
    11581 gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga aacgcataaa
    11641 gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt ccatcagtgt
    11701 attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc
    11761 catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgctg
    11821 aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa
    11881 aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc agcacatctt
    11941 acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc
    12001 ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta
    12061 ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta atggcctcag
    12121 ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct aaccaaactt
    12181 cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc tggttttgta
    12241 cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc tccagtgcct
    12301 ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag
    12361 tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt tgtggtgact
    12421 tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg gcatcaaaca
    12481 tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt gcactcacta
    12541 ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca acacgcactg
    12601 gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga atgatggtat
    12661 cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata
    12721 tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt tccattgtac
    12781 ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata aatatacttt
    12841 tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta ataaaactgt
    12901 aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacctg
    12961 gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc
    13021 cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat
    13081 tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta
    13141 tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga
    13201 gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt acgggtgtag
    13261 acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt
    13321 accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag aagcagcgcc
    13381 agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact gaactcgaat
    13441 ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca
    13501 atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag caccaggatc
    13561 gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa tgcgctcaga
    13621 ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga
    13681 atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa
    13741 tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacccgca
    13801 attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac
    13861 caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag acgagtgtcc
    13921 gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc aggaagagcg
    13981 tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt ttctcgtctg
    14041 ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat
    14101 gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga
    14161 ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg
    14221 ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg tttgcgcgac
    14281 tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg
    14341 ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc cgttggtgat
    14401 ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa
    14461 ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa
    14521 ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac gctagcaccg
    14581 atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt
    14641 ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact
    14701 aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa
    14761 tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg
    14821 tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagttct
    14881 gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc aatggggcgg
    14941 ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat
    15001 ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat gacaacgtca
    15061 gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg
    15121 atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat aaattcacga
    15181 agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca
    15241 gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc
    15301 gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc
    15361 aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac agagacccgg
    15421 atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg
    15481 gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg
    15541 acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg
    15601 ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag gcagatcccc
    15661 ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga
    15721 aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt cacaggaata
    15781 cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc
    15841 ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat
    15901 tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt
    15961 catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct aatagcggcg
    16021 cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg gtcagatatt
    16081 ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa gagggatcaa
    16141 gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaatagg
    16201 ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga
    16261 aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc cattctggtt
    16321 tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg
    16381 cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac
    16441 ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac tgcgggtatt
    16501 cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg cggcaaaggt
    16561 tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat
    16621 gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg tcacccaacg
    16681 ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc gcgccacccg
    16741 ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc
    16801 ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc
    16861 ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca
    16921 gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt
    16981 tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc agttggtgac
    17041 gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca aaccaggcca
    17101 gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt
    17161 gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa tttagcgttt
    17221 atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc ggcactcgat
    17281 atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc aattcctttc
    17341 tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc gtggtaatca
    17401 gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatttac
    17461 aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata ataaatgatt
    17521 tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt gtttatagaa
    17581 ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt
    17641 cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt agttcacaaa
    17701 cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc
    17761 gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt
    17821 aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa gctcttccat
    17881 tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg cggcttcttt
    17941 ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga caatatattg
    18001 gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt cggtactgtc
    18061 atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg gatcgccatg
    18121 aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat
    18181 acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg ctaccggtgc
    18241 attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg tagccataat
    18301 taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc atcatattgg
    18361 gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta tcttacaatt
    18421 gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc ttcctgtcgc
    18481 agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg taaatattga
    18541 ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca ttttcaggat
    18601 aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc tctattatgg
    18661 catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc tataaagaga
    18721 ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca tcacaggcga
    18781 ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa atcacgccta
    18841 cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca
    18901 ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca
    18961 ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa
    19021 cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga agtaataaat
    19081 gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca cgttcagtac
    19141 ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat
    19201 catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga
    19261 caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg ttaaatgcaa
    19321 cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag ctgaaagcag
    19381 tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg tcctgactag
    19441 tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc
    19501 gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt
    19561 tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca catggtttgg
    19621 gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac
    19681 gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa
    19741 cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga ttgccgaaaa
    19801 ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct ggcccgacag
    19861 tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg cacaaatgca
    19921 aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt
    19981 gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga
    20041 atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct
    20101 gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga
    20161 ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac
    20221 caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg taacgatggt
    20281 aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg aaggctaccc
    20341 gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg
    20401 cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc
    20461 gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg ccataataac
    20521 ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat ctaattatta
    20581 ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa agtaaataag
    20641 aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt gagcagttta
    20701 gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt cgctttgccc
    20761 ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt aattacgcgg
    20821 agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg
    20881 gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc
    20941 tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat gtttgctgct
    21001 ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg cctgacgaat
    21061 acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc
    21121 aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa attccagcga
    21181 tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag ccttcggcat
    21241 ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc ggcgtttgta
    21301 ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa tcgccgccac
    21361 ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca gggcactgac
    21421 cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt agcttttcga
    21481 cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa ttgacggcgc
    21541 tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca gcacatcgta
    21601 gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt gcgcggttgg
    21661 gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac gaatggcgcg
    21721 ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc
    21781 ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc
    21841 accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac caatagtgac
    21901 gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc agcgggaaat
    21961 ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg
    22021 ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg ggttacccat
    22081 gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtgat
    22141 gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc
    22201 agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacaat
    22261 ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt
    22321 tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc tacgctaaca
    22381 agaaaattac caccaatttt attaacatta taatttcaat aaatttacca gccagaagtg
    22441 cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt ttaaatttat
    22501 ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt
    22561 taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga
    22621 tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga
    22681 actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg ccattttctc
    22741 gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc tggtgaacgt
    22801 acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag gcatcattac
    22861 cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga cggcgatccc
    22921 ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc tgaaaatccc
    22981 caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga cgttgaccat
    23041 gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg tctctaaaat
    23101 cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DAJTVE010000063        22385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000063 DAJTVE010000000
VERSION     DAJTVE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..1134
                     /gene="ompC"
                     /locus_tag="OO439_002279"
     CDS             28..1134
                     /gene="ompC"
                     /locus_tag="OO439_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6316634.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1246..2301
                     /gene="apbE"
                     /locus_tag="OO439_002280"
     CDS             1246..2301
                     /gene="apbE"
                     /locus_tag="OO439_002280"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6316635.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2375..3439
                     /gene="ada"
                     /locus_tag="OO439_002281"
     CDS             2375..3439
                     /gene="ada"
                     /locus_tag="OO439_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6316636.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3439..4089
                     /gene="alkB"
                     /locus_tag="OO439_002282"
     CDS             3439..4089
                     /gene="alkB"
                     /locus_tag="OO439_002282"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6316637.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4165..5808
                     /gene="yojI"
                     /locus_tag="OO439_002283"
     CDS             4165..5808
                     /gene="yojI"
                     /locus_tag="OO439_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6316638.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6026..7672
                     /gene="mqo"
                     /locus_tag="OO439_002284"
     CDS             6026..7672
                     /gene="mqo"
                     /locus_tag="OO439_002284"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6316639.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7981..8469)
                     /gene="eco"
                     /locus_tag="OO439_002285"
     CDS             complement(7981..8469)
                     /gene="eco"
                     /locus_tag="OO439_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6316640.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            8877..9371
                     /gene="napF"
                     /locus_tag="OO439_002286"
     CDS             8877..9371
                     /gene="napF"
                     /locus_tag="OO439_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6316641.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9361..9624
                     /gene="napD"
                     /locus_tag="OO439_002287"
     CDS             9361..9624
                     /gene="napD"
                     /locus_tag="OO439_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6316642.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9621..12107
                     /gene="napA"
                     /locus_tag="OO439_002288"
     CDS             9621..12107
                     /gene="napA"
                     /locus_tag="OO439_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6316643.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12114..12809
                     /gene="napG"
                     /locus_tag="OO439_002289"
     CDS             12114..12809
                     /gene="napG"
                     /locus_tag="OO439_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6316644.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            12796..13659
                     /gene="napH"
                     /locus_tag="OO439_002290"
     CDS             12796..13659
                     /gene="napH"
                     /locus_tag="OO439_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6316645.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13656..14105
                     /gene="napB"
                     /locus_tag="OO439_002291"
     CDS             13656..14105
                     /gene="napB"
                     /locus_tag="OO439_002291"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6316646.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14115..14717
                     /gene="napC"
                     /locus_tag="OO439_002292"
     CDS             14115..14717
                     /gene="napC"
                     /locus_tag="OO439_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6316647.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14736..15353
                     /gene="ccmA"
                     /locus_tag="OO439_002293"
     CDS             14736..15353
                     /gene="ccmA"
                     /locus_tag="OO439_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6316648.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15350..16012
                     /gene="ccmB"
                     /locus_tag="OO439_002294"
     CDS             15350..16012
                     /gene="ccmB"
                     /locus_tag="OO439_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6316649.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16054..16791
                     /gene="ccmC"
                     /locus_tag="OO439_002295"
     CDS             16054..16791
                     /gene="ccmC"
                     /locus_tag="OO439_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6316650.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16788..16997
                     /gene="ccmD"
                     /locus_tag="OO439_002296"
     CDS             16788..16997
                     /gene="ccmD"
                     /locus_tag="OO439_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6316651.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            16994..17473
                     /gene="ccmE"
                     /locus_tag="OO439_002297"
     CDS             16994..17473
                     /gene="ccmE"
                     /locus_tag="OO439_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6316652.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17470..19413
                     /gene="ccmF"
                     /locus_tag="OO439_002298"
     CDS             17470..19413
                     /gene="ccmF"
                     /locus_tag="OO439_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6316653.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19410..19967
                     /gene="dsbE"
                     /locus_tag="OO439_002299"
     CDS             19410..19967
                     /gene="dsbE"
                     /locus_tag="OO439_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6316654.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            19964..21016
                     /gene="ccmH"
                     /locus_tag="OO439_002300"
     CDS             19964..21016
                     /gene="ccmH"
                     /locus_tag="OO439_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6316655.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21051..21698)
                     /gene="narP"
                     /locus_tag="OO439_002301"
     CDS             complement(21051..21698)
                     /gene="narP"
                     /locus_tag="OO439_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6316656.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22018..>22385
                     /locus_tag="OO439_002302"
     CDS             22018..>22385
                     /locus_tag="OO439_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6316657.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         4992 a         5638 c         6387 g         5368 t
ORIGIN      
        1 aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct cctggtccca
       61 gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga cggcaacaaa
      121 ttagatctgt acggtaaagt agacggcctg cactatttct ctgacaacaa gtcagaagac
      181 ggcgaccaga cctatgtacg tcttggtttc aaaggcgtaa ctcaggttac tgaccagctg
      241 accggttacg gccagtggga atatcagatc cagggcaata cctctgaaga caacaaagaa
      301 aactcctgga cccgtgtggc attcgcaggt ctgaaattcc aggatgtggg ttctttcgac
      361 tacggtcgta actacggcgt tgtttacgac gtaacttctt ggaccgacgt actgccagaa
      421 ttcggtggcg acacctacgg ttctgacaac ttcatgcagc agcgtggtaa cggcttcgcg
      481 acctaccgta acactgactt cttcggtctg gttgacggcc tgaactttgc tgttcagtac
      541 cagggtaaaa acggtagcgt aagcggcgaa ggcatgacca acaacggtcg tggtgctctg
      601 cgtcagaacg gcgacggcgt tggcggatct atcacttatg attacgaagg cttcggtatc
      661 ggtggtgcga tctccagctc caaacgtact gatgatcaga acagcccgct gtacatcggt
      721 aacggcgacc gtgctgaaac ctacaccggt ggtctgaaat acgacgctaa caacatctac
      781 ctggctgctc agtacaccca gacctataac gcaactcgcg taggttccct gggttgggcg
      841 aacaaagcac agaactttga agctgttgct cagtaccagt tcgacttcgg tctgcgtccg
      901 tctgtagcat acctgcagtc taaaggtaaa aacctgggta ccatcgctgg tcgtaactac
      961 gacgacgaag atatcctgaa atatgttgat gttggtgcta cctactactt caacaaaaac
     1021 atgtccacct acgttgacta caaaatcaac ctgctggacg acaaccagtt cactcgtgac
     1081 gctggcatca acactgataa catcgtagct ctgggtctgg tttaccagtt ctaatctcga
     1141 ttgatatcga acaaagggcc tgcgggccct tttttcattg ttttcagcgt acaaactcat
     1201 ttttttggtg tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt
     1261 acccgcgtgg cactgctggc tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa
     1321 cccgccaaaa cccacgctac tgaagttacc gttctagaag gcaaaaccat gggtaccttc
     1381 tggcgtgcca gcatcccggg cattgatgcc aaacgcagtg ccgaacttaa agaaaagatt
     1441 cagacccagc tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg
     1501 atgcgcttta acgattcgca aagtttatcg ccgtggccgg taagtgaagc gatggccgat
     1561 atcgtcacca cctcgctgcg cattggcacg aagaccgatg gcgcgatgga tataaccgtc
     1621 gggccgctgg tgaatctgtg gggctttggc ccggaacaac agtcggttca aattccgagc
     1681 caggaacaga tcgatgcgat gaaagccaaa accggcttac agcacctgac ggtcattaat
     1741 cagtcgcatc agcaatatct gcaaaaagac ctgccggatt taaatgtcga tctctccacc
     1801 gtcggtgaag gttatgcggc ggatcacctg gcacgcttga tggagcagga agggatttcc
     1861 cgctatctgg tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgaaggcctg
     1921 ccgtggcggg tagcgattca aaaaccaacc gataaagaaa acgcggttca ggctgtggtg
     1981 gatatcaacg gtcatgggat tagcacctct ggcagctacc gtaactatta cgaactggac
     2041 ggcaaacgtc tttcccatgt tatcgatccg caaaccgggc gtcccatcga acacaatctg
     2101 gtatccgtga cggtgattgc tccgacggcg ctggaagccg atgcctggga tactggcttg
     2161 atggtactcg ggccggagaa agccaaagag gttgttcgcc gggaagggct ggcggtttat
     2221 atgatcacca aagaaggcga tagctttaaa acctggatgt caccacagtt taaaagcttc
     2281 cttatcagcg aaaaaaatta aagcgcaaga ttgttggttt ttgcgtgatg gtgaccgggc
     2341 agactaaagg ctatccttaa ccagggagct gattatgaaa aacgccacat gcttaactga
     2401 cgatcaacgc tggcaatctg tcttagcccg cgacccgaat gccgacggcg aattcgtttt
     2461 cgccgtgcgc accacgggta tcttttgccg tccgtcttgc cgcgccagac atgccttgcg
     2521 ggaaaatgtc tccttctacg caaatgccag cgaggcactc gccgccggat ttcgcccctg
     2581 caaacgttgt cagccagaca aagccaatcc ccggcaacat cggttggata aaatcaccca
     2641 cgcgtgtcga ctgctggaac aggaaacgcc tgtaacgctg gaagccttag ccgaccaggt
     2701 ggcgatgagt ccatttcatc tacatcggtt gtttaaagcg actaccggaa tgacgcctaa
     2761 agcctggcaa caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag
     2821 cgtgacgacg tctattctta acgccggatt ccccgacagc agcagttact accgcaaagc
     2881 cgatgaaacg ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt
     2941 gcgttacgcg ctggctgatt gtgagctggg tcgttgcctg gtggcagaaa gcgagcgggg
     3001 gatttgcgcg atattgctgg gcgatgatga cgcgacatta atcagcgagt tgcagcagat
     3061 gtttcccgct gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt
     3121 gatcgccagc ctcaatcaac gcgatacgcc gctgacgtta ccgctggaca ttcgcggcac
     3181 tgcttttcag caacaagtct ggcaggcact gcgcacgata ccttgtggtg aaaccgtcag
     3241 ttatcagcaa ctggctaacg ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc
     3301 ctgtgccgcc aacaagctgg ctatcgtaat accctgtcat cgagtggtcc gtggtgatgg
     3361 cacactttcc ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc
     3421 tgaaaatgag gagaggtaat gttggatctg tttgccgatg ctgaaccgtg gcaagagcca
     3481 ctggcggctg gtgcggtaat tttacggcgt tttgctttta acgctgcgga gcaactgatc
     3541 cgcgatatta atgacgttgc cagccagtcg ccgtttcgcc agatggtcac ccccggggga
     3601 tataccatgt cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa
     3661 ggttatctct attcgcccat tgatccgcaa acaaataaac cgtggcccgc catgccacaa
     3721 agctttcatg atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca
     3781 gatgcttgtc ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa
     3841 gacgaaccgg atctgcgcgc gccgattgtt tctgtttctc ttggcttacc cgcgattttt
     3901 caatttggtg gcctgaaacg taatgatccg ctcaaacgtt tgttgttgga acatggcgat
     3961 gtggtggtat ggggcggtga atcgcggctg ttttatcacg gcattcaacc gctgaaagcg
     4021 gggtttcatc cactcaccgg cgactgccgt tacaacctga cattccgtca ggcaggtaaa
     4081 aaagaataaa aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg
     4141 taattcattg tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc
     4201 tggccattta tcagtgtgat ggagctaagc ctcgccagtg cggctttagg cattggctta
     4261 attgctttta tcaatcagcg ccttatcgaa acggcggata ccagtctgct ggtgttgccg
     4321 gagtttctgg gattattgct gctgttgatg gcaatcactc tcggatcgca actggcgctc
     4381 accactttgg ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctc
     4441 gatacccacg tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc gggattaacc
     4501 agcgatgtgc gcaatatcac cattgctttt gtgcgtctgc cggaactggt gcaggggatc
     4561 attctcacta tcggttcagc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta
     4621 acggctatct ggatggcggt caccatctgg ggcggttttg tgctggtggc gcgggtgtac
     4681 aaacatatgg cgaccctgcg tgaaaccgaa gataagctgt acacggattt tcaaaccgta
     4741 ctcgaagggc gcaaagagct gactctgaac cgtgaacgcg ccgagtatgt gtttaacaac
     4801 ctctacattc ctgatgcgca agagtatcgc catcatatta tccgcgcaga caccttccat
     4861 cttagtgccg tgaactggtc aaacatcatg atgctgggcg ctatcggcct ggtgttctgg
     4921 atggcgaaca gcctcggttg ggctgatacc aacgttgctg cgacctattc gttgacgctg
     4981 ttattcctgc gtacgccgct gctttcggcg gttggcgcat tgccgacgct gctgacggcg
     5041 caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg
     5101 cgcccgcagg cgtttcccaa ctggcaaacg ctggagctgc gtaatgtgac gtttagctat
     5161 caggataacg cgttttccgt tgggccgatt aacctcacca ttaaacgtgg cgagctgctg
     5221 tttctgattg gtggcaacgg tagtggaaaa tcgacgctgg cgatgttgct gacgggcttg
     5281 taccagccac aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg
     5341 gaagattatc gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg
     5401 ctggggccgg aggggcaacc cgctaacccg caactggttg agaagtggct ggcgcagctg
     5461 aaaatggctc ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa
     5521 gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg
     5581 ctggatgaat gggcggcgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg
     5641 ctgccgctga tgcaggagat gggtaaaact attttcgcca tcagtcatga tgatcattac
     5701 tttatccacg ccgaccgcct gctggaaatg cgcaatgggc aacttagcga gctgacgggc
     5761 gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct
     5821 gacctggcgg actgctgccg ccaggtcaat attctaaaaa tcccttcatt tctaataacc
     5881 ctataattaa tcaacgaaat tataatgttt ctaaaattag aatataattt ataaacatta
     5941 tttaaatgtt gttactttag tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag
     6001 cgactaaaag taaggcatta acaagatgaa aaaagtgact gccatgctct tctcgatggc
     6061 cgtggggctt aatgccgttt cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac
     6121 tgatgtactg ttgattggcg gcggcattat gagcgccacg ttggggacct atttacgcga
     6181 gctggagcct gaatggtcga tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag
     6241 ttcgaacggc tggaacaacg ccggaaccgg gcattctgca ctgatggaac tgaactacac
     6301 cccgcaaaac gccgatggca gtatcagtat tgaaaaagca gtcgccatta acgaagcatt
     6361 tcagatttcc cgccagttct gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg
     6421 ttcatttatc aataccgttc cgcatatgag ctttgtctgg ggcgaggata acgttaattt
     6481 cctgcgcgcc cgttacgccg cgttgcaaca aagctcgctg tttcgcggta tgcgttactc
     6541 tgaagatcac gcgcagatca aagagtgggc accgttagtg atggaagggc gcgatccgca
     6601 acagaaagtg gcagccacgc gtacggaaat tggtaccgat gtgaactacg gcgagatcac
     6661 ccgccagtta attgcttcct tgcagaagaa atctaacttc tcgctgcaac tcagcagcga
     6721 agtccgcgcc ctaaagcgta atgacgataa cacctggacc gttaccgttg ccgatctgaa
     6781 aaatggcact gcacagaaca ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc
     6841 gctgaagctg ttacaggaat cggggattcc ggaagcgaaa gactacgccg gtttcccggt
     6901 gggcggacag ttccttgttt cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt
     6961 ttacggtaaa gcatccgttg gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt
     7021 tctggacggt aaacgcgtag tgctgtttgg gccatttgcc accttctcaa ccaaattcct
     7081 caaaaacggt tcattgtggg atctgatgag ttccaccacc acctctaacg tgatgccgat
     7141 gatgcacgtc gggctggata atttcgatct ggtgaaatac ttggtgagtc aggtgatgtt
     7201 gagtgaagag gatcgttttg aagcgttgaa agagtactat ccgcaggcga aaaaagagga
     7261 ctggcgtttg tggcaagcgg ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg
     7321 tggtgtactg cgtctgggta ctgaagtggt cagtgaccag caagggacca ttgccgcgct
     7381 gctgggggca tcgccagggg cgtcaaccgc tgcgccgatt atgttggatc tgctggaaaa
     7441 agtatttggc gatcgcgttt ccagcccgca atggcaggct acgttgaaag cgatcgttcc
     7501 atcttatgga cgcaagctga acggtgatgt agcggcaaca gaacgcgagt tgcagtacac
     7561 cagcgaagtg ctgggcctga aatacgacaa gccgcaggca gcagatagta cgccgaaacc
     7621 gcagttgaaa ccgcaacccg ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc
     7681 cacatccggc atggtatgcc ggatgtggcg taaccgcctt atccgaccta cggggtggag
     7741 cacttcacag cggtgtaggc ctgataagac gcgtcagcgt cgcatcaggc aaccggctcg
     7801 ggcgttagat gtcggatgcg gcgtaaccgc cttatccaac ctacggggtg gagcacttca
     7861 cagcggtgta ggcctgataa gacgcgccag cgtcgcatca ggcaaccggc ttgggcgtta
     7921 gatgtcggat gcggcgtgac cgccttatcc gacctacggg gtgacgcact tcacagcagt
     7981 ttagcgaact accgcgttgt caattttctc ttccgccttc cagacgcggt acttcacatc
     8041 tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca gcattccagc
     8101 atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc aggccatcat
     8161 cgtggaaacc ggcgagctaa ctttatcaaa gacataataa tcatagcccc agccttccag
     8221 cgttttgttt tccagcttcc cgccgagacg atgcaaattg caatcgactt ccagcgtctg
     8281 accggttaac agttctactt tcagggtaga ttcatcttct tgcggggtta actgaatcac
     8341 ctgacgcttc atcccttttt cagcttgtgg ataaggcgcg attttttcca gtggctggac
     8401 gctttctgcc gcccaggcgg aagtggtggc gaaagcggca aacaatactg caggtagaat
     8461 ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga tgtcgctgaa
     8521 ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag aacattcaga
     8581 caattttacc tgctctttta gtgttaaatt ctgatgaggg agcaaaccag cagaaatatc
     8641 tctggtgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc
     8701 cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg
     8761 gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta
     8821 tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga
     8881 agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta
     8941 tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc cgttgcgacg
     9001 cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg
     9061 ttaatttcaa aaataacgag tgctgcttct gttacgcctg cgcgcaggct tgccccgaat
     9121 cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt ggggacgcgt
     9181 gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa
     9241 ttatctttcg cccgacgctc tccgggatct accagccgca acttaatagc caactttgca
     9301 acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc
     9361 atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca
     9421 gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg
     9481 agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt
     9541 gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa
     9601 gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg
     9661 ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg
     9721 gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg
     9781 gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac
     9841 cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta
     9901 aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg
     9961 aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg
    10021 ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct
    10081 gggaaggtta tgccgcgtcc aagctgttta aagcgggctt ccgttcgaac aacatcgacc
    10141 cgaacgcgcg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg
    10201 atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg
    10261 caaacatggc ggagatgcac ccgatcctct ggtcgcgcat cactaaccgt cgtctctcta
    10321 atcagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg
    10381 ataacggcat catctttacg ccacagtctg acctggtgat cctgaactac atcgccaact
    10441 atatcattca aaacaatgcg ataaatcagg acttcttcag caaacacgtt aacctgcgca
    10501 aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga
    10561 agaatccagg ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg
    10621 ccgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac cagttagaac
    10681 aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct
    10741 tcaaccagca tactcgtggc gtgtgggcta acaacctggt ctacaacctg cacctgctga
    10801 ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcaa ccttctgcgt
    10861 gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga
    10921 ccaacgagaa acaccgtgat atctgcgaga agaagtggaa tatcccgagc ggcaccattc
    10981 cggcgaaaat cggtctgcac gcggtggcac aagaccgtgc gctgaaagac ggcaaactga
    11041 atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc aatgaagagc
    11101 gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag
    11161 tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggtg
    11221 cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag gcaccgggcg
    11281 aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag
    11341 tgtggccgga agagctgcta gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag
    11401 tgctttatgc cacaccggaa gtgagcaaat tcccggtatc cgaactggcg gaagatcagc
    11461 tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg
    11521 catggtttgg tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc
    11581 gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta
    11641 acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg gatggtaaag
    11701 cggtgatctt cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc
    11761 tgtggctctc taccggacgc gttctggagc actggcacac tggcagtatg actcgccgtg
    11821 tgccggaact gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg ctggatgcga
    11881 aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga
    11941 tctcgattgt tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt
    12001 tcttcgacgc cgcacagctg gttaacaaac taacgctgga cgcgaccgat ccgctctcga
    12061 aagagacgga cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc
    12121 ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgttgtt cgcacagcag
    12181 gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg
    12241 gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc
    12301 gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggctt
    12361 ctggcctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg
    12421 aggacattcc gtgcgccaaa gtgtgcccga gtggtgcgct ggatcgtgag atcgaatcga
    12481 tcgacgacgc gcggatgggg ctggcggtgc tggtggacca ggaaaactgt ctcaactttc
    12541 aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc
    12601 tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca
    12661 gcgacgcctg taccggctgc ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa
    12721 tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggct
    12781 ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggtgaaa
    12841 aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg
    12901 ctggggatgt ttttgagcgg tccgtggttt ggtgtgtgga tcttacacgg caactacagc
    12961 tgtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt
    13021 gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgataat cactgtgctc
    13081 tacgccctgg caggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc
    13141 gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc
    13201 cacattcgct acgtgctgtt ggtggtcatt ctgctgggat cagccttaac cggcacgctc
    13261 atctgggaat ggatcaaccc cgtttccctg atggggcgta gcctggtaat gggcttcggc
    13321 agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc
    13381 tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg
    13441 attaccgttg cggccagcga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt
    13501 tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc
    13561 accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt
    13621 acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag
    13681 cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta
    13741 atggcgtcga ttttagccag tcgccggaag tatccgggac gcaggaaggg gccattcgga
    13801 tgccaaaaga gcaggatcgg atgccgctga actatgtgaa tcagccgcca atgatcccgc
    13861 atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg
    13921 tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg
    13981 acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac
    14041 cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga
    14101 aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga
    14161 aatggtggcg tacccccagc cgtctggcgc tgggaacgct gctgttgatc ggttttgtcg
    14221 gcggcatcgt cttctggggc ggctttaaca ccggaatgga aaaagccaat accgaagagt
    14281 tctgcattag ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg gattccgtgc
    14341 actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt
    14401 ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg
    14461 gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacaatggca cagaatgagt
    14521 ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata
    14581 tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg
    14641 ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag
    14701 tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt
    14761 gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt
    14821 gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg
    14881 ggttgtctcg ccctgacgca ggcgaggttc tctggcaagg gcagcccttg catcaggtac
    14941 gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc
    15001 tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg
    15061 aagccctggc gcaggccggg cttgccggat tcgacgatat tccggtaaat caactttctg
    15121 ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga
    15181 tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta
    15241 tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg
    15301 ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca tgatgttctg
    15361 gcgcattttc cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc
    15421 gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca
    15481 actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct
    15541 ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt
    15601 gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac
    15661 cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa tggatgttta
    15721 tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg gctttctcgg
    15781 tgcaccgggt gtggcgttga cagtgggact taagcgcggt ggtgtgctgc tcagcatact
    15841 ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc
    15901 catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc
    15961 gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat
    16021 tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg
    16081 atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt
    16141 gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag
    16201 cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc
    16261 atttatgcat caatggcagt ggcagcgttt attggccttg tctggcagat gaaaatggcc
    16321 aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt
    16381 accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg
    16441 acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc
    16501 gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat
    16561 ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg
    16621 atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc
    16681 ctgctcctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa
    16741 aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat
    16801 ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg
    16861 tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg caacatcgcg
    16921 caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac
    16981 agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt
    17041 ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct
    17101 cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt
    17161 cggtcagcgt ttgcgcgttg gcgggatggt gatgctgggt agtgtgcagc gcgatcccaa
    17221 ttcgctgaaa gtgaccttca ctatttacga tgctgaaggc tcagtggatg tctcttacga
    17281 aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga
    17341 aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc
    17401 gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga
    17461 cccagcatca tgatgccgga aattggaaac gggctgctgt gtctggcgtt agggattgcg
    17521 ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg catgatcgcg
    17581 tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg
    17641 gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcta ctccaatacc
    17701 cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctactg
    17761 ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt
    17821 ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg
    17881 ctgttcattc tctttacctc taacccattc tcacgcacgc tgccgaactt cccgattgaa
    17941 gggcgcgatc ttaacccgct gttacaggac ccggggctga tcttccatcc gcctctgctt
    18001 tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc tttgctgagc
    18061 gggcgtctgg acagcactta tgcgcgtttt actcgcccgt ggacgctggc ggcgtggatt
    18121 ttcctgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggg
    18181 ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact
    18241 gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta
    18301 ctgctggcaa tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc
    18361 gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc
    18421 tttatggtgc tggtgattgg aggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt
    18481 cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt
    18541 ttgctggtcg ccgcgatgct ggtggtgttg ctggggacgc tgctgccgct ggtgcacaag
    18601 caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg
    18661 ctgatggtgc cgtttgcgct actgctgggt gtcggtcctc tggtgcgctg ggggcgcgat
    18721 cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtct
    18781 ctgctgttgc cgtggctgtt tgagagcaaa gttgtggcga tgacggtgct cggcctggca
    18841 atggcctgct ggattgcggt gctggcaatt gcggaagctg cgctgcgtat ttcacgcggc
    18901 acgaaaacca ccttcagtta ttgggggatg gtggctgctc acctggggct ggcagtgaca
    18961 attgttggca ttgcttttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc
    19021 ggcgatagca tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact
    19081 ggcccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgagaggg taaaccggaa
    19141 acagtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg
    19201 gcgattgacg gtggcatcac gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac
    19261 ggcgcgtggg ctgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg
    19321 ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg
    19381 agtccgcaaa aaactgcgcc ggaggctgta tgaagcgcaa agtattgtta attccgttga
    19441 ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg
    19501 atgatccgac caatctggaa tcggcgctca ttggcaagcc agtgccgaag tttcgcctcg
    19561 aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag ggcaaaccag
    19621 tgctgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga
    19681 atcagctttc tgcgcagggc atccgcgtag tcggcatgaa ctataaagac gatcgccaga
    19741 aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg
    19801 atggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaacgttc cttattgacg
    19861 gcaacggcat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag
    19921 agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg
    19981 catgctgatg ctgatgatct ccggctcagc gctggcgacc atagacgtgt tgcagtttaa
    20041 agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg
    20101 ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc gccagaaagt
    20161 gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg
    20221 ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt
    20281 gctgccagta gtagctattg gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg
    20341 ggtacgcgtg gtgccggaag cgtttcctga acaaagcgtg ccggaaggta agcgtgccgg
    20401 atatgtcgct tatttgccgg gcattgtggt ggcgttaatt gtggctggcg tcagctacta
    20461 ccagaccgga aattatcaac aggtgaaaat ctggcagcag gccacggcac aggctccggc
    20521 gttgctggac agggcgctgg atccgaaagc tgatccgctc aacgaagaag agatgtcgcg
    20581 ccttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag acggctggat
    20641 tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg atgcatacgc
    20701 tactgcgtat cgcctcgatc cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt
    20761 gacacgttca tctgatccca acgacaaccg cctcggcggc gaactgctac gtcagctggt
    20821 gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg cctttgagca
    20881 gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa
    20941 cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc
    21001 gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc
    21061 ccgcgttgtt gcaggaacag aatggtggcc gccacgcgtg agcggacatt gagtttacgc
    21121 agcagatttc gaatatgaac ttttactgtc tgctcggaaa tattcaacac agaggcaatc
    21181 tgtttatttg acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc
    21241 acgctgaagg gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg
    21301 cgttcgctaa agactttgct gcctttcgct ccggcacgaa tcgcttccag caacacttcc
    21361 gggtcgctgt ctttcagcag ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg
    21421 ctggaggcat cggatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag
    21481 gcattgaggg tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg
    21541 atatccagtc tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact
    21601 tcaaagccag gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca
    21661 tccacaatca tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt
    21721 agaatcatta tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca
    21781 ttttatgtat ggacatgtag tgaaaactac aaccttgccg ttcgctttgg catcattttc
    21841 attataaaga cggaattttg catcagcttc tcatcaagaa aaacaactaa aaaatcaatc
    21901 acaatacttg tgcatttctt taaacagttt ttaaaaactg actccgggta tggagctatg
    21961 ggtattttct gtacccaatg cttttaacag caattaattt cataggatga aagctcaatg
    22021 catcaatctg gttctgtttc tctttgtcgt tccgcaatat ctgttctggt ggctacagcg
    22081 ttatattcac ccatagcatt ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt
    22141 ggcctggaaa aagatatcca gctggtttat gggaccgcca ataatacgaa aatcaatcct
    22201 ggcggagaac agcatataaa agaatttggt gtaagtagta atactgaaat taacggcggg
    22261 tatcagtaca ttgaaatgaa tggcactgca gaatactcag tattaaatga tggttatcaa
    22321 attgttcaaa tgggtggcgc ggtaaaccag actacgctca ataatggagt gctacaggtt
    22381 tatgg
//
LOCUS       DAJTVE010000064        22133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000064 DAJTVE010000000
VERSION     DAJTVE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..525)
                     /locus_tag="OO439_002303"
     CDS             complement(<1..525)
                     /locus_tag="OO439_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6316658.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            821..1597
                     /gene="exuR"
                     /locus_tag="OO439_002304"
     CDS             821..1597
                     /gene="exuR"
                     /locus_tag="OO439_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6316659.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1942..2604
                     /locus_tag="OO439_002305"
     CDS             1942..2604
                     /locus_tag="OO439_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6316660.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2608..2991
                     /gene="mzrA"
                     /locus_tag="OO439_002306"
     CDS             2608..2991
                     /gene="mzrA"
                     /locus_tag="OO439_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6316661.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3138..3506
                     /locus_tag="OO439_002307"
     CDS             3138..3506
                     /locus_tag="OO439_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6316662.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3544..3849
                     /locus_tag="OO439_002308"
     CDS             3544..3849
                     /locus_tag="OO439_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6316663.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3852..4256
                     /locus_tag="OO439_002309"
     CDS             3852..4256
                     /locus_tag="OO439_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6316664.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4246..4545
                     /locus_tag="OO439_002310"
     CDS             4246..4545
                     /locus_tag="OO439_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6316665.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4731..5123
                     /locus_tag="OO439_002311"
     CDS             4731..5123
                     /locus_tag="OO439_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6316666.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5193..6206
                     /gene="yqjG"
                     /locus_tag="OO439_002312"
     CDS             5193..6206
                     /gene="yqjG"
                     /locus_tag="OO439_002312"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6316667.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6471..6836
                     /locus_tag="OO439_002313"
     CDS             6471..6836
                     /locus_tag="OO439_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6316668.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7078..7434
                     /locus_tag="OO439_002314"
     CDS             7078..7434
                     /locus_tag="OO439_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6316669.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO439_002315"
                     /pseudo
     CDS             complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO439_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8486..9187
                     /locus_tag="OO439_002316"
     CDS             8486..9187
                     /locus_tag="OO439_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6316670.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9210..9374
                     /locus_tag="OO439_002317"
     CDS             9210..9374
                     /locus_tag="OO439_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316671.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9508..10818)
                     /locus_tag="OO439_002318"
     CDS             complement(9508..10818)
                     /locus_tag="OO439_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6316672.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10846..12177)
                     /locus_tag="OO439_002319"
     CDS             complement(10846..12177)
                     /locus_tag="OO439_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6316673.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO439_002320"
     CDS             complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO439_002320"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6316674.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13888..14277)
                     /locus_tag="OO439_002321"
     CDS             complement(13888..14277)
                     /locus_tag="OO439_002321"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6316675.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO439_002322"
     CDS             complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO439_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6316676.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO439_002323"
     CDS             complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO439_002323"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6316677.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO439_002324"
     CDS             complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO439_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6316678.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO439_002325"
     CDS             complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO439_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6316679.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO439_002326"
     CDS             complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO439_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6316680.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21421..21765
                     /gene="tdcR"
                     /locus_tag="OO439_002327"
     CDS             21421..21765
                     /gene="tdcR"
                     /locus_tag="OO439_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6316681.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5801 a         5650 c         5169 g         5513 t
ORIGIN      
        1 gcgttgattc cctgcattaa ctaatttttg ggcagcattt acttctaact tagcaatagg
       61 ttgctgaggt ggaacactaa caacaatccc ccaaattaag ttaactgtta actgcataga
      121 gagatctttt ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg
      181 ataatttttt tctttttgct ctggttccat tgcaacaaag cgcacagttt tgctactacc
      241 ggctggtaaa gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat
      301 ctcaacttca ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat
      361 gtactgaact tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat
      421 ggaaacagcc ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa
      481 taaaagcggc gtaagccgtg ataagagatt aattttcgcc accatataaa tttgatctta
      541 caatattgtt tccaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa
      601 aaaagcattt ttagttaata ttaaatttaa aggctgggaa ttaacttact gaaatttagt
      661 agctttcaca atgacgatac ttatttttta ggaatttata aatattcaca atattaattc
      721 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
      781 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      841 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      901 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      961 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
     1021 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
     1081 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
     1141 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
     1201 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
     1261 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
     1321 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
     1381 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
     1441 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
     1501 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
     1561 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
     1621 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
     1681 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1741 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1801 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1861 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
     1921 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
     1981 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
     2041 gtaattttgt tccttgaaaa cggcttgctt ccagcggcct ttttaccggg cgacagttta
     2101 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
     2161 ctgctgaccg ttgccgccag cctcggctgc tgggtcagct atattcaggg gcgatggctg
     2221 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     2281 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgcg
     2341 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     2401 cagtttttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     2461 atgctcggca aaacgccgat atttttaaag tacgaggacc agctgatgtc atgcctgatg
     2521 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     2581 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     2641 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     2701 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     2761 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     2821 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     2881 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     2941 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     3001 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     3061 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca
     3121 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     3181 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     3241 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     3301 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     3361 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     3421 gcgatgccga taagattgcc aaacgcgaac ggaaactggc agaagcgcag gaagagctga
     3481 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     3541 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     3601 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     3661 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     3721 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     3781 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     3841 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     3901 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     3961 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     4021 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     4081 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     4141 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     4201 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     4261 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     4321 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     4381 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     4441 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     4501 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     4561 agcaattccg gccttatccc tcaaattttt tgaagatttt tgacagtttt ccttgctaac
     4621 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     4681 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     4741 tagaagatgt tggtgtactg gtagcgcgca ttttaatgcc gattctgttt attaccgcag
     4801 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     4861 cgggttttat gctgccgctg gtgattctgc ttgagttcgg tggtggtctg gcaatcctgt
     4921 tcggtttcct gactcgcacc acagccctgt ttactgcggg ctttacgctg ctgacggcat
     4981 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     5041 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     5101 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     5161 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     5221 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     5281 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     5341 cagagaaaga tcgttatcat ctctatattt cactcgcctg cccgtgggcg caccgcacgc
     5401 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     5461 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     5521 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     5581 gagttactgt tcccgtactg tgggacaaaa agaaccacac catcgtcagc aacgaatcaa
     5641 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcgaaa gcgggtgatt
     5701 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     5761 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     5821 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     5881 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     5941 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     6001 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     6061 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     6121 caattggtcc gtggcaggat ctcgatgaac cgcaggacga gatgttcgct tcggttaaaa
     6181 aaaggcacct tacgaagtgc ctctgataat tagctaagtg catgttgttc atatcggatg
     6241 cggcgtgaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
     6301 gcaggccaga taagcgtagc gcatctggcg attttgcgct tgtcatcagt cttagcgcgc
     6361 cttcacagca ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     6421 tcttaatttg ctgctttaaa aacaagtgat tgagcaacat tgaggcaaaa atggactggt
     6481 atctgaaagt aataaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact
     6541 ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg gataaaatgt
     6601 taggctggca acgcgctggt ggcgaaggca tcctgacgac aatctacggt attctggtgt
     6661 ttttaccgtg gtgggcagtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt
     6721 gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc tttaactgcc
     6781 aggcgggtac gccaggcgaa aaccgctttg gacctgatcc aaaactggaa ccttaaaaca
     6841 aatctgctcg ccggatgtct gactcatccg gcttttatca tcatgttgat gaatgaataa
     6901 tttactaatg tttatttaaa atatttcaat gtcgttatat aaattaccta taaaaaataa
     6961 ccatgtattt atcttaaata tcaaaacctt tataacatcc gaaaaagaaa gatacgaata
     7021 gcatttacac aatacccgga atagactttc gtcactttta attaaaggga tgtttttatg
     7081 cagtggtatt tatccgtatt aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa
     7141 gagtactgga tgtttactct gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa
     7201 ttgattttag gtctggagct tccatatctg tctatgcttt atttgctggc aaccttcctt
     7261 cccgtccttg cgcttgctat ccgtcgctta cacgataccg accgttcagg tgcctgggcc
     7321 ttgctctttt ttgttccgtt tattggctgg ctcgtgcttc tggttttttt ctgcacagaa
     7381 ggtacttctg gcagcaatcg ttacggaaac gatccgaagt ttggttcaaa ttaatcttag
     7441 aattggggcg atatttcgcc cctttttatt aacaataata aatattattt tccgctaaaa
     7501 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
     7561 gccataataa tatcgatctc actggtcgat tccgggctga cgacacgcaa ccgcccttcc
     7621 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
     7681 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagttgt
     7741 acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg atatttcacg
     7801 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
     7861 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
     7921 atatccggcg cgataacaat atccgcccgc ccctgctcca gtcgttccta cgcccccgcc
     7981 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
     8041 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
     8101 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
     8161 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
     8221 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
     8281 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
     8341 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
     8401 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
     8461 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     8521 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     8581 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
     8641 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     8701 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
     8761 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
     8821 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
     8881 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta cgatgggaag
     8941 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     9001 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     9061 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     9121 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     9181 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     9241 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     9301 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     9361 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     9421 atccaacaac attaacgaat taaacaaccc gtaggccgga taaggtgcgc cagcatcgca
     9481 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     9541 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     9601 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     9661 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     9721 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     9781 agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     9841 cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     9901 atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac
     9961 aaccaccaca ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc
    10021 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
    10081 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
    10141 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
    10201 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
    10261 cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc
    10321 ctgctgggta aacaccacac cattgtgcgt ctcaatacgc acaatgttgg tatgcccgcc
    10381 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
    10441 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
    10501 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
    10561 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
    10621 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
    10681 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
    10741 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
    10801 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
    10861 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
    10921 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
    10981 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatagc
    11041 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
    11101 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
    11161 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
    11221 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
    11281 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgc cccatcgcca gcgtgaacga
    11341 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
    11401 tgctttatgc cgcgccactt caatagattt ttcccgcgag cgataagaga tcaccatcgg
    11461 gcttaacgtc tggataaaca aaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    11521 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    11581 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
    11641 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    11701 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    11761 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    11821 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    11881 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    11941 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    12001 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    12061 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    12121 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcga tttccataat
    12181 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    12241 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    12301 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
    12361 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    12421 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
    12481 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    12541 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccacgcgg
    12601 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    12661 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    12721 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
    12781 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    12841 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    12901 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    12961 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    13021 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    13081 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
    13141 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aatcctgcgt
    13201 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    13261 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    13321 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    13381 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    13441 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    13501 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    13561 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    13621 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca
    13681 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    13741 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    13801 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    13861 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    13921 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    13981 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    14041 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    14101 cgctttgacg ttttcgaggc ttaaacacgc ctgatcttgc acatcagccg ggatctcacc
    14161 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    14221 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
    14281 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    14341 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    14401 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    14461 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    14521 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    14581 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    14641 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    14701 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    14761 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    14821 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    14881 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    14941 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    15001 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    15061 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    15121 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    15181 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    15241 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    15301 ccattgggct gacgcaacaa gcaattgcgt aatcgtcgct gttgaagtca gtacgcatca
    15361 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    15421 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    15481 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    15541 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    15601 caaattccgg cgtacgcaag aagcgcacca tacggatctt cataatgaag tgatcgatca
    15661 gttcctgtgc ctgctgctca ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat
    15721 cgaggaacga cgccgtgcgc cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
    15781 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
    15841 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    15901 ccagctcctc acgcagacgg atggtggctt ccagatcttc gcctttttcc agacgggact
    15961 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    16021 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
    16081 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    16141 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    16201 cgtggaatga acttttaatc atattaatgc caccgaacgg atgcagcgcg cgctttaatg
    16261 gcgcatccgt ttgcagacca acaatttttt ccagcggctg gttaatatat cccgcatcat
    16321 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    16381 cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
    16441 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    16501 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
    16561 cgctggtatc aatatctatc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    16621 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
    16681 tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg gaaacaattc
    16741 gctctccaaa agagttagag cgattattca tttctgtatc aatcactacg cctaatacag
    16801 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    16861 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
    16921 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    16981 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    17041 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    17101 caaagtcgac atcgccactg cgggttccca tcatcaagcc ttccagcggc gtcattccca
    17161 ttgaagtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat
    17221 gcgccacaac caggccggag tcatcttccg ccagattcag cagagaatgt gcgcgctggg
    17281 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    17341 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    17401 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    17461 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    17521 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    17581 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    17641 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    17701 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    17761 cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    17821 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    17881 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    17941 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    18001 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    18061 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
    18121 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    18181 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    18241 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    18301 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaat
    18361 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    18421 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    18481 ttagaaacca cgaaggaaga gacgattggc gagaaattaa aggagaaaac catgatggaa
    18541 atccccagcc agacggtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    18601 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    18661 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    18721 aaccagatga cgaaagccat cagcagcagc aggaacagcg ccacaaagcc gcgattcagc
    18781 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    18841 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    18901 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    18961 agacatagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    19021 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    19081 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    19141 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    19201 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    19261 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    19321 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    19381 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    19441 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    19501 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    19561 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    19621 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    19681 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    19741 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    19801 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    19861 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    19921 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    19981 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    20041 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    20101 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    20161 tcaatagcaa tcggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    20221 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    20281 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    20341 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    20401 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    20461 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    20521 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    20581 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    20641 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    20701 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    20761 tttctgcact taatgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    20821 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    20881 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    20941 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    21001 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    21061 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    21121 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    21181 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    21241 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    21301 gaccactcac aaattaactt tcaatacttt ccagggtatc gtcattacaa ttaaatacct
    21361 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt gtcaaaaaaa
    21421 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
    21481 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
    21541 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
    21601 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
    21661 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
    21721 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
    21781 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct gatcgccgcg
    21841 ataaggtcag acaaagacaa caagggaaat tttcacagag cttttgatcg tcgtaggcca
    21901 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa
    21961 gaaccctgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
    22021 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
    22081 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgc
//
LOCUS       DAJTVE010000065        21994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000065 DAJTVE010000000
VERSION     DAJTVE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1461
                     /locus_tag="OO439_002328"
     CDS             73..1461
                     /locus_tag="OO439_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6316682.1"
                     /translation="MSAYLRYIVQKADSSFLYDNYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..2007
                     /gene="yeaK"
                     /locus_tag="OO439_002329"
     CDS             1504..2007
                     /gene="yeaK"
                     /locus_tag="OO439_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6316683.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2008..2112)
                     /locus_tag="OO439_002330"
     CDS             complement(2008..2112)
                     /locus_tag="OO439_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316684.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2282..2728
                     /locus_tag="OO439_002331"
     CDS             2282..2728
                     /locus_tag="OO439_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6316685.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO439_002332"
     CDS             complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO439_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6316686.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3603..4784
                     /gene="nimT"
                     /locus_tag="OO439_002333"
     CDS             3603..4784
                     /gene="nimT"
                     /locus_tag="OO439_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6316687.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4839..5186
                     /locus_tag="OO439_002334"
     CDS             4839..5186
                     /locus_tag="OO439_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6316688.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO439_002335"
     CDS             complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO439_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6316689.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5645..6670
                     /gene="dgcP"
                     /locus_tag="OO439_002336"
     CDS             5645..6670
                     /gene="dgcP"
                     /locus_tag="OO439_002336"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6316690.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6703..6801
                     /locus_tag="OO439_002337"
     CDS             6703..6801
                     /locus_tag="OO439_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6316691.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO439_002338"
     CDS             6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO439_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6316692.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6937..7185)
                     /locus_tag="OO439_002339"
     CDS             complement(6937..7185)
                     /locus_tag="OO439_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6316693.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7333..7515)
                     /locus_tag="OO439_002340"
     CDS             complement(7333..7515)
                     /locus_tag="OO439_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6316694.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO439_002341"
     CDS             complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO439_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6316695.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO439_002342"
     CDS             complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO439_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6316696.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO439_002343"
     CDS             complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO439_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6316697.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9842..10927
                     /gene="dmlA"
                     /locus_tag="OO439_002344"
     CDS             9842..10927
                     /gene="dmlA"
                     /locus_tag="OO439_002344"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6316698.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11178..12788
                     /gene="yeaV"
                     /locus_tag="OO439_002345"
     CDS             11178..12788
                     /gene="yeaV"
                     /locus_tag="OO439_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6316699.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12820..13944
                     /gene="yeaW"
                     /locus_tag="OO439_002346"
     CDS             12820..13944
                     /gene="yeaW"
                     /locus_tag="OO439_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6316700.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14000..14965
                     /gene="yeaX"
                     /locus_tag="OO439_002347"
     CDS             14000..14965
                     /gene="yeaX"
                     /locus_tag="OO439_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6316701.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO439_002348"
     CDS             complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO439_002348"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6316702.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO439_002349"
     CDS             complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO439_002349"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6316703.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO439_002350"
     CDS             complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO439_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6316704.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO439_002351"
     CDS             complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO439_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6316705.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19480..21390)
                     /locus_tag="OO439_002352"
     CDS             complement(19480..21390)
                     /locus_tag="OO439_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6316706.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21522..21866
                     /locus_tag="OO439_002353"
     CDS             21522..21866
                     /locus_tag="OO439_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6316707.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5373 a         5645 c         5367 g         5609 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata attatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa
     1981 ctgctcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
     2041 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
     2101 ttgatcgttc atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
     2161 aaatggcagt ttgatgacaa ttcgcctgat tttttatgca aaaaacgtaa agattttcta
     2221 cttccttcct gcagcaaacg taaagtaagc aggcttatta ttttttggca aggaaaccac
     2281 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
     2341 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
     2401 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
     2461 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt
     2521 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg
     2581 gcgcggcgtg accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac
     2641 tgttttaggc gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct
     2701 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
     2761 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
     2821 gcaactttct gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac
     2881 tgctgacgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg cgcaagattg
     2941 cgttcactca tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct
     3001 ttcgccatca tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc
     3061 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
     3121 ggctcagcac gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag
     3181 ggcgatattt tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac
     3241 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
     3301 caaaccgcat attgcggcgg caccatccat aaagcatttt ccaccgtaca ggtaattgca
     3361 ccatgtaatg ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
     3421 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
     3481 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
     3541 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
     3601 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
     3661 tgatgattgc cacaacatta cgcgtcacct ttatcggcgc agcaccgtta ctggatacga
     3721 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat
     3781 tggcctttgc gctaatctca cctttggcca ccccggtagc gcgacgtttt ggtatggaac
     3841 gtagcctgtt tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt
     3901 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
     3961 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
     4021 catattccct gacaatggga gctgcagcgg cactgggatc ggctatgata gtgccgctgg
     4081 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
     4141 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
     4201 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggta acattgtttc
     4261 ttgggatcaa ctcactgatc tattacgtga ttattggctg gcttccggcg atcctcatca
     4321 gtcacggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
     4381 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
     4441 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
     4501 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
     4561 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
     4621 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg
     4681 atgctaacgg tgactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg
     4741 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
     4801 gacgtgacaa atcatgcgtg aaagggagaa caaacactat gaatattcag tgcaaacgcg
     4861 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
     4921 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
     4981 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
     5041 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
     5101 aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
     5161 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
     5221 gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
     5281 caattgttgc tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg
     5341 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtac ttctggctga
     5401 gaagaggttg aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc
     5461 atttattgat ctcactcatt tatctacgct tagagtatcg cggtatcgtt ttgttttgca
     5521 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttggtga
     5581 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
     5641 tttcgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
     5701 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
     5761 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
     5821 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
     5881 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
     5941 tgcggcacgc aatcttggca tctccacatt tctgagcaca ccaattcact taccggatgg
     6001 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
     6061 ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact
     6121 ggttgaacag ttgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
     6181 aaccgggttg ccgaatccgc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc
     6241 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
     6301 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
     6361 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
     6421 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgca ttcagcagca
     6481 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
     6541 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
     6601 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc attcagcgcc
     6661 acattgctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
     6721 tttccggttg taatctttat tacagcggtc gtatttttag catggttttt tattggcggc
     6781 tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
     6841 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
     6901 aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat
     6961 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
     7021 gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac cgactatccc
     7081 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
     7141 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
     7201 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
     7261 gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac
     7321 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
     7381 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     7441 cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg tcacggtata
     7501 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     7561 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     7621 ttcaatgttg tgccactttt ctggagggaa cacctcaaac tgccccgctt cgataaggat
     7681 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     7741 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     7801 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     7861 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
     7921 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     7981 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     8041 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     8101 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     8161 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     8221 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     8281 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     8341 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     8401 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     8461 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     8521 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     8581 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     8641 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     8701 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     8761 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaata attaatcagg
     8821 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctatcg
     8881 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     8941 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     9001 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     9061 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     9121 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     9181 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     9241 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     9301 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     9361 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     9421 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     9481 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     9541 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     9601 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     9661 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcgttca
     9721 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     9781 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     9841 aatgatgaaa acgatgcgta ttgctgcgat cccgggagat gggattggca aagaagtcct
     9901 tcctgaaggg attcgcgtat tacaggctgc cgctgagcgc tggggcttcg acttgagttt
     9961 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    10021 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    10081 ggataccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    10141 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    10201 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    10261 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    10321 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    10381 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    10441 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
    10501 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    10561 ggtggcgtcc aatttgtttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    10621 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
    10681 gcctgtccac ggttccgcgc cggatatcta cgggaaaaat attgctaacc ctatcgccac
    10741 aatttgggcc ggtgcgatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    10801 agcgcataac ggtattctgg cagcgattga agaagtgatt gctcacgggc cgaaaacacc
    10861 ggatatgaaa ggcagtgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    10921 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
    10981 tttgcagtta ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    11041 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
    11101 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    11161 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    11221 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    11281 ccgacgcggc caacacaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
    11341 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
    11401 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    11461 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
    11521 aaacgcctgg attaaatatc gcaccacgtt cacaacaggc actcgaattt agcgtccctt
    11581 actccttctt ccactggggc atcagcacct gggcaactta tacgctggcc tcattaatca
    11641 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
    11701 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgtttctga
    11761 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    11821 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    11881 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
    11941 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggtc
    12001 caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg caaaacttcc
    12061 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    12121 ttttctactg gctgtggtgg atctcatacc cccctggcgt agcaatgttt gtcacccgcg
    12181 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    12241 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    12301 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
    12361 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    12421 tccttgcctc gcatatggat gcagtggcct acaccatggc ggcgaccagt acgcgtaatc
    12481 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    12541 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    12601 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    12661 ggctgaaaca ggattatgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    12721 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
    12781 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    12841 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
    12901 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    12961 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    13021 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    13081 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    13141 acctgcccat atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
    13201 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggag
    13261 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
    13321 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    13381 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    13441 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgc
    13501 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    13561 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    13621 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    13681 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    13741 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    13801 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    13861 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
    13921 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
    13981 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
    14041 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
    14101 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
    14161 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
    14221 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    14281 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    14341 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagtt
    14401 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    14461 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcgtc
    14521 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    14581 ttatacctgt ggccccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
    14641 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
    14701 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    14761 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    14821 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
    14881 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    14941 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
    15001 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    15061 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    15121 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    15181 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    15241 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    15301 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    15361 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
    15421 ctcttcacgc acgacaaagt tcaccgccag atcacgctca cgagccttgc gcagtcgcca
    15481 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    15541 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
    15601 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    15661 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
    15721 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    15781 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    15841 gtcaatcagg ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    15901 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    15961 gtcggtgatc ccgagaggat caattagcgc cagatgctca ccatcgaaaa gttgaatcaa
    16021 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatcctggg ctatcgccgg
    16081 aaagacacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    16141 attcaaattg tattctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    16201 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    16261 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    16321 cggtactttg tagcccgtga gctgacggca gcaaaaggtc accagtgact cttcggtaag
    16381 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    16441 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    16501 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
    16561 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
    16621 ctcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    16681 aagctcaccc ggttgacccg gtggtacttc attatcgtcg tcatccacca gtttggcttc
    16741 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    16801 gctgaccagc ggcgcacact cggtaagacc atagccttcc agcagatact gtcctgtcag
    16861 tttcacccaa cgctctgcca ccacttgctg cactggcatc ccaccgcctg cggaaagatg
    16921 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    16981 gttaacgcca gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    17041 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    17101 aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt ctttacctgg
    17161 atgcagcaac ggcccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    17221 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    17281 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    17341 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    17401 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    17461 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    17521 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    17581 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    17641 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    17701 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    17761 ctcccccata ttcacaaacg caggctgatc ggcgtagcgg acgaccgact gctcaaacat
    17821 atctatcaga gattgataac ggtcagggtt gatctccgtc ggaacatccg cgggataacg
    17881 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    17941 cccaagtgat aaacatgttg ttaacataac attaactcat cttaccagct tgataattag
    18001 acaacgaaaa ggttgcgaag cgcgtcacta tttattttta tagttgccgt atgaacgcag
    18061 aaacagcgga ccagccgctg tttctttttc tttaaaaaca acttgttact cggttacaat
    18121 tgtttcaact cttgcagggc caggattata ccaaccccat ccgccgtaac catagggcca
    18181 gccgcgtcca ccataaaacc atggatcaat tggctgcggc ggcataatca cctgttgagt
    18241 gatatgccag cgtttgtaac ctgttacctg cataaccata aatttatatg gtgcattatt
    18301 tactttgcca tccactgtgc cagtgattgg cccgacaacc gtcaccagtt gtccacgaaa
    18361 atcgaccgga tcaagaaaac cgttcacatc ggcataaatg cgcccgcgtg aaggttctcc
    18421 caaggttggt cttgctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    18481 ttgctggttt tgtaccacaa ccactttgcc gccaaagcgc gcctcctgac caacatataa
    18541 atctggcgca ttcatcacgc gtaccagatc ttgctgtggt gttggactgc tgcctttaat
    18601 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    18661 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    18721 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    18781 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    18841 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    18901 acggagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    18961 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    19021 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    19081 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccctgcgcca tcgtcattag
    19141 cgtagagaca ccaatcatcg gtaactccgc gccaagcgcc agcccttgcg caataccaat
    19201 accaatgcgc acgccagtaa agctaccggg gccgcgcccg taagccagag cgtttatgtc
    19261 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    19321 atgttcacga gggcaaagct caaaatgagc gttgacatta ccgtcgttcc acagagcgac
    19381 agagcagacc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    19441 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    19501 aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca gactggcgag
    19561 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaattacca gcacgccgcg
    19621 atcgtcggcg tcgcgaatca gtcgccctac tccctgcttg agagtaatga ccgcatccgg
    19681 cagctgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    19741 taacagtaga tcatccggcg aggtaaacgg caatttgtcg ataattacca atgacaatgt
    19801 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    19861 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    19921 aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcat gcgaggtaca
    19981 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgccagttg
    20041 ccgtgcacaa cccggttgat tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg
    20101 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    20161 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    20221 accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    20281 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    20341 ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg catccagcaa
    20401 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    20461 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    20521 acctggctca ccgagttgca gacgaaaatc ctgtgcactc tgggcaagac gatcagcgca
    20581 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    20641 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    20701 tagctggtgg gcttcgtcga agatcatgac gtccgcttca gggatcaact cgccaaatcc
    20761 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    20821 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    20881 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    20941 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    21001 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    21061 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    21121 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    21181 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    21241 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    21301 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    21361 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    21421 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    21481 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    21541 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    21601 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg gcacagattg
    21661 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    21721 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    21781 gtcatgcgcc agtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    21841 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    21901 tatcgataat actggtcggt tttacataaa tcgaccagag aataagatat tactgttgat
    21961 gcttctgcta attcattatt tatatttata attt
//
LOCUS       DAJTVE010000066        21739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000066 DAJTVE010000000
VERSION     DAJTVE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..978)
                     /gene="cmtA"
                     /locus_tag="OO439_002354"
     CDS             complement(<1..978)
                     /gene="cmtA"
                     /locus_tag="OO439_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6316708.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLV"
     gene            complement(1006..1449)
                     /gene="cmtB"
                     /locus_tag="OO439_002355"
     CDS             complement(1006..1449)
                     /gene="cmtB"
                     /locus_tag="OO439_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6316709.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1763..3754)
                     /gene="tkt"
                     /locus_tag="OO439_002356"
     CDS             complement(1763..3754)
                     /gene="tkt"
                     /locus_tag="OO439_002356"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6316710.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4032..4790
                     /gene="loiP"
                     /locus_tag="OO439_002357"
                     /gene_synonym="yggG"
     CDS             4032..4790
                     /gene="loiP"
                     /locus_tag="OO439_002357"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6316711.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4933..5853)
                     /gene="speB"
                     /locus_tag="OO439_002358"
     CDS             complement(4933..5853)
                     /gene="speB"
                     /locus_tag="OO439_002358"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6316712.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5991..7967)
                     /gene="speA"
                     /locus_tag="OO439_002359"
     CDS             complement(5991..7967)
                     /gene="speA"
                     /locus_tag="OO439_002359"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6316713.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7976..8107)
                     /gene="yqgB"
                     /locus_tag="OO439_002360"
     CDS             complement(7976..8107)
                     /gene="yqgB"
                     /locus_tag="OO439_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6316714.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8243..8458
                     /locus_tag="OO439_002361"
     CDS             8243..8458
                     /locus_tag="OO439_002361"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316715.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8762..9916
                     /gene="metK"
                     /locus_tag="OO439_002362"
     CDS             8762..9916
                     /gene="metK"
                     /locus_tag="OO439_002362"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6316716.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10352..11746
                     /gene="galP"
                     /locus_tag="OO439_002363"
     CDS             10352..11746
                     /gene="galP"
                     /locus_tag="OO439_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6316717.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11823..12320
                     /locus_tag="OO439_002364"
     CDS             11823..12320
                     /locus_tag="OO439_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6316718.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12415..13122
                     /gene="endA"
                     /locus_tag="OO439_002365"
     CDS             12415..13122
                     /gene="endA"
                     /locus_tag="OO439_002365"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6316719.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13202..13933
                     /gene="rsmE"
                     /locus_tag="OO439_002366"
     CDS             13202..13933
                     /gene="rsmE"
                     /locus_tag="OO439_002366"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6316720.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13946..14896
                     /gene="gshB"
                     /locus_tag="OO439_002367"
     CDS             13946..14896
                     /gene="gshB"
                     /locus_tag="OO439_002367"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6316721.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15005..15568
                     /locus_tag="OO439_002368"
     CDS             15005..15568
                     /locus_tag="OO439_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6316722.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15568..15984
                     /gene="ruvX"
                     /locus_tag="OO439_002369"
     CDS             15568..15984
                     /gene="ruvX"
                     /locus_tag="OO439_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6316723.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16159..17139)
                     /locus_tag="OO439_002370"
     CDS             complement(16159..17139)
                     /locus_tag="OO439_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6316724.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17157..17861
                     /gene="yggS"
                     /locus_tag="OO439_002371"
     CDS             17157..17861
                     /gene="yggS"
                     /locus_tag="OO439_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6316725.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17879..18445
                     /gene="yggT"
                     /locus_tag="OO439_002372"
     CDS             17879..18445
                     /gene="yggT"
                     /locus_tag="OO439_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6316726.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18442..18732
                     /locus_tag="OO439_002373"
     CDS             18442..18732
                     /locus_tag="OO439_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6316727.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18740..19333
                     /locus_tag="OO439_002374"
     CDS             18740..19333
                     /locus_tag="OO439_002374"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6316728.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19326..20462
                     /gene="hemW"
                     /locus_tag="OO439_002375"
     CDS             19326..20462
                     /gene="hemW"
                     /locus_tag="OO439_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6316729.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20773..21739
                     /locus_tag="OO439_002376"
                     /pseudo
     CDS             20773..21739
                     /locus_tag="OO439_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         5385 a         5655 c         5533 g         5166 t
ORIGIN      
        1 caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt tcggcgtcag
       61 tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac cgtccagtaa
      121 gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg tcagcggctt
      181 catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa taatcatcgc
      241 cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca gcagcagccc
      301 taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt taaccgaagc
      361 ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga ggaaaagcac
      421 tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta ccagcgcctc
      481 aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa cttcaaaacc
      541 cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa ccatctcaaa
      601 accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca gcccaccgag
      661 cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga tcacaccaat
      721 agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac caccggtaga
      781 accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg cgaaatgttc
      841 gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg caataaaagc
      901 accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga cctttgcacg
      961 agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc cgcggctgat
     1021 tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta tttcttcttc
     1081 gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat cggcagcaga
     1141 aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac cttgttcaag
     1201 taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat gaggcattgc
     1261 cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt taattgcctg
     1321 aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga aatcgatagc
     1381 ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa aatagtcact
     1441 aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta aaaataaaat
     1501 actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa aacagtgaat
     1561 tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa tacgcatatc
     1621 gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc tacaacacgc
     1681 tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt cgccgaagcg
     1741 acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg acgttgtcga
     1801 cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg aaggtggtca
     1861 taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca atacccgctt
     1921 ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa gccgcatcct
     1981 gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg ccttcggcag
     2041 tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg atgaagatca
     2101 gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg tttgccagtt
     2161 gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc gcggtcggac
     2221 cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg tcacacggac
     2281 gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc ggctggtgag
     2341 tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc tgacgctgtt
     2401 tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc aggaaggtgg
     2461 aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg gtcataccga
     2521 actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt gctttagaac
     2581 cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg aattccggca
     2641 acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg attttcgccg
     2701 gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca gacggcattt
     2761 cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc gcgtaagccg
     2821 caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca tcccactgcg
     2881 catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt tcgcgggtca
     2941 gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg
     3001 gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca gtcactgcgc
     3061 gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg tcgcgaataa
     3121 cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag ccttcaacgt
     3181 gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg
     3241 tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg tcgcccatga
     3301 aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc gccgccagcg
     3361 ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg
     3421 tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta gagtgcagct
     3481 gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt
     3541 agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca gcccaggacg
     3601 gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg tcagccatac
     3661 ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac
     3721 gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg atgatgaagg
     3781 gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca
     3841 atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc agaaatctct
     3901 ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt ccttatactg
     3961 agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg accttgtgcg
     4021 gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca acggtactga
     4081 ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa gcttttcagg
     4141 cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt caggagatgg
     4201 acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg acaactattg
     4261 ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg tatatggcga
     4321 aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc gggctgatgg
     4381 atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt
     4441 taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg
     4501 cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt gatctgggcg
     4561 agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat gattattctt
     4621 acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac
     4681 tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg gcatccgccg
     4741 aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa agcctggtgg
     4801 tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg cacatttgtt
     4861 accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta cgggtggcat
     4921 cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct ccagcgccag
     4981 cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca cttccactac
     5041 gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag cgcgatcgga
     5101 ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat ccaggcagtc
     5161 gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt gggcgataac
     5221 gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc cgttgtcttt
     5281 atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca gaccttcttt
     5341 cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt
     5401 gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag
     5461 cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg cagccagcag
     5521 cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa aggcatatac
     5581 cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg
     5641 gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac cgtggcgacc
     5701 acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc agtctgcatc
     5761 gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat tggaaaccag
     5821 tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata
     5881 taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg tcacaataaa
     5941 ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac ttactcatct
     6001 tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt
     6061 tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag cgttttcgga
     6121 tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga cagttctact
     6181 tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc gaacaggttg
     6241 tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag catcggcgga
     6301 ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcaatatag
     6361 tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg acgttccggc
     6421 acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc ccatgcgtcc
     6481 ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg ttcctgcagt
     6541 tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg
     6601 cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa
     6661 gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca ttcacgcaaa
     6721 gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct ttgcagcgcg
     6781 cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc cacgccgatg
     6841 atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc ggtgattacc
     6901 gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat gatgttgttg
     6961 gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt gccttcatag
     7021 tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag cttgtgcagt
     7081 tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat attcgccatc
     7141 tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc ttcacgcagg
     7201 gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt ttccccgccg
     7261 gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga
     7321 acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat cttctcaatg
     7381 accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat atattcgcgg
     7441 tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc cagtactgcc
     7501 atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg aatcagggac
     7561 tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta atcgccgtta
     7621 tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc
     7681 aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc acgagttttc
     7741 actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctgatg
     7801 tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc
     7861 agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg
     7921 tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa
     7981 ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc gataacaacc
     8041 cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca accggtttct
     8101 ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg
     8161 agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc
     8221 ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc
     8281 agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc agacaacggt
     8341 tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct
     8401 gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc
     8461 cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat
     8521 gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa
     8581 aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc
     8641 ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc
     8701 taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa
     8761 tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc
     8821 tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt
     8881 tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag
     8941 cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca
     9001 ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc
     9061 tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca
     9121 gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag cacctatcac
     9181 ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg
     9241 gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg
     9301 tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga aatcgctgca
     9361 agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc tgacttctgc
     9421 caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga tgggtgactg
     9481 cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac gtcacggtgg
     9541 cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct acgcagcacg
     9601 ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc
     9661 ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg gtactgagaa
     9721 agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc gcccatacgg
     9781 tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca
     9841 ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc
     9901 tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc gctggccttt
     9961 ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa agctgcccgc
    10021 acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact actgaaaccg
    10081 attacaccaa taacaccagc caaatatttg taatattttc atattattat tcagttttca
    10141 cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt taatctatga
    10201 tgatataact caattatttt catgcactta aaccataact aagataaatg ttagtgtaag
    10261 cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct atcttaattc
    10321 acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac aggggcggtc
    10381 aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat tactctttgg
    10441 cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat tccagattac
    10501 ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt
    10561 gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat
    10621 tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag tactgattct
    10681 ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac cgctgtacct
    10741 ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc agttgatgat
    10801 cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca ccggtgcatg
    10861 gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg gtgtcttctt
    10921 cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt
    10981 gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg aaatccgtga
    11041 aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca acttccgccg
    11101 cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga tgaacgtcat
    11161 catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca cagagcaaat
    11221 gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg caatcggcct
    11281 tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga tggctgctgg
    11341 catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg cgcagtattt
    11401 cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg gtccgctgat
    11461 ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca tcacctgctc
    11521 cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga ccatgctcaa
    11581 cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac tgtttatcct
    11641 gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa
    11701 cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc
    11761 ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta tcctctgccg
    11821 ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc ggcctgcggg
    11881 aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca aaactctctt
    11941 acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa atccgcctca
    12001 atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta ccgcacgaac
    12061 tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc aaagagtgga
    12121 agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag ttcgaactgc
    12181 aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat cagcttaccg
    12241 tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt cactgcggtg
    12301 aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc tggctgattg
    12361 cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta agtgatgtac
    12421 cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc ggcgttggcc
    12481 gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca cgctgacgcg
    12541 cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat
    12601 ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg cgtagagtgg
    12661 gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca ggacggtgga
    12721 cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat gtataacctg
    12781 cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag ccagtggaat
    12841 ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc
    12901 gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat gcgcgaccaa
    12961 tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa caagatgtat
    13021 ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca aggcaatcat
    13081 aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact agcgggattc
    13141 tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac
    13201 tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc acatcgcgct
    13261 ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt
    13321 gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg ccagcaaaaa
    13381 aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc cgctgcatat
    13441 tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc agaaatcgat
    13501 cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg ttaaactgga
    13561 tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg ctgcctgtga
    13621 gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg aagcctggtg
    13681 tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta acagcatcaa
    13741 tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag gcggtttatc
    13801 ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt tgggacctcg
    13861 cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag tacgatttgg
    13921 cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg accccatcgc
    13981 aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac agcgtcgtgg
    14041 ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg aagcccgcgc
    14101 ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga
    14161 acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc cgccgtttga
    14221 taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag ggacgctgat
    14281 cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg cctggttctc
    14341 tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag cgttctggga
    14401 gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt cgattttccg
    14461 cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg agaatggcac
    14521 tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca aacgcgtgct
    14581 ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg ggggcgaaac
    14641 ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg
    14701 gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt ttgttggtct
    14761 ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct gtattcgtga
    14821 gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca tcgaagcacg
    14881 tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct gagtgacagc
    14941 gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa cagaacctct
    15001 gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg atccgatttt
    15061 ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt
    15121 caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga agatcacgcc
    15181 ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg gtccgctggc
    15241 tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca gcattcgcat
    15301 ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa
    15361 acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag gtcaactgga
    15421 acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata ttctgttcaa
    15481 aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg atattctcac
    15541 catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt cgacttcggc
    15601 accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg ccctttgcct
    15661 gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt actgaaagag
    15721 tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac cgagcaacca
    15781 ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg tgttgaagta
    15841 aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt tgaacagggc
    15901 ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat tattctcgaa
    15961 agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt cgttaccgcc
    16021 atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga ttgtaggtct
    16081 gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg ataagcttgc
    16141 acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa aactctgctg
    16201 aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact ggtgggtttt
    16261 cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa acagcgccac
    16321 gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta aactacctgc
    16381 cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca gtcgctcaac
    16441 tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg tttctgccgc
    16501 cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa tcacatcagg
    16561 atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca aaccaatttc
    16621 ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat cttccagcgt
    16681 cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca gcgtggtaga
    16741 tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct tgagtaattc
    16801 cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag gtaacagccg
    16861 taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc gctggttttc
    16921 cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc gctgatcgtc
    16981 atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg cagcttccat
    17041 tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt
    17101 atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct cggaaaatga
    17161 acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt
    17221 gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg
    17281 ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac gttcaggaag
    17341 gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa tggcatttta
    17401 ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac tggtgtcata
    17461 ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca gaacttcccc
    17521 ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc gggattcaac
    17581 tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg
    17641 ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc
    17701 aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac acgctctctc
    17761 tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg
    17821 gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa ggaacgccat
    17881 gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg tgctgttatt
    17941 acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac agtttgtagt
    18001 gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa tgggccccat
    18061 tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca tcgtgctgtt
    18121 taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc tgctgaaaac
    18181 catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct gggtaagcca
    18241 gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc tgcgcccgat
    18301 tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc tcgttctgct
    18361 gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa acatgctgct
    18421 gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg tctggtttta
    18481 cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca tggcgacgaa
    18541 gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca tctggtgaag
    18601 tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt
    18661 ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg
    18721 ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt
    18781 gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc aaacagacct
    18841 cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga ttctgaaagc
    18901 gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg gtctggcggt
    18961 agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg aagacgcgac
    19021 cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg acgaccaacg
    19081 tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc cgactccgct
    19141 ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt
    19201 tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg aactgacccg
    19261 cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt
    19321 acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg tgcgtgcaga
    19381 aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg cacgacgatt
    19441 atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag ggccgtgaag
    19501 taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa
    19561 cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa attactatgg
    19621 aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt gctggtgtga
    19681 accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga cttgggcgta
    19741 ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg gggctgcgta
    19801 gctttaacct cgatttgatg catggactac cggatcaatc actggaagag gcgcttggcg
    19861 atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg
    19921 aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac gcgctgtggg
    19981 atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa tatgaaactt
    20041 ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg cgatttggtg
    20101 actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat gggcgcattc
    20161 tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat ctggaaagcc
    20221 agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc ttccgtctgc
    20281 tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa gatgtgattc
    20341 gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg gattactggc
    20401 agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc ctcgctgagt
    20461 aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta attaacttcg
    20521 gcaaaatata tttcttactg aataaacatc gatgatattt attagatata tctagtgaat
    20581 atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat ttaatattta
    20641 atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata ggttaaccaa
    20701 tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca tccttcaaca
    20761 gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc atccttgctg
    20821 gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt agcacacaac
    20881 ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga ggtaaaacag
    20941 ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg taatgcgcga
    21001 taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc cgccagtgat
    21061 ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt taaagatcag
    21121 gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga ctcaacaaaa
    21181 gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt ctatgcgaaa
    21241 aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca gccaagtccg
    21301 accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc atttggcgaa
    21361 gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt gccttcctgg
    21421 gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca cgcctcaatt
    21481 cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga acagaaacaa
    21541 atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg ggaaaaaatt
    21601 gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa agtggataaa
    21661 gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga tccaaaacaa
    21721 gctgctttgt tagagaagt
//
LOCUS       DAJTVE010000067        21501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000067 DAJTVE010000000
VERSION     DAJTVE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1115)
                     /gene="fsr"
                     /locus_tag="OO439_002377"
     CDS             complement(<1..1115)
                     /gene="fsr"
                     /locus_tag="OO439_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6316730.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGL"
     gene            1333..2985
                     /gene="ushA"
                     /locus_tag="OO439_002378"
     CDS             1333..2985
                     /gene="ushA"
                     /locus_tag="OO439_002378"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6316731.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3022..3501)
                     /gene="ybaK"
                     /locus_tag="OO439_002379"
     CDS             complement(3022..3501)
                     /gene="ybaK"
                     /locus_tag="OO439_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6316732.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3705..4499)
                     /locus_tag="OO439_002380"
     CDS             complement(3705..4499)
                     /locus_tag="OO439_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6316733.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4637..4978
                     /locus_tag="OO439_002381"
     CDS             4637..4978
                     /locus_tag="OO439_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6316734.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5193..7697)
                     /gene="copA"
                     /locus_tag="OO439_002382"
     CDS             complement(5193..7697)
                     /gene="copA"
                     /locus_tag="OO439_002382"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6316735.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7959..8891
                     /gene="glsA"
                     /locus_tag="OO439_002383"
     CDS             7959..8891
                     /gene="glsA"
                     /locus_tag="OO439_002383"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6316736.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8894..10186
                     /locus_tag="OO439_002384"
     CDS             8894..10186
                     /locus_tag="OO439_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6316737.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10311..10718
                     /gene="cueR"
                     /locus_tag="OO439_002385"
     CDS             10311..10718
                     /gene="cueR"
                     /locus_tag="OO439_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6316738.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10719..11177)
                     /locus_tag="OO439_002386"
     CDS             complement(10719..11177)
                     /locus_tag="OO439_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6316739.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11174..12091)
                     /locus_tag="OO439_002387"
     CDS             complement(11174..12091)
                     /locus_tag="OO439_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6316740.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12237..12914
                     /gene="fetA"
                     /locus_tag="OO439_002388"
     CDS             12237..12914
                     /gene="fetA"
                     /locus_tag="OO439_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6316741.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12901..13680
                     /gene="fetB"
                     /locus_tag="OO439_002389"
     CDS             12901..13680
                     /gene="fetB"
                     /locus_tag="OO439_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6316742.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13743..14597)
                     /gene="cnoX"
                     /locus_tag="OO439_002390"
     CDS             complement(13743..14597)
                     /gene="cnoX"
                     /locus_tag="OO439_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6316743.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14658..15467)
                     /gene="ybbO"
                     /locus_tag="OO439_002391"
     CDS             complement(14658..15467)
                     /gene="ybbO"
                     /locus_tag="OO439_002391"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6316744.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15457..16080)
                     /gene="tesA"
                     /locus_tag="OO439_002392"
     CDS             complement(15457..16080)
                     /gene="tesA"
                     /locus_tag="OO439_002392"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6316745.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16051..16737
                     /locus_tag="OO439_002393"
     CDS             16051..16737
                     /locus_tag="OO439_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6316746.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16734..19148
                     /locus_tag="OO439_002394"
     CDS             16734..19148
                     /locus_tag="OO439_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6316747.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19382..20476)
                     /gene="mnmH"
                     /locus_tag="OO439_002395"
     CDS             complement(19382..20476)
                     /gene="mnmH"
                     /locus_tag="OO439_002395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6316748.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20545..21471)
                     /gene="allS"
                     /locus_tag="OO439_002396"
                     /pseudo
     CDS             complement(20545..21471)
                     /gene="allS"
                     /locus_tag="OO439_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         4980 a         5663 c         5775 g         5083 t
ORIGIN      
        1 agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa aaagagtcca
       61 gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat ggcagagaat
      121 gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg caggctggcg
      181 tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc
      241 cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg
      301 aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg catcagataa
      361 aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac
      421 agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg
      481 ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc
      541 acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg
      601 ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag
      661 atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc
      721 ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac tgcgccaaaa
      781 ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac
      841 gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag
      901 gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac
      961 tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag
     1021 aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg
     1081 cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt tcagggttgc
     1141 gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt atgcgtcgtc
     1201 tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc
     1261 ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg aaatcaggtc
     1321 agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt
     1381 acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt
     1441 ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg
     1501 gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc
     1561 gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat
     1621 gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat
     1681 catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg
     1741 ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa accgtgggcg
     1801 ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga tgacacagca
     1861 aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc cgatgaagcg
     1921 aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc
     1981 catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga tgtggagatg
     2041 gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagatccg
     2101 gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa
     2161 ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga
     2221 cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg
     2281 gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt gctgtacacc
     2341 cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc
     2401 aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac
     2461 aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc
     2521 actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc
     2581 gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc
     2641 gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat
     2701 tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg caaactcaac
     2761 gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta
     2821 aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg
     2881 aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg
     2941 gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga
     3001 tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag
     3061 gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac
     3121 ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg
     3181 ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc
     3241 ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc
     3301 tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag
     3361 cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt
     3421 ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag
     3481 taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca
     3541 tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt
     3601 gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa
     3661 tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc
     3721 ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc
     3781 ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac
     3841 agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc
     3901 caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg
     3961 tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc
     4021 ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg
     4081 ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg
     4141 atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg
     4201 tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc
     4261 aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg
     4321 tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga
     4381 atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag
     4441 gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata
     4501 cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg
     4561 gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa
     4621 gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct
     4681 tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca
     4741 tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca ccactgagat
     4801 ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa acctccaggc
     4861 ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat
     4921 tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg
     4981 ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga
     5041 gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac
     5101 cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc
     5161 cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac
     5221 cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc
     5281 gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg aataccgatg
     5341 ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt tgcgcgggaa
     5401 atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat cgccgcggtt
     5461 tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg ggccagcgct
     5521 ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt
     5581 ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc aattccagct
     5641 tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat
     5701 cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca
     5761 gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat
     5821 gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc atttaacagc
     5881 gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc cagcccgcgc
     5941 aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc gaggatcgcc
     6001 cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa tgcctgcgct
     6061 tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc ggtcagcgtc
     6121 ccggttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc
     6181 cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga catcggcgtc
     6241 gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat caccagggta
     6301 taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac cagcgcaatc
     6361 accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc gatttctggc
     6421 ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt atggctgcca
     6481 accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc atggacgctt
     6541 tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc atccagccat
     6601 gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt
     6661 aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc ggtgaccact
     6721 cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgt
     6781 gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca
     6841 tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa
     6901 tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc tgcaccgttc
     6961 agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat cactgccagg
     7021 gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat catgttatcg
     7081 ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat tgcctgccag
     7141 cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg tttagcgtca
     7201 tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa atcttgtggg
     7261 gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg
     7321 cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg
     7381 ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa
     7441 accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg
     7501 cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca
     7561 gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc cgggcgctgt
     7621 tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg
     7681 tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt
     7741 taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta
     7801 aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg
     7861 tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg
     7921 aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac
     7981 agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg
     8041 attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct atcgtgacct
     8101 gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct
     8161 ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca
     8221 aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg
     8281 gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta
     8341 acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag caactggctg
     8401 gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata
     8461 accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag
     8521 cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa ctggcaacgc
     8581 ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc gttcttcagg
     8641 ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg
     8701 gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg
     8761 cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggca
     8821 acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt
     8881 ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg
     8941 gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg
     9001 gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt
     9061 ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg
     9121 tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag cgctctcgtt
     9181 gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt ttggcgctta
     9241 tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt
     9301 ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg tagggcggct
     9361 ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt
     9421 ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt
     9481 ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc
     9541 ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt
     9601 taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat cggcattaga
     9661 gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc tcgggcatgt
     9721 gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa
     9781 cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct
     9841 aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg tggtgttgat
     9901 tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca gcgccacctt
     9961 tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc
    10021 ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat
    10081 ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact
    10141 cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg
    10201 cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt
    10261 gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca
    10321 gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat gaagagaagg
    10381 ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc
    10441 tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg
    10501 gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca
    10561 cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc
    10621 agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg
    10681 aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata
    10741 tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg
    10801 ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc
    10861 ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta
    10921 tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg
    10981 agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag
    11041 accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc
    11101 agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg
    11161 accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcggcaa
    11221 tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt
    11281 tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca
    11341 ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg
    11401 cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg
    11461 ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc
    11521 cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg
    11581 aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc
    11641 cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct
    11701 gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca
    11761 ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc gcgtcaatca
    11821 cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct
    11881 gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa
    11941 acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta
    12001 ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga
    12061 caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata
    12121 atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc
    12181 cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc
    12241 aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga
    12301 ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt
    12361 ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg
    12421 gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac
    12481 aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga
    12541 tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg
    12601 aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg
    12661 aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg
    12721 atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc
    12781 gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta
    12841 atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct
    12901 atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct ggtggtggtg
    12961 gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct ctggagcgtc
    13021 ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg
    13081 gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac
    13141 gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg
    13201 gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg
    13261 atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg
    13321 tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg
    13381 agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg
    13441 gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg
    13501 atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg
    13561 gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc
    13621 tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga
    13681 tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa
    13741 tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta
    13801 cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga
    13861 tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc
    13921 agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt
    13981 tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc
    14041 atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac cgcttctgca
    14101 tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat ctccccgttc
    14161 tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa attgccttcc
    14221 tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc
    14281 agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg
    14341 ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc
    14401 tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcaaggctt
    14461 tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc
    14521 accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca
    14581 atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct
    14641 taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc cggcagcagg
    14701 cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat cttcggcttc
    14761 tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg
    14821 gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg
    14881 aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc
    14941 tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc gtaagcgcca
    15001 cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg
    15061 tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg
    15121 gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata catgccgaat
    15181 ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac ttcgtcggct
    15241 gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa tcccatgctg
    15301 ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa accctggcgt
    15361 tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat
    15421 ttttgcataa ctttacctgt caggatctct gttgctttat gagtcatgat ttactaaagg
    15481 ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat
    15541 accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag
    15601 cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg
    15661 gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt
    15721 gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa
    15781 accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt tcagcagagc
    15841 cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga
    15901 cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg
    15961 atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa
    16021 ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat
    16081 catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag
    16141 ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca
    16201 accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg
    16261 ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc
    16321 ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag
    16381 cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg
    16441 ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt
    16501 gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc
    16561 gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt
    16621 tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg
    16681 gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg
    16741 cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa
    16801 gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct
    16861 taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag
    16921 tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga
    16981 ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg
    17041 atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg
    17101 cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca
    17161 ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt
    17221 ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata
    17281 aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg
    17341 agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc gaacaacgct
    17401 ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt
    17461 tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga
    17521 tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag
    17581 ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag
    17641 ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc
    17701 ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca
    17761 ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc
    17821 agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc
    17881 tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc
    17941 tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct
    18001 ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg cgcctggcgg
    18061 ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct
    18121 cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac cgctggcagc
    18181 agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca gaacaggtag
    18241 caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt tatccggtag
    18301 tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa gatgaggcgc
    18361 ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat ccgattgtcg
    18421 ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg gcgaaacgct
    18481 taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag ttccgcgcta
    18541 aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc tattttattt
    18601 tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc cgctgggaga
    18661 atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc ctgttagata
    18721 ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg gcgctggagg
    18781 tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag gtgcaggtgg
    18841 gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg gggaaaaaac
    18901 tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct ggcctggtag
    18961 ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt gatttcccgt
    19021 gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg ctgctgtcgc
    19081 ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc aggcagtttg
    19141 cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc tacgaagagc
    19201 gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac gccgcatctg
    19261 gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc ggttttttat
    19321 atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag aataccgccc
    19381 atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag acaacttttt
    19441 ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta agtaacggca
    19501 ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc agcgccgtat
    19561 ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta atagccgaaa
    19621 gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg tacgcgtggg
    19681 taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga cggatctcaa
    19741 acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg cattccggca
    19801 ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc aaatcctgac
    19861 gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc tggctaagtt
    19921 gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct tccagatcaa
    19981 caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa ccgccaatca
    20041 gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg cgcagtgcct
    20101 tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag ctttgcacaa
    20161 tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt tgcaggcacg
    20221 ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt ttatgcccca
    20281 gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg gcgcgttcat
    20341 cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca aactcgatag
    20401 gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag tcctgttccg
    20461 tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct attttacgcg
    20521 ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt ctaaaaatcc
    20581 gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg ctttgccgct
    20641 gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg ggattacccg
    20701 gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca aaaaaccaat
    20761 gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa taatctcttt
    20821 ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt cttcaatatt
    20881 gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt tcgccagcgg
    20941 atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa gactaaaggt
    21001 atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt cgtacaatag
    21061 cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa aggggtaacg
    21121 ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca ggttgttgat
    21181 gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc tgggcatact
    21241 ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag ccgctgtcaa
    21301 cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt ccagaagttt
    21361 aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt ttgaaaaact
    21421 tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca tcttcaggta
    21481 tcccctttta aatccgcaag t
//
LOCUS       DAJTVE010000068        21173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000068 DAJTVE010000000
VERSION     DAJTVE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1366
                     /gene="espL1"
                     /locus_tag="OO439_002397"
                     /pseudo
     CDS             1..1366
                     /gene="espL1"
                     /locus_tag="OO439_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1538..1645
                     /locus_tag="OO439_002398"
     CDS             1538..1645
                     /locus_tag="OO439_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316749.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1698..2456)
                     /locus_tag="OO439_002399"
     CDS             complement(1698..2456)
                     /locus_tag="OO439_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6316750.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2743..3672
                     /gene="pfkB"
                     /locus_tag="OO439_002400"
     CDS             2743..3672
                     /gene="pfkB"
                     /locus_tag="OO439_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6316751.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3773..4063
                     /gene="ghoS"
                     /locus_tag="OO439_002401"
     CDS             3773..4063
                     /gene="ghoS"
                     /locus_tag="OO439_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6316752.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4169..5029
                     /locus_tag="OO439_002402"
     CDS             4169..5029
                     /locus_tag="OO439_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6316753.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5070..5606)
                     /locus_tag="OO439_002403"
     CDS             complement(5070..5606)
                     /locus_tag="OO439_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6316754.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5753..6421
                     /gene="hxpB"
                     /locus_tag="OO439_002404"
     CDS             5753..6421
                     /gene="hxpB"
                     /locus_tag="OO439_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6316755.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6584..7174
                     /locus_tag="OO439_002405"
     CDS             6584..7174
                     /locus_tag="OO439_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6316756.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7307..8698
                     /gene="tcyP"
                     /locus_tag="OO439_002406"
     CDS             7307..8698
                     /gene="tcyP"
                     /locus_tag="OO439_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6316757.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8702..9518)
                     /locus_tag="OO439_002407"
                     /pseudo
     CDS             complement(8702..9518)
                     /locus_tag="OO439_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9795..10058)
                     /gene="cedA"
                     /locus_tag="OO439_002408"
     CDS             complement(9795..10058)
                     /gene="cedA"
                     /locus_tag="OO439_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6316758.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10241..12502
                     /gene="katE"
                     /locus_tag="OO439_002409"
     CDS             10241..12502
                     /gene="katE"
                     /locus_tag="OO439_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6316759.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12760..13518)
                     /gene="chbG"
                     /locus_tag="OO439_002410"
     CDS             complement(12760..13518)
                     /gene="chbG"
                     /locus_tag="OO439_002410"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6316760.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13531..14883)
                     /gene="celF"
                     /locus_tag="OO439_002411"
                     /gene_synonym="chbF"
     CDS             complement(13531..14883)
                     /gene="celF"
                     /locus_tag="OO439_002411"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6316761.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14988..15830)
                     /gene="chbR"
                     /locus_tag="OO439_002412"
     CDS             complement(14988..15830)
                     /gene="chbR"
                     /locus_tag="OO439_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6316762.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15838..16188)
                     /gene="chbA"
                     /locus_tag="OO439_002413"
     CDS             complement(15838..16188)
                     /gene="chbA"
                     /locus_tag="OO439_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6316763.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16239..17597)
                     /gene="chbC"
                     /locus_tag="OO439_002414"
     CDS             complement(16239..17597)
                     /gene="chbC"
                     /locus_tag="OO439_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6316764.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17682..18002)
                     /gene="chbB"
                     /locus_tag="OO439_002415"
     CDS             complement(17682..18002)
                     /gene="chbB"
                     /locus_tag="OO439_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6316765.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18301..18639)
                     /gene="osmE"
                     /locus_tag="OO439_002416"
     CDS             complement(18301..18639)
                     /gene="osmE"
                     /locus_tag="OO439_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6316766.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18841..19668
                     /gene="nadE"
                     /locus_tag="OO439_002417"
     CDS             18841..19668
                     /gene="nadE"
                     /locus_tag="OO439_002417"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6316767.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19898..20785
                     /gene="cho"
                     /locus_tag="OO439_002418"
     CDS             19898..20785
                     /gene="cho"
                     /locus_tag="OO439_002418"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6316768.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20745..>21173)
                     /gene="ves"
                     /locus_tag="OO439_002419"
     CDS             complement(20745..>21173)
                     /gene="ves"
                     /locus_tag="OO439_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6316769.1"
                     /translation="SLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVV
                     KAKLTEGQMSMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLT
                     TDQGACWFDGRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5646 a         5224 c         4850 g         5453 t
ORIGIN      
        1 gcccttgatt cattgagaaa tggaaaaggc aaagtcaacc tgattaaaca ctactcctcc
       61 gttgaatcca tacagcagca tgtcccctta gtccgggacg cggagttcag agcattactt
      121 cgccatcctc ctgcagggag tcgcgttatc gcgagtaagg attttggctt cgctttagat
      181 attttctttt gtcgaatgat ggcaaacaat gtcagtcata tgtccgcgat tttatatata
      241 gacaatcata ctttgtcagt aaggctacga ataaagcagt cagtgtatgg acaattaaat
      301 tatgttgtgt ctgtttacga cccgaacgat accaacgttg ccgtcagagg cacccacagg
      361 gcagcacggg gctttctctc gcttgataag ttcatcagtt cagctcccga tgctcagacc
      421 tgggctgata ggtatgttcg caactgtgca attgctattc ttcctctatt acctgaggga
      481 gttccagttg ctattttagc gggtatcacg acacgaatgc catttgcccc tatacatcca
      541 tcggcaatgt tgttaataat ggccacaggc cagactcaat agcttattac actattcaaa
      601 cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca gaatagcgtt
      661 ggtacacctg ctttatttct ggctatgatg aacggacaaa ctgacaacgt aaaaatattt
      721 atgcaagaaa ttcagtcact ggtatacaat catatcattc atgaagataa tctggttaaa
      781 ttactgcaaa ctaaaagtgc taacgaaaca cctggactct atatctccat gttgtatgga
      841 ttcgatgaaa taatcgatat ctttctgaat gcattaacca ctccaatagc acaagaactt
      901 ttaaacaaaa aaaatggtga tgagtatttt agccatgaaa atacatgatg gtgagccagg
      961 attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc tctctaaaat
     1021 taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt tattaaaagg
     1081 agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta atgaagacgt
     1141 cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt ttagtcaaca
     1201 tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg ttcatatagc
     1261 cattcatcat aagcattata aaactgtaga aacatattat gctgctataa atgtcatcag
     1321 ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag ctatttacga
     1381 tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga ccatgtgcac
     1441 aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctatccct tttgttacac
     1501 tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac gctttgataa
     1561 aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt tgttgctacg
     1621 ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga ccgcataaca
     1681 cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg acgatcggta
     1741 tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc caccttcagt
     1801 ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt tgtatcttcc
     1861 gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg ccatgcataa
     1921 gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata tttgtcgtaa
     1981 cgcacacctg ggccgaattc aaagcggaaa ctgtgtaccg ggccattcaa aaactggcga
     2041 ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc tgtcagccag
     2101 cttgcctgac caaataaata gtcataatca gttaaattaa aacggctacg cccgcccgcc
     2161 gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt cccccacagc
     2221 gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag tgaggagctt
     2281 ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa aggtttttta
     2341 gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc attcagtgac
     2401 gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag cttcatttat
     2461 caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc ttaaggatca
     2521 aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac ttagcaatgt
     2581 attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt tatatatatt
     2641 tatctgcaaa attttaaata aagctccaat aaatcatatt gttaatttct tcactttccg
     2701 ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg tatctatacg
     2761 ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta tcccgaagga
     2821 aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa cgtcgcccgc
     2881 gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg cgcgaccggc
     2941 gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt agaagccaaa
     3001 gactggaccc ggcagaattt acatgtacat gtggaagcaa gcggtgagca gtatcgtttt
     3061 gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga gcaagttctg
     3121 gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg tgtgaagctg
     3181 gaaaaattaa cccaactgat ttccgcttcg caaaaacaag ggatccgctg catcgtcgac
     3241 agttctggcg aagcgttaag tgcagcactg gcaattggta acatcgagtt ggttaagcct
     3301 aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccgga cgatgtccgc
     3361 aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt ttcccttggt
     3421 ccacaaggag cgctgggtgt tgatagtgaa aactgcattc aggtggtgcc accaccggtg
     3481 aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac actgaaactg
     3541 gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg gagtgcagcc
     3601 acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat ttacgcttac
     3661 ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa cctgtcggca
     3721 acgcgtttct ccgactttgc tcaaaagtca tgtgataaca aaggggtgaa ctatggccag
     3781 tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat tgactgaaat
     3841 taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga cggatgatga
     3901 gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc aaagtgaaga
     3961 agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag atcctgaaat
     4021 caccatcacg acttgggagg aatggaatag caacagaaaa taaatggttt ttgggcaata
     4081 atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc taaccttatg
     4141 atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc ttttgagcga
     4201 gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag aagtccatgc
     4261 cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg aaagggaact
     4321 gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta gtaaaaccgt
     4381 caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc tggtgatgga
     4441 ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc agcaaattgc
     4501 gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata actcgctctc
     4561 cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg ctgaacaacg
     4621 gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca atatcgacgc
     4681 catcgtcgag catattcagc agcgtctggc ctcgcatcaa ctgcagcctt ctctgttgca
     4741 cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca ttttcgaccc
     4801 tgcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc atactgaaca
     4861 gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg atttccttga
     4921 acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat ttggcggtca
     4981 gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat atgggttgag
     5041 gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga aaagaacaca
     5101 taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg caggaaaata
     5161 gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc ctcttcccct
     5221 ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg cgcctggcga
     5281 ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat gaaaatcacc
     5341 cagaacataa cgttcacgcc accattaaaa ttaggggtcg gggagttata ccagaacaga
     5401 ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa gtcgagcatg
     5461 accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag caaagaaatc
     5521 aatgtggaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt caaaacagca
     5581 atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt cgtttcactt
     5641 agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat aggcgcttaa
     5701 tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg taatgtcaac
     5761 cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg actcagaacc
     5821 tttacgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata tctcccgtcg
     5881 taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc tttggtacgc
     5941 ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta ttgcccgtgc
     6001 catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag ctgttgcgtt
     6061 atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac atatgctgga
     6121 aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct cggctgaaaa
     6181 actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa aactgggcgt
     6241 tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg cttctaaagc
     6301 agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc cacgttttgt
     6361 attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc ttctcggtta
     6421 atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc attcaactga
     6481 cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt gcactgtata
     6541 aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg cggaaggtca
     6601 ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga cgcccgtgct
     6661 ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt tgttaccgga
     6721 catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa aaccgatcgc
     6781 ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg cgctgctggc
     6841 aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc tacaaggaat
     6901 ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg gtgttcccct
     6961 gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa agggcaacca
     7021 actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc cccattcatt
     7081 acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc agatccagtt
     7141 tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc tttctggaat
     7201 aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg cgccaacact
     7261 atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga actttccatt
     7321 aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga cccgccataa
     7381 acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg tttttggcct
     7441 tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg tacagtggtt
     7501 taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc cgttagtctt
     7561 cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag gcaaaatcag
     7621 ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg tcggtgtgct
     7681 ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg cagaaactgc
     7741 acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga gcgttccgca
     7801 gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag ccaatccgac
     7861 gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat tgaaactgct
     7921 gaaggatgat gcgccgaaag gtgaacgcgt cttagccgct atcgataccc tacaaagctg
     7981 ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc tggctctaat
     8041 gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa gtttcgttgt
     8101 cgcgtcctac ctcggtctgc tgattatgtt tgcagtgcat ggcattctgc tgggcattaa
     8161 tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt ttgcctttac
     8221 cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc gtcgtctggg
     8281 cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg gtcagaacgg
     8341 ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg ttggcattaa
     8401 cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta gttccgcagg
     8461 cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc ctgcgatggg
     8521 cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg acatgggccg
     8581 tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc agtggctgaa
     8641 gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac accgttaatt
     8701 cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt aaatgttcca
     8761 tcgcggcgat gcatactgac tcatatttaa acagatcgtc agcgaaaaaa atgtccaact
     8821 tatcaataca ttcctggctc aatgaatgtg cctgtagtga gaagcttaaa acctcatacc
     8881 cgagacgttt tctaagtgaa ctatctggaa tactcataat atcttcaagc acaccacttc
     8941 ccattcccgg aagatttagt ggattaaaaa gaagattgat attaagcatt gatattatcg
     9001 aaggaaactt taggagactc tcattttttt tgaatcgcag caaaaatgct cccggcagga
     9061 tgtatataat ttttttcata gtatatagtc cttaaataat gaataaccaa cctatttttt
     9121 atattgatca aattcgtcat tttttcgggt cggaattcac aggtaaaatc catcccaaca
     9181 aaattctcat taatatctct tatccgaaac tcactgccat aaagattatt aattatcact
     9241 ctatatttaa cattgtcctg cagatacgta tcaaaactaa gtgctggagc tcctggcttt
     9301 gaggcatcat agttaatgtt agacatatag gcaatcatac gattgaagtt cgactctgct
     9361 atttcatcgc agtcacattg acccgttgat ataaagaaag tgagtgccgc catggtagac
     9421 tcaagccttt ctttaaaaga gtcgaaagcc aggcttttaa tatttaaatc accataatta
     9481 ctctgtatta agtttgtaga aaacatctcc cgccccatat tgttaacaaa attattatct
     9541 catttaaatc taagtcactt acaatataag tttaagagcg acgccacagg atgaactatc
     9601 aaaaatagct catcatgatt agcaaaactt aaccatttta aaataaataa acaattaaag
     9661 aaaaaagatc acttatttat agcaatagct cgtcaaaggc agctttttgt tacaggtggt
     9721 ttgaatgaat gtagcaacga aatgcagaat ttcaggtcat gtaactcccg gcaaaaccgg
     9781 gaagtatgta atccttactc agtcacttcc ccttcctggc ggatctgatt tgcccaacgt
     9841 tgggcagatt caggcacagt aaacgccggt gagcgcagaa atgactctcc catcagtaca
     9901 aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat ctttatcgca
     9961 tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg gttttgctgg
    10021 cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaattag aaaaactgta
    10081 gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac accgtttcca
    10141 gaatagtctc cgaagcggga tctggctggt ggtctatagt tagagagttt tttgaccaaa
    10201 acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac ataacgaaaa
    10261 gaacctacat cagcaccagt caccactaca cgattccagc gaagcgaaac cggggatgga
    10321 ctcactggca cctgaggacg gatctcatcg tccagcggct gaaccaacac cgccaggtgc
    10381 acaacctacc gcccctggga gcctgaaagc ccctgataca cgtaacgaaa aacttaattc
    10441 tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc agggcgtgcg
    10501 catcgccgat gatcaaaact cactgcgtgc cggtaaccgt ggtccaacgc tgctggaaga
    10561 ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg aacgtattgt
    10621 tcatgcacgc ggatcagcag ctcacggtta tttccagcca tataaaagct taagcgatat
    10681 caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg ttaggttctc
    10741 taccgttcag ggtggcgctg gctctgcaga taccgtgcgt gatatccgtg gttttgccac
    10801 caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc caatcttctt
    10861 tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag aaccgcactg
    10921 ggcaattcca caagggcaaa gtgcccacga tactttctgg gattatgttt ctctgcaacc
    10981 tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg
    11041 caccatggaa ggcttcggta ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac
    11101 gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt gggatgaagc
    11161 acaaaaactc accggacgtg acccggactt ccaccgccgc gagttgtggg aagccattga
    11221 agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag aagatgaatt
    11281 caagttcgac ttcgatcttc tcgatccaac caaactgatc ccggaagaac tggtgcccgt
    11341 tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg ccgaaaacga
    11401 acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca acgatccgct
    11461 gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg gtgggccgaa
    11521 tttccatgag attccgatta accgtccgac ctgcccttac cacaacttcc agcgtgatgg
    11581 catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact cgatcaacga
    11641 taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat catatcagga
    11701 acgcgtggaa ggaaataaag ttcgtgagcg cagtccatcg tttggcgaat attattccca
    11761 tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg tcgatggttt
    11821 cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct
    11881 ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta tcgaactgac
    11941 tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc
    12001 cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag cgattttact
    12061 taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggccctga aggccaaagg
    12121 cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg acggtacggt
    12181 gttgcctata gccgctacct ttgccggtgc accttcgctg acggtcgatg cggtcattgt
    12241 cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact acctgatgga
    12301 agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac
    12361 aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg ctgacggtag
    12421 ttttatggat gaactgctaa cgctgatggc agcacaccgc gtgtggtcac gcattcctaa
    12481 gattgacaaa attcctgcct gatgggaggc ggcgcaattg cgccgcctca aatgatttac
    12541 atagtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaactgt
    12601 gcaaattcaa tatattgcag gaaacacgta ggcctgataa gcgaagcgca tcaggcagtt
    12661 ttgcgtttgt cagcagtctc aagcggcgca gttacgccgc ctttgtagga attaatcgcc
    12721 ggatgcaagg ttcacgccgc atctggcaaa catcctcact tacacatccc gataactccc
    12781 caaccgataa ccacgctgag cgatagcacc tttcaacgac gctgatgtca acacatccag
    12841 ctccgttaag cgtgggaaac agtaagcact ctgacggata gtattatcga taaacgccgg
    12901 atgacacatc acctccagcg aacgatcacc ccgatggctt gcgtcatcca gcacttgcag
    12961 gaacagtgcc tcgctaatct cttcaccata aaacgcgcta ctgaatccct gagaactgcg
    13021 cagattagcc ggtaaatcac cagagttaga tagcggctga cggtcgatgc gcaacgcaat
    13081 cccctcttca gccgcaaacc tggcaacaat cgggaaaatc tgcgggaaca tatgcacatg
    13141 atgatggcta tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg
    13201 actggcaagc tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca
    13261 gatccatttt cccagcacac catcgcgggt taaccccggc atagctgtca gtggcttgcc
    13321 catagtaagg acaaagtaca tccctatggc cagactcggt tcatcacgac tcaactgcac
    13381 cgcatggtca atagcctgcc cattcaccag cgccgtcgtc gatgtgacaa tcccattgcg
    13441 acaggcctcg ataatgccgt agttctgacc tttgcttaag ccaaaatcat cggcattaac
    13501 aatcagtaag cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca
    13561 gtcggcaaaa tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc
    13621 agcatcgcga tcggaatgca ccaacggact aaggtttagc gccagtaaca catcgttaaa
    13681 ttctccgcta agtgcggcgt tgctggcagc aatctcgaag cctttaatgg tgtgaatcag
    13741 ccccatcact ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc
    13801 cagcttacag gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg
    13861 cgggatatta acgtaatgtt cagcttgctt gtcgttgtag atagcgttga tcacttcgca
    13921 cgctgcatca gagtaataag ccccaccgcg ctgttccagt tctttcggct taactttcaa
    13981 ctccgggttt ttatacagct caaaaagttg tttctcgact ttctgtacta cctgcgctcg
    14041 tgcgccgcct ttgtagtatt cgcccatttc aatagccagc atctcttttt gcttgaagta
    14101 ataaagcaaa taagaacacg gcaacagatt cagagaacga attaagcctt cgctaaatgg
    14161 cagatcgaaa atatttttaa cgccagatgc ttttaactgc ccggatgcca caccatcaag
    14221 caattcggca aagcgcgact tgccatttac cagcacatcc ttaatgaaca ccatatggtt
    14281 gaggccgaac aaatcgatag ataaatcatc gctgtctttc agcatcagaa catcgtgaat
    14341 aaacatcttc atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg
    14401 acgataaacg gcttcagtaa ccattcccgc cgggttagtg aagttaatca cccatgcatt
    14461 cggacaaagt tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc
    14521 tttaaacaga ccgcccgcgc cgttggtttc ctggccaaga taaccatgac ttaatggaat
    14581 acgttcatcc agttcgcgcg ccggtaattg gcctacgcgc agttgggtag taacgaaatc
    14641 agcatctttc aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc
    14701 gttatcaatc atccgttggc agagttcaaa aatgatatcc agtttctctt taccatcttc
    14761 gacatccacc agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc
    14821 cagtaactcc ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct
    14881 cataatttct cccttcagta cagaatactg atatctggca tatctgcccc cccggacata
    14941 aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat
    15001 tcagtcaatt tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta
    15061 ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg
    15121 gtcatttcca gttgtttttt ggcaaaatta atacggatct cattaataat ctgcattggt
    15181 gttttgccat aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac
    15241 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
    15301 gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa
    15361 cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa
    15421 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
    15481 tgcgaagcga caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta
    15541 ctgatcccaa cgttcaatat acgcgtggca ccaaactcat aaaaactttg atggtgcgaa
    15601 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
    15661 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagcccg
    15721 ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc
    15781 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
    15841 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
    15901 cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc
    15961 cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
    16021 ctgatccatc atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt tcagtgccgc
    16081 atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
    16141 ctcttcagct tccgtttgcg tatcgggaat gttatcgaga tccatcatac atcgttcctc
    16201 tctattctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg
    16261 atatcttctt cgcgctcttc tttatcaatc gcgttttgtg ctttgttagc caccacaaca
    16321 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcaaccag caatgcggcg
    16381 acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata
    16441 ttggtcaccg gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc
    16501 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
    16561 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
    16621 taatcagcac ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg
    16681 cccccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
    16741 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
    16801 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
    16861 aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc
    16921 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
    16981 gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag
    17041 gaacgcgata ctgaagcagg tacgctatcg ggcaatttaa tgacccaatt gcggcggaca
    17101 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgct
    17161 ccacctaacc agtttgcgcc aaccacatag gcctcaccga cactatatgg ggtgacggtc
    17221 ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc
    17281 agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat
    17341 acgttgccgc caataccttt caggccatta agtgtttcaa tggttgaggc gtcgaggcga
    17401 atacctaagg aataaaaaaa cgccccctcc ccaaagctta gaaaaacgtt gttaattaat
    17461 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
    17521 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
    17581 gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata ttgcggtatt
    17641 gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc tgcggctttt
    17701 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
    17761 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
    17821 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
    17881 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
    17941 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
    18001 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
    18061 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
    18121 acggtataga cctgagcaat agtatttctt tgaccttata aattgtttct ccttgacctg
    18181 tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg
    18241 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaattga
    18301 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
    18361 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
    18421 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
    18481 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
    18541 ctgtacaaac tggtctttag tacgatcata agccgtacaa cccgccagca tggttaatac
    18601 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
    18661 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgcgaa cgaaacggct
    18721 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
    18781 gtttcagtgg caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
    18841 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
    18901 gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta tccgttcatt
    18961 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg aaagctgtgc
    19021 cagatggcga ttaatgagct gcgcctggaa accggcaacg aatcactgca atttattgcc
    19081 gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
    19141 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc cagcgaacag
    19201 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
    19261 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
    19321 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacagac
    19381 attaatccgc tgtatcgtct caacaaacgt cagggtaaac agttactggc ggcattaggt
    19441 tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga tcgcccttct
    19501 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
    19561 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
    19621 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacat
    19681 aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
    19741 gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct gacaggcgta
    19801 gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg ctccccgcct
    19861 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
    19921 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
    19981 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
    20041 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
    20101 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgta ccgcaggcga
    20161 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
    20221 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
    20281 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
    20341 taggcgcgca gctttgcaag cattgcagac catcgctgat gaacaaaaac tttgttatgg
    20401 cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg
    20461 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
    20521 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga
    20581 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
    20641 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
    20701 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
    20761 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
    20821 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcaattta
    20881 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
    20941 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg tgtcatgatg
    21001 ttgaaatcca tcgacatctg cccttccgtc agtttcgctt ttaccacctg gtccgctgca
    21061 aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc
    21121 atctcgccgc cttccagcaa cgtcactatc ctttccatgc cgggaaacaa aga
//
LOCUS       DAJTVE010000069        21172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000069 DAJTVE010000000
VERSION     DAJTVE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO439_002420"
     CDS             complement(<1..200)
                     /locus_tag="OO439_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6316770.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALA"
     gene            complement(197..1758)
                     /gene="casA"
                     /locus_tag="OO439_002421"
                     /pseudo
     CDS             complement(197..1758)
                     /gene="casA"
                     /locus_tag="OO439_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1856..4555)
                     /locus_tag="OO439_002422"
     CDS             complement(1856..4555)
                     /locus_tag="OO439_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6316771.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4750..4902)
                     /locus_tag="OO439_002423"
     CDS             complement(4750..4902)
                     /locus_tag="OO439_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6316772.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5167..5901)
                     /gene="cysH"
                     /locus_tag="OO439_002424"
     CDS             complement(5167..5901)
                     /gene="cysH"
                     /locus_tag="OO439_002424"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6316773.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5976..7688)
                     /gene="cysI"
                     /locus_tag="OO439_002425"
     CDS             complement(5976..7688)
                     /gene="cysI"
                     /locus_tag="OO439_002425"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6316774.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7688..9487)
                     /gene="cysJ"
                     /locus_tag="OO439_002426"
     CDS             complement(7688..9487)
                     /gene="cysJ"
                     /locus_tag="OO439_002426"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6316775.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9806..10168
                     /gene="queD"
                     /locus_tag="OO439_002427"
     CDS             9806..10168
                     /gene="queD"
                     /locus_tag="OO439_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6316776.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10246..11517
                     /locus_tag="OO439_002428"
     CDS             10246..11517
                     /locus_tag="OO439_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6316777.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11508..11768
                     /locus_tag="OO439_002429"
     CDS             11508..11768
                     /locus_tag="OO439_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6316778.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11785..12360
                     /locus_tag="OO439_002430"
     CDS             11785..12360
                     /locus_tag="OO439_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6316779.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12508..13368)
                     /locus_tag="OO439_002431"
     CDS             complement(12508..13368)
                     /locus_tag="OO439_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6316780.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13365..14144)
                     /locus_tag="OO439_002432"
     CDS             complement(13365..14144)
                     /locus_tag="OO439_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6316781.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14122..15531)
                     /locus_tag="OO439_002433"
     CDS             complement(14122..15531)
                     /locus_tag="OO439_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6316782.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15553..17007)
                     /locus_tag="OO439_002434"
     CDS             complement(15553..17007)
                     /locus_tag="OO439_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6316783.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17077..17893)
                     /locus_tag="OO439_002435"
                     /pseudo
     CDS             complement(17077..17893)
                     /locus_tag="OO439_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            18211..19488
                     /locus_tag="OO439_002436"
     CDS             18211..19488
                     /locus_tag="OO439_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6316784.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19515..21000
                     /locus_tag="OO439_002437"
                     /pseudo
     CDS             19515..21000
                     /locus_tag="OO439_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5092 a         5609 c         5214 g         5257 t
ORIGIN      
        1 gccagtgcgg tgatacgcca ctcaggttca tcctgccact ttatttgtct gttcttcaga
       61 aacaggcgaa atccatccgt cagacaaaca tcattaaccg tcgtactacg ccgcagactg
      121 gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt ggctttgtgc
      181 tcctctttta caatactcat tcagcagcct cctgcttttt ctccctggcg gctttagcac
      241 tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg cgttccaaat
      301 caacgggctc ataaggattg gtgaatacac gctcgtcaaa atcctgacgc gcgaataacc
      361 aaatttcctt attccatttg ctgagtaatt catccgcatc ctgaccttct tcaatttggc
      421 gcactaacct caggaagcga tgctgagttt tgttccagaa gtcgatatcc acaaaactaa
      481 aatcacccct tgcacctttt ggatcggaga accatgcttc tttcaatgca ctccgtaaca
      541 gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag tttcggtatc tggccttctt
      601 tttattgagc agcagcggga aatgatgttc gtaccaacag cgcgctttca tgttgtcgaa
      661 atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg cattaaagag
      721 tttcaccacc agcgcgggaa gttccgtatt gttttctgac ttacccgttt cgataaggcc
      781 taaccagtcg cgccagatta aaccgcccgg ttgtggtttg accgagtaaa actcgccgcc
      841 ctctttaagt agtacacggt aaggcgttaa gggatgctgc cacatggcat agttagcgcc
      901 gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc tgctcaccgc aaatatcgca
      961 gttgccgact gtggtggtat taaaatcaat acgaatacgg cgaggcattc cccagtacgc
     1021 ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc tgatgcgcgt
     1081 cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta aatcggcttc
     1141 atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt gattaccctg
     1201 atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac cggtgcggta
     1261 gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg cagagcaatg
     1321 tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa acttcgttgt
     1381 ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc cggtgagcgc
     1441 ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac tggaaagcat gttctaatga
     1501 cagcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat catcccaacg
     1561 acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact gccataccgc
     1621 accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg ccagatcgac
     1681 tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg gggttgtcag
     1741 aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg tcggttgtcc
     1801 atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag acatctcaac
     1861 caaccttgcc aatcaatcct tccgtagccg aatacccaca actttcgtca tcgttgacta
     1921 aaataacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc ctgaacgcct
     1981 ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg cgcactctga
     2041 caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca ccgtcaatac
     2101 aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt ttttctggga
     2161 ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac tttttagcca
     2221 ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca aatccttccg
     2281 gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg tgtggcatct
     2341 gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca tgatcgggat
     2401 agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga gagtcgtccc
     2461 agacgggagc cagaatcagc aattcagggg gagaacgctc gtcctttccg tcacgcttta
     2521 actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga atcagcaaat
     2581 caacaggggc caggtcggag atcatttcgt ccaggtcaca atcgacactc tgctctaata
     2641 cctgagtaca aataaggact tttccggcac gctgtgagct atcttcttta ccaaagcgtg
     2701 ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta tgaaaaagtg
     2761 aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc ttaatagcgt
     2821 catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc gattcgatac
     2881 gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc tcaacgtctt
     2941 tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc agccaggggt
     3001 aatcatcctt ttccaggaac ggagcttctt gcaggccctc tgtaccccgc gcaaaggcag
     3061 cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata acgctgtttc
     3121 cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa gcgtcacagg
     3181 cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc agattattat
     3241 gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca atttcagcca
     3301 gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg aacgtttgtt
     3361 catccggttc ttctgacccg actaaatcac cagaccagag tgattcatta aagcggtcca
     3421 ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc gaatagaaag
     3481 caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt gttggcagac
     3541 caaaaaacag cccctgagcc ttccccgcag ctatcaacct gtgcgccagg ataagcgccg
     3601 cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt ccttgctggc
     3661 tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata aaaggaaaca
     3721 gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg gttaaggcgt
     3781 tttgagcctg aactaaagtt ttctgccagt aacctttaat atccattggg tgtgcgacgc
     3841 gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt gcagagatat
     3901 accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat gcggggatct
     3961 ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca gctttgtctt
     4021 caggcagaaa attatccaat tcatccatac gatcaggggg ccaaccatga tgccctgtcg
     4081 tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc gaagatgaag
     4141 gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc agcgttgaat
     4201 gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca gggggcaggt
     4261 aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc aaccaggcga
     4321 aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa tcagccagcc
     4381 cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc gaatgccagc
     4441 aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt ccccagaaat
     4501 caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta cgcatatatt
     4561 aaaaattatc tgtgatatat atcattcaat aaaatattta cattatgaat aatcaaataa
     4621 ttttcatccg taatatatat gaataataaa cataggcata atattcacat aaataatata
     4681 aattaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg ttttaccccc
     4741 gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca taccacgttc
     4801 tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca gtactgtaaa
     4861 acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc tccctgcaaa
     4921 gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg atagaaatat
     4981 cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac agcctcaagc
     5041 acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta aaatcaggaa
     5101 tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta atttatccgg
     5161 caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac gtgtttcttc
     5221 ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg ataaatatcc
     5281 ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat aaatagttcg
     5341 gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg ccagcaccgg
     5401 taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg tttgcgcgtt
     5461 aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt acttttcaat
     5521 gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg ctgcgctttc
     5581 ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt caataaagcg
     5641 gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat ccgggcgaat
     5701 ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag aaagtacata
     5761 ttcaccgggc agattatcca gcgcccaggc tacgcggcct tcagcgtcca gtttttccag
     5821 ttcggcgtta gtttccgcca gcgccagaat acgatctacc ttcggcagtt cgttcagggc
     5881 gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg cgacgctcac
     5941 gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
     6001 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
     6061 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
     6121 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
     6181 aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat cgcgcgacca
     6241 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
     6301 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctctgc ttccgccatc
     6361 gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg cggcgtgacg
     6421 gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc gctttccggt
     6481 acaccggtga tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc
     6541 gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat gcgaccattt
     6601 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatcg
     6661 cctcgtccgg tgaactcata tggacggatc ggttcaaatt tgatccccgc gcgacgctcc
     6721 acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt tttggcattt
     6781 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc
     6841 agcgtatgct ccagcggcag atagccaaac tcgctcgccg tgcgggcgta ggttttcttg
     6901 ttgccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcctac cagcttgccg
     6961 ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc gttctgcggc
     7021 gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag gatcggttct
     7081 tcatcagtag tggcgacttt ttcctggtcg agccagatct ccgcatacgc gcgggtacga
     7141 ggcaacagat gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac
     7201 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagctgt cgccagcgca
     7261 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
     7321 tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
     7381 gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg cagacgacag
     7441 cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc acggatgtcg
     7501 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttgtc gcccttaaag
     7561 ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
     7621 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
     7681 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
     7741 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
     7801 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
     7861 tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacataa actttttctt
     7921 tttggtcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg acgtagcgct
     7981 gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag aacagccagt
     8041 ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa ggcacggaac ggcgcgatgc
     8101 cggtaccggg gccaatcata atcactggcg tttgcgggtt agcgggcagg cggaaattgt
     8161 cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcgatcc gcgaggaagc
     8221 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga
     8281 cgtgtacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg cgcggggtca
     8341 gcgggcgcag caggttaatc agcgcttcgg catccagttg cgccggagag aaacgcacca
     8401 tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
     8461 gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg gcggtgttga
     8521 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
     8581 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccgggt
     8641 cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggcct gaatcgccta
     8701 agtcaatttc gatatgacga acgtcttttt cagagttacg cccggtaatt ttctggttaa
     8761 ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta
     8821 ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
     8881 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatcg gcatcgacac
     8941 ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcgaaatct ttcccggact
     9001 ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca
     9061 gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
     9121 ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg atttgtttga
     9181 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat aaatcatcgc
     9241 gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata atagttatac
     9301 ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc tgattgagta
     9361 cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg gtcgtggccg
     9421 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
     9481 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
     9541 aaaacagaag agaagataag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
     9601 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
     9661 taaccaaccc aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt
     9721 gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt
     9781 tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
     9841 agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca
     9901 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
     9961 cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta
    10021 tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa aatggatttg
    10081 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
    10141 cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag
    10201 taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga agacgactgc
    10261 gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga
    10321 gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc
    10381 ggcgggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca tctgaccgcg
    10441 cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgcccga tggcgtaacg
    10501 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat
    10561 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg agcgacggtg
    10621 gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga cgatattctc
    10681 cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg
    10741 ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg
    10801 gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg
    10861 ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa
    10921 acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg tgttccggca
    10981 agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat caaaaacacg
    11041 gaatcactgg agtatggtgc gcatctggag ccagaaggtg gcttgcacag tatgccggtg
    11101 caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc
    11161 ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc ggcacaaacg
    11221 ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac
    11281 gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc ggcgcttttg
    11341 caacgccctg gctggtatcg ggcgtggcct gcgttaatgc aggatatttc ccgcgattta
    11401 tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg tcatttacgt
    11461 cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg tctgtagccc
    11521 ataatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc gttgagcgtc
    11581 agatggtaca acggttgatt aacgcctgtc cggcaggtct tttttcgctc acaccggaag
    11641 gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg
    11701 acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgct
    11761 ttggataaat aataaggata atttatgccc ctcttacacc tactccgcca gaatccggtg
    11821 attgctgccg ttaaagataa tgccagcctg caactggcta tcgattctga atgccagttt
    11881 atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa gattaagaac
    11941 gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtc
    12001 gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac caaagcctca
    12061 atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt tattgttgat
    12121 tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag
    12181 atcctgccag gcggtatgcc caaagtgctg ggctgggtga cagagaaaat ccgccaaccg
    12241 ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt
    12301 gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa attactttga
    12361 cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc acatccgaca
    12421 ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc caggccagaa
    12481 ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt ttgtcaccag
    12541 tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg aaaataccgc
    12601 agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa
    12661 cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga
    12721 aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc gactgtagcc
    12781 cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg cctctccgcc
    12841 ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa catttttcag
    12901 atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca gttgttgcat
    12961 tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg aaaggcatag
    13021 cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac
    13081 gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca
    13141 ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa caacgtccgc
    13201 aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca ctacaggctg
    13261 aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg ccagccagct
    13321 tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg ctgcatcctt
    13381 tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg cccatcaatc
    13441 agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcatt
    13501 tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg ctggcgcatt
    13561 cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga
    13621 acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa cagcgcgtcg
    13681 agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc cagcaaaaat
    13741 ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga
    13801 ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct
    13861 gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag
    13921 tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt
    13981 ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa acttcgcagt
    14041 agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt ttccgccagc
    14101 attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt
    14161 tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg tcacaacaaa
    14221 atcaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc actgcgccag
    14281 cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg cggtggcaaa
    14341 accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag
    14401 attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact
    14461 gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa aacttcccag
    14521 caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg cgcccacaat
    14581 taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa tagtctgggc
    14641 gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga caaagaagac
    14701 gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga tgtgtttatg
    14761 ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc gatgcacgat
    14821 agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt ctggcgttcc
    14881 ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta gccagcgcca
    14941 ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca catagcctac
    15001 ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg gggaaaattc
    15061 agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa tcaaaatgcg
    15121 cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag caagcgtaat
    15181 cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga tccacccaag
    15241 aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc ccgccataaa
    15301 cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga cgccgagaac
    15361 ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt gaaaacggtt
    15421 aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt cattgtccgg
    15481 taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca tcgcggaacg
    15541 ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa tgccattagg
    15601 atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct
    15661 ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc cgaggcgaat
    15721 ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt cctcttccgg
    15781 cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt tctgatagct
    15841 atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact cagtacgaat
    15901 acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg acacttcagt
    15961 ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca ctttatccgg
    16021 tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc gctggcattg
    16081 cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa tgcgagggtt
    16141 accttcagcc ataaagatca gcacgcattt tccgtctgca aaatgggtga agtgttgggt
    16201 gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca ccatgacttc
    16261 acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata tagccgtaga agaggttgtt
    16321 ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa gtgcaccttc
    16381 gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca cgtttttaat
    16441 gcgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat cttcgattgc
    16501 gccgtagagt gtggagaact gcccgatact gcgggttgct accaagccgc ccatctgcgc
    16561 cagcggcctt gactgcggag aatgccccgt ggtgtaacct ttttcacgca acgcattttc
    16621 cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc atattctcaa tatcaatatt
    16681 aatgatttga ttcatggcgg agccgtcaag caccaccgag ttttccacaa cggtttccag
    16741 cccaccttca gtggcggaag cgccggtacg cggcacaccg ttaattttgt gcgcattcat
    16801 aaaattcagc acacgggata cttgctctgt ggaaccgagt tttacgaccg ctgccggaat
    16861 cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa tactgttctt
    16921 ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct ttagttggtc
    16981 gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa aatggaataa
    17041 tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat aaccaccatt
    17101 gaccactaat aaatgcccat tgacataatt tgaagccgga cttgcgagga atacagctgc
    17161 gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa gaacgcgctg
    17221 attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggagc
    17281 aataccattt acctgaatat tatattgacc tagttcatca caataagctt tggtgaatcc
    17341 ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa
    17401 caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt ttgcagcttc
    17461 atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc agtcggcacg
    17521 accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca gaatatcaac
    17581 tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
    17641 atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct ttgtttcgcc
    17701 gttatctttc aatcatttcc tttgtttcgc cgttatcttt gacgaaacta ggaataaaga
    17761 tatttgcgcc agctttggcc aacgccatgg caaatgcctg gcctaaaccg ctattcccac
    17821 cggtaacaat tgcggtttta cctttcagtg agaaaaaatc cattgagaac gcattgagag
    17881 attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaagaaag gtaacttccc
    17941 ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat ttttagcata
    18001 acggcataac tgctaatgtg atcgtaatca cagtgtgata ttcgtattgt caaatagttt
    18061 taaaaagcag aagcgtgatt actctcacac aacacctctg ctttccatgt tatttcttat
    18121 tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta cttttctctt
    18181 ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg ccgttggata
    18241 accctcgcga taattagttt tagcggcggc gttagttttg acctggctta tttacgttat
    18301 atttttcaaa ttcccatggc gaaatttatg ggattcagca ataccgagat aggtttaata
    18361 atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt tattgccgat
    18421 aaattttcac accgcaaaat gattacttcc gcgatgatca ttaccggatt actgggtctg
    18481 ttaatggcaa cgtatccacc gctgtgggta atgctctgta ttcaggtcgc ctttgcgata
    18541 acgacgattt taatgctgtg gtcggtgtcg attaaagccg cctcgttgct gggcgatcat
    18601 agtgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg
    18661 ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag cgccagcctg
    18721 aaaacagtca ttatcatcta cagtgtggtt tacatcttgt tggggattct gtgctggttt
    18781 tttgttagcg ataacaacaa cctgcgcagt gccaataacg aagaaaaaca gtcattccag
    18841 cttagcgaca tccttgccgt tttgcgtatc agcaccacct ggtattgcag catggtgatt
    18901 tttggtgtct tcacaatcta cgccattctg agttactcca ccaactatct gaccgaaatg
    18961 tacggcatgt cgctggtggc agcgagctac atggggattg tgatcaacaa aatcttccgc
    19021 gcgctgtgcg gcccacttgg aggcattatc accacctaca gtaaagtgaa atcccctacc
    19081 cgcgtgattc aaatcctttc catcatcggt ttgctggcgt tgacagccct gctcgtcacg
    19141 aactctaacc cccaatcggt cgcgatgggg attggcctga ttttactgct gggattcacc
    19201 tgttacgcct cacgcgggct gtactgggcc tgccccggcg aagcgagaac accgtcttac
    19261 attatgggca ccacggtagg tatttgttcg gtgattggat tcctgccgga tgtcttcgtt
    19321 tacccgatta tcggccactg gcaagacacc ctgcccgctg cagaagccta ccgcaatatg
    19381 tggctgatgg gcatggcggc gctcggcatg gtgattgtct ttaccttttt gctgttccaa
    19441 aaaattcgta ctgctgatag cgcccccgca atagctagca gcaagtaagc caggaaaatt
    19501 acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga agtcagagca
    19561 caaaagtggt gatgtacgat ctggaaggta atgtggtttg cgaaggtaaa ggcttattac
    19621 agccgatgca cacgcctgat gccgatactg cagaacatcc tgacgacgat ttatgggcat
    19681 cattatgttt tgcaggtcac gatttgatga gccagtttgc cgggaataaa gaagatattg
    19741 ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt attgaaagcc gacggcacgc
    19801 cagctgcgcc gttgattagc tggcaggatg cacgcgttac acgcccttac gaacatacga
    19861 atcctgacgt ggcgtatgta acctcttttt cgggttatct gacgcatcgc ttaaccggcg
    19921 aatttaaaga taatatcgcc aactattttg gtcagtggcc ggtggattat aagacctggg
    19981 catggagcga agatgccgca gtcatggaga agtttaatat cccccgtcat atgctgtttg
    20041 atgtgcaaat gccaggcact gttcttgggc atatcacacc tcaagccgcg ctggcgacgc
    20101 atttcccggc aggactgccg gttgtttgta ccaccagtga taaaccggta gaagctctgg
    20161 gagccggatt actggatgat gaaactgcgg taatttctct tggcacttac atcgcattga
    20221 tgatgaacgg caaagccttg ccgaaagatc cggtggcgta ctggccgatt atgtcttcta
    20281 ttccgcaaac attgctgtat gaaggttacg gtattcgcaa aggcatgtgg acggtgagct
    20341 ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc cagggcgcag gatctttcac
    20401 cggaagattt actcaacaag aaagcctcta gtgtgccacc tggctgtaat gggctgatga
    20461 cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcgggatt atgatcggct
    20521 ttgattccag catggattac gcatggatat atcgttcgat attggaaagc gtggcgctga
    20581 cgctgaagaa caattacgac aatatgtgta atgaaatgaa tcactttgcg aaacatgtaa
    20641 tcattactgg cggcggttcg aacaggatct gtttatgcag atttttgccg acgtgttcaa
    20701 ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc ctgggggcag cgattaatac
    20761 aggggtaggt ctggggctat acccggatta cgcaacggct gtcgataaaa tggttcgcgt
    20821 gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg
    20881 cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt atgaagtgat
    20941 gcatggggaa ttggggaatt ggggaatgcg gattcgatcc agagctggtc gaatgcgtga
    21001 ataaggtagg ccgggcggaa aactgcccgg cctgaacata cctgaatggt tatccccgct
    21061 ggcgcgggga acataagttc gacaacttaa aaaatgataa caagtccggt ttatccccgc
    21121 tgatgcaggg aacataataa aaactttaac tagttcggtt tatccctgtt tt
//
LOCUS       DAJTVE010000070        21078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000070 DAJTVE010000000
VERSION     DAJTVE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(17..562)
                     /gene="hemG"
                     /locus_tag="OO439_002438"
     CDS             complement(17..562)
                     /gene="hemG"
                     /locus_tag="OO439_002438"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6316785.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(574..2025)
                     /gene="trkH"
                     /locus_tag="OO439_002439"
     CDS             complement(574..2025)
                     /gene="trkH"
                     /locus_tag="OO439_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6316786.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2064..2678)
                     /locus_tag="OO439_002440"
     CDS             complement(2064..2678)
                     /locus_tag="OO439_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6316787.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2678..4009)
                     /gene="pepQ"
                     /locus_tag="OO439_002441"
     CDS             complement(2678..4009)
                     /gene="pepQ"
                     /locus_tag="OO439_002441"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6316788.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4199..6388
                     /gene="fadB"
                     /locus_tag="OO439_002442"
     CDS             4199..6388
                     /gene="fadB"
                     /locus_tag="OO439_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6316789.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6398..7561
                     /gene="fadA"
                     /locus_tag="OO439_002443"
     CDS             6398..7561
                     /gene="fadA"
                     /locus_tag="OO439_002443"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6316790.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7751..8452)
                     /gene="fre"
                     /locus_tag="OO439_002444"
     CDS             complement(7751..8452)
                     /gene="fre"
                     /locus_tag="OO439_002444"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6316791.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8499..9992)
                     /gene="ubiD"
                     /locus_tag="OO439_002445"
     CDS             complement(8499..9992)
                     /gene="ubiD"
                     /locus_tag="OO439_002445"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6316792.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10159..10647
                     /gene="rfaH"
                     /locus_tag="OO439_002446"
     CDS             10159..10647
                     /gene="rfaH"
                     /locus_tag="OO439_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6316793.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10644..11426)
                     /gene="tatD"
                     /locus_tag="OO439_002447"
     CDS             complement(10644..11426)
                     /gene="tatD"
                     /locus_tag="OO439_002447"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6316794.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11468..12244)
                     /gene="tatC"
                     /locus_tag="OO439_002448"
     CDS             complement(11468..12244)
                     /gene="tatC"
                     /locus_tag="OO439_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6316795.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12247..12762)
                     /gene="tatB"
                     /locus_tag="OO439_002449"
     CDS             complement(12247..12762)
                     /gene="tatB"
                     /locus_tag="OO439_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6316796.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12766..13035)
                     /gene="tatA"
                     /locus_tag="OO439_002450"
     CDS             complement(12766..13035)
                     /gene="tatA"
                     /locus_tag="OO439_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6316797.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13114..14754)
                     /gene="ubiB"
                     /locus_tag="OO439_002451"
     CDS             complement(13114..14754)
                     /gene="ubiB"
                     /locus_tag="OO439_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6316798.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14751..15356)
                     /gene="ubiJ"
                     /locus_tag="OO439_002452"
     CDS             complement(14751..15356)
                     /gene="ubiJ"
                     /locus_tag="OO439_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6316799.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15370..16125)
                     /gene="ubiE"
                     /locus_tag="OO439_002453"
     CDS             complement(15370..16125)
                     /gene="ubiE"
                     /locus_tag="OO439_002453"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6316800.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16220..17647)
                     /gene="rmuC"
                     /locus_tag="OO439_002454"
     CDS             complement(16220..17647)
                     /gene="rmuC"
                     /locus_tag="OO439_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6316801.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17788..18549)
                     /gene="udp"
                     /locus_tag="OO439_002455"
     CDS             complement(17788..18549)
                     /gene="udp"
                     /locus_tag="OO439_002455"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6316802.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18811..19623
                     /locus_tag="OO439_002456"
     CDS             18811..19623
                     /locus_tag="OO439_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6316803.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20017..21078
                     /gene="hcp"
                     /locus_tag="OO439_002457"
                     /pseudo
     CDS             20017..21078
                     /gene="hcp"
                     /locus_tag="OO439_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5184 a         5646 c         5292 g         4956 t
ORIGIN      
        1 tttttattct ttatgcttat ttcagcgtcg gtttgttggt taactgggcg atttctcggg
       61 cgaaattcgc cacctgctcc caatcggtat agacaacttc tttgcgcgta tccgtttcac
      121 cgcctgacat cttcataatt agcttgatca taaaacgatc gtaccagcga taacgtgggt
      181 aacgcagcgc cccggcaatg accgcgcagc gatcgggacg ccattgcgag ttcatcagaa
      241 acttccgcgc gtagctgttg gtctgtggag tacgcttctc cggtttgcgc gccaccaggt
      301 ttacggaata aaaggcgctc ggcatcgaat tcagtcgcgt cgcatgtttt ttgacaaatt
      361 cctggaacgc agagtggtag tgaccatagc gaatagaagc accaatgacc acacggtcat
      421 aattttccca ttgtggttct tcaatgcggt gcacattggc gacatccgtc tggatcccca
      481 gttctttcag ttccgaagcc aggtaggagg caatctcgcg cgtttgtccg tcccttgttg
      541 agaaaagaat taatgttttc acgtattact ccatcattca cgccagaaag tcggggtaaa
      601 gagcaccagc aatgtaaaga cctcgagacg accaaacagc atgttggcaa tcaggatcca
      661 tttagccacc gggttcatac tggtaaagtt atcagcaacc acgccaagcc ctggccccag
      721 gttattcaat gtcgcaacaa ctgaagcaaa ggcagaaaag tcatccacgc ccgtggcgat
      781 aatcgccagc atactgacaa taaacaccaa tgcataggcg gagaaaaatc cccaaacggc
      841 ttcgaggata cgttccggca gtgcgcgatt ccccagctta atgctataga cggcgttcgg
      901 atgcaccagt cgtttcagct cacggttccc ctgcttaaac agcagcagga tgcggatcac
      961 tttcaggcca ccgcccgttg acccggcgca accgccgata aaagctgaac ataaaagcag
     1021 taccggcaaa aagagcggcc agcgggcaat gctgtcagtc gtaaacccgg cggttgtcgc
     1081 catcgatacc acctggaaaa acgcctggtt aattgtcatc agcgccgaac tgtagacatt
     1141 atgaaaccac agtacgagtg tacaaataac caccagcgta aactgtacgc cgataaacat
     1201 gcgaaattcc ggatcgcgcc aataaacctt cagactacgc ccacttaaca gtgaaaagtg
     1261 cagaccgtag ttacagccgg agatcagcag gaagatagca atgatagtgt taatagtcgg
     1321 gctgtcgaaa taaccgatac tggcatcatg tgtcgagaag ccgccaatag cgatagtcgc
     1381 aaagctatgg ccgatggcat caaaggcatc cattccggca aaccacaacg ccagcgcaca
     1441 ggcgacggtc agcaagacat aaatcaacca cagggttttc gccgtttccg caattcgcgg
     1501 gcgcattttg ttatctttca gcgggccggg catttctgcg cgatagagct gcatcccacc
     1561 cacgccgagg ataggcagta tcgcaaccgc taacacgatg atccccatcc cgccaaacca
     1621 ttgcagcatc tggcgataaa agaggatggc atgagggagc gaatccagcc ccaccagcgt
     1681 agtggccccg gtggtggtca ggccagagaa agattcaaaa aacgcatcgg taatcgtgag
     1741 gttcgggctt tccgagaaga taaaagggag cgcaccgaca ctacccagca cggtccagaa
     1801 cagcaccact atcagaaacc cctcacggga tttcagttcg cccttctctt tacggttcgg
     1861 ccaccacagc atagaaccaa tggcgagggc gacaaaaaag gtctgggtaa aagcgcggcc
     1921 cgctccatcc cggtagatga gtgctaccag cccagggata atcatggtcc ctgaaaataa
     1981 gatgaccagt agtccaacga ttcgggtaat ggcgcgaaaa tgcatctctg ccgcttcctt
     2041 agttctgcaa aacgaagtgg ggattattct tcaatcgcta acaattgcaa tgaaccacgg
     2101 ctaaaatccg ccagctttgc ggaaaattca gccactttcg ccgccggaag cgccacccgc
     2161 agcagaacga atgcctgata atcactgttg ataattttgc cgtcacactg ccccagcaac
     2221 gcttcaatgc cggttaactg atgatattca cattgcaaag tatattcggt taatggcgtc
     2281 ttgcgttggg tcgttagctg gcgcagcgcc tgattcacgc cgccgccata cgctttcact
     2341 aacccaccgg tgccaagcaa tatgccgccg tagtagcgca ctaccacaga ggtaatttcc
     2401 ccgacgccgc tgcccattag ctgggcgagc atcggtttac ctgccgttcc tgccggctcc
     2461 ccgtcgtcag agaaacccag ctgttgagaa tcatccggcg cacccgccac ccacgccacg
     2521 caatggtggc gggcatcggg gtgttctgcc cgcaccgatt caacaaacgc tttcgccgcc
     2581 tcaacgccat cggtatgcgc caacatcgtt atgaagcggc tttttttgat ctcttcaacg
     2641 accgtgaccg gtgccgcagg aattaaccag ctttccatca cgccagtttc agatcccggg
     2701 tcatgttttc cacgttgttt tcgtggatca ccacgttgtc ttcgatacga atgccgccga
     2761 acggtttcag tgcttcaatt ttctgccagt tgaagtgctt gctgaactgc ccttcacgcc
     2821 acggtgccag tagcgattca atgaagtaga tacccggttc gatggttaac accatacccg
     2881 gctggagaat acgggtgcag cgcaggtacg gatattttgc cggtgccgcg aggtgtgtac
     2941 cgctatcatc ctgcataaaa ccggctacgt catgcacctg caggcccagc ggatgaccga
     3001 taccatgcgg cataaacggt ccggtgagat cgttttcgac catcgcttct tcactcatat
     3061 cggtgatgat ttgatgttta cgcagcagtt tggcgatgcg ctgatggaac tggatgtggt
     3121 aatccacata gctaacgcct gctttcatgg tggcgatcag cgccagttgt tcatcattta
     3181 cgtctttcac cagctgtgcg tagtcgttgt cgctttttgc cgaccaggta cgagtcaggt
     3241 cagccgcata gccgttatat tcggccccgg catccagcag gaagctgcgc atctcttccg
     3301 gtgcctgatg atccagtttg gtgtaatgca gcaccgaagc gtgttcgtta agcgccacaa
     3361 tgttgctgta aggtacgtcg gtatcacgat gaccggtcgc ggtcagatag gcgatgttga
     3421 tatcgaactc gctcatgcca gaacggaacg cttcttctgc cgcacgatga ccgttgaccg
     3481 ccattttctg cgcttcacgc atacaggcca gttcgtactc ggttttgaag gagcggtagt
     3541 aatgcaggta gtcgatcacc cctttcgggt tgatgttgct ggcctcaata cccagttgca
     3601 gcgcacgttc cggcaccgga ccgatataac cgatattgcc gcgcgcagcg ggcagcaggc
     3661 taccaatgcc atcggctttc ggcagtgcga tcacttccac atcttcagtc cagaaggagg
     3721 tcggcagcag ttcgacgttg tgccagtaat caaccggcag atagaaccac agtttcggct
     3781 tgttcacgcc atccaccagc aaccagcagt ttggcacctg agttaccggc acccacgctt
     3841 tgaattgcgg gttcacttta aacggatagg gatgatcgtc gagaaaaacg ttgaacagct
     3901 cgccggagtg aataagtaat gcatccagct tgaagcgcgc cagcgcatcg cgagtccgtt
     3961 cttgtaaggt agctatatga tttttataga gcgaggccag tgattccatt ttttaccctt
     4021 ctgttttttt gaccttaagt ctccgcatct tagcacatcg ttcatccaga gcgtgatttc
     4081 tgccgagcgt gatcagatcg gcatttcttt aatcttttat ttgcatattt ttaacacaaa
     4141 atacacactt cgatgcatct ggtacgacca gatcaccttg cggattcagg agactgacat
     4201 gctttacaaa ggcgacaccc tgtaccttga ctggctggaa gatggcattg ccgaactggt
     4261 atttgatgcc ccaggttcag ttaataaact cgacactgcg accgtcgcca gcctcggcga
     4321 ggccatcggc gtgctggaac agcaatcaga tctaaaaggg ctgctgctgc gttcgaacaa
     4381 agcagccttt atcgtcggtg ctgatatcac cgaatttttg tccctgttcc tcgttcctga
     4441 agaacagtta agtcagtggc tgcactttgc caatagcgtg tttaatcgcc tggaagatct
     4501 gccggtgccg accattgctg ccgtcaacgg ctatgcgctg ggtggtggct gcgaatgcgt
     4561 gctggcgacc gattatcgtc tggcgacgcc ggatctgcgc atcggtctgc cggaaaccaa
     4621 actgggcatc atgccgggct ttggcggttc tgtacgtatg ccacgtatgc tgggcgctga
     4681 cagtgcgctg gaaatcattg ccgccggtaa agatgtcggc gcggatcagg cgctgaaaat
     4741 cggtctggtg gatggggtag tcaaagcaga aaaactggtt gaaggcgcaa aggcggtttt
     4801 acgccaggcc attaacggcg acctcgactg gaaagcgaaa cgccagccga agctggaacc
     4861 gctaaaactg agcaagattg aagccaccat gagcttcacc atcgctaaag gaatggtcgc
     4921 gcaaacggcg gggaaacatt atccggcccc catcaccgca gtaaaaacca ttgaagctgc
     4981 ggcccgtttt ggtcgtgaag aagccttaaa tctggaaaac aaaagttttg tcccgctggc
     5041 gcataccaac gaagctcgcg cactggtcgg cattttcctt aacgatcaat atgtaaaagg
     5101 caaagcgaag aaactcacca aagacgttga aaccccgaaa caggctgctg tgctgggcgc
     5161 aggcatcatg ggtggcggta tcgcttacca gtccgcgtgg aaaggcgtgc cggttgtcat
     5221 gaaagatatc aacgacaagt cgttaaccct cggcatgacc gaagccgcga aactgctgaa
     5281 caagcagctt gagcgcggca agatcgatgg tctgaaactg gctggcgtga tctccacgat
     5341 ccacccaacg ctcgactacg ccggatttga ccgcgtggat attgtggtag aagcggttgt
     5401 tgaaaacccg aaagtgaaaa aagccgtact ggcagaaacc gaacaaaaag tacgccagga
     5461 taccgtgctg gcgtctaaca cttcaaccat tcctatcagc gaactggcca acgcgctgga
     5521 acgcccggaa aacttctgcg ggatgcactt ctttaacccg gtccaccgaa tgccgttggt
     5581 agaaattatt cgcggcgaga aaagctccga cgaaaccatc gcgaaagttg tcgcctgggc
     5641 gagcaagatg ggcaagacgc cgattgtggt taacgactgc cccggcttct ttgttaaccg
     5701 cgtgctgttc ccgtatttcg ccggtttcag ccagctgctg cgcgacggcg cggatttccg
     5761 caagatcgac aaagtgatgg aaaaacagtt tggctggccg atgggcccgg catatctgct
     5821 ggacgttgtg ggcattgata ccgcgcatca cgctcaggct gtcatggcag caggcttccc
     5881 gcagcggatg caaaaagatt accgcgatgc catcgacgcg ctgtttgatg ccaaccgctt
     5941 tggtcagaag aacggcctcg gtttctggcg ttataaagaa gacagcaaag gtaagccgaa
     6001 gaaagaagaa gacgccgccg ttgaagacct gctggcagaa gtgagccagc cgaagcgcga
     6061 tttcagcgaa gaagagatta tcgcccgcat gatgatcccg atggtcaacg aagtggtgcg
     6121 ctgtctggaa gagggcatta tcgccactcc ggcggaagcg gatatggcgc tggtctacgg
     6181 cctgggcttc cctccgttcc acggcggcgc gttccgctgg ctggacaccc tcggtagcgc
     6241 aaaatacctc gatatggcac agcaatatca gcacctcggc ccgctgtatg aagtgccgga
     6301 aggtctgcgt aataaagcgc gtcataacga accgtactat cctccggttg agccagcccg
     6361 tccggttggc gacctgaaaa cggcttaagg agtcacaatg gaacaggttg tcattgtcga
     6421 tgcaattcgc accccgatgg gccgttcgaa gggcggtgct tttcgtaacg tgcgtgcaga
     6481 agatctctcc gctcatttaa tgcgtagcct gctggcgcgt aacccggcgc tggaagcggc
     6541 ggccctcgac gatatttact ggggttgtgt gcagcagacg ctggagcagg gttttaatat
     6601 cgcccgtaac gcggcgctgc tggcagaagt gccgcactct gtcccggcgg ttaccgtcaa
     6661 tcgcttgtgt ggttcatcca tgcaggcact gcatgacgca gcacgaatga tcatgactgg
     6721 cgatgcgcag gcaggtctgg ttggcggcgt ggagcatatg ggccatgtgc cgatgagtca
     6781 cggcgtcgat tttcaccccg gcctgagccg caatgtcgcc aaagcggcgg gcatgatggg
     6841 cttaacggca gaaatgctgg cgcgtatgca cggtatcagc cgtgaaatgc aggatgcctt
     6901 tgccgcgcgg tcacacgccc gcgcctgggc cgccacgcag tcgggcgcat ttaaaaatga
     6961 aatcatcccg accggtggtc acgatgccga cggcgtcctg aagcagttta attacgacga
     7021 agtgattcgc ccggaaacca ccgtggaagc cctcgccacg ctgcgtccgg cgtttgatcc
     7081 agtaagcggt acggtaacgg cgggtacatc ttctgcactt tctgatggtg ccgcggccat
     7141 gctggtgatg agtgaaagcc gcgcccgtga attaggtctt aagccgcgcg cccgtgtgcg
     7201 ttcgatggcg gtcgttggtt gtgatccgtc gattatgggt tacggcccgg ttcctgcctc
     7261 gaagctggcg ctgaaaaaag cggggctttc tgccagcgat atcggcgtgt ttgagatgaa
     7321 cgaagccttt gccgcgcaga tcctgccgtg cattaaagat ctcgggctaa tggagcagat
     7381 tgacgagaag atcaacctca atggtggtgc gatcgcgctg ggtcatccgc tgggttgttc
     7441 cggtgcgcgt atcagcacca cgttgctgaa tctgatggaa cacaaagacg ttcagtttgg
     7501 tctggcgacg atgtgtatcg gtctgggtca gggtattgcg acggtgtttg agcgggttta
     7561 accacttgcc ggatgcggcg tgaacgcctt atccggtcta ccgatcctgc accaatgtag
     7621 gcctgataag acgcgccagc gtcgcatcag gcatcgtgca ccgattgccg gatgcgacat
     7681 agatctaaat gccactgaat aaagttttag ttgccgttct tcccgcctgt caggggcggg
     7741 tttttatatc tcagataaat gcaaacgcat cgccaaacag acgatcttcc cgcgcattac
     7801 gctcactgca aaacaggtca cgagcaattt tcgccatctc aaaacgtccg gcaatataga
     7861 tatcatgctc tgccagcgta ccatgatcct gcaagaccgc cgttaacacg gtgccagtac
     7921 gcccacgcca gcccgcttcc ggttgttcaa ccaccggcac cacttgcaga ccaggatgct
     7981 tcagcgaaag cgcctcaagc tcgcagagat catacagatg ttgctcttca cgcccacccc
     8041 agtaaatggt gatatcgcga ttgggattac gcgccaacgc cgtcaataaa atcgagcgcg
     8101 cataggagaa cccggtgccg cccgcaatca aaatcatcgg acgctcttca tcatcgcgca
     8161 gccacgcttc tccgtgggga atatcgacca cgatttgatg atctttgagg atgcggtcca
     8221 tgactgcttt cgcgtaaagg ttgatttcag aagcgccaat atgcagctcg ataaaccctt
     8281 tttcatccgg cgtcgaagcc attgaaaacg gacgtttgtc gcgctcatcc atcactacca
     8341 tcaaatactg accagcacga aaagaaaagg ccgcgtctgg tacgattcgg acacggtata
     8401 cagtatccgt gatagcttct accgaggtca ctttacagct taaggttgtc atgcgctttc
     8461 tctgtcggat caaataaata gggcaaaact aacgcgcatc aggcgctttt accgttgtta
     8521 aaaatagcca gttcatccca gatggcgtca atatgcgcga cgacatcagg atcttttttg
     8581 atgggacgtc cccattcacg ctgggtttcc cccggccatt tattcgtggc atccagcccc
     8641 atttttgaac ccagcccgga gacaggcgag gcaaaatcca gataatcaat aggcgtattt
     8701 tctaccagaa cagtatcccg cgccgggtcc atacgggtgg taatcgccca aatcacatcg
     8761 ttccagtcgc gtgcgttgac gtcatcatcg caaacgatca caaatttagt gtacataaac
     8821 tggcgtaaga acgaccagac gcccatcatg acgcgcttcg cgtgtccggc gtactgtttt
     8881 ttgattgtca ctaccgccag acgataagaa cagccttccg gtggcaggta aaaatcgaca
     8941 atttccggga actgtttttg cagaatcggc acaaacactt cgttcagtgc gacacccagc
     9001 accgcgggtt catccggcgg acgaccagta taggtggagt ggtaaatcgc atcttcacgc
     9061 tgggtaatat gcgtcacggt aaataccggg aaactatcga cttcgttata gtaaccggtg
     9121 tggtcgccat acggcccttc cggcgcagtt tcgccttgtt cgatataccc ttccagcaca
     9181 atctccgcac tggcgggcac ttcaaggtca ttggagatac acttcaccac ttcggtcttg
     9241 gtaccgcgca gcaatccggc aaacgcatac tctgaaagtg tatccggaac gggggtgact
     9301 gcaccgagaa tcgtggcggg atcggcaccc agcgccacag aaaccgggaa acgttcgccc
     9361 ggatgcgccg cacaccactc ctgataatcc agcgcgccgc cgcgatgcga cagccagcgc
     9421 ataatcagtt tgtttttacc aatcagctgc tggcgataaa tgcccagatt ctgccgctct
     9481 ttatgcgggc cgcgcgttac ggtcagcccc caggtaatca gcggcgcggc atcttccggc
     9541 cagcaggtca taatgggaat gcgattgaga tcgacgtcat cgccagagac gattttttgt
     9601 tggcagggcg caccacgcag ccgctttgtc ggcatgttca atacttgctt aaactgcggc
     9661 agtttatcaa acaggtcgcg gaaacctttt ggcggctcgg gctctttcag aaacgccaat
     9721 aatttaccaa cttcacgcag cgccgaaaca tcttcctgcc ccatgcccat cgccacgcgc
     9781 tttggcgtac cgaacaggtt gcacagcacc ggcattgagt agcctttagg gttttcgaac
     9841 aacagcgcag gcccaccggc acgcaaagtg cggtcagcaa tttcagtgat ttccagatgc
     9901 ggatccaccg ggagcgtgat acgttttagc tcaccctgct gttcaagcag cgtcaagaag
     9961 tcgcgtaaat cgttatattt catggcgtcc attgtagcct cttaatctac gcccattata
    10021 cggcgttcat ctttgcgatg ctgtaaattt gttaaattag cgtgaactct gacggtataa
    10081 cgcaaaccgg ggaatataat taacttagcg taaagctttt gctatccttg cgccccgatt
    10141 aaacggataa gagtcattat gcaatcttgg tatttactgt actgcaagcg cgggcaactt
    10201 caacgtgccc aggaacacct tgagagacag gctgtgaatt gcctggcacc gatgatcacc
    10261 ctggaaaaaa tcgtgcgtgg aaaacgtact gcagtcagtg agccattgtt tcccaactac
    10321 ctgtttgtcg aatttgatcc agaagtgatt cataccacga ctatcaacgc gacccgcggt
    10381 gtcagccact tcgtgcgctt tggcgcgtcg ctagcgatag tcccatcggc ggtgattcat
    10441 cagctatcgg tatataaacc gaaagacatt gtcgatccgg caacccctta tccgggagat
    10501 aaggtgatta ttaccgaagg cgcgttcgaa ggctttcagg ccattttcac cgaacccgat
    10561 ggtgaggctc gctccatgct attgcttaat cttattaata aagagattaa gcacagtgtg
    10621 aagaacaccg agttccgcaa actctaaaac gcaatcccaa accgtgtttt gacattagca
    10681 tccgtggtgg cagccagcca tgcggcatct tctccacgcc agtgcgcaat acgttgcaaa
    10741 atatggggca gatgggctgg ctcgttgcgc cgggatgatg gctttggcgt gagatcgcga
    10801 gggagcagat acggcgcatc agtttcgatc agtaattttt ccgccggaat caacggcaaa
    10861 agttcccgca gctccagtcc gcgtcgttca tcgtaaaccc aaccggtaat gccgatataa
    10921 attccatgcg ccacgcacgc ctgcatctct tcgcgtgtgc cggtaaagca atgaagaacc
    10981 gcaccaggca gtttatccag ccacggctcc agcaatgtca taaaccgctc gtgggcatcg
    11041 cgacagtgca taaataccgg catgtttaaa tctgcggcaa tgcgtagctg ggcaacaaaa
    11101 gcgcgttcct gctcttccgg cgtcgaaaag ttgcggttaa agtcgagacc acattcacca
    11161 atcgccacca cttctggctg cgcggccagc tcaataatcg cttcttcagt cgcagcttgc
    11221 cactggctgc tgtcgtgagg atgtacgccc gccgttgacc aacaggacga atactgacgc
    11281 gccagctttt gcgcctgctg gctttcacgc aggttagtgc cggtgatgag tagcccatta
    11341 actcccgcgt caaaagcgcg cgctacaaca tcatcacggt ctttcgcaaa ttgcgaactg
    11401 gtcaaattaa cgccgatatc aaacatcctg tactccatat gacaaccgcc ctgacgggcg
    11461 gttgaattta ttcttcagtt ttttcgcttt ctgcttcagc gtcgttttcc tcttcccgat
    11521 ttcgcccttt accaacgtaa aagcgtgaga agaagacacc gatttcaaac agacagtaca
    11581 tcgggatcgc caatagcgtt tgcgagaaga catccggcgg cgtcagcaac atcccgacaa
    11641 cgaatgcacc aaccagcaca tacgggcgtt ttttgcgtaa gtcttctggc gaggtaatcc
    11701 ccatccagca cagcagcaca attgccaccg gcacttcaaa ggagacacca aacgccataa
    11761 acagcgccat aacgaagctt aaatagctgg cgatgtcggt ggatacctgc accccttccg
    11821 gcgcggtatt ggcaaggaag ccaaatgcca gcggaaagac cacaaagtag gcgaatgcca
    11881 tgccgatata aaacagcaga gagctggaaa ccagcagcgg caccaccagg cgacgttcat
    11941 gcttatacag cgctggggcg ataaatgccc acacctgata gagaatcacc ggcgctgaca
    12001 gaatcagcga caccataaag gtcagcttga tcggcgtaaa gaacggcgag gccacgtcgg
    12061 tggcgatcat cgttgaacct tgcggcaact gcttgatcaa tggcgcggat accaggtgat
    12121 agatgtcatt ggcgaaatag accagacaca ggaatatcac gatcaccgcg ataatgcagt
    12181 tcagcagacg cttacgcagc tcaattagat gcgtgataag cggttgagta tcttctacag
    12241 acatgtttac ggtttatcac tcgacgaagg ggaaggtgca gcggttttcg gttcagcgtc
    12301 cgcagcaggt tttaccaccg gctctggcgt ggtttctggc ttctgttccg gcgaactggc
    12361 ctgcgtttgt gcagcggcag gcgttacgcc ctcatgcgca gcttcattat ctttcaccac
    12421 cgggttatgg atggtgtgcg cttcatcgct cgccttttca ggatcgtttg caacgtagga
    12481 acgcttcatc gactccgcgg cctggcgtag ttcatccatc gacgctttca gttcgggcgt
    12541 caggttagtg aggctcgcct tttcaacctt tttcagactg tcctgaaact cctggagttt
    12601 taactcctgg gtcagttcgt tctgcaccgt tgtcgccagt gaacgcaacg cgcgaatcca
    12661 gcccgctacc gtttttaccg ccacaggcag tcgttgcggc cccagaacga cgaggccgat
    12721 gatgaacacc aatagcagtt cgctaaaacc gatatcaaac acggattaca cctgctcttt
    12781 atcgtggcgc ttcgcgtctt ctgttttagc ctgttcctga ttcgtatccg cctgcttatc
    12841 ggcgatagtt ttcgcagtaa aatcagcatc ctgactggtt ttatcctgct ttggttcatc
    12901 atcgctcatt gcttttttaa agcctttgat cgacgcacca agatcggaac cgatggagcc
    12961 gagctttttg gtgccaaaaa gcagtacaac gatgacggca ataatcaata actgccaaat
    13021 actgatacca cccatacatg ttcctctgtg gtagatgatg attaaacaag gccgcattat
    13081 acgttacacg acccgccttg agcgatgaaa aaatcagcgt gttttgcgcc aaccgacaaa
    13141 ccaggcgatc agaccacctg ccattaacca gccgggcatc agcccccatt caggtcggct
    13201 gaccaacaag aatgtgccac ttaatactaa cgtagcgcca attccgagaa aataacgcga
    13261 ttgtccctga cgtacatgat ttgactgaag ctcgcgggca atcttatcaa cactgtgctg
    13321 taaatacttg ccctggcgca aactgtcgta aaccaattca ggcagttctg gcattttttc
    13381 gacccagaac ggcgcttttt ctttaaatgc tctcaccagg gcaggaatac cgacctgatc
    13441 tttaatccac gactccagga aaggcttcgc tgttttccat aaatcgagct gcggataaag
    13501 ctggcgtcct accccttcga catagagcag ggttttctgg agtaacacca gttgcggctg
    13561 cacttccata ttgaagcgac gcgccgtatt aaacagattt aacagtacat gtccaaacga
    13621 aatttcggcc agcggtttct caaagatagg ttcacagacc gtacgaatgg caaattcgaa
    13681 ctcttcaacg ttggtatctg gtggcaccca gccagaatcg acgtgtagct ctgccacttt
    13741 gcgatagtcg cgattaaaga aggcgataaa gttttctgcc agatagcgtt tatcttcttt
    13801 gtttagcgag ccaacaatcc cgcaatcaat gccgatatat ttcgggtttt ccgggtgttc
    13861 atagcttacg aaaatgttgc cagggtgcat atcggcatgg aaaaagctgt cgcgaaagac
    13921 ctgagtgaag aacacctgca cgccacgttc cgccagcaat ttcatgttgg tgccattttt
    13981 ctccagcgtc gcaacatcag acaccggaat gccgtaaatg cgctccatca ccatcatccc
    14041 ttcactacaa tagtcagggt aaacttccgg gatgtagagc atcgggctgt cttcaaaatt
    14101 tcgccgaagc tgaatggcgt tggcagattc ccgcagcaaa ttcagttcat caatcaaggt
    14161 cttttcgtac tcgcgcacca cttcggttgg gcgcagacgg cgaccatccg gcagcaaacg
    14221 cggcacccag cgagccagac ggtagataag tttcagatcc gctttaataa ccggcaaaat
    14281 atccgggcgg atgactttaa tcaccacctc tttaccattc gatttcaatc gcgcggtatg
    14341 aacctgggcg atagaagcag aagccagtgg cttgatttca aaatcgtcaa accacgcttc
    14401 taccggcaag ccgcccattg cagcttcaat ctgctgcttc gccagcttgc catcaaacgg
    14461 agcaactttg tcctgcaata acgccagctg atcggcaata tgcggtggaa aaagatcgcg
    14521 gcgggttgat aacatttgcc cgaacttgat ccaaaccggc cccagttctt gcagggccag
    14581 tcgtagtcgc tcacctaaaa gtttgtcttt atgccgattt ggcatccaga ataatgagta
    14641 tcgccatagc cgtagcggca gggtgatacg cattttgggg atcagttcat caagtccgta
    14701 gcttaaaaaa gtgcgaatga tgaaatatag gcgccgtact tcacctggcg tcatttagcc
    14761 tccagttttt ccagccgttt ggtcagggca tcaacagcac gctcgacggc agccgtctct
    14821 tccgcaaacc aggccacttc aagcggaccg ggtgccatac gccactcttc agtaatggct
    14881 tccgccacat aacgttgctg gcgcttaatg ccgtgatgca ggaactttgc gcctccgcgc
    14941 atggctttgc tgattccttc agcggcgata tcaccggtat aaggggccag cagttccgca
    15001 gggtcgaact ctgccagatc tgccagcgca acgaagtttt gcaccacctg aatatcgccc
    15061 tgcacttcca gctcaccact gcgaatcagt gcggtaagct gctggcgatc gcgaagtttc
    15121 ggcaacacac tggcgtaggc gataacggtg cagtcagcat cgcctgccca ttcgcccagt
    15181 acatcaacct ggcgttcgct gaacaccaga atcaatgacg tcgaaaagcc ttttacctcc
    15241 acgcgcaata ctttacccag cagacgcgag cgggctgttt tcagcgcggg tgagcgatac
    15301 aggaaggtgt tgagcagact ttcaattcct gccgtcacta aaggtttaaa aggcatttcc
    15361 ggtctcctgt cagaacttat aaccacgatg cagcgccaca acccctgccg tcagattgta
    15421 gtagtcgaca ctttcgaatc cggcatcctg catcatggct ttcagggtat cctgatcggg
    15481 atgcatacgg atggattctg ccagataacg gtagctgtcg gcgtcgttcg cgaccagtga
    15541 gccaatacgc ggcagcacat ggaaggagta tgcatcatag gctttgctca gcggctcgat
    15601 aattggcttc gagaactcaa gcaccagcag gcggccgccg ggtttcagca cgcgatacat
    15661 tgaacgcagt gctttatctt tgtcggtgac gttacgcaga ccaaacgaaa tggtgatgca
    15721 atcaaaggtg ttatccggga acggcagcgc ctcagcgttc gcctgaacat actcaacgtt
    15781 gccaatcaca ccgatattac gcagcttctc gcgtcccatt ttgagcatgg attcattgat
    15841 atcagcaagg accactttgc cagtttctcc gaccaggcgg gagaatttcg ctgtcaggtc
    15901 gccggtgcca ccagccagat ccagcacggt ctgcccacgg cgtacgccgc tgcaatcaat
    15961 cgtgaatcgc ttccacaaac gatgaatacc aaatgacatc aaatcattca tgacatcgta
    16021 ttttgatgcc acggaatgga aaacgtgggc gaccatatcc gctttttgtt ccttcgcgac
    16081 ggtctgaaaa ccaaagtgcg tcgtttcttg tgacttatcc accatctcaa tgcctgctca
    16141 tcaaaaaatt gttccagaag tgtaacagat tgggcgtcga tgccctagat ttctacccgg
    16201 cttaactacc cctaatgggc tagcgcgact gctgattata ttcatcatcg cgttgataag
    16261 cttcatcatt cggctgctcc ggaaccgacc gaagtcgata ctcttcattc tggctcaccg
    16321 cctgttcagc caaatccgga ttaatctcgc gtttaatttc tactcctaaa ccgcgaaacg
    16381 cttctgcctg cgccagcaca tttccgcgac ctgaagagag ttttttcatt gcctggcggt
    16441 agttatcctg cgctttgtcg agactttgac caatcgcgga catgtcatcg atgaacaaac
    16501 gcatcttgtc gtacagcttg ctggcacgat cggcgatttg ctgggcgttg cggctttgat
    16561 gctcataacg ccacaggttg gcaatagtac gcaacgccac cagcagcgta gtcgggctaa
    16621 ccagcatgat attgtttttc aacgcttcgg tgatcagctc cggctggcgg tcaagcgcca
    16681 gtaaaaaagc gggttcaacg ggaataaaca tcagcacgta atccagagtt cgcagccccg
    16741 gcagctgttg ataatctttg cgtcccagca aacggatatg gttacgcacc gacgcgatat
    16801 gttcctgtag cgcgctttcg cgggtgtagt cgtcttcggc gttaaaatag cgttcatagg
    16861 cgaccagcgt cattttggcg tcgatcacca catcttttcc ctgcggcagc cgcacgatga
    16921 catccggctg catccgcgag cgggcgtcat tttcgatgct gacctgggtt tcatattcat
    16981 acccttcacg cagaccggaa gcctccagca cccgcgtcaa tactacctcg ccccagttgc
    17041 cctgggtttt attgtcgcct ttcagcgcgc gcgtcaggtt gatcgcttcc tgggccattt
    17101 gcgcgttgag ttgctggaga ttgcgaattt cgtgggtcag ggtatggcgt tcttgtgctt
    17161 ctttaccgaa gctgtcctga acctgacggc ggaaaccgtc cagttgttca cgtagcggcg
    17221 acaacaggct gttcagactc tgacggtttt gctcatcaac ccggcgattg ctgtgctcaa
    17281 aaatacggtt ggcgaggttt tcaaactgct cactgaggcg ctgctcgctg ttaatcatct
    17341 ggcgaatttt atcgtcagca tgttgctgtg cggcttccat ccgcgtggtt acttcacgca
    17401 gatcggcctc cagagaggtg ttaatacttt gcaggctgcg cacttcgtta ttgagtaact
    17461 cgcactctgc acgccagtgc tcgctttggg taatttgttg ttttgccgcg cttaactccg
    17521 cgaccatctc ttcacgttca gctaattgct cggctttttg ctgcgcatgt tgataactgg
    17581 caaacagcca gccaattgcc acacccacca acgcaataac tgcgtaaacc atgattgaga
    17641 aatccacaac gcctcctgac gcaacaactt acccgcacaa aatagaattg tgctgtacaa
    17701 acgtccagtt ggaaaggcat ttcctgcgag gcgctacgca aaaatacaaa aggccgaacg
    17761 cgtcggcctt cagacaggag aagagaatta cagcagacga cgcgccgctt ccaccacgat
    17821 tttcaccgct tggctttcag tttgtttcat cgtctcagca ttcgggatct cttgctgggt
    17881 gcggttaacg ataacacccg ctaccatacc ggcacgcagg ccctgacttg cacacatggt
    17941 cagcagggtt gcagattcca tttcatagtt cattatgccc atcgcctgcc actcttccat
    18001 agaaccttta aagtgacgaa ctacgcgacc agagtaagta tcgtaacgtt cctgacctgg
    18061 gtagaaggta tcagaagaag ctgtcacgcc aacgtgagtt gtcgcgccaa tggatttcgc
    18121 agcttcaacc agcgcagtcg tacattcgaa atcagcgaca gccgggaatt ccagcggtgc
    18181 gaagtgcagg ctcgcgccat ccagacggac agacgccgtg gtaaccagga catcacccac
    18241 attaatatgc ggctgaatag cgcccgttgt accgatacgc aggaaggtgc gaatgcccag
    18301 ctgtgccagc tcttcaacag caatagaggt agacgggccg ccgataccgg tagagcagac
    18361 gataacaggt ttaccatcca gctctgcacg ccaggtagtg aattcgcggt gagatgccag
    18421 cttaaccggc ttatccatca gcgcggcgat cttttccaca cgatccgggt cgccagggac
    18481 gatggcaagc gtagcccctt gtaaatcgtt tttagtgagg ccgagatgaa aaacatcaga
    18541 cttggacata tacaactcct ctgtgaatcg gtttagtcag aagaagcaaa aagacacttt
    18601 accgaagggt ttaacatttt ttcgtgatac tcatcaccat gacgcaaatg cgttgcataa
    18661 atttacccac caaaagtgat tcaaatcaca taaatcactc ttcattctgc aggactgcat
    18721 aaaagatgag ccgattcagg caatgtgatt tgttgcatgc tgactatagt taacattgcg
    18781 caacattttt ataagggtac ggagaatacc atggcaacaa cacaacaatc tggatttgca
    18841 cctgctgcat cgcctctcgc ttcgaccatc gttcagaccc tggacgacgc gattgtggcg
    18901 ggcttcacct ctatcccttc acaaggggat aacatgcctg cttaccatgc cagaccaaag
    18961 caaagcgatg gcccactgcc agtggtcatt gtagtgcagg aaatttttgg cgtgcatgaa
    19021 catatccgcg acatttgtcg ccgtctggcg ctggaggggt atctggctat cgcacctgaa
    19081 ctttacttcc gcgaaggcga tccgaatgat tttgccgata tccccacgtt gcttagcggt
    19141 ctggtagcaa aagtgcctga ctcgcaggtg ctggccgatc tcgatcatgc cgccagttgg
    19201 gcttcccgca acggcggcga tgttcatcgt ttaatgatca ccggattctg ctggggtgga
    19261 cgtatcacct ggctgtatgc cgcgcataat ccacagctaa aagctgcagt ggcgtggtac
    19321 ggcaaactga cgggcgacaa gtcgctgaat tcaccgaaac aacctgttga tatcgcaacc
    19381 gatcttaacg cgccgattct cggcttatat ggcggtcagg ataacagcat tccgcaggag
    19441 agcgttgaaa ccatgcgcca ggcgctgcgg gctgctaatg cgaaagcaga gattatcgtg
    19501 tacccggatg ccgggcatgc attcaacgct gattatcgcc cgagctatca tgccgaatct
    19561 gcgaaagatg gctggcagcg tatgctggaa tggtttaagc agtatggaga gaagcggagt
    19621 tagtttcctc ttagtctata tttgagatgg tgagtaaacg agtgcgtttt gctgccatct
    19681 ctgtccccca aaaaataatc agtatgaatt gattggagat tacgactccg ctatttgacc
    19741 ttaagaaagt cggcaagtca tagtcataaa tgagtctctt caatggaaga caccttagag
    19801 atttaccatc aactcccatt cacaaacaaa cattaattat tatcaacaca ttcacattaa
    19861 ttataaatca tctcagacct tcctgataaa aagatattaa agtgaaacat tttgtttttc
    19921 acattttagt tcatcacttt taggcgtaaa taaaggtaaa aaaaacaaca atactatttc
    19981 tctttcacaa aagaattaac aaaaggtttt tcaaggatga gtgatattat ttacctgacc
    20041 ataaccggag aacaacaagg atgtatttcc tctcgttgtg gaacgagcgc atcaataggt
    20101 aatcgctggc agattggcca tgatgatgaa atcttcgcgt tctcgctctc taatagtatt
    20161 acgaatacag gcaaagggtc ataattgcat ggccttagtt tctgtaaatt gattgacaaa
    20221 agctccccgt tacttattaa cgctattaac aataacgagc agttgttcat ggagtttgac
    20281 ttctatcgga tcaataggtt tggtcgatgg gagaaatatt acaacattca actccgcggc
    20341 gcgctcttat cagcaatcaa tcacctgttt actgagaaca atctggatac agaagccatc
    20401 actgtcagtt attaatatat actctccagg catctgattg ctaatactga atttagttat
    20461 tgggcatttc ccgataacta caaccgtctg tttataccac ggccaaaaac taaagctggt
    20521 aatggtttca aaacacttaa tagtaaagcg gttggcagac tgcttgctgc tgtcggcata
    20581 tacaatggga atatcgaagg attcagggaa acagcagaca agttgggggg agatgcacct
    20641 gctggttatg atcaatttat ggataacaaa gggttactca tcgctggtgc ttcaatagtt
    20701 gctggagcag ttctggggag aatgagactc cctgatttgg aatcacttga gcatttcgga
    20761 gccagaggag cagtatcagg aagaccattt aatcccgagc ttgccggtgg tccaatagaa
    20821 aacctgacga cagatggcgt aacaatcaac agggaaggta tcgctattgt tgaaaaacat
    20881 atagccagat ttggtcatga tcctgtaaat gaagtgatga tcaatagact gaaagacatt
    20941 gagaaaggaa aaatcccccc agaacaagta gatcttaact tctatactca tgagtgtcgg
    21001 gaatatcagc gttactgtaa tcttggttgg gaaacaggac aacctgatgg tgacgcaggc
    21061 tatgcactct ggaatcat
//
LOCUS       DAJTVE010000071        20763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000071 DAJTVE010000000
VERSION     DAJTVE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            112..1332
                     /gene="coaBC"
                     /locus_tag="OO439_002458"
     CDS             112..1332
                     /gene="coaBC"
                     /locus_tag="OO439_002458"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6316804.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1310..1768
                     /gene="dut"
                     /locus_tag="OO439_002459"
     CDS             1310..1768
                     /gene="dut"
                     /locus_tag="OO439_002459"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6316805.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            1875..2471
                     /gene="slmA"
                     /locus_tag="OO439_002460"
     CDS             1875..2471
                     /gene="slmA"
                     /locus_tag="OO439_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6316806.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2508..3149)
                     /gene="pyrE"
                     /locus_tag="OO439_002461"
     CDS             complement(2508..3149)
                     /gene="pyrE"
                     /locus_tag="OO439_002461"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6316807.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3215..3931)
                     /gene="rph"
                     /locus_tag="OO439_002462"
     CDS             complement(3215..3931)
                     /gene="rph"
                     /locus_tag="OO439_002462"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6316808.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4058..4921
                     /locus_tag="OO439_002463"
     CDS             4058..4921
                     /locus_tag="OO439_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6316809.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5143..5967
                     /gene="dinD"
                     /locus_tag="OO439_002464"
     CDS             5143..5967
                     /gene="dinD"
                     /locus_tag="OO439_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6316810.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6259..6876
                     /locus_tag="OO439_002465"
     CDS             6259..6876
                     /locus_tag="OO439_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6316811.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(6873..8555)
                     /gene="ligB"
                     /locus_tag="OO439_002466"
     CDS             complement(6873..8555)
                     /gene="ligB"
                     /locus_tag="OO439_002466"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6316812.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            8813..9436
                     /gene="gmk"
                     /locus_tag="OO439_002467"
     CDS             8813..9436
                     /gene="gmk"
                     /locus_tag="OO439_002467"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6316813.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9491..9766
                     /gene="rpoZ"
                     /locus_tag="OO439_002468"
     CDS             9491..9766
                     /gene="rpoZ"
                     /locus_tag="OO439_002468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6316814.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            9785..11893
                     /gene="spoT"
                     /locus_tag="OO439_002469"
     CDS             9785..11893
                     /gene="spoT"
                     /locus_tag="OO439_002469"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6316815.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            11900..12589
                     /gene="trmH"
                     /locus_tag="OO439_002470"
     CDS             11900..12589
                     /gene="trmH"
                     /locus_tag="OO439_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6316816.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12595..14676
                     /gene="recG"
                     /locus_tag="OO439_002471"
     CDS             12595..14676
                     /gene="recG"
                     /locus_tag="OO439_002471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6316817.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14845..16050)
                     /gene="gltS"
                     /locus_tag="OO439_002472"
     CDS             complement(14845..16050)
                     /gene="gltS"
                     /locus_tag="OO439_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6316818.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16330..17721
                     /gene="xanP"
                     /locus_tag="OO439_002473"
     CDS             16330..17721
                     /gene="xanP"
                     /locus_tag="OO439_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6316819.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17842..19551
                     /locus_tag="OO439_002474"
     CDS             17842..19551
                     /locus_tag="OO439_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6316820.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19603..>20763)
                     /gene="yicI"
                     /locus_tag="OO439_002475"
     CDS             complement(19603..>20763)
                     /gene="yicI"
                     /locus_tag="OO439_002475"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6316821.1"
                     /translation="YDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDG
                     SDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYE
                     SMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYR
                     VPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPACDYL
                     DRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSLPVY
                     VRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKATRTG
                     NTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5026 a         5643 c         5337 g         4757 t
ORIGIN      
        1 ttttcaatcc tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag
       61 tattgtgata aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg
      121 gccggtaaaa aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa
      181 ctggtgcgtc gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca
      241 aaagccttta tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt
      301 ctgctggacc cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat
      361 ttagtgattc tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg
      421 aatgacctgg tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc
      481 atgaaccagc agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc
      541 cgtggtttgc tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct
      601 gggcgaatgc tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc
      661 aacgacctga aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat
      721 ccggtgcgtt atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc
      781 gctgcccgtc gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca
      841 ccgtttgtta aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct
      901 tctgtacagc agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct
      961 accgtggccc cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa
     1021 atggttaaaa accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac
     1081 gtcgttggat ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc
     1141 cgtaaaaacc ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac
     1201 agcgacaaca acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag
     1261 cgcaaagagc tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa
     1321 aatcgacgtt aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc
     1381 cacctctggc tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc
     1441 tccgggtgac tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact
     1501 ggcggcaatg atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa
     1561 cctggtagga ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg
     1621 tggtcaggac agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc
     1681 ggtagtacag gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg
     1741 cggctttggt cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag
     1801 ccgcaaacat ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag
     1861 gggtattttg taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac
     1921 ttcagtctct ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa
     1981 aactggccgc ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga
     2041 cccgcatgtt cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc
     2101 tgattctgaa agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc
     2161 tcggttttgg tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt
     2221 ttgaacagga tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc
     2281 tgcgccaggt attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa
     2341 ccctgctggc aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca
     2401 gcgaatttaa ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc
     2461 agttgcagta atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac
     2521 tcttcgcgat aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg
     2581 taagcaatca ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca
     2641 acttcctgaa tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac
     2701 acgccagcaa gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt
     2761 ccggcggtga tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga
     2821 ttgccgcctt caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg
     2881 tcatgatgct ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca
     2941 gggccaaaca gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg
     3001 cctaacagtg ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt
     3061 ttacgcccgg atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat
     3121 tcaataaact ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga
     3181 caaaaaaaag gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg
     3241 tcgctacaat ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt
     3301 gggtgaacgg ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca
     3361 tcactacgtt catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg
     3421 cttcgccgtt cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt
     3481 tcagcttacc gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg
     3541 taatcgaagc ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg
     3601 tgaactcacc cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct
     3661 ggatttccat tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt
     3721 gggtagaacg cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca
     3781 ggaagcgcgg cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt
     3841 cgaccagcac cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca
     3901 cctgattatt gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg
     3961 gctgcgcatt atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat
     4021 aatccttcca cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta
     4081 cgcccggcgt gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa
     4141 ccagcgttat ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt
     4201 cgttcgcgag cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta
     4261 tgagccagat gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct
     4321 ggtaactgcc gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga
     4381 tattctgcgc tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc
     4441 tgaaattctc gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga
     4501 aggtcaggcg ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt
     4561 caaagtccgc gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa
     4621 gctggaagat gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct
     4681 ggcacaacga attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac
     4741 ctacaacatt ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga
     4801 gttcaaccgc gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc
     4861 tgccatcgag ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta
     4921 actcgtaaca ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg
     4981 gcatttttct tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat
     5041 gtttttttgc gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta
     5101 tagacttact ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa
     5161 ccttttgaag aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa
     5221 ttggcaccgt tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg
     5281 actcaagcgt gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa
     5341 atggttgtat taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat
     5401 gcctgttacc tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa
     5461 acttattttg ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa
     5521 cttcgtgaag acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa
     5581 ttagttgagg cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag
     5641 aatcatggtt accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag
     5701 gggctgaaaa agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat
     5761 ctctttcgag ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag
     5821 caagctaata ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt
     5881 ggcggaacca tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac
     5941 agtgtaaaaa ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat
     6001 acccgatgcg taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt
     6061 cttttccacc cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca
     6121 gaagaatgca tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc
     6181 ctctcacctt aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag
     6241 atttttaggg agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa
     6301 gccatgacgg gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt
     6361 attgcgaccg ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat
     6421 ccgctcggtt gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc
     6481 accactatcg ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg
     6541 ctcggactgg tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc
     6601 ccaattattg ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat
     6661 atgttctgta aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt
     6721 gcctccgccg tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt
     6781 atctccaccc tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga
     6841 ttaccggtct tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg
     6901 gcagccagcc aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt
     6961 ctggcgcgcc cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa
     7021 agttgcgacc aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc
     7081 attgccatca cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac
     7141 tgcgcacttt tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa
     7201 agccaggaaa agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca
     7261 ccaatgccat ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac
     7321 tgttcctgac aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt
     7381 ggtgtcggtt ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc
     7441 tgaccggcaa ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc
     7501 ctgacggaac caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt
     7561 gacgcaacca ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg
     7621 gcaacctgag ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc
     7681 caatggcggg attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt
     7741 aatttcgctt tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca
     7801 cgggtatacc tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc
     7861 attaactgcg gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg
     7921 ctattcccct ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt
     7981 tgttgttgga tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct
     8041 aaaggcccgc taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc
     8101 tcgcctttca ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc
     8161 agggttaccg caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc
     8221 cacagactta atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc
     8281 ataaccgcac catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt
     8341 tgccactgcg taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc
     8401 ccttccttcc agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct
     8461 tcctgtgctc tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac
     8521 aagatactta ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag
     8581 gcagatatac aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc
     8641 ttcctgaaaa tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa
     8701 acgcaacaac tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta
     8761 taataagctc gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca
     8821 aggcacgctt tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc
     8881 tttattaaaa acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca
     8941 accgcgtcct ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa
     9001 agaaatgatt agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg
     9061 cacttcgcgt gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat
     9121 cgactggcag ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat
     9181 tttaccgccg tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga
     9241 agaggtcatt gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata
     9301 tgattatctg attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat
     9361 tcgcgccgaa cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa
     9421 attgttggca gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag
     9481 ctttttaagt atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt
     9541 tgacctggta ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc
     9601 gctggtaccg gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg
     9661 tctgatcaac aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc
     9721 cgctgaatta caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc
     9781 gcccttgtat ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca
     9841 aatcaagcgt ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg
     9901 ttcaagcggt gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat
     9961 gaaactcgac tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc
    10021 cgccacctac caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg
    10081 ggtatcgaaa cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt
    10141 tcgcaagatg attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga
    10201 ccgtacccac aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc
    10261 ccgtgaaact ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa
    10321 aaccgaactc gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa
    10381 agaagtggtg aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga
    10441 aatcgaaggg cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca
    10501 tctttattcg atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga
    10561 catctacgct ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca
    10621 aatgcatagc ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa
    10681 agcgaacggc tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga
    10741 ggtccagatc cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg
    10801 ggcttataaa gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat
    10861 gcaaagcttg ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt
    10921 taaatccgat ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga
    10981 gctgcctgcc ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca
    11041 tgcctgcgtg ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag
    11101 cggtcaaacc gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa
    11161 ctttgtcgtt agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg
    11221 tgatgattct gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa
    11281 gctgaatgaa atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac
    11341 gcttgacgat ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa
    11401 aaatctgcaa catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc
    11461 cattaaaggt gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg
    11521 cgacccgatt atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg
    11581 ccgtaatatc cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa
    11641 agagacggcg caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc
    11701 gctggcaaac ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac
    11761 ggaagagaaa gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt
    11821 gcatctggcg aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg
    11881 aaaccgaaat taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc
    11941 caggcggcag cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc
    12001 tgcgattatt cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg
    12061 tagccgcatg cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa
    12121 aacacaccgc accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct
    12181 ggcaacccat ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac
    12241 ctgcattttg atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga
    12301 tcaggacatc atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc
    12361 agccctcatt ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga
    12421 aaacagcatg ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt
    12481 gctggcgaaa gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga
    12541 gatcgaagct gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa
    12601 ggtcgcctgt tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt
    12661 aacaaactgg cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg
    12721 cgctacgaag atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc
    12781 acggtggaag gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc
    12841 tgccagatca gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca
    12901 atgaaaaata gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt
    12961 aaatatggtg cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca
    13021 gaattacagg aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg
    13081 ctgcgtaaat taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc
    13141 ctgccgccgg aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac
    13201 cgcccgccac cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt
    13261 cgtctgattc tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga
    13321 gcacagcgtt ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc
    13381 gcagccttac cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc
    13441 gatatggcgc tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa
    13501 acgctggtcg ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg
    13561 atggcaccaa ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa
    13621 ccgctcggta tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct
    13681 cagcaggaag ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc
    13741 caggaacagg tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt
    13801 ggcgtgcatc agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat
    13861 cagttgatca tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat
    13921 ctcgatacct cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct
    13981 attcctgata ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa
    14041 ggtcgtcagg cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag
    14101 gcggcggaag ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg
    14161 gtacacgggc ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt
    14221 gagctacatc tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc
    14281 agcctgatga ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc
    14341 gggcgcgtag gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt
    14401 tctaaaacgg cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt
    14461 gcgcaaaaag atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt
    14521 aatgctgaat ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag
    14581 cgcctggcac gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc
    14641 tggatgccgg agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca
    14701 tttcaggtta ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt
    14761 atgaatcaat acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg
    14821 gcacttctac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt
    14881 aattaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    14941 cgccatgtgc gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg
    15001 cgttgcaccg aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt
    15061 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    15121 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    15181 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    15241 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    15301 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    15361 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    15421 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    15481 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    15541 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    15601 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    15661 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    15721 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    15781 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    15841 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    15901 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    15961 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    16021 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    16081 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    16141 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    16201 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    16261 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    16321 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact
    16381 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    16441 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc
    16501 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    16561 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    16621 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    16681 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    16741 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    16801 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    16861 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    16921 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    16981 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    17041 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    17101 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    17161 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    17221 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    17281 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    17341 aacggagtga tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    17401 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    17461 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    17521 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    17581 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    17641 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    17701 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    17761 cctctttaac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    17821 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    17881 gctctgttag tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    17941 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt
    18001 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc
    18061 cagcccgcga ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc
    18121 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    18181 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    18241 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    18301 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    18361 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    18421 gttacgctga ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag
    18481 cgtaacgccg acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa
    18541 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    18601 ggtcgcctgg aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc
    18661 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa
    18721 ctgtcgatga acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata
    18781 aacgcggaat tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    18841 ggtatggtca gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat
    18901 gcggcaattg ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    18961 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    19021 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    19081 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    19141 gccgccgccg cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc
    19201 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    19261 gccaccgcca gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc
    19321 ggtgtgccgg tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac
    19381 ggcaatattc agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg
    19441 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    19501 gcgggcgtcg tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc
    19561 tcgccgggta cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag
    19621 cgcattccct tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg
    19681 cagaccattt actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc
    19741 accagtcaca gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc
    19801 gtcagcagcg ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa
    19861 tgccgtgcct tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag
    19921 ggtgttatca cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg
    19981 actaccgtcg agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa
    20041 ctgcacctcg cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta
    20101 ttgacggtca aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat
    20161 catcggcgta ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg
    20221 gcatttcagt tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg
    20281 cacacgataa gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca
    20341 ccagcgtttg taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca
    20401 gaagccaaaa cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc
    20461 gtagttagcg taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc
    20521 cgagcgggca aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac
    20581 cagttcgttg tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa
    20641 ccactgaaca tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat
    20701 cgcgaccaga cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc
    20761 ata
//
LOCUS       DAJTVE010000072        20593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000072 DAJTVE010000000
VERSION     DAJTVE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO439_002476"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO439_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6316822.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO439_002477"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO439_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6316823.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO439_002478"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO439_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6316824.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO439_002479"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO439_002479"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6316825.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO439_002480"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO439_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6316826.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO439_002481"
     CDS             complement(8214..9107)
                     /locus_tag="OO439_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6316827.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO439_002482"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO439_002482"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6316828.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO439_002483"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO439_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6316829.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO439_002484"
     CDS             complement(10238..11470)
                     /locus_tag="OO439_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6316830.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO439_002485"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO439_002485"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6316831.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO439_002486"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO439_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6316832.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OO439_002487"
     CDS             complement(14275..16539)
                     /locus_tag="OO439_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6316833.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO439_002488"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO439_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6316834.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO439_002489"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO439_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6316835.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO439_002490"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO439_002490"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6316836.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20576)
                     /gene="ycaL"
                     /locus_tag="OO439_002491"
     CDS             complement(19830..20576)
                     /gene="ycaL"
                     /locus_tag="OO439_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6316837.1"
                     /translation="MLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAK
                     AIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4922 a         5708 c         5029 g         4934 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tca
//
LOCUS       DAJTVE010000073        20348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000073 DAJTVE010000000
VERSION     DAJTVE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            745..1173
                     /gene="uspC"
                     /locus_tag="OO439_002492"
     CDS             745..1173
                     /gene="uspC"
                     /locus_tag="OO439_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6316838.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1180..2604)
                     /gene="otsA"
                     /locus_tag="OO439_002493"
     CDS             complement(1180..2604)
                     /gene="otsA"
                     /locus_tag="OO439_002493"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6316839.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2579..3379)
                     /gene="otsB"
                     /locus_tag="OO439_002494"
     CDS             complement(2579..3379)
                     /gene="otsB"
                     /locus_tag="OO439_002494"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6316840.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3546..4532)
                     /gene="araH"
                     /locus_tag="OO439_002495"
     CDS             complement(3546..4532)
                     /gene="araH"
                     /locus_tag="OO439_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6316841.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4547..6061)
                     /gene="araG"
                     /locus_tag="OO439_002496"
     CDS             complement(4547..6061)
                     /gene="araG"
                     /locus_tag="OO439_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6316842.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6131..7120)
                     /gene="araF"
                     /locus_tag="OO439_002497"
     CDS             complement(6131..7120)
                     /gene="araF"
                     /locus_tag="OO439_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6316843.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7917..8420
                     /locus_tag="OO439_002498"
     CDS             7917..8420
                     /locus_tag="OO439_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6316844.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8499..8750)
                     /locus_tag="OO439_002499"
     CDS             complement(8499..8750)
                     /locus_tag="OO439_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6316845.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8865..8951)
                     /gene="azuC"
                     /locus_tag="OO439_002500"
     CDS             complement(8865..8951)
                     /gene="azuC"
                     /locus_tag="OO439_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6316846.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9214..9537
                     /locus_tag="OO439_002501"
     CDS             9214..9537
                     /locus_tag="OO439_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6316847.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9708..10205
                     /gene="ftnA"
                     /locus_tag="OO439_002502"
     CDS             9708..10205
                     /gene="ftnA"
                     /locus_tag="OO439_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6316848.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10243..10482)
                     /locus_tag="OO439_002503"
     CDS             complement(10243..10482)
                     /locus_tag="OO439_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6316849.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10673..11884
                     /gene="tyrP"
                     /locus_tag="OO439_002504"
     CDS             10673..11884
                     /gene="tyrP"
                     /locus_tag="OO439_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6316850.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11946..12611)
                     /gene="yecA"
                     /locus_tag="OO439_002505"
     CDS             complement(11946..12611)
                     /gene="yecA"
                     /locus_tag="OO439_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6316851.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12807..12893)
                     /locus_tag="OO439_002506"
     tRNA            complement(12807..12893)
                     /locus_tag="OO439_002506"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12857..12859),aa:Leu,seq:taa)
     gene            complement(12906..12979)
                     /locus_tag="OO439_002507"
     tRNA            complement(12906..12979)
                     /locus_tag="OO439_002507"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12945..12947),aa:Cys,seq:gca)
     gene            complement(13034..13109)
                     /locus_tag="OO439_002508"
     tRNA            complement(13034..13109)
                     /locus_tag="OO439_002508"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13074..13076),aa:Gly,seq:gcc)
     gene            complement(13261..13809)
                     /gene="pgsA"
                     /locus_tag="OO439_002509"
     CDS             complement(13261..13809)
                     /gene="pgsA"
                     /locus_tag="OO439_002509"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6316852.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13866..15698)
                     /gene="uvrC"
                     /locus_tag="OO439_002510"
     CDS             complement(13866..15698)
                     /gene="uvrC"
                     /locus_tag="OO439_002510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6316853.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15695..16351)
                     /gene="uvrY"
                     /locus_tag="OO439_002511"
     CDS             complement(15695..16351)
                     /gene="uvrY"
                     /locus_tag="OO439_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6316854.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16647..16823
                     /locus_tag="OO439_002512"
     CDS             16647..16823
                     /locus_tag="OO439_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316855.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16810..17034
                     /locus_tag="OO439_002513"
     CDS             16810..17034
                     /locus_tag="OO439_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6316856.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17101..17823)
                     /gene="sdiA"
                     /locus_tag="OO439_002514"
     CDS             complement(17101..17823)
                     /gene="sdiA"
                     /locus_tag="OO439_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6316857.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18053..18805)
                     /gene="yecC"
                     /locus_tag="OO439_002515"
     CDS             complement(18053..18805)
                     /gene="yecC"
                     /locus_tag="OO439_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6316858.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18802..19470)
                     /gene="tcyL"
                     /locus_tag="OO439_002516"
     CDS             complement(18802..19470)
                     /gene="tcyL"
                     /locus_tag="OO439_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6316859.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19485..>20348)
                     /gene="dcyD"
                     /locus_tag="OO439_002517"
     CDS             complement(19485..>20348)
                     /gene="dcyD"
                     /locus_tag="OO439_002517"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6316860.1"
                     /translation="VTPMAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTA
                     AVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELA
                     TRVEAQGFRPYVIPVGGSNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAG
                     LAVGLEHLMPESELIGVTVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAP
                     GYGVPNDEGMEAVKLLARLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGG
                     APALFAYHPHV"
BASE COUNT         5196 a         5188 c         4682 g         5282 t
ORIGIN      
        1 cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa ttagcattag
       61 aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat gtccttacaa
      121 atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa ctgtactcgt
      181 cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca
      241 cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt tgggtgtatt
      301 tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag gaataatcga
      361 aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc tataaataat
      421 cactttcatg caataccaga taagctattt ttaaacagac acttaccaca caacaaacta
      481 attaaataca aagaaaggca acaatcaact taatgttaat gaaatgtttt acatacatta
      541 accacttgtt taatgcggag aatcagtaca ctattttctt ataattacat ttgaaattat
      601 atgatcaccg ggagtgattg attaattgtc tttactaata atcagactaa tatttacctg
      661 tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa tgtcacctga
      721 agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc tgttgcggtt
      781 acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc agtaaaggga
      841 cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt agccgcgccg
      901 atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct tgataagttc
      961 attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga gttaagcgaa
     1021 catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg taatcacaat
     1081 cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc aagtgaggtc
     1141 gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct ttggaaaggt
     1201 agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct ttaggtcgct
     1261 aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca gcatttctgc
     1321 atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg cagctgcaac
     1381 ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg ccgctcccgc
     1441 aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa catactcttt
     1501 tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag agtagcggaa
     1561 tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg gcgtccagcc
     1621 taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat gacgaatatc
     1681 ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac gaattttacc
     1741 atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct ctggcaaacc
     1801 tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca gttccgcttt
     1861 aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt ctttcggttc
     1921 aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat ggctttttgc
     1981 gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca gacgatcgtt
     2041 ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg tgtcatatgt
     2101 cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga aaccaatgcg
     2161 attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat aatcgtggat
     2221 ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca acgcatttac
     2281 gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc gataatgaaa
     2341 agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt cctgttcgct
     2401 gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg gctgatcctc
     2461 attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca gtgcccccag
     2521 tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg caatccggtt
     2581 agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt ttttgttgta
     2641 atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc agtcgccatg
     2701 atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg ttaacgactg
     2761 cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc ccgataaagg
     2821 gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc ggtttgatct
     2881 cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta atacgttgcg
     2941 ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc gcaaaagcca
     3001 tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc agttgcacgc
     3061 taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca ttgatgtcac
     3121 ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc agtgcgtcaa
     3181 gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg gttgccagta
     3241 gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc ggtttgattt
     3301 ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt tcaggggttt
     3361 cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt ttgcgcagaa
     3421 cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt gcaatgaaga
     3481 acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg ttgtggaaaa
     3541 aagcatcaga caatgcgttt cgctttttgc ttgtaacggt cgaagatcac cgctgccagc
     3601 aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag gttcatggcg
     3661 ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt tccgatgcca
     3721 cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc ataaccaatc
     3781 gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc tatcgcagat
     3841 accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac acccgccaga
     3901 cgcgcagcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt ggttttattc
     3961 agcaacaaac caaagataat cagacacgcg acggtgagcc agattggcgc aggcagaccg
     4021 aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac cgctttaccg
     4081 tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtgt tgtgatcaga
     4141 gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag aatgccaagc
     4201 agcaaccccg ctgccacgcc aatccacagg ctttcagtca ggttgataac caccgccgtg
     4261 gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc ggaagcaagg
     4321 cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc caaccctttc
     4381 atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa gagcaccgca
     4441 aacaccacca gcatgccgta ctgatcccag atacgcccga agctgaatga cgacttaggt
     4501 gcgccagaac ccgatgtaga aacagaagac atcatactct ccttactcag gcaactgcct
     4561 ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc tcgtgtaaca
     4621 attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg ccgaggactt
     4681 caggtaagtc gctggaggca aacagcaccg ccacaccctg cgccgccagc gcataaatta
     4741 cgttatatat ttcgtgctta gcaccaacat caatgctgcg cgtaggttca tccagcaaaa
     4801 tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga tttccgcctg
     4861 agagattcat gatcagttgc tcagcgcccg gcgttttgat gttgagcgaa cgaatgtgct
     4921 gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc acatgtttgc
     4981 gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg ccttccgctt
     5041 tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt ttacgaatat
     5101 cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg ccaaacaagc
     5161 ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca atttcaccac
     5221 tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca tcaagacgca
     5281 ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg tcgcgcccga
     5341 ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag gttttgacat
     5401 aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct tccatacggt
     5461 gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg cggaaaagat
     5521 tgtcgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata attttagcgt
     5581 tacgcgccag cgccttggcg atttcaacca tctgccactg accaatggag agatatttca
     5641 gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc gcctcataat
     5701 tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga tagatgtttt
     5761 ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc acacccgcgt
     5821 taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact acagaacccg
     5881 tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct gcgccatttt
     5941 cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc gtcagcgcct
     6001 taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta gactgttgca
     6061 taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag aggggaattt
     6121 ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta aagttgtcac
     6181 gcgtgatcag caccacgtcg gtaacttcgg taaattttgg cggttcaacg tcttttgcta
     6241 cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt ggcagcaggg
     6301 aaccgtagaa gccggttgcc tgtgctttag acagttcgct caacgcatcc acaccgttaa
     6361 tgccaatgcc gatgatatcg gcagctttaa agccctgacc ttccgtcgcg cgtacgccgc
     6421 ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc ggatgttgaa
     6481 ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg gtaggtacct
     6541 gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagaa cccgttgtac
     6601 ggcgacgtgc cgtatccagt tcgttggcgg taatcgccat caccgcgctt tctttgacat
     6661 cccagccacg tttctgcatc tctttataca gttcctgacc ctgacgttcg ccaattttag
     6721 tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg ttaacaaact
     6781 ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgcgacg atggcagagc
     6841 cgagtttggg gtccggagta caaataacga aaccttttgc gccactggca gccaggctgt
     6901 cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc tcaaacccta
     6961 aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc tcttccggtt
     7021 gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac ataacggctg
     7081 ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc agctttagtg
     7141 tcgttttgtg tagggcaaaa acgaatgact ttcgttaaat taatcggaaa acaaagcatt
     7201 accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa attaattctc
     7261 cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa tttagcggga
     7321 aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc agattaatcc
     7381 ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt cttcttttta
     7441 accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa taaccgcaac
     7501 tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta attatcactg tgctcattaa
     7561 ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt ttataaaatc
     7621 atcacttcat catgaattca aattcattga ttaatatcaa caagatacaa aaagcactat
     7681 cattaaaatt cattgcagtt acattgattt catcaatgaa atgtaaaaat atataaactt
     7741 gatgatttaa gcattttctt atacccgttc agacgttatt cttatttcag atcatcgtca
     7801 gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc aaacagtaat
     7861 tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata ttaggtatgg
     7921 caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca
     7981 atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc accgccactt
     8041 tccttcgcgc ccaggcacag agtaatgtga cccaaatgat gcgcatgttt aactttatga
     8101 agagtgtcgg cgctaccccc atcgttaaag ccattgatgt tcccggtgaa aaactgaact
     8161 ctctggaaga actgttccaa aaaacgatgg aagaatacga gcaacgttct agtacgttgg
     8221 cacagttagc cgatgaagcg aaagaactga atgatgattc aaccgtcaat ttcctgcgcg
     8281 atctggaaaa agaacagcag catgatggtc tgttgctgca aaccattctt gatgaagtgc
     8341 gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga ccaacatgtt ctgaatgtcg
     8401 tgtcacacca gctgcattga tcatcatcgg cgctaatgca ttgcgccgat gaaggttttg
     8461 agaaaccgct gcctcatctg tttgaagcag cggttttttt aatgggattc accctgtggg
     8521 gtaaacttga gttcaataag cgcgatggct ttttggattg cccgcatggt gaccgggtct
     8581 gcggcggcgg gatggttagt aaagtcgata ttcttcagct gactggacat tttttcacga
     8641 acttcaacgg gcgcgattac atcgagaaca tccagaattt gtttgataac caactggcaa
     8701 gcaaccacat cagaaaccag ttcctgatcg gcattcagcg gctgggacat cgtaaactcc
     8761 tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcagcgat agtcacatcc
     8821 accgtcatca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg gaacatattg
     8881 cctggcggta cgtctttgaa cgtcttgcaa tagttattga acatactttt caggattttg
     8941 cgcagtttca tcgcggcact ccgaccattt gttatacagg tgttattgtc tttgcgctta
     9001 taatatgaca accatcacaa aaatcaatct ttatgtgata caaatcacat aaatacccct
     9061 ttaatgttat aaaaatgata atcaaaaaac agccccccta tttctgacac ctacagatgg
     9121 caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg taacgcgtaa
     9181 aatagtgctt tctcttactc ttctggctgg accatgagac ttctgattct gactctttca
     9241 ttaataacgc tggcggggtg tacggttact cgccaagccc atgtgagcga agttgacgcg
     9301 gcaacaggta ttgtacggtt ggtttatgac caggcttttt tgcagcatgc ccataccgat
     9361 cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta tacccacgcg
     9421 gtcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt atgtttgaat
     9481 acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt tctctaaata
     9541 ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg ttttattccc
     9601 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
     9661 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
     9721 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
     9781 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
     9841 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
     9901 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
     9961 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
    10021 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
    10081 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
    10141 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
    10201 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
    10261 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
    10321 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
    10381 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
    10441 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
    10501 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
    10561 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
    10621 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
    10681 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
    10741 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
    10801 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
    10861 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
    10921 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
    10981 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
    11041 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
    11101 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
    11161 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
    11221 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
    11281 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
    11341 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
    11401 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
    11461 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
    11521 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
    11581 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
    11641 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
    11701 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
    11761 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
    11821 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
    11881 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
    11941 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
    12001 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
    12061 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
    12121 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
    12181 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
    12241 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
    12301 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
    12361 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
    12421 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
    12481 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
    12541 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
    12601 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
    12661 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
    12721 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
    12781 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
    12841 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
    12901 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
    12961 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
    13021 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    13081 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
    13141 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
    13201 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
    13261 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
    13321 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
    13381 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
    13441 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
    13501 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
    13561 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
    13621 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
    13681 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
    13741 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
    13801 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
    13861 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
    13921 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
    13981 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
    14041 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
    14101 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
    14161 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
    14221 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
    14281 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
    14341 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
    14401 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
    14461 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
    14521 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
    14581 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
    14641 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
    14701 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
    14761 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
    14821 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
    14881 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
    14941 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
    15001 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
    15061 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
    15121 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
    15181 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
    15241 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
    15301 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
    15361 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
    15421 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
    15481 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
    15541 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
    15601 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gcaggttacg
    15661 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
    15721 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
    15781 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
    15841 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
    15901 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
    15961 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
    16021 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
    16081 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
    16141 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
    16201 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
    16261 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
    16321 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
    16381 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
    16441 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
    16501 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
    16561 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
    16621 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
    16681 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
    16741 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
    16801 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
    16861 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
    16921 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
    16981 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
    17041 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
    17101 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
    17161 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
    17221 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
    17281 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
    17341 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
    17401 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
    17461 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
    17521 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
    17581 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
    17641 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
    17701 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
    17761 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
    17821 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
    17881 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
    17941 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
    18001 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
    18061 gtagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
    18121 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
    18181 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
    18241 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
    18301 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
    18361 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
    18421 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
    18481 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
    18541 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
    18601 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
    18661 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
    18721 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
    18781 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
    18841 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
    18901 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
    18961 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
    19021 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
    19081 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
    19141 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
    19201 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
    19261 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
    19321 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
    19381 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
    19441 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
    19501 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
    19561 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    19621 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    19681 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    19741 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    19801 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    19861 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    19921 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    19981 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    20041 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    20101 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    20161 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    20221 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    20281 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    20341 ggggtgac
//
LOCUS       DAJTVE010000074        20250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000074 DAJTVE010000000
VERSION     DAJTVE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1038
                     /gene="ypdA"
                     /locus_tag="OO439_002518"
     CDS             <1..1038
                     /gene="ypdA"
                     /locus_tag="OO439_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6316861.1"
                     /translation="RHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQ
                     NGDDFISPTTRQAMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHA
                     HQITSSLQEMAVGLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAI
                     SSSIRLNPDTARQLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKL
                     TVIYDIDEEVNCCIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDT
                     GHGIDPKVIERVEANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQR
                     TPVASQATLLL"
     gene            1053..1787
                     /gene="ypdB"
                     /locus_tag="OO439_002519"
     CDS             1053..1787
                     /gene="ypdB"
                     /locus_tag="OO439_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6316862.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1800..2657
                     /locus_tag="OO439_002520"
     CDS             1800..2657
                     /locus_tag="OO439_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6316863.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2660..5155)
                     /gene="ptsP"
                     /locus_tag="OO439_002521"
                     /pseudo
     CDS             complement(2660..5155)
                     /gene="ptsP"
                     /locus_tag="OO439_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5180..6217)
                     /gene="ypdE"
                     /locus_tag="OO439_002522"
     CDS             complement(5180..6217)
                     /gene="ypdE"
                     /locus_tag="OO439_002522"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6316864.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6217..7302)
                     /gene="ypdF"
                     /locus_tag="OO439_002523"
                     /pseudo
     CDS             complement(6217..7302)
                     /gene="ypdF"
                     /locus_tag="OO439_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7317..8564)
                     /locus_tag="OO439_002524"
     CDS             complement(7317..8564)
                     /locus_tag="OO439_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6316865.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8586..8912)
                     /locus_tag="OO439_002525"
     CDS             complement(8586..8912)
                     /locus_tag="OO439_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6316866.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9132..10097)
                     /gene="glk"
                     /locus_tag="OO439_002526"
     CDS             complement(9132..10097)
                     /gene="glk"
                     /locus_tag="OO439_002526"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6316867.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10301..11557
                     /locus_tag="OO439_002527"
     CDS             10301..11557
                     /locus_tag="OO439_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6316868.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11672..11998
                     /locus_tag="OO439_002528"
     CDS             11672..11998
                     /locus_tag="OO439_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6316869.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12139..13377)
                     /locus_tag="OO439_002529"
     CDS             complement(12139..13377)
                     /locus_tag="OO439_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6316870.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13713..14915
                     /gene="nupC"
                     /locus_tag="OO439_002530"
     CDS             13713..14915
                     /gene="nupC"
                     /locus_tag="OO439_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6316871.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14965..17153)
                     /locus_tag="OO439_002531"
                     /pseudo
     CDS             complement(14965..17153)
                     /locus_tag="OO439_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17362..17437)
                     /locus_tag="OO439_002532"
     tRNA            complement(17362..17437)
                     /locus_tag="OO439_002532"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17402..17404),aa:Ala,seq:ggc)
     gene            complement(17477..17552)
                     /locus_tag="OO439_002533"
     tRNA            complement(17477..17552)
                     /locus_tag="OO439_002533"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17517..17519),aa:Ala,seq:ggc)
     gene            17773..18132
                     /locus_tag="OO439_002534"
     CDS             17773..18132
                     /locus_tag="OO439_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6316872.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18134..18526
                     /locus_tag="OO439_002535"
     CDS             18134..18526
                     /locus_tag="OO439_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6316873.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="OO439_002536"
     CDS             complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="OO439_002536"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6316874.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         5035 a         5402 c         5020 g         4793 t
ORIGIN      
        1 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
       61 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
      121 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
      181 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
      241 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
      301 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
      361 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
      421 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
      481 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
      541 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
      601 tatcaaatta aagactatat tgccattgag caggcccgct ttggtgacaa gctgacggtt
      661 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
      721 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
      781 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
      841 gatccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
      901 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
      961 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
     1021 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
     1081 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
     1141 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
     1201 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
     1261 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
     1321 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
     1381 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
     1441 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
     1501 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
     1561 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
     1621 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
     1681 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
     1741 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
     1801 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctgg
     1861 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
     1921 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
     1981 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
     2041 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
     2101 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
     2161 gccagatcca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
     2221 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
     2281 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcgcaaccg cgcggcgatt
     2341 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
     2401 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
     2461 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
     2521 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
     2581 gcgactattt ttgtcgcgtt ttccggcgtc agtttggtct gacgccggga gagtacagcg
     2641 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
     2701 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
     2761 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
     2821 ccagttcaac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
     2881 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
     2941 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
     3001 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
     3061 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
     3121 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
     3181 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
     3241 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
     3301 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
     3361 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
     3421 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
     3481 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
     3541 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
     3601 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtta accatcggga
     3661 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
     3721 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
     3781 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
     3841 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
     3901 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
     3961 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
     4021 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
     4081 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
     4141 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
     4201 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
     4261 tatgcgaggt gcgcccggtt ttctccagaa tcatgcccgc aagatttttc aaatcaaggc
     4321 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
     4381 gattgttgcg cggcttcagt tccggctagg tgatatgcag caactgttcg ctgatatcgc
     4441 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
     4501 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
     4561 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
     4621 ttttgctttc gccgtcacgc tcacgcaatt gctggttcag ttgctcggca agcgttgcca
     4681 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
     4741 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
     4801 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
     4861 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
     4921 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
     4981 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcggccttt gcgttctggc
     5041 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
     5101 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
     5161 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
     5221 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
     5281 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccactgtg ggtacgccag
     5341 tatcggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
     5401 acggaacgcc aatttctgcc gacacggttt caatccacgc ggtaagtttt ggcggcgcaa
     5461 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
     5521 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
     5581 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
     5641 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
     5701 gatagcaacc gaggcggtca tcaaaggctt tccccatcac ccgctggtga gggagaacct
     5761 gaaaagctgt atcaaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
     5821 catacgagcg cgcaccaatg tccacgcgca tggcgctgac gtcattcccc tgccggtcgc
     5881 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
     5941 ggcgggcagc catgcgcacg ttgccaaccg gcagcacatc aattgcccct tcgcgggaga
     6001 tgctgcgcac cataaatccc acttcgtcca tatgcgcaca gatcatcacc ttcggacctg
     6061 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgtact tctttttgca
     6121 ggcgatccgc ttcttccagt aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
     6181 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
     6241 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
     6301 gatgcgcacg cccccttgcc ctggcaaata aatccccggc tccacggtca gtaacatgcc
     6361 tggctgtagc gtcgtggtat cccgcggtga aaagcgtgga tcttcgtgta cttcaatacc
     6421 gatagcatga ccggtgttgt gaccaaaata gtcgccaaat cctgcctcgg taatcacccg
     6481 gcgcgcggcg tcgtcaacct gttagcagcg tacgccaggg cgaatcgcgg agattgccgc
     6541 cagctgtgct tgcaggacaa tctgatagac gttaaacagc gggtgagatt cggcaattac
     6601 cccttcgcca ttcaccagca aggtgcgcgt catatcagag cagtacccct gatagagcgc
     6661 accaaaatca agagtgacaa actcgcctgc tgcaacaatc ttgtcgctgg ctttgccgtg
     6721 cggcagcgcc ccacgccagc cgctggcgac aatggtgtca aaagaggctt tttctgcgcc
     6781 ctgctggcgc ataaaccact ccagttcagc ggctatctcg cgctcgctca tccccgcctg
     6841 aataaagcgg cgaatatgct ccgctccgcg atcggcaatc ccacaggcga ggcggattat
     6901 ctccacctcc tctggcgttt tgatttgccg cagcacatcc ggcgtggcgc taaccagttt
     6961 cgcattgagt tcagacttcc agcggtgcgc ggtttcccaa ctcacctgct ggccttcaaa
     7021 accgagcgtt tgcaactgct catcggcaat gatttgattg acgatagtgg taagcgtgtt
     7081 cgtcgcgtca agcaaatgca gctggtagcc ttgcgcacgg gcttccacct caacgaaata
     7141 gcgcgaatct accagaatgt gcgcactttc acggctaata accacatagc ctgatccggt
     7201 ggagatcccc agatgcggct gtttgttctg ccgtgaggag agaagcactg catccagttg
     7261 ttgcgcctta agccagtcgc gcagcgaagc gagtaatgtc atctggattt tccttcttac
     7321 aggctatcga ttaacaattt acctttacgg aacatcatca gacgcaggaa caccaccatc
     7381 agcgcagtta tgactgcccc cagcgcgatc cccgccatat agacgcccag gttagtgacc
     7441 agcggccatg cccagatagc ggattccggg aaccattgca ctgcgcccag ccagacagcg
     7501 gcggttgagc cgacaatcgc gccgaccata tacgacggaa tggcggtgat ggggctttcc
     7561 agcgcaaacg gaatcgcacc ctcactaatc cccataaagg caaggaacat cgcggtctta
     7621 ccctgcggat aaagttgagc gttgaacagg cgtttgccgg ttaaacgacg gtcgataatg
     7681 gtcgccagac ccagacctat cggcggaata acgatagcga tagaacgtgc agtgaccggt
     7741 aatacgtggt cagtggtaaa gctgaaggca acaaaacctg cggctttgtt gatcgggcca
     7801 ccaaggtcga ttgcggtcgc cgcggcgatc cccattgcgt acatcagcgc gcccttctca
     7861 cctgcggcag tcagcacggt acggataccg ccgttgatcc agccgccaaa gggggtaatg
     7921 acgtagtaca tcgccagcat gacaaaaatg gcggaaagaa tcggtagcaa aaatgtggtt
     7981 ttgaacgcta acaggaaatc cggtaactgg attttctggt tcatccactt cacaaggtaa
     8041 cccgcgacga tagaaataat cagcgcacca atgaaggtgg acggcaccgg cgaagaggtc
     8101 gcccattgca tagtgctggg atcgaagttc agcagttgcg tcggctgggt agacatcaag
     8161 ccgccgataa aaccagccgg gaacgccagt ttgccaccga tagagttcgc cacaaaagca
     8221 gcaaacatcg gaatggcaaa accaaacaac acgccgccga aggactgcga cagccaggca
     8281 aatttcagta aggagaggtc gaaaccggag aatttcccgc tattaagagc atccatgatg
     8341 ccgatctcag caggaatttt aagccagcta taagcaatca gctggctgaa agcgaggatc
     8401 acaccgccca taattaaggt cggcaccatg cgtgaaatcc ctgacatcac atgctgcggg
     8461 agttcacccc agaagctgct tttagaggcc tgcggattct taactgccgc tgccgcaccg
     8521 gatgcgccag gcacaacggt tgcactgcgt tttttaatgg ccataacaat tatccctgtc
     8581 gaatattact gttgttcaga ggcaatcatc tcttcgattt ctttgatgat gcccgcagcg
     8641 tttttaattg cgtcctgcaa agtgatttca taaacgtcgc gtgattcgaa acgctcatta
     8701 tcttccgggg taactgccac ggagtggatg atgatggttg cttcggcgat atcctgcgcc
     8761 gtcaggcgat tctggatacc gtccgcgccc tgggtttcaa ttttcacttc ataacctgct
     8821 tctaccgccg cttcttccag cgcctgagcg gccataaagg tgtgagccag gcccattggg
     8881 caggcacata aggcaattag tttcttactc atcgttaatt cctcgttcag tgatagttga
     8941 cgagtgaaat tatgaacgtg caaaacaaat cgccgttacc agacaaataa tgcatttact
     9001 ggagaattaa tcccgctgta cggaagtgtg acgcaggtcg gccttgtgag ggggacacaa
     9061 agaaaaattg aattaactgt atgatttaaa agattatcgg gagagttacc tcccgatata
     9121 acagaaagga tttacagaat gtgccctaag gtctggcgta aatgtgcacc ggaaccgaga
     9181 agacccggat tgtcatggac gatgagatac accggaatat catggacata ttctttaaag
     9241 cgccctttat cttcaaatgc ggcacggaaa ccagaggctt tgaagaactc aaggaagcgc
     9301 ggcacgatac cgccagcaat ataaacccca ccaaaggttc ccagcgttaa tgccagattg
     9361 ccgccaaaac ggcccataat gacgcaaaac agcgacaacg cacggcggca atcggtgcag
     9421 ctgtcagcca gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg
     9481 tcggctttaa caattgcgcg atacaaattc accagcccag gaccagaaag cacgcgttcc
     9541 gccgaaacat gaccaatttc cgcacgcaat atttcgagga taatggcctc ttcttcacta
     9601 ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc ttacccaacg cttatcgaca
     9661 tggaccagat gcgcaacccc aagccctgtt ccggcaccgt aaaccgcaat aggtttacct
     9721 tcgaccggtt ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc
     9781 atcgatacag cggtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc
     9841 atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt
     9901 gggcaagcga tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg
     9961 cgaatgaccg cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt
    10021 tcaccactgg caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact
    10081 aatgcatact ttgtcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac
    10141 tgtaaatacc tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat
    10201 ctgggtcaca catttacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc
    10261 tgggcaaact aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag
    10321 aaccatgttg ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct
    10381 gattgtggtg atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg
    10441 aactctgggg atagcccagg attcacctat ctggatcatc ggtgtattaa cgctaacggg
    10501 tattgcggtg gggttggtta tccgtttcag ccagggtcat gccggaccag accccgcctg
    10561 tgaaccgctg atcggcgcac cagttccgcc ctctgcgcta ccaggactta tcgtagcatt
    10621 aattctcggt cttgctggcg gcgtcagcct ggggccggaa catccgatca tgaccgtcaa
    10681 tatcgccctt gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg
    10741 gactatttta gcctctgccg gaaccatcgg cgcgctgttt ggtacacctg ttgcggcggc
    10801 gttgatattt tcgcaaacct taaatggcag tagtgaagtt ccgctatggg atcgtctttt
    10861 tgcgccgtta atggcggcag cagctggtgc acttactacc ggattatttt tccatcccca
    10921 tttttcactg cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc
    10981 aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt
    11041 gcacgcgatg atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat
    11101 tctcggaatt ctgggggtta ttggtggacc agtttcgctg tttaaagggc tggatgagat
    11161 gcagcagatg gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtaat
    11221 taaacttgcc gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc
    11281 ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat gagcacgttc ccgccgttcc
    11341 agcggcaata accgtttcct gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg
    11401 ctggttaagt ctttttatgg cggcagtcgt tgtacccaat accacattgc taccgctgct
    11461 ctgtatcgtc atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa
    11521 tcgtccgaag caacagccac cccacgataa tgtttagcaa aaaatgctct cctttatgat
    11581 ttaagagtta tggcgatgat acggggcttt acctccccgt aatattgcgt taacaggccg
    11641 ctgacggctt cgatcgtgaa ggagaataac aatgttcagg tcactgtttc tggcggccgc
    11701 cctgatggca tttaccccgc ttgcagcaaa tgcaggtgaa atcaccctac tgccatcgat
    11761 caaattacaa attggcgatc gcgatcatta cggtaattac tgggacggtg gtcactggcg
    11821 agatcgtgat tactggcatc gcaattatga atggcgcaaa aaccgctggt ggcgtcatga
    11881 taatggctat catcgtggct gggataagcg taaagcatat gagcgtggct atcgtgaagg
    11941 ctggcgcgat cgtgacgatc atcgcggaaa aggccgcgga catgggcacc gccattaaac
    12001 gcgtcctcca atggagcaca atgcctgatg cgacgctagc gcgtcttatc aggcctacaa
    12061 accggctctt taacgcgtag ggcggataag gcgtttacgc cgcatccgcc aatagtgcca
    12121 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    12181 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    12241 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    12301 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    12361 aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa
    12421 gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
    12481 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    12541 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    12601 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
    12661 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    12721 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    12781 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    12841 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    12901 cccgccaatc actttctcca gtggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    12961 agtcgcgatc cccgtcagca ccgcgccctg caacaacgag acaccaagaa tgagtttaaa
    13021 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    13081 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
    13141 ttttccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
    13201 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
    13261 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    13321 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    13381 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ataaacgaga atgattatca
    13441 aattcattta aatggattgt ggtgatttct ctgatagacc aggattatga ctacgaaaag
    13501 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    13561 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    13621 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    13681 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    13741 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    13801 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    13861 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcagat
    13921 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    13981 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    14041 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    14101 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
    14161 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    14221 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    14281 aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
    14341 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
    14401 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    14461 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
    14521 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
    14581 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    14641 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    14701 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    14761 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    14821 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    14881 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
    14941 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    15001 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    15061 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    15121 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    15181 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
    15241 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    15301 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    15361 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    15421 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    15481 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    15541 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    15601 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    15661 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    15721 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    15781 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    15841 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    15901 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
    15961 acccggcaat tgatataact tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat
    16021 tgatcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    16081 atcaatgcgc aggcagcaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    16141 cggaagttgg gcaggagcgt taacggatcg gtcagcgcct gcaaatgcca gcggcgatta
    16201 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    16261 agcaccgcaa gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    16321 ttgtaattca gaaggcaaag cgttgaaaca gccctggtga gatttaaaaa cggatagcga
    16381 agcttaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    16441 aagtcgtttt cataaggtgt gcacaacaga agtagaagca cgcttaacgc tgccagccag
    16501 cttaaggtaa atgcgcgttt ctctttgccc aacgacggcg cgatatccct gcgccacaat
    16561 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    16621 ataagcacag cggtgaaaag gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg
    16681 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    16741 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    16801 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    16861 aacacgacaa acgtctggct accgagcatg accgcagtct gtaaaaaaga gagatgaaag
    16921 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    16981 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    17041 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    17101 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    17161 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    17221 caaacagggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcac
    17281 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    17341 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    17401 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    17461 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    17521 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    17581 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    17641 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    17701 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    17761 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
    17821 tatagccgcc agaccattaa taaatgggta cgcaaggagg gctggacaac gtcaccaaaa
    17881 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    17941 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    18001 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    18061 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    18121 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    18181 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    18241 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    18301 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
    18361 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
    18421 gatagataat atgacagaca ttgagcaggg aaaagtcgcg caatttttgt cacgtgaagg
    18481 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
    18541 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
    18601 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
    18661 cggtaacatc cagtgctgga gactgccccg ccccggttac ggcgacacgc agcggcatac
    18721 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
    18781 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
    18841 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
    18901 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
    18961 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
    19021 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
    19081 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
    19141 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
    19201 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
    19261 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
    19321 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
    19381 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
    19441 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
    19501 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
    19561 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
    19621 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
    19681 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
    19741 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
    19801 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
    19861 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
    19921 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
    19981 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
    20041 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
    20101 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
    20161 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
    20221 actctcccta taatgcggct ccacacagcg
//
LOCUS       DAJTVE010000075        20231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000075 DAJTVE010000000
VERSION     DAJTVE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            689..1459
                     /locus_tag="OO439_002537"
     CDS             689..1459
                     /locus_tag="OO439_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6316875.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="OO439_002538"
     CDS             1450..1923
                     /locus_tag="OO439_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6316876.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="OO439_002539"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="OO439_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6316877.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="OO439_002540"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="OO439_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6316878.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="OO439_002541"
     CDS             3359..3853
                     /locus_tag="OO439_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6316879.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="OO439_002542"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="OO439_002542"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6316880.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OO439_002543"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OO439_002543"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6316881.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="OO439_002544"
     CDS             7698..8612
                     /locus_tag="OO439_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6316882.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="OO439_002545"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="OO439_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OO439_002546"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OO439_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OO439_002547"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OO439_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6316883.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12719..14008
                     /locus_tag="OO439_002548"
     CDS             12719..14008
                     /locus_tag="OO439_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6316884.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14005..15471
                     /gene="hpaE"
                     /locus_tag="OO439_002549"
     CDS             14005..15471
                     /gene="hpaE"
                     /locus_tag="OO439_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6316885.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15473..16324
                     /gene="hpaD"
                     /locus_tag="OO439_002550"
     CDS             15473..16324
                     /gene="hpaD"
                     /locus_tag="OO439_002550"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6316886.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16334..16714
                     /locus_tag="OO439_002551"
     CDS             16334..16714
                     /locus_tag="OO439_002551"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6316887.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16825..17628
                     /gene="hpaH"
                     /locus_tag="OO439_002552"
     CDS             16825..17628
                     /gene="hpaH"
                     /locus_tag="OO439_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6316888.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17639..18427
                     /gene="hpaI"
                     /locus_tag="OO439_002553"
     CDS             17639..18427
                     /gene="hpaI"
                     /locus_tag="OO439_002553"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6316889.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18602..19978
                     /gene="hpaX"
                     /locus_tag="OO439_002554"
     CDS             18602..19978
                     /gene="hpaX"
                     /locus_tag="OO439_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6316890.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19988..>20231
                     /locus_tag="OO439_002555"
     CDS             19988..>20231
                     /locus_tag="OO439_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6316891.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5169 g         4763 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt
    12601 aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact acgcaatcgg
    12661 gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat
    12721 gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg catggcagga
    12781 agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc
    12841 gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaggtact
    12901 gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac gtgaagaaga
    12961 tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc cggaagagag
    13021 cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac
    13081 cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg ggcgtcctgc
    13141 cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga gcgccctgag
    13201 cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac aggcgcgcgt
    13261 ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc cgctggaaaa
    13321 tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc agccacaccc
    13381 gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt
    13441 taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca ctggcgataa
    13501 ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg agctggtggt
    13561 agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg
    13621 ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact accgccctaa
    13681 cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga
    13741 ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca
    13801 gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagtga
    13861 atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag gcttatctga
    13921 cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg
    13981 aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat
    14041 gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt gctggcggat
    14101 gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc
    14161 ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat
    14221 ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac cggcctgccg
    14281 atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga atttttcgcg
    14341 gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac
    14401 acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt accgtttatg
    14461 accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt actgaaaatg
    14521 tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga agccgatatt
    14581 ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga tgcgctggtt
    14641 cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg gcgcaacatc
    14701 atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc gccggtgctg
    14761 atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac catcttctcg
    14821 atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag catctacccg
    14881 gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat
    14941 ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt ctccggctat
    15001 atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga taaaccgtcc
    15061 gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct ggcagacgtt
    15121 gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg
    15181 tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg cctcgcgtcg
    15241 tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagctggc
    15301 atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg cggcgtaaaa
    15361 gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag
    15421 aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa
    15481 gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg aactgccagg
    15541 gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca gcaagcgttg
    15601 ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg tcaacagtgc
    15661 ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca
    15721 ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc agcttattgc
    15781 cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca gcctgaaact
    15841 ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact tcaaagtggt
    15901 ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc
    15961 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    16021 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    16081 gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc agttcaaaga
    16141 gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16201 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16261 caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgcttcccgc
    16321 gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc gcgaagaagc
    16381 cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg gtatttttcc
    16441 gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg ccgacgggca
    16501 gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca gcctggaaag
    16561 ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg ccgccctgat
    16621 ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga cgctgaattt
    16681 taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg tggcgctgcg
    16741 cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc gcagccgcat
    16801 ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct gatcgcccag
    16861 cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct ggattacccg
    16921 gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct gaaaatcgcc
    16981 gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat gcaggcaagc
    17041 tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt ccacgatggc
    17101 agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct ggctttcgtg
    17161 ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa cgccacggac
    17221 tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga tccggaaacc
    17281 cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc cggggtgatc
    17341 ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc cgccctgatg
    17401 tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa tcatccggca
    17461 aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact ggaagccggg
    17521 caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg cgacaccttc
    17581 cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg agagaacgat
    17641 ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat tatggctggg
    17701 gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact ggttgttgat
    17761 cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac aggcgattgc
    17821 gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc aaatcaaaca
    17881 actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg ctgacgaagc
    17941 ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg tgggcagtgc
    18001 gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag ccaacgatca
    18061 aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac cgcagattct
    18121 ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg ccgatatggg
    18181 ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg cgatcgtgca
    18241 gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac tggcaaaacg
    18301 ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc tgctcgcccg
    18361 cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga agcccggcgt
    18421 gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac cgtaggccgg
    18481 ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc ggataaggcg
    18541 ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta gaggaagaaa
    18601 aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc agcataaagc
    18661 gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga gcgtctttct
    18721 gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg ccggactcac
    18781 gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca ccctgttcta
    18841 tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg tcggtgcgcg
    18901 gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca ccatgtttgc
    18961 cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg aagccggctt
    19021 tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc gcgcccgtgc
    19081 caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga tcgtttccgg
    19141 ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc tgtttttgct
    19201 ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg atgactcacc
    19261 ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga tgatggataa
    19321 cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca tgcaacaacg
    19381 cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg cgtatttctg
    19441 cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc aaagttttaa
    19501 tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga tttgtaccat
    19561 tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa ggcatcacac
    19621 cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa ctgatcacaa
    19681 catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg caatggcgat
    19741 tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg gtattgcggt
    19801 gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg gctggttgaa
    19861 agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc tggtgattgg
    19921 tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga ccctgtaagg
    19981 aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt acgatgaaag
    20041 cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat tttttggccg
    20101 ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca atagcggaca
    20161 gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt ttgtcctgac
    20221 gccgccgtca g
//
LOCUS       DAJTVE010000076        20045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000076 DAJTVE010000000
VERSION     DAJTVE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..236
                     /locus_tag="OO439_002556"
     CDS             <1..236
                     /locus_tag="OO439_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6316892.1"
                     /translation="WERARTASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDK
                     LLRDLENQVCDAVVDRVNDHLPGNIDPWKDYNL"
     gene            248..943
                     /locus_tag="OO439_002557"
     CDS             248..943
                     /locus_tag="OO439_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCR6316893.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            954..1928
                     /locus_tag="OO439_002558"
     CDS             954..1928
                     /locus_tag="OO439_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HCR6316894.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            1932..3269
                     /gene="traO"
                     /locus_tag="OO439_002559"
     CDS             1932..3269
                     /gene="traO"
                     /locus_tag="OO439_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCR6316895.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEVLAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            3266..3979
                     /gene="traP"
                     /locus_tag="OO439_002560"
     CDS             3266..3979
                     /gene="traP"
                     /locus_tag="OO439_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCR6316896.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            3979..4506
                     /gene="traQ"
                     /locus_tag="OO439_002561"
     CDS             3979..4506
                     /gene="traQ"
                     /locus_tag="OO439_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCR6316897.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAAA
                     GAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVFN
                     DRVIDATLASLGIAF"
     gene            4553..4951
                     /locus_tag="OO439_002562"
     CDS             4553..4951
                     /locus_tag="OO439_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCR6316898.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            5008..5259
                     /locus_tag="OO439_002563"
     CDS             5008..5259
                     /locus_tag="OO439_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HCR6316899.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            5174..5992
                     /locus_tag="OO439_002564"
     CDS             5174..5992
                     /locus_tag="OO439_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HCR6316900.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            6287..9331
                     /locus_tag="OO439_002565"
     CDS             6287..9331
                     /locus_tag="OO439_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6316901.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            9331..9951
                     /locus_tag="OO439_002566"
     CDS             9331..9951
                     /locus_tag="OO439_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HCR6316902.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPKSLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            9909..11114
                     /gene="traW"
                     /locus_tag="OO439_002567"
     CDS             9909..11114
                     /gene="traW"
                     /locus_tag="OO439_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HCR6316903.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            11111..11680
                     /gene="traX"
                     /locus_tag="OO439_002568"
     CDS             11111..11680
                     /gene="traX"
                     /locus_tag="OO439_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HCR6316904.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11755..13914
                     /locus_tag="OO439_002569"
     CDS             11755..13914
                     /locus_tag="OO439_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCR6316905.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTNTSSTEYRTAEAAEPKSIIMKVWSGENFADKIATSLSGTGTSSD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAKAF
                     LEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNMRRTPGVREEKLKNVNGDDKDGIKQ"
     gene            14002..14649
                     /gene="excA"
                     /locus_tag="OO439_002570"
     CDS             14002..14649
                     /gene="excA"
                     /locus_tag="OO439_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCR6316906.1"
                     /translation="MSTTKSRYPSWWPYYYLVRNAYFIFGIPCLLFYAFFGTLIVHDS
                     ALSQTMDFIFAYGCWALIILPLLWLYLRTRAKKKKVQQVVQQIKESCHFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            14923..16509
                     /locus_tag="OO439_002571"
     CDS             14923..16509
                     /locus_tag="OO439_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HCR6316907.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(16647..16799)
                     /locus_tag="OO439_002572"
     CDS             complement(16647..16799)
                     /locus_tag="OO439_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6316908.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            17129..17371
                     /locus_tag="OO439_002573"
     CDS             17129..17371
                     /locus_tag="OO439_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316909.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            17459..17650
                     /locus_tag="OO439_002574"
     CDS             17459..17650
                     /locus_tag="OO439_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316910.1"
                     /translation="MKKTKTHTGLLIIKDKTRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            17662..18030
                     /locus_tag="OO439_002575"
     CDS             17662..18030
                     /locus_tag="OO439_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316911.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            18034..18249
                     /locus_tag="OO439_002576"
     CDS             18034..18249
                     /locus_tag="OO439_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316912.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(18447..19070)
                     /locus_tag="OO439_002577"
     CDS             complement(18447..19070)
                     /locus_tag="OO439_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6316913.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            19230..19697
                     /locus_tag="OO439_002578"
     CDS             19230..19697
                     /locus_tag="OO439_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6316914.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            19847..20023
                     /locus_tag="OO439_002579"
     CDS             19847..20023
                     /locus_tag="OO439_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316915.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
BASE COUNT         4956 a         5620 c         5145 g         4324 t
ORIGIN      
        1 cctgggagcg ggcgcgcaca gccagccagg catggtcaca gcgggagaat gacgtcgcct
       61 cctccctgca gtcctgcctg tcccgcatca aggacatcag tatcactctt ccgcagttcc
      121 ccagcctgga caagctcctc cgcgacctgg aaaaccaggt ctgtgatgcc gtcgtggatc
      181 gggtgaatga ccatcttccg gggaatatcg acccctggaa agactacaac ctctgaacag
      241 gagcaccatg agcgaagcac aacccacctc ccctgtctca ccggaagcgg ataacagcgg
      301 agatatttat aaccccgtgc tcagaaccat cgcacagtca gaacaacggg ccgcgctggt
      361 acccggtctg gtcagggcgt tgctgtacag cggcgcagca ctctgcatca gcctggtggc
      421 taatggcata cagtactggc atgccaccgg cgtgacgcgc gaatactttg ccactgacaa
      481 tggccgactg gtccgtctgg caccaaccag ccagccggcc tggagtcaga gcgacgtcat
      541 gaactttggc agcatgacgc tgagcgaagc cttcaatctc gattttgtcc actaccgaaa
      601 acaaatcacg acacaggccc cccgcttttc cgaagccggc tttgccggct atgtgaatgc
      661 ccttcaggcc tcgaacattc tcgacactat taaaaaggag aaatttaacc tcacaaccac
      721 tatcggggca ggtaccctgg tcaaccaggg acagatggag aacggcgtct ggttctggaa
      781 attccagtat ccggtcagga tgcgcctggt cggtcagacc agcacccgcc ccgaacaggc
      841 cttcaccttt gaaatcactg tccggcgcgt tgacccccgc atcaaacccg gcggcatgga
      901 aatcagccag atggtttccc gtaacgccgc agcaggaatc tgagactcat atcatgcaca
      961 tacatcatct tattggcgca ctgcttattg tgtgcggcac tgtcacagct gcagataacc
     1021 cgggctggaa aaatgcacga agcccgcaga ccggcggaaa gaccgcacag gcaaccggga
     1081 acaccccggc aggcggtccc ccgccagccg cacaactccc gggaaacctg ccgcctcccg
     1141 gcgctgctct tgtacaggaa ggcgcatcgc tggattctcc cctgaatgcc agtgaaattc
     1201 gtgaactccg tgggataatg gccgataacg aacgggccat cagtgccccg ctcaccagtg
     1261 tggtgccccg tatcagttcc ctcacggtga atctgtctcc gggggccagc ctgccccttg
     1321 tccgtaccgc tctgcataac gtcagcgtgg tgaccttcac ggacgtcaac ggcagtccgt
     1381 ggccacagtc ggatccgccc tataacgctg cgcccggtca ctttgacgtg cagttcaacg
     1441 gcaatatggt gtccgtcacc cccctgcgcg cctgggctgc cgggaatgtg acggtctatc
     1501 tcaaaggatt gtctgtaccg gtcatcctga atatcaccag tggtgaaacg gacaccacta
     1561 ccctgagtca ggaaatcgac agtcgtctgg atctgcgcat tccccggcgg ggacctgcca
     1621 gcccggtggt gtccataccg tcagaaaaaa ttgccctgca tgacgccact ctgcaggcct
     1681 ttctggacgg tgtgccaccg gatgatcccg ccatccgccg cctgaaattc tccggtaacg
     1741 tcccggacac caccatctgg caacacggcg atgatcttct cgtgcgctca cgggccatgc
     1801 tgcgggatga atttgaacag acgctggcat cggctgatgg tacgcggctg tggaaactgc
     1861 cggtgacccc cttactcacc ttctccgtga atggtcagtc cgttcacgtc accccggaac
     1921 tggagtaaat catgagcgca gaacaagaac aggacacagc cgcaaaatcc ggcaaaaagc
     1981 tgtcagccct gctggttatg gggggcgtga ttatcatcgg gggcggatac ctgctgctca
     2041 cctggctgtc ccccgaaaac gctgcatcgc tctcgtcagt caatatcaac agcacaacgt
     2101 ccggcggcgg acggaacgtg atggagacac agcactaccg ggatttactg cgggctgaca
     2161 atgaacgtgg tgctgcagaa gcgttacgta ataacgagac gtttatcgcc agtctgcccc
     2221 ggggactgga taccgatacc ggaaacgatc agaaaaggga taatcccccc gatgatcagt
     2281 caggcacttc cgggcaaaac gcatcccccc ctcaggaaga tcagactgcc cgggaaaaac
     2341 ggcgtgaggc attacagaaa ctgcttgccc gtatcaatac acagcatcag gatgcccggc
     2401 caccggtagt ggcagcagcc atgtggagca ccaccggcac tcccgggaca gccccacatg
     2461 cgtcaccgtc cggtaccgtt acatcacagc atccccgtct gcaggagacg agtctggcaa
     2521 caccggctac acgtcagggg atccagctga taccggcact gacccgcgta ccggcttaca
     2581 tcgacaccga agtggactct gacaatgcgg tctcccgtgt ggtggcctcc gtacctgccg
     2641 gcccatgggc cggggccgtc ctctacagcc aggacgccag actcgcaggt cagggaatgg
     2701 atatccactt tgaccggatg atgtggcggg gcatgtttct gaaggtgaat gcatatgccc
     2761 tgaatgaacg aacaggaatg tcaaccgtgg ccagcgacgt gaaccaccgc tggtttaagc
     2821 acatcgttct gccgtccgta cttggcgggg tgggcaatgt cggaagtctt tacaaagacg
     2881 ccaacacaca gatccttcag ggaaactacg gcagtattac cggcagggtg ggaatgcctg
     2941 atggagaggt gctggccggc gtcattgcag gtggcatggc cgaacggggc agccagctgc
     3001 tgacccgcca ggcagagagc gaaccttacc gtcaggtgac agtatcacgg ggcgaggtcg
     3061 tctccattct gtttgttgag ccggtcatga gcaacgatat cagtacgccg tcctccggca
     3121 ctgccccttc tgagtccccc tcctccggac gtccgctcac ccaggcggag gcagagcacc
     3181 aggcctctga caggatccgg gcggcggtcc tccgtaaaca gaatgaaatg cagcaacgct
     3241 acggcataca acaggaaagt tatccatgaa aaacgaacat gaagatcccg gcagctttgg
     3301 cgaaacgacg gcaccggcat ccggcaaatc cttcctgaaa cgccagtata tgggatacag
     3361 cctgccggtc tgggccggct gtgccctcat catcctggtc gccatctggt acaagtttct
     3421 gcggacaccc tcagagcccc ctgcagatgc aggctttgct gtcacaccgc ccgctgctga
     3481 gagctgggca gagcccatga ccatgacgga gtccgggatc ggaaacaaca tgcccgcacc
     3541 gccggttacc ccacaggcag acatgcagca acttgcccgt gatctgaaag cagagctgga
     3601 cagacgggat gaggaaatcc ggcgcaccct gaccatgctg aaggacagta tcaccaccct
     3661 cagtaacgcc attcagaagg atgaggcctg ggcacaggaa acccgtcagt tgcttgagga
     3721 gacccgtaag caggtgcata ccctgcagat gcagggagca ctgccagccc cgaccgggaa
     3781 taacagacag cctgcgaaaa ccctgccttc acctctgcag ggcatgaaaa tcatgtcggt
     3841 ggagaaaggc atggcctgga tccgctggca gggcagtgcc tgggcagtac gggaaggcga
     3901 cagtctggga aacatcacca tcacccggat tgacccgtcc agccgggcag tcatcacctc
     3961 tgccggtaca ttacgatgat gagcattgat gccctgacag ccatagagaa ctttgccagc
     4021 ggtattttca gtgcaggcat ggagtttctc tttacctggg gagagctgct ggggattatc
     4081 ggccttatcg gtcatctgat gcgcgcccgg gcagaaggac gtcacagtat gggaccggga
     4141 aaatttattg ccggtatctt catctgcggc atgctggtca gtctgccctc aatgatcaat
     4201 gccggtggta cccagatggg cttcagggca gattctttcg cccccatcgc atacgtgcag
     4261 ccgggctcct ttggtgccgc tgccggtgcc gtcaatgcca ttctctcact ggcaaaactg
     4321 gccggcgtcg gttttgtcat gaacggcata tccatctggc gcaaatcact gctggacggg
     4381 cacaccgccc tgagcgccag tgaatccatc agtaaaggga acgtcaagtt tgtggccggc
     4441 gttctgctgg ttttcaacga ccgggttatc gatgccactc tggcaagcct cggtatcgcc
     4501 ttctgacccc tgtatttttt cagtaaatca atctgttaat aaaggagaca ctatgtcacg
     4561 acttcgtgcc atggcggcgc gtctgcgcgc tgcatctgtt tatgttcagt tatgcctgat
     4621 ggcctgccgt gaccggctgg tacgcctgtt actgctgctt cccgtgatgc tgctgcccca
     4681 caccgcaatg gcaaataccc tcttcgatat gattaaaacc gttggcgatg gtgccagagg
     4741 ctccagcagt tcgtttgtgg ctatcggtca ggttattggc ctggtgctct ttatcggcgg
     4801 actgcttggc ctcaaacaaa tcggtaaaaa acccggtgca gggctggcct gcctgctgtc
     4861 aattctggtc ggcgtctgcc ttgccgtcgt accggaactg atgggcagcg gacagaaaac
     4921 gctcggtctt tctccggttt caatcagtta atccgcaaag gcaccgggaa accggtgccg
     4981 tcttcacaca atgaggagga ccagaccatg tcctgtgagc tacccccttt caccctgctc
     5041 agaacagagc acggcaacgt gattgcccgc gacaaccggg ataatcagga gtttgtgttt
     5101 gcagagtgtt actccaccgc tatagcagca ctgattctgg ctgccgtcac cctgtataca
     5161 gagagaaaag cgtatgaaaa agaccccgcc actcatcgcc ccggtccggg aacgatggct
     5221 tttcccgctc ccgcaaccgg acacggtatt cagcggtgaa gccctgttac caccgccagt
     5281 gctctcaaca gagggacgct gcccctgctg tcgcaggacc gtcacccacc gcttcattct
     5341 ggaagattca tggccattac agcagatggc tgacacctgc cgggatacga tcgtcctgct
     5401 ggaaaaaaac ctcacccggg tgatgcgcct gaaaaaacgt ccggtaccgg aaaacgctga
     5461 cgaaaaaaag aaacacaccc gaaccctgca ggatgcggag cggtccctgg cacaggcccg
     5521 cctgtcggcc agacgactgg ccctgcgtca tgtggaaaag tcgcaaatcg tcacaacgga
     5581 tgcactcagc gaaaatgaaa gtgacctgct gcagccggaa ggcccgccct ttcatctctg
     5641 tgccttctgc cacgcctggc actgtctgaa cggctacgct gccgcacagg gagtgatggt
     5701 ctggctcccc gatttgcatc cggcctcggt ggttgccctc aatgcgcggg cactgcagga
     5761 gattttcagc gataaccgtc agcgggtcag gcagggacgg gcagtcctga atgccctggt
     5821 acagaaccgt ctggccgttg aggagaaatt ccgcacctgg cgtccggcgg attttgcaga
     5881 tgccctgcgc cgctggccgc cggcacagcg taaaacgttg agggagaaaa tggacggggt
     5941 ggcgctcatt ctgctgccgg attcgtttcc tgacaaaaag tacgtcatgt gacacacacc
     6001 ggtgccggtg acgttttgcc ggatatcagt tctccgcaga tgcattccgg cactttatta
     6061 tccggtcgct gactgacgat attccgcctg acggagatga aaaagagatt gcccggttca
     6121 ttcgccgcca gcagtttatt tatccgggtg taatcacaca gaaatacgcc cctgcccctc
     6181 aacatattat ttatcttctc tgctgttttt tcatgccaca tcccctgctg tggcatcaaa
     6241 aaatataaga ccttgatgat tcatccttac cggagtgccc catgccatga atatcaaccg
     6301 cattattttt gccattgaag actgtatcag taccctgtca cggtattccg tttccgcatt
     6361 cttccccgat tactgtgatc tgcacaccgt catcggtctg gaccagcagg accgcgaacg
     6421 ccgcccctgg ctgaaagccc cctacattgc caccacaaaa caggggtatt acctttccgt
     6481 ctttgaaatc agcggtgccc tgaaagaaat ggatgaaaac ccggaccaga cggcccccgg
     6541 gacactggag tcactgattg cagcactggc tgagcgaatg aacacggcct ggaaaaattc
     6601 cggccacaaa atcagttttg ttttcgaacg cgatccggag cgtggaaggg atgaaatcga
     6661 agccatgctc ttaccccagc gaaaatccat cgcacgcacg gggatcctgc ttcaggacgt
     6721 cctggatgaa aaagtcacca cactcacccc ctggcttgtc cgggagcgct gctggctgac
     6781 cgtctggagc tctgaagaac tgctgagccg gaccgatcgc aagaaccatc agacccgtgt
     6841 caggaaactc gctgaacacg ctccgccagc ccgttttgcg caggatccct ggcgctggac
     6901 actttccgcc ctcaaaatac ggcatgacgc cttactggac acgctggagc aggctctgac
     6961 acatgattct gatggcctgc tgattcgcct gatggatatc catgaagtgg gacgggaaat
     7021 ccgccgccaa ctggagcgca acagtacacc ggcagtatgg cagcctcatc tgcccgaaga
     7081 tgcccgtcct gccggctggc gacagggcgg ggatacctct gtattccacg caccgtcgct
     7141 gaatctgcag ttattcacca cacagcctga aacacacggc agcctgattc aggccggtga
     7201 gctctggcac ggtatggtcg ccattaccct gccccctcag aatctgcgga cattcaacca
     7261 gctggtcagg gatgtgcccc gtgcaatccc ctggcgtatc cggatggatc tgatgcccga
     7321 cggcatgaag gcgctggggg tcaagaaaac cctgctgacc tacagcagct ttattcctcc
     7381 tctccggccc atgtatgact cggtaatgat gctgaatgac atcaacaagc atgatccggt
     7441 ctgtatcatg accattgttg ccaccacatg gggaaacagc cgggaagtct gtacccgtaa
     7501 ccaggctctc ctgaaatctg cgctggaagg ctggggggta tgcggcacca ccacaacctt
     7561 cggtgatccg agacgtgcct gggtgaacac ggttctggca gcctcccaca gctccggccc
     7621 gatccccctt tacccgcccc tttcacacgc cctttccctg tttcccctga acagggcagg
     7681 ctctgtctgg cggggacagg gaaatctgat gatgcacacc gaagacggct ctgcatggga
     7741 ggtggcgctg gcgtcatcac aacagaacaa gcacacggag ctgacacccg gcgcacccgg
     7801 tctgggcaaa tcggtactca tcaacgcact gtcggagatc cagatagcct cagcccagaa
     7861 gaatctccct ttcattgcct atatcgacaa gggattcagt gcacagggac tggtacagct
     7921 tatccgtgac agcctgcctg agcagagaaa ggacgaagcc gtggggatca ttctgagtaa
     7981 tgacccggac cacacccgca acctgtttga tgtgatgtac ggggccagaa aacccgtcac
     8041 accggaaaag aacttcatgg tctccgtcct ctgtgcattg tgtgtggata ccggcaccgg
     8101 tcagccctgt aatcccggcg acacccggca aatcatcagc agcctggtgg acctggcctt
     8161 ccgggaatat ggcgagaata accccagact ttaccgcgca ggtacggaac ccctggtgga
     8221 tctcgccctg gaagagagcg gcatcgctga acaacatgat gccggatggt ggaatgccgc
     8281 cacctggttt gaaatcaggg acatgctgca tattgcaggg aatatccccg ccgcccagcg
     8341 ggcacactac caggccatgc ccctgctggc tgaaatgtcc gccctgctgg gacagccgtc
     8401 catccgggat gtgttcggca ccgttcagcg tgataactcg gaagagcggt tgctggacta
     8461 catccgccgt gccctcgatc agggacacag tgactacccg atgatgtccg gctgtacccg
     8521 gtttatgctc agccccgaca cccgcgtggt cgccgttgat ctgaacaatg tggccgggga
     8581 caagacacct gccggacgtc tgcgcaccgg tatcatgtat ctgctggccg ggcagatcgc
     8641 cggcggggat tttgtcctgc cgcagtatca ggaggaaata cggaaaaatc tcgacccacg
     8701 ctaccatgag gtgattttcc gccgcattga acagcttgat caggaggtca agaccaaagt
     8761 ctatgacgaa ctgcacaatg caaaagggat caacttcatc tgggaagccc tggacaccca
     8821 ggagctggag cagcgtaagt tcgggatacg cacggtgctg agcacccagc tgctgcagca
     8881 ttatcctccg gccgtgctca aatccgccaa caccctgtgg ctgctgcgtt accgccctga
     8941 cgaaattccg ttcctgcggg ataactttgg cgtcccggaa gtcacactgc ggcgcttcct
     9001 gaaaatgccg gaaggggccg caccggacgg cagcggtgtc ccggtactgg ctgtgttccg
     9061 tgtaaaaaac ggcacgctgg cccggatcct gaaattcacc ctcggtccac tggagctgtg
     9121 ggcactgaac tcctctccta aagacagcgc cctgcgacgt gcgctgacgc aggaggtggg
     9181 gtcactgcgg gcacgccaga tcctggccga acactttccc cggggttcag ccacctcact
     9241 gatagaacac cgcgcccgca cacatgacag tgaaaacgtc atccatgagc tggcagcaga
     9301 actcatccgt aagcagggat ataacctctg atgtacagca ttcatatcac cccccgctgc
     9361 cggcgtcctg cccggggaca ggtcttcctg aaaacactgc gcgtggacgg tgacagtttc
     9421 agcttcaccc tgccgtccgc ctgcttcaca ctgcgaccgt cccccaatcc ccgggtcatt
     9481 tttgccagtg acagacgggc tgacggggag atgagtggta ttcgcctggc cctgcccgga
     9541 gagacaggca cgctgataac aacggccacc tggcaggtta acgtgccgga gcgaccgcct
     9601 tttgatatac agcatgtcat tcactggctt ctgcccgcac cactcactgc gctgatgtct
     9661 gacaacacct ggctgtggcc ggatgtcccc ggcacaccgg cactcacagc cggagactgg
     9721 ccggtcatgg aaagcgacac cccgacactg aaacgcgtga agcgtcgtac cttcacgctc
     9781 atcgataacc gtaaccgtca gggatggatc acgcgccgct tccagtgttt cccgctggct
     9841 ccgctgccga aatcactcag tcatcgcgta ccagacatcc tgtatccccc gaaaacggag
     9901 aaaaaagaat gcaaagacaa tctgcaacca cactgcttgc actccttgta ggctgtgcgg
     9961 ttatccctgc cacccatgcc tactctgtga atgttgtgtc cagtcagccg gtcacccgac
    10021 agatcatgcc ggagctggcc accatacagg gcatcatgga ggaaatggtc cgtatgcaga
    10081 cggccaccgg cacagccatc acccagaact cagaaaaact ggccaccgtc attgcccagg
    10141 acgggcaggc cacccgtcag cagatgatat tcagtaatga aacacaccgc ctcgaggagg
    10201 ccaggaaaag cttcagcgtg ccggactcca tctgcagtga atccgcgtcc ggcattgcag
    10261 cagaaagccg ccgggcagca gcgtctgcag cggcacgtct gagtcagggg ggaggggtca
    10321 gcagtaaacc catccgggaa cgtctctccc gggctgcgga ctccccggtc cgcgaggcgt
    10381 atgatagtgc cggcatccac gccggttact gcacggaagc ggagtatgcc cgctttggcg
    10441 gaactgatgt ctgccctgcc gtgggcgatc tgccgggcgg agacagccag gtacgatcgc
    10501 tctatcaggg cgcaggcaca gcagataccc cggccgctct cacctgggat cagaaacaga
    10561 ttgatgccgc gacggcttat atgaaaaaca ccgccagacc gtcagcgggt cgtgccccgg
    10621 ggaaaggaga agtgggcaca cagaccgggc gcacgtatgt gggcctgcag aatgaataca
    10681 acggcatcat cgatgccgcc tctcatccgc agctgtcact gattgctgac agtaccccta
    10741 atgaagccac ccgtggggca ctgactgaag ccctgcagtc accgtcagct gcggcatatt
    10801 ttgaccggac cgcatcatca gaagcccgca cccgtggcca catgagccag cgggagtttg
    10861 aggcctttga agccggcaga cggtatgcca acaccgactg gcagcaggat ctgcagggca
    10921 tggaaggcga taaccttctc cgtgagctgc tgcgcaccac cgccctgctg aactggcaga
    10981 tgaacgatct gaaagagcag attcgtcagg gtaacgttat cgccgggcag caacttgcgc
    11041 tggccgcacg gcagtattac ggacagcgcc ttggcgaact gtcacaggcc atgagccagg
    11101 ggagcgtaag atgaaagacg acaacagcat cgacgaaaaa aaaccggccc cccgtctccg
    11161 ccgcatcggt gatataggga taaatctgtt cgcaccactg gccgaaaccc gctggatgct
    11221 gcgcagctgc cgccatatgc tggaccagca taaaacgcgc ctgaagcgcc tcatgcccga
    11281 ccggcaaccg gagagtccgg tctgtatgac ctgggaggaa gcggtcaggg ccagcggact
    11341 gacggtcgac gaactggacc ggcgtctgcg acgtcgcagg atgttctggc ggacaggatt
    11401 ctggctgatg atgataccgg tacttctgct gtgtggtatg gcccttgcgg ccaccgcact
    11461 gccggtcatc accctgatac gtatcctggt ctgcctgtta ctgatgagta ccattgcagc
    11521 actgtgtgcc tcaaaagccc tgattgcgtc ataccgcctc tggcagttga atgaacgtcg
    11581 ggtcagcgag gacgaacagg gcacattccc cgattacctg caggcccggc acggctggaa
    11641 acaggccaca tggctggcca ttacccaccg gcagtactga ctgtccggtc tgcaatttct
    11701 tttcctcatt ccgggggctg atgcccgggc atccccttcg gttccggagt taccgtgaaa
    11761 atcataccca gagcactgag tgcaggtctg gcgctggctg ccgtacctgc acaggctttc
    11821 gtcacctatc aggacattgt gagtgccgcc accaatccgg atgacctgtc gcgccaggca
    11881 ctggtcacca tttttggtga tgtggtcaca aatcccctga cagccagcag caacaccatc
    11941 attggcaacc tgttcatggt gtttaacgca attatcgcca cgatggggat agtctggttt
    12001 ctgtttattg gtatccgtca tgctgtccgc acgggacacc agggcagtgt ctttaacaac
    12061 ggacgggacg tcgtcggcat actcagtgtc gtgagtggat ttttgatgat tgtgcccacc
    12121 gcttccggct ggagtctggc gcaactggtc atgctgtggg gcgcatccat catgggcatc
    12181 ggttccgcca atatcatggt tcagacagcg gcagaaaaca ttgctgaagg ctattcaatg
    12241 accgtacagc cagttcatgt ctccacccgc acggcagcac ggggaattct ggagatggaa
    12301 ctgtgcaaac acgccatgaa tacgggcctc aatgacttta accgtaccgc acgttcatcc
    12361 acgccgccca tgagcgaatc ctcacggaca gagaatggca gatataccgt caccgtctca
    12421 aatggcagcg ccacctgcgg gagtgtgagt ctctccgtga aaggaaacgg cagtaacaag
    12481 cagtccgcgc tggcccattt tttcaatccc ttcattgaaa cggaatatca gggggttctg
    12541 acggcacagc gctccgccat ggataccatg ctccgtgacc tggacagcgc ggcgcaggaa
    12601 tttgtgtcgg cataccggac ccggcgcgac acgggtaacg gcaacctgcc ggatattgaa
    12661 agccgtattc agaaggcagc caaccggtat gaagaaacgg tgcagaaggc gttgccggca
    12721 gataacgcag aaactgagcg cagggagcag ctgaaatcct acctgaccac ctacggctgg
    12781 gttgccctgg gtgcctggta ccagaccttt gccaccgcca accagcgact ggaagacctg
    12841 gccaaccgcg cccctaccgt gagtggcgtc tcgtcactgg gcgaagccgg ggatacggaa
    12901 ttcctgaagg aagtcaaggg ggcttaccag gcacagttgc agaattcacc atatactcct
    12961 gcactgggaa caaacacttc atcaactgaa tacagaacag cggaagccgc tgaacctaaa
    13021 agcataataa tgaaagtatg gtcgggtgaa aattttgcag acaaaattgc gacatcctta
    13081 tctggaacag gaacatcctc ggaccaacta aatccactga taaaaatgaa aaatatcggt
    13141 gattacacta tgggtattac tgaaagcata tgggcgggtt ataccaccgt cagggtactt
    13201 tccacagccg gcagcaactc cgtttttggt cgactggtca atgccgtcag cggattatct
    13261 gacgccgcaa aggcttttct ggaagcatcc gcaccaccac tctacttcct gctgtttttg
    13321 ctgttctgtg ccggcttttc gttatccatt tacctgccgt ttatcccgtt tatcttctgg
    13381 atgaccggca tcggcaactg gatagtcagc gtactgattg gatgtgctgc tggccccctg
    13441 tgggccgcca cccatctggg aacaagccag gacaggggaa gccgggctgc ttacggttac
    13501 atctacctga tagacagcat gatcagaccg ctgctgatgg tgttcggatt cttttttgca
    13561 tcggtcgcca tcgtggccgg cggcaccatt ctgaatgcgt tgtttggcgc ggctctggtt
    13621 aatgtgcagg cggactccct tacggggtta ttcagcatcg ctggattttt gctgatttat
    13681 gcccgagtct gtaccacact ggttgcagcc gtttttgcgc ttcaggctta tctgcctgac
    13741 catgtaatta attttctggg cggacgtgac ggtgttaata tgctggggaa tatggtttct
    13801 tcagtcaaag atattattgc cggaggtaat accaatatgc gcagaacacc tggcgttcgt
    13861 gaagaaaaac tgaaaaatgt taatggagat gataaagacg gaataaaaca ataaataaaa
    13921 atatcgaaaa accaataaaa cactattgcc atattcacct gaagagatta aaatatattc
    13981 aggtgaatat gggggtttat tatgagtaca acaaaaagca gatatccttc ctggtggcca
    14041 tattattacc ttgttcgcaa tgcgtatttc atatttggca taccatgctt attattttat
    14101 gcttttttcg gaacactaat tgttcatgat tcagccttat ctcaaacaat ggattttatt
    14161 tttgcatatg gatgctgggc attaataatt ctcccattac tctggctata tctgagaacc
    14221 agagcgaaaa aaaagaaagt ccagcaggtt gttcagcaga taaaagaaag ctgtcacttt
    14281 gctccaacca gcgattatga gcaactgtca ttctcgaaga gttgttattt cgggatcgac
    14341 attaaaaacg gcaccatgct gtatgtgcgc atttatccga acaatgtgat ggatgtgatt
    14401 ggtctggata ttcacaactt tacccgcacg gttgctgaag acggcaagct ggaaatccac
    14461 actacatatg tgagtctgcc gatgatccca ctggaaatct ccggcatctc atcaaggacg
    14521 ctggccaaca ccatgcacac catggctgca cgtggatatg agtacaagga acgtttccca
    14581 cagatgatcc ggtatcgggt taaagagtgg gaaaaagttg ccggtgttcc tgttgcagaa
    14641 gtattttgag gtagctatca actgttagac gataatccgt cagccggttt tctctgaacc
    14701 ggctttttct tttcaggcag aatgcctgtc tgccgtctat ctcaggacgc cgcatggagt
    14761 ctctatgttt tccccacaaa ttcttagcac tgtcttgccc actgtagaca aaatcaatac
    14821 aattctgcat atcagttatc tataaaagat aacaatatca ttataaataa ttatcataac
    14881 cacacactca aggcagtacc tagaaaacat aaagataaaa tcatgtctga atcccgcagc
    14941 aattttccag cacgacatgg tactccatgg aatactgaag agttacagcg tcttctcgta
    15001 tccatagcgg agggcgtctc gctaaaaaaa cttactcaaa cacatcaacg atcgtccggg
    15061 ggaatacgtg gagcaatcaa acgcctgctt ccttctgagt ttcttgagtt caacgatata
    15121 aacacggtga atgatctcag caattacttc aacaagattc ctcgccaaga acacaatagt
    15181 atcatcaata atttaatatc attgagcact gcaaaaacaa ttgctcatga taaagttgaa
    15241 atacaaaaga atagttttaa acaaaatacc ccactccccc tctgccaacc caatacagat
    15301 tttaaagatt atatagccga tgaacatgat gtcattatgc taattcattc tgctatcgaa
    15361 agtttatcta ataaacgaca tcaatatatt cttctaatgc gactcggcac agatgattca
    15421 cctcattctc tggcagatat tgccgtcaaa ttaggaattt cccgtgaaag ggtaagacaa
    15481 cttcaggaac gagcccttgg cttactagct gctaaatctc gctatgaagg gacacctgga
    15541 gcttgcctta aagcccttat taactcaata ggtaattcac catatgatat agcagtatgg
    15601 atttatcgta ttgttcatcg ggatttcgtt acatcctctg agttagccgc caaattcatc
    15661 atttttgccg caggcattcc taaagcaaga cgagatgaaa taaaaaattc gctctcatta
    15721 atatcacaat tacaaaagca gaagttacgt gaacgaagag cagaattagc acacgctcac
    15781 gctcaggaaa gagcccgaat caagaaagaa agagttgagt tcatattcag taaatggcta
    15841 gacaatacac attggccaaa atatatcgca cctcctcccc ccgtagaaca gctatgttca
    15901 cagagagcgg ttaatgatac tgacatatct ggattctttt actctcaaaa attaggtcgt
    15961 acagtccagt acgaatcagg gcttgagttt aaaataatga atctattaga atgttgcgat
    16021 caggttcttt tctatcaaga acaaccattt tctattccat attgttttaa aaacaaatca
    16081 aaaaaatact atccagattt actcattgcg acagttgatg gtcggtgcct acttatggaa
    16141 gtgaagccaa cagaccgaat gaccttgagt ctcacgagaa taaaaagtaa tgcaggtcgc
    16201 aaatgggcac atactcgagg atggggatgg ttggtcatta gtgataggtt ctcgttacgt
    16261 cagctggaag aacatgccat ctcaacaaac acatggaaac ttatagaggc tgaactgaaa
    16321 acatcaagga ttttagcatg gcgtgaaatg atggagttac gtacacgata cgagattcat
    16381 cgtttagatc ttattgcata tatcattcag tctggtgctg aagtggataa cttttatcga
    16441 attaccccga aaatatctaa taatacatct aaccagagac aaccaaatga gagcgactgt
    16501 cataattgat gacgcacagg acggaaaaaa taatagctgc cggacagcag catccagcag
    16561 tcgtccggta aaaaagacaa acaaggcgca ttgaggtcag ccttcgcaac aatccggcgg
    16621 cagatgtcct gccgccgtgt tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca
    16681 aacaccgtat ttccctgctc gatacgaaat ccgcaaagcg aatccctcac catccagaca
    16741 aaacacagta tcgtcacaca gacgacgatt aacatcatta aaaacgttcg ttgtggcatc
    16801 gcagcccctc cattgccttt cggccgtcat cgggctacta taatgttgtc taggcattac
    16861 agtgggccct gggttgattg ataaaaaaat cgcctggggc tttctgctac atgccgtcca
    16921 caaaacgctt tcggcataca gcctcaagcg cccgccgcta ttctaatcgt tttcctgtcc
    16981 gccacaatcc ttttcatgcc accacgtatt ttgtgtcagg gacagaaata aagccggaaa
    17041 gtgactacgt gtgcacacat ctgttttccg ggctgcaggc atcgtgttat tttctgaaat
    17101 gcaaaataat aaaaaacaag gacacatcat gaaggatgaa atacacagta ccagcattaa
    17161 cccggatgtt gttgagcagt ttctggccct tatgaataca cattgtaaat cctcagaaga
    17221 ggcgctcacc atgctgagta cgctttgtta caacctgatg atgacatgcg gtatccattc
    17281 catggacagc cagatgctca atgggcatgg gatatccatg caacttcagg ggattgatat
    17341 tgccgaaaag ccaggcaaga atattcacta acctgaacag ggcctgaaaa cgctgtcttt
    17401 tcaggcctct gcaggcaaaa actgatgaca acaaaatttc tgcgtttgta ccccaccgat
    17461 gaaaaagaca aaaactcaca ccgggcttct catcattaaa gataaaaccc gacgtgtcag
    17521 cctctacgaa acgccaacgg catggtgtat cagggggcag gagtgctaca gcaaaagcac
    17581 tggacgccgc tgtggcagcc acgacagtct gagcagactg cgcctggaca gcattaagcc
    17641 tgttgaatga gtggtggcac catgaaagat aacaacacaa ccgccacccc agaaacctgg
    17701 aaaatgcgtt ttctggccaa cgggacatcc ggcacatggt tttacactga cgacattgca
    17761 gtcactgacg cgtggcttcg cgcgcacccg gatgatgtgg aggttatccc tctctgccgg
    17821 atacagcccc gccagcattc ccatgaacaa catattgaag aactccgcgc agccctggcc
    17881 cggatgctcc atatgcatca tatgatgctg gaaaccacac attaccggga aaatatcacc
    17941 gatgcccgtt gtatccgtga aatgcatgaa gcgccattac aggcgctcaa tattctgtcc
    18001 agatacccgc ataaaaagga cgaggtgtga cgcatgtggc tactgatttt ctggctttca
    18061 tcgcccttta atgtctcttc cggtgatgcc cgtcagccca cactggcggt gcacagccag
    18121 gcctttcata ccgaacgggc ctgccgcgat gcattgcgcg ccattaaaaa ggagaatgtt
    18181 ggcgaatttt ctctgcgggg cgtttgtgtt tctgacggaa atacgaagga ggaggctgag
    18241 agctcatgac ggatattact gacagacata cacttcgccg tatgtcctgg tcggaactca
    18301 tcacggcagg acaggaagca gaatttcagc atgattacga gcgtgccaga atagtgtggt
    18361 cattcgcctt acatgttgca acaaccacga ttaacaaaaa tctggccata gctcatattc
    18421 gacgctgcga tactttgttg cataaatcaa aggctgtacc cggaaacaac ccgtgtggtt
    18481 ccggtcagcg agtctgagac ctcaacatcc cgggtgatga ttgtcattcc ggactgacgc
    18541 agccgctgca catctgcggc aatacgttga ataccaagcc agaacagacc atccagcgcg
    18601 gtgacaatta acccgctttc cagcgccagt ttcagccgtg ctgtcggggc tttaacctgc
    18661 ccggctatat cagtgaaaca acgcgtcgtc atgccatagc tgtccgcccg ccgtattcgc
    18721 gtcgcaagac tgtaactgaa gctatccggc acgccggata agtcattctg cagactgtat
    18781 acacatcccc gctgccgctc attgaataca acttttttct ggctgagctg ccattcccgc
    18841 cgcacgctgt caatcatctg cggtgtccgg gttaacagca gacgaaacgg cagttcaaaa
    18901 ctggtcacat aatcaacatt aagtaaggca atccgcagcc gctccctggc tcctgctccg
    18961 gtatcacggc attcacgcat gacatcttcc cactggcgca tcagtcgcgg tgactcattc
    19021 ctggccctga actgatattt ctcaatctga taatcaatat gttcctgcat accgtcaccg
    19081 gatatctgtt gccgtatcat cacggatatc agcgtactgg aattcatgcc ctgcgtgaag
    19141 tatcacactg ccaaaaaaaa cacacctgaa cgttattatt tcgcaatatt aattgcacct
    19201 gttttcataa ataaaaagga gacgatgaaa tgataaaata tatattttca ctcaccatta
    19261 ttttttccgc ctctctctgt gccgcacaga ttcaggggca cgtcatccgc gttcttgatg
    19321 gtgatacgct ggaaatatta caggacaaaa agcctgtcag ggtccgtctg gctaatattg
    19381 atgcaccgga aaagaaacag ccctttggac gctggtcagc cactcagctg aaggacctgg
    19441 tggccggaaa gcccgtaacc gtgcaatacg cgcagacaga caggtatggc cgtattctcg
    19501 gccgggtcta taccgctgac ggcacagaag caaatcgctg gcaggtccgt aacggcgctg
    19561 cctgggtgta cccggactac aataatgact atactctccc gccgctccag cgtgatgccc
    19621 gcgccctgaa gcggggatta tgggcagata aaaatccggt accaccatgg acctggagaa
    19681 aacagaataa gaaataatta tttgctgata tgaagaaaaa aaccatttca aagaaaataa
    19741 atggcaaaaa tacagcatgg aaaaaattgc catttttacc gccgcgttat ttttaatatt
    19801 tgctgtctgc tggtcgtaat aagcagaata acagaaaggt gtaaaaatga acctggttga
    19861 tgcatttgtc actaaagtga tatccggacc gtatgaagaa tacggaaaat ggtgggtgga
    19921 tgtagaatat atttcctggg gagttccggg taaaagccgg ctcatgtttg actcacggga
    19981 acaggcacag gaagtgcagg aaggttttcg atttttaacc tgagcatatt ttgttaaagg
    20041 gcaga
//
LOCUS       DAJTVE010000077        19668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000077 DAJTVE010000000
VERSION     DAJTVE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..348
                     /gene="radC"
                     /locus_tag="OO439_002580"
     CDS             <1..348
                     /gene="radC"
                     /locus_tag="OO439_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6316916.1"
                     /translation="REFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVH
                     PREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIG
                     RGEYVSFAERGWI"
     gene            565..801
                     /gene="rpmB"
                     /locus_tag="OO439_002581"
     CDS             565..801
                     /gene="rpmB"
                     /locus_tag="OO439_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6316917.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            822..989
                     /gene="rpmG"
                     /locus_tag="OO439_002582"
     CDS             822..989
                     /gene="rpmG"
                     /locus_tag="OO439_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6316918.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1087..1896
                     /gene="mutM"
                     /locus_tag="OO439_002583"
     CDS             1087..1896
                     /gene="mutM"
                     /locus_tag="OO439_002583"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6316919.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(1935..2414)
                     /gene="coaD"
                     /locus_tag="OO439_002584"
     CDS             complement(1935..2414)
                     /gene="coaD"
                     /locus_tag="OO439_002584"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6316920.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2422..3699)
                     /gene="waaA"
                     /locus_tag="OO439_002585"
     CDS             complement(2422..3699)
                     /gene="waaA"
                     /locus_tag="OO439_002585"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6316921.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4112..5170
                     /gene="rfaQ"
                     /locus_tag="OO439_002586"
     CDS             4112..5170
                     /gene="rfaQ"
                     /locus_tag="OO439_002586"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6316922.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5167..6291
                     /locus_tag="OO439_002587"
     CDS             5167..6291
                     /locus_tag="OO439_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6316923.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6284..7081
                     /gene="rfaP"
                     /locus_tag="OO439_002588"
     CDS             6284..7081
                     /gene="rfaP"
                     /locus_tag="OO439_002588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6316924.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7097..8113
                     /gene="waaO"
                     /locus_tag="OO439_002589"
                     /gene_synonym="rfaI"
     CDS             7097..8113
                     /gene="waaO"
                     /locus_tag="OO439_002589"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6316925.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8130..9125
                     /locus_tag="OO439_002590"
     CDS             8130..9125
                     /locus_tag="OO439_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6316926.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9135..9827
                     /gene="rfaY"
                     /locus_tag="OO439_002591"
     CDS             9135..9827
                     /gene="rfaY"
                     /locus_tag="OO439_002591"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6316927.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            9853..10878
                     /locus_tag="OO439_002592"
     CDS             9853..10878
                     /locus_tag="OO439_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6316928.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            10963..11946
                     /locus_tag="OO439_002593"
     CDS             10963..11946
                     /locus_tag="OO439_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6316929.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            11992..13245
                     /locus_tag="OO439_002594"
     CDS             11992..13245
                     /locus_tag="OO439_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6316930.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13315..14286)
                     /gene="rfaC"
                     /locus_tag="OO439_002595"
     CDS             complement(13315..14286)
                     /gene="rfaC"
                     /locus_tag="OO439_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6316931.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14290..15336)
                     /gene="rfaF"
                     /locus_tag="OO439_002596"
     CDS             complement(14290..15336)
                     /gene="rfaF"
                     /locus_tag="OO439_002596"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6316932.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15346..16278)
                     /gene="rfaD"
                     /locus_tag="OO439_002597"
     CDS             complement(15346..16278)
                     /gene="rfaD"
                     /locus_tag="OO439_002597"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6316933.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16492..17688
                     /gene="kbl"
                     /locus_tag="OO439_002598"
     CDS             16492..17688
                     /gene="kbl"
                     /locus_tag="OO439_002598"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6316934.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17698..18723
                     /gene="tdh"
                     /locus_tag="OO439_002599"
     CDS             17698..18723
                     /gene="tdh"
                     /locus_tag="OO439_002599"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6316935.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            18962..>19668
                     /gene="waaH"
                     /locus_tag="OO439_002600"
     CDS             18962..>19668
                     /gene="waaH"
                     /locus_tag="OO439_002600"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6316936.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNY"
BASE COUNT         5300 a         4292 c         4645 g         5431 t
ORIGIN      
        1 cgggaatttt tacaaagcca gctcacgggt gaggagcggg agatctttat ggtgatcttt
       61 ctcgactcac aacaccgggt tataacgcat agccgtcttt tttccggcac gctaaaccat
      121 gttgaagtcc atcctcggga aattatccgg gaagcgataa aaataaacgc ctcggcgctg
      181 atccttgcac ataatcaccc ttcgggttgt gctgaaccca gtaaagcgga taaactcatt
      241 actgaacgga taataaagag ttgtcagttc atggatttac gcgtgctcga ccatatcgtg
      301 attgggcgtg gagagtatgt ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc
      361 gatccttcgg gatctttgtc tgttcgggac ttgagcacat cgctgagtca gcgtatacta
      421 cgccaccttt gagaatctcg ggtttggcat ttgggcctgg caatcgagag ttcacataga
      481 actgcgatga ccgggctgta aagcctgacg aggcgccaat accccatacg aagctcgagc
      541 taatttgatt tttggagaat agacatgtcc cgagtctgcc aagttactgg caagcgtccg
      601 gtgaccggta acaaccgttc ccacgcactg aacgcgacta aacgccgttt cctgccgaac
      661 ctgcactctc accgtttctg ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct
      721 gctaaaggta tgcgtgtaat cgataaaaaa ggcatcgata cagttctggc tgaactgcgt
      781 gcccgtggcg aaaagtacta agtacttaga ggaaataaat catggctaaa ggtattcgtg
      841 agaaaatcaa gctggtttct tctgctggta ctggtcactt ctataccact acgaagaaca
      901 aacgtactaa gccggaaaaa ctggaactga aaaaattcga tccagttgtt cgccagcacg
      961 tgatctacaa agaagcgaaa atcaaataat tctcgctttg atgtaacaaa aaacctcgct
     1021 ccggcggggt tttttgttat ctgcttgccc ccatattgat tgcatctgtt cattcctgga
     1081 gatgctatgc ctgaattacc cgaagttgaa accagccgtc gcggcataga accgcatctc
     1141 gttggtgcaa ccattcttca tgcggtggtg cgcaacggac gcttgcgctg gccggtttca
     1201 gaagagatct accgtttaag cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat
     1261 ctgctgctgg agctgcctga gggctggatt atcattcatt tggggatgtc tggcagcctg
     1321 cgcatccttc cagaagaact tccccctgaa aagcatgacc atgtggattt ggtgatgagc
     1381 aacggcaaag tgctgcgcta caccgatccg cgccgctttg gtgcctggct gtggaccaaa
     1441 gagctggaag ggcacaacgt gctggctcat cttggaccgg agccgcttag cgacgatttc
     1501 aatggtgagt atctgcatca gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg
     1561 atggataaca agctggtggt aggggtaggg aatatctatg ccagcgaatc actgtttgcg
     1621 gcggggatcc atccggatcg gctggcgtca tcactgtcgc tggcagagtg tgaattgtta
     1681 gctcgggtga ttaaagcggt gttgctgcgt tcgattgagc agggtggtac aacgctgaaa
     1741 gattttctgc aaagtgatgg taaaccgggc tatttcgcgc aggaattgca ggtttacgga
     1801 cgaaaaggtg agccgtgtcg ggtgtgcggt acgccgattg tggcgactaa acatgcgcag
     1861 cgggcaacgt tttattgtcg gcagtgccag aagtaattca tacgcgccgg atggcatacc
     1921 atccggcata aacgctacgc taacttcgcc accagcgcct gatggacatt ctccggcagg
     1981 aaatgggtga catcgccctg atggcgcgcc acctctttca ccaacgatga agagataaac
     2041 gaccactctt tcgacggcat cagaaacaca ctttccagtt ccggcattaa gtggcgattc
     2101 atatgcgcca gctgcatttc atattcaaaa tccgccaccg cacgcaggcc acgaatcagc
     2161 accgtagcgt gttgattacg ggcgaagttc gccatcaaat cactaaaccc aaccacttcc
     2221 acgttcccca gatgcgcggt tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac
     2281 atcggttttt tactggggct ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg
     2341 gcgcgcgtca cgatatcgat atgaccattg gtaatgggat cgaaagtacc cggataaatc
     2401 gcccgttttt gcataacaac ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg
     2461 aagcagacgc tgtagcgcgc cctggttttg atacagtact tcaacggcat gacggccata
     2521 gaaactacgg taatcggcgt cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc
     2581 atcggtaacg gtaatcagcc cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt
     2641 aaaagtatgc ggccccatca ataccggaat agcgtgtgcg gcagcttcca gaggattatg
     2701 ccctccacgt tcaaccagtg aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa
     2761 catcaactcg cccatcgtat cgccaaccac aacctgcgtg ctggtggagg ggacttcccc
     2821 tgaagagcgt gtgatatagc ttagtccagc ctggcggaca aggttaatcg catccgggaa
     2881 gcgttctgga tgacggggta ccaggatgag caataaattc gggaattgct gtaataatgc
     2941 ctgatgtgcg gcgatcacca cactctcttc accttcgtga gtgctggtgg caatccatac
     3001 cgggcggtgt ggtgcccact ggcggcgcag cgtcacggct ttagcagcca actgcggcgt
     3061 tacagaaata tcgaatttca ggctaccggt aacggttacc tggttatttt ttgcgcccag
     3121 cgccacaaaa cgtgcaccat cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag
     3181 taagcggcgg acgaatttac ccagtttggc ataacctgcg gccgagcggg cagagagtcg
     3241 cgcgttagcg atcaccagcg gaattttacg tttatgtagc gccgcaatca ggttaggcca
     3301 tagttcggtt tccataatca acaccagttt ggggtcgact ttattcagga aacggttgag
     3361 cgcatcgggc aggtcatacg gcagataaac gtgctgaaca tccttcccga aagccgattg
     3421 tacgcgctcc gaaccggttg gcgtcatggt tgtaacggta atcggtaaat caggataacg
     3481 atggcgcagc gcgcgcacca acgggatcgc cgccagagtt tcaccgacgg agacggagtg
     3541 cagcataatg ccgcctggtt ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg
     3601 ttttcgatag gccggagcct tacgtccgcg cacccagagc cgtatccaga tcagcggctg
     3661 aataaggtag agaagggcgg tgtaaagcaa ttcgagcata gtaaatagct gacttatgga
     3721 tgtgctgggg attctatgta tttagctgtg gctttaccat tacttttccc gtttttgact
     3781 taaatagcaa tttacccgcc aattcttgct gaacatctat actttttttg tatttttatg
     3841 cgattcattg atttctgctg tacgtgcagg aaggaatcgc agagcaacag tgaaagaata
     3901 tggttgctaa tgattttatt tgattgattt ctttattgaa aatcaacaat gtgcttaaaa
     3961 agcattcggg tcggattgcc gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg
     4021 tatcagtatt caggtagctg ttgagcctgg ggcggtagcg tgctttttct gcttcacctt
     4081 aatcggataa tctcaacaaa aagagttact tgtggataag ccatttcgaa gaattttgct
     4141 cattaagatg cgttttcatg gggatatgtt attaactact cccgtcatta gttcgctgaa
     4201 aaaaaattac cctgacgcaa aaatcgatgt gctgctttat caggacacca ccccgatcct
     4261 gtctgaaaat ccagagatta acgcgctcta cggcataaaa aataaaaaag caaaagcctc
     4321 agaaaaaatt gccaactttt ttcatctcat caaggtatta cgtgccaata agtatgacct
     4381 tatcgtcaat cttaccgatc aatggatggt tgctatactg gttcgcttat taaatgcccg
     4441 tgtgaaaatt tcccaggatt atcatcatcg gcagtctgct ttttggcgta aaagtttcac
     4501 ccatttagtg ccgttgcagg gtggaaatgt ggtggaaagt aacttatccg tgctgacacc
     4561 attgggactt gattcgttgg tgaagcagac aaccatgagt tacccgcctg caagctggaa
     4621 acgtatgcgt cgcgaacttg atcacgctgg tgttggacaa aattatgtgg ttatccaacc
     4681 tacggcgcgg caaatcttca aatgctggga caacgccaag ttttccgctg tgattgatgc
     4741 cttacatgct cgtggttatg aagtcgttct gacgtccggc ccagataaag acgatctggc
     4801 ctgcgtcaat gaaattgcgc agggatgcca gacgccacca gtaacggcgc tggctggaaa
     4861 ggtgaccttc ccggaacttg gtgcgttaat cgatcatgcg cagctgttta ttggcgttga
     4921 ttccgcaccg gcgcatattg ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc
     4981 gacagaccat attttctggc gtccttggtc aaataacatg attcaattct gggcgggaga
     5041 ttaccgggaa atgccaacgc gcgatcagcg tgaccgaaat gagatgtatc tttcggttat
     5101 tccggcggca gatgtcattg ctgctgtcga taaattactg ccctcctcca cgacaggtac
     5161 gtcgttatga tcgttgcttt ttgtttatat aaatattttc cctttggcgg tttgcagcgc
     5221 gattttatgc gtattgctca gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc
     5281 cagtcgtggg aaggcgaatg ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg
     5341 cataccaatc acgggcgcaa tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa
     5401 catcccgtcg ataaagtcgt tggattcaac aaaatgccgg ggctggacgt ttattatgcc
     5461 gctgatgttt gttatgccga gaaagtcgcg caggaaaaag gctttttcta tcacctgacg
     5521 tcacgttatc gccattatgc cgcctttgag cgggcaacct tcgaacaggg caagccgaca
     5581 cagctgctga tgctgacaga taagcaaatc gccgatttcc agaaacatta tcagactgaa
     5641 gcggagcgtt ttcatattct gccaccgggg atttatcctg atcgtaaata tagccagcag
     5701 ccagctaata gccgtgaaat cttccgtaag aagaatggaa taaccgaaca acaatattta
     5761 ttgttgcagg tcggttcaga cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt
     5821 gcttcgttac cggattcgct gcgccacaac acattgctat atgttgttgg acaggataag
     5881 ccgcgaaaat ttgaggcgct ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc
     5941 tcggggcgca acgatgtctc ggaattaatg gcggcggcgg atttattact gcatcctgcc
     6001 taccaggaag cagcgggaat tgtgctactg gaagcgatta ctgcaggatt accggtacta
     6061 acaacagccg tttgtggcta tgcgcattat attgtcgacg ccaattgcgg cgaggctatt
     6121 gctgagccat tccgccagga aacattgaat gagattttac gcaaagcgtt aacgcaatct
     6181 tcattgcgcc aggcctgggc ggaaaatgcg cgacattatg ctgatacaca agatttatac
     6241 agtctgccag agaaagcggc ggatatcata acgggtggtc tggatggttg aacttaaaga
     6301 gccgtttgcc acgttatggc gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg
     6361 tgaggttttt cgtgaactgg aaactcgccg cactctgcga tttgaaatgg cgggcaaaag
     6421 ctattttctc aaatggcatc gcggcacgac cctgaaagag ataatcaaaa atttactctc
     6481 attgcggatg ccagtattag gcgcagaccg cgaatggaat gcgattcatc gactgcggga
     6541 tgtcggcgtt gatactatgt atggggtggc atttggcgaa aaaggtatta atccgctgag
     6601 cagaacctcg tttattatta ccgaagatct gaccccaacc atcagtctgg aagattactg
     6661 tgctgactgg gcgaccaacc caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt
     6721 cgcgacgatg gtgcgcgata tgcatgctgt gggcattaac caccgtgact gttatatctg
     6781 tcatttcctg ctgcacttgc ctttttccgg taaggaagag gagttaaaaa tttcggtaat
     6841 tgacctgcac cgggcgcagc ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct
     6901 tattgggctt tatttttctt cgatgaatat cggcctgact cagcgggata tctggcggtt
     6961 tatgaaagtg tattttgtcg ccccgcttaa agacattctc aagcaggaac aaggactgct
     7021 gtcgcaagca gaagcaaaag ccacaaaaat cagggaaaga acgattcgaa aatcgttgta
     7081 atttccggag agaaatatga gtgcccacta ttttaatcca caagagatga tcaataagac
     7141 aatcatcttc gatgaaaggc cagcggcgtc agtggcatca tcattccatg ttgcttatgg
     7201 cattgataaa aactttcttt ttggttgtgg tgtttcaatc acgtcagttt tgttacataa
     7261 caacgacgtg agttttgttt tccacgtttt tattgatgat atccctgaag ccgatatcca
     7321 gcgtttagcc caattggcga aaagctatcg tacctgtatc cagatccatc tggtaaattg
     7381 tgaacggctt aaggcattac cgacgaccaa aaattggtct attgccatgt atttccgttt
     7441 tgtaattgca gattacttta ttgatcaaca agataagatc ctttacctgg atgctgatat
     7501 cgcctgtcag ggaaacttaa agccgctgat aacaatggat cttgccaata acgttgctgc
     7561 tgttgttact gaacgcgatg ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa
     7621 tgaacttgaa aagggttact ttaattcagg tgtcctgtta attaatacac tagcgtgggc
     7681 gcaggagtcc gtttctgcta aagcgatgtc gatgcttgct gataaagccg tcgtttcccg
     7741 tttaacctat atggatcaag atatacttaa tcttatcctg tcagggaaag ttaaattcat
     7801 tgatgctaaa tacaatactc aatttagttt aaattatgaa ttaaaaaaat catttgtttg
     7861 tccaattaat gatgaaaccg tattaattca ttatgtcggc ccgacaaaac cctggcatta
     7921 ctgggccggt tatccaagtg cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa
     7981 aaatgaaccg tttatgcggc caattaattc aaactatgct cgttattgcg ccaagcataa
     8041 ttttaaacaa aatgaaccaa ttaacgggat aatgaattat atttattatt tttatttaaa
     8101 gataataaaa tgatattgag aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt
     8161 atttagcaga taataaaaaa gaaaacgccc cagaactaaa tgtttcctac ggtatcgata
     8221 agaatttttt gtatggtgct ggcgtttcaa tttcttccgt tttgattaat aattcagata
     8281 ttaattttgt ctttcatgtt ttcactgatt atgtggatga tgattattta aagtcattta
     8341 atgaaacagc aaaacaattt aatacctcaa ttattgtata tttaattgac ccaaaatact
     8401 ttgctgatct gccgacgtca cagttttggt cgtacgcgac atacttcagg gtattgtcct
     8461 ttgaatatct gagtgaaagt atttccacac tgctgtatct ggatgccgat gtcgtttgta
     8521 aaggaagtct gaagcctctc acagaaatta tatttaaaga tgagtttgct gcggtcattc
     8581 ctgacaatgt tagtactcag gcggcatgtg caaaacgcct caacattccc gagatgaatg
     8641 gacgttattt caatgcaggc gttatctatg tcaatattaa aaaatggcat gaagcaaatt
     8701 tgacaccgta tttactcacg cttttacgag gggaaactaa atatggctct cttaaatatt
     8761 tagatcagga tgcgttgaat atcgcattta atatgaataa tatctacctc gcgaaggatt
     8821 ttgatactat ttataccctg aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc
     8881 aaaccattac cgataaaaca gtattgattc actatacagg gataactaaa ccatggcata
     8941 gctgggctgg ttatccgtct gcatcatact ttaatatcgc gcgtgaacaa tctccctgga
     9001 agaaatatcc tcttaaagag gcgcggactg ttgcagaaat gcagaaacaa tataagcatc
     9061 tgtttgccca tggtgagtat attaaaggca taacttcatt aattaagtac aagcttaaga
     9121 aataaattac tcttatgatt acaagtatac gctatcgcgg cttctcattt tattacaaag
     9181 ataatgataa taaatataaa gaaatctttg atgaaatact ggcctataat tttaaaacag
     9241 taaaagtatt acgtaatatt aatgatacca aagtatcatt gattgatacg aaatattgtc
     9301 gctatgtttt taaagtcttt gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt
     9361 ttgtaaaagg tgactactat cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag
     9421 gtcttacttt tcctaatgat ttttattttc tggctgagcg taaaattttc aactatgcca
     9481 gtgtttttat catgcttatc gagtatgtcg agggcgtaga gctcaatgat atgccgatca
     9541 ttccagaaga tgttaaagca gaaattaagg cttcaatgga aaaattgcat gccttaaata
     9601 tgctatcggg cgacccacac cggggtaact ttatcgttag taaagatggt gtcaggataa
     9661 ttgacctatc cggtaaaagc tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg
     9721 aacgtcacct gggtattgcc aacgaaataa aagattatgg ctactattca gtaatctaca
     9781 gaaccaaact acgcaaattt attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa
     9841 aacggaacca acatggattt attagctgag agtattactg aagtcgctgt ctctggggaa
     9901 attgctaaca ccgatcgtgt gttaaatatc gcttacggta ttgaccgtaa ctttttattt
     9961 ggtgcggcag tatctatgca atcagttgtt atgcataatc cggaccttgc ggttaagttt
    10021 catctcttta ccgactacat tgatgaagat tatctacaac gtgttaatgc ttttaccagc
    10081 aaaaatgcta acgttgaagt aagaatttat aaagtctcca gtgcctttat tgatatcttc
    10141 cccagcctga aacagtggtc ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg
    10201 agtgaaacta ttgaaaatct gttatatatc gatgctgatg tcatctgtaa aggctcatta
    10261 gctggattgc ttgatattaa ttttgatggg gataagtttg cagctgttat taaagatgtg
    10321 ccttttatgc aggaaaaacc agcgaagcgt ctggctatag agggacttcc agggaattat
    10381 ttcaacgccg gtgtagtata tctgcagctt gaagcatggg cgaaaaatga ttttatgaat
    10441 aaagccattg ctatgctggc aagtgacccg cagcacacga agtataaatg ccttgatcag
    10501 gatattttaa atattctgtt ctttggtcat tgtattttta ttagcggcga ttatgattgc
    10561 ttttatggca tcgattatga gttaaaaaat aaaagcgatg aagattataa aaagaccatt
    10621 accgatgata ctaagctgat tcattatgtt ggcgtaacga agccctggaa cgactggacg
    10681 aattatccct gccagaagta ttttaatgag gcttatcagg cttcttgctg gaatgatgtg
    10741 gcgtttattc cagccacgaa tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa
    10801 aatggtgata cgtttaacgc ttttatttac tttattaaat ttaaattaaa taagtataaa
    10861 agaaaactat ttggataata ataaagatca tggatgtctg agttacaata ataattaagg
    10921 atgagtacta tcatatttta ttagttcagg caagagaaca cgatgagcaa tgattaccct
    10981 ttagtatcca taataatacc gacgtataat tcatctgatt acattactga aactctaacg
    11041 aaattagaaa aacaaactta cccaaatttt gaaattgtta ttgttaatga tggttctaaa
    11101 gataacacat caaacgtttt gagagagtat gggttaaccc actctcgatt aattattatc
    11161 aataaagaaa atggcggtgt ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga
    11221 cagtttatat gttttatgga tgatgatgat gagatagatc ctaactatct gctgaagatg
    11281 tataccagac aacatgagac gggaggggat gcgatttatt gtgggcttta tggccattat
    11341 ataaaagatg gtgttactta ctcacctata aatacagagt ttaatgaagg atctttactt
    11401 ttcgactttt tttataaaaa ggttagattc catatagggt gcttgtttat aagaaaacaa
    11461 cttctggaag agaataatct tttttttgat gaagatttac gactaggaga agatctggat
    11521 tttatctatc gactgctaat tacatgcgat atgtatgcgg ttccatatta tatgtataag
    11581 cataactata gagaaaattc cttaatgaac tcatgtagaa ccatcactca ttatcgacat
    11641 gagtcatttg cgcacgaaag aatctactct tctgtgatgc agttatacaa aggtaacagg
    11701 aaagaagaaa ttcatacatt attgagtcaa aatagagctt atcataaaac tcgttattta
    11761 tggaatgttc tacttaatgg tgattttgaa ctattaaatc aattagttga aagcaatgaa
    11821 aaagaattga atgattgtaa tctttctggc aagagagata aaagacgagc aaaaatatta
    11881 gcatcaaaaa attatattct ctggaggatg gtaagactgg taaataggaa aaagaataaa
    11941 cgttagatct tgcattaaat aatccgtcct cgtagcatag gttgaaacta tatgtcattt
    12001 tgttggaatg aaattaattc tggtataaag tctttaattc tcatattatg tattttttct
    12061 ttgatgactt tgtctttatg ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt
    12121 atatctgcat tgtattttct aataacacca aaaaaaacaa taactaacaa tcctactttg
    12181 ttaattttca tctcattatg tcttttggga atcataaata tcatctggta ttcacattat
    12241 aaagtttcag gctctgttta taccaatgca tatcgtggcc caatggaaac tgggaaaatt
    12301 gccttgtgta gtgcttttat tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa
    12361 ataaaatttg ggaaactaat tctgttcgca tccctggcaa cgcagttact tttttttgcg
    12421 catgccatgt ggcaacattt ctatttaaac gttgaccgtg ttgcattatc agcttcccac
    12481 gctacaacag caggctacat catccttttc ccttctttac tggcatcaat tctcatttta
    12541 aaatccgact ttagacataa aacaacatta tatacaatta acttcatgct tagcttatgt
    12601 gctgtcatag taactgaaac gcgtgcagcc atattagtgt ttccattctt tgcgttacta
    12661 ttaatcgtaa tggatagtta tattaataag cgaattaatt ataagttata ttgttttatt
    12721 gcgattgcat tattagcagg tgtattttct tttaaagata cattgcttat gagaatgaat
    12781 aacttaaata acgatttagt taattattcg catgataaca ccagaacttc agtcggtgcc
    12841 cgtctggcaa tgtatgaagt tggcttaaaa acatattctc caataggaca atcactggaa
    12901 aaacgagcgg aaaaaataca tgaactagaa gaaaaagagc ctagattgag tggcgcttta
    12961 ccctatatag attctcattt gcataacgat ctcatagata cgttatcaac gcgtggtatt
    13021 cctggagttg tattaacaat tttagccttt tcagcaatac tcatatatgc cttaagaact
    13081 gctaaagaac cttatatttt aatcttgctt ttttcactac tggtagtagg cctaagtgat
    13141 gtaatactct tttctaaacc ggttccgact gctgtgtttg tcaccataat attgctttgt
    13201 gcttatttta aagcacaatc agaccaatgt ttattagata aataatgaat atgtgaaata
    13261 aaatcagcaa ggaggctgat tttatttcaa aattatctat taaggggttg taatttaaat
    13321 catagcaacg ttttcttcaa tgaatcgctt aacagcattt gctgtcaatt gagacaactc
    13381 attccccgga gccctacaaa ccatctgatt cttcccatat ccaccaatta atcccggatc
    13441 ggttggtcca taaaccgtga tattgggtct atccagtgcc gccgttaaat ggcttaaccc
    13501 cgtatccacc gacactacaa atttagcccc ggccagcacg cgggcaacgc cttccagact
    13561 catcttcggc aatacttcaa cataagcaaa tccttccgcc agtcgtttcg cccgctcttc
    13621 ctcatgcggc gcgccccacg gaagtttaat ccgtattcct gaatcagcca gtaaaccaat
    13681 caattctcgc cagtgttctt ccggccagtg tttatcatca cgggtcgtcg catgaagaaa
    13741 tacggcatat tcgccagcat ctgtaggcag gttcgtcagg aaatgctgtg caatagcata
    13801 atcgccctgg gtttgcggtt tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg
    13861 ttctacggcg tgctgctgtt ttgcaatatg atgcttacga ttgtaaaaca gactggctaa
    13921 aggttcgcga gcggtttgcc agtccatgcc atgctttacg ccatgcgcca gacgcgtcac
    13981 cagcgccgcg ctttttacca gcccctgagc gtcgataact gcgtcatagt tctctgcttg
    14041 tagcgcttca cgaaacgctt tgcgttccgc ttttatgggg gccgagaacc aggctttacg
    14101 ccagcgacgt attgccacag gaataactcg ctcaacggcg gcgtgccagg aaggaatctg
    14161 tgcgaaccct tcttccacca cccagtcaaa cttaatccct gggattgcct gctgggcatc
    14221 agtgagtgcg ggcaacgtat ggagaacatc gcccatcgac gatgttttaa cgatcaaaac
    14281 ccgcatccgt caggcttcct cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg
    14341 gggagtaatg tcgattaagc tctggtgata accctccgca gcgtcacctt tacgcacttt
    14401 gtgatagccg gtaatcaggc ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc
    14461 cgggctactc ggaccataca gggcaaccag cggacgattg agcgccgccg caacgtgcat
    14521 caggccagaa tcgttagtga caatggcttt acaggctgca atcaggataa ccgcttgatc
    14581 aagctgtgtt tcccccgcca ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc
    14641 aagaatctca ttgcccgctt catgatcttt cgccgagcca aacagaacca cctgataacc
    14701 ttcatcaatc agctgctttg ccagttccgc atagtggtag tgtggccagc gttttgccgg
    14761 accaaactcc gcacccgggc aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg
    14821 attacaggta tatgattttt caccttcgct cacctgcaat tgcggccata acaacggttt
    14881 tggtagatct tgtgcggtac gcatcacgcc tttgtcatag gccagcgcga catagcgttc
    14941 caccattagc ggccaggctt ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg
    15001 catctcgccg cgccagccgg tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga
    15061 tttgaaggag ttgggtaaga cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc
    15121 cagtttgcgg cgttcgccga tttccagcgc tccgtgaccg agaggcatag gaatagcttc
    15181 gttaacttcc ggcatccgcg ataataatgg acggcaccat gccggtgcca tcacatcgat
    15241 tatcgcctgg ggatagcgcg cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc
    15301 gccaacccaa gacgggccga tcaccagtat tttcatgcag agctcttatg cgtcgcgatt
    15361 cagccaggcc atgtattccg ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc
    15421 cgccgcgcgc agatttgtca gatctgcctg agtgaacgcc tggtagcggc ctttcagctt
    15481 atccgggaac ggaatgtatt cgatctggcc tttcttgtga taagccagcg tagcatcagc
    15541 tacagcctgg aaggattccg cacgaccagt accgaggttg aagatgccgg aaacgccatt
    15601 ttccaggaac cacagattca catcagccac gtcgcccaca tagacgaagt cgcgtttgaa
    15661 gttctcgcta ccttcaaaca gcttcggtga ttcaccgttg ttaagctggg tgttgagatg
    15721 gaaagcgacg ctcgccatgc tgcctttatg gccttcacgc ggtccataaa cgttgaaata
    15781 gcggaagcca acaatctgcg agttagcttc aggcaggatt tggcgaacat attcatcaaa
    15841 caggaatttt gagtaaccgt agacgttcaa cggtttttcg tactcgcggg attcaataaa
    15901 gtcggaggtg cgtccgccgt aggtggctgc ggaagaggca tacaggaacg ggatttcgcg
    15961 ctccagacag tagtgcagca gctctttgga gtattgatag ttgttatcca tcatatactt
    16021 gccatcccac tcggtggtgg aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc
    16081 gaactcttcg ccagccataa tctggatcag gaagtcttcc ttatccatat agtccgcgat
    16141 atccagatcc accaggttca caaacttggt gccgtctttc aggttgtcca ccaccagaat
    16201 atcggtgatg cctttatcat tcagggcctt aacgatgttg ctgccgataa agcccgcgcc
    16261 gccggtaacg atgatcataa ctgtaacctt cgaattatgg agtcagagac aatctcagac
    16321 acgaatactt ctatcatatc actacacggc gttggcttca gccattcgcc aaaagtgaga
    16381 ttgatggacg ttatttatcc tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac
    16441 ctataagttt gggtaatatg tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga
    16501 gaattttatc agcagttaac caacgatctg gaaaccgcac gggcggaagg gttgtttaaa
    16561 gaagagcgca ttattacgtc tgcgcagcaa gcagatatca ctgtggctga tggaagccac
    16621 gtcattaact tttgtgccaa taactatctc gggctggcga attatccgga tctgattgcg
    16681 gcggcaaagg cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc
    16741 ggcactcagg acagccataa agagcttgaa caaaaactgg cggcattcct ggggatggaa
    16801 gatgcgattc tctactcttc ctgctttgat gctaacggtg gcctgtttga aacgctgctg
    16861 ggcgcggaag acgccattat ctccgacgca ctgaaccacg cgtctattat tgatggtgtg
    16921 cgtctgtgta aagctaaacg ctatcgctat gccaacaacg atatgcagga gctggaagca
    16981 cgtctgaaag aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg
    17041 ttctcgatgg acggcgtgat tgccaacctg aagggcgttt gcgatctcgc agataaatat
    17101 gatgccctgg tgatggtaga cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt
    17161 ggttcccatg aatactgcga tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt
    17221 aaagcgctgg gcggggcttc tggtggttat accgcggcgc gcaaagaagt ggttgagtgg
    17281 ctgcgccagc gttctcgtcc gtacctgttt tccaactcgc tggcaccggc cattgttgcc
    17341 gcatccatca aagtactgga gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg
    17401 gcgaacgcgc gtcagttccg tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc
    17461 gatcacgcca ttattccggt catgcttggt gatgcggtag tggcgcagaa atttgcccgt
    17521 gagctgcaaa aagagggcat ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt
    17581 caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca
    17641 gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg
    17701 aaagcgttat ccaaactgaa agcggaagag ggcatctgga tgaccgacgt tcctgtaccg
    17761 gaactcgggc ataacgatct gctgattaaa atccgtaaaa cagtcatctg cgggactgac
    17821 gttcacatct ataactggga tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg
    17881 ggccatgaat atgtcggtga agtggtaggt attggtcagg aagtgaaggg cttcaagatt
    17941 ggcgatcgcg tttctggcga aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc
    18001 ggtcgtaccc atctgtgccg caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc
    18061 gccgaatatc tggtgattcc ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac
    18121 gacctggctt ccatttttga tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat
    18181 ctggttggcg aggatgtgct ggtctctggt gcaggcccga ttggtattat ggcagcggcg
    18241 gtggcgaaac acgttggtgc acgcaatgtg gtgatcactg atgttaacga atatcgtctc
    18301 gaactggcgc gcaaaatggg tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat
    18361 gatgtgatgg cagaactggg catggccgaa ggctttgata tcggtctgga aatgtccggt
    18421 gcgccgccag cgtttcgtac catgctcgac accatgaacc acggcggtcg cattgcgatg
    18481 ctgggtattc caccgtccga tatgtctatc gactggacca aagtaatctt taaaggcttg
    18541 ttcattaaag gtatttacgg tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg
    18601 attcagtcag gtctggatct ctcgccgatc attacccatc gtttctctat cgatgatttc
    18661 cagaagggct ttgacgctat gcgttcgggc cagtccggga aagtaattct gagctgggat
    18721 taacacgaac aagggctggt attccagccc ttttgcctga ggataatccg ttaaatatgt
    18781 aaaatcctgt cagtgtaata aagatatcgt aattgcgctg atctcttata tagctgctct
    18841 cattatctct ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta
    18901 atagtttctt aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt
    18961 tatgatgaac agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga
    19021 tttccgcact tgtatggaat ctttaattac gcaaacctgg actgctctgg aaatcattat
    19081 tattaacgat ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta
    19141 tccgcacgtt cgtttgttgc atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg
    19201 gattgaagtg gcaacgggca aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc
    19261 caccatgtac gaaacgctga tgaccatggc gttagaggac gacctcgacg tggcgcagtg
    19321 caacgctgac tggtgttttc gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg
    19381 ccttcgctca accggcgtat taaccggccc ggactggctg cggatggggc tttcttcgcg
    19441 ccgttggact cacgttgtct ggatgggggt ttatcgccgc gatgttattg ttaaaaataa
    19501 cattaaattt attgccggat tacatcatca ggatattgtc tggacaacag aatttatgtt
    19561 taatgcgctg cgtgcgcgat ataccgagca atcgttatat aaatattatc tgcataatac
    19621 gtcagtgagt cggttgcata gacaagggaa caaaaacctt aattatca
//
LOCUS       DAJTVE010000078        19476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000078 DAJTVE010000000
VERSION     DAJTVE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            66..278
                     /gene="cspH"
                     /locus_tag="OO439_002601"
     CDS             66..278
                     /gene="cspH"
                     /locus_tag="OO439_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6316937.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            725..1030
                     /locus_tag="OO439_002602"
     CDS             725..1030
                     /locus_tag="OO439_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316938.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1137..1781
                     /gene="gfcB"
                     /locus_tag="OO439_002603"
     CDS             1137..1781
                     /gene="gfcB"
                     /locus_tag="OO439_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6316939.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1778..2524
                     /locus_tag="OO439_002604"
     CDS             1778..2524
                     /locus_tag="OO439_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6316940.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2524..4620
                     /locus_tag="OO439_002605"
     CDS             2524..4620
                     /locus_tag="OO439_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6316941.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4666..5805
                     /locus_tag="OO439_002606"
     CDS             4666..5805
                     /locus_tag="OO439_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6316942.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5793..6239
                     /gene="etp"
                     /locus_tag="OO439_002607"
                     /gene_synonym="yccY"
     CDS             5793..6239
                     /gene="etp"
                     /locus_tag="OO439_002607"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6316943.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6259..8439
                     /gene="etk"
                     /locus_tag="OO439_002608"
     CDS             6259..8439
                     /gene="etk"
                     /locus_tag="OO439_002608"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6316944.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8554..9852)
                     /gene="appA"
                     /locus_tag="OO439_002609"
     CDS             complement(8554..9852)
                     /gene="appA"
                     /locus_tag="OO439_002609"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6316945.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9932..10024)
                     /gene="cbdX"
                     /locus_tag="OO439_002610"
     CDS             complement(9932..10024)
                     /gene="cbdX"
                     /locus_tag="OO439_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6316946.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10037..11173)
                     /gene="appB"
                     /locus_tag="OO439_002611"
     CDS             complement(10037..11173)
                     /gene="appB"
                     /locus_tag="OO439_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6316947.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11185..12729)
                     /gene="appC"
                     /locus_tag="OO439_002612"
     CDS             complement(11185..12729)
                     /gene="appC"
                     /locus_tag="OO439_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6316948.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12863..13051)
                     /locus_tag="OO439_002613"
                     /pseudo
     CDS             complement(12863..13051)
                     /locus_tag="OO439_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
     gene            complement(13180..13917)
                     /locus_tag="OO439_002614"
     CDS             complement(13180..13917)
                     /locus_tag="OO439_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6316949.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(13871..14071)
                     /locus_tag="OO439_002615"
     CDS             complement(13871..14071)
                     /locus_tag="OO439_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6316950.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            14361..14621
                     /locus_tag="OO439_002616"
     CDS             14361..14621
                     /locus_tag="OO439_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316951.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            complement(14789..15043)
                     /locus_tag="OO439_002617"
     CDS             complement(14789..15043)
                     /locus_tag="OO439_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6316952.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(15079..15261)
                     /locus_tag="OO439_002618"
     CDS             complement(15079..15261)
                     /locus_tag="OO439_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6316953.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            complement(15406..17460)
                     /locus_tag="OO439_002619"
     CDS             complement(15406..17460)
                     /locus_tag="OO439_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6316954.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            complement(17546..18118)
                     /locus_tag="OO439_002620"
     CDS             complement(17546..18118)
                     /locus_tag="OO439_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6316955.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGRHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            18234..18557
                     /locus_tag="OO439_002621"
     CDS             18234..18557
                     /locus_tag="OO439_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316956.1"
                     /translation="MPSDGTAVAANRLATPRNINGVPFDGTQDINITSGITQQDADSR
                     YIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDFIAFRPLQKLVGGTWI
                     TVSQL"
     gene            18609..19130
                     /locus_tag="OO439_002622"
     CDS             18609..19130
                     /locus_tag="OO439_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6316957.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(19162..19344)
                     /locus_tag="OO439_002623"
     CDS             complement(19162..19344)
                     /locus_tag="OO439_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6316958.1"
                     /translation="MTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYYR
                     PLQYLLNGAWVTAASI"
BASE COUNT         4936 a         4923 c         4831 g         4786 t
ORIGIN      
        1 atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt cagtaaggaa
       61 tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa agcggcaaag
      121 gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc gcattcactc
      181 cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc gtaaatggcc
      241 tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct tgtttaaagc
      301 tgactggttt tattggaata gtgcaatagt caacatcata cattataata tcagttactt
      361 ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt tataaatgcc
      421 atttaacagc cagttatagt accacttgaa actatattac caacaataat taatagcttt
      481 cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat gctcacaagt
      541 cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc tttcctaccc
      601 gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa ggatgttata
      661 ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga ataaaggaaa
      721 agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga cgccagctgc
      781 gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta ctggcaccac
      841 aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg ctgctggcgc
      901 tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga cgttggcagt
      961 tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca ccacgtccac
     1021 tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc ggttgcgata
     1081 ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga ttaaaggtgc
     1141 gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac agccagcaaa
     1201 gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc gtagcggatc
     1261 agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa gggcaacgaa
     1321 tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc caggataacg
     1381 ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat aataatctgc
     1441 tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata aaagatggca
     1501 gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct gcgaccctga
     1561 gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc aatgtgctgt
     1621 gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg caaaacacat
     1681 tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta ggcgcagggg
     1741 ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac agtcgtattt
     1801 cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa cggtgactat
     1861 ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg ttgtccagtt
     1921 ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc tgaccgactc
     1981 tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc ttgcttcctg
     2041 ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc agcagttact
     2101 caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg tacgagtgga
     2161 tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac agcgtccggt
     2221 gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc aggcaggccg
     2281 tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca agaataacgt
     2341 tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt ggaataaaag
     2401 acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc atgtactgcc
     2461 cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac gtgttcctga
     2521 ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc cagtgcctgt
     2581 catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga ctttggcggc
     2641 acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt cagcgtcaac
     2701 taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt cccatggctg
     2761 gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg ggaagatttc
     2821 agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct gtgggaagaa
     2881 ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac gggtctgttt
     2941 gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac cctcgggatg
     3001 gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg ggtgagcgat
     3061 aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag cgatatcttt
     3121 cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa tcccctgcgt
     3181 ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa actgcctcag
     3241 gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga tctcaacctg
     3301 agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa tttcaacgat
     3361 ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc tgaatctgaa
     3421 ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa cgcaggtttt
     3481 gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca acaatacaaa
     3541 taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa taacctgccg
     3601 caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat ggtgactacc
     3661 gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg tcaatcagag
     3721 caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg gggctaccgt
     3781 attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc gctgggcggc
     3841 ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta ctggtttacc
     3901 gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta cgacaagttt
     3961 aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca tatccgtgat
     4021 tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc cgacttaggc
     4081 aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc cggtgtcggt
     4141 tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga cgttaactac
     4201 gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac gccaactggt
     4261 ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa acttagcgtt
     4321 gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg ctttgacagc
     4381 ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga ctacggcgaa
     4441 ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac cattggacct
     4501 aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg acaaatgctg
     4561 tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt tggacaataa
     4621 cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa aaagaacatt
     4681 tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac ccttgttcct
     4741 ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa tcagtatgac
     4801 ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca gttgcgcgcc
     4861 aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa ctatgaatat
     4921 cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga actaaccacc
     4981 cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc cgatggtgcg
     5041 attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac ccaggtacgc
     5101 aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga tgtcagcgtc
     5161 gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc gggtcagcag
     5221 ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg cggtctgacc
     5281 gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac caaagtgaac
     5341 ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca cccgggcgat
     5401 attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga agtgggtaaa
     5461 cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct gggtaacgct
     5521 gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg tgcaacgcaa
     5581 aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga cgcatcggca
     5641 atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt cactaccgcg
     5701 ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc cggggtgcat
     5761 gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt aactcaatcc
     5821 tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg ctgcgtaaac
     5881 gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa caccctgcag
     5941 atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga catgccggac
     6001 gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg gagtcggaac
     6061 atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg ctgtttgggc
     6121 aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac gcatttgaac
     6181 atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc agccgataat
     6241 ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg cagcactcag
     6301 gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg taagtttatt
     6361 atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt aagcacacca
     6421 atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc cattctcagc
     6481 ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat ccaactgctg
     6541 caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga catagttgag
     6601 cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga aaaaccaggt
     6661 gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca gcaactgaca
     6721 ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac cgtcaatggt
     6781 atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga cattaaggcc
     6841 aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat taacgcattg
     6901 caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga acttaccatg
     6961 actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa caactatttg
     7021 caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt cttacagcgc
     7081 cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa cgtttatcgc
     7141 cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga gcagattgtg
     7201 aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc ccagctgtat
     7261 aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct ggagcaagaa
     7321 cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga agtgttgcgt
     7381 ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa ccgccagcag
     7441 gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga cccggcagtc
     7501 actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg ttttattctt
     7561 ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg tggtgtagaa
     7621 gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc aatgtccgag
     7681 tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca gcagcgccat
     7741 cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc tgtggaagcc
     7801 gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa taacattctg
     7861 atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac tctggcagcg
     7921 gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg ccgtggttat
     7981 tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct ggcaggtaaa
     8041 gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt gattactcgc
     8101 ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg tcaattactg
     8161 gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat gctggcggtg
     8221 agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc gcgttttggc
     8281 ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc aggcgtcaat
     8341 attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta cagttacggc
     8401 tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg gccggatgcg
     8461 gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat cagcctcaga
     8521 gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg gtatgcgtgc
     8581 ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat ttcgctcttc
     8641 acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca gcggcgtttt
     8701 atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact ggctgttatc
     8761 gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt tatccggctg
     8821 accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat tagtatcgtg
     8881 tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt tttgcggtgg
     8941 atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc tgcgggcaac
     9001 ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca aggtgttcca
     9061 ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg cttgttgcag
     9121 gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg agacattgtc
     9181 ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt cgtcctgttt
     9241 ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca gttcgcgaaa
     9301 cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc tgaggatcgc
     9361 gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat taaataacgg
     9421 atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag gtgccagccc
     9481 ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa taatcgcgac
     9541 ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca gacgctggcg
     9601 ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc aacccagttt
     9661 taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg tggccttggt
     9721 tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca gctccggctc
     9781 actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa atgggattaa
     9841 gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt gcctgatgcg
     9901 atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg tccagccata
     9961 ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa agtaaatacc
    10021 acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt tctgttgtca
    10081 tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg ggcaaaaata
    10141 tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg tcccacaacg
    10201 tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc gtgatgcctg
    10261 ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa cccgggcgac
    10321 cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc gggaagatcc
    10381 acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact gccaccagtt
    10441 tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag ccatcaatac
    10501 cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt gcagcgcgtt
    10561 tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt aaccagacgc
    10621 caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg aatggcgtca
    10681 gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag gcaaacggca
    10741 cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc agactgccga
    10801 tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt ccgcgataat
    10861 caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc acataaaagc
    10921 cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca ccagcgagga
    10981 tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact atccggcgtt
    11041 catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca aatccgtcgg
    11101 agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag cgcaatgttt
    11161 cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt cactctgcat
    11221 cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga ttaagaacag
    11281 ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg ttaaagcgga
    11341 gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac caaactcggt
    11401 cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga gcgccatttt
    11461 cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg caatcagcat
    11521 caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga ataccggcgc
    11581 aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga catgattcat
    11641 atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca gttcccggaa
    11701 tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta acagccaggc
    11761 catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca gacccggcac
    11821 ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt tgatggcaaa
    11881 ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg caggagcagg
    11941 ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt gcgcgacttc
    12001 atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac cgaagacgga
    12061 accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta aataccaggc
    12121 gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg tgtgcacaaa
    12181 tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct ccatacgtag
    12241 ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg cattcaatat
    12301 ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca ggtgctggta
    12361 tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt cgaggaagaa
    12421 ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat agttggaata
    12481 gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca cgccaagagc
    12541 aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga ttgttttgcc
    12601 ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca gggtaagggg
    12661 tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata aatcaatgac
    12721 atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat gttagatctc
    12781 cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat tcataagact
    12841 gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac tgacatacct
    12901 cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc acttccggta
    12961 ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta cagcgtaaat
    13021 gccagacatg gcgtaacccc gtggcgttga ttatagcgcg gcgatttttt tgataccgat
    13081 ttgcatttca tttgataccg ctgaaaaaca ccgatacgca acttatttga tactaaatcg
    13141 gcgcttttct tttgagtttt agaatgagtt tttaagcgtt cattcactct ctttcaaacc
    13201 agttttaact gtttgtggtt ttgggtaagg ctggactttg aatagctttg tgttaaggcg
    13261 ttttctggct ctcttgtcca gaaaccggat gtaacgaaac tgacggaatt tatgaacact
    13321 ggctctgtcg atattagccc gcagatgttc acctcgctgt cctcctcgtt tgattgcatt
    13381 cttgcaaatc tcgtgatacc actcgccatc aagttcatag aatgttgttt catgactgcc
    13441 tacataatca aaattgcttg cctggtacac gacaccaaga cagccacaac gctcatctgc
    13501 aaacgactga acccactgca cctgcggata aagttgtctg attagtttga gcgcgtaact
    13561 gattgccctt gattcggagt ttctcggcat acagtcatgc agccataacc ggttaagctc
    13621 catatattcg cggttctgcg tgccagttac gacgcgtgca ccattgtttg gattaagggc
    13681 ataaccccat tgcattacgc caaccagctc ccgttctgaa aatatgccca gatgaaggta
    13741 ggaattattt acgaaacgat ggctgtaatg tttattgacg acgaccagcc gggccagcca
    13801 gcaacttatt gtctcaaccc gtaactcacg ggagccataa ccgacgatat tgtcgttata
    13861 gtgaataagt tcaggcgtgc tgatgatacg ggatgtaact tgctttctgt tccccacgac
    13921 aggatttcct tgtgtatgtg gggtgctctg tggcgctctg agatgtgatt tgattgaggg
    13981 ttttacagcg cggacattta acttccagat aactgaagct ggctcgtgcc agtagcttgt
    14041 tacaatgtcg gcaccgtatg tttcgataca tggccgcata acctcctgct ttgttgttga
    14101 tatctttatc actcaatcga tcgacaaaaa cgatcgattt gatttattta attgatacaa
    14161 caaataacac gaaatgtcat tagcgaaaat ttatgagttt gatcactaat tttaaacggt
    14221 gctgtaacgc ataagtgctg aatgattgta catttgaata ccgcaaagca aacaaatcaa
    14281 ctaatttata gaagtcaatt ggttaattgc cagcgtaaga ggctgctatg gacaatcatg
    14341 taacgacgag aaatcgaaaa atgattgaaa atgcggaaat ctataatgat tatttttccg
    14401 attattcaga tgcctgcaaa tacagttcga gtgcgggaga gttcatatgc ttcaactttt
    14461 ttattaaatc ttatgttttt cctccacagc aagaagatgg aggtgtgcca cctcctgaaa
    14521 tcactttgca ccgtatagcc tccgtctcaa tgatggagca acaggctaat gagctgtata
    14581 gcattctggg aacgattcta caaaagcgtg aatcggacta atgataccgg ttcatttgcc
    14641 tgcccggatg gggaagcagg attgactgct ccataagcag ctatatttct gaatacgaaa
    14701 tactatccag cgattacata tggctggata gcggcaaaga tgttttcatt agtgtcaaac
    14761 cggcagagga caccaccggt ttgtattgtt acatcatggt actgtcagtt ccgggctgag
    14821 ccgggtctgg ttgctctggc tgcgcgtcgg ttttcggctc attatcagca tcactggccg
    14881 caactgctgg cccaataagt tttgccagca cttcatcaac gtaagtgtcg atttgtgcct
    14941 caaaatcctt gcggacctgc gctttcagca acttatttac ttcaccggaa tacagtgcct
    15001 gttttaccag gtcttcagtg acggtgcctt taatttctgg catgtatatc ctccttatgc
    15061 gggaataaag ccctgcaatc agggctgggt cgggtctttt gggagcggag ttacctgcgc
    15121 tgttttactg agtttttcag ctgctgcgtc ctcaattttg cggcgaacat aatttctgat
    15181 ggccttataa ccaccactca ccagatataa cgcacatact gccgtgcaga aatacaataa
    15241 aataagctgt aaaaatgtca ttatccctcc caattattga catggtgttg acaccggtaa
    15301 tatctgttag gtaaaaaggt gcactgcatt ttttgctttg gatataacga tctttttgcc
    15361 gccggtttct tgttttccct taccggcggc atttttttat cctgcttacg cgttattcac
    15421 ttccaccacg atactgtcaa tcagtaccgg gtaagtcgca tttttagtga tatcggtcac
    15481 gcgcaacttg tctgccgtaa acgtgccgac cggagactgc gacagcatga atggtgtccc
    15541 gtccttacca tcaatgaccg gcgtcacctc aatactgttg ttaccggcaa aacggaagcc
    15601 cagcgtatgc cattcgttat taaatgcgcc gaatgacccc agcttcgtgt tctgcgccgt
    15661 gtttcctttg tgatacatca cgttaaggtc tgtggcatcg ctctgtacgt agaacgatgc
    15721 cagcaggtta tgaccggcat ttccttccag agtaacccct tgcggcaggg cagaaaccgg
    15781 ccagtacagc gccagtgcgt actgattagc cgtaagcgcg ccatcgagtt taaaacgaca
    15841 actgacaaga ccacctttac ccagcaggtc cgcaccatta ccagcatcat gctcaaggta
    15901 ccagggggct cttcctgttt ccttggtcag tttcattgcc ttacccccgc tggctccggc
    15961 atcatccacg atttgagcct tgccacctcc ggcagcccaa ccctgtggtg tcaaccgtcc
    16021 ttcagactca gatgcccggt aagcaaatag cgtactcatc gtcgtggtgt ctgcgggtgg
    16081 cgtggacggt tttgatggtg tatcaggtga cggcttctca tccggtggtg tcacggtctg
    16141 cccgcgcatc agttcagccg tgcgccctgc atggagcata atagctgagg ccagacggtc
    16201 tgaaatgacg cccctgcgtg cccatgagct gaaatggctg tcacgctgtg tcgaaacaaa
    16261 actgccctgg gttcgggatg ctgcaccgta atacccgaca gccacaatat ccgggtcttc
    16321 cgccggagca ttcgttggcg tgctctgtcc attctcatcc gtcaggaacg gtacaaagaa
    16381 aattttctgt gcttccttgc ctttgtagcc accgtatact gcctcatatt tatcggtgcc
    16441 agcgtttttc cagtaatacg tggtatcacc gcaaatccac ggcaccgtgg tggcatttcc
    16501 actcacgcac tgcgcagcaa gcggagccag gtcagttctg aactgctgaa ccatggccgt
    16561 gaataaacta ttatgctgct ggcttcctga ggcaaggtct gcctctccct gcatccagac
    16621 cacagccaga agtttgtttt tcgggttttt cgccagcgcg actttggtgc ggcttaccag
    16681 gtcctgatac agaggtttac cggcccccca gcgtgaagaa tcagcggaag caccgctggc
    16741 ctcactgaat gagccgtcgg ctccagtggt gaatgctgac ccgccacgac aacagggcac
    16801 cagaagtatc ccggcattct gtgggatata gggaagcagt tttttggcaa tatgcaaccc
    16861 ctgaccgaca gtcccgtact ggccttttgc cagatcagct ttcgggtggt ttatcccgct
    16921 catatcctga acatcatgca ggcagtggtc tgccggaatg atgtcgttgt atgtacacga
    16981 cgtaccgttg ggcgttaccg tactgcggcg tgccagttgc ttaatgcgtg ggtccgggcg
    17041 gtcaaacgaa tccggtaacg gcagcccctc gccgtaggcc atgccatttg actgacccgc
    17101 gagaatgaca acaaagtaat attccggctc tgaactgctg ccattcgtgc taccggaagg
    17161 cccggcgtta tccggtgcat tccagtcgga gggcgtgagc gttccggcca cgtcagatgt
    17221 gattggaatg atgccagtca cgcctgaact ggtgatttct gcactgggat taccggatac
    17281 cggcattacc cataatggct ctttcgtgct gaagcggatc acgcagtctg cgaaggtgat
    17341 ccccccttta ttacccgaag ggcggaaagg gctttcaata aatgccaccg tgccaccggc
    17401 aaccttgact gaaaactctc cgggcatggc ggagagcatt ttccacttgg agtctgacat
    17461 cgtcttttct gcctgtttct gaattaaaca gagtcagaat agcgaccgct gaaaaatatg
    17521 tgttttgcgg aaattcaaaa aagcgtcacg gtacttttgg tgacggagct ggtgaggcag
    17581 gaaaataacg ataaagggtg gaaaggccaa tatcaaatat cagtgcgatt tgtttacggg
    17641 actcgccatt agccagcaaa cgggcgattt gctcccgctc tttctctgtc agtttctgag
    17701 gtctgccgcc atgacgcccc tgagcacggg cagcagcaag acccgcgcgt gtacgttcaa
    17761 taatgagttc ccgctccatt tccgccagcg cgcccattat atggaaaaag aaacgtccca
    17821 tgggagtgga tgtatcaata ttgtccgtaa tgctgcggaa gttaacgccg cgttgccgga
    17881 gttcttctgt taaaaggaca aggtgctgca tactgcgtcc aaggcgatcc agtttccaga
    17941 ccagcagggt atcccctggc cggagtcgct ttaatgcgcg tttaagtccg ggacgctccc
    18001 ttgtcttacc gcttattctg tcttcgaaaa acagctcaca tcctgcacat tccagcgcat
    18061 ttctttgtag cgccgtattc tggtcatttg ttgacaccct gatataccca atcagcatac
    18121 gatttcctta ttataaaaag ccggaattgt cgcatgagga ataaaacaga gttaatcctg
    18181 gctttcctga aaacctcggt ttacgggaaa cggtaaacag ggcggcgaat gctctgccat
    18241 cggacggcac cgccgttgcc gcgaatagac tcgccacccc aagaaatatt aatggggttc
    18301 cctttgacgg gacgcaggac ataaacatca cttctggtat aacacagcag gatgcggatt
    18361 cccgatatat ccaggatatc aggctgggag cagagaacag gcaggtaatg tacggtgggg
    18421 catattactc tgcgtacggt aatgttatgg caggcctgca gattgacagt aaagttgatg
    18481 cttcaaacga ttttatagca ttcagacccc tgcaaaaatt agtcggaggt acatggatta
    18541 cggtatctca gttataaata gtcagtaaat aatatatttt aaaacagatt tatttccagg
    18601 aggattttat ggaactaaaa aatataaccc gttattatcc tgaaaatatg ccatatggta
    18661 ataatgttca gtatttccag agtgaagatg gtaaggattt ctacgaatcc ctgccattat
    18721 tcaccaaaaa atataaactt tgcataacac ctgacagcgg cgttatctgc tcaatatcac
    18781 aggacgcctc ggcgctgtat cctgcggggt tttctgtggt tgaggtcgat gaactccctg
    18841 aaggcacgga tatttcagga aactggaagt ttgataatgg cataatctcc cgtattccgg
    18901 ttaactacgc aagaaaactg gaagcaatgc gccagtcata tcttaatcag gcatatgaaa
    18961 aaattaatga ctggagaacg gagctacaac tgggcaccat cagcgatgaa gacagagcag
    19021 cacttaccca atggatggca tacatcagtc aggtgaagaa aatggaacta cccgccatta
    19081 aaacagaagc tgaatttaac gccattaaat ggcctgagca gccacagtaa taaagaaggc
    19141 cggaaggcct tctgaatatc attatatact tgcagccgtc acccatgctc catttaacag
    19201 atactgcaac ggccggtaat acacgccgcc gacgttatct gccgtattct taccgacatc
    19261 ctgtacttta attccggtaa gaacattgcc gccccccaga ttcagatccc agctttccca
    19321 tgttccgggc atatgtgcgg tcaccgaccc cagtcttatg gcggttactg ttcctgatga
    19381 gatagtgata tcctgcgtcc cgtcaaaggg aacaccatta attgtgtggg catttgccag
    19441 cctgttcgcg gcaacggcgg tgccgtccga tggcag
//
LOCUS       DAJTVE010000079        19407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000079 DAJTVE010000000
VERSION     DAJTVE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..245
                     /locus_tag="OO439_002624"
     CDS             <1..245
                     /locus_tag="OO439_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6316959.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OO439_002625"
     CDS             complement(296..1063)
                     /locus_tag="OO439_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6316960.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OO439_002626"
     CDS             1259..2167
                     /locus_tag="OO439_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6316961.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OO439_002627"
     CDS             2164..3426
                     /locus_tag="OO439_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6316962.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OO439_002628"
     CDS             3423..4061
                     /locus_tag="OO439_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6316963.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OO439_002629"
     CDS             4066..4842
                     /locus_tag="OO439_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6316964.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OO439_002630"
     CDS             4931..6295
                     /locus_tag="OO439_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6316965.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO439_002631"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO439_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6316966.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO439_002632"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO439_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6316967.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO439_002633"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO439_002633"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6316968.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO439_002634"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO439_002634"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6316969.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO439_002635"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO439_002635"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6316970.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO439_002636"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO439_002636"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6316971.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO439_002637"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO439_002637"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6316972.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO439_002638"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO439_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6316973.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OO439_002639"
     CDS             complement(12844..13167)
                     /locus_tag="OO439_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6316974.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO439_002640"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO439_002640"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6316975.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO439_002641"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO439_002641"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6316976.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO439_002642"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO439_002642"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6316977.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OO439_002643"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OO439_002643"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6316978.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO439_002644"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO439_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6316979.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO439_002645"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO439_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO439_002646"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO439_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6316980.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5231 c         4847 g         4737 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAJTVE010000080        19126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000080 DAJTVE010000000
VERSION     DAJTVE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            55..444
                     /gene="csgE"
                     /locus_tag="OO439_002647"
     CDS             55..444
                     /gene="csgE"
                     /locus_tag="OO439_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6316981.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            469..885
                     /gene="csgF"
                     /locus_tag="OO439_002648"
     CDS             469..885
                     /gene="csgF"
                     /locus_tag="OO439_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6316982.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            912..1745
                     /gene="csgG"
                     /locus_tag="OO439_002649"
     CDS             912..1745
                     /gene="csgG"
                     /locus_tag="OO439_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6316983.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1810..2301)
                     /locus_tag="OO439_002650"
     CDS             complement(1810..2301)
                     /locus_tag="OO439_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6316984.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2403..2957)
                     /gene="ycdY"
                     /locus_tag="OO439_002651"
     CDS             complement(2403..2957)
                     /gene="ycdY"
                     /locus_tag="OO439_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6316985.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2981..3718)
                     /gene="ycdX"
                     /locus_tag="OO439_002652"
     CDS             complement(2981..3718)
                     /gene="ycdX"
                     /locus_tag="OO439_002652"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6316986.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3773..4711)
                     /gene="ghrA"
                     /locus_tag="OO439_002653"
     CDS             complement(3773..4711)
                     /gene="ghrA"
                     /locus_tag="OO439_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6316987.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            4945..5032
                     /locus_tag="OO439_002654"
     tRNA            4945..5032
                     /locus_tag="OO439_002654"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4979..4981,aa:Ser,seq:gga)
     gene            5077..5213
                     /locus_tag="OO439_002655"
     ncRNA           5077..5213
                     /ncRNA_class="other"
                     /locus_tag="OO439_002655"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5304..5990)
                     /locus_tag="OO439_002656"
     CDS             complement(5304..5990)
                     /locus_tag="OO439_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6316988.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(6571..7359)
                     /gene="phoH"
                     /locus_tag="OO439_002657"
     CDS             complement(6571..7359)
                     /gene="phoH"
                     /locus_tag="OO439_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6316989.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7980..9251)
                     /gene="efeB"
                     /locus_tag="OO439_002658"
     CDS             complement(7980..9251)
                     /gene="efeB"
                     /locus_tag="OO439_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6316990.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9257..10384)
                     /gene="efeO"
                     /locus_tag="OO439_002659"
     CDS             complement(9257..10384)
                     /gene="efeO"
                     /locus_tag="OO439_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6316991.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10442..11272)
                     /locus_tag="OO439_002660"
     CDS             complement(10442..11272)
                     /locus_tag="OO439_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6316992.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     VVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11938..13446)
                     /gene="putP"
                     /locus_tag="OO439_002661"
     CDS             complement(11938..13446)
                     /gene="putP"
                     /locus_tag="OO439_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6316993.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            13868..17830
                     /gene="putA"
                     /locus_tag="OO439_002662"
     CDS             13868..17830
                     /gene="putA"
                     /locus_tag="OO439_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6316994.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17870..18508)
                     /gene="rutR"
                     /locus_tag="OO439_002663"
     CDS             complement(17870..18508)
                     /gene="rutR"
                     /locus_tag="OO439_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6316995.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18796..>19126
                     /locus_tag="OO439_002664"
     CDS             18796..>19126
                     /locus_tag="OO439_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6316996.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGG"
BASE COUNT         4841 a         5122 c         4662 g         4501 t
ORIGIN      
        1 aaaaccggac acaagcggtt tcctgggcaa acgataacct caggcgataa agccatgaaa
       61 cgttatttac gctggattgt ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc
      121 gttgaggtag aagtcccggg attgctaact gaccatactg tttcatctat tggccatgat
      181 ttttaccgag cctttagtga taaatgggaa agtgactata cgggtaactt aacgattaat
      241 gaaaggccca gtgcacgatg gggaagctgg atcactataa cggtcaatca ggacgttatt
      301 ttccagactt ttttatttcc gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg
      361 attcaaactg aagaagcact aaatcgtcgc cagataaatc aggcgttatt aagtacgggc
      421 gatttggcgc atgatgaatt ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa
      481 catgcagtag ttctactcat gcttatttcg ccattaagtt gggctggaac catgactttc
      541 cagttccgta atccaaactt tggtggtaac ccaaataatg gcgctttttt attaaatagc
      601 gctcaggccc aaaactctta taaagatccg agctataacg atgactttgg tattgaaaca
      661 ccctcagcgt tagataactt tactcaggcc atccagtcac aaattttagg tgggctactg
      721 tcgaatatta ataccggtaa accgggccgc atggtgacca acgattatat tgttgatatt
      781 gctaaccgcg atggtcaatt gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg
      841 accatccagg tttcgggttt acaaaataac tcaaccgatt tttaagcccc agcttcataa
      901 ggaaaataat catgcagcgc ttatttcttt tggttgccgt catgttactg agcggatgct
      961 taaccgcccc gcctaaagaa gccgccagac cgacattaat gcctcgagct cagagctaca
     1021 aagatttgac ccatctgcca gcgccgacgg gtaagatctt tgtttcggta tacaacattc
     1081 aggacgaaac cgggcaattt aaaccctacc cggcaagtaa cttctccact gctgttccgc
     1141 aaagcgccac ggcaatgctg gtcacggcat tgaaagattc tcgctggttt ataccgctgg
     1201 agcgtcagag tttacaaaac ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa
     1261 acggcacagt tgccattaat aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca
     1321 tggttgaagg ttcgattatc ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa
     1381 gatattttgg gatcggtgca gacacgcaat accagctcga tcagattgcc gtgaacctgc
     1441 gtgtcgtcaa tgtgagtacc ggtgagatcc tctcctcggt gaacaccagt aagacgatac
     1501 tttcctatga agttcaggcc ggggttttcc gctttattga ctaccagcgc ttgctggaag
     1561 gggaagtggg ttacacctca aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa
     1621 caggggtcat tttcctgatt aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca
     1681 aagcagaacg gcagaatgac attctggtta aataccgcca tatgtcggtt ccaccagaat
     1741 cctgacagat aaaaatgccg gaattaataa tccggcattt tactgtttaa gcgaaagcgt
     1801 gagtgcccct cacgctttat ttttgatttc ctgctcacgg tgagccgtct ttgcactacg
     1861 cgccgccagc cacaggccgc tgtttttcat tgcgtaacca aataccagcc caagcgccag
     1921 cgaaggtaac accagtttcc agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa
     1981 cgtccccgga acaaatgaaa gcagcagctg tttggcctga atacacatca gaaacgccac
     2041 aataccggtt atgacataac cgagaatttc cagatgtggt gccagtgcac taccgtaaat
     2101 aatgaccatc gcccacacca cgccactaag cagggttgca gcggagatcg ccagcccttt
     2161 cagcccacct tgcgggcagg caaagtaggc cgtacagccc aagaagcccg cccagcttag
     2221 taagcccagg gaaacagcca cccatcccca gataccggag agaatgcctg ttgtgattgc
     2281 aatagagaga agtatgttca tgccgcgcat gatagcagaa aacgcggcca tgaatgagat
     2341 cgagcacaca ttttatgcaa caacaaatta tctgttgcag ttatgagtga cgatgatcac
     2401 atttactctt cagaatcttc ttccagctcg tcccacattg cactaatggc atcacgggtt
     2461 agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt gccgaggaac
     2521 gcaccacacc agggtaacag atactcactg aacagtgttt ccagtgcttc gctctcatct
     2581 tccgttgact gatcttccag ccaggaagcc gcaagcagca atgtgccgat gtgatcggct
     2641 ggcgtatccg ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt
     2701 acgtcctcaa cccatgcgct acgatatggc ggcactgcac attcatcgcc gataaacaac
     2761 gcattgtaat cggcagagac ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac
     2821 tcatcctgct ccagtggcca gttcgcagcc agtttcccct cacgaatcag ggtaaacagc
     2881 ggcaccagta aaggatcttg cggttggcgg taatagagcg aacccagcac acgacagagg
     2941 atagaaaact cgttcattaa tatatttcca ttactagtaa ttaaaggtct gcaaattccg
     3001 caatcggtgc cataccgcga gattcaagga agttcagtaa gcggcgcgga gaaacattca
     3061 aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt aagacactct tcaaattccc
     3121 ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac ccaacctcca gcatcacgta
     3181 ccgctgcggc taccgcacga cagttgtctt cactgccctt acgtgagtgt aaaaatgaag
     3241 aattattgat ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg gctttcacat
     3301 ctatttcata tttgggattt ccgggatggc ttattatatg cacattgccg ctggcgatag
     3361 tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa
     3421 aaccggcaat aatcagatcc agcgagtcaa acattttacc gctgcaatca atttcaccat
     3481 caacattttt aatgttagct tcgatgccgc gcaggatccc taccccatca accactcgcg
     3541 gccagatacg catgttaatg aagtgccagt gatgcggagc atcttccata tccgggccat
     3601 gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg taatcactca
     3661 atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg ggatacataa
     3721 ctctctcctg tattcaattc ccgccaagga tagcaggaat cctgatgctt tattagtagc
     3781 cgcgtgcgcg gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc
     3841 gagaaatgta ctccacagct tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg
     3901 tcacgcgtgg atgttgccag agcggacttt caggcggtaa gggttcacga ttaaaaacat
     3961 ccaacattgc gcctttaact ttgccgctat ccagcgccgc gagcaggtca tcttccacaa
     4021 catgaacacc acgcgccagg ttgaggagat acgcgccatc cggtaatttt tcgagtaatt
     4081 gttgattaat aatgccgacg gtttcagggg tattcggtaa caaattaatc aatacccgac
     4141 attggctcag aaatgcagac agttcttccc gtccggcaaa gctttgcacg ccaggccacg
     4201 atttacgggt tcgactccag caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa
     4261 ctttactgcc cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt
     4321 caggcagcgg ttgccaatgc gaactatttt gctggatgcg ataatcgtca aaacgtcgaa
     4381 accaatgcag cacctgactg acagcatatt cctgcatttg ctcgcccata ccggtatctt
     4441 ccaggcgaaa aagtggaaca gaagggttca gcatttcagg gtgtgcctgt agcttgctca
     4501 aaatagaatc aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc cctgccagca
     4561 tttcaacagg aggatgccag actaaagcat aatcagcaga gtcattatct ccgcttttcc
     4621 atgctctgac tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac cattgggtat
     4681 cgaacgttgg gtgataaaag atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat
     4741 ttattcgcac ttatcgttat gatctacaaa ggccaccagc ataacaaatc cgtggtcggt
     4801 ggcaaaaaaa gcagatttcg cttattaaaa ccacacattg attgaagttt gaataaacgc
     4861 gcgatttttt caaaaagttt gttgacctca ggtcatgatt tccctaaatt agcgcccgtt
     4921 ccagcaagac aggaacgaca atttggtgag gtgtccgagt ggctgaagga gcacgcctgg
     4981 aaagtgtgta tacggcaacg tatcgggggt tcgaatcccc ccctcaccgc catatttaaa
     5041 gaagagctcg tacgaaagta cgggcttttt tttcgtatat tgcacacacc ggggggatga
     5101 gaagccccga ccggggttcg acaactggcg acagccagtt ggacagaccg tgaacgcagt
     5161 gagcgggctg cccgcagggc gagcgaagcg agtcaatccc cccctcaccg ccatctttaa
     5221 agaagagctc gtacgaaggt acgggctttt tttcgtatat tgcacacacc ggggggatga
     5281 gaagccccga ccgggatact tagttaatgc accggttcac tctttgtcgc cgcgactaat
     5341 gggcgaattt tgtcacgagt ctcccagacc ggaggaattt cctccaattt agtgacagaa
     5401 gctcgtacaa aaggcacccg tgcaagagtc ataattgtat ccaaccaacg gtcacgaata
     5461 atgcgatccg ccctttcatg actcgggtca tccagctcaa taacacagac tattttattg
     5521 ttcttctttg aaaccagaac ataatccatt gccatgccat tataaattgc aaagaaacgc
     5581 tttctttctt cttcgctaaa ctcaggttgt tctggaaagt tgatcaagcg ccccagagag
     5641 acctggcaat ggatatgaca ataatgtcca aaccattttt ccagattatc gaaatacatt
     5701 ttttcacaac gcgtgattaa ccactctttt ctttccaata aaataccttc ggcaaccatt
     5761 ccctgaaata actcatctcg ctcttgccaa cgagagtcac gataggtttt ctgcggtata
     5821 tagctccttg tcgacgcctt agcggcaggg gaagttgcag atgaagggac gggttgcgat
     5881 ttagcgatga ttaccgatga ggcaactttt cggagtgtca attttctggc aacagcaaaa
     5941 ccggcgataa atgcaattgt caaacaagtt acaaaagcca taaagctcat aattaatttt
     6001 cacctatcca tgttgattat atttcattac caatgtaact cgctgcagcg tgttacatgc
     6061 caacaatgca acgatcaata tatagttagt atcatgtttt gattattaca accatcccta
     6121 tttagccatt gcggcttgcc ttccacaaaa aaataacaac acacaatatt agcaaagaat
     6181 taattaagat taaccttaca tttataatat tatccctaaa gaataaatca gaggaatata
     6241 ttcggtgtta aacagtcagt caaagtatag cccattgttg attaaacctc ataagcaaaa
     6301 ttggcaccag atctcctgta caggttgaat ggccgaaaaa agataggcag atgctcattt
     6361 ttttggcttc tggaagaaaa actctccgcg ctggcagtat tacactggtc tgatgctatg
     6421 acgaaaaatg cctggtaaga gtatctaaac cagactccag ttcagcttat caacacgctc
     6481 aaagtcgccc ccctgaacgc taacctgaag tgaaaatctg ttgaaataaa acaaagcccg
     6541 gttcgcccgg gctctgcacc gataacacac ttaactgtag gcatgcagcg tacgttggca
     6601 aagtgccgaa cgtacgcagt cctctttacc gaaccggacg atcccaacca tttcatcttc
     6661 ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg ccgcgaggca aatcgcattg
     6721 cgtgatatca ccgttgacga taaccgtcac gttctccccg aggcgggtta aaaacatttt
     6781 catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt
     6841 acgtccacgc atataggcga acggcgcaat ttccaccttc cctatttccg gtcgcaggca
     6901 gtactgcata aaggaagccc ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata
     6961 gggagcaaac ttttctgcga tatctccagg taagaagcca agatcttcat cggcttgcag
     7021 aactggacgg gtgacgataa tcctgtcgac atccttatgt atcagggcct ctgccgcttt
     7081 tgctgcgctg atccaggttt ttccgccccc ggcttcgccc gtggcgaata tcagctgctt
     7141 actctcaata gccttcagat agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt
     7201 atcgcgactg tcgcgggcca taccaatggc ttctacgccg cccatctgca caagcgaggt
     7261 gaccgattct tcttcacgct gcttatggct gcgcgaatcc cgtctcagca cacgttttgc
     7321 ctcgcgacga gctttgatca ctgctttttg tcttcccata gagagcacct tgagttgttt
     7381 gtattcatca cacgcgccgt tggcagcgcg attatgcgca cgaacatcag agggttggct
     7441 tccttgtaag ccatagtttg cttttggata aaatgccgaa aaacggctac gcgcaccgtt
     7501 tacggcgtcg gtaacacatg aaaagaaagg atgaggttga aaatgcaaag tgacgagatg
     7561 actaccggag gagaaaactc cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt
     7621 acaggacgtt accatccgcg atctccatag tgactgacta tcactgccgg gaacttccgc
     7681 tgctacttaa taagtacaac agatctcgca tttattgcaa caatatattt acttatattt
     7741 aactataaaa cactatttca gtgacattag tttctactgg aaagatgaca gagtgatgac
     7801 agtgatgaaa aaagctgtgt gctttcagca ggatttgcat caggcatcag tgcacgatcg
     7861 ccggatgcgg cgtgaacgcc ttatccagcc taccaaaccc gcactgattg taggcatgat
     7921 aagacgcgca agcgtcgcat caggcatctg cgcacaaatg ccttatccgc ctaaaaacat
     7981 taaacccgca ataacgcgct tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa
     8041 aaaaaataac cgccgccgat aggtttaacg tattcctcca gcgcttcgcc attgagcctt
     8101 ttttgtactg tcaggaagcc tttttccaga tcgtgttggt agcagacaaa cagcaacccc
     8161 atatccagtt gtccggagtt ggtgaccccc aacgaataac tgtagccgcg acgcagcatc
     8221 aggctggact cactctccgc cgtgcgggga ttcgccagcc ggatatggct gtccagcgca
     8281 atcaccttcc cttccgggtc gctggcgtaa tcaggcacat catgctcatg ctgcattccc
     8341 agcggcgcac cggtttgctt atcacggcca aaaatcgtct gctgttcttt cagcggcgtt
     8401 ctgtcccaaa attccactcg aaactgaatc aagcgtactg cctgatagct gccaccgatt
     8461 gtccacgcag gctcctgctg atctgccgtt acccacacca ctttttgcat caacttatca
     8521 ttctggctat cgggattggc agtgccgtct ttgaaaccca gcaaattaat cggcgtctct
     8581 ttgcctttac tacgcgacac gtgatcggaa ataaaccctt cccgcttcca gcgcacactg
     8641 agcaaatccg gcgtgtgttt gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg
     8701 ttggcgcaaa tctgtagcaa cacatcacca tgacataacg ccgcatccag cgagtcgttg
     8761 gggaaacgcg tcatcttctg cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca
     8821 tcaaacaatg agtgacccac cgataacgtg atggtgagat tatcgggcgc aatgtagccg
     8881 ccaagaatgc cggaatcgag tggtggcagg cgcggatttg gcgtttctgg tgctgctccg
     8941 ccctgagtca gaaaagcaaa acgctgagtc aacaagcgaa acaaccgctc aagatcggct
     9001 ttatcgctgg caagcacatc aaacgccacc agcatcattg cggcctgttg tggcgtcagg
     9061 atccctgcct gatgctcacc ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc
     9121 gtgcccggtg cactttgcgt tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc
     9181 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     9241 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     9301 ccagcgcagt aatcggtcct ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg
     9361 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     9421 catcgacttt tgccagcagt tccgggttgg ctttttgcag ttgtggacgc agcaaatcga
     9481 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     9541 gatcttcttc accgctaatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     9601 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     9661 ctgtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     9721 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     9781 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatgc
     9841 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     9901 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     9961 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
    10021 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
    10081 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
    10141 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
    10201 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
    10261 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
    10321 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
    10381 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
    10441 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
    10501 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
    10561 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagagcact gcgctcatat
    10621 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
    10681 cagcagccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
    10741 caagattgag gcgaataccg ccccagtaga gcaggaagcc tagcaccacg acagtagcaa
    10801 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
    10861 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
    10921 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgctcc agttgtactt
    10981 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
    11041 tcaccgccac gataccttca aacagttcct gttctttttg cggaaattcg ccggtggttt
    11101 cgttaatgaa gatacccaag cccaggcaca acgcagcggc aagcaacacg ccaatccaca
    11161 tcacgccaat ccatcggcct cgctgggtac gcttaagata gctggcaatc aaactgacaa
    11221 tcagcgcggc ttcaagtcct tcgcgcaaca taatgagaaa cggaacaaac atgccaccca
    11281 cccttaaacg tttttctgag tcaatccatg caaagaaaag taaatcgcag tgatagcgat
    11341 tatcattacg gcaaagaaaa atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa
    11401 caaagagagg taataccagg ggatataaac gattattcca ggtacaaaga tgggattgtt
    11461 tacaacgagc catgtgttct tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac
    11521 tgtccagact caatgcatca cagcttccat gtaaaactga taagcgggtg tatcaagcaa
    11581 tttcgtccat ccgcgattat caaatctaac tttttgcccg gaacataata acagcgcggg
    11641 tgttggtggc gtcaaaatcg ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg
    11701 tgggctgagg gcgcgtctta caggacgtta cctcgcgccc gacccggtag ccgaagggga
    11761 taagctgaag gtaccgcgca gcggcgattt tgttcgccag agcccggggg tgcagggggc
    11821 ggcggcgact ggccgccccc tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac
    11881 attaagggag cgcaacccgc tgctgaagcc aaacaaaacc gccgcaggct aagtccctta
    11941 gttttcctgc aaccgtgacg gcggagccga atgatagtgc gcatcggcct cggcaaagcg
    12001 tttttgcatc gccgctgacg gcgctttacc cagcaaacta aacactacaa tcccgatact
    12061 gccgaagatg aagcccggaa taatttcgta cagtcccagc cagccgaact gcttccagac
    12121 gataactgtc agcgcaccga tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga
    12181 ccacatcacc gagaacagca ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta
    12241 gctcactaag cccagcacgc ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc
    12301 caccaccagc accatcacac gtcctaccca caccagctct ttctggctgg cctgtttacg
    12361 cagaaacgct ttgtataaat cttcggtaat cgcactggag cacaccagca gctggcaact
    12421 taaggttgac attaccgccg ccagaatcgc cgacagcaga atcccggcaa tccacgggtt
    12481 aaacagaatt tgcgccagtt cgataaacac gcgttcagcg ttctggttta ccgcacctgc
    12541 taccgccgga tgctcgttaa agtaagcgat accaaagaag ccaacagcca ccgcccctgc
    12601 caggcaaagg atcatccagg tcatactgat acgacgcgca tggacaatgc tgtggtgaga
    12661 atctgccgcc ataaaacgcg ccaggatgtg cggctgcccg aagtaaccca gcccccaacc
    12721 catcaatgag ataatggcaa caaagttcag ccctttgagc atatccacgt tttcgatgct
    12781 cttttgtttg atcacttcca gcgagtcacc aaagccaccg acactgataa tgacgataac
    12841 cggcgtcagg atcagggcaa aaatcatcag gctggcctgt acagtgtcag tccagctcac
    12901 cgcgaggaaa ccgccaataa aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc
    12961 cgtttcgtaa ctcatgccaa aggtactttc aaacagacgc gcgcctgcca caatgcccga
    13021 agcgcaataa atggtgaaga acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg
    13081 gcttttatct tcaaaacgcc cggtgaaata atccggcaat gttaaggcgt tattattgta
    13141 ttcggtatgc acacgcaacc gcccggccac cagcttccag ttgatccacg cgcctaatgt
    13201 caggccaatg gcgatccagc tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc
    13261 cattaacagc cagccgctca tatccgacgc acccgccgat aatgccgtca cgaatggccc
    13321 aagactacga ccgcccagaa tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa
    13381 cccaatcaat atcatgccaa agatatagac acaaaatgtc accaacatcg gtgtgctaat
    13441 agccatctaa agtctccaaa aaattattat cggcaatgtc gaaacttgcc gttatatctg
    13501 ccaccggaac ggggtaacag agtttatgtt ttaccagggc gaccgtatcc tgccggaagc
    13561 gctggttatt cacaatcgat ttaacacacc atttacatta aattttagtg ctcagcgaca
    13621 ctatttttca tcaggttgca ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt
    13681 aactgccgca gagaaaaagt ctgagttatt ttttaatcac tgtcatatcg atttctttta
    13741 ttaacatttc attcattttt aagcttgcta cgcaagtcac atttaacatg gttgcacaaa
    13801 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
    13861 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
    13921 taagtctgcc gcgacacgta tcgatcgcac accacactgg ttaattaagc aggcgatttt
    13981 ttcttatctc gaacaactgg aaaacagcga tactctgccg gagctacctg cgctgctttc
    14041 tggcgcggcc aatgagagcg atgaagcacc gactccggca gaggaaccac accagccatt
    14101 cctcgacttt gccgagcaaa tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc
    14161 ctatcgccgc ccggaaaccg aagcggtttc tatgctgctg gaacaagccc gcctgccgca
    14221 gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
    14281 aaaaaatgcc agtggtcgtg caggaatggt ccaggggtta ttgcaggagt tttcgctgtc
    14341 atcgcaggaa ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa
    14401 agccacccgc gacgcgttaa ttcgcgacaa aatcagcaac ggtaactggc agtcacacat
    14461 tggtcgtagc ccgtcactgt ttgttaatgc cgctacctgg gggctgctgt ttactggcaa
    14521 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
    14581 aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
    14641 gttcgtcacg ggtgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa
    14701 aggtttccgt tactcttacg atatgctggg cgaagctgca ctgactgccg cagacgcaca
    14761 ggcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg
    14821 tggcatctat gaaggtccgg gcatttcaat caaactgtcg gcgctgcatc cgcgctacag
    14881 ccgcgcccag tatgaccggg taatggaaga gctttacccg cgtctgaaat cactcaccct
    14941 gctggcgcgt cagtatgata ttggtattaa catcgatgcc gaagaggccg atcgtctgga
    15001 gatctccctc gatctgctgg aaaatctctg tttcgagccg gaactggcag gctggaacgg
    15061 catcggtttt gttattcagg cttatcaaaa acgctgcccg ttggtgatcg attacctggt
    15121 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
    15181 ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg
    15241 caaggtgtat accgacgttt cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa
    15301 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact
    15361 ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
    15421 gccactgtac gagcaggtca ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg
    15481 tatttatgcc ccggttggca cacatgaaac gctgctggcg tatctggtgc gtcgcctgct
    15541 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
    15601 tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
    15661 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
    15721 ctcggcaggg ctggatctcg ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct
    15781 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg
    15841 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga
    15901 agccacgcca cgtgaagtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
    15961 gtttgccacg cctccggctg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
    16021 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
    16081 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactacgccg gacaggtgcg
    16141 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg
    16201 gaacttcccg ctggctattt tcaccgggca gatcgctgcc gcactggcgg caggtaacag
    16261 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
    16321 gctggaagcg ggtgtacccc ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt
    16381 gggcgcgcaa ctgacgggtg atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga
    16441 agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat
    16501 tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
    16561 cgaacaagtc gtcgtggatg tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc
    16621 ggcactgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg
    16681 cggtgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
    16741 ggtgattgat agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa
    16801 aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag
    16861 cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat tgcacaaaga
    16921 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat
    16981 cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga
    17041 aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat
    17101 ggtgggcgca gtggttggtg tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc
    17161 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
    17221 ggcagtgacg ctcgcgcgtc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc
    17281 attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc
    17341 gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc
    17401 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga
    17461 tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact
    17521 gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga
    17581 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
    17641 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc
    17701 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
    17761 tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac
    17821 gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
    17881 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat
    17941 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag
    18001 tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt
    18061 tgacccaacc ggcaatcagc gcagatttct catcaattaa tgccttcaaa tcgcccgtca
    18121 gttcatccat taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct
    18181 gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg
    18241 gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
    18301 gcacggcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
    18361 cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
    18421 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac
    18481 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
    18541 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt
    18601 tatgtgcaac tgttttgacc gtttagtcca ctttttatca gatattttag ataacgtaat
    18661 attaacatat taataacatt aactttttaa aaactggcat ccgctttgca aacaagccaa
    18721 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
    18781 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
    18841 atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcag
    18901 aaagcggagc attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
    18961 ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca
    19021 gccgtgacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca
    19081 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggc
//
LOCUS       DAJTVE010000081        18939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000081 DAJTVE010000000
VERSION     DAJTVE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..433)
                     /locus_tag="OO439_002665"
     CDS             complement(<1..433)
                     /locus_tag="OO439_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6316997.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQG"
     gene            complement(626..695)
                     /locus_tag="OO439_002666"
     tRNA            complement(626..695)
                     /locus_tag="OO439_002666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Gly,seq:ccc)
     gene            complement(774..1529)
                     /locus_tag="OO439_002667"
     CDS             complement(774..1529)
                     /locus_tag="OO439_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6316998.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1944..4241
                     /gene="xdhA"
                     /locus_tag="OO439_002668"
     CDS             1944..4241
                     /gene="xdhA"
                     /locus_tag="OO439_002668"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6316999.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4252..5130
                     /gene="xdhB"
                     /locus_tag="OO439_002669"
     CDS             4252..5130
                     /gene="xdhB"
                     /locus_tag="OO439_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6317000.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5127..5606
                     /gene="xdhC"
                     /locus_tag="OO439_002670"
     CDS             5127..5606
                     /gene="xdhC"
                     /locus_tag="OO439_002670"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6317001.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(5646..7424)
                     /locus_tag="OO439_002671"
     CDS             complement(5646..7424)
                     /locus_tag="OO439_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6317002.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            7903..9090
                     /gene="ygeW"
                     /locus_tag="OO439_002672"
     CDS             7903..9090
                     /gene="ygeW"
                     /locus_tag="OO439_002672"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6317003.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9148..10344
                     /gene="dpaL"
                     /locus_tag="OO439_002673"
     CDS             9148..10344
                     /gene="dpaL"
                     /locus_tag="OO439_002673"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6317004.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            10402..11613
                     /locus_tag="OO439_002674"
     CDS             10402..11613
                     /locus_tag="OO439_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6317005.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            11666..13050
                     /gene="hyuA"
                     /locus_tag="OO439_002675"
                     /pseudo
     CDS             11666..13050
                     /gene="hyuA"
                     /locus_tag="OO439_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13098..14030
                     /gene="arcC"
                     /locus_tag="OO439_002676"
     CDS             13098..14030
                     /gene="arcC"
                     /locus_tag="OO439_002676"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6317006.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14071..15696)
                     /locus_tag="OO439_002677"
     CDS             complement(14071..15696)
                     /locus_tag="OO439_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6317007.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(15744..16514)
                     /gene="yqeC"
                     /locus_tag="OO439_002678"
     CDS             complement(15744..16514)
                     /gene="yqeC"
                     /locus_tag="OO439_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6317008.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            16617..17195
                     /gene="mocA"
                     /locus_tag="OO439_002679"
     CDS             16617..17195
                     /gene="mocA"
                     /locus_tag="OO439_002679"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6317009.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            17517..>18939
                     /gene="ygfK"
                     /locus_tag="OO439_002680"
     CDS             17517..>18939
                     /gene="ygfK"
                     /locus_tag="OO439_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR6317010.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYI"
BASE COUNT         4929 a         4888 c         4501 g         4621 t
ORIGIN      
        1 ttccctgatc tgatactaat gccatatagc cccataaact atccctcagc cagacagcat
       61 catagtgggt ttctgccgct gttttgtcat cttcgaaagt agaagcaaga ataaatgttc
      121 cccagggttt ctccgtcaac gttaactttc gtgtgttttc tgttaatgtt aacaaccctt
      181 gaatttcgtc tttggtatag tggctcttta tttcattttt taatttggct gagtgaatca
      241 gaagacgata attagggtac ttatctggat ttaacgataa ataactttta tctgttgctg
      301 cccccgtgga attaagaatg ttaaatacag attcactcgt cacttctgcc cctgtcgcct
      361 ggcatgtgcc gcacgaaaat ataccagcga gcagaagagt taatgcagat ttccctttca
      421 taaaatattc catcaatcgt ttgttaaaag cgtaaataac cacttattta acaacttgaa
      481 aataaggtgt tttacctggg ttgttacaaa aggattgcat tgcgtaaacg ctttttattt
      541 acaacaaaat ggggaaatat tacggcgatt gcgaaaagtc tgaagcgatt gaattaagat
      601 aatatagaaa atcagataca gcaaaagcgg cgaagggaat cgaaccctcg tatagagctt
      661 gggaagctct cgttctacca ttgaactacg cccgcttcga gatgcgtaag gcattataaa
      721 ccttacgctc tccttagcaa gtgccgcgct gctgactgct gattaattcg ccatcagcat
      781 tttggcttgc tgccctgcgg cggcaagtaa cgtagcggat caattgccgt tgcacggtaa
      841 cgaatctgga aatgcaggcg aacagatgcc gcatccgtgc tccccatagt ggcgattttt
      901 tgcccagcct tcacgctttg cccattattt accagcatcg tgtcattatg ggcgtaagcc
      961 gtaatgtaat cttcactgtg tttaatcatg atgagattac cgtagccacg cagctggttg
     1021 cccacataca ccacctttcc tgcacccgcg gcgtaaatag gtgtaccccg tggagctgag
     1081 atatcaatcc ctttattgcc gccatctgct gtcgaatacg gcatgataac tttccctgtc
     1141 gttggccata accaacaacg ttgccctact ggcggccagg atgatttcgg taccgctgat
     1201 gacggtgtaa ccgatgcggt tttggtcgtt gatttggcgg ttgatttacg tgtactactg
     1261 ctacttttcg ccccacccag ttttagtttc tgaccaactt caatggtgta agggggggaa
     1321 atgccgttca gtcgcgccag ttcttttacg ctggttcccg tggtgcgcga aatacgatat
     1381 agcgtatccc cccgtttcac ggtgtaaacg gagccggaat acgttcctgt atcggatgat
     1441 ttgctacccg aacagcccgc caaaagcagt ccaaccgata acaacatcac gatacccaga
     1501 gattttttat tcaggcgtcc cgcactcaaa atctttcctc gctaaaaaaa caagacgcct
     1561 tatcatagca gccagcctct tgatacccaa tctcttgctt ctggaaaaac gtggtaatag
     1621 agaaagaggt taatttcatt tggcaaaggg aagtatcatc agcaaaatat tcgctgtaga
     1681 ggtatcgaac ttcctgactt tttaccctat ttcccatcct gatgcgtaat aatcccaatt
     1741 tatcacattg attttatcgc cactcatatt gatacgtatc actgccccta ttcagagtcc
     1801 tgtttcgtat catatctgtg tttttttaac gtcatcacac ttcctacctc ccctaacgct
     1861 tatcgtcgtt tctggcgtaa atcttgcctg cttagactaa atctttgcca taagaaccaa
     1921 atgttcaaat cgaggggatt tctatggaag cgcgggaagc aaccgctacg ggtgaatcat
     1981 gcatgcgcgt cgatgccatt gctaaggtca ccgggcgggc acgatatact gacgattatg
     2041 ttatggcggg catgtgttat gcgaaatatg tacgtagccc tatcgcacat ggttatgccg
     2101 taagtattaa tgatgaacaa gccagaagtt tgccgggcgt actggcgatt tttacctggg
     2161 aagatgtgcc tgatattcca ttcgctacag ctgggcatgc ctggacactt gacgaaaaca
     2221 agcgcgatac cgccgatcgc gcactgctaa ctcgccatgt tcgtcatcat ggtgacgccg
     2281 ttgccatcgt cgtggcccgc gatgaactca cggcagaaaa agcggcgcaa ttggtcagca
     2341 ttgagtggca agaattaccc gttatcacca cgccagaagc ggcgctggca gaagacgctg
     2401 caccaatcca taacggtggc aatttactga aacaaagcac gatgtcgacg ggtaatgtcc
     2461 aacaaacaat cgatgccgcc gactaccagg tacaggggca ctatcagacc cccgttattc
     2521 aacattgtca catggaaagc gtaacatcgc tggcgtggat ggaggatgac tcgcgaatta
     2581 ccatcgtttc cagcacccag atcccgcaca ttgttcgccg cgtggttggt caggcgctgg
     2641 atattccctg gtcatgcgta cgagtcatca aaccgtttgt cggtggcggt tttggtaata
     2701 aacaggatgt actggaagag ccaatggcag cattcctgac cagcaagctt ggcggcattc
     2761 cggtgaaagt ttcccttagc cgtgaagagt gtttcctcgc aacccgtacc cgccacgctt
     2821 ttaccattga cgggcaaatg ggcgtgaacc gcgacggaac attgaaaggt tatagtctgg
     2881 atgttctgtc taacaccggc gcttatgcat ctcacgggca ctccatcgct tctgcgggga
     2941 ggaataaagt cgcttacctt tatcctcgtt gtgcctacgc ttacagttca aagacctgct
     3001 ataccaacct cccctcggct ggtgcgatgc gtggttatgg cgcgccacaa gtcgtatttg
     3061 ccgttgagtc tatgcttgat gacgccgcga cagcgttagg tattgatcct gttgaaattc
     3121 gtttacgcaa cgccgcccgc gaaggagatg ctaatccgct cacgggcaaa cgtatttaca
     3181 gcgcagggtt gccggagtgt cttgaaaaag gccggaaaat ctttgaatgg gaaaaacgcc
     3241 gtgcagaatg ccagaaccag caaggcaatt tgcgccgcgg cgttggcgtc gcctgtttta
     3301 gctatacctc taacacctgg cctgtcggcg tagaaatagc aggcgcgcgc cttctgatga
     3361 atcaggatgg aaccatcaac gtgcaaagcg gcgcgacgga aatcggtcag ggtgccgaca
     3421 ccgtcttctc gcaaatggtg gcagaaaccg tgggggttcc ggtcagcgac gttcgcgtta
     3481 tttcaactca agataccgac gttacgccgt tcgctcccgg cgcatttgcc tcacgccaga
     3541 gctatgttgc cgcgcctgcg ctgcgcagtg cggcactatt attaaaagag aaaatcatcg
     3601 ctcacgccgc agtcatgcta catcagtcag cgatgaatct gaccctgata aaaggccata
     3661 tcgtgctggt tgaacgaccg gaagagccgt taatgtcgtt aaaagatttg gcgatggacg
     3721 ctttctacca ccctgaacgc ggcgggcagc tctctgctga aagctccatc aaaaccacca
     3781 ctaacccatc agcgtttggc tgtacctttg ttgatctgac ggtcgatatt gcgctgtgca
     3841 aagtcaccat caaccgcatc ctcaacgttc atgattcagg gcatattctt aatccactgc
     3901 tggcagaagg tcaggtacac ggcggaatgg gaatgggcat tggctgggcg ctatttgaag
     3961 agatgatcat cgatgctaaa agcggcgtgg tccgtaaccc caatctgctg gattacaaaa
     4021 tgccgaccat gccggatctg ccacaactgg aaagcgcgtt cgtcgaaatc aatgagccgc
     4081 aatccgcata cggacataag tcactgggtg agccaccaat aattcctgtt gccgctgcta
     4141 ttcgtaacgc ggtgaagatg gctaccggtg ttgcaatcaa tacactgccg ctgacgccaa
     4201 aacggttata tgaagagttc catctggcag gattgatttg aggataacat catgtttgat
     4261 tttgcttctt accatcgcgc agcaaccctt gccgatgcca tcaacctgct ggctgacaac
     4321 ccgcaggcca aactgctcgc cgatggcact gacgtactga ttcagctcca ccatcacaat
     4381 gaccgttatc gccatattgt tgatattcat aatctggcgg agctgcgggg aattacgctg
     4441 gcggaagatg gctcgctacg tatcggctct gcaacgacat ttacccagct aatagaagct
     4501 cctataactc aacgtcatct cccggcgtta tgtgctgcgg ccacgtccat tgctggaccg
     4561 cagatccgta acgtcgctac ctacggtgga aatatttgca acggtgccac cagcgcagat
     4621 tctgccacgc caacgctaat ttatgacgcg aaactggaga tccactcccc gcgcggtgtt
     4681 cgtttcgtcc cgattaatgg ctttcacacc gggccgggca aagtgtctct tgagcatgac
     4741 gaaatcctcg tcgcctttca ttttccgcca cagccgaaag aacacgcggg cagcgcgcat
     4801 tttaaatatg ccatgcgcga cgcaatggat atttcaacga ttggcagcgc cgcacattgc
     4861 cgactggata acggcaattt cagcgaatta cgcctggcat ttggtgttgc cgcgccaacg
     4921 ccgattcgct gccaacatgc cgaacagact gcacaaaatg cgccattaaa cctgcaaacg
     4981 ctggaagcta tcagcgaatc tgtcctgcaa gatgtcgccc cgcgttcttc atggcgggcc
     5041 agtaaagagt ttcgtctgca tctcatccag acgatgacca aaaaagtgat tagcgaagcc
     5101 gtcgccgcgg cggggggaaa attgcaatga atcacagcga aacaattacc atcgaatgca
     5161 ccattaacgg gatgcctttt cagcttcacg ccgcaccagg cacgccgctc tcggaattac
     5221 tccgcgaaca aggactgcta agtgtcaaac aagggtgctg cgtgggtgaa tgtggtgcct
     5281 gtacggtgtt ggtcgacggc acagcaatag acagttgctt ataccttgcc gcctgggctg
     5341 aaggaaagga gatccgcacg ctggaaggtg aagcgaaagg cggaaaactt tctcatgttc
     5401 agcaggctta tgcgaaatcc ggcgcagtgc agtgcgggtt ttgtacgcct ggcctgatta
     5461 tggctaccac ggcaatgctg gcgaaaccac gcgagaagcc attaaccatt acggaaattc
     5521 gtcgcggact ggcgggaaat ctttgtcgct gcacggggta tcagatgatt gtaaatacag
     5581 ttctggattg cgagaaaacg aagtaaaagg atatccggcc tgaattcagg ccggattcac
     5641 tgaggttatg tgtttaacaa ctcatatttc ttaatcttgc gatagagcgt agcaatgccg
     5701 atgcccagtt catcagcaac ttgcttcttg ctgttatgac gtgaaagcgc ctcgcggatc
     5761 atttgctttt ccatctcctc cagcgccgtg ccgcccgcat catcgagtga caggtgcgcc
     5821 tcactgacct ctgttacatc actttgctcc gttgtgccat tattcagcag atttggcggc
     5881 aatagcgtgc tgtcgataac ttcacctgaa ggaaccacgt taaccagata ttccatcaaa
     5941 ttgcttaact cgcgcaggtt tccgggccaa cgatgcttac gcaatatttc gacgacatcg
     6001 ggagcaatgc caggataaac cgatcccaga cgacgggtat gcagatgtaa aaagtaatgc
     6061 accaatagtt caatatcttc ctgacgttca cgcagcggtg gcagagttat cgggataaca
     6121 ttaagtcggt agaagagatc ttcgcggaat ttaccttcgg caatgaactg ggccaaattc
     6181 tgattagttg cagaaatgat gcgaatgtcg acttgtattg ggctactggc accaatcggc
     6241 agaatttcac gcgcctcaat agcgcgcagt aatttagcct gcaacattaa tggcatatca
     6301 cctatttcat cgagaaacag cgtgcccgta ttcgccgcct gaatcaaccc tgttttaccg
     6361 ttggcagaag caccagtaaa tgcgccttta acataaccga acagttcgct ttccagaagc
     6421 tgctccggaa tcgcggcaca gttgatagca ataaagggtt tattccgtct tccgctcaac
     6481 ttatggattg ctcgggcgac gacttcttta cccgtgccgc tttcaccaac caccataacg
     6541 ctggatgggc tgggtgcaat acggctaatg agtcgtttta attgccgcat aacacggcac
     6601 tcgccaacca attgttcaat atgtggttca tcaggtgcat ttgctacaga aaaactggta
     6661 tgcgattggt gaaatgccat taaaaataat tgtcggccct gaatgttatg caattgacca
     6721 atgattaatt cacttttatc gtcccacgaa acaatatgct gcatatgtcc atgagtaaaa
     6781 ttactctcaa atgttaatgg tctgaaacgg ataggtttcc caataatatt attttgtaca
     6841 acaccaagtg tttttaaggc agtctgatta acaaactgaa cccgactttc atcatcaaca
     6901 actaatacgc cctgatccat attatcgatc atggtcgcga atattttact gatgttatct
     6961 cctggcccct gatcctccag aagtttcgaa acaaaaatgg tggatatatg gcgaacataa
     7021 tcagaaaatt cgcgtaaatt atcactgata tgctcttgtt gctcgtgggt aacggcaatc
     7081 aaacttatca ccccaacaca acgatcctgt aaaatgacag gcgtacccag aaatgctttt
     7141 tcgcggcaat tttctttact atcgcaacct tcgcaaaggg gatcgaagcg agactgtgtc
     7201 acaacttttt cagttttcgt ttccaggacg tggcggagca ggcgtgagtt gccgctcaac
     7261 tggcgaccaa gaaacttccc atacgcgccc gttccggcga cgcgacacaa gttttcatca
     7321 acgatctcaa cctcaaactg caaaacgctg gcaagcattc tggcaaaacg ctgaattgtc
     7381 ggttgaattt gcatcaatac tgactgcgta gtagcaagct ccatagcttt accttccaga
     7441 cttacttaaa agtcgatcat tgaagacgtt gatggttcac agatcatgat gatattaact
     7501 caggcgaaat tggctttgat aaaaacataa gatttttatc attttctaat gcaattatgg
     7561 aagagatatc acatttctat atcaatatga gaattacggc ggtgagttta tcaaactgaa
     7621 gagagatagc ctgccccttt atcttatttc tgatacttag cagaaaataa ataacgcgat
     7681 aaaaaaagcc aaacgttttc gtattttaca aacaaccaga agctggcatc aatttgtgaa
     7741 caaccccaca cattatccgt caaattagtc ttttgcagcc gcgcggataa ttctggcaca
     7801 cttattgtta gtcccaggta tagctatgaa aacaccaatc actttggcaa gtcacagtga
     7861 aataaaccac tttgcctgtc attccactac cgggactgta tgatgaaaac tgttaatgag
     7921 ctgattaagg atatcaattc gctgacctct caccttcacg agaaagattt tttgttaacg
     7981 tgggaacaga cgccagatga actgaaacaa gtactggacg ttgccgcagc attaaaagca
     8041 ctgcgtgctg aaaacatctc aaccaaagtc tttaatagtg gattaggtat ttccgtattc
     8101 cgcgacaact ccacccgtac ccgcttctct tatgcttccg cgcttaacct gctcggcctt
     8161 gcacaacaag atctcgacga aggcaaatca caaatcgctc acggcgaaac cgtgcgtgaa
     8221 accgccaata tgatctcctt ctgcgccgac gctattggta ttcgcgacga tatgtatctg
     8281 ggcgcaggca acgcctatat gcgtgaagtt ggcgctgcac ttgatgacgg ttacaagcag
     8341 ggtgtactgc cacagcgtcc ggctttagtg aacctgcaat gcgatattga ccacccgact
     8401 cagtcaatgg ctgacctcgc gtggttacgt gaacactttg gttcactgga aaacctgaaa
     8461 ggtaaaaaaa tcgccatgac ctgggcctac tctccaagct atggcaaacc gctctctgta
     8521 ccacaaggca tcatcggtct gatgactcgc ttcggtatgg atgtcacctt ggcccatccg
     8581 gaaggctacg acctgatccc ggatgtggtt gaagtggcga aaaacaatgc taaagcctcc
     8641 ggtggtagct tccgtcaggt caccagcatg gaagaagcct tcaaagacgc agacatcgtt
     8701 tatccgaagt catgggcacc ttacaaagtg atggaagagc gtactgaatt gctgcgtgcg
     8761 aacgaccacg aaggcttaaa agcactggaa aaacagtgtc tggcacagaa cgcgcaacac
     8821 aaagactggc attgtactga agagatgatg gaactgaccc gtgatggcga agccctgtac
     8881 atgcactgcc tgccagctga tatcagcggc gtatcctgca aagaaggtga agtgactgaa
     8941 ggcgtattcg aaaaataccg tatcgctacc tacaaagaag ccagctggaa gccttatatc
     9001 atcgccgcga tgatcctgtc ccgtaaatac gccaaaccag gtgcactgct cgagcaactg
     9061 ctgaaagaag cgcaagaacg cgtgaaataa cccctcgggc cgatctgctg gtcggccctc
     9121 tatttccaga ggccaaaagg ataggatatg tccgttttct cattgaagat tgatatcgcc
     9181 gataacaaat ttttcaacgg cgaaacatca ccgctctttt cgcaaagcca ggccaaactg
     9241 gcgcgccagt tccaccagaa aatagctggt tatcgcccaa caccgctttg tgcgctggac
     9301 gatctcgcaa acctttttgg tgtgaagaaa attctcgtca aagacgaatc aaaacgattc
     9361 ggtctgaacg ccttcaaaat gcttggcggt gcgtacgcca tcgctcaatt attgtgtgaa
     9421 aaatatcatc ttgatatcga aacgctgtca tttgagcacc tgaaaaatgc catcggcgaa
     9481 aaaatgactt tcgcgaccac caccgacggc aaccacgggc gcggtgtggc gtgggcagca
     9541 cagcaactcg gacagaatgc ggtgatttac atgccgaaag gttctgctca ggaacgcgtt
     9601 gacgccattc tgaacctcgg tgccgagtgc atcgtcacgg atatgaacta tgacgatacc
     9661 gttcgcctga ccatgcaaca cgcgcagcag cacggctggg aagtggtaca ggacacggca
     9721 tgggaaggtt acaccaaaat cccaacctgg atcatgcaag gctacgcaac cctggcagat
     9781 gaagccgtcg agcaaatgcg tgaaatgggc gtaaccccga cgcacgttct gctgcaagcc
     9841 ggtgtcggag caatggccgg tggtgtgctg ggttatctgg tcgacgtcta cagcccgcaa
     9901 aatctgcaca gcattattgt tgaacctgac aaagctgact gtatttatcg ctccggcgtc
     9961 aaaggcgaca tcgtcaacgt tggcggtgat atggccacca tcatggcggg cctggcctgt
    10021 ggcgaaccta acccgctggg ctgggaaatc ctacgtaact gcgccaccca attcatctcc
    10081 tgccaggaca gcgttgccgc attaggtatg cgcgtgctgg gtaatccgta cggcaacgac
    10141 ccgcgcatca tctccggtga atccggcgct gtcggtttgg gcgttctcgc agcggttcat
    10201 tatcacccgc aacatcaaag cctgatggaa aaactggcgc tggacaaaga tgccgtagtg
    10261 ctggttatca gcactgaagg cgacaccgac gtgaagcact accgcgaagt tgtctgggaa
    10321 ggcaaacacg ctgtagcacc ttaatcaccc tattggaact actccctgca aaacggggag
    10381 taaaaaatct ggagaaaaat aatggctaag aatattccat tcaaactgat tcttgaaaaa
    10441 gcaaaagatt accaggcgga catgactcgc ttcctgcgcg acatggttgc tattcccagt
    10501 gaaagctgcg acgaaaaacg cgtagtacat cgtattaaag aagagatgga aaaagtcggc
    10561 ttcgataaag ttgaaatcga cccgatgggc aacgttctcg gttatatcgg ccacggcccg
    10621 cgtctggtgg caatggacgc tcatatcgat accgtcggca ttggcaacat caaaaactgg
    10681 gacttcgatc cgtacgaagg catggaaact gatgagctaa tcggtggtcg cggtacttcc
    10741 gaccaggaag gcggcatggc atctatggtt tatgccggta aaatcattaa agacctcggt
    10801 ctggaagatg aatataccct gctggttacc ggtactgtgc aggaagaaga ctgcgacggt
    10861 ctgtgctggc agtacattat tgaacaatcc ggcattcgcc cggaatttgt ggtcagtacc
    10921 gaaccaaccg actgccagat ataccgtggt cagcgcggtc gtatggaaat tcgtattgat
    10981 gttcagggtg ttagctgcca cggttctgcg ccagaacgcg gtgacaacgc cattttcaaa
    11041 atgggtccga ttcttggcga attacaagaa ctctcccaac gtctgggtta tgacgaattc
    11101 ctcggcaaag gcaccctcac cgtttctgaa atcttcttca catccccaag ccgttgcgct
    11161 gtagcagata gctgcgccgt ctctattgac cgccgtctga cctggggcga aacctgggaa
    11221 ggcgcgctgg acgaaatccg cgccctgcct gcagtacaga aagctaacgc ggttgtttct
    11281 atgtacaact acgaccgtcc gtcctggact ggcctggttt acccaaccga atgctacttc
    11341 ccgacctgga aagtggaaga agatcacttc accgttaaag cactggtgaa tgcctacgaa
    11401 gggctgtttg gcaaagcgcc ggttgttgat aagtggacct tctcaactaa cggcgtatct
    11461 atcatgggcc gtcacggcat tccggtgatc ggctttggcc cgggtaaaga acctgaagcg
    11521 catgcaccta acgaaaaaac ctggaaatct cacctggtga cctgtgccgc gatgtacgct
    11581 gcaatcccgt taagctggct ggcaacagag taattactct tcacttattc ccctccggtt
    11641 cgccggaggg tttttggagt ttgctatgcg cgtattgatc aaaaacggca ctgtcgttaa
    11701 cgcagatgga caagccaaac aggatttgct gattgaaagc gggattgttc gccagttggg
    11761 caacaatatt tcgccgcagc tcccgtatga agaaattgat gccactggct gttacgtttt
    11821 ccctggcggc gtggatgtcc atacgcattt caatattgat gtcggcatcg cgcgcagttg
    11881 tgatgatttt ttaccggtac ccgcgcagct gcgtgtggcg gtacaacaac cattattgac
    11941 catatgggat ttggcccaaa cggctgtcgg ttacgccatc aactggaggt ttatcgtggt
    12001 tatgccgccc ataaagcggt catcgattac agctttcacg gtgtgatcca gcacattaat
    12061 cacgcaatcc tcgacgaaat cccgatgatg gtcgaggaag gactgagcag ttttaaactc
    12121 tatttaacct atcaatacaa actcaacgat gacgaggttt tgcaggcatt acgccgcttg
    12181 catgaatccg gcgcgctgac caccgtgcac ccggaaaatg atgcggctat cgccagcaag
    12241 cgggcggaat ttatcgccgc agggttaacc gcgccgcgct atcatgcctt gagtcgccct
    12301 ctggaatgcg aagcggaagc catcgcccgc atgattaacc tggcacaaat tgccggtaac
    12361 gccccgctct atatcgtgca cctgtctaac ggcttaggtc tggattatct gcgtcttgcc
    12421 cgtgcgaatc accagccagt ctgggttgaa acctgcccac aatatctcct gttggacgaa
    12481 cgcagttacg atacagaaga tggcatgaag ttcattctta gcccaccgct gcgtaacgta
    12541 cgcgagcagg acaaactgtg gtgtggcatc agcgatggtg cgattgacgt ggtggcgacc
    12601 gatcactgca ccttctcgat ggctcaacgc ctgcaaattt ctaaaggcga tttcagtcgc
    12661 tgcccaaatg gcttacccgg tgtggaaaac cgcatgcagt tactgttttc cagtggcgtg
    12721 atgacgggac gtataacacc ggaacgcttt gttgaattaa ccagcgcaat gcccgccagg
    12781 ctgtttggcc tgtggccgca aaaaggatta ttagcgcccg gttccgatgg cgacgtggtg
    12841 attatcgacc cacgtcagag ccaacaaatt cagcatcgcc atctccacga caacgccgac
    12901 tactcgccat gggagggttt tacctgtcag ggcgcgattg tcagaacctt atcccgtggt
    12961 gaaacgattt tctgtgacgg cacctttaca ggcaaagccg ggcgaggtcg tttcctgcga
    13021 cgcaaaccgt ttgtccctcc cgtgctctaa ccactgtcag ggatctatac acaatcaaga
    13081 atgatgagtg aggaaaaatg agtaagaaaa ttgttctcgc cctgggcggg aatgcgctgg
    13141 gcgatgacct ggccggacaa atgaaagcgg taaaaattac ttctcaggca attgttgatt
    13201 taattgccca aggacatgaa gttatcgtca ctcatggtaa cgggccgcag gtgggcatga
    13261 ttaaccaagc ctttgaagct gccgcaaaaa ctgaagcaca ctcgccaatg ctgccgatgt
    13321 ctgtctgtgt tgccttaagc cagggttata ttggttacga tctacaaaac gcgttaagag
    13381 aagaactgct ttctcggggc attaataaac ctgtagcaac gctggttact caggtcgaag
    13441 tcgacgctaa cgatccggca ttcctcaacc cgaccaagcc gatcggttcg ttctttaccg
    13501 agcaagaagc agagcaactg acaaaacagg gttacactct gaaagaagat gctggtcgcg
    13561 gctatcgccg tgttgttgcc tcgccaaaac cggttgatat cattgaaaaa gaaacggtta
    13621 aagctctggt agatgcaggc caggtggtga tcgccgttgg cggtggtggt atccctgtta
    13681 ttcgtgaagg taaccatctg cgtggtgcca gcgcggttat cgataaagac tgggccagcg
    13741 cccgtttagc agaaatgatt gatgccgata tgctgatcat tctgactgcg gtagaaaaag
    13801 tggccattaa cttcggtaaa gagaatgaac agtggctcga tcgcctgtca ttaagtgatg
    13861 ctgaacgctt tatcgaagaa ggtcattttg cgaaaggttc tatgctgcct aaagtggaag
    13921 ctgccgcctc gtttgctcgc tctcgcgcag gtcgtgaagc actgattacc gtattgagca
    13981 aagcgaaaga agggattgaa ggcaaaaccg gaacggtgat ttgtcagtaa ttttaaggcc
    14041 gggtgggtaa taccatccgg ccattttcat ttaagccact tccagcactt cttttgtcgc
    14101 tttcacgccc tgatgcatca acatcattaa cgcctcaagt acgccgccgc caatcgcccg
    14161 ggctttatca gaaacgctgg tgaaatcagc ggtttcacca cgaggatcga tatcaccaat
    14221 tttaaaacca ccaaccactg ccaggccgtc gttcaacaag ccacgcacca tccccgtcaa
    14281 cggtgcttta atttcatgct caccaatcca ggcaatcaca tcgccctctt tcaccagatc
    14341 gcctaatttc acgttggatc gcataatgcc tgcagcagga gcacggatca cccgtcgggt
    14401 ggtatgcccc ataatattgc caggaacatc ggtattctcc tgcgcacaac cagagtaaat
    14461 cacctgaccg agccagtgcc cgcgatttgt ttcaattacc gcatgacaat ccttccctgc
    14521 agtaaagccc ggcccaagcg cgattgttac tggtgccata tctgcccgcg ttcccaaatt
    14581 ctgtttcgcc agaatagcgt ccaccacgca aagcggtttc agttcatcaa gcaatgaaca
    14641 ggcgggatct accatcacag ggatgaatcc gcgttcggta agtttcatcg cttccgcaga
    14701 gctggttgcc aggcgagcag tgacgccttc gaccgtcatt tcgccatcga acacggcctg
    14761 ggcaaacgcc acggtacaac gaatcactgt cggtttttcc acttccagca tgatcacttt
    14821 aaaacccgca tgatacagac gtagcgccac accactggcg atatctcccg caccgcgaat
    14881 caccaccagc tggtgtccgg aaggatgaga aggtttcatc atcagcccac ccggcgcgtt
    14941 atttttcacc cataatattt ctgccagcac gctgatggcg atctcctgcg gcgtttccgc
    15001 acctatgttg taaccaacgg gcgcatgtaa acgggcaata tgttcttcag ccacgccttt
    15061 ctcacgcaat tgacgcagga aaagctgaac cttgcggcga cttgccagca accccaacca
    15121 tgcaatgggc tgttcaatga gtttgtcgag ggcttcacga tcctgattat tcgtggcaat
    15181 caggacaaaa ttatcagggc gaatatccag ttcttccact gccgcaccaa acgactcagc
    15241 atggagaagc gtggttgatg gtgggaatag ttcaggattg aggctttcgc gataaatatc
    15301 ggcaacggca atatcaaatc ctaatagcgc cgcactctgg gctatcgccc ggttgacatg
    15361 ccccgcgcca atcaacacca gacgtggacg cataccatgg acgctgataa atactgacat
    15421 tgcacctcca caatctgacc cgacagcatc cgcaccgtta cgagccatac gtccatggaa
    15481 taatcgcggc ttacgttcct gcaatgcctg aagtgactct tcaatcacct tccgctcaac
    15541 cattccgcca ccaattgtac cgacgataga accatcggcg cgcactaaca tttgtgcaga
    15601 atggcgggga gtcgagcctc ggctatcaac aatttgcgcc atcgcaaacg gacaattttg
    15661 ctcttcgagt tttgcagcct ctgtgaaaat attcatacca acctcaatgg atatcctttc
    15721 agtaacccgg aatacccggg ccgctaattc acaaatctgc gcgcgattgc aggatgttct
    15781 tgtatatcgc ccagccagat tgcctctacg tcgtgttgtt gcagcggttg aaggagctcg
    15841 ctttgtgcaa tcgcattctc acattgagaa aaacggttaa tgaaccagac tcgccgacaa
    15901 ccttgcggta cgtttttaaa cgccccctga gggtggcgaa ccagcgcaac gagatcgctc
    15961 agttgcaagg ttgcatcagg tgttaacccg gtaatatcag caaactgcga ccagcgatgg
    16021 acattttctg tgctcacttt cgcgcccaaa gtatgtcctc ccatcacagc aatcacgcag
    16081 caactgcttt tgggtatgca aggttcgtgc tcatcaggcg cttttaacgg cattccacgc
    16141 gagccatctg cttcaatgag aattacgtca cactctggtc gttgcaccag tgcatcaatc
    16201 gcttctggcg taaatccctg cacttttccc tggttcgctt tccagctgtg aaaacaaaat
    16261 gaaatggggg atgtaagaga cgcatgagga agcatggcgg gatcacgaca gaaaaccacg
    16321 ggccagtgag acgttggcat aaacatatgt gtcgtcgtcg tgattaacac acgcctgccg
    16381 ctagcctgga gcagttctgc cagccaaaaa agcaagctgg ttttcccccc agcaccaaca
    16441 acagaaatca cagtgggacg tttctgcgca cctaaatcaa tgactaatgc cgatgggtca
    16501 actatacttt tcacgagtct ttatgacctc tctgggataa attatcccca acttaatcca
    16561 tcaggaagta acgcaattat caggcgttat tagcccctat aaataatgga accactatgt
    16621 cagccatcga ctgtataatt accgctgccg gattatcatc aagaatgggg caatggaaaa
    16681 tgatgttccc ctgggaacag ggaacaattc ttgatacaag tatcaaaaat gcgttgcagt
    16741 tttgtagccg aattatttta gtcaccggct atcgtggtaa tgaactgcac gaacgttatg
    16801 cgaaccagag caatatcact attattcaca acccagatta tgcgcagggt ttactaacat
    16861 ccgtcaaggc cgcagtaccc gcagtgcaaa cagaacattg ttttctcacc cacggtgaca
    16921 tgccaaccct caccatcgat atttttagaa aaatctggtc gttacgaaat gatggcgcaa
    16981 tactgccact ccataatggc atccccggcc atccgatttt agtgtcaaaa ccatgcctga
    17041 tgcaggcaat ccagcgaccc aatgtcacca atatgcgtca ggcactcctt atgggagatc
    17101 attattccgt cgaaatagaa aatgcagaaa taattttaga tattgatact ccggacgact
    17161 ttattacagc gcaaaaaagg tatactaaaa tttagaaaat gtagttaaac gattgcgttc
    17221 aaatatttaa tctctccagc actctttatc ataatgataa ggaatcactg attttgagaa
    17281 aagagaaaaa gtgataaatg ggctgacaat aataattttc aggagtgatt ttaattctca
    17341 tttggcacct gtgagttcat tcacaaaaag cctttaagtt tcgagccacc tcacattttt
    17401 ttatatttcc cgccaaacct ggcaagagtg gtgcgattgt tgctctatcc ccctaaacca
    17461 ccggatttct caacaccggt cactcaatga tatctgtata agctaaggag agggttatgg
    17521 gggatattat gcgtcccatt ccgtttgagg aacttttgac gcgcatattt gatgaatacc
    17581 aacaacaacg ctcaatcttt ggtattcccg agcaacagtt ttactcacct gtaaaaggta
    17641 aaactgttag cgtcttcggt gaaacctgtg ccactcccgt cggccctgcc gctggcccgc
    17701 acacgcagct cgcgcaaaat attgtcactt cctggctgac tggcggacgc ttcatcgaac
    17761 taaaaaccgt ccaaattctt gaccgcctgg agctggaaaa gccctgtatt gatgccgaag
    17821 acgaatgctt taacaccgaa tggtctaccg aatttacctt gcttaaagcc tgggatgaat
    17881 acctcaaagc ctggtttgcc ctgcaccttc tcgaagcgat gttacagcct tctgattccg
    17941 gtaaatcgtt catctttaat atgagcatcg gttacaacct cgaaggtatt aagcaaccgc
    18001 cgatgcaaca gttcatcgac aatatgatgg acgcatctga ccatccgaaa ttcgctcaat
    18061 atcgcgatac gctgaataaa ttactccagg atgacgcatt tttagcccgc cacggattgc
    18121 aggaaaaacg cgaaaacttg caagccttac ccgctcgcat ccccaccagt atggtgcagg
    18181 gcgtccccct ctccaccatg cacggctgtc ctccgcatga aatcgaagcc atttgccgct
    18241 acatgctgga agaaaaaggg ctcaacacct ttgtcaaact taacccgacc ttactggggt
    18301 acgcgcgtgt tcgtgagatc ctcgatgtct gcggtttcgg ttacataggc ttaaaagaag
    18361 agtcatttga tcacgacctc aagctgacgc aagcactgga aatgctggag cgcctgatgg
    18421 tactggcaaa agaaaaatca ctcggctttg gcgtaaaact gactaacact ctcggcacca
    18481 tcaacaataa aggcgcactg cctggtgaag agatgtatat gtcaggccgt gcgctgttcc
    18541 cgctctccat caatgttgca gcagttctct ctcgcgcctt tgacggcaaa ctgcccattt
    18601 cttattccgg tggtgccagt cagctgacta tccgcgatat ttttgataca ggtattcgcc
    18661 ctattactat ggcaaccgac ctgctgaaac ctggcggcta tctgcgctta agtgcctgca
    18721 tgcgcgagct ggaaggctcc gacgcctggg gacttgacca tgttgacgtc gaacgactga
    18781 acagactggc agcagacgcg ttaaccatgg aatacaccca gaaacactgg aagccagaag
    18841 agcgtattga agtggcagaa gacctgccgc tgaccgattg ctacgttgcc ccctgtgtta
    18901 ctgcctgcgc tatcaagcaa gatattccgg aatacatcc
//
LOCUS       DAJTVE010000082        18687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000082 DAJTVE010000000
VERSION     DAJTVE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(97..1968)
                     /gene="ppiD"
                     /locus_tag="OO439_002681"
     CDS             complement(97..1968)
                     /gene="ppiD"
                     /locus_tag="OO439_002681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6317011.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2160..2432)
                     /gene="hupB"
                     /locus_tag="OO439_002682"
     CDS             complement(2160..2432)
                     /gene="hupB"
                     /locus_tag="OO439_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6317012.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2641..4995)
                     /gene="lon"
                     /locus_tag="OO439_002683"
     CDS             complement(2641..4995)
                     /gene="lon"
                     /locus_tag="OO439_002683"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6317013.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5183..6457)
                     /gene="clpX"
                     /locus_tag="OO439_002684"
     CDS             complement(5183..6457)
                     /gene="clpX"
                     /locus_tag="OO439_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6317014.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6583..7206)
                     /gene="clpP"
                     /locus_tag="OO439_002685"
     CDS             complement(6583..7206)
                     /gene="clpP"
                     /locus_tag="OO439_002685"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6317015.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7452..8750)
                     /gene="tig"
                     /locus_tag="OO439_002686"
     CDS             complement(7452..8750)
                     /gene="tig"
                     /locus_tag="OO439_002686"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6317016.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9094..9411)
                     /gene="bolA"
                     /locus_tag="OO439_002687"
     CDS             complement(9094..9411)
                     /gene="bolA"
                     /locus_tag="OO439_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6317017.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9716..10294
                     /locus_tag="OO439_002688"
     CDS             9716..10294
                     /locus_tag="OO439_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6317018.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10338..11813
                     /gene="ampG"
                     /locus_tag="OO439_002689"
     CDS             10338..11813
                     /gene="ampG"
                     /locus_tag="OO439_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6317019.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12274..13221
                     /gene="cyoA"
                     /locus_tag="OO439_002690"
     CDS             12274..13221
                     /gene="cyoA"
                     /locus_tag="OO439_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6317020.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13243..15234
                     /gene="cyoB"
                     /locus_tag="OO439_002691"
     CDS             13243..15234
                     /gene="cyoB"
                     /locus_tag="OO439_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6317021.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15224..15838
                     /locus_tag="OO439_002692"
     CDS             15224..15838
                     /locus_tag="OO439_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6317022.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15838..16167
                     /locus_tag="OO439_002693"
     CDS             15838..16167
                     /locus_tag="OO439_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6317023.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16179..17069
                     /gene="cyoE"
                     /locus_tag="OO439_002694"
     CDS             16179..17069
                     /gene="cyoE"
                     /locus_tag="OO439_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6317024.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17254..17565
                     /locus_tag="OO439_002695"
     CDS             17254..17565
                     /locus_tag="OO439_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6317025.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17549..18091
                     /locus_tag="OO439_002696"
     CDS             17549..18091
                     /locus_tag="OO439_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6317026.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18147..>18687)
                     /locus_tag="OO439_002697"
     CDS             complement(18147..>18687)
                     /locus_tag="OO439_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6317027.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4180 a         4915 c         4539 g         5053 t
ORIGIN      
        1 gtaaacaaat ggcaatgttc agaaatggaa aaggccgcga aagcgacctt ttcttgttac
       61 agctgcaacg catttttttg cgaggcggct tcaggattat tgctgttcca gcgcatcgcc
      121 aattttgatt ttcgcctctt tacgcaggtt actcatcaga gcttcaaaga cgatttgtgc
      181 gttgttctgg gtgataccct gcaccatcgc ttttttctga tcttccggca ttgaaccttg
      241 tttcacttca tccagcgcca gcagaaccac attaccttgc atatcggtcg ccataccgta
      301 gctcggtttg tctttcgctg gcagtggcag tgcaaacgcc gcctggctaa tcgggtcacg
      361 accggaacgg cttaaggttt tcggctcgcc aaatttcaga ccggcagcct gcatagcttc
      421 cgcccctttg ccggctttca aatcaaccag cagtttctca gcatccactt tcgcctgttg
      481 ttcagctttg ttgtgctgaa ccagtgcctt aacttgttcc tgaacatctg ccaacggttt
      541 caccgcttcc ggtttgtgct cgctgatgcg cagcacgaat gcgcggtcgc cgtctacggt
      601 gatgatgtca gagttgatgc ccggcgcgcc gttttcacct accagaccgc cgttaaagat
      661 agcgtcggca accggcttga agttcaactc ttccggcagg ttatctttgc tgaaccaacc
      721 cgtctgagtg gctttaacgc cggcagcttg ctctgcaccg gccagagact cggtgtcgtt
      781 gcttgccgca tcgctcactt tctgctgcag cgcatagtac gcatcgaggg ctttttcgtg
      841 tttcactttc gccgcaatgt cgtcacgtac ttcgtctaac gatttcactt tcgctggctg
      901 aatgtcgtcc agacgtacaa tcaggaaacc gaccgaagat ttgatgacac cagacagttg
      961 gcctttttct ttcagaccag catttttcag ttcgtcgggg atagtggcat cttctaacca
     1021 acccatatcg ccgccgttac gggcagagat aatatcggca gatttttctt tggctaatgc
     1081 agcaaaatca ccgcctttat tcagctcatc aagtaccgct ttcgcttcat cttcagtttt
     1141 ggtctggatg atgctgtagc gggtacgctg cggctgggtg aattgatcct gatgctgatc
     1201 gtagtagctc tggatatccg catcgctaac cggttgctgc atcgttgcgg catccagctt
     1261 gatgtaactc acgcggaatt gttccggcgt catgaaattg tttttgtttt gttcgtagta
     1321 gctggcaatc tcctgttcgg tcacaggctg cttcgccgcc agcgcgttaa catcgatagt
     1381 cgcctcacgc accacgcgtt gttgcgcgac cagtgccgcc agctcgtcgg tttcaccttt
     1441 cagcataaaa tcggtaccgg caacaccgtt aatcagctgt tgggtagtga gctggttacg
     1501 cagcgcctgg gcgtactgat cggcggtcat ccccatctgg ttgaggatac cgttatagcg
     1561 gctgttatca aatttgccgt caacctggaa ggctggggtc gcgaaaatcg cctgtttaac
     1621 ctgctcatca ctgataccca gtttcagctc acgagcgtac tgatccaaca gcgcctcgtc
     1681 gatcagacga ttcagcacct gttgacgcag ggttttcata tagccttcgt tcgctgccag
     1741 ctcggagtat tgatcgccca gctgttgctg catgcgatta cgctcgctgt taaaggcgtt
     1801 ctcgaactgc ccacggctga tttcctggtc attcactttt gcggcgtagt tattgcctcc
     1861 gccaatcagg taaccactca cgccggtcaa tatgaacgac acgataatga taccgaaaat
     1921 aatcttgagc acgagactgt ttgcagccgt gcgtaagctg tccatcatgg tgtaacaaca
     1981 ctccgctgta gatgactgtg tacctcacgc aacgtatcga tgcgcgcaaa ggcctattgt
     2041 gacaagaaac gggggcaatt gtcagcccac aagacacaga aagtcaccga atacaaataa
     2101 aaaaggcaca tcagtagatg cgcccttgaa cttcgtcaca tccccactgg ggacaacgct
     2161 tagtttaccg cgtctttcag tgctttacct gcacggaagc tcggtacttt agcggcagcg
     2221 atggtgatct ctttaccggt ctgcgggttg cggccagtac gggcagcacg ctctttaacg
     2281 gcaaaagtac caaaacctac cagtgctaca tcatcccctt ctttcagaga ttcagttacg
     2341 gaagcaataa tagcatctaa cgcacggcca gccgcagctt tagagatatc agcccctgca
     2401 gcaatcttgt cgatcaattg agatttattc actcttctct tcctctttat aatttatatc
     2461 gcacttgaat ccttcaaggt acgaacgcgc agcagttata tcaggccagc catcccctta
     2521 caacacccct taataagggc aagcccgatc cgccatctaa cttagcgaga caaaaaaagg
     2581 ctggcaagcc cgaattagcc tgccagccct gtttttatta gtgcattttg cgcgaggtca
     2641 ctattttgca gtcacaacct gcatgccaga cggttcattt tgcagcgcca gagtcagaac
     2701 ttcctcaatg cgcttcacag gatgaatgtc cagatcggca attacgttgt caggaatctc
     2761 ttccagatcg cgtttatttt cgaacggaat tagcactgtt ttaatcccgc cgcgatgcgc
     2821 tgccaggagt ttttctttca aaccaccgat cggcagtacc tgaccacgca gagtgatctc
     2881 accggtcatt gccacatcgg cacgaaccgg gttaccggtc aggcaagaaa ccagcgcggt
     2941 gcacatagca ataccggcac tcggaccatc tttcggcgtc gcaccttccg gtacgtggac
     3001 gtggatgtca cgtttttcgt aaaaatcagg gttgatcccc agtttttccg cacgcgcacg
     3061 aaccaccgtt aacgccgcct gaatagactc ctgcatcact tcgccgagcg aaccggtata
     3121 ggtcagtttg cctttacccg gaacacatgc ggtttcaatg gtcagcaagt caccgcccac
     3181 ttccgtccac gccagaccgg ttacctgacc gacacggttt tcgttatcag cgcgaccata
     3241 gtcgaaacgc tgaacaccga ggtagtcatg caggttatcg ccgttaattt cgatatgttt
     3301 taatgactta tcgagcagta actgcttaac cgctttgcgg cacagtttgg agatttcacg
     3361 ctccagacca cgcacgcccg cctcacgggt gtagtaacga ataatgccga taatggcgct
     3421 atcgtcgacg gtcagctcac cttttttcag tgcattacgt tcaatctgct tcggcagcag
     3481 gtgacgtttg gcgatgttca gtttttcatc ttcggtataa ccggagaggc gaatcacttc
     3541 catacgatcc agcagcggtg ccggaatgtt catggagttc gacgtcgcga caaacatcac
     3601 gtcgctgaga tcgtaatcca cttccaggta gtggtcgctg aacgctacgt tctgctctgg
     3661 atccagcact tcaagcagtg cagaggccgg atcgcctcgc atgtcagaag acattttgtc
     3721 gatctcatcg agcaggaaca gcgggttttt cacgcccact ttcgccattt tctggatcag
     3781 tttacccggc atagaaccga tgtaagtacg gcggtgacca cggatttccg cttcatcacg
     3841 cacgccgccc agcgccatac ggacatattt acgcccggtg gctttggcaa tggactgacc
     3901 aagagaggtt ttacctaccc ccggcggccc taccaggcag aggatcggtc ccttgatttt
     3961 gttgacacgg ctttgaaccg caaggtactc aaggatgcga tctttcacgc gctccagacc
     4021 ataatggtcg gtatcaagga tttcctgcgc ctgacgcagg tcttttttga ccttgctgcg
     4081 cgcattccac ggtacctgta ccatccagtc gatataacca cgcactacgg tcgcttctgc
     4141 cgacatcgga gacatcattt tcagcttctg caactctgct tccgctttct cttttgcctc
     4201 tttcggcatc ttcgccgcgt cgattttgcg cttcagggct tcgttttcgt ccggcgcgtc
     4261 gtccatttca ccgagttctt tctgaatagc tttcatttgc tcgttcagat agtactcacg
     4321 ctgggatttc tccatctgct ttttaacgcg gttgcgaatg cgtttctcaa cctgcagcag
     4381 atcgatttcc gattccatca ttgccatcag atattccaga cgttcgttaa cgtcggacat
     4441 ctccagaacg gactgtttgt cagccagttt cagcggcata tgtgcagcaa tggtatccgc
     4501 cagacgcgcc ggatcgtcga tgctattcag cgacgtcagc acttctggtg ggattttttt
     4561 gttcagcttg atgtagcctt cgaactggct gattgcagta cgcaccagca cttcctgttc
     4621 ccgctcatca atggtcggcg actccagata ctccgccttc gcagaaaagt gttcgccatt
     4681 gtcagagagc gcagaaatac gcgcgcgctg taacccctcg accagcactt tgacggtgcc
     4741 gtcaggcagt ttcagcatct gcaatataga ggccacggtc ccgacggtga aaagatcgtt
     4801 tacacccggc tcatccgttg aagcttcttt ctgcgcgacc agcataattt ttttatcatg
     4861 gtccatcgcc gcttccagac aacggataga tttttcccgc ccgacaaata aggggatgac
     4921 catgtgcgga taaaccacca catcgcgcag cggcaatacg gggatttcaa tgcgttcaga
     4981 acgctcagga ttcatagagc tctctcttag tttaatttcc gccaggtaat cagatgacac
     5041 gactgtgctt cacgccatct attaacatgt acgtcagtat atggggatgt ttcccccaca
     5101 ttcaacgccg agaatagagg aaaaattaaa ggggagataa aatcccccct ttttggttaa
     5161 ctaattgtat gggaatggtt aattattcac cagatgcctg ttgcgcttcc ggcttaccat
     5221 aaatcagcaa cggtttgctt tgaccatcaa ttaccgactc gtcgataacc actttttcga
     5281 cgtcttccat ggacggcaga tcgtacatgg tatcgagcag tgcggcttct acgatggaac
     5341 gcaggccacg ggcaccggtt ttacgcgcca tcgctttctt agcgatagca tccagcgcct
     5401 cgtcacggaa ttccagatcc acgccttcca gattaaacag cgcctgatac tgcttggtca
     5461 gggcgttttt cggctctttg aggatctgaa tcagagcttc ttcgctcagt tcattcaacg
     5521 ttgctacaac cggcagacga ccaatgaact cagggataag accaaacttg atcagatctt
     5581 ccggttcaac ctgcgccagc agctcgcctt cgcttgcttt gtcggacttc gcttttaccg
     5641 tcgcgccaaa accaatgccg gagccggttt ctacacggtg ggaaatcact ttatccagac
     5701 cggcaaacgc accgccacaa ataaacagga tcttagaggt atcaacctgc aagaattcct
     5761 gctgcggatg tttacgccca ccttgcggtg gaacagcagc taccgtacct tcgatcagtt
     5821 tcaacagtgc ctgctgtacg ccttcaccgg aaacgtctcg ggtaatggac gggttgtctg
     5881 acttacgaga aatcttgtcg atttcatcga tgtagacaat cccgcgctgc gctttctgta
     5941 cgtcgtaatc gcacttttgc aacagcttct gaatgatgtt ttcaacgtct tcgcccacat
     6001 aaccggcttc ggtcagcgtg gttgcgtcgg ccatggtgaa cgggacgtcc agcaggcgcg
     6061 ccagcgtttc agccagcagc gttttaccgg aaccggtcgg accgatcagc agaatgttac
     6121 ttttgcccaa ctcgacacca ttgctggtat cgccgttgcg cagacgtttg taatggttgt
     6181 ataccgcgac cgccagcact tttttcgcct gttcctggcc gataacgtaa tcgtccaggt
     6241 ggttgcgaat ttcatgcggc gtcggtagcg cactgcgttc acgatgcggt gcaacttctt
     6301 taatctcttc gcgaatgatg tcgttacata aatcaacaca ttcgtcgcag atatacacgg
     6361 atggaccggc aatcagcttg cgcacttcat gctggctttt gccgcaaaaa gagcaataca
     6421 gcaatttgcc tgagccatct ttgcgtttat ctgtcatggg tcaaaacctc ttctttgttc
     6481 tttgtgccgc acacgacgac gcaaatgcca ttctcaggcg caagccgctt acagcgttgt
     6541 gccgccctgg ataagtatag cggcacagtt gcgcctctgg catcaattac gatgggtcag
     6601 aatcgaatcg accagaccgt attccaccgc ttcaggggcg gaaaggaagc gatcgcgctc
     6661 ggtatcacgt tcaatctgtt ctaatgattg acccgtatga agcgccataa gttcattcat
     6721 gcgcccttta actttcagaa tttcacgggc atgaatttcg atatcggtcg cctggccctg
     6781 gtagccgccc aacggttggt gaatcatcac gcgcgaattc ggcaggcaaa aacgtttacc
     6841 ttttgcccct gcggtcagca agaaagcgcc catcgaggcc gcctggccca tacagatggt
     6901 gctgacatca ggcttgataa actgcatggt gtcatagata gacatcccgg cagtgatcac
     6961 cccgcctggg gagttaatgt acagatagat atctttttct gggttttccg cttctaggaa
     7021 cagcatctgc gccacaatca ggttcgccat gtggtcttca acctggccag tcagaaaaat
     7081 gacgcgttcc ttaagtagac gagaatagat atcaaaagag cgctcaccgc gtgaggtctg
     7141 ttcaatgacc atcggcacca gcgccatatg gggtgcaaag ttatctcgtt cgccgctgta
     7201 tgacatttcc gtctcctgga taaaattgaa aaaacctgct gtaccgattg taaccttatg
     7261 gacggattat cagctagtcc ctgtgttatg ggtacggcat caccatattt caagcataac
     7321 aatcttttgt tattacgcta acaccgaaag aggcttttcg cacggttcca tgcaaaattc
     7381 atgacaaaaa aaagcccacc acctggcggt gacgggcctt tgtgcgaatt tagcgcgtta
     7441 tgctgcgtaa attacgcctg ctggttcatc agctcgttga aagtggtttc tttttcagtc
     7501 actttcgctt tcgccagtac agcttcaaca gcctgttctt ccagagcaac attgcgcatg
     7561 ttgtccatca gttctttgtt tttgctgtag aactcgataa cttctttcgg atcttcgtac
     7621 gcagaagcca tctcttcgat caggcctttc acgcgctctt cgtcagcttt cagttcgttg
     7681 gtgcggataa cttcgcccag cagcaggcca acaactacgc ggcgtttagc ctgttcttcg
     7741 aacagttcgc gcggcagttc cagagcttgt ttttcgttgc caccgaaacg ctgtgcagcc
     7801 tggcgacgca gaacgtcgat ttcgctgtcg atcagcgcag ccggtacgtc gatgtcgtta
     7861 gcttttacca gaccttcgat cgcctgagac ttaacgcggt tacggatggc gctcttcagc
     7921 tcgcgctcca tgtttttacg cacttcagcg cgcagacctt ctacggaacc atcttcaacg
     7981 ccgaaacgtt tgatgaattc tgcagtcagt tccggcagtt cacgctcttc aactttcttc
     8041 aggttgatag cgaatttcgc tgctttacct ttcagatttt ctgcgtggta ttcttccggg
     8101 aaggtcacgt cgatggtgaa ctcttcgcca gctttgtggc ctttgatacc gtcttcaaag
     8161 cccgggatca tacgaccctg gcccatcgcc agtacgaaat cagacgcttt accgccttcg
     8221 aactcttcgc cgtctacaga accggtgaag tcgatggtta cgcggtcttc tgcttcaacg
     8281 gcgccgtctt tttctttcca ggtcgcctgc tgtttacgca gagtatccag catgccgtca
     8341 acgtcagcgt cggtcacttc aacgatcggt ttttcaactt cgatcgcttc cagaccctgc
     8401 agttcaactt ccggataaac ttcaaactct acagagtagg tgaagtcttc acccagcttg
     8461 tattcgcccg gaacataagt cggtgcgcca gccggattga ttttttcttt aatgatggcg
     8521 tcaatgaagt tacggctcat caggtcaccc agaacgtcct ggcgtacaga cgcgccataa
     8581 cgctgagcaa cgatattcat tggcactttg cctttgcgga agccgtcaat acgtactttt
     8641 ttcgcaacgt tgaccagctc gcttttaaca gcggtctcga tgctgtcagc agcgatagta
     8701 atcgttacac ggcggccaag gccttgagtg gtttcaactg aaacttgcat cttgttacct
     8761 caaaaaatca cagtgctcgg tcaactctta ccccgcaagc acaggttgtt ggcttcgcgg
     8821 aaccgggatg ttcttataat caatcacatt ccctgttacc agaatcatcc cgaaagcatt
     8881 caaataggac gcggcattat agcggcatca cttttatgag tcgagaacgg ttattgcgcg
     8941 ctgtcgcgta ttttttcaca attcccgggc aattttcgtc tgaatgctgg gcaaaaagcg
     9001 caaaatccag acaaaacaaa acggcccgca ggccgttttt cataaaatct ttagcaacat
     9061 actggaaaag cgccgacagt tgcaaatgcg tttttacgcg atgcttcctg ctccacgaca
     9121 gggaggagag gcaaagacgg tgtcctgcaa cccttcccac tccttaatag tgtaagtatg
     9181 cagagccagc gcatgaacgg tagtagagag ttcctccgct aaagtactgt aaatcattcg
     9241 atgacgattc agaaaacgtt cacccgtaaa acgatcgctg accagcacaa ctttaaaatg
     9301 gctttcagag ccggctggga cattgtgacg atagctttca tccactactt cgaggaatac
     9361 gggttggaac gccgccctta atttttcttc tatccgctca cgtatcatca tgaaatatct
     9421 cctccgacaa cgctgagatg tcatccatcc ctttaaatac tagccgcttt taccgtttcc
     9481 attgcagctt aacaacaaat atttaggttt cgtcctggtt tttccgtcaa gcgtatgaag
     9541 tttactgatt taaagattga aacacaggcc tcgcatcagc gtttctttta ccaggggtga
     9601 ttatttcgcc aacatgatga atttacatgc cttacccact tcccctcgcc gttggcaatg
     9661 ttatgatggc gggaattttt accaaacacg cttaagagtc tctgagagcc cgaacatgtt
     9721 caaaaaaatc ctcttcccgt tagttgctct gtttatgctt gcaggatgcg caaaaccgcc
     9781 aacaactatt gaagtttccc cgacgattac actgccacag caggatccaa gcctgatggg
     9841 cgtcaccgta agcattaatg gtgccgatca gcgtaccgat caggcgctgg caaaagtcac
     9901 ccgcgataat caaatcgtta ccctgaccgc ctcccgagat ctgcgtttcc tgctgcaaga
     9961 agtgctggaa aaacagatga ccgcgcgtgg ttacatggtt ggtccgaatg gcccggttaa
    10021 tctgcaaatc atcgttagcc aactgtatgc tgacgtgtcc cagggcaacg tgcgctacaa
    10081 catcgcgacc aaagcggata tcgccatcat cgctaccgcg cagaatggca acaaaatgac
    10141 caaaaactat cgtgccagct acaacgttga aggtgcgttc caggcctcca acaaaaatat
    10201 cgccgatgcg gttaacagtg tgctgaccga taccatcgct gatatgtctc aggacaccag
    10261 catccacgaa ttcatcaagc agaacgcgcg ttaatttctg ccctctggcc cggtgcaaac
    10321 cgggcctgta gacgcccatg tccagtcaat atttacgtat ttttcaacag ccgcgttcag
    10381 ccatattgct gatcctgggt tttgcttccg ggctaccgct cgccctgaca tccggtacct
    10441 tacaggcctg gatgacggtc gagaatattg atctcaaaac cattggtttc ttctctctgg
    10501 taggccaggc ttacgttttt aaattcctct ggtcaccgct gatggaccgc tacacgcccc
    10561 cattttttgg gcggcggcgc ggttggctgc tcgccacgca aatcctgtta ttagtcgcca
    10621 ttgcggcgat gggttttctc gaacctggca cccaactccg ctggatggcg gcactggcgg
    10681 tggtgatcgc tttttgctcg gcctcccagg atattgtctt cgatgcgtgg aaaaccgatg
    10741 tgctcccggc agaagaacgt ggtgcgggcg cggcaatcag cgtgctgggt taccgtttag
    10801 ggatgctggt ttccggcggc ttagccctgt ggctggcaga taaatggctg ggctggcagg
    10861 gcatgtactg gttgatggcg gcgctgttga tcccttgtat tatcgcgacg ttgcttgcac
    10921 cagaaccgac cgacaccatt cctgtgccaa aaacgctgga acaagcggtt gttgcacctc
    10981 tgcgagattt ctttggtcgc aataatgcct ggcttatttt gcttcttatc gtgctgtata
    11041 agctgggcga cgcattcgcc atgagcctga caaccacgtt tttgattcgc ggcgtcgggt
    11101 ttgatgcggg tgaagtaggc gtggttaaca aaacgcttgg cttactggcg accattgtgg
    11161 gcgcattgta cggtgggatt ttgatgcagc gcctgtcgct gttccgggcg ctgctgattt
    11221 tcggcatttt acaaggtgcg tctaacgctg gttactggct gctgtcgatt actgataagc
    11281 atctctacag catgggcgca gccgtctttt tcgaaaacct ctgtggcggg atgggcacat
    11341 cagcctttgt cgcactgtta atgacgctgt gtaataagtc attttccgct actcaatttg
    11401 ccctgctctc agcgctttcc gctgtaggac gagtttatgt cggccccgtg gcgggttggt
    11461 ttgttgaagc acacggctgg tcgacattct atctattctc cgtcgccgct gccgtaccgg
    11521 ggcttatttt gctgctggtt tgccgccaga cgcttgaata tacacgagta aatgacaact
    11581 ttatctcccg taccgaatat ccgacaggtt atgcctttgc catgtggaca ctggcggcgg
    11641 gcgtcagcct gttggccgtg tggttactgc tgttgacgat ggacgcgctg gatttgacgc
    11701 acttctcttt cctgcctgct ctgctggaag tcggggtttt agtcgccctt tctggcgtcg
    11761 tgcttggtgg tttgctggat tatctggctc tacgaaaaac gcatctgacg taatctgtaa
    11821 atattattta gctgtagtaa tcactcgccg taattattac ggctaaataa acatcagtat
    11881 cgcttaataa tatttgttct tttgtgcggc ttagcgtttg gaaattattg acgcaattta
    11941 tgaattctat tttttcttac acgattcagc taatgagtct ttattttccc cccacccaat
    12001 tgtcaccata atgataatta tttgttaaac aattgtttta ttcaagattg gttataccaa
    12061 ttgcccgtcc aaactccttc tgcactcccc tttcgttata acgccctttt gcaacagctt
    12121 cttaaaatca acttgatatg ttttgcaaca tatgtaacct gacagccaaa tccaagtaac
    12181 aggaatttaa tcatgtttac agtaatgtaa cctccccgta aaatgcccac acactttaaa
    12241 cgccaccaga tcccgtggaa ttgaggtcgt taaatgagac tcaggaaata caataaaagt
    12301 ttgggatggt tgtcattatt tgcaggcact gtattgctca gtggctgtaa ttctgcgctg
    12361 ttagatccca aaggacagat tggtctggag caacgttcac tgatactgac ggcatttggc
    12421 ctgatgttga ttgtcgttat tcccgcaatc ttgatggctg ttggtttcgc ctggaagtat
    12481 cgtgcgagca ataaagatgc taagtacagc ccgaactggt cacactccaa taaagtggaa
    12541 gctgtggtct ggacggtacc tatcttaatc atcatcttcc ttgcggtact gacctggaaa
    12601 accactcacg ctcttgagcc tagcaagccg ctggcacacg acgagaagcc cattaccatc
    12661 gaagtggttt ccatggactg gaaatggttc ttcatctacc cggaacaggg cattgcaacc
    12721 gtgaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac ctccaactcc
    12781 gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat ggccggtatg
    12841 cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat ctccgccagc
    12901 tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc
    12961 gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga acaccatgtc tgacatggct
    13021 gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt ctccaacgtg
    13081 aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa gagcatggac
    13141 atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat ggacatgagc
    13201 cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg aaaattatca
    13261 cttgatgcag tcccgttcca tgaacctatc gtcatggtta cgatcgctgg cattattttg
    13321 ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta cctgtggaaa
    13381 gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt
    13441 gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc
    13501 tcggcgggcg aagcgggctt cctgccacct caccactacg atcagatctt caccgcgcac
    13561 ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg
    13621 gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc
    13681 tggtttactg ttgttggtgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg
    13741 cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt
    13801 gtcgattact ggatatggag tctccagcta tccggtatag gtacgacgct taccggtatc
    13861 aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt caagatgcca
    13921 gtatttacct gggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg
    13981 acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac
    14041 gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg ccacccggaa
    14101 gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg
    14161 cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg
    14221 tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc
    14281 ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat cttcaactgg
    14341 ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gaccatcggt
    14401 tttatcgtca ctttctcggt gggcgggatg actggcgtgc tgctggcagt accgggcgcg
    14461 gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt tatcatcggc
    14521 ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc
    14581 aaactgaatg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt
    14641 gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt gagccagcag
    14701 attgacccgc agttccacac catgctgatg attgcagcta gcggtgcagt actgattgcg
    14761 ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg cgaccagaac
    14821 cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg
    14881 cctccgttct ataactttgc cgttgtgccg cacgttcacg agcgtgatgc attctgggaa
    14941 atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat tcatatgccg
    15001 aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg
    15061 atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc
    15121 gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg
    15181 gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt
    15241 gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat
    15301 cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac
    15361 ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact
    15421 gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc
    15481 ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg
    15541 gttgtttggt gccggattta tcgggatgga aatctacgaa ttccatcacc tgattgttaa
    15601 cggcatgggt ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca
    15661 cggtctgcac gtcacttccg gtcttatctg gatggcggtg ctgatggtac aaatcgctcg
    15721 tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt
    15781 cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg
    15841 agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc
    15901 tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac aggagctgcc
    15961 tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat
    16021 ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt
    16081 gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac
    16141 ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc
    16201 aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc
    16261 tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac
    16321 tggttgtggc gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga
    16381 tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg
    16441 tgtacgccac cttgctaggc tttgctggct ttatgctgct gtggtttggc gcgaatccgc
    16501 tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtttat agcctgtaca
    16561 tgaaacgcca ctcggtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg
    16621 tgattggcta ctgtgcagta accggcgagt tcgatagcgg cgcagcgatc ctgctggcta
    16681 tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt
    16741 atcaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc
    16801 acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt
    16861 acgctgggta taaatatctg gtggtcgccg cggcggtgag cgtctggtgg ttaggtatgg
    16921 ccctgcgcgg ttataaagtt gctgatgaca ggatctgggc gcgcaagctg ttcggcttct
    16981 ctatcatcgc catcacagcc ctctcggtga tgatgtccgt tgattttatg gtgccggact
    17041 cgcatacgct gctggctgct gtttggtaac acaacctctc tattaaaaag gtgctacggc
    17101 acctttttta ttgtctgaga tcagagatac ggctaataac cgtatgtctt gacagagtga
    17161 gcagatgaac ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc
    17221 tacgatttca ttcagttaat caaagaggtc ataatgcctg gaaaaacagc cactctaccc
    17281 gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa
    17341 aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc
    17401 gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat
    17461 caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc
    17521 aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc
    17581 catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga
    17641 ttgcggtaac cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa
    17701 tagcgactgt gcggctaaag cacttattga cagacaaagt ggtgaactga tcggcatctg
    17761 tacttttacg gcatattcgc tggaaaaaca acgcgtttct ggcgtccttc agggttcaca
    17821 accttcagaa attggtgttg tcagattagt catgttgggg gtagcacgga agtatcaaaa
    17881 gcggggcttt ggtcaggacc tactatgtga tttttttgaa catgtaaaaa taattcacca
    17941 ggcattacca attaaagggg tttatcttga tgctgaccct gccgccatta atttttatgc
    18001 tcgtctcggc tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt
    18061 tttggcgatt cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt
    18121 ttgccttagc cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc
    18181 aactccacat ccttgccgtt aatggttgcc gttgcggcaa cgattgtggt ccttacagtt
    18241 gcacaagatt ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat
    18301 gaaccaaata ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag
    18361 ccaaccgttt ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga
    18421 ttggccactt cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct
    18481 ttatctaaaa aattatctcg catcccagaa cgggtaaccg taatcacggg taacgtatcg
    18541 tcaaccgttt ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca
    18601 ccataaatgt ggacaccttt atcaagatat tccttcgctt tcttacaatc tacctcaaca
    18661 tactgaccat tggcgtacat aatgccc
//
LOCUS       DAJTVE010000083        18513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000083 DAJTVE010000000
VERSION     DAJTVE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1700
                     /locus_tag="OO439_002698"
     CDS             <1..1700
                     /locus_tag="OO439_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6317028.1"
                     /translation="TLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTKLSFV
                     DNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPWGGVI
                     NTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGD
                     AFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNL
                     PCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLLK
                     KAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQSTIE
                     RALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGDELSP
                     QNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDALYLDE
                     ITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNEMGEL
                     AYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            1701..3350
                     /locus_tag="OO439_002699"
     CDS             1701..3350
                     /locus_tag="OO439_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6317029.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3355..4131
                     /locus_tag="OO439_002700"
     CDS             3355..4131
                     /locus_tag="OO439_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6317030.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4205..5389)
                     /gene="atoB"
                     /locus_tag="OO439_002701"
     CDS             complement(4205..5389)
                     /gene="atoB"
                     /locus_tag="OO439_002701"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6317031.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5420..6742)
                     /locus_tag="OO439_002702"
     CDS             complement(5420..6742)
                     /locus_tag="OO439_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6317032.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6739..7389)
                     /gene="atoA"
                     /locus_tag="OO439_002703"
     CDS             complement(6739..7389)
                     /gene="atoA"
                     /locus_tag="OO439_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6317033.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7389..8051)
                     /gene="atoD"
                     /locus_tag="OO439_002704"
     CDS             complement(7389..8051)
                     /gene="atoD"
                     /locus_tag="OO439_002704"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6317034.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8247..9632)
                     /gene="atoC"
                     /locus_tag="OO439_002705"
     CDS             complement(8247..9632)
                     /gene="atoC"
                     /locus_tag="OO439_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6317035.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9629..11455)
                     /gene="atoS"
                     /locus_tag="OO439_002706"
     CDS             complement(9629..11455)
                     /gene="atoS"
                     /locus_tag="OO439_002706"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6317036.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11622..14471
                     /gene="rcsC"
                     /locus_tag="OO439_002707"
     CDS             11622..14471
                     /gene="rcsC"
                     /locus_tag="OO439_002707"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6317037.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14671..15321)
                     /gene="rcsB"
                     /locus_tag="OO439_002708"
     CDS             complement(14671..15321)
                     /gene="rcsB"
                     /locus_tag="OO439_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6317038.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15338..18010)
                     /gene="rcsD"
                     /locus_tag="OO439_002709"
     CDS             complement(15338..18010)
                     /gene="rcsD"
                     /locus_tag="OO439_002709"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6317039.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4524 a         4532 c         4863 g         4594 t
ORIGIN      
        1 agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac attcagcaat
       61 ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc attagcacaa
      121 aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc atgctcggcg
      181 gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta caaagtagcg
      241 aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga ccatggggtg
      301 gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg
      361 atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac cgtctgcggc
      421 tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat ggcaatggtg
      481 acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag gcgctcggcg
      541 taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag caggcggata
      601 acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg aatctgccgt
      661 gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact aaagatgaag
      721 atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat acaccggaat
      781 ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt
      841 tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg gcaaacagca
      901 atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct acccagacgg
      961 ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg ctggccatga
     1021 actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg cctgcgggcg
     1081 catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt ggtcagggtg
     1141 tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc
     1201 gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa cgccagttgt
     1261 atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg accagcaatg
     1321 agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag caggatgcag
     1381 ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt gagcgcacaa
     1441 catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa ttgctggaaa
     1501 aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa
     1561 cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg
     1621 ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg agtgaatgga
     1681 ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg gcgttggtta
     1741 ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt gcgcagcacg
     1801 atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgactcgc
     1861 tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg gaggataccc
     1921 atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc
     1981 aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg ctctattttg
     2041 cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa cagcgtcagg
     2101 cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta acggttaaat
     2161 cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa gtcttactgg
     2221 atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt cgggtacggg
     2281 tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt ggttttgctg
     2341 gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc aaaacggtac
     2401 taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag gttgccagtg
     2461 ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc acagcggaaa
     2521 agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg acgtttacta
     2581 atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc gaaaagggca
     2641 aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg
     2701 aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata cgtacttttt
     2761 tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc agcgccacgc
     2821 agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct tggacgcagg
     2881 atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc gaagggacac
     2941 tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac cagatactcg
     3001 cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca acctgccagt
     3061 tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac
     3121 aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt
     3181 tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg
     3241 atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat cccaccgggc
     3301 ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga
     3361 aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag tgaaggtgtg
     3421 caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg cgaagatgcc
     3481 agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat
     3541 atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag
     3601 ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga tggttcattt
     3661 gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccggatggg
     3721 caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat tcgtgcacgt
     3781 ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca tgttgtcacg
     3841 cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg tgcactggat
     3901 atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc agttcacggg
     3961 cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt gacgaccgcc
     4021 caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac atttattgta
     4081 ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg attaatgggg
     4141 ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg ggtgttttta
     4201 ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca atgcacagtg
     4261 ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg accagaatac
     4321 gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg acattcactt
     4381 tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca aatgcttcat
     4441 tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac gctttttgcg
     4501 tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg gcataacttt
     4561 taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc ataatcacca
     4621 gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt cctgctttat
     4681 cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc gggaattcgt
     4741 cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg atttcggctg
     4801 taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc gccagttcat
     4861 cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta atccccatat
     4921 gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa acctgtccgt
     4981 ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct aaactcatat
     5041 tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc tgggcggcaa
     5101 gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg cacaccgttt
     5161 ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac ccggcttgca
     5221 acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca cgttcaatgg
     5281 cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt gaaccgttaa
     5341 aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt ttatattcct
     5401 tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg acgccggaga
     5461 agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg gcgattgcca
     5521 gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg taggcgatcg
     5581 ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc acgaaaggtc
     5641 cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt gcagaactaa
     5701 aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat tcggtaatga
     5761 gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag gggaattgca
     5821 ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa gccattggcg
     5881 ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg atggtgatgt
     5941 tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg ccgatgatca
     6001 acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc ggtagctgct
     6061 tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg tcagacggtt
     6121 ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca gtgatgaaaa
     6181 tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata tgctcaaccg
     6241 ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca ccccaggtga
     6301 gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg actcgccggg
     6361 cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg atgacacaag
     6421 cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt gcggcggagg
     6481 cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga ccggtaacga
     6541 tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca ccccacattt
     6601 tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat gttagcaatg
     6661 tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa cgcgtcataa
     6721 aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg cggcgacttc
     6781 aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg caatttcggt
     6841 gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca gcatatgcac
     6901 cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg aaccatcttt
     6961 ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat ccatcgcgcc
     7021 acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg cttcttcgtc
     7081 tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta gcgcaaatga
     7141 catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc cgccagcgtt
     7201 caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt tttccgattg
     7261 cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta aaccgatccc
     7321 taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa tacgttgttt
     7381 cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc accaggggtg
     7441 acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc taccagcgtg
     7501 atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg ataggtcagg
     7561 ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg cagtgggcgt
     7621 tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc tacgacggtg
     7681 ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg aatttgctcg
     7741 attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat gcgccgacct
     7801 gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc attgacgatg
     7861 agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt caggtcgcga
     7921 acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc cataaatccg
     7981 cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa tgtcatcaat
     8041 tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca ggcaatagca
     8101 aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa tatgaaggga
     8161 taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg cgcactgtgc
     8221 aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc gtattcctgg
     8281 agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg ggttcggttt
     8341 ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt aatttcctct
     8401 tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca gactggctga
     8461 cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat cacgacggcg
     8521 cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc ggtaagcagt
     8581 gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact gaatttttgc
     8641 aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg cggcagtatt
     8701 aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc ttttaccatt
     8761 gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat ggtctgatgg
     8821 ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc ttgtagtacc
     8881 agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg ttcaaataat
     8941 ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa cagttcactt
     9001 tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc ctttgcccgc
     9061 cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct ttcaccgcta
     9121 atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat gtccatcatc
     9181 gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc ctggtgcaga
     9241 tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat taaattcaac
     9301 tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag cgcttcgacg
     9361 gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt ctcatggctg
     9421 cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc catcaacacc
     9481 acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt cgcacaatgt
     9541 gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt atcttcatca
     9601 tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc gggttgatcg
     9661 gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga atatcaccct
     9721 gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct gaggctttgg
     9781 tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca cagccgttgt
     9841 cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg cgaattttcc
     9901 ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc tgtttgagca
     9961 gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg aagtcgaccc
    10021 gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat gcattgaggc
    10081 tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc tgaataactt
    10141 tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt attgggtcac
    10201 tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga ttacgtactt
    10261 catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct tgcgccatgc
    10321 ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca cctatcattt
    10381 caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg cggtcacgac
    10441 ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta tccagtactg
    10501 gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc agttcatggc
    10561 gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca ccctggcggt
    10621 caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt gtccgcgttt
    10681 cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt tcaccgggca
    10741 attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg gtgatgatat
    10801 caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt atcagcaaat
    10861 cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg cgaatatctt
    10921 cggttaattc attggcccag atatagccga ggatttcacc attacgctca atggggagca
    10981 ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg gtatttgtac
    11041 gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta ttctgatata
    11101 gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa ccagcaccta
    11161 tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca tttaatgcgc
    11221 ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct agtgcctgat
    11281 taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact gctgaacgtc
    11341 cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc aggattgcca
    11401 tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa tgcatgctga
    11461 aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat gatttctcgg
    11521 cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta ccgctacctt
    11581 aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt
    11641 ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta gtgctctggc
    11701 tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag cgagaatcgg
    11761 aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt caacgcacct
    11821 ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg
    11881 tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg
    11941 ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag tcattggcgt
    12001 ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac cgggtatttt
    12061 taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg ccagtggaac
    12121 gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat gcaccacaag
    12181 atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc gtcgggtatt
    12241 tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg ggtgtcgagc
    12301 agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg gttaccattc
    12361 ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa attaagggcg
    12421 atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc cgggagctgg
    12481 tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg ccggttgata
    12541 aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg
    12601 gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt ccggcggaaa
    12661 gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc gcctccgcgc
    12721 cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt aacgaactgg
    12781 cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg caaattatct
    12841 gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat ctgcaaatca
    12901 gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt
    12961 cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg gaacaggcga
    13021 gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg
    13081 gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcggc
    13141 tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc gatattctcg
    13201 atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg
    13261 aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag cagttaggct
    13321 tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg atgcgtttac
    13381 agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc ggctgtatag
    13441 ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat accggcgtgg
    13501 ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg
    13561 tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca
    13621 tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag tttaccgtgc
    13681 gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta
    13741 aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa accagtttgc
    13801 agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc gaagatgtgt
    13861 taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg accttctgtc
    13921 gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac agtgtggctg
    13981 ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg gagagcgacg
    14041 atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac gatatgatga
    14101 ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag ttgggatcgt
    14161 tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta cttagcaaga
    14221 atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt taccgcttga
    14281 cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact gctaatgcgt
    14341 tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg tcgaagccgg
    14401 taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc aggaaatcgc
    14461 gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc aaatgccaga
    14521 tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa taatgggaat
    14581 cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc cagataagac
    14641 actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac ttaacgtcac
    14701 tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca tcatcgcaga
    14761 tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac
    14821 caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgcttgtc
    14881 accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt
    14941 cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac cttgtttcag
    15001 cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat
    15061 agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga ttaaggtaat
    15121 gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat gcgcatccag
    15181 tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca
    15241 ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat
    15301 aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac
    15361 tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt
    15421 ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa
    15481 gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc
    15541 tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt
    15601 gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt
    15661 caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt
    15721 gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag
    15781 taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat
    15841 cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt
    15901 ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt
    15961 caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta ccaagcccat
    16021 cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga
    16081 atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata aacgggaagt
    16141 gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg cggaatgtca
    16201 ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg
    16261 aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg cgatcgccgc
    16321 ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga
    16381 tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg
    16441 tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc
    16501 gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg
    16561 cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt ttcataaagg
    16621 tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta
    16681 ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc
    16741 tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat
    16801 gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa
    16861 ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca
    16921 gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg
    16981 tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta tagccaaata
    17041 acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat
    17101 ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg
    17161 ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc gttccttctt
    17221 tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa ctgtccagcg
    17281 gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat
    17341 gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc
    17401 ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc aacatttctg
    17461 cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag gtgagatctt
    17521 tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg taatacatcg
    17581 accacggtac attttctgca ccccacaatg tatccagata agtggacatc cgctgagtca
    17641 tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgtggtt
    17701 tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg
    17761 gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga
    17821 cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct ttcagccagg
    17881 cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg atcaacagta
    17941 aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt
    18001 tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag
    18061 ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct cccgtgatat
    18121 ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct
    18181 acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat tatgctgatg
    18241 ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc acagaataat
    18301 gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaataggggt
    18361 aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat agcgggagat
    18421 attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata cttaagaata
    18481 agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAJTVE010000084        18214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000084 DAJTVE010000000
VERSION     DAJTVE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..1094
                     /locus_tag="OO439_002710"
     CDS             18..1094
                     /locus_tag="OO439_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6317040.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1487..1828)
                     /locus_tag="OO439_002711"
     CDS             complement(1487..1828)
                     /locus_tag="OO439_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6317041.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1841..2713)
                     /gene="copD"
                     /locus_tag="OO439_002712"
     CDS             complement(1841..2713)
                     /gene="copD"
                     /locus_tag="OO439_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6317042.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2717..3091)
                     /gene="yobA"
                     /locus_tag="OO439_002713"
     CDS             complement(2717..3091)
                     /gene="yobA"
                     /locus_tag="OO439_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6317043.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3230..3460
                     /gene="holE"
                     /locus_tag="OO439_002714"
     CDS             3230..3460
                     /gene="holE"
                     /locus_tag="OO439_002714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6317044.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3562..4218
                     /locus_tag="OO439_002715"
     CDS             3562..4218
                     /locus_tag="OO439_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6317045.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4242..4904
                     /gene="exoX"
                     /locus_tag="OO439_002716"
     CDS             4242..4904
                     /gene="exoX"
                     /locus_tag="OO439_002716"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6317046.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4901..6961)
                     /gene="ptrB"
                     /locus_tag="OO439_002717"
     CDS             complement(4901..6961)
                     /gene="ptrB"
                     /locus_tag="OO439_002717"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6317047.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7170..7829)
                     /locus_tag="OO439_002718"
     CDS             complement(7170..7829)
                     /locus_tag="OO439_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6317048.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8156..8512)
                     /locus_tag="OO439_002719"
     CDS             complement(8156..8512)
                     /locus_tag="OO439_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6317049.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8579..8869)
                     /gene="yebG"
                     /locus_tag="OO439_002720"
     CDS             complement(8579..8869)
                     /gene="yebG"
                     /locus_tag="OO439_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6317050.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9003..10181
                     /gene="purT"
                     /locus_tag="OO439_002721"
     CDS             9003..10181
                     /gene="purT"
                     /locus_tag="OO439_002721"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6317051.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10237..10878)
                     /gene="kdgA"
                     /locus_tag="OO439_002722"
                     /gene_synonym="eda"
     CDS             complement(10237..10878)
                     /gene="kdgA"
                     /locus_tag="OO439_002722"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6317052.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10915..12726)
                     /gene="edd"
                     /locus_tag="OO439_002723"
     CDS             complement(10915..12726)
                     /gene="edd"
                     /locus_tag="OO439_002723"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6317053.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12961..14436)
                     /gene="zwf"
                     /locus_tag="OO439_002724"
     CDS             complement(12961..14436)
                     /gene="zwf"
                     /locus_tag="OO439_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6317054.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14774..15643
                     /gene="hexR"
                     /locus_tag="OO439_002725"
                     /gene_synonym="yebK"
     CDS             14774..15643
                     /gene="hexR"
                     /locus_tag="OO439_002725"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6317055.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15771..17213
                     /gene="pyk"
                     /locus_tag="OO439_002726"
     CDS             15771..17213
                     /gene="pyk"
                     /locus_tag="OO439_002726"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6317056.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17273..18115)
                     /locus_tag="OO439_002727"
     CDS             complement(17273..18115)
                     /locus_tag="OO439_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6317057.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4672 a         4575 c         4391 g         4576 t
ORIGIN      
        1 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
       61 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      121 cactaggtaa aatttcaaga agagatgcag taacgcaggc aattgaagca aatcattata
      181 tatacaaaaa ctactctccc gctgccttaa ttgaaaaact taaagggttc gactcattta
      241 ctatggcaga ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gtgtccagaa
      301 atacttataa aattcgggca aatcaactgg agacaataaa agaaaaattg ggagagattt
      361 tactgacaga aataaccact cgccatattg ccgagtttct tgatttgtgg attgaaggag
      421 ggaaaaacac aatggcagga tcaatgcgtt ctgtgttgtc tgatatgttt cgcgaggcca
      481 ttgttgaagg acgtatatct caaaatccag tcacgccaac aagagcaccg aaaatagtag
      541 ttacaagaga acgactgaaa ctaaagatat acaactgcat cagggaggca gcagatcaac
      601 ttccggcatg gttcccatta gctatggatt tagcccttgt aacaggacaa cgtcgcgaag
      661 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacgtc aggcaaatta
      721 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtctacggc
      781 ttggtacagt agttgaacga tgccgcctgg taagccgggg agattatcta atcagtgccg
      841 ggattagaaa aaacagccct gacggcagca ttcacccgga tggcctgaca aaaaaatttg
      901 tcgcagccag aaaattcaca ggtatccagt tcagtgaaaa cccaccaact tttcacgaga
      961 tcagaagcct ggctggacga ttgtacaaag aaacatgtgg agaagaattt gctcagcgtc
     1021 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1081 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1141 caaaaagacc ggaatacaga aattcgagta aatttcgggg aatttcgggg gagacatttg
     1201 caactaattg atttcaaatg caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1261 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1321 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1381 caataaaaag cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1441 actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1501 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1561 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1621 tatcgtttaa aggatactgc acgagggtac tcggattaat aacatacaaa gcattacccg
     1681 gtcggcaagt cagcatcacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     1741 aacgactgac ggtaatcacc tgcggtgcag ccagcgccgc tgcagaactg gtgagtaaca
     1801 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     1861 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     1921 acacaaacca gggtgccgca cgatcttcct cctgcctcat gcgcggtaca agaacataac
     1981 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2041 ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2101 cgcttgccag tacgccgatc accgcaaaat gcccgcacca tgaaaaacac atcagcgcct
     2161 gaatagcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2221 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2281 tcgccgttac cccttcattc agtgtcgcat gccccattcc cgccagcaga ataaattgcg
     2341 cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2401 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2461 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2521 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2581 gcagaaaacg cttcgccagc aagcgacgaa tcgtcaaggg ggccagccag gcaccgtaca
     2641 ttgcgaagcc aaaaaccagc atcagcgagg tgaaatggat aaatcgcagc gcgatccagg
     2701 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     2761 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     2821 gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc cggtaatgtt ttaatatttt
     2881 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttccacacct tccgagaagt
     2941 ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3001 ttaaatgcgc atgtgcccag acgaaaggtg tcaccaacga agtggtcagg attgccagcg
     3061 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3121 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3181 ctggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3241 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3301 ggtggcattt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3361 gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3421 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gggttacact
     3481 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcacccat aatttgcagt
     3541 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3601 cgtaaaacct cactcaccga acaagtagct catcatctgg agtttgtgag ggccgccgcc
     3661 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     3721 cgacgtgccc ttcctgcccc acccgatctc tcactgttag acccgctttg ttatgccgca
     3781 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     3841 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggatctacca tcagagtcat
     3901 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accggaaggt
     3961 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4021 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgctgtgctg
     4081 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4141 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4201 atcattccat tacgctaggc tttttcggcc tggagtatgc catgttgcgc attatcgata
     4261 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4321 gaaaaatcgt caaccccatg agccacctgg tgcgccccga tcgtcctatt agtccacaag
     4381 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4441 tgatcccaca ctattacggc agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4501 gcgtactgcc tgagatgccc ggtgagtgga tttgcaccat gaaactggcc cgtcgcttgt
     4561 ggccaggaat caagtacagc aatatggcgt tatataaaac acggaagctc aatgtacaga
     4621 cgccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4681 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     4741 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     4801 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     4861 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagcgg
     4921 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     4981 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5041 gatggttatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt
     5101 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5161 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcgggttac
     5221 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5281 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5341 caacgcccat caacatgccc cccgcactcc cgcccatcgc ataacaaagc gaaggagagc
     5401 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5461 tcttcagaaa ttttccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5521 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5581 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5641 gaaaatgttt gcgatggtag accaacgaaa ccggaacttc gacgccatca cgggcgacta
     5701 tccacaggtg ttcactgcgg taattcgccg catcaaaacc aggaacttcc gtttgtttta
     5761 atacacgacg ctcatcggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     5821 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     5881 cataggctgg atcatcaaag gcaataccaa tgacttcccg ggtcttgcgg ttaatttggc
     5941 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6001 acccttccag catgatgttt tcgcatggcg gaattaactc ttcccactgt tgctcatcac
     6061 gcatacgggt acggtataag ccaaagtttt tgccgtggcg gttggaacgc agataaaaac
     6121 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6181 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6241 aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag gctgacgtaa taggtatcgt
     6301 ctttttcttc gtagatcagt ttatcttgcg atgctggcgt accgatggcg tgacgccaga
     6361 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6421 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttaccagttt
     6481 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6541 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6601 cgcgcttatt ggcatcgagc aatatttccc actcatccca ctcttcactg aatgccgatt
     6661 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     6721 ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg gtcgatgatt tcctttaaga
     6781 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     6841 gcaggtagtc caggacttct ggctgagaac gcgtatcatc ccgcagccag tagtaattat
     6901 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     6961 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
     7021 agcgaaacag gggagtcatt gcttagatga tgacaggtaa tggcacggat atcgaatgtt
     7081 atgcagacag agaaactcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata
     7141 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
     7201 agatcgcgtt caatgccgtc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
     7261 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcaact caggaaatag
     7321 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
     7381 tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
     7441 tgctggtcga ttgccgcacg ttctttggca tcaatatgac catcactttt agcggcaaag
     7501 accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg gcgtactttg cgcgccaaac
     7561 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
     7621 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
     7681 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
     7741 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
     7801 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
     7861 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
     7921 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
     7981 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
     8041 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctgggg
     8101 cggacaaggc actcgcgccg catccaccaa cggtcaatcg aattatcaga agccattaac
     8161 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
     8221 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
     8281 gcaaactgac ccaagccacg ggatcggcct gaccgacaat tttttgatca tctgcccaac
     8341 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gcagcatcca
     8401 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
     8461 atgcgcaggc agaaaccaac aacagcccta aaaacgcccc tctttttttc atgtttttct
     8521 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
     8581 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta
     8641 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
     8701 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca
     8761 gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca
     8821 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
     8881 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
     8941 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
     9001 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
     9061 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
     9121 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
     9181 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
     9241 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
     9301 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
     9361 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc
     9421 ttttccgcga ggcggttgct gacattggct atccctgcat tgtaaaaccg gtgatgagct
     9481 cttccggcaa ggggcagacg tttattcgtt ctgcagagca acttgctcag gcatggaagt
     9541 acgctcagca aggcggtcgc gccggagcag gccgcgtaat tgttgaaggc gtcgttaagt
     9601 ttgacttcga aattaccttg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
     9661 cggtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
     9721 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
     9781 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
     9841 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctttcagagt
     9901 ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt cagtatggtc
     9961 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    10021 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    10081 gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag tgttgttgac gccattgaac
    10141 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta
    10201 caaaaatgcc cgatcgtaga tcgggcgttt tgacttttac agcttagcgc cttctacagc
    10261 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
    10321 cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc
    10381 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    10441 cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta
    10501 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaattagag gaatagtccc
    10561 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    10621 cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc
    10681 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    10741 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ctgcgtgttc
    10801 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    10861 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    10921 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    10981 ttcacgtcct gtccccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    11041 cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat
    11101 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    11161 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    11221 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    11281 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    11341 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    11401 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    11461 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    11521 gagtgatttt tccgcccctt cccgccagtc ctgttcacca ttattcagcc atggttcaag
    11581 ggtataacga gacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    11641 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    11701 ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa
    11761 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    11821 gttagtggaa ccaccggtcg ccagcagtgc aacgataccg ttcaccacca ctttctcatc
    11881 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    11941 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    12001 tggcaactgc atccccataa attccaccac catctggttg gtgttggcag taccgtagaa
    12061 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    12121 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    12181 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaagcgaca gggctgccat
    12241 cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt
    12301 atgggacagc cccaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc
    12361 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    12421 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    12481 ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    12541 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    12601 gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata
    12661 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcata acaattgtgg
    12721 attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt
    12781 cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    12841 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    12901 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata
    12961 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    13021 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    13081 aatggagtct acccatttcc aggcttcttc cacttcatca cgacgtacaa acagtgcctg
    13141 aataccacgc atggtttcca gcagcagacg ttcataggca tccgccagat gcgtctgatt
    13201 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    13261 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    13321 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    13381 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    13441 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    13501 attgctgctc ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    13561 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg
    13621 gcgcagggac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    13681 tggcggagac atcgcaatca tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg
    13741 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    13801 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    13861 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    13921 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    13981 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    14041 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt
    14101 gatacgattt ttttgatcca gcatcgcgcc gagacggttg aatgcagcag tgtcattgac
    14161 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    14221 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    14281 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    14341 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    14401 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    14461 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg
    14521 agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt
    14581 gcggcaaaac tgataaaaaa atcatcgttt tccattcggt aaaaatctga cactgatcat
    14641 gttatgaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    14701 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    14761 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    14821 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    14881 tattgctgct atggcactgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    14941 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaga gtctggcgaa
    15001 tggcactccc tatgttaatc gcaatgtcaa tgaagatgac agcgttgaat catacacagg
    15061 gaaaatattt gagtccgcaa tggcaacgct tgatcatgtc cgtcattcac tggataaatc
    15121 tgccatcaac cgcaccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    15181 aggctcttca gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    15241 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    15301 agacgtggtg gtgctgattt ctcacactgg aagaacaaaa aatctggtcg agctggcgca
    15361 gctggctcgc gaaaacgacg caatggtgat tgccctcacc tctgcgggta ccccgctcgc
    15421 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    15481 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    15541 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    15601 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc
    15661 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    15721 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccagaa
    15781 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc
    15841 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    15901 ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    15961 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    16021 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    16081 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    16141 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    16201 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    16261 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    16321 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    16381 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    16441 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    16501 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    16561 gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    16621 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    16681 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgcag
    16741 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    16801 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    16861 ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg gcgattatta
    16921 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    16981 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    17041 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    17101 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    17161 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taatctcacc
    17221 tgccccggcc gctgatggcc ggggtattta gcaacacata aattaggact acttattcaa
    17281 tccaatcata atcacagccc cctgtaaaat gaatacaggg atagcctgaa tggagatcta
    17341 aatagcttgc aagacgtcgc atacccgctt cctctgcaac actatgtcca ataactgcca
    17401 tcggtaagcc actatctttg gagtattgtg tctgcaccca tgttttaatt gaatcgttaa
    17461 tcgtaatata ataatcagcc tgatattcca tatattgtat tggatcacaa aaacagcctg
    17521 ccccaatgcc aacagttctg actactctgg ctttatctcc ataaaattga atgccaggaa
    17581 gattatatat tttcaaattt ttggcaaaac gtttggtaat gttaatggca ctgtcagggt
    17641 ctatagcata tacacagtac atctcatcag aagcaacaat atttgtaaca tccagttcta
    17701 actgctgagc caaagcctta gacattccga acgtaggctc tcgatccatg acatcatggc
    17761 aacgaatgat taccaaatta tttttatgca aaaactcctt tttttgctca attattttta
    17821 aataactgag ttcaccgcga gtataatcca ttatttttct ggcccatgaa aactcaagat
    17881 cgtcaccctc cagatcagca taagaataaa atgtaggctc atgtgtaata atgatattga
    17941 caccttcaaa aacagccttt cttaaaacat ccattgaggc aatccagcag gtcccaattt
    18001 tattaataag tgtcgcctca gcaccaaaaa taattttatc aacagttggt tgcgaaacag
    18061 caaccattga atttaaaaaa caattaagat ccgcggctgt tttaatatga tgcattcaac
    18121 atccttcaca ggtttcatca gcaataaaat aatttcaaca tttctgggaa acgttttgcc
    18181 catgcagact cagagtgggt atcacctccc cata
//
LOCUS       DAJTVE010000085        17846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000085 DAJTVE010000000
VERSION     DAJTVE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO439_002728"
     CDS             complement(<1..304)
                     /locus_tag="OO439_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6317058.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(344..481)
                     /locus_tag="OO439_002729"
     CDS             complement(344..481)
                     /locus_tag="OO439_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317059.1"
                     /translation="MLFLYYAVERKVVSKGLRSREMADAGNFGGSVPERKRNTVLSQP
                     L"
     gene            complement(514..1032)
                     /locus_tag="OO439_002730"
     CDS             complement(514..1032)
                     /locus_tag="OO439_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6317060.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OO439_002731"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OO439_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6317061.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OO439_002732"
     CDS             2264..2488
                     /locus_tag="OO439_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317062.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OO439_002733"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OO439_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OO439_002734"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OO439_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6317063.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OO439_002735"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OO439_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6317064.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OO439_002736"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OO439_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6317065.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OO439_002737"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OO439_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6317066.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OO439_002738"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OO439_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6317067.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OO439_002739"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OO439_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OO439_002740"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OO439_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6317068.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14044
                     /gene="tssH"
                     /locus_tag="OO439_002741"
                     /pseudo
     CDS             11267..14044
                     /gene="tssH"
                     /locus_tag="OO439_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14041..14784
                     /gene="tagO"
                     /locus_tag="OO439_002742"
     CDS             14041..14784
                     /gene="tagO"
                     /locus_tag="OO439_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6317069.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14789..16201
                     /gene="tssA"
                     /locus_tag="OO439_002743"
     CDS             14789..16201
                     /gene="tssA"
                     /locus_tag="OO439_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6317070.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16220..>17846
                     /gene="tssM"
                     /locus_tag="OO439_002744"
     CDS             16220..>17846
                     /gene="tssM"
                     /locus_tag="OO439_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6317071.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4151 a         4894 c         4876 g         3925 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgttcacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agcaggataa taccggtgcc gaaccggctg atgaggccga
    12901 cagagaacaa ccggaagaga ccgctgaaac agtttccccg gtacagcggc tggcccagct
    12961 cactgccgaa ctggacgccc tgcataacga ccggttgctg gtctccccgc acgtcgataa
    13021 aaaacagatt gcggcggtga ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca
    13081 gaatgaaatg tcggtcatca ccgacctgac gaaatggctg ggtgacacca tcaaaggcca
    13141 ggacctggcg attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtcg
    13201 tccgggacgc ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga
    13261 aaccgtcctg caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa
    13321 tatgtccgaa ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta
    13381 cgttggctac ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt
    13441 agtactgctc gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc
    13501 gttcgataag ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt
    13561 cttcttcctg acgtccaacc tcggctacca ggtaatagtc gagcatgccg atgacccgga
    13621 aaccatgcag gaagtacagt atccggtgct ggccgacttc ttcaaacctg ccctgctggc
    13681 gcgtatggaa gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc
    13741 cggaaaactg gcccgtctgg ataacgtgct gcgcagtcgc tttggtgtag aagtggtcat
    13801 tgaaccggaa gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag
    13861 gatgctggaa tcggtcatcg atggcgacat gctaccgccg ctctcgctgc tgctgttgca
    13921 gaaaatggcg gctaacacgg cgattgcccg gattcggttg tcggcagtgg acggcgcatt
    13981 tacggcagac gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt
    14041 atgagcaggt tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc
    14101 ttacccgcca ctgccgcgcc cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc
    14161 gccaccacga ccccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg
    14221 gagtccgcgg cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg
    14281 tcagggtttg acggggcgct ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc
    14341 acagagcagg aaaaacgccg cgagggcaac accacagaac tgctggtgac gcaggtgccg
    14401 ggggagcgcc cgacggtggt gataaccacc ccggctatcg gtcatgttcc gccacgcccg
    14461 gtgctgatgt tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct
    14521 ctggatgtcc atgatatcgc cgtcactctg aatgccgaca accgggcatt gcgcagccac
    14581 tggtttgtgc gcgagaacgg caccctgctg gagtccagcc gggggttatc cgggattgat
    14641 gaaattaagc aactgttcgg cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc
    14701 gccggaaagc tgacctttaa cattgacggg ctggcaaggg cgattgctcc cctgcgcgat
    14761 gcctgccact gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg
    14821 gatatcgcac ctgctggaaa acctgccgga agagaaactg gcatcggcgc tgaaagatga
    14881 caacccgaac tgggagtata tcgacggcga aatcgtcaag ctgggatcgc tggcccacag
    14941 tcaactggat atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa
    15001 ggattttcgt ctgctggcgc atctgctaag aaccctgcaa cacgccggtg atcctcatct
    15061 cgcattgcgc ctgctggcgc tgtatgtgga gcattactgg gcggtggctg cgccgcagaa
    15121 tatggcgcat aaaaaacgct ttgccagcca ggtcattaaa cgctttgaaa caggtattga
    15181 agttttttca cagaacgccg ccacagcgca gcgcgatgcg ctgtcagggg aactggcaaa
    15241 actggcgcaa tgctggcagt cacataacgc cccggaactg gcacaggcta ctgatgatct
    15301 ttttgccctg taccagcgtg cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc
    15361 ggcctccggt agttcaccac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag
    15421 tgctccggct ccccaaatcg tcatcgacag tcacgacgac aaagcctggc gcgacacgct
    15481 gttaaaagtg gcggccattt tatgtgaacg ccagccggat tcaccgcagg gctaccgtct
    15541 gcgccgccat gcgctttggc agaacatcac cagtacgccc caggcggaaa gcgatagccg
    15601 tactccactg gctgcggtct ccgccgatat ggtggcagat taccatgctc agctcggtag
    15661 tgccgatatg gcgctgtggc agcaggtgga gaaaagcgta ctgctggctc cttactggct
    15721 ggatggtcac tgcctttctg cacagacggc gctgcgtctg ggttacaaac aggtggcaga
    15781 cgccatccac gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct
    15841 gtttaatgac cacacaccgt ttatcagtga gcagacgaaa caatggctgg cagcttcgcc
    15901 cgacgccaaa gttgcaccgg ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg
    15961 ttttgctgag cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga
    16021 tccccgcgat cagtttcacc gccagtacct tgccgcacag ttgacggaag aggcggggct
    16081 ggtacagctt gcgcagcagc agtaccggat gttgttcagg atgggattgc agatgatggt
    16141 ggcagactgg gagccgtccc tgcttgagca gctagaacaa aaattcacgg cagaacaata
    16201 aaagagaaca ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa
    16261 atcagccctg cgaccggcga tgccccggtt taaagtctct gctttctggc tgctgatact
    16321 ggcgtggatt tttctgctgg tgtggatctg gtggaaaggc ccgatgtgga cgctgtatga
    16381 agaacagtgg ctcaaaccac tggcgaaccg ctggctggca acggcggcgt gggggattat
    16441 tgccctggtg tggcttaccg tccgggtgat gaagcgcctg caacagcttg aaaaaatgca
    16501 aaagcaacag cgcgaggaag ccgttgatcc gctcagcgtg gaactgaacg cccagcagcg
    16561 ttatcttgac cgctggctgc tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg
    16621 gcagttgccg tggtatatgg tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg
    16681 cgaggggttt ccgtccgaca ttatttatgc cccggagggc gcacgcggcg cagaacaacg
    16741 cctgtacctc acgccccatg tcggtaaaca ggcggtgatc tttgatatcg acggcacact
    16801 ctgcgctccc gctgatgcgg atatcctgca tcgccgtctg tgggaacatg ccctcggctg
    16861 gctgaaagaa aagcgcgcgc gccagccgct gaacgggatt attctgacac tcgatttacc
    16921 cgatctgctg accgcagaca aacgccgccg cgagcatctg ttacagacgc tgcgcagccg
    16981 cttacaggat atacgccagc atcttcactg ccagttaccg gtttacgtgg tactgacccg
    17041 gcttgattta ttacagggtt ttgccgccct gttccagtca ctcaacagac aggatcgcga
    17101 tgcgattctg ggcgtcacgt tcacccgccg tgcccatgaa aatgatgact ggcgaacaga
    17161 gttgaatgct ttctggcaga catgggtgga tcgaatgaat ctggcgttgc cggatctgat
    17221 ggtcgctcag actcacaccc gcgcgtcttt attcagtttt tcccgccaga tgcagggaag
    17281 ccgtgaaccg ctggtgtcac tgcttgaggg tctgcttgat ggcgaaaata tgaacgtgat
    17341 gctgcgtggt gtctatctca cctcttcgct tcagcgtgga cagatggatg atatattcac
    17401 ccagtctgcc gcccgccagt accggctggg caataaccca ctggcgtcct ggcccctggt
    17461 ggacaccgcg ccttatttca cccgcagcct gttcccgcag gcattactcg cagagcctaa
    17521 tctggcaaca gagagccgcg cctggctgat acgttcccgt cgccgcctga cggttttctc
    17581 tgccacaggc ggcgtggcag cactgctgct catcaccggc tggcatcact attacaacgg
    17641 taactatcag tccggcatca ccgtgcttaa gcaggccaaa gcctttatgg acgtgccgcc
    17701 tccgcagggg gaagatgact ttggcaacct gcaactgccg ctcctgaacc cggtacgcga
    17761 tgccacactg gcctatggcg actggggcga ccgcagccgt ctggccgata tgggactgta
    17821 ccagggacga cgtatcgggc cttatg
//
LOCUS       DAJTVE010000086        17767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000086 DAJTVE010000000
VERSION     DAJTVE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(47..1021)
                     /gene="cysB"
                     /locus_tag="OO439_002745"
     CDS             complement(47..1021)
                     /gene="cysB"
                     /locus_tag="OO439_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6317072.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1231..3828)
                     /gene="topA"
                     /locus_tag="OO439_002746"
     CDS             complement(1231..3828)
                     /gene="topA"
                     /locus_tag="OO439_002746"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6317073.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4208..4459
                     /locus_tag="OO439_002747"
     CDS             4208..4459
                     /locus_tag="OO439_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6317074.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="OO439_002748"
     CDS             complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="OO439_002748"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6317075.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5764..6522
                     /locus_tag="OO439_002749"
     CDS             5764..6522
                     /locus_tag="OO439_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6317076.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6519..7109
                     /gene="cobO"
                     /locus_tag="OO439_002750"
     CDS             6519..7109
                     /gene="cobO"
                     /locus_tag="OO439_002750"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6317077.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="OO439_002751"
     CDS             complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="OO439_002751"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6317078.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8234..9348)
                     /locus_tag="OO439_002752"
                     /pseudo
     CDS             complement(8234..9348)
                     /locus_tag="OO439_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9376..9996)
                     /locus_tag="OO439_002753"
     CDS             complement(9376..9996)
                     /locus_tag="OO439_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6317079.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9993..10874)
                     /gene="yciV"
                     /locus_tag="OO439_002754"
     CDS             complement(9993..10874)
                     /gene="yciV"
                     /locus_tag="OO439_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6317080.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11012..11056
                     /gene="trpL"
                     /locus_tag="OO439_002755"
     CDS             11012..11056
                     /gene="trpL"
                     /locus_tag="OO439_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6317081.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11033..11126
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11148..12710
                     /gene="trpE"
                     /locus_tag="OO439_002756"
     CDS             11148..12710
                     /gene="trpE"
                     /locus_tag="OO439_002756"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6317082.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12710..14305
                     /gene="trpD"
                     /locus_tag="OO439_002757"
     CDS             12710..14305
                     /gene="trpD"
                     /locus_tag="OO439_002757"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6317083.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14306..15667
                     /gene="trpCF"
                     /locus_tag="OO439_002758"
     CDS             14306..15667
                     /gene="trpCF"
                     /locus_tag="OO439_002758"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6317084.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15679..16872
                     /gene="trpB"
                     /locus_tag="OO439_002759"
     CDS             15679..16872
                     /gene="trpB"
                     /locus_tag="OO439_002759"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6317085.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16872..17678
                     /gene="trpA"
                     /locus_tag="OO439_002760"
     CDS             16872..17678
                     /gene="trpA"
                     /locus_tag="OO439_002760"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6317086.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4246 a         4721 c         4451 g         4349 t
ORIGIN      
        1 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
       61 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      121 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      181 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      241 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      301 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      361 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      421 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      481 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      541 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa tcagcattgc
      601 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      661 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      721 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      781 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
      841 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
      901 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
      961 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1021 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1081 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1141 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1201 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1261 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1321 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1381 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1441 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1501 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1561 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1621 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1681 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1741 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1801 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     1861 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     1921 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     1981 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2041 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2101 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2161 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2221 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2281 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2341 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2401 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2461 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2521 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2581 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2641 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2701 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2761 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     2821 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     2881 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     2941 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3001 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3061 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3121 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3181 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3241 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3301 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3361 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3421 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3481 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3541 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3601 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3661 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3721 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3781 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     3841 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     3901 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     3961 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4021 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4081 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4141 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4201 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4261 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4321 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4381 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4441 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4501 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4561 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4621 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4681 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4741 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4801 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     4861 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     4921 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     4981 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5041 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5101 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5161 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5221 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5281 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5341 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5401 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5461 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5521 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5581 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5641 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5701 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5761 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     5821 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     5881 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     5941 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6001 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6061 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6121 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6181 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg tcagggacga
     6241 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6301 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6361 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6421 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6481 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6541 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6601 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6661 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6721 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6781 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     6841 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     6901 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     6961 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7021 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7081 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7141 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7201 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7261 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7321 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7381 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7441 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7501 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7561 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7621 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7681 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7741 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7801 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     7861 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     7921 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     7981 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8041 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8101 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8161 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8221 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8281 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8341 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8401 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8461 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8521 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8581 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8641 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8701 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8761 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     8821 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     8881 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     8941 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9001 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9061 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag gctgcatcca
     9121 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9181 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9241 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9301 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9361 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9421 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9481 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9541 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9601 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9661 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9721 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9781 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
     9841 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
     9901 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
     9961 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10021 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10081 agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10141 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10201 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10261 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10321 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10381 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10441 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10501 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10561 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10621 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10681 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10741 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10801 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    10861 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    10921 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    10981 tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11041 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11101 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11161 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11221 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11281 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11341 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11401 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11461 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11521 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11581 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11641 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11701 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11761 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    11821 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    11881 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    11941 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12001 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12061 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12121 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12181 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12241 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12301 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12361 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12421 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12481 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12541 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12601 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12661 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12721 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12781 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    12841 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    12901 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    12961 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13021 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13081 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13141 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13201 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13261 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13321 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13381 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13441 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13501 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13561 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg ggctgaaagt
    13621 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13681 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13741 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13801 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    13861 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    13921 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    13981 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14041 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14101 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14161 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14221 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14281 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14341 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14401 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14461 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14521 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14581 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14641 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14701 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14761 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    14821 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    14881 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    14941 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15001 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15061 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15121 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15181 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15241 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15301 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15361 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15421 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15481 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15541 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15601 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15661 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15721 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15781 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    15841 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    15901 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    15961 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16021 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16081 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16141 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16201 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16261 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16321 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16381 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16441 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16501 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16561 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16621 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16681 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16741 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16801 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    16861 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    16921 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    16981 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17041 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17101 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17161 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17221 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17281 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17341 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17401 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17461 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17521 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17581 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17641 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc cccctgccgc cagttccgct
    17701 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17761 cttttta
//
LOCUS       DAJTVE010000087        17450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000087 DAJTVE010000000
VERSION     DAJTVE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..502)
                     /locus_tag="OO439_002761"
     CDS             complement(<1..502)
                     /locus_tag="OO439_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6317087.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OO439_002762"
     tRNA            complement(688..772)
                     /locus_tag="OO439_002762"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OO439_002763"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OO439_002763"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6317088.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO439_002764"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO439_002764"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6317089.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OO439_002765"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OO439_002765"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6317090.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OO439_002766"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OO439_002766"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6317091.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OO439_002767"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OO439_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6317092.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OO439_002768"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OO439_002768"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6317093.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OO439_002769"
     CDS             7117..8619
                     /locus_tag="OO439_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6317094.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO439_002770"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO439_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6317095.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO439_002771"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO439_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6317096.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OO439_002772"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OO439_002772"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6317097.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OO439_002773"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OO439_002773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6317098.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OO439_002774"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OO439_002774"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6317099.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OO439_002775"
     CDS             complement(16186..17382)
                     /locus_tag="OO439_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6317100.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4485 a         4337 c         4453 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgcccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgccac
//
LOCUS       DAJTVE010000088        17335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000088 DAJTVE010000000
VERSION     DAJTVE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..186)
                     /gene="adrA"
                     /locus_tag="OO439_002776"
                     /gene_synonym="yaiC"
     CDS             complement(<1..186)
                     /gene="adrA"
                     /locus_tag="OO439_002776"
                     /gene_synonym="yaiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="catalyzes the conversion of 2 GTP into c-di-GMP;
                     adrA overexpression induces cellulose biosynthesis, cell
                     adherence to abiotic surfaces and swimming and swarming
                     motility; AdrA acts post-transcriptionally on the bcsABZC
                     operon activating cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6317101.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVS"
     gene            complement(288..608)
                     /gene="psiF"
                     /locus_tag="OO439_002777"
     CDS             complement(288..608)
                     /gene="psiF"
                     /locus_tag="OO439_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6317102.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(727..2142)
                     /gene="phoA"
                     /locus_tag="OO439_002778"
     CDS             complement(727..2142)
                     /gene="phoA"
                     /locus_tag="OO439_002778"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6317103.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2243..2503)
                     /gene="iraP"
                     /locus_tag="OO439_002779"
     CDS             complement(2243..2503)
                     /gene="iraP"
                     /locus_tag="OO439_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6317104.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2685..2888)
                     /locus_tag="OO439_002780"
     CDS             complement(2685..2888)
                     /locus_tag="OO439_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317105.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            2966..4060
                     /gene="ddlA"
                     /locus_tag="OO439_002781"
     CDS             2966..4060
                     /gene="ddlA"
                     /locus_tag="OO439_002781"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6317106.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4084..4296)
                     /locus_tag="OO439_002782"
     CDS             complement(4084..4296)
                     /locus_tag="OO439_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6317107.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4556..4864
                     /locus_tag="OO439_002783"
     CDS             4556..4864
                     /locus_tag="OO439_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6317108.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4923..6017)
                     /gene="yaiW"
                     /locus_tag="OO439_002784"
     CDS             complement(4923..6017)
                     /gene="yaiW"
                     /locus_tag="OO439_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6317109.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6030..7250)
                     /gene="sbmA"
                     /locus_tag="OO439_002785"
     CDS             complement(6030..7250)
                     /gene="sbmA"
                     /locus_tag="OO439_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6317110.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7602..8759
                     /gene="ampH"
                     /locus_tag="OO439_002786"
     CDS             7602..8759
                     /gene="ampH"
                     /locus_tag="OO439_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6317111.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8760..9383)
                     /gene="iprA"
                     /locus_tag="OO439_002787"
     CDS             complement(8760..9383)
                     /gene="iprA"
                     /locus_tag="OO439_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6317112.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(9471..12359)
                     /locus_tag="OO439_002788"
     CDS             complement(9471..12359)
                     /locus_tag="OO439_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6317113.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            12885..13859
                     /gene="hemB"
                     /locus_tag="OO439_002789"
     CDS             12885..13859
                     /gene="hemB"
                     /locus_tag="OO439_002789"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6317114.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(13965..14816)
                     /gene="tauD"
                     /locus_tag="OO439_002790"
     CDS             complement(13965..14816)
                     /gene="tauD"
                     /locus_tag="OO439_002790"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6317115.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(14813..15640)
                     /gene="tauC"
                     /locus_tag="OO439_002791"
     CDS             complement(14813..15640)
                     /gene="tauC"
                     /locus_tag="OO439_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6317116.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(15637..16404)
                     /gene="tauB"
                     /locus_tag="OO439_002792"
     CDS             complement(15637..16404)
                     /gene="tauB"
                     /locus_tag="OO439_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6317117.1"
                     /translation="MLQISHLYADYGGKPALEDINLTMESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(16417..>17335)
                     /gene="tauA"
                     /locus_tag="OO439_002793"
     CDS             complement(16417..>17335)
                     /gene="tauA"
                     /locus_tag="OO439_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6317118.1"
                     /translation="IAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWRKFD
                     SGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTISKP
                     EDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPAIIAAWQRGDIDGAYV
                     WAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQ
                     PYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAII
                     DTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4299 a         4497 c         4195 g         4344 t
ORIGIN      
        1 cgaaaccagc gttgaagcaa tcggtaagaa catgccagcc aggccaaccg cacggggtag
       61 tctgacgcga cgggcgaagc gcagcccgga ccgctgatgt tcgttttgag gagttaaagg
      121 aggttcctcc ccatgcgccg ccgctttttt gaaaaagttt tcatcattca ttatttttgg
      181 gaacattcag aaacaatttt cccaaattat agagacgggg ctaaaggcag aagtatgaaa
      241 tttcgggtga gccactttcg tagctcaccc gttagcacta tcaggtatca ggcggctttc
      301 ttgaggcagg cactcataaa cttattacga tcatcacctt tcagagattg ttgcgttgct
      361 tgattattac attcgcgcat cttttgctgc tgtggcgtca aacttttttc accaggcgca
      421 gacttgctgt tcttcaggca atcactcatg taggtcttac gagcatcccc tttcaacgcc
      481 tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt gttgtggggt taaggttctc
      541 tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa gcaaggtaac cagtaatgtt
      601 attttcatag caccatccct cttcatgttt taaccatgag cgtttgcgcc cgtgatctgc
      661 cattaagtct ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc actgccgggc
      721 gcggttttat ttcagcccca gagcggcttt catggtgtag aagagatcgg tctggtcggt
      781 cagtccaaca acattggcgg catgcggacc atacgccgca atacgcaact gactgccggt
      841 atgttcttgt gaatcctctt cggaattccc gtaactcatc accatcactg cgccatcttt
      901 agtatttagc gcctgagtga ggcccggcgc tttggtatcc ggcgcaacaa cctggctggc
      961 gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc tcctttttag cgaattccag
     1021 cgcccgttgt acggcttcat cgagatcgac cgtctcgcca atttgcccac aaggattcgc
     1081 agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc aggaaaaatc ctttctcatt
     1141 tttactcaac aattcaatgg ttttgtcggt catctgtgcc agggttggta cactgtcatt
     1201 acgttgcgga ttaggcgtac aggtgactgc gggcttatcg atattgccgt ggtacgttgc
     1261 tttcggccct agccagcgca ctggcatatt gccgtcagca aacaggccaa gcaggggttt
     1321 ttgctgattc gcttccgtca ccgaattcag tgaggcagca tcgttcacca actgataacc
     1381 acgcgcctgt gcctgttcac gcagcgtttt tccctgccat tcaccagcgg ttgccgtttc
     1441 agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca cgagcgttaa gcagctgttc
     1501 ggtaatcgat ccttttccgc ctttttccag agcgttaccc ggacattttt cactggtcgc
     1561 gctcggaccg tagcatttgc gcgaagtcac atgtgccacc agcgcagcgg gcgtggcatc
     1621 ctgcaactct gcggtagaaa cgttaccggt cgccagacct gcggcttttg ccatttccag
     1681 aatcgttggg tgatcttttt cgtgaatatc gacgcccagc gcgccgttat aggttttgac
     1741 accggttgac caggcggttg ctgatgcagc cgagtcggtg acgtagtccg gtttgccagt
     1801 ttttttattc agcgcatagt gagtgtattg cccggtaagc ggtaaggcat cgataccttt
     1861 aaaaaagccg cccgcacctt cggcataatt acgtgcggca gtaatttccg aatcccccat
     1921 cccatcgcca atcagcaaaa taatattttt tgcaggttta tcgctaagag aatcacgcag
     1981 agcggcggtc tgatcactcg ttaagcggcg agcaccgccg ggtgcagtaa tatcgccctg
     2041 agcagcccgg ttttccagaa caggcatttc tggtgtccgg gcttttgtca caggggtaaa
     2101 cagtaacggt aagagtgcca gtgcaatagt gctttgtttc actttatttt ctccatgtac
     2161 aaatacatta aaaaataaaa acaaagcgac tataagtctc ggccgtgaca actttatgac
     2221 agctgttgaa aagattaact ttttactgac gaggatgctt caataacttc tttacgtaat
     2281 cgcgcagcag ctccgtatcg tcgtcaggaa tgctggcatc gggctttacc tcgtacagcg
     2341 ccccctctac ctgatcaatc aaccgctgtt ggtcattttg cgccatattg cgaagcattg
     2401 cagtgacgat aatctccaaa gcttctacct gcgcacacag ttctttcgac tcttcttctt
     2461 tttgggcaag cttaaataac aactcagcaa tgagattttt catgtctgta tttccttatc
     2521 caaagtatgg agaagttaac attatgtttc acaacttcat agtgtctgga agtattattt
     2581 ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt gattaccagc gaaacgtttc
     2641 tcttatttaa ataattaccc acacaaaata taggctttta attatcaacc tttattataa
     2701 ataaaagtta tgaaatatat tctcttcgtt aacttataat taagagaaaa aaactcaccg
     2761 cattgccatt taagccagga ataaattagc ttcccgttca tcatttttgt gatctcgcgc
     2821 acatctttga gataacgcac tttcttcacc agtacgttgc acgctttgtc agcagaagat
     2881 tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt
     2941 gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg
     3001 taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg atgccattga
     3061 taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat ggcacgtcag
     3121 cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt tgcgcccttc
     3181 ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa
     3241 cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta cgctgggcga
     3301 agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag gttctgatgt
     3361 tctggcttca gcagcctgta tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg
     3421 gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc
     3481 cgaagtggag tctaaactgg ggttaccact gtttgtaaaa ccggctaatc agggctcttc
     3541 tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca attgccgtcg atctggcgtt
     3601 cgagttcgat cataaagtga tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc
     3661 agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac tcaccagcga
     3721 tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg tagttccggc
     3781 agccattgca ccagagatca acgataagat ccgggcgatt gccgttcagg cttatcagac
     3841 gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga acgaagtggt
     3901 gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc cgaagctgtg
     3961 gcaagccagc ggtctgggtt acaccgatct gatcacacgt ttgattgagc tggcgctgaa
     4021 gcgtcacgct gcggataacg cattgaaagc cacaatgtaa tattttaacg cccgataata
     4081 ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaacc ccgccagcca
     4141 gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa
     4201 tcccagtaac cccaccacgc cattaagaat gaatataagg ccaatggcga acgcatagta
     4261 gtgccagtca cggcggattt ttacaggcag attcatggca gctccgttaa cgaaaaacaa
     4321 catccttgca tattctgtaa caaccgtctg tggtgtagac aacattttcg taattgttaa
     4381 taaatttaac ttaaggttgc cattttgtaa ctcacaacga agattcacgc ttcgggaata
     4441 ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct gtcagacttg
     4501 ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg tttttatggc
     4561 tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc
     4621 tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg cgcaattgct
     4681 gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg
     4741 tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt ttcgggctga tcccgttttt
     4801 agtaggctgc ctcatttttg cagtggtggc gctatggctg cactggcgac atcgccgcca
     4861 gtaagattta cgttgccaga tacggcctaa aacgccttat ctggcatgac attcctgttt
     4921 gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta
     4981 gtcaggttgc gggtgatttt cgggctttcc agttgaatgc caggcaacat ttcgcggggt
     5041 agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag cgccgtctcc
     5101 tcaaaagaga agctgtcacc tttctctaac tgacggcgaa tttcgctgtc gctcatcccc
     5161 agttttcccg ccagtttgcg cgttgccagt tcggttttcc cgggttcttt actgtcgtaa
     5221 cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac tgcgttctga
     5281 aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt
     5341 gcgctatagc tggcgggata attcagtaaa tggtaagtac caaaccacaa cccgccgcgg
     5401 cgactgaaga cttcctgacg aactgtaccg tccattttcc acggataccc tttggtatgc
     5461 tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg tgcgcaccgg attaagcgaa
     5521 ccaaacagcg tctgccccat tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat
     5581 tgcttttctg tacgcaccga atctaaccgt tcgctataac ttttgccgtt tggcgatttg
     5641 atttttagcg cggtatgaac cagaaaagca ggaatgtgca tccgttcagc acgacggtca
     5701 atctcttgcc aggcgatctt gcttaagccc ggaacggccg gatctgcctg ataatttgac
     5761 tcctgctgcg ccaccgccag caccgagcag acgttttcca gcgtcggtgc cagcccctga
     5821 ctttcaaagg tgatggcgag atctttcgcc caggcatcgc ggtctttcac gctcgcaggc
     5881 atcttctgcc gcacgacgct cgctacatct atcgcttttt cgcccttttt caacggttgc
     5941 ggagcctgac tactacaccc cgctaaaacc agaaccgcca acaatgacag cgaagataga
     6001 tgattgacgc gcgacattac ttctcctttt tagctcaagg tatgggttac ttcctgaatt
     6061 ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt tgtagataga catcaactca
     6121 accagtgtgg tccatgagtt aatcaggtac tggaaagcac cgcgaacctg accaaaaacg
     6181 ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga cggaaacagc
     6241 aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat gttgaaatac
     6301 atatagtgaa aatagaggcg aaaatagttt ttccgtacgg cgctaaacag ctcgcgtacc
     6361 gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt acggtaggcg
     6421 gcctctactc gctggttttt aaactccagc cccggcagtt tgatccctac cactgccagc
     6481 catccggtcc ccatcagcga ccagacgata gcggcaatca ccagaccata cggaatgtgc
     6541 ccgataatcg gcagctccgg cacatgcgcg gagagcgtta ccagcaccgg caggaaggcg
     6601 atcaacgtca tgatggcgtt gataaaactg acgcccatat tctccagcgt tgaagcaaaa
     6661 cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga tatgacgcag ttgttgccag
     6721 ttcgccatgt aatattcgtt catcgccgta cgccagcgga acacatagtg actgacaaag
     6781 aagttgttca gtacactgat caccaccgcg atcagcgcaa tccccagaaa gacgcccact
     6841 tcgcggtaaa attgttcgat agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga
     6901 tcatagaacg gtgcatacca ggcgttgacg gcgaccccga cttccaccaa aaaccaggtg
     6961 acgaagatga tcagtgcagt accgagtatt gaccagtatt gccaacggtg cgggctgtag
     7021 ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt agtaagcgta aaaaatcagg
     7081 aaatccaacg accagaaacg cgcggcgcta atcgggatct gcccggaagc gccggtgata
     7141 cgcgccaccc agtctccccc accggcttgc cagaagataa cggcaatcaa tgcccaaaca
     7201 aaggccgaga gaaaaaacgc tcccggcttt gggaaaaaag acttaaacat atgcactcct
     7261 gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccac tacaaagcgt
     7321 cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc
     7381 ataagaattg tttcgacgga tttcacaaac caaagattgt ttacataagc ggattaacac
     7441 gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt
     7501 acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat gagtagtata
     7561 aatacgctca gttaccttca ttcaatctat ggacaccacc gttgaaacgt agtctgcttt
     7621 tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag ccgatcactg
     7681 aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt tacggcagcg
     7741 gtgcaacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt cgtagttatg
     7801 gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt
     7861 ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt gctcgaccag gggaccgtaa
     7921 agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgccg acctacaatg
     7981 ggacgcccat taccctagtg aatctggcaa cccataccag cgccctgccc cgtgaacagc
     8041 ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat
     8101 acctttctac ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac tctaaccttg
     8161 cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat acccagttgt
     8221 ttgaagagca gattacccgt ccattgggga tgaaagacac cacctacacc ccctcaccgg
     8281 atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat aacacgctgg
     8341 cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc tggatgcagc
     8401 agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg cagacgttga
     8461 tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc aaagcggatg
     8521 cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgggg attattcaga
     8581 agacaggcgg tggtggcggt ttcattacct atatggcgat gatcccgcag aagaatattg
     8641 gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg agcgatggca
     8701 tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc gcatcctgat
     8761 taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat ttcgatatag
     8821 ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat gcgtgtacgt
     8881 tgatggatat aattcatcac accaatacgt gatcgcaatt cttcattcca gtcaatcatt
     8941 gataataatg ttgcgcggat ttgttcgtag gaattatgcc caatgagctg cacgtcgcgt
     9001 aattccagaa ttctattttg ccaagctaac cagtaaaaag cgtctcgcca gagttgattt
     9061 tgttcaataa gcgtaatggt ttgtttggct ggtagatgat atcccgtaca atttccttct
     9121 gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc cataatataa
     9181 ggggcctggg taataccgat aagtacgttt tcttctctac gcagagagat aactccctct
     9241 aaaataacga aagtatcttc gttattgaca tcactggaaa atataacttg cttttcatta
     9301 ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc aaattcctga
     9361 agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt caaatccttg
     9421 acgggcatta tttagattaa attaccagta tttcttcgga gtgaagaata ttaccaggta
     9481 tatttaacac ccacgttcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta
     9541 gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt
     9601 accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta
     9661 gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctgatc ttcgctgtag
     9721 gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta agactgaccg
     9781 tcaactttca tgtcgttgct cagctggtag tcatcaccag actggaacag accagaaacg
     9841 ctgccgtaag gagtcacgta accagcatca cccagtttga agtcgtaacc ggctttcaag
     9901 ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt
     9961 gcagacaggt cgttgttgaa gtgagagtag ctcaagctac catcaacaaa gacgttgttc
    10021 gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatctac ctgaccagaa
    10081 cggtcattca tgtcaccttt agcgaagcct gcagccgcac cgacgatcca cttagcgttg
    10141 ttaccgtcaa ctttggtatc aacaccgacc atgatgccgt taacatcctg atcatagttg
    10201 atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc gccgccgtta
    10261 tcagccaggc catgacgaga gttggtcaga cgagtaccaa cggtgtcttg ttccaggttc
    10321 cagatattgg tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc ggtcagctcc
    10381 atctgttgca gaacaacggt gttaccgcgc tgttcagcct gataggtgta tgcacccagg
    10441 tcagctttgt tagcagcaga gaaggtcgcg ttggttttgg tatcatcgat atagatgagc
    10501 tgcttacctg cgtaatcagc aatcgcacca gcgccagtgg agttgtcgat acgaactttg
    10561 tagttaccgg ttgcagctac aacgttgtct gcagagctgt tgtcaacttc gtcgccattg
    10621 ttaatgtcgt tgttaccatt aacggtcaag tgaccatcag agttcatcgc aataacacca
    10681 taaccgtagt tagctttgct ggtgtcgttg gtacgatcgt tgacgaggtc tgcattcagg
    10741 atgtaatcat tactatgaat gtcaaacaca cctgcgtaaa cattaccgtt gttatcttta
    10801 acgttgccac tggtcagttc cagagtatca gttgcaaata cgctggagat gtcattcatg
    10861 ttggtgttta catccagcac accaccgtta gtaacggtaa tggtattggc atacagcggt
    10921 gcatatgccc aagctgaagc agtatcttca ccgaagttaa cggttgagaa ggtatcaatg
    10981 gtcaggtgat cggtgttaac ttcaccgtta acttccattg aagaaccacc gttcagagtg
    11041 atggtgtcag aagtcagaga agaatctgca ctcacgttaa cctgagaacc gctgttgatc
    11101 gccagggtat cgatcaggga agttttcgtg gtttcccaat cagaaccgtt gttcagaaca
    11161 acatcgaata cgccgctctg gaatgcgtta gcgttatcaa cacgattcca gccatcttca
    11221 gagtaagaag cgtacgggtt caggctgttt ggtgcaacgt cgtagaaggc ttcgtcagca
    11281 acaacaacgt cataatcaat attcgctgaa ccaacccatt tggtgttgtt cagagtcagg
    11341 ttcaggtgat cgacgttctg atcgtcagtt gcccaaccgc catgagtgta agcagtggta
    11401 gagttgtcaa tcaggtgacc cgtcgcgtca aagttagcgt tccaggtact gtcaaataca
    11461 acgtcaccaa gcaactgaga gttgttgaac gtaacgttgt tgaccatacg ataatcagaa
    11521 gtgtcatcat cgatgaaggc taacgcaaca tcatcagcag gagatgcgcc attgccgtag
    11581 tcacttggtt caacagaatt gccgaaatga ccacgatctg attgttcgtc ttcagtccat
    11641 gaaccagaag ttacagtaga gtcattaaca acgatgttgt tgtcccactg atgaccttcg
    11701 gtattgtagg tcgcattacc gaggtcgtgt tgaaccaaag taatacctgc aacatgggag
    11761 ttgttgctga tgttgatgtt actttcaaca tccagagtca cagcggtacc aagaccagcg
    11821 gttaccaaac gatcataatc tgtcttagat gttttaccgt ctgcatccag ataagtatcg
    11881 gtgaagtaga aggcgtcaaa atcatcgtca atagtagaat tagcgatgtt cagagtgaag
    11941 atgtcgccat caacccagtc gtggccagta taatcgctac cgttggtcca gacgtagtcg
    12001 ccataaccaa cctggtaaga tgtaaccata ccatgaacaa ttgagttgga gatgttcagt
    12061 gtattaccgg tatctgtgcc ataatcgtgg ttcagataat aagtagaaat tacaccattt
    12121 acagtgtcgc cgctgaaagt ggtatcaaag taaccatcag ctgcgtggtc atatacataa
    12181 ccaccgtaag tcagataatc gtgatcaatt ttagtgctgt caccccaatt gtagatggtg
    12241 gctgcgtcat cgtgataccc gaaggtgtta taagtagctg cattagcctg agaggtgata
    12301 gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca ggagtgcatt
    12361 tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc atagaaaaca
    12421 tattatgttt cgcgctaacg attatgctgc gttacagacg aatggttcaa ttattctttc
    12481 ccctaagtcc gaatgtggac atatataatt tatggagaaa aacacgcatc aaattgataa
    12541 aaggacgata tttcaacatg ttgcagataa tgttattttt gatacatacg cgttataaat
    12601 atcaaaaaca caaaccagat aaatatctta tcaatataca acaaagtttg aattattcaa
    12661 tttatagcag cacaaccaaa cgccaaaaaa aataataacc aagagctata aataaagatt
    12721 aaaaatattt tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct
    12781 ctctttcatt ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct
    12841 tcaggtatac tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc
    12901 aacgccctcg tcgcctacgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac
    12961 ttagccttaa cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag
    13021 ccgttgaagc catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg
    13081 aacgcatcgc caacgccggt atccgttccg taatgacttt tggcatctct caccataccg
    13141 atgaaaccgg cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct
    13201 gcaagcagac tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt
    13261 ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa
    13321 atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg
    13381 ccgcgatgga cggccaggta caggcgattc gtcaggcgct ggacgctgca ggctttaaag
    13441 atacggcgat tatgtcgtat tcgaccaagt tcgcctcttc cttttatggc ccgttccgtg
    13501 aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc
    13561 gtcgcgaggc gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg
    13621 ttaaacctgc tggagcgtac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc
    13681 cgattggcgc gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg
    13741 gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag cgtgcgggtg
    13801 cggatctgat tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat
    13861 ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc
    13921 ctaaccctct ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa
    13981 aacggtttat ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg
    14041 gcattggcat agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag
    14101 cgccaacgca cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct
    14161 tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc
    14221 gcctgtttac cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc
    14281 gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg
    14341 aacgatttac ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca
    14401 gagagcgcct cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt
    14461 aactctttag ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta
    14521 tgccagttgt cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca
    14581 accccttcgg catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc
    14641 gcgcgttgct gctgcggcgt aatagcttga tcgcgcagaa ataccacctg atagcgcagc
    14701 accgcatggt aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc
    14761 gaaatttgtg cgccgatata cggccccagc ggggtaatac tcagacgttc actcattgta
    14821 cttctccatg ccagggcgtc agacggcgct gtaacgcgcg cagacccagt tctaaaagaa
    14881 aggcgataat cgcaatcacc gcgatccccg ccagtaccac gtcagtcgcg agaaattcac
    14941 ccgctgactg aaccataaat cctaaaccgc gtgtcgcggc aatcagctcc gccgctacca
    15001 gcgtagacca gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc
    15061 cgggcaaaat gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac
    15121 gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcac cgacatcgcc accggtgcaa
    15181 aaatcgctaa atagatcaat aagatcttcg aggtttcacc aataccaaac cagatcacca
    15241 tcagcggcaa ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa
    15301 tgccgcgcac cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg
    15361 ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg
    15421 cgtccataaa tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg
    15481 gcggcaaaaa cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta
    15541 aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca
    15601 gccgccgcga atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc
    15661 aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct
    15721 gcggctcgac tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac
    15781 acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat
    15841 atcgtgggta atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt
    15901 ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag
    15961 ctggggattc gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag
    16021 ctgccagata tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc
    16081 gatttccagt cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc
    16141 ctgtacattg cgccacggta gtagcccttc attctgaaaa accaccccgc gctctgctcc
    16201 cggtccctca atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc
    16261 ggcaatcaga ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag
    16321 ctcgccgctt tccatcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc
    16381 atagagatga gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac
    16441 gtactggctg taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc
    16501 ggtgtcgatg attgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt
    16561 cagataggta ttccccttca ccagccccgg aacgtcacct tccggcacgc cgcttaaacg
    16621 cgccagtttg ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg
    16681 ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc
    16741 aaaatctttg cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc
    16801 agaatcggtc aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata
    16861 agcaccatca atatcacccc gctgccatgc ggcgataatc gcgggcggct gcaggttcac
    16921 aatctccact tgccctggtt taatccccca gtgtttcagc gccgccagca ggctgtagtg
    16981 ggtggtggag ataaacggta cggcaatgcg tttgccaatc aaatcttcgg gtttgctgat
    17041 agttttcttt accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc
    17101 aatcggcacc tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt tgccgatttg
    17161 cacgtcgcca gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc
    17221 cacgctcgcc ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc
    17281 ggctgaggtt tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatg
//
LOCUS       DAJTVE010000089        17295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000089 DAJTVE010000000
VERSION     DAJTVE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            93..1133
                     /locus_tag="OO439_002794"
     CDS             93..1133
                     /locus_tag="OO439_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6317119.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO439_002795"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO439_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6317120.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO439_002796"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO439_002796"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6317121.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="OO439_002797"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="OO439_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6317122.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO439_002798"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO439_002798"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6317123.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="OO439_002799"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="OO439_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6317124.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="OO439_002800"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="OO439_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO439_002801"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO439_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6317125.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO439_002802"
     CDS             complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO439_002802"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6317126.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO439_002803"
     CDS             complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO439_002803"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6317127.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12900..14081)
                     /locus_tag="OO439_002804"
     CDS             complement(12900..14081)
                     /locus_tag="OO439_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6317128.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14336..15565
                     /locus_tag="OO439_002805"
     CDS             14336..15565
                     /locus_tag="OO439_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6317129.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16025..16657
                     /locus_tag="OO439_002806"
     CDS             16025..16657
                     /locus_tag="OO439_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6317130.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16991..>17295
                     /locus_tag="OO439_002807"
     CDS             16991..>17295
                     /locus_tag="OO439_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6317131.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3976 g         4227 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10741 tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga gattattatt
    10801 aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata attgatatgg
    10861 attatttcat aaccatgata tgaattatga tgacctacag gtataaaaaa ccctgccatg
    10921 aggcagggtc ataaaagtaa gaggaatgaa ttaacgagcc agccaaccgc catctacggc
    10981 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat
    11041 caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg
    11101 ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc
    11161 attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cgcccatcac
    11221 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11281 cgtgatattg ataatcttgc cgccattgcg ttgcgcgata aagtgtttcg ccgctgcctg
    11341 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11401 gaactcgaga gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11461 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
    11521 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
    11581 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11641 ctggcccagt ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
    11701 acttaaaatc ataacaatac cttattcgtt tatgccgaca actagcgcaa atctttaacg
    11761 gccactatgc cgacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
    11821 cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg acgccggaat
    11881 ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
    11941 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
    12001 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
    12061 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
    12121 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
    12181 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
    12241 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
    12301 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
    12361 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
    12421 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
    12481 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
    12541 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
    12601 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
    12661 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
    12721 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
    12781 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
    12841 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
    12901 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
    12961 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
    13021 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
    13081 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
    13141 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
    13201 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
    13261 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
    13321 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
    13381 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
    13441 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
    13501 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
    13561 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
    13621 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
    13681 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
    13741 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
    13801 gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
    13861 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
    13921 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
    13981 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
    14041 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
    14101 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
    14161 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
    14221 gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat acttaacctg
    14281 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
    14341 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
    14401 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
    14461 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
    14521 cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac actactatgg
    14581 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
    14641 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
    14701 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
    14761 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    14821 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
    14881 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    14941 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    15001 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    15061 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    15121 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
    15181 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    15241 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
    15301 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    15361 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
    15421 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
    15481 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
    15541 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
    15601 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
    15661 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
    15721 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
    15781 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
    15841 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat
    15901 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga
    15961 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
    16021 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
    16081 ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat
    16141 gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa
    16201 ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct
    16261 attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt
    16321 cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc
    16381 tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt
    16441 tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat
    16501 aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga
    16561 tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa
    16621 cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca
    16681 tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc
    16741 aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
    16801 atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt aaagttttac
    16861 gttgcaacat taaagcctca tttcaatcat catgataaat ataaaattaa tatatattta
    16921 tgccgtaaat accaatatac ttagcaaact atgtgatctc catttcgatt gatttagtgt
    16981 ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg agcaccgaaa
    17041 attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag ccagtcgatg
    17101 tttatcactt ctacttgaag cttttcctga tgtggttgca caacaagatt ttttcaccag
    17161 agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga acatatccat
    17221 tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgctaattg caaccgtgcc
    17281 gagagaggat tcaag
//
LOCUS       DAJTVE010000090        17144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000090 DAJTVE010000000
VERSION     DAJTVE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO439_002808"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO439_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6317132.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO439_002809"
     CDS             2219..2767
                     /locus_tag="OO439_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6317133.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO439_002810"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO439_002810"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6317134.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO439_002811"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO439_002811"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6317135.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO439_002812"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO439_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6317136.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO439_002813"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO439_002813"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6317137.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO439_002814"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO439_002814"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6317138.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OO439_002815"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OO439_002815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6317139.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO439_002816"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO439_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6317140.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO439_002817"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO439_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6317141.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO439_002818"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO439_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO439_002819"
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO439_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6317142.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO439_002820"
     CDS             complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO439_002820"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6317143.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4298 a         4421 c         4351 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggtgtagcg actatcagtc
    16621 catcagcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg aaacgagcgt
    16681 aaagcagatc ttccggggcg aagttttgca gattccagtg ataaacctct acatccaggc
    16741 catttagttt ttcccgcgca tattccagca aggagctgga gcgagaagga aagcgaggac
    16801 tacccgccag ggtgatgaca cgcatactct ctccttataa ccaattgttc tttttttgtt
    16861 aacattgata acaattcggt cagtctgtcg gagagacaag aaaatgccaa atataaattt
    16921 tgtgtatctt tttctgaaaa taaactaaag aaagggcttt atatatcacg catatttatt
    16981 tgttggatag tcattagatg ttgaatcaat gaattactcg ttcagttaat atttaaatat
    17041 tcatgaaatc tataaattaa agatttgtca cttattggat ttagtatgct tgatttaaat
    17101 ggcggtcaat tattgttatt attaagtcta atgtcattta aagg
//
LOCUS       DAJTVE010000091        16495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000091 DAJTVE010000000
VERSION     DAJTVE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..2496)
                     /gene="rnr"
                     /locus_tag="OO439_002821"
     CDS             complement(55..2496)
                     /gene="rnr"
                     /locus_tag="OO439_002821"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6317144.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2535..2960)
                     /gene="nsrR"
                     /locus_tag="OO439_002822"
     CDS             complement(2535..2960)
                     /gene="nsrR"
                     /locus_tag="OO439_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6317145.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3165..4463)
                     /gene="purA"
                     /locus_tag="OO439_002823"
     CDS             complement(3165..4463)
                     /gene="purA"
                     /locus_tag="OO439_002823"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6317146.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4567..4764)
                     /locus_tag="OO439_002824"
     CDS             complement(4567..4764)
                     /locus_tag="OO439_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6317147.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4846..5850)
                     /gene="hflC"
                     /locus_tag="OO439_002825"
     CDS             complement(4846..5850)
                     /gene="hflC"
                     /locus_tag="OO439_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6317148.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5853..7112)
                     /gene="hflK"
                     /locus_tag="OO439_002826"
     CDS             complement(5853..7112)
                     /gene="hflK"
                     /locus_tag="OO439_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6317149.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7198..8478)
                     /gene="hflX"
                     /locus_tag="OO439_002827"
     CDS             complement(7198..8478)
                     /gene="hflX"
                     /locus_tag="OO439_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6317150.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8555..8863)
                     /gene="hfq"
                     /locus_tag="OO439_002828"
     CDS             complement(8555..8863)
                     /gene="hfq"
                     /locus_tag="OO439_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6317151.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8949..9899)
                     /gene="miaA"
                     /locus_tag="OO439_002829"
     CDS             complement(8949..9899)
                     /gene="miaA"
                     /locus_tag="OO439_002829"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6317152.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9892..11739)
                     /gene="mutL"
                     /locus_tag="OO439_002830"
     CDS             complement(9892..11739)
                     /gene="mutL"
                     /locus_tag="OO439_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6317153.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11749..13086)
                     /gene="amiB"
                     /locus_tag="OO439_002831"
     CDS             complement(11749..13086)
                     /gene="amiB"
                     /locus_tag="OO439_002831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6317154.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13105..13566)
                     /gene="tsaE"
                     /locus_tag="OO439_002832"
     CDS             complement(13105..13566)
                     /gene="tsaE"
                     /locus_tag="OO439_002832"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6317155.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13538..15085)
                     /gene="nnr"
                     /locus_tag="OO439_002833"
     CDS             complement(13538..15085)
                     /gene="nnr"
                     /locus_tag="OO439_002833"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6317156.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15084..16223
                     /gene="queG"
                     /locus_tag="OO439_002834"
     CDS             15084..16223
                     /gene="queG"
                     /locus_tag="OO439_002834"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6317157.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16206..16259)
                     /locus_tag="OO439_002835"
     CDS             complement(16206..16259)
                     /locus_tag="OO439_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317158.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3738 a         4715 c         4140 g         3902 t
ORIGIN      
        1 aacaaagcgc attttgtcag caatctaacc ctcttctttt aaagagggta ttgatcactc
       61 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcggctattt tctgcgtttt
      121 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt
      181 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacgacgctt
      241 gccgcctttt ttgcctgcat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
      301 gcgcggtgcg cgttcactgg agatcagact aaagtcgatt ttgcgctcgt ccatattaac
      361 cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc tggattcccc
      421 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
      481 gaccagacca tcaatgaaca agtcgtccag acggacgaag aagccaaagc cagtgacgct
      541 ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac acttcagcca
      601 gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
      661 cagttgcaac atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttgccctg
      721 atgcccctgc tctttcgcca gcagatactt aatggcgcgg tgcagcgtca ggtcaggata
      781 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccaggccaa agtgaccacg
      841 gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc agcatggttt gcagcatttc
      901 tgcatcagga cggtcggcaa ccgactccag cagttccgcg tagtcacgcg gttccggctt
      961 gttaccaccc ggcagctcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
     1021 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
     1081 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaatttgt gcgcgtcgtt
     1141 acgctgggtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
     1201 ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca ctttatagag
     1261 gttatgcaac tcttcgagat gcttaaccag cgggacgtac tgctcgcgca gatcctgatc
     1321 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
     1381 ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
     1441 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
     1501 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
     1561 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
     1621 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
     1681 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     1741 cacttcttct ttcagccccg caacctgttg ctcaacagcc tgcggccaga tatacggaat
     1801 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt cgcccagcac
     1861 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
     1921 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     1981 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     2041 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     2101 gtcagcaccc agcggctgcg ccagcacctg atcgccatga atacaggttt tcatctgctc
     2161 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     2221 gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     2281 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     2341 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     2401 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     2461 ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     2521 gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt ctcttcaacc
     2581 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     2641 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     2701 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     2761 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     2821 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     2881 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     2941 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     3001 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     3061 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     3121 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacgga
     3181 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
     3241 ccggtcagct cttcaatacg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     3301 tctttcacgc cgaaagtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     3361 cctttccagt cgtcagctgc cagcggagtg gtagccactt cgcgaccatc cggcatacgg
     3421 taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagctt ggtcaggcaa
     3481 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     3541 gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca gaggaactcg
     3601 ccagtttcat caaacagttc ggtcgggaac ggacctgcac ctacacgagt tgagtaagct
     3661 ttgaggatac ccagaacata atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     3721 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     3781 agcgtaccct gcgcaccttc aaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     3841 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     3901 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     3961 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     4021 cgcagaccgc gacgtgctac tttatcttca taagcaggcc cgataccacg accggtggtg
     4081 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac gtgataatca
     4141 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     4201 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     4261 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     4321 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     4381 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     4441 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     4501 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga tcagtctgtt
     4561 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     4621 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     4681 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     4741 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     4801 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     4861 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     4921 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     4981 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
     5041 gatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     5101 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     5161 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     5221 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     5281 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     5341 cgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     5401 ttcgagggtc agacgaccac gggaatcggt gacgatgtct ttcacgtcca ggcgaccaat
     5461 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     5521 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     5581 atcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     5641 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     5701 cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     5761 aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     5821 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     5881 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     5941 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     6001 ttatcgctct tcgccgcagg ggcgttaccg cctttcagca tctggtctaa cggcagaacc
     6061 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     6121 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     6181 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     6241 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
     6301 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     6361 aacgccgctt ttacttcttc cggcggacga gctgcctgga agttgacgtc cagcagagtg
     6421 atacccatgt catacggacg gatagtctct tccagttcgc gctgagtatc gctacgaatc
     6481 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     6541 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     6601 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     6661 gtcagcatca caccagaagc ggccagttca cgcacggctt ccacgttcac cggtttgact
     6721 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccagatggct gaatttacca
     6781 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     6841 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     6901 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     6961 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     7021 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     7081 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     7141 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     7201 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     7261 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     7321 aagctgataa aaacgacttc tcagacgtcc ttcctgcggc ggcaaacgca atgtatgctg
     7381 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     7441 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     7501 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     7561 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gtacatccgc
     7621 cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     7681 tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     7741 ttcaccgaca tccgcgacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     7801 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     7861 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     7921 ctcacgctgc ttttcaactc tttctaggcg cgactgtatc tgcacgatgc gattacgcaa
     7981 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
     8041 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
     8101 tagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
     8161 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     8221 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga cagcttccgc
     8281 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     8341 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     8401 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     8461 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     8521 ctggctcccc gtgtaaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt
     8581 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
     8641 cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt
     8701 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
     8761 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
     8821 gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata
     8881 tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt
     8941 gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct
     9001 ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc
     9061 ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg
     9121 ccttcaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta
     9181 tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc
     9241 tgatgaaaac gctgctcaat gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg
     9301 aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt
     9361 ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga
     9421 atccttgctg ccgcaaccgg atctacctcc tgaagttgac gatgcaatga ctcccaacct
     9481 tgctctgccg cctgttgctc aattctggcc cgcacttccg ggtctgccga cggtagcggc
     9541 gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc
     9601 cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc
     9661 gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct
     9721 tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg
     9781 cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag
     9841 gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc
     9901 tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt
     9961 tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat
    10021 tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg
    10081 ttcgaatacg gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt
    10141 ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa
    10201 atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt tttcttcgcc
    10261 agaaactttt agccgcaacg gaataagcag cggctgagcg caaactggcg cttcacccgg
    10321 cgtcagttgt acctgacgca gccaacgttc tgccactggc aaggctaaaa gtgaaatgtt
    10381 gccgtcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact
    10441 ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttaatt tttgcatcgg
    10501 cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt agcctggctg
    10561 cgcattcggc cagggggcag ccggacgact gcctgatgct ggcgcaggag tgtagcgcgg
    10621 agctaccggc tcacgagctg ccggttctgc aaagtgattg cgccccgccg ccacgcggtt
    10681 ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc agcggtagcg gcgtttccag
    10741 ttgctgttgt agcacgctca gcacgccctg atagataaaa tcatgcacca gacgcgactg
    10801 atggaaacgc acttcgtgtt tggcggggtg cacgttgacg tccacctgat gcggatcgat
    10861 ctccagatac agcacaaatg ccggttgctg atcggccccc agtttgtctt cgcaggcctg
    10921 gcggatcgcg tgattgatca ggcgatcgcg catcatgcga ccgttcacgt agcaatactg
    10981 aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc acccagccgc gtagcgtgag
    11041 atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga aaagcggtgc cgcaaatcgc
    11101 gcctaagcgc cgttcttttt gcccgccttc cggcactgcg cggtactgac gcacaatttt
    11161 accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat
    11221 ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt
    11281 gttgtagaac agatccagca cctccagcgt cgtccccaca ggatgcgccg ccggttttac
    11341 cgtcacgttc atatcgcgcc cttcggcata ggcctgccag gcttcctgct gttctgcggt
    11401 gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa
    11461 gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg
    11521 agccagcgcc agcgccagct catctttttt gataccgcag ccgttatcac gaatgcggat
    11581 aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct
    11641 gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat
    11701 ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag
    11761 cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc gttgccccct gcggcgcaga
    11821 ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt ctgccagctg
    11881 ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt
    11941 ttcgaccagt actgacggga tatccggcga acgcagaacg ccgagactcg cgtgttctgg
    12001 tcggcgttta tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc
    12061 ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg
    12121 gtcagactga ctgttcgcca gcacatcgcc cgccccaccc agcagctccg actgtttctc
    12181 gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga gcacccatac
    12241 ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt
    12301 ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc gagataaagt aatccccgtc
    12361 acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtgcgca atttacgcgc
    12421 gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg
    12481 accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg
    12541 cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt
    12601 tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac
    12661 gcgtttcgca accacgggcg gtggaggagg cggtggcggc acatcggcgt taatcgtaaa
    12721 gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac cgttttcggt
    12781 aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccagagc gaatcgcctt
    12841 caccagatta ttgccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat
    12901 atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa
    12961 actcaacgtt atccgcgcct gttgattacc gttagaaacc tgaatatcag agagcgtcgc
    13021 ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc aaccaatttc tgatgcgata
    13081 catcatcccg ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc
    13141 agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc
    13201 gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc
    13261 atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg
    13321 gtacaaatca aagtgataaa ccattaaatt gtcgagcgta tagggttcga ccagcgtata
    13381 agtggggctt ttgacattac cctgatgacc cagagcttgt aaaaaacccc ggctaaaggt
    13441 ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt
    13501 cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa ttactcgatt
    13561 catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat
    13621 cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt
    13681 gcgcgacaca gcccgcacag gcggcatcat acggcgacag tttttgccca agcaatgcgc
    13741 caataatacc agagagcaca tcgcccatgc cgccgctcgc catgcctgca tttccggcat
    13801 caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg
    13861 ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt
    13921 cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat
    13981 tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg
    14041 gtttgcgaaa attctcaact ttttgcagtg cttttttccc ccactcttgc tggcccagac
    14101 cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatcgtca
    14161 gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg cgcaatgttt tcactgcggg
    14221 tcagtactcg gactaaacca gcaccagcac gcagcgccgc ttcccccgtc atacgaatag
    14281 cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg
    14341 aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt
    14401 tcgtctcctg acctgccagc cagctatcca gccccagtga gtcaaaatgc agttgtcctg
    14461 taacatcccg cgcttttcca gtaagcaagc ctggtttcag cgcaataaaa gtgatggtgt
    14521 gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga
    14581 tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg
    14641 attcgcgggg cgcttgctgc aagccggtac cgagcagcgc atcaacaatc agatctaccg
    14701 attcgggcca gacaatattc gaagcatgga tctcgccacc cgcgtttaac catgcttcgc
    14761 gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct
    14821 caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat
    14881 gaccgcacag caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg
    14941 cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcaca tctgccgcct
    15001 cgcgttctcc gcggcggata tcgtcggcgt accagacggt gtgtggtata cttacggggt
    15061 ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa
    15121 aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc
    15181 tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg
    15241 actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttatta ccaggtacgc
    15301 tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc
    15361 tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac
    15421 ttctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga
    15481 attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg
    15541 ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt tctttcttct
    15601 ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat
    15661 gcggtaaatg catagcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata
    15721 ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga acttgaaggg gcgatcccgg
    15781 aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct
    15841 gcccgtggaa tcgctattcg caactcacta cagaagacga tttcagcccg cgtaagccgc
    15901 tacacgcaca ggaactcatt gagttatttg cctggagcga agagaagttt ttaaaagtca
    15961 cggaaggatc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg
    16021 cattaggcaa tgcgccctgg gatgaaacga ttctggcggc gctggagagt cgcaaaggtg
    16081 agcacccact tcttgatgag cacatagcgt gggcgattgc gcagcaaatc gagagacgaa
    16141 atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa
    16201 aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt
    16261 gcaattcaag acgcataaaa tcgtcaagtg atcgactcga caaaacggaa tcttgatttt
    16321 taacttattt atcaataggt taaaaaaata ccattcaccg tgcgaagttt ctttgaaatt
    16381 aagattacag cctgaggatg tggataactc tgtttacaag agtttgtcag agacaacaaa
    16441 aaccagcaat aacgcatcgc gtcgctgtgg atattttatt gagagaagaa tttgg
//
LOCUS       DAJTVE010000092        16147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000092 DAJTVE010000000
VERSION     DAJTVE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..384
                     /locus_tag="OO439_002836"
     CDS             <1..384
                     /locus_tag="OO439_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6317159.1"
                     /translation="ICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNVEQSWIP
                     VVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKESYSSGGF
                     TTKKASTVSRGGYGRSSSARGHWGG"
     gene            387..1550
                     /locus_tag="OO439_002837"
     CDS             387..1550
                     /locus_tag="OO439_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6317160.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1634..3259
                     /locus_tag="OO439_002838"
     CDS             1634..3259
                     /locus_tag="OO439_002838"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6317161.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3376..3651)
                     /gene="yjfN"
                     /locus_tag="OO439_002839"
     CDS             complement(3376..3651)
                     /gene="yjfN"
                     /locus_tag="OO439_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6317162.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3800..4129)
                     /gene="bsmA"
                     /locus_tag="OO439_002840"
     CDS             complement(3800..4129)
                     /gene="bsmA"
                     /locus_tag="OO439_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6317163.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4311..5060
                     /gene="yjfP"
                     /locus_tag="OO439_002841"
     CDS             4311..5060
                     /gene="yjfP"
                     /locus_tag="OO439_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6317164.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5057..5812)
                     /gene="ulaR"
                     /locus_tag="OO439_002842"
     CDS             complement(5057..5812)
                     /gene="ulaR"
                     /locus_tag="OO439_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6317165.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(5920..6984)
                     /gene="ulaG"
                     /locus_tag="OO439_002843"
     CDS             complement(5920..6984)
                     /gene="ulaG"
                     /locus_tag="OO439_002843"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6317166.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7339..8737
                     /gene="ulaA"
                     /locus_tag="OO439_002844"
                     /pseudo
     CDS             7339..8737
                     /gene="ulaA"
                     /locus_tag="OO439_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8753..9058
                     /gene="ulaB"
                     /locus_tag="OO439_002845"
     CDS             8753..9058
                     /gene="ulaB"
                     /locus_tag="OO439_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6317167.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9068..9532
                     /gene="ulaC"
                     /locus_tag="OO439_002846"
     CDS             9068..9532
                     /gene="ulaC"
                     /locus_tag="OO439_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6317168.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9546..10196
                     /gene="ulaD"
                     /locus_tag="OO439_002847"
     CDS             9546..10196
                     /gene="ulaD"
                     /locus_tag="OO439_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6317169.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10206..11060
                     /gene="ulaE"
                     /locus_tag="OO439_002848"
     CDS             10206..11060
                     /gene="ulaE"
                     /locus_tag="OO439_002848"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6317170.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11060..11746
                     /gene="ulaF"
                     /locus_tag="OO439_002849"
     CDS             11060..11746
                     /gene="ulaF"
                     /locus_tag="OO439_002849"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6317171.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            11843..12394
                     /locus_tag="OO439_002850"
     CDS             11843..12394
                     /locus_tag="OO439_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6317172.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12469..12784)
                     /locus_tag="OO439_002851"
                     /pseudo
     CDS             complement(12469..12784)
                     /locus_tag="OO439_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13111..13506
                     /gene="rpsF"
                     /locus_tag="OO439_002852"
     CDS             13111..13506
                     /gene="rpsF"
                     /locus_tag="OO439_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6317173.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13513..13827
                     /gene="priB"
                     /locus_tag="OO439_002853"
     CDS             13513..13827
                     /gene="priB"
                     /locus_tag="OO439_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6317174.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13832..14059
                     /gene="rpsR"
                     /locus_tag="OO439_002854"
     CDS             13832..14059
                     /gene="rpsR"
                     /locus_tag="OO439_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6317175.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14101..14550
                     /gene="rplI"
                     /locus_tag="OO439_002855"
     CDS             14101..14550
                     /gene="rplI"
                     /locus_tag="OO439_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6317176.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14662..15633)
                     /locus_tag="OO439_002856"
     CDS             complement(14662..15633)
                     /locus_tag="OO439_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6317177.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15686..16102)
                     /locus_tag="OO439_002857"
                     /pseudo
     CDS             complement(15686..16102)
                     /locus_tag="OO439_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3789 a         3948 c         4288 g         4122 t
ORIGIN      
        1 atttgcgcgc gtggctggaa caacgccaaa acggcatttt atgccgatgt tccgaagaat
       61 atgactcagc agaactgtca gtctaagtac gaaaattcct actacgacaa tgttgaacag
      121 agttggatcc cggtcgtttc tggatttttg ttaagccggg ttattcgtaa agatcgcgat
      181 gagccgttcg tctataacag cggcggatcc tcatttgctt cccgccccgt ctggcgcaac
      241 acttccggtg attactcctg gcgctccggt tctggcaaaa aagagtctta ctcttcaggc
      301 ggctttacca ccaaaaaagc gtcaaccgtt tcgcgcggcg gctatggtcg ttcttccagc
      361 gcccgtgggc attggggagg ctaatcatgc tgagacacaa cgttcctgtg cggcgggatc
      421 tggaccaaat tgccgctgac aacggtttcg actttcatat catcgacaat gaaatctatt
      481 gggacgagag tcgggcttac cgttttactc tgcgccagat tgaagagcag atcgaaaaac
      541 caactgcgga actgcatcag atgtgccttg aggtggtgga tcgcgcggtt aaagacgaag
      601 agatcctgac gcaactggcg atcccgccgt tgtactggga tgtgatcgcc gaaagctggc
      661 acgccagcga tccttcgctg tatggccgga tggattttgc ctggtgtggc aatgcgccgg
      721 tgaagctgct ggagtacaac gccgatacgc cgacttcgtt gtatgagtcg gcttatttcc
      781 agtggttgtg gctggaggat gcccggcgca gcggcattat tccgcgtgat gccgatcagt
      841 acaatgccat tcaggaacgc ctgatttcac gctttagcga gctttatagc cgggagccgt
      901 tttatttctg ctgctgtcag gacaccgatg aagacaggac taccgtgctg tacttgcagg
      961 actgcgccca gcaggcaggg caggagtcgc ggtttatcta cattgaagat ctcggtttgg
     1021 gcgtcggcgg tgtactgacc gatcttgatg ataatgtcat ccagcgtgca tttaagctgt
     1081 atccgctgga gtggatgatg cgtgacgata acggtccgct gctgtgcaag cgtcgcgagc
     1141 aatgggtgga gccgttatgg aaaagtatct tgagtaataa agggctaatg ccgctgcttt
     1201 ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg gtttgagggt gaaaaatcgc
     1261 agattgccgc tggcgaaagc tatgtgcgta aaccgatcta ctcgcgcgaa ggcggaaacg
     1321 tcaccatttt cgacggtcag aataacgtcg ttgaccacgc tgatggtgat tacgccgatg
     1381 aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt tggcgatagc tacacactca
     1441 tcgatagctg gattgtcgat gatgaagcgt gcggaatggg gatccgtgaa gataacacac
     1501 tgatcaccaa agacacctca cgtttcgttc cgcattacat tgctggataa gaatgtttta
     1561 gcaatctctt tctgtcatga atccatggca gtggccatac taatggtgac tgccattgat
     1621 ggagggagac acagtgcact ggcaaactca caccgttttt aatcaaccta taccattaaa
     1681 taacagcaac ttatacctgt ctgatggcgc gctttgcgaa gcggtaacgc gtgaaggtgc
     1741 tggctgggat agcgattttc tagccagtat tggtcagcag ttaggaacgg ctgaatccct
     1801 tgaactgggg cggctggcga atgtaaaccc gcctgaactg ttgcgctacg atgcccaggg
     1861 gcggcgtctg gacgatgttc gtttccatcc cgcatggcac ctgctgatgc aggtgttatg
     1921 taccaatcgg gtgcacaatc ttgcctggga agaagacgct cgctccggcg catttgtggc
     1981 gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct gggtcgttat gtccaataac
     2041 gatgaccttt gccgccacgc cattgttgtt acagatgtta cccacgccgt ttcaggactg
     2101 gaccacgccg ctgctgagcg atcgctacga ttctcactta ttgccaggtg ggcaaaaacg
     2161 cggtttgttg attggcatgg gaatgacgga aaagcagggt ggttccgatg tcatgagcaa
     2221 caccacccgt gcagagcgtt tggaagatgg ctcttatcgg ctggtggggc ataaatggtt
     2281 tttctcggtt ccgcaaagcg atgcgcatct ggtgctggcg cagaccacgg gcggtctgtc
     2341 ctgttttttt gtgccgcgct ttttgcctga cgggcaacgc aacgcgattc gcctcgaacg
     2401 gctgaaagat aagctgggta atcgctctaa cgccagttgt gaagtggagt ttcaggatgc
     2461 cattggctgg ttattggggc aggaagggga aggaattcgt ctgatcctga aaatgggggg
     2521 gatgacgcgt tttgattgcg ccctgggtag ccatgccatg atgcgccgtg cgttttcgct
     2581 ggcgatttat catgcacatc aacgccatgt ttttggtaat ccattgatcc aacagcccct
     2641 tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa gggcaaacgg cgttgctgtt
     2701 tcgtcttgcg cgagcgtggg accggcgcgc cgatgccaaa gaagccctgt gggcgcgttt
     2761 atttacgcct gcggcaaaat ttgtgatctg caaacgaggt atgccgtttg tggccgaagc
     2821 gatggaggtg ctgggcggca ttggttattg cgaggagagc gagctgccgc ggctttaccg
     2881 ggagatgccg gtaaacagta tttgggaagg ttccggcaat attatgtgcc tggatgtgtt
     2941 gcgcgttctc aataagcaag cgggcgtata cgacttattg tcggaagcat ttgtggaagt
     3001 gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt ttacagcagc agctgcgtaa
     3061 gccagctgaa gaactggggc gagagattac tcatcagcta ttcctgctgg gctgcggtgc
     3121 gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg tggtgtcagg tgatgttaga
     3181 tacgcgcggc ggcgtacggt tgtcagagca gatccagaat gatttattgc tgcgggcgac
     3241 ggggggagtg tgtttgtaaa cgtatacgac tgatgcgacg ctggtttcga ttaactcaat
     3301 gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc cgcatccggc attgttcatt
     3361 tgctgtagat gacactcatg catacagtat cgcctgtacg cgccagttat caggctgctg
     3421 gtcttcgtac atggtaataa tgcggtacca tgacgcacct tgttcatcgg cctttaacgc
     3481 cacgatacgt tccacgtcct gcggatcccc tgaaatattg ctaactgaga tctgacctat
     3541 ttcattcagg cccgtcacgc agtcagcact ggcgaattct gcagactgtg ccgtggcgct
     3601 cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt aattgtttca tcgtcagctc
     3661 catttttcgt ttccccacat gttgcagggc attccatcgc ggtaataaac gcccaggctt
     3721 ccagccggta atttattgtg cacaggtaaa cgcataggga cgcaaagcaa tgtaaagctg
     3781 actatgtaag ttgtctgatt tatttacggt acaaaatggc ttgtgaatac cactgtcctg
     3841 tcacgatggt ttcgtccacc atgacgacaa cgtaataatc agcttttgca gcgacagctt
     3901 ttgctctgat ttctgctaat gcgtcatccg gagaaccccg aaccatcgtg cttacgctac
     3961 ctattcgctg taacccttgc gtctggtcgc ggcgaatctc ttgcggatgg tccgttactg
     4021 gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc acttaacatc aacaccaata
     4081 ataaactggc aaaccggtaa ataacgctat tacgtttcct gctaaccata gtgtagtgcc
     4141 ttattaattt aactttgggg gaatgttccc gaattgttac ctggtacgcg attttcgaat
     4201 gaaaacgtga cgaaagcgta acccacatct caacattatg aactaatacc gccagcagaa
     4261 acttgtgcta aggatttctt catgtcatac cagacaaaaa ggagagacag atgattgaaa
     4321 tagaatcacg cgagctggca gatattcccg ttcttcatgc ttatcctgtc gggcaaaaag
     4381 ataccccgtt accgtgcgta attttttatc acggctttac ttcatccagc ctggtgtata
     4441 gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt gatcatgccg gatgcgcccg
     4501 atcacggtag ccgttttagt ggcgacgaag cgcggcggtt aaatcaattc tggcaaatct
     4561 tgctacaaag tatgcaggaa ttcactactt tacgtgcggc aatagccgaa gaaaactggc
     4621 tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg cgcgatgacg gcactgggaa
     4681 ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat gatgggatcg ggctatttta
     4741 catcgctcgc ccgttcactg tttccaccgc tgatacctga aacggcagca cagcagaatg
     4801 aattcaataa cattatcgcg ccgctggcag agtgggaagc gacaaaccac ctggaacaac
     4861 ttggggacag acctctactg ctgtggcatg gcctcgacga tgacgttgtg cctgccgacg
     4921 aatcactacg tttgcagcag gccttaagcg agacgggacg ggataaactg ctaacctgtt
     4981 catggcagcc aggcgtgcgt caccgcatta cgcctgaggc gttagatgct gccgtgacat
     5041 ttttccgcca gcatctttaa acacgcagaa tgctgacccc ttgcgcttcc agttgttgca
     5101 ggatttccgg gttagcattt ttgccggtga taagcatatc gatttgatcg gcacggctaa
     5161 aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac cagtaccacc agtttcccta
     5221 ccacgctcag catcttctgc tctgccattg ctgtcagcat atcggtttta tacaaccctt
     5281 ctgcggtcag cccttttccg ctggtaaaca tccagtgccc ggcatagaga ctgttttcgc
     5341 tgccctgcgg gcttaaagtg atggactgac ttttgttgta ctgtccaccc ataatgatca
     5401 cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg tagataatta gtgatgattt
     5461 gcactggctt gccacacatt tcccgcccaa gcagaaacgc ggtggagccg cagttgatga
     5521 ctacgctttc gcccggatta accagctgcg aggccgcttt agcaatacgt actttttcat
     5581 cgtgattctg cgcctgatgc agattcatcg gcgtccagcg cgggcgctgt tgggtaatag
     5641 cttctgcgcc attgcgcacc tttttcagtt tgccgcgttc gtcaagttta ttgatatcgc
     5701 gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac tttctcaacg gtcacaaagc
     5761 ccaattgtgc gagcatttcc aggaggattt gatgtctttg tgcttcagtc atgagctatt
     5821 ccgataaaat ttgatttttt tagatgatat ttgaaatagc taggaaatac tacgccggat
     5881 agcgcgaact tctccacgct atccggtata aacgacagat tacaggaatg acttgaacgg
     5941 cagatccggt tcaatagtga agcaatcatc gaaaccgcgc ggatagtggt actcgaagtt
     6001 gtctttatcc agcggccagg taaatttgcc gccaacctgc cagataaacg gcttgaagcc
     6061 atacttcaga cgatcttttt tcatctccca cagcacgcgg atctcttgcg gatcggcctg
     6121 gaagtttgac cagatgtcgt ggtggaacgg gataaccact ttcgcattca gggcttcacc
     6181 catacgcagc atatcggcgc tggtcatttt gtcggtaata ccgcgcgggt tctcaccata
     6241 cgatcctaac gccacatcga tctgatgttc gttaccgtgc ttcgcataat agttagagta
     6301 gtgggaatcg ccactgtgat atagggaacc gccaggggtt ttgaacaggt agtttactgc
     6361 gcgatcgtcc atgccatctg gcagtacgcc agccgctttt tgatcggcag gcagggtgat
     6421 cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca atgtctttca cttttacaac
     6481 atcgcccggt ttgaccacga tgcaacgctc tttcggtacg ccccagccaa tccacaaatc
     6541 aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca cagttctgca tcacggcagc
     6601 agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc gccagtaccg cgtcgatctg
     6661 gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt aggtttggct gcagtttttt
     6721 cacgccagcc atgcgctgca tctggtgacc ctgtttcatt aacgggttac cgtgactttg
     6781 tttgccagtg ccgcaccaga aatcaacgca aacgttggtg ccaccttctg atttcaacca
     6841 gatcccggtg cagcctagcc accacatcgc aaatgtgcca ggggcgacct gctcttgttc
     6901 aatttcttca ttcagccagc taccccactc cgggaaagtg ctcaggatcc aggattctcg
     6961 ggtgatactt ttcactttac tcatcgccat ttaccttcat gatagttcaa ttcgaatcaa
     7021 tatgtgattg gttttgatta atcctgacac tattttttca ggaaggcaat gaccattttt
     7081 tgactttttg ccagggaagt tgttgttgat tttgagtgtg gaaagattta atggaatgtg
     7141 tagttaaatt aactaaatga atttaaataa ataattgttt cgttgtgtga atcccactct
     7201 atccgtgtgg aattatttgc gggtcgcgtc acatttaatc ataaataatc ttgttgtgat
     7261 tacttttgaa aattagagtg agtgcacaac attccgggtg tgcggaatac ccggttacct
     7321 cttcttcagg agatcgttat ggagatcctc tacaacatct ttaccgtgtt ttttaaccag
     7381 gtcatgacca atgccccgtt gttgctgggt attgtgacct gtctgggcta catcctactg
     7441 cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa ccataattgg tttcatgttg
     7501 ttgcaggcag ggtccggcat cctcaccagc accttcaaac cggtggtggc gaaaatgtcc
     7561 gaagtctacg gcattaaacg gcgcaatttc cgatacttac gcgtcgatga tggcaaccat
     7621 cgaccgcatg ggcgatgcct atagctgggt aggttacgcg gtgttgttag cgctggcgct
     7681 gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca tgctgactgg
     7741 tcatatcatg ttccagcagg cggggctgat tgccgttacg ttgtttattt tcggctactc
     7801 catgtggaca accattatct gcacggcgat tctggtttcg ctctactggg gcattacctc
     7861 caacatgatg tacaagccga ctcaggaagt gacggacggc tgcggtttct ccatcggtca
     7921 ccagcaacag tttgcatcat ggactgccta taaagttgcg ccgttcctcg gcaaaaaaga
     7981 ggaaagcgtt gaagacctca aactgccagg ctggctgaat attttccacg acaacatcgt
     8041 ctccacggcg attgtcatga ccatcttctt tggtgccatt ctgctctcct tcggtatcga
     8101 caccgtgcag gcgatggcag gcaaagtgca ctggacggtc tatatcctgc aaaccggttt
     8161 ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg cgcatgtttg tggcggaact
     8221 ctctgaagcg tttaacggta tctcccagcg cctgatccct ggtgcggttc tggcgattga
     8281 ctgtgcggct atttatagct tcgcgccgaa cgccgtggtc tggggcttta tgtggggcac
     8341 catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc ggttcctcga gcctgattat
     8401 cccaggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg ctaaccactt
     8461 cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg gggatgatcg aaatctttgg
     8521 ttgcgtctgg gcggtgaaac ttaccggtat gagtgcctgg atggggatgg cggactggtc
     8581 gattctggca ccgccgatga tgcagggctt cttctccatc ggtatcgcct ttatggccgt
     8641 catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcactgc gcgcagaaga
     8701 agatgcagaa aaacaactgg cagaacagtc tgcttaataa ggagttttga ttatgaccgt
     8761 acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga ggatgaaagt
     8821 ggaccagttt ttaacccaat caaacattga ccacacggta aatagctgcg cggttggcga
     8881 atacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca ttgcgggcga
     8941 aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc aacatgctct ctcctgccga
     9001 ctttggcccg aaactgctgg aagtgatcaa agcgcatttc ccgcaggatg tgaagtaagg
     9061 acgcgccatg aaattacgtg attcgctggc ggagaataaa tccatccgcc tgcaggctga
     9121 agctgagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg cggcggatgt
     9181 tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg gtccgtactt
     9241 tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg ttaagaaaac
     9301 cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc aaccacgaag ataacgaccc
     9361 ggtggatatc ctcatcacca tggcggcggt cgatgccaac acgcaccagg aagtgggcat
     9421 catgcagatc gtcaacctgt ttgaagatga agaaaatttc gaccgtttac gtgcctgccg
     9481 taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt aagaaggaat
     9541 tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa ccagactatg gatagcgcct
     9601 acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt accattctgt
     9661 gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctctacccg cacaaaatcg
     9721 tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct ttctcgtatg tgcttcgaag
     9781 ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat caacaccgcc aaaggtgcgc
     9841 tggacgtagc aaaagagttt aacggcgacg tgcagatcga actgaccggt tactggacct
     9901 gggaacaggc gcaacagtgg cgcgacgcag gtattcagca ggtggtttat caccgcagcc
     9961 gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga tatcactgcg atcaaacgtc
    10021 tttccgatat gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa gatctgccgc
    10081 tgttcaaagg cattccaatt catgtgttta tcgccggtcg cagcatccgt gatgccgctt
    10141 ctccggttga agccgcgcgt cagttcaaac gttccatcgc cgaactgtgg ggctaaggag
    10201 cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc cccgccgggg
    10261 agtgctggct ggaacgcctg cgactggcaa aaacgttggg cttcgatttt gtcgagatgt
    10321 cggtagatga aactgacgag cgcctgtcgc gcctcgactg gagccgcgag cagcgtctgg
    10381 cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtgcc gtccatgtgc ctttctgccc
    10441 atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg ggcgcagggg ctggagatta
    10501 tgcgtaaagc catccagttc gcccaggacg ttggcattcg cgtgatccag ctggcgggct
    10561 atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt gacggcctga
    10621 aagagagcgt tgagatggcg agccgcgcgc aggtgacgct ggcgatggag atcatggatt
    10681 atccgttgat gaactccatc agcaaggcgc tgggttacgc gcactatctc aacaacccgt
    10741 ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggacaacgat gtgcagatgg
    10801 agttgcaggc cggaatcggg catatcgtcg cggtgcatgt gaaagacacc aaaccgggcg
    10861 tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt cgaacgttgc ttcgaaacgc
    10921 tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa acggcggaag
    10981 acccggcggc agaagtggtg aaagcgcgtg attgggtgaa agcgcgcatg gccaaagcag
    11041 gcacggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag ccaacatgga
    11101 tctgccgcgc tatgggctgg tgacctttac ctggggcaac gtcagcgcta tcgaccgcga
    11161 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcagacga
    11221 tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc cgtcttccga
    11281 caccgcgacg tatctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac
    11341 ccactccact catgctactg catgggcgca ggcggggctg gcgatcccgg cgttaggcac
    11401 tacgcacgcc gactacttct ttggcgacat cccgtgtacg cgcggattaa gcgaagaaga
    11461 ggtgcagggc gagtatgaac tgaacaccgg caaagtgatt atcgaaacgc tgggtgacgc
    11521 cgagccgctg catacgccgg ggattgtggt gtatcagcac ggtccgttcg cctgggggaa
    11581 agatgcccac gacgcggtgc ataacgcggt ggtgatggaa gaagtggcga aaatggcgtg
    11641 gattgcgcgc agtattaacc cacaactgaa tcacatcgac agcttcctca tgaataaaca
    11701 cttcatgcgt aagcacggtc ctaacgccta ttacgggcag aagtaagcag tgcgccccgg
    11761 aattattacc tcctgcggga tgtaattggt ctgattccgg ggccgcttca gttaaaaaat
    11821 cgtataaaca acaggtgttt ttatggctat caatggattg tttgtcagaa cggatccgca
    11881 acgtcgccgc tatggcgttg cgttacttgt gggtgtactt tcaggaatta tttcagcatt
    11941 tgttaaatgg ggagcggaag tccccttgcc accgcgcaca ttttccggtg gtcgcgatga
    12001 atttaacccg ccgtatatct ttctacggga ttatttaggt atcgacccaa cccaaactgt
    12061 gtatacattc tcggaacata ttatcaaccc ggtgatggtg acgcatatca tcttctcact
    12121 ggtttttgcg atagggtatt gtattgtcgc ggaaattttc ccgaaagtga aattatggca
    12181 ggggatatta gcagggctta ttgtgacagt ggcagtccac ggtatatttt gcccggcgct
    12241 gaatttaacg ccgccgctca ctcaattgcc ttttgacgaa tatgcctctg aaatcctggg
    12301 gcatattttc tggttctgga taatagaaat tatgcgccgc gacttacgta atcgcattac
    12361 tcatgagcca gatccggaag tcgcaatccg gtgaataacc gcataaaaaa gccccctgcg
    12421 aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct agcgataaat
    12481 atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa ttacgtggta
    12541 gtacgttact ccccggttcc cgcatcagaa ttacgtggta gtacgttgcg ccctgcgcat
    12601 ctgtctcttc atttaatgcc tgacgcgctt cgctttcagt ggcgaaatta tgattgatat
    12661 aaatcacgcc taagctttgc acatcatcca tgtttctggc ctggtggtta ttaatttcaa
    12721 tggctgccca tgtatttgca cttagcaaaa gcacagccag aagggctaaa actcgactga
    12781 acatagatac ctcctcgacg gctgactttg tgtgctctcc tctgtgatga tcttctgatt
    12841 taattttaat caatgataaa gaagttgatg gtgaccattt ctgatgcagt tgttcaaaaa
    12901 aacaccatga tgaagtgtga tgaactccaa atcagcggtt tagaggttaa ttgcgaaagg
    12961 ggagatttat ttcggctctg cccttgagtt tagcggggca tacaagtact ataacgcgtc
    13021 atttttcagc cgacctttaa cacgttcctt gcctccccgg gattcggctg acccagacag
    13081 gaggctgaat aatccgtaag gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc
    13141 catcctgacc agagcgaaca ggttccgggc atgatcgagc gctacactgc tgccatcact
    13201 ggtgcagaag gcaagatcca ccgtctggaa gactggggcc gccgtcagct ggcttacccg
    13261 atcaacaaac tgcacaaagc acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg
    13321 atcgatgagc tggaaactac cttccgcttc aacgatgccg ttatccgcag catggttatg
    13381 cgtaccaagc acgctgttac cgaagcatct ccgatggtta aagcgaaaga cgagcgccgt
    13441 gagcgtcgcg atgatttcgc aaacgaaacc gctgatgatg ctgaagctgg ggattctgaa
    13501 gagtaatttc tgatgaccaa ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt
    13561 cgaaaggtca gtccatcagg aattcctcac tgccagttcg tgcttgagca tcgttctgtg
    13621 caggaggaag ccggttttca ccggcaggcg tggtgccaaa tgcccgttat tgttagcgga
    13681 cacgaaaacc aggccattac tcacagtata acggtcggca gtcgcataac cgttcagggg
    13741 ttcatttcat gccacaaggc aaagaacgga ctgagcaaaa tggttttgca tgccgagcag
    13801 attgaattga tagattctgg agactagcca tatggcacgt tatttccgtc gtcgcaagtt
    13861 ctgccgtttc accgcggaag gcgttcaaga gatcgactat aaagatatcg ctacgctgaa
    13921 aaactacatc accgaaagcg gtaagattgt cccaagccgt atcaccggta cccgtgcaaa
    13981 ataccagcgt cagctggctc gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta
    14041 cactgatcgc catcagtaat cggtcacggt ccattaatac gactttgaga ggataaggta
    14101 atgcaagtta ttctgcttga taaagtagca aacctgggta gcctgggtga tcaggtaaac
    14161 gttaaagcgg gctatgctcg taacttcctg gtaccgcagg gtaaagctgt tccagctacc
    14221 aagaaaaaca ttgaattctt cgaagcacgt cgcgctgaac tggaagctaa actggctgaa
    14281 gttctggcag ctgctaatgc tcgcgctgag aaaatcaatg cactggaaac tgttaccatc
    14341 gcgtctaaag ctggcgacga aggtaaactg ttcggttcca tcggtactcg cgacatcgct
    14401 gacgctgtaa ctgcagctgg cgttgaagtg gctaagagcg aagttcgtct gccgaacggc
    14461 gttctgcgta ccactggcga gcacgaagtg agcttccagg ttcacagcga agtattcgcg
    14521 aaagtgatcg taaacgtagt agctgaataa ttcgttattc aacgagacgt aaaaacgccg
    14581 accagtggtc ggcgttttgc ttttgtagcg agcacttgca gatatcgata agggccacaa
    14641 taagcagccc tacgaagata attacttagc tgcctgaata tcagccagca ttggggcaag
    14701 ggctgggttg tccttagcga agtttgcgta cattggctgt actttctcga taaacggagc
    14761 tttatctacc tggtagactt tcacgcccat tttatccagt tttgcaaatt cttgtttctc
    14821 ttcctcgctc cacaagtctt tcatgtagct cattgattct ttagctgctt ttaatataac
    14881 tgcctgattt tccgggctaa gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg
    14941 aaccatagtg tgttcgtcaa ggctgaagga ttttgccact tcaccgtgac gcatggtggt
    15001 cagcgccatc acgctgtttt ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa
    15061 ctcgccgtaa tccagtggcg tcgggttacc gcccatgact ttgatcattt caactgcgcc
    15121 agggcttggc tggacacgga ctttcatgcc tttcagatcg tctggtttca gtactggttt
    15181 attgccatag aaactgcggg cgcctccatc ataaaaagtc agcccgaaat aacctttgct
    15241 tttcgttgag tcgaggattt ttctcccgat atcgccctgc atgacctgat aataatgatc
    15301 acgatcgcgg aacaaataag gcaggctaaa taatttatag gaattttcaa atgcttccat
    15361 ttctgccgca ttggttttta ccagtggaat agcgccagaa cgaatcagct ccattgtttc
    15421 tcgttgagta cccaatgttc catttggata aatacgaata gtaatatcac cgttagagta
    15481 ctctttgttc tttttcgcaa agaactccat cgctttatga acaggatgag acttatcctg
    15541 attatgactc agttttaatg tcactgccca ggactggctt gcggataaca taagaacagc
    15601 accgataatg ccagcggata cttttatttt catagatgtt ccttttctaa taaatatggc
    15661 ggcaggtgcc gccagtgaat gcgcatcagt agcccagtgc ttttggcaat gccatactga
    15721 tttgcggaat ataggtcacc attgccagca ccaataccat cacggcatag aatggcagca
    15781 taggtttgat aattttatta atcggtaatt tagatacact acaaccgaca aataacacac
    15841 tgcctacagg cggagtacaa ataccgatac acatgttcag taccatgaca ataccaaagt
    15901 gaatcgggtc catgcccagt tcggtgacaa caggtaaaaa tatcggcgta aaaatcagta
    15961 ccgccggtgt catatccata aaagtgccga taatcaacag gataatattg ataataagca
    16021 ggattattat tgggttgtca gaaatagtgt taagcaaatc gagaatcagg aagggaacgt
    16081 cagcgtttga cattgcccat gacggtaatg actccaactt attgatagtg ttttatgttc
    16141 agataat
//
LOCUS       DAJTVE010000093        16124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000093 DAJTVE010000000
VERSION     DAJTVE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2311
                     /gene="bisC"
                     /locus_tag="OO439_002858"
     CDS             <1..2311
                     /gene="bisC"
                     /locus_tag="OO439_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6317178.1"
                     /translation="NSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSA
                     VRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREA
                     YGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGG
                     SEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLIC
                     IDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVF
                     ASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGEQK
                     HWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARIVE
                     ALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFW
                     TAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSE
                     RWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPENPD
                     SERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGNAE
                     PEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWN
                     SRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLGNG
                     CAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2280..2720)
                     /locus_tag="OO439_002859"
     CDS             complement(2280..2720)
                     /locus_tag="OO439_002859"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6317179.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2717..3280)
                     /gene="tag"
                     /locus_tag="OO439_002860"
     CDS             complement(2717..3280)
                     /gene="tag"
                     /locus_tag="OO439_002860"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6317180.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3438..4136
                     /locus_tag="OO439_002861"
     CDS             3438..4136
                     /locus_tag="OO439_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6317181.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4365..5573
                     /locus_tag="OO439_002862"
     CDS             4365..5573
                     /locus_tag="OO439_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6317182.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5897..7588
                     /gene="eptB"
                     /locus_tag="OO439_002863"
     CDS             5897..7588
                     /gene="eptB"
                     /locus_tag="OO439_002863"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6317183.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7680..7756
                     /locus_tag="OO439_002864"
     tRNA            7680..7756
                     /locus_tag="OO439_002864"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7714..7716,aa:Pro,seq:cgg)
     gene            7791..7924
                     /locus_tag="OO439_002865"
     ncRNA           7791..7924
                     /ncRNA_class="other"
                     /locus_tag="OO439_002865"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8669..10276
                     /gene="dppA"
                     /locus_tag="OO439_002866"
     CDS             8669..10276
                     /gene="dppA"
                     /locus_tag="OO439_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6317184.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10584..11603
                     /gene="dppB"
                     /locus_tag="OO439_002867"
     CDS             10584..11603
                     /gene="dppB"
                     /locus_tag="OO439_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6317185.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11613..12515
                     /gene="dppC"
                     /locus_tag="OO439_002868"
     CDS             11613..12515
                     /gene="dppC"
                     /locus_tag="OO439_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6317186.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12526..13509
                     /gene="dppD"
                     /locus_tag="OO439_002869"
     CDS             12526..13509
                     /gene="dppD"
                     /locus_tag="OO439_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6317187.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13506..14510
                     /gene="dppF"
                     /locus_tag="OO439_002870"
     CDS             13506..14510
                     /gene="dppF"
                     /locus_tag="OO439_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6317188.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14540..15811)
                     /locus_tag="OO439_002871"
     CDS             complement(14540..15811)
                     /locus_tag="OO439_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6317189.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3828 a         4027 c         4341 g         3928 t
ORIGIN      
        1 caactcatcc tcacgatatt ccgttctgac tgccgcccac tgggggccca tgctggttga
       61 aaccgacggc gaaaccgtgt ttagctcgcg tggcgcgtta gccacaggaa tggaaaactc
      121 cttgcagagc gcggttcgcg accaggttca cagcaatacg cgggtacgat ttccaatggt
      181 gcgcaaaggc tttctcgcgt caccggaaaa cccgcaaggc attcgtgggc aggatgaatt
      241 tgttcgcgtg agttgggatg aggcgctgga tcttattcac caacaacata aacgcattcg
      301 tgaggcttat ggtccggcat cgatttttgc tggttcctac ggctggcgtt caaacggcgt
      361 gctgcataag gcctcgacat tattacaacg ctatatggcg ctggcaggcg gttataccgg
      421 gcatctgggg gattattcga ccggtgcggc acaggcgatc atgccgtatg tcgtaggtgg
      481 cagcgaagtt tatcaacagc aaaccagttg gccgctggtg ctggaacata gcgatgtcgt
      541 ggtgttgtgg agtgctaacc cactcaatac gctgaaaatt gcgtggaatg catccgatga
      601 gcaggggctt tcttactttt ccgcactgcg tgacagtggg aaaaagctga tctgcattga
      661 tccaatgcga tcggaaaccg tcgatttctt tggcgataaa atggagtggg tggcaccgca
      721 catgggcacc gatgttgcgc tgatgctggg gatcgcccat acgctggtgg aaaatggttg
      781 gcacgacgaa gcgtttctgg cgcgttgcac cacaggttat gccgtcttcg cctcttattt
      841 gctgggcgag agtgacggaa tagcgaaaac cgccgaatgg gcagcagaga tttgtggtgt
      901 tggcgcagcg aaaatccgcc agctggcggc tattttccac caaaatacca ccatgctgat
      961 ggcaggctgg ggaatgcagc gccaacagtt tggtgagcaa aaacactgga tgatcgtcac
     1021 gctggcagca atgttggggc aaatcggcac acccggcggc ggttttggtc tttcttacca
     1081 ttttacgcgg cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga
     1141 tgcggtggat aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc
     1201 atatcaacac aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg
     1261 cggtgccaac tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc
     1321 ggagctggtg gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatatcgt
     1381 tctgcctgcg actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa
     1441 tcagcatctg gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt
     1501 tgatgttttt gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga
     1561 aggaaaaagt cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc
     1621 aagccagcag gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga
     1681 gatgccggaa aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc
     1741 gctggcgcat ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc
     1801 cgattacggt tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca
     1861 gggcaatgcc gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca
     1921 cagccagctg aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt
     1981 caccattcat cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt
     2041 gtggaactcc cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg
     2101 cgtgatttgc attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg
     2161 taaaaacggc gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg
     2221 ctgtgcgggt aatacggcgc tggcatggct ggaaaaatac aacggtccgg aactgacact
     2281 tacagcgttt gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc
     2341 catgcgcaat cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt
     2401 ttttgataaa cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc
     2461 ttaccaatac cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct
     2521 tccataatgc tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg
     2581 ttggcaagat aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata
     2641 aagggatgcc cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa
     2701 cgttgcgctt cccgaatcat ggtttatttc ccggatagca acagcagcca accacatgat
     2761 cattcaccag cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa
     2821 aaccacgttt tttcagtgct ttagataggg cgtcggaggc ggacgtagat gtgggaattt
     2881 cgctcaacgt tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt
     2941 cggcaaacgg ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg
     3001 cctgaatttt ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct
     3061 cttcctgcat tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt
     3121 cgcgtttttt gaggacggtg atccacgata acccagcctg ctgcccttca aggcagatca
     3181 tttcgaacag ttttttactg tcagtttcag gcacgcccca ttcattatca tggtaggcaa
     3241 tataaagcgg gtcctgactc acccagccgc aacgttccat actttccctc gctatgtacc
     3301 cgatcgtaaa aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata
     3361 cagggctact atgcccgata tccttcatac aatgacaata atattgccga attcggctct
     3421 tccctggagt cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc
     3481 tggcgagcgt ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat
     3541 atatcgttga tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc
     3601 aacctaatta caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac
     3661 tggaagtcaa tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct
     3721 ggaattttcc tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg
     3781 gcacaaccac ctcgatgtat aacgagtttg gcgacagtac taccacgcag accgatccgt
     3841 tatggcatgc cagcgtgagt accttaggct ggcgtgttga ctcccggctt ggcgatctcc
     3901 gaccctgggc gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat
     3961 caggcctgag ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag
     4021 gcgctgatat gttgctcaat caaaatattg ccgcctatgc cgcgctaact caggcagaaa
     4081 ataccactaa taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta
     4141 acagtcacaa ttgaaaccat taaataacaa taattgtggc gatagtgggt gctaacttac
     4201 caaataataa atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat
     4261 tccgttctga tggcatttca tgccgttttt ccccaggcat aaagtgcact tcgttatggt
     4321 tgtcggcaga gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt
     4381 atcagcgtac ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt
     4441 cggtttatac ctggagcctg tttaatggcg cgctttccgc caagctggat gcgccggtaa
     4501 gccaggtcgc tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg
     4561 cgggcaaatt acaggaacgt ttttgcgtta aacgcgtcac catggcttcc ggcattttgc
     4621 tgggattagg cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg
     4681 ccggtgtgct ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg
     4741 tgaagtggtt cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc
     4801 tgggtagcct gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa
     4861 aaacatttgt gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa
     4921 tgaaagacgc accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca
     4981 cgctggcaga gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg
     5041 cctgcatgag cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac
     5101 accttgatgt ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag
     5161 gtcgtctggt gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg
     5221 gtcaggtgat ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga
     5281 cgttctttgc agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc
     5341 catcactggt cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt
     5401 acctcggttt cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt
     5461 tctatgtgac cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga
     5521 cgattcgtca gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag
     5581 ccgcgagcta ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac
     5641 aatgactcgt aaaatttggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
     5701 agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca
     5761 ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
     5821 tccgcacttt tcccctgccg ggcctgaaaa gccgttaagc agggtgttat cacctgtttg
     5881 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
     5941 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag
     6001 ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac
     6061 cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg
     6121 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
     6181 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
     6241 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct
     6301 tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg
     6361 gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat
     6421 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag
     6481 ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct
     6541 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa
     6601 gaagtttaca taccaggctc cacaaaacgt tgatgagact tatgtcgtgt ttatcatcgg
     6661 tgaaaccacg cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa
     6721 actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa
     6781 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
     6841 attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta
     6901 cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca ttgcttatcg
     6961 tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt
     7021 agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca
     7081 taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa
     7141 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
     7201 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
     7261 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
     7321 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg
     7381 gatgtcagat aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa
     7441 agaagccgac atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg
     7501 tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc
     7561 gcagacaaaa gaggcagcgg ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg
     7621 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
     7681 ggtgattggc gcagcttggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
     7741 cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt tgcatttgaa
     7801 gcccatgagg atgagaacct ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac
     7861 gttgcgccag caacggcccg cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg
     7921 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catttgtagc tcatgaggat
     7981 gagaacctcc gggggcaggg aggttcgact cgacgtaggc ctgataagac gcgccagcgt
     8041 cgcatcaggc aacggcaccg catttattgt cggatgcggc gcgaccgcct tattcgacca
     8101 acacaccaca cattttcctg ccaactttgc gccccatatc tcactatcca ttaacggatt
     8161 tgtgacagaa accattgtct ttttttatat ttgcttaaat ctttttttgc gttacttatg
     8221 gctggcgctg gcattttccg ctgtgcgttt tagcgttcat ggtattaatc gctcagatat
     8281 ccaccttttc ctcagatttt tttcattaaa tgcataaaac ttaatcacca aatgttgcta
     8341 aatattaagc tgattgttct ggttatgttg gagtagcaca aatttctctg ctgcaaatag
     8401 ctgtttgaag tttttttaat ctttgtttgt ggattctcac cgttggtgta aatcccggtt
     8461 ggctgtattg acgttttcac attctgttga cagattgtag gtcacgaggg gcattttatg
     8521 gaggatccgc actgttacac tgatgttaat tagtacggca tccccacctc ataacgttga
     8581 cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa
     8641 caacaaacat cacaattgga gcagaataat gcgtatttcc ttgaaaaagt cagggatgct
     8701 gaagcttggt ctcagcctgg tggctatgac cgtcgcagca agtgttcagg ctaaaactct
     8761 ggtttattgc tcagaaggat ctccggaagg gtttaacccg cagctgttta cctccggcac
     8821 cacctatgac gcctcttccg taccgcttta taaccgcctg gttgaattta aaatcggcac
     8881 cactgaagtg atcccgggcc tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta
     8941 taccttccat ctgcgtaaag gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg
     9001 tgaactgaac gccgatgacg tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc
     9061 gtaccataaa gtctctggcg gcagctacga atacttcgaa ggcatgggct tgccggagct
     9121 gatcagtgaa gtgaaaaagg tggacgacaa cacagttcag ttcgtactga ctcgcccgga
     9181 agcgccgttc ctcgctgatc tggcaatgga cttcgcctct attctgtcaa aagaatatgc
     9241 tgatgcgatg atgaaagccg gtacaccgga aaaactggac ctcaacccaa tcggaaccgg
     9301 tccgttccag ttacagcagt accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg
     9361 ctactggggc accaaaccgc agatcgatac gctggttttc tctattaccc ctgatgcttc
     9421 cgtgcgttac gcgaaattgc agaagaatga atgccaggtg atgccgtacc cgaacccggc
     9481 agacatcgct cgcatgaagc aggataaatc catcaacctg atggaaatgc cggggctgaa
     9541 cgtcggctac ctctcctata acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca
     9601 ggcactgacc tacgcggtga acaaagacgc gatcatcaaa gcggtttatc agggcgcggg
     9661 tgtatcagcg aaaaacctga tcccgccaac catgtggggc tataacgacg acgttcagga
     9721 ttacacctac gatcctgaaa aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg
     9781 tttctccatc gacctgtggg cgatgccggt acaacgtccg tataacccga acgctcgccg
     9841 catggcggag atgattcagg cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac
     9901 ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat
     9961 gggctggact ggcgataacg gggacccgga taacttcttc gccaccctgt tcagctgcgc
    10021 cgcctctgaa caaggttcta actactcaaa atggtgctac aaaccgtttg aagatctgat
    10081 tcaaccggcg cgtgctaccg acgaccataa caaacgcgtt gaactgtaca agcaagcgca
    10141 ggtggtgatg cacgatcaga ctccggcact gatcatcgct cactccaccg tgtttgaacc
    10201 ggtacgcaaa gaagtcaaag gctatgtggt tgatccatta ggcaaacatc acttcgaaaa
    10261 cgtctctatc gaataattaa aagccataca agactgatgg caaaggcaaa actgcctgat
    10321 gtgctccgct tatcaggcct acgaaaattc tgcaatgtat tgaatttgca cgattttgta
    10381 ggccggataa ggcgttaacg ccgcatccgg cataaacaaa gcgcactttg tcaacaatct
    10441 gtatacccgg tggcgatgtg cctctgcgtg cagcgccatc cggcagcaat acttcattcc
    10501 ctgtccgata agggcgggaa ttgatttgtg agcaatacag acacgcagtt ccaggctgcg
    10561 ggtcactata gagaatccgg gttatgttgc agtttattct ccgacgtttg ggactcgtca
    10621 tccccacgtt tatcggtatt acccttctca catttgcctt tgtccacatg atcccgggcg
    10681 atccggtgat gatcatggca ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc
    10741 tggctgaact cggcttagat aaaccgatgt ggcagcagta tctccattac atttggggcg
    10801 taatgcacgg cgatctaggc atttcaatga aaagccgaat tccagtatgg gaagagttcg
    10861 tgccgcgctt tcaggccacg ctggaacttg gcgtctgcgc gatgattttt gctacggcag
    10921 tcggtattcc ggttggtgtg ctggctgcgg ttaaacgcgg ttccattttc gatcacactg
    10981 cagttggcct ggcgctgacc ggctactcga tgccgatctt ctggtggggc atgatgctga
    11041 tcatgctggt ttcggtgcac tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg
    11101 tgttcctcga tgactccaat ccgttaaccg gttttatgct gatcgacacc gccatctggg
    11161 gtgaagatgg caactttatc gacgccgttg cccatatgat cttaccggct attgtgctgg
    11221 gtactatccc gctggcggtt atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg
    11281 gtgaagatta catccgcacc gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg
    11341 tccatgcgct gcgtaacgcg atgctgccgg tagtgaccgt tatcggcctg caggtgggaa
    11401 cattgctggc gggggcgatt ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct
    11461 ggttgattga cgcactgcaa cgccgcgact atccggtagt gcagggcggc gtattgctgg
    11521 tggcgacgat gattatcctc gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc
    11581 cgcgtattcg tcataagaag taaggggaca tcatgtcaca ggttactgaa aataaagtga
    11641 ttagcgcacc ggtgccgatg accccgttac aggagttctg gcactatttt aaacgcaaca
    11701 aaggcgcggt tgtcgggctg gtttacatcg tcatcgtgct gtttatcacg atctttgcca
    11761 actggattgc gccttataac ccggcggatc agttccgcga tgcgctgctc gccccgccag
    11821 cctggcagga aggcggcagc atggcgcact tgctgggcac cgatgacgta ggccgtgatg
    11881 tgctgtcgcg gctgatgtac ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg
    11941 tgttatcgct gattatgggc gttattctcg gcctgatcgc cggttacttc ggcggcctgg
    12001 tcgataacat catcatgcgc gtggtcgata tcatgctggc gctgccaagt ctgctgttgg
    12061 cactggtgct ggtggcgatt ttcggcccgt cgattggtaa cgccgcgctg gcactgacct
    12121 tcgttgcctt gccgcactat gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc
    12181 gcgattacgt taccgcgtct cgcgtggcag gtgccggggc gatgcgtcag atgtttatta
    12241 acatcttccc gaactgcctg gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg
    12301 ccattctcga tatggccgcc cttggcttcc tcggcatggg agcgcagccg ccaacaccag
    12361 agtggggcac catgctctcc gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga
    12421 ccttcccggg tctggcgatc ctgctgacgg tgctggcatt taacctgatg ggtgacggtc
    12481 tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt tcgagatggc gttattaaat
    12541 gtagataaat tatcggtgca tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc
    12601 atcagctaca gcgtaaaaca gggcgaagtg gtcgggattg tgggtgagtc tggctccggt
    12661 aagtcggtca gttcactggc gattatgggg ctgattgatt atccgggccg cgtaatggcg
    12721 gaaaagctgg tgtttaacgg tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac
    12781 ctggtgggtg ccgaagtggc gatgatcttc caggacccga tgaccagcct taacccgtgc
    12841 tacaccgtgg gtttccagat catggaagcg attaaggtgc atcagggcgg taataagagc
    12901 actcgccgtc agcgggcgat tgacctgctg aatcaggtcg gtattcccga tccggcatcg
    12961 cgtctggatg tttacccgca tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc
    13021 atggcgattg cctgtcggcc aaaactgctg attgccgatg agccgaccac tgcgctggac
    13081 gtgaccattc aggcgcaaat catcgaactg ttgctggagc tacagcagaa agagaacatg
    13141 gcgctggtgt taattaccca tgatctggcg ctggtggcgg aagctgcgca taaaatcatt
    13201 gtgatgtatg caggccaggt ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg
    13261 cgtcacccgt atactcaggc attgctgcgt gcgctgccag aatttgctca ggacaaagaa
    13321 cgtctggcgt cgttgccagg tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg
    13381 cttaacccgc gctgccccta tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat
    13441 atgctcgctg acgggcgcca gtccaaatgc cattacccac ttgatgatgc cgggaggccg
    13501 acactatgag tacgcaagag gccaccctgc aacaaccgct gttgcaggct atcgacctga
    13561 aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg
    13621 atggcgtttc gtttaacctt gaacgtggca aaacgctggc agtagtgggc gaatctggct
    13681 gcggtaaatc gaccctcggt cggttgctga cgatgattga aacgcccacc ggtggcgagc
    13741 tgtattacca ggggcaggat ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc
    13801 agaaaatcca gatcgtcttc cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg
    13861 ggcaaattct tgaagagccg ctgctgatca acaccagctt aagcaaagaa cagcgtcggg
    13921 aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc
    13981 cgcatatgtt ctccggcggt cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg
    14041 acccggatgt ggtgattgcc gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc
    14101 aggtgctgaa tctgatgatg gatttgcagc aggagttggg gctgtcttat gtctttatct
    14161 cccacgacct gtcggtggtg gagcacattg ctgatgaagt gatggtgatg tacctgggcc
    14221 gctgcgtgga gaagggaacg aaagaccaaa tcttcaataa cccgcgccat ccgtacactc
    14281 aggcgctact ttccgcgacg ccgcgcctga acccggacga tcgccgcgag cgcatcaagc
    14341 tcagcggtga actaccaagc ccactgaatc caccgccggg ttgcgccttc aacgcccgct
    14401 gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca gctaaaagac tacggcggtc
    14461 aactggtagc ttgttttgct gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac
    14521 cggggccatg cgctccggtt tactcaatca gcttcaacag tggcgacaag catagcaaaa
    14581 tgccgtacag cagaatcagc caggctttaa atccgcgaag tttttccagt tgtgcgactt
    14641 tatagatcag gaaaaacgga atgaggcacg acacaatacc atataacggg cttcccaact
    14701 gaaagaacac cagcaccgat acacgaaacg aaacccaaat cgtcaacgta atgacgataa
    14761 aagcgcagat cgccagagtc agcacgcgtg agttaatttt cttggtatca ataattcggc
    14821 ttaacagatt gagaataatg cctttaatgg cctcgtggaa accgagataa atgccaaaga
    14881 atgcggtcag tacggcaaag atattaagca ccgtagaggt gatatgaatg atatgcccag
    14941 ggatcacctg cgcggccagc gccagtgctg agatattttg ttcaaaggca gaaacggctt
    15001 cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg
    15061 tgatataact aattcggtgg gtacgcagcg cgagccgggt tgccagtact ttatccgctt
    15121 cccgtttacg ataggcaata ttcattgggt taagtacctg aataaatact gcagaaaaga
    15181 agcaaaatgg aatggtaagc aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg
    15241 ttatattggc gaaattccag tgcgggatca tcgcaaaacc gaacacgaca ataatcccta
    15301 ctttgaccac caccattggc ccggaaatct taaacagtaa tcgttcacca ccagacgcaa
    15361 tcgctaccag tacggcgaaa atagcgactt tataaagtag agattgtgaa agatcggcat
    15421 cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa aaccacggag agagagtaga
    15481 taaaaatccc gtggataatc atcaaaaagt agataacccc gaggaaaatt ccccagttct
    15541 tccccaggta atgactgata atatcggtgt agtcattaca ggaatcgctt tcagaaaggg
    15601 tttttaaata aatgtcctgc actacccagg tggcaggata agcaatgatc gctgcggtaa
    15661 taaataccca aattcccttc aagccaattt gtactggcat cagcacggtt ccggcaccaa
    15721 tcgccatgcc tatgcataat aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct
    15781 gattgtgttt cgatagtgtg ttgtgctgca taaagtcctg tccagaaaga agtcggcctc
    15841 gcaaaaatga ataaacccag tttctcttgc gcaacaggtg gagaagtcga atacgcagta
    15901 tgtaaaaaat gaggcaacag attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa
    15961 ttgcgcattt cactaccagt ctggagtcag cccggatatg aactgcaact attgcaaata
    16021 aagaaattaa gctggaaggc atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa
    16081 agcaatcgta aaatcagcgg caagccgaaa gggttccggt gagg
//
LOCUS       DAJTVE010000094        16073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000094 DAJTVE010000000
VERSION     DAJTVE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..566)
                     /locus_tag="OO439_002872"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="OO439_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO439_002873"
                     /pseudo
     CDS             complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO439_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO439_002874"
     CDS             complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO439_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6317190.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3071..3289
                     /locus_tag="OO439_002875"
     CDS             3071..3289
                     /locus_tag="OO439_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6317191.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3297..4238
                     /gene="ribF"
                     /locus_tag="OO439_002876"
     CDS             3297..4238
                     /gene="ribF"
                     /locus_tag="OO439_002876"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6317192.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4281..7097
                     /gene="ileS"
                     /locus_tag="OO439_002877"
     CDS             4281..7097
                     /gene="ileS"
                     /locus_tag="OO439_002877"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6317193.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7097..7591
                     /gene="lspA"
                     /locus_tag="OO439_002878"
     CDS             7097..7591
                     /gene="lspA"
                     /locus_tag="OO439_002878"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6317194.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7716..8165
                     /gene="fkpB"
                     /locus_tag="OO439_002879"
     CDS             7716..8165
                     /gene="fkpB"
                     /locus_tag="OO439_002879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6317195.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8167..9117
                     /gene="ispH"
                     /locus_tag="OO439_002880"
                     /gene_synonym="lytB"
     CDS             8167..9117
                     /gene="ispH"
                     /locus_tag="OO439_002880"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6317196.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9183..10097
                     /gene="rihC"
                     /locus_tag="OO439_002881"
     CDS             9183..10097
                     /gene="rihC"
                     /locus_tag="OO439_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6317197.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10264..11085
                     /gene="dapB"
                     /locus_tag="OO439_002882"
     CDS             10264..11085
                     /gene="dapB"
                     /locus_tag="OO439_002882"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6317198.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11541..12689
                     /gene="carA"
                     /locus_tag="OO439_002883"
     CDS             11541..12689
                     /gene="carA"
                     /locus_tag="OO439_002883"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6317199.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12707..15928
                     /gene="carB"
                     /locus_tag="OO439_002884"
     CDS             12707..15928
                     /gene="carB"
                     /locus_tag="OO439_002884"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6317200.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3922 a         3782 c         4254 g         4115 t
ORIGIN      
        1 tcatcccccc agtagaaagg aatggagata aacagattaa aacgctcctc ttctttatta
       61 ttttcatcat aagattggct ggcagagaaa gtataactaa tgtgttgcca gttattggag
      121 taactgaatt ggtaatcttt agcatttcca cttcgccccc agtaattccg ccaaagcgca
      181 cttaatgata catttcccag attattggat aaaggttgca taatattggc agaaaggcta
      241 ttttttctgc caatatcata aaaatcatca taatcactct gatgatagtg tttatcattt
      301 tcgtaaagat ggtcgctgaa tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg
      361 ctaaatcgag tggctgtttg tactagatat ttattatagg cgacctgata gctcgtacct
      421 tcgtgtctgg tatcattatt taacttacta ctcgatcggg cagcgtaaaa agagatggca
      481 cccagcggtg tattccaacc atttcccaga gttatagcat tatagttatc ggataaaatc
      541 gtacccgatt atgtgatcag gttgttatta tttagattaa tgtttaaatt ttttaatttc
      601 ttgatgtgaa gttgatgaaa gtttgaaaat ttaatattct taattgttcc cttctttata
      661 agaagagcaa ggtgccatca ttagagtgac agagtaggat gtttcagagc atgcttcaga
      721 ttgtttttta tttctctgca gtgtattaat aatcatcaca gataatttca ttctaaaatt
      781 acctctttct ttgagtgttt taatcctgat gaactatatt ttttataagg agcatgcact
      841 cgttgatgct ctgaaggtat tttctcattt ttgatgatgt tttgtcgtaa tcgtgcataa
      901 acttattaag ttctttatac acatcattat ttagctttac attatcgcga actaaagttt
      961 gtaccaatgg tatcaaaatt tcgaaacggg acggtttgtc atcaatactt gccagggttt
     1021 tgaatactcc tgtgtatctg taacctaagt gcttattgac aagttgagtg gtccatcggc
     1081 caggcataat atcttcacag cgcaaatttt ttatcatggt attagtgaac gatgttccaa
     1141 atatgttgct ttttttaatg aagaggttgt ttatttctcc atttgaaaag atggtgtttg
     1201 ataagtcgct ttcaaaacat gatatattgg ttaatacaca acaaaaataa tacccatatg
     1261 aaaggttgct cttttctatt gttatatttg atagcaacat tttatgtata ttaatattaa
     1321 gagcagtcaa atactggatc ttttgatcgt taagttcata catctccagt gatttattat
     1381 agggtaataa aagtataaat tcctttatat tatctccgca gtaagaaaag acgacattaa
     1441 atttattagt ggccatgttt tcagttgagc agtaagctga tttttctatt aaccgactgg
     1501 gcacgctttt aaataaagat aattttccac atagttttga atatgccgaa ttgctagttt
     1561 taaagttctg ccacattatt ccctggttat catctatcga tgaggagggg attttattgc
     1621 attgctcatt gtatagctca gggaaaatat tttttttaat aagctttttg atttcctggt
     1681 taagatgact ttttttcttg tctattgcac tgtattttgc gttttgtgac gtttcctgat
     1741 gatcataaaa tgactggaga tttctgcagg cctcataaaa tatttcagga gtatatagct
     1801 gtgggtatat ttcttggttg aagtttgcct ttaaaaaatt aacgaatttt tcatggcatt
     1861 cttggtatat cttaatctct tcagaaagac attcgcttgt tctgtcatca aaaacatcca
     1921 atctaagtaa tgtgatacct ttgtgtttaa aaaataaaat tgaactgtat gttctatgag
     1981 tttacatcta taaaaatatg catctctcag atgagtatat tgaaaaaaat gatgaaggac
     2041 tggagatggt ttaggcaagt tatggcttct aaaaaagtca tcaaacaaaa ttaagaactc
     2101 tgttttatac tcgcaaaata cttttactaa ctcattttgt cttgttttat ttattattac
     2161 atcatctact atatttgatt tataaagaca atgtggcgaa gttgagatac catttgtcat
     2221 tatcagtcct tgaagttgat gaatttttat taatttaatt tatgagtgaa tgtgactact
     2281 atcaaaaata gctcattaat tttatgaagt agaatataaa taggtattta acctgtgttg
     2341 attgttgtta attatttaca agatctaata aggagatatt tatgaatggt taaagtgtat
     2401 aggaatttaa ttcaaaaata gagtggtctt aagtttaata cctcaatgtg tatcacaata
     2461 tccacattct ttgtagggga gtctggagat tgagtagata ttcttgttca gaatgtatca
     2521 gccgatggtt ctacgattct taagccacga agagttcaga aagtgtaacg gcatgtctgc
     2581 aaaagccagc atcttttgac tatctggcaa ccggtagtgt gttctctcac gcatcataaa
     2641 agcagcaggc ataaaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
     2701 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
     2761 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
     2821 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
     2881 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
     2941 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
     3001 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
     3061 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
     3121 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
     3181 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
     3241 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
     3301 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     3361 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     3421 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     3481 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     3541 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     3601 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     3661 ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     3721 aagctagcat ggaatacggc ttcgatatca ctagtacgca aactttttgc gaaggtggcg
     3781 tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     3841 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     3901 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     3961 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     4021 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     4081 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     4141 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     4201 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     4261 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     4321 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     4381 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     4441 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     4501 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     4561 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     4621 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     4681 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     4741 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     4801 gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg
     4861 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     4921 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     4981 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     5041 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     5101 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     5161 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     5221 acgttccggc aatcctcggc gatcacgtta ccctagatgc cggtaccggt gccgttcaca
     5281 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     5341 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     5401 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     5461 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     5521 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     5581 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     5641 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     5701 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     5761 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     5821 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     5881 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     5941 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     6001 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     6061 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     6121 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     6181 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     6241 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     6301 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     6361 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     6421 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     6481 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     6541 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     6601 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     6661 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga
     6721 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     6781 gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg
     6841 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     6901 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     6961 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     7021 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     7081 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     7141 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     7201 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     7261 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     7321 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     7381 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     7441 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     7501 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     7561 ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggatgcga tgctgacgca
     7621 tcttatccgg cctacagatt gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaaaa tcctcaaata taagagcaaa cctgcatgtc tgaatctgta cagagcaata
     7741 gcgccgtcct ggtgcacttc acgctaaaac tcgacgatgg caccaccgcc gagtctaccc
     7801 gcaacaacgg taaaccggcg ctgttccgcc tgggtgatgc ttctctttct gaagggctgg
     7861 agcaacacct gttggggctg aaagtgggcg ataaaaccac cttctcgttg gagccagatg
     7921 cggcgtttgg cgtgccgtca ccggacctga ttcagtactt ttcccgccgt gaatttatgg
     7981 atgcaggcga gccagaaatt ggcgcaatca tgctttttac cgcaatggat ggcagtgaga
     8041 tgcctggcgt gatccgcgaa attaacggcg actccattac cgttgatttc aaccatccgc
     8101 tggccgggca gaccgttcat tttgatattg aagtgctgga aatcgatccg gcattggagg
     8161 cgtaacatgc agatcctgtt ggccaacccg cgtggttttt gtgccggggt agaccgcgct
     8221 atcagcattg ttgaaaacgc gctggccatt tacggcgcac cgatatatgt ccgtcacgaa
     8281 gtggtacata accgctatgt ggtcgatagc ttgcgtgagc gtggggctat ctttattgag
     8341 cagattagcg aagtaccgga cggcgcgatc ctgattttct ccgcacacgg tgtttctcag
     8401 gcggtacgta acgaagcaaa aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg
     8461 gtgaccaaag tgcatatgga agtcgcccgc gccagccgtc gtggcgaaga gtcgattctc
     8521 atcggccacg ccgggcatcc ggaagtggaa ggtacaatgg gccagtacag taacccggaa
     8581 gggggaatgt atctggtcga atcgccggac gatgtgtgga aactgacggt caaaaacgaa
     8641 gagaagctct cctttatgac ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc
     8701 gacgcgctgc gtaaacgctt cccgaaaatt gtcggtccgc gcaaagatga catctgctac
     8761 gccacgacta accgtcagga agcggtacgc gccctggcag aacaggcgga agttgtgttg
     8821 gtggtcggtt cgaaaaactc ctccaactcc aaccgtctgg cggagctggc ccagcgtatg
     8881 ggcaaacgcg cgtttttgat tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag
     8941 gttaaatgcg tcggcgtgac tgcgggcgca tcggctccgg atattctggt gcaaaatgtg
     9001 gtggcacgtt tgcagcagct gggcggtggt gaagccattc cgctggaagg ccgtgaagaa
     9061 aacattgttt tcgaagtgcc gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc
     9121 attagcagcc taagttatgc gaaaatgccg gtcttgttac cggcattttt tatggagaaa
     9181 acatgcgttt acctatcttc ctcgatactg accccggcat tgacgatgcc gtcgccattg
     9241 ccgccgcgat ttttgccccc gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg
     9301 tctcggttga gaaaaccacc cgcaacgccc tgcaactgct gcatttctgg aatgcggaga
     9361 ttccgctcgc ccagggagcc gctgtgccgc tggtacgcgc accgcgtgat gcagcatctg
     9421 tgcacggcga atcgggaatg gctggctacg actttgttga gcacaaccga aagccaatcg
     9481 ggataccagc gtttctggcg attcgtgatg ccctgatgcg tgcaccagag cctgttaccc
     9541 tggtggccat tggcccgtta accaatattg cgctgttact ttcacaatgc ccggaatgta
     9601 aaccgtatat tcgtcgtctg gtgatcatgg gcggttcttc tggtcgcggc aactgtacac
     9661 caaatgccga gtttaatatc gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg
     9721 gaatcgaaat cgtcatgtgc ggtctggatg tcaccaatca ggcaatatta acccctgact
     9781 atctcgctac tttgccggag ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc
     9841 actaccgtag cggcagtatg caaagcggtc tgcgaatgca cgatctctgc gccatcgcct
     9901 ggctggtgcg cccggactta ttcaccctca agccgtgttt tgtcgcggta gaaactcaag
     9961 gcgaatttac ctcaggcacg acggtggttg atatcgacgg ttgcctgggc aaaccagcca
    10021 atgtaaaggt ggcattggat ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc
    10081 tggctctggc gtcgtaacct gtcacatgtt atcggcatgc agtcattcat cgactcatgc
    10141 ctttcactga tatccctccc tgtttatcat taatttctaa ttatcagcgt ttttggctgg
    10201 cggcgtagcg atgcgctggt tactctgaaa acggtctatg caaattaaca aaagagaata
    10261 gctatgcatg atgcaaacat ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc
    10321 cagttgattc aggcggcgct ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc
    10381 gaaggatctt ctttactggg cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc
    10441 gttaccgtgc aaagcagcct cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt
    10501 acccgtccgg aaggtacgct gaaccatctc gctttttgtc gccagcatgg caaagggatg
    10561 gtgatcggca ctacggggtt tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc
    10621 gatattgcga ttgtctttgc cgccaatttt agcgttggcg ttaacgtcat gcttaagctg
    10681 ctggagaaag cagccaaagt gatgggtgac tacaccgata tcgaaattat tgaagcacat
    10741 catagacata aagttgatgc gccgtcaggc accgcactgg caatgggaga ggcgatcgcc
    10801 cacgcccttg ataaagatct gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt
    10861 gaacgtgtgc ctggcaccat tggttttgcc accgtgcgtg caggtgacat cgttggtgaa
    10921 cataccgcga tgtttgccga tattggcgag cgtctggaga tcacccataa ggcgtccagc
    10981 cgtatgacat ttgctaacgg cgcggtaaga tcggctttgt ggttgagtgg taaggaaaat
    11041 ggtctttttg atatgcgaga tgtacttaat ctcaataatt tgtaaccaca aaatatttgt
    11101 tatggtgcaa aaataacaca tttaatttat tgattataaa gggctttaat ttttggccct
    11161 tttattttta gtgttatgtt tttaaatatt ctataagtgc caaaaattac atgttttgtc
    11221 ttctattttt gttgttttaa tgtaaatttt gaccatttgg tccacttttt cctgctcgtt
    11281 tttatttcat gcaatcttct tgctgcgcaa gcgttttcca gaacaggtta tatgatcttt
    11341 ttgtcgctta atgcctgtaa aatatgcatg agccacaaaa taatataaaa aataccgcca
    11401 ttaagttgac ttttagcgcc catatctcca gaatgccgcc gtttgccaga aattcgtcgg
    11461 taagcagatt tgcattgatt tacgtcatca ttgtgaatta atatgcaaat aaagtgagtg
    11521 aatattctct ggagggtgtt ttgattaagt cagcgctatt ggttctggaa gacggaaccc
    11581 agtttcacgg tcgggccata ggggcaacag gttcggcggt tggggaagtc gttttcaata
    11641 cttcaatgac cggttatcaa gaaatcctca ctgatccttc ctattctcgt caaatcgtta
    11701 ctcttactta tccccatatt ggcaatgtcg gcactaatga cgccgatgaa gaatcttctc
    11761 aggtacatgc acaaggtctg gtgattcgcg atctgccgct gattgccagc aacttccgta
    11821 ataccgaaga cctctcttct tacctgaagc gccataacat cgtggcgatt gccgatatcg
    11881 atacccgtaa gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg
    11941 caggcgataa cccggatgcg gcgttggcgt tagaaaaagc ccgcgcgttc ccaggtctga
    12001 atggcatgga tctggcaaaa gaagtgacca ccgcagaagc ctatagctgg acacaaggga
    12061 gctggacgtt gaccggcggc ctgccagaag cgaaaaaaga agacgagctg ccgttccacg
    12121 tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg gatagaggct
    12181 gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag
    12241 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg
    12301 ccatccagaa attcctcgaa accgatattc cggtattcgg catctgcctc ggtcatcagc
    12361 tgctggcgct ggcgagcggt gcgaagactg tcaaaatgaa atttggtcac cacggcggca
    12421 accatccggt taaagatgtg gagaaaaacg tggtgatgat caccgcccag aaccacggtt
    12481 ttgcggtgga tgaagcaaca ttacctgcaa acctgcgtgt cacgcataaa tccctgttcg
    12541 acggtacgtt acagggcatt catcgcactg ataaaccggc gttcagcttc cagggtcacc
    12601 cggaagccag ccctgggcca cacgacgcag cgccgttgtt cgaccacttt atcgagttaa
    12661 ttgagcagta ccgtaaaacc gctaagtaat caggagtaaa agagccatgc caaaacgtac
    12721 agatataaaa agtatcctga ttctgggtgc gggcccgatt gttatcggtc aggcgtgtga
    12781 gtttgactac tctggcgcgc aagcatgtaa agccctgcgc gaagagggtt accgcgtcat
    12841 tctggtgaac tccaacccgg cgaccatcat gaccgacccg gaaatggccg atgcgaccta
    12901 catcgagccg attcactggg aagtagtacg caagattatt gaaaaagagc gcccggacgc
    12961 ggtgctgcca acgatgggcg gtcagacggc gctgaactgc gcgctggagc tggagcgtca
    13021 gggcgtgttg gaagagttcg gcgtgactat gattggtgcg accgccgatg cgattgataa
    13081 agcagaagac cgccgtcgtt tcgacgtagc gatgaagaaa attggtctgg aaaccgcgcg
    13141 ttccggtatc gcacacacga tggaagaagc gctggcggtt gccgctgacg tgggcttccc
    13201 gtgcattatt cgcccatcct ttaccatggg cggtagcggc ggcggtatcg cttataaccg
    13261 cgaagagttt gaagaaattt gcgcccgcgg tctggatctc tccccaacca aagagctgct
    13321 gattgatgag tcgctgatcg gctggaaaga gtacgagatg gaagtggtgc gtgataaaaa
    13381 cgacaactgc atcatcgtct gctctatcga aaactttgat gcgatgggca tccataccgg
    13441 tgactccatc actgtcgcgc cagcccaaac gctgaccgac aaagaatatc aaatcatgcg
    13501 taacgcctcg atggcggtgc tgcgtgaaat cggcgttgaa accggtggtt ccaatgtcca
    13561 gtttgcggtg aacccgaaaa acggtcgcct gattgttatc gaaatgaacc cacgcgtgtc
    13621 ccgttcttcg gcgctggcgt cgaaagcgac cggtttcccg attgctaaag tggcggcgaa
    13681 actggcggtg ggttacaccc tcgacgaact gatgaacgac atcactggcg gacgtactcc
    13741 ggcctccttc gagccgtcca tcgactatgt ggttactaaa attcctcgct tcaacttcga
    13801 aaaattcgct ggtgctaacg accgtctgac cactcagatg aaatcggttg gcgaagtgat
    13861 ggcgattggt cgcacgcagc aggaatccct gcaaaaagcg ctgcgcggcc tggaagtcgg
    13921 tgcgactgga ttcgacccga aagtgagcct cgatgacccg gaagcgttaa ccaaaatccg
    13981 tcgcgaactg aaagatgctg gcgcagagcg tatctggtac atcgccgatg ccttccgtgc
    14041 gggcctgtct gtggacggcg tgttcaacct gaccaatatt gaccgctggt tcctggtaca
    14101 gattgaagaa ctggtgcgtc tggaagagaa agtagcggaa gtgggcatca ctggcctgaa
    14161 cgctgaattc ctgcgccagc tgaaacgcaa aggctttgcc gatgcgcgtc tggcaaaact
    14221 ggcgggcgtg cgcgaagcgg aaatccgcaa gctgcgcgac cagtatgacc tgcacccggt
    14281 ttataagcgc gtggatacct gtgcggcaga gttcgccacc gacaccgctt acatgtactc
    14341 cacttatgaa gaagagtgcg aagcgaatcc gtctaccgac cgtgaaaaaa tcatggttct
    14401 cggcggcggc ccgaaccgta tcggtcaggg tatcgaattc gactactgct gtgtacacgc
    14461 ctcgctggcg ctgcgcgaag acggttacga aaccattatg gttaactgta acccggaaac
    14521 cgtctccacc gactacgaca cttccgatcg cctctacttc gagccggtaa ctctggaaga
    14581 tgtgctggaa atcgtgcgta ttgagaagcc gaaaggcgtt atcgttcagt acggcggtca
    14641 gaccccgctg aaactggcgc gcgcgctgga agctgctggc gtaccggtta tcggcaccag
    14701 cccggatgct atcgaccgtg cagaagaccg tgaacgcttc cagcatgcgg ttgagcgtct
    14761 gaaactgaaa caaccggcga acgccaccgt taccgctatt gaaatggcgg tagagaaggc
    14821 gaaagagatt ggctacccac tggtggtacg tccgtcttac gttctcggcg gtcgggcgat
    14881 ggaaatcgtc tatgacgaag ctgacctgcg tcgctacttc cagacggcgg tcagcgtgtc
    14941 taacgatgcg ccagtgttgc tggatcactt ccttgatgac gcggtagaag ttgacgtgga
    15001 tgccatctgc gacggcgaaa tggtgctgat tggcggcatc atggagcaca tcgagcaggc
    15061 gggcgtgcac tccggtgact ccgcatgttc tctgccagcc tacaccttaa gtcaggaaat
    15121 tcaggatgtg atgcgccagc aggtgcagaa actggccttc gaattgcagg tgcgcggtct
    15181 gatgaacgtg cagtttgcgg tgaaaaacaa cgaagtctac ctgattgaag ttaacccgcg
    15241 tgcggcgcgt accgttccgt tcgtctccaa agccaccggc gtaccgctgg caaaagtggc
    15301 ggcgcgtgtg atggctggca aatcgctggc tgagcagggc gtaaccaaag aagttatccc
    15361 gccgtactac tcggtgaaag aagtggtgct gccgttcaat aaattcccgg gtgttgaccc
    15421 gctgttaggg ccagaaatgc gctccaccgg ggaagtgatg ggcgtgggcc gcaccttcgc
    15481 tgaagcgttt gccaaagcgc agctgggcag caactccacc atgaagaaac acggtcgtgc
    15541 gttgctttcc gtgcgcgaag gcgataaaga acgcgtggtg gacctggcgg caaaactgct
    15601 gaaacagggc ttcgagctgg atgcgaccca cggcacggcg attgtgctgg gcgaagcggg
    15661 cattaatccg cgtctggtga acaaggtgca tgaaggccgt ccgcacattc aggaccgcat
    15721 caagaatggc gaatatacct acatcatcaa caccacttca ggccgtcgcg cgattgaaga
    15781 ctcccgcgtg atccgtcgca gtgcgctgca atataaagtg cattatgaca ccaccctgaa
    15841 cggtggtttc gctaccgcga tggcgctgaa tgccgacgcg accgaaaaag taatttcggt
    15901 gcaggaaatg cacgcgcaga tcaaataata gcgtgtcatg gcggatattt ttcatccgct
    15961 aatttgatcg aataagtaat acggttctct gatgaggacc gttttttttg cccattaatt
    16021 aaatcttttg gggaatcgat atttttgatg acataagcag gatttagctc aca
//
LOCUS       DAJTVE010000095        15641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000095 DAJTVE010000000
VERSION     DAJTVE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1223
                     /locus_tag="OO439_002885"
                     /pseudo
     CDS             1..1223
                     /locus_tag="OO439_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1311..2282)
                     /gene="lpxM"
                     /locus_tag="OO439_002886"
                     /gene_synonym="msbB"
     CDS             complement(1311..2282)
                     /gene="lpxM"
                     /locus_tag="OO439_002886"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6317201.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2402..3724)
                     /gene="mepM"
                     /locus_tag="OO439_002887"
     CDS             complement(2402..3724)
                     /gene="mepM"
                     /locus_tag="OO439_002887"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6317202.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3740..4672)
                     /gene="znuA"
                     /locus_tag="OO439_002888"
     CDS             complement(3740..4672)
                     /gene="znuA"
                     /locus_tag="OO439_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6317203.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4751..5506
                     /gene="znuC"
                     /locus_tag="OO439_002889"
     CDS             4751..5506
                     /gene="znuC"
                     /locus_tag="OO439_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6317204.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5503..6288
                     /gene="znuB"
                     /locus_tag="OO439_002890"
     CDS             5503..6288
                     /gene="znuB"
                     /locus_tag="OO439_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6317205.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6435..7445)
                     /gene="ruvB"
                     /locus_tag="OO439_002891"
     CDS             complement(6435..7445)
                     /gene="ruvB"
                     /locus_tag="OO439_002891"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6317206.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7454..8065)
                     /gene="ruvA"
                     /locus_tag="OO439_002892"
     CDS             complement(7454..8065)
                     /gene="ruvA"
                     /locus_tag="OO439_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6317207.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8204..8269)
                     /gene="yobI"
                     /locus_tag="OO439_002893"
     CDS             complement(8204..8269)
                     /gene="yobI"
                     /locus_tag="OO439_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6317208.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8342..8944
                     /locus_tag="OO439_002894"
     CDS             8342..8944
                     /locus_tag="OO439_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6317209.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(8946..9467)
                     /gene="ruvC"
                     /locus_tag="OO439_002895"
     CDS             complement(8946..9467)
                     /gene="ruvC"
                     /locus_tag="OO439_002895"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6317210.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9502..10242)
                     /locus_tag="OO439_002896"
     CDS             complement(9502..10242)
                     /locus_tag="OO439_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6317211.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10271..10723)
                     /gene="nudB"
                     /locus_tag="OO439_002897"
     CDS             complement(10271..10723)
                     /gene="nudB"
                     /locus_tag="OO439_002897"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6317212.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(10841..12613)
                     /gene="aspS"
                     /locus_tag="OO439_002898"
     CDS             complement(10841..12613)
                     /gene="aspS"
                     /locus_tag="OO439_002898"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6317213.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            12923..13489
                     /locus_tag="OO439_002899"
     CDS             12923..13489
                     /locus_tag="OO439_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6317214.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            13844..14092
                     /locus_tag="OO439_002900"
     CDS             13844..14092
                     /locus_tag="OO439_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6317215.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14693..>15641
                     /locus_tag="OO439_002901"
     CDS             14693..>15641
                     /locus_tag="OO439_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6317216.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFE"
BASE COUNT         3953 a         3989 c         3604 g         4095 t
ORIGIN      
        1 aagatccgca cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa
       61 acaacaactg gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga
      121 ataagatcgt aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac
      181 ttcttttttc agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat
      241 acattactga aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg
      301 cggcctggga ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa
      361 aaatgacaac tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt
      421 cctcttatct tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa
      481 cgtttattga ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac
      541 aaacatctct tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg
      601 atgattcgtt cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg
      661 atcgagaaac tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc
      721 agacgcttga attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag
      781 acaattatga taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat
      841 tctccttatt tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata
      901 taatgaatca tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt
      961 cactatcatt ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg
     1021 aagacggcga cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca
     1081 tgctttcgtg agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt
     1141 tagatgacag tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta
     1201 tcacgctacg attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc
     1261 attgagattt tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata
     1321 ggataaagat ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat
     1381 tttaatatcc aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc
     1441 atccgccgcg caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc
     1501 tgaatcgtca gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca
     1561 cggcacactt tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa
     1621 tccacaaatt cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac
     1681 ccctgacgta ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg
     1741 ccaaagcgac gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg
     1801 aacatcgctg ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc
     1861 caaccgtgcg gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc
     1921 tccagccctt gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca
     1981 gccatcattg ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt
     2041 tcagcttcac tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag
     2101 ctttttccca gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt
     2161 ggcggcgtta aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc
     2221 gggtggcgaa aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc
     2281 atgcttttcc agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact
     2341 tgccccgcac caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta
     2401 attaatcaaa ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat
     2461 cggagccggt cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct
     2521 ggtttatcca tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa
     2581 gcgcgatacg gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt
     2641 gcatataacg cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc
     2701 cactgcgttt ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg
     2761 gcatggcgaa atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac
     2821 gcgggttaaa gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt
     2881 tcgccagacc agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat
     2941 aatctttacc ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat
     3001 caagcatttc tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat
     3061 ccatttgcca ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt
     3121 ttctggcgct ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact
     3181 ctccttgctg catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc
     3241 gggtttcacg acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg
     3301 tccaggagag ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt
     3361 gggtgatatc acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag
     3421 tggaaacaac atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct
     3481 ggggaatggc ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga
     3541 gcgaacgaat ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt
     3601 gataaacata gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca
     3661 taacgcggtg tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt
     3721 gcacgtaatc acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt
     3781 gactcaggaa ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca
     3841 acgttcccat acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact
     3901 gtggctcagc aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta
     3961 aacgctgcgc gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc
     4021 cgaactgttt ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga
     4081 gcggcgcgag ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct
     4141 tcaggttggc gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga
     4201 ttgcaaccgc tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc
     4261 cgtgatggtg atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa
     4321 tacttttcat cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc
     4381 ctggtaattt gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga
     4441 ctaagtccgc gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg
     4501 aagcgccgtc aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga
     4561 tgaacccaac gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc
     4621 cccagagagc ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac
     4681 caatcatcgt aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac
     4741 taaaattaac atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg
     4801 ccgcgtcctc tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg
     4861 gccaaatggc gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga
     4921 tgaaggggtt atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta
     4981 tctcgacacc acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca
     5041 taaagaagat attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc
     5101 gatgcaaaag ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa
     5161 tcgcccgcaa ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt
     5221 ggcgttatat gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt
     5281 ttctcacgat ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca
     5341 catttgttgt tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt
     5401 tggtcctcgt ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga
     5461 tttacaggga cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt
     5521 cccggttggt tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta
     5581 gtctggcgtc gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc
     5641 gcgtttggtt tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg
     5701 ctggcgggcg gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta
     5761 gggattatgg cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat
     5821 attcgtgttg atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat
     5881 ctcatctcta ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc
     5941 aatttgctgt cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag
     6001 cgcgtgaaat tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt
     6061 gtcggtgcgt tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt
     6121 gcccgtacgc cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact
     6181 ggcggtttaa ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt
     6241 gctgcactgt tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa
     6301 cacatttgtc ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag
     6361 gccggataag acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga
     6421 gcgaccaatc cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc
     6481 gtcgtcgcca tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc
     6541 tccagcacat cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga
     6601 cctaccggcc caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa
     6661 tcgaaacctt cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag
     6721 atggtgccat cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca
     6781 atacgtggcg taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca
     6841 agccccataa agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac
     6901 tccagacgtt gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc
     6961 gtggttgcac caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga
     7021 ccttcaccaa tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca
     7081 acaactggcg atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta
     7141 gtgagcatcg ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa
     7201 ttaacgccca tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga
     7261 ccaaaaatca acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc
     7321 tccatctgtg aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg
     7381 gcgcgatctg ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca
     7441 atcatccttt acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg
     7501 cgtcagtgcg agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg
     7561 ccaccagcgc ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg
     7621 tgagcaccag atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt
     7681 tcatttcgac aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg
     7741 ctcccacttc ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga
     7801 tcgccagcgc caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc
     7861 gctcttgttt attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg
     7921 tgaaaacgat cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat
     7981 gcacttcata gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga
     8041 tgatgcctct gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct
     8101 gtatgataaa aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt
     8161 ctcgatgaac tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat
     8221 attttaatat tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg
     8281 aaaaaaaata aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa
     8341 cgtgaatatt aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg
     8401 tgctgcctta tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat
     8461 cattatcggt cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac
     8521 catcaccacg ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg
     8581 attagacgct gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg
     8641 gctacgcaaa ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa
     8701 atttgttttt gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt
     8761 tggagaactg ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt
     8821 aggttctatt ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg
     8881 tctggtgttg attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt
     8941 ataacttaac gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca
     9001 ttctggctga cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt
     9061 ggattagcgg gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca
     9121 ctaccaatac ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat
     9181 tcctgattca ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag
     9241 tcggcatttt tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg
     9301 atgatttccg tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg
     9361 gtgcgtatgc atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca
     9421 tagccgatca cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt
     9481 tttgctgttt agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt
     9541 atggtagact tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt
     9601 cggtgcggtt tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct
     9661 gtctgctttc agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc
     9721 agtgtagacg tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag
     9781 tgctgcttcc ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct
     9841 gaacagatag gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg
     9901 acgcacttca gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt
     9961 gccgccagga ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc
    10021 cacaccacgt gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt
    10081 atccaccgcc gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc
    10141 ggttaccagc tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc
    10201 tttacgatgt ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag
    10261 ccctgcctgt tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg
    10321 acttagtgag cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga
    10381 agactacctg gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca
    10441 cgcccggcgc atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac
    10501 agtcaattaa ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc
    10561 gcatggcagc ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga
    10621 aatcgggatc gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga
    10681 ccactaagat cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac
    10741 tgccttcgct catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg
    10801 ccttgatgca acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct
    10861 tcacaacctg aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag
    10921 tcatcagaca cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg
    10981 tgccagtcag cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag
    11041 taccgtattt cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga
    11101 tacccagaat accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac
    11161 cgcccacttc gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg
    11221 cagctttcag ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg
    11281 tcaggccgcc ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc
    11341 atttgctttc gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg
    11401 cgtcggcaac aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg
    11461 cagtacgatc caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt
    11521 tgataccttc cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc
    11581 cgtagatttt aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac
    11641 ccggaacacg tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg
    11701 caaactcaac agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat
    11761 ccggtttatc agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc
    11821 ccagatccac gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac
    11881 gcacttgcgg cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac
    11941 gatcagcgcg caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac
    12001 cggacatcat cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac
    12061 ctttgtgcac acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca
    12121 gcatcggagt ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg
    12181 tgattttagc gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc
    12241 ggtatttcag acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg
    12301 cgcggttgat gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc
    12361 ggttaatatt tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat
    12421 tacgcagttc agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca
    12481 cgataccttc gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt
    12541 tgacccaacc acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat
    12601 attctgtacg catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag
    12661 gtgaccaagt cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata
    12721 ctggaaattc tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt
    12781 atatcgggcc tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat
    12841 aaaaattaac aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga
    12901 ttttgttttt tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt
    12961 gatcgattta caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt
    13021 agttaatcgc gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct
    13081 ggtgcgagtt ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc
    13141 ctcccccgct aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac
    13201 tgacagtgat atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga
    13261 gttgcaactg cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat
    13321 cggtgttgaa tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga
    13381 agacgcctgt agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc
    13441 gcgcatcgcc cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt
    13501 ctaccgcaat ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat
    13561 gcccgccact ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag
    13621 catgtgtttt ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa
    13681 agttcatatg taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt
    13741 gccccatgca tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag
    13801 ttaaaaataa tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg
    13861 catagccaaa gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga
    13921 attaacccga cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag
    13981 caacgatggc ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca
    14041 ggaaactctg aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg
    14101 taaaatcagt aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag
    14161 ccgcagcacg ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat
    14221 catgattggg cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa
    14281 acaccgacca gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa
    14341 taaaaaaccg ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat
    14401 accagtggca ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa
    14461 attgccccct cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag
    14521 tgtaaagcag aagttcttca atatataaca tatggagtta atatgcttac acaaactata
    14581 tattcgcctc ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc
    14641 agataaaaaa gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc
    14701 gacaaataac aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag
    14761 ccgtgcatat gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga
    14821 agaacaacga gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa
    14881 cctggacttg tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc
    14941 aattaatata agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca
    15001 gcttaatgtc agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt
    15061 attgaatgcg tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga
    15121 gcttcatgtc tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac
    15181 attagatgta tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga
    15241 gattagcgca atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc
    15301 catatgggca atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa
    15361 cggttatttt gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa
    15421 tgaatgttca atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca
    15481 aagattaacc tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg
    15541 acaacagaat acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa
    15601 caaacaatct gatgtatcac agatatggca tgcttttgaa c
//
LOCUS       DAJTVE010000096        15585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000096 DAJTVE010000000
VERSION     DAJTVE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..418)
                     /locus_tag="OO439_002902"
     CDS             complement(89..418)
                     /locus_tag="OO439_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6317217.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(423..608)
                     /locus_tag="OO439_002903"
     CDS             complement(423..608)
                     /locus_tag="OO439_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6317218.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(605..3244)
                     /locus_tag="OO439_002904"
     CDS             complement(605..3244)
                     /locus_tag="OO439_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6317219.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3452..4441
                     /gene="ldhA"
                     /locus_tag="OO439_002905"
     CDS             3452..4441
                     /gene="ldhA"
                     /locus_tag="OO439_002905"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6317220.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4552..4974
                     /gene="hslJ"
                     /locus_tag="OO439_002906"
     CDS             4552..4974
                     /gene="hslJ"
                     /locus_tag="OO439_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6317221.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4971..5237)
                     /locus_tag="OO439_002907"
     CDS             complement(4971..5237)
                     /locus_tag="OO439_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6317222.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5511..9035
                     /gene="nifJ"
                     /locus_tag="OO439_002908"
     CDS             5511..9035
                     /gene="nifJ"
                     /locus_tag="OO439_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6317223.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9401..10534
                     /gene="ompN"
                     /locus_tag="OO439_002909"
     CDS             9401..10534
                     /gene="ompN"
                     /locus_tag="OO439_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6317224.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10675..11109
                     /gene="uspF"
                     /locus_tag="OO439_002910"
     CDS             10675..11109
                     /gene="uspF"
                     /locus_tag="OO439_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6317225.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11695..12609
                     /gene="sitA"
                     /locus_tag="OO439_002911"
     CDS             11695..12609
                     /gene="sitA"
                     /locus_tag="OO439_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6317226.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12609..13436
                     /gene="sitB"
                     /locus_tag="OO439_002912"
     CDS             12609..13436
                     /gene="sitB"
                     /locus_tag="OO439_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6317227.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13433..14290
                     /gene="sitC"
                     /locus_tag="OO439_002913"
     CDS             13433..14290
                     /gene="sitC"
                     /locus_tag="OO439_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6317228.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14287..15144
                     /gene="sitD"
                     /locus_tag="OO439_002914"
     CDS             14287..15144
                     /gene="sitD"
                     /locus_tag="OO439_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6317229.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15345..>15585)
                     /locus_tag="OO439_002915"
     CDS             complement(15345..>15585)
                     /locus_tag="OO439_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6317230.1"
                     /translation="RLYPVVMAAKSDIVRDVMTWGDADNQQVKQSMPELEELAAAMAQ
                     AQVERNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
BASE COUNT         3901 a         3944 c         3748 g         3992 t
ORIGIN      
        1 acaggagttt ttgaatattc accttgtttc aggaaaatag atcaatcgct ttctcctgta
       61 gcgttatccc gatattgcgg ttttgctatt aaaattttcg cacccattta ccgtttatcc
      121 cttgtacata ttcccccggt ttggcccgag ctacgagctt ttgcccggcg agtttcgcga
      181 tatcatctac cgacacatta ttttgctttg ccagttgttg gtaactcgct ttacgttcgg
      241 cattaatgtc ggttactaat ttctctgttt cagcatccgt tttcagcgca accagataac
      301 cataaaatgt ttcacctacc cgcccctgag ttctggcttc atccagagtc aatgccatta
      361 cattgctgct taccagccca acaagaaacg cacaaagaag taatgttttc ttcatcatcg
      421 cctcagaaaa gatcgctacg agtttcaagc agctcttcga catctttgtc tgccttgatg
      481 atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt gggcgcagcg
      541 acttcaatac gcggcgtaca gccaaccagc ataaaagatg acgtcaacgc agccagtaaa
      601 attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc ggcagacgtg
      661 cgttctgctc caaccatgcc tggagattat cgccaaagcg taaactgcgc cacagcgtaa
      721 aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca tcaacgcgac
      781 tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatctaag ttaatttttg
      841 tcgatgaccg gctaatttcc atatagcgca accagttaat tgctgaaccc gcagtcatat
      901 tgtctttcac caccgcatct gcggtgtctt tatccagccg caaggtcatt ggcccgctat
      961 ttgccagcca accatctttc actatccatt tttcgttgtt tagccataac ggcaatgcac
     1021 cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc agttcgctgg
     1081 atgagagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt aattgtttca
     1141 tcagtacgtt accgcctaac acatcgacgc taacatcagt caacagcaag ggttcttctt
     1201 cagtccacgg ataacgccct tgcaaatcag ccgtaatatt tttcgctgtc accagattaa
     1261 tcacttcggc aattcgcaac gtaacggggc cgcgagtccc cagatgccag gctccatcgg
     1321 caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc atccaggcac
     1381 taccgccttt caacacgccg tgtcctcccg cgcggaatcc ttgttcaggc gcagcagaaa
     1441 atgcaacctg agcatacagt tccccatcgc gtaagttcat cttccagtcg ggtggcacca
     1501 gcggctggaa tacagtcagt gattgttttg gccaccaggc gttaccgcgc agacgaatac
     1561 cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa
     1621 agaggaaata cgtcggatcg cgcccatcga cgctaaattt taaggttgat ggcggtaaca
     1681 cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagcgcgccg ctgaaggagg
     1741 gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag aattaatcgc ggcttttcta
     1801 ccgtcatcgt accgtattgt aactggtcga aaccggtgga gaggtcggtc agcgtaatcg
     1861 tgctgtcatg ccattcacct tttcctgcga catcccaggt ggcatttatc ggtgtaaaac
     1921 tgccttttcc ccagtagcgc cactgccagc ggcccgcgtc agggagaaaa tcattcgcca
     1981 gcccatccat atgcagcacg aaatcgccca gttcatattc atgcgcctgc aaaatggcct
     2041 gcaaacgtcc gtcaacacca cgttgggtga cttttacacc cgctaaaggc cagcggattt
     2101 catcgatgtc cagcgaatcg atgactcttc ccttcgaacg taataacgcg cctggctcaa
     2161 aggccagcgt tgggtcagtc agacttccac ttagctgcgc gggtaagcgg gcatataaaa
     2221 tgaggtccgc ctgtttcgct tcaccggtca gctgcagagg cagctggctg ttatccatgc
     2281 ttaattttcc tgggccaaaa tttagcaccg cgttgccttt acccgcttgc ccctgggtca
     2341 gcacactcag tcgtccgctg atcagagcgt tctcaagccc tgcctgccag ttgtcgactt
     2401 tgacacccag tcggccactc aaaggaaaac ctgcatacgg ccaactccag cgaccatcgc
     2461 ttacggtcaa ttgttgacga gtaatttgcc acggcaaatc gagcaacgga tcgccgttat
     2521 cccgtgccag cacaatcaat tgcccgctat tttcctgcca gtccagctct gcatccacca
     2581 gtgacggttc ctgcggcaag tttaacgtcg cagtagcatg cccacttaca ggaagtccat
     2641 ccggcacgag cggcatagta aattccccca ccagtttaac cggcggctga ttttcaaacg
     2701 cgatgacatc cagttcgctg actgtaagtt gttgcccttt cagctggcct tgaaatttaa
     2761 ctttttcgcc ctgataacgc agttgctgaa tatcagaggt taatgcgaga gagagttttc
     2821 cctgccattc ctgccaggga gaaaaaatca gtttatcgat attgatccag gtgttaggca
     2881 gcatggactg ccactgcgcg agggtttttg gagcagctgg cgattgctcc gtctgcggca
     2941 atttcgccag acaagcagaa tcaagttcta ccatgccgac gttcaataac cagcggctgg
     3001 gatgtgaaag gctggcgttg gtgatatgcg caagctgaca atctcccacc agataacgga
     3061 gatcgggaat gattaaacct ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg
     3121 tcccgagcgg tagccagatg cccgccagcg tgggaaccca cagcccgagc gtcatcagca
     3181 gcgtcaacgg cacaagaata atcagtaata acagcgcgag aacggcttta tatttaccca
     3241 gcatgggtag ttaatatcct gatttagcga aaaattaagc attcaatacg ggtattgtgg
     3301 catgtttaac cgttcagttg aaggttgcgc ctacactaag cattgttgtt gatgaatttt
     3361 tcaatatcgc catagctttc aattatattt gaaattttgt aaaatatttt tagtagctta
     3421 aatgtgattc aacatcactg gagaaagtct tatgaaactc gccgtttata gcacaaaaca
     3481 gtacgacaag aagtacctgc aacaggtgaa cgagtccttt ggctttgagc tggaattttt
     3541 tgactttctg ctgacggaaa aaaccgctaa aactgccaat ggctgcgaag cggtatgtat
     3601 tttcgtaaac gatgacggca gccgcccggt gctggaagag ctgaaaaagc acggcgttaa
     3661 atatatcgcc ctgcgctgtg ccggtttcaa taacgtcgac cttgacgcgg caaaagaact
     3721 ggggctgaaa gtagtccgtg ttccagccta tgatccagag gccgttgctg aacacgccat
     3781 cggtatgatg atgacgctga accgccgtat tcaccgcgcg tatcagcgta cccgtgacgc
     3841 taacttctct ctggaaggtc tgaccggctt tactatgtat ggcaaaacgg caggcgttat
     3901 cggtaccggt aaaatcggtg tggcgatgct gcgcattctg aaaggttttg gtatgcgtct
     3961 gctggcgttc gatccgtatc caagtgcagc ggcgctggaa ctcggtgtgg agtatgtcga
     4021 tctgccaacc ctgttctctg aatcagacgt tatctctctg cactgcccgc tgacaccgga
     4081 aaactaccat ctgttgaacg aagccgcctt cgatcagatg aaaaatggcg tgatgatcgt
     4141 caataccagt cgcggtgcat tgattgattc tcaggcagca attgaagcgc tgaaaaatca
     4201 gaaaattggg tcgttgggta tggacgtcta tgagaacgaa cgcgatctat tctttgaaga
     4261 taaatccaac gacgtgatcc aggatgacgt attccgtcgc ctgtctgcct gccacaacgt
     4321 gctgtttacc gggcaccagg cattcctgac agcagaagct ctgaccagta tttctcagac
     4381 tacgctgcaa aacttaagca atctggaaaa aggcgaaacc tgcccgaacg aactggttta
     4441 atcttgccgc tcccctgcat tccaggggag cttattcaga taatccccaa agacctttca
     4501 tcttctattc ttaaaatagc cctgagtcag aaactgtaat tgagaaccac aatgaagaaa
     4561 gtagccgcgt ttgttgcgct aagcctgctg atggcgggat gtgtaagtaa tgacaaaatt
     4621 gctgttacgc cagaacagct acagcatcat cgctttgtgc tggaaagcgt aaacggtaag
     4681 cccgtgacca gcgataaaaa tccgccagaa atcagctttg gtgaaaaaat gatgatttcc
     4741 ggcagcatgt gtaaccgctt tagcggtgaa ggcaaactgt ctaatggtga actgacagcc
     4801 aaagggctgg caatgacccg tatgatgtgc gctaacccgc agcttaatga actcgataac
     4861 accattagcg aaatgctgaa agaaggtgca caagtggatc tgaccgcgaa ccagttaacg
     4921 ctggcgaccg cgaaacagac attaacttat aagctggcgg atttaatgaa ttaatagctg
     4981 ccacagctcc cggcggcaag tgactgttcg ctacagcgtt tgccgttggg taatgcacac
     5041 atcccaatcg ccgtaccatc cagttgacgg gcaacagaaa gcgaaccgcc gatcattgca
     5101 caatttgctt ctccactact ggacatcgac gcttttaaac ctggcgctac gtgcgccgca
     5161 gtcgcctgct gaacaggttc actactacac gccgacaaca ataaagcggc acaccctacc
     5221 caaaacgctg ctcgcatctc ttcttcctct gatcttcaag ccaaacgaca ccgccataaa
     5281 taataggcag cacagagggc ggcgtcgaga gctgtcctgc gcgttgcccc gcatttttac
     5341 ttttttatgg ctattttttt gccctctgtt tgatcaaaac attcattacg ctgatgtggg
     5401 ggacacaaaa gcgaaaatgc agaagaaagc catttgctaa aattgaagga ttactactgg
     5461 gcgcgcagca atttcgtgcg cccctcattt gcgcaatgta agggtgtcat atgattacta
     5521 ttgatggtaa tggcgcggtt gcttcggtgg cgtttcgcac cagtgaagtt atcgccatct
     5581 accctattac ccccagttcc acgatggcag aacaggctga tgcctgggcc ggaaacggct
     5641 tgaagaacgt ttggggagac acaccatgcg tggttgaaat gcagtcggaa gcgggtgcta
     5701 tcgctaccgt gcatggcgct ttgcagacgg gtgccctttc aacatcgttt acgtcatcgc
     5761 agggtttgct gctgatgatc ccgacactgt acaaactggc aggcgaactc acaccgtttg
     5821 tcctgcatgt agcggcacgt accgttgcca cacatgcact ctctattttt ggcgatcatt
     5881 ccgacgtcat ggcggtgcgc cagacgggtt gcgcgatgtt gtgtgcggca aacgtccagg
     5941 aagcgcaaga ctttgctctc atttcgcaca tcgcgacgct gaaaagccgc gtgccattta
     6001 ttcatttctt tgatggtttc cgcacgtccc acgaaatcaa caaaattgtc ccgctggccg
     6061 atgacacgat tcttgatctc atgccgcagg ccgaaattga tgctcatcgc gcccgggcgc
     6121 tcaacccgga acatccggtg atccgtggta cgtccgccaa tcctgacact tatttccagt
     6181 ctcgcgaagc caccaacccc tggtacaacg cagtctatga ccatgttgaa caggcgatga
     6241 atgatttctc tgccgcgaca ggtcgtcagt atcagccgtt tgaatattac gggcatccgc
     6301 aagctgaacg ggtgattatc ctgatgggct cagccattgg cacctgtgaa gaagtggttg
     6361 atgaattgct aacccgtggc gaaaaagtcg gcgtgctgaa agttcgcctg taccgcccct
     6421 tctcagccaa acacttgcta caaactctac cgggatccgt acgcagcgtg gcggtactgg
     6481 acagaaccaa agaacccggt gcccaggcag aaccgctcta tctggatgtg atgaccgcac
     6541 tggcagaagc ctttaataat ggcaagcgcg aaactctgcc ccgtgtcatt ggtgggcgct
     6601 atggtctttc atccaaagaa tttggcccgg actgtgtact ggcggtattt gccgagctca
     6661 acgcggctaa accgaaagcg cgctttacgg ttggtattta cgatgatgta accaatctgt
     6721 ccctgccgtt gccggaaaac acccagccaa actcggcgaa acttgaagcc ttgttttatg
     6781 gccttggtag tgatggcagc gtttccgcga ccaaaaacaa tatcaagatt atcggtaatt
     6841 ccacgccgtg gtacgcacag ggctattttg tttacgactc caaaaaagcg ggcggcctga
     6901 cggtttctca ccttcgcgtg agcgaacagc cgattcgttc cgcttatctc atttcccagg
     6961 ctgattttgt tggctgccac cagttgcagt ttatcgataa atatcagatg gctgagcgtt
     7021 taaaacctgg cggcattttc ctgctcaaca cgccgtacag cgcagatgaa gtgtggtcgc
     7081 gcttgccgca agaagttcag gccgtgttaa accagaaaaa agcgcgcttc tatgtgatta
     7141 acgcggcgaa aatcgcccgc aaatgtggcc tggcggcccg tattaatacc gtcatgcaga
     7201 tggctttttt ccatctgacg caaattctgc ctggcgatag cgccctcgca gaattgcagg
     7261 gtgcgattgc caaaagttac agtagcaaag gccaggatct ggtggaacgc aactggcagg
     7321 ctctggcgct ggcgcgtgaa tccgtagaag aagttccgtt gcaaccggta aatccgcaca
     7381 gcgccaatcg accgccagtg gtttccgatg ccgcccctga tttcgtgaaa accgtaaccg
     7441 cagcgatgct cgcagggctt ggtgatgccc tccccgtttc ggcgctgccg ccagacggca
     7501 cctggccgat gggcaccact cgctgggaaa aacgcaatat cgccgaagag atccccatct
     7561 ggaaagagga actctgtacc caatgtaacc actgcgtcgc agcttgccca cactcagcta
     7621 ttcgcgcaaa agtggtacca cccgaagcga tggaaaacgc ccctgccagc ctgcattcgc
     7681 tggatgtgaa atcgcgtgat atgcgcgggc agaaatatgt cttgcaggtg gctccggaag
     7741 attgcaccgg ttgtaacctg tgcgtcgaag tttgcccggc gaaagaccgt cagaatccag
     7801 agattaaagc catcaatatg atgtcgcgcc tggaacatgt cgaagaagag aaaatcaatt
     7861 acgatttctt cctcaacctg ccagaaatcg accgtagcaa actggaacgt attgatattc
     7921 gtacatcgca gctgattaca ccgctgtttg aatattccgg tgcttgctcc ggttgtggcg
     7981 agacgccgta tattaaattg ctgactcagc tctatggcga ccggatgttg atcgctaacg
     8041 ccaccggctg ttcttcaatt tatggcggta acctgccctc tacaccgtat accaccgatg
     8101 ccaacggtcg tgggccggca tgggcgaact ctctatttga agataatgcc gaatttggcc
     8161 ttggtttccg cctgacggtc gatcaacacc gtgtccgcgt gctgcgtctg ctggatcaat
     8221 ttgccgataa aatcccggcg gaattactga cggcgttgaa atcagacgct acgccagagg
     8281 ttcgtcgaga acaggtcgca gctttacgcc agcaactcaa cgatgttgcc gaagcacatg
     8341 aactgctacg tgatgcagat gcactggtgg aaaaatcaat ctggctgatt ggtggtgatg
     8401 gctgggctta cgatatcggc tttggcggtc tggatcatgt attgagtttg acggaaaacg
     8461 tcaacattct ggtgctggat acgcaatgct attccaacac cggtggtcag gcgtcgaaag
     8521 cgacaccgct gggtgcagta actaaatttg gcgagcacgg caaacgtaaa gcgcgtaaag
     8581 atcttggcgt cagtatgatg atgtacggtc atgtttatgt ggcgcagatt tctctcggcg
     8641 cgcagctgaa ccagacggtg aaagcgattc aggaagcgga agcgtatccg ggaccatcgc
     8701 tgatcattgc ttatagcccg tgtgaagagc atggttacga tctggcactc agccatgacc
     8761 agatgcgcca actcacagct accggcttct ggccgctata tcgcttcgat cctcgtcgtg
     8821 ccgatgaagg caaactgccg ctggcgctgg attcacgccc gccgtcagaa gcactggaag
     8881 aaacgttact tcacgagcaa cgtttccgtc gtctgaattc gcagcagcca gaagtggcag
     8941 aacagttatg gaaagatgct gcagctgatt tgcaaaaacg ctatgacttc ctggcacaaa
     9001 tggccggaaa agcggaaaaa agcaacaccg attaaatgct ctggataagg attacccaat
     9061 tctaaaaaaa agcccggacg actgttcggg cttgtctttt tatatgttgg aaaatcagtg
     9121 gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat aaaggcatat aacccgcgca
     9181 gaataacgta tagaaaatta aaagtataat ttttattttg tatgtattat cttcgataca
     9241 ttcaccagac ttattatcat ttcagaagaa ttatttgatt aaggttttac ttaaggcgta
     9301 acaaatgata tttatcggct aactgaactt ctcctttagg atgttttcac tccccggaat
     9361 ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagca aagtactggc
     9421 acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag tttataataa
     9481 agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa
     9541 ttcagcgaaa gatggcgatc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat
     9601 taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa acaacactga
     9661 atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc gggctgaaat ttgcagatta
     9721 cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag gctggaccga
     9781 tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta tgactggtcg
     9841 tgccaacggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa atggtctgaa
     9901 cttcgcggtg cagtatcaag gtaacaacga aggtgccagt aatggtcagg aaggcaccaa
     9961 caacggacgt gatgttcgcc atgaaaatgg tgacggctgg ggtctttcca caacatatga
    10021 tttaggcatg ggctttagcg ctggtgcggc atacacctca tctgaccgca ccaatgacca
    10081 ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg ggctaaaata
    10141 cgatgctaac aatatttacc tggcaaccat gtattcagaa acgcgtaata tgaccccgtt
    10201 tggcgacagc gattatgctg ttgcaaacaa aacccagaac tttgaagtca ctgcacagta
    10261 ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
    10321 gcacgctgcc gatggtgcag acaacccggc aggtgttgat gataaagatc ttgttaaata
    10381 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacttatg ttgactataa
    10441 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
    10501 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
    10561 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
    10621 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
    10681 agaacgattc ttgtccctat cgatatttcc gattcagaat taactcaacg cgtgattagc
    10741 cacgttgagg cagaggcaaa gattgacgat gcagaggttc atttcctgac ggtaatacct
    10801 tcactgcctt actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
    10861 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
    10921 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
    10981 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
    11041 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
    11101 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
    11161 ctgacatttt cccctctaat ccgtaccata cacgccacag ttttatatca gatttcttat
    11221 gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
    11281 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caagtaaggg
    11341 caaaaatcac aactatctga cccataagta ttttatttat ttttcaatat gttaaacatt
    11401 atcagcgaca acaataagct acgataaccc actctttttt tgcccctttt ttgccccttc
    11461 tacagcgttt tgccccattt ttgccatcaa caattttcct cataaagtca tttcaaagcc
    11521 taatagctgc tctacttttt tcctcaatat catgtcgcca gcaggaaaca acctaatccc
    11581 tgttttaggc ctcaaagctg ttaatgataa tcattttcat tttgtatggg ttatgctata
    11641 aaacatagcc gttgctatgt ttgttctaat tttgctcact ataggtacta aattatgcac
    11701 tcaataaaaa aagtaaccat gctcttgggg tgcctcgccc tcacctgctc gatcgcattt
    11761 caggcaagtg caactgaaaa attcaaggtc attacaacat ttaccatcat cgcggatatg
    11821 gccaaaaacg tggctggaga tgctgcagaa gtctcatcca taaccaagcc tggtgcagaa
    11881 attcatgagt atcagcctac ccctggcgat attaaacgtg cgcagggggc acaactgatt
    11941 ctcgccaatg gtatgaatct ggaattgtgg ttccaacgct tttaccagca tctcaatggg
    12001 gttccagaag taattgtctc ttcgggtgtg acgccagtag ggatcaccga aggaccctat
    12061 gagggcaaac ctaatcccca tgcatggatg tcgccagata atgctctgat ttacgtcgat
    12121 aatattcgtg atgcgttgat aaaatacgat ccggcaaatg cacaaaccta ccaacgtaat
    12181 gccgatactt ataaagccaa gattacccaa acccttgccc ccctgcgtaa gcagattacg
    12241 gaactccctg agaatcagcg atggatggtc accagtgaag gggctttttc ttatctcgca
    12301 cgcgatttgg ggctaaaaga gctttatctg tggccgatta atgccgatca acaaggaaca
    12361 ccgcagcagg tacgtaaggt tgttgatata gttaagaaaa atcatatccc ggcagtcttt
    12421 agcgagagta cgatttccga taaaccagcg cgtcaggttg cgcgtgaaac cggcgcacac
    12481 tacggcggtg tgctctatgt cgattccctg agcacagaaa acggcccggt acctacgtat
    12541 atcgaccttc ttaaggttac taccagtacg ctggtacagg gaattaaagc cgggaaaagg
    12601 gagaaataat gatgcaatct gcaggcattg tcgttaatga tgttactgtc acctggcgta
    12661 acgggcacac agcactgcgc gatgcatcct tcacagtacc aagtggatct attgccgctc
    12721 tggttggggt aaacggttcc ggaaaatcga cgttgtttaa agcgattatg ggatttgtgc
    12781 gtctgaccag cggaaagata tctgttctgg gtattcccac gcgacaggcg ctacagaaaa
    12841 acctggttgc ctacgtccct cagtcagaag aggttgactg gtcatttcct gtactggtgg
    12901 aagatgtggt gatgatgggc cgctatgggc atatgggtat gttgcgtata gccaaaaaga
    12961 gggatcgtca gattgtcacc gatgcgctgg aacgtgtcga tatggtggat tttcgccatc
    13021 gtcaaatcgg cgagctttcc ggtggacaaa agaaacgcgt ctttctggcg agagcgattg
    13081 cacagcaggg tgatgtgatc ctgctggatg aaccttttac cggcgttgat gtgaaaaccg
    13141 aagcgaaaat catcagcctg ttgagggagt tacgcgcgga aggcaaaacg atgcttgtct
    13201 caacgcacaa tctcggctca gtcacgacat tttgcgatta tacggtcatg gtcaaaggca
    13261 ccgtcctggc aagcgggccg acagacacca cttttacagc cgagaacctg gagctggctt
    13321 ttagcggcgt actgcgccat gtcacactta acgactcgga agaaagcatt attactgacg
    13381 atgaacgccc ttttgtggca catcgaccgt cagcggtgca gagggaagca agatgaacgt
    13441 gctactggaa cccttcagct atgaatatat gctcaatgcg atgtgggtct cggcaatggt
    13501 cggcggtctc tgcgctttcc tgtcatgcta tctgatgcta aaaggctggt cgctgattgg
    13561 cgatgcgctg tcgcactcta ttgtcccggg tgttgcgggg gcatatatgc tggggcttcc
    13621 gttttctctg ggagccttct tttccggcgg actggcagcg ggcagcatgc tgtttcttaa
    13681 ccaacgcacc cgcttaaaag aagatgccat catcggcctg atcttctcgt ccttttttgg
    13741 cctggggctg tttatggtgt cattgaaccc gacatccgtg aacattcaga ccatcgtcct
    13801 gggcaatatt ctggcaatcg atccggcgga tatcctgcaa ctcactatca ttggcattct
    13861 ctcgatcatc gtgctttttt tcaaatggaa agatcttatg gtgacctttt tcgacgagaa
    13921 tcacgcccgt gctattggat tacatccggg cagattaaaa atcctcttct tcaccctgct
    13981 gtcggtttcg accgttgcgg cgctgcaaac ggttggtgcc tttctggtga tctgtctggt
    14041 ggtgactccc ggtgcaacag catggttgtt aaccgatcgt tttccccgcc tgctcataat
    14101 tgctgtaact attggcagtg taaccagttt cctcggcgcg tgggtcagtt actttctgga
    14161 cggcgcgaca ggtggaatca tcgtggtggc gcagacactg ctgtttcttt tagcatttgt
    14221 atttgcgccc acccatggac ttttggcaaa tcgtcggcgt gcgcataaag cgctggagga
    14281 tcgctcatga tggcattatt actggagcca ctgcagttca cctttatgag tcacgcactg
    14341 ctgatttcac tggtagtttc cataccctgt gcgctgctgt cggtctttct ggtcctgaaa
    14401 ggttgggcac tgatgggtga cgcgatgagt catgcggttt ttcctggcat cgtgcttacc
    14461 tggattctgg ggttacctct ggctacaggg gcattcgtcg ccggagtatt ctgcgcggtg
    14521 gcgacgggat acctgaaaga caacagccga atcaagcaag atacagttat ggggattgtt
    14581 ttttctggca tgtttgccgc aggtctgatc ttgtatattg caatcaaacc agacgtacat
    14641 cttgaccata ttcttttcgg cgacatgctc gggataacca tcggcgatat aatccagacg
    14701 gtgattattg ccgggctggt tacacttgtt attagcgcaa aatggcggga ttttttgctg
    14761 ttcagctttg attatcaaca ggcgcaggta agcggtttgc atacgagatg gctgcattat
    14821 gggctgctgt gcatggtttc cctgaccatt gtggcgacgc tgaaagcagt aggtatcatt
    14881 ctctctattt ctctgctcat tgcgcctggc gccattgcag tactcctgac acaacgcttt
    14941 catattgcgc tcctactggc gactggcata tcggtaatag tgtcaatgac tggtgtctgg
    15001 ctgtcctttt ttatcgacag cgcaccagct ccgacgattg tcgtcttatt cgcggtcgtg
    15061 ttcatcatga cgtttaccgt taccagcatc aacgcacgca caaagggaaa cgcccataca
    15121 caggatctgt tatcacccaa ttagcacaac atccgaggac agcatcgtcc gccagcctac
    15181 agaagtccgg aaagccatgg aaggtcgttg ggaggaggta cgaattctat catgcaaaaa
    15241 atacgtaaaa tcgataacgc ctagaaatca ttcaatactc gcactatcga aagtttacca
    15301 gccaaccgcg gcacgttcct gcatacgacg tgccgcagct tctgttattc cacgataaaa
    15361 ttcctaaccg aattcaaatc cttcagacta ttcagctctt ccttcagctc ccgctgacgg
    15421 cgataaatct cgtcattgcg ctcaacctgt gcctgagcca ttgctgccgc cagttcttcc
    15481 agttccggca tcgactgttt cacctgctga ttatcggcat caccccacgt catcacgtcc
    15541 cgtacaatgt cggatttcgc agccattacc accggataaa ggcgg
//
LOCUS       DAJTVE010000097        15574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000097 DAJTVE010000000
VERSION     DAJTVE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2056
                     /locus_tag="OO439_002916"
     CDS             <1..2056
                     /locus_tag="OO439_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6317231.1"
                     /translation="PPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSGLNL
                     LGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVRFRG
                     VRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDLQLA
                     GGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSDFVQ
                     AGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDVFDG
                     QSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDIADY
                     YQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRRISY
                     TLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASNNTG
                     LSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAGASV
                     SGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTPYRE
                     NHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPLTFG
                     YEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESRNYI
                     CQ"
     gene            2072..3106
                     /locus_tag="OO439_002917"
     CDS             2072..3106
                     /locus_tag="OO439_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6317232.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3188..3526)
                     /gene="rcnB"
                     /locus_tag="OO439_002918"
     CDS             complement(3188..3526)
                     /gene="rcnB"
                     /locus_tag="OO439_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6317233.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3745..4569)
                     /gene="rcnA"
                     /locus_tag="OO439_002919"
     CDS             complement(3745..4569)
                     /gene="rcnA"
                     /locus_tag="OO439_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6317234.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4690..4962
                     /gene="rcnR"
                     /locus_tag="OO439_002920"
                     /pseudo
     CDS             4690..4962
                     /gene="rcnR"
                     /locus_tag="OO439_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5054..5150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5185..5973
                     /gene="thiM"
                     /locus_tag="OO439_002921"
     CDS             5185..5973
                     /gene="thiM"
                     /locus_tag="OO439_002921"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6317235.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5970..6770
                     /gene="thiD"
                     /locus_tag="OO439_002922"
     CDS             5970..6770
                     /gene="thiD"
                     /locus_tag="OO439_002922"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6317236.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6835..7653
                     /locus_tag="OO439_002923"
     CDS             6835..7653
                     /locus_tag="OO439_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6317237.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7705..8451
                     /locus_tag="OO439_002924"
     CDS             7705..8451
                     /locus_tag="OO439_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6317238.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8425..9390)
                     /locus_tag="OO439_002925"
     CDS             complement(8425..9390)
                     /locus_tag="OO439_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6317239.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9387..10391)
                     /locus_tag="OO439_002926"
     CDS             complement(9387..10391)
                     /locus_tag="OO439_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6317240.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10388..11665)
                     /locus_tag="OO439_002927"
     CDS             complement(10388..11665)
                     /locus_tag="OO439_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6317241.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11922..12974
                     /gene="fbaB"
                     /locus_tag="OO439_002928"
     CDS             11922..12974
                     /gene="fbaB"
                     /locus_tag="OO439_002928"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6317242.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13293..14147
                     /gene="gatY"
                     /locus_tag="OO439_002929"
     CDS             13293..14147
                     /gene="gatY"
                     /locus_tag="OO439_002929"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6317243.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14176..15433
                     /gene="gatZ"
                     /locus_tag="OO439_002930"
                     /pseudo
     CDS             14176..15433
                     /gene="gatZ"
                     /locus_tag="OO439_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15443..>15574
                     /locus_tag="OO439_002931"
     CDS             15443..>15574
                     /locus_tag="OO439_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6317244.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     "
BASE COUNT         4088 a         3727 c         3984 g         3775 t
ORIGIN      
        1 tccaccggaa aactgggagc ggggtattaa tgcgttttat acctcttatt atgtgagtca
       61 gtattacagt gactataaag cgtcgggtaa tagtaagagt acatatgtac gttttaacag
      121 cgggttaaat ttactggggt ggcaactgca ttctgatgcc agtttcagta aaacaaataa
      181 caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg cccaacttct
      241 cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt ctgttcgctt
      301 cagaggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac aaaattttac
      361 gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac agaatggttt
      421 tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt tgcagttggc
      481 cggtggtgga gcagatcttg atgtcagcgt gaaagaggcg gacggttcgg taaccaccta
      541 tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga aatatgattt
      601 tgcggcgggt cgtagccata ttgaaggggc gagcaaacat agtgattttg tccaggcggg
      661 ttatcagtat ggttttaata acttattgac gttgtatggt ggctcgatgg ttgcgaataa
      721 ttattacgcg ttcactttgg ggactggctg gaatacacgc attggtgcca tttccgtcga
      781 tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc aaagttatca
      841 aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg cggcctggcg
      901 ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca ataaagataa
      961 ttatcgccgt gatgaaaacg atgtctatga cattgccgat tattaccaga atgattttgg
     1021 tcgcaaaaat agcttttccg ccaatatgag ccagtcattg ccagaaggtt gggggtctgt
     1081 gtcattaagt acgttatggc gagattactg ggggcgtagc ggcagtagta aggattatca
     1141 gttgagttat tccaacaacc tgcgacggat aagctatacc ctcgcggcaa gccaggctta
     1201 tgacgagaat catcatgaag agaaacgttt taatattttt atatcgattc cctttgattg
     1261 gggtgatgac gtttcgacgc ctcgtcggca aatatatatg tctaactcaa cgacgtttga
     1321 tgatcagggg tttgcctcaa ataatacggg attatcagga acagtaggga gtcgggatca
     1381 gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga cagctggagc
     1441 gaatttaacc tggaacgcgc cggtggcaac agtgaatggc agttatagtc agtcgagtac
     1501 ttatcgacag gctggagcca gtgtttcagg gggcattgtc gcctggtcgg gtggcgttaa
     1561 tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc
     1621 ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg
     1681 aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg atagcgaagc
     1741 agaattacgt ggcaaccgga aaattgccgc accttatcgc ggcgcggttg tactggttaa
     1801 ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg atgggcaacc
     1861 attaacgttt ggttatgaag tcaatgatat ccacggtcat aatattggcg ttgtcggcca
     1921 gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg tagcaattga
     1981 taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg aaagtagaaa
     2041 ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc tatttatatt
     2101 aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat ctgttcagca
     2161 caatttggtt gttcaggggg attttgcact tactcaaaca caaatagcga catatgagca
     2221 taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga gttcgtcgga
     2281 tattattgtt gggctttata acgataccat aaaattaaat ttacattttg aatggaccaa
     2341 taaaaacaac atcacgttgt caaataatca gaccagtttc accagtggtt attcagttac
     2401 ggtgacacct gcggccagta atgcaaaagt gaatgtttct gcggggggcg gcggttcagt
     2461 gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg ggagtgccgc
     2521 agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg taaatagcta
     2581 cagaaatgca ttggcacaaa atgcaggtgt ctattccttt aatctgacat tgtcatacaa
     2641 cccgataacc acaacctgca aaccggacga tttattaatt actttagaca gtattcccgt
     2701 ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaacaag gggatattat
     2761 tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa tgcaggtgta
     2821 tttatcaagt tctgatttgt tagccggcag taacacgatc ctgaaaggtg cagaaaataa
     2881 tggagtcgga tttatgcttg aaagcaatgg ttctccagtc acatttttaa atatcactga
     2941 taacagcaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac ttacagatac
     3001 aacggtttca attccgataa ctgccagtta ctacgtctac gatacaaaca aagttaaatc
     3061 tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac aagcaagacg
     3121 agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca tgatgagggg
     3181 gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg
     3241 tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg
     3301 cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg
     3361 ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt aactcatact
     3421 tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg
     3481 taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac tctccacttt
     3541 ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata
     3601 ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat
     3661 ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat
     3721 aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat
     3781 atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt
     3841 gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac
     3901 ggtgacaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt
     3961 cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc aggggataag
     4021 gccaccggtt aagccaaata gcaaaatttg ccagttggtg acctctctac catcaaagcg
     4081 tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactcgc catgttcgtg
     4141 atggtgatga tgtccatggt cgtggtgatc ttcgtgatcg tgatgatgat gctcataatc
     4201 atactcgtgc atattctcca gccagttgcg ttcgccgcgc caggtacgcc agaacatcca
     4261 gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt ctgctgattg
     4321 agcagtaaag cgcttgctga tcaccatccc gccaaaggca attaaccaga ccactgcggt
     4381 atgcgaaata gttgctgcca gtccgagcat caccgcttgt ttaatggtgc ctttgatggc
     4441 gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat gaagcgcacc
     4501 aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa
     4561 ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact ccccccagta
     4621 cctgatacta ccccccagta gattaatagt gctatgatat tttcatattc ttgttaacca
     4681 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
     4741 attcagggct aggtcgtggc gctcaagaaa atgctcgacg agccgcatga atgcgctgca
     4801 gttttacaac agattactgc tatccgtggc gcggtaaacg gtctgatgcg ggaagtgatt
     4861 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
     4921 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
     4981 aaactgccgt ttttcctcgt ttacaacgcg tgcgctggac attaccatcc tcctctgcga
     5041 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
     5101 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
     5161 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgggttcagc gcaatctgcg
     5221 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
     5281 gtgcaaacct ttaccgctaa taccttgctg gcgctcggtg catcgccagc gatggttatc
     5341 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
     5401 acactgacgc agccacgcgc tcaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
     5461 caaacaccct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
     5521 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
     5581 gcattagctg gcattgctaa tggtggacgg ggagtggata ccactgacgc cgcagctaac
     5641 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
     5701 gaggtggatt atgttaccga tggccatcgc gccgttggca ttcacggtgg cgatccgtta
     5761 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
     5821 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
     5881 gagcgcgcag tcgccagaag cgaggggcta ggcagttttg ttccacattt ccttgatgcg
     5941 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
     6001 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
     6061 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
     6121 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
     6181 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
     6241 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
     6301 aaagcggcga cccactgctt tcaccttcgg cagttgccac gctacgcagt cgattattgc
     6361 cacaggtttc attaataacg ccaaacttgc ccgaagcagc cgccttgctc gacgcgccac
     6421 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
     6481 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
     6541 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggta
     6601 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
     6661 acaccgtaca ggaggcaaaa agctggcttt catcggcgtt agcccaggcc gacacgctgg
     6721 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
     6781 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
     6841 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
     6901 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
     6961 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgtattcct
     7021 gccagttaca ccattcacgg aatagatgtt tcgcgctggc aggagcggat cgactggcag
     7081 cgtgtggcaa aaatgcgcga taacggtatt cgcttacaat ttgcctttat taaggcgacg
     7141 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaactaag ccgcgaaaat
     7201 ggcttgctgc gcggggcata tcattatttc tccccgtcgg tagcagcacc ggttcaggtg
     7261 cgattatttc tgcaaacggt agatttctca caaggcgatt tccctgcggt actggacgta
     7321 gaagagcgag gaaaattatc ggcaaaagaa ttacgcaaga gggtaagtca gtggctaaaa
     7381 atggtcgaaa aaagtacggg gaaaaagccg attatttact caggagccgt tttttatcac
     7441 acgaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ctatcaacgt
     7501 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
     7561 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggtgga ggagttgcag
     7621 ggattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat actcccacca
     7681 gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat tgcccaactg
     7741 aacgaacgta ttttagcggc ggataacacg ccgctttata ttaagtttgc cgaaacggta
     7801 aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg tgagcgtgat
     7861 ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa
     7921 gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa
     7981 tactcgttga aagaagcgcg aggtttttct cagcaggtgg tgttgcgcgg gaaaaagccc
     8041 gatacgctat gggttaacaa gcgtgtcgtg aaatgtcccg aagaagtcgc gcagcagctg
     8101 gcggtcgaag caggaagtga tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa
     8161 gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc
     8221 gggatttcac tttatgacta tttccgcagc cagcacattt acccacaacg tacgcgttcc
     8281 cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt agatagcaaa
     8341 ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc tattgagtac
     8401 agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta gttcctcgcg
     8461 cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg cattgcccag
     8521 taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc cacctgcatg
     8581 actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa tgcagccaga
     8641 agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca acggcgcggc
     8701 aaatttctcc tgccattgct cgccaagtgt tgttatctct gcggataaag caaaacgttc
     8761 ggcggcaatc tcagcctctt gacgattgag cgacactaaa ggtcgacagg ccatgatccg
     8821 cgccagtaat gcatccggga tatcgccaat acgtgggcca aaatcgataa acggcgtcac
     8881 gtcttgcagc tcttccagcc attccactaa caattcgccg cagggcgagg ccagttgata
     8941 accggaaaaa tagagcaggc tgccaggcgc aacggttaat cgcgccagcc actggcgatt
     9001 ccactgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag
     9061 cgccagacac cagccgttat caccttcggc gttatcgatc aggctgatta agccctcttt
     9121 tgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca aggcattacc
     9181 cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt
     9241 aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat aagcgtcggc
     9301 gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg ttaattcagg
     9361 cagcagcgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg atatttcgcc
     9421 agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagttc cgcctttaac
     9481 gcaggatcga tagcgttaac gccatgcaac gcgccgcaaa ttgccgtcgc catagcaccg
     9541 atggtgtctg tgtcgccgcc aaggttagcg cacaggacgg cgcagcgatt cgggtcggtt
     9601 tgtgctagtt caaccagcgc aatggcgcac ggaacggact caatagtgct ggtacctgcg
     9661 ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt gcgcacaatt
     9721 ttcagcgcaa tctccagccg cgccgccagt gaggcgctga acgtagtgat gcgtttttgc
     9781 tgtgcatgtc gcgcaattga aggcagggaa tcaacaatcg ctgaccagct ttctccgtca
     9841 atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag atcggattta
     9901 tgggtcgggc tggacgccag cgctacatca tcaataaagg aatcaacatc acgcgccgga
     9961 agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggtcacgcc gttgttttcc
    10021 agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct
    10081 agtacgtttt tgttaaaggc gtcgaaacgc agcgcccagt cgagaatatt acgcccaatc
    10141 agatccggat cgatcttgcc ttcacgttcc agtaacgcat ccgccagaca cagcgccatc
    10201 gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt ctcctttggt
    10261 ccaggaagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg tggccaaagc
    10321 tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc gagaatacgt
    10381 tctgttttca tttaacttcc ccttgtgtca acgcaatatc gcgatcatcg accttgatag
    10441 ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa
    10501 tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg tttaccggtt
    10561 cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg agcaaactgc
    10621 cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata tgcacggggg
    10681 cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt acgccgtgaa
    10741 gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgtacta ccgtaaataa
    10801 agaagccata gcggatcgca gcggtgacca gaccaagcaa taataccttt ttgataccaa
    10861 agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac tggccgagcg
    10921 tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg gcaaagatgt
    10981 aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg aggaagtttt
    11041 tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatatcc attttgccgg
    11101 tgctttttgg tggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga gcagaacttc
    11161 cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc agtatttgcg
    11221 gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc acacgaatgc
    11281 gcgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta gtcagcgcaa
    11341 tggtcggcat ataggttaga gagtaagcca gcagtaacgg aaagaacccg gcaaaagtgg
    11401 tctgttgcgc agcgaaatac atcagcagcg cgcctgcgaa catcaatacc gccagcactt
    11461 tttgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac aggatcgccg
    11521 caatggcggt acaggcatac gaccagccaa tttctccggc actaaaaccg cttttactta
    11581 accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca aacatcatga
    11641 acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa tgtaaggtac
    11701 gcgatgacaa taccacttaa caataccttt taaatacctt tgcggtgaaa acttgaccgc
    11761 gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat
    11821 ggaaaaactc gcccgtaacg accaggatta tgattgcccg caaaaggcgg gccaggacaa
    11881 tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    11941 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgacaatt ccttctgacc
    12001 agctttatct ccccggacat gactacgtag accgcgtaat gattgacaat aatcgcccgc
    12061 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    12121 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    12181 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    12241 actgtgtggc gtcaacttac ggcgtgttgg cgtcggtatc gcggcgctat gcgcatcgca
    12301 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    12361 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtacga
    12421 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    12481 aacgcgcgca cgagctgggc atggtgaccg tgctgtgggc ctatttgcga aactccgcct
    12541 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gaccggtcag gcaaaccatc
    12601 tggcggcaac catcggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    12661 ataaagcaat taattacggt tacaccgacg atcgtgttta tagcaaattg accagcgaaa
    12721 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    12781 taaactccgg cggtgctgcg ggcggtgaaa ctgacctcag tgatgcagtg cgtactgcgg
    12841 ttatcaacaa acgcgctggc ggaatggggc tgattcttgg acgtaaagcg ttcaagaaat
    12901 cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga cgtttatctc gatagcaaaa
    12961 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataaggcgtt
    13021 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    13081 agcactgcca cgtatgtcgg aaaaggcgtt tacaccgcat ccggcataaa aacacgcgca
    13141 ctttgctacg gcttccctat cgggaggccg tttttttgcc tttcactcct cgaacagttt
    13201 tcatattgtc gttttgtgat cgttatctcg acatttaaaa acaaatcatt tcattatatt
    13261 ttgaaatcga aaacaagcaa caggatatga aaatgtacgt ggtatcgaca aagcagatgc
    13321 tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa
    13381 cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa
    13441 cgcctggcac attcacttat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg
    13501 cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata
    13561 tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt
    13621 ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc tttgatgtca
    13681 gcgtcgaagc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag
    13741 ccgatgtttt ttataccaac cccgctcagg cgcgtgaatt tgccgaggca accggaattg
    13801 attccctggc ggtcgccatc ggcacggctc atgggatgta tgccagcgca ccggcgcttg
    13861 atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg
    13921 cgtcagggtt atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca
    13981 acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt aaaaaattac ctgaccgagc
    14041 accctgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg
    14101 tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt
    14161 taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc
    14221 atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc
    14281 aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt
    14341 tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtc
    14401 ggatttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa ctgctggcag
    14461 caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt
    14521 gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga tcccataccg
    14581 ttagcaccag aaacggttgc ggaacgagct gctttgcttt gctgcggaaa gtgtggcgac
    14641 agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg ttccgggcgg
    14701 tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg ccaatacttt
    14761 acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa cacgcgtgat
    14821 tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc aatattatcc attatcagcc
    14881 gcaggaagcg cagccgctgg cgcaatggat agaaaacacc cgaatggttt atgaagcaca
    14941 ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc actttgcaat
    15001 attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg cactggcaca
    15061 aattgagcag gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg taattgaaga
    15121 agtgatgctc gacgaaccgc aatactggaa aaaatattat cgtacgggtt ttaacgattc
    15181 attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc cgcatagtcg
    15241 gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa ggcgtggaca tcccactggg
    15301 catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg aattatcagc
    15361 aataccgcat cagctgatta tggataaaat ttatgatgtt ttgcgcgcct atcgctacgg
    15421 ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa tttcttttgt
    15481 cgatcgtagc gaagttttaa cccatatcgg taatgagatg ctcgccaaag gtgtggttca
    15541 tgatacctgg ccacaggcat taattgccag agaa
//
LOCUS       DAJTVE010000098        15409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000098 DAJTVE010000000
VERSION     DAJTVE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            11..454
                     /locus_tag="OO439_002932"
     CDS             11..454
                     /locus_tag="OO439_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6317245.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="OO439_002933"
                     /pseudo
     CDS             670..759
                     /locus_tag="OO439_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="OO439_002934"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="OO439_002934"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6317246.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTLLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="OO439_002935"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="OO439_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6317247.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="OO439_002936"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="OO439_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6317248.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="OO439_002937"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="OO439_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6317249.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="OO439_002938"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="OO439_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6317250.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="OO439_002939"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="OO439_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6317251.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="OO439_002940"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="OO439_002940"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6317252.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="OO439_002941"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="OO439_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6317253.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="OO439_002942"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="OO439_002942"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6317254.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="OO439_002943"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="OO439_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6317255.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="OO439_002944"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="OO439_002944"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6317256.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="OO439_002945"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="OO439_002945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6317257.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="OO439_002946"
     CDS             13056..13436
                     /locus_tag="OO439_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6317258.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="OO439_002947"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="OO439_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6317259.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="OO439_002948"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="OO439_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6317260.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15409)
                     /gene="nadA"
                     /locus_tag="OO439_002949"
     CDS             complement(15245..>15409)
                     /gene="nadA"
                     /locus_tag="OO439_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6317261.1"
                     /translation="SCAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNR
                     MLDFAATLRG"
BASE COUNT         3917 a         4044 c         3851 g         3597 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtagcgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaaa
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcagct
//
LOCUS       DAJTVE010000099        15227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000099 DAJTVE010000000
VERSION     DAJTVE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..751
                     /locus_tag="OO439_002950"
     CDS             <1..751
                     /locus_tag="OO439_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6317262.1"
                     /translation="ESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYE
                     LDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQE
                     VNAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLL
                     NFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTAS
                     LQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(792..1724)
                     /locus_tag="OO439_002951"
     CDS             complement(792..1724)
                     /locus_tag="OO439_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6317263.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2088..4874
                     /gene="polA"
                     /locus_tag="OO439_002952"
     CDS             2088..4874
                     /gene="polA"
                     /locus_tag="OO439_002952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6317264.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5256..5888)
                     /locus_tag="OO439_002953"
     CDS             complement(5256..5888)
                     /locus_tag="OO439_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6317265.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6470..6979
                     /gene="yihI"
                     /locus_tag="OO439_002954"
     CDS             6470..6979
                     /gene="yihI"
                     /locus_tag="OO439_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6317266.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7168..8541
                     /gene="hemN"
                     /locus_tag="OO439_002955"
     CDS             7168..8541
                     /gene="hemN"
                     /locus_tag="OO439_002955"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6317267.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8731..8841)
                     /locus_tag="OO439_002956"
     CDS             complement(8731..8841)
                     /locus_tag="OO439_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6317268.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8953..10362)
                     /gene="glnG"
                     /locus_tag="OO439_002957"
     CDS             complement(8953..10362)
                     /gene="glnG"
                     /locus_tag="OO439_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6317269.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10374..11423)
                     /gene="glnL"
                     /locus_tag="OO439_002958"
     CDS             complement(10374..11423)
                     /gene="glnL"
                     /locus_tag="OO439_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6317270.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11597..13006)
                     /gene="glnA"
                     /locus_tag="OO439_002959"
     CDS             complement(11597..13006)
                     /gene="glnA"
                     /locus_tag="OO439_002959"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6317271.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13379..15202
                     /gene="typA"
                     /locus_tag="OO439_002960"
     CDS             13379..15202
                     /gene="typA"
                     /locus_tag="OO439_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6317272.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3964 a         3800 c         3913 g         3550 t
ORIGIN      
        1 cgagtcgaaa tttaatcaga ttaacattat caatcacggg gaagacgttg cccaaatcga
       61 tgctttcgtt gcaaaaacca tgctggatcg cgttaaagac aaagattata tcaatgtcaa
      121 tctgacctac gaacttgata agttaacaaa agggaatcag caactcggta gtggtgagtg
      181 gtcattgatt gctgaatcta ttgatccctc agcagtgcgc caatttatca tccagtataa
      241 cattgctatg cagaagcagc ttgctgtaca ccctgagtta gcaaacgatg aagttgctct
      301 gcaagaagtg aatgctgcat tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc
      361 gaccattaaa caaccggtaa gatggaagaa cgcactcggc gaactaaatg ccaatctgga
      421 tatcagtatt gccgacccag ccaaatcttc atcatccaca aacaaagata tcaaattgct
      481 caattttgat gtgaagttac cgcttaatgt cgtcacagaa accgcaaaac agcttaattt
      541 atctgaagga atggatgcgg aaaaagcgca aaagcaggct gataaacaaa tcagcgggat
      601 gatgacattg ggtcagatgt ttcagttaat cacgattgac aacaataccg cctcgctgca
      661 gctgcgttat acaccgggta aagttgtgtt taacggacag gagatgagcg aagaagaatt
      721 tatgtctcgt gccggacgtt ttgttcatta aaatactcac tcgcctctgc tcattcagag
      781 gcgagtaaaa cttacttatt ctgacgctgt gatgacatga gagacgtcag aagccggtct
      841 ttttcctgcc acaatgagtt cagccattgc tgaaaatggc gcttaaagct tttgtcattg
      901 atgtagtcgc catgtaactc atcggcaata ggctgtaaat ccacatgtac gacaatccgc
      961 gtcagtttac cgcttaacat atcgaagaac ggctgacggt tattgtccgg ataacacagc
     1021 gtaacattaa gcagtttatc gaattgttta cccagtacat ttagcgccat cgcaatgcct
     1081 gccgcctttg gtggcaacaa gttttgaaaa gtggagtggg tttgctgatg tttttcttgc
     1141 gtgaaacggg agccttcaac gaaattgaca atggtggtgg gatgcaggcg aaacttttca
     1201 caagaacgcc gagtggtttc aacatcttta ccacgtcgct ccggatgacg taataaataa
     1261 gcgcgggaat aacgcttcat aaacggcata tccagcgccc agcacgccag gccaagaaat
     1321 ggcacccagg ccagctgttg cttgaggaaa tatttattca tcggaatgtg cttacgaaac
     1381 agcacgcaca gtacgacaat atctgcccaa ctacggtggt tacaaataag cagataccag
     1441 ttcttcttac ttaacccttc cagcccgtga acttcccatt gcaggtgtgg gttcagatgc
     1501 agtaataccg ccagaccttc acaccagcaa tacatcataa aatcacaaaa acgcgaaacc
     1561 tttcgccaaa tgactggcac aggtagcaat agttttacaa tcccggcaat gatgatcggg
     1621 acggaacaaa aaatagtgac caaaatggtc aatacgatac tcagcaaaag ggttatcgca
     1681 gcgagtattc tcgtcataat gaatttattc aaaaggttag ccatagacag tgcgctaaag
     1741 aagcgcaagg cgcagatttt agcagaaaac gacccaaata acggatgatc cttaaggaga
     1801 aaaataatcc atatctatcc acattagaaa aaatcccatt atctcaatta ttagggatgg
     1861 ctttattttt aactgcatga aaaacaaaga caaacatcat gctgtaaaaa gcatgataat
     1921 aaattaaaag cgatgtaaat aatttatgca caaagttatc cacatgacga tttgcgagcg
     1981 atccagaaga tctacaaaag attttcacga aaagcggtga aaaactcatg ttttcatcct
     2041 gtctgtggca tcctttaccc ataatctgat aaacaggcac ggacattatg gttcagatcc
     2101 cccaaaatcc acttatcctt gtagatggtt catcttatct ttatcgcgca tatcacgcgt
     2161 ttcccccgct gactaacagc gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca
     2221 tgctgcgcag tctgatcatg caatataaac cgacgcatgc agcggtggtc tttgacgcca
     2281 agggaaaaac ctttcgtgat gaactgtttg aacattacaa atcacatcgc ccgccaatgc
     2341 cggacgatct gcgagcacaa atcgaacccc tgcacgcgat ggttaaagcg atgggactgc
     2401 cgctgctggc ggtttctggc gtagaagcgg acgacgttat cggtactctg gcgcgcgaag
     2461 ccgaaaaagc cgggcgtccg gtgctgatca gcactggcga taaagatatg gcgcagctgg
     2521 tgacgccaaa tattacgctt atcaacacca tgacgaatac catcctcgga ccggaagagg
     2581 tggtgaataa gtacggcgtg ccgccagaac tgatcatcga tttcctggcg ctgatgggtg
     2641 actcctccga taacattcct ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc
     2701 tgcaaggtct tggcgggctg gatacgctgt atgccgagcc agaaaaaatt gctgggttga
     2761 gcttccgtgg cgcgaaaaca atggcagcga agctcgagca aaacaaagaa gttgcttatc
     2821 tctcatacca gctggcgacg attaaaaccg acgttgaact ggagctgacc tgtgaacaac
     2881 tggaagtgca gcaaccggca gcggaagagt tgttggggct gttcaaaaag tatgagttca
     2941 aacgctggac tgctgatgtc gaagcgggca aatggttaca ggccaaaggg gcaaaaccag
     3001 ccgcgaagcc gcaggaaacc agtgttgcag acgaagcgcc agaagtgacg gcaacggtga
     3061 tttcttatga caactacgtc accatccttg atgaagaaac actgaaagcg tggattgcga
     3121 agctggaaaa agcgccggta tttgcatttg ataccgaaac cgacagcctt gataacatct
     3181 ctgctaacct ggtcgggctt tcttttgcta tcgagccagg cgtagcggca tatattccgg
     3241 ttgctcatga ttatcttgat gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc
     3301 taaaaccgct gctggaagat gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc
     3361 gcggtattct ggcgaattac ggcattgagc tgcgtgggat tgcgtttgat accatgctgg
     3421 agtcctacat tctcaatagc gttgccgggc gtcacgatat ggacagcctc gcggaacgtt
     3481 ggttgaagca caaaactatc acttttgaag agattgcggg taaaggcaaa aatcaactga
     3541 cctttaacca gattgccctc gaagaggccg gacgttacgc cgccgaagat gcggatgtca
     3601 ccttgcagtt gcatctgaaa atgtggccag atctgcaaaa acacaaaggg ccgttgaacg
     3661 tcttcgagaa tatcgaaatg ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg
     3721 tgaagatcga tccgaaagtg ctgcacaatc attctgaaga gctcaccctt cgtctggctg
     3781 agctggaaaa gaaagcgcat gaaattgcag gtgaggaatt taacctttct tccaccaagc
     3841 agttacaaac cattctgttt gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg
     3901 gcgcgccgtc aacgtcggaa gaggtactgg aagaactggc gctggactat ccgttgccaa
     3961 aagtgattct ggagtatcgt ggtctggcga agctgaaatc gacctacacc gacaagctgc
     4021 cgctgatgat caacccgaaa accgggcgtg tgcatacctc ttatcaccag gcagtaactg
     4081 caacgggacg tttatcgtca accgatccta acctgcaaaa cattccggtg cgtaacgaag
     4141 aaggtcgtcg tatccgccag gcgtttattg cgccagagga ttatgtgatt gtctcagcgg
     4201 actactcgca gattgaactg cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga
     4261 ccgcattcgc ggaaggaaaa gatatccacc gggcaacggc ggcagaagtg tttggtttgc
     4321 cactggaaac cgtcaccagc gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga
     4381 tttatggcat gagtgctttc ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc
     4441 agaagtacat ggacctttac ttcgaacgct accctggcgt gctggagtat atggaacgca
     4501 cccgtgctca ggcgaaagag cagggctacg ttgaaacgct ggacggacgc cgtctgtatc
     4561 tgccggatat caaatccagc aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta
     4621 acgcgccaat gcagggaacc gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg
     4681 cgtggttaca ggctgagcaa ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg
     4741 tatttgaagt tcataaagat gatgttgatg ccgtcgcgaa gcagattcat caactgatgg
     4801 aaaactgtac ccgtctggat gtgccgttgc tggtggaagt ggggagtggc gaaaactggg
     4861 atcaggcgca ctaagattcg cctgaacatg cccttttttc gtaagtaagc aacataagct
     4921 gtcacgtttt gtgatggcta ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa
     4981 agtgctttct gaactgaaca aaaaagagta aagttagtcg cgtagggtac agaggtaaga
     5041 tgttctatct ttcagacctt ttacttcacg taatcggatt tggctgaata ttttagccgc
     5101 cccagtcagt aatgactggg gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg
     5161 atgcgctatg tttatcaggc caacggtaga attgtaatct attgaattta cgggccggat
     5221 acgccacatc cggcacaagc attaaggcaa gaaaattatt cgccgtcctg cgtttcttct
     5281 acaggctgca tctcgctaaa ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt
     5341 ttcttcaacg aagaaaacgt ttcaacctgc acatcaccgt taaacgccag tacagcttca
     5401 cgcaccatat tcaattgcgc tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc
     5461 agcaccagaa cggcgatatt gctgtctacc gcccactcaa tcatctgctg atccaaatct
     5521 ttcagcggat ggcgaatatc cattagcacc accagacctt gcaggctctg acgtttttcg
     5581 aggtattcgc cgagcgcacg ctgccatttg cgcttcatct cttccgggac ttccgcataa
     5641 ccgtacccag gcaagtcaac cagacgcttg ccgtcagcca cttcaaacag gttgataagc
     5701 tgggtgcgcc ctggggtttt tgaggtacga gccaggcttt tctggttagt cagcgtgttc
     5761 agcgcgctgg atttacctgc gttggaacgg cctgcaaaag ccacttcaat tccggtatcg
     5821 gaaggtaggt ggcgaatatc aggcgcactc atcacaaaat gcgtctgttg ataattcaaa
     5881 ttagtcaaag cggtcgtctc cgtcagtcaa agctttgtgg cgattatacc tgaaccataa
     5941 taaaaggcgg atttttcggc gtgagcgttg taagtaaaag ccatacgctt tgtgagacat
     6001 tgccgatagt ctttatgcga aatagcagag aaaattctgc gatgcatgtc aaataagcta
     6061 tataaatcag tgaattgctt ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt
     6121 tgattgtttg tttaaagcaa aggcgtaaag tagcacccat agagcgagga cgctaacagg
     6181 aacaatgact caggatgagg gtcaggagcg ccaggaggcg aagacagagg attgtcagga
     6241 agacaaacgt ccggagacgt aattaaacgg aaatggaatc aacacggatt gttccggcta
     6301 aaggaaaaac agggtgtgtt ggcggcctgc aaggattgta agacccgtta agggttatga
     6361 gtcaggaaaa aaggcgacag agtaatctgt cgcctttttt ctttgcttgc tttctgttag
     6421 attccgccgc aaatctatac tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc
     6481 atcttcaaac tcacgcagca aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct
     6541 ggatcaggaa gctcgtgatc gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag
     6601 ccgcgcagcg ggcggaaaca ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc
     6661 acgtattggc agtaaaaccc ctattccatt gggcgtggct gaaaaagtca ccaaacagca
     6721 caaaccgaag agtgagaaac ctatgctttc accgcaggcg gagttggaat tactggaaac
     6781 ggatgagcgt ctggatgcgc tgctggaacg tctggaagca ggcgaaaccc tgagcgccga
     6841 agagcaatcc tgggtggatg ctaaactgga tcgtattgat gagttgatgc agaaactcgg
     6901 cctctcttat gacgatgacg aagaagagga agaagacgag aagcaagaag acatgatgcg
     6961 tctgctgcgg ggtaactaac ggattgccac cgtgggcctt cccgttctgc tgataaccct
     7021 tccggttata tgttatctgg tgtggttatt cgttaaacta cagcggttgt cgcggcgaca
     7081 aaagtggctg cgcaaccggc tgatgacccg aaacgggcat ccgccggtac gccgtagccg
     7141 ccagagacgc catcggaagg agtgagcatg tctgtacagc aaatcgactg ggatctggcc
     7201 ctgatccaga aatataacta ttccgggcca cgatacacct cgtacccgac cgcgctggag
     7261 ttttcagaag acttcggcga acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt
     7321 ccattatctc tctacgtaca tatcccgttc tgccataagc tttgttactt ctgcggttgc
     7381 aataagattg ttactcgcca gcagcacaag gccgatcagt atctggacgc gctggagcaa
     7441 gaaatcgtcc atcgtgcacc gctgttcgcc gggcgtcacg tcagccaatt gcactggggc
     7501 ggcggaacgc cgacgtatct gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc
     7561 gaaaacttcc agttcaatgc cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc
     7621 gaactggatg tactcgatca tttacgcgcc gaaggcttta atcgcctgag catgggcgtg
     7681 caggacttca acaaagaagt gcaacgtctg gttaaccgcg agcaggatga agagttcatc
     7741 tttgcactgc ttaaccatgc gcgtgagatt ggttttacct ccaccaacat cgacctgatt
     7801 tacggcctgc cgaaacagac gccggagagt ttcgccttta ccctgaaacg tgtggcggag
     7861 ctgaaccccg atcgtctgag tgtctttaac tacgcgcatc tgccgaccat ttttgctgct
     7921 cagcgcaaaa tcaaagatgc tgacctgccg agtccgcagc aaaaactcga tatcctgcag
     7981 gaaaccatcg ccttcctgac gcaatcgggc tatcagttta tcggtatgga tcactttgcc
     8041 cgtccggatg acgagctggc ggtggcccag cgtgaaggcg tgctgcatcg taacttccag
     8101 ggctacacca ctcagggcga taccgatctg ctggggatgg gcgtttccgc catcagcatg
     8161 attggcgact gctacgcgca gaaccagaaa gagttgaagc agtactatca gcaagtggat
     8221 gaacaaggca atgctctgtg gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc
     8281 gatgtgatta agtcgctcat ctgcaacttc cgtctggatt acgcccctat tgagaaacag
     8341 tgggatttgc acttcgctga ttactttgcg gaagatctca agctgctcgc cccgttagca
     8401 aaagatgggc tggtggatgt ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg
     8461 ctgatccgca acatttgcat gtgctttgat acctatctgc gccagaaagc gcggatgcag
     8521 cagttctcac gggtgattta aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa
     8581 gcgccatcaa tgtgctgaga acaagattgc ctgatgcgct acgcttatca ggcctacaaa
     8641 acctgttgaa aatattgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
     8701 acaaagagca cttcatcagc aatctgaaac tcaactaaac agcccaatca ttgcggcaca
     8761 cagcacggca gcaacggcag tttgcggtgt gtggctgtca actgcgccac tcgataccag
     8821 attaattatt gattccagca taatgactct ccccgttttc cgggcaagat catactgaac
     8881 ttatcggaac agtaaagcgt aaaataccag caatttgcgc tcaataatca atctttacac
     8941 acaagctgtg aatcactcca tccccagctc ttttaactta cgcgtcaggg tgttgcggcc
     9001 ccagccgagt agccgcgccg cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt
     9061 cagtaacgtc cgctccagct ctggctgcgc ttcggaaagc agattttgat gaccggaacg
     9121 cagcgctctg tctgcccact gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga
     9181 agtactctcc gcaaccgttg attcaaacag ttcgccgggc aaatcctgaa tcaacacttc
     9241 ctgcccggcg gccatcaccg ttagccagcg gcaggtgttt tccagctggc gcacgttgcc
     9301 tggccacgcc agacgcgtca gagcagcttc ggtttccgga tgcagtaact tcgcttctac
     9361 gcccagttcg cgcgcggcaa cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg
     9421 acgttcgcgc agcggcggca gatgaacgcg gataacgttc aggcggtgga acagatcctc
     9481 acggaactta ccttcctgca ctcgctgttc gagattctgg tgagtggcag cgataatacg
     9541 cacatccact ttcaccggcg catagccgcc aacgcggtaa aactgaccgt ctgccagcac
     9601 gcgcagcaaa cgcgtctgca catccagcgg catatcacca atttcgtcga ggaataatgt
     9661 accgccatcg gcctgttcaa aacgcccctg acgaatggta ttcgcgccag taaacgcgcc
     9721 tttctcgtgg ccaaacagtt ctgattcgat caaatctttt gggatagctg ccatattcag
     9781 cgcgataaac ggcgctttgg cgcgcggact gtggcgatgc agggcatgag cgaccagttc
     9841 tttaccggtg ccggattcgc cgttaatcag cacgctaata gaagaacgcg aaagccgacc
     9901 gataatacgg aacacgtcct gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc
     9961 gttaagctga atattacgcg gctgctgctg ttcctggtaa tgactgatag cgcgctcaac
    10021 cagcgccact gcttcgtcga tatcaaacgg tttgggcaga taatcaaacg ccccttgttg
    10081 ataggcgctg acggcagcat ccagatcgga atgtgcggtc ataatgatga ccggaagcat
    10141 tggatggcgc tgtttaatct gcttgagcag cgccagcccg tccattcccg gcatacggat
    10201 atctgaaagc agcacatccg gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt
    10261 ctcaaatgtc gtacaggtta aacccgctcc agcgagcgca cgttcaagca cccaacggat
    10321 ggaactatcg tcatcgacta cccagactat ccctcgttgc ataaacgtca cctttatttc
    10381 ctgataggca ggtaaaccga gaactcggta tgacctggcc aactggtaaa ttcaattttg
    10441 cctgaatgct gatcaatcaa attacgggcg atggataagc caagcccggt gccaccttcg
    10501 cggccgctga ccatcgggta aaacagcgta tcctgcaaat gaggcggaat gcccggcccg
    10561 ttatcttcca catcaatccg cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga
    10621 aacgcggtgc gggtacgcag aatgatttca ccgccttccg gccccagcgc ctgtagcgca
    10681 ttgcgcacaa tattcagcaa gacctgttca atttgatccg ggtcgtgcgc cagttccggc
    10741 aggctggggt cgtaatcacg aatcaaccgc acgttgtccg gcagttccat cgacaccagc
    10801 gtcaccacgc gttcagctac tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc
    10861 ggccccaaca gacggtcgac cagatttcgc agccggtccg cctgttcgat aatcactttg
    10921 gtatattcga gtagtgatgg gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt
    10981 aaacctccaa gcggattttt aatctcatgt gccaggccgc gcactaaatc acgggcagca
    11041 acctgctggg cgtgctgtag ctgttcctga cttaagcggc gctggttatc catcggagcc
    11101 atctccagca ggatcatgcc gtccggcata cgctgggccg tcacagaaag gatatgcgag
    11161 cgcccgtcga tgaccagcgt cacttcgtta tcggtaaaac cttgccccgc ctccagactt
    11221 tcttgcatca gctcgatatt taatgagaag tagctcaaca gttccggtaa cggtgtacca
    11281 aacaatttgc gggagctttg ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc
    11341 gccagattgt catcaattaa caaaatactg ttaatcagcg agttgaggat ctgcccagca
    11401 tcgggctgcg tgcctgttgc cataaagcag tctcctgaac aggttgcacc attttagtgc
    11461 attatagctt tttacggata aaaagcgcga agcatcagag aattgacgga gaaaaaagcc
    11521 catgcagaga tgggctgaaa gtttccacgg caactaaatc ccggcgttgt tgcgccgggt
    11581 agtacaactt caactattag acgctgtagt acagctcaaa ctctaccgga tgcggagtca
    11641 tacgcacgcg gtcatcttct tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag
    11701 tgaacacgcc accggctttc aggaactcgc ggtccagatc cagttcgttc agtgcttctt
    11761 ccagagagcc tgcaacctgt gggatctctt tcgcttcttc tggcggcagg tcatacaggt
    11821 ttttgtccat ggcttcgccc ggatggatct tgttcttgat accatcaaga ccggccatca
    11881 gcagggcagc aaagcacagg tacgggttag ctgccggatc cgggaaacgt acttcgatac
    11941 gacgtgcttt cggagaagaa accaccggaa tacggataga cgcagaacgg ttacgcgcag
    12001 agtaagccag cattaccggt gcttcatagc ccgggaccag acgcttataa gagttggtgg
    12061 tcgggtttgc cagggcgtta atcgctttag cgtgtttgat tacgccgcca atgtagtaca
    12121 gcgcctgctc agacagacct gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag
    12181 acagagacat gtggcagtgc ataccggagc cgttatcacc gaacatcggt tttggcataa
    12241 aggtcgcggt tttaccgaag cggtgcgcta cgttgtgcac aacatatttg tagatctgaa
    12301 tttcgtcagc ttttttggtc atagtattga agcgggtagc cacttcgttc tgaccagcag
    12361 tcgctacttc gtggtgatgg gcttcaacca ccagacccat ctgttccatc accagacaca
    12421 tttcagaacg aatatcctga gccgagtcta ccggtggaac cgggaagtaa ccgcctttca
    12481 ctgccggacg gtgacctttg ttaccacctt cgtattgggt ggaggagttc catgcgcctt
    12541 cgatatcgtc gatagcaacg tgggaaccgg agatagatga tccgaaacgg atgtcatcga
    12601 acaggaagaa ttcaggttct ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca
    12661 ggtaatcttc ggcgcgcttc gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc
    12721 caggttcaag gatgtcgcaa cggataatca gggtggagtc ggcgaagaac gggtcaatca
    12781 ctgcggtgga tgcgtctggc atcagcacca tgtcggactc gttaatgcct ttccagccgc
    12841 caatcgagga gccgtcaaac attttgcctt cttcgaagaa ttcagcattc acctgatgag
    12901 cagggatagt gacgtgctgt tctttacctt tagtatcggt gaagcgcaaa tcaacaaact
    12961 tcacttcgtg ctcgttcagc atcgtcagta cgtgttcagc ggacatactt taactctcct
    13021 ggattggtca tggtcgtcgt ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa
    13081 aaaagataaa gcgaaatctg tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg
    13141 caccatcgtg caaaagggct gcaccacgat gtgaatgttg caccaatata gtgcttcaat
    13201 ggaaacatta agcaccatgt tggtgcaatg acctttggat aacccttttt atgctccgtg
    13261 aaagcgatca caaagggact ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt
    13321 tgtagtgcga ttaatccgtg tacaataacg cgctatttct aatgcctgag gcaaagttgt
    13381 gatcgaaaaa ttgcgtaata tcgccatcat cgcgcacgta gaccatggta aaaccaccct
    13441 ggtagacaag ctgctccaac aatccggtac gttcgactct cgtgccgaaa cccaagagcg
    13501 cgtgatggac tccaacgatt tggagaaaga gcgtgggatt accatcctcg cgaaaaacac
    13561 cgctatcaaa tggaatgatt accgtatcaa catcgttgat accccggggc acgccgactt
    13621 cggtggtgaa gttgaacgtg taatgtccat ggtagactca gtgctgctgg tggttgacgc
    13681 atttgacggc ccgatgccgc aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct
    13741 gaagccgatt gttgttatca acaaagttga ccgccctggc gcgcgtcctg attgggttgt
    13801 ggatcaggta ttcgatctgt tcgttaacct cgacgcgacc gacgagcagc tggacttccc
    13861 gatcgtttac gcttctgcgc tgaacggtat cgcgggtctg gaccacgaag atatggcgga
    13921 agacatgacc ccgctgtacc aggcgattgt tgaccacgtt cctgcgccgg acgttgacct
    13981 tgacggtccg ttccagatgc agatttctca gctcgattac aacagctatg ttggcgttat
    14041 cggcattggc cgcatcaagc gcggtaaagt gaagccgaac cagcaggtca ctatcatcga
    14101 tagcgaaggc aaaacccgca acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga
    14161 acgtatcgaa accgatctgg cggaagctgg cgatatcgtg gcgatcacgg gccttggcga
    14221 actgaacatt tctgacaccg tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc
    14281 cgttgatgag ccgaccgttt ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa
    14341 agaaggtaag ttcgtaacgt ctcgtcagat cctggatcgt ctgaacaaag aactggtaca
    14401 caacgttgcg ctgcgcgtag aagaaaccga agacgccgat gcgttccgcg tttctggtcg
    14461 tggcgaactg cacctgtctg ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc
    14521 ggtatcccgt ccaaaagtaa tcttccgtga aatcgacggt cgtaaacaag agccgtatga
    14581 aaacgtgact ctggacgttg aagaacagca tcagggttct gtaatgcagg cgctgggcga
    14641 acgtaaaggc gacctgaaaa acatgaatcc agacggtaaa ggccgcgtac gtctcgacta
    14701 cgtgatccca agccgtggtc tgattggctt ccgttctgag ttcatgacca tgacttccgg
    14761 tactggtctg ctgtactcca ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg
    14821 tcagcgtcag aacggcgtac tgatctctaa cggtcagggt aaagcggtcg cgttcgcact
    14881 gttcggtctg caggaccgcg gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg
    14941 tcagattatc ggtattcaca gccgctctaa cgacctgact gtaaactgcc tgaccggtaa
    15001 gaaactgacc aacatgcgtg cttccggtac tgacgaagct gttgttctgg ttccgcctat
    15061 ccgcatgact ctggaacaag ctctggagtt catcgatgat gacgaactgg tagaagtgac
    15121 tccgacctct atccgtattc gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa
    15181 ccgcgcaccg aaagacgatt aatttcgttt tttagttata aaaaacc
//
LOCUS       DAJTVE010000100        15218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000100 DAJTVE010000000
VERSION     DAJTVE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..236)
                     /locus_tag="OO439_002961"
     CDS             complement(<1..236)
                     /locus_tag="OO439_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HCR6317273.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRP"
     gene            complement(566..1534)
                     /gene="astE"
                     /locus_tag="OO439_002962"
     CDS             complement(566..1534)
                     /gene="astE"
                     /locus_tag="OO439_002962"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6317274.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1527..2870)
                     /gene="astB"
                     /locus_tag="OO439_002963"
     CDS             complement(1527..2870)
                     /gene="astB"
                     /locus_tag="OO439_002963"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6317275.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2867..4345)
                     /gene="astD"
                     /locus_tag="OO439_002964"
                     /pseudo
     CDS             complement(2867..4345)
                     /gene="astD"
                     /locus_tag="OO439_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4342..5376)
                     /gene="astA"
                     /locus_tag="OO439_002965"
     CDS             complement(4342..5376)
                     /gene="astA"
                     /locus_tag="OO439_002965"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6317276.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5373..6593)
                     /gene="astC"
                     /locus_tag="OO439_002966"
     CDS             complement(5373..6593)
                     /gene="astC"
                     /locus_tag="OO439_002966"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6317277.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7039..7845
                     /gene="xthA"
                     /locus_tag="OO439_002967"
     CDS             7039..7845
                     /gene="xthA"
                     /locus_tag="OO439_002967"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6317278.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8012..8722
                     /locus_tag="OO439_002968"
     CDS             8012..8722
                     /locus_tag="OO439_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6317279.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8727..9404
                     /gene="ydjY"
                     /locus_tag="OO439_002969"
     CDS             8727..9404
                     /gene="ydjY"
                     /locus_tag="OO439_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6317280.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9419..10126
                     /locus_tag="OO439_002970"
     CDS             9419..10126
                     /locus_tag="OO439_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6317281.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10126..10674
                     /locus_tag="OO439_002971"
     CDS             10126..10674
                     /locus_tag="OO439_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6317282.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10684..11850
                     /locus_tag="OO439_002972"
     CDS             10684..11850
                     /locus_tag="OO439_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6317283.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            11823..13359
                     /locus_tag="OO439_002973"
                     /pseudo
     CDS             11823..13359
                     /locus_tag="OO439_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13359..14012
                     /locus_tag="OO439_002974"
     CDS             13359..14012
                     /locus_tag="OO439_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6317284.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14079..>15218
                     /gene="ynjE"
                     /locus_tag="OO439_002975"
     CDS             14079..>15218
                     /gene="ynjE"
                     /locus_tag="OO439_002975"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6317285.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQ"
BASE COUNT         3515 a         4145 c         3980 g         3578 t
ORIGIN      
        1 ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg tttctgcgcg
       61 tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg
      121 tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg
      181 ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc
      241 tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag
      301 attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta
      361 aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagag
      421 aatattacaa cacaatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
      481 aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa cttaataccc
      541 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
      601 atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa
      661 aacgttcctc tccgtcctgc gccagcaatg ttcctttctc aaacggcata aaattcagcg
      721 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
      781 gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
      841 ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc
      901 caagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac
      961 caccaggctc ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagaa
     1021 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgagga tgcaaggagc
     1081 cacgaattgc ggtatgtaga tcaaggtgcc aacgcacaga ttctttgccc tggtcataaa
     1141 aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt tcagcaaata
     1201 gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct
     1261 tcagcgcagg aggatccccg aggatcacca gcaaccgcca acgtaacggg atctcgccgt
     1321 gagatatcgc gccaagcaac gcgtccagca tttccacagg tgccgtctca ttaccgtgga
     1381 tccccgcaga aatcaccagc gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
     1441 catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata accggttttt
     1501 tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
     1561 taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc
     1621 agcaattgcg ggtcggccag atcggcagca gtaaggcgat cgcggtagta acgatccacc
     1681 cagtcattga gcgcattaaa cagcgtatcg ttcatcatca ccgccggatt caccgcccgg
     1741 cgttcttctt ctgtcaatac tacccgcaac cgcaggcacg ccgggccgcc gccattcgcc
     1801 atgctttcac gtaaatcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt
     1861 tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc
     1921 atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga caccgcatcc
     1981 gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcccgc
     2041 aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     2101 cggttactta cggcaatcac gtcattatga aaaacaccct ggtcgataac gtccgggttt
     2161 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     2221 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     2281 ccgtagacaa aaagttgcat acccggctca ccgtaatgac cgccgagacg attgtggttt
     2341 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     2401 tcttcgtcgt taaaaatagc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     2461 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt taccatccag cgtatcggca
     2521 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggagct gacgctggaa
     2581 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     2641 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac ggcctggggg
     2701 aatcccgcat cggcaagggt tttcattttc agtaagccct gtttcgccgc cagtcgcggg
     2761 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gccagcgtaa
     2821 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     2881 catcggaaaa atccagcccg gggttaagcg ttgcgggcaa agttaacgag tccgactcca
     2941 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     3001 cggatgcacc aatgccgccg aatggcgcgg tactggcagc accagtaagc ggtttgttcc
     3061 agttaacaat ccccacccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     3121 aaaccagacc gcaagagagt ccgaagcgag tgttattcgc cattagaatc gcttcctcga
     3181 aagaatcata acgccagacg cgcaataacg gtccgaacac ctcttcatct ggtacgccag
     3241 caacgcctgt catttcaatg atccccggcg tcagcaacga tgtctctgat tgtaataagc
     3301 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgctaggca gtaaccacct
     3361 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
     3421 tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca tcgccctgtg
     3481 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
     3541 ccgactgaat ggtcagatgg acagccgagt cgatatccgc cacctcatcg ataattagcg
     3601 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
     3661 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
     3721 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
     3781 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
     3841 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
     3901 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
     3961 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
     4021 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
     4081 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
     4141 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
     4201 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
     4261 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
     4321 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
     4381 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
     4441 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
     4501 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
     4561 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
     4621 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
     4681 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
     4741 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
     4801 atcgacgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
     4861 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
     4921 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
     4981 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
     5041 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
     5101 gccgacccga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tggcgcaaat
     5161 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
     5221 gggcagttcg ccttgccagg ttttgattgc cctttcgata cgcgcggaaa gcgtggcttc
     5281 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
     5341 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
     5401 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
     5461 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccataacgcc tgctttcgcc
     5521 gcctcctgag agatctgttt cgcttgtccg gcgtaatcgg cattcagtac acagccaatc
     5581 aacaaaccta agccgcgaac ttcactgaac aaaccgtagc ggtgattaat ggtattaaga
     5641 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
     5701 agctccagca ctttgcccgc caccgccgag gccagcggat tgccgccata ggtggtgcca
     5761 tgagtgccaa cggtcatcac gctggcgcac tcttcggttg ttaacaacgc accgaccggg
     5821 aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gtgtcacgcc gtagtgcata
     5881 taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa aatcaacagc
     5941 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
     6001 acaccgcctt ccccctggat gggttcgaca atcaccgcac aggtggcgtc gtcaatcagc
     6061 gcgctggcag agttaatatc gttatatgca gcatgacgga tatctggtgg cagtggcgca
     6121 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
     6181 aacgcatttt taaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat
     6241 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
     6301 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
     6361 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
     6421 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
     6481 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
     6541 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
     6601 tctacagtaa ataattcgct atctatatgt taacaataag taatgtttgc gctgtaaatg
     6661 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg
     6721 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
     6781 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
     6841 ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
     6901 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
     6961 cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct
     7021 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
     7081 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
     7141 acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg
     7201 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
     7261 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg
     7321 gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
     7381 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac
     7441 tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc
     7501 agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc
     7561 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
     7621 ttggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat
     7681 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
     7741 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
     7801 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
     7861 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
     7921 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
     7981 tattatcaag tttgatatag gaaactccac gatgaatgct gagcgtaaat ttcttttcgc
     8041 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
     8101 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
     8161 ctatattctg ttattcatca ttgccaccct ctttgtgtta ccaggaagca tactggtgat
     8221 cgctggagga atagtttttg gcccgctctt agggacacta ctctcattaa ttgccgccac
     8281 gctggcctcc tcgtgctcat tcctgctggc gcggtggctg ggacgtgatt tactgctgaa
     8341 atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat
     8401 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
     8461 ttacggatta accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct
     8521 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
     8581 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctcg tccagctcgc
     8641 aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccgcttac
     8701 tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag
     8761 gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
     8821 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
     8881 ccaataacca tacggtaacc atgttggtgc aaattaatgg tcgtttcctc accgacgaca
     8941 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt
     9001 atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
     9061 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
     9121 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
     9181 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
     9241 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
     9301 atggtgcggt ggaaaaacgc ggcgaagtga aattcaaagg caatgcctca gttctcccgg
     9361 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccag gataaaggat
     9421 gatgaagatg caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
     9481 cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
     9541 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
     9601 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
     9661 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
     9721 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
     9781 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt
     9841 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
     9901 tccaatcagc tatgctgccg atctgacttc aatacgtttt cgctcgtttt ttatcgccac
     9961 cgggcttggt caattaccga cgactattgt ttattcctgg gcgggcagca tgttaacagg
    10021 cggtactttc tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt
    10081 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg
    10141 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    10201 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca
    10261 atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt
    10321 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gtgtggatat caccagtatg
    10381 aaagtcgccg agcgtaccgg cagcagcgat aaactgctgg cagtggctga ctggcggcaa
    10441 aacccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    10501 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    10561 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    10621 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggagag
    10681 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    10741 gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc
    10801 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    10861 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    10921 attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct gctctgggtg
    10981 aacggcgaaa acttccgcac cttaaaagag gccaatttat tacaaacggg ctgggcggag
    11041 actctgccca actggcgcta tgtcgacaca cagctgccgg tgcgggaaga tttttcagtg
    11101 ccgacacaag gtgcggaatc gccctggggc ggcgcacaac tgacgtttat cgcccgccgc
    11161 gatgttacgc cacagccacc acaaacgccg caagccttac tggagtttgc taaagccaat
    11221 cccggcacgg ttacttatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag
    11281 ttgctgatta tgctgacgcc agatcccgcc gcattaaaag aagcgccgga cgatgcgact
    11341 ttcgcccgtg tcactgcgcc cttgtggcaa tatcttgatg cactgcatcc gtatttgtgg
    11401 cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg atgctctgct gaaagccggc
    11461 acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc
    11521 gatttgccgg caagcagtta cagttttggc tttcgcgagg ggatgattgg caacgtgcat
    11581 ttcgtcacca ttccagccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc
    11641 ctgctctcac ccgatgcgca actgcgtaaa gcagatcccg ttgtctgggg cgatccttct
    11701 gttctcgatc cgcaaaaact gcctgacggg cagcgcgaaa tattgcaatc aagaatgccg
    11761 caggatctgc cgccggtact ggctgaaccg cacgcaggat gggtgaatgc actggaacaa
    11821 gaatggttac gccgttacgg tacgcattaa tctttttgct gtgggcgatg gtggcggtga
    11881 tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgcgttg tcgttgacac
    11941 actggcaggc gttatttgcc gatccacagt taccgcatgc attactggca acgctggtat
    12001 cgacaaccat cgcggcggtc ggggcattgt tgattgccct gctggtgatt gtggcgctat
    12061 agcctgggcc gaaatggcag cgtatgtgcg cccgtctgcc gtggctgctc gccattcccc
    12121 atgtggcttt tgccaccagc gccctgctgc tctttgctga cggagggctg ctatatgact
    12181 atttcccgta tttcactccg ccaatggacc gatttggcat cggtctgggc ctcacccttg
    12241 cagtgaaaga aagcgcattt ctgctgtgga tcttagcggc agtattgagc gaaaaacggc
    12301 tgttgcagca ggtcattgtg ctggattcgc tgggctacag ccgctggcaa tgcctgaact
    12361 ggctgctgtt gccctccgtc gcgccttcgc tggcaatggc gatgctggcg attgttgcct
    12421 ggtcgctttc ggtcgtggat gtggcgatta ttcttgggcc aggtaatcca ccgacgctgg
    12481 cagtaattag ctggcagtgg ttaactcagg gcgacgcgga tcaacaaacg aaaggcgcac
    12541 tcgccagtct gctgctgatg ctgttactcg ccgcctacgt tttgctgagc tatctgctat
    12601 ggcgcagctg gcggcgcact attccccgcg tagatggcat tcgcaatgcc tgccacacct
    12661 ttattaccag gcactacgct ggcgagtttt ttacccttaa ccggtgtgct gtgtgtggtt
    12721 ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg aagcactcat caacagtctg
    12781 acaatggggc tggtagcaac attcatcgct ttgctcctgt tactgctgtg gctggaatgg
    12841 gggccacagc gtcgccagtt gtggctatgg ttgcccattc tattgcccga tctgccactg
    12901 gtggcgggcc agtacacgct ggcgctatgg ctgaacctgg atggaagctg gacggcggtg
    12961 gtctgggggc atctgctgtg ggtaatgccg tggatgctgt ttatcctgca acccgcctgg
    13021 cagcgcattg attcacggtt aattttgatt gcgcaaacac tgggctggtc gcgggccaaa
    13081 atcttctttt acgtgaaatg cccactcatg ttgcgccctg tgctgattgc cttcgcggtg
    13141 ggattttcag tcagtattgc gcagtatatg ccgacgctgt ggctgggcgc ggggcgtttt
    13201 ccgacgctca ccactgaggc ggtggcatta agcagcggcg gcagcaacgg tattctcgcc
    13261 gcccaggctt tatggcaact gttgttaccg cttattattt ttgccctgac cgcgttagtc
    13321 gcaaaatggg taggttatgt cagacaagga ctccgctaat gctctgcgtg aaaaatgttt
    13381 cgctacgttt accagaaagc cgcttgctga caaacgttaa ctttacggtt gataaaggtg
    13441 acatcgtcac gttaatgggg ccgtctggct gtggaaaatc cactctgttt tcatggatga
    13501 ttggtgcact ggctggacag ttttcttgta caggtgagct atggctcaat gagcaacgga
    13561 ttgacatgct acccaccgca cagcgtcaga ttggcattct tttccaggat gcactgttat
    13621 ttgaccagtt cagcgtcggg caaaatttac tgctggcgct actgtcgacg ctcaagggga
    13681 ctgccaggcg caatgccgtg aaagacgcgc ttgatcgagc gggactggct gaaacttatc
    13741 accaggaccc tgcgacatta tcaggcggtc agcgagcgcg ggtggccctg ctgcgcgccc
    13801 ttctcgctca gccaaaagcg ttactcctgg atgagccatt cagccgtctt gatgtggctc
    13861 tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg cgaactggcg atccccgtcg
    13921 ttcaggtaac gcacgatctc caggatgttc ctgctgatag ttctgttctg gatatggcgc
    13981 agtggtcaga aaattacaac aaactgcgat aacgcaaagt ttttctcaat gcgtcagttc
    14041 agaatggcgc actcaaaact acaatgtcgg gattttcgat gaaacgtgtt tctcaaatga
    14101 ccgcgctggc aatggcttta gggctggctt gcgcttcttc gtgggccgct gaactggcga
    14161 agcctcttac acttgaccag cttcaacaac aaaatggcaa agcgatagat actcgcccca
    14221 gcgcgtttta taacggctgg ccacaaacct taaatggccc ttctggtcat gaacctgccg
    14281 ccttaaacct ctctgccagc tggctcgaca aaatgagcac cgaacagctc aacgcgtgga
    14341 tcaagcaaca taacctgaaa gccgatgccc cggtggcgct gtacggtaat gacaaagatg
    14401 tcgacgccgt caaaacgcga ctgcaaaaag caggttttac gcatatctcc attctgagtg
    14461 acgcgctaag cgaaccttcc cgtctacaaa aactgccgca ctttgagcag ctggtttatc
    14521 cgcaatggct gcacgacctg caacaaggta aagaggttac ggcgaaacct gccggtgact
    14581 ggaaagtcat tgaagcggcc tggggcgctc ctaagcttta ccttatcagc catattcccg
    14641 gtgctgacta catcgatacc aacgaagtgg aaagcgaacc gctgtggaac aaagtttctg
    14701 atgaacaact aaaagcgatg ctggcaaaac acggcattcg ccatgacacc acggtcattc
    14761 tgtacgggcg tgacgtatac gctggagcgc gtgtggcgca aattatgctc tacgcaggcg
    14821 tgaaagatgt gcgtctgctg gatggcggct ggcaaacctg gtccgacgcg ggactacctg
    14881 ttgagcgagg aacgccaccg aaagtgaaag cggaaccgga tttcggcgtg aagctcccgg
    14941 cacaaccgca gttgatgctc gatatggaac aggcccgtgg actgctgcat cgccaggatg
    15001 catcgctggt gagcattcgt tcgtggccag aatttatcgg tacgaccagc ggttacagct
    15061 atattaaacc aaaaggtgaa atagccggag cacgttgggg acacgctggt agcgactcga
    15121 cgcatatgga agattttcat aacccggatg gcaccatgcg cagcgccgat gatattaccg
    15181 ctatgtggaa agcgtggaat atcaaaccag agcagcaa
//
LOCUS       DAJTVE010000101        15019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000101 DAJTVE010000000
VERSION     DAJTVE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..713
                     /locus_tag="OO439_002976"
     CDS             <1..713
                     /locus_tag="OO439_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317286.1"
                     /translation="LHQKHKLNAAARLQYEQARNKVIKKMAENIPEILVDELKNADIN
                     RRVNTLELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELAL
                     RITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSG
                     TVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLR
                     ALHHLRVQSTSKTAPQAK"
     gene            complement(916..1269)
                     /locus_tag="OO439_002977"
     CDS             complement(916..1269)
                     /locus_tag="OO439_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6317287.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1407..3053)
                     /gene="groL"
                     /locus_tag="OO439_002978"
     CDS             complement(1407..3053)
                     /gene="groL"
                     /locus_tag="OO439_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6317288.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3097..3390)
                     /locus_tag="OO439_002979"
     CDS             complement(3097..3390)
                     /locus_tag="OO439_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6317289.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3666..4922
                     /gene="yjeH"
                     /locus_tag="OO439_002980"
     CDS             3666..4922
                     /gene="yjeH"
                     /locus_tag="OO439_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6317290.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4944..5414)
                     /gene="fxsA"
                     /locus_tag="OO439_002981"
     CDS             complement(4944..5414)
                     /gene="fxsA"
                     /locus_tag="OO439_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6317291.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5751..7187
                     /gene="aspA"
                     /locus_tag="OO439_002982"
     CDS             5751..7187
                     /gene="aspA"
                     /locus_tag="OO439_002982"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6317292.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7305..8606
                     /gene="dcuA"
                     /locus_tag="OO439_002983"
     CDS             7305..8606
                     /gene="dcuA"
                     /locus_tag="OO439_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6317293.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8722..9060
                     /gene="cutA"
                     /locus_tag="OO439_002984"
     CDS             8722..9060
                     /gene="cutA"
                     /locus_tag="OO439_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6317294.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9036..10733
                     /gene="dsbD"
                     /locus_tag="OO439_002985"
     CDS             9036..10733
                     /gene="dsbD"
                     /locus_tag="OO439_002985"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6317295.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10770..11345
                     /locus_tag="OO439_002986"
     CDS             10770..11345
                     /locus_tag="OO439_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6317296.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11452..11527
                     /locus_tag="OO439_002987"
     tRNA            11452..11527
                     /locus_tag="OO439_002987"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11485..11487,aa:Phe,seq:gaa)
     gene            12144..13681
                     /gene="cadC"
                     /locus_tag="OO439_002988"
                     /pseudo
     CDS             12144..13681
                     /gene="cadC"
                     /locus_tag="OO439_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14046..>15019
                     /gene="cadB"
                     /locus_tag="OO439_002989"
     CDS             14046..>15019
                     /gene="cadB"
                     /locus_tag="OO439_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6317297.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLL"
BASE COUNT         3692 a         3751 c         3600 g         3976 t
ORIGIN      
        1 gtctgcatca aaagcataaa ctgaatgctg ctgcacgcct tcagtatgag caagctcgca
       61 ataaagtcat taagaaaatg gcagaaaaca tcccagaaat tctggttgat gagcttaaaa
      121 atgccgatat taatcgtcgg gttaacacgc tggaacttac cgataatcaa ggcgaaaatc
      181 ttaccttcgt attaacgcta catgacggta gcaaatgtga gttagtcgtc aatgagttgc
      241 agattgaaat gctggcgcga gcaatcattc atgcaattaa taatgcggaa atgcgtgaac
      301 tggcattacg tattacctca ctgttagatt tcctgccgtt atacgacgtt gactgccagg
      361 acaatggcaa tctggaatat gacacctatt cccaaccgga atggaaacat aacctgttcg
      421 accattatct ggcggtgctt taccgcttta aagatgaaag tggcaaagaa caatttagcg
      481 gcacagtggt gaaaacgcgt gaagcaacgc cgggtaaaga aatagaggcg atcactcgcc
      541 gaatgcttga tttcagccca cgactgaaaa aactcgcggg tgtgccatgt caggtctatg
      601 ttcgcaccgt agcggcgaat aacgcccaac cgctaaccca ggatcaatgc ttacgcgcgc
      661 tgcatcactt acgtgttcaa tccaccagca aaactgcacc acaggcgaaa taatctcatc
      721 tttgctaatg cgaggctgga gccatccagc ctgaaatgac tgataacgtg atcgttggtc
      781 atgccggatg cgacgtaaac gccttatccg gcctacaaat tctcaagcat tcaatcagtt
      841 gcaatgcctg ctgtaggcct gataagcgaa gcgcatcagg caataatacg tttgtcaccc
      901 agcctctgga tcacatcagt tcgggcactt ataaacctga ccgataatct ggctatccgt
      961 cgggacaaaa ctggacaaca ttccctgcga cgggctgctg atgccataaa tcacgttacc
     1021 gcccattgca gccgcctggt tgcgcagatc gtttgctgcg ccgcgcatag aaccgccctc
     1081 ttctccgtgt tgcccggaaa gccagttgct ttgcttacct gtcgcagtac caatcagctg
     1141 gcactctgcg cctggctgct cgtccacaat gcgtacactc tgaccggcag cactcaattc
     1201 gttgctggag ctacaacccg ccatcagtag cgcggcaccg acaatccctg ctaagtattt
     1261 tacgtgcatg ttattcccca taatcaatga gctggacgcc actcgcgtcg tccgtgtctg
     1321 aatcttatac tagaaagatg accaaaagaa aaacccccag acatttctgc ccgggggttt
     1381 gtttatttct gcgaggtgca gggcaattac atcatgccgc ccatgccacc catgccgccc
     1441 ataccgccag cagcgcctaa gtcagctgca tcatttttcg gcaggtcggt aaccatgcac
     1501 tcggtggtga tcatcaggcc agccacagaa gccgcgtact gcagagcaga acgggttact
     1561 ttggttgggt ccaggatacc catgtcgatc atgttgccgt attcttcggt tgctgcgttg
     1621 taaccgtagt tgccgtcgcc gcctttaacg gtgttagcaa caacagacgg ttcttcgccg
     1681 cagttcagga cgatctgacg cagcggagct tccattgcac gcagtgcaac tttgataccc
     1741 acgttctggt cttcgttctg accacgcagg tcagccagtt tagacgctac gcggatcagc
     1801 gcaacaccac caccagcaac cacgccttct tctaccgcag cacgggtcgc gtgcagggcg
     1861 tcttcaacgc gtgctttttt ctctttcatt tcaacttcgg tagcagcacc tactttgata
     1921 actgcaacgc cgcctgccag tttcgctacg cgttcctgca gtttttcacg gtcgtagtca
     1981 gaagttgctt cttcaatctg ctgacggatc tgagcaacac ggccctggat tgcagcttct
     2041 tcacccacgc catcgatgat agtggtggtg tctttgttga tcacaacacg tttagcctga
     2101 cccaggtctt ccagggttgc tttttccagt tccataccga tctcttcaga gatcacggta
     2161 ccgccagtca gggttgcgat atcctgcagc atagctttac gacgatcgcc gaagcccgga
     2221 gctttaactg cagcaacttt cacgatgcca cgcatggtgt taacaaccag agttgccagc
     2281 gcttcgcctt ctacatcttc agcgatgatc agcagcggtt tgcctgcttt ggcaacggct
     2341 tccagaaccg gcagcatttc gcggatgttg gagattttct tgtcagccag caggatgaac
     2401 gggctttcca gttctactgc gccagtttcc ggcttgttga tgaagtaagg agacaggtag
     2461 ccacggtcga actgcatacc ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct
     2521 tcaacggtga taacgccttc tttaccgact ttgtccatcg cttcagcgat cagtttacct
     2581 acggtttcgt cggagttagc ggagatggta ccaacctgag caatcgcttt agagtcagag
     2641 cacggtacgg acagcgcttt cagttcttca actgcagcgg taacggcttt gtcgatacca
     2701 cgtttcaggt ccatcgggtt catgcccgca gcaacagctt tcagaccctc agtgatgata
     2761 gcctgagcca gtacagttgc agtggtggta ccgtcgcctg cagcgtcgtt cgctttagag
     2821 gcaacttctt tcaccatctg cgcacccatg ttttcgaact tgtcttccag ttcgatttca
     2881 cgagcaacgg aaacaccatc tttggtgatg gtcggtgcac cgaaagattt atccagaact
     2941 acgttacggc ctttcggacc gagggtaact ttcactgcat ctgccagtac gtttacgccg
     3001 cgcagcattt tcacacgagc gtcgttaccg aattttacgt ctttagctgc cattatcttt
     3061 attccttaaa ttcgtatgtt cagtgtcgtg cgaggattac gcttcaacaa ttgccagaat
     3121 gtcgctttcg gacatgatca acacttcttc attgtcgatc ttctcagatt tcacaccgta
     3181 gccatcgttg aaaataacga tgtcgccaac tttcacatcc agcggcttca cttcgccatt
     3241 ttcaaggata cggccattgc cgacagccag cacttcgccg cgggtggatt tagccgctgc
     3301 agagccggtc agaacgatgc cgccagcaga tttagtttca acttctttac gcttgacgat
     3361 cacgcgatca tgcaatggac gaatattcat tgataactct cctttgagaa agtccgtatc
     3421 tgttatgggt gacgccggag cttacgtggt ttcccggctg gtgaccagag aaatggggat
     3481 gaggattcgc cccttcaagg gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt
     3541 gaaaaccctg attcacctca catttcatcg caatttcttc atcgccgtca taagcgaatc
     3601 tgattgtgct accatcgaaa atctacgcag ttgcccaaaa tttgggcgca atcggatatc
     3661 aacccatgag tggactcaaa caagaactgg ggctggccca gggcattggc ctgctatcga
     3721 cgtcattatt aggcaccggc gtgtttgccg ttcctgcgtt agctgcacag gtagcaggca
     3781 ataacagcct gtgggcgtgg cccgttttga ttatcttagt gttcccgatt gcgattgtgt
     3841 ttgcgattct gggtcgccac tatcccagcg caggcggtgt cgcgcacttc gtcggtatgg
     3901 cgtttggttc gcggcttgag cgagtcaccg gctggttgtt tttatcggtc attcccgtgg
     3961 gtttgcctgc cgcgctacaa attgccgccg ggttcggcca ggcaatgttt ggctggcata
     4021 gctggcaact gttgttggca gaactcggta cgctggcgct ggtgtggtat atcggtactc
     4081 gcggtgccag ttccagtgct aatctacaaa ccgttattgc cggacttatc gtcgcgctga
     4141 ttgtcgctat ctggtgggcg ggcgatatca aacctgcgaa tatccccttc ccggcacctg
     4201 gtaatatcga acttaccggg ttatttgctg cgttatcagt gatgttctgg tgttttgtcg
     4261 gtctggaagc atttgcccat cttgcctcgg aatttaaaaa tccagagcgt gattttcctc
     4321 gcgcattgat gattggtctg ctgctggcag gattagtcta ctggggctgt acggtagtcg
     4381 tcttacactt cgacgcctat ggtgaacaaa tggccgcggc agcatcgctt ccaaaaattg
     4441 tagtgcaact gttcggtgta ggagcgttat ggattgcctg cgtaattggc tatctgacct
     4501 gctttgccag tctcaacatt tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc
     4561 aacataatcc tgaccactac ctggcacgcc tctcttctcg ccatatcccg aataatgccc
     4621 tcaatgcggt gctcggctgc tgcgtggtga gcactttggt gattcatgct ttagagatca
     4681 atctggacgc tcttattatt tatgccaatg gcatctttat tatgatttat ctgttatgca
     4741 tgctggcagg ctgtaaatta ttgcaaggac gttatcgact actggcggtg gttggcgggc
     4801 tgttatgcgt tctgttactg gcaatggtcg gctggaaaag tctctatgcg ctgattatgc
     4861 tggcggggtt atggctgttt ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat
     4921 aatccggcgt ttcgacatta atcctagcga tcgtctttat gatcaaggcg gtcgcggtca
     4981 tcatcctttc gctggtactc accatcaaaa gtattaccgc caccggtccc ggcgctaaaa
     5041 ccgccgcctg gcatgcgaga aaagcgcaaa tgtggcatca acttcactgt cagatgcttt
     5101 tgcaccggcg gcaataaaag cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa
     5161 agcagcaaac cggcaataat cagcgaaaca cttttaatca tctccgccgc agggttttca
     5221 cccgccgcca ttttttgctg catcagcacg aaattcttaa agccctggtt acgtaccagt
     5281 gacataccga taactgacgt gaatataacc agcacgaggg tcagcaatac ccccaataca
     5341 tgggcaacct gaataaagat tgaaatctca atatagacat aaaggaaaat ggcaataaaa
     5401 ggtaaccagc gcaaaggttt ctcctgtaat agcagccggt taaacccggc tacctgaatg
     5461 ggttgcgaat cgcgttcagc ttatattgtg gtcattagca aaatttcaag atgtttgcgc
     5521 aactattttt ggtagtaatc ccaaagcggt gatctatttc acaaattaat aattaagggg
     5581 taaaaaccga cacttaaagt gatccagatt acggtagaaa tcctcaagca gcatatgatc
     5641 tcgggtattc ggtcgatgca ggggataatc gtcggtcgaa aaacattcga aaccacatat
     5701 attctgtgtg tttaaagcaa atcattggca gcttgaaaaa gaaggttcac atgtcaaaca
     5761 acattcgtat cgaagaagat ctgttgggta ccagggaagt tccagctgat gcctactatg
     5821 gtgttcacac tctgagagcg attgaaaact tctatatcag caacaacaaa atcagtgata
     5881 ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag
     5941 agctgcaaac cattcctaaa agtgtagcga atgccatcat tgccgcatgt gatgaagtcc
     6001 tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt ctaccagggc ggcgcaggta
     6061 cttccgtaaa catgaacacc aacgaagtgc tggccaatat cggtctggaa ctgatgggtc
     6121 accagaaagg tgaatatcag tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca
     6181 ctaacgacgc ctacccgacc ggtttccgta tcgcagttta ctcttccctg attaagctgg
     6241 tagatgcgat taaccaactg cgtgaaggct ttgaacgtaa agctgtcgaa ttccaggaca
     6301 tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc gatgaccctc ggtcaggaat
     6361 tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt accgctgaac
     6421 tgctgctgga agttaacctt ggcgcaacag caatcggtac tggtctgaac acgccgaaag
     6481 agtactctcc gctggcagtg aaaaaactgg ctgaagtcac tggcttccca tgcgtaccgg
     6541 ctgaagacct gatcgaagcg acctctgact gcggcgctta tgttatggtt cacggcgcgc
     6601 tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg
     6661 gtccacgtgc cggcctgaac gagatcaacc tgccggaact gcaggcgggc tcttccatca
     6721 tgccagctaa agtaaacccg gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca
     6781 tcggtaacga caccactgtt accatggcag cagaagcagg tcagctgcag ttgaacgtta
     6841 tggagccggt cattggccag gctatgttcg aatccgttca cattctgacc aacgcttgct
     6901 acaacctgct ggaaaaatgc attaacggca tcactgctaa caaagaagtg tgcgaaggtt
     6961 acgtttacaa ctctatcggt atcgttactt acctgaaccc gttcatcggt caccacaacg
     7021 gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg
     7081 aacgcggtct gttgactgaa gcggaacttg acgatatttt ctccgtacag aatctgatgc
     7141 acccggctta caaagcaaaa cgctatactg atgaaagcga acagtaatcg tacagggtag
     7201 tacaaataaa gaaggcacgt cagatgacgt gccttttttc ttgtgagcag taacttaaaa
     7261 ataacaatct aatatcaact tgttaaaaaa caaggaaggc taatatgcta gttgtagaac
     7321 tcatcatagt tttgctggcg atcttcttgg gcgccagatt ggggggaata ggtattggtt
     7381 ttgcaggcgg attgggggtg ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc
     7441 cgttcgatgt catttccatt atcatggcgg ttatcgccgc tatttctgcc atgcaggttg
     7501 ctggcggtct ggactatctg gttcatcaga cagaaaagct gctgcgccgt aacccgaaat
     7561 acatcacaat cctcgcaccg atcgtgacct atttcctgac aatctttgct ggtactggca
     7621 acatctctct ggcgacactg ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac
     7681 cttgccgtcc gctgtctact gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa
     7741 tctcagcggc agtggtttac atgtcttccg tgatggaagg tcatggcatc agctacctcc
     7801 atctgctctc cgtggtcatc ccgtccaccc tgctggcggt tctggtgatg tccttcctgg
     7861 tcactatgct gttcaactcc aaactctctg acgatccgat ttatcgcaag cgtctggaag
     7921 agggcctggt tgaactgcgc ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt
     7981 ccgtctggct gttcctgctg ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc
     8041 caagcatggg tctggttgaa aaaccgctga tgaacaccac caacgcaatc ctgatcatca
     8101 tgctcagcgt tgcaactctg accaccgtta tctgtaaagt cgataccgac aacatcctca
     8161 actccagcac cttcaaagca ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc
     8221 tgggcgatac tttcgtttcc aacaacatcg actggatcaa agataccgct ggtgaagtga
     8281 ttcagggtca tccgtggctg ctggccgtca tcttcttctt tgcttctgct ctgctgtact
     8341 ctcaggctgc aaccgcaaaa gcactgatgc cgatggctct ggcactgaac gtttctccgc
     8401 tgaccgctgt tgcttctttc gctgcggtgt ctggtctgtt cattctgccg acctatccga
     8461 cgctggttgc tgcggtacag atggatgaca cgggtacaac ccgtatcggt aaattcgtct
     8521 tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc cctggccgtt tgcttcggct
     8581 tcgtgctggg tagcttcatg ctataatgac caatcgcggg gcgttcacgc cccgctttct
     8641 ttcccgccga ctaacatcct tcccccgtcc gttgtatagt gacctctctc ttgcggttcc
     8701 atctgttctt gcgaggtgtt tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg
     8761 gtgctatgta ccgcaccgga tgaagcgaca gcccaggatt tagccgccaa agtgctggcg
     8821 gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta cctctctcta ttactgggaa
     8881 ggtaagctgg agcaagaata cgaagtgcag atgattttaa aaactaccgt atctcaccag
     8941 caggcactgc tggaatgcct gaagtctcat catccatatc aaaccccgga acttctggtt
     9001 ttacctgtta cacacggaga cacagattac ctctcatggc tcaacgcatc tttacgctga
     9061 tcctgctact ttgcagcact tccgtttttg ccggattatt cgacgcgccg ggacgttcac
     9121 attttgtccc cgcggatcaa gcctttgctt ttgattttca gcaaaaccaa catgacctga
     9181 atctgacctg gcagatcaaa gacggttact acctctaccg taaacagatc cgcattacgc
     9241 cggaacacgc gaaaattgcc gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt
     9301 tttacggcaa aagcgagatt taccgcgatc ggctgacgct tcccgtaacc atcaaccagg
     9361 cgagtgcggg agcgacgtta actgtcacct accagggctg tgctgatgcc ggtttctgtt
     9421 atccgccaga aaccaaaacc gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac
     9481 agcctgtgtc tgttccgcag caagagcagc acaccgcgca attgcccttt tccgcgctct
     9541 gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg cgtgctgcca atgtacccac
     9601 tgatttctgg catcgtgctg ggtggtaaac agcggctctc cactgccaga gcattgttgc
     9661 tgacctttat ttatgtgcag gggatggcac tgacttacac ggcgctgggt ctggtagttg
     9721 ccgccgcagg gttacagttc caggcggcgc tacagcaccc atacgtgctc attggcctcg
     9781 ccatcgtctt taccttgctg gcgatgtcaa tgtttggctt gtttactcta caactcccct
     9841 cttcgctgca aacacatctc acattgatga gcaatcgcca acagggcggc tcacctggcg
     9901 gtgtgtttgt tatgggggcg attgccggac tgatctgttc accatgcacc accgcaccgc
     9961 ttagcgcgat tctgctgtat atcgcccaaa gcgggaacat gtggctgggc ggcggcatgc
    10021 tttatctcta tgcgttgggc atgggcctgc cgctgatgct aattaccgtc tttggtaacc
    10081 gcttgctgcc gaaaagcggc ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga
    10141 tcctcgcact gccgatcttc ctgctggagc gagtgattgg tgatatatgg ggattacgct
    10201 tgtggtcggc gctgggtgtc gcattctttg gctgggcctt tatcaccagc ctacaggcca
    10261 aacgcggctg gatgcgcgtg gtgcaaataa tcctgctggc agcggcattg gttagcgtgc
    10321 gcccacttca ggattgggca tttggtgcga cgcataccgc gcaaactcag acgcatctca
    10381 actttacaca aatcaaaacg gtagatgagt taaatcaggc gctcgttgaa gccaaaggca
    10441 aaccggtgat gttagatctt tatgccgact ggtgcgtcgc ctgtaaaaag tttgagaaat
    10501 acaccttcag cgacccgcag gtgcaaaaag cgttagcaga cacggtctta cttcaggcca
    10561 acgtcactgc taacgacgca caagatgtgg cgctgttaaa gcatcttaat gtccttggac
    10621 taccgacaat tctctttttt gacggacaag gccaggagca tccacaagca cgcgtcacgg
    10681 gctttatgga tgctgaaacc ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac
    10741 ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga agatgtactg ggagaagccc
    10801 tgaaattatt agaattacaa gggattgcca acaccacgct ggagatggtt gctgaacgtg
    10861 tggattatcc actggacgag ctacgccgct tctggccaga caaagaggca atcctctacg
    10921 atgcgctgcg ttatcttagc caacagatag atgtctggcg tcgtcagctg atgcttgacg
    10981 aaacgcaaac cgccgaacaa aagctgctgg cacgttatca ggcgttatcg gagtgcgtta
    11041 aaaacaaccg ctatccgggc tgtctattta tcgctgcctg tacgttttat cccgaccctg
    11101 gccaccctat tcatcaactg gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg
    11161 aactgttaac cacgctggaa gttgacgatc cggcgatggt agcaaagcag atggaactgg
    11221 tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag ccaggcggat gtcgacaccg
    11281 cacatcggct ggcggaagat atcctgcgct tcgcccgctg ccgccagggt ggtgcactga
    11341 cctgacagaa acacagaaaa gaagcgattt gccgcaatct taagcagttg aatcgctttt
    11401 actgaaatta ggttgacgag atgtgcagat tacggtttaa tgcgccccgt tgcccggata
    11461 gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg
    11521 ggcaccaaat tcatataaac ggacctccac ggaggtccgt ttttcgtttc aggacgccac
    11581 gatttaagcg ttctgcctcc aaatcaattc taccgaactc aaccagattc tccccacatc
    11641 accagcattt tgcgggtata tcccaattcg ggaaaatttg ttcctgagcg atagcactga
    11701 ctgacgtgaa atgtcgtgcg gccccgtgat gttgttgaac gtcaaatgac gtcatcagga
    11761 gcgtaacgca tccataaagc acaacaccgg tctgaacggc gactgatgag attttctgaa
    11821 tgagaacaaa gagaaatgta tcagtccgct tgctcatgca aagactaaca atccattaaa
    11881 atagtaagcg ctccggacaa ttttccatgg attattttct acacattttt ctttggcaaa
    11941 gatgatgaat tttgatggta aggaatatta cttctggttc tcagtaaaat cctttcgtaa
    12001 tactatgtaa tcaagaagtt tatggctggt aaaaataacg tcttgcattc accaataaca
    12061 tgtaaataaa cccatctatt gatggtaaaa ataggtcatg gcaattatca ttgcaccatt
    12121 cccttttcga atgagtttct attatgcaac aacctgtagt tcgcgttggc gaatggcttg
    12181 ttactccgtc cataaaccaa attagccgca atgggcgtca acttaccctt gagccgagat
    12241 taattgatct tctggttttc tttgctcaac acagtggcga agtacttagc agggatgaac
    12301 ttatcgataa tgtctggaag agaagtattg tcaccaatca cgttgtgacg cagagtatct
    12361 cagaactacg taagtcatta aaagataatg atgaagatag tcctgtctat atcgctactg
    12421 taccaaagcg cggctataaa tgaatggtgc cggttatctg gtacagcgaa gaagagggag
    12481 aggaaataat gctatcttcg cctcccccta taccagaggc ggttcctgac acaaattctc
    12541 cctcccacag tcttaccatt caaaacaccg caacgccacc tgaacaatcc ccagttaaaa
    12601 gcaaacgatt cactaccttt tgggtatggt ttttttcctg ttgtcgttag gtatctgtgt
    12661 agcactggta gcgttttcaa ctcttgatac acgtcttcct atgagcaaat cgcgtatttt
    12721 gctcaatcca cgcgatattg acattaatat ggtaaataaa agttgtaaca gctggagttc
    12781 cccgtatcag ctctcttacg cgataggcgt gggtgatttg gtggcgacat cacttaacac
    12841 cttctccacc tttatggtgc atgacaaaat caactacaac attgatgaac cgagcagttc
    12901 cggtaaaaca ttatctattg cctttgttaa tcagcgccaa taccgtgctc aacaatgctt
    12961 tatgtcggta aaattagtag acaatgcaga tggttcaacc atgctggata aacgttatgt
    13021 catcactaac ggtaatcagc tggcgattca aaatgattta ctggagagtt tatcaaaagc
    13081 gttaaaccaa ccgtggccac aacgaatgca ggagacgctc cagcaaattt tgcctcatcg
    13141 tggtgcgtta ttaactaatt tttatcaggc tcatgattat ttactgcatg gcgatgacaa
    13201 atcattgaat cgtgccagtg aattattagg tgagattgtt caatcatccc cagaatttac
    13261 ctacgcgaga gcagaaaaag cattagttga tatcgtgcgc cattctcaac accctttaga
    13321 tgaaaaacaa ttagcagcac tgaacacaga aatagacaac attgttacac tgccggaatt
    13381 gaacaacctg tccattatat atcaaataaa agcggtcagt gctctggtaa aaggtaaaat
    13441 agatgagtct taccaggcga taaatactgg cattgatctt gaaatgtcct ggctaaatta
    13501 tgtgttgctt ggcaaggttt atgaaatgaa ggggatgaac cgggaagcag ctgatgcata
    13561 tctcaccgcc tttaatttac gcccaggggc aaacaccctt tactggattg aaaatggtat
    13621 tttccagact tctgttcctt atgttgtacc ttatctcgac aaatttctcg cttcagaata
    13681 agtaactccg ggttgattta tgctctggaa tatttgttgt tgagtttttg tatgttccca
    13741 ttggtataat atgttgcggc aatttatttg ccgcataatt tttattacat aaatttaacc
    13801 agagaatgtc atgcaatcca ttgtaaacat tgaatgttta tcttttcatg atatcaactt
    13861 gcgatcctga tgtgttaata aaaaacctca agttctcact tacagaaact tttgtgttat
    13921 ttcacctaat ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct
    13981 ggtcaggaaa tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga
    14041 agagcatgag ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta
    14101 atatgatggg gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg
    14161 ctatctgggg ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc
    14221 gactggcaac aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttctc
    14281 ctgcatttgg ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctag
    14341 cgattggtat taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg
    14401 ttccggcggg tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg
    14461 gcggtacctg ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg
    14521 tgatgactgc tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga
    14581 atactgcgga taccactgat ggtcatgcga tcattaaaag tattctgctc tacctgtggg
    14641 ccttcgtggg tgttgagtcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta
    14701 ccgttccgct ggcaaccatg ctgggtactg gtttagcagg tattgtttac atcgctgcga
    14761 ctcaggtgct ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg
    14821 caatcagtgc ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg
    14881 cctttgcgtg cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac
    14941 gtgccgctaa cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc
    15001 cgaaaaaagg tctgctgct
//
LOCUS       DAJTVE010000102        14965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000102 DAJTVE010000000
VERSION     DAJTVE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO439_002990"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO439_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6317298.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO439_002991"
     CDS             complement(1608..1931)
                     /locus_tag="OO439_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6317299.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO439_002992"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO439_002992"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6317300.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO439_002993"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO439_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6317301.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO439_002994"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO439_002994"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6317302.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO439_002995"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO439_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6317303.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO439_002996"
     CDS             7531..8568
                     /locus_tag="OO439_002996"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6317304.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO439_002997"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO439_002997"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6317305.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO439_002998"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO439_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6317306.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO439_002999"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO439_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6317307.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO439_003000"
     CDS             10739..11620
                     /locus_tag="OO439_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317308.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO439_003001"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO439_003001"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6317309.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO439_003002"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO439_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6317310.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OO439_003003"
     CDS             complement(14017..>14965)
                     /locus_tag="OO439_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6317311.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJTVE010000103        14404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000103 DAJTVE010000000
VERSION     DAJTVE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO439_003004"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO439_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6317312.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO439_003005"
     CDS             1828..3147
                     /locus_tag="OO439_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6317313.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OO439_003006"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OO439_003006"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6317314.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OO439_003007"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OO439_003007"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6317315.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OO439_003008"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OO439_003008"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6317316.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OO439_003009"
     CDS             8179..9729
                     /locus_tag="OO439_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6317317.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OO439_003010"
     CDS             9731..10753
                     /locus_tag="OO439_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6317318.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OO439_003011"
     CDS             10750..11646
                     /locus_tag="OO439_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6317319.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OO439_003012"
     CDS             11643..12629
                     /locus_tag="OO439_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6317320.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OO439_003013"
     CDS             12658..13548
                     /locus_tag="OO439_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6317321.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO439_003014"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO439_003014"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6317322.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3557 c         3630 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJTVE010000104        14297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000104 DAJTVE010000000
VERSION     DAJTVE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..624)
                     /locus_tag="OO439_003015"
     CDS             complement(<1..624)
                     /locus_tag="OO439_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6317323.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGAS"
     gene            880..1035
                     /gene="rseD"
                     /locus_tag="OO439_003016"
     CDS             880..1035
                     /gene="rseD"
                     /locus_tag="OO439_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6317324.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1032..1607
                     /gene="rpoE"
                     /locus_tag="OO439_003017"
     CDS             1032..1607
                     /gene="rpoE"
                     /locus_tag="OO439_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6317325.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1640..2290
                     /gene="rseA"
                     /locus_tag="OO439_003018"
     CDS             1640..2290
                     /gene="rseA"
                     /locus_tag="OO439_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6317326.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2290..3246
                     /gene="rseB"
                     /locus_tag="OO439_003019"
     CDS             2290..3246
                     /gene="rseB"
                     /locus_tag="OO439_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6317327.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3243..3722
                     /gene="rseC"
                     /locus_tag="OO439_003020"
     CDS             3243..3722
                     /gene="rseC"
                     /locus_tag="OO439_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6317328.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3920..5719
                     /gene="lepA"
                     /locus_tag="OO439_003021"
     CDS             3920..5719
                     /gene="lepA"
                     /locus_tag="OO439_003021"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6317329.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5735..6709
                     /gene="lepB"
                     /locus_tag="OO439_003022"
     CDS             5735..6709
                     /gene="lepB"
                     /locus_tag="OO439_003022"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6317330.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            6982..7662
                     /gene="rnc"
                     /locus_tag="OO439_003023"
     CDS             6982..7662
                     /gene="rnc"
                     /locus_tag="OO439_003023"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6317331.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7659..8564
                     /gene="era"
                     /locus_tag="OO439_003024"
     CDS             7659..8564
                     /gene="era"
                     /locus_tag="OO439_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6317332.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8576..9304
                     /gene="recO"
                     /locus_tag="OO439_003025"
     CDS             8576..9304
                     /gene="recO"
                     /locus_tag="OO439_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6317333.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9316..10047
                     /gene="pdxJ"
                     /locus_tag="OO439_003026"
     CDS             9316..10047
                     /gene="pdxJ"
                     /locus_tag="OO439_003026"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6317334.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10047..10427
                     /locus_tag="OO439_003027"
     CDS             10047..10427
                     /locus_tag="OO439_003027"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6317335.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10649..10729
                     /gene="shoB"
                     /locus_tag="OO439_003028"
     CDS             10649..10729
                     /gene="shoB"
                     /locus_tag="OO439_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6317336.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10923..11183)
                     /gene="yfhL"
                     /locus_tag="OO439_003029"
     CDS             complement(10923..11183)
                     /gene="yfhL"
                     /locus_tag="OO439_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6317337.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11398..12588
                     /locus_tag="OO439_003030"
                     /pseudo
     CDS             11398..12588
                     /locus_tag="OO439_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12773..13960)
                     /locus_tag="OO439_003031"
     CDS             complement(12773..13960)
                     /locus_tag="OO439_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6317338.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(13960..14256)
                     /locus_tag="OO439_003032"
     CDS             complement(13960..14256)
                     /locus_tag="OO439_003032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317339.1"
                     /translation="MKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNKK
                     LVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT         3603 a         3584 c         3690 g         3420 t
ORIGIN      
        1 cgacgcaccg ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt
       61 atttcggttc catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc
      121 agagacaatc agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg
      181 cgccttgctc accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat
      241 acgacggtga ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg
      301 aacgtgggta tcaaacaaaa ccccctggtc aattaaccat tgcacacagg aacgagcatt
      361 gctggcgaca aattcaactg catggcgatc gcaaatacca gccccggcaa tcaatgtgtc
      421 ttccacatgc gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc
      481 ataaaatgtt gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc
      541 agccaggcgt agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca
      601 tgaatgttca gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcag
      661 catagcatca tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa
      721 cacgttgacg cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt
      781 gaaataaaaa ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata
      841 atgcgatgtt cagattctgt agacttataa tgatagataa tgatccgtct acagcatgac
      901 aaacaaaaac agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg
      961 ctcaaattgc agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga
     1021 ctttacctcg gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg
     1081 gagatcagaa agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg
     1141 tttcccgcta tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag
     1201 cctatcgtgc gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga
     1261 ttgctgtaaa tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg
     1321 atgtggatgc cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga
     1381 accctgagaa cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt
     1441 ccctcccgga agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg
     1501 aagagatagc cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag
     1561 cgagggaagc tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata
     1621 ctggataagg gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac
     1681 gctggatagt gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga
     1741 aagctatcac ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga
     1801 tatctcttca cgcgtgatgg ccgccattga agaagagcca gtacgtcaac cggcgacatt
     1861 gatcccggaa gcccagcctg cgccgcatca atggcacaaa atgccattct ggcagaaagt
     1921 acgtccgtgg gcggcacagc ttacccaaat gggcgtagca gcatgcgtat cgcttgcagt
     1981 tatcgttggc gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc
     2041 ggtatttaat acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc
     2101 tgaagcgacc gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa
     2161 tgcaatgttg caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga
     2221 gcaggcgcaa acccagcaag ccgctgtaca ggtgccagga cttcaaactt taggaacgca
     2281 atcgcagtaa tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc
     2341 tctgctaacg cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc
     2401 agtcagtcac tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct
     2461 ctgcgttatc gacatgcacg cctcgataac cgtcctcttg cacaattgtt gcaaatggat
     2521 ggcccgcgcc gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt
     2581 gaaccgttca cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatacc
     2641 gatttcaaac gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct
     2701 gatcgtcttt gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc
     2761 gtgtggatgg acactgaatc gaaattaccg atgagggttg atctccttga tcgcgatggt
     2821 gaaacgctgg aacaatttcg cgtgatttct tttaacgtca atcaggatat cagcagcagt
     2881 atgcagacgc tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa
     2941 gctaaattca gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt
     3001 cgacgtccgc taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga
     3061 ttattcagct tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg
     3121 cgcaccggac gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc
     3181 ggtgaactgc cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg
     3241 caatgatcaa agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt
     3301 gtgatgttaa agcctcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt
     3361 tgaataaact tggcccgcaa accacgcata ccattgtcgt accctgtgat gaaccgttag
     3421 tgccggggca aaaagtggaa ttggggatcg ccgaaggcag cctgcttagc tccgcattac
     3481 tggtttatat gtcgccgctg gtgggattat tccttatcgc ttcgctattt cagctactct
     3541 ttgcttctga cgttgcagca ttatgtggtg cgatccttgg cggtattggc gggtttctga
     3601 ttgcgcgcgg ttactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatattaa
     3661 gcgtggcatt gccgcctgac cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt
     3721 gatttcactg gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca
     3781 atccatacgg tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta
     3841 ttaatacaga cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa
     3901 ggcacaataa tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga
     3961 ccacggtaaa tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg
     4021 tgaaatggag gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat
     4081 caaagcgcaa agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa
     4141 ctttatcgac accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc
     4201 ctgtgaaggt gcattgctgg tggtcgacgc cgggcagggc gtggaagcgc aaaccctggc
     4261 aaactgctac accgccatgg aaatggatct cgaagtggtg ccagtactga acaagattga
     4321 cctgccggca gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga
     4381 cgccactgac gcggtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga
     4441 acgtctggtg cgcgacattc cgccgccgga aggcgatccg gaaggctcgt tgcaggcact
     4501 aattatcgac tcatggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa
     4561 cggcaccctg cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc
     4621 cgaccgtctg ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga
     4681 agtaggctgg ctggtatgtg cgattaaaga tatccatggc gctccagtcg gcgatacctt
     4741 aacgctggcg cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca
     4801 ggtatacgcc ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct
     4861 gggtaaactc agcctgaatg atgcctcact gttctatgag ccggaaagct ccagcgcgct
     4921 gggctttggt ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggaacg
     4981 tctggaacgt gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt
     5041 tgaaaccacg tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa
     5101 taacatttac gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata
     5161 tctcggcaac gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta
     5221 ccacggtaat caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt
     5281 cttcgatcgc ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg
     5341 cttccaggcg tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc
     5401 gttggcgctg atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa
     5461 gatgaaagat ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac
     5521 gcacattatt gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta
     5581 tggcggcgat atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg
     5641 catgaagcag atcggtaacg tcgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt
     5701 cggcaaagac aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct
     5761 ggtgattgcc acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc
     5821 taaacggcgg gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc
     5881 aacgttgaaa aaggttgcac cgaagcttgg ctggctggaa accggagctt ctgtttttcc
     5941 ggtgctggct atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc
     6001 aggttcgatg atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta
     6061 tggcattaaa gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg
     6121 cgatatcgtg gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt
     6181 gggtttaccg ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc
     6241 gggatgcagt tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga
     6301 accgagcgat ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt
     6361 ctttgaagtg ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga
     6421 gacactgggt gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg
     6481 gatgtattac cagcagccag ggcaacaact ggcaacctgg attgttccgc cgggacaata
     6541 cttcatgatg ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc
     6601 tgaagcgaat ctggtcggtc gggcaacggc tatctggatg agtttcgata agcaagaagg
     6661 cgaatggccg acaggtgtgc gcttaagtcg cattggcggc atccattaat agcccatctt
     6721 cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt tatgagataa atctcccgtg
     6781 gctaacgaca tcccccgtcg ttgtgtatag aatattcccc cgaagtttaa ggttggcacc
     6841 tccaggttgc cacggcacac gaaacagcgt tggtccccat ataccggtaa actgaaactg
     6901 cagcgaagca gttagcagag ccatgtatat caggtctgtt tcgtgtgctg aattgttgac
     6961 gcatttattt attggtatcg catgaacccc atcgtaatta atcggcttca acggaagctg
     7021 ggctacactt ttaatcatca ggaactgttg cagcaggcat taactcatcg tagtgccagc
     7081 agcaaacata acgagcgttt agaattttta ggcgactcta ttctgagcta cgttatcgcc
     7141 aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg atatgagccg gatgcgcgcc
     7201 acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg aatttgagtt aggcgagtgc
     7261 ttacgtttag ggccaggtga acttaaaagc ggtggatttc gtcgtgagtc aattctcgcc
     7321 gataccgtcg aagcattaat tggtggcgta ttcctcgaca gtgatattca aaccgtcgag
     7381 aaattaatcc tcaactggta tcaaactcgt ttggacgaaa ttagcccagg cgataaacaa
     7441 aaagatccga aaacgcgctt gcaagaatat ttgcagggtc gccatctgcc gctgccgact
     7501 tatctggtag tccaggtacg tggcgaagcg cacgatcagg aatttactat ccactgccag
     7561 gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa gccgtcgtaa ggctgagcag
     7621 gctgccgccg aacaggcgtt gaaaaaactg gagctggaat gagcatcgat aaaagttact
     7681 gcggatttat tgccatcgtc ggacgtccga acgttggcaa atccacattg ttgaacaaac
     7741 tgctggggca gaagatctcc atcacctccc gcaaagcgca aacaactcgt caccgcattg
     7801 tggggatcca tactgaaggc gcgtatcagg cgatctacgt tgatacccct ggcctgcata
     7861 tggaagaaaa acgcgctatt aaccgcctga tgaacaaagc ggcgagcagc tctattggcg
     7921 acgttgagct ggtgattttt gtcgttgaag gcacccgctg gacgccggac gacgaaatgg
     7981 tgctcaacaa actgcgcgat ggcaaagcgc cggtgatcct cgcggtgaac aaagtggata
     8041 acgtgcagga aaaagccgat ctgctgccgc acttacagtt cctggcaagt cagatgaact
     8101 tcctcgatat cgtgccgatc tctgctgaaa ccgggctgaa tgtcgacact atcgcagcaa
     8161 tcgtgcgtaa gcatctgcct gaagcgaccc atcacttccc ggaagattac atcaccgatc
     8221 gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa actgatgcgt ttcctcggcg
     8281 ctgaactgcc gtactccgtg accgtggaga tcgaacgttt cgtctctaac gaacgcggtg
     8341 gttatgacat caatggtttg attctcgttg agcgtgaagg gcagaagaag atggttattg
     8401 gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc gcgtaaagac atgcaggaaa
     8461 tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt gaaatccggt tgggccgacg
     8521 acgaacgcgc actgcgcagt ctcggttacg ttgacgatct ttaagagtca ctccgatgga
     8581 aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg agcgaaacca gcctgatgct
     8641 ggacgtcttc acggaggaat cgggtcgcgt gcgtctggtt gccaaaggcg cacgctctaa
     8701 acgctctact ctgaaaggtg cattacagcc tttcacccct ctcttgctac gtttcggcgg
     8761 gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc tcgctggcgc tgccattaag
     8821 cggtatcacg ctttacagcg gtctgtacat caacgaactt ctctcccgcg tactggaata
     8881 cgagacgcgc ttctctgaac tctttttcga ttacttgcac tgcattcagt ctcttgcagg
     8941 tgccactggt acgccagaac ccgcgctgcg ccgctttgaa ctggcactgc tcgggcatct
     9001 gggctatggc gtcaatttta cccattgcgc gggtagcggc gagccggtag atgacaccat
     9061 gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc gtcgttatcg acaataaaac
     9121 gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa tttcctgacg cagacacact
     9181 acgcgccgcg aaacgcttta cccgcatggc gcttaagccg tatcttggcg gtaaaccatt
     9241 aaagagcagg gaactgttcc ggcagtttat gcctaagcga acggtgaaaa cacattatga
     9301 atgatgagga ttgtcatggc tgaattactg ttaggcgtca acattgacca tattgcaacg
     9361 ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc aggcggcgtt tattgccgag
     9421 caggcgggag cggacggcat taccgtgcat ttacgtgaag atcgccgcca cattactgac
     9481 cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa tgaatctgga gatggcagtg
     9541 accgaagaga tgctggcgat cgccgttgag acgaagccac atttttgctg cctggtaccg
     9601 gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg ttgcagggca gcgtgaaaaa
     9661 atgcgcgatg cctgcaaacg tctgacagat gccgggattc agatttctct ctttatcgac
     9721 gccgatgaag agcagatcaa agctgcggca gaggttggcg caccgtttat cgagatccac
     9781 accggttgct atgctgatgc caaaactgac gccgaacagg cgcaagagct ggtgcgtatc
     9841 gccaaagccg cgacctttgc cgctagtctc ggtctgaaag ttaacgccgg acacggtctg
     9901 acctatcaca acgtgaaagc cattgccgcc atccctgaga tgcatgaact gaatatcggt
     9961 catgccatta ttggccgtgc agtgatgacc ggactgaaag atgcggtggc agaaatgaag
    10021 cgtctgatgc tggaagcgcg tggctaatgg caatattagg tttaggcacg gatatcgtag
    10081 agatcgctcg catagaagca gtgatcgccc gatccggtga gcgcctggca cgccgcgtat
    10141 taagcgataa cgaatgggca atctggaaaa cgcaccacca gccggtgcgt tttctggcga
    10201 agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg caccgggatc cgcaatggtc
    10261 tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg caaaccacgg ctacggctat
    10321 ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt aaatatgcat gtaacgctgg
    10381 cagatgaacg ccactatgct tgtgccacgg taattattga aagttaaaat aataccgttc
    10441 tctttaaaaa gtaaaacccc cgaactggta aggaacgggg gttttacttt ttaactttaa
    10501 ttttgtgcag catgcaccct caacaacagc gattatggat cagtgtactt atcttttaca
    10561 agcaacttgc aatctttagc ataaaaactc gagcctttac gaagaaagca atactgaacg
    10621 gctgcattcc ttgtttaagg aaaaacgaat gactgattgc cgatacctga ttaaacgggt
    10681 catcaaaatc atcattgctg ttttacagct gatccttctg ttcttataac acaaggaaac
    10741 gtacttaagg tgcgtccggt gaaccaatcg gacgcgcctt taataactat aaataagtgt
    10801 cttggcagat actatataaa ttaacttagt gaatgattat gctaatgtca tcaattaaat
    10861 aaatataatg gcgttaaggc ttcccagtaa tataattaat actctacttc cagagtagaa
    10921 tattaaattt tatccgcgtg gtgcatcagc acaaatttat cccacaactg ttcttctgtc
    10981 tcgacatgcg ccggatcttt cacaatagta ttggggatcg ggcacacctt ctggcaggtt
    11041 ggtgtctcgt agtgccctac gcattcggta cacttatcgc tgttaatctc gtagatatga
    11101 tctcccattg aaatcgcctc attcgggcat tcgggttcac acatatcaca attaatacag
    11161 cgtttagtaa ttagtaaaga catttcaatg gattaccgtt aaatcatttt aaaatcagta
    11221 agttgtatcg agtttgtatg ctttactgtc attaacttac tgtatgttga tccagtgtat
    11281 ttaaccttga taaactcagt ccagcaacac aaaaccgcaa cacattgcat tttgtcccgt
    11341 agaaaagact tgtatgtgtg agcttgtttt ctgcgcctac gcagataagg attgagaatg
    11401 ccgcgcactg taacacataa tccggatagc cccaataatg acgatgtttt agccgcatct
    11461 gaaaaatggg acgcctgtaa acccccctat accagcgcac acatgaaaat ctgtgttgct
    11521 gccgccaaaa tcatcctcgc tgcttccggc gtggctcgcc gttccaaata cgaaaaagag
    11581 aactatctcc gtatcgattt cagcaaagcc ggtaaggtta cattttacgc cgagtttcca
    11641 aaaaagatgg gcctcaaagg taaaaagctc ggcgagtggc cggagctcgc tatccagctg
    11701 gcgcgcgaaa aagcactagg tatggctgac ggtggactgc gggcagagtc cgtacatgca
    11761 gcgctggaaa tgtaccggga tgacctcaaa gccaaagtag cccggcagaa gctgagcccg
    11821 gacagtttca caacctacgg ggtgcgtatc gaccggatta aagcaacgtt cggcgagcgt
    11881 gaggtgttca gcgacgtaac atacaatcgg ctggtggaag tgctggacga gtggatcgcc
    11941 actcgctcga acaataacgc cctggagttg tttgccgagc tccgtcggtt ctggaagttc
    12001 tgcgcaccta ctctttgcaa cggccgcaat gttgccgcca gtctgccaga tgattatgtt
    12061 tcctcccgcg tacagaaacc tacccccaca cggcttttta ccgatattga atcaatcgcc
    12121 cgactctggc tcaatgttgc tgcctgcacc tctgtacacc agaagaatgc tgttcgcttc
    12181 atgatcatca ctggtgttcg tccgattaat gtccataacc tgcgctggga ctacgtttac
    12241 gaggaggctg gtgaaattgt ttatccggaa ggggttatcg gcatgcgagg ggctatgaaa
    12301 acacaaaagg ctttccgcct gccgataacg cctgagatcc ggcggattat cgacgagcag
    12361 aaagcctggc gtgattcagt tcctgagtgc aacagggatt atgtattttt gcagccacgt
    12421 gatccaatgc agccattttc aaaacgatca ctggataagc tggtgaaaac atacagcccg
    12481 gacggggctg taaaaggaat aaaacatgat gggactgtta aagggaaaga cggtgcattt
    12541 aatacgatgt gccgtaaatt ccttaagagc aatgttattg ccttgatcga taggaattaa
    12601 aaccccaaaa agattaaaaa aacaccacaa aacggatgtt tcttcaacac cacttttgct
    12661 catatgaacg gaaccgacga ttaaactgga tggctctgat tgattcaggg tatgaatggc
    12721 ggttttttgc tccgtttccc tcaaaatgga cgcaactttc cctctgcggc tctcagccgc
    12781 accaccgcat ccgggccagc ggctcatgca tcaggacctg ctctgccaga cggtagcccc
    12841 gcttcagccc cgtaaaacgc atctgactcc cgcacagcac gcacttcagc gggtcaacct
    12901 tcagtaacct ctgatacatc cctctccagg tgatttgcat cgccgttttt ctcactgtct
    12961 ccgttatgat gtacaccact tcttccagta accgccgttt cgccggactc aaaaaaccgt
    13021 agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt tccatgaact
    13081 catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg taacgcagac
    13141 cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt ggctttttca
    13201 ggtaagagcc aaagtacacc gccacatggg gggcattatc catcacccgg gatacgttga
    13261 cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt ttactgcttc
    13321 cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac agcaaccgtg
    13381 ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga tttttccagg
    13441 tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga tgccactgct
    13501 ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct gcctgatggc
    13561 agatttccag tatcacatcc gctgcaatgc ggctcatctc tgtcagtaac caccggttgt
    13621 ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc cacgggcagt
    13681 cggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc ccgcagtgcg
    13741 ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag tccggtgatg
    13801 aacagcacca ctgtgtatac cccatcagtg tggtcccgca cgccatgatt ttggtcaccg
    13861 actcaatcac caccggacgt actgcccttt ccggctgctt ctccagccag ttaagccagt
    13921 ggtttccctg ctgaaagata tcggcaaaac ggggaagcat cagaagggcg gggcgactcc
    13981 gtccggccag tgaaccgtgc cacactccgg gcagtacata ccgccggcgc tgataccgga
    14041 aagaatggtc gcaaattccc gctccgtgca gcgggcgatt tccggatacc cttcgtcatc
    14101 aacacgtaca aaccagaaga ccagcttttt gtttcccgca tccacaaaga acggaatatt
    14161 caggtctgcg cagcattcaa cggcatcgtc aaaactatca aagcgcagaa cttctgcgtc
    14221 ttcttcgtca aaaaaatcat cttcgtgaag cttcacgaca tagcggggaa gtttgcttct
    14281 ttgagaggcg ggtttac
//
LOCUS       DAJTVE010000105        14160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000105 DAJTVE010000000
VERSION     DAJTVE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..229)
                     /locus_tag="OO439_003033"
     CDS             complement(<1..229)
                     /locus_tag="OO439_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6317340.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            386..562
                     /locus_tag="OO439_003034"
     CDS             386..562
                     /locus_tag="OO439_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6317341.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1188..1487
                     /locus_tag="OO439_003035"
     CDS             1188..1487
                     /locus_tag="OO439_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317342.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1534..2421)
                     /gene="rarD"
                     /locus_tag="OO439_003036"
     CDS             complement(1534..2421)
                     /gene="rarD"
                     /locus_tag="OO439_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6317343.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2473..2940)
                     /locus_tag="OO439_003037"
     CDS             complement(2473..2940)
                     /locus_tag="OO439_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6317344.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3105..3974
                     /gene="pldA"
                     /locus_tag="OO439_003038"
     CDS             3105..3974
                     /gene="pldA"
                     /locus_tag="OO439_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6317345.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4107..5936
                     /gene="recQ"
                     /locus_tag="OO439_003039"
     CDS             4107..5936
                     /gene="recQ"
                     /locus_tag="OO439_003039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6317346.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            6000..6617
                     /gene="rhtC"
                     /locus_tag="OO439_003040"
     CDS             6000..6617
                     /gene="rhtC"
                     /locus_tag="OO439_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6317347.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6679..7299)
                     /gene="rhtB"
                     /locus_tag="OO439_003041"
     CDS             complement(6679..7299)
                     /gene="rhtB"
                     /locus_tag="OO439_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6317348.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7410..8432
                     /gene="pldB"
                     /locus_tag="OO439_003042"
     CDS             7410..8432
                     /gene="pldB"
                     /locus_tag="OO439_003042"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6317349.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8440..9240
                     /gene="yigL"
                     /locus_tag="OO439_003043"
     CDS             8440..9240
                     /gene="yigL"
                     /locus_tag="OO439_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6317350.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9316..10215
                     /gene="bioP"
                     /locus_tag="OO439_003044"
     CDS             9316..10215
                     /gene="bioP"
                     /locus_tag="OO439_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6317351.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10103..11056)
                     /gene="metR"
                     /locus_tag="OO439_003045"
     CDS             complement(10103..11056)
                     /gene="metR"
                     /locus_tag="OO439_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6317352.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11292..13553
                     /gene="metE"
                     /locus_tag="OO439_003046"
     CDS             11292..13553
                     /gene="metE"
                     /locus_tag="OO439_003046"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6317353.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13838..14083)
                     /locus_tag="OO439_003047"
     CDS             complement(13838..14083)
                     /locus_tag="OO439_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6317354.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3448 a         3425 c         3689 g         3598 t
ORIGIN      
        1 aacttgaggc actaaaagca acattaatag aacctgggcc agggaaatgg tatttaataa
       61 gtctgtaagg atcaactttt gctgagatat cgcttaggcc gagtgagaga aagtcttgtt
      121 ctaccatatc accatattgc atatcatcag cagaaaaatc atcgaataaa ctaatagttt
      181 cgaaaagtgt acagggaata ttaattggcg gaagtaatat gttattcatt tgcatttgcc
      241 ttggttatat ttaagctcaa gatcaattcg agaacaaatg ctagggtaaa aaaatattta
      301 aaaaagaagt acacttcaca ttttttcatt acatctctga agagaacctc gttaatgatt
      361 ggtcttgctc attgttaata acaaaatgtt gaatttaccg attaggaaac tttttcctac
      421 gcggattgcc tccagctatg gcatgaactt tgagtttatg ccagaactga agtggagctt
      481 tggctaccct ggcgcgatta tctttatgat ccttgcgggc ctgacaccgt atctgtactt
      541 taagcgtaag aactggctgt aactatacga tgtcgctgtg agtaagttag tgtgctgaca
      601 gcgacatttt agccgaggga atcgcagata acataattgt tgtgaataat atccatgcca
      661 cgttgagttg gacgcttaat gactcacatc tcaccccttc aattgctgca gagataatag
      721 aaaaaagtgt caaacccaat gaaatcaata caaagtagat tgccttacat ccagtatctc
      781 ttattctttt tgctccaata ttgatattta cataggtatt attaataacc agaataaaac
      841 tcaaagccag gttgatatca ttcaaagtta ttaatattga ccatgatgtt aatgtataga
      901 ttatgaactg aaagcgtgat aaacgctcaa gcctggatgg gtagaatata tttctgatgg
      961 atgatttgaa catttttaca ttgttttata tatataattg ttttgaatat atattacctg
     1021 aattatgtgg tttgcatata tatatgcaat atagcagtct ttgcattgat aactatacag
     1081 tagtaattta gagtgtgtaa gtttttattg atggggtata ttttaattaa atataattga
     1141 aatgtatttt tattctataa acaatatctt gggtgaatgg attctatatg ggaaatggta
     1201 acttttggca gttgttcttg ggtaaagaac cttacagcaa aaaatctgat tttgttaagg
     1261 catggttatt gtggtgggct attaccatcc ttgttctatt tattttaagc ctgactacga
     1321 tattaaattt aagaagtata ggtatagggt taagttttgg gttttgtcaa atccttggta
     1381 gatatacgat gtctctagga tggagtaagt actggggagt tttgggggta tttcctgtaa
     1441 caaatattgt tctttttctt atcttaattt ttttcaaaaa aagataaata agtcagtggg
     1501 taggctcttt aaacggccta cccatgttaa ttcttaactt tttctacgct gcgtataaat
     1561 cgcatccatc acaaaaattg ccagcgccac ccaaataaag gcgaaagtca ccatcttatc
     1621 ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg tcgggccaat
     1681 gtactggaaa aagcctaacg ttgagagacg caagcgcgtg gcagcggcgg taaaacacaa
     1741 cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg acatcgggtt
     1801 ttgccccata tggctggttg agctgtcggc aatagcaaac aggtaaattg ccgccacggg
     1861 cagcagccac atggtttcga ttaacatgcc ggtttgcgct tcaacggcaa tcttcttgcg
     1921 taccagaccg tagaaggcaa aactaaatgc cagtcccagc gcgataatag gtagcgaacc
     1981 aaaagtccac agctggacta acacgccaca tatcgccaga atcaccgcca gccattgcat
     2041 ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca gcgggttaat
     2101 aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga taaacagtag
     2161 ccagttgcca ccaatcagca cggcagagac tgccagcata aaaattttct gtggcgtctg
     2221 aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca ccataaagaa
     2281 aaacgaccag atcacgcgat gcgtcaggat ttcatcggcg ggcacgtagt aaatcaactt
     2341 gaagtacgct ggcgctatac cccaaataaa ataagcggca agagcgagta atacgccctg
     2401 ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag cagtttacct
     2461 gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat aaagctgttc
     2521 ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca acaggctact
     2581 ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa gatcaatggt
     2641 ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt ggcgggttaa
     2701 ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa tgaccccgcc
     2761 gtgcaaaatg ctttgcgccc agttgcccac catcattggc tgatttttaa aggccagctg
     2821 tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa atggcatgtg
     2881 ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta cggcagacat
     2941 atcatcctta cacttcattg gttaatgaaa tgttgatttc atgcttcttt gttgttggtt
     3001 tctactttag gaagggataa ctaacggcta tggagttaag tatgtaaaat agcccgcaga
     3061 aaaatattca ccttatcaat aattcgttac ggagaacacg accgatgcgg actctgcagg
     3121 gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag gcaacggtga
     3181 aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg ctgcaggagc
     3241 atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatttac acccaaacca
     3301 gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg cgtaaggatg
     3361 aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta ggcccgaact
     3421 cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat agcgaagagt
     3481 cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt gccaccgatt
     3541 actgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac gactctaacg
     3601 ggcgttccga cccgacctcc cgcagctgga accgccttta tactcgcctg atggcagaaa
     3661 acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact gacgataacc
     3721 cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac ctcggtgatg
     3781 cggtgttgag tgcgaaagga cagtacaact ggaataccgg ttacggcggc gcggagttag
     3841 gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac agcggctatg
     3901 gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtagga gttatgctaa
     3961 acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaacccagc atccaggatt
     4021 accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa tagcgcctgt
     4081 ttttatttca ggcaatcggg gggaatgtgg cgcaggcgga agtgttgaat ctggagtccg
     4141 gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc ggccaggaag
     4201 aaattatcga cactgtgctt tccggtcgcg attgcctggt cgtcatgccc accggtggcg
     4261 gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc gtggttgttt
     4321 caccgctgat ttcgttgatg aaagatcagg tggatcaact gcaagccaac ggcgtggcgg
     4381 cggcgtgcct taactcgacg caaacccgcg agcagcaact tgaagtgatg acaggctgcc
     4441 gcaccgggca aattcgtctg ctttatatcg ccccggaacg cctgatgctg gataactttc
     4501 ttgagcatct ggcgcactgg aatccggtgt tattagccgt tgatgaagcg cactgtatct
     4561 cccaatgggg ccacgatttc cgcccggaat atgccgcgct tggtcagtta cgccagcgtt
     4621 tcccgaccct gccgtttatg gcgctgactg ccacagccga cgacaccacg cgccaggata
     4681 tcgtgcgctt gctggggctg aacgatccgc tgattcaaat cagcagtttt gaccgtccga
     4741 atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg cgctacgtgc
     4801 aggaacagcg cggtaagtcc ggcattatct actgcaacag ccgggcgaaa gtagaagaca
     4861 ccgctgcgcg cctgcaaagc aagggtatta gcgcggcggc ctatcatgcc gggctggaaa
     4921 ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgacctgcaa attgtggtgg
     4981 cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg gtccactttg
     5041 atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg cgtgatggcc
     5101 tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg cgccgttgtc
     5161 tggaagagaa gccgcagggg cagttgcagg atattgagcg ccacaaactc aatgcgatgg
     5221 gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat tttggcgaag
     5281 ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa cagtacgacg
     5341 gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag cggtttggga
     5401 tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc gactatggtc
     5461 atgacaaact gaaagtctat ggcatgggcc gtgataaaag ccatgaacat tgggtgagcg
     5521 tgatccgcca gctgattcac ctcggcctgg tgacgcaaaa tattgcccag cattctgccc
     5581 tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg caacttgccg
     5641 tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc ggcaactatg
     5701 atcgtaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat gaaagtaatg
     5761 tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa cagatgccga
     5821 tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg gaacgctttg
     5881 gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa gagtagtcag
     5941 cagcataaaa aagtgccagg atgaagactc cgtaaacgtt tcccccgcga gtcaaatgta
     6001 tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg agccccggtc
     6061 ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa gcgatgatgg
     6121 gcgtgctggg cattacctgc ggtgtaatgg tttgggctgg gattgcgttg cttggcctgc
     6181 atttgattat cgaaaaaatg gcctggctgc atacgcttat ggttggcggt ggcctgtatc
     6241 tctgctggat gggttaccag atgctgcgcg gcgcgctgaa aaaagaggcg gtttctgcac
     6301 ctgcgccaca ggtcgagctg gcgaaaagtg gacgcagttt cctgaaaggt ttactgacca
     6361 atctcgctaa tccgaaagcg attatctact ttggctcggt gttctcattg tttgtcggtg
     6421 ataacgttgg cactaccgcg cgctggggca tttttgcgct gatcattgtc gaaacgctgg
     6481 cgtggtttat cgtcgttgcc agcctgtttg ccctgccgca aatgcgccgt ggttatcaac
     6541 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
     6601 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
     6661 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
     6721 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
     6781 agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
     6841 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
     6901 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
     6961 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaaggtcaa ttgcaccagc
     7021 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc acttcaacac
     7081 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
     7141 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     7201 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     7261 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     7321 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctaa aaaaaacgat
     7381 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     7441 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     7501 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     7561 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     7621 cggaactggc ctatgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     7681 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     7741 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     7801 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     7861 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     7921 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     7981 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     8041 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     8101 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     8161 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttgcaggct gaagaggaac
     8221 gcgtggtgga taaccgcatg catgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     8281 ctgtcgaagg aggacgaccg ttggtaatta aaggtgctta ccatgagatc ctttttgaaa
     8341 aggacgcaat gcgctcagtc gcgctccacg ccattgttga ttttttcaac aggcataact
     8401 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     8461 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaacc
     8521 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     8581 gatgtggggc aaattcgcga taatctggag atcaagtcgt acatgatcac ctccaacggc
     8641 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     8701 gccagcgatc tgtttggcgt agtcaacgac aatctggaca tcattactaa cgtttatcgc
     8761 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     8821 gtgttcaaat atgcgctgta tgagcctgga ttactggagc cggaaggcgt cagcaaagtg
     8881 ttcttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgt
     8941 tggggcgatc gcgtcaacgt tagtttctct accttaacct gtctggaagt gatggcgggc
     9001 ggcgtttcaa aaggccatgc gctggaagcg gtggcgaaga aactgggcta cagcctgaag
     9061 gattgtattg cgtttggtga cgggatgaac gatgccgaaa tgctgtcgat ggcggggaaa
     9121 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagacc ttcatcccga gctggaagtg
     9181 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     9241 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     9301 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     9361 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     9421 gttggcctgg cggcactcgt ttttctgccg tttctgcgta cccgtggcaa tagcctgaaa
     9481 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     9541 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     9601 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     9661 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggttaccgac
     9721 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     9781 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     9841 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     9901 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     9961 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
    10021 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
    10081 cagccgcact ggccaacgtt cattacaggc gcgctggtga tcctggcctc actgtgggtg
    10141 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
    10201 gcgctgagcg aataaatgct tctgtaatcg gctgacgctg ctcgccatcg cgcacggcgg
    10261 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccttggcgct
    10321 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccatct
    10381 gaatcaacaa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
    10441 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaatcaac agcgtctcgc
    10501 tggcgagatc ttccggtgta attcgcgttt tcgccgccag tggatggtca ggtgctaaca
    10561 ccagacgcac ttcatagtcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
    10621 cggaggtcat caccagatcc agctcgccct gttgcaacgc aggctgcggg tcaaacgtca
    10681 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaacgcgg
    10741 gcgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
    10801 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
    10861 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
    10921 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
    10981 acgtcgccgc agcggctgcg agagagccgc agttccgcaa cgcttgtagc gttttcaggt
    11041 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
    11101 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
    11161 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
    11221 gggatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
    11281 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
    11341 gcgagctgaa aaaagcgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
    11401 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
    11461 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
    11521 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
    11581 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
    11641 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
    11701 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg ggccacaacg
    11761 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
    11821 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
    11881 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
    11941 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
    12001 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
    12061 tgcctgttca gggtctgcat gttgatctcg tacatggtaa agatgacgtt gctgaactgc
    12121 acaagcgcct gccttctgac tggttgctgt ctgcgggtct gatcaatggt cgtaacgtct
    12181 ggcgcgccga tcttaccgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
    12241 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
    12301 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactag
    12361 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
    12421 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
    12481 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
    12541 agcgtgcacg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
    12601 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaataact
    12661 accgcacggg cattgcggaa catatcaggc aggccattgt tgaacaggaa cgtttgggac
    12721 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
    12781 acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
    12841 tgaagccacc gattgttatt ggtgacgtta gtcgcccggc accgattacc gtggagtggg
    12901 cgaagtatgc gcaatctctg actgataaac cggtgaaagg gatgctgact ggcccggtga
    12961 ctattctctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
    13021 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
    13081 agattgacga accggctctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
    13141 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
    13201 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcac
    13261 tggacgcaga cgtcatcacc atcgaaacct cgcgttctga catggagttg ctggagtcgt
    13321 tcgaagagtt tgattatccg aatgaaatcg gtccaggcgt ctatgacatt cactcgccaa
    13381 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
    13441 cagagcgcct gtgggttaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
    13501 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcgggga tgatgaatat
    13561 taacgagtca ctgtaaattg cagtacgatg aaagaggcaa aactgcctga tactaggcct
    13621 acataattta tgcaatttat tgaatttttg ggttttgttg gccggacaag gcgttcacgc
    13681 cgcatccggc atgaacaacg cgcactttgc caataatctg atggcttcca tcaccggaag
    13741 ccattttgct atctgcgccc gttcagatac agaaataacc gaatcatttt tacgcaaaat
    13801 ataatcctga gcgatttgtt cagtaatcaa cgctacgtta tttattctca cttttcacga
    13861 aattgtatcc cttttcagta acgtggtagt gaggcataga cttatgtccg ctatcctcaa
    13921 taataaccaa accctcgtta gctaatttgt taacaatgtt aacaacatta ccgaatcctg
    13981 gaatttgcct tatggatagt gtgcgatcga gattcatcca gttccagttt atatctttct
    14041 cagccataat tttcagtacg ttaatttcag tttcagttat catagtcaag tgcatcctgg
    14101 tggtataagt cattaagttc tcccttaagt ttataatctt ctagagtagc agtatgcgta
//
LOCUS       DAJTVE010000106        14098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000106 DAJTVE010000000
VERSION     DAJTVE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(3..1361)
                     /gene="hprS"
                     /locus_tag="OO439_003048"
     CDS             complement(3..1361)
                     /gene="hprS"
                     /locus_tag="OO439_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6317355.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1361..2032)
                     /gene="hprR"
                     /locus_tag="OO439_003049"
     CDS             complement(1361..2032)
                     /gene="hprR"
                     /locus_tag="OO439_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6317356.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2165..2578
                     /gene="hiuH"
                     /locus_tag="OO439_003050"
     CDS             2165..2578
                     /gene="hiuH"
                     /locus_tag="OO439_003050"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6317357.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2687..3691
                     /gene="msrP"
                     /locus_tag="OO439_003051"
     CDS             2687..3691
                     /gene="msrP"
                     /locus_tag="OO439_003051"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6317358.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3692..4327
                     /gene="msrQ"
                     /locus_tag="OO439_003052"
     CDS             3692..4327
                     /gene="msrQ"
                     /locus_tag="OO439_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6317359.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4584..5234
                     /gene="zinT"
                     /locus_tag="OO439_003053"
     CDS             4584..5234
                     /gene="zinT"
                     /locus_tag="OO439_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6317360.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5577..6107
                     /locus_tag="OO439_003054"
                     /pseudo
     CDS             5577..6107
                     /locus_tag="OO439_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6677..6766)
                     /locus_tag="OO439_003055"
     tRNA            complement(6677..6766)
                     /locus_tag="OO439_003055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6730..6732),aa:Ser,seq:cga)
     gene            6860..7657
                     /gene="mtfA"
                     /locus_tag="OO439_003056"
     CDS             6860..7657
                     /gene="mtfA"
                     /locus_tag="OO439_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6317361.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7758..7833
                     /locus_tag="OO439_003057"
     tRNA            7758..7833
                     /locus_tag="OO439_003057"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7791..7793,aa:Asn,seq:gtt)
     gene            8147..14098
                     /gene="yeeJ"
                     /locus_tag="OO439_003058"
                     /pseudo
     CDS             8147..14098
                     /gene="yeeJ"
                     /locus_tag="OO439_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3988 a         3350 c         3169 g         3591 t
ORIGIN      
        1 tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag atagtgatac
       61 gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc aagtccctga
      121 cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt ttcaggctca
      181 tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt atcaagaaaa
      241 ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac aataagattc
      301 gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca ctcgacctta
      361 aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt ttcgacttcc
      421 ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc ccgggcaaga
      481 aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt accggcaatt
      541 gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt ctgacccagt
      601 aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg acttagacgc
      661 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      721 tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt cagcgcttcc
      781 gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag cggacttaat
      841 actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt atactgttca
      901 agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      961 tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct tctgtaaatg
     1021 ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
     1081 gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc ctgactaaca
     1141 tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc aattaacaac
     1201 tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
     1261 gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag cagcaatata
     1321 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
     1381 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1441 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1501 cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg gaggccagta
     1561 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc ctgctcacac
     1621 tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc aaagcgtgat
     1681 gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa ggttttacca
     1741 gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa tcccttgcag
     1801 taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc tgccagccat
     1861 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1921 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1981 gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc atgaaaataa
     2041 tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg taatgctgct
     2101 gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg acaaggatat
     2161 aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt cattaccttc
     2221 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaaccgg
     2281 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg gctggttaca
     2341 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     2401 tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2461 tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag tgaacgagca
     2521 ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg gcagttaatg
     2581 cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct gacagattaa
     2641 cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga aaaagaatca
     2701 atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc gtcgacaggt
     2761 gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg cgcatgccga
     2821 tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa aaccgctgga
     2881 gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag ataaagtctc
     2941 cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg ctaatgctgg
     3001 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     3061 cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     3121 ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc acaaattgct
     3181 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa tttatgcacc
     3241 ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     3301 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg tgggtgttta
     3361 tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc cgtggaaata
     3421 tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc gtccgccaac
     3481 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3541 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcatcctcga
     3601 tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3661 tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac aggtgacatg
     3721 gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3781 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3841 tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct acgcaaaaca
     3901 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3961 gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt tattaggcaa
     4021 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     4081 tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact ggcaacagtt
     4141 gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     4201 gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt tagccttacg
     4261 gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca acaaattatc
     4321 tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg gcgcgagcgc
     4381 cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa cctcaaatta
     4441 tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa actatgctct
     4501 catctcctaa gagttgttgc attttgccat atgttacaat ataacattac acatcatata
     4561 caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct gttgctttag
     4621 gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac ggcaaaccct
     4681 taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat gtacaaaacc
     4741 gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg caaagtggca
     4801 aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca tttgctgaaa
     4861 ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc attgaggacg
     4921 gcattgttga attccataga aataatgaaa caacatcctg taaatatgat tacgatggat
     4981 acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag
     5041 atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa
     5101 aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta ttaaatgaaa
     5161 tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg gtcgaggaaa
     5221 tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta agtcatcata
     5281 actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga cacgctcatc
     5341 actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa taacggattc
     5401 catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct tatattggct
     5461 ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac atctgtataa
     5521 ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct acaaaaatga
     5581 accgattctc aaaaactcaa atttatttac attggataac gctgcttttc gttgcaataa
     5641 cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact tatctgctga
     5701 tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt tcacgcctga
     5761 ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc tggcaaatga
     5821 aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt cctctgctgg
     5881 ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc aatgtgtctc
     5941 cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac gaaacctagg
     6001 caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt catcactaca
     6061 ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc gatcccctga
     6121 caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag tgcacaatgc
     6181 atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa tggtaagact
     6241 tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag tgtgatacaa
     6301 aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg
     6361 gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt ctctacgcaa
     6421 cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta aaagaataat
     6481 ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc ttcctgtcag
     6541 atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa
     6601 cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga agtcaggaga
     6661 taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga gttgccccta
     6721 ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc agatggttgc
     6781 catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt gttcaagtga
     6841 cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat cagcacatca
     6901 aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc tgacggaaca
     6961 ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc ggcttgttcc
     7021 tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc tattttgcct
     7081 acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct taatttatcc
     7141 tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc ataaccaacg
     7201 tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact ggttggatat
     7261 acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg ctcataagct
     7321 ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc gtgaggttgc
     7381 tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa tcgaattggt
     7441 tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg aatgttttgc
     7501 cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt tcccttcatt
     7561 gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc atcacgctaa
     7621 tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc agattaatta
     7681 accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct aagtaatatg
     7741 tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     7801 atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct tttttagcgc
     7861 aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt taacacctta
     7921 agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac cattttcccc
     7981 tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca cccaaaactg
     8041 cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat tttttcgctg
     8101 tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg ctacgaagaa
     8161 gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa ttaaactacg
     8221 ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg ctgcggcagc
     8281 acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg ccattgcaaa
     8341 tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg ttgccgaacg
     8401 tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt ttgctcgagg
     8461 ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta gtgaaaaaaa
     8521 attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag ccagtacttc
     8581 acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg caaatatggc
     8641 gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa gccgcttcgg
     8701 taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact cccagttcga
     8761 ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc atactctcca
     8821 tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt tcactcccac
     8881 atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc actcccgcgt
     8941 cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg gctatttgcg
     9001 actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac gcccggccaa
     9061 tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg gcggtaaact
     9121 ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg atcggcaaag
     9181 taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga tgaccttcag
     9241 cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg tcgattttac
     9301 ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg ctgcacggcg
     9361 tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg ttctggaata
     9421 tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga agtcaggaga
     9481 agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct ataacgtcga
     9541 agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag atattctagt
     9601 taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct ggccgattga
     9661 agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca tggtggtcgt
     9721 tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc aaacattgaa
     9781 cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag caggtaatcc
     9841 tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca ccctttctga
     9901 ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag cgatgtctgg
     9961 cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc ccgccgtggt
    10021 gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg ataaggaccg
    10081 ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa atgacaaacc
    10141 tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg tgaaaccagg
    10201 agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct ataccgccta
    10261 taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat accgctggat
    10321 ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc agcaataatg
    10381 gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct gatgaaggaa
    10441 gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg gcaacttcct
    10501 tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt gatctgaaaa
    10561 gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg aaacaaacgt
    10621 taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag aagtcgaaaa
    10681 atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc cgggatgcaa
    10741 aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca gaggcgaaac
    10801 tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg accagtttga
    10861 aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct aatcaacagg
    10921 tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct tcaggtgata
    10981 tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat aaaaatggca
    11041 acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca agtaagttct
    11101 cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc gcctccctga
    11161 ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc aatgtcagcg
    11221 atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt ttgcaaacat
    11281 cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca acagtgaaag
    11341 atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca caggacgttg
    11401 cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat ggggaagcgc
    11461 atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg ctgggtaata
    11521 acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg gctcaggttg
    11581 tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca acgctaactg
    11641 caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca ttcagctcag
    11701 cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct ggcatcgcgc
    11761 aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca ctggctaata
    11821 atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg gcaaaaatta
    11881 tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac agctccggca
    11941 gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt gtgactgtga
    12001 acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc gtaacgaacg
    12061 aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa tcaggagcga
    12121 gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc acatcaatta
    12181 atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt tttgatacag
    12241 tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac ggcaacggtg
    12301 tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc cccagtaata
    12361 acgctatata tactaccaac cacgacggca atttttacgc aagctttacc gctacaaaag
    12421 ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga
    12481 cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag gatccggtga
    12541 ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca gagggcaatg
    12601 cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg aacttcacgc
    12661 tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc acgctgaaag
    12721 gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag agtgagcagt
    12781 tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac gttaccgagg
    12841 acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg actgatggaa
    12901 acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat gtgagcgcaa
    12961 gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag gctgaagtta
    13021 cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac aattatggtg
    13081 tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca ctagcctcct
    13141 taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg actgcaagcg
    13201 tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc ggaacctccg
    13261 tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa attcttgtga
    13321 caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa cctactgaag
    13381 tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt accagtctgg
    13441 agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct cacgtcaacg
    13501 accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag ccaccagagc
    13561 acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc gaggtctcca
    13621 tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat ggagcctcac
    13681 ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc agcccgctta
    13741 tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct gcaaatggca
    13801 ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg acgttaagtg
    13861 gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc agcaataaag
    13921 tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag acacagacaa
    13981 ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc gctgatccat
    14041 cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt gaggatgg
//
LOCUS       DAJTVE010000107        13945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000107 DAJTVE010000000
VERSION     DAJTVE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..911
                     /gene="rtcB"
                     /locus_tag="OO439_003059"
     CDS             <1..911
                     /gene="rtcB"
                     /locus_tag="OO439_003059"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6317362.1"
                     /translation="VPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPR
                     FLNTNNYKHLGTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQ
                     DQLETLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTV
                     RQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVR
                     GLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKD
                     IDAVMAAQSDLVEVIYTLRQVVCVKG"
     gene            915..1931
                     /gene="rtcA"
                     /locus_tag="OO439_003060"
     CDS             915..1931
                     /gene="rtcA"
                     /locus_tag="OO439_003060"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6317363.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(1974..4679)
                     /gene="malT"
                     /locus_tag="OO439_003061"
     CDS             complement(1974..4679)
                     /gene="malT"
                     /locus_tag="OO439_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6317364.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5290..7683
                     /gene="malP"
                     /locus_tag="OO439_003062"
     CDS             5290..7683
                     /gene="malP"
                     /locus_tag="OO439_003062"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6317365.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            7693..9777
                     /gene="malQ"
                     /locus_tag="OO439_003063"
     CDS             7693..9777
                     /gene="malQ"
                     /locus_tag="OO439_003063"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6317366.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(9822..11138)
                     /gene="gntT"
                     /locus_tag="OO439_003064"
     CDS             complement(9822..11138)
                     /gene="gntT"
                     /locus_tag="OO439_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6317367.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11499..12074)
                     /gene="nfuA"
                     /locus_tag="OO439_003065"
     CDS             complement(11499..12074)
                     /gene="nfuA"
                     /locus_tag="OO439_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6317368.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12133..12816)
                     /gene="gntX"
                     /locus_tag="OO439_003066"
     CDS             complement(12133..12816)
                     /gene="gntX"
                     /locus_tag="OO439_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6317369.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            12854..13624
                     /gene="bioH"
                     /locus_tag="OO439_003067"
     CDS             12854..13624
                     /gene="bioH"
                     /locus_tag="OO439_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6317370.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13653..>13945)
                     /locus_tag="OO439_003068"
     CDS             complement(13653..>13945)
                     /locus_tag="OO439_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6317371.1"
                     /translation="CLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDGVAERSPKHK
                     ESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAAASQ"
BASE COUNT         3343 a         3483 c         3894 g         3225 t
ORIGIN      
        1 ccgtaccaca cgggcgtact accgggcgtt gtaaacgtga taaaggcgca tgggaaaacc
       61 cgccttttaa cgtcgatgcg aaatgggctg agctggaggc cggttatcag tggttaacgc
      121 aaaaatatcc gcgtttcctt aacactaaca actataaaca cctgggaacg ctgggaacgg
      181 gtaaccactt tattgaaatc tgccttgatg agtcggagca ggtgtggatt atgctgcact
      241 ccggttcacg cggaattggt aacgccatcg ggacttactt tatcgatctg gcgcaaaaag
      301 agatgcagga ccaacttgag acgttgccgt cgcgtgatct ggcgtacttt atggaaggta
      361 cggaatactt tgatgattac ctaagagccg tggcctgggc gcagcttttc gccagcctta
      421 accgcgatgc gatgatggaa aacgtggtaa cggcattgca gagcattacg cagaaaacgg
      481 tcagacagcc acaaacgctg gcgatggaag agatcaactg tcaccacaac tatgtgcaaa
      541 aagaacagca ctttggtgaa gagatctacg tgacgcgtaa aggcgcggtg tttgcgcgtg
      601 ctggtcaata tggaattatt ccagggtcga tgggagcaaa aagctttatc gtccgtgggc
      661 tgggaaatga agaatctttc tgttcgtgca gccacggtgc cgggcgggta atgagccgta
      721 ctaaagcgaa aaaactgttc agcgtggaag atcaaattcg tgccaccgcg catgtggaat
      781 gccgtaaaga tgccgaagtg atcgacgaaa tcccgatggc gtataaagat attgatgcgg
      841 tgatggcggc acaaagcgat ctggtggaag ttatctatac cctgcgtcag gtggtgtgcg
      901 taaaaggata aatgatgaaa aggatgattg cgctggatgg cgcacagggc gaaggtggcg
      961 ggcagatcct gcgctcggcg ctgagcctgt cgatgataac cggccagcca tttaccatca
     1021 ccagcattcg tgccgggcgg gcgaaaccgg ggctgttgcg ccagcatctg accgcggtaa
     1081 aagcggctgc ggaaatttgt agggcaacgg tggaaggcgc ggagctgggg tcgcagcgtc
     1141 tggtcttccg gcttggcacc gtgcgcggcg gcgagtaccg ctttgctatc ggtagtgccg
     1201 gaagctgtac gctggtgctg caaacggtgc tgcccgcgct gtggtttgcc gatggacctt
     1261 cgcgtgttga agtgagcgga ggcaccgata acccgtcggc cccgcctgcg gattttatcc
     1321 gccgggtgct ggagccgctg ctggcgaaaa taggaattca tcagcaaacc acgctgttac
     1381 gtcacggttt ttatcctgcc ggaggcggcg tggtggcaac ggaagtctcg cccgtggcat
     1441 cgtttaacac cttgcaactt ggcgagcgcg ggaacattgt gcagatgcgt ggagaagttc
     1501 tattagctgg tgtgccgcgc catgttgctg agcgtgaaat cgctacactg gcggggagtt
     1561 tttccctgca tgaacagaat attcataacc tgccgcgcga ccaggggccg ggtaataccg
     1621 tctcgctgga agtcgaaagt gaaaatatca ctgaacgctt tttcgtcgtc ggtgaaaagc
     1681 gcgtcagtgc cgaggtggta gccgcgcagt tagtgaaaga ggtgaaacgc tacctggcaa
     1741 gcccggcggt agtgggggaa tatctcgccg accagctggt gctaccgatg gcgctggcgg
     1801 gcgcgggaga atttacggtc gcccatcctt catgccatct gctgaccaat atcgccgtgg
     1861 tggagcgttt cttgccggtg cggtttggtt tgatagaaac agatggcgta acgcgggtga
     1921 gtattgaatg atgtaaagat gtaaaccgga tccaatgcat tatccggcta aactcacacc
     1981 ccatatccca tcatcttcag cagtttctgc gcgtgttgta ccgcggcctg acggtgggcg
     2041 acgccgagtt tttggtacag attgcggata tgcgtcttga tggtggttgc cgccacttcc
     2101 agctcgcccg caatttgctc attgctgtaa ccggagtaga tcagccccag gacctgccat
     2161 tcacgttggg tcaacgggct ggtgtggatc agttctggca cttccggatg attcagcaga
     2221 cgttcaacga aattctcatc gaaatgggcg aatttatgcc gatgatgttg attgatttct
     2281 cgcagaatac gctgcgcgcg atgctgttcc agttccggca gcgtattgag ttgaatcagc
     2341 tggcgcagtt gttgcgccat cgcttcgcct tcgatgacaa aatgactgat aaatccggtg
     2401 cgattcgcca gttttaatgc gtccagcaac acgcgctggg cgtcactttt acgtccggcc
     2461 tgccagtaca gttgattaag cagcaacagg ttacggttga gatcgctcat caaccgcaga
     2521 ctgcgggcat tttcattgag ttcttcgaga acaatttccg caggttcaaa ctcgcccagc
     2581 aagatttgtg cgcgggcaat gttgcgccat tgaccttgca ggaagtggtt gttcgcaaac
     2641 tccggtttag ccgtatgacg caaccagttg gcagcggcgg ctttatcgcc ggtcatttgc
     2701 cagtaaatca cccggacttt gttggcgtta gagatccagt cgctgtgata tttgccattc
     2761 cccagcaggt tttccagacg gttcagctgg ctacgggcgt tatctaaatc accacgggcc
     2821 agcgagcatt gaatcaacat tgccaggcac tgaagctgtt gctgtggctg ataagacgac
     2881 aagacttcaa tcccgctacg cgccgacgct tcggcttcat ccagccgtgc ccaggcccat
     2941 aacagttgtg ctcggatgcg caccagaaac tcatgcatcg gcagctgttc cagatgctgc
     3001 tcgttgatca gctggaacgt tttttcctgt gtttcccacg cggtttgcag gaacccttgg
     3061 gcaaacagaa tttcactttg ctggattaaa ctccataagg cgtagtgcca gacgtcgtgc
     3121 tggcgtgcca tctgttcggt ttgctgcatt agcgccagtg agcgggtcaa ttcgcctttg
     3181 cagtgcagca cttcacccag caccgaggtt gctacaatgc ggctatagaa ccagcccggt
     3241 ggcagctctt ccagtgccag ttttgccagc cgttccgctt catccggatt accatcgtta
     3301 atcgccacct gggcgcgcag agcgttaaat tctgcgtgca tggtgccttc tctgatgtcc
     3361 ttgatttcat gttcagcacg ggctagcagg gtgttaactt cgccatagcg atgttggctt
     3421 tgcattagcc acgcctgcaa taacactaac tgcggatttt ccagcaaact gtcccacggc
     3481 agggccttaa gcgactcttc cagcagcgac agttcgctat ggttgaacaa actccaggcg
     3541 tgattaagca gaatatcgcg cagcatcagc gcatcgcctg ccgccagcgc atgatgaatc
     3601 gcttcgctgg gaaatccctg ggccatccag ctttctgcgg cggcacggtg gatttccggc
     3661 agctccgccg ccagttccca ctggcagcgc tggcgcagga agttgccaaa cagcgggtga
     3721 tagcagaacc actcgccggt atcatccatc cgctgtaaaa acagcccctg acgctcaatc
     3781 tcttcgagac gcatttgccc gttttcttcg ccagtcacac gggtgatgag ggcatcgttc
     3841 attgagcgca aaatggcgct tttcaacaga aaatggcgcg ttgcgagatc gacgttatcc
     3901 aaaacctcat cgaccagata atccgaaaga tggctggcat tgattcccgc caggcggcgt
     3961 gccgacttat gggctgagtg ggtattctgc cgggcggaga gggcgattag ctgtagtgcc
     4021 gtcgcccaac cggaaacgtc atcgcaaatc caactgcttt ctgcggcttc aatcggcgat
     4081 gacagacggc aatcaaaaaa ctgcttcgct tcctgatggg taaatgccag ttgctgactg
     4141 ccaatttcca gcagttggtc acgaacacgc agattggcaa tgcccagttg cggaaggttg
     4201 cgtgacaaca ccaccagggt gagattttct ggttgatggc gaataaagaa gcgcattgac
     4261 tcgtggatca ctggattagt gatcagatga tagtcatcga tgaccagata aagtgggcta
     4321 tgccattccg ccagctcaat gaaaagctgg gcgaagagtg acgtcaggct ggcatattgc
     4381 cgtttttgcg ccatcgtctc acatatcgca cagtgaccgt tggttgcctg ctgcacggcg
     4441 gcaatgagat agctggcgaa acgctcttgc tggttatcac cttcatccag cgagtaccag
     4501 ccgatatcgt ttttgcctgc cgcccactgg gaaatgaggg tggtctttcc gtagcccgca
     4561 ggactcgtga tcagcgccag ccggaagttg ttcgcgccgg aaagtttagc cagcaggcgc
     4621 tcacgaacca cggtatggtc gagtcgaacc ggacgactta gttttgacgg aatcagcata
     4681 gttaatcact tcactgtgga aaatgaggaa atattatttt tttgcgcttc gtaattaatg
     4741 gttataaggt cggccagaaa cctttctaat gcaagcgatg acgttttttt atgtgtctga
     4801 atttgcactg tgtcacaatt ccaaatcttt attaacaact cacctaaaac gacgctgatc
     4861 cagcgtgaat actggtttcc cttatgttca tcagattcat ttaagcaagg gtttcttctt
     4921 cattcctgat gaaagtgcca tctaaaaaga tgatcttaat aaatctatta agaatgagat
     4981 ggagcacatt ggatatttta cttgtgaaac tgtttcactc ctttacttaa tttatagagt
     5041 taccttccgc tttttgaaaa tacgcaacgg ccattttttg cacttagata cagattttct
     5101 gcgctgtatt gcattgattt gctgctaatc ctgtggtttg cactagcttt aagtggttga
     5161 gatcacattt ccttgctcat ccccgcaact cctccctgcc taatcccccg caggatgagg
     5221 aaggtcaaca tcgagcctgg caaactagcg ataacgttgt gttgaaaatc taagaaaagt
     5281 ggaactccta tgtcacaacc tatttttaac gataagcaat ttcaggaagc gctttcacgt
     5341 cagtggcagc gttatggctt aaattctgcg gctgaaatga ctcctcgcca gtggtggcta
     5401 gcagtgagtg aagcactggc cgaaatgctg cgtgctcagc cattcgccaa gccggtggcg
     5461 aatcagcgac atgttaacta catctcaatg gagtttttga ttggtcgcct gacgggcaac
     5521 aacctgttga atctcggctg gtatcaggat gtacaggatt cgttgaaggc ttatgacatc
     5581 aatctgacgg acctgctgga agaagagatc gacccggcgc tgggtaacgg tggtctggga
     5641 cgtctggcgg cgtgcttcct cgactcaatg gcaactgtcg gtcagtctgc gacgggttac
     5701 ggtctgaact atcaatatgg tttgttccgc cagtcttttg tcgatggcaa acaggttgaa
     5761 gcgccggatg actggcatcg cagtaactac ccgtggttcc gccacaacga agcactggat
     5821 gtgcaggtag ggattggcgg taaagtgacg aaagacggac gctgggagcc ggagtttacc
     5881 attaccggtc aagcgtggga tctccccgtt gtcggctatc gtaatggcgt ggcgcagccg
     5941 ctgcgtctgt ggcaggcgac gcacgcgcat ccgtttgatc tgactaaatt taacgacggt
     6001 gatttcctgc gtgccgaaca gcagggcatc aatgcggaaa aactgaccaa agttctctat
     6061 ccaaacgaca accatactgc cggtaaaaag ctgcgcttga tgcagcagta cttccagtgt
     6121 gcctgttcgg tagcggatat tttgcgtcgc catcatctgg cggggcgtaa actgcacgaa
     6181 ctggcggatt acgaagttat tcagctgaac gatacccacc caactatcgc gattccagaa
     6241 ctgctgcgcg tgctgatcga tgagcaccag atgagctggg atgacgcttg ggccattacc
     6301 agcaaaactt tcgcttacac caaccatacc ctgatgccag aagcgctgga acgctgggat
     6361 gtgaaactgg tgaaaggctt actgccgcgc catatgcaga ttattaacga aattaatact
     6421 cgctttaaaa cgctggtgga gaaaacctgg ccgggcgatg aaaaagtgtg ggccaaactg
     6481 gcggtggtgc acgataaaca agtgcatatg gcgaacctgt gtgtggttgg cggtttcgcg
     6541 gtgaacggtg ttgcggcgct gcactcggat ctggtggtga aagatctgtt cccggaatat
     6601 caccagctat ggccgaacaa attccataac gtcaccaacg gtattacccc acgtcgctgg
     6661 atcaaacagt gcaaccctgc actggcggct ctgttggata aatcactgca aaaagagtgg
     6721 gctaacgatc tcgatcagct gatcaatctg gaaaaattcg ctgatgatgc gaaattccgt
     6781 cagcaatatc gcgagatcaa gcaggcgaat aaagtccgtc tggcggagtt tgtgaaagtt
     6841 cgtaccggta ttgagatcaa tccacaggcg attttcgata ttcagatcaa acgtctgcat
     6901 gagtacaaac gccagcacct gaatctgctg catattctgg cgttgtacaa agaaattcgt
     6961 gaaaacccgc aggctgatcg cgtaccgcgc gtcttcctct tcggcgcgaa agcggcaccg
     7021 ggctactacc tggcgaagaa tattatcttt gcgatcaaca aagtggctga cgtgatcaac
     7081 aacgatccac tggttggcga taagttgaag gtggtgttcc tgccggatta ttgcgtttcg
     7141 gcggcggaaa aactgatccc ggcggcggat atctccgaac aaatttcgac tgcaggtaaa
     7201 gaagcttccg gtaccggcaa tatgaaactg gcgctcaatg gtgcgcttac tgtcggtacg
     7261 ctggatggtg cgaacgttga aatcgccgag aaagtcggtg aagaaaatat ctttattttt
     7321 ggtcataccg tggaacaagt gaaggcaatt ctggccaaag gctacgaccc ggtgaaatgg
     7381 cggaagaaag ataaggtgct ggacgcagta ttgaaagagc tggaaagcgg taaatacagc
     7441 gacggcgata agcatgcctt cgaccagatg ctgcacagta tcggcaaaca aggcggcgat
     7501 ccgtatctgg tgatggcgga tttcgcagcc tatgttgagg cacaaaagca ggtggatgtg
     7561 ctgtaccgcg accaggaggc ctggactcgc gcggcgatcc tcaataccgc ccgctgcggt
     7621 atgtttagct cggatcgctc tattcgcgat tatcaggctc gtatctggca ggcaaaacgc
     7681 taaggaagct cgatggaaag caaacgtctg gataatgccg cgctggcggc ggggattagc
     7741 cccaattaca tcaatgccca cggtaaaccg cagtcgatta gcgccgaaac caaacggcgt
     7801 ttgcttgacg cgatgcatca acgtaccgcc acgaaagtgg cggtaacgcc agtcccgaat
     7861 gtcatggttt ataccagcgg caaaaaaatg ccgatggtgg tggagggcag cggcgaatat
     7921 agctggctgc tgaccaccga agaaggaaca cagtacaaag gccatgtaac ggggggcaaa
     7981 gcgttcaatc taccgacgaa gctgccggaa ggttatcaca cgctgacact cacccaggac
     8041 gaccagcgcg cgcattgccg ggtgattgtc gccccgaaac gctgttacga accgcaggcg
     8101 ttgctgaata aacaaaagct gtggggtgcc tgcgttcagc tttatacgct gcgatcggaa
     8161 aaaaactggg gtattgggga ttttggcgat ctcaaagcga tgctggtgga tgtggcaaaa
     8221 cgtggcgggt cgttcatcgg cctgaacccg attcatgcgc tctatccggc aaatccggag
     8281 agtgccagcc catacagccc gtcttctcgc cgttggctga atgtgattta tatcgacgtt
     8341 aaagccgtcg aagatttcca tcttagcgaa gaggctcagg cctggtggca gttgccgacc
     8401 acgcaacaga cgctgcaaca agcgcgggat gccgactggg tcgattactc cacggttacc
     8461 gccctaaaaa tgacagcatt acgaatggcg tggaaaggtt tcgcgcaacg tgatgatgag
     8521 cagatggccg cgtttcgcca gtttgttgca gatcaaggcg acagcctgtt ctggcaggca
     8581 gcctttgatg cgctacatgc ccagcaagtg aaagaggacg aaatgcgctg gggctggcct
     8641 gcatggccag agatgtatca gaacgtggat tcaccagaag tgcgtcagtt ctgcgaagaa
     8701 catcgtaatg acgtcgattt ttatctctgg ttgcagtggc tggcttacag ccagtttgcc
     8761 gcctgctggg agataagcca gggctatgaa atgccgattg gcttgtatcg tgatctggcg
     8821 gttggcgtag cggaaggtgg ggcggaaacc tggtgtgacc gtgaactata ttgcctgaaa
     8881 gcgtcggttg gcgcgccgcc ggatatcctc ggcccgttgg ggcagaactg gggattaccg
     8941 ccaatggacc cgcatatcat caccgcgcgt gcctatgaac cgtttatcga gctgttgcgt
     9001 gccaatatgc aaaactgcgg cgcattacga attgaccatg tgatgtcgat gctgcgtttg
     9061 tggtggatac cgtatggcga gacggcagat cagggcgcgt atgttcacta tccggtggat
     9121 gatctgctct cgattctggc actcgaaagt aaacgtcatc gctgtatggt gattggtgaa
     9181 gatctcggta ccgtaccggt agagattgtc ggtaagctgc gcagcagcgg tgtgtactcc
     9241 tacaaagtgc tctatttcga aaacgaccac gagaagacgt tccgtgcacc gaaagcgtat
     9301 ccggagcagt cgatggcggt tgcggcgaca catgacctgc ctacgctacg cggttactgg
     9361 gagagcgggg atctaacgct gggcaaaacc ctggggctgt atccggatga agtggtactg
     9421 cgcggtctgt atcaggatcg cgaactggcg aagcaagggc tgctggatgc actgcataaa
     9481 tatggttgtc tgccaaaacg tgccgggcat aaggcatcgt tgatgtcgat gacgccgacg
     9541 ctgaaccgtg gtttgcagcg ctacattgcc gacagtaaca gtgctctgtt aggactacag
     9601 ccggaagact ggctggatat ggccgaaccg gtgaatattc ctggcaccag ttaccagtat
     9661 aaaaactggc gacgcaagct ttccgcaacg cttgagtcga tgtttgccga tgatggcgtg
     9721 aacaagttgc tgaaggattt ggacagacgg cgcagagctg cagcgaagaa gaagtagagt
     9781 gcggttgatg ccggatgcga tgcatccggc agtcagtgtt atcaaatcac catattcagc
     9841 agcagacagc ctaccagacc gcacaccgag ataatggttt ccagcatcga ccaggatttg
     9901 atggtctcgc cgatagtcag gttaaagtac tctttgaaca gccagaagcc cggatcgttc
     9961 acatgagaga aaatcacact accggaaccc accgcaataa ccatcagctc tgggctaaca
    10021 cccgtcgttg caatcagcgg tgccgcgata ccacccgcag tgattgccgc aacggttgcg
    10081 gaacccagcg cgatacgcag tacggcagca atcgaccagg ccatcagcag cggagaaatg
    10141 ttggtttcgt gcatcatgga agcaatgtat ttgtccacgc cgctgtctac cagcacctgc
    10201 ttgaacgcac cgccaccacc gatgatcaac agcatcatcg caatgatttt gatggaagaa
    10261 accagcgtgt cgttaatctg atccattgaa cgaccacggt tcagaccaaa ggtgaacatc
    10321 gcaatcagca cggcaatcag cgttgccatt accgggtcac cgaggaactc cgctaccggc
    10381 aggaaagcgt gacctttcgg caggatcatt tcggcaatcg cacgcatcgc catcagcaca
    10441 accggcacca gagaagtcca gacgctgacg ccaaagctcg gcatctcttc ttcgctgaag
    10501 gttttcgcgc tgtagagacc ttccgggatt ggcttatcga tacctttcaa cacgcgagcg
    10561 taaaccggac cggcgagaat cacggtcggg attgccagaa tagtaccgta cagcagggtt
    10621 ttacccatat cggcattgaa aatggtggca atcgcagtcg ggcccggatg cggtggcagg
    10681 aagccgtggg tcacagacag tgcagccgcc attggtacac caacatacag cagtggaata
    10741 ttcgcggaag ccgcgatggt aaacaccagc ggcagcatca gcacgaagcc cacttcatag
    10801 aacagggcaa aaccaacggt aaaaccggtt aacaccaccg cccactggat gcgcttttta
    10861 ccaaatttgg caatcagcgt ggtggcgata cgttgtgcgc caccgcagtc tgccagcatt
    10921 ttgcccagca ttgcgccaaa acccatgatc agggcaaggc taccgagcgt cccgccgaca
    10981 ccgactttga tggagtcaat aactttatcc agcggcattc cttgcattaa tccaacagca
    11041 agcgccacga ggacgagagc gatgaagccg ttcattttga agcggatcat caggagcaac
    11101 aacaagatta caccgatagc aacaatgact aatggcatga tttacctggc ctttcatttg
    11161 ttatgggtaa cgtcaatttt ctgacgacaa actctaatta tcccaatcgg gaacagagat
    11221 attgcggcac cacgactgat acccactaaa actaattatt gtagtcagat gtcaggagta
    11281 tgtttggtac ccatgtgaat gatacgggta acatttggcg tttgagaatc accagagcgg
    11341 ggtaaattta aattatgaga ggttggtcat attatcgcgg ggaaacgaag ggagggattt
    11401 gccaaagcaa tgctgtgcca acgtctggca catgttcaac gtaggcccga aatgacgctt
    11461 tagcgtcgca tcgggcaatc tacaaaagag gggataactt agtagtagga gtgttcgccg
    11521 cgctggtgtt cggtgagatc gcgcacacct ttcagctccg ggaattcgtt cagcagctgc
    11581 ttctcgatcc cttctttcag cgtcacatcg accatggaac aaccgttaca gccgccgcca
    11641 aattgcagaa tggcgtaacc gtcttcggtg atttccatca gcgaaacgcg accaccgtga
    11701 ccagcaagct gtgggttgat ctgcgactgc agcatatact ccacgcgctc catcagcggt
    11761 gcatcgtctg ccactttacg cattttggcg ttcggggctt tcagcgttaa ctgagaaccc
    11821 aactggtcgg taacaaagtc gatctctgca tcttccaggt atggtgcgct taactcatca
    11881 acatacgcgg tcagcaggtc aaatttcagg gcagtgtcgg tggcttccac ggcatccggc
    11941 ggacaataag aaacaccaca ttcagcgtta ggcgtgccag ggttaatcac aaatacgcgg
    12001 atttgtgtcc cttcttcctg atttgccagc agtttggcaa agtgcgcttg tgcagcatcg
    12061 gaaatacgga tcatagtaat ggcctaatag ttgactattt tagttggtta taatacgccc
    12121 atcatcgagg ctctacaagg ttcgacaaag gcaccagacc tggacagtcg ccgcaccatt
    12181 gcgtaaaagc aactgcgcaa tctctgcgac ggtacttccg gtggtaacga catcatccac
    12241 aatcaccata tggcgacctt gcacgggcaa ttcaagacga aaggcatttt tcaggttgcg
    12301 cttgcgcagc cgggcactga gaaaatgctg ggtcgcagtg gcccgtgtac gtgtgacggc
    12361 ttcgctatcc cattggcagt gcaaccagcg tgataacggc tgacacagca aatcgctctg
    12421 attaaatccc cgacgccagt gacgccgctg ccataacgga acgctgatga tgcgatccgg
    12481 caattgcaac ccggtggtgc gacgagcgtg taagacttcc aatagtaaca gacgtgacag
    12541 ggcgctggcg atttcactgc gccgggaaaa tttaagctgg tggatgagca gacttaacgg
    12601 cggcgcatag tcggcaaccg tgaccagtct ttgccagggc ggcggttttt gcaggcagcg
    12661 accgcaggga agatgggagt gtgtggcggg taatccacat tgtgggcata acgttttatc
    12721 tgtgcgggtg gcgcgtgaac agaccgaaca aatcccccaa tgacctaacg ccagtggcat
    12781 tcggcatagc cagcataatc ccggtactgt tagcatatgt tcatccttgt aagtcaaaag
    12841 agaacaatag cggatgaata acatttggtg gcagaccaaa ggtcagggga atgttcatct
    12901 tgtgctgctg cacggatggg gactgaatgc cgaagtgtgg cgttgcattg acgaggaact
    12961 tagctcgcat tttacgctgc accttgttga tctgcccggc ttcgggcgca gccagggatt
    13021 tggtgcgctg tcacttgctg atatggccga agccgtgctg caacaggcac ctgataaagc
    13081 catttggtta ggctggagtc tgggcgggct ggtggcaagc cagattgcgt taacccatcc
    13141 cgagcgtgtt caggcgctgg tcaccgtggc gtcgtcacct tgttttagtg ctcgtgacga
    13201 ttggccaggg ataaaaccgg acgtgctggc gggatttcag cagcaactca gtgatgattt
    13261 tcagcgtaca gtggagcggt tcctggcgtt acaaaccatg gggactgaaa cggcgcgcca
    13321 ggatgcgcgg gcgttgaaga aaaccgttct ggcgttaccg atgccggagg ttgacgtgct
    13381 taatggcggg ctggaaatcc tgaaaacggt tgatctccgt cagccgctgc aaaacgtgcc
    13441 catgccgttt ttgcgattgt atggctatct cgatggtctg gtgccgcgca aagtggtgcc
    13501 gatgctggat aaactctggc ctcgcagcaa atcatatatc ttcgccaaag cggcccatgc
    13561 gccgtttatt tcgcatccgg tcgagttttg tcacctgctg gtggcgttga agcagagggt
    13621 gtaggcaact tttgaaatgg cgagacagaa atttactggc tcgccgcagc caactcttct
    13681 tctgacaccc cggtaaagcg catgatgtcc gcaagaggga ctccggattc aagcattatt
    13741 ttggctatat gcagggcttt ggattgttcc ccctcctgcc gcaatctttc cgcaattgtc
    13801 attaaactct ccttgtgttt cggtgaacgt tcggcaacgc cgtcgataaa atcgttaaaa
    13861 cgtaccgcgt cgccggtttg cagtatgtaa ttaaacagcc ctttgatttg tctgtcatta
    13921 gcgtatccac tacttaataa gcagg
//
LOCUS       DAJTVE010000108        13896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000108 DAJTVE010000000
VERSION     DAJTVE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            232..1485
                     /gene="kch"
                     /locus_tag="OO439_003069"
     CDS             232..1485
                     /gene="kch"
                     /locus_tag="OO439_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6317372.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1540..1713)
                     /locus_tag="OO439_003070"
     CDS             complement(1540..1713)
                     /locus_tag="OO439_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6317373.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1856..3316
                     /gene="cls"
                     /locus_tag="OO439_003071"
     CDS             1856..3316
                     /gene="cls"
                     /locus_tag="OO439_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6317374.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3351..3680
                     /locus_tag="OO439_003072"
     CDS             3351..3680
                     /locus_tag="OO439_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6317375.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3733..4737)
                     /gene="oppF"
                     /locus_tag="OO439_003073"
     CDS             complement(3733..4737)
                     /gene="oppF"
                     /locus_tag="OO439_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6317376.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4734..5747)
                     /gene="oppD"
                     /locus_tag="OO439_003074"
     CDS             complement(4734..5747)
                     /gene="oppD"
                     /locus_tag="OO439_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6317377.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5759..6667)
                     /gene="oppC"
                     /locus_tag="OO439_003075"
     CDS             complement(5759..6667)
                     /gene="oppC"
                     /locus_tag="OO439_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6317378.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6682..7602)
                     /gene="oppB"
                     /locus_tag="OO439_003076"
     CDS             complement(6682..7602)
                     /gene="oppB"
                     /locus_tag="OO439_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6317379.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7688..9319)
                     /gene="oppA"
                     /locus_tag="OO439_003077"
     CDS             complement(7688..9319)
                     /gene="oppA"
                     /locus_tag="OO439_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6317380.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9738..9899
                     /locus_tag="OO439_003078"
     CDS             9738..9899
                     /locus_tag="OO439_003078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317381.1"
                     /translation="MSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            complement(10057..10704)
                     /gene="ychE"
                     /locus_tag="OO439_003079"
     CDS             complement(10057..10704)
                     /gene="ychE"
                     /locus_tag="OO439_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6317382.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11182..13857
                     /gene="adhE"
                     /locus_tag="OO439_003080"
                     /gene_synonym="adhC"
     CDS             11182..13857
                     /gene="adhE"
                     /locus_tag="OO439_003080"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6317383.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3656 a         3434 c         3230 g         3576 t
ORIGIN      
        1 gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt tatatgatgt
       61 gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa atcctttgat
      121 tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag
      181 aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac
      241 tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca cgacattctc
      301 gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc
      361 aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca
      421 aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc
      481 cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc
      541 ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt
      601 ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca
      661 tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa
      721 ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg
      781 tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt
      841 tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg
      901 cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa
      961 gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat
     1021 caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt
     1081 gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta
     1141 ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga taacgatgca
     1201 gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc
     1261 gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc
     1321 tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat
     1381 aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat
     1441 gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt aaagacgaaa
     1501 gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt agatcgcaaa
     1561 caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt
     1621 tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg
     1681 ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca ataacagaat
     1741 ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag
     1801 atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac
     1861 aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg
     1921 cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt
     1981 gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca
     2041 tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct
     2101 taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc
     2161 attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca
     2221 actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg
     2281 ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc
     2341 tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc tcgactccgc
     2401 cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga
     2461 agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg
     2521 ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat
     2581 ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc
     2641 gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat
     2701 tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca
     2761 ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat
     2821 tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgacaacgcc
     2881 ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc agcgcggggt
     2941 ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg
     3001 cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt
     3061 actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct
     3121 tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt
     3181 tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc
     3241 ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt
     3301 cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact atggatatgg
     3361 atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg
     3421 aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag
     3481 agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat gttgatttcg
     3541 atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg
     3601 aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc
     3661 atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag
     3721 cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc
     3781 ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac aggtacggaa
     3841 aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt
     3901 tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta
     3961 aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag
     4021 atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcgataaa
     4081 aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg
     4141 aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg gttcaagaat
     4201 aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat gcggatagcg
     4261 gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac
     4321 ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat
     4381 ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg
     4441 aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc aggcaacatg
     4501 accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc
     4561 cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac
     4621 ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg
     4681 cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag cattcataac
     4741 agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc ttccagcggc
     4801 ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc
     4861 ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc accttccgca
     4921 tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag
     4981 acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtcg
     5041 cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc
     5101 cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgcagta
     5161 gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact
     5221 cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt acgtgcttcc
     5281 ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg
     5341 ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac
     5401 gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca
     5461 cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg
     5521 ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca
     5581 cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt gaccgccgtg
     5641 acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtacgtca
     5701 gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt
     5761 aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg aaacaaaaca
     5821 gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt
     5881 tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca
     5941 ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca
     6001 ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac
     6061 cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt tgtccacgca
     6121 caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc agcaggatgt
     6181 tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag gagttgagga
     6241 tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac
     6301 cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc atgagtgaga
     6361 tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt
     6421 agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg
     6481 caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc agcactatca
     6541 gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc cacaagctgc
     6601 gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta
     6661 acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac
     6721 atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac
     6781 taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc
     6841 ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata
     6901 agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc gccgcatagg
     6961 taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa tcatagagcc
     7021 tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat
     7081 catgaattta agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc
     7141 aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc cggtcattgc
     7201 cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa taacgccagc
     7261 actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg cagaaacggg
     7321 aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg atggaccgaa
     7381 atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag
     7441 atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt cgccggtaaa
     7501 agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt
     7561 cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct
     7621 gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt
     7681 attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc agcggatctt
     7741 tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag
     7801 gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct
     7861 gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat
     7921 aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa gttggttcgt
     7981 tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga cgggtgtcga
     8041 ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca
     8101 atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac agcaggttga
     8161 tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt
     8221 tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg ccatcagtat
     8281 acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga
     8341 tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt
     8401 tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg
     8461 ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac gtcatgtcga
     8521 tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta
     8581 cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc
     8641 gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct
     8701 gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt
     8761 gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat
     8821 catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa
     8881 taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa
     8941 cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta tcagaccatt
     9001 tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag
     9061 cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc
     9121 ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg
     9181 aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag
     9241 ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct actaaacttc
     9301 tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca
     9361 gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta
     9421 ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca gacgatcacc
     9481 ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt
     9541 taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc ggaaagtacc
     9601 aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc tcttttattg
     9661 accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca attcaatcaa
     9721 atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt gaaatgcaca
     9781 ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt ggaattacta
     9841 atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa ataacatgat
     9901 ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga agaatatttt
     9961 tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa tgacgaagta
    10021 aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc cggggaaaat
    10081 ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata gccccataat
    10141 acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg gtgccatgcg
    10201 gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac caaacagata
    10261 gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc ctggccccgc
    10321 catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg tttctgattt
    10381 ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca tcgctatcgt
    10441 caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa aaagttgcag
    10501 aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca ggttggctgt
    10561 aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga taaagacggg
    10621 aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga agtaaacggg
    10681 aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat aatgttagcc
    10741 ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc
    10801 taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg
    10861 tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct
    10921 gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag
    10981 taatcttgct tacgccacct agaagtgacg cattagagat aataactcta atgtttaaac
    11041 tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc ataggttaag
    11101 caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt
    11161 taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta
    11221 gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa
    11281 atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc
    11341 gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct
    11401 gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac
    11461 acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc
    11521 actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc
    11581 attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc tgatatcgtt
    11641 ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct
    11701 tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact
    11761 ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat cggtgtaggc
    11821 gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct
    11881 gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt
    11941 gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg cggctatctg
    12001 ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg tgcgctgaac
    12061 gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca
    12121 gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca
    12181 catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta
    12241 gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg cctgtacact
    12301 gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt
    12361 atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa cttcaaactc
    12421 gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc tgaaaacgtt
    12481 ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg
    12541 cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa
    12601 gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat
    12661 ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc
    12721 ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac
    12781 tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag
    12841 attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt
    12901 atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc
    12961 gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac
    13021 gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga tatggcgatt
    13081 gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac
    13141 gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt
    13201 caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg
    13261 tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc gggtatcgca
    13321 tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc
    13381 catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg
    13441 aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt
    13501 cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg tactgctgct
    13561 aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg tattccgaaa
    13621 tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct
    13681 gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag
    13741 ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca
    13801 gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca
    13861 gtagcgctgt ctggcaatat aaacggcccc ttctgg
//
LOCUS       DAJTVE010000109        13787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000109 DAJTVE010000000
VERSION     DAJTVE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            448..882
                     /locus_tag="OO439_003081"
     CDS             448..882
                     /locus_tag="OO439_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCR6317384.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            974..1240
                     /locus_tag="OO439_003082"
     CDS             974..1240
                     /locus_tag="OO439_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317385.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1417..1806
                     /locus_tag="OO439_003083"
     CDS             1417..1806
                     /locus_tag="OO439_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HCR6317386.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            1803..2729
                     /locus_tag="OO439_003084"
     CDS             1803..2729
                     /locus_tag="OO439_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6317387.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            2729..2974
                     /locus_tag="OO439_003085"
     CDS             2729..2974
                     /locus_tag="OO439_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317388.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(3005..3256)
                     /locus_tag="OO439_003086"
     CDS             complement(3005..3256)
                     /locus_tag="OO439_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6317389.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            3837..4685
                     /locus_tag="OO439_003087"
     CDS             3837..4685
                     /locus_tag="OO439_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6317390.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            complement(4771..5106)
                     /locus_tag="OO439_003088"
     CDS             complement(4771..5106)
                     /locus_tag="OO439_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCR6317391.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            5339..5671
                     /locus_tag="OO439_003089"
     CDS             5339..5671
                     /locus_tag="OO439_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6317392.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            5683..8394
                     /locus_tag="OO439_003090"
     CDS             5683..8394
                     /locus_tag="OO439_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR6317393.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            8462..8893
                     /locus_tag="OO439_003091"
     CDS             8462..8893
                     /locus_tag="OO439_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HCR6317394.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            9243..9647
                     /locus_tag="OO439_003092"
                     /pseudo
     CDS             9243..9647
                     /locus_tag="OO439_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(9754..10590)
                     /locus_tag="OO439_003093"
     CDS             complement(9754..10590)
                     /locus_tag="OO439_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6317395.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(10590..10865)
                     /locus_tag="OO439_003094"
                     /pseudo
     CDS             complement(10590..10865)
                     /locus_tag="OO439_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(10940..11413)
                     /locus_tag="OO439_003095"
     CDS             complement(10940..11413)
                     /locus_tag="OO439_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6317396.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(11434..11961)
                     /locus_tag="OO439_003096"
                     /pseudo
     CDS             complement(11434..11961)
                     /locus_tag="OO439_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(12022..12837)
                     /gene="sul2"
                     /locus_tag="OO439_003097"
     CDS             complement(12022..12837)
                     /gene="sul2"
                     /locus_tag="OO439_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6317397.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         3374 a         3362 c         3772 g         3279 t
ORIGIN      
        1 cgggctgccg ctacggtccg atgtctgaaa aaccaggctt gcagcgaccg ttccggtcgc
       61 gccgcctctg tcgcgccgtg acctgcgctc cctgcacacc tgacggtgtg taaggaaact
      121 gcgcctgatg gcgcagttgt ctgcccttcc gccgccaccc ctgcggcctg tttcccgctg
      181 tggactttcc gggggctgaa gcggccacaa cacgtgtcag ccgtccaggg gggagatgta
      241 ccagccacaa aactttttgc cgcaagcgtc aaaaactttt gcgtctgccc ccggacaact
      301 gccccgtgcc gtggcagtcg ctgccccatc ggaaaggtcc actgcgggga gaaaccccga
      361 agaagtggac ggcagaaggg gctttcgccc ctccggggga gcaaccggtc ggcccggcga
      421 cctgattcag cagaaggaga cgttttcatg acagtcagca gcacgatttc cgttttttgc
      481 cgcgacgggg tgttccgtac cgtttactgc cacctgcacg gtgagccgac ctggaacggt
      541 cgcatcctgc atacccacta tgccaccggt cagcaggccg aagccctggt tgaacacggt
      601 gatatccgtt gcctcggtcc ccgttgcgac aaacccgccg ggcatacgct tcagaacccg
      661 gtggacggtg tgacggctta ttacggacgt gacagtggtt tccggatgga cagtgaggcg
      721 cgtgagtacc gttctttcag ggaggctatt gccactgaaa gcactgaaga ggtgcgcttc
      781 cattatgtgt tcatcgacgg ctactggaag gtgatgtacc gcacgccgga aggctggaag
      841 atgaaagcgc tcgcgctggc actgcgtcgc tgtccggaat gaaaaaagca gggcgataaa
      901 ccctgccttt ctccgctggc gcttccccgt acggaagtcc ggcatctcat tcactaacta
      961 tggagattat gccatgagac catcaattat cttcgcaacc gccgagtatg taaagcgtct
     1021 gcgtgaagag tgcctgcggg agaacaaacc cctgcaccgc catacccgct tcagacgcca
     1081 ggaactggca ccggatgaga ttaacccgga cgtcctggcg atgggcggtc atatcgccag
     1141 acgctgcagt gagcagaagc gggtacgtat ccccgccatg aaagtcagcg aatggggcca
     1201 cctgctccgc gcgcttgaaa ttgaacgggt ctgtcactga ttaacccctg ccgtctgcct
     1261 gtgcaggtgg caggatgtgt tcaggtactt taccaggagg aaccgtgagc aagatgaaaa
     1321 acatcacgac gtctgtgttg catggtgtct ccacaaaaaa cagaaaaggg aaacagattc
     1381 tctttcattc agggcccggg agggctggag ataacaatga agtcttgttt tgatactgac
     1441 aaagcttttc atatgtcttt tgatgaagtc actttcaacg ctgacagaat tcgctcaaaa
     1501 ctggaactat tatccgaaga actaatgccc cgttgtggtc ttgtttatga gctatatcag
     1561 cgacgtttga gtgctgctat tgataatttt gtggccacat tgccgacaga acaacatcta
     1621 ctggtcctgg aactggcccg tgaggagttt gattaccttt ccgcagaaga gattgctgaa
     1681 gaaatacgcc gtgactcaga acgcggctat tgttgtcatg gctttgaacg caattgctgt
     1741 cctctcggtt gtggggattt gggcgatgat tgctgcgatg ctgctgattt gatggaggaa
     1801 ccgtgagtaa gaagaaaaac accacaacgc ccacgccgca tgatgccgcg ttccggtcgt
     1861 tcctggcgaa tcccgacgtc gccagggatt ttctggaact gcatcttcct gcggagtacc
     1921 ggcagttgtg cgacctgtcc acgctgaagc tggaacccgc cacctttgtt gagccggacc
     1981 tgcatcagta cgccagcgat atcctctgga gcgtgaaaac caccgggggt gaagatggtt
     2041 atgtttatac gctcatcgag caccagagca cagaaaatct gtacatgcct tttcgcatgt
     2101 tacgctacag tgtggcggcg atgcagagac atctggagca gcacaaaacg ttgccactgg
     2161 tgattccggt gctgttctat cacggtgagc gcagcccgta cccgtacagc atgaactggc
     2221 tggactgttt tgagaatccg gcacttgcgg ctaaaatata cacaaagccg tttccgctgg
     2281 ttgatatcac tgtcgttgat gacaatgaaa tcatgaacca tcgccggatg gccgcactga
     2341 cgctgctgat gaagcatatc cgccatcgtg acatgatgga gctgctggac aaactcccgc
     2401 aggtcatggt ggaaatttca gacgagcagg tgcgtgttct gattcattac atcgttaacg
     2461 caggggactc tgtatcaccg gaatttatgc gggcgctggc tgagcgtctg ccgcagcatg
     2521 aggataaact gatgactatc gcagaacgtc ttgagcaaaa aggtcgccag gaaggcagga
     2581 tggaaggacg catggaagga cgcatggaag gagcgcttga aaaagccctg gctattgcgt
     2641 gccagcttca gaaaatgggg atgacgccgg agcagattaa gcaggctacc ggactttccg
     2701 atgacgaact gaagaaaatc actcactgat gagaaccaca aagcccggca aaatgccggg
     2761 cttttttgtc agcaggagca gagcaaacag catgacgatt gcagaacgtc ttatacagaa
     2821 aggtttcgac gagggtttta aacaaggggc tcttgaagtg gcgcgggaaa tagcctgccg
     2881 gcttcgggat ataggctggc cgctggaacg gattcaggag gtcacgggac tttccggtga
     2941 agaactgaaa aagctgtttc ctgatgagca gtagcctggc attcagccag gctgtgaatc
     3001 acgctcacac attccctgcc gtttccccca gttcattttc cagtcgcctg atgatatctg
     3061 ccttacgcat cccctgcatg tgaggcagca ggtcacggac tgaaagtgcc tgggtggtcc
     3121 tccagacggt gacgtccacc tgttccgcat tttcccaggc gtgccatgtt ctctgcgtca
     3181 cgccaaattc tctggccgct ttttcgcgtg accagagcat gctttttcgc cagattcgca
     3241 gttcccagcc ggtcataaaa cacctttgaa aaagtgaaaa aatttcactt tattttatca
     3301 tcataatcac caaatcaacg cgaaaaataa gcgtgaaaaa gtgaagaaat ttcacttttt
     3361 tttggttgta aatgtcgtgc ggttttctct gttttcacct gtgcgggagt ggtgtgccag
     3421 tgaccaccac atgccattgt tgtgccgtcg tttttcttca gtcgcgccgt atgcggcgct
     3481 ccctgcacac cttacggtgt gtaaggccac tgcgcctgat ggcgcagctg tctctgcctg
     3541 tctgccgccg cactgcggcc tgttctccgc agacggactt tccgggggta catacatgac
     3601 cacatcccgt ttcagtcgtc caggggagag atgtaccagc cacaaaactt tttgccgcag
     3661 gcgtcaaaaa cttttgcgtc tgcccccgga caactgcccc gtgacgtggt gagcgtcccc
     3721 cgtcggaaag gtccactgcg gggaaatccc cgaagacccg gacggcagaa ggagcttcgc
     3781 tcctccggga aatgacctgg ctgacccggc agtcgtgacg atgaggagac aatgtgatga
     3841 atcagacttt acccactgct gacctgaata ctgccggcac gacagatgtt attccgtctg
     3901 tagctatcga ccgcatcatc gcgcagcgta acgaaggtat tgcactgttc atgcaggcga
     3961 tggaatgcct ggcgacagcg cgcaagattc tgctcgatgc gtcaggtgat atttttcttt
     4021 acgggtttga agactgcgtg actgattccg ttcgtcgcat ggataagacg gaagaagcga
     4081 aaaggaatat cacccgtctt gccgaccgga aaatctggga ccgcctgatg acagatacgg
     4141 gcatgtacac cttcatgagt tcatgccagc gtgatgagtg gaacagccag ctgatgagcg
     4201 acacctgtcc tgaaatcacc ctggacaatg tactggcaac attccgccat cttaatgcca
     4261 gcaagatgca gacgtttgaa caggggctga ttgatgtcta ccggaaattg tcatgggatt
     4321 acagaaccaa taatccctgc cgtctgggta agaaaataat tattgaaaac ctactgtacc
     4381 gctggagtaa cgggcgtgtg acgctggact gcagcggacg ggaggcactg gatgacctgg
     4441 tacgtccgtt ttatctgctg gaggggcgca acgttcctga cttcaggaac agcatcgggg
     4501 cgcagtatgg tgaatttctc gggaacggtg acaatgtcgg taagctgtta gaaggggaat
     4561 attttacggt gcgtggctac cagaaaggga ccgtacacat tgtctttaag cgttctgacc
     4621 ttgttgaaaa actgaatgat attattgcac ggcattatcc tggtgcattg ccgccacgag
     4681 tctgattaaa cagaaaagcc caggtattta tgccggggct tttttttctg taatcatcat
     4741 aatctcagaa cacaatgtga cattgtcaca tcaattgttg ttcatatcga tgccatcttt
     4801 cctgcctgag tctgtctttt taggtgctgt tttagaggaa gaacggcggg atggtttttt
     4861 cgattctgct tcccgaataa tgtcacgaag tgctttctct cctacttcat atcctgcatt
     4921 tttgagcgtg tcacgaacat ctgtcagtgt ataacccttt gtccttacca ggataatgat
     4981 gtcatcacga atggcatcaa gaaaatcacg caatgttttc cgttgagcgg tgaggtctgg
     5041 caattccgat aaagcagctt ttgccagctg gatgtcatca tctgagtaaa attttttaga
     5101 cgccattggc actttctctc cgtttaatgt tgaagcctga ttttatcatc aaaaactgac
     5161 tcctggcagg gctgcgccct gcctctgccg tctgcataag actatgatgc acaaaaataa
     5221 caggctataa tggactgaaa aacgggacag gatgtgggaa tgtcggaccg cgcacgcgca
     5281 ctcagacatt ccccacctgg tcagggcttt gccccgacac cccggtaagg agcagaccat
     5341 gagtgagaag aaaacccgaa cgggcagtga gaacaggaag agaattgtaa aattcacggc
     5401 gagattcacg gaggatgagg ctgaaatcgt aagagaaaaa gcggagtcaa gtggacagac
     5461 ggtttcaaca tttatacgaa gttcttcact ggataaggtg gttaactgcc gtactgacga
     5521 ccgtatgatt gatgagatta tgcgtcttgg ccgccttcag aagcatctgt ttgtggaggg
     5581 aaaacgcact ggtgataagg agtatgcaga ggttcttgtt gccattactg aatatgtcaa
     5641 tgctctgcga agagacttaa tgggacgctg agataactgc tgatgaatgc cattattccg
     5701 cacaaaagac gtgacggcag gtcttccttt gaagacctga ttgcgtacac gtctgtgcgg
     5761 gatgatgtgc aggaaaatga gctcacagaa gacagcgaaa tcaagagtga tgtccctcac
     5821 cgcaaccgtt tcagacggct tgttgattat gcaacacgcc tgcgtaacga aaaatttgtc
     5881 tccctgattg atgtgatgaa ggacggcagt cagtgggtaa atttctatgg cgtaacctgt
     5941 ttccataact gcaattcact ggaaacagct gctgaagaga tgcagtacaa ggcggacaaa
     6001 gctgtttttt cccgtaatga tacagacccc gttttccact acattctttc ctggcctgcg
     6061 catgaaagcc cgcgtcctga gcagcttttt gactgtgtgc gtcacacact gaaatctctt
     6121 gaactctcaa aacatcagta cgtggctgcc gttcatacgg acacggataa tctgcatgtg
     6181 catgttgccg tgaatcgtgt acatcctgaa actggttata ttaactggct ttcctggagc
     6241 caggaaaaac tcagccgtgc atgtcgtgaa cttgaactta agcacggatt cgcgcctgat
     6301 aatggctgct ttgtgcatgc acccggcaac cgtattgtgc gcaaaacagc gctggtacgt
     6361 gagcgccgta atgcctggcg acgcgggaaa aagcagacgt tcagggaata tattgcgcag
     6421 atgtcgattg ccgggctgcg tgaagagccc gcacaagact ggttgtcttt gcataaacgg
     6481 ctggccagtg atggtctgta tatcacgatg caggaagggg aactggtggt gaaggatggc
     6541 tgggacagag cgcgggaagg tgttgcactc agttcgttcg gaccgtcatg gacagctgaa
     6601 aaactggggc ggaaactggg ggaatatcag cctgtaccga cagatatttt cagtcaggtg
     6661 gggacacccg gtcgttatga tccggaagcc ataaacgttg atatccggcc ggaaaaagtg
     6721 gcagaaacgg agagtctgaa acagtacgcc tgccgtcatt ttgccgaacg cttaccggaa
     6781 atggcgagaa atggtgagct ggaaagttgc ctggatgtgc acagaactct ggcaaaggct
     6841 ggattatgga tggggatcca gcacgggcat ctggtactgc atgatggatt tgataaacaa
     6901 cagacaccag tacgtgcaga cagtgtgtgg ccgttaatga cgctggatta tatgcaggat
     6961 ctggatggtg gatggcagcc tgtaccgaag gatattttta ctcaggttat acccggagaa
     7021 cgattccggg gacgtaatct tggtactcag gctgtcagtg attacgaatg gtatcgtatt
     7081 cggatgggga caggtccaca gggagcaata aagcgcgaac tgttttccga taaggaaagt
     7141 ctgtgggggt acacgactgt tcagtgtgag tcgttgattg aagatatgat cgcgggcggg
     7201 aatttcagct ggcaggcgtg ccatgagatg tttgcgcgta aagggcttat gttgcagaaa
     7261 cagcatcatg gtcttgtgat tgtggatgcg tttaatcatg agctcacacc agtgaaggcc
     7321 agcagtatcc acccggattt gacgttatcc agggctgagc cgcaggcagg gccatttgag
     7381 attgcaggtg cagatatatt tgaacgggtg aagcctgaat gtcgctacaa tccggaactt
     7441 gctgccagcg atgaagtgga gcccggcttc cggcgcgatc ctgtactgcg tcgtgaacgc
     7501 cgtgaagccc gtgcagctgc gcgggaagat ttgcgcgcgc gttatcttgc atggaaggag
     7561 cactggcgta aacccgactt acgttacgga gaacggttac gggaaattca tgctgcctgc
     7621 cgtcggcgca aggcgtatat ccgtgtgcag ttccgggatc cacaattgcg taagctgcat
     7681 taccatattg cagaagtgca gcgtatgcag gcgctgatca ggctgaagga gagtgtgaag
     7741 gaagagcggt tgtcactgat tgcggagggt aagtggtatc ctttgtccta ccggcagtgg
     7801 gtggagcaac aggcggtaca gggagacaga gctgcattat cacagttacg tggctgggat
     7861 tatcgtgatc gtcgcaaaga caagcgacga acaacgaatg ccgaccgctg cgtgattctc
     7921 tgtgaaccgg gaggaactcc actttatgaa gacactggcg ttctcgaagc ccgtctgcag
     7981 aaagatggca gtgtgcgttt tcgtgatcgg aggaatggcg agttagtctg tgtggattat
     8041 ggggaccgtg tggtgttcta ccatcatcag gacagaaatg aactggtgga taagctgaat
     8101 ctgattgctc ctgtattgtt tgatcgtgag ccaagaatgg gttttgaacc ggaaggctca
     8161 tatcaacagt ttaatgatgt atttgcggaa atggtggcct ggcataatgc agctggaata
     8221 accggaagtg gacactttgt aatcagccgc ccggatgtgg atttacatcg tcagcgtagt
     8281 gaacagtact accacgaata tatcaggcaa cagaaaagca tatccggggg gcatggcgca
     8341 tcttatgctc cagttcagga taatgagtgg acgccaccat cacctggaat gtagggatga
     8401 ggagtggata ttaaaaatct taacttgtat tagttaagat tttctttgct ctgacagagc
     8461 tatggtactg agatctatat ggattgagga gcaatatatg ggggtaaaga aaatcgttat
     8521 gctggctatt ttagtcagct ttgtggcggg atgtgcgcca ttacatcctt caaattgcca
     8581 taaaactaca gccctgggta gttgcagttc aggacgttgg gatgatcagg atgaatgggg
     8641 ggagcaagca cggggaatca gggatgcaat taatgctaaa cttgatgatc cgcaaaaatg
     8701 gaaaaggaaa aaatgcaggt tgcatatgga attctcccgg gatggcacgg cgttaaaaat
     8761 atcaaccagt aatggcgata aagcctattg cgaagcgata aagtcagcag cccataaagc
     8821 taaattcccg gccttcaaca atccggaagt ttatagagat tttcaaaaat ccggctttga
     8881 catgcgcggt tagctccacg acatatctgt agggctaaca gaagactgtc aggttggctg
     8941 attactacac tggaacctga ggtactttgc ctcaggttat gttgacatac tcaaaaatag
     9001 attcttacat tatcccggta acggcgtcct tcatcggcct gccagttacc atcaatggta
     9061 ttggtaccct cgttctttct gtctgtgatg ttaataaggg caaaattaat cagcatgtct
     9121 ggcaggagtg acgggcactg gctggcaatg tctagcaacg gcaggcattt cggctgaggg
     9181 taaaagaact ttccgctaag cgatagactg tatgtaaaca cagtattgca aggacgcgga
     9241 acatgcctca tgtggcggcc aggacggcca gccgggatcg ggatactggt cgttaccaga
     9301 gccaccgacc cgagcaaacc cttctctatc agatcgttga cgagtattac ccggcattcg
     9361 ctgcgcttat ggcagagcag ggaaaggaat tgccgggcta tgtgcaacgg gaatttgaag
     9421 aatttctcca atgcgggcgg ctggagcatg gctttctacg ggttcgctgc gagtcttgcc
     9481 acgccgagca cctggtcgct ttcagctgta agcgtcgcgg tttctgcccg agctgtgggg
     9541 cgcggcggat ggccgaaagt gccgccttgc tggttgatga agtactgcct gaacaaccca
     9601 tgcgtcagtg ggtgttgagc ttcccgtttc agctgcgttt cctgtttggg gtcgtttgcg
     9661 ggaaggggcg gaatcctacg ctaaggcttt ggccagcgat attctccggt gagattgatg
     9721 tgttcccagg ggataggaga agtcgcttga tatctagtat gacgtctgtc gcacctgctt
     9781 gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct ccatccgcgt tccaagctgc
     9841 ggaaaggcac ccataagcgt acgcctggtc gagcaggcga cgcggatcga cgtccagcgc
     9901 acgagagaat gcgtccgcca tctgtgcaat gcgtctagga tcgagacaaa ggtcgtctct
     9961 gtcagccgga tcgtagaaca tattggcggc gccaaagccc acttcaccga ccagaccgac
    10021 gggatctatc accagccagc cgcgactgga gaacatgatg ttttcatgat gcagatcgcc
    10081 atgtagccca cgcagttccg aggcattgct catcatttga tcggctataa tcgccgcgtg
    10141 gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc gcccgctgaa acaaagctgc
    10201 aaagcgatcc cggatcggga gaagggcaga aggcaggggt tcctcagatg cggcatacag
    10261 cttcgccatt agttccgctg caatttcggt cgcctggtag tcgccgtgct cggcaacgat
    10321 gtgagagagc attcgctccc cggcatattc gagcaacatc agattgttct cacgaccgag
    10381 caaccggact gctcccctcc cattgcgcca taccagatag tcggccccgc gcagttcatc
    10441 agcaatgtct tctataggtt tcaatccctt gacgattgca ggagtcccgt ctggcaatga
    10501 aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga acaggttgcg aaacgtgcca
    10561 atgagcagga aaaacaggcg gcatgaacat caaccccaag tcagagggtc caatcgcaga
    10621 tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca atacattgaa taggacagcg
    10681 aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt cggcgttagc aatcatgagt
    10741 gccaaatcgg catagcgatc tgctgttccg agccgcccaa ggtcgatcag acccgtgcat
    10801 tgaagagttt tagggtccac catgaagttc ggcatgcagg gatcaccatg gcaaacaacc
    10861 atatcgtcgc tatcaatcgc ggaaaaaggc gtagtgcgcg aagcgaacga agctttttgc
    10921 cgtcgattgc atagctttgt taaccctttt tccaaatttg atagcaatag ttaatgtttg
    10981 aagtaaagtg ttgctcaaaa acaacttcga aggtatttgg aatactcggg aagaaaacat
    11041 ccccctctgg ctcgatgtcg atcgtcgata agtggagcgt agaggccatg ggtaatgttt
    11101 ctcggtaaat ttctccgcca ccagacacta taacgtgacc ggtgaattca gctagcctgt
    11161 ccatggcctc ttcgattgac tgaaatacaa ctacattgtc atcatttgat gtccaacctg
    11221 agcgggtaac gaccgcgtat ttcctattgg ggagtgcgcc catagattca aacgtcttgc
    11281 gacccaccag aagccactga ttgtaggtca atgctttaaa aagtagctgc tcccctttcg
    11341 cggaccaggg tatgtctgga ccgcaaccaa tcacgccgtt tttcgctttc gcagctatca
    11401 atgatacttt caaggttctc atcctgggtt aacggtgcgc tcttggtcga gccgcaccgg
    11461 tagctctcgt tcgacacgag ccaaaagatc gagctgcggc gtactcttgt cctcgtccgg
    11521 taagaagtcg ggattgacgg cattgcggga caccacatca acggcgcgtc cgaacattcg
    11581 cgacagcctg cgctcaaacg gacattgatc aaccgatagg ctgtgaacag cgccaagttg
    11641 ctgccccatt gacggccacg ctttgagcaa atccgctcca gacagatcag ccgccggtac
    11701 tcccggaatt gccgttatca ccaagcatgc accctcctgt tcctcctgcc agttgatgac
    11761 ctcggggcaa gccacacctc gacctttgag ccaaatgagg cggtcacgct ctccagcgag
    11821 ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca tgcccgtcac cacgtcgaaa
    11881 aacaaaatca ccagattctc cgcctctgac aggcaaccag tcagaatgcg attcaccaaa
    11941 aaaaatatta gttcgattca atggaggttc cttcagtttt ctgatgaagc gcgaatatag
    12001 agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt tctttcagcg ccgccaatac
    12061 cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg atgaagtcag ctccacctgc
    12121 ggcggcggca agctctgcag cgagtgtcgc ggccccgaca tcccccggac cacggcctgt
    12181 gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc accggcaaat cgaagcgcag
    12241 ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt tcgggagcag cccccagaaa
    12301 aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg ataccggcac ccgtcagcgc
    12361 cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg atgtcgccag cgggtgcctc
    12421 gcgccgatct gcctgcccgt cttgcaccga atgcataacg acgagtttgg cagatgattt
    12481 cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga atatcattga gataggccac
    12541 accacgcgac aaggcatagg cttgcgtcgc gggttgataa ctgtcgagcg agacgggaat
    12601 gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc gcgatttctg tgtcggacga
    12661 aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg tcgatcacat ctgccccctc
    12721 ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc gccagatacc ggcctccatc
    12781 ggagaaactg tccgaggtta tgttgacgat gccgaaaatg atgagcgatt tattcatggg
    12841 ggcttctata ataataataa tcgagcatga gtctcatacg gatgctcggg tcgaaaggga
    12901 atccccaggc gagtaacctg tttgcggtga tccattagct gcaggagcag aatagcatac
    12961 atctggaagc aaagccagga aagcggccta tggagctgtg cggcagcgct cagtaggcaa
    13021 tttttcaaaa tattgttaag ccttttctga gcatggtatt tttcatggta ttaccaatta
    13081 gcaggaaaat aagccattga atataaaaga taaaaatgtc ttgtttacaa tagagtggga
    13141 tctgggaatt cataccaata tccatagaag tgaatctatt acgaacgtgg ggaaccctgg
    13201 caccattttt tgtgctgccc atatttgttt tcatgtataa ttcataatca aagcgttcat
    13261 cctgatttag cttaactgat taaaatccgt agattatgat tttatcagtt ttgtgacttt
    13321 atttattatg gggagcttac ccagtatttc gaaattatcg ggagaacact tgtaccatct
    13381 gtttgagcct gcaagtattt ctctgtccca tcgtctgacc ctctccccgt ttctgagtat
    13441 gcatatcata tagttttcgg gcaccatcag tgtccgggct gccagaagac tatttttaaa
    13501 cgtcagggaa gtgatattga tatcttctcc ggaattttta cgataaataa cagaccatga
    13561 gtttgtttcc gggctactaa tacacattta ttgtacctct ggtgatggat tgaaatcctg
    13621 aatcctccgc ttactatggt gagcggagga gattaataat cgcgtaaagg ttgttattaa
    13681 aatcagccat acaggtgtta tcagaatctt tttttacgac tctcttgttt tccacctgat
    13741 ggtgtctgag cgacattttt tttatggggc ggggatgcag ttagatc
//
LOCUS       DAJTVE010000110        13641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000110 DAJTVE010000000
VERSION     DAJTVE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1766
                     /gene="ldcC"
                     /locus_tag="OO439_003098"
     CDS             <1..1766
                     /gene="ldcC"
                     /locus_tag="OO439_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6317398.1"
                     /translation="NITPPFTKALFTYVKERKYTFCTPGHMGGTAYQKSPVGCLFYDF
                     FGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTNGTSTSNKIVG
                     MYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIPRREFTRDSIE
                     EKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWVPYTHFHPIYQ
                     GKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEAFMMHTTTSPS
                     YPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWFFDIWQPPQVD
                     EAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEGIPAALVAKFL
                     DERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRIKNMLPDLYAE
                     DPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAWQRQIKGEVET
                     IALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSVGQHYPGFETD
                     IHGAKQDEDGVYRVRVLKMAG"
     gene            1823..2212
                     /locus_tag="OO439_003099"
     CDS             1823..2212
                     /locus_tag="OO439_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6317399.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2277..3575
                     /gene="tilS"
                     /locus_tag="OO439_003100"
     CDS             2277..3575
                     /gene="tilS"
                     /locus_tag="OO439_003100"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6317400.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(3624..3884)
                     /gene="rof"
                     /locus_tag="OO439_003101"
     CDS             complement(3624..3884)
                     /gene="rof"
                     /locus_tag="OO439_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6317401.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(3871..4071)
                     /locus_tag="OO439_003102"
     CDS             complement(3871..4071)
                     /locus_tag="OO439_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6317402.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4237..4782
                     /locus_tag="OO439_003103"
     CDS             4237..4782
                     /locus_tag="OO439_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6317403.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            4779..5140
                     /gene="arfB"
                     /locus_tag="OO439_003104"
                     /pseudo
     CDS             4779..5140
                     /gene="arfB"
                     /locus_tag="OO439_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5214..5924
                     /gene="nlpE"
                     /locus_tag="OO439_003105"
                     /gene_synonym="cutF"
     CDS             5214..5924
                     /gene="nlpE"
                     /locus_tag="OO439_003105"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6317404.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6079..6903)
                     /locus_tag="OO439_003106"
     CDS             complement(6079..6903)
                     /locus_tag="OO439_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6317405.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(6956..8674)
                     /gene="proS"
                     /locus_tag="OO439_003107"
     CDS             complement(6956..8674)
                     /gene="proS"
                     /locus_tag="OO439_003107"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6317406.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(8785..9492)
                     /gene="tsaA"
                     /locus_tag="OO439_003108"
     CDS             complement(8785..9492)
                     /gene="tsaA"
                     /locus_tag="OO439_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6317407.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9489..9893)
                     /gene="rcsF"
                     /locus_tag="OO439_003109"
     CDS             complement(9489..9893)
                     /gene="rcsF"
                     /locus_tag="OO439_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6317408.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10011..10826)
                     /gene="metQ"
                     /locus_tag="OO439_003110"
     CDS             complement(10011..10826)
                     /gene="metQ"
                     /locus_tag="OO439_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6317409.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(10866..11519)
                     /gene="metI"
                     /locus_tag="OO439_003111"
     CDS             complement(10866..11519)
                     /gene="metI"
                     /locus_tag="OO439_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6317410.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11512..12543)
                     /gene="metN"
                     /locus_tag="OO439_003112"
     CDS             complement(11512..12543)
                     /gene="metN"
                     /locus_tag="OO439_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6317411.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            12731..13306
                     /gene="gmhB"
                     /locus_tag="OO439_003113"
     CDS             12731..13306
                     /gene="gmhB"
                     /locus_tag="OO439_003113"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6317412.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3319 a         3464 c         3625 g         3233 t
ORIGIN      
        1 ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag cggaagtaca
       61 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgcc
      121 tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt tcggtcaccg
      181 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
      241 ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
      301 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
      361 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
      421 gtaatgcgtt ggggattctt ggtgggatcc cgcgccgtga atttactcgc gacagcatcg
      481 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg attaccaact
      541 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
      601 cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc taccagggga
      661 aaagtggtat gagcggcgag cgtgttgcgg gaaaagtgat cttcgaaacg caatcgaccc
      721 acaaaatgct ggcggcgtta tcgcaggctt cgctgatcca cattaaaggc gagtatgacg
      781 aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt tatcccattg
      841 ttgcttcggt tgagatggcg gcggcgatgc tgcgtggtaa tccgggcaaa cggctgatta
      901 accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggttg cgggaagagt
      961 ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc gaatgctggc
     1021 ccgttgcgcc aggcgaacag tggcacggct ttaacgatgc ggatgccgat catatgtttc
     1081 tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc aatatgagcg
     1141 aggaggggat cccggcggcg ctggtggcaa aattcctcga cgaacgtggg atcgtagtag
     1201 agaaaaccgg cccttataac ctgctgtttc tctttagtat tggtatcgat aaaaccaaag
     1261 caatgggatt attgcgtggg ttgacggaat ttaaacgctc ttacgatctc aacctgcgga
     1321 tcaaaaatat gctacccgat ctctatgctg aagatcccga tttctaccgc aatatgcgta
     1381 ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt cccggtttga
     1441 tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag gcatggcaga
     1501 gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt agagtatcgg
     1561 caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga gaaatgctga
     1621 ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta gggcaacatt
     1681 accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc gtttaccgcg
     1741 tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga gtaacgtgct
     1801 gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata ttgcgattat
     1861 tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt tcacgctgca
     1921 aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc ttaatgggca
     1981 atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc gaccggaagc
     2041 ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc gatgcggcag tggcgcacct
     2101 tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc aaaaacgctt
     2161 cacgttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt aaggcttgtc
     2221 atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc accatcatga
     2281 cactcacgct caatagacaa cttctcacct cacgccagat tctggtggcc tttagcggcg
     2341 ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa aatccgggcg
     2401 tcactctacg cgctatccat gtgcatcatg gtttaagtgc taatgccgat gcctgggtta
     2461 ggcattgcga gaacatctgc caacagtggc aggtgccgct ggtggtcgag cgcgtgcaac
     2521 ttgcgcaaga aggactgggc attgaggccc aggcgcggca ggcgcgttat caggcatttg
     2581 ctcgtaccct gttacccggt gaagtgctgg tcaccgcgca acatctcgac gatcaatgtg
     2641 aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg gctatggggg
     2701 aagtttcgga atttgccgga acacggctta tccgcccgtt gcttgcccgc acgcgggggg
     2761 aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa agtaatcagg
     2821 acgactcata cgatcgtaac tttctgcgcc tgcgtgtagt gccgttattg cagcagcgtt
     2881 ggccgcattt tgccgaagca acggcccgca gcgccgcact ttgtgctgaa caagagagtc
     2941 tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag gggacgctgc
     3001 agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatc cgccgctggc
     3061 tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcggatc tggcaggaag
     3121 tggcgctggc gcgggaagat gcctccccct gtttacgttt tggcgcgttt gaaatacggc
     3181 gctatcagtc gcaactgtgg tggattaaat ccgtcactgg gcaaagcgaa accattgtgc
     3241 cgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaagt gtacagctta
     3301 ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag
     3361 cgccaggatt gctgcatatt gtcgggcgta acggcggacg taagctaaag aaaatctggc
     3421 aagagctggg cgtgccgccg tggctacgtg acaccacgcc actgctgttt tatggcgaaa
     3481 cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa ggtgagaatg
     3541 gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa gacgcatcaa
     3601 acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc gatttccggg
     3661 tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc ggcctcgacg
     3721 accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg caatttttcg
     3781 ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc aagattatcg
     3841 taatcatcac aattgattgg ttgatacgca tcattcattg acataatcgc tcaccagtaa
     3901 gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc
     3961 catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc
     4021 gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc
     4081 tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt
     4141 cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg cacgtattcc
     4201 gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat
     4261 aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg
     4321 ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcctgct ggcgtggctg
     4381 aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatta tgagccggaa
     4441 gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg gctgccggat
     4501 gagcggcgga ttaagaaagc ctgcacccag gccgcaaaag tggcgctgtt tacctataat
     4561 agccgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca gtttgccaat
     4621 ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt
     4681 accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga tgataagaat
     4741 aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg
     4801 ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc gcgggcgggc
     4861 agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg gcgtccagcc
     4921 tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc agcatgatgg
     4981 cgtaattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagcggcgct
     5041 ggcccggctg gtggcagtga ttaaagattt aacaacagaa caaaaagccc gacgacccac
     5101 gcggcccacc cgtgcatcga aagagcgcag gctggcatag aaagcacaaa aatcaagcgt
     5161 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
     5221 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
     5281 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactaaaa ccgatgccgc
     5341 aaagttggcg cggcgtgctg ccgtgtgccg attgcgaagg gatcgaaacc tctctgttcc
     5401 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
     5461 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
     5521 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
     5581 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
     5641 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
     5701 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
     5761 acctggctgc gcgcggtcac agtgaaaaac cggtgttact gtcagtagaa ggtcacttta
     5821 cgcttgaggc taatccggat actggtgcgc caaccaaagt attagcgccc gatacggcgg
     5881 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
     5941 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
     6001 ttttgaatta gcacgatttt gtaggccgga taaggcgttc acgacgcatc cggcattttg
     6061 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
     6121 ccctggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
     6181 gccagtacgc atatgcatca gatcggcagg gctaatccag cctgattgcg ccagcgtaag
     6241 cggatgcccg gctttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
     6301 gtctccttcg cccacactgc ttaactgcgc tttcgccagg ccgctgacgc actgttggcg
     6361 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
     6421 ttcgacaatg ccgcgatagt tataactgct atctttgatt ttattggcaa acgcggtgat
     6481 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
     6541 cttcatcgct tttttaaggg aaacgatctg gacgccagcg cctgtcgctt ctgcaacgtt
     6601 attatcaccc agatagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
     6661 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtac
     6721 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
     6781 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
     6841 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
     6901 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
     6961 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
     7021 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
     7081 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
     7141 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
     7201 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
     7261 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
     7321 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
     7381 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
     7441 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
     7501 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
     7561 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
     7621 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
     7681 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
     7741 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
     7801 ggaagctgct gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
     7861 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
     7921 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
     7981 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
     8041 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcttgc acggcgcgga
     8101 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
     8161 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
     8221 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
     8281 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
     8341 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
     8401 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
     8461 gcatcgacac ctcgattgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
     8521 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
     8581 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
     8641 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
     8701 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
     8761 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
     8821 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
     8881 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
     8941 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
     9001 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
     9061 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
     9121 cagactgccg agcttcagaa tcacgctgtc tttatggcaa acaacctctt tcagctctac
     9181 cagcgacata ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
     9241 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
     9301 atgaaagacg aaaaggatcc ataaatggct gaacgcttcc aggccgcgaa cggcgtcggc
     9361 ctggttgtag ggagcaatga gatgcagttc gccgttggcg ctttttacca gaccaggctg
     9421 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
     9481 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
     9541 cagcctagcg taccgctggt gacttcgcag ctatgcagta ataccgcatt ggctttcatt
     9601 ttagaggcgt tgatttgcat ccgcttacgc gcggttggaa tgctcggcgg agagtcctga
     9661 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
     9721 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
     9781 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
     9841 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
     9901 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
     9961 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    10021 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    10081 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    10141 ttcacgtacg gggagtcttt atcttcgaca aagataccgt ctttcgctgg agtcaggcca
    10201 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagat
    10261 cgaggcagct gcggtgcttc cagctcaaca attttcagat tttttgggtt ctcaacaaca
    10321 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    10381 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    10441 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    10501 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    10561 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    10621 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    10681 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    10741 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    10801 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    10861 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    10921 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    10981 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    11041 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    11101 ggtaacagca ctttacggac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    11161 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    11221 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    11281 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    11341 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    11401 acataaagca gaacgccaac cggcagacca atcacaaagc caaaaaatcc ggatacgaag
    11461 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    11521 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    11581 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    11641 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    11701 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    11761 tggctccgct tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    11821 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    11881 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    11941 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    12001 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    12061 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    12121 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    12181 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    12241 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    12301 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcttggccat cgaccagcac
    12361 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    12421 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    12481 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    12541 cattgattat ttattatcgt cattaagtta gtcgtggcat ctcgaatgcc tgaaacgggc
    12601 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    12661 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    12721 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    12781 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    12841 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    12901 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    12961 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    13021 gggtagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    13081 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    13141 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    13201 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    13261 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgagcg
    13321 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    13381 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    13441 aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac
    13501 agtgagctga aagctgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    13561 cactcgaaga tacggattct taacgtcgca agacgaaaaa tgaataccaa gtctcaagag
    13621 tgaacacgta attcattacg a
//
LOCUS       DAJTVE010000111        13426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000111 DAJTVE010000000
VERSION     DAJTVE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..131)
                     /locus_tag="OO439_003114"
                     /pseudo
     CDS             complement(1..131)
                     /locus_tag="OO439_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            132..677
                     /locus_tag="OO439_003115"
                     /pseudo
     CDS             132..677
                     /locus_tag="OO439_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(719..1720)
                     /gene="gap"
                     /locus_tag="OO439_003116"
     CDS             complement(719..1720)
                     /gene="gap"
                     /locus_tag="OO439_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6317413.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            1909..2439
                     /gene="cybB"
                     /locus_tag="OO439_003117"
     CDS             1909..2439
                     /gene="cybB"
                     /locus_tag="OO439_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6317414.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            2684..2857
                     /locus_tag="OO439_003118"
     CDS             2684..2857
                     /locus_tag="OO439_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317415.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2929..3078)
                     /gene="hokB"
                     /locus_tag="OO439_003119"
     CDS             complement(2929..3078)
                     /gene="hokB"
                     /locus_tag="OO439_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6317416.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3477..5117
                     /gene="trg"
                     /locus_tag="OO439_003120"
     CDS             3477..5117
                     /gene="trg"
                     /locus_tag="OO439_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6317417.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5155..6078)
                     /locus_tag="OO439_003121"
     CDS             complement(5155..6078)
                     /locus_tag="OO439_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317418.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6295..7638
                     /locus_tag="OO439_003122"
     CDS             6295..7638
                     /locus_tag="OO439_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6317419.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7863..9518
                     /gene="opgD"
                     /locus_tag="OO439_003123"
                     /gene_synonym="mdoD"
     CDS             7863..9518
                     /gene="opgD"
                     /locus_tag="OO439_003123"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6317420.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            9658..9882
                     /locus_tag="OO439_003124"
     CDS             9658..9882
                     /locus_tag="OO439_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6317421.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9945..10481
                     /gene="rimL"
                     /locus_tag="OO439_003125"
     CDS             9945..10481
                     /gene="rimL"
                     /locus_tag="OO439_003125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6317422.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10476..11456)
                     /locus_tag="OO439_003126"
     CDS             complement(10476..11456)
                     /locus_tag="OO439_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6317423.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11580..12572
                     /gene="tehA"
                     /locus_tag="OO439_003127"
     CDS             11580..12572
                     /gene="tehA"
                     /locus_tag="OO439_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6317424.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12569..13162
                     /gene="tehB"
                     /locus_tag="OO439_003128"
     CDS             12569..13162
                     /gene="tehB"
                     /locus_tag="OO439_003128"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6317425.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3420 a         3125 c         3399 g         3482 t
ORIGIN      
        1 taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc
       61 catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga
      121 ccgtagccat tgatcagttc gtcaatcggc tgggtgaagc gatgcaggcg gttaaatttg
      181 gtaaccccgc tgaacgcaac gacattgcga tggggccgtt gattaacgcc gcggcgctgg
      241 aaagggttga gcaaaaagtg gcgcgcgcag tagaagaagg ggcgagagtg gcgttgggtg
      301 gcaaagcggt agaggggaaa ggatattatt atccgccgac attgctgctg gatgttcgcc
      361 aggatatgtc gattatgcat gaggaaacct ttggcccggt tctgccggtg gtcgcatttg
      421 acacgctgga agaggctatc tcaatggcta atgacagtga ttacggcctg acctcatcaa
      481 tctataccca aaatctgaac gtcgcgatga aagccattaa agggctgaag tttggtgaaa
      541 cttacatcaa ccgtgaaaac ttcgaagcta tgcaaggctt ccacgccgga tggcgtaaat
      601 ccggtattgg cggcgcagat ggtaaacatg gcctgcatga atatctgcag acccaggtgg
      661 tttatttaca gtcgtaatga gtaaaagagg cggaggtttt ttcctccgcc tgtgcgcgtc
      721 agagtttagc gaatttttcg agggtgcgaa taagctgcgt gacgaagcca tattcgttat
      781 cgtaccaggc gaccgttttc accagttgta aatcgcccac ggcggtaatt tccgtttgcg
      841 tggcatcaaa caccgaaccg aaatggctgc caatgatatc ggaagagact atttcttcat
      901 cggtataacc aaatgactcg ttattggtgg ttgcttgttt aagtgcgtta ttcacctctt
      961 cggcagtcac tttttttccg agaatcgata ccagttcagt gaccgaacct gttttcaccg
     1021 gcacgcgttg cgcatgacct ttcagtttgc cgctcagttc cgggatcacc agaccaatgg
     1081 cttttgccgc ccccgtagtg tggggaatga tattttctgc cgctgcgcgt gaagcacgta
     1141 aatctttacc acgtgggcca tccaccagtg actgggtgcc agtataggca tgaatggtcg
     1201 tcatcgtgcc gacttctatc ccgaaactgt catgcaaggc tttggccatc ggcgcaagac
     1261 agttagtggt gcatgacgcc acggaaacaa tggtgtcgtt gccatccaga gtgtcgtcat
     1321 tgacgttata aacgatagtt ttcatttcac cggcaggggc ggaaatcaac accttcttcg
     1381 caccagcatc aagatgtgcc tgcgatttct cggcggaggt ataaaagcca gtacattcga
     1441 caatgatttc tgcacctttc gctttccacg gaatattttt agcctctttt tcggcgtaaa
     1501 ccgcgatact tttcccatca acgataagtg aatcttccgt aaaatcaacg ctccagggga
     1561 atggtccgta gtttgaatca tgtttcagca ggtaggcgag aatttttggg gaagtgagat
     1621 cattaatagc gacaacgtct atgttgcttt tgacttcaag taatcgacgc aacaccagtc
     1681 gaccgatacg accaaaaccg ttaataccaa ctttactcat ggttttctcc tgtcaggaac
     1741 attcggatga aaattgatcc tttccaggct tagaccagga ttgtgggatg ggcaatctcc
     1801 attcttacag tgaaacgtaa cgtactgaaa acgggtgaac aatatttaat gaaattttga
     1861 gaaaagcccg ttatgttaac ggaaaattat gttaaagcag gaaatgttat ggaaaataaa
     1921 tactcaaggt tacaaatcag cattcactgg ctggtctttt tgctggttat cgcagcgtat
     1981 tgcgcaatgg agtttcgtgg tttcttccca cgtagcgatc ggccactcat caacatgatt
     2041 catgtttcct gcggcatctc aattctcgtg ctgatggtcg ttcgtctttt gttaaggctg
     2101 aaatacccaa ccccgccgat tatacctaag ccaaaaccga tgatgacggg actggcgcat
     2161 ttgggacatt tggtgatgta tctcctgttt attgcgctgc cagtgattgg tttggtgatg
     2221 atgtataacc ggggcaaccc gtggtttgcg tttggtttga cgatgcctta cgcttcagag
     2281 gccaatttcg aacgggttga tagcttaaag tcgtggcatg agacgctggc gaatctggga
     2341 tattttgtca tcgggttgca cgctgcggct gcactggcac accactattt ctggaaggac
     2401 aacacacttc tacgcatgat gccgcgtaaa cgttcctgaa ggatatttca agaaaatgcc
     2461 tgtactaaaa ccgacccgtg gtacaggcga agaatacggg tctacatcgg aagcgcctat
     2521 tatatttatt tgtatgacaa ataaaaccat aatccttgcc catacgtcca tctggcttat
     2581 ttttaatcaa ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata
     2641 acgttcaaat cgaacataag attcgataaa ccatggaggt tatatgaaaa aactggcact
     2701 tattttgttt atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc
     2761 tggttcgaaa ggggggatct cacactgtac ggcaggcggc aaatttgtct gtaatgatgg
     2821 ttctattagt gcatcgaaaa aaacatgcac taactgaagt gtaaaagggg tgccatgaga
     2881 aaatgaatct gctagtcaga tgcgcgggga aaatccccgc gcttgccctt acctggacgt
     2941 gcaggccatg agcgcagcaa cctccttatc accgtcccgg aaccgcagtt cgtagagcgt
     3001 ttgtcgggtc aggagtgtga atgtcagaat cgtaatgcag ataatgagca gacacactac
     3061 cagagggttg tgcttcatag cctttctcct tgccggatgg cgggtaagag gctaagatct
     3121 gaattgctag gttcattcgt tggcctcggt tgatagaaat atcggtcggg gccttcgtct
     3181 ttctgattcc cggttagcct gaaaacagaa agtctcaggc acccgcaggc atcctatgag
     3241 gtttccttag ggacgaaaat aatcacttca cgaaattgcg tgctgttttc cagaattttt
     3301 cgtcattcgg gttagccagt ttagccattc gttactctct tcattccaat agcattaatt
     3361 ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg tttttggtca taagtaatta
     3421 ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg acgagagaca acggtaatga
     3481 atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt ccgttatttg
     3541 cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg gctggctatt
     3601 tcctctggca ggccgatcgc gatcagcgtg atgttactgc ggagattgag attcggaccg
     3661 ggttagcgaa cagttcagat tttttgcgtt cagcccggat caatatgatt caggccgggg
     3721 ctgcgagttg tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg
     3781 agattaaaca gtcgcagcaa gggtatcgtg cttatcagaa tcggccggtg aaaacacctg
     3841 ctgatgaagc cctcgacact gaattaaatc aacgctttca ggcttatatc acgggtatgc
     3901 aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc gattatcaat catgaaagtg
     3961 agcagatccg accgctggat aatgcttata ccgatatttt gaacaaagcc gttaagatac
     4021 gtagcaccag agccaaccaa ctggcggaac tggcccatca gcgcacccgc ttgggtggga
     4081 tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac gctgataaca tttatggtgc
     4141 tacgtcggat cgtcattcgt ccactgcaac atgccgcaca acggattgaa aaaatcgcta
     4201 gtggcgatct gacgatgaag gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaaatc
     4261 gtcatttaca gcaaatgcag cattcactgg ggatgacagt agggactgtt cgacagggtg
     4321 cggaagagat ttatcgtggc accagcgaaa tttcagctgg caatgcggac ctgtcatctc
     4381 gcaccgaaga acaagcggcg gctatcgaac aaactgccgc cagcatggag caactcactg
     4441 cgacggtgaa acagaatgcg gataacgcgc atcatgccag caaactggcg caagaggctt
     4501 ctattaaagc cagcgatggc gggcagacgg tttccggtgt agtaaaaacg atgggcgcta
     4561 tctccacgag ttcgaagaaa atttctgaga tcaccgccgt catcaacagt attgctttcc
     4621 agacgaatat tctggcactg aatgctgccg ttgaagccgc gcgagcgggt gagcaagggc
     4681 gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc aagtcgcagc gctcaggcgg
     4741 cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt aattgacctg gggtcggatg
     4801 aggtggcaac ggccgggaaa accatgagca ctattgttga tgccgtcgcg agtgtcacac
     4861 atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag tagaggcata acgcaggtta
     4921 gccaggcgat ttctgaaatg gataaggtga cgcaacagaa tgcttctctg gtagaagagg
     4981 cctcagcggc ggcggtgtcc cttgaagaac aggcggcacg attaactgag gcggtggatg
     5041 tattccgtct gcacaaacat tctgtgtcgg cagaacctcg cggagcgggt gaaccagtta
     5101 gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca gatcccttca cctttcagaa
     5161 cggcattgat tttcgaatag cgttaatcat caactggcaa ccagaagaga acgtcgcatc
     5221 tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt cccggaacag gcagggccac
     5281 cagcgtggca tgacgcaggt cgtcttttac agcgccagaa gggacaaacc acacgtaatc
     5341 gtattcaacc gtaagttgac gagatagcga agcagacagc gtttcgatac aacccgaagg
     5401 aattttacag ccctggctct gcactaatgc atctgaatgc tggcgtggcg cagtgccttc
     5461 tggtgataca acgaccggcc attccagcac ccggcttagc gttacgttct cctgaagtag
     5521 cgggtgatta gggcggacaa ccagcttcag cgattcaaga aacagcagtt cgtaattaag
     5581 cccggtcatc agttcaggat ctgacatccg accaatgccg atatcgattt ccccggtttt
     5641 taaacccgcc agaatcatag ggttactcat tgtcgcaact tgcaaggtcg tctctttttg
     5701 ttgctgatga aactgaccta taaccgaagg taatatcccc agtgccgcag taggtagtgc
     5761 accaaccctg acgacatcat tattaagacc ttctttacga tgaagcgact gtccggcagt
     5821 gttgatggcg tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag
     5881 ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt
     5941 caatgtctta gagagcgcag gttgactcaa attaagggtt tcagccgcgc gccccaaagt
     6001 tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa
     6061 cagactattt ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt
     6121 tgtttgatta tgataacttg attgcaaaat attattaaca attaaagcaa ttatgttaca
     6181 gcaaaatgga taatattgat gttttcgcgg cgagatcaca gtttgtaaat tcttcccgcc
     6241 agagtgaatg cggttaccta cactccagat tactgaccac tggaggcaga cactatggcg
     6301 aacagcatca cggcggatga gattcgggaa cagttttcgc aggcaatgtc agccatgtac
     6361 cagcaagaag ttccgcaata tggcacgctg ctggaactgg tagctgatgt gaatctggct
     6421 gtgctggaaa acaatcctca actgcacgaa aaaatggtaa atgcagacga gctggcgcga
     6481 ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg cacaagagct tgctactctt
     6541 cggcggatgt ttgccattat ggggatgtac ccggtgagct attacgatct ctcgcaggca
     6601 ggggtgccgg tacattcgac agcatttcgg cccattgatg atgcttctct ggcgcgtaat
     6661 cccttccgcg tttttacctc cttactccgc cttgagctta tcgagaacga aattttgcgc
     6721 cagaaagcgg cggagattct gcgtcagcgc gatatcttca ccccacgttg tcgacaactg
     6781 ttagaggaat atgagcagca gggcggtttt aacgaaacac aggcacagga gtttgtgcag
     6841 gaagccctgg aaacgtttcg ctggcaccag tcagcaacgg tagatgaaga aacctatcgc
     6901 gccttgcaca acgaacatcg gttgattgct gatgtggtct gttttcctgg atgccatatc
     6961 aaccacctga cgccacgtac gctggatatt gaccgggtgc agtcgatgat gcctgaatgc
     7021 ggaattgaac ccaaaattct catcgagggg ccgccgcgcc gcgaggtacc gattttacta
     7081 cgccagacca gctttaaagc actggaagag acggtgttgt ttgcggggca gaaacagggc
     7141 acgcataccg cgcgctttgg tgaaattgag cagcgtggcg tggcattaac gccgaaaggg
     7201 cgacaactgt atgatgatct tctgcgtaac gctggaaccg ggcaggataa tctcactcac
     7261 caaatgcatt tacaggaaac cttccgcact tttcctgaca gtgagttttt aatgcgtcag
     7321 caagggttgg catggttccg gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg
     7381 attcatcctg gagacgatcc acagccctta attgaacgtg gttgggtagt ggcgcaaccc
     7441 atcacctatg aagatttctt gcccgttagc gcggcgggga tcttccagtc aaatctgggt
     7501 aatgaaacgc agacacgcag tcacggtaat gccagtcgcg aagcatttga gcaggcgttg
     7561 ggttgtccgg ttttggatga gttccagctt taccaggaag cggaagaacg cagtaaacgt
     7621 cgctgtggtt tgctttaaat tctgaccatc cgcctttgca aaaatttgcc tgattttaca
     7681 aacgaatcag gctcatccca tcgacataaa aaaaatgccg atttatgcat attctctcag
     7741 ttcaacaatt ggattattaa taaatattgt ctagagtgag cggtcataaa taagcacttt
     7801 cttgccgctg aaaacgacca gcgcgggacc attcacaaca ccagaaggac tcactttcag
     7861 gtatggatcg tagacgattt attaaaggtt caatggctat ggccgccgtg tgcggtacca
     7921 gcggcattgc ttctcttttt tctcaggcgg cattcgcggc agattctgat attgccgacg
     7981 ggcaaaccca gcgttttgac ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa
     8041 cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct ggcgacaatg acgccgcagg
     8101 cttataacag tattcaatac gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc
     8161 aactggacgc tcagttcttc catatgggaa tgggattccg tcgccgcgtt cgtatgtttt
     8221 ctgtagatcc agcaacacat ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat
     8281 acaacgatgc aggtgttgat accaaacaat tagaagggca aagcgatctc ggctttgccg
     8341 gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga tgtagtatca tttctcggcg
     8401 cgagttattt ccgcgccgtt gatgatacat atcaatacgg tttgtcggcc cgcggcctgg
     8461 cgatcgacac ttacaccgac agtaaagaag agttccccga ctttaccgcc ttctggtttg
     8521 atacggtaaa accgggggca actaccttta ccgtttatgc gttgctcgat agcgccagta
     8581 ttactggtgc ctataagttc actatccatt gtgagaaaag tcaggtgatt atggatgtgg
     8641 aaaatcacct gtatgcgctc aaagacatta aacagctggg cattgcgccg atgaccagta
     8701 tgttcagctg cggtactaat gaacgtcgga tgtgcgacac cattcatccg caaattcatg
     8761 actctgatcg tctgtccatg tggcggggca acggcgagtg gatttgccgt ccgctgaaca
     8821 atccgcaaaa attgcagttc aatgcttaca ccgacaacaa cccgaaaggg tttggtttat
     8881 tgcaactgga tcgtgacttc tcccattatc aggacattat gggctggtat aacaaacgcc
     8941 caagtttgtg ggtggaaccg cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa
     9001 tcccaacaac gggcgaaacg ctggataaca ttgtctgctt ctggcagcca gaaaaagctg
     9061 taaaagcagg tgatgagttt gcattccagt atcgtctgta ctggagtgcg caaccgcctg
     9121 ttcattgccc attagcgcgc gttatggcga cgcgtaccgg catgggcggt ttcccggaag
     9181 gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg tcgttttgcc gtcgatttcg
     9241 ttggtggtga tctgaaagct gccgcgccaa aaggcattga gccggtgatt acgctttcca
     9301 gtggggaagc gaagcaaatc gaaattctct atattgaacc catcgatggt tatcgtattc
     9361 agtttgactg gtatccgact tcggactcta ctgatccggt cgatatgcgg atgtatctac
     9421 gttgtcaggg ggacgctatc agtgaaacat ggctgtatca gtatttcccg ccagcgccgg
     9481 ataaacgtca gtatgttgac gaccgcgtga tgagttaatc gttttttctt cggcaccttc
     9541 ttcgggaggt gccgtctggt taaacacgat cccgctcgca tttttcccta agttaaatga
     9601 gtaatctgat ggtgtgtatt tcagatacac cttgtcagcc actaacaggg agtgcgtatg
     9661 tttccagaat accgagattt aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc
     9721 ttgttcgata aacacaataa acttgatcat gaaattgcca gaaaggaagg ttccgacggt
     9781 cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag
     9841 atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt aaactttcct aagccgccag
     9901 ccaggcggct tttttaacaa cagcatggat tgactggaga taagatgact gaaacgataa
     9961 aagtaagcga atcacttgaa ttacatgctg ttgcggaaag tcacgtcaca cctctttatc
    10021 agttaatctg taaaaataaa acctggttac agcagtcgct aaactggccg cagttcgttc
    10081 agagtgaaga ggacacgcga aaaacggtgc agggtaatgt gatgttgcat caacgcggct
    10141 atgccaaaat gttcatgatt ttcaaagaag atgaacttat cggcgttatc tcgtttaatc
    10201 gtattgaacc actgaataaa actgctgaaa tcggctactg gctggacgaa tctcatcagg
    10261 ggcaggggat catttctcag gcgctgcagg cattgattca tcattacgct cagtctggtg
    10321 aacttagacg cttcgtgatc aaatgtcggg tggacaatcc gcaaagcaac caggtcgctt
    10381 tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc tgagttcctg aatgatgcct
    10441 atgatgatgt gaacttatac gcgcgtatta tcgattcata ataaccctgc cagcggcgtt
    10501 cgcgtaatgc gctcctcgcc gttaatgact tttggaccac ggacatgaac cgtgtcgcca
    10561 tcaaaggctt caacgacggc gtggtcggtt aactccacat ggttttcaat tatcaccgca
    10621 cctgaaatac ggctctcacc ttgtattaca acgtgctcgt cgagcagaat cggtccacca
    10681 cgtaccacgg catttccgcc aatcaagaca tgatgtttta acacacaatt accttccaca
    10741 atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg
    10801 ccagctttca cctgcgcgtg accgtagact tttgcgcaat cgcatagcca gacgttgttc
    10861 tcttcattac cttcaacact ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca
    10921 taccggacga cggcatcgcc ataaatttgt gcctggtgga caatacgcga ggcgctaacc
    10981 cttgcgcgat cataaatttg gagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg
    11041 acgatcatag aatgttgatc aattaaggcg tgaccaaata ttctacattg cccgtaaact
    11101 aatgagtcac gaatggtgac actgtcactt atatatgcac cctggctgat ttcactatta
    11161 tcgatccaga cgttatccgt tgcgtaaacc tctccccaca gcacgctggt gccggtgata
    11221 cgcgtattac cagaaatcac cgttccgccg aacgcaatag cattctggtc gtaaatccag
    11281 caatttcctt cctgtgctaa caccgtttcg cgatcgatcc agccgccagc ggttcctgct
    11341 atcacatcgt taaaatcgct aatggcgata atctgccgta ataacacact ttttttagtg
    11401 ccatcttcct gataactaaa ggctcgctgt tcttcactta gacgatattt gcgcatagcg
    11461 ttttctcaca ggtgacttac tataaccgta gcaaattctg cggctctggc tatgctcgag
    11521 aaaattccat aaaatgcatt tcaaatatac tttataaatt aaacaaaatg agtaagaaga
    11581 tgcagagcga taaagtgctc aatttgccgg caggctactt tggtattgtg ttggggacga
    11641 tagggatggg atttgcctgg cgctatgcca gccaggtttg gcaggtcagc cactggttag
    11701 gggatgggct ggtgattctg gcgatgatca tctggggatt attaactagc gcatttatta
    11761 cccgactcat acgctttccg catagcgtgc tggcggaagt tcgccatcca gtgctgagca
    11821 gttttgtgag tttgtttccg gcaacgacga tgctggtggc gattggtttt gttccgtggt
    11881 ttcgcccact ggcggtgtgc ctgttcagtt ttggtgtcgt ggttcagttg gcttatgccg
    11941 cctggcaaac tgcgggatta tggcgcggat ctcaccctga agaagctacc acgcctggac
    12001 tgtatctgcc gacagttgcc aacaacttta tcagcgcaat ggcctgtggt gcgttgggct
    12061 acaccgacgc cggtctggtg tttttaggcg caggcgtttt ctcatggcta agtcttgaac
    12121 cggtgatctt gcagcgtctg cgcagttcgg gagaattacc cacggcactg cggacatcac
    12181 tcggcattca gctcgctcct gcgctggtgg cctgtagtgc ctggttgagc gtcaacggcg
    12241 gcgagggtga tacgctggcg aaaatgcttt ttggttatgg actgctgcaa ctgctgttta
    12301 tgctacgtct gatgccatgg tatctctccc agccatttaa tgcttcattc tggagtttct
    12361 cgttcggcgt atctgcactg gcaaccaccg gtttgcatct ggggagtggc agcgaaaatg
    12421 gatttttcca tacgctggcg gtaccgctgt ttatctttac caattttatt attgcaatac
    12481 tgctcatccg tacttttgcg cttctgatgc agggaaaatt gttagtcaga accgagcgcg
    12541 ccgttttaat gaaagcagag gacaaagaat gatcattcgt gacgaaaact attttactga
    12601 taaatatgaa ttaacccgca cacactctga agtactggaa gcggtgaaag tggttaaacc
    12661 gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac agtctttacc tggcagccaa
    12721 tggttatgat gttgacgcat gggataaaaa tgccatgagt atcgccaatg tagagcgcat
    12781 taaatccatt gaaaatctgg ataatttaca cacccgagtc gttgatctga ataacctcac
    12841 atttgataga cagtacgatt ttattctttc gactgtggta ctgatgttcc ttgaggctaa
    12901 aaccatccca gggctgattg ccaatatgca acgttgcact aaacctggtg gttacaacct
    12961 gattgtggcg gcgatggata ccgctgatta tccatgtacc gtcggcttcc cgtttgcctt
    13021 caaagaggga gaattacgtc gatattacga aggctgggag agggtgaaat acaatgaaga
    13081 cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc gtttcgccac
    13141 gatgctggca cgtaaaaaat gacccgctaa gaataaggcg cgtgaaaatt ccccacgctg
    13201 agatgattta ctgttcttct tttcggtaag catatttttt atcgaaggga tgtgaaatta
    13261 atcacagtag tcgaagtttt ttagcagctt aacttactga aatttaacta ctgatgattg
    13321 acttagcccc tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa aatttatttt
    13381 caccaacact ttttttgcca caacacgaag cggcgctttt tgctat
//
LOCUS       DAJTVE010000112        13358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000112 DAJTVE010000000
VERSION     DAJTVE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1753
                     /gene="adeD"
                     /locus_tag="OO439_003129"
     CDS             <1..1753
                     /gene="adeD"
                     /locus_tag="OO439_003129"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6317426.1"
                     /translation="NHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEIS
                     GPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETAT
                     LPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGAS
                     FTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKEL
                     NAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCML
                     CTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGK
                     QADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASD
                     FALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACG
                     LLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSH
                     LPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQ
                     GLFDVEKFAFTTLEVTE"
     gene            complement(1798..3189)
                     /gene="uhpT"
                     /locus_tag="OO439_003130"
     CDS             complement(1798..3189)
                     /gene="uhpT"
                     /locus_tag="OO439_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6317427.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3327..4646)
                     /gene="uhpC"
                     /locus_tag="OO439_003131"
     CDS             complement(3327..4646)
                     /gene="uhpC"
                     /locus_tag="OO439_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6317428.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4656..6158)
                     /gene="uhpB"
                     /locus_tag="OO439_003132"
     CDS             complement(4656..6158)
                     /gene="uhpB"
                     /locus_tag="OO439_003132"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6317429.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6158..6748)
                     /gene="uhpA"
                     /locus_tag="OO439_003133"
     CDS             complement(6158..6748)
                     /gene="uhpA"
                     /locus_tag="OO439_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6317430.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6823..7113)
                     /gene="ilvN"
                     /locus_tag="OO439_003134"
     CDS             complement(6823..7113)
                     /gene="ilvN"
                     /locus_tag="OO439_003134"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6317431.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7117..8805)
                     /gene="ilvB"
                     /locus_tag="OO439_003135"
     CDS             complement(7117..8805)
                     /gene="ilvB"
                     /locus_tag="OO439_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6317432.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8911..9009)
                     /gene="ivbL"
                     /locus_tag="OO439_003136"
     CDS             complement(8911..9009)
                     /gene="ivbL"
                     /locus_tag="OO439_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6317433.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9574..9663
                     /gene="tisB"
                     /locus_tag="OO439_003137"
     CDS             9574..9663
                     /gene="tisB"
                     /locus_tag="OO439_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6317434.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            9943..11127
                     /gene="emrD"
                     /locus_tag="OO439_003138"
     CDS             9943..11127
                     /gene="emrD"
                     /locus_tag="OO439_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6317435.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11135..11632)
                     /locus_tag="OO439_003139"
     CDS             complement(11135..11632)
                     /locus_tag="OO439_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6317436.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11629..11991)
                     /locus_tag="OO439_003140"
     CDS             complement(11629..11991)
                     /locus_tag="OO439_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6317437.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11981..12328)
                     /locus_tag="OO439_003141"
     CDS             complement(11981..12328)
                     /locus_tag="OO439_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6317438.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12437..12886
                     /locus_tag="OO439_003142"
     CDS             12437..12886
                     /locus_tag="OO439_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317439.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13135..>13358)
                     /locus_tag="OO439_003143"
     CDS             complement(13135..>13358)
                     /locus_tag="OO439_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6317440.1"
                     /translation="TSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3145 a         3730 c         3496 g         2987 t
ORIGIN      
        1 taaccataaa tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg
       61 tggcgacgct gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgg
      121 cggagaaatt tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga
      181 atacgctgat gctccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg
      241 gtttattgat gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac
      301 cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt
      361 gatgggagaa gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca
      421 gtacttacag gtcagctctt gcgtacccgc cctggaaggc tgcgatgtta acggtgccag
      481 ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga
      541 aatgatggac taccctggcg taattagcgg gcagaatgcg ctgctcgata aactggatgc
      601 atttcgccac ctgacgctgg acggtcactg cccgggtttg ggtggtaaag aacttaacgc
      661 ctatattact gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg
      721 gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg tccgctgccc gcaatctcaa
      781 cgcgctggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga
      841 ccgtaacccg tgggagatcg cccatgaagg acacatcgat gccttaattc gccgcctgat
      901 cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg
      961 ccactttggt ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct
     1021 gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg agccgattga
     1081 tgcgcaaacc ttacaggcgg aagagtcggc gagactggca caatccgctc cgccatatgg
     1141 caacaccatt gcccgccagc cagtttccgc cagcgacttt gccctgcaat ttacgcccgg
     1201 aaaacgctat cgggtcattg acgtcatcca taacgaattg attacgcact cccactccag
     1261 cgtctacagc gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg
     1321 ttacgggcaa cggctggctc cggcttgtgg tttgcttggc ggctttggac tgaatgaagg
     1381 tgcgctggct gcgacggtca gccatgacag ccataatatt gtggtgatcg gtcgcagtgc
     1441 cgaagagatg gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt
     1501 acgtaacggc caggtccaaa gtcatctgcc gttgcccatt gccggcctga tgagcaccga
     1561 cacggcgcag tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg
     1621 tccgttaccc gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc
     1681 gctaaaacta accagtcagg ggctatttga tgtcgagaag tttgccttca ctacgctgga
     1741 agtcacggaa taataaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta
     1801 tgccactgtc aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccatcaccgc
     1861 cactatcgcc atcaggcaga tacaaccaat cgcggcgata tcaagtgcgg cgaaggtgcc
     1921 tgcccagccg gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt
     1981 ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat
     2041 cgctttttta ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac
     2101 caggaaaccc aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata
     2161 cacaccgagc gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc
     2221 gttcgccagg tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa
     2281 cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac
     2341 ggttgaccac tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag
     2401 cagccagatc actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc
     2461 ggtagattct gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt
     2521 gccgaggcca taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat
     2581 cagcgcgata atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt
     2641 tgccccgaac agcgccacgc ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca
     2701 gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact
     2761 gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa
     2821 caggctaacc gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagacag
     2881 gatcagcatg aacggcagga attgtttggt gtttttgccg tcggcgtagt aggaaaccag
     2941 cgttttaccc acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat
     3001 gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg ttaaagttct tgcgaatcag
     3061 gtacatcgtc aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca
     3121 cattttgcgc cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaagaa
     3181 agccagcatg ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag
     3241 cagcaaatca ggcctgagat agcgtccagg caaaacctgg aaaatttctt agttttgccg
     3301 gatatgaagt gaaaaggtga gatgcatcac gcttcgcgcg gtgtctgggc gttcaaaaag
     3361 ggcagtaaca gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc
     3421 cagtgccagg tatcgagtac tttcgccagc ggccaaccag caagcgacgc ccccagataa
     3481 gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc
     3541 gccgccatac cgattaacat ctgtgggcca aagacaaaaa aaccaatggt gaagaagcag
     3601 gttgcctgca tcacgtagct ggcaaatggc atcagccaca gggagccgac tgaaagcaaa
     3661 attccggcgg cgaaaatcaa attcatcggc cctcggttgc cgttaaacaa tttgtccgag
     3721 ccccaacccg ctaccagcgc accgataaat ccacccagtt caaacatcgt cactgccgta
     3781 ttcgccgtga ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg
     3841 atcgccgccc ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga
     3901 ttcagcaaca catatttggt gaggatctct ttacgcgtca gccctgcccc ttcttgttgt
     3961 tgagctatct ccagcgcgtc gtgtcgccat tcaccgaccg ccggtaaacc taacgcctgc
     4021 gggcgatcgc gcagccgcca gcagagaaaa attcccacga cgatcgccat acaaccagca
     4081 atcatcatcc cggcacgcca gccgtaatgt agtgcagccg ctgccatcac aatgggaatg
     4141 agtgcgccgc cgacgttatg cgccgtgttc cataatgccc accaaccacc gcgctcggta
     4201 cgtgaatacc aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag
     4261 gcgttcagca cccagagtac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg
     4321 ttgataatgc ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg
     4381 ctgacaatgc cggagacaaa cttcgacacg ccataggtaa tgtaaaacag ggtcgctaac
     4441 aggccgatat cgctacggct gagcacgccg ttagcaagga tttctggtac ggcggcgtta
     4501 aaacttttcc gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc
     4561 cgacgccagt agcgatagcg ggcatcaatt tcatatttat cagccattaa tggcgcatcg
     4621 gcaggcgctt tcagaaacgg caacatcatc aaaccttaga catagcgttg aggtagagaa
     4681 acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg tgccacccag cgccgttacg
     4741 cgctcgcgca ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg
     4801 ccatcgtctt caataaccag catcaaccgt tcatcctgct gccagccttg cagggtgacc
     4861 gcgctggcat cagcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc
     4921 gtcacgcgct ggttttcgct taacgctgat tcatcgattc gccattcgag atggctgaca
     4981 ataccgcgcc cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg
     5041 agatcatcca actggcgcgg acgtaaccga cccaacaaac ggcgcaccgc gtcgtaaacg
     5101 cccagcgata gttgttcgat gagttgcccg ctctgcttca cgctggcgtt atctgccgcc
     5161 agccgctgaa caatgcccgc ctgagtacga atagcagtga tggtctggcc gatatcatca
     5221 tgcagctcac gcgccacatc acggcgcacg ctctcttcgg tttccagcaa ccgttcagcc
     5281 agatgctgat tgcgcgccag ttccttttgc agcgactggt taagttcacg caaccgctgg
     5341 atgccagcgc caagcaacaa ccctgtcaga ctttgcacca gcagcgagag caataaatcc
     5401 accggatgat cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca
     5461 atcagcgccc cttgccaacc atagtgccag gccagcgcga taatcggcag caccagacag
     5521 aatggcgtaa agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca
     5581 aacagcagca agtaccagac cagatgtcgc ccgcgccagt tgattggctg agaaaccagt
     5641 gacgggccga gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc
     5701 ggggccagcg tcaggccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg
     5761 tgccaaagcc agggcagcga ctgcaacaac gccgccgccg ttaacgccgc cccctgtaac
     5821 agcaaggtgc gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac
     5881 gtcagtaaac taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc
     5941 cagtaaatca gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc
     6001 agcattagcc ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca
     6061 accagatgca ggctgatact ccacaggcaa aaccatgcgg cagagaagat aaaaaagcag
     6121 gcaataacgg taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc
     6181 gcgccagctc tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt
     6241 gtaccgtttt cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg
     6301 ccagtttttc cgccacctgg cgttcacgtt tggttagcgg gtcctgacga ccggatgcca
     6361 gtttaatggc aatatccggc gtcagataac agccacccgt ggcaaccgta tgcaccgcag
     6421 caatgagttc atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg
     6481 cctgctcaac cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg
     6541 gcagctggct tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca
     6601 cctgcacacc gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa
     6661 ctacctgcaa atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt
     6721 gatcgtctat aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc
     6781 ttaccttaac gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc
     6841 gatcttgtta aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag
     6901 cttatcgatt tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat
     6961 atggcttttg tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg
     7021 gcgggcaaaa aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc
     7081 cagaattacg ttgtcatgag ttgtgttttg catggcttat tcccccacca tttcagtatt
     7141 cgccgcacct ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat
     7201 cagcgccggg ctagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt
     7261 attcaaatca caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc
     7321 cggataggtg gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag
     7381 ccccagcgct tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt
     7441 cgccatctcc tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg
     7501 atccgggttc gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag
     7561 cccaccggag gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat
     7621 ctgatgctga cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc
     7681 gttgatcagg ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc
     7741 gcgctgcaaa tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacctgcgg
     7801 gatcaattgc gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat
     7861 tttacccagc tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg
     7921 ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc
     7981 ctcctgcaaa atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa
     8041 cggatgcgct tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc
     8101 tttctccgcc agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag
     8161 caccgggcgt ttggcagcgt taatcattgt agctacgtca cgaatgcttt cttcgctaaa
     8221 ggcgggtgca gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt
     8281 ttgtacatcc ttaggaatgt ctatccacac cgggccaggg cggcctgatt gcgcaatgcg
     8341 gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt ctgaccagat agttgtgttt
     8401 ggtgatgggg atagagatgc cgtaggtgtc gacttcctgg aaggcgtcgg tgccgatcat
     8461 cgaggcggga acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcggc
     8521 aatggcggtc accaggttag tcgcacccgg tccgctacag gccatacaga ccgccggttt
     8581 accgtcggtg cgcgccattc cctgagcgat aaagcccgcg ccctgttcat gacgggccag
     8641 aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa acaggcagga tagaaccgcc
     8701 cggaatgcct gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc
     8761 gccggtaaag cgcttacgcg tcaatgttgt gcccgaactt gccatgctcc agtccttttc
     8821 ttctgggccg actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccgacg
     8881 gggttttgga atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac
     8941 acgcacgacg accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga
     9001 agtagtcatt ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt
     9061 acaggggaga caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg
     9121 tactgaattg cagataacaa aaaaccccgc cggagcgagg tttcgtcagt cgcccgcggc
     9181 tggtaaccgc aaagcacact gtattatgtc aacacagaaa gtatacgtgt tccgcgcaga
     9241 acgcgcaatt tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgatcacagt
     9301 ttgcgttttg tccaaatatt gctgtttatt tatacagtaa acttctataa tatcactgta
     9361 cgcaatgtgt tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg
     9421 gtggtgccgt cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaaa
     9481 gtttctcctt gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca
     9541 gctggcagtg ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt
     9601 atcctcaaac tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag
     9661 taattcagat tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc
     9721 gtgcgggttg gtgtcggatg cgatgctgac gtatcttatc cgccctacca tctctcccgg
     9781 caacatttat tgccgctttt gtttacatat tctgccgcta aacaactccc cattcctggc
     9841 gtatatctgg ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg
     9901 tagtgactta ctctaattat ttttataaaa gcatccgtga taatgaaaag gcatagaaac
     9961 gtcaatttgt tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt
    10021 tatattccag ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag
    10081 agcgtaatgg gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgatt
    10141 tccgaccgtg tgggtcgccg accggtgatc ctcgtcggaa tgtccatttt tatgctggca
    10201 acgctggtcg cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag
    10261 gggatgggca ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgaga tttatatgaa
    10321 cggacacagt tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg
    10381 ctcgcaccgc taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc
    10441 tttttgttgg ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg
    10501 cgtccggtcg atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac
    10561 agcggtttta actgttattt gctgatgctg attggcggtc tggccgggat tgccgccttt
    10621 gaagcctgct ccagcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt
    10681 attttgttta ttctgccgat tccagcagcg ttttttggcg catggtttgc cggacgtccc
    10741 aataaacgct tctccacgtt aatgtggcag tcggttatct gctgcctgct ggctggcttg
    10801 ctgatgtgga tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc
    10861 gcgctgttct ttttcggtgc cgggatgctg tttcctctgg cgaccagcgg cgcgatggag
    10921 ccgttcccct tcctggcggg cacggctggc gcgctggtcg gcggtctgca aaacattggt
    10981 tccggcgtgc tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctaggg
    11041 ttgttgatga ccttaatggg attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg
    11101 atgtcgcatc aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca
    11161 tgggagaacg atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag
    11221 aataaaacgc ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg
    11281 ggcacaggcg atgccagtcg cacgcctggc agtcacagcg cggttgcggg ttttcgctgc
    11341 cacaccattt ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc
    11401 gcacccaaca ctcgaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag
    11461 cggccgtcag atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga
    11521 tgccccgctg tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta
    11581 caaccagttt atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct
    11641 ctcaataaat acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa
    11701 caccgcgagg gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt
    11761 cgtgacatcc attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc
    11821 gagaatgcca atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc
    11881 caacgccatc aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc
    11941 cggctgcagt cctgggtcgg caatgcgtct ggctttgcgg ctatccggca tacaacacca
    12001 gtcccataac gatcaccgcc acgaccatca atattaagct aatgattaat aagctgttgg
    12061 tgtagggcag gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc
    12121 catacattgc taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga
    12181 cgggcgtggc gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta
    12241 gtgcggtacg gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg
    12301 gcgcttctcc aaggcgggaa attttcattg ttgctccttt gcaatttcac taactctcta
    12361 cctttagcgg ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc
    12421 gctacaagga ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct
    12481 gttcggtgcc atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc
    12541 accaccgcca acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct
    12601 tctcgccgga ataaaaggcg aaaaaattac caccgtggag aaaaaagagg acgtagatgt
    12661 cgataaaatc ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt
    12721 tgttggcggt atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggcgg
    12781 cggaacgctg gctttccata ccttgctttt tggtatcggc atcgtgtgca gcattttgct
    12841 gatattcctt atcttttcct tcctgaccgg cggttcgctg gtttaagcag cctgaataat
    12901 gcccgccggg tgatcatcag cgggcattat cgtcagaact tctgtttttt ctcttccacc
    12961 ttcacccctt gtgtcggtag gccggtgtca tagtcacgta ccaccggcga atagccatct
    13021 tgtgggcgtg gacgaaatgc ccccatccag cgcggccgtg catctttacg ccacggacgc
    13081 aggctccatt gttgacatcc tccacgccct gaaggacgtg gccccctgct acgttcaggc
    13141 tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact gcaccatctc
    13201 tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccat ttacatcggc
    13261 gttcgctgta tatccacata cctggcatct gaatttactt tgtgacaggc gattttcttt
    13321 cgctgtatga ccacagcaca cgcaacgctg gcttgtgt
//
LOCUS       DAJTVE010000113        13312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000113 DAJTVE010000000
VERSION     DAJTVE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..410)
                     /gene="dsbG"
                     /locus_tag="OO439_003144"
     CDS             complement(<1..410)
                     /gene="dsbG"
                     /locus_tag="OO439_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6317441.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQAR"
     gene            782..1345
                     /gene="ahpC"
                     /locus_tag="OO439_003145"
     CDS             782..1345
                     /gene="ahpC"
                     /locus_tag="OO439_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6317442.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1516..3081
                     /gene="ahpF"
                     /locus_tag="OO439_003146"
     CDS             1516..3081
                     /gene="ahpF"
                     /locus_tag="OO439_003146"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6317443.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3202..3630)
                     /gene="uspG"
                     /locus_tag="OO439_003147"
     CDS             complement(3202..3630)
                     /gene="uspG"
                     /locus_tag="OO439_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6317444.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            3851..5089
                     /locus_tag="OO439_003148"
     CDS             3851..5089
                     /locus_tag="OO439_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6317445.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5320..5730)
                     /gene="rnk"
                     /locus_tag="OO439_003149"
     CDS             complement(5320..5730)
                     /gene="rnk"
                     /locus_tag="OO439_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6317446.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(5960..6766)
                     /gene="rna"
                     /locus_tag="OO439_003150"
     CDS             complement(5960..6766)
                     /gene="rna"
                     /locus_tag="OO439_003150"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6317447.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(6880..8343)
                     /gene="citT"
                     /locus_tag="OO439_003151"
     CDS             complement(6880..8343)
                     /gene="citT"
                     /locus_tag="OO439_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6317448.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8394..9272)
                     /gene="citG"
                     /locus_tag="OO439_003152"
     CDS             complement(8394..9272)
                     /gene="citG"
                     /locus_tag="OO439_003152"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6317449.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9247..9798)
                     /gene="citX"
                     /locus_tag="OO439_003153"
     CDS             complement(9247..9798)
                     /gene="citX"
                     /locus_tag="OO439_003153"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6317450.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(9802..11334)
                     /gene="citF"
                     /locus_tag="OO439_003154"
     CDS             complement(9802..11334)
                     /gene="citF"
                     /locus_tag="OO439_003154"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6317451.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11345..12253)
                     /gene="citE"
                     /locus_tag="OO439_003155"
     CDS             complement(11345..12253)
                     /gene="citE"
                     /locus_tag="OO439_003155"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6317452.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12250..12546)
                     /gene="citD"
                     /locus_tag="OO439_003156"
     CDS             complement(12250..12546)
                     /gene="citD"
                     /locus_tag="OO439_003156"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6317453.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12561..>13312)
                     /gene="citC"
                     /locus_tag="OO439_003157"
     CDS             complement(12561..>13312)
                     /gene="citC"
                     /locus_tag="OO439_003157"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6317454.1"
                     /translation="LFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKI
                     GCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPR
                     LTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEP
                     FCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAI
                     APLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3277 a         3373 c         3568 g         3094 t
ORIGIN      
        1 cgcgcctgct gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca
       61 atgaccggcg catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac
      121 atttcgcgtc cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca
      181 cctttctcgt tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg
      241 gtgacgccca tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac
      301 gttttgatga ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct
      361 gcgaaggcga ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc
      421 ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc
      481 ttagatcagg tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt
      541 tgtaagggca acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat
      601 tacacggtca cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt
      661 cacaaagttg tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca
      721 ttagccgaat cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata
      781 gatgtccttg attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga
      841 attcatcgaa atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc
      901 ggctgacttt actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga
      961 actgcagaaa ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa
     1021 agcatggcac agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc
     1081 gactggcgcc ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg
     1141 tgcgaccttc gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg
     1201 cattggccgt gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc
     1261 tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc
     1321 tctggacctg gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc
     1381 gaaatacttc ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg
     1441 caccatgatg caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa
     1501 gcccaggaga taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag
     1561 aaattgacca agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa
     1621 atcaaggaac tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat
     1681 aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg
     1741 ccacgttttg caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg
     1801 tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt
     1861 gacggtgatt ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg
     1921 gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac
     1981 ggcggcacct tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc
     2041 gtaaacggga aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt
     2101 gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta
     2161 ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc
     2221 atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc
     2281 gaaaactaca tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt
     2341 cacgttgatg aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca
     2401 gcagcagttg aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca
     2461 cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat
     2521 cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt
     2581 aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg
     2641 ggtatcgttg agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt
     2701 ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg
     2761 gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc
     2821 gatattcaca acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc
     2881 aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa
     2941 tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag
     3001 cagatcatca tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg
     3061 attcgcacca aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg
     3121 gtcaaacctg gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg
     3181 cggcataaat acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat
     3241 cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat
     3301 aactacaaca tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc
     3361 aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg
     3421 cagacgttct tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa
     3481 acggtgcagg ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc
     3541 atcctgggcg aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac
     3601 atcaactggc ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa
     3661 tcattcgcca tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa
     3721 cggtttctgt gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta
     3781 ccgcccgttt tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc
     3841 tggaggttgc atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt
     3901 tcccgatccg ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat
     3961 ctgtggctct gacctccatc tttatcgagg caaaataccc caggttaaac atggcgatat
     4021 tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca
     4081 aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg
     4141 aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa
     4201 aaaacagata ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc
     4261 tggtggacag gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc
     4321 gcctttgctt tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca
     4381 ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc
     4441 agtgggattg ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt
     4501 tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa
     4561 tttcgatgaa gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg
     4621 cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt
     4681 gctgactaac ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc
     4741 ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt
     4801 cctgtttggc gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca
     4861 cgcatggctg ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat
     4921 tgttacccac tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg
     4981 tgaagaggag tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca
     5041 gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt
     5101 ggttttcgag gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca
     5161 ggatgtaaaa tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca
     5221 aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca
     5281 tcctctgggc aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc
     5341 agcttctggc tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag
     5401 ttcccagtga atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat
     5461 aacggaaagc tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg
     5521 cacttcgcca tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg
     5581 tggcatctct tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc
     5641 gattggcaaa ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag
     5701 gtcgttaatg atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg
     5761 tcgcagataa acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac
     5821 atgatgatac tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc
     5881 tcaatgcagc gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg
     5941 tgttttgtaa atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt
     6001 tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag
     6061 ctttgatcga gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa
     6121 ctgctttcac gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc
     6181 tcactgtttt accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt
     6241 gattcaggcg taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt
     6301 gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac
     6361 ttagtttagc ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg
     6421 cgtgcgcttc tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc
     6481 gttcatcaac accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg
     6541 tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac
     6601 gatttcgatc gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc
     6661 gatcaaaatc gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa
     6721 gcagagaaac cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac
     6781 tcatacatac actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc
     6841 tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat
     6901 cggccagcca accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg
     6961 ccagtaatct tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc
     7021 atacggcgtc agacagccca tgataccgat agacagcacc agcaggatac acagttgttc
     7081 cattggtacg cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt
     7141 gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat
     7201 caccgttgcg tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc
     7261 aataaagcca gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag
     7321 cgtgttccat gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag
     7381 cattagcgaa actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac
     7441 ccacaaacct aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc
     7501 acccatggtt ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc
     7561 cggtttgtac agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg
     7621 caggaagcag aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa
     7681 ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt
     7741 actaatgacc atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg
     7801 gaatgattta aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg
     7861 cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc
     7921 caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa
     7981 cccggaaact tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt
     8041 gctggaaaaa ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc
     8101 agctaattct ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc
     8161 aataaaactg atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac
     8221 aaacacagcg aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa
     8281 catgacaccc atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga
     8341 catacgggtt ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc
     8401 tgtgctaaaa accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt
     8461 tcgatacact ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc
     8521 cctttttgca ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg
     8581 cgcgatgcaa cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat
     8641 gccagttcag gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg
     8701 atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg
     8761 tacaaccgtt gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg
     8821 ccacggcaga aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt
     8881 tgaagcaaac ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc
     8941 gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt
     9001 aatccgtgga gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa
     9061 cggggtagcc agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct
     9121 ttatgtgcac cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca
     9181 gtcagcatgg cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt
     9241 gtggttttag ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag
     9301 taaatcggtc agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa
     9361 caggcagcgg cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt
     9421 caggacatcg atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc
     9481 gagcttgagg tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga
     9541 aaccagtgca gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc
     9601 tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc
     9661 aaccacggta aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc
     9721 ttgcctttca tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag
     9781 ttcaggaagc aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc
     9841 atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc
     9901 ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc
     9961 ctgcagacgt tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac
    10021 cagaatatcg acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg
    10081 aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc
    10141 gcagtgacca ccggaagcac cacgcagtac gccgtcagag ccggtcagca cgttaacgtt
    10201 gaactgggtg tcaatttcca gcgcgctcag taccaccacg tcgagacgat caaccgatgc
    10261 gcctttcgaa ccccagttag cgtactggtt ggcgctgatt tcgatgtgat tggggttacg
    10321 ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca tccagcagtt tgcggatcag
    10381 acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg ccgagggcga agtcggcgcg
    10441 aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt accgccagcg atgcgccgcc
    10501 ggtgccggtt tgcatggaga aaccttcttt gaagtagcca gagttgacaa tcacatccgc
    10561 agcgctacgg gcaataagca gttcgcgcgg gttagtggtc atacgggtcg cgccagcgcc
    10621 gatttttgca gcatcgccaa cgcggtcaac tttgacgatc aaatcaacct gatcttgctc
    10681 aatgcttgcc ggattatgcg gataaggcag cagttcttcg gtaagcatca caacctgttt
    10741 tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg cagcaggctt taccggtgta
    10801 accgttggca ttaccgaatt catcacagga cgggacgccg aggaaagcca cgtcgatatt
    10861 cagttcgccg ctctgtacca gatgcacacg tccgccgtga gagtggattt gcaccggttc
    10921 cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg cgcaggccgg aggtataaat
    10981 gcgggtaacc acgccctggc gaatgtgttc taccagtggc gcatggcaat cactcaggga
    11041 gctggacgcc agggtcaggt ttttaaagcc catcttagcg atgacgtcca tcaccatatt
    11101 gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa accgtcatgc cgtcctgtaa
    11161 accagagcga cgaatcgctt cttcctggtt ggcgcacagt ttgcgatcgc gcgctttttc
    11221 agcctggtag gtttgctttg gtgagttctg gaaagcggca agatcgcatt cagcgcgacg
    11281 attccaggcc gctacccgtt cttgtcgttg agattgttca attttctgcg tcattttgat
    11341 tgccttattc ttcgcggatg ccggaaagtt ctgcacggga gagcaccaga cgggcacgat
    11401 cgataaccgg accgtccacc atcttgccgt tcagggaaac cacgccgagg ccttcgcgag
    11461 cggcggcttc agcggcttct acgacgcggc gggcgtgatc cacttctttc tgggtcggtg
    11521 cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat cagcgatttg ccgtcaaagc
    11581 ccagctgttt gatgtgggcg gcttcttgca ggaatccggc ttcgttgtta gcgtcggaat
    11641 agacggtatc gaacgcctga atacccgcag agcgcgcggc ctgcaaaatg gaacagcgtg
    11701 cgaacagcag ttcagttcct tccggggagc gttctgtacg caggttgcgc acatagtctt
    11761 ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg agcgatttcc actgcgcggg
    11821 taatccccag cggagattca atcgccgcca gcaggccggt gctgccgggt tcacgaccac
    11881 aggctttttc gatacgcagg atctcttttt cgatatccag aacatcctga gcggtatcgg
    11941 ttttcggcag acgcacaacg tccgcaccac cgcgaacgac ggcttccagg tcgttgacac
    12001 cccattcgga atccagcgcg ttgacacgca caatggtttc aatatcgcga tacagcggat
    12061 gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt ttcacgcaat gctacggagt
    12121 cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa ggagttgctg accatcgcgg
    12181 cattggcacc aggcacaaac aacatgctgc ggcgggtgcg agttttacgt tgttgcagcg
    12241 aagcggaaat cattggcaat cctcccatgg cagagccggg ataccgctgg cgcgtgccag
    12301 cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg cctttgtcat cgacattcag
    12361 ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga atggtggtgc gaattgcatc
    12421 gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg atatcctgcg tatcgagtgg
    12481 ggcgatgcgt atcatcacat ccccagactc aagggtgcct gcaacggcgg gctggtttat
    12541 tttcattttt cacctgtttc tcatgcgggg gtcttttgac gagctgccgc gtcctggcgg
    12601 gagtgctcaa gcaggttctg caaataatgc agcgtgactg cagggaccag cggcgcgata
    12661 gccgtgagat cgtttttcgc cagcagttga cgtacccggg aagcggatat cggcatctcc
    12721 tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg gtgcggagat agtcggcgtt
    12781 tccagccagt agcgcatatc ctggttgtac tgggcggtaa cgcgacaaaa gggttcagta
    12841 ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt actgacggaa aatcttcaga
    12901 tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt taatgaagta gcaagggaac
    12961 gtagcgcggg agatgatgta ttcggagccg cgatgcacag tcaggcgtgg aatatcggcg
    13021 gtgcctttta acaccagatc caaccggtct tcatagggga agcgtgaaga atcttctttg
    13081 actaaaaaca gatgcaacca gtcgcactgt gccgcagctt gttgaatcag ataacggtga
    13141 ccattcgtga agggattggc gttcatcaca atgcagccaa tcttgttccc tggatgacga
    13201 aattttttca gcgattcggc atagcgtttc agccgcgtgg cgctgttttc catcagcacc
    13261 atcacgccgg gtacgctggt cagcgtggaa aaaccgcact ggcggaacag cg
//
LOCUS       DAJTVE010000114        13083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000114 DAJTVE010000000
VERSION     DAJTVE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO439_003158"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO439_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6317455.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO439_003159"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO439_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6317456.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO439_003160"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO439_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6317457.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO439_003161"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO439_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6317458.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO439_003162"
     CDS             complement(4036..5568)
                     /locus_tag="OO439_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6317459.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PYQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO439_003163"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO439_003163"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6317460.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO439_003164"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO439_003164"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6317461.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OO439_003165"
     CDS             complement(8663..10399)
                     /locus_tag="OO439_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6317462.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO439_003166"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO439_003166"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6317463.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OO439_003167"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OO439_003167"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6317464.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OO439_003168"
     CDS             complement(12121..12855)
                     /locus_tag="OO439_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6317465.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
BASE COUNT         3343 a         3330 c         3425 g         2985 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatc tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtacgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttg
//
LOCUS       DAJTVE010000115        13000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000115 DAJTVE010000000
VERSION     DAJTVE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /locus_tag="OO439_003169"
     CDS             <1..191
                     /locus_tag="OO439_003169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317466.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            98..1393
                     /locus_tag="OO439_003170"
     CDS             98..1393
                     /locus_tag="OO439_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6317467.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO439_003171"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO439_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6317468.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="OO439_003172"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="OO439_003172"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6317469.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="OO439_003173"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="OO439_003173"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6317470.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="OO439_003174"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="OO439_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6317471.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="OO439_003175"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="OO439_003175"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6317472.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="OO439_003176"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="OO439_003176"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6317473.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="OO439_003177"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="OO439_003177"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6317474.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3578 g         3154 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagggt
//
LOCUS       DAJTVE010000116        12986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000116 DAJTVE010000000
VERSION     DAJTVE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(613..1704)
                     /gene="ychF"
                     /locus_tag="OO439_003178"
     CDS             complement(613..1704)
                     /gene="ychF"
                     /locus_tag="OO439_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6317475.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1821..2405)
                     /gene="pth"
                     /locus_tag="OO439_003179"
     CDS             complement(1821..2405)
                     /gene="pth"
                     /locus_tag="OO439_003179"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6317476.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2682..2960
                     /gene="ychH"
                     /locus_tag="OO439_003180"
     CDS             2682..2960
                     /gene="ychH"
                     /locus_tag="OO439_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6317477.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3015..4694)
                     /gene="dauA"
                     /locus_tag="OO439_003181"
     CDS             complement(3015..4694)
                     /gene="dauA"
                     /locus_tag="OO439_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6317478.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4818..5765)
                     /gene="prs"
                     /locus_tag="OO439_003182"
     CDS             complement(4818..5765)
                     /gene="prs"
                     /locus_tag="OO439_003182"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6317479.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5916..6767)
                     /gene="ispE"
                     /locus_tag="OO439_003183"
     CDS             complement(5916..6767)
                     /gene="ispE"
                     /locus_tag="OO439_003183"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6317480.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6767..7390)
                     /gene="lolB"
                     /locus_tag="OO439_003184"
     CDS             complement(6767..7390)
                     /gene="lolB"
                     /locus_tag="OO439_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6317481.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7604..8860
                     /gene="hemA"
                     /locus_tag="OO439_003185"
     CDS             7604..8860
                     /gene="hemA"
                     /locus_tag="OO439_003185"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6317482.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8902..9984
                     /gene="prfA"
                     /locus_tag="OO439_003186"
     CDS             8902..9984
                     /gene="prfA"
                     /locus_tag="OO439_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6317483.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9984..10817
                     /gene="prmC"
                     /locus_tag="OO439_003187"
     CDS             9984..10817
                     /gene="prmC"
                     /locus_tag="OO439_003187"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6317484.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10814..11206
                     /locus_tag="OO439_003188"
     CDS             10814..11206
                     /locus_tag="OO439_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6317485.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11210..12018
                     /locus_tag="OO439_003189"
                     /pseudo
     CDS             11210..12018
                     /locus_tag="OO439_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12054..12908
                     /gene="kdsA"
                     /locus_tag="OO439_003190"
     CDS             12054..12908
                     /gene="kdsA"
                     /locus_tag="OO439_003190"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6317486.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3266 a         3235 c         3349 g         3136 t
ORIGIN      
        1 tacttattcc aattcgttac tataaatatg gcaaaaatat tacaacaaac agcggttaaa
       61 aaatttggtg aagatttttt gtaataatcc taattagtta tgggaatttc gcttgatgta
      121 tctgcataac aaaaacaata tgcgccattt ttgcattact aataagaaaa agcaaaaaac
      181 atcgatattc atttttggtc aatgggtttt ctggcctatc gttgttattg tgctactggt
      241 tatcattttc atcttgccag gatcgcacgt aatcacgtta ttcggggact ttatctgcgg
      301 gatattaccc ttcaggtaag tccttttctc gccagcatat tggagcgtga tcaatattga
      361 tcagctgtga agggggtgat gtgtagttga acaaaaactt acgttatatc actactaatg
      421 agatatccgt catctgacac tgatgtcttt aattattctc gtggttcact gtaggcaata
      481 agcacaaaag tgtaggatgt tacaagaatg attaggactc ggtgaaatga aaaatccacg
      541 caattgcgtg gattttatat acttttgcgc tcttcatgag atttagcgaa acctcatgag
      601 acaataaatt aattagacgt tgaaaaggaa gttcatcaca tcgccatctt taacgatgta
      661 atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt
      721 gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa aatcagtatg
      781 gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg cacgcacttc
      841 tttcacccca gcggtgaagt aagtttgcag gttcagcagc ttataaccga cacggatcac
      901 acggttcagg cccggttctt ccagcccaag ctcctgcata aactcgtcac gttcttcgtc
      961 gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa cagaaccttc
     1021 tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt
     1081 gacgttggcg atgtacattg ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc
     1141 tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcgttttcca gctggggcag
     1201 gcatttttct aggaccgcca gctcagcttt cgcgtcttta tcgccacctt tggctttctt
     1261 ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt
     1321 gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga tgttgtcatt
     1381 ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg tcaggaactg
     1441 gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc
     1501 catggtcgtg ggaagcgtac gttgcggttt tacgatttca gccagttgat ccaggcgagg
     1561 gtcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc
     1621 ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc
     1681 gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat aatcaacctg
     1741 ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg gcactcggca
     1801 aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg
     1861 gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcgat ggcttcatca
     1921 attaacttct gttcactaac aggcggtttg cctaacacaa aaccgacaac tttattttta
     1981 tcgccaggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt acccaattta
     2041 ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaattt aaatttggcg
     2101 acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg
     2161 aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt agtcgggact
     2221 aacaggcgga catcttcgcc tccaagagtg actcgcgaag tataaccaaa gaatttagcc
     2281 tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca ggcaccagca
     2341 ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat caatttaatc
     2401 gtcacgtttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc
     2461 gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt
     2521 agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt
     2581 gtgatcacgc ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg
     2641 ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt
     2701 cgttactcgg taacgtgctc atggggttgg ggctggtggt aatggtggtc ggcgtggggt
     2761 attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag
     2821 tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg
     2881 aacaggtgtg cgaccgttac tggtgggttc gtcactatga caaacgttgc cgccgtagcg
     2941 ataatcgccg tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact
     3001 ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg
     3061 cgtcccggga tcggttgaat gcctgcgcga gccatagtgc gcagtggctg gaattccacg
     3121 ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca
     3181 agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct
     3241 tcaagacgtg actccaggtc cgtgaacaag ccttcagcag cagcaaaaaa cagcgggcca
     3301 ataacgcgca gtaccagaac atcgtctgga acatctacga ctaccggtgc caggcgagtc
     3361 atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca
     3421 ataaccatat caaacagcac ggtcagcgac atgcacagca gcatgacaat gatgtcatct
     3481 ttcggtgcat gacgcagcaa gtcgaccact ttatgcgctt cactcatgtt ccacgccacc
     3541 atcaacagca gggctgccat agcggaaagc ggcagccagg agagcagcgg tgccagtacc
     3601 agcagggcaa gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg
     3661 gcacggacgt tagcggcaga acgcgcgatg gcagctgtag cggtaatacc accaaagaac
     3721 ggagcgataa tattccccag tccctgtcca accagttcgc tgttcgcctt gtgtttcgtc
     3781 ccggtcatac catccagtac cacggcgcag agcagagatt cgattgcgcc gagcattgcc
     3841 attgagaatg ccgcaggcag cagtgtgcga atagaatccc aggttagcgt gaattctgaa
     3901 ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc gttaccctga
     3961 gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc
     4021 aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa
     4081 cgaatgccca gacgcggcca aaaaacaaga atacctagcg tcacaatgcc aatggcagca
     4141 tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt
     4201 tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg
     4261 atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcaggcga
     4321 ccaaagcgtg ccagacccat cagaatcaaa aagatccccg acagcaaggt cgcaaccagc
     4381 agtcctgcca gtccaaactg ttgcgacacg ggatagagaa ttaccacaaa tgccgcagtc
     4441 ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc cgcaacagct
     4501 gcggtatata aaccgtactg gggtgccaca ccactaccaa tagccaacgc catcgccagc
     4561 gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca
     4621 gtatattttt ctttccagca agcgtcgatc agagcgcgga aaggcatcac atgtgaggaa
     4681 aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag
     4741 gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg
     4801 tctttgagcg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
     4861 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
     4921 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
     4981 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
     5041 ttagcaccac gttctttcag agcttcagca gctttacaca gcgtaccgcc agtgtcgatc
     5101 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
     5161 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
     5221 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
     5281 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
     5341 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
     5401 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
     5461 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
     5521 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
     5581 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc gttgatttgt
     5641 acactgactt cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
     5701 cggttggcaa tacgttgtgc tagttccggg gtggcgttac cagcaaaaag cttcatatca
     5761 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
     5821 gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca
     5881 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
     5941 gaaagattaa cacctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
     6001 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
     6061 cgcgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
     6121 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
     6181 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ctggtgtcgg aatacttaca
     6241 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
     6301 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
     6361 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
     6421 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
     6481 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
     6541 ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
     6601 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
     6661 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
     6721 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
     6781 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
     6841 tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
     6901 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
     6961 ccggtaaacc taaaatccat tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
     7021 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
     7081 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
     7141 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
     7201 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
     7261 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
     7321 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
     7381 gctggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
     7441 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
     7501 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
     7561 aagcagacta accatatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
     7621 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
     7681 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
     7741 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
     7801 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
     7861 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
     7921 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgc
     7981 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
     8041 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
     8101 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
     8161 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
     8221 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
     8281 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
     8341 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
     8401 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
     8461 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
     8521 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
     8581 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
     8641 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
     8701 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
     8761 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
     8821 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
     8881 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
     8941 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
     9001 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
     9061 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga tgatcctgaa
     9121 atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa
     9181 cagcaattac aggttctgtt actgccaaaa gatcctgatg acgaacgtaa cgccttcctc
     9241 gaagtccgag ccggaaccgg cggcgacgaa gcggcgctgt tcgcgggcga tctgttccgt
     9301 atgtacagcc gttatgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
     9361 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
     9421 cgtctgaaat ttgaatccgg cggtcatcgc gtgcagcgtg ttcctgctac ggaatcgcag
     9481 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
     9541 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
     9601 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
     9661 gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagctaaagc actttctgta
     9721 cttggtgctc gtatccacgc tgcagaaatg gcaaaacggc aacaggccga agcgtctacc
     9781 cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta caacttcccg
     9841 caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgtctgga tgaagtgatg
     9901 gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg
     9961 gcggcgttgt ccgagcagga ataatggaat atcaatactg gttacgtgaa gcaataagcc
    10021 aacttcaggc gagcgaaagc ccacgccgtg acgctgaaat cttattggag tatgtcactg
    10081 gcagggggcg gacttttatt ctggcctttg gtgaaacgca gctgactgac gaacaatgtc
    10141 agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg
    10201 gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc
    10261 cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta
    10321 ttctcgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg
    10381 actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg
    10441 cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag
    10501 ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac
    10561 atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg
    10621 gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct
    10681 ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg
    10741 cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg
    10801 gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc
    10861 gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc
    10921 atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg
    10981 cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac
    11041 tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg
    11101 tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta
    11161 catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt
    11221 agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc
    11281 aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct
    11341 ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc
    11401 gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc
    11461 attatggctg gaccaggtgt taaagaatcg acaggcagtg cggtatcatt aggtgcggtt
    11521 ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt ccctacgcag
    11581 ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc ttttaacggt
    11641 gaatcgttaa gcgaacatat gctggacgta tggttaaagg gaaatatcag cccgtcggcg
    11701 gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg caaattgttg
    11761 gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt acgcaccagc
    11821 gaagctttat tacaattcaa cccggaagat ccctatgaaa ttcgcgatcg cgggttgatt
    11881 tatgcgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt cgttgaacag
    11941 tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa
    12001 catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat cctatgaaac
    12061 aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc gtactgtttg
    12121 gtggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag cactacgtaa
    12181 ccgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac aaagccaacc
    12241 gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa atcttccagg
    12301 agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac
    12361 agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct cgccagactg
    12421 acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg
    12481 tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc aacgaaaaag
    12541 tgattctttg cgatcgcggt gctaacttcg gctatgacaa cctggttgtc gatatgctgg
    12601 gcttcagcat tatgaagaaa gtgtctggta actcgccggt gattttcgac gtgacccacg
    12661 cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct caggtggctg
    12721 agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa gcgcatccgg
    12781 atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt
    12841 tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa ctggatacca
    12901 gcaagtaatc ttttttgctt gaaaaataaa gccttagcgt tctgcgttaa gacttttttc
    12961 atgggtgcca gatacaaaaa aggccg
//
LOCUS       DAJTVE010000117        12487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000117 DAJTVE010000000
VERSION     DAJTVE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OO439_003191"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OO439_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6317487.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OO439_003192"
     CDS             433..621
                     /locus_tag="OO439_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6317488.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OO439_003193"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OO439_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6317489.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OO439_003194"
     CDS             816..989
                     /locus_tag="OO439_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6317490.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OO439_003195"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OO439_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO439_003196"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO439_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6317491.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OO439_003197"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OO439_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6317492.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO439_003198"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO439_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6317493.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OO439_003199"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OO439_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6317494.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OO439_003200"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OO439_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO439_003201"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO439_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6317495.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO439_003202"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO439_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6317496.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3203 c         3269 g         3004 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggtat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DAJTVE010000118        12450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000118 DAJTVE010000000
VERSION     DAJTVE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..194
                     /gene="bdm"
                     /locus_tag="OO439_003203"
     CDS             <1..194
                     /gene="bdm"
                     /locus_tag="OO439_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6317497.1"
                     /translation="NSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDIL
                     SDIYQQTINYVVSGQHPTL"
     gene            296..433
                     /gene="sra"
                     /locus_tag="OO439_003204"
     CDS             296..433
                     /gene="sra"
                     /locus_tag="OO439_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6317498.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            590..2287
                     /gene="maeA"
                     /locus_tag="OO439_003205"
     CDS             590..2287
                     /gene="maeA"
                     /locus_tag="OO439_003205"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6317499.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2421..3423
                     /gene="adhP"
                     /locus_tag="OO439_003206"
                     /pseudo
     CDS             2421..3423
                     /gene="adhP"
                     /locus_tag="OO439_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3569..3853
                     /locus_tag="OO439_003207"
     CDS             3569..3853
                     /locus_tag="OO439_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6317500.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4081..4734)
                     /gene="fdnI"
                     /locus_tag="OO439_003208"
     CDS             complement(4081..4734)
                     /gene="fdnI"
                     /locus_tag="OO439_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6317501.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4727..5611)
                     /gene="fdnH"
                     /locus_tag="OO439_003209"
     CDS             complement(4727..5611)
                     /gene="fdnH"
                     /locus_tag="OO439_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6317502.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5624..8671)
                     /gene="fdnG"
                     /locus_tag="OO439_003210"
     CDS             complement(5624..8671)
                     /gene="fdnG"
                     /locus_tag="OO439_003210"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8084..8086),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6317503.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8903..9784
                     /gene="yddG"
                     /locus_tag="OO439_003211"
     CDS             8903..9784
                     /gene="yddG"
                     /locus_tag="OO439_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6317504.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10043..11143
                     /gene="ompC"
                     /locus_tag="OO439_003212"
     CDS             10043..11143
                     /gene="ompC"
                     /locus_tag="OO439_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6317505.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11307..11789
                     /locus_tag="OO439_003213"
                     /pseudo
     CDS             11307..11789
                     /locus_tag="OO439_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11904..>12450
                     /locus_tag="OO439_003214"
     CDS             11904..>12450
                     /locus_tag="OO439_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6317506.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         3232 a         3104 c         2967 g         3147 t
ORIGIN      
        1 aaaattcaac agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc
       61 aaggtgttgt cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg
      121 ataacgacat cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc
      181 accctacgct ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt
      241 ttcttcgcta cggcgatgtt gtccttaaaa ctagcaacag gattgaggag ttaaaatgaa
      301 atcgaaccgt caggcacgtc atattcttgg actggaccat aaaatttcta accagcgcaa
      361 aatagttacc gaaggtgaca aatccagcgt ggtaaataac ccaaccggca gaaaacgccc
      421 cgctgaaaag taattcataa ccatcagtcc tcaatgacga ttaaacacca ttgcctgcgc
      481 aatggtgttt ttgtttttat ctgctttata cttggggccg acgccctggc ggtaaagcaa
      541 agacgataaa agcgtgccag ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa
      601 aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc cctgtactgc tggaatttcc
      661 gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc cgtaacttca acctgctggg
      721 gttactgccg gaagtggtcg aaaccatcga agaacaagcg gaacgagcat ggatccagta
      781 tcagggattc aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc aggacaccaa
      841 cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc ctgttattta
      901 taccccaacc gtcggcgcag cctgtgagcg tttttctgag atctaccgcc gttcacgcgg
      961 cgtgtttatc tcttaccaga accgccacaa tatggacgat attctgcaaa acgtaccgaa
     1021 ccataatatc aaagtgattg tggtgactga cggtgaacgt attctggggc ttggtgacca
     1081 gggcatcggc gggatgggca ttccgatcgg taaactgtcg ctctataccg cctgtggcgg
     1141 catcagcccg gcgtataccc ttccggtggt gctggatgtc ggaacgaaca accaacaact
     1201 gcttaacgat ccgctgtata tgggctggcg taatccgcgt atcactgacg acgaatacta
     1261 tgaattcgtt gatgaattta tccaggctgt gaaacaacgc tggccagacg tgctgttgca
     1321 gtttgaagac tttgctcaaa aaaatgcgat gccgttactt aaccgctatc gcaatgaaat
     1381 ttgttctttt aacgatgaca ttcagggcac tgcggcggta acagtcggca cactgatcgc
     1441 agcaagccga gcggcaggtg gtcagttaag cgagaaaaaa atcgtcttcc ttggcgcagg
     1501 ttcagcggga tgcggcattg ccgaaatgat catcgcccag acccagcgcg aaggattaag
     1561 cgaggaagcg gcgcggcaga aagtctttat ggtcgatcgc tttggcctgc tgacggacaa
     1621 gatgccgaac ctgctgcctt tccagaccaa actggtgcag aaacgcgaaa acctcagtga
     1681 ctgggatacc gacagcgatg tgctgtcact gctggatgtg gtgcgcaatg taaaaccaga
     1741 tattctgatt ggcgtctcag gacagaccgg gctgtttacg gaagagatca tccgtgagat
     1801 gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt cacgcgtgga
     1861 agccacaccg caggacatta tcgcctggac cgaaggtaac gcgctggtcg ccacgggcag
     1921 cccgtttaat ccagtggtat ggaaagataa aatctaccct atcgcccagt gtaacaacgc
     1981 ctttattttc ccgggcatcg gcctgggggt tattgcttcc ggcgcgtcac gtatcaccga
     2041 tgagatgctg atgtcggcaa gtgaaacgct tgcacagtat tcgccgctgg tgctgaacgg
     2101 cgaaggtctg gtactaccgg aactaaaaga tattcagaaa gtctcccgtg caattgcgtt
     2161 tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg aaaacctctg ccgaagcttt
     2221 gcaacaagcc attgacgata atttctggca agccgaatac cgcgactacc gccgtacctc
     2281 catctaagcc tgcgcccggt agtgaaggct accgggcaat tttcctctcc ctttttcaga
     2341 tctcatccat actgggtagt ggcgaataaa tctcatttgc ctcacctgct atgcagaaca
     2401 tcatccgaaa aggaggaact atgaaggctg cagttgttac gaaggatcat catgttgacg
     2461 ttacggataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa atggagtgtt
     2521 gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac aaaaccgacg
     2581 taattctggg ccatgaaggc atcggtgtgg tggcagaagt gggtccaggt gtcacctcat
     2641 taaaaccagg cgatcgtgcc agcgtggcgt ggttctacga aggatgcggt cattgcgaat
     2701 actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga tacagcgttg
     2761 atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa aagtgccaga tggtctggac
     2821 tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca cctacaaagc cgttaagctg
     2881 tcaaaaattc gtcccgggca gtggattgct atctacggtc ttggcggtct gggtaacctc
     2941 gccctgcaat acgcaaagaa tgtctttaac gccaaagtga tcgccattga tgtcaatgat
     3001 gagcagttaa aactggcaac cgaaatgggt gcagatttag cgattaactc acgcaccgaa
     3061 gacgccgcca aaattgtgca ggagaaaacc ggtggcgctc actctgcggt ggtaacagca
     3121 gtagctaaag ctgcgtttaa ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg
     3181 gctgtcggtc tgccgccgga gtctatgagc ctggatatcc cacgtcttgt gctggatggc
     3241 attgaagtgg tcggttcgct ggtcggcacg cgccaggatc taaccgaagc cttccagttt
     3301 gccgccgaag gcaaagtggt gccgaaagtc gccctgcgtc cgttagcgga catcaacacc
     3361 atctttaccg agatggaaga aggcaaaatc cgtggccgta tggtgattga tttccgtcac
     3421 taagaggtct ttgctgcgac tcccatgttc gggtcgcagc atcgcacact ctccaacatg
     3481 aaatggctaa atggattatt gttgcgtagg aaggaacaat aatgatagtg ttcccagaga
     3541 aggaacgcta ctgagagtaa agacactaat gaaaatggcc aatcatcccc gcccggggga
     3601 cattattcag gaatcactgg acgaacttaa tgtcagcctg cgcgagtttg ccagagcaat
     3661 ggaaattgcg ccctcaacgg caagccgatt gctgaccgga aaagcagctt tgacgccaga
     3721 aatggcaatt aaactttccg tggtgatcgg cagttcgccg caaatgtggc tgaatctgca
     3781 aaacgcctgg agtctggcag aggcagaaaa aacggtggat gtgtcgcgat tacgccgtct
     3841 ggttacgcaa taaaaaatgg caccttataa agcgccattt gaaactgatg acaaacgcaa
     3901 cattgcctga tgcgctacgc ttatcaggcc tacacgttca cttcaatcta ttgtatttga
     3961 agggttttca tgccggataa ggcattcacg acgcatccgg gatgaacaaa gcccacgttg
     4021 tctacaatat gaaaatggcg ccttttacag cgccattttc agcttaaagt tcaaaagaga
     4081 ttatatccct tcttcactct cttttttcgc ttctgccttc tcgatttcac gataccagcg
     4141 cggatggtgt ttcttcgccc agcgacggct caccttccct tcgatcatcc ctttaatcga
     4201 ccctttcacc cagaatgcca tatacatatg gatcaggatg gcgtggatca ggatgatacc
     4261 cgcagccgcg tggatcagca ggctgtagcg aacaacctgc atcgggaagt actgcgcaaa
     4321 gtacggacgc cagataatca ccccggtcac cagcagcacg aaaatcatgc tcatgatcga
     4381 ccagaacatc atcttttgcc cggcgttgta cttaccaaca tccgccactt tgtgctcatt
     4441 gccttttaat acttcgacaa tgttcaacag ccacggaata tctttcttat ccgggatgtt
     4501 gtgatgcaca aaacgcacaa acataaacat cagtgcgacg aaaatcgcaa tgccgaagaa
     4561 cgggtgcaaa atgcgtccca tctgcggcgt accgaaggtt tgcgtcagcc attgcagcgt
     4621 cgggaagaag aacgaaatcc cggacagcgc caccaggaag aagcaaatca ccacggtcca
     4681 gtgacaggcg cgatcaataa atttggtgcg cacaatcatt ttcgacttac tcatgatgat
     4741 cctcctcgtc atcgtccact tccttattcg ggccaatacc gatgtagtgg aaaatcaacc
     4801 cggcaaaagt ggcaataaag ccagccgctg ccagcggttt caacgcgcct ttccacaggc
     4861 ttaccgaggt gtcgatcttc ggatctttcg gcagaccgta atacagctcc ggctgatcgg
     4921 cgtgatgcag cacgtacata acgtgcgtac caccgacccc tttcgggttg tagacgccag
     4981 catgttcgta accacgcgct ttcagtttcg ccacgcgctc ttccgccagc tccagcatct
     5041 ccttcttggt gccgaagtgg atagccccgg tcggacaggt tttcacacaa gccggttcct
     5101 ggccgacgct gacgcgatcg acgcagagcg tgcatttata tacccggtta tcctctttgt
     5161 tgaggcgcgg aatattaaac ggacacccgg caatgcagta accacagccg atgcagtttt
     5221 ccgactggaa atcgacaatc ccgttagcgt actgaatgat tgcaccagca gacgggcacg
     5281 ccttcaggca gccgggatct tcacagtgca tacagccgtc tttacggatc agccactcca
     5341 gcttgccgtt ctgttcggtt tcgctaaagc gcatcaccgt ccaggacttg gcgctcagat
     5401 cggcggggtt atcgtaaacc ccgacgcagt gccccacttc atcacggatg tcgttccact
     5461 ccgaacacgc cacctgacag gctttacagc cgatacaggt ggaaacgtcg ataagttttg
     5521 cgacttctgc tttgtaatca cgcacctgag aaggcggcgt gatggagtta gttgcggacc
     5581 ttttgataat atcctgcgtt tccatagcca tctgttcgcc cccttacgcc ttctcgatgt
     5641 tgactaagaa cgctttatat tccggcgttt gcgagtttgc atcaccgaca ttcggcgtca
     5701 gagtgttagc gatataacct ttacgcgcga caccctcaaa gccccagtgg attggaatac
     5761 ccaccgtttc aacctgctga ccgtttacat tcagcggttt cagacggcgc gttaccaccg
     5821 ccaccgcgcg gataaagcca cgcttgcttg agacagtgac acgatcgcca ttattaatgc
     5881 ctttcgccgc cgccagcgtt tcgctgattt ccacaaactg ttccggctga gcaattgcgt
     5941 tgagcaatgc gtgcttggtc caggtgtgga agtgctcggt cagacgatag gtcgtaccca
     6001 catacgggaa ctgctctttt ttacccatcc gcagcgcgtc ttgttcatac agacgaacaa
     6061 ccgggttaga caccacgttc ggatgcagcg ggttagtgcc cagcggcgtt tcaatcggct
     6121 cgtagtgttc cgggaacgga ccttccgcca ttttgttgat ggcaaacagg cgtcccatcc
     6181 cttccggctg catgataaac ggcccggttg gcgtacccgg tgcggcattg ccgaagtccg
     6241 gaatatcgtt acccgtccac ttgctgccgt tccactggat cagcatccgt ttcggatccc
     6301 acggtttacc attgatatcc gccgaagcac ggttgtagag cacgcgacgg ttgagcggcc
     6361 acgcccaggc ccatcccagc gtattcccca gaccggatgg gtcggagtta tcgcggttag
     6421 ccatctggtt gccctgctct gtccagctac cggtgtagat ccagcaagaa gatgcggttg
     6481 taccgtcatc acgcagatgc gcaaagctac tcagcaactg acctttcttc gcaatcagca
     6541 cgccattagc gtcatagaga tcttccagcg catagccgtt attctctttc gccacttcgt
     6601 cagattgcgg ttcgtgcggc tgcttgtagt tccagctcat cttcatcagc ggttctacgc
     6661 ctttaccacc ttcggcctgg tacagctcgc gcagatgatg gtagataccc gccagaattt
     6721 cgccgtcgtt acgcgcttcg cccggcgcgt cctgaccttt ccagtgccac tgcaaccagc
     6781 gaccggagtt agcgatagaa ccatcttctt cggcaaagca ggtcgaaggc agacggaata
     6841 cttcagtctg aatagacgcc ggatcgacat cgttcgactc gccgtggttc tgccagaagg
     6901 tagaggtttc agtcaccagc ggatcgataa ccaccatgta cttcagcttg ctcaggcagc
     6961 tcaccacttt gtttttgtcc gggaaggacg caaccgggtt aaagccctgg cagaagtaac
     7021 cggtgacttt gccttcgtcc atcatgttga aatacttgat gacgtcgtag gtctggtccc
     7081 acttcggcag ccagtcatag ccccagttgt tctctttctg cgcggcatcg ccatagaaag
     7141 atttcatcag gctaacgaag aacttcggat agttgctcca gtagttcacc tgatcagcca
     7201 gcgtcgcttt cggcgtgttc gcttccagat acgactgcaa atcaacctgt ttttctgacg
     7261 gaagcgtcag ataacctggc aggctggtag agagcagacc taagtcagtc agcccctgaa
     7321 tgttggagtg accacgcaat gcgttcacgc cgccaccggc catacccatg ttaccgagca
     7381 gcaactggat catcgccata gtacggatgt tctgcgcacc cacggtgtgc tgcgtccagc
     7441 ccagcgcgta caggaaggtg gttgtgcgat ccggtgcgct ggtggaggcc agcacttcac
     7501 acactttcag gaagtcggct tttggcgtac cgcagatgtt ttcaacgacg tccggcgtgt
     7561 agcgggaaac gtgcgctttc agcaggttcc acacacagcg cggatgagtc agtgtttcat
     7621 cgcgttttgc atagccgttt tcatcgaact gatagttcca ggacgactta tcatactggc
     7681 gtttttcagc gtcgtagccg ctgaacaggc cgtcttcgaa agcaaaatca tcacgcacca
     7741 gcaggctggc gttggtgtaa tgtttaacgt attcggcgtt gattttgttg ttttcgatca
     7801 ggtagcgcaa aacgccagac aggaacgtaa tgtccgtacc ggaacgaata ggtgcgtaaa
     7861 tatccgccac agaagcggta cgcgtaaaac gaggatcgac aacgatcaag gttgcatcgt
     7921 tgttgttttt cgcttccatc gcccagcgga aaccgacggg atgcgcttca gcagcgttac
     7981 cgcccattac cattacgacg ttagcgtttt tgatatccac ccagtggttg gtcatcgcac
     8041 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagacg cgcgcctggt
     8101 tgtctaccgc cagcatcccg agggagcggg caaatttctg ggtcagcatc ccggtttcgt
     8161 tgctggcacc ggaggcacac agcataccgg tagaaagcca acggtttacc gttacgccct
     8221 gctcgttctt ttcaataaag ttagcgtcac ggtcagcttt catcagcttc gcaatacgtg
     8281 agaatgcttc ttcccagcta atgcgctgcc atttgtcaga acctggcgca cgatattccg
     8341 ggtagcgcag acggttttcg ctgttgacgt aatccagcaa accggcccct tttgggcaca
     8401 gcgcaccacg gcttaccgga tgatccgggt caccttcaat gtgataaatc gcttctctgg
     8461 cgtttttcgc gccatcaccc aggctataca tcaatagccc gcaacctacg gaacagtatg
     8521 tgcaggtgtt acggatctct ttagcgcgta ataatttgta gtttcgcgcc tgagccagtg
     8581 cttgcttcgg ggcaaagccc aatgccgcta ccgttgttcc agccataccg cccgcgcaga
     8641 ttttaaaaaa ttgtctgcga ctgacgtcca ttgctttcct cttttttcag gggtattact
     8701 gcgcgaggaa actaacagta aaattctgaa tctttttgcg cgaaatcaaa aagaacacgt
     8761 gtatgccaca aaataatccg cctgcgcaaa ttaacctacc tcaatagcgg tagaaaaacg
     8821 caccactgcc tgacaggcca gttaaaaaaa tgctataaaa ttcagcttaa tttttaacgg
     8881 caagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag ggctaatagc
     8941 gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagcgagg ggctcggtcc
     9001 ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct tcacggttgg
     9061 atttccgcgt attcggcaga ttccgaaagg ctatttactc gccgggagtc tgttattcgt
     9121 cagctatgaa atctgtctgg cgctttcctt agggtatgcg gcgacccgtc atcaggcgat
     9181 tgaagtgggt atggtgaact atctgtggcc cagcctgaca attctctttg ccattctgtt
     9241 taatggtcag aaaaccaact ggctgattgt accaggatta ttattagccc tggtcggcgt
     9301 ctgctgggtg ttaggcggtg agaatgggtt acattatgat gaaatcatca ataatatcac
     9361 caccagccca ttgagttatt tcctggcgtt tattggtgcg tttatctggg cagcctattg
     9421 cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg tcctgctaac
     9481 gggagcatgt ctgtggattt actattttct cacgccacaa ccagaaatgg tatttagcac
     9541 gcccgtcatg attaaactca tctctgcggc atttacctta ggatttgctt atgctgcatg
     9601 gaatgtcggt atattgcatg gcaatgtcac cataatggcg gtaggctcgt attttacgcc
     9661 tgtactttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt tctcgttctg
     9721 gcaaggcgcg ctaatggtct gcggcggttc cctgctctgc tggctggcga cacgtcgtgg
     9781 ttaaaataat tgccgggtct acggcccggc agttaatcag ataaattccg acaatattaa
     9841 aaaataaatt tcaaatacca gtaattcacc ttatatctca tttcgacttc gatcacatat
     9901 cgataaataa tattatttca tctatttttc tcattgtttt taatcaccat tctattttaa
     9961 aaacagtatg tcatttataa atgacgccgt ttaaaattcg tttagaaaat acgtttaacg
    10021 ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt gcggtggttg taactggttt
    10081 gttagctgcg aacgtagcac acgctgccga agtctataac aaggatggta ataaactcga
    10141 cctttatggc aaggttaccg ctctacgtta ttttactgat gataagcgtg acgatggtga
    10201 taaaacttat gcccgtctcg gctttaaagg agaaacgcaa atcaatgatc aaatgattgg
    10261 ttttggtcac tgggaatatg attttaaagg ctataacgat gaagccaacg gctcgcgcgg
    10321 caacaagacc cgtctggcct atgctggttt aaaaattagt gaatttggct ctctggacta
    10381 tggtcgtaac tacggtgtcg gctacgacat tggttcatgg accgatatgt tgccagaatt
    10441 tggtggcgat acctggagtc agaaagatgt cttcatgaca taccgtacca ccggtctagc
    10501 aacctatcgc aactacgatt tctttggctt aattgaaggg ctgaactttg ccgcgcaata
    10561 tcaaggcaaa aatgaacgta ctgacaacag tcatctttat ggtgctgact acacgcgtgc
    10621 caacggtgac ggtttcggta tctcctcaac ttatgtttat gatggctttg gtatcggtgc
    10681 ggtatatacc aaatccgatc ggacaaatgc gcaggaaaga gccgctgcta atcctctcaa
    10741 tgcctccggt aagaatgcag aactatgggc cacaggtata aagtatgatg ccaacaacat
    10801 ctactttgca gctaattacg ctgaaacatt aaacatgacc acctatgggg atggttatat
    10861 ctctaacaaa gcacaaagtt ttgaagtggt ggcgcaatat caattcgact tcggcttgcg
    10921 cccatcactc gcttacctga aatcgaaagg cagagatctg ggccgctacg gcgaccagga
    10981 catgattgag tatatcgacg ttggtgcgac gtatttcttc aacaaaaata tgtcgaccta
    11041 tgttgattat aaaatcaacc tgattgatga aagcgacttt acccgtgccg tagatattcg
    11101 gaccgataac atcgtcgcta cgggcattac ctatcagttc taacgtcctc tcctgaccag
    11161 cccttctctg ggctggtttt tgatttaagt gctgctgtat gactccttaa gtaaaaatga
    11221 gcaatttttg atatttctgg tcattcacct caattaatat tattccatat cctccgggga
    11281 catcttacta taatatttat tgatgagtga ttatgaccaa tattaataca gcttgtgtaa
    11341 aaaataatgc cagttatcaa cttaataacg cattacctaa caaagaaacc atctccagca
    11401 atttttgtga acgactagaa caatggggta ataaatcggt taataatggt gaagaaagag
    11461 caattgccgt ggagcgaatt aaagaagctt acaattcgaa catggcatct cttgatttgt
    11521 catatcttga tttgagtgaa ttaccgccta tcccctcgac agttaatacg ttaaatctgg
    11581 aaaacaattg tctaacttgt cttgacttag ctgacaatgc cagtctcgtt aatatcaacc
    11641 tcagcgttaa taaaattaac acgataacct ttccaaaaga atcaaaactg gaaaacattt
    11701 atattgatca taacaatctg gaaagtttag atttaaaaaa tcagcattca ttggttaatc
    11761 tggaagcgca aaataacaaa ctgacaaaaa ttaatatttc tgatagttat aaactgaaat
    11821 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
    11881 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
    11941 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
    12001 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
    12061 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
    12121 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
    12181 ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacatcta
    12241 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
    12301 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
    12361 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
    12421 catcagttac ttattttagt gcggcacata
//
LOCUS       DAJTVE010000119        12411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000119 DAJTVE010000000
VERSION     DAJTVE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO439_003215"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO439_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6317507.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO439_003216"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO439_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6317508.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OO439_003217"
     CDS             complement(2659..5772)
                     /locus_tag="OO439_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6317509.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO439_003218"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO439_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6317510.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OO439_003219"
     CDS             7014..7292
                     /locus_tag="OO439_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317511.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO439_003220"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO439_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6317512.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO439_003221"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO439_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6317513.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OO439_003222"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OO439_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6317514.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OO439_003223"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OO439_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6317515.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OO439_003224"
     CDS             10284..10931
                     /locus_tag="OO439_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6317516.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OO439_003225"
     CDS             complement(10973..11503)
                     /locus_tag="OO439_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6317517.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO439_003226"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO439_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6317518.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3420 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcct t
//
LOCUS       DAJTVE010000120        12178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000120 DAJTVE010000000
VERSION     DAJTVE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO439_003227"
     CDS             complement(<1..201)
                     /locus_tag="OO439_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR6317519.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO439_003228"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO439_003228"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6317520.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO439_003229"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO439_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6317521.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO439_003230"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO439_003230"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6317522.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO439_003231"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO439_003231"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6317523.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO439_003232"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO439_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6317524.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO439_003233"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO439_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6317525.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO439_003234"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO439_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6317526.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO439_003235"
     CDS             complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO439_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6317527.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO439_003236"
     CDS             complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO439_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6317528.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO439_003237"
     CDS             complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO439_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6317529.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO439_003238"
     CDS             complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO439_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6317530.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11856..>12178)
                     /gene="flhD"
                     /locus_tag="OO439_003239"
     CDS             complement(11856..>12178)
                     /gene="flhD"
                     /locus_tag="OO439_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6317531.1"
                     /translation="YDINLSYLLLAQRLIVQDKASAMFRLGINEEMATTLAALTLPQM
                     VKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLNDVNQPEEALR
                     KKRA"
BASE COUNT         2777 a         3408 c         2979 g         3014 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtcccc tgcttcgact
     7861 ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
     7921 cgcgcccgga gacgtcggtg acctgctctg ccatggagaa gcctggtgca aatatcagca
     7981 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
     8041 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
     8101 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
     8161 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
     8221 cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc agcgtcagtt
     8281 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaaa
     8341 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
     8401 gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc agttcgctgg
     8461 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
     8521 caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc gttttttccc
     8581 gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
     8641 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
     8701 gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag agcgaatccg
     8761 cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc agcaggtcga
     8821 cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg cgactctgct
     8881 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
     8941 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
     9001 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
     9061 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
     9121 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
     9181 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
     9241 gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct tcatcaaaaa
     9301 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
     9361 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
     9421 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
     9481 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
     9541 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
     9601 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt ctcaccgctg
     9661 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
     9721 acaggcgcaa tgacacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
     9781 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
     9841 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
     9901 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag atcttcgatg
     9961 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    10021 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    10081 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    10141 agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca cgatccatgt
    10201 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    10261 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    10321 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    10381 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    10441 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    10501 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcacccagct
    10561 cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa
    10621 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    10681 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatat
    10741 gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    10801 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    10861 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    10921 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    10981 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    11041 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    11101 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    11161 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    11221 aaaagcagcg gtacgtcgtt accgctactg gaatgttgcg cctcaccgta tcagttaaac
    11281 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    11341 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    11401 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11461 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11521 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11581 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11641 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11701 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11761 gctttccagc atctgcaaac gagcgcccag ggtaatcaat tccattgcca gctgaatatc
    11821 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    11881 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    11941 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    12001 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    12061 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    12121 ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat gtcataaa
//
LOCUS       DAJTVE010000121        11975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000121 DAJTVE010000000
VERSION     DAJTVE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..259
                     /locus_tag="OO439_003240"
     CDS             <1..259
                     /locus_tag="OO439_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317532.1"
                     /translation="HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTII
                     FYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASNVD"
     gene            393..1470
                     /gene="ygjJ"
                     /locus_tag="OO439_003241"
                     /pseudo
     CDS             393..1470
                     /gene="ygjJ"
                     /locus_tag="OO439_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1487..3838
                     /gene="ygjK"
                     /locus_tag="OO439_003242"
     CDS             1487..3838
                     /gene="ygjK"
                     /locus_tag="OO439_003242"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6317533.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4064..6082
                     /gene="fadH"
                     /locus_tag="OO439_003243"
     CDS             4064..6082
                     /gene="fadH"
                     /locus_tag="OO439_003243"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6317534.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6127..6543)
                     /gene="higA"
                     /locus_tag="OO439_003244"
     CDS             complement(6127..6543)
                     /gene="higA"
                     /locus_tag="OO439_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6317535.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6540..6854)
                     /gene="higB"
                     /locus_tag="OO439_003245"
     CDS             complement(6540..6854)
                     /gene="higB"
                     /locus_tag="OO439_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6317536.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7139..8275)
                     /gene="rlmG"
                     /locus_tag="OO439_003246"
     CDS             complement(7139..8275)
                     /gene="rlmG"
                     /locus_tag="OO439_003246"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6317537.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8360..8863
                     /locus_tag="OO439_003247"
     CDS             8360..8863
                     /locus_tag="OO439_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6317538.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8940..9623
                     /locus_tag="OO439_003248"
     CDS             8940..9623
                     /locus_tag="OO439_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6317539.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9702..10688
                     /locus_tag="OO439_003249"
     CDS             9702..10688
                     /locus_tag="OO439_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6317540.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10971..11936
                     /gene="alx"
                     /locus_tag="OO439_003250"
     CDS             10971..11936
                     /gene="alx"
                     /locus_tag="OO439_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6317541.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2848 a         3076 c         3213 g         2838 t
ORIGIN      
        1 tcacctgcca cgcgatttcc gtatgggctc ccgccgcacc ggtattatcg ttgtttcaat
       61 gctgattgcg atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat
      121 tctgaccatc attttttata acgtcggcgg tattgttatc ttccttggct ttgcgtggtg
      181 gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc
      241 agccagcaat gttgattaag cagaataata aaaaatgagc aggcattttc cctcttcctg
      301 gatgaggaga gggaaaatag tttctgcctt tatattttta taattacaac gataaaaggc
      361 tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca ccatgcagag
      421 cattacttgc tctgccgttt tgctacgcct tttctgcggc aggagaagaa gcacgtccgg
      481 cagaacatga cgacacaaaa acacccgcaa ttacctcgac atcttctcct tcatttcgtt
      541 tttacggcga attagggatt agggggtggt ggatatatgg atttagaggg tgagaataaa
      601 cataaataca gcgacggtac ctatattgaa ggtggcctgg agatgaagta cggctcctgg
      661 ttcggcctga tttacggcga aggctggacc gtgcaggccg accacgacgg caatgcctgg
      721 gtgccagacc atagctgggg tggtttcgag ggcggaatta accgtttcta tggcggttat
      781 cgtaccaatg atggcaccga aatcatgctc agtctgcgtc aggattcctc gctggatgac
      841 ctgcaatggt ggggcgattt cacccccgat ctgggctacg tcattcccaa tacccgcgac
      901 attatgactg cgctgaaggt acagaactta agcggcaact ttcgttatag cgtcaccgcg
      961 actcctgccg gacatcatga tgaaagcaaa gcctggctac attttggcaa atacgatcgc
     1021 tatgacgaca aatacaccta tccggcaatg atgaacggtt acatccagta tgaccttgcc
     1081 gaaggcatca cctggatgaa cggtctggaa atcaccgacg gcacaggaca gctctatctc
     1141 acgggcctgc taactcctaa ctttgccgct cgcgcctggc accataccgg acgcgccgac
     1201 gggctggacg taccgggaag tgaaagtggg atgatggtga gcgccatgta tgaagcgtta
     1261 aagggcgttt atctctccac cgcttacacc tacgccaaac atcgccctga ccacgctgac
     1321 gatgaaacca cctctttcat gcagtttggt atctggtacg aatacggcgg cggacgtttc
     1381 gccacggctt ttgatagccg cttctacatg aaaaatgcct ctcacgatcc cagcgaccaa
     1441 atcttcctga tgcaatattt ctactggtaa taaggactgt aatttcatga aaataaaaac
     1501 tattttaacg ccagtaacct gcgctctgct gataagtttt tccgcccatg ccgctaacgc
     1561 cgacaattat aaaaacgtga ttaaccgtac tggcgcgccg cagtacatga aggattacga
     1621 ttacgacgat caccagcgtt ttaatccgtt tttcgatctc ggagcctggc atggtcatct
     1681 gttgccagac ggccctaaca ccatgggcgg ctttccgggc gttgcgctgc tgacggaaga
     1741 gtacatcaac tttatggcca gcaatttcga ccgcctgacc gtctggcagg acggcaagaa
     1801 agtcgacttt acgctggagg catacagtat tcccggtgcg ctggtgcaaa aactgacagc
     1861 aaaagatgtg caagtcgaaa tgactctgcg cttcgccacg ccgcgcacgt cactactgga
     1921 aaccaaaatc accagcaata aaccgctgga tctggtgtgg gacggcgaac tgctggaaaa
     1981 actggaagcg aaagaaggga aaccgctttc cgataaaacc attgctggcg aataccctga
     2041 ctatcagcgc aaaatcagcg ccacccgtga tggcctgaaa gtcacctttg gcaaagtgcg
     2101 cgccacctgg gatctgctga cctccggcga atcggaatat caggtgcata aatccctgcc
     2161 ggtgcagact gaaatcaacg gtaatcgctt taccagtaag gcgcatatca acggttcgac
     2221 cacgctctac accacctatt cccatctgct gaccgctcag gaagttagca aagagcaaat
     2281 gcagatccgc gatattctgg cgcgtccggc gttttatctc accgcctcgc agcaacgctg
     2341 ggaagaatac ctgaagaaag ggttaaccaa tccggatgcg acgccggaac agacgcgcgt
     2401 cgcggtgaaa gcgatcgaaa cgctcaacgg taactggctc tcaccgggcg gtgcggtgaa
     2461 atttaacacc gttacaccgt cggtgaccgg gcgctggttc tccggcaatc agacctggcc
     2521 gtgggatacc tggaagcagg cgtttgcgat ggcgcatttc aatccggaca tcgccaaaga
     2581 gaatatccgc gcggtcttct cctggcagat ccagcctggc gatagcgtgc gtccgcagga
     2641 tgtgggcttt gtccccgacc tgatagcgtg gaaccttagc cccgagcgtg gtggcgatgg
     2701 cggcaactgg aacgaacgta ataccaaacc cagccttgcc gcctggtcgg tgatggaagt
     2761 gtacaacgtc acccaggata aagcctggtt ggcagagatg tacccgaaac tggtggccta
     2821 tcacgactgg tggttacgta accgcgatca caacggcaac ggcgtgccgg aatatggcgc
     2881 gacccgcgac aaagcccaca acactgagag cggcgagatg ctgtttacgg tgaaaaaagg
     2941 cgataaagaa gagacgcagt ctgggctgaa caactacgcc cgcgtggtgg agaaaggcct
     3001 gtacgacagt ctggaaattc cggcacaggt tgctgcatcg tgggaatcgg ggcgtgatga
     3061 tgccgccgtc tttgggttta tcgacaaaga acagctggat aaatatgtcg ctaatggcgg
     3121 caaacgtagc gactggacgg tgaaattcgc cgaaaaccgc agtcaggacg gaacgttgct
     3181 gggctactcg ctattgcagg agtcggtgga tcaggccagc tatatgtaca gcgataacca
     3241 ttatctggcg gagatggcaa ccatcctcgg taagccagaa gaggccaaac gctatcgcca
     3301 gttggcacag cagctcgcgg actacatcaa cacctgtatg ttcgacccga ctacccagtt
     3361 ctactatgac gtacgtattg aagataaacc actggcgaac ggctgcgcgg gcaaaccgat
     3421 tgttgagcgc ggtaaagggc cggaaggctg gttgccgctg tttaacggtg cggcaacgca
     3481 ggccaacgcc gacgcggtgg tgaaggtgat gctcgatcct aaagagttca acacctttgt
     3541 cccgctggga acggcggcgt taaccaatcc ggcttttggc gctgatatct actggcgtgg
     3601 gcgcgtatgg gtggatcagt tctggtttgg tctgaaaggg atggagcgtt acggttatcg
     3661 tgatgatgcc ctgaagctgg cggatacgtt cttccggcac gccaaaggat taaccgccga
     3721 tggcccaatt caggagaatt acaacccgct gactggcgca cagcaaggcg caccaaattt
     3781 ctcctggagt gccgctcatt tgtatatgtt gtataacgat tttttccgta agcagtaatt
     3841 tgcaacggct ggcggatgcg gcgtgaacgc cttatccgcc ctacatgtgc aatcccccag
     3901 gccggataaa acgcggcaag cgttgcatcc ggcgttacca ccgggcgtat tctttttgaa
     3961 tcccatcaca aaccccgcac tccccttttc ccttttctcc atcgacggct aaattagaac
     4021 tcatccgacc acataacaat tattttacat actggacaat tttatgagct atccgtcgct
     4081 gttcgccccg ctggatttag gttttaccac gttaaaaaac cgcgtgttga tgggctcaat
     4141 gcacaccggg ctggaggaat acccggacgg tgccgagcgg ctggcagcgt tttatgccga
     4201 acgcgcccgt cacggcgtgg cgctgattgt cagcggcggc attgcaccag atttaacagg
     4261 cgttggcatg gaaggtggcg caatgctcaa cgacgccagc cagatcccac accatcgcac
     4321 cattaccgaa gcggtacatc aggaaggcgg caaaatagcc ctgcaaattt tgcataccgg
     4381 gcgctacagc taccaaccgc atctggtcgc tccctccgca ttgcaggccc ccatcaaccg
     4441 tttcgttcct cacgaactca cccatgaaga gatcctgcaa ctgatcgacg atttcgccca
     4501 ctgcgcgcaa ctggcacggg aggcaggata cgacggtgta gaggtaatgg ggtccgaagg
     4561 gtatttgatc aacgaatttc tgacgctgcg caccaatcag cgtagtgacc agtggggcgg
     4621 cgattaccgc aaccggatgc gatttgccgt agaagtagtg cgtgcggtgc gcgaacgcgt
     4681 cggcaacgac ttcattatta tctaccgact gtcgatgctc gacctggtcg aagacggcgg
     4741 gacttttgcc gaaacggtag agctggcgca ggccattgaa gcggcgggcg cgaccattat
     4801 caacaccggc attggctggc atgaagcacg tattccgacc attgccacgc ccgtgccgcg
     4861 cggcgcattt agctgggtca cgcgcaaatt gaaaggccac gtctcgctgc cgctggtaac
     4921 caccaaccgg attaacgatc cgcaggttgc cgacgatatt ctctcgcgcg gcgatgccga
     4981 tatggtatcg atggcacgac cgtttcttgc tgatgcggaa ctgctgtcaa aagcgcaatc
     5041 ggggcgagcc gatgagatca acacctgcat tggctgcaat caggcctgcc tcgatcagat
     5101 cttcgttggc aaagtcacct cgtgcctggt gaatcctcgc gcctgccacg aaaccaaaat
     5161 gccaatcctt cctgccgtgc agaaaaaaaa tctggcggtg gtcggtgcgg gacctgctgg
     5221 gctggcgttt gccattaacg cggcggcgcg tgggcatcag gtaacattgt ttgatgccca
     5281 cagcgagatc ggcgggcagt ttaatatcgc caaacagatc cccggcaaag aggagtttta
     5341 cgaaacgctg cgctattacc accggatgat cgaagtgacg ggcgtgacgc tgaaactcaa
     5401 tcacaccgtg acggcggatc agttacaggc gttcgatgaa acgatcctcg ccagtgggat
     5461 cgtgccgcgc gttccgccta tcgacgggat cgatcatccg aaggtattga gttatctcga
     5521 tgtactgcgc gacaaagcgc cggtaggcaa caaggttgcc atcatcggtt gtggcgggat
     5581 tggttttgat acggcgatgt atttaagtca gccgggcgaa tccaccagcc agaatatcgc
     5641 caggttctgt aatgagtggg ggatcgacag cagcctacaa caggctggtg gcttaagccc
     5701 gcagggaatg cagatcccgc gtagcccacg gcaaattgtg atgctccagc gcaaagccag
     5761 caaaccggga caggggttag gcaaaaccac cggctggatc catcgcacca ccctgctctc
     5821 gcggggtgtg aaaatgatcc caggcgtaag ttatcagaag attgacgatg acgggctgca
     5881 tgtggtgatc aacggcgaaa cgcaggtatt agcagtggac aatgtggtga tctgcgcagg
     5941 gcaagagcca aaccgcgcgc tggcgcaacc gctgattgat agcgggaaaa ctgtgcattt
     6001 aattggcggc tgcgatgtgg ctatggagct ggacgcacga cgggcaattg cccagggaac
     6061 acggctggcg ctggagattt aaatcgttat tttaagcgcc ggatgcaacg catccggcac
     6121 gtcgcgttaa tcaataaaca aggcgggaga aatgccgaat cgcgttgcca attttttagc
     6181 gtgttccagc gttaatttcc tcttcccgct caaaacgcgt gacaccatag atttactgcc
     6241 aatttccggc agatcggcaa gggttaaacc atattgatcc ataagggtac gaatcacggc
     6301 tataccgcca ggcatggctt gagccatggc attaaattcc gcaaattcgg gcgctgattc
     6361 ttcccacgcg gttattttgg cacacaccag atccagcaag gggttttcag gatcgttgag
     6421 cagcagatga tctaccagtt ccagcgcctg ggtgtattgt tcctcgttct gaatacccgc
     6481 cagaaaaggt gccaccgcag ttagcttttc tcctgcttgc aagatgtcgg caatcgcaat
     6541 cattttttcc ccttagtacg atgaacagcg gtaaagaaat cgtattcttt atgcgtcata
     6601 acttcacgta tgtagcactt ttgcgattca aaaaagacca ttgctacaac acgtaattca
     6661 ttgcccccaa cattgaaaac ataatgctta tccagatatt tgaagttatc cagagatggg
     6721 aatactgctt ttaatgactc aggttttttg aaatatccct tagcaatcgt gttccccaga
     6781 gccaccaact ccgttttatg ttgcgggtat ttttccgcag catctttcaa tgctttttga
     6841 gttatcaggt gcattcttca tcacgtccgt tgacaaattg gcaatatgat aacatccgtt
     6901 gacagattgg caacggatga atttatctgt gtgacggaag aataaacgta gaaaaacgct
     6961 aaacaagaga atgtagagga tggtgagagg gattttttgt tttatggaag cgcgattcca
     7021 gaactgggca gacattgccg gatgcgacgc ttgccgcgtc ttatccggcc tgggagtaac
     7081 gtgctgaacg taggccggat aaggcgttaa cgccgcatcc ggcaggtaac tcccaatctt
     7141 accgacgacg ccccagcttc actgctttca gcaccacaaa tttattattc gtggcgatgg
     7201 tggtgcagtt gccgaaaatc ttcttcagtt tatggaagta atccaggtga cggttggcaa
     7261 caatatacag ctcgccgttg attttcaggc agcggcgggc atggtggaac atctcccagg
     7321 cgacgttatc ggtcagcgca tgttgttggt gaaacggcgg gttgcagagc acagcattaa
     7381 agcggaaagg ctccacgccg gagagcgcgt tgttgatcat aaactcgctg cgatctaacg
     7441 cctctggcat gttggtttca acgttcaaac ggctggaagc aaccgccatc ggcgattcat
     7501 cgacaaacac cactttcgcc tgagggtttt tatcaagcag cgtcagacca ataacgccat
     7561 taccgcaacc gagatcgaca atctcacctt cgagattctc tggcagatgt tgcataaaga
     7621 agcgtgcgcc aatatccagc ccggtgcggg agaagacatt cgcatggttg tggatagtcc
     7681 agtcagtacc ttccagcttc cagctaacgg tctgcggcgc atcggccagc ggcggttcat
     7741 tgaaagtgca attaatcagg cgcgcttttt tccatgccag cgtagtggtg gtcgggccga
     7801 gcactttctc gaacagctcc agcgtggaag tgtgaatgtc acgggctttg gcaccggcaa
     7861 taatacgtgt atccgacgtg accactttgc gcagcgcacg cagttgctgt tccagcaatg
     7921 ccagtgtttt cggcactttg atcagcacca cacccggctg ttgcgggtag tcggcggtgc
     7981 tgtcgagaaa cttcacgctc gactcatcaa tcccgttgag gcgtaaattc tcgcgcgtcg
     8041 ccagttcact gatgtatgag tcgccaatgc tgtacggctt atgttccgcc agcgcgcagc
     8101 ttaacgcacc aaaggcatca ttcaggatca acaccgggcc gcggatttct gtgtcgtcca
     8161 actgttgcag caaatattca tccgccgctt cccacgcctg tagcgggtta acgtcatccg
     8221 tcgccggaaa acgttgtagt gtcagtgaac ggaaaccgtt gtctaagtgg ctcatcggcc
     8281 ctcctgaatg ataaaatttc gacgttgccc ctgaaaaggg tgcgtgagta tacccttttt
     8341 ctctttttcg tgggcggtta tgagcaatct tacttatctt cagggttatc ccgagcagct
     8401 actttcccag gtgcgaacgc tgattaacga acagcgtctg ggcgatgtgc tggcaaaacg
     8461 ctatccggga acacacgact acgccaccga taaagccctc tggcaatata cccaggatct
     8521 aaaaaatcag tttctgcgca atgccccgcc gatcactaaa gtgatgtatg acaataagat
     8581 ccacgtactg aaaaacgcgc tcggattaca taccgctgtt tctcgtgtgc agggcggcaa
     8641 gctgaaagca aaagcagaga tccgcgtcgc tactgtgttt cgcaacgcgc cggaaccgtt
     8701 tttacgcatg atcgtggtgc acgaactggc gcacctgaag gagaaagagc ataacaaagc
     8761 gttttaccag ttgtgctgcc atatggagcc gcagtaccac cagcttgagt tcgacacccg
     8821 gctgtggcta acgcagttgt cgcttgggca ggacaaaatc tgaaaatgat gtcaggcgtt
     8881 atcttttctt tctttagcgc ctaaaatcga cctcccccct ttcgttaagg ataacgacca
     8941 tgcagccccg ccttcttctc cgtatttgtt tttctcgccg gactctaaag attacctgct
     9001 tactgttact tgtggcagga gcgaccattt ttatcgccga tcgcgtaatg gtcaacgcca
     9061 gcaagcagct gacctggagc gatgtcaacg cagttccggc gcgtaacgtg ggtttattgc
     9121 tgggggcgag gccgggtaat cgctacttta cccgacgtat tgataccgca gcagcgttgt
     9181 accacgtcgg aaaagtgaaa tggctgttag tcagtggcga taacggacgt aaaaattacg
     9241 atgaagcctc aggtatgcag caggctttga tcgctaaagg cgtaccggcg aaagtgatct
     9301 tctgcgacta tgccggattc tcaacgcttg attcagtggt gcgtgccaaa aaggtgtttg
     9361 gggaaaacca tatcaccatt atctcgcaag aatttcataa ccaacgcgcc atctggctgg
     9421 caaaacagta cggcatcgac gctatcgggt ttaacgcgcc agaccttaat atgaagcacg
     9481 gtttttatac tcaactgcgg gagaaactcg ctcgtgtcag cgcggtgatt gacgccaaaa
     9541 tcctccatcg ccagccgaaa tatctcgggc catcggtcat gattgggccg tttagtgagc
     9601 atggctgtcc ggcgaaagaa taatgcgtat ctgcgcacgt cgaagatgaa aaaggcgtgc
     9661 tacattgacg acagaatccc tttatggagt atccacgcgt tatgatacgt ttcgctgtga
     9721 ttggtacgaa ctggatcact cgccagttcg tcgaggccgc ccatgagagc ggtaaataca
     9781 agttaaccgc cgtatattcc cgcagccttg aacaggccca gcacttcgcc aatgattttt
     9841 ctgtcgagca tctgtttacc tcgctggaag cgatggcgga aagcgatgcc attgacgcgg
     9901 tgtatattgc cagcccgaat gccctgcact tttcccagac acaacttttc cttagccata
     9961 aaattcatgt gatttgcgag aaaccactgg cgtcgaatct ggcggaagtg gatgccgcca
    10021 ttgcctgtgc gcgggaaaat caggtggtgc tgtttgaggc atttaaaacc gccagcctgc
    10081 cgaactttca tttgctgcgc caggcgctgc cgaaagtcgg caaattgcgt aaagtctttt
    10141 tcaactattg ccagtattcc tcgcggtatc aacgttacct ggatggtgag aatcccaaca
    10201 cctttaatcc ggcattctct aacggttcaa ttatggatat cggcttttac tgcctggcgt
    10261 cggcggtggc attattcggt gaaccgaaaa gcgtgcaggc aaccgccagt ttgttggcaa
    10321 gcggcgtaga tgcccacggc gtggtggtga tggattacgg tgatttcagc gttacgttgc
    10381 agcactcaaa ggtcagtgat tctgtcctgg aaagcgagat tcagggcgaa gcgggatcgc
    10441 tggtgattga aaaactgtct gaatgccaga aagtgtgctt cgtgccgcgt ggcagccaga
    10501 tgcaggatct cacccagccg cagcatatta ataccatgct ctacgaagca gagctgtttg
    10561 ctaccctggt ggatgagcat ctggtggatc atccagggct ggcggtcagt cgcatcaccg
    10621 ccaaactgct gactgagatc cgccgccaga ctggggtgat ttttccggca gatagcgtaa
    10681 aactataatt gccaaagtaa aacagtgtaa aaggtatgta acagaccatt gactggctga
    10741 atggtctgtc atactttgtt acctgcaaag gggagtaact tcattgccgg tcgatcgtca
    10801 ttacgatgtg tgaaaaacca catccggtca ccgggcaacc cgaaaggaat acgcagacgt
    10861 attccttttt tgttgtaagt gagaccttgc cggaaggcga ggtctatgca taaaaagcag
    10921 cggctgacgt cttccgacgt tggccgtttt tttatgtgta aggaacttct atgaatactg
    10981 tcggcacgcc gttgctatgg ggcggattcg ctgttgttgt cgccattatg ctggctatcg
    11041 acctgttgtt gcaggggcgt cgtggggcac atgccatgac catgaaacag gctgcggcct
    11101 ggtcgctggt ctgggtgacg ctgtcgttac tgtttaacgc cgctttctgg tggtatctgg
    11161 tgcaaactga aggtcgcgcc gttgccgatc cacaggcact ggcctttctc acgggttatc
    11221 tgattgagaa atcgctggcg gtcgataacg tctttgtctg gctgatgttg ttcagctatt
    11281 tctctgttcc ggcggcatta caacgccgcg tgctggtgta tggcgtgctc ggggcgattg
    11341 ttctgcgtac catcatgatc ttcactggca gctggctaat ttcgcagttc gactggatcc
    11401 tgtatatctt cggtgccttc ctgctgttta ccggcgtgaa gatggcgctc gcccatgaag
    11461 atgaatcagg cattggcgac aagccgctgg tgcgctggct acgcggtcat ttgcgcatga
    11521 ccgacaccat cgacaacgag catttctttg tgcgtaagaa tggattgctg tacgccacac
    11581 cgctgatgct ggtactgatt ctggtggaat tgagcgacgt gattttcgcc gtggatagca
    11641 ttccggctat cttcgccgtg accactgacc cgttcattgt gctgacctca aacctgtttg
    11701 cgatcctcgg cctgcgtgcg atgtatttcc tgctggcggg cgtagcagag cgtttctcga
    11761 tgctcaaata tggcctggcg gtgattctgg tgtttatcgg tatcaagatg ctgattgtcg
    11821 acttctacca tattccaatc gccgtctcgc tgggcgtggt gtttggcatt ctggtgatga
    11881 cgtttattat caacgcctgg gtgaattatc ggcatgataa gcagcgggga gaatgattcg
    11941 ctgtatatta agccattcct agagataact cattc
//
LOCUS       DAJTVE010000122        11877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000122 DAJTVE010000000
VERSION     DAJTVE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1760
                     /locus_tag="OO439_003251"
     CDS             <1..1760
                     /locus_tag="OO439_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6317542.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OO439_003252"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OO439_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6317543.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OO439_003253"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OO439_003253"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6317544.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OO439_003254"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OO439_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6317545.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OO439_003255"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OO439_003255"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6317546.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8753
                     /locus_tag="OO439_003256"
     CDS             7437..8753
                     /locus_tag="OO439_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6317547.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8774..9436
                     /gene="csiR"
                     /locus_tag="OO439_003257"
     CDS             8774..9436
                     /gene="csiR"
                     /locus_tag="OO439_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6317548.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO439_003258"
     CDS             complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO439_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6317549.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9970..10128)
                     /locus_tag="OO439_003259"
     CDS             complement(9970..10128)
                     /locus_tag="OO439_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6317550.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10311..10610
                     /locus_tag="OO439_003260"
     CDS             10311..10610
                     /locus_tag="OO439_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6317551.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10620..11144
                     /gene="ygaP"
                     /locus_tag="OO439_003261"
     CDS             10620..11144
                     /gene="ygaP"
                     /locus_tag="OO439_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6317552.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO439_003262"
     CDS             complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO439_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6317553.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2965 a         2928 c         3062 g         2922 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa
     8281 ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc
     8341 gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg
     8401 tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta
     8461 tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc
     8521 gagcggaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg
     8581 tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag
     8641 aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc
     8701 gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat
     8761 caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat
     8821 tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta
     8881 tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt
     8941 cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga
     9001 tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg
     9061 tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc
     9121 ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac
     9181 agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga
     9241 tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct
     9301 ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc
     9361 gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc
     9421 gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat
     9481 ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac
     9541 cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc
     9601 tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat
     9661 tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg
     9721 tcgctttgcc gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc
     9781 tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt
     9841 cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg
     9901 ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg
     9961 agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc
    10021 aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac
    10081 gccgagcggc ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta
    10141 taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg
    10201 gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg
    10261 ttgcattttt agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac
    10321 tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc
    10381 ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc
    10441 tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg
    10501 acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg
    10561 aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta
    10621 tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt
    10681 taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg
    10741 ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta
    10801 tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg
    10861 ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat
    10921 tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg
    10981 cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct
    11041 tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa
    11101 tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg
    11161 cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc
    11221 ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt
    11281 ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc
    11341 gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt
    11401 catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg
    11461 ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag
    11521 aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg
    11581 taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg
    11641 cgtagtgtaa gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg
    11701 cgagattatt tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta
    11761 gccattttga tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat
    11821 tccgcaagcc tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaagaa
//
LOCUS       DAJTVE010000123        11799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000123 DAJTVE010000000
VERSION     DAJTVE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..489
                     /locus_tag="OO439_003263"
     CDS             <1..489
                     /locus_tag="OO439_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6317554.1"
                     /translation="VNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGH
                     HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRR
                     RNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNAR
                     LS"
     gene            541..1476
                     /gene="ttcA"
                     /locus_tag="OO439_003264"
     CDS             541..1476
                     /gene="ttcA"
                     /locus_tag="OO439_003264"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6317555.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="OO439_003265"
     CDS             complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="OO439_003265"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6317556.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="OO439_003266"
     CDS             complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="OO439_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6317557.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4694..5926
                     /gene="dgcM"
                     /locus_tag="OO439_003267"
     CDS             4694..5926
                     /gene="dgcM"
                     /locus_tag="OO439_003267"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6317558.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="OO439_003268"
     CDS             complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="OO439_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6317559.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6840..7748)
                     /locus_tag="OO439_003269"
     CDS             complement(6840..7748)
                     /locus_tag="OO439_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317560.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7924..9234
                     /gene="abgA"
                     /locus_tag="OO439_003270"
     CDS             7924..9234
                     /gene="abgA"
                     /locus_tag="OO439_003270"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6317561.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9234..10679
                     /gene="abgB"
                     /locus_tag="OO439_003271"
     CDS             9234..10679
                     /gene="abgB"
                     /locus_tag="OO439_003271"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6317562.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10716..>11799
                     /locus_tag="OO439_003272"
     CDS             10716..>11799
                     /locus_tag="OO439_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6317563.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFN"
BASE COUNT         3002 a         2997 c         2924 g         2876 t
ORIGIN      
        1 gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa aacaaatgcc
       61 gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga gcaatacaaa
      121 ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta cggcagaact
      181 gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa acagaaacct
      241 tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg tgctggtatt
      301 cgccgccgta atccgtacca tacgcgacat acttttgcct gctggctgtt gacggcagga
      361 gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat ggtgtatgaa
      421 atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt gaatgcgcgg
      481 ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag aactgaaata
      541 atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa attacaaaaa
      601 cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat
      661 cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga gattctgcgc
      721 aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag
      781 caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac
      841 aagattgtcg aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc
      901 acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg
      961 ggggcgacga agatcgcgct gggtcaccat cgtgacgata tcctgcagac gctgttctta
     1021 aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc
     1081 aaacatatcg ttattcgtcc gctggcctac tgccgcgaga aagatattca gcgatttgcc
     1141 gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa
     1201 cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag
     1261 acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc
     1321 gatttcaaag gcatcaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat
     1381 cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg
     1441 gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatggcgccc gatgcgcaag
     1501 cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc
     1561 ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc
     1621 acccggctcg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     1681 acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct
     1741 gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg
     1801 gcttttttcc caccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa
     1861 ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     1921 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     1981 gctgtacggc cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     2041 accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc aaccagtaca
     2101 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccaaatcg
     2161 ccgtgtaatg acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa
     2221 tcttttttgg tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt
     2281 tgcaacagag gaattttgcc tttgctggat gtctcataaa attgttgttc aatgggtggc
     2341 aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt
     2401 acggcaatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca
     2461 aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca
     2521 tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc
     2581 aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata
     2641 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     2701 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     2761 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     2821 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     2881 gcggcggcct gcaccggcgt catagttaaa taacccaact cattaaggtt cgtgagttgg
     2941 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     3001 cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     3061 gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3121 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3181 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3241 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     3301 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     3361 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     3421 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acggtgcaac
     3481 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     3541 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta
     3601 ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc taaattttcc
     3661 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     3721 tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     3781 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     3841 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     3901 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     3961 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4021 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4081 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4141 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4201 ctgactcggg gccgcgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
     4261 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     4321 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     4381 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     4441 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     4501 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     4561 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     4621 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     4681 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     4741 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     4801 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     4861 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     4921 tgttcagcgt aaagaagagg aaacagcatt aaactgtcgg cttgttttgc gaaagctgac
     4981 agaagcagaa ccggtgatta ttttcgaagg tatcgaagcg tcggcaacgc tgggtttaaa
     5041 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     5101 gactaactct gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc
     5161 taacctcgcc gcgctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac
     5221 ctgggaaata aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
     5281 acccggtggt cataaacctc tgaattttat tcataagctg gcggatggtt cgactcgtca
     5341 tgtgcagacc tacgccgggc cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     5401 acatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga
     5461 cgcgatgacc ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat
     5521 gcagcatctc gccatcgccc aggattacag cttgttgctc atcgacaccg atcgttttaa
     5581 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
     5641 caccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     5701 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     5761 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt
     5821 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     5881 gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc
     5941 tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
     6001 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata
     6061 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     6121 atgcgattta tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatcagc
     6181 catggcttgt tgaatgaaac tgaacatcat tttgcggcac tcttccaccg gctggcgcaa
     6241 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     6301 ttgcaaccct tcccgccgaa actccagcgg ctgacttagt gggatgatgt cgagaaatcc
     6361 ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     6421 cgttggatgc cagtggacat cggtagcacg tttcagcggc tggacatctt ccatggcgtc
     6481 aagaaacagc gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat
     6541 atgataacca gaccgggtcg gtccaacaac gtattaccca aaattccagt aataagttcc
     6601 aaatattgcc gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat
     6661 aatgtaactt atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa
     6721 accgttataa cacagtcacc ggcgcagagg agacaatgcc ggatttaaga cgcggatgca
     6781 ctgctgtgtg tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt
     6841 taactttgca gatagccgca ttctcgccgg aattgcgtga ttaatgacgc ggtcagtggt
     6901 gtctggcgac tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttcgcta
     6961 accggcaaca tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt
     7021 atgctgagaa aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact
     7081 acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtaa
     7141 ctgccgtgtg gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca
     7201 ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca
     7261 aaagtaaatt cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct
     7321 ccctgacgca attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc
     7381 tgcggatgct gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg
     7441 gcccccatgc cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatatcctct
     7501 tgggctgcgc gcagctcttc aagaataaga ctggcgtgct gataaaaact ttcaccggca
     7561 tcagttaacg tcacgccttt actacggcga aaaaagagtt gcgccgctaa tccttcttcc
     7621 agctcctgaa tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg
     7681 cgaatgctgc cctgacgagc cacttcaaca aaagcccgaa tttgatgaat ttttacctga
     7741 aaagccatga cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt
     7801 aatgcagatg attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag
     7861 tgataagtaa aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag
     7921 gttatggagt ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga
     7981 cgtgattttc atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt
     8041 gcggaagaat tgcagcagct cggctattca ctggcgctgg gccgcgaagt agttaatgaa
     8101 agtagccgga tgggactacc tgatgaactc actctacaac gcgaattcga gcgcgctcgt
     8161 caacagggtg cgctagcaca atggattgcg gtttttgaag gtggtttcac tggtatcgtc
     8221 gccaccctgg ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg
     8281 ctggatctca gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca
     8341 tgtaacgccg gaatgatgca tgcctgtggt catgatggac ataccgccat tgggcttggg
     8401 ctggcgcata cccttaaaca attcgagtcc ggactacatg gcgtcatcaa actgattttt
     8461 cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat
     8521 gatgttgatt attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg
     8581 tgcggcagtg ataattttat ggcaaccacc aaatttgacg cgcacttcac cggtaccgcc
     8641 gctcacgcag gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagcc
     8701 actcttgcac tgcatgcaat cgccccgcac agcgaaggag cttccagagt aaacgtgggc
     8761 gttatgcagg caggaagcgg tcgtaacgtt gttcctacct cggcgttgct gaaagtggaa
     8821 acacgcgggg ccagcgacgt cattaatcaa tatgtttttg accgtgcaca acaagcgatt
     8881 cagggcgcag caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagttacc
     8941 gccagttctc cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg
     9001 ggggtcaatc aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta
     9061 atgatggccc gcgtgcagca acatcaaggg caagcctcct acgtggtgtt tggcacacag
     9121 ctggcggcag gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc
     9181 gtcgaaacgc tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg
     9241 aaatctatcg ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg
     9301 ccgatcaaat ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc
     9361 tggcttcggc tctggaatct gcaggcttca ccgttacccg caacgtaggc aatatcccaa
     9421 atgcctttat tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg
     9481 acgccctggc aggtttaagt cagcaagctg gttgcgcgca acctacatcc gcgacgcccg
     9541 gtgaaaatgg tcacggttgc ggacacaatt tgcggggaac cgccgccttt gccgctgcaa
     9601 tagccgtcaa gaaatggctg gaacaatatg ggcaaggcgg cacagtgcgc ttttatggtt
     9661 gtcctggcga agaaggcggc tcgggtaaaa cgttcatggt ccgcgagggg gtatttgatg
     9721 atgtggatgc ggcactcacc tggcacccgg aagcctttgc cggtatgttc aatacccgca
     9781 cgctggcaaa cattcaggca tcatggcgct ttaaagggat cgcagcacat gccgcgaatt
     9841 cccctcattt gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact
     9901 tcctcaacga acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg
     9961 ggatctcgcc caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg
    10021 aaatgaccga cgtgcagcat atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat
    10081 tgatgaccga aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga
    10141 atcgcacctt agaaaatgcc atgtaccatg ccctatccca ttttggtacc ccggaatgga
    10201 actccgaaga actggctttt gcgaaacaaa ttcaggctac gctcaccccc aacgatcggc
    10261 aaaacagtct gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac
    10321 gtcatcgtga aacggtactg gcgaatgagg tcgctccata tgccgccacc gataacgtgc
    10381 ttgcggcatc gactgatgtc ggcgacgtta gttggaaact gcctgttgcc cagtgtttca
    10441 gcccctgttt tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa
    10501 catctattgc tcataaagga atgctgctgg cggcgaaaac tatggcagca accacagtca
    10561 atctcttcat tgattcaggg ctattgcaag aatgccaaca agagcatcag caagtaacgg
    10621 acacgcaacc gtatcactgc cctatcccga aaaacgtgac accgtcacct ttaaaataac
    10681 aacaacaacg caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt
    10741 cctcccaatc cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttctccatc
    10801 cttttttgct ctttatctat ttgattatcg tactcatggt gacgacggca attttgtcgg
    10861 cctttggcgt cagtgcgaaa aacccgaccg atggaacgcc ggtggtggtg aaaaacctgc
    10921 tcagtgtgga aggattacac tggtctttac ccaatgttat taaaaacttt agcggttttg
    10981 ctccacttgg tgcgatcctg gcgctggttt taggtgccgg tctggcggag cgcgtcggct
    11041 tactgccagc gctaatggtt aaaatggcat cgcatgttaa tgcccgctac gccagttata
    11101 tggtgctgtt tattgctttt ttcagccaca tttcttccga tgcgacgtta gtgatcatgc
    11161 taccgatggg tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg
    11221 ctgccattgc aggcgtaggt tgcggcttta cggctaattt actgattgtc acaaccgacg
    11281 tgttgctgtc ggggatcagc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca
    11341 gtgtaattga taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg
    11401 gcctgataac cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg
    11461 agaaactgca gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat
    11521 cgctactttt tattgctgcg attgcgctga tggtgatccc ggaaaacggg atattgcgcg
    11581 atccgattaa tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactgatca
    11641 ttcttttttt ctttgttgtc tcgctggctt atggcatcgc tacccgcaca attcgacgtc
    11701 aggcggattt accgcagtta atgattgaac cgatgaaaga gatggcagga tttatcgtga
    11761 tggtttttcc cctcgcccag tttgtcgcca tgtttaact
//
LOCUS       DAJTVE010000124        11543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000124 DAJTVE010000000
VERSION     DAJTVE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            461..964
                     /locus_tag="OO439_003273"
     CDS             461..964
                     /locus_tag="OO439_003273"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6317564.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1337..2530)
                     /locus_tag="OO439_003274"
     CDS             complement(1337..2530)
                     /locus_tag="OO439_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6317565.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2666..4390
                     /gene="iucA"
                     /locus_tag="OO439_003275"
     CDS             2666..4390
                     /gene="iucA"
                     /locus_tag="OO439_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6317566.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4391..5338
                     /gene="iucB"
                     /locus_tag="OO439_003276"
     CDS             4391..5338
                     /gene="iucB"
                     /locus_tag="OO439_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6317567.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5338..7080
                     /gene="iucC"
                     /locus_tag="OO439_003277"
     CDS             5338..7080
                     /gene="iucC"
                     /locus_tag="OO439_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6317568.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7077..8414
                     /gene="iucD"
                     /locus_tag="OO439_003278"
     CDS             7077..8414
                     /gene="iucD"
                     /locus_tag="OO439_003278"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6317569.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8420..10615
                     /gene="iutA"
                     /locus_tag="OO439_003279"
     CDS             8420..10615
                     /gene="iutA"
                     /locus_tag="OO439_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6317570.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11166..11309)
                     /locus_tag="OO439_003280"
     CDS             complement(11166..11309)
                     /locus_tag="OO439_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317571.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2896 c         2949 g         2771 t
ORIGIN      
        1 tctcaacgaa ggggccgaca aacacgttgt ccctgagttc acattcataa atgttgaccg
       61 gtttgacaat acggacattg atcccataaa tcacatcccg gacagcacat tccagaaatt
      121 tcatactcac acgtccttta aatacagatg acatttgccg gaattatacc gcccagattc
      181 acttgccatg tttcagaata gcttataagc cgcactttta ccgatactca gcaacaaatc
      241 aggtctgtat ccacttcagc cggaaaccgg atctgcgcgg cggggaacgg gcaagaaata
      301 tgataattaa gcaacacaca taaaatcata ttgcataatc gcatctgaat cgaacactta
      361 ttcgcatcac tatcgatcat cacttcgcat ctcataccct tacccacaag ttgaaataat
      421 agtattatat tgaaaagaca gagattattt cagaggtgtt atggaaaact gggttattca
      481 ggagctaaaa tcactggata taggggactc aagacttgat aaaagagtaa aacatatcct
      541 caatagcctc agtcgttcac cagaagaaag tattcctgtt tcctgccgta cctggagtga
      601 aacaaaggca gcttatcgtt gtttttcaaa cgataaagtc tctgctaata aaattatggc
      661 tcctcacaaa gaaaatatca ctgaaagagc tcagcagttt aaaagagttt tagttttaca
      721 ggatacaaca gaacttaatt attctggtca gaaagaaaaa caaggagttg gtcacaaaaa
      781 acataaagat gaaagaaact tatttcttca tcctcaactc gtcatcagtg agtcaggtgt
      841 ttgcctcggt gtttatgatg actatcagtg gttcagggat gaattaaaaa ccagtaaaaa
      901 tacccgacaa gaaatctgca atgatttact tcataaaaaa catgtcagtg aaaaagaaac
      961 ctagcgttgg gttgaaggat ataataaagc aacagaactg gctcgccaat gccctgatac
     1021 ccatgtattg tcaatttctg atcgtgaagg cgatttttat gacctgtttg aacgcgcaga
     1081 gcagactccc ggaataaaag ctgactggct tgtcagaatg aaatttaata acagagcaac
     1141 gctgaatatt aatggtaaac gggatcatag gttgttgcac gaaagaatca tggagattac
     1201 accgcagcaa ctggttgaat tcactatacc tgatgggcgg gggcagcaag ccagaacact
     1261 gcctgacatg ctttcatcag acgcaggtcc tctctcatat cctctgattt actccagtaa
     1321 ggtgatgatc atatctttat ttgatagtac ggtaatgcca gataaataca gacacaatgg
     1381 taaataatat agttgcaaat aatgctgcca taatcagcgc gattgcgaca cctgttttcg
     1441 ataccagcgg caccagcaaa gcgcacagtc catagccaag tgtgtgactg gtagcgagcc
     1501 agcccgcgcc tgcgccatca gccagcttat cattcagtag aagttgataa gcgggtgttg
     1561 ccagagctgc tccaaatgac agcactgcac agcccgggta aaacaaccat agctgttcgg
     1621 agagcatgat agccagacca ccactcatca acactccggc actcaggagc agggccaccg
     1681 gagtcaggcg ctgcggacgg agtaccccga actgcgcgat aagcgcagct actgcggaca
     1741 gacccaacag ccaggccacc tgttggctaa tggcggtggt atccgtgacg aactggcgag
     1801 taagggcagg cgaaagtcca agctgcatca tgctcaccgc cgcagccagt aatattgcgc
     1861 aaagcagata aggcagacaa tcccgcttca gactgacgct cttgcactcc ggtgtgggct
     1921 gtggtggtgt gccgggcaac cgcagcagca tcagcagcgc cagcactggt gctgccatca
     1981 gtagccccag tggcgccaga ggatgaatgg ccagcattgc tgccgcgcac agcggcccga
     2041 atagcctgcc gcaactcagg ccggagctga tggttgccag agcggccatg cgattccctt
     2101 ctccagctct ctgcaatgcc cagacctgac aggctggcac cattgccgac acggtcagcc
     2161 cgtaggcgat ccgggcgatg attaatattc ccagtcccac cgctgttgtt atggcgctgg
     2221 tagccatcag cacgcttccc agccctagca gggtaaagct tatgccatat cctcccagag
     2281 cccacaacac cacaggttta ctgccagtac gggaaatttg ctttccccac cacggggacg
     2341 atggcagaaa cagcattgag ccaagcatca gcagggcagc ccatacagac agactcagat
     2401 ttgtctgtat gaccagaaca gggagcacaa ccagcagacc gttctgtccg ataccgagaa
     2461 gcccggcact gaacgcaagt ggccagcagg acagtgtttt ttggggcgta tcttcgatcc
     2521 ctggtgacat tataatgttt caactccatg tattaattgt gtttatttgt aaaattaatt
     2581 tatctgacaa taacatttat tgttgataat gagaatcatt atcgacataa ttgttattat
     2641 tttactgtgt gggagctgtt tgactatgac cctgccctct gaaaaaccag ccacagatgt
     2701 ggctgcgcag tgcttcctga atgcactgat tcgtgaaacc acagactgga aactgacaga
     2761 atacccgcca gacgaattgc tcatcccgct ggatgagcag aaatcgctcc atttcagagt
     2821 ggcttatttc tccccaaccc agcatcaccg ctttgcattt cctgcacgtc tggtcacggc
     2881 atcaggcagt tatcctgtcg actttaccac tctctcccgg cttattattg ataagctacg
     2941 ccatcaactg tttctgcccg ttcccctctg cgaaactttc caccagcgcg tgctggaaag
     3001 ccatgtccat acgcaacagg caattgatgc ccgtcatgac tgggccgctc tgcgtgaaaa
     3061 agcgttgaat tttggcgagg ctgagcaggc gctgctgaca ggacacgctt tccaccctgc
     3121 gcctaagtct catgaaccgt ttaaccgacg ggaggctgaa cgctacctgc ctgatatggc
     3181 accccacttc ccactgcggt ggttttcggt ggataaaacg caaatcgccg gtgaaagttt
     3241 gcatctcaac cttcaacagc ggctgacgcg atttgccgca gagaatgcgc ctcagttact
     3301 caacgaatta agtgacaacc aatggctgtt cccgttgcac ccgtggcagg gggaatatct
     3361 tttgcagcag gggtggtgcc aggcacttgt tgctaaaggg ctgattaaag acttaggtga
     3421 ggccggcacg tcgtggctgc cgaccacctc ttcccgttcc ctctactgtg ccaccagccg
     3481 cgatatgatc aagttctccc tgagcgtacg gctgaccaac tccatccgta ctctgtccgt
     3541 gaaagaagtg aagcgtggga tgcgcctggc acgcctggct caaaccgacg gctggcagat
     3601 gctacaggtc cgcttcccga ctttccgggt aatgcaggag gatggctggg ccgggctgct
     3661 cgatcttaac ggcaacatca tgcaggaaag tctgtttgcc ctgcgtgaaa atctgctggt
     3721 ggatcagccg aaaagccaga ctaacgtact ggtctccctg actcaggccg cacctgatgg
     3781 cggtgattcg ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt
     3841 gcaacaggcc gcccatgcat gggtcgatgc atactgtcag caagtgctaa agccgctgtt
     3901 tacggctgaa gcggattacg gcctggtgct gctggcgcat cagcaaaata ttcttgtcca
     3961 gatgcttggg gatctgccgg tcggatttat ttaccgtgac tgtcagggca gcgcttttat
     4021 gcctcacgcg acagactggc tcgattctat tggcgaggcg caggcggaaa atattttcac
     4081 ccatgagcag ttgctgcgct atttccctta ttacctgctg gttaactcca cttttgctgt
     4141 gaccgctgcg ctgggggctg ccgggctgga cagcgaatcg aatctgatgg ctcgtgtacg
     4201 agcatcgctg gctgaagtgc gtgatcaggt gactcataaa acctgtctca actacgtgct
     4261 ggaaagtccg tactggaacg taaaaggtaa ctttttctgt tatctgaacg atcataacga
     4321 gaacaccatt gttgaccctt cggtgatcta tttcgatttc gctaacccgc tgcaggctca
     4381 ggaggtctga atgtctgagg caaacattat tcacagcaga tatggactgc gctgtgaaaa
     4441 actcgacaag cccctgaatc ttggctgggg actggacaat agcgcggtgt tgcactgtcc
     4501 cggggagctg ccgacagggt ggctgtgcga tgcgctggat cagatattta tcgccgcacc
     4561 acaactgtca gcagtggctc tcccctgggc cgaatggcgt gaggagccac aggcgctgac
     4621 gctttttggg caggtaaaaa gcgacattat ccatcgtact gccttctggc agttaccgtt
     4681 atggttgagt tcaccggcaa accgggcctc cggtgaaatg gtttttgatg cagagcgtga
     4741 gatttatttc ccgcagcgcc caccccgtcc acagggtgag gtttaccgcc gttacgatcc
     4801 acgtattcgc aggatgctga gtttccgcat tgccgatccc gtttctgatg cagaacgttt
     4861 cacccgctgg atgaatgatc cgcgcgttga gtatttctgg gagcaaagtg gctcactgga
     4921 ggtacagacc gcctatctgg agcgccagct caccggtaaa catgcattcc cgctgattgg
     4981 gtgcttcgac gatcggccat tcagctattt cgaaatctac tgggcggcgg aagaccgcat
     5041 tggtcgtcac tattcgtggc agccctttga ccgtggcctg catctgctgg ttggtgaaca
     5101 gcaatggcgc ggggcccact atgtgcaaag ctggctgcgc gggctgacac attacctgct
     5161 gctggatgag ccccgtacgc agcgcacagt actggagcca cgcaccgata accagcgcct
     5221 gttccgccat cttgagcctg cgggataccg gacaattaaa gagttcgact tcccacataa
     5281 gcgctcgcgc atggtgatgg cggatcgtca tcacttcttc acggaggtcg gtctgtaatg
     5341 aatcacaagg actgggattt tgttaaccgt cggctggtgg caaaaatgtt gtctgagatg
     5401 gagtatgagc aggttttcca cgcggaatct cagggcgatg accactactg cattaacctg
     5461 ccgggagcac aatggcgctt catcgctgaa cgtggtatct ggggctggct gtggattgat
     5521 gcccaaacgt tgcgctgcac ggacgaacca gtactggcgc agacgctgct gatgcagcta
     5581 aagccggtgc tgtcaatgag tgatgccact gttgcagagc atatgcagga tttgtatgcc
     5641 acgctactgg gcgacctgca actgctgaag gcccgtcgcg gactgagtgc cagtgacctg
     5701 attaatcttg atgccgatcg tctgcaatgc ctgctgagcg gtcatcctaa attcgttttt
     5761 aataaaggtc gccgtggctg gggcaaagag gcgctggaac gatatgcgcc agagtatacc
     5821 aacaccttca gactgcactg gctggcggta aaacgtgaac atatgatctg gcgctgcgat
     5881 aacgatctgg atattcagca gttgttgact gccgcaatgg atccgcagga gtttacacgc
     5941 ttcagtcagg tctggcagga aaacgggctg gatcacaact ggctaccgct gccggtacat
     6001 ccgtggcagt ggcagcaaaa aatcgccaca gacttcatcg ctgattttgt cgaaggcagg
     6061 atggtgtctc tcggcgagtt tggcgaccag tggctcgccc agcagtcgct gcgtaccctg
     6121 accaacgcca gccgccgggg agggctggat atcaagctgc cgctgaccat ctacaacacc
     6181 tcatgctacc ggggaatccc tggcagatat atcgctgccg gaccactggc ttcacgctgg
     6241 ctgcaacagg tttttgcgac cgacgccacc ctggtgcaaa gcggcgcagt gatccttggt
     6301 gaaccggctg caggctatgt gtcccatgaa ggctatgccg cgcttgcccg ggctccctat
     6361 cgctaccagg aaatgcttgg tgttatctgg cgggagaatc cgtgccgctg gctgaaaccg
     6421 gatgaaagtc ctgttctgat ggcaacactg atggagtgcg acgaaaacaa tcagccgctg
     6481 gcaggcgcat atatcgaccg ctccggactg gacgctgaaa cctggcttac gcaattgttc
     6541 cgggtggttg tggttccgct gtatcacctg ctttgccgct acggtgtcgc gcttattgca
     6601 cacggacaaa atataacgct cgccatgaaa gagggggtac cacagcgtgt tctgctgaaa
     6661 gacttccagg gcgatatgcg gctggtgaaa gaagcgtttc ccgaaatgga ctctttgcct
     6721 caggaggtgc gtgatgccac atcccgtctg agtgcggact acttaatcca tgatttgcag
     6781 acgggtcact tcgtgacagt actgcgtttt atttcgccac tgatggttcg tcttggcgta
     6841 cctgaaaggc gattttatca actgctggca gcagtgttga gtgattacat gaacaaacat
     6901 ccacaaatgg cagagcgttt tgcgcttttc tcactcttca ggccacaaat cattcgcgtg
     6961 gtgctgaacc cggtaaaact gacctggccg gacctggatg gcggcagtcg catgctgccg
     7021 aattaccttg agaatctgca aaatccgctg tggctggtaa ctcaggaata tgaatcatga
     7081 aaaaaagtgt cgattttatt ggtgtcggga cagggccatt taatctcagc attgctgcat
     7141 tgtcacatca gatcgaagaa ctgaactgtc tcttctttga cgaacatcct catttttcct
     7201 ggcatccggg tatgctggta ccggattgtc atatgcagac cgtctttctg aaagatctgg
     7261 tcagtgcagt tgcacctaca aatccctaca gctttgttaa ctatctggtg aagcacaaaa
     7321 agttctatcg cttccttaca agcaggctac gtacagtatc ccgtgaagag ttttctgact
     7381 atctccgctg ggctgctgaa gatatgaata acctgtattt cagtcatacc gttgaaaata
     7441 ttgatttcga caaaaaaagt cgattgtttc tggttcagac cagtcgggga gaatattttg
     7501 cccgcaatat ctgccttggt acaggaaaac aaccttattt accaccctgt gtgaagcatg
     7561 tgacacaatc ctgtttccat gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa
     7621 aacggataac cgtggttggt ggaggacaga gtggtgcaga cctgttcctt aatgcattac
     7681 gcggggaatg gggagaagcg gcggaaataa actgggtctc acggcgtaat aattttaacg
     7741 cactggatga ggctgctttt gctgatgagt attttacacc tgaatatatt tcaggcttct
     7801 ccggactgaa ggaagatatt cgccatcagt tactggatga gcagaaaatg acatcggatg
     7861 gcatcactgc cgattcttta ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc
     7921 tgagaaaacc aagaaatatc cgtctgctac ccagtcgctc ggtaacaacc ctggaaagta
     7981 gtggtccggg ctggaggtta ttgatggaac atcatctgga tcagggcaga gagagcctgg
     8041 aaagtgatgt ggtgattttc gccacaggtt accgttctgc attgccacaa atacttccct
     8101 cactgatgcc cttgatcacc atgcacgata agaacacctt taaagtgcgt gatgacttca
     8161 ctctggaatg gagtggcccg aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa
     8221 ctcatggcat cgccgaaccc cagctcagcc tgatggcctg gcgatctgca cgtattctta
     8281 atcgcgtact ggggcgtgat ttattcgatc tcagtatgcc gcccgcactg attcagtggc
     8341 gcagcggcag ccggaaaaaa ccgcagccgg aggctgcttc tttaactcac tatacagcaa
     8401 atattcagga ataacgatga tgcgcaaaaa gtatatgccc cgggctcttg gtccgctgct
     8461 tcttgtcgtg ctgtcaccag ctgtcgccca gcaaaacgat gataatgaga tcatagtgtc
     8521 tgccagccgc agcaatcgaa ctgtagcgga gatggcgcaa accacctggg ttatcgaaaa
     8581 tgccgaactg gagcagcaga ttcagggcgg taaagagctg aaagacgcac tggctcagtt
     8641 aatccccggc cttgatgtca gcagccagag tcgaaccaac tacggtatga acatgcgtgg
     8701 ccgcccgctg gttgtcctga ttgacggtgt gcgcctcaac tcttcacgtt ccgacagccg
     8761 acaactggac tctgtcgatc cttttaatat cgaccatatt gaagtgatct ccggcgcgac
     8821 ggccctgtac ggtggcggga gtaccggagg gttgatcaac atcgtgacca aaaaaggtca
     8881 gccggaaacc atgatggagt ttgaggctgg cacaaaaagt ggctttaaca gcagtaaaga
     8941 tcacgatgag cgcattgccg gtgctgtctc cggcggaaat gaccatatct ccggacgtct
     9001 ttccgtggca tatcagaaat ttggcggctg gtttgacggt aacggcgatg ccaccctgct
     9061 tgataacacc cagaccggcc tgcagcactc caatcggctg gacatcatgg gaaccggtac
     9121 gctgaacatc gatgaatccc ggcagcttca actgataacg cagtactata aaagtcaggg
     9181 agacgacaat tacgggctta atctcgggaa aggcttttcc gccatcagcg ggagcagcac
     9241 accatacgtc agtaaggggc tgaattctga ccgcattccc ggcactgagc ggcatttgat
     9301 cagcctgcag tactctgaca gtgatttcct gggacaggaa ctggtcggtc aggtttacta
     9361 ccgcgatgag tcgttgcggt actacccgtt cccgacggta aatgcgaata aacaggcgac
     9421 agctttctcc tcgtcacagc aggataccga ccagtacggc atgaaactga ctctgaacag
     9481 ccaactgatg gacggctggc aaatcacctg ggggctggat gctgagcatg agcgctttac
     9541 ctccaaccag atgttcttcg atctggctca ggcaagtgct tccggagggc tgaacaacca
     9601 taagatttac accaccgggc gctatccgtc atatgacatc accaatctgg cggccttcct
     9661 gcaatccagc tatgacatta atgatatttt taccgttagc ggtggcgtac gctatcagta
     9721 tactgagaac agggtagatg atttcatcga ctacacgcag caacagaaga ttgctgccgg
     9781 gaaggcgata tctgccgacg ccattcctgg tggttcggta gattacgata actttctgtt
     9841 caatgctggt ctgctgatgc acatcaccga acgtcagcag gcatggttca atttttccca
     9901 gggggtggca ttgccggatc cggggaaata ttatggtcgc ggcatctatg gtgcagcagt
     9961 gaacggccat ctttccctga caaagagcgt gaacgtcagc gacagtaagc tggaaggcgt
    10021 gaaagtcgat tcttatgaac tgggctggcg ctttaccggt gacaacctgc ggactcaaat
    10081 cgcggcatat tactcgcttt ccaataagag cgtggaaagg aataaagatc tgaccatcag
    10141 tgtgaaggac gacaggcgcc gtatttacgg cgtggaaggt gcggtggact acctgatccc
    10201 ggatactgac tggagtaccg gtgtgaactt caatgtgctg aaaaccgagt cgaaagtgaa
    10261 cggtcaatgg caaaaatatg acgtgaagga atcaagtcca tcgaaagcga cagcttacat
    10321 taactgggcg ccggaaccgt ggagtctgcg tgtacagagc accacttctt tcgacgtaag
    10381 cgatgcagag ggtaacgata ttaatggtta cactaccgtc gattttatca gtagttggca
    10441 gcttccggtg ggaacactca gcttcagcgt tgagaacctc ttcgaccgtg actataccac
    10501 tgtctgggga cagcgtgcac ctctgtacta ctgcccgggt tacggccctg cttcactgta
    10561 cgactacaaa ggccggggcc gaacctttgg tctgaactac tcagtgctgt tctgacgggg
    10621 atgcttttgc aacaagggta tgctgtaaac aacatggtcc ttggtggcca tgttgtttac
    10681 agccagtata atatacagcc ttaacaagat attccgtctc cggaatggac tgttcaccac
    10741 tgaataacat tatttctgtt ttatggatct ttgactgaaa tttatcgcat atcctcattt
    10801 cattgtccgt ctgaaaacta accttacaag taagcgtcgt ctcagcaccg tctggcagat
    10861 cctgaaattc ctgagaggat gatctgcgcc caatatcctg agccggaact ttgctgcaat
    10921 gaaggccaac gaaaaatggg ggagtatgtt atagagtact tgctacaggg taaaaagagg
    10981 tacctgtcgc tggttctccg ttcagtttaa ccgggagatt ttctcctgcg cctgtcataa
    11041 agtccggtaa tggagattgt taatacgatg gtgcacgatg cattgtcggt gcccggcccg
    11101 gaggatgccc cgtaactaca tacggatcag ggttggcaat atcgaatggc aggctatagg
    11161 caaagttaaa gccccatgga gtagcacaaa atatgccaca caaaggaaac ggtctgaata
    11221 acgcagtgat ggagaacttc ttcggcacac tgcaaaatcg catcgtggtc gatcgctaat
    11281 tcgccgtgaa tcactgaacg atgttccatg cgaacagata ttcgatcata tcgaaatatt
    11341 aaacattacc cctccccgca ttgggcaaaa aaaaaacaaa aaacgatcaa ttagtattga
    11401 ttcacataat ctctcatcat taatgagaac attgtcacat atttacaata tagaactgtt
    11461 acttttagta agctgttagt aaccaactgt tactaataat tttaattctt aatactttgt
    11521 tcattcaata aatgagttga gag
//
LOCUS       DAJTVE010000125        11258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000125 DAJTVE010000000
VERSION     DAJTVE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO439_003281"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO439_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6317572.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="OO439_003282"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="OO439_003282"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6317573.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="OO439_003283"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="OO439_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6317574.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="OO439_003284"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="OO439_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6317575.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="OO439_003285"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="OO439_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6317576.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="OO439_003286"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="OO439_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6317577.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="OO439_003287"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="OO439_003287"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6317578.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="OO439_003288"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="OO439_003288"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6317579.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="OO439_003289"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="OO439_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6317580.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="OO439_003290"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="OO439_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6317581.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="OO439_003291"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="OO439_003291"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6317582.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="OO439_003292"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="OO439_003292"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6317583.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11258
                     /locus_tag="OO439_003293"
     CDS             10900..>11258
                     /locus_tag="OO439_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6317584.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIY"
BASE COUNT         2843 a         2606 c         3037 g         2772 t
ORIGIN      
        1 ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacga
//
LOCUS       DAJTVE010000126        11251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000126 DAJTVE010000000
VERSION     DAJTVE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..159
                     /locus_tag="OO439_003294"
     CDS             <1..159
                     /locus_tag="OO439_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6317585.1"
                     /translation="AATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVV
                     SIYLSQDI"
     gene            251..2935
                     /locus_tag="OO439_003295"
     CDS             251..2935
                     /locus_tag="OO439_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6317586.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2932..4020
                     /locus_tag="OO439_003296"
     CDS             2932..4020
                     /locus_tag="OO439_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6317587.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4103..5400)
                     /gene="exuT"
                     /locus_tag="OO439_003297"
                     /pseudo
     CDS             complement(4103..5400)
                     /gene="exuT"
                     /locus_tag="OO439_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5883..7295
                     /gene="uxaC"
                     /locus_tag="OO439_003298"
     CDS             5883..7295
                     /gene="uxaC"
                     /locus_tag="OO439_003298"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6317588.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7310..8797
                     /gene="uxaA"
                     /locus_tag="OO439_003299"
     CDS             7310..8797
                     /gene="uxaA"
                     /locus_tag="OO439_003299"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6317589.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8880..9431
                     /locus_tag="OO439_003300"
     CDS             8880..9431
                     /locus_tag="OO439_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6317590.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9436..10680)
                     /gene="sstT"
                     /locus_tag="OO439_003301"
     CDS             complement(9436..10680)
                     /gene="sstT"
                     /locus_tag="OO439_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6317591.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2953 a         2764 c         2916 g         2618 t
ORIGIN      
        1 gcggcaacca cttttaccgc aaccaaaatc tttgcttccg atgcgttaac taacggttct
       61 ctggctaaaa acctgatgtt tgctcaaacc accaaaggtg ttctggagac aggcatctac
      121 cgtggtgtag taagcattta tctgtctcag gatatctaat ccagataaca gaattaacac
      181 gtaacaaagg gaggggtagc cctcccttat tttaactgtt ttagtatttg ttatggattt
      241 caggattgcc atggataaaa aattactggc tcttttgatc ctggcgagtc tcagcccggc
      301 agaggcgaca ttaaccgaaa ttcccgcagg gtttgaggtt attgctcagg gacagcagga
      361 gtatatcgag gtttattttt cagggaaaag tctcggtaaa tattatgcaa tggttaatct
      421 tgataccgta acatttcttg atccagcaag tctatataac aagctggaac tggatgtaga
      481 cgatcagaaa atcgcgcata tagtgaaaga aaaattatcg cagccgctag ctcaccacgg
      541 tgaattggct tgcggttatg tacgtactga ctcagggtgt ggttttctga ataccgatac
      601 gctggaaata atctataatg atgaagaaag ttcggcaacg ttgtttatta atccgcaatg
      661 gaattcagct ttcgatgcga agtcattata tttaaatcca gacaaaaata ctgttaatgc
      721 ttttatacat cagcaagaca tcaatgttct ggcacaggat gattaccaat cgttgtctat
      781 tcagggaaac ggtgcgctgg gaataacaga aaatagttat attggtgcac actggaattt
      841 caacggttat gatgcagatg atgtcagtga cagtaatgct gacgtcagcg atctctatta
      901 tcgttatgat tttttacgtc gttattatgt gcaggcgggt cgcatggaca accgcacact
      961 atttaatgca caaggcggga actttacctt taactttctg ccactcggtg caattgatgg
     1021 gatgcgtatc gggtcgacac tgagctattt aaaccaggcg caaagccagc aaggaacccc
     1081 agtaatggtt ctgctttcgc gcaattctcg tgttgacgct tatcgtaatg agcaactttt
     1141 gggatcgttt tatctcaata gtggttcgca atttattgat accagttcct ttccgccagg
     1201 cagctacagc gtggcattaa aagtctacga aaataaccaa ctcacccgca ccgagcttgt
     1261 gccgtttacc aaaaccggcg gtctgactga cggaaatgcg caatggttct tacaggcagg
     1321 taaaactaca tcacaggttt ctgatgatga aagttcagct tatcagctag gagtacgcct
     1381 gccattacat ccgcaatatg agctctacgc agggctggcg aatgccgatg atgtgagtgc
     1441 tttcgagtta ggtaataact ggacggcaga tttaggcggg gtggggaatc ttgcaatcag
     1501 cgccagcgtg ttccgtaacg atgacggcgg caaaggtgat atgcaacagg ccaactggag
     1561 taatccggga tggccgacgt tgggctttta tcggaccaac tctgacggcg atgcttgtac
     1621 aaccgacagc agagagagct ataacgcctt aagctgttat gaaagtattt ccgcgacggt
     1681 ttcacagaat tttgtcggct ggaatatgat gctgggttat acccgcacac aaaataacac
     1741 tgatgatagt ttgcgttggg ataaacagca gagctttgaa aataactatc ttcgccagac
     1801 aacagcgcaa agtatctctg aaactgtaca acttagcgct tcccgcgctt ttgtgatgcg
     1861 tgactggatt ttgagtacgt cagtcggtgt tttccatcgt aatgacaacg gtggcgataa
     1921 cgacgacaac ggcttgtact tatcgttttc gttatctgac acgccaacga tggatagcaa
     1981 taacaacagc cattcaacaa atgtgtctac ggattatcgt tatagcgaac aggatggcga
     2041 tcaaacgtca tggcagttat cgcatacctt ttataacgat tcattcagcc ataaagaact
     2101 tggcgtaacc gtcggaggcc tgaacaccga taccataaac agcgcggtta acgggcgttg
     2161 ggatgggcaa tacggaaatg tctacgctac cgtatctgat agttatgacc gtaagaatca
     2221 tgatcatctt tcggccttta cgggcactta tagctctacg ctggctgtca gttgctatgg
     2281 cgttaatttg ggagccagtg gtacagacga tttgctgggt gcggtactgg tggatgtgaa
     2341 aggcttctct gaacaggatg aagagagtca ggatctgcaa ctcgaagcgc gggtggcagg
     2401 cagccgaact ttgcagcttg gtcaaagtga cagtgtgttg ttcccttatc ctggatttca
     2461 gtctggtttt gttgaggtta acgacagtag ccagggcaat cagcagggga caacaaacat
     2521 cattaacggt gcagggaatc gtgaattaat gttgttgcct ggcaagctgc gctatcgcga
     2581 agtgtctgcc agctttaact acaactatat cggtcgttta ttactgccgg cagcggtgaa
     2641 aaaattcccg attgtggggc tgaacagcgc catgttactg gtagctgaag atggaggatt
     2701 tacacttgaa attaacggca gcgaaaaaga gctctatctg ctttccgggc agcaattcct
     2761 taagtgtcca ctgagtgttg taaagaaacg cgccagcatt cgttacagcg gagatgttac
     2821 ttgtagtgtg gtgacttatt cacaattacc ggagtcgatt caggttcagg cacagttgaa
     2881 acagcctaaa ttacgtggaa acgttcagac ggcgcaaagg gaggttgcac catgagaaat
     2941 cgattgattg cggcgatatt gggcttgttt ggcacgctca ctggcgttca ggcagctcct
     3001 gacgtgacca gtgaaattac gtatgatttg gcatctggta gagcggatta ttacttctgg
     3061 aaggatgaag cgtcggcagg aaataatgga tatatgtggt atgaatgttc gtatcctgac
     3121 ctccaacaaa cctgtacagc taatggaaat atatcgacag tacaaatcta tttaactgaa
     3181 caacgcagtg ggatgcgttg gccggtaaaa ctcaaaggat ttaaaacagc cattgtaagt
     3241 agcgatgaag cgccgccagg atgcaagggg ggcaaagggc ttcagacgaa tcttaaggat
     3301 tctaatagat cttcatgtac agaagatggt caacattatt atatatacga tacaaagttt
     3361 cttacgctct accttgagca gacagagatg aagaatttgc cgattggtgg cgtctggaag
     3421 gggaaagtta aattacattc gaacagcccg gcccaggact atttcgcaaa tattaccctg
     3481 aatacgctcg accccaacca tattgacgtg ttcttcccgg agttcgccca cgccacgcca
     3541 agggtgcagt tagacttgca tccaacagga agcgttaacg gcagcaacta cgcgcaagat
     3601 ctgaccatgt tggacatgtg cctgtacgat ggttttaacg gtaatgccat cagttatgaa
     3661 atcatgctca aagatgaagg gcgaccagct gcagggcgca gagacggtga cttctctatc
     3721 tatcgtcaag gagggaccac caccgacgag ggagaacgca ttgattaccg ggtcaaaatg
     3781 tacaacccgg aaaccggtgg gcaaattgat gtgcgcaata atgaaaatat ggtctggaac
     3841 agcattaacc tgaaacgtgt gcgtccggtg gtactgcccg gtattcgcta tgccgtgatg
     3901 tgtgtgccga cacccttgac tctggcagtc gataaattca gcgtgatgga taaacaggcc
     3961 gggtactaca tgggcaaatt gtcagtaatc tttacgcctt ccttgccaac catcaattga
     4021 ccccgcagac cagaaaaaac taaacaacga agccgcctga taaggcggct tcgtcattac
     4081 aacttacgtt aaacgttaaa cgttaatgtt gcggtgcggg atcgttatgt gtttcctgcg
     4141 ccacctcaat cgccggtttg ttctgcaata cggtccagat aaccagcgca cccagcaggt
     4201 caaatactgc cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga
     4261 caaccagcgc aaacagcgta cttgccagcc atgcggacat cccggttaaa ccgttcgccg
     4321 tcgccacttc gttacgaccg aacacgtcag aagagagcgt aatcagcgcg ccagacagtg
     4381 cctggtgggc aaaaccaccg atacacagca gcataattgc gacatacggg ttggtgaaca
     4441 gaccgatcat acccgggcca atcatcagca cagcacccag cgttacgacc atcttacggg
     4501 aaacgatcag gttcacacca aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga
     4561 tacaaccgag gtcagcaaac agcatcggca tccaggcgaa catcgcgatc tctttcaggt
     4621 taaagccgta aactttaaac atgaacagcg ggatccacgc gttaaaagta ccccaggccg
     4681 gttctgccag gaaacgcggc agtgcgatac cccagaactg acggttacgc aggatctgac
     4741 caacggacat tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca ttaataatat
     4801 agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc
     4861 atgccatcgc ccagataaag ctcaatgcac cggagatgat aaatgccatc tgccagctgt
     4921 gcatcacgat tgccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac
     4981 gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt
     5041 cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag caacagccag
     5101 gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca gcacagcaaa
     5161 cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta ccggttgcat
     5221 gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt
     5281 taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag
     5341 cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat
     5401 gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct
     5461 gaagatgtta gttacgaccg ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat
     5521 gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat
     5581 gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga taacttgtca
     5641 tacaacttta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg acattacctg
     5701 acgacagcga ggccagtact ggcgcggcct gcagcgagat ttatcacttt gagagtaatt
     5761 tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg
     5821 tgataacttt gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga
     5881 aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt
     5941 atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc
     6001 agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggctgaaa ggcgatcact
     6061 acaaatggcg cgctatgcgt accaacggtg tggccgagcg tctgtgtacc ggtgatgcat
     6121 ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt
     6181 tataccactg gacgcacctt gaactgcgtc gtccgtttgg tatcactggc aaattgcttt
     6241 ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact
     6301 tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc
     6361 cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag
     6421 tgctgccgag ctggcgtccg gacaaagcct tcaacatcga acaggcgacc tttaacgact
     6481 acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa
     6541 ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg
     6601 cgctggacgt ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc
     6661 gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac
     6721 tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg
     6781 cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact
     6841 ccatcaacga ccgtccgatg gcggaagagt tgtctaagct gctgagcaag cagaacgaag
     6901 aaaacctgct gccgaaaacc attctctact gcctgaaccc gcgcgataac gaagtgctgg
     6961 gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg
     7021 gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc
     7081 tcggtctgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca
     7141 cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg
     7201 gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca
     7261 atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat
     7321 caagatccat gcgctggata acgtcgcggt cgctttagca gatttggctg aaggcacaga
     7381 agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt
     7441 tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc cgattggtta
     7501 tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct
     7561 gagcgatctg gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc
     7621 agatcgtgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct
     7681 gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc agaaccgttt
     7741 cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca gccacaccta
     7801 cggctgctca caactgggcg acgatcacat caacatccgc accatgctgc aaaacatggt
     7861 gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa acaaccaggt
     7921 tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg
     7981 ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt
     8041 gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg
     8101 cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga
     8161 ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc
     8221 agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat
     8281 ggtcaacgac tttaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc
     8341 accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca
     8401 gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc
     8461 tgggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg
     8521 tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt
     8581 gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggatcga
     8641 ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat
     8701 cgacaccatc gttgagtttg ccaacggtaa gcaaacttgc aacgagcgta acgacttccg
     8761 cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa
     8821 aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgataa ggatttatca
     8881 tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa
     8941 catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat agcgccgtta
     9001 ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg
     9061 ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg
     9121 tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta
     9181 tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc atgcgctgcg
     9241 tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg
     9301 gcacaatgat tgagggtagt tttctgctta tgaatggctt gaatatcatt cgtttctggc
     9361 ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc gccgtagacg
     9421 aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg
     9481 gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc
     9541 ctgcaatacg ccgatgataa agccgacggc aaccacctgc atggcgatat cgttcgaaat
     9601 accgaacata ttacaggcca gcgggatcag cagcagagaa ccccccgcca cgccggatgc
     9661 gccacaggca cacagagaag ccaccacgct caacagcagc gccgtgggca gatcgaccgg
     9721 gatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc
     9781 catattgatg gtggcaccca gcggaataga aacggaatag gtatcgcgat ccagattcag
     9841 cttttcacac agtgccatat tcaccggaat gttcgccgca gagctgcggg tgaagaaggc
     9901 atacacgccg ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt
     9961 ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac
    10021 gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga
    10081 aaccagacca aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca taaaggtgac
    10141 ggcattcgac atatcgttga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa
    10201 accgaggcca attgcccaca ccaggatccc gatgtagtta cctttcagca gcgcgtcgat
    10261 ggggttggaa accatgctca ttaccagccc gcgcatcact tctacaatgc ctgacggtgg
    10321 cgaaatatca cccgcgctac tggataagtg cagggtagaa gggaaggcga agctgaaaac
    10381 gactgcggcc agagcagcag agaaggtacc cagcagatag aggaacaaaa tagggcggat
    10441 attggttttc tgcccatgct ggtggttagc aatagatgcc atcaccagca tcaacaccag
    10501 gatgggggca acggctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc
    10561 cgccgcgggt tttgatatcc atgccagaag aatccccaga acaaggccga ccaggatttg
    10621 ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat
    10681 ttttcgatcc tttcattgtg ttatcgcaca tccctggtgc gttctgtcga cgcatctttc
    10741 aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga
    10801 aaaatgctgg attttaaaga gaagtcatat tttgaaactt aatggttaca tttgtttgac
    10861 ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt
    10921 tggaatatga gaacggttgc gctcctttaa tgtgctatgc catataaagt cactggcgca
    10981 gggagcgcgc agggggcggc caatcgccgc cgccccctgc acccccaggc tctggcgaac
    11041 aaaatcgccg ctgcgcgatt ccctcagctt atcccttgcg gctaccgggt cgggcgcaag
    11101 ggaacgtccc tgtaagatgc gccctcgccg cgcatccatg cgcgtcgccc cggcctccag
    11161 ggaacgcttc ggcgattttg acgccaccaa cacccgtgcg gtctttttgt aaagagctac
    11221 aagccgatta cataattaat agctcacctt t
//
LOCUS       DAJTVE010000127        11232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000127 DAJTVE010000000
VERSION     DAJTVE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..309
                     /locus_tag="OO439_003302"
     CDS             <1..309
                     /locus_tag="OO439_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6317592.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OO439_003303"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OO439_003303"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6317593.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO439_003304"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO439_003304"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6317594.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OO439_003305"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OO439_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6317595.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO439_003306"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO439_003306"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6317596.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO439_003307"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO439_003307"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6317597.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO439_003308"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO439_003308"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6317598.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO439_003309"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO439_003309"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6317599.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OO439_003310"
     CDS             complement(8117..9763)
                     /locus_tag="OO439_003310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6317600.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OO439_003311"
     CDS             complement(9766..9816)
                     /locus_tag="OO439_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317601.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO439_003312"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO439_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6317602.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..11232
                     /locus_tag="OO439_003313"
                     /pseudo
     CDS             10354..11232
                     /locus_tag="OO439_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2552 a         3087 c         2946 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcagattcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10861 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10921 tctcagtgat gttgtcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10981 caggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    11041 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
    11101 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11161 tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga
    11221 aatttcgttg gc
//
LOCUS       DAJTVE010000128        11230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000128 DAJTVE010000000
VERSION     DAJTVE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..325)
                     /locus_tag="OO439_003314"
     CDS             complement(<1..325)
                     /locus_tag="OO439_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6317603.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQ"
     gene            complement(448..2094)
                     /gene="fumB"
                     /locus_tag="OO439_003315"
     CDS             complement(448..2094)
                     /gene="fumB"
                     /locus_tag="OO439_003315"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6317604.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2172..3512)
                     /gene="dcuB"
                     /locus_tag="OO439_003316"
     CDS             complement(2172..3512)
                     /gene="dcuB"
                     /locus_tag="OO439_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6317605.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4083..4802)
                     /gene="dcuR"
                     /locus_tag="OO439_003317"
     CDS             complement(4083..4802)
                     /gene="dcuR"
                     /locus_tag="OO439_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6317606.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(4799..6430)
                     /gene="dcuS"
                     /locus_tag="OO439_003318"
     CDS             complement(4799..6430)
                     /gene="dcuS"
                     /locus_tag="OO439_003318"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6317607.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6611..6841
                     /locus_tag="OO439_003319"
     CDS             6611..6841
                     /locus_tag="OO439_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6317608.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            6853..7125
                     /locus_tag="OO439_003320"
     CDS             6853..7125
                     /locus_tag="OO439_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6317609.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7352..7648
                     /gene="ghoS"
                     /locus_tag="OO439_003321"
     CDS             7352..7648
                     /gene="ghoS"
                     /locus_tag="OO439_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6317610.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7676..7849
                     /gene="ghoT"
                     /locus_tag="OO439_003322"
     CDS             7676..7849
                     /gene="ghoT"
                     /locus_tag="OO439_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6317611.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7968..9485)
                     /gene="lysS"
                     /locus_tag="OO439_003323"
     CDS             complement(7968..9485)
                     /gene="lysS"
                     /locus_tag="OO439_003323"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6317612.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(9722..11179)
                     /gene="dtpC"
                     /locus_tag="OO439_003324"
     CDS             complement(9722..11179)
                     /gene="dtpC"
                     /locus_tag="OO439_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6317613.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         2915 a         2690 c         2761 g         2864 t
ORIGIN      
        1 cctggaaaaa atgccccagc ttgtcatacg gattacggct caaccccagc cattcctgca
       61 cctcaaaacc gatggggact ttcgcgtagg tgtattgccc gccgaccatc aggatgatgg
      121 cgtgaaagaa aatgagcgta tagagcagcg gcgttaacgg ataacgttta gcggtggcaa
      181 gcagtagcgg cacgacaata atcaccggtg tcacttccat cagccaggtg agtttgtcat
      241 gggccgaaat gccggtataa atcaggatta acgttagcgc cagtgcgccg gtgttaagaa
      301 ttaacggttt gagtgtgcgg gtcatggtga gttcgcaagt cagggaaaat actgactatt
      361 caccagcaag catgaaatta caatcgcaaa agaggtggcc cagggggatc acctggcagc
      421 atgctgccag gcgctgggcc gaagaggtta cttagtgcag ttcgcgcact gtttgttgac
      481 gatttgctgg aagaagtcgt tacctttgtc atcgaccagg ataaacgccg ggaaatcttc
      541 tacttcgatt ttccagatag cttccatacc cagctccgga taagcgacgc attccagatg
      601 cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg atgctaccga ggtagaagcc
      661 gccgtgttta tgacacgcgt cggtaacctg ctggctgcgg ttgcctttcg ccagcatgat
      721 catgctgccg ccgtgggatt gcagcagatc cacataggag tccatacgac ctgcggtggt
      781 tgggccaagt gaacctgatg gataaccggc aggagttttc gccggacccg cgtagtagat
      841 cgggtgatct ttgatgtact gcggaagttc tttaccggcg tcaatcagct ctttcagctt
      901 ggcgtgtgca atatctcgac ccacaataat ggtgccagtg agcgacaaac gagtggaaac
      961 cggatattgc gaaagctggg cgaggatctc tttcatcggg cggttaaggt cgaccttcac
     1021 cgcttcgcct tcaccggcct gacgcagttc ttgtggaatg tactgacctg gattgtgttc
     1081 cagtttttcg atccagatac cttcgcggtt gattttcgct ttaatgttac ggtcagcgga
     1141 gcaggagacg cccatgccta ccgggcagga tgcgccgtga cgtggcagac ggataacgcg
     1201 aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca agaccgagtt tctgggcctc
     1261 ttccagcagt tcctgttcca gctggacatc gcggaacgcc tgaccatgtt cgttcccttc
     1321 cgtcggcagt tcatcgtaat agtgagcgct tgctaatttg acggttttca ggttggtttc
     1381 cgcagacgta ccgccaatca caaacgcgat atggtacggc gggcaggctg cagtaccgag
     1441 ggtacgcatt ttctcgacga ggaagttttt cagtttgccg ggagtcagca gggctttggt
     1501 ttcctggtag agatacgttt tgttggcaga gccgccgcct ttcgcaacgc aaaggaattt
     1561 gtactcatcg ccatctaccg cgtacaggtc gatttgcgca ggcaggttag tgccggtgtt
     1621 gacctctttg tacatgtcca gcgccgcgtt ttgcgagtag cgcaggttat cttcgatata
     1681 ggtgttatag acgcctttcg acagcgcttc ttcatcaccg ccaccggtcc acacgcgctg
     1741 gcctttttta ccgacgatga tcgcggtgcc ggtatcctgg caggtcggca gcacgccttt
     1801 ggcggcgatt tcggagtttc ttaagaattg cagtgccacg tacttgtcgt tttcgctggc
     1861 ttctggatcg tgaagaatag ccgcaacctg tttctggtgt gccggacgga gcatgaaaga
     1921 agcgtcgtga aaggcttgct gcgccagcag ggtcagggct tctggttcca ctttcaggat
     1981 ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa gtgagtagat agtattcggt
     2041 attgtctttc cccatcggga aaggtgcctg gtagataaag ggtttgtttg acatagcttc
     2101 cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc cgcgtagtca cgggcggcac
     2161 gcaaagtgca tttataagaa cccgtacatc gcggcgaaga tccagccgaa gacgcacgat
     2221 acgctcacac caatcaaccc cggcagaata aagctgtggt tgatgacgaa gcgaccgatg
     2281 tgggtggtgc cggaacggtc aaactgaatc gctgccagat cgctcggata agtcggcagg
     2341 atgtaataac cgtagcaagc cggtgctgaa gccacgatgt atgccggatc aacgccgatc
     2401 gccagcgcga ccggaacaat cgccgccagc gccgcagcct gagagtttac aaacttggaa
     2461 accagcagca gaacaatggc ataggcccac ggatactctt tcaccatttc acccagtacg
     2521 ccctgaattt cagacatatg cgcaccgaac atggtttctg ccatccatgc gataccgtac
     2581 accgccacga tggcgatcat accggaacgg aagacttcgt tttttgagat agacgcggga
     2641 ttggttttgg tcaggataat aatcagcgcc ccggtcagca gcataaacat ctgaataacc
     2701 agtaccatcg acagcggttt gccgccgaag gatggacgca ggtccgaatc agcaccaaga
     2761 agggcgacta cagcgattgc cccgaggaaa atccacattg ccagccagtt gcttttcggc
     2821 agttttttgt ccagcagcgt cgcggtatca ccgtaaacat actcacggtt ttccggtacg
     2881 gagatgaatt tctggaactc ttcgtcttta tccagatctt taccacggaa ccagctgaag
     2941 ataccgatcg ccaggatacc gattaacgtc gatggaatgg tgattgccag caggtcgagg
     3001 aactcaagat ggcgaccatc aaaggtgaca ttacccagca ttgcaaccag agacacgacc
     3061 gcaaccgaca ccggactggc gatgatcccc atctgtgcac cgatagaact tgccgccatc
     3121 ggacgttccg gacggatgtt gttcttaatg gcgacgtcgt agatgatcgg cagaatggtg
     3181 taaaccacat gacccgtacc gcaaagaatg gtcagcgtac aggtcacaaa cggcgcgaca
     3241 attgagacat atttcgggtt gcggcgcagc agcttctcgg caatttgcag catgacatca
     3301 agaccgcccg aagcttgcaa ggtcgccgat gccgccacca ccgcaatgat aaccagcatg
     3361 acatcaaccg gtggtttacc tggctgaagg tggaagacga agaccagaat gaccagaccg
     3421 ataccgccta ataaacccag cgcgatacca ccctttctgg caccataaaa cagacatatc
     3481 agtattatga taagttggat agtaaataac atgtgtgaac cctcgcgata atcctattta
     3541 aatttttgct gaatagatca cagtcacgtt ctgttttgta tgaactgttt tcagagcctg
     3601 actaaatatc cgtttggtcg ttaccggctt tagcaaatac ctcacagtga atattggctg
     3661 attaaagcag cggcaaattt atgcactgtg ataagcggct ttttcagaga gaaagactct
     3721 ctggcgatgg gtttatagtt tgtactgcgc agatgttaat gttttgtttg ctgttatctt
     3781 attgatattt atcggtctat tttttgttct cagggcagat gtcattaggt ttatagatta
     3841 atctgatcta cccatttgtg ggtaaaaata cacataatgc gggtgacata atagttaatt
     3901 aacttttgtt agcgttttga aattaaaaac accgttcacc tgaagagata ttaattttta
     3961 gcgatgatgc agggataatt atatttgatc tggcacaagt tttactgatg aaggatgtaa
     4021 cttgtgccag gggtattttg cattacggta ataattatta cttacagata accagcgatc
     4081 cgttattggc aatattgttt cagtagtgag tagtgttctg cctgaatacg gtaacggtaa
     4141 actggacgcc ccgtgacgcc ataatggata ctggtgaaca agatgtggca gttgaccagc
     4201 cagatgaggt atttacggca ggaaacacgc gaaatgttaa cctcgttggc tagctcgtcg
     4261 gttgaaaatt catagtcctg atgcgcgtca atccactggc acagtgtgcg taacgtctgc
     4321 ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt tggagctgct gccgtggatt
     4381 agctgatcaa gctcggcctg gtcataatac tgatgttttt ccagcgccat tttcttttgc
     4441 cgccagtcgg tgagcgcctc ttcaaagcgg gaagcctgga agggtttgat caggtaatcc
     4501 acgacaccgt aatgcagcga atctttaatg gttgccgcat cggctgcgga ggagatgaca
     4561 atcacatcac ttttgcaacg cgcgttatgc aggacaggca gtaaatcgag cccgttctct
     4621 ttttgcatat agatatcgag caatatcagg tcgataggcg tatcgctatt gaagataatc
     4681 tctttggctt tctccagcgt cgaggctgtt ccacagcatt gaaagcctgg gatttgtgct
     4741 acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat cgataattaa tacattgatc
     4801 atctgttcga cctctccccg tcccagggta tctggacaaa aaattgtgtg aaaatcccgg
     4861 gttccgattc cacggcgatg ctgccgccga gattttctac ctgttgtttg acaagtgcta
     4921 aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt gtcaaaaatg tgatcgattt
     4981 tgtcgggtgc gatccccggt ccatcatcgt taacttcgca gtgcagccag ccgtgacggt
     5041 agtgcaatgt tacgctaatt tcgccaccgg gttccggccc taatgcttcc agcgcgtttt
     5101 ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg gtcctcactg ccgctgtctg
     5161 gcagctggct ttcactgttt aaaatcagcg tatggcctaa atcggtcgcg cgattaatct
     5221 tgctgattaa aaaaccagcg ataaccggag atttgatctt acccagcaga gagccaatct
     5281 cttcctgata gttattggct gtttttagaa tgtaatcttc caactgctta taactcttca
     5341 gatgcaataa tccgagaatc acgtgcaatt tattcataaa ttcgtgggat cgttcacgaa
     5401 gtgcgtcggc atagttgacc agaccgtcga gtcgctgcat cagtttacgt acttcggttt
     5461 tgtccctgaa ggttgaaatg gcaccgatga taacgccatt actgcgcacc ggaacggtgt
     5521 tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc gcggcgcggc gtaccgtcgc
     5581 gtaacacttc cgagacatct accacctgtg accatgagtg gctgagcgtc gacagtttct
     5641 catcgtcctg cgacttacgg taattcagca attcttgtgc ggcatcgttg atcagcgtga
     5701 cctcgccgcg atcgtccacg gcaacgacgc cttctttgat agactgcaac atggcctggc
     5761 gttgctcaaa cagcgtggag atttcgtagg gttccaggcc gaaaaggatt tttttcagta
     5821 ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat gccaaataat accgaccaga
     5881 taatgctcca gcgactgtca ttgatttgtt gggtcacgcg gcttaactca aggccgatcg
     5941 ccaccacgcc aatttgttta tgattttcat cgtagatggg ggtaaatacg cgtaaagcct
     6001 gcgccagaaa accgcgattg atagcgacat tttctttgcc attcagcgct ttaaggatgt
     6061 catcaccttt aaatggctga ccaatacgct gggcttcagg atgcgagtag cgaagacttt
     6121 gcatatcggt aacgacaata aacagcagat cgttgcgttt gcgcacggct tccgcgatgg
     6181 cctggatgcc gctctcctgc ggttttttct gcaagccctg acggatttcc ggcgagttgg
     6241 cgagggtacg cgccactgcc agtgccttgt tggctagccc gtctcgcgtc atatcactga
     6301 tttgcgagaa gtaaatcaga tgcaccacca atagcaccga gaacagtacc gcactgacca
     6361 ttaagatcac tgtggtactc aatttcatcg gacgtttgcg taacatgcgg tagggcaatg
     6421 aatgtctcat cagcttcctt gtgtgacaaa tttcttaaac attatctctg atgaggtggg
     6481 taattcaaag ggagtaagaa tgattggcta tataggggaa gagattctgg caacggaaac
     6541 tgccagtgct gtatgaagat tccagggcta tgcttatagc gataatcata ctgatgagag
     6601 agggaaagtc atggatcagg cgctactgga cgagggttat cgctgttata ccggcgaaaa
     6661 gatcgatgtc tatttcaaca ctgcgatatg tcagcattct ggcaattgcg tacgtggcaa
     6721 cggcaagtta tttaatctca aacgaaagcc gtggatcatg ccggatgaag tcgacgtcgc
     6781 cactgtggtt aaagtgattg atacgtgccc gagcggcgcg ctgaaatacc gtcataaata
     6841 agcgagggta aaatggaaat acgcgaaggc cacaataaat tttacattaa tgacgaacaa
     6901 ggcaagcaaa tcgctgaaat tgtctttgtg ccgactggag agaatttagc gattatcgaa
     6961 cataccgatg tcgatgaaag cctgaaaggg caggggattg gtaaacagct ggttgcaaaa
     7021 gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc cactatgccc atttgcgaaa
     7081 cacgaatttg ataaaacgcg ggagtatgat gatattcgca gttgatggga gagtacagag
     7141 tcacgatatt tttcatgctc tccgcggagt gatgcaggag agcatctgaa gggtaggggg
     7201 atgcacaaag aatgggcaga gagcgcgttt ttttgtccca agtcatcccc tttactgagc
     7261 aaaaaaaaga atatctccta tatgagaatc atcaatcggg gttatcaagt tttgcgtccc
     7321 cagagcattc aatattgata ggagtcatat tatggaaggt aaaaacaagt tcaatactta
     7381 tgttgtttct tttgattatc catcatctta ttcctcagtg ttcttaaggt taagatcatt
     7441 gatgtatgat atgaatttct cttctatcgt ggctgatgaa tatgggatac cacgacaatt
     7501 gaatgaaaat tccttcgcaa taacgacatc gttagccgca agtgaaatcg aagatttaat
     7561 caggcttaaa tgcttagact taccggatat tgattttgac ctcaacatta tgacagttga
     7621 tgactatttc cgtcagtttt acaagtagcc aggaaggata agaagaacat aaaaaatggc
     7681 actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct ttgtcattat
     7741 ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc tggtcggaat
     7801 aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg agctgtagtt
     7861 gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc tcaaaactgg atttgcggaa
     7921 cgacgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta tttctgtggg
     7981 cgcatcgccg ggaagagaat aacgtcgcga atagtatgac tgtttgtaaa cagcataatc
     8041 attcggtcga taccaatacc cagaccagcg gttggcggca gaccatattc cagcgcagtt
     8101 acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cagctttagc gttaacctgt
     8161 tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc attaccgatt
     8221 tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ccgggttaac atcattacgg
     8281 cgcgccagcg gtgacacttc tgccggatat tccgtaataa aggttggctg aatcagatgt
     8341 gcttctgcca cttcatcaaa gatctccgtg ataatacgtc ccagccccca gcttttctct
     8401 accgtaattc cgatagattc agctaatgct ttagcagcat caaaattatc caggtcggcc
     8461 atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt ttcaaacggt
     8521 ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtacccag aacctcttgt
     8581 gccagggtgc ggaacagtga ctctgtcagt tcaatcaaat cgtggtaatc cgcatacgcc
     8641 atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc ttcattacgg
     8701 aagttacggt tgatttcgaa tacccgttca aaaccgccta caaccagacg tttcagatac
     8761 agctccggcg cgatacgcag atacatatct aaatccagag cattatgatg ggtaataaac
     8821 gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac ttccataaag
     8881 ccgcgcgcga ccatgaactg acggatagcg gccagaattt ttgaacggac aacaaacgtt
     8941 tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg gacttcctga
     9001 tcctgcagac catggaattt atctggtaaa ggacgtagtg ctttagtcag caggcgcaac
     9061 tcagtacagt gaatggaaag ctcacccgtt tgcgtcttaa acagcgtacc gcgggcaccg
     9121 ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc tggcaggcta
     9181 tctcttgcaa cgtacagttg aatacggcca ccgacatcct gcaacgttac aaaggaggct
     9241 ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat gtttaaggat
     9301 tccagttcct ggttattctt cgcatcaaac tcttcgtgca actggtcaga ggtatggtcg
     9361 cggcgaaaat cattgggaaa cgccacacct tgctgacgta gtgccgccag tttttcgcgg
     9421 cgatttctca gttcatcgtt aaaatcaata gcctcattgg ctccccgtgt ttcttgttca
     9481 gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag acagatcggt
     9541 acactgtatt atctgcctcg actattaata actcagttta tcttatcgaa actaacgctt
     9601 acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac ttattaatct
     9661 cgatttacta gtgtgattaa ctgaaagtta atttattcat aaccaattgt ttttacgaca
     9721 gttaatcgtt gctctcctgt atcatattcg tcggcgtgct aaacagaaaa cgggtagcaa
     9781 aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatc tgagaaaaga
     9841 atcgctggta agctgcaatt accataccgc taatttgcga ctctgtcgtc tgctgtgcca
     9901 cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata ccggttaata
     9961 cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata aagagttctg
    10021 caaagcccat tagcgccaac ccggatatca tcacgcccat tgacgcttga ccgtcagccg
    10081 ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca agccatcagc agtaagccaa
    10141 aggcaaactt cagccagacg cgcaatgttg agttgccgcg gctttctggc gacgccagcc
    10201 aggccagtac aaccccagcg agcatcactg caattgcatt caccgactgg aatagtgctg
    10261 taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac aagctgatgg
    10321 tactgccgcc ctgttgtgcc agtacccaga acaacgtccc gacaaacatc aacaatacaa
    10381 tttgccaaag agcacggcgg tgttcgggga atttgatcat catgcgggca atgatttgtg
    10441 cggcaatgag gcaaacgatc gccagcaaat atccggacca gtcgttctcc agcagcaggg
    10501 taaaaaatac tggggctaaa cagagcatca ccactaacca gctccatact ggtaaggcaa
    10561 atttgacgct agtgagcgct tttttatcca tactacgcgt ggactggaaa tgacgatgac
    10621 cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca aagccaacat
    10681 gccatccata ccactgagca gccaggccgc aggcgattgg ggcggcgata gaaccgatat
    10741 tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc tcgtcgtaga
    10801 gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa ataatgattg
    10861 ccagcgccag atacaggcta aaggttgaat ttgtatcaat gcccagcacc acatggccaa
    10921 gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg cggtcggcaa
    10981 gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg aacaggctga
    11041 tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt aaggcacgca
    11101 tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat atcgcgcgcg
    11161 gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg gcaagccact
    11221 tcccttgtac
//
LOCUS       DAJTVE010000129        11152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000129 DAJTVE010000000
VERSION     DAJTVE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(44..385)
                     /gene="bamE"
                     /locus_tag="OO439_003325"
     CDS             complement(44..385)
                     /gene="bamE"
                     /locus_tag="OO439_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6317614.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(534..2195)
                     /gene="recN"
                     /locus_tag="OO439_003326"
     CDS             complement(534..2195)
                     /gene="recN"
                     /locus_tag="OO439_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6317615.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2281..3159)
                     /gene="nadK"
                     /locus_tag="OO439_003327"
     CDS             complement(2281..3159)
                     /gene="nadK"
                     /locus_tag="OO439_003327"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6317616.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3282..3875
                     /gene="grpE"
                     /locus_tag="OO439_003328"
     CDS             3282..3875
                     /gene="grpE"
                     /locus_tag="OO439_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6317617.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3930..5216)
                     /locus_tag="OO439_003329"
     CDS             complement(3930..5216)
                     /locus_tag="OO439_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6317618.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5237..6028)
                     /locus_tag="OO439_003330"
     CDS             complement(5237..6028)
                     /locus_tag="OO439_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6317619.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6195..7556
                     /gene="ffh"
                     /locus_tag="OO439_003331"
     CDS             6195..7556
                     /gene="ffh"
                     /locus_tag="OO439_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6317620.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7805..8053
                     /gene="rpsP"
                     /locus_tag="OO439_003332"
     CDS             7805..8053
                     /gene="rpsP"
                     /locus_tag="OO439_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6317621.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8072..8620
                     /gene="rimM"
                     /locus_tag="OO439_003333"
     CDS             8072..8620
                     /gene="rimM"
                     /locus_tag="OO439_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6317622.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8651..9418
                     /gene="trmD"
                     /locus_tag="OO439_003334"
     CDS             8651..9418
                     /gene="trmD"
                     /locus_tag="OO439_003334"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6317623.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9460..9807
                     /gene="rplS"
                     /locus_tag="OO439_003335"
     CDS             9460..9807
                     /gene="rplS"
                     /locus_tag="OO439_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6317624.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9884..10366)
                     /locus_tag="OO439_003336"
     CDS             complement(9884..10366)
                     /locus_tag="OO439_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6317625.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10382..>11152)
                     /gene="dgcN"
                     /locus_tag="OO439_003337"
     CDS             complement(10382..>11152)
                     /gene="dgcN"
                     /locus_tag="OO439_003337"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6317626.1"
                     /translation="SHFIWFSLAVLTGCILLASGIAITLTRHLHNGLVEALKNITDVV
                     HDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPL
                     TGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKR
                     LAEVGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMT
                     LSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
BASE COUNT         2739 a         2685 c         2982 g         2746 t
ORIGIN      
        1 tcattgagca ccttttttaa cgtctttgag agcaacttta ttattagtta ccactcagcg
       61 caggtttgtt atcaatattg gtcaacacac cgctactgtt aaaggtcagc gtcagcgttt
      121 gctgcgttac accttcatga cctggttgct ggcggaagac atagaaccag gtattcgtac
      181 caaatggatc ggacatcagc ggtgtaccca atgcgtacgc aacttgttgt tgcgtcatgc
      241 caacacgtat tttggatacg tcgttagcgg tcagatagtt cccctggttg atgtcaggac
      301 ggtaaaccac tcgctccaga gtggaacagc ctgcggtcaa catcaatagt actgctgctg
      361 cagcagtcag cgttttacag cgcatagtga tttgattcct tttcgggccc gagcagtacg
      421 tggctcatct gtaatatgcc gatgataata gacctttcat cactttaaaa ccttttgctt
      481 tccggtctta cggcgttttg ctgtttactc taaccgtgaa gcaggaaaaa agtttacgct
      541 gcaagcagtt ctttcgcatt cgctagtgta ttacgtgtga cttcactgcc accaagcagg
      601 cgcgccagct cttgtaaccg cgctttttta ttcagggatt gcatatgcgt ttctgtcatc
      661 gcaccatcgg tttctttgct gacaaaatag tgttgatgac cacatcccgc gacttgtggc
      721 aggtgggtaa cacacatcac ctgagttgat tcgccgagtt gacgcagcag tttgccgaca
      781 actgccgctg ttggaccgct aatccctaca tccacttcat caaaaatcag tgccggggtt
      841 tccattttac gcgccgtgat gacctggatt gccagtgcga tgcgggacaa ttcaccaccg
      901 gatgcgactt tggcaatagg ctgcattggc tgacctgggt tggtggttac ccgaaactca
      961 atacgatcgg caccgtcagc gcccaggtga tgctcgtcaa atttaacatc gatcgtaaac
     1021 tgcccatgcg gcattgagag cgcatgcata ctgtcggtga tcagctgtgc aagttcttct
     1081 gcatattgct ggcgttgttg gtgtaatgcg cgcgcgattt ccagtgcctg ctgatgatgt
     1141 ttcgttaccg ccagcgcaag cgtttcttgt gagtcggcct gatcgtccag ttgctgctgt
     1201 tcttccagta gcgactggta atactgtggc aatgcctcag ggctgacgtg atgtttacgt
     1261 gccagcgaaa tctgttttga gatgcgctgt tcaagttcaa atagtcggtt gggatcgaga
     1321 tccagacgat cgcagtagtg gcgcagttca tcgctggctt cagcaatctg gatggtagct
     1381 tcttccagca tatcaagtac gccggacagt ttgctgtcca tgccaatcaa ttcgctcacc
     1441 agttgtttag ccgtgtaaag ctgactttgc aggtttgcgt cttcaccgtc ggccattaat
     1501 gccaatgcat tctggctggt ggtcagcaat tgaccgctgt tcgccagacg tttgtactct
     1561 tcgtcgattt gttcaaactc tccgggctgc ggattaaatt cgttaagttc ttttaattgg
     1621 tattgcagca gttccgcacg ggcggcgcgt tcctgactta actgttgatg atgcgcgagg
     1681 tcacggcagc tttgatgcca caactgataa cgtgcggtca tttcctgcag tagagaggtt
     1741 tcattggcat agccatcaag caggaatttt tggtgctcag gtttggtgag taattgatga
     1801 gcgtgctgac catggatctg aatcagcaac tgacccagtt cgcgcagttg tgacagagga
     1861 acagctgtac cgttgatgaa accacgggag cgaccatcgc tgctgatcac gcgacgaagc
     1921 aaacattcat gcccgtcttc aagctggttt tcttccagcc agcgcagagc cgctggcgta
     1981 tctttcagag aaaaacgggc gcacaggtca gcgcgagcag cgccggtacg caccatgtcg
     2041 gcttcagcgc gaccaccgag acaaagaccg agggcatcta ttgcaataga tttacccgcg
     2101 ccggtctcgc cagttattac ggtcatgccg ctatgaaaat caatctcaag ctcacgaacg
     2161 atagcaaagt tgctgatggt cagttgtgcc aacatagtcg ctttcctgta tgaaaaacca
     2221 ttactgttat tgtgtacagt ataaactggt tttatataca gtaaagaggc tggcgtaaaa
     2281 ttagaataat ttttttgacc agccgagctt ggtgcttaat gtgttgaaat aactgtaatc
     2341 tttcggatga atcagattca gatggtaatc acagcgacga atcaggacat cttcaccttc
     2401 ctgaatcggc agtgctatct ggctgtcgca actgatttcc aggtcgttac ggcgatgcga
     2461 aaaacgcaga cggatcgtgc tgctgctgtt tatgaccagt ggtcgtgctg acaacgtatg
     2521 cgggaacatg ggcaccaggg taatcgcatc cagagagggg gtcagaatag gaccgcctgc
     2581 agagagggaa taggcggtgg agcctgttgg cgtcgaaata attagtccat cagatcgctg
     2641 agaaaacgca aagatctcgt cgatatacac ttcgaactca atcatatgcg ccactttgcc
     2701 tggatgaagc accacttcat ttatcgcggt gctgatgcgt ttctggcaat cttgctgaca
     2761 gacttgcgct tccagcaaaa aacgtttctc gctgatgtag tggccttcca gcacatcggc
     2821 taactgttgc tgggcgttat cggggtcaag gtcagtcagg aaacccaggt tgccacggtt
     2881 gattccaata actttaatat cgtaacgggc gagtgtgcgt gccgcgccca gcatattacc
     2941 gtcgccacca acgactaccg cgagatcagc tagttgccca atctccgcga gcgtgccagt
     3001 tttcacattc ttcagttgca gttcgtgagc gatttgttgc tcaacgatga cctcgtaacc
     3061 ttttgtgcac agccagcggt agagcatttc atgtgttgtc agtgcagtgg ggtgccgtgg
     3121 gtgtcccaca atgccaatac acttgaaatg attattcatt tttccgaggt ccttgttgcg
     3181 aagattgatg acaatgtgag tgcttccctt gaaaccctga aactgatccc cataataagc
     3241 gaagttagcg agatgaatgc gaaaaaaacg cggagaaatt catgagtagt aaagaacaga
     3301 aaacgcctga ggggcaagcc ccggaagaaa ttatcatgga tcagcacgaa gagattgagg
     3361 cagttgagcc agaagcttct gctgagcagg tggatccgcg cgatgaaaaa attgcgaatc
     3421 tcgaagctca gctggctgaa gcccagaccc gtgaacgtga cggcattttg cgtgtaaaag
     3481 ccgaaatgga aaacctgcgt cgacgtactg aactggatat tgaaaaagcc cacaaattcg
     3541 cgctggagaa attcatcaac gaattgctgc cggtgattga tagcctggat cgtgcgctgg
     3601 aagtggctga taaagctaac ccggatatgt ctgcgatggt tgaaggcatt gagctgacgc
     3661 tgaagtcgat gctggatgtt gtgcgtaagt ttggtgttga agtgatcgcc gaaactaacg
     3721 tgccgctgga cccgaatgtg catcaggcta tcgcaatggt ggaatctgat gacgttgcgc
     3781 caggtaacgt actgggcatt atgcagaagg gttatacgct gaatggtcgt acgattcgtg
     3841 cggcgatggt aactgtagcg aaagcaaaag cgtaatttct gttttcgtaa taattcacgg
     3901 ccctgcatgc gaatgcaggg ccgttttcgt tactccgcca cactctcgcg cagcggtttc
     3961 acaggaaaaa cttttacctg cttaatcata ttgtcctgta cgtcgagaat atctatatcg
     4021 tactcgccaa tacgcacgcg ggtgcctgcg acagggatct cttccagtgc ctcaagaatg
     4081 acgccattaa ccgtgcgggc atcatcttcc ggtagatgcc agttaaaggc tttgttgatt
     4141 tcccgcacgt tggcggtgcc atcgataatc accgaaccgt cgttttgcgg cgtgacctct
     4201 tcggcaagtg ttggcgacat cgacgtagtg aaatcgccga caatctcttc cagaatatct
     4261 tcaaccgtca ccagcccctg aatgtctcca tactcgttga cgaccaggcc gactttcttt
     4321 ttgttgcgct gaaactttac caactgcgtg ctgagcggcg taccttccgg cacaaaatag
     4381 atctcgtccg cggcgcgcag catggtttct ttggtgaact cttttttctc cgacatcaac
     4441 cgccaggctt cacgtactcg cagcatactg atggcgtcgt ccagcgaatc acggtagagc
     4501 acgatgcgcc cgtgaggtga gtgggagagt tggcgcagaa tcgatttcca gtcatcgttg
     4561 atatcaatac cgataatttc actgcgcggc accatgatgt catcaacggt cattttttcc
     4621 agatcgagca ccgacagcag catatcctga ttgcgacggg aaatttgtga gcgtgattcg
     4681 tgcacgatag tgcgcaactc ttctttgctc aaagagccgc taaccacgat atcggttttg
     4741 atacccatca tgcgcatcag catacgggtg atagcattca gcaaccagac cagcggcatc
     4801 atcaaaattt gcagcggagc cagcagaaaa ctactcggat aagcgacttt ttccgggtac
     4861 agcgcggcaa tggttttcgg caatacctca gcaaatacca gtacgacaaa agtcagcaca
     4921 ccagtcgcaa ttgccacgcc cgcatcgccg tacaaacgca tcccaacaat agtgccgagc
     4981 gcggaggcaa gaatattgac caggttattg ccgattaaca ccaggcttat caggcggtct
     5041 ggcttacgca gcaatttttc gacgcgtttg gccgagcgat taccctgttt cgccatatgt
     5101 cgcagacgat agcggttgag ggtcatcatt ccggtttcgg acccggaaaa ataggctgaa
     5161 atgaccacca tgatgatcag aataatgatc aacgtagtag tagaaatgtg ttccagggga
     5221 aactcctttt ctgggtttag ctgattaact gctggacaat tcggctgccg aagtaggcca
     5281 gtgtcagaat taccgcgccc gcaacgttaa accagacgac gcggcgtcca cgccatcctt
     5341 catggtaatg tccccacagc agcacaatat agacaaacca cgccacgata gagagcacag
     5401 ccttgtcgat attttccatg ctaaataggt tgtgcatgta gaacaggcca gtgcaaagcg
     5461 tgagcgttag cagcaccacg ccaatctgcg tgatgtggaa cattttacgc tcgatactca
     5521 tcaatggcgg catttcctgg ttaaatgcca gcttcttgtt cttcagttgg taatcaatcc
     5581 acgccagttg cagcgcgtac agggcggcga taattagcgt ggcataggaa aagagcgata
     5641 agccaatgtg caccagcatc ccaggcgttg cttccagatg ggtgatgtat tcattgggca
     5701 tgaaggtcgc cagcgccagg ttgataagcg caaaggcata gacaatgggt agcagcaacc
     5761 agccacgatt gcgagaagcc acaatggtca ttaccgtaca gatcatcaaa ctgaccaatg
     5821 aaccaacgtt cagcaggctg aggttttgtc cgctatcacc gtcgggcagg atgcgggctt
     5881 ccagagcgat tgcgtggcag accagcgcaa tgaccgcaga aataatagcc atgcgccgcc
     5941 agccgccgtt tttttgcagc agaccgggaa caatcagcgc aagactgacg gagtaggcga
     6001 caagcgcgag cagagcaaaa acgggcatag tgatgtcgac agtttgagca ataagaaaga
     6061 gaagcagtat agcgtcaggt gaacgctgct ccaaccgttg cataacaaca aagacgcctt
     6121 catgttatac tgcggcaaaa tactgatgat gtgtcgcgat tgcggccaac cgtttccacc
     6181 ccaggcgaga gacaatgttt gataatttaa ccgatcgttt gtcgcgcacg ctgcgcaata
     6241 tcagtggccg tggacgcctc actgaagaca acgtaaaaga tacgctgcgc gaagtgcgca
     6301 tggcgctgct ggaggcggat gtcgctctgc cggtagtgcg tgagtttatc aatcgcgtaa
     6361 aagagaaagc ggttggtcat gaagttaata agagcctgac gccggggcag gagttcgtca
     6421 aaatcgtccg taacgaactg gttgcggcga tgggcgaaga gaaccagacc ctgaacctgg
     6481 cggcgcaacc gcctgcggtc gtgctgatgg cgggcctgca aggtgccggt aaaacaacca
     6541 gcgttggtaa actcggtaag ttcctgcgcg agaagcacaa gaagaaagtg ctggtggttt
     6601 ctgccgacgt ttatcgcccg gcggcaatca aacagcttga gacgctggca gagcaggtgg
     6661 gcgttgattt cttcccttct gatgttggtc agaagccggt agatatcgtt aacgcggcgc
     6721 tgaaagaagc caaactgaaa ttctacgacg tgctgctggt ggataccgct ggtcgtctgc
     6781 acgttgacga agcgatgatg gacgagatca aacaagtcca tgcgtcgatt aacccggttg
     6841 aaaccctgtt tgtggttgac gccatgaccg gtcaggatgc ggccaatacg gcaaaagcat
     6901 tcaatgaagc gttaccgctt accggcgtag tgttgaccaa agtggacggc gatgcccgcg
     6961 gcggtgcggc gctctctatt cgtcacatca ctggcaaacc gatcaagttt ctcggtgttg
     7021 gcgagaaaac tgaggcgctg gagccgttcc atccggaccg catcgcgtcg cgtattctcg
     7081 gcatgggcga cgtactgtcg ctgatcgaag atatcgaaag caaagttgac cgcgcgcagg
     7141 cagagaaatt agccagcaag ctgaaaaaag gcgacggctt cgatctcaac gattttcttg
     7201 agcagctgcg ccagatgaaa aatatgggcg gcatggctag cctgatgggc aagctgccgg
     7261 gcatggggca gatcccggat aatgttaagt cgcagatgga cgataaagtg ctggtgcgta
     7321 tggaagccat catcaactcg atgacgatga aagagcgcgc taagccagaa atcatcaaag
     7381 gttcgcgtaa gcgtcgtatc gctgccggtt gcggtatgca ggtgcaggac gttaaccgcc
     7441 ttctgaagca gttcgacgac atgcagcgca tgatgaagaa aatgaagaag ggcggaatgg
     7501 cgaagatgat gagaagcatg aagggtatga tgcccccagg cttccctggt cgctaatcgg
     7561 cctggcttgt ttgccatttt ggcaccggga tgtgctcgtc atcctcacgt actgcgtgta
     7621 cgctccggtg actgcgcgca ctccggcacc aaactggctg caccgccgac ggccttttac
     7681 agcagagtta gcaactgttg attgcaattc ccgcaaaaat gagtaaaatt ttcgggcttt
     7741 taatatgaca ccggactccg ttcctcgatg gggtccggtt gttttattca cacaagagga
     7801 tgttatggta actattcgtt tagcacgtca cggcgctaaa aagcgtccgt tctaccaggt
     7861 tgttgtcgct gacagccgta atgcacgcaa cggtcgcttc atcgagcgcg ttggtttctt
     7921 caacccaatc gctagcgaaa aagaagaagg cactcgcctg gatctggatc gcatcgctca
     7981 ctgggttggc cagggcgcaa ctatttctga tcgcgttgct gcgctgatca aagaagtaaa
     8041 caaagcagct taatctgtca cggtggtcat gatgagcaaa caactcaccg cgcaagcacc
     8101 tgttgatccc atcgttttgg gaaaaatggg ttcgtcttac ggtattcgtg ggtggctcag
     8161 agtgttttct tccaccgaag acgccgaaag catttttgac tatcagccct ggtttatcca
     8221 gaaggcgggt cagtggcagc aagtccagct ggaaagctgg aagcaccaca atcaggacat
     8281 gatcatcaag ctgaaaggcg ttgacgatcg tgatgcggcg aacctgctga cgaattgtga
     8341 aattgtcgtg gattcatcgc agctgcctca gcttgaagag ggggactact actggaaaga
     8401 cctgatgggc tgccaggtag taaccactga aggctacgat ctcggtaaag tcgtcgatat
     8461 gatggaaacc ggatctaatg acgttctcgt cattaaggca aacctgaaag atgcgtttgg
     8521 tatcaaggaa cgtctcgtac cgttcctcga tgggcaggtt atcaagaaag tcgatctcac
     8581 tactcgttca atcgaagtag attgggatcc tggtttttaa accaccggat aaacggtaaa
     8641 agacggcgct atgtggattg gcataattag cctgtttcct gaaatgttcc gcgcaattac
     8701 cgattacggg gtaactggcc gggcagttaa aaatggcctg ctgagcatcc agagttggag
     8761 tcctcgcgac ttcacgcatg accggcaccg taccgtggac gatcgtcctt acggtggcgg
     8821 accggggatg ttaatgatgg tgcaaccctt gcgggacgcc attcatgcag caaaagccgc
     8881 ggcgggtgaa ggcgcaaagg tgatttatct gtcaccacag ggacgcaagc ttgatcaagc
     8941 gggcgtcagc gaactggcaa cgaatcaaaa attgattctg gtgtgcggtc gctacgaagg
     9001 tatagatgag cgcgtgatcc aaaccgaaat tgacgaagaa tggtcaatcg gcgattacgt
     9061 tctcagtggt ggtgagttac cagcaatgac gctgattgac tccgtttccc ggtttattcc
     9121 gggagtactg ggacatgaag cctcggcaac ggaagattcc tttgctgaag gattgctgga
     9181 ttgcccgcac tatacgcggc ctgaggtgtt agaagggatg gaagttccgc cagtgttact
     9241 gtcgggcaac catgccgaga tacgtcgctg gcgtttgaaa cagtcgctgg gccgtacctg
     9301 gcttagaaga cctgaacttc tggaaaacct ggctctgact gaagagcaag caaggttgct
     9361 ggcggagttc aaaacggaac acgcacaaca gcaacataaa catgatggga tggcgtaagc
     9421 ccccgagata tcagtttacc caggataaga gattaaatta tgagcaatat tattaagcaa
     9481 cttgaacaag agcagatgaa gcaggacgta ccttccttcc gtccgggtga taccgtggaa
     9541 gtgaaagtat gggttgttga aggttccaaa aaacgtctgc aggcattcga gggcgtggtt
     9601 atcgctattc gtaaccgcgg tctgcactct gcattcactg ttcgtaaaat ttccaacggc
     9661 gaaggcgttg agcgtgtctt ccagactcac tctccggtag ttgacagcat ttctgtcaaa
     9721 cgtcgtggtg ctgttcgtaa agctaaactg tactacctgc gtgagcgtac tggtaaggct
     9781 gctcgtatca aagagcgtct taactaagat ttcgcttaag cgacatcctg ttaagaaggg
     9841 ctggccaatt ggctggccct ttttttgtct gtttgctcgt gttttaaggg gttgtaatca
     9901 ccactgcgac gcggcggttc tcggcgcggc cctgggcggt cttgttactg gctatgggat
     9961 atttttttcc taaaccctgt gtggtgagat tgctgcgtgg aatttgtcca ccaatagccc
    10021 atgcatcggc cacgacattc gcccgtttca atgataagcc ttcgttgtaa ctgtcttcac
    10081 catagttatc ggtgtgtcca tccatacggg catgtgttag ccctgtcgag gccaatttgg
    10141 ctgccatggt ttggatctgt tgctggcttt ccgggagcaa tttgtagtca ttttttgcga
    10201 acagaatggc atctgataag cccagcgacc agtcgccggc ggattcagta aatccataag
    10261 attgcatagc ggcgacttgc tcaggagtaa actttccctg aggggactgg cagccagtca
    10321 gtattagtga ggtgaaaatc aggggtgcta ctaggtgctt tatcatatcg atatatcctt
    10381 gttatctcac cagcttttcg gcacgctggt gtttggcctg atacatattg tgatcggcaa
    10441 gctcttgtaa tttttccgca gaggcgtgct caatggtcat cgcgtaacca atgcttaatg
    10501 tcatggtggt ctgatggcca ttatgaagat caaacgggag attaaagatt tgtgtcagtg
    10561 ctgagcatat ctgctgcact tcagattctg actgtacatc atagagcacc atagcgaatt
    10621 catcgccgcc caggcggtat gctttatgtc gcagcccgcc aacttcagct aaccgttttg
    10681 cgatttcaat caagactcta tcgcccgtcg catgacccca ggtatcattg atgtatttga
    10741 aattatcgcc atcaagaaat agtaacgccg acgtttttcg ggcatcggaa ttgttcatca
    10801 acgtgttgat gccgctacga aacgctgcgc ggttagccag cccggttaat gggtcatgta
    10861 gcgcggtacg tagaagctgc gcatttttag cctgtaaacg aagctgccac tcttccattt
    10921 catccagcag actgttgaag tcgagagcga agcggtgaaa ctcagcgata cgttcttccg
    10981 aaactcgtcg ggaaaaattg cggttggaac gcacatcatg tacgacatcg gtgatatttt
    11041 tcagtgcttc caccaggcca ttgtgcaaat ggcgggtgag ggtaattgcg atgcctgatg
    11101 ccagcagaat acaaccggtc agtacggcga gcgaaaacca gataaaatgg ct
//
LOCUS       DAJTVE010000130        10999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000130 DAJTVE010000000
VERSION     DAJTVE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..103
                     /locus_tag="OO439_003338"
     ncRNA           <1..103
                     /ncRNA_class="other"
                     /locus_tag="OO439_003338"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            223..564
                     /locus_tag="OO439_003339"
     CDS             223..564
                     /locus_tag="OO439_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6317627.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(555..1481)
                     /locus_tag="OO439_003340"
     CDS             complement(555..1481)
                     /locus_tag="OO439_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317628.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1571..2569
                     /locus_tag="OO439_003341"
     CDS             1571..2569
                     /locus_tag="OO439_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6317629.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2566..2784)
                     /locus_tag="OO439_003342"
     CDS             complement(2566..2784)
                     /locus_tag="OO439_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6317630.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2786..4801)
                     /gene="ligA"
                     /locus_tag="OO439_003343"
     CDS             complement(2786..4801)
                     /gene="ligA"
                     /locus_tag="OO439_003343"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6317631.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4872..5858)
                     /gene="zipA"
                     /locus_tag="OO439_003344"
     CDS             complement(4872..5858)
                     /gene="zipA"
                     /locus_tag="OO439_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6317632.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6088..6849
                     /gene="cysZ"
                     /locus_tag="OO439_003345"
     CDS             6088..6849
                     /gene="cysZ"
                     /locus_tag="OO439_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6317633.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7034..8005
                     /gene="cysK"
                     /locus_tag="OO439_003346"
     CDS             7034..8005
                     /gene="cysK"
                     /locus_tag="OO439_003346"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6317634.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGRLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8389..8646
                     /gene="ptsH"
                     /locus_tag="OO439_003347"
     CDS             8389..8646
                     /gene="ptsH"
                     /locus_tag="OO439_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6317635.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8691..10418
                     /gene="ptsI"
                     /locus_tag="OO439_003348"
     CDS             8691..10418
                     /gene="ptsI"
                     /locus_tag="OO439_003348"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6317636.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10459..10968
                     /gene="crr"
                     /locus_tag="OO439_003349"
     CDS             10459..10968
                     /gene="crr"
                     /locus_tag="OO439_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6317637.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2676 a         2839 c         2737 g         2747 t
ORIGIN      
        1 tcgaacctgc agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg
       61 gcccgaaggg cgagcgaagc gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct
      121 aaaggcgctt ttttgctatc tgcgatttgt aagattgatt tttcttctca agttccttgt
      181 tcttctgccg atattacctc gtacagtgac gaggactgaa caatggccac tatctcttca
      241 acttcaattc cttccataca aacacagagt agtaacaggg catcacaagg tagcgatgtt
      301 gcttcaaaaa tcgcgcggat aagtcagcaa atcatcaaac tcactcaaca aattaaagag
      361 attgtagaca ctagcggcag tgctgaagac aaacaaaaac aggccgaatt gattcaacag
      421 caaatcaccc tactggaaac acaactggcg cagttgcaga gacagcaggc agaaaaggcg
      481 caggagaaag agcagcgttt gtctctgaat gtatcccttc taaatcctgt tgaaaatacg
      541 actcatatcg atatctatat ctgatacaac ggagcccctt cccgaccagc cattagcgca
      601 ttgccctggt ctcgtaccac gtcccataac gcttcagcgg cggtggaaag tgaacggttc
      661 ttcctgcgca ccagcatcaa ctggcgttca acaaccggcg taatgcgctt taccaccagt
      721 gggctacctt caggcaatgg tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg
      781 gctaccatcg ggaacagcgt cgccggatgt ccaatctcct gcacaatatt cgcctgaata
      841 ccattgcgcg ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac
      901 accaatttcg ctccctgtaa tgcctgccag ggtacataat cttccacggc caaagcgcta
      961 tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg cagatcgccc
     1021 actgggcctg gatcgatgac gatgcaaaaa tcgacatctc cctggcgaat actttccatc
     1081 acccactgct gcggacgatc gtgcaggaca aactgaatat ctggatagcg gcgatggctt
     1141 tcagcaatgc attgcggaat aagatgcgcg gaaatggtct ggctggcagc gacccgaact
     1201 tttccgctca gttgttgccc catacgcccg gtatcgcgca acgtgctgtt aagttcatcc
     1261 agcagtcgct caagacgcaa agccaactgc tgccctgcat ctgtaagcac cacttcacgc
     1321 gtggttctgt ccagcaggcg aacaccagta tgattttcca gctccttcac actgtgactt
     1381 actgccgact ggctcaagcc gatacgctct cctgcacgac taaaactttt ctcctgcgct
     1441 actgtgacga aaacctttaa ttgttttaaa gaataattca tctgttttct tcatgaatgg
     1501 atgcaataaa tcaattttat ttctcaaact ggaagaagca caatagaacc atcgatcatc
     1561 tggagtcttt atgaaacttt ttcgtatcct cgatcctttc accttaaccc tgatcacggt
     1621 ggtgttgctg gcctctttct ttccggccag aggcgatttc gtccccttct ttgaaaatct
     1681 gaccaccgca gctattgccc tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc
     1741 gattattgct ggcggtggtc actggcgact gcatttgtgg gtaatgtgca gcaccttcgt
     1801 gctgtttccg attctgggtg tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat
     1861 gctctactcc ggttttctct acttgtgcat tctcccggct accgtgcagt ctgcaatcgc
     1921 cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag
     1981 cctgctgggg attttccttt caccattgct agttggtctg gtgatgaatg ttcacggtgc
     2041 agggggcagc cttgagcagg tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt
     2101 ggggcatctt tcccggccgt ggattggtga ctgggtgtcg cgcaataaaa aatggattgc
     2161 gaaaactgac cagacgtcca ttctgttggt ggtttataca gcgttcagcg aagccgtcgt
     2221 taatggtatc tggcataaag ttggctgggg atcattgctg tttatcgtgg tggtcagctg
     2281 cgttcttctg gctatcgtga ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa
     2341 taaggcagat gaaattacta tcgtcttttg tggttcgaaa aagagtctgg caaatggcat
     2401 cccgatggca aacattctgt tccccacatc ggtgatcggt atgatggtgc tgcccctgat
     2461 gattttccat cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca
     2521 gaccgaacag ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa gcggacgctt
     2581 caggggctgg accagttgcg tcagcccctc ggttttaatc agcagcgtga tttgcattag
     2641 ctcaccgagc ttccctgccg ggaactcatc tttgcgggca aaccacagca gatactcctc
     2701 cggcaggtcg attaagcgac gccctttgta tttgccaaac ggcattatcg tattggctat
     2761 ttcaatcagc tgctcttttt ccatctcagc tacccagcaa acgcagcatt tccgcttcgt
     2821 cgatgacttc aatgcccagt tcctgcgcct tcgccagttt agatcctgca gcttcacccg
     2881 ctatcaccag atcggttttc ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca
     2941 gtcgagcttt agcgtcatca cgcgacatct ggcttaagct gcccgtaagc accacggttt
     3001 taccagcaaa cgggctgtca atctcttccg cgttgataac gatcggcgca ggccagtgaa
     3061 caccttccgc caacagctcg ctgatgacat tgcggttgct ttcttcggca aagaagttgt
     3121 gaacgtggga tgcaacgaca atgccaacat caggcacctt ttgcagctct tcaatcgaag
     3181 cggcttccag cacttccagc gtgccgaaat atgccgccag acctgctgcg gtggcctcgc
     3241 cgacttcacg gatgccaagt gcatagagga agcgagcaaa ggtggtttct ttcgcttttt
     3301 ccagcgcgtt aaccacgttt tgtgccgatt ttggccccat acgctccagt ccggtcagtt
     3361 tgcctgcggt gagtttgaac agatctgccg gagtgtggac atattctttt tcaaccagct
     3421 gatcgatgat tttgtcgccc attccgtcaa catccatcgc acggcgggaa acaaagtgtt
     3481 tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt
     3541 caccttccac acgctcaacg tcagaaccac ataccggaca atgcgtcggg aatacaacct
     3601 cacgggtatc ttccgggcgt tcagaaagca cgacgttaac cacctgcggg atcacgtcgc
     3661 cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg
     3721 cattgtgtaa ggttgcgtta ctcaccagca cgccagcaac atggacaggt tccagacgcg
     3781 caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca
     3841 tctgctcctg cgccgggaat ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc
     3901 caagctgctc ctgctgttcc agtgagttga ccttaatcac cacgccgtcg atatcaaagc
     3961 ccagcgtcgg gcggtcttct tccactttgt gatagaacgc cagcacttct tccgccgatt
     4021 cacaaagcgt tacccgatcg ctgaccggca acccccactt tttaaattgc agtaaacggc
     4081 caagatgagt atccggcagc tcgccacctt ccagaacacc aacgccatag cagaaaaaag
     4141 tgagcggtcg cttcgctgta atacgcggat caagctgacg cagtgaacca gctgccgcat
     4201 tacgtgggtt agcaaaaact ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga
     4261 accccgcctg cggcaggaac acttcaccac gcacttccag acgcgccggg atattctctc
     4321 cgtgcagctt cagcggaatg gcgcgaatag tacgcacatt agacgtgata tcttccccgg
     4381 tggtgccatc gccacgggtc gcggcactga ctaaaacgcc attttcatac agaatactga
     4441 cggcaaggcc atccagcttc agctcacagc accaggtgac tttctcgttg tttttcagac
     4501 ggtcctgcac acgtttgttg aaagcaagaa agctttcttc atcaaaaacg ttatccagtg
     4561 acagcattgg tacttcatgg cgtatctggc tgaaagccgc cagcggcgca gcacctacac
     4621 gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt
     4681 cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc acatgataaa
     4741 gatattcatg atggcgaagc gtcgttcgca gttctgtcag ttgttgttcg attgattcca
     4801 tatcgcacca tcaatgctaa aaacccccga caagcggggg ttcgaagagg agttaatttg
     4861 ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc
     4921 aatttctgcg gagtcatcat acggcgctga tcgtcaagca cgacaccgcc cacttcatcg
     4981 gcaatatgct gcgcagattg cagcatcagc ttgaagttct gcagctcgtc accgtaagac
     5041 ggtacctgca taaagatagt gacacccgga gtagtgaaat ccttcatttc aggatcaaag
     5101 gttcccggtt tcaccatatt cgccaggctg aataacgccg ggccgctgcc atccgggcta
     5161 agatgacgat ggtaaatatt catatcgcca aaaatgaagc ccgcttgttg aatgctgtta
     5221 agaagcagtt caccgtttag ctcgctaccg tgatgcgccg cgacgttcat gataatcacc
     5281 gcttctttgc gcttcggttt atccataact ggagccggtt ccgctacagg ctcaggctgt
     5341 ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc
     5401 acaggctgcg gcgctggcgc gacctgtggc gaaactggct gctgcaacgg ctgttcaggc
     5461 tgcggctgat aggcaggctg ctgcatcggc tgcgctggac gcggagcatg ttgcggcggt
     5521 acctgcgctt caggcggctg ctggatcggt tgacgcggct gcgcagacgc ataaggcggt
     5581 tggtactggt gttgcggcga cggacgagca gcctcatgct cctgagcgtt agccggggca
     5641 tgattcacgc ggtgaacacg aacctcacca acgccctcat catcttcgac atcctcgtca
     5701 taagaatcgt cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg gaacatagaa
     5761 gatcgttctt tacggctggt ccagaaacca tgtaccagta aagcgattat ggcgatcgcg
     5821 ccaacaatga ttaatatcag acgcaaatcc tgcatcatta tattctctgt tgttctaaca
     5881 ccttgccacc acggcaaaca tttactcact aagagtattt gccgattacc tcaagtgcaa
     5941 gtgcactatt aactttcaca gcacaaagat agatgaaatc gtgctttttg ctgttttttc
     6001 gaacatatcc taactgtcca ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg
     6061 attggtcaaa aggagctcat cctgattatg gtttcatcat tcacatctgc cccacgcagc
     6121 ggtttttact attttgcgca aggctggaag ctcgtctcgc aacctgggat tcggcgtttc
     6181 gttattttac cgctgctggt caatattttg ttgatggggg gcgcattctg gtggctcttt
     6241 acacagctcg atgtctggat cccgactctc atgagttacg ttccggactg gctacaatgg
     6301 ctgagttatc tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt tggctatttc
     6361 ttctccacga ttgctaactg gattgccgct ccgtttaacg gtttattggc tgaacaactg
     6421 gaagcacgat tgactggcgc tacaccgcca gataccggga ttttcggtat catgaaagat
     6481 gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt
     6541 gtattactaa ttctttactt catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg
     6601 ttcctgttta gcgcctggat gttagccatc cagtattgcg attacccctt cgataaccac
     6661 aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag
     6721 tttggtgctt taaccagcct gtttacgatg atcccgctgc ttaatctgtt catcatgccc
     6781 gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg
     6841 tggcggtaac aatctaccgg ttattttgta aaccgtttgt gtgaaacagg ggtggcttat
     6901 gccgcccctt attccatctt gcatgtcatt atttcccttc tgtatataga tatgctaaat
     6961 ccttacttcc gcatattctc tgagcgggta tgctacctgt tgtatcccaa tttcatacag
     7021 ttaaggacag gccatgagta agatttttga agataactcg ctgactatcg gtcacacgcc
     7081 gctggttcgc ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa
     7141 ccccagcttc agcgttaagt gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg
     7201 cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc agcggtaata ccgggattgc
     7261 actggcctat gtagctgccg ctcgcggtta caaactcacc ctgaccatgc cagaaaccat
     7321 gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc tgacggaagg
     7381 tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa attgtcgcca gcaatccaga
     7441 gaaatacctg ctgctgcaac aattcagcaa tccggcaaac cctgaaattc acgaaaaaac
     7501 caccggtccg gagatctggg aagataccga cggtcaggtt gatgtattta ttgctggcgt
     7561 tgggactggc ggtaggctga ctggcgtcag ccgctacatt aaaggcacca aaggcaagac
     7621 cgatcttatc tctgtcgccg ttgagccaac cgattctcca gttatcgccc aggcgctggc
     7681 aggtgaagag attaaacctg gcccgcataa aattcagggt attggcgctg gttttatccc
     7741 ggctaacctc gatctcaagc tggtcgataa agtcattggc atcaccaatg aagaagccat
     7801 ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt gcaggtatct cttctggagc
     7861 agctgttgcc gcggcgttga aactacaaga agatgaaagc tttaccaata agaatattgt
     7921 ggttattcta ccatcatcgg gtgagcgtta tttaagcacc gcattgtttg ccgatctctt
     7981 cactgagaaa gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt
     8041 tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct
     8101 gtcggaactg gtatttaacc agactaatta ttttgatgcg cgaaattaat cgttacagga
     8161 aaagccaaag ctgaatcgat tttatgattt ggttcaattc ttcctttaac ggcataatgt
     8221 ttaatgacgt acgaaacgtc agcggtcaac acccgccagc aatggactgt attgcgctct
     8281 tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca ggctaaagtc gaaccgccag
     8341 gctagacttt agttccacaa cactaaacct ataagttggg gaaatacaat gttccagcaa
     8401 gaagttacca ttaccgctcc gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa
     8461 gaagctaagg gcttcacttc tgaaattact gtgacttcca acggcaaaag cgccagcgcg
     8521 aaaagcctgt ttaaactgca gactctgggc ctgactcaag gtaccgttgt gactatctcc
     8581 gcagaaggcg aagacgagca gaaagcggtt gaacatctgg ttaaactgat ggcggaactc
     8641 gagtaatttc ccgggttctt ttaaaaatca gtcacaagta aggtagggtt atgatttcag
     8701 gcattttagc atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa
     8761 ttgtcattga ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc
     8821 tgagcggtcg tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa
     8881 cgttcggtga agaaaaagaa gccatctttg aagggcatat tatgctgctc gaagatgagg
     8941 agctggagca ggaaatcata gccctgatta aagataaaca catgacagct gacgcagctg
     9001 ctcatgaagt tatcgaaggt caggcttctg ccctggaaga gctggatgat gaatacctga
     9061 aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc
     9121 tgaagattat cgacctgagc gccattcagg atgaagtcat tctggttgcc gctgacctga
     9181 cgccgtccga aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg
     9241 gtggccgtac ttcccacacc tctatcatgg cgcgttctct ggaactgcct gctatcgtgg
     9301 gtaccggtag cgtcacctct caggtgaaaa atgacgacta tctgattctg gatgccgtaa
     9361 ataatcaggt ttacgtcaat ccaaccaacg aagttattga taaaatgcgc gctgttcagg
     9421 agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca gctattacgc
     9481 tggacggtca ccaggtagaa gtatgcgcta acattggtac ggttcgtgac gttgaaggtg
     9541 cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc
     9601 gcgacgcgct gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt
     9661 gtggctcgca ggcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat
     9721 acatgaactt cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga
     9781 tggatcgtaa agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg
     9841 gtaaattgcg cattatgttc ccgatgatca tctctgttga agaagtgcgt gcactgcgca
     9901 aagagatcga aatctacaaa caggaactgc gcgacgaagg taaagcgttt gacgagtcaa
     9961 ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca
    10021 aagaagttga tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg
    10081 accgtggtaa tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact
    10141 tgatcaagca agttattgat gcttctcatg ctgaaggcaa atggactggc atgtgtggtg
    10201 agcttgctgg cgatgaacgt gctacacttc tgttgctggg gatgggtctg gacgaattct
    10261 ctatgagcgc catttctatc ccgcgcatta agaagattat ccgtaacacg aacttcgaag
    10321 atgcgaaggt gttagcagag caggctcttg ctcaaccgac aacggacgag ttaatgacgc
    10381 tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt
    10441 actgcttagg agaagatcat gggtttgttc gataaactga aatctctggt ttccgacgac
    10501 aagaaggata ccggaactat tgagatcatt gctccgctct ctggcgagat cgtcaatatc
    10561 gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa
    10621 ccaacgggta acaaaatggt cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc
    10681 aatcacgcgt tctctatcga atctgatagc ggcgttgaac tgttcgtcca cttcggtatc
    10741 gacaccgttg aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg
    10801 aaagttggcg atactgtcat tgaatttgat ctgccgctgc tggaagagaa agccaagtct
    10861 accctgactc cggttgttat ctccaacatg gacgaaatca aagaactgat caaactgtcc
    10921 ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca agaagtaatt cttgccgcag
    10981 tgaaaaatgg cgcccatcg
//
LOCUS       DAJTVE010000131        10924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000131 DAJTVE010000000
VERSION     DAJTVE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..311)
                     /locus_tag="OO439_003350"
     CDS             complement(<1..311)
                     /locus_tag="OO439_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR6317638.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(330..2207)
                     /gene="bglF"
                     /locus_tag="OO439_003351"
     CDS             complement(330..2207)
                     /gene="bglF"
                     /locus_tag="OO439_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6317639.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2341..3177)
                     /gene="bglG"
                     /locus_tag="OO439_003352"
     CDS             complement(2341..3177)
                     /gene="bglG"
                     /locus_tag="OO439_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6317640.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3463..4188)
                     /gene="phoU"
                     /locus_tag="OO439_003353"
     CDS             complement(3463..4188)
                     /gene="phoU"
                     /locus_tag="OO439_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6317641.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4203..4976)
                     /gene="pstB"
                     /locus_tag="OO439_003354"
     CDS             complement(4203..4976)
                     /gene="pstB"
                     /locus_tag="OO439_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6317642.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5067..5957)
                     /gene="pstA"
                     /locus_tag="OO439_003355"
     CDS             complement(5067..5957)
                     /gene="pstA"
                     /locus_tag="OO439_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6317643.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5957..6916)
                     /gene="pstC"
                     /locus_tag="OO439_003356"
     CDS             complement(5957..6916)
                     /gene="pstC"
                     /locus_tag="OO439_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6317644.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7003..8043)
                     /gene="pstS"
                     /locus_tag="OO439_003357"
     CDS             complement(7003..8043)
                     /gene="pstS"
                     /locus_tag="OO439_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6317645.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8291..9364)
                     /locus_tag="OO439_003358"
     CDS             complement(8291..9364)
                     /locus_tag="OO439_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6317646.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9375..>10924)
                     /locus_tag="OO439_003359"
     CDS             complement(9375..>10924)
                     /locus_tag="OO439_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6317647.1"
                     /translation="IKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGFTP
                     DVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFSHD
                     DTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRR
                     SCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSN
                     TARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGT
                     ALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGI
                     LAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVA
                     ISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIA
                     AIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESGLI
                     EQGVTCH"
BASE COUNT         2851 a         2828 c         2478 g         2767 t
ORIGIN      
        1 gctaaccccg cttcattcgg ttcggcttcg tcgccctgag ggaaaattcg cgcccaggca
       61 atggaaatac gcagacaggt gaagcccatc tcggcaaata acgcgatatc ttccgggtaa
      121 cggtgataaa aatcgatggc gacatctttg atattctctt tccccaggat gcgcggttcc
      181 atttttccca taacgccatg aggctgtaaa tctgaggtcg tgctcccttt gccatcttcc
      241 agccaggcac cttccacctg attggcagct gttgcgccac cccaaagaaa tgtttctgga
      301 aatgctttca taattaactc cttttatcgt tagcgaatga tggataacag cggttcacct
      361 gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct attactgatt
      421 aataccggcg tcgtcacatc aaatccggcc tcgcgaatag cagggatatc aaaagaaatc
      481 agccgatcgc ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg
      541 tccagtttta cggtgtcgat accgacatga atcaggatct ccacaccatc atctgactca
      601 atgccaatgg cgtgtaatgt ggcgaacaac gaagcaattc gacccgcaac cggagaacgc
      661 acttcaccaa ccgagggcag aatggcgata cctttaccca acaggccact ggcaaacgtg
      721 gtatcagcga cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca gataccgccc
      781 tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg gggcaccctg cgctggctga
      841 cgtttagcgg tgatgaaatg aagcatcacc gtaccgacaa atgcgcaacc gatggcaatg
      901 acaccgccaa taacgctggc ccagacggtg aaatcaattc ccgttgacgg gatggtttgc
      961 atgaaggtga aaatacttgg caaaccaaag gagtacactt tcgtttgcgc gtagccaatg
     1021 atggtggccc ccaaagcccc actgatacag gcgataacaa aggggtactt acgcggcaga
     1081 ttaacgccat ataccgctgg ttcggtgata ccaaacaggc ccgtcaacgc cgctgatccc
     1141 gccaccactt ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc cgccccgacc
     1201 tgcgccataa tggcgggcat taacagcggg atcatggtgt cgtagcccag cacggtgaag
     1261 ttattgatac acagcggcac caggccccag tgcagtccga acatgacgaa gatttgccag
     1321 aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca gagataaccg
     1381 gcggcaatca gttcgcttat ccaggttgat agcggcccca ccagcagaaa ggtgacgggt
     1441 gtgataacca tcagacatag caatggtgtg aagaaatttt tgattgccga cggtaaccac
     1501 gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat gggaataacc
     1561 gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc cagcgcatcc
     1621 gctttttgcc cgttctcgaa agcagtcaga attaatggat gcactaacgc tccaccaatc
     1681 accatggcag taaatggatt accgccaaag cgtttccccg cggtgtatcc caggattatc
     1741 gggaagaacc aaaacaaggc atcactggcg ctgaataaaa ttaaataagt accactttgt
     1801 tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat cccggttgcc
     1861 gccatcaaac cgatcagagg cgtaaaaata cctgaaataa cataaacaaa gcggtttagc
     1921 agattacctt tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg
     1981 ctgttgaccg ccaggaagac atcggccaca tggttaccta tgaccacctg aaactggcca
     2041 ccgctttcca ccaccataat aataccgggg gtgtttttca gtacctcagc ttgcgctttg
     2101 ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact aacaatgtta
     2161 tctgcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac ttgccctcta
     2221 ccgctttgcg gcaaaactcc aaaaaaaaaa ctgaaaaaaa cggcctgacg tgaatcaagc
     2281 aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag taataatatt
     2341 tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcgggtga
     2401 aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcac attgccatgc
     2461 ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt taattgaagc
     2521 atgttcaaga atacgccagg ataaaaactt cagatgtgta accagtcgct gataactcaa
     2581 gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca tttcgcgcat
     2641 taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg
     2701 catggcaata aagcccactt catctttcgg taactgcacg cccaaccgtt tatcaataat
     2761 ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga tatcccacag
     2821 caacgggttg ggcagcaaca cgttttgctg aaagcgttta atcgcaaact ggcaatggtc
     2881 agttagcgag atataaatac tgtcctgtaa ttttcccaag cgctcctgcg ctaaagagat
     2941 aatacgatca caggttgcca tcacctcaag aggaatatga cttaagagtt cgcttaatcg
     3001 cccgttcagt tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct
     3061 ttcgccagcg catttttgaa agccaattcc tcgccccatg acgacttttt cccgctgttg
     3121 atcatcaata accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc
     3181 aaggaccttt tttataaaca aaaaaaaccg acttcaccag tattctctgg ttatgtcagg
     3241 ttttgcctgc gaatgcagta acaatccagt catttattaa tgttttttat aacgaacatc
     3301 caggttcgga aattaattta attgcgtgca gagaaaatag caatgcgcta ttgataaaaa
     3361 tatgaccatg ctcgcagtta ttaactttgt gtaattttag gaatttataa agttatatat
     3421 aacaaatccc aataattaag ttattgggat ttgtctggtg aattatttgt cgctatcttt
     3481 tcccgccagc agtttatcca gctcatcacc gcctacatga cggaaatcct gccccttcac
     3541 gtagtagaag ataaactcac aaatattctg gcagcggtcg ccaatacgtt cgatagaacg
     3601 cgcgcagaac agcgcagtaa gtacgctcgg aatggtacgc gaatcttcca tcatgtaggt
     3661 catcagttga cgaacaatac cttcgtattc ctgatcgact tttttatctt cacgataaat
     3721 acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg
     3781 gatggtatga cggcccagcg actccagact taccagcaac ggctgatgct gctgggagaa
     3841 tttctccagc gcagtacggc agattttgtc cgccacgtcg ccaatacgct ccagctcggc
     3901 aatggtttta ctgatcacca taaccagtcg caggtcgctc gccgtcggct gacgtttggc
     3961 gataatgcgc acgcaggctt catcgatcgc cacttccatc atgttgacgt tcttgtcgcc
     4021 ttcgatcacg cgcttcgcca gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga
     4081 aagctgctgc tccaccatgc cacccatggt catcacctgc gtgcggatac tttccagttc
     4141 ggcgttgaac tggccggaaa tatgtttatt aagattgaga ctgtccataa cgcactcctg
     4201 aattaaccgt aacgaccggt gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac
     4261 agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg
     4321 gaacaacgcg cagcctgctg catgttgtgg gtaacgatca ccacggtgta atcctgcttc
     4381 agttcggtga tcagctcttc aatacgcccg gtagagatgg ggtcgagcgc cgaacacggt
     4441 tcgtcgagca gcagcacttc cgggcgaatg gcgataccac gcgcaataca cagacgctgt
     4501 tgctgaccac cagagagaga gtaaccgctc tggtgcaatt tatctttggt ttcgttccac
     4561 aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc
     4621 tcaaacagac gaacaccaaa agcgatgttg tcgtagatgg acatcgggaa cggcgtcggt
     4681 ttctggaaca ccatgcccac tttcgcacgc agcagtgcga tatcctgaga gttggtcagg
     4741 atgttgtcgc catcaagcag aatttcacct tctgcgcgct gctccggata cagttcaaac
     4801 attttgttga aggtacgcag cagcgtcgat ttaccgcagc cggacggccc gataaacgcc
     4861 gttacctggt ttttagcgat atccaggttg atgtttttca gggcatggaa tttgccgtag
     4921 tagaagttca aattacgaac ctgaatttta ctcggggcag tttcaaccat actcattgca
     4981 atcttttcct catcgtacct gatgcttttc gctaatcagg cttaccgtag gccgggtaag
     5041 gcgttcacgc cgcatccggc aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc
     5101 gccagaatgt tcagcagcag gacgcacagg gtaatgatca ataccccggc ccaggccagt
     5161 tgttgccatt ccgcaaacgg gctcatcgca aacttaaaga tcgtcaccgg caggttggcg
     5221 atcggctgca tcatgtccgt gctccagaac tggttggaga gcgcggtaaa cagcagcggc
     5281 gcggtttcac cggcaatacg ggcaatcgcc agcaggatac cggtcataat cccggacacc
     5341 gacgctttca gcgtaatcgc ggagatcatc ttccacttcg gtgtacccag cgcataagcc
     5401 gcttcacgca ggctgtccgg caccagtttc agcatgttct cggtggtgcg gataacgatc
     5461 ggcacctgca acagcgccag cgcaattacg cccgcccagc cggagaagtg ctccatctgt
     5521 gccaccacga tggtgtagac aaacagacca accacaatcg acggcacgga gagcagaatg
     5581 tcgttaatga agcgaatcac ttctgccagc caggatttac gaccatattc cgccagataa
     5641 atccccgcca taatgcccag cggcgtaccg aataccgtgg cccacaaaat taacagcccg
     5701 ctacccgcca gggcgttcgc cagaccacca ccttccgtat tgggcggcgg cgtcatttca
     5761 gtgaacagcg ccagcgacat accgtcgata ccgcgagtga tggtggacat taaaatccag
     5821 atcagccaga acaggccgaa ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt
     5881 ttgaggcggc gacgcgcctg cattttgcgg cgagattcag ccagcgccgc agtggtttgc
     5941 atttcaacca tagccattag cgtgccccct cattcttagc caggcgcata atcataaact
     6001 tcgatgcggc gaggacgatg aaggtaatca caaacaggat caggcccagt tccatcagtg
     6061 cggcaacgtg cagaccggat tccgcttccg caaattcgtt cgccaacgca gaggtgatac
     6121 tattgcccgg catgtacagc gaggcgctgt cgagctggta ggtgttaccg atgataaagg
     6181 tcaccgccat ggtttcaccg agcgcgcggc ccagccccag catgatgccg ccgataacac
     6241 catttttggt gaacggaaga acgatacgcc agataacttc ccaggtggtg cagccaatac
     6301 cgtaggccga ctctttcatc atcaccgggg tttgttcgaa cacatcacgc attaccgccg
     6361 caatgtacgg aataatcatg atggcgagga tcacgcctgc cgcgaggata ccgataccaa
     6421 atgcggggcc agagaacagc gcgccaacaa tcgggatatt cgacatgata ttgccgaccg
     6481 gctcctgaaa gtaaacggcg aacagcggcg caaagataaa caggccccac atgccgtaaa
     6541 cgatacttgg aatggctgcc agcagctcaa tggcgatacc cagcgggcgt ttcagccagc
     6601 caggcgcaag ctcagtcagg aacagggcga taccgaaact caccgggacg gcgatcagca
     6661 gcgcgataaa cgaagtcacc aacgtaccgt agatcggcac cagcgccccg tagatatcgt
     6721 tcggtgcatc ccactctttg gtccatagga aagccagacc aaatttctga atgctcggcc
     6781 aggaggagat gatcagagag acaataatgc cacccaacat caatagcaca atcagcgccg
     6841 ccagttttac cagcacgctg aaaattatgt cgcccttttt acccggtggg ttaaaagcag
     6901 gcttggttgc agccataagt tactcttcag ttaaacgcgt ttacaaagtc attttgtaat
     6961 gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt tattagtaca gcggcttacc
     7021 gctactgtct ttaatattgg tcttccacgc agcgcgaacc tgttcaacta cactatccgg
     7081 caggctggcg taatccaggt cgttcgcctg tttagccccg gttttgtacg cccagtcgaa
     7141 gaatttcagc acttctgtgc cttgttctgg tttcttctga tctttgtgga tcagaatgaa
     7201 cgtggtagag gtaataggcc atgcatcttc gcctttctgg ttggtcagat cctgagcgaa
     7261 ggttttgctc cagtctgcac cttttgctgc attagcgaag ttttcttcgg tcggactaac
     7321 cggtttacca tcagcggaga tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc
     7381 atattcaaca taaccaattg cacccggcag acgctgaacg aacgcggcga taccgtcgtt
     7441 acctttaccg cccagaccga tcggccattt tacggtagag ccagtaccaa cgttgttttt
     7501 ccactcttcg ttcactttcg ccaggtagct ggtgaagacg aaggtagtcc cggagccatc
     7561 tgcgcggcgt actacagcaa tgttttgtga aggcagtttc agacccggat tcagtttggc
     7621 gatggtttca tcatcccact tcttgatttt gcccaggtag atgtcgccga gggttttacc
     7681 atccagcacc agttcgccag acttcagccc tggaatgtta accgccagca ccacgccgcc
     7741 aatcacggtc gggaactgga acagaccttc ctgagccagt ttttcgtcag acagcggcga
     7801 gtcagaggca ccaaaatcaa cggtattagc gataatctgt tttacgccac cggaagaacc
     7861 gataccctgg tagttaactt tattaccgat ttctttctgg taagtgtcag cccatttggc
     7921 atacaccggc gcagggaagg ttgcacctgc acctgtcagg cttgcttctg caaacacaga
     7981 gaaagcgctc atcgataagg tcgcggcgac aacagttgcg acggtggtac gcataacttt
     8041 cataatgtct cctgggagga ttcataaagc attgtttgtt ggctacgaga agcaaaatag
     8101 gacaaacagg tgacagttat atgtaaggaa tatgacattt ttatgacaga aaggaattaa
     8161 caaataactt actgatttta aatgtgaaga tgaaaatccc acatgtcatc tttttgtcat
     8221 gatgcattta aaagtgataa agaaatgggt gagatgacaa cgatgtgatg aacaatcgtt
     8281 gtcagaccta ttagcgcaca atctcaacag ttatcgtcgc tgttgcagta aattgccccg
     8341 gggctgggcg agctccagta aggctcgcag gagcagctga aaaagttaca ctacctgatt
     8401 tttcaccata cactgttgat ttacccatgt ctgttggcaa ctcaccaccg ttcactttga
     8461 tttccttatt atttttgtcg taaattttta tccccacgtt agagtttgaa gttacgacca
     8521 ttgaattgtc cgtattgggt tcggtagcat aaaaagacgt actgatatca tcggtattca
     8581 gtatccccag gttttcacac tcaatagtga ctgttttagt aattttatgt gttgttaccg
     8641 cacttccaac aacagatgaa aaatctgcag ctggtatttc tttaaaatca aaataaactg
     8701 gctgtaaatt atttatttcg caactttgcg gagctgtaat atcgccccga atataaacat
     8761 cagccatcgg ctgtgagtaa tccaccgggg tgtcacggct aattgttcca tacaatgtca
     8821 ctatctttgt ttcaggaata atcaattttc ctgcaaatgt cttcttgata taaaaatata
     8881 ctatcgcttt acccccactg gatatactta aaggttcaat gcggttgcaa ttgtactttg
     8941 tattaccaga cgggttgtta gcatgatatt caaaaggtgc attcacatac ccaaccccaa
     9001 gaatatctat agcaatccca acatcaaggt tcggattaag atcataataa ttaatattag
     9061 agtgaacccc agcataagtt aatgcctggt ttatttttga cgtataataa atatagggga
     9121 attctcctac attaccagta tcacaatgac agcgtccagg atatgaggat ccattagtta
     9181 cagtggtatt aaatgtcgtt ccaaccttat tttcgttcgc ggtaataaga tatcccgaga
     9241 aatcaagcgt acggtgaaat acgccattat caggggtaca gataccttta ggagctgcaa
     9301 gtgctacact acttaacgag agcataaaca cagcaaagca ccactgtttg atatacttgt
     9361 tcataaaaat ccttttaatg acatgtcaca ccctgttcaa ttaatccgct ctcactttct
     9421 ggtgtcgaaa gtgaataatt gaagatgcat tttgttgagg cgttttctcc ccaggataac
     9481 gtaacctgtc ctttttctgg tagaccagaa agataaagtt cgccacggtc accgacaatt
     9541 cctgaatctg ccaaacttgc atcttgtatc gcagcaatgg ctccaaaagg taccggttta
     9601 ccatttgcat ttaacactct gaagagaacg cgataaccca catgagtcac aaactccgct
     9661 ttaaccactg caccacgcgt gggaaccatc gtaacgacgg tattatcaag ttctatattc
     9721 gacttcgaga aatagttatc gctgattgct acccgatttt catcataagg gtttagctgt
     9781 gtcttgaccg tatagccgcg ccagtccgtc gcagcaccgc gcatattatc tatttctaac
     9841 ccggcggccc caggtgcttt aacaagagca atagtttcac ctaactcctg acttaacgtt
     9901 accccttcgc tgtgtgccag aattccccct ctgataccat aattcagcct ctgggaattt
     9961 tggctgtagt tatagctgcc gttcaccgtg ccataagagc catcataggt cagggacata
    10021 ttaccgccaa cctcatcatc atgactgtta taggactgca ttaaattcca tgaaaggtta
    10081 tttctttcca gcgccgtacc gctgagtccg agttgatgtg agctaccgga attatcgttt
    10141 tgcgaaacag aataggtggc gtaactatta ccccaccagc ccaataaagg aacagataaa
    10201 gagagcatgt agatctgctc actggtgtta tcactggcat ggttgttccc cgtcccctgt
    10261 ctggcagtat tggaataact ccaggagacg ttccacgaga gacgcttcca gctactgctg
    10321 tagccaaatt gcattaatcg ttcgacatca tcggtatgcc agtattgttg ccggcttaat
    10381 gtcaggtata aattgccata atctctcccc aacgactgcg ttagcgtccc ctccacgcaa
    10441 ctacgtcggt tacctgtttc ccaaaagtct tcagggctgt tgcgccgcga tgcccactca
    10501 ttgagggtat agtagccctc ggtggagtaa cgatagccaa ccaagcgcaa gctagtgtct
    10561 gtgtcgtcaa atcgttttga atagagaaag cgatatgact gacccgtttc tgtatcatca
    10621 tggctaaaat tcgcacgagc atgtgtcaca tcgaaagaaa ttgcgcccaa agcgccaaga
    10681 tctttaccga cacccagagc tgcagaacca taattttcag cagcctgcat acctccatat
    10741 aaagtgatcc cgtgggaaaa accatgaagt atttgcgcct gaaggacgtc cggtgtaaat
    10801 ccgtcttcat cgcgcaattc acccacgctg acatcaacat ctgtctgacc ttcacgttta
    10861 agaatcgcta atgaagcata aggttgggtg aatcgttgtt cgctaccgtc ttcttctttg
    10921 atgg
//
LOCUS       DAJTVE010000132        10895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000132 DAJTVE010000000
VERSION     DAJTVE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..613
                     /locus_tag="OO439_003360"
                     /pseudo
     CDS             1..613
                     /locus_tag="OO439_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /locus_tag="OO439_003361"
     CDS             610..1224
                     /locus_tag="OO439_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6317648.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OO439_003362"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OO439_003362"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6317649.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="OO439_003363"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="OO439_003363"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6317650.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="OO439_003364"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="OO439_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6317651.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="OO439_003365"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="OO439_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6317652.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6340)
                     /gene="phoE"
                     /locus_tag="OO439_003366"
     CDS             complement(5291..6340)
                     /gene="phoE"
                     /locus_tag="OO439_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6317653.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            6628..7731
                     /gene="proB"
                     /locus_tag="OO439_003367"
     CDS             6628..7731
                     /gene="proB"
                     /locus_tag="OO439_003367"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6317654.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7743..8996
                     /gene="proA"
                     /locus_tag="OO439_003368"
     CDS             7743..8996
                     /gene="proA"
                     /locus_tag="OO439_003368"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6317655.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9111..9186
                     /locus_tag="OO439_003369"
     tRNA            9111..9186
                     /locus_tag="OO439_003369"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9144..9146,aa:Thr,seq:cgt)
     gene            9792..9950
                     /locus_tag="OO439_003370"
     CDS             9792..9950
                     /locus_tag="OO439_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317656.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2574 c         2778 g         2783 t
ORIGIN      
        1 gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga
       61 cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgc gtattaatcg
      121 ccagctgatc tctttacgca taatgcaaca ggttaaggcc accggaagtc cggttctgga
      181 tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac tcggtgatta
      301 ctcctagctt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 tgggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac
      421 gcagctctca cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 tggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga gcgattaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gcggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgttttgt tgaacagacg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag agaacggatc agacagtgaa tttctacgtt attatcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtctgagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt caggacgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ctgcatgacc cgctaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttccccgcc ggagtgaccc
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
     2281 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
     2341 accacgtttt cgtcagccag aaccgccagc gcagacgcca taccaatgcc gttatctgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg atttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggcat tgacccacgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg
     3781 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccatccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgtcac tggctgcatg cggctacgtt gtacaacatt gctgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg
     4021 aagaagccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgttttaaag gctctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaaatttg ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgagttct ggggctggtg gatggagctt gaagcgcagg
     5041 aatcccgttt tacctacagt taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagacact gaagtggttg aacgactgga gcacacccta cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgt atcacatccg
     5281 gcaatatcca ttaaaactga taggtcatgc caaccgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatac
     5461 cttcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 aatcgccacc aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttctg ctgtgcagta tcactctccg ctttatttcc ggcaaactcc gcttcccacc
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcacc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctggcgttat cactcatata atgcataact ttgccataga
     6241 catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat gcagatgcca
     6301 caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt
     6361 aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat
     6421 tgttaaagat atattacaga ttaataatat tcttaaaatg tggtaattta ttaaatctgt
     6481 aataaaagcg taaacaactg ccgctaggct tgctgatccc gcgcaacaaa acgccatgct
     6541 ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt
     6601 tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc
     6661 ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat tgttgaactt
     6721 gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc
     6781 gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac tatcgcctcg
     6841 aaacaactgc tggcagcggt agggcagagt cgactgattc aactgtggga acagctgttt
     6901 tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt
     6961 gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg
     7021 gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aagtcggcga taacgataac
     7081 ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa
     7141 aaaggtttgt ataccgctga cccgcgcagc aatccgcagg cagaactgat taaagatgtt
     7201 tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact
     7261 ggcggcatga gtaccaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc
     7321 attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc
     7381 ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt
     7441 gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tctggaacgc
     7501 ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa
     7561 gtcatccgca tttgcaacct cgaaggccgc gatatcgccc acggcgtcag tcgttacaac
     7621 agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc aatactggga
     7681 tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagctggc
     7741 cgatgctgga acaaatgggc attgctgcga agcaagcctc gtataaatta gcgcaactct
     7801 ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg
     7861 aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat ggccttagcg
     7921 aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgacgatg
     7981 tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc ggcgtactgg
     8041 acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg
     8101 aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg
     8161 tgatcctgcg cggtggcaaa gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc
     8221 aggacgccct gaaatcctgc ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg
     8281 accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc
     8341 gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag
     8401 gtggtatagg cgtatgccat atttacgttg atgaaagtgt agagatcgct gaagcattaa
     8461 aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc
     8521 tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa
     8581 gcggcgtgac attacacgca gatgcagctg cactggcgca gttgcaggca ggccctgcga
     8641 aggtggttgc tgttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg
     8701 tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac
     8761 actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg
     8821 attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc
     8881 tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg
     8941 aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa
     9001 aaccgggtga tgcaaaagta gccatttgat tcataaggcc attgacgcat cgcccggtta
     9061 gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag
     9121 ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg
     9181 gcaccattaa gaaaattaat aacttagctt aacttctcta agaaattgta tcttctgtta
     9241 tcacttacaa acgcctatac gttgttatgg gctttggtag tgtaacacgt atgggcgagt
     9301 agttcccagg ctcaacctcg taacaagttc ctttgaagct ggaagacgtg ccaccgtctc
     9361 taaaccttct accaaaactc ttagcaagat gttccacacc tggcgcggtg tccaatgatg
     9421 ctacacgtcg tgagacgttg cagaggataa acaggctaaa tgatgggaag agttttaagt
     9481 gcaatagtgg attaaagccc acctcttcaa aacctaactt gatcgggaaa gaatcaagct
     9541 ctagcacggt ttcgtccacc ggagtacaac gggtgcctga taagagaaga actgctaaga
     9601 cttcatagct tttgtgaaaa agcccttttt agtggatacg cctacggcgc ttattttttg
     9661 gtataaccat caaaggcgct ttatggctcc tattgtcttg aaaatgtatc tttacttgta
     9721 ttgtgtttta tgcgtagtct ccctgtgtag tatgtaactt gttaattttc atatgaatac
     9781 agaggcacac gatgaaaaac attgcagcaa tcaaacgtaa taaccgcaag attcacgctc
     9841 gtaagttcct gtctacgcca gaaggaaaag cctggctaga gcgtaaacag agagagaacg
     9901 aagaaagaaa actccttagt gagttgaaat ggcttagaga ggatttctga aaatctccag
     9961 aacgcaacag gacgcattct aacgctgttt tcattactcg gtatagggat aggtgatgtg
    10021 tgtttaatgc gtctgtgcgc ttgttttgtg ccttattttg cattttcata atgcggaaga
    10081 gaacacggaa aaccttaaaa ttaattattg gggtatttaa ggctattttc aggtgattta
    10141 agggtattaa acttgattta attgattaaa atataaacag ataattatgt atcttattga
    10201 tttataaggt tatttttaag taaaggtaaa taaaaaataa atagccataa atagctataa
    10261 atacatctta atacctatat cccttccctt atggctagat tgcgagaggt gcttaacgcg
    10321 attttcagcg ccccccgatc tggaaaatcc agaatcccca aatatcagca aggaattttc
    10381 ttgcgtaatc aacatcgttt ttacctatta aatcattaca cagaaagcct ttctgtaatg
    10441 tctctcacta aactttcact aaacacgaga atgatcggtt ttatgtcgga tcagatgcta
    10501 gaaacagcac cacgtctcac aagggctgta agcgatgaaa ccagtgttta tgcgggtgca
    10561 ggtcaaaata taggccaaaa tccatttaac ataataaatg ttatatgtgc taaggaacac
    10621 atataacgcc ttgaattgat gtatcagtgg aaaagtgatg gattttttac gtaggagcac
    10681 gaaagggctt ctattgactt ttgtgtgcta tgaaatataa ttaaagtttt ttgtttttat
    10741 tgtgtgatgt ggtgggttac acaggggaat agattaaatg gtgttctgtt tatacaggtt
    10801 tatttgttag atcaacaatt tggcatttct aaacagatgt ttgaacgttg ccaaatgtct
    10861 tgacaacaca agaaaagggc ttgctatgcc aggca
//
LOCUS       DAJTVE010000133        10706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000133 DAJTVE010000000
VERSION     DAJTVE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..716
                     /locus_tag="OO439_003371"
     CDS             <1..716
                     /locus_tag="OO439_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317657.1"
                     /translation="LSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKII
                     FNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGAL
                     SESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLA
                     SFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDK
                     WKRENMTPFMYVLNTPPVI"
     gene            726..1205
                     /locus_tag="OO439_003372"
     CDS             726..1205
                     /locus_tag="OO439_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317658.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1342..2127)
                     /locus_tag="OO439_003373"
     CDS             complement(1342..2127)
                     /locus_tag="OO439_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317659.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2457..2533)
                     /locus_tag="OO439_003374"
     tRNA            complement(2457..2533)
                     /locus_tag="OO439_003374"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2497..2499),aa:Asp,seq:gtc)
     gene            complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OO439_003375"
     CDS             complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OO439_003375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6317660.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3462..3929
                     /gene="rnhA"
                     /locus_tag="OO439_003376"
     CDS             3462..3929
                     /gene="rnhA"
                     /locus_tag="OO439_003376"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6317661.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3926..4648)
                     /locus_tag="OO439_003377"
     CDS             complement(3926..4648)
                     /locus_tag="OO439_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6317662.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4682..5437
                     /gene="gloB"
                     /locus_tag="OO439_003378"
     CDS             4682..5437
                     /gene="gloB"
                     /locus_tag="OO439_003378"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6317663.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5509..6867
                     /gene="mltD"
                     /locus_tag="OO439_003379"
     CDS             5509..6867
                     /gene="mltD"
                     /locus_tag="OO439_003379"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6317664.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6916..7512)
                     /locus_tag="OO439_003380"
                     /pseudo
     CDS             complement(6916..7512)
                     /locus_tag="OO439_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7543..8343)
                     /locus_tag="OO439_003381"
     CDS             complement(7543..8343)
                     /locus_tag="OO439_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6317665.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8584..9498
                     /locus_tag="OO439_003382"
     CDS             8584..9498
                     /locus_tag="OO439_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317666.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OO439_003383"
     CDS             complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OO439_003383"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6317667.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10461..10537)
                     /locus_tag="OO439_003384"
     tRNA            complement(10461..10537)
                     /locus_tag="OO439_003384"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10501..10503),aa:Asp,seq:gtc)
BASE COUNT         2951 a         2450 c         2429 g         2876 t
ORIGIN      
        1 tactcagccc aataacatca tttgattaca ttcattttat cactacacat ccttcaggta
       61 taaaagatac actcgcctgg ttagttaacg cgagtaaatt aatgactgag tttgatgaca
      121 atgggaaaat tatatttaac attaatgcct taaagtatac aaaagcaagt tactttgaaa
      181 tacttggaga gaaatatata aaaatcacaa catccagccc ctggctttta gaaaagctcg
      241 gtaaatatat tttctcttca cgagcgcctc aagttctgga actcgctatt ggctggcgag
      301 gagctttatc tgaatcaata aaaggagtta agttctgtat ctggttttca gtagcctgga
      361 gaaccataga atttattatg agttcagagc gagatctggt taattttctt ggcgattttt
      421 caatggatgt ggccaaagca gttatagccg gtggtgttgc aaccgctatt ggaagcctgg
      481 cttcttttgc ctgtgttagc tttggctttc cagtaattct tgtcggagga gcaattttac
      541 tgacagggat agtgtgtaca gttgttttaa atgaaatcga tgctcaatgc catttatcag
      601 aaaaattaaa atatgcaatt agagatggac taaaacggca acaggaactt gataaatgga
      661 aaagggaaaa catgactcca tttatgtatg ttcttaacac tccacccgtg atatgaggat
      721 taaccatgcg tttacatgtt aaattaaaag agtttctttc gatgtttttt atggccatat
      781 tatttttccc tgcattcaat gcttctttgt tttttaccgg agttaaacct ctatattcaa
      841 taataaaatg ctccacggaa atattttatg attggagaat gttgattctg tgctttggtt
      901 tcatgtcgtt ttccttttta aatattcatg taattttact tacgataata aaattattct
      961 taatcaaaaa aacaaaggta gtcaatttcg ccacagacat aacaatacag ctaactctta
     1021 tatttttact aatagctatc gtaatagcac ctttaatagc tccttttgtc acaggatatg
     1081 tcaatgctaa ttatcacccc tgcggtaata atacgggaat tttcccagga gcaatctata
     1141 ttaaaaatgg catgaaatgt aataatgaat atatttctcg caaggaagat tcagcagtta
     1201 aataattaaa cattctaaaa caaacagttt ttaactccgg attccatgtc ctttcttgca
     1261 ctgtctttgt caagaaaaat ttcttatgca aagaattttg tattatttcc gccagaaatt
     1321 cctggcggca gatataattt attattgata gcggttaacc aaatctttta ccatcgcttt
     1381 ataggtcttt tgcttaagtt gcatgatttg ctgctctgtc atatcagtcg tactggcttc
     1441 attcttgtcg ggcaatggtg gaagcacttt atattcataa ttcaccggcg accaggcagc
     1501 ccactcacct gtagctacat ccaccagcgt gaatcgtacc agatagcgca gtgacatcgt
     1561 gtcagtcaat ttttgatttt tataatctga ccaaactatt gatttcaatc cagagtcata
     1621 ttttcctgtc tgcaacgtat cctggaaaac aataatcgct ttctgatgcc cttttgcagc
     1681 cacaaaacgc agtgcctgca tccagttcat attctcagca ctggcaacat cctcgttctc
     1741 atttttatct ttgtttttgt tacaagtcag aggcttttgc ctgtccggga taccagagaa
     1801 tgtggaaaca gtataatatt ttcgcatctc ctcctgcata atggtttccg gtgcgcggtt
     1861 gccggattga acaagtatta caggagaatt aaggggaacg cggaactcat ctccatcaag
     1921 cgcagcttga atatcctctt cggatacggc cagagtggtt tcattaccga ataaatcttt
     1981 gtctgtcagt tgctggggag tgtttttatt aatattactt tgtgtgactt gagctttttt
     2041 cttcttttca tcaatagaag attcagagac acatcctgca agcagcgaga ataatacaca
     2101 tatacaacaa acccttctta aattcataat cggacactcc ctcgcctgtc ataatcatgg
     2161 taatgtttag ctaacgattt ttaattatat actccacttg aatcagaaca aacaaaaata
     2221 acagtatata taaattcatt aaatcaatag aaaaatgaaa atcataacat tcaaatatat
     2281 ttaaattgaa tgatttgata gtatgattaa atattaatga tatggtctac catatgtgat
     2341 taacacattt acagcataac aaaaccatag accgaagaaa tagcgggatt taaatgcaaa
     2401 aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag tgaatttggc
     2461 ggaacggacg ggactcgaac ccgcgccccc ctgcgtgaca ggcaggtatt ctaaccgact
     2521 gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcac
     2581 ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagccgc
     2641 tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg cccttcttct
     2701 ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca aaaacaacgc
     2761 gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg
     2821 tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt
     2881 ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg ttatctattt
     2941 cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca
     3001 ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca
     3061 acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg
     3121 cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca
     3181 aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt
     3241 tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa
     3301 tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg
     3361 tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagactta
     3421 tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa
     3481 ttttcaccga tggttcgtgt ctgggcaatc caggacctgg gggttacggc gctattttac
     3541 gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta
     3601 tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt
     3661 tgagtaccga cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa
     3721 aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc
     3781 ttgatgctgc cctagggcaa catcaaatca aatgggaatg ggttaaaggc catgccggac
     3841 acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc ggcaatgaat cccacactgg
     3901 aagatacggg ctaccaagtc gaagtttaag cttgtggttt acgacattgc cgggtggctc
     3961 caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcgggttt agcgttaaag
     4021 gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat
     4081 tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgcagca
     4141 cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac atccggctgt
     4201 tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt aaactaatgg
     4261 gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg gtttcacgca
     4321 ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg
     4381 atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc attccttgcg
     4441 cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat
     4501 tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct
     4561 ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca ggagccacga
     4621 cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacacta agaggtaatc
     4681 tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga
     4741 agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc
     4801 cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg atcacgttgg
     4861 cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac
     4921 acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca
     4981 tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt acttcagtaa
     5041 accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtaggt tgtttgaagg
     5101 gacagcatca caaatgtatc aatcacttaa taagttaagt gcgttacctg acgatacatt
     5161 ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga gtattcttcc
     5221 gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca
     5281 aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga
     5341 agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg
     5401 ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc
     5461 cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa
     5521 gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag
     5581 cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca
     5641 cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc
     5701 gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat
     5761 ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg
     5821 atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg
     5881 gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc
     5941 attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc
     6001 gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa caaaatgttt
     6061 gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag
     6121 gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg
     6181 caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc
     6241 aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac
     6301 ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag
     6361 ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg gccgcagtac
     6421 gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctcgcttc aggcgaaatt
     6481 gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc
     6541 gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag caccaaagat
     6601 ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag tttgacgatt
     6661 ggtgcaggca gtagcgcaca gcggttggca aacaacagcg atagcattac gtatcgtgtg
     6721 cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg
     6781 atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg
     6841 aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca ccgattcccc
     6901 cggtgccttt tttatttatg ccgctttatg tgcttctacc atgatgatat cactggtgaa
     6961 agagccatca gtctgcaagg aaaaatacgt tctcacctct gtcgatgcgc tctgttggta
     7021 aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcacgaccc atgaagaaaa
     7081 ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt cattgattaa
     7141 cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac
     7201 cgcctgtaac cagatgtcgc gcactgggtg acccggagac attacatcca tcacaatcag
     7261 cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca
     7321 gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact
     7381 tgcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cggcttgtgc
     7441 cacgacatcc agcatgtggg cagataagtc atacgccacc accgcgctca cgttttgcgc
     7501 agcgccaaac gttggtctgc cctgcggcag acctgacata ccttatttat caggcttgcc
     7561 gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca gtacagaagc
     7621 ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg
     7681 gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa
     7741 cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct
     7801 gtggtgagct atctgatcac caataggaag taactgctta ctatagacat ccacgcccag
     7861 actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata
     7921 gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca
     7981 gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac
     8041 gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta ccaactctgg
     8101 cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat tctttaatac
     8161 cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc
     8221 ttcggtactt aacggttctc cgggtggtaa tgcctggccg atgctcgcaa aagaccccgg
     8281 cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg
     8341 cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg
     8401 ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg
     8461 cttacaccgg tcagaaattt gctcattatt gagaacatta aatcggatga catatcacgc
     8521 atcctgatgg tttaatgaca aataaattca aaatatctca tctattttgc aacaggagca
     8581 aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc
     8641 tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa
     8701 aagctggaaa tgaaacttgg cgttagcctg cttaatcgga ccacgcggca acttagcctg
     8761 acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg
     8821 gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg gatcgatgcc
     8881 gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg tgaacgctat
     8941 ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa
     9001 gtggatgtcg cgatacgcgc tggtacgtta acggactcca gcttacgtgc caggccgtta
     9061 tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca
     9121 gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc
     9181 aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta cggtttgtca
     9241 tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg
     9301 tccgactaca tgatcgacaa agaaatcgct cgcggagaat tggtggagtt aatggcagat
     9361 aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt
     9421 acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga
     9481 gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg
     9541 ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc
     9601 ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc
     9661 cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcaatacga
     9721 gcaataacct catctttcag tgccttacca taagccagcg tcatatagga agtgatatga
     9781 ataccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca
     9841 atctggttgg tagcgatgtt ttcagcgcca acagcagcaa tcgctttttc catcaacggg
     9901 atcgtgaagt tggaaatacc gatctcacgt gtcagacctt gttttttggc ctccagcagc
     9961 gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc
    10021 agatcaacat aatcggtacg caatttttgc aggctctctt ttagactcgg gatcaatttg
    10081 tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg
    10141 ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg tgcagtatca
    10201 attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttg
    10261 agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat
    10321 tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc ataaaatgca
    10381 gaggattttt gcgattctgg caataataga tatacagacg aaaaaaaacc tcgagcgtta
    10441 actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    10501 gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg
    10561 gttttatgac cggcttactg cttaatttga tgcctggcag tttatgaatc acttcgggct
    10621 gttgcttcgc aacgttcaaa agctttcgct tttgtcctac tctcttatcg agtagtgaac
    10681 tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAJTVE010000134        10591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000134 DAJTVE010000000
VERSION     DAJTVE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO439_003385"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO439_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6317668.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OO439_003386"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OO439_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6317669.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OO439_003387"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OO439_003387"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6317670.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OO439_003388"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OO439_003388"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6317671.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OO439_003389"
     CDS             complement(5254..6294)
                     /locus_tag="OO439_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6317672.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OO439_003390"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OO439_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6317673.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OO439_003391"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OO439_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6317674.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OO439_003392"
     CDS             7229..7669
                     /locus_tag="OO439_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6317675.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OO439_003393"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OO439_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6317676.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OO439_003394"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OO439_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6317677.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO439_003395"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO439_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6317678.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2429 a         2736 c         2703 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAJTVE010000135        10590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000135 DAJTVE010000000
VERSION     DAJTVE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..824
                     /gene="narU"
                     /locus_tag="OO439_003396"
     CDS             <1..824
                     /gene="narU"
                     /locus_tag="OO439_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6317679.1"
                     /translation="PVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMN
                     DIASSRASIDDQLPVLQRLHLRLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRL
                     AFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFY
                     AVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFI
                     SAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            906..4646
                     /gene="narZ"
                     /locus_tag="OO439_003397"
     CDS             906..4646
                     /gene="narZ"
                     /locus_tag="OO439_003397"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6317680.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4643..6187
                     /gene="narH"
                     /locus_tag="OO439_003398"
     CDS             4643..6187
                     /gene="narH"
                     /locus_tag="OO439_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6317681.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6187..6882
                     /gene="narW"
                     /locus_tag="OO439_003399"
     CDS             6187..6882
                     /gene="narW"
                     /locus_tag="OO439_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6317682.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            6879..7559
                     /gene="narI"
                     /locus_tag="OO439_003400"
     CDS             6879..7559
                     /gene="narI"
                     /locus_tag="OO439_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6317683.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            7638..8531
                     /locus_tag="OO439_003401"
     CDS             7638..8531
                     /locus_tag="OO439_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6317684.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(8627..9472)
                     /gene="nhoA"
                     /locus_tag="OO439_003402"
     CDS             complement(8627..9472)
                     /gene="nhoA"
                     /locus_tag="OO439_003402"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6317685.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            9645..10214
                     /locus_tag="OO439_003403"
     CDS             9645..10214
                     /locus_tag="OO439_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6317686.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10218..10445)
                     /gene="pptA"
                     /locus_tag="OO439_003404"
     CDS             complement(10218..10445)
                     /gene="pptA"
                     /locus_tag="OO439_003404"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6317687.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
BASE COUNT         2469 a         2781 c         2868 g         2472 t
ORIGIN      
        1 tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt
       61 cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcct
      121 ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgacgaccag ctccctgtct
      181 tacaacgcct gcatctccgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta
      241 tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc
      301 tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta
      361 tttccgataa attcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt
      421 tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt
      481 acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga
      541 tgatcgccgt catctttcgc cagataacca tttatcgggt gaagatgaaa ggcggtagtg
      601 atgagcaagc tcagaaagaa gccatcaccg aaacggcggc ggctctgggc tttatctcag
      661 ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga
      721 ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc
      781 tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat
      841 gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc
      901 atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg
      961 ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc
     1021 gttggcagtt cgacaaaatt gtgcgttcca cccacggtgt taactgtaca ggctcctgta
     1081 gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc
     1141 cgcgcactcg ccctgaccag cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt
     1201 actcctggta tctctacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga
     1261 ttgaactgtg gcgtgaagct ctcaagcaac acagcgatcc ggtactggcg tgggcatcga
     1321 ttatgaacga tccgcaaaag tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta
     1381 tccgctccaa ctggcaggaa cttaaccaac tgattgccgc cgctaacgtc tggaccatca
     1441 aaatctatgg tccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggtgt
     1501 catacgctgc cggaacgcgt tatctgtcgc tgttaggcgg cacctgttta agcttctacg
     1561 actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc
     1621 cggaatctgc cgactggtat aactccagtt acatcatcgc ctgggggtct aacgtaccgc
     1681 agacacgtac gccggacgcc cacttcttta ccgaagtacg ttacaaaggc accaaaacta
     1741 tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga
     1801 aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc
     1861 atctcgataa tcccagcgac tactttatca actactgccg ccgctatagc gacatgccga
     1921 tgctggtaat gctggaacct cgcgacgatg gtagctacgt acccgggcgc atgatccgcg
     1981 cgtctgacct ggtagatgga ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg
     2041 ttaataccgc aggtgaattg gtagtgccga atggttcgat tggtttccgc tggggagaaa
     2101 aaggcaaatg gaatctggaa tccattgccg ccggtaagga aaccgaattg tcgttaaccc
     2161 tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa
     2221 atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa
     2281 acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtgctgg
     2341 cgaattacgg cctcgatcgc gggctggaag atgaaaacag cgcgaaagat tacgctgaaa
     2401 tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtaccgcgc cagtatattg
     2461 aaaccatcgc ccgcgaattt gccgataccg cccataaaac gcatggacgc tcaatgatta
     2521 tcctcggcgc tggcgtcaac cactggtatc acatggatat gaactaccgt gggatgatca
     2581 atatgctgat cttctgcggt tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg
     2641 gtcaggaaaa actgcgccca caaaccggct ggttgccgct ggcctttgcg ctcgactgga
     2701 accgcccacc gcgccagatg aacagtacct cgtttttcta caatcattcc agccaatggc
     2761 gctatgaaaa agtctctgct caggagttac tttcaccgct tgccgatgcc agtaagtaca
     2821 gcggtcatct gattgatttc aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc
     2881 cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc
     2941 ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac
     3001 cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct
     3061 cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc
     3121 agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg
     3181 cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct
     3241 gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca
     3301 cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat
     3361 cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg
     3421 gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag
     3481 agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag
     3541 gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct
     3601 ttacctcgct cgggccattg atggacaaac ttggcaatgg tggtaaaggg atttcgtgga
     3661 atacccagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg
     3721 cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg
     3781 caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg
     3841 ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata
     3901 ttcaggcgca gccgcgtaaa attatctcca gccccacatg gtccggtctg gaaagcgatc
     3961 acgtctccta taatgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt
     4021 cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg
     4081 tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag atcccgccaa
     4141 acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact
     4201 caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga
     4261 tcagcgaaac cgatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtcttta
     4321 acgccaacgg cgcgctgacg gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga
     4381 ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca
     4441 tgcgcggcgg cattcataac tcggtcaccc gtgtttgccc gaaaccgacg catatgattg
     4501 gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcggc tcgaaccgcg
     4561 acgaattcat catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg
     4621 atcaggtaca ggaggcgaaa aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct
     4681 cgacaaatgt atcggttgcc atacctgttc ggtgacctgt aaaaacgtct ggaccggacg
     4741 cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc
     4801 gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa
     4861 gatacgcccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt
     4921 ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag
     4981 cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcgctgattg acggtaagcg
     5041 gatggacaaa gtgatctggg ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa
     5101 acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa
     5161 caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc
     5221 gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca
     5281 ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt
     5341 caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg
     5401 ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct
     5461 ttacgacgcc gaccgcattg aggaagcggc gagcaccgag cgcgaagttg acctctatga
     5521 acgtcagtgc gaagtgttcc tcgatccaca cgatccctca gtgatcgagg aagccctgaa
     5581 acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc
     5641 gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc caatggtctg
     5701 gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag
     5761 cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat
     5821 gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg
     5881 ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt
     5941 aggcctgagc gtcgcccagg tcgaagaaat gtatcgctat ctcgccattg ccaactatga
     6001 agatcgtttt gttatcccga ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg
     6061 caacggctgc ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct
     6121 gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg
     6181 cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga tgagctgttg
     6241 tgggaatgca aggaggacgc gctggcgttg atccgccgcg acgcgccgat gcttacggat
     6301 ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg
     6361 tttgaccgcg ggcgcaccac gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc
     6421 gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg
     6481 gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct
     6541 gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt
     6601 cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg
     6661 ggaagtatac tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac
     6721 acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac
     6781 gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc
     6841 gcgccgcagt atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct
     6901 ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc
     6961 gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc gataaacgcg
     7021 ggatggtgat atggtcgaat ctgttccata tcggcatttt ggggattttc ttcgggcact
     7081 tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac
     7141 aactcatggc gatggtgctc ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag
     7201 gactgctgtg gcgcagtctg acaaatcagc gcgtgcgtgc cacttccact acgccggata
     7261 tcatcatcat gagcattttg ctgatccagt gtctgttggg gctaagtacc ataccgtttt
     7321 cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg
     7381 tcactttccg tggtggatcg tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc
     7441 atctggtgtt gggaatgacc atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt
     7501 ggagcgcgcc gtttgagtac tttactcgtc gatatcaaat tgtgcgttcg cggcgataat
     7561 ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca
     7621 gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctctcaac
     7681 catttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcacaaa
     7741 tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg
     7801 acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg
     7861 cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct
     7921 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
     7981 gtatttcgct ggaacaaggt acgccgggct ttgaactgcc gctggagggt gaagtacgcg
     8041 cggcgattat caacgcattg catcttactg aagacgatat tctgccgggt ttgccgatcc
     8101 aggtcgcgac gaccgggcat tctaaagtga tgatcccatt gaagccggaa gtggatatcg
     8161 acgctctttc gcctgacctt aatgcgctga ccgctatcag taaacagatt ggctgcaatg
     8221 gtttcttccc gttccagatc cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc
     8281 ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
     8341 ggttggtaca tcacaacgta ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc
     8401 aggggcgcgc attggggcgt gacggcatga ttgaagtgac ggtgacaatt cgtgataacc
     8461 aaccggaaaa agtcaccatt tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa
     8521 ttgaaatctg aaatcgttgc ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg
     8581 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
     8641 ccggatgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta aagccatgtt
     8701 tcgcatcatc cacgcccaga ccaaactgtt cctgcatcac ggcatataac gacgccacat
     8761 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
     8821 tcagtgtcag cttgccgccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
     8881 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
     8941 cgcagagatc gaagcggtac atcggctgcc aatgctgatg atgattaaac tgcaacaccc
     9001 aatcatcacc ctcctgcaac aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg
     9061 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
     9121 tttccccttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
     9181 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc
     9241 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
     9301 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtca aggttttcaa
     9361 acggaatggt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
     9421 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
     9481 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
     9541 gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc
     9601 gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc
     9661 ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc ttgatcacca
     9721 gtttcgatga gcaatcccag cggcgtaaca ttatggctgc agcctggtca atgccggtgg
     9781 agtttgaacc tccacgcgtg gcaattgtgg tagataaatc gacatggacc agggagttga
     9841 ttgaacgtaa cggtaagttt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt
     9901 gggcggtggg aagtgtgtcg gtgcgtgaag aagataaatt taattgctat ggcattccgg
     9961 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg
    10021 agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggcgagg
    10081 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg
    10141 ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttactagc ggcaagcgtg
    10201 ttacggcagg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg
    10261 ggctcgattt cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca
    10321 atgcttatcg agctatcttt actgttcaga tggcgaataa taacgtcggt aatatctgca
    10381 gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc
    10441 ggcatgatta cgctcctttt gaaagatgtc agtaaaatta acacacattg tgaatgtcat
    10501 gtgtccggta aagcgtttat acgacaataa tgcccagggc aagattacga aagcccgctc
    10561 cccgtaagga ctgacgccag gtagtttctg
//
LOCUS       DAJTVE010000136        10538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000136 DAJTVE010000000
VERSION     DAJTVE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(45..1328)
                     /gene="aroA"
                     /locus_tag="OO439_003405"
     CDS             complement(45..1328)
                     /gene="aroA"
                     /locus_tag="OO439_003405"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6317688.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1399..2487)
                     /gene="serC"
                     /locus_tag="OO439_003406"
     CDS             complement(1399..2487)
                     /gene="serC"
                     /locus_tag="OO439_003406"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6317689.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2686..3378)
                     /locus_tag="OO439_003407"
     CDS             complement(2686..3378)
                     /locus_tag="OO439_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6317690.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3508..5268
                     /locus_tag="OO439_003408"
     CDS             3508..5268
                     /locus_tag="OO439_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6317691.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5674..6531
                     /gene="focA"
                     /locus_tag="OO439_003409"
     CDS             5674..6531
                     /gene="focA"
                     /locus_tag="OO439_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6317692.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6586..8868
                     /gene="pflB"
                     /locus_tag="OO439_003410"
     CDS             6586..8868
                     /gene="pflB"
                     /locus_tag="OO439_003410"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6317693.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9060..9800
                     /gene="pflA"
                     /locus_tag="OO439_003411"
     CDS             9060..9800
                     /gene="pflA"
                     /locus_tag="OO439_003411"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6317694.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9893..10069)
                     /locus_tag="OO439_003412"
     CDS             complement(9893..10069)
                     /locus_tag="OO439_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6317695.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10169..>10538
                     /locus_tag="OO439_003413"
     CDS             10169..>10538
                     /locus_tag="OO439_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317696.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIK"
BASE COUNT         2713 a         2636 c         2496 g         2693 t
ORIGIN      
        1 gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc tgcctggcta
       61 atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt gggatcaaga
      121 atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat ccggtgatca
      181 ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg aatgtaatcg
      241 tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc aaacaggcga
      301 tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc ttttgcaaat
      361 aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc catatcaata
      421 gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat ggtcgcgccc
      481 attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg tccaataccg
      541 gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga agccgaagat
      601 gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc ttttacgaca
      661 aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag attgagtgtg
      721 atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt atcttccggc
      781 gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga gccatcaacg
      841 tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt ttcttgttcc
      901 aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc aatcgggcgt
      961 tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca aagagctgcc
     1021 gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc accttctgcg
     1081 tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga aagcgtatag
     1141 cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct atccagcaga
     1201 ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg gttagaaacg
     1261 ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg taacgtcagg
     1321 gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg gctgtgggga
     1381 ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga agtctgtcag
     1441 cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc cgccgaccac
     1501 acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga acaatttgtc
     1561 aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag ctttcgccac
     1621 gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg ctttttgctg
     1681 attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa agaccagacc
     1741 agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat cgttgaggat
     1801 ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat cttcacgaac
     1861 gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt aaatcacgcc
     1921 ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg cggcgaccac
     1981 cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg tttcattcgg
     2041 gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg gcttaaccgc
     2101 gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt attttttcgc
     2161 ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg tggttttatc
     2221 accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat ggcagaataa
     2281 taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg cttcctctgc
     2341 aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac caagaccgtt
     2401 ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca ttgccggacc
     2461 agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc tattaacccg
     2521 cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg ttttatcatt
     2581 cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct tgcatcacac
     2641 aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg tcacccgttt
     2701 tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt gtttttcccc
     2761 cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg actccggcac
     2821 cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt tcaccttgtc
     2881 atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca gacgtacctg
     2941 ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt ccgtcatgtt
     3001 ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa ccggcttacc
     3061 ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca ggcggtataa
     3121 caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat cataaaacgc
     3181 cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca gcgacatctg
     3241 tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa aggtataaag
     3301 actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg ccatacggtg
     3361 gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa agcatagtca
     3421 acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt attattgcgc
     3481 gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg caaagatgcc
     3541 gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg ctttcagatt
     3601 gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat tcgcgacaaa
     3661 gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc gctggcttct
     3721 gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga tttctggctg
     3781 ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg gttcccactg
     3841 accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc gttttatgat
     3901 ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa cgaagatcgt
     3961 ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta tattccgatg
     4021 aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac ccgcaacgaa
     4081 gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg cattattgct
     4141 gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc agcagtagtt
     4201 gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga cggttcgctt
     4261 ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg tacctgcttt
     4321 gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt gaccgagctg
     4381 ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt cgatgatgaa
     4441 gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg tttaatctcc
     4501 tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt ctccggcacc
     4561 acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa agaagtttat
     4621 attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc tggcatgtcc
     4681 gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag ccatttacgt
     4741 gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct gaacctcatc
     4801 gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct gttgggtctg
     4861 gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa agccatgctg
     4921 gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac gatggagttt
     4981 aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca aacgttgtta
     5041 ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt tgttcgcatg
     5101 tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc ggcgttttac
     5161 ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc gttgttgaag
     5221 gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa tcaaatagcc
     5281 tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt tttaatgccc
     5341 cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa tatcggtaat
     5401 ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc ccttttaatt
     5461 aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt taaaatttat
     5521 ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc gagttacggg
     5581 cctataagcc aggcgagata tgatctatat caatttctca tctataatgc tttgttagta
     5641 tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc ttttgatctt
     5701 ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa agcaacgaaa
     5761 catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc aatcgcattc
     5821 gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat ggcaaaactg
     5881 gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg agccgatctc
     5941 tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac ctggggtcag
     6001 ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact gctgtttgta
     6061 cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg actaaacgtc
     6121 ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg tcttggtatc
     6181 ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg cagcctgatg
     6241 gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg ttttgagcac
     6301 agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt cgcatccccg
     6361 gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac cgtgatgaat
     6421 ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg tggtttgttg
     6481 gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta atggttgtcg
     6541 aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc cgagcttaat
     6601 gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa tgaagtaaac
     6661 gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc cttcctggct
     6721 ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt taaactggaa
     6781 aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat cacctctcac
     6841 gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac tgaagctccg
     6901 ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc ctgcaaagcg
     6961 tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg taaaactcac
     7021 aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa atctggtgtt
     7081 ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta ccgtcgcgtt
     7141 gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac ttctctgcag
     7201 gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga agaaatcgct
     7261 gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg ctacgacatc
     7321 tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg ctacctggct
     7381 gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac cttcctggat
     7441 gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc gcaggaaatg
     7501 gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc ggaatacgat
     7561 gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat gggcctcgac
     7621 ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta caccatgggt
     7681 ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct gaacttcaag
     7741 aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa cgatgacctg
     7801 atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag cccgatgatc
     7861 gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac catgctgtac
     7921 gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa gtctgaaccg
     7981 atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca cttcatggac
     8041 tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca cgacaagtac
     8101 agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac catggcgtgt
     8161 ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata tgcgaaagtt
     8221 aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg cgaatacccg
     8281 cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt agaacgtttc
     8341 atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca gtctgttctg
     8401 accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga cggtcgtcgt
     8461 gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca gaaaggtgca
     8521 gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga tggtatctcc
     8581 tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg taagaccaac
     8641 ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg tggtcagcac
     8701 ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa cccggaaaaa
     8761 tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc gctgactaaa
     8821 gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt agatttgact
     8881 gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc ttttttagca
     8941 cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca gactcgacgt
     9001 agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga aacaccgcaa
     9061 tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac ggcccgggta
     9121 ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt cataaccgcg
     9181 acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg aaagaggtgg
     9241 tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc ggcggtgagg
     9301 caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa gaaggcattc
     9361 atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt gatgaactgc
     9421 tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag atccaccaaa
     9481 atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg gcgaacaaaa
     9541 atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat gacgattcag
     9601 cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc gagcttctcc
     9661 cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac aaactcgacg
     9721 gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt gagcagtacg
     9781 gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt tgccgctttt
     9841 ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc atctaccata
     9901 tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg agttccgtca
     9961 cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt gcggtcaatg
    10021 aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata attttacctc
    10081 cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac gtgatgattc
    10141 aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact ttgccacttt
    10201 ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag gcttatcctc
    10261 gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc gcttattcaa
    10321 tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg aacgattgcg
    10381 cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc gtctgacacc
    10441 aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt taatgccttt
    10501 actggttggt ttcacacgtg aatatcctga tattaaag
//
LOCUS       DAJTVE010000137        10420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000137 DAJTVE010000000
VERSION     DAJTVE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            132..2000
                     /gene="kup"
                     /locus_tag="OO439_003414"
     CDS             132..2000
                     /gene="kup"
                     /locus_tag="OO439_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6317697.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2167..2586
                     /gene="rbsD"
                     /locus_tag="OO439_003415"
     CDS             2167..2586
                     /gene="rbsD"
                     /locus_tag="OO439_003415"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6317698.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2594..4099
                     /gene="rbsA"
                     /locus_tag="OO439_003416"
     CDS             2594..4099
                     /gene="rbsA"
                     /locus_tag="OO439_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6317699.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4104..5069
                     /gene="rbsC"
                     /locus_tag="OO439_003417"
     CDS             4104..5069
                     /gene="rbsC"
                     /locus_tag="OO439_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6317700.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5094..5980
                     /gene="rbsB"
                     /locus_tag="OO439_003418"
                     /pseudo
     CDS             5094..5980
                     /gene="rbsB"
                     /locus_tag="OO439_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6106..7035
                     /gene="rbsK"
                     /locus_tag="OO439_003419"
     CDS             6106..7035
                     /gene="rbsK"
                     /locus_tag="OO439_003419"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6317701.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7039..8031
                     /gene="rbsR"
                     /locus_tag="OO439_003420"
     CDS             7039..8031
                     /gene="rbsR"
                     /locus_tag="OO439_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6317702.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7997..9424)
                     /gene="mdtD"
                     /locus_tag="OO439_003421"
     CDS             complement(7997..9424)
                     /gene="mdtD"
                     /locus_tag="OO439_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6317703.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9447..10139)
                     /locus_tag="OO439_003422"
     CDS             complement(9447..10139)
                     /locus_tag="OO439_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6317704.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2547 a         2516 c         2748 g         2609 t
ORIGIN      
        1 atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg ccgttaatcg
       61 tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat atttcaacga
      121 aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga
      181 ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt
      241 ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta tcgctgatct
      301 tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg cgggcagata
      361 acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc
      421 gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag
      481 tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa atcgtcgccc
      541 cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta ttatttatga
      601 ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt
      661 ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa gtgctgcatg
      721 cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt tcttttattg
      781 cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct gatatggggc
      841 actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc
      901 ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt aagaacccgt
      961 tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc gcactggcga
     1021 cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt caggcggtac
     1081 gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa
     1141 tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct
     1201 ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtgc
     1261 tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg
     1321 ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg
     1381 ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg tttatcgtga
     1441 tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa catggtaact
     1501 ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg
     1561 cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac
     1621 ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa gacgcgccat
     1681 atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg
     1741 tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac cgctgcggtc
     1801 tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga
     1861 tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac
     1921 gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt atcgaactgg
     1981 gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg cgtttttgtt
     2041 ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga
     2101 tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc gctgatggag
     2161 aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg
     2221 ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg
     2281 cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc
     2341 acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca acataatccg
     2401 caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc
     2461 attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg
     2521 gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg
     2581 ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg
     2641 cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga tagcgctggt
     2701 gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta tctatactcg
     2761 cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc cgaaatcttc
     2821 ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc agttgaccat
     2881 tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa
     2941 aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct ttaaaagcga
     3001 caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
     3061 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
     3121 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg gtattgtcta
     3181 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
     3241 cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
     3301 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc cgggagatat
     3361 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
     3421 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
     3481 agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
     3541 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
     3601 taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
     3661 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggcagtgag
     3721 tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa ttggtctgct
     3781 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac gccccaaagt
     3841 gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
     3901 actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat cggagatgcc
     3961 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     4021 atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg gcaagcttaa
     4081 tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc cgttatttca
     4141 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     4201 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     4261 cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatcg
     4321 acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     4381 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc gcaattggtg
     4441 cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct acgctggtta
     4501 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
     4561 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     4621 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     4681 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     4741 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     4801 tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg gggactggct
     4861 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     4921 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     4981 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     5041 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     5101 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     5161 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     5221 cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg gtggtgctgg
     5281 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     5341 ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg
     5401 ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa agtgaagtgg
     5461 tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat tacatcgcga
     5521 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt acatccgcag
     5581 cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt aatgttcttg
     5641 ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc tgttgaccgc
     5701 tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg gcgcgctgcg
     5761 cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg gtacaccgga
     5821 tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc tacccgatca
     5881 gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga aagttcaggc
     5941 taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag gttgtatgac
     6001 ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga aaagcagggc
     6061 acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca aaacgcaggc
     6121 agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca atcttttcct
     6181 actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg caaaggcgcg
     6241 aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc ctgtacgggt
     6301 gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat tgatattact
     6361 ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt taatggtgaa
     6421 ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc ggcgctggtg
     6481 gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct ggaatcacca
     6541 ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat cgttgcgctt
     6601 aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga cattattacg
     6661 ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga tgaagatgca
     6721 gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat tactttagga
     6781 agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg attccgggtg
     6841 caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat cacggcgttg
     6901 ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc tgcgattgcc
     6961 gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga cgcattttta
     7021 gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc gggcgtttct
     7081 acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc gattaccgcc
     7141 aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc gcgtagcctc
     7201 aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa tcctttctat
     7261 tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag tctcgtcctt
     7321 tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct gatgcaaaaa
     7381 cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg tgaaatcatg
     7441 caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga tggcgacagc
     7501 gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata tctgatcgat
     7561 aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc ggcgcgcctg
     7621 cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc tgatggctat
     7681 gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca actgttatca
     7741 catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt tggcgtttac
     7801 caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat tggctatgac
     7861 gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca accgaaagat
     7921 gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc gacccttcag
     7981 caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta gatttacgct
     8041 gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt gctgctgaag
     8101 caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca gtcgttgtgc
     8161 cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct aaactaatcg
     8221 acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca tcggtcagat
     8281 cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc cctaatataa
     8341 acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag aactgagcga
     8401 tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc agacgacgta
     8461 agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt gccatcatac
     8521 agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc ataaggaacg
     8581 atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt gagaaagtgc
     8641 gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca tggagaatat
     8701 agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag ctggcgacaa
     8761 tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg ccaaacagca
     8821 aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata tgtttgcgcg
     8881 cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag tgccaggttg
     8941 cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc agacccggca
     9001 tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc agtaaggcca
     9061 gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg aagacaacca
     9121 gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac agactcacgg
     9181 caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt accggaataa
     9241 gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga ggagaacgat
     9301 taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca agtgcctgca
     9361 tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc tttttatcgc
     9421 tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag cgcctgacaa
     9481 gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc ctggtgcaga
     9541 tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata aaccgaatgg
     9601 aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat atgctcatgc
     9661 caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa tgccgccatt
     9721 tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt tgctgccagt
     9781 aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat gacctgatga
     9841 aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt ccagttcgat
     9901 tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt tgccgttaac
     9961 gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc cagctcaatc
    10021 tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg cgccagcttc
    10081 tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct taaaggcatt
    10141 acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt gtgattacgg
    10201 ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg ctgaaaaaat
    10261 gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg
    10321 cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg ttctcctgag
    10381 aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAJTVE010000138        10352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000138 DAJTVE010000000
VERSION     DAJTVE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO439_003423"
     CDS             <1..154
                     /locus_tag="OO439_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6317705.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO439_003424"
     CDS             258..1172
                     /locus_tag="OO439_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6317706.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO439_003425"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO439_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6317707.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSITVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO439_003426"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO439_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6317708.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO439_003427"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO439_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6317709.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO439_003428"
     CDS             5467..7149
                     /locus_tag="OO439_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6317710.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO439_003429"
     CDS             7201..8358
                     /locus_tag="OO439_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6317711.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO439_003430"
     CDS             complement(8324..8446)
                     /locus_tag="OO439_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317712.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO439_003431"
     CDS             8655..10340
                     /locus_tag="OO439_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6317713.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2202 c         2212 g         2910 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattac ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa ag
//
LOCUS       DAJTVE010000139        10314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000139 DAJTVE010000000
VERSION     DAJTVE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..267)
                     /locus_tag="OO439_003432"
     CDS             complement(<1..267)
                     /locus_tag="OO439_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317714.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSG"
     gene            complement(434..1174)
                     /locus_tag="OO439_003433"
     CDS             complement(434..1174)
                     /locus_tag="OO439_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6317715.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1267..1902)
                     /gene="argO"
                     /locus_tag="OO439_003434"
     CDS             complement(1267..1902)
                     /gene="argO"
                     /locus_tag="OO439_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6317716.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2041..2901)
                     /gene="mscS"
                     /locus_tag="OO439_003435"
     CDS             complement(2041..2901)
                     /gene="mscS"
                     /locus_tag="OO439_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6317717.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3259..4338)
                     /gene="fbaA"
                     /locus_tag="OO439_003436"
     CDS             complement(3259..4338)
                     /gene="fbaA"
                     /locus_tag="OO439_003436"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6317718.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4553..5716)
                     /gene="pgk"
                     /locus_tag="OO439_003437"
     CDS             complement(4553..5716)
                     /gene="pgk"
                     /locus_tag="OO439_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6317719.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5766..6785)
                     /gene="epd"
                     /locus_tag="OO439_003438"
     CDS             complement(5766..6785)
                     /gene="epd"
                     /locus_tag="OO439_003438"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6317720.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7157..7588
                     /locus_tag="OO439_003439"
     CDS             7157..7588
                     /locus_tag="OO439_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6317721.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7611..8189
                     /locus_tag="OO439_003440"
     CDS             7611..8189
                     /locus_tag="OO439_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6317722.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8190..8897
                     /locus_tag="OO439_003441"
     CDS             8190..8897
                     /locus_tag="OO439_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6317723.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8885..9562
                     /locus_tag="OO439_003442"
     CDS             8885..9562
                     /locus_tag="OO439_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6317724.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9556..10233
                     /locus_tag="OO439_003443"
     CDS             9556..10233
                     /locus_tag="OO439_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6317725.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10205..>10314)
                     /locus_tag="OO439_003444"
     CDS             complement(10205..>10314)
                     /locus_tag="OO439_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6317726.1"
                     /translation="YDRTDGPNVRRVLEESLPANLTLMMTATGEYRLVD"
BASE COUNT         2475 a         2713 c         2572 g         2554 t
ORIGIN      
        1 ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg ggaacagttt
       61 ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc gggtaaatag
      121 cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg aagccgtcat
      181 atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa tgaaattgcg
      241 catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc gcttaaccgt
      301 ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg tcatggtttt
      361 acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg acgaatacgt
      421 cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc acaggttcta
      481 actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg taagtttcct
      541 gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg ggctgatagt
      601 tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag ccgttcgcca
      661 gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg tctttatagg
      721 catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc gccttcagcg
      781 cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc accgttctca
      841 cagcgcggta gcctttcagg atacttttac catcctgata atcataatct ggctgggtgc
      901 gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg aaggaaatgt
      961 attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc gcggccacgt
     1021 taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc gtaccggagg
     1081 tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg ctaataccca
     1141 ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt taattgactt
     1201 gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt ttccatcaga
     1261 tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca gctgcaaggc
     1321 tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt ttgccgtgcg
     1381 caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca ggaaagaggc
     1441 gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc cgccaaggct
     1501 gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca ctgccagcat
     1561 ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta actcaatatt
     1621 actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg ctacgccgcc
     1681 ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc caccaaaaat
     1741 cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca taatgtggta
     1801 ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg gcagaatcat
     1861 agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata ttccgaatta
     1921 agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga taatattaat
     1981 tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc tcagcgttga
     2041 ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg ggtacgggaa
     2101 gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc agtacacgtt
     2161 ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg atgcaccaag
     2221 ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag actggataat
     2281 attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc caataataaa
     2341 ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa taattttacc
     2401 gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga aaatctgcac
     2461 actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac ggaacgggcg
     2521 gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca aagccagacc
     2581 aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta cacccacgcg
     2641 tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg cagaaagaaa
     2701 atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg cgttggaaat
     2761 catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga tgtttactgc
     2821 ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt ttatgctatc
     2881 gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat ggagaaatac
     2941 actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat aattttgcca
     3001 aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat ccccgcagag
     3061 cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt gaacgcctta
     3121 tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg cctgataagc
     3181 gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg ggccttgaga
     3241 taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga atgctttttc
     3301 cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat cgtagtattt
     3361 cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat aagcttcgtt
     3421 cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg tatcgatgtt
     3481 catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag aaccggaacc
     3541 accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg aaacatattc
     3601 ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt aaacaccgtg
     3661 tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca gttcggtgta
     3721 tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt ggctgttgtc
     3781 cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca tgccgatttt
     3841 ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag attcttcaga
     3901 caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt gtttttcacc
     3961 cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt cagtgtgcag
     4021 gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac cagagatcgc
     4081 gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag cgataaagga
     4141 agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag cggtttccag
     4201 tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga agttgttttc
     4261 ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag gttttacgaa
     4321 atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact catcatactt
     4381 caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat gtaagctcct
     4441 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat gaaaaaatgc
     4501 gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt ttttacttct
     4561 tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg aattcgagga
     4621 atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac aggtcgattg
     4681 ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg atagcgttag
     4741 ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc ggaccgttcc
     4801 acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa gcatcaccga
     4861 tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta gccgatgcag
     4921 tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg caggtggtca
     4981 gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc acatcgtggc
     5041 cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg tcagcgattt
     5101 tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca acgatagcca
     5161 ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca gctgccaaca
     5221 gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa gcctgcgcgc
     5281 ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg tattttttgg
     5341 acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc agaacaacca
     5401 gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga accgggttag
     5461 acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg ttgtactcgc
     5521 cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt ttcagggcca
     5581 gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc ccgtctttta
     5641 ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc agatcggtca
     5701 tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca gacgctgctt
     5761 gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat tcggttagca
     5821 aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc actgacccgg
     5881 gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac agagaccaac
     5941 ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt ttgcagcaac
     6001 aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc gattgccgtc
     6061 acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa ttgcggaaaa
     6121 aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg gctggctgcc
     6181 cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg catggcggag
     6241 tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa tttgatgacg
     6301 ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc cgcacgaagt
     6361 tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc aggatgtgaa
     6421 aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg ggagccatat
     6481 acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag cgattgcagt
     6541 gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc gcgttcctgt
     6601 cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg cgccatgccc
     6661 gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg tccggattca
     6721 tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc tacgcgtacg
     6781 gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt aatccagcaa
     6841 atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt gtcgaacatt
     6901 taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg aatgattgtc
     6961 cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc ctgtggaatt
     7021 tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt gccgtcacaa
     7081 accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca atacgtccga
     7141 gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg cacgctttta
     7201 tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt ttgcacagca
     7261 gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg acgtcgcctt
     7321 gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag tgatctcact
     7381 aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg atccacacgg
     7441 cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc ttgaactggt
     7501 gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt ctggttacga
     7561 cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt atggcgcgta
     7621 gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc aatatgatcg
     7681 gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc ggcacactta
     7741 ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg accaatttac
     7801 tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg atggtcatcg
     7861 gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca aaagtcgttg
     7921 tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg cgtactgcgc
     7981 tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg cactcgatgg
     8041 ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg gtcgataaaa
     8101 ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc accacgcgcc
     8161 attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta acactgtggg
     8221 cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg atctatagcg
     8281 cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcgggca aaatatgtcg
     8341 cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc tggctaacag
     8401 aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt acgctctggt
     8461 tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc cccgtgcaat
     8521 gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat ctgtttgccg
     8581 gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat gaagcgcagc
     8641 gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg atgcccgcgt
     8701 taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt gcggcgctgg
     8761 atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca gccgatagca
     8821 ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa ttcggagcgt
     8881 ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc atcctccgtt
     8941 ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg acaacggcag
     9001 tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc tgccaggaga
     9061 gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc ttaatgaaca
     9121 atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg gctgtacctt
     9181 tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg aaacagagat
     9241 tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc gccatcgtca
     9301 cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg ccatcgcgat
     9361 gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc aggccagcga
     9421 aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga ttattctttt
     9481 tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat tgcaaaacgg
     9541 agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc tagcgcagca
     9601 acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt ggcgctgacc
     9661 ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct tcttacgccc
     9721 acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa ccgccagcgc
     9781 gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca cagcaccgtc
     9841 gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga aatcactcaa
     9901 cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc gcacccgttg
     9961 gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc actctctccg
    10021 ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt gagcgtgttt
    10081 gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat cagtcatgac
    10141 gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg tgtaatcagg
    10201 aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc aaatttgcag
    10261 gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca taaa
//
LOCUS       DAJTVE010000140        10219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000140 DAJTVE010000000
VERSION     DAJTVE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO439_003445"
     CDS             complement(<1..784)
                     /locus_tag="OO439_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317727.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPESGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO439_003446"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO439_003446"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6317728.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO439_003447"
     CDS             1860..2747
                     /locus_tag="OO439_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6317729.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO439_003448"
     CDS             2780..3790
                     /locus_tag="OO439_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6317730.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO439_003449"
     CDS             3860..5293
                     /locus_tag="OO439_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6317731.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO439_003450"
     CDS             5392..6741
                     /locus_tag="OO439_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6317732.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO439_003451"
     CDS             6731..7618
                     /locus_tag="OO439_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6317733.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO439_003452"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO439_003452"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6317734.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO439_003453"
     CDS             8352..9224
                     /locus_tag="OO439_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6317735.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO439_003454"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO439_003454"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6317736.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..>10219
                     /gene="fabY"
                     /locus_tag="OO439_003455"
                     /gene_synonym="yiiD"
     CDS             9703..>10219
                     /gene="fabY"
                     /locus_tag="OO439_003455"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6317737.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLS"
BASE COUNT         2354 a         2413 c         2714 g         2738 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgctctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcg
//
LOCUS       DAJTVE010000141        10206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000141 DAJTVE010000000
VERSION     DAJTVE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(31..1035)
                     /gene="cra"
                     /locus_tag="OO439_003456"
     CDS             complement(31..1035)
                     /gene="cra"
                     /locus_tag="OO439_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6317738.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1117..1203)
                     /locus_tag="OO439_003457"
     CDS             complement(1117..1203)
                     /locus_tag="OO439_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6317739.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1215..1706)
                     /gene="ilvN"
                     /locus_tag="OO439_003458"
     CDS             complement(1215..1706)
                     /gene="ilvN"
                     /locus_tag="OO439_003458"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6317740.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1709..3433)
                     /gene="ilvI"
                     /locus_tag="OO439_003459"
     CDS             complement(1709..3433)
                     /gene="ilvI"
                     /locus_tag="OO439_003459"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6317741.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3552..3596
                     /locus_tag="OO439_003460"
     CDS             3552..3596
                     /locus_tag="OO439_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317742.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3751..4695)
                     /gene="leuO"
                     /locus_tag="OO439_003461"
     CDS             complement(3751..4695)
                     /gene="leuO"
                     /locus_tag="OO439_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6317743.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5355..5441
                     /gene="leuL"
                     /locus_tag="OO439_003462"
     CDS             5355..5441
                     /gene="leuL"
                     /locus_tag="OO439_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6317744.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5534..7105
                     /gene="leuA"
                     /locus_tag="OO439_003463"
     CDS             5534..7105
                     /gene="leuA"
                     /locus_tag="OO439_003463"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6317745.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7105..8196
                     /gene="leuB"
                     /locus_tag="OO439_003464"
     CDS             7105..8196
                     /gene="leuB"
                     /locus_tag="OO439_003464"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6317746.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8199..9599
                     /gene="leuC"
                     /locus_tag="OO439_003465"
     CDS             8199..9599
                     /gene="leuC"
                     /locus_tag="OO439_003465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6317747.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9610..>10206
                     /gene="leuD"
                     /locus_tag="OO439_003466"
     CDS             9610..>10206
                     /gene="leuD"
                     /locus_tag="OO439_003466"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6317748.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAF"
BASE COUNT         2669 a         2681 c         2498 g         2358 t
ORIGIN      
        1 attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcggcgata
       61 gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc
      121 caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac
      181 cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga
      241 aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga
      301 cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc
      361 agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt
      421 ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc
      481 aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc
      541 atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc ggtccagcgc
      601 gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gaggcaacga
      661 cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac agcgcatttc
      721 gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt gccgcgcctg
      781 gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag
      841 accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt
      901 gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt
      961 cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat
     1021 ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg
     1081 tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac tgcttttatt
     1141 ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg
     1201 cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagaa
     1261 cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc atcaagctta
     1321 tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat aatttgccca
     1381 cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc
     1441 accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca
     1501 tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc gcccacggtc
     1561 tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag gctttcaatg
     1621 ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg
     1681 agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt
     1741 catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca tcgacggtaa
     1801 catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta agtttgcttt
     1861 ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc gccagacgga
     1921 cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct
     1981 gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt acgggcaact
     2041 cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga
     2101 cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa
     2161 aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat
     2221 aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg
     2281 tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat
     2341 atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg cggatctcat
     2401 ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg aggacctggc
     2461 gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa gtaggatcaa
     2521 tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc gtcgttcggt
     2581 catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt
     2641 cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc ggaaacgctc
     2701 ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact
     2761 gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt ttttttgccg
     2821 ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta gtgggattgt
     2881 aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc ggattaagaa
     2941 tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc agccagaaag
     3001 cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca
     3061 ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg
     3121 tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc
     3181 cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg
     3241 tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga actaaaacat
     3301 gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat
     3361 aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag
     3421 acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc
     3481 gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa
     3541 caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgataat
     3601 aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaat
     3661 agagagccca cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag
     3721 acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc
     3781 ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt
     3841 tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc
     3901 ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac
     3961 gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca
     4021 aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac
     4081 atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat
     4141 ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat
     4201 cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt
     4261 gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag
     4321 aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc
     4381 aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa
     4441 aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc
     4501 attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag
     4561 aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa
     4621 gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt
     4681 ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc ttattggaat
     4741 gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa
     4801 tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt
     4861 ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga aaacaatcta
     4921 atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc ttatttaata
     4981 attaatttat atatttaatg cattaattct taacattaat tgatcaataa tattcaccaa
     5041 atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag gataggtttt
     5101 gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat ttatgctgaa
     5161 atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc ttactttaac
     5221 caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg gacgttttta
     5281 ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgcattcat
     5341 ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat
     5401 cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca
     5461 aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac
     5521 aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga
     5581 acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga
     5641 gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg gcgattttga
     5701 atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg
     5761 cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat
     5821 tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca gcaccctgga
     5881 cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt
     5941 tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga
     6001 agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat
     6061 gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa
     6121 agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca actcactggc
     6181 ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc
     6241 cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa
     6301 cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag ttagccagat
     6361 ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat tcgctcactc
     6421 ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc
     6481 agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc
     6541 ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt tagacaattt
     6601 gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga
     6661 ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt
     6721 cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgtggcga
     6781 agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcaat
     6841 taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca
     6901 cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc gccgcttcca
     6961 cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact
     7021 gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag ctcaacacaa
     7081 cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccgggg
     7141 gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac
     7201 cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc cattgataac
     7261 cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg
     7321 tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca accagaacgc
     7381 ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg cccggcaaaa
     7441 ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc taacggcttc
     7501 gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc
     7561 gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc
     7621 gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa agtcacctca
     7681 atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc
     7741 gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc caccatgcag
     7801 ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt
     7861 ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc cgccagcctg
     7921 aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc
     7981 aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc
     8041 ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa
     8101 ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc
     8161 gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa
     8221 aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg
     8281 accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg cgcgcccacg
     8341 gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac gtctctaccc
     8401 agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta
     8461 aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat caggggatcg
     8521 tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt gtctgcggcg
     8581 actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg
     8641 aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa accatgaaaa
     8701 ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta
     8761 tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa
     8821 tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg
     8881 caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa ggccgtttgc
     8941 atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg
     9001 acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt tcaccgcagg
     9061 tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt cccgatccgg
     9121 cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc tatatggggc
     9181 tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgta
     9241 ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg
     9301 cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc caggcggaag
     9361 cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg cctggctgct
     9421 caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcatccacca
     9481 gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg
     9541 caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac attaaataag
     9601 gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc
     9661 gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtacg
     9721 ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg ccaacagcca
     9781 aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt gctggcacga
     9841 gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggc
     9901 tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac
     9961 cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc gctggtgaaa
    10021 gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa agcgggagag
    10081 aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa cggtctggac
    10141 agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc aaaacaacct
    10201 gcgttt
//
LOCUS       DAJTVE010000142         9863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000142 DAJTVE010000000
VERSION     DAJTVE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO439_003467"
     CDS             486..1157
                     /locus_tag="OO439_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6317749.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO439_003468"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO439_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO439_003469"
     CDS             2363..2923
                     /locus_tag="OO439_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6317750.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO439_003470"
     CDS             2925..3698
                     /locus_tag="OO439_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6317751.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO439_003471"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO439_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6317752.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO439_003472"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO439_003472"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6317753.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO439_003473"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO439_003473"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6317754.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO439_003474"
     CDS             complement(6457..6996)
                     /locus_tag="OO439_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6317755.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO439_003475"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO439_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6317756.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO439_003476"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO439_003476"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6317757.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..>9863)
                     /gene="mhpF"
                     /locus_tag="OO439_003477"
     CDS             complement(9606..>9863)
                     /gene="mhpF"
                     /locus_tag="OO439_003477"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6317758.1"
                     /translation="AVQAHVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLE
                     VEGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
BASE COUNT         2451 a         2583 c         2398 g         2431 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt gagcctgcac cgc
//
LOCUS       DAJTVE010000143         9833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000143 DAJTVE010000000
VERSION     DAJTVE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(374..1351)
                     /gene="epmA"
                     /locus_tag="OO439_003478"
     CDS             complement(374..1351)
                     /gene="epmA"
                     /locus_tag="OO439_003478"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6317759.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1676..3484
                     /gene="frdA"
                     /locus_tag="OO439_003479"
     CDS             1676..3484
                     /gene="frdA"
                     /locus_tag="OO439_003479"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6317760.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3477..4211
                     /gene="frdB"
                     /locus_tag="OO439_003480"
     CDS             3477..4211
                     /gene="frdB"
                     /locus_tag="OO439_003480"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6317761.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4222..4617
                     /gene="frdC"
                     /locus_tag="OO439_003481"
     CDS             4222..4617
                     /gene="frdC"
                     /locus_tag="OO439_003481"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6317762.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4628..4987
                     /gene="frdD"
                     /locus_tag="OO439_003482"
     CDS             4628..4987
                     /gene="frdD"
                     /locus_tag="OO439_003482"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6317763.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5050..6183
                     /locus_tag="OO439_003483"
     CDS             5050..6183
                     /locus_tag="OO439_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6317764.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6272..6805
                     /gene="blc"
                     /locus_tag="OO439_003484"
     CDS             6272..6805
                     /gene="blc"
                     /locus_tag="OO439_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6317765.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6802..7119)
                     /gene="sugE"
                     /locus_tag="OO439_003485"
     CDS             complement(6802..7119)
                     /gene="sugE"
                     /locus_tag="OO439_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6317766.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7294..7440)
                     /gene="ecnB"
                     /locus_tag="OO439_003486"
     CDS             complement(7294..7440)
                     /gene="ecnB"
                     /locus_tag="OO439_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6317767.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7551..7676)
                     /gene="ecnA"
                     /locus_tag="OO439_003487"
     CDS             complement(7551..7676)
                     /gene="ecnA"
                     /locus_tag="OO439_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6317768.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7728..8294)
                     /gene="efp"
                     /locus_tag="OO439_003488"
     CDS             complement(7728..8294)
                     /gene="efp"
                     /locus_tag="OO439_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6317769.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8336..9364
                     /gene="epmB"
                     /locus_tag="OO439_003489"
     CDS             8336..9364
                     /gene="epmB"
                     /locus_tag="OO439_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6317770.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9754..>9833
                     /locus_tag="OO439_003490"
     CDS             9754..>9833
                     /locus_tag="OO439_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317771.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKI"
BASE COUNT         2511 a         2499 c         2497 g         2326 t
ORIGIN      
        1 ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag
       61 taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt
      121 cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt
      181 gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa
      241 tacagaattg atacccggat gatatcaaat atccagtctt aaattgtggc ctttaacgga
      301 aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag
      361 taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg
      421 cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca
      481 taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg
      541 ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt
      601 catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc
      661 gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa
      721 aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca
      781 acgtgtcgcg gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt
      841 cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt
      901 gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca
      961 cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact
     1021 cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg
     1081 gcccacaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga
     1141 gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa
     1201 tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg
     1261 caaagaaacg acggatctcc gccataatcg ccgcacgttt taataagtta ggaatggatg
     1321 cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca
     1381 cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg
     1441 gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga
     1501 taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc
     1561 agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc
     1621 aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca
     1681 aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat
     1741 tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat
     1801 gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag
     1861 cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt
     1921 cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg
     1981 cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat
     2041 cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca
     2101 gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct
     2161 ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt
     2221 gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta
     2281 caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt
     2341 tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat
     2401 cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg
     2461 ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata
     2521 tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg
     2581 caacaccatc tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga
     2641 gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt
     2701 cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat
     2761 cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc
     2821 ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt
     2881 cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa
     2941 cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa
     3001 ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga
     3061 aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga
     3121 gctgcaggaa tgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga
     3181 cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc
     3241 cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga
     3301 gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac
     3361 tcgcctagag tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg
     3421 tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg
     3481 ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac
     3541 cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg
     3601 tctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga
     3661 tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc
     3721 tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg
     3781 atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca
     3841 tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa
     3901 agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt
     3961 ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg
     4021 aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg
     4081 tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg
     4141 ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga
     4201 aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg
     4261 tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg
     4321 gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc
     4381 ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac
     4441 ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa
     4501 gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc
     4561 tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga
     4621 gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt
     4681 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat
     4741 tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt
     4801 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
     4861 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa
     4921 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac
     4981 aatctaacgt atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
     5041 ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca
     5101 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
     5161 caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
     5221 acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
     5281 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
     5341 gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttactgc taaacagtgg
     5401 aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg
     5461 ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
     5521 tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
     5581 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca
     5641 ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg
     5701 ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat
     5761 ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt
     5821 gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa
     5881 accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac
     5941 agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt
     6001 aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga
     6061 tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac
     6121 aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag
     6181 taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta
     6241 cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc
     6301 gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt
     6361 agtgaataat ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga
     6421 tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatgacgg
     6481 cggcctgaat gtcatcaata aaggctataa ccctgacaga gaaatgtggc agcagagtga
     6541 agggaaagcg tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg
     6601 tcctttctat ggcggttata acgtcattgc acttgatcgg gaataccgcc atgcgctggt
     6661 ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga
     6721 agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat
     6781 ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac
     6841 gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc
     6901 ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg
     6961 taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg tcacagtaat
     7021 aacacttggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac
     7081 ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg
     7141 gccatccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga
     7201 tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg caaaaaaaca
     7261 aacggcacga cataactgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcacc
     7321 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7381 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7441 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7501 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7561 gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg agcggtgtta
     7621 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7681 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7741 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7801 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7861 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7921 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     7981 ttagcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8041 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8101 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8161 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8221 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8281 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8341 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8401 tgtgaccgat cctggtgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8461 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8521 catggagaaa ggcaatccgg acgatcctat tttgcgtcag gtacttacct cgcaagatga
     8581 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8641 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8701 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8761 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8821 ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc tcacacaact
     8881 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     8941 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9001 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9061 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9121 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9181 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9241 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9301 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9361 gtaactgatt aaacagggaa gttaaacatt agcctgatgc gctacgctta tcaggcctac
     9421 attgtgcatt gaaattgaat ggatttgcaa tgaactgtag gccggataag gcgtttacgc
     9481 cgcatccggc aagagcaaca aacgcaagta cagcaacctg tcattaaact gggaaattta
     9541 atctttgccc gttgcataac ctttaattat tcatatacat tgctgttgtt actctttata
     9601 tagccaacac caataattaa cctttctttt ctgtttatat agagtgagta cggctattta
     9661 tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat taaaattaat
     9721 tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa gggtgttaat
     9781 accggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat taa
//
LOCUS       DAJTVE010000144         9810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000144 DAJTVE010000000
VERSION     DAJTVE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO439_003491"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO439_003491"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6317772.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO439_003492"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO439_003492"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6317773.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO439_003493"
     CDS             1829..2101
                     /locus_tag="OO439_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6317774.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO439_003494"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO439_003494"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6317775.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO439_003495"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO439_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6317776.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO439_003496"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO439_003496"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6317777.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO439_003497"
     CDS             6114..6665
                     /locus_tag="OO439_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6317778.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO439_003498"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO439_003498"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6317779.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO439_003499"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO439_003499"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6317780.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2432 a         2449 c         2615 g         2314 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc
//
LOCUS       DAJTVE010000145         9644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000145 DAJTVE010000000
VERSION     DAJTVE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..667)
                     /locus_tag="OO439_003500"
     CDS             complement(<1..667)
                     /locus_tag="OO439_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6317781.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO439_003501"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO439_003501"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6317782.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="OO439_003502"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="OO439_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6317783.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="OO439_003503"
     CDS             complement(5698..7014)
                     /locus_tag="OO439_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6317784.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="OO439_003504"
     CDS             complement(7004..8761)
                     /locus_tag="OO439_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6317785.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="OO439_003505"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="OO439_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAJTVE010000146         9541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000146 DAJTVE010000000
VERSION     DAJTVE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1320
                     /locus_tag="OO439_003506"
     CDS             <1..1320
                     /locus_tag="OO439_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6317786.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1323..2651
                     /gene="ssnA"
                     /locus_tag="OO439_003507"
     CDS             1323..2651
                     /gene="ssnA"
                     /locus_tag="OO439_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6317787.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2702..3481
                     /locus_tag="OO439_003508"
     CDS             2702..3481
                     /locus_tag="OO439_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6317788.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3478..6417
                     /locus_tag="OO439_003509"
     CDS             3478..6417
                     /locus_tag="OO439_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6317789.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6350..6664
                     /locus_tag="OO439_003510"
     CDS             6350..6664
                     /locus_tag="OO439_003510"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6317790.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6700..7050
                     /gene="ghxQ"
                     /locus_tag="OO439_003511"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6700..7050
                     /gene="ghxQ"
                     /locus_tag="OO439_003511"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7119..7607)
                     /locus_tag="OO439_003512"
     CDS             complement(7119..7607)
                     /locus_tag="OO439_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6317791.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7607..9526)
                     /gene="ygfT"
                     /locus_tag="OO439_003513"
     CDS             complement(7607..9526)
                     /gene="ygfT"
                     /locus_tag="OO439_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6317792.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2193 t
ORIGIN      
        1 cctcagttcc gtattcctgc agagttaatt cagcacgata tcgattttgt tgccgctcac
       61 ggcgtgaaat ttgagtatgg ctgctcaccc gatttaaccg ttgaacagtt aaaaaatcag
      121 ggcttccact atgttctgat tgccaccggc actgataaaa atagcggtgt gaaactggcg
      181 ggcgacaacc aaaatgtctg gaaatcactc cccttcctgc gtgaatacaa caagggtaca
      241 gcgctcaagc tgggcaaaca tgtggtcgtt gtcggggcgg gtaacaccgc aatggactgc
      301 gctcgtgcgg cgttacgcgt tccaggcgta gaaaaagcaa cgatcgttta ccgtcgttca
      361 ctacaagaga tgcccgcatg gcgcgaagag tatgaagaag cgttgcacga cggcgtagag
      421 ttccgtttcc tgaataatcc ggaacgtttc gatgctgatg gcaccttaac cttgcgcgtt
      481 atgtcgcttg gcgaaccgga tgagaaaggt cgtcgccgtc cggttgaaac caacgaaaca
      541 gtaacactgc atgtagacag cctgatcacc gccattggtg aacagcagga tactgaagcc
      601 ctgaatgcga tgggcgtgcc gctggacaaa aacggctggc cagacgtcga ccataatggc
      661 gaaactcgtc tgactgacgt ctttatgatc ggcgacgtac agcgcggacc atcctccatt
      721 gtcgctgctg tcggaaccgc gcgtcgggcg accgatgcca tccttagtcg ggaaaatatc
      781 cgttcccacc agaacgataa atactggaac aacgtcaatc cagcggaaat ctatcaacgt
      841 aaaggcgata tctctatcac gctggtgaac agtgacgatc gtgacgcgtt tgtcgcccag
      901 gaagccgctc gctgcctcga atgtaactac gtttgcaaca agtgtgtgga tgtctgcccg
      961 aaccgcgcca acgtctccat tgcagtccct ggcttccaga atagattcca gacgctgcac
     1021 ctcgacgctt actgtaacga atgcggcaac tgcgctcagt tctgtccgtg gaacggtaaa
     1081 ccatacgaag acaaaatcac cgtcttcagc ctggcgcaag actttgataa cagcagcacc
     1141 ccaggcttcc ttgtggaaga ttgccgggta cgagtacgtc tgaataacca aagctgggtg
     1201 ttaaacatcg acagcgaagg tcagtttaac aacgtaccac cggagctgaa cgatatgtgc
     1261 cgcatcatca gccatgtcca ccagcatcat cattatctgc tgggccgcgt ggaggtgtaa
     1321 tcatgttgat tctgaagaat gtcaccgcag tgcagttaca cccggcgaaa gtgcaggaag
     1381 gcgttgatat cgctatcgaa aatgacgtga ttgtcgctat cggcgatgcc ctgacgcaac
     1441 gctaccccga tgccagctac aaagagatgc atggtcggat tgtgatgccg ggaattgtct
     1501 gctcgcacaa ccatttttac tcggagcttt cccgcggaat tatggcaaac atcgcccctt
     1561 gcccggattt catctcaacg ctgaaaaatc tctggtggcg gctcgatcgc gcccttgatg
     1621 aagagtcgct ctattacagc ggactgattt gttccctgga agcgattaag agcggatgta
     1681 catcggttat cgatcaccat gcctcgccgg cgtatatcgg cgggtcgctc tccacattgc
     1741 gcgacgcatt tttaaaagtt ggcctgcgcg cgatgacctg ttttgaaact actgaccgta
     1801 acaacggcat caaagagttg caggaaggtg tagaagaaaa catccgtttc gcccgtttga
     1861 ttgatgaggc gaagaaagcg acaagcgagc cgtatctggt ggaagcacat atcggtgctc
     1921 acgcgccgtt taccgtgccg gatgccggtc tggagatgct gcgtgaagcc gtgaaagcca
     1981 caggccgtgg tttgcatatt cacgctgcgg aagaccttta cgacgtttcc tacagtcacc
     2041 actggtacgg caaagacctg ctggcacgac tggcgcaatt cgacctcatc gacagcaaaa
     2101 cgctggtcgc tcatgggctg tacttgtcga aagatgacat cgccctactc aatcagcgcg
     2161 atgcgttcct ggtgcataac gcccgttcaa acatgaacaa ccatgtcggc tacaaccatc
     2221 accttagcga catccgcaac ctggcattgg gaacggacgg cattggttcg gacatgtttg
     2281 aagagatgaa atttgccttc tttaaacatc gcgatgcggg tggtccgctg tggcctgaca
     2341 gttttgccaa agccctgact aacggtaacg aactgatgag ccgcaacttt ggcgcgaaat
     2401 ttgggcttct ggaagccggt tacaaagctg atttgaccat ttgcgattac aactcgccga
     2461 cgccgctgct ggcagacaat atcgccgggc atatcgcttt cggtatgggc tcaggcagcg
     2521 ttcacagcgt gatggtcaat ggtgtgatgg tctatgaaga ccgtcagttt aacttcgatt
     2581 gcgattccat ttatgcacaa gccagaaaag ccgctgccag tatgtggcgt cggatggatg
     2641 cgctggcata aatgacagat gccctcttcc cgcagggaag agggttaaat aaggaaagaa
     2701 gatgattgaa caatttttca ggcccgactc tgtcgaacag gcgctggaac tgaagcgccg
     2761 ctaccaggat gaagccgtct ggttcgccgg gggcagcaaa ctcaatgcta caccaacccg
     2821 taccgataaa aagattgcca tttccttaca ggatctggaa ctggactggg ttgactggga
     2881 taacggtgca ctgcggattg gtgcaatgtc tcgcttgcag ccactgcgtg atgcgcgctt
     2941 tattcctgca gcactgcgtg aagccctcgg ttttgtttac tcacgccatg ttcgtaatca
     3001 gtcgaccatt ggtggtgaaa tcgccgcccg gcaggaagag tcagtgctgc ttcccgtcct
     3061 gctagcactg gatgctgaac tggtttttgg caacggcgaa acgctgtcaa tcgaggacta
     3121 cctggcctgc ccatgcgatc gcctgctaac cgaaattatc attaaagatc cgtatcgcac
     3181 ctgtgcgacc cgcaaaatta gccgttctca ggcaggttta accgtcgtga cggcagccgt
     3241 tgcaatgaca gaccacgacg gtatgcgaat tgcgctggat ggcgtcgcca gtaaagcact
     3301 gcgtctgcac gatgtcgaaa aacaaaatct ggaaggcaat gcacttgaac aggctgtcgc
     3361 caacgccatt ttcccgcagg aagatttgcg gggcagcgtg gcctataaac gctatatcac
     3421 gggagttctg gtagccgacc tgtatgccga ctgccaacag gctggggagg aagccgtatg
     3481 atcatccact ttactttaaa tggcgcgcct caggagctaa ccgttaatcc aggcgaaaac
     3541 gtgcaaaagc tgttgtttaa catgggaatg cactctgtac gcaacagtga tgatggcttc
     3601 gggtttgccg gttctgacgc aataatcttt aacggtaata tcgttaacgc gtccttgctt
     3661 attgccgcac agttagagaa ggcagatatt cgtaccgcag aatctctggg taaatggaac
     3721 gagttaagtc tggttcaaca ggcaatggtt gatgttggcg tggtgcagtc tggttataac
     3781 gatccagctg cagctctgat tatcaccgat cttctcgatc gcatcgccgc acctacccgc
     3841 gaagagatcg acgacgcgct ttctggtttg ttcagccgcg atgcaggctg gcaacaatac
     3901 tatcaggtca ttgaactggc ggttgcacgt aaaaataatc cgcaggccac cattgatatc
     3961 gctccgactt tccgtgacga ccttgacgtc attggcaagc attatcctaa aactgatgcc
     4021 gcgaaaatgg tgcaggcgaa accctgctat gttgaagact gcgtaacggc tgacgcctgc
     4081 gtcattaaaa tgttacgtag cccacacgct cacgcactga ttactcatct ggatgtcagc
     4141 aaagctgaag ccttaccggg cgtcgttcac gttattactc acctgaattg ccctgatatc
     4201 tactataccc cggggggtca gagcgcaccg gaaccgtcac cgcttgaccg ccgtatgttc
     4261 ggcaagaaaa tgcgtcacgt cggcgatcgt gttgctgcgg tcgtcgcaga aagtgaagaa
     4321 attgcgctcg aagcactgaa gctcatcgac gttgaatatg aagtgcttaa gccggtaatg
     4381 tcgatcgacg aagcaatggc ggaagatgcg cctgtcgtgc acgatgaacc ggtggtgtat
     4441 gttgctggtg cgccagatac tctggaagac gataacagcc atgcagccca gcgcggcgag
     4501 catatgatca tcaacttccc gatcggttct cgccctcgca aaaatatcgc cgccagtatt
     4561 catggtcata ttggcgatat ggacaaaggc tttgccgatg ccgatgtgat cattgagcga
     4621 acctataact caacgcaggc acagcagtgc ccgactgaaa cacatatctg ctttacccgg
     4681 atggacggcg atcgtctggt tatccacgcc tccacccagg taccatggca cttacgccgc
     4741 caggtcgcgc gcctcgtggg catgaaacag cataaagttc atgtcattaa agagcgagtt
     4801 ggcggcggtt ttggttccaa acaggacatc ctgctggaag aagtgtgcgc ctgggcaacc
     4861 tgcgtgaccg ggcgtccggt actattccgc tacacccgtg aagaagagtt tattgctaac
     4921 acctctcgtc acgtcgcgaa agtcaccgtc aaactgggag cgaaaaaaga tggtcgcctg
     4981 acggcagtga agatggattt ccgcgccaac actggccctt acggcaacca ctcactcacc
     5041 gtaccgtgta acggaccggc gctgtcgctg ccgttatatc cgtgcgataa cgtcgatttc
     5101 caggtcacca cctactacag caacatttgc ccaaatggtg cttatcaggg ttatggcgca
     5161 ccgaaaggta acttcgctat caccatggca ttagcggaac tggctgaaca gttacagatc
     5221 gaccaactgg aaattatcga acgtaaccgg gtacacgaag ggcaagagct gaaaattctc
     5281 ggtgcaatcg gtgaaggtaa agcgccgacc tccgttcctt ccgccgccag ctgtgcactg
     5341 gaagagatcc tgcgtcaggg tcgcgagatg atccaatggt cgtcaccgaa accgcaaaat
     5401 ggtgactggc acatcggtcg cggcgtcgcc attatcatgc agaaatcggg tatcccggat
     5461 atcgatcagg ctaactgcat gatcaaactg gaatcggacg gcacctttat cgttcattct
     5521 ggcggtgcgg atattggtac tggtctggat actgtagtga cgaaactggc agcagaagtg
     5581 ctgcactgcc caccgcagga cgtgcatgtt atctccggtg ataccgatca tgcgttgttt
     5641 gataaaggcg catatgcctc gtccggtact tgcttctcgg gtaacgcggc gcgtttggca
     5701 gcggaaaatc tacgggagaa aattctgttc cacggcgcgc aaatgttggg tgaggcagtg
     5761 gcagatgttc aactggcaac gccgggcgtc gtgcgcggca agaaaggcga agttagtttc
     5821 ggggatattg cccataaagg cgaaaccggc atcggctttg gttcactggt gggaactggc
     5881 agttatatca cgcctgattt cgccttcccg tatggcgcaa acttcgctga agttgccgtc
     5941 aacacgcgta cgggtgaaat ccgcctggat aaattctacg ccttgctgga ctgcggtaca
     6001 ccggtcaacc cggaattagc attgggacaa atctacggtg ctaccctgcg agctatcggc
     6061 cacagtatga gcgaagagat catttatgac gccgaaggtc acccgttaac gcgtgattta
     6121 cgcagttacg gcgcaccgaa aattggtgac attccgcgtg atttccgcgc tgtgctggtg
     6181 ccgagcgacg ataaagtcgg cccgttcggg gcgaaatcga tctcggaaat cggtgtaaat
     6241 ggcgcagctc cggcgattgc taccgcaatt cacgatacat gcggcatctg ggcagaagaa
     6301 agtcaaagtg ggcatgggaa cggatatcgg tgccggaaca actttcaaca tgctgcaaac
     6361 gctgaacgaa gcctacaaag tgttgcaatt acaaggctat cgcctctcgg catatgaagc
     6421 gttttacctg gccacgctcg gcggagcgaa atctctgggc cttgacgatt tgattggcaa
     6481 ctttttacct ggcaaagagg ctgatttcgt ggtgatggaa cccaccgcca ctccgctaca
     6541 gcagctgcgc tatgacaact ctgtttcttt agtcgacaaa ttgttcgtga tgatgacgtt
     6601 gggcgatgac cgttcgatct accgcaccta cgttgatggt cgtctggtgt acgaacgcaa
     6661 ctaataataa aactttaaca tcctcgtgag gacatcatta tgtctggaga catcctacaa
     6721 acaccggacg caccaaagcc acagggcgcg ctggataatt attttaaaat taccgctcgt
     6781 ggcagtaccg ttcgtcagga agtactggct ggcttaacga cctttctggc catggtttat
     6841 tccgttatcg tcgttccggg aatgctgggc aaagcagttt ttcctcccgc agctgtgttt
     6901 gttgccacct gtctggtcgc gggcttcggc tcgttgctga tggggttgta ggccaatttg
     6961 ccaatggcga ttggttgtgc gatttccttg acggcattta ccgcattcag tctggtactc
     7021 gggcaacaaa ttagcgttcc tgtcgcactg gtcgcatttt acgcgggtgg ttgggcaatc
     7081 taatagattc tccgcgcctt tcagcgcgga gatttctttc acagaggatt ctcaccgctg
     7141 gcaacaatac gctgctggcg gactcgcctg agttctcttt cagtaagcaa ctgcagcgcc
     7201 tgtgtcgggc aagattcaac acaggctggc ccctcttccc gctgttcaca gagatcgcat
     7261 ttcacaattt gttgtcgggt atcccccggc aacgactgaa tggtgatcat tccaaacggg
     7321 cacgcactga cgcaactctg acagccaata caacgggcag aattggtctg caccacctgc
     7381 tcgcccatcg tcagcgcccc cacggggcaa gcgccaacac aaggggcgtt ttcacactga
     7441 tggcacatca ccggcgcact gatgctgtcc agtcgctgta ccttcagccg gggtaaaaag
     7501 acatcggcat tcaactcctg ttctgaaggg tgagcgacca cacaggctac ttcacaagta
     7561 cggcagccaa tacagtcagc cggattaacg ataattaacg atttcatcac gatgccttcg
     7621 tatcaaacag agttaacata tcgcgcgccg cctgccttcc tgcggccatt gcagtgacaa
     7681 ccagatccgc gccatgaact gcatcaccac cagcaaagac ttttttcaga tgcgtctggg
     7741 taggtaaata cccgacgtca ccggtttgaa tcaggcccca tttatcgagt ttaattccgc
     7801 tgccctgcaa ccacggcatg gcatgcgcct ggaaaccaaa ggccataatc agaacatcgg
     7861 cgggcaattc aaactctgaa cccgctaccg gacgaggacg acggcgacca tccggccccg
     7921 gctcacccat ggcggtacga atcaggccca ccgcagttaa acgcccatct tcgtcacaag
     7981 cgatatattg cggttgaaca ttgaactgaa actcgacacc ttcctcgcgc gcattaacca
     8041 cctctttgcg cgagcccggc atactgactt catcacgacg atacgcgcag gtcacgctgg
     8101 cagcattgag gcgaatggaa gtccgcaaac aatccattgt cgtatcgcca ccgcccaata
     8161 ccacgactcg cttaccttcc acgtccgtca gcggatactc ttcagactcc ggcaatccca
     8221 tgagctggcg ggtatgggca gtcaggaacg gtagagcctg aatgacaccg ggcgcatctt
     8281 catgcggcag atctgctcgc atcatcccgt aagtccccac gccgatgaaa actgcatcat
     8341 attcagaagt taaatcgcta aaggtaatat cgcggccaat ttcacagtta agatgaaagt
     8401 cgattcccat tgcggtgaat atctctcgcc gctggcttaa taccgtttta tcgagtttga
     8461 aaggaggaat gccaaaagtc agcataccgc caatttctgg atggcgatca aagacatcaa
     8521 cctgaactcc cgcgcgcgcc agaatatcag cacaccctaa ccctgctggc ccagcgccaa
     8581 tcaccgccac tttttcgata cggggaacaa ctttgctgac atcgggacgc cagcccatcg
     8641 ccagcgcggt atcggtgatg tagcgttcca gattaccgat agagactgcg ccagagtgat
     8701 ctttcaaagt acatgcgcct tcacaaagac ggtcctgtgg gcataccctg ccgcagattt
     8761 ctggtaagga actggtctgg tggcaaagtt ctgccgcttc aataatcttt ccttcctgta
     8821 ccagacggat gtaatccgga atagcgttat gcagcgggca atgccagttg cagttagctt
     8881 tttcggcaca ataaacacag cggtcactct cataagtcgc ttgcagtgga tccagcccgc
     8941 aatagatttc gccaaagtgg gttttccgtt cactcgctga aattttatct gcgcctttac
     9001 gcgagttaac ggggagcaac gctgcactgc gagatggctg agcgtctgat gacgcttttc
     9061 ctgctgccgt tttgcgctgg cgagccacct ttatccactg taacccttta tcgtccatca
     9121 gtcgtaacgc ctgtgttggg cagacttcaa tacaggcttg cgtgccggaa ctgcgctggt
     9181 tacaaaggtc gcatttctgc gcaatcgtat cgaccatctc aacgacgcca aaggggcaag
     9241 cgattgcgca tcttttacag ccaatacatt tttgctcgtc cagttgtacg ctatcggact
     9301 ggaaagtcag agcattaacc ggacaagccg taacacaagg ggcattgttg cagtgatggc
     9361 aggccaccgg attcgcagcc tggcctttcc ctacaacgtg gatacgcggt cgaaagtcac
     9421 tgtgactcag cggccagttt tcttgattgt gtgccaccgc acaggcaatt tcacaagcat
     9481 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
     9541 t
//
LOCUS       DAJTVE010000147         9504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000147 DAJTVE010000000
VERSION     DAJTVE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..1233)
                     /locus_tag="OO439_003514"
     CDS             complement(151..1233)
                     /locus_tag="OO439_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6317793.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1230..3026)
                     /locus_tag="OO439_003515"
     CDS             complement(1230..3026)
                     /locus_tag="OO439_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6317794.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3277..3633)
                     /locus_tag="OO439_003516"
     CDS             complement(3277..3633)
                     /locus_tag="OO439_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317795.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3691..4113)
                     /locus_tag="OO439_003517"
     CDS             complement(3691..4113)
                     /locus_tag="OO439_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6317796.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4129..4854)
                     /locus_tag="OO439_003518"
     CDS             complement(4129..4854)
                     /locus_tag="OO439_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6317797.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4876..5622)
                     /locus_tag="OO439_003519"
     CDS             complement(4876..5622)
                     /locus_tag="OO439_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6317798.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5629..6693)
                     /locus_tag="OO439_003520"
     CDS             complement(5629..6693)
                     /locus_tag="OO439_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6317799.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6765..7190)
                     /locus_tag="OO439_003521"
     CDS             complement(6765..7190)
                     /locus_tag="OO439_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6317800.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7187..7450)
                     /locus_tag="OO439_003522"
     CDS             complement(7187..7450)
                     /locus_tag="OO439_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6317801.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7562..8062
                     /locus_tag="OO439_003523"
     CDS             7562..8062
                     /locus_tag="OO439_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6317802.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8082..8276
                     /locus_tag="OO439_003524"
     CDS             8082..8276
                     /locus_tag="OO439_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6317803.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8276..8566
                     /locus_tag="OO439_003525"
     CDS             8276..8566
                     /locus_tag="OO439_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6317804.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8847..8999
                     /locus_tag="OO439_003526"
     CDS             8847..8999
                     /locus_tag="OO439_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6317805.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8999..9376
                     /locus_tag="OO439_003527"
     CDS             8999..9376
                     /locus_tag="OO439_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317806.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2446 a         2493 c         2017 g         2548 t
ORIGIN      
        1 gccacagaac aattattgct tttgccgttg atgacaataa agtttttatc gcaggcgtat
       61 accacggtgg gcaaatctat gaaaccgatt ttttatccag tgcaccgtaa aacccccatc
      121 cttcagggcg tggcggatat caaactttgg tcattttctc tggctgatct ttaccggata
      181 accatcccga agatcaatta agtgctccgg ttcagaagca aaccatgcat aagatcccca
      241 cgccaattct ggtattgcct ttttaaaagg cagagcactt ctgtccataa atgccgtaag
      301 gaatgcaaga tttttctcgc tgaacccagc gtcaatagcc aaatgagtga gagtaacttt
      361 tcgttccctg tttataggcc catctgtcgc aactacttca gtgaaaacta caagcatgtc
      421 cgaacccgtt cggtcctctc ccaaatcaat caaaataata tctggaagtg cttttgaggc
      481 atcaatattc agccccaatg cttttgccaa tacatcatcc tgagcaacaa ctttattccc
      541 ggattcagac aaccagagca cagttggatt cttaagaaaa ttaggagcaa aaacttcgat
      601 gacggcctta gctattgtac ttgacgggcc cggagctaaa gttctcttct ctccatttgg
      661 gaaagtgacg actacggcat cagcagcaat cactgcccct gatttcaata aacgcaacct
      721 agcaagagcg gctttgttca aatgattttc ttgccaggaa cttattgcac tcagcagcga
      781 atctccttca agcccggcat caaagagtgc tgcaaaagag cgattcaaac tgtaacgagg
      841 acgagaggat gtggtgggta tacctttacg ctcaacaatt gcattgcaag gtattaggcc
      901 attttttata gtttcatcac gtattggctc acgcgtattt tccgcatacc atgcatcagc
      961 aggccttatc ttttgtttag agaaagtcat ctttacccag ctttctctgg acgcatcatc
     1021 agtaagagca gcctgcgaat ctcccatact tacaacctgg ctgggcctta tccatcgccc
     1081 cagcccctca accgcaccag cataaaacat gacaaaaatt gtttttgcag ctatttcccg
     1141 aattaaatat ccacgattct cagtcccttc agggaaaatt aattgtaatc tttcccggat
     1201 aacattcaat gggggaacaa caggcagatt catgcaatca tcactccata gaaaccagcg
     1261 atagttctct caataatccg tctgtggacg ccagcatcaa ttaacgattg aatctccatc
     1321 acctgctcca gagaaggcaa cgggattgaa ttcaattcat aggccgaaac agcaacactt
     1381 ccgcttatac acctgaacgc ccgatcgaca acatgcgaat taagtagcat atcaataaca
     1441 tcagggcgaa ccgcagagaa caatccgttt gaatatacaa tattaatatg gttttcaact
     1501 actacgccac cagtttcatc tataaatccc tgaggtaaaa ttgccgctaa aatccttctg
     1561 tcctgttcct tcgatgttgt ccgctgaaca agcacacatt cagattttgt cacaaggaaa
     1621 ccctgttgtg gggttatatc gatataggga acatgatttt ttctgtctgc actaaaacga
     1681 aaaccagctg aagttattga ttccgcccag actaatgggt agctattttt tcctttcgtc
     1741 gtacgcaact gcgacttaaa tcgattccag actaactgac ctgtagagac tgagtatccc
     1801 agatcagcta aacgcgtaga catctgtttt aatacattaa gaaaacaggc atcatcttta
     1861 acgcgtggca aaagccacgt tgctccacct ttttctatat caacattacc tattttctca
     1921 atttttgctt tgttcaaccc cttagggaca agtgaggata cctgggcccg aactttatgt
     1981 tctcccgcct tgaatgctgt caacattgtt tcctggagaa catcgtcaaa gaccccatcc
     2041 ctgtcagtga cgaaatcaag ggcatacgga gtagtttttt cggtcaaaag ggttcgcagc
     2101 gcggtgaaat actgcccacc aagaaaagaa gttggcgtaa gaaatgcaat aactcctgtt
     2161 ttctctttaa ccatgcgaac agctaagtcg gtgaacaatc catatagatt tgcatgacca
     2221 aataacgaac gagaatattt ttctctcgtt ttaatatcga gagtgacacg tccgtaaggc
     2281 ggattcccta ccacaagatc ataaccagaa aatttatctt gctgaagcgc atcagcaaca
     2341 ataattgtat cttcaggcaa tctacgtttt acctttacac atagtggcat tagtacagat
     2401 tcgagcaata ccaaactcat ccaagccgca aatggatcga tctctatccc ctttaaacga
     2461 cgactgattc tcctcaacat ccattcaggg gaagacccct tgtctttttt taacattcga
     2521 atggctactg gtgccaaaaa tgcaccgcca ccacacgcag gatcaataac cgatgcatga
     2581 gaaaaatcaa caccagactt ttcagccagg tccaagagac gcgctaccaa aggtgggggg
     2641 gtatagtaag cccccaattc cgaacggtaa gcggatggca gcattaccgt ataaattgac
     2701 ccaatcagat atccagcgtc ctccgccgga aattgagcaa taagctcacc tgttcgttca
     2761 gcgagctcgc gtgcatcaca tggaatgtct tctatgtaaa cagtgtgcgg tactgccctc
     2821 attttaatgc tgcttttttg cttttttgca agggtatccc aatatgcagc tacgatgctg
     2881 aagcagtatg agcgggcatg catcaacctt gactgcccgg ttttgtaatt gcccgcataa
     2941 cctttggcca tggctttaca agtttgatat ctttccagat tagaaaactc attatctgta
     3001 aaaaaatcaa aatgttgcac attcataatg taaaatccta atgtatcaaa catgcgggcc
     3061 ataacatctc atggtggcgc atagcaaact ggcccgaaga atatcacgca ccaagtcgat
     3121 ctcataaccc gttatgacaa taaatctttg tagctgaact cacttggtga tttgtcaatc
     3181 attcctggcc tccagttcgt cctgaatctc tttatcgagt tcaccgtatt tgttagtcct
     3241 catcactcac gacttgactt ccagaaataa ccttgattat tcggggctat attttgtcac
     3301 cctgcgtatc cgcgctttcg cattacgctc aatctggatt agcttttcta tattttttcg
     3361 cctttcccgc tcctcctggc gcaagagcct tacatcatct gccagtctgg tttctctttt
     3421 cgccacagag agcatccagt caaatggctc cacaactgca ccgcaaattt tacagcggac
     3481 ctgacgctct ttttcgtcaa cccggacaga agcgtgatgg caatatggtc tttccgatgg
     3541 ctcataaaga aaattaacct gattacgagg gttatcctct tttaccggaa ataaaacaat
     3601 attgcttaac tcatcttctg gttttatttc catgctcctc tcctttgatg cgaatgccag
     3661 cggtaattga agcctgatag ctaatttcac tcacagtacc gcctcctgaa aatcaccctg
     3721 atagaaagcc ggtacacgct gcataacttc actcttccgg cactcgcgac agattatgtt
     3781 ctgatgtctg tcgtagcggc gtatttctcc gtccggtaat gaccagataa ggtccggatc
     3841 aaccacagat ggtttcttca gctttgccct cgagagtttt ttgcgggcgt tttgccagtc
     3901 cttacgagcc tgttcagacg ggaataaccc gtagccagag ttgtatacat ccccactggc
     3961 aaccagctct ctggcgagaa cgcttattaa atatcttgtc gccccggttt tagcttccag
     4021 ttgtcgtaac gtctcgcgcc cactctggcg tacgagttca acaacctgcc ccttaatttt
     4081 ttcccgctct tcctgtgtaa atacttttgc cataagcgcc cccggcaatc acttttccga
     4141 cacaatacga ccggaggaat cgacaatctg tcgaacaata tcccggtgct tgttcagctc
     4201 ccgcaacgcg gcgcagacac gctcccactt ctggacatga cttttcgccc gacgcagttc
     4261 gcggtttgcc atatgcagcg atggtaaaac caggtcatcc gcttgcgttt cggtgaacga
     4321 tggcaacgac tgcacaatgt ccgccacagt ttctgtttta atttcttcct gtgttgcagc
     4381 ttcctgtacc ggtaacgcaa cacctgctgg ctgaggaaag actttaccat ccgtttccgc
     4441 tatggatgcg gctttcggct ctgccggtaa atcagcgccc ggtatgcagt accgaaattt
     4501 accgccctga ttcacgcgaa tcagacgccc tttgctgatt gccatggcca gcgatgaatt
     4561 cgcccggcgg gaggtaatcc cgaacatcag tgccagctca tccgccgttt gtgggccatg
     4621 ttgttcaatc gcctcagtca gcatttgcgc tgtcactttc ggtaccggtg acactggttc
     4681 actttcacca gcctgaatca gccaccacat cgaacccttg ttatccgctt caccacggcg
     4741 cttcagtttc cacagttcgt tgaccgcatc ttcacggctg attccaaggc gggacgccac
     4801 cacatgtgaa gaggctcttt tcagtgcttt cagtgcgtca aatacagttt ccattaaaac
     4861 atcctccaac aaaaattact tcacaactcc cggaaagctg acatttgaac gccagctatc
     4921 ccagttaaac gtcacccacc gacaaccgtt catggtcatg cggtccataa tcctctcacc
     4981 aagaagcgtg ctcattgcgg catgattcag gtttgttaac atcccgacac tgcacagtga
     5041 tgctgtccgg cgatcaatta tctggtgcaa taccacctgc tcgtttttcg tctcccgctg
     5101 aatgcctatt tcatccagga ccagcaaatc aaccccgcaa agctcctgta aaaatttttc
     5161 accggatttg ccgttgtcgt agctgtcatg caacacgctc atgacatcag actcggtgac
     5221 gataatcacg ctgcgcccct tcgccatcag ccggttaccc atcgccgctg caaggtgatt
     5281 tttcccggtg ccggttttac cgctgaacac aaaattcgtg cacccggtca tcagttcgtc
     5341 agctatggat ttggcctggc tcagcgcgta tttttgcccg tcgttctgca cctgataatt
     5401 cgcaaacgag catttgctgt gcagaggctg gatgcccgaa cgattcagga ttttttccac
     5461 ccgcaactgg cgattctggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     5521 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     5581 gtgaatacgt tcaagaaccc cggctgccgc aatgtttttc atgccacatc accccctgaa
     5641 tcccggcgga atttcggtat ccggttcaga aatatgattc acacaacgct ggttgttcgt
     5701 gccgcttacc gggagcaacc aggggttttc aaaattccgg tccggcccaa aaaacgtcgt
     5761 cgctcgctga acaaattccg ttcccgcttt cccggtcgcc gccaggtatc tcgcgtaacg
     5821 cctcacacca tccagcatgg tctctggtgg caccccctcg cgcaatctgg ccttccaggc
     5881 actgaatgcg gatttcttcg ggtttgcccc ggcacgcaac gggtattccc gccagacctg
     5941 ttcgaacaca tccggataat ccactcgtcc cacaggctgc ccggtgtttt ccggggctac
     6001 ccgatcggct tcccgctgaa tggcggaatc ggcttcaggc tgctgcagtt ggagtgattg
     6061 ctccggcctt gcggtcatca cctgctgcac agcgcccgaa tcggctttca gcgcatacgc
     6121 tgaatcggct tccggtgtcg agcctgctgg ctgaccagga ttgacggtct gaacatcccc
     6181 tgcctggttc gtggcgtttt ttacgccatg gaccatagtg ttttgatctt ctttatctgt
     6241 atctttatct gtatctgtcg tgactcgtcg tgacatgtgc gtgacatttc gtgacgcgcc
     6301 gtgacaatcg ccattttgtt cccgctttct ttccctctct cgctgcgccc tcttgcgctc
     6361 tgcaggagat tttgcggttt gcgaaatatt gccgttgtcc tctttaagca cctggcgttt
     6421 ttcccatcca gtgattaaat caccatcaag tacccgcccc tgcatcgtct gcaaaattga
     6481 atcaattacc tcttctgtca cgtcgagcgc acttgccaaa tcttctgtcg tgacatcaat
     6541 gtgacctcgc gtgacatttc gtgacgcgct caccaggagg tggatataca ctgccatcac
     6601 tgttgcaatt ggctgccctg acaccctggc aattgttcgc cacttagggt catttggcat
     6661 gtcatgccat aatctgagcc aggcgttagc catactcacc tcttctgata ccgaatcttt
     6721 ttactcacga gttgccggga gcgattcgat atggctattg tcagtcaatg tactgccaca
     6781 gcatttcctg ccgggccacc acggttcatc tgattgaaac cggcgattgc cactgcgaca
     6841 aaatcatcag cgtctctcac cagtcgttca tgcgtctcca ccagttcccg aaaataagct
     6901 gaactgtggc tgcgcattcg agccaccagc ggaggtggca ttgccctttc gatcgcaggt
     6961 aacaacgcct gaattttttc aacagcgtca ggggtgtctt tatccagcca acggaaaatc
     7021 ttttgggtgt tgagataaag cgtgttgtgc ttgctgtcgt tatgcagaac cggaaacgtc
     7081 attcccagtt cgaaataaac cctggttatt tcagctgccg gaactttttc accgtccgga
     7141 tgcgcccagg ctttcatcgc catgcggatg tgttcatgct tgattttcat gaatcaaccc
     7201 ccgccgatgg ttgtgtgtta gcctgaaact cagcaggcaa gccgtcagtt gggttggggt
     7261 aggttttact gtcaatctca tgtggggtca ccacccatcc cgttgcttca caccaacgta
     7321 atattttttc tcctgtaagt tttgcacgcc ccgttataac gtggctgacc atcccttggg
     7381 atgcgccaac aatctcagca aatgaactct gggttatatg aaaatgatgc aaatattctc
     7441 caagattcat aatccgccct atgtgatgtg ttaccaatgc ataattaata gcactgttat
     7501 ttttaaaagt aaatagtgat actatttcaa agtgattagc aatcttatta gaattggatg
     7561 tatgaaaaga aaatcattgt cagacaccga cctcaacgcc gccagaagac tgaaagaaat
     7621 ctggatggca aagaaatctc agttgggatt gactcaggaa cgtgcggcgg aaattatggg
     7681 gttttcaaca caaggggcgg taagtcacta cctaaatggt caaaccccac taaaccttga
     7741 ggctgttcta aagtttgctg gcttactgaa agttccccct gagtcaatca ggccagacat
     7801 ggtagatttg ctgcaaattg caaggatgta ttgtcgagaa ccacagcaag ataacatagt
     7861 cgcattacca gcagacacag aacaaacaga ggatgaacta ccgtttgccg tagagccgat
     7921 ggaacgagat ttaatccata cgttcagagc ttttccgaaa gaagatcaag aacacatgct
     7981 tcaggagatg aaagataaaa aagaatcaat ggatcgtacg gttgcgcggt ggttagctgc
     8041 acaaaaaggc cgtcgcgcct aaatcagagg agatatgtaa aatgagtaca gtcctttccc
     8101 caatggtttc agaatttgag acagttgagc aagaaaatag ttacaacgaa tggctgcggg
     8161 ccaaagtagc agcaagcctc gcggatcccc gcccggcgat tccgcatgat gaggtaatgg
     8221 cggaaatgga acaaattatt gcccaaattg ctgctgcaac tcacagaggt gaataatgtt
     8281 acccgtttta tggctaccat ctgctcgtga tgatttgcgc cagatcgtag cctacatagc
     8341 taaagaaaat cccgcggcag cacgcagaat gaaaattcga attgaaacat ccgttttgcc
     8401 gctcacagag catccttacc tgtatcctcc aagcgagcga gttcttggtc ttcgtgaaat
     8461 tgtaactcat cctaactaca tcatccttta ccgagtaaca gcatcaagca tagaagtagt
     8521 aaatattgtg cattctcgaa gacagtatcc aggcaaaaac agttaattat tcccgccgac
     8581 agaaccacct tagggtggtt ttttcttgcc ccaacaaaaa acaatagcga tgctatttac
     8641 ataaaaaaaa aataatgatg ctattgtttc aggcgtcaac ccaccccgcc ccacagaatg
     8701 cagggcaata cttcgagtta ccagacagtg gtcaggggtt aagtagccag cccgaggcgt
     8761 aagaacatga cggcagggtt caatctttaa ctatgcagca ggttttttgt tccgccaccc
     8821 cggcgttaag gggaaatgag gtcagcatgg atactatcga tcttggcaac aacgaatctc
     8881 tggtgtgcgg cgtgttccct aaccaggatg gaacgtttac cgccatgacg tataccaaaa
     8941 gcaaaacgtt taaaaccgaa tctggcgcgc gttgctggtt agccagaaac actgactgat
     9001 gaggctgacg atggaattta aagatttacc gtctgatgta cagaaaacag cagctcatac
     9061 attgcattct gtgctgcgag aaatcgggaa agatattgca agcgagccag caaaagatct
     9121 ggcccggaaa atcaagaccg ctttcgttga gctttataat gttggcactg actctgaaac
     9181 tgtcgagacc aagaccgtaa gttcaccaat attctcactt gagccaagcg tattatcagg
     9241 tgaaatatgc accgagattt ccagcgaact gcttccggta atacgagaag caattcgccg
     9301 tcgcggtttg gatggaagtt atgatcatga cgttctgcaa gttctcagga caatggtgac
     9361 ttcactgggg atttgatccc tgcgtcctct aagcttttga tatagcggtc ctccaagaag
     9421 cggtaaattt tgtcgaaatc atcttttccc tctggtttat tcagaggatt acaggacccg
     9481 taagtagcgg catacatttc aacc
//
LOCUS       DAJTVE010000148         9422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000148 DAJTVE010000000
VERSION     DAJTVE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            195..1061
                     /gene="folD"
                     /locus_tag="OO439_003528"
     CDS             195..1061
                     /gene="folD"
                     /locus_tag="OO439_003528"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6317807.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1063..1275
                     /gene="ybcJ"
                     /locus_tag="OO439_003529"
     CDS             1063..1275
                     /gene="ybcJ"
                     /locus_tag="OO439_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6317808.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1383..1904
                     /locus_tag="OO439_003530"
                     /pseudo
     CDS             1383..1904
                     /locus_tag="OO439_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1940..3325)
                     /gene="cysS"
                     /locus_tag="OO439_003531"
     CDS             complement(1940..3325)
                     /gene="cysS"
                     /locus_tag="OO439_003531"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6317809.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3499..3993
                     /gene="ppiB"
                     /locus_tag="OO439_003532"
     CDS             3499..3993
                     /gene="ppiB"
                     /locus_tag="OO439_003532"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6317810.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3996..4718
                     /gene="lpxH"
                     /locus_tag="OO439_003533"
     CDS             3996..4718
                     /gene="lpxH"
                     /locus_tag="OO439_003533"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6317811.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4836..5345
                     /gene="purE"
                     /locus_tag="OO439_003534"
     CDS             4836..5345
                     /gene="purE"
                     /locus_tag="OO439_003534"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6317812.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5342..6409
                     /gene="purK"
                     /locus_tag="OO439_003535"
     CDS             5342..6409
                     /gene="purK"
                     /locus_tag="OO439_003535"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6317813.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6546..7439)
                     /locus_tag="OO439_003536"
     CDS             complement(6546..7439)
                     /locus_tag="OO439_003536"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6317814.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7436..8089)
                     /locus_tag="OO439_003537"
     CDS             complement(7436..8089)
                     /locus_tag="OO439_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6317815.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8100..9359)
                     /locus_tag="OO439_003538"
     CDS             complement(8100..9359)
                     /locus_tag="OO439_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6317816.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDRAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2097 a         2672 c         2494 g         2159 t
ORIGIN      
        1 aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct ttcaatctca
       61 gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc
      121 tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac gtaacagatg
      181 gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc
      241 gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcaccaggac
      301 tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca agcaaacgca
      361 aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg
      421 aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc gatggcattc
      481 tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcacc
      541 cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc cagcgcgcgc
      601 cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt tacaacattg
      661 ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgtccaatga
      721 gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc actaaaaatc
      781 tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag ccgggcttta
      841 ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg
      901 aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca
      961 ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc
     1021 tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac attttcttta
     1081 ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc
     1141 ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg cgcggttgaa
     1201 acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta
     1261 caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact
     1321 tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca
     1381 tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta gggttgagtt
     1441 cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg ctgcccgacg
     1501 ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt catcgcgggt
     1561 ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg attggacgcc
     1621 gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc
     1681 acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg tggccgtggt
     1741 cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt gcattgtctt
     1801 gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg gtgtggttgc
     1861 cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat gcgaaaactc
     1921 gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca
     1981 gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc cagtctttcg
     2041 ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt
     2101 ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg cctaatacag
     2161 cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct tctgctttca
     2221 gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg gtgttgaaat
     2281 cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca ggagcaacgg
     2341 ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc gcctgcttca
     2401 ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg aagtaacgca
     2461 cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta cccagcgatt
     2521 tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag ttaacatact
     2581 gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg aacatcaggt
     2641 cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc attgccgaac
     2701 attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc ggttcgccct
     2761 ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg accacgtcaa
     2821 cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca taagtcggat
     2881 cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga cctttggcga
     2941 tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc ggctccatat
     3001 ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca
     3061 ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg tcgtcgatat
     3121 cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag cgcgcaacca
     3181 cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa acggtgattc
     3241 cacacacata catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg
     3301 tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag
     3361 ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat gatccgatcg
     3421 tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag caccaatcaa
     3481 acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa
     3541 acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt
     3601 ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt
     3661 tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga agccaacaac
     3721 ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc gcactctgca
     3781 actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc
     3841 ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga cgtggtagac
     3901 aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac
     3961 gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc
     4021 ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcggggg
     4081 aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg tggattggcg
     4141 acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg gtgtccgatt
     4201 ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg
     4261 cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt tatggccgcc
     4321 gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc
     4381 gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg ttgtttgtgc
     4441 gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc agtaaatcgc
     4501 tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa catcaggtgc
     4561 aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc gccaaccagc
     4621 aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg gtgaaagtca
     4681 cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac tttgctgatt
     4741 tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt gccctctaaa
     4801 gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc
     4861 gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc gccgccgaaa
     4921 tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac cgcacccccg
     4981 ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg attattgcgg
     5041 gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg gtgccggtgc
     5101 tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac tccatcgtac
     5161 aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc gcggcaaacg
     5221 cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac cagcgtctga
     5281 atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg cgaggtacgg
     5341 catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg
     5401 cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg cggcggtgcc
     5461 ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg cattaacccg
     5521 cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta ttgctgaccg
     5581 tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt ggcagttact
     5641 tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg cgattgttaa
     5701 gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca atgaaaccga
     5761 acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta acttctctgg
     5821 tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt atacgctgac
     5881 gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc aggccaacgc
     5941 acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc tgggctatgt
     6001 gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca acgaactggc
     6061 tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca gccagtttga
     6121 gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga ataatccgtc
     6181 ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat tgccgctggt
     6241 gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc atctgaattt
     6301 gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc cgctgctgcc
     6361 gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag caggtgctca
     6421 tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt acccattgta
     6481 ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc cggatgctct
     6541 catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat
     6601 aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac cgccgttacc
     6661 ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg tgtggcatgg
     6721 cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc atcggtgagg
     6781 atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag atctttatca
     6841 atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc gccactgcaa
     6901 atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag aattttacgc
     6961 ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat ctgccagccg
     7021 taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat aaatttctct
     7081 ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac cgtcgtaaca
     7141 ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat catcccctgg
     7201 ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc cagattctgt
     7261 aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg
     7321 gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt ttccgccgtc
     7381 agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag tgttttcatg
     7441 gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct gcccctgacg
     7501 tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg caactcagag
     7561 catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa cgtaaataac
     7621 tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa aactggcgta
     7681 actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc ccggtttcct
     7741 ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc gtaatacgca
     7801 gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc ccttgagcca
     7861 caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat tgcgcgcggc
     7921 gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt aacagttcgc
     7981 cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc caggactggc
     8041 gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt gcgcgttcct
     8101 caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca cgccagcggt
     8161 gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc gataccggta
     8221 ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc caggcacgcg
     8281 ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta aatttccgcc
     8341 atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa gcgcgttacg
     8401 ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat cgctctatcg
     8461 cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc ttgcggagtg
     8521 tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc gaacatattg
     8581 ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc agcatccatc
     8641 gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac gctgaggaaa
     8701 tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg tggggccagc
     8761 gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc gcccatcgta
     8821 atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc cagcgccgcg
     8881 cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac atcttcgccg
     8941 taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta cgcccggttg
     9001 ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat accgcccatt
     9061 ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg ctccagtatt
     9121 gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc cacgcacgcc
     9181 cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg aagcagtgtt
     9241 ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag ccagtgtgga
     9301 cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga tgtaaacatg
     9361 ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca acttattgat
     9421 ag
//
LOCUS       DAJTVE010000149         9420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000149 DAJTVE010000000
VERSION     DAJTVE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..2199
                     /locus_tag="OO439_003539"
                     /pseudo
     CDS             223..2199
                     /locus_tag="OO439_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="OO439_003540"
     CDS             2192..3328
                     /locus_tag="OO439_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6317817.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="OO439_003541"
     CDS             3325..5325
                     /locus_tag="OO439_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6317818.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="OO439_003542"
     CDS             5447..5908
                     /locus_tag="OO439_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6317819.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="OO439_003543"
     CDS             complement(5949..6419)
                     /locus_tag="OO439_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6317820.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO439_003544"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO439_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6317821.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO439_003545"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO439_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6317822.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="OO439_003546"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="OO439_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2291 a         2491 c         2416 g         2222 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
//
LOCUS       DAJTVE010000150         9300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000150 DAJTVE010000000
VERSION     DAJTVE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(252..1505)
                     /locus_tag="OO439_003547"
     CDS             complement(252..1505)
                     /locus_tag="OO439_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6317823.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1612..2505
                     /locus_tag="OO439_003548"
     CDS             1612..2505
                     /locus_tag="OO439_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317824.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2640..3860
                     /gene="mlc"
                     /locus_tag="OO439_003549"
                     /gene_synonym="dgsA"
     CDS             2640..3860
                     /gene="mlc"
                     /locus_tag="OO439_003549"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6317825.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3985..4680
                     /gene="bioD"
                     /locus_tag="OO439_003550"
     CDS             3985..4680
                     /gene="bioD"
                     /locus_tag="OO439_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6317826.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4633..5925)
                     /gene="clcB"
                     /locus_tag="OO439_003551"
     CDS             complement(4633..5925)
                     /gene="clcB"
                     /locus_tag="OO439_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6317827.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6084..6698)
                     /gene="dmsD"
                     /locus_tag="OO439_003552"
     CDS             complement(6084..6698)
                     /gene="dmsD"
                     /locus_tag="OO439_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6317828.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6741..7595)
                     /locus_tag="OO439_003553"
     CDS             complement(6741..7595)
                     /locus_tag="OO439_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6317829.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7597..8214)
                     /gene="dmsB"
                     /locus_tag="OO439_003554"
     CDS             complement(7597..8214)
                     /gene="dmsB"
                     /locus_tag="OO439_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6317830.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8225..>9300)
                     /locus_tag="OO439_003555"
     CDS             complement(8225..>9300)
                     /locus_tag="OO439_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6317831.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2418 a         2364 c         2416 g         2102 t
ORIGIN      
        1 ttagctacca ataatagtag tcttgatcgg gttaactatt ttactactta caaagcggta
       61 gaataactgc gcatcagtaa taaatgacac acagcaaaat gaatccgttt atttgggtac
      121 ttataatcct gatgacgcta gacgcgctgc gggaattggc tggcgcttcg tctattttag
      181 gatggctatt aacgttggtt tgagcaagca ataagtccgg acgtgtagtt accgcagtcc
      241 gggcttattt ttcaggcgtg cagacgacga tgcaaacgcg tcccgaccag cagagcaatg
      301 accagcatca gagcaataaa tgcgccgacg ccgttccagc catagttatg ccagaaaaca
      361 ccacccagcg tcccggcaat actcgacccc agatagtaac tgaacagata cagcgaggag
      421 gcctggcctt tagcgcgttt tgcgcggggg ccaatccagc tgctggcaac tgagtgggct
      481 gcgaagaatc ctgctgagaa gagtaacatt ccggcaaaga tcagccacag cgagctgaat
      541 aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa acaacatcac cggaccgcgc
      601 ccgtagcggg tggtcatggt tccggctttg ggtgagctcc atgtaccggt caaataagcc
      661 agcgataata agccaaccac ggcctgactg acatgccagg gggaaagcat caaccgatag
      721 ccgatgtaat taaacagcgt gacgaacgac cccatcagca aaaagccttc tgcgaacaat
      781 aacggtaatc cccggtcacg ccagtgcaga cgaaagttga taaataacgt cttagggcgc
      841 agcgaagtcg ggcgaaaatg gcgtgattcc ggaaggattt tccagaacat caacgccgag
      901 gctagcgcga aacaaccgat tgccgccaga gcaattcgcc agttgaaaaa gtccgtgaag
      961 acaccactaa ttaagcgtcc gctcatgccg ccaattgagt tgccgctgat atacaacccc
     1021 atcgaaaagg ccacgaaact ggggtggatt tcctcgctaa gataagtcat gccaacagct
     1081 gccacgccac ttaacgaaag cccaatcaag gcgcgcataa tcaaaatgcc gtgccagctg
     1141 gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca gtagcgccgt gaccatcact
     1201 ggtttgcgac caatggcatc ggataggggg ccagtaaaca gcaaaccaat agccaacatc
     1261 gccgtggaaa tggacagtga aatactactg ttcgcggggg ttaagccaaa ctcctgcgaa
     1321 agcaccggaa ggataggctg cacacaatag aggagtgcaa aggttgccag tccggcagag
     1381 aacagcgcca gggtgacgcg cataaattgc ggcgtaccgc gtttaataaa ttgatttggc
     1441 tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc catcaacagt tgtagtacgg
     1501 ctcacttgaa atccttgcta aatatgcctg tggatcaggc ttatacatag gttaggaaaa
     1561 tcgaattgtt ctgtctaata tattaataat ctcaaataag atgttttaaa tatgaatatt
     1621 gaacttcgtc atctgcgtta ctttgttgct gttgcggaag agctgcattt cgggcgcgcc
     1681 gctgcccgcc tgaatatttc gcaaccaccg ctaagtcagc agattcaggc actggagcaa
     1741 caaattggtg cccgactgct ggcacgaact aatcgcagtg tattgctgac ggcagcagga
     1801 aaacagtttc ttgcagatag tcggcaaatc ctgtctatgg tagatgacgc tgccgctcgc
     1861 gctgaaagac tgcatcaggg tgaagcgggg gagttgcgca ttggttttac ttcgtccgct
     1921 ccttttattc gggcggtgtc cgatacgtta tcgctgtttc gccgtgatta tcccgatgtc
     1981 catttacaaa cccgcgaaat gaacactcgt gagcaaatcg ctccgctcat tgaaggaacg
     2041 ctggatatgg gattgctgcg taacacagcg ttaccggaga cgcttgaaca cgcagtcatc
     2101 gtccatgaac cgcttatggc gatgatcccg cacgatcatc ccctggcaaa taacccgaat
     2161 gtaacgctgg ctgaactggc gaaagaaccc ttcgtctttt ttgatccgca cgtcgggaca
     2221 gggctgtatg acgatattct cgggctgatg cgacgttacc atttgacgcc cgtcatcact
     2281 caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct gggtgtttca
     2341 attttgcctg cttcatttaa acgtgttcag ctcaacgaaa tgcgctgggt gccgattgct
     2401 gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga acaaagtcca
     2461 gcttcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa atttcagcga
     2521 aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac gacacgtatt
     2581 gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg gagtatgcgg
     2641 tggttgctga aaaccagctt gggcacattg atcaaataaa gcagaccaac gcgggcgcgg
     2701 tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc cgtctggcgc
     2761 agctggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca cacctggtgc
     2821 aagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg ctggtggttg
     2881 aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt ttccttgctc
     2941 tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg ttaaaagatg
     3001 acttgccatt gctggatcgt atcatttccc atatcgatca gttttttatc cgccaccaga
     3061 aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt gatacggaaa
     3121 atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg ctcggcgagg
     3181 cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc gcatggacga
     3241 tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag gtggttatcg
     3301 atcacaacgt gggggcgggc gtcattaccg atggtcatct gctacacgca ggcagcagta
     3361 gtctcgtgga aataggccac acacaggtcg acccatatgg taaacgctgt tattgcggga
     3421 accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg gcacagctgc
     3481 gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg gactcattgt
     3541 gtcaggcggc attgcgcggc gatctactgg caaaagacat cattaccggg gtgggcgcgc
     3601 atgtcgggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa atactgattg
     3661 gctcaccgtt aagtaaagcg gcagatatcc tcttccctgt catctcggac agcatacgtc
     3721 agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag ttttctaacc
     3781 agggcacgat ggcaggcgct gcgctggtaa aagacgcgat gtataacggt tctttgttga
     3841 ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg ctatctcaat
     3901 ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctag tttatatttg
     3961 tgaagcataa cggtggagtt agtgatgctg gagcgtttct ttattaccgg tacagacact
     4021 tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc ccagggaaaa
     4081 acagttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga agggctgcgt
     4141 aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg aactgcctta tgaagcagtt
     4201 aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc aatcaattac
     4261 accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt cgtggtagaa
     4321 gggactggcg gctggcgcag tctgatgaat gatttgcgtc cactctctga atgggtggtg
     4381 caggaacagt tgccggtgtt gatggttgtc ggtattcagg aaggttgcat taaccatgca
     4441 ctgctaacag ctcaggcgat tgccaacgac gggctgccgc tcattggctg ggtggctaac
     4501 cgaatcaacc caggactggc gcattatgcg gaagtcattg atgtgctggg taaaaaactt
     4561 ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg agaactgggg
     4621 caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt cacggtgtaa
     4681 cgtccgcgaa attaccgacg caatgacgca ggcaatcaat aaaccgggga gtagctgata
     4741 ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg tggttgccgc
     4801 caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc catcagggaa
     4861 ccataatccc aggctacgac catacaacat gccaatggca agaccgataa atagcgtcgg
     4921 tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc agcacggcac acagtttaca
     4981 aaggaagatc ccggcaatga tcattaacag tggtggggcg gttaaaaagg attgtacggt
     5041 gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca cgatcaaccc
     5101 gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac gatgacaggc
     5161 gttcattaac gttaacaaca gtggtccgca cagacctgcc agcacacctg tactgataat
     5221 cagcgcatag tcactagcct gaaccgttac tgagagttgt acgctgtaga gtaacgcgtc
     5281 gctatgatta ataagattgc taaccagcaa cgccacgacg gcggaaataa tcaccgggcc
     5341 gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag
     5401 cggggcgcga taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca
     5461 ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gaaagggcag ctaaaagaat
     5521 catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga
     5581 tttaaccagg cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc
     5641 ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc
     5701 cgccagtccg ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc
     5761 attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg
     5821 acgaaacccg gcaacggcaa aggccgcgag aataccgacg actgttgcga taagcagacg
     5881 gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca
     5941 atgaaacacg ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt
     6001 taagagataa aaaaacgtga cattgtaact tattattgcc gaatcgcgcg taatcgtcac
     6061 catccggcaa tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac
     6121 agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa
     6181 gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc
     6241 catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc
     6301 aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct
     6361 ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga
     6421 tccagccaga ccgatcccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc
     6481 tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct
     6541 aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc
     6601 agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa cacgcgcgcc
     6661 gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg
     6721 ggttgccccg caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca
     6781 taaaagagca cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt
     6841 ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg
     6901 cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct
     6961 tgttgcacgg atgagtgaat agacgccaga gacaaacctt gcagcacaat caccccggca
     7021 caagcgatca gcgcgagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg
     7081 gctgcccgca gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa
     7141 gccagcgtag tgtaaccgtt atgccaggtt ggcacagtgt cgatttgata cacgcaggtc
     7201 atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac
     7261 gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat
     7321 atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta
     7381 aacgcccgca gtggcgatcc caggtgcatg acagaggcca taaacccgat gcccatcaac
     7441 agccagagga aaaacatgcc acggacaata cgctgacggt ctgcgtcatt tttggcggcg
     7501 aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag
     7561 ataaccagtg gccactcatg ccatccattt cccattttac acttcctccg gattagccag
     7621 ataaccagtg gtatcgctgg tcgggcggct gttggcgtta ggtttgataa cgatattggg
     7681 ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc agagtgccgt gcttctggcg
     7741 cagctcttca attggaccga actcaagtgc acgcagcggg caggattcga cacatatggg
     7801 ttgtttcccc tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc
     7861 agcattgtac tgtggcgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca
     7921 aacatcttca tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca
     7981 gacttttgta catgcggggt catcgcaatg gttacaggag atggagagat aataagcgaa
     8041 cacgttctgg tgccagacgc cattatcttc ctgccagtcg ccgccagcat attcataaat
     8101 acggcggaaa ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt
     8161 tttacagcca gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcacgggcta
     8221 ctccttaaac cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgccagcg
     8281 gtgaagggcg gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa
     8341 acatatccgc tttaagccaa gcaccttgcc cgatggcggt aacgccaggc agaatacgcg
     8401 gagtcacttt tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc
     8461 catgacggat accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg
     8521 cctgctgtaa cacatcaata ttgccgtagc tggagtgggt acgcgctttg taatggaagc
     8581 ccgttaactg cagtggatag gttttccgca ggggatcgtc ccagccatca aaacctgggg
     8641 tatacgcagg aaggggatga ataacttcat cttttttcaa ttcccaggta tccgcaatct
     8701 tcgccagtcg ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct
     8761 gtggatcttc acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa
     8821 agatcccggt cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt
     8881 tcgcatggag atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa
     8941 cgtctggtcc taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa
     9001 attttgctga ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat
     9061 gagagataag gtcttcttgc tctgttggca tcaggtcggg caacaggata tcgcaatact
     9121 tagccgaggc cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct
     9181 gaagcacctc atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt
     9241 agcaccataa aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg
//
LOCUS       DAJTVE010000151         9178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000151 DAJTVE010000000
VERSION     DAJTVE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..154)
                     /locus_tag="OO439_003556"
     CDS             complement(<1..154)
                     /locus_tag="OO439_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6317832.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLA"
     gene            complement(154..1080)
                     /gene="glsB"
                     /locus_tag="OO439_003557"
     CDS             complement(154..1080)
                     /gene="glsB"
                     /locus_tag="OO439_003557"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6317833.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1144..2150)
                     /locus_tag="OO439_003558"
                     /pseudo
     CDS             complement(1144..2150)
                     /locus_tag="OO439_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            2251..3132
                     /locus_tag="OO439_003559"
     CDS             2251..3132
                     /locus_tag="OO439_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6317834.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3209..4324
                     /gene="yneK"
                     /locus_tag="OO439_003560"
     CDS             3209..4324
                     /gene="yneK"
                     /locus_tag="OO439_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6317835.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4474..5664
                     /gene="ydeA"
                     /locus_tag="OO439_003561"
     CDS             4474..5664
                     /gene="ydeA"
                     /locus_tag="OO439_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6317836.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5689..6354)
                     /gene="marC"
                     /locus_tag="OO439_003562"
     CDS             complement(5689..6354)
                     /gene="marC"
                     /locus_tag="OO439_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6317837.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6566..7000
                     /gene="marR"
                     /locus_tag="OO439_003563"
     CDS             6566..7000
                     /gene="marR"
                     /locus_tag="OO439_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6317838.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7020..7403
                     /gene="marA"
                     /locus_tag="OO439_003564"
     CDS             7020..7403
                     /gene="marA"
                     /locus_tag="OO439_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6317839.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7435..7653
                     /gene="marB"
                     /locus_tag="OO439_003565"
     CDS             7435..7653
                     /gene="marB"
                     /locus_tag="OO439_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6317840.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKPDALGVPYYNQHAM"
     gene            complement(7710..9149)
                     /locus_tag="OO439_003566"
     CDS             complement(7710..9149)
                     /locus_tag="OO439_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6317841.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2377 a         2329 c         2079 g         2393 t
ORIGIN      
        1 gagcaagacg tttagcgaca aaatccgccg catgttgttc gatgactggc gcgcctttct
       61 ccaggcaata ctgacgctct ttcatgccca gacgaaagcg agagcgaaat tttgaacggg
      121 ataaacgcgc gaagagtgga tcgagagact gcattaataa accgaacgcc ctaactgttt
      181 cgtcaattgt tcaaggacgg cgatacccgc aagcgagtta cctgcatcat ccagttccgg
      241 actccagact gcgatggcca tttcatgcgg aacaatcgcc acaataccgc caccaacgcc
      301 agatttcgcc ggtagtccca cccgccaggc aaactccccc gcgttctggt acataccact
      361 ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc gtcactacag gttcatcaat
      421 atgaatagct ttcccctgat tagccagaaa gacaaacgtc cgagccagct ctacacagct
      481 cattttcaga gcgcagtaat gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa
      541 attgccaaac gacttcatca gccaggcgat agccgcattt cgcgcggaat gttcaaattc
      601 ggaacgcgct accaccgtat cgtatgaaat atcagacaca ccgcttaagc cgcgcacgac
      661 ttccagcata cgttgccgtg gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag
      721 cgcaccagca ttaataaacg gattacgcgg aataccctgt tccatttcca gttgcactaa
      781 agaattgaac ggtgatccag acggatcttt gccgacgcgt tgccagattt cctcttcgga
      841 gtaatgacgc atggcgacaa cgagactcag cactttggaa atagactgaa tggaaaaacg
      901 ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg gtacagatag caatgcccaa
      961 tcgggaaccg tctactgtag ccagcgccgg aatataatcc gcgactttac cctgaccaat
     1021 gagcggccgc acttgccgca agatgttttc taaaattgca ttatccatgg cgactgccac
     1081 tttctactcc tggaccgcag gtctgaaaag acctgcgagt atatcagagc tgaatatgtc
     1141 gcgtcagatc cggtctttcc acaccgtctg gatattacag aattcgtgta agccgaaatg
     1201 ggaaagctca cgaccaaagc cactcttttt cacgccacca aaggccactc gcgcgtcgct
     1261 ggcacaataa ccattgataa acaccccacc gcattccaga cgtgccgcca tctgtctggc
     1321 ctgtgtttcg tcggtggtaa aaatggtcgc tgaaaggccg aactcactat cattagccag
     1381 ttccagcgca tgttctgcat ctttcgcaac ggtgattgcc gcaacggggc caaacatttc
     1441 ttcccgaaac gcggtcattt ctggggtaac attcgccaga accgttggcg gatagtagct
     1501 ccagcacgaa cacttccggt caccgtgaca gcagcaatgc gcgagtcttt aatcatctga
     1561 ctgacaccgt cgttgtcggc attcagccag ccatatacgc cttgcgggat tcccgcatct
     1621 ttaaacacct gggcaatgag ctgagcacat cccatcacat tcggcgcatg tttaagtaag
     1681 tagccgttac ctgcaagaat gatgggaaca gcgccacgca tcacctgcca taacggaaaa
     1741 ttccacggca taatcgccag aatcgtcccc aacggtcgat actcaataac cgcctgctga
     1801 ttttccacca gtgtaggttc cgccttcagc attgccggac catgttctgc ataccagtca
     1861 cacaaattcg ccgatttcgc cacttcagcg cgcgcctggt tgattggttt gcccatttcg
     1921 cgggtgatca tttgcgccat ttcttcgcta cgggcgcgca gagccttacc gatatcacgc
     1981 attttttcag cacgataatc tatatttgtc tcgcgccagt cgcgaaagcc tgctgccgcc
     2041 agctcaagtg cgttttcgat atcgttagcg ccagcccacg gcagcacaga aagttgttca
     2101 cccgtggcag gatttattga aattgcatga gttgccggag taatggtcat cggggtatct
     2161 cctttatgag tcatggtatg aagatacgca gatttactct tgctttaaaa tgaataatat
     2221 taagccactt attcacgaat cgagaatgtt atggatctga cccaactgga gatgttcaac
     2281 gcggttgccg aagctggcag cattacccag gctgcagcaa aagtgcatcg cgtaccgtcg
     2341 aatctcacta cccgtctacg ccagctggaa acagaactgg gggtagatct gtttattcgc
     2401 gagaatcagc gtttacgtct ctctcctgcc gggcataact ttttacgcta tagccaacaa
     2461 attctcgcgt tagtggatga agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta
     2521 ttttctcttg gttcgctgga aagcaccgca gcagtgcgca ttccggccac gctggcggaa
     2581 ttcaaccatc gttatcccaa aattcagttt tcactttcca ccggcccttc cggcactatg
     2641 ctggaaggag tgctggaggg aaaactgaat gcggcattta ttgatgggcc cattaaccat
     2701 actgccattg acgggatacc ggtataccgc gaggagctga tgatcgtcac gccacaaggt
     2761 catgcgccag taatccgcgc cagtcaggtt aacggcagta acatttatgc cttccgcacc
     2821 aattgttcgt atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc cgctccggga
     2881 actattcacg agatggagtc gtatcacggg atgttggcct gtgtggtcgc aggagcaggc
     2941 attgcgctta ttccacgctc tatgctggaa agtatgccgg ggcatcacca ggttgaagcg
     3001 tggccgttag ctgagcaatg gcgttggtta acaacctggc tggtctggcg tcgcggtgcg
     3061 aaaacgcgtc cgcttgaggc atttattcaa ctactggatg tgtctgactc ggcaaaacag
     3121 agttaccaat gaactatttt gataattctt gcggctaata tgctctatat agtgatgttc
     3181 cgatgactta tgactatatg gggcaaatat ggttacgcca gtaagcatca gcaattacat
     3241 atctcttcct gatgattttc ccgttcgtaa tattgcacca caggtaaaag aagttttaaa
     3301 ggactttatt gatgcgctaa gtacaataat atgtgatgaa gagtggcgca cgtctttaaa
     3361 catcaactca gccacaaaaa agatatttaa taaccttgat aacttatcat atattcagag
     3421 gacttctttc cggggtaacg acacgctata caatgaaaag gttcagttta aactcactta
     3481 tcccgtcaag aatggaagac acaaagaaaa tattgaattt caggtagtaa taaatttaag
     3541 ccctatttat ttagataatt ttcgccatga tggagaaatt aatatttttt gcgctcccaa
     3601 cccaaagcct gtcactatgg ggcgcgtatt tcagaccggc gtcgagcgtg tgctctttct
     3661 gtttctgaat gattttattg aacaatttcc aatgttcaac cctggcgttc cgatcaaaat
     3721 agcgcataca ccacatattg aacccctgcc tccggatcac cataccgctg cagattactt
     3781 acgccagttt gatttgcttg tcctgaattt tatctctcgc ggtaattttg tcatactccc
     3841 ccgattatgg aataactctg aggttcacag atggttcgtc aataaagatc ctaacttgat
     3901 caccgccatt ctcgacataa cggacagcga attaaaagag gatttattgc aaagcctgat
     3961 ggattcatta ggttctaaca aacatgtact acccgaagtc tgcatctgct ttttattcct
     4021 tttagcagaa caagaatctc ctcattttca ggacttgttt ttatttttcg ccaatatgtt
     4081 attgcactat caccaattta tgaatcccaa tgaaagtgat ttgaatgacg tgttgatgcc
     4141 agcatcatta agtgatgata aaattatcaa gcatatggcg cgcaggaccc tcgaactgtt
     4201 tgtaaaaaat gaaacaccgc caaaagttac tcacgaagac ctggtgaaaa acaggcctcg
     4261 ctcccctgtc agaccaccta tacccgcaac ctccaaaacg ccagacctcc ctgaacgtca
     4321 ttaaaccgtg atgttaccga ctctctgacg cggaacagaa tcagcgtcag agaaacggaa
     4381 aacgcgatcc agatcacaaa tacattgtat tcacatcatt aaccgtttta agatcatttc
     4441 atcacttttt cgcaactcac ccgataatct gttatgacaa caaacactgt ttcccgcaaa
     4501 gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct tcatcttcaa caccaccgaa
     4561 tttgtccctg ttggcctgct ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag
     4621 gtcggcatca tgttgaccat ttacgcatgg gtagtagcgc taatgtcatt gccttttatg
     4681 ttaatgacca gtcaggttga acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt
     4741 gccagccacg tactgtcgtt tttatcgtgg agctttaccg ttctggtgat cagtcgcatt
     4801 ggtgtggctt ttgcacatgc gattttctgg tcgattacgg cgtctctggc gatccgtatg
     4861 gctccggccg ggaagcgggc acaggccttg agtttaattg ccaccggtac agcactggcg
     4921 atggtcttag gtttacctct cgggcgcatt gtggggcagt atttcggttg gcgaatgacc
     4981 ttcttcgcga ttggtattgg ggcgcttatc acccttttgt gcctgattaa gttacttccc
     5041 ttactgccca gtgagcattc cggttcattg aaaagcctcc cgctattgtt ccgccgcccg
     5101 gcattgatga gcatttattt gttaactgtg gtggttgtca ccgcccatta cacggcatac
     5161 agctatatcg agccttttgt gcaaaacatt gcgggattca gcgccaactt tgccacggca
     5221 ttactgttat tactcggtgg tgcgggcatt attggcagcg tgattttcgg taaactgggt
     5281 aatcagtatg cgtctgcgtt ggtgagtacg gcgattgcgc tgttgctggt gtgtctggca
     5341 ctgctgctac ctgcggcgaa cagtgaaata cacctcgggg tgctgagtat tttctggggg
     5401 atcgcgatga tgatcatcgg gcttggtatg caggttaaag tgctggcgct ggcaccagat
     5461 gctaccgacg tcgcgatggc gctattctcc ggcatattta atattggaat cggggcgggt
     5521 gcgttggtag gtaatcaggt gagtttgcac tggtcaatgt cgatgattgg ttatgtgggc
     5581 gcggtgcctg cttttgccgc gttaatatgg tcaatcatta tatttcgccg ctggccagtg
     5641 acactcgaag aacagacgca atagttgaaa ggcccattcg ggcctttttt aatggtacgt
     5701 tttaatgatt tccaggatgc cgttaataat aaactgcaca cccatacata ccagcaggaa
     5761 tcccatcaga cgggagatcg cttcaatgcc acccttgccc accagccgca taattgcgcc
     5821 ggagctgcgt aggcttcccc acaaaataac cgccaccagg aaaaagatca gcggcggcgc
     5881 aaccatcagt acccaatcag cgaaggttga actctgacgc actgtggacg ccgagctaat
     5941 aatcatcgct atggttcccg gaccagcggt acttggcatt gctagcggca caaaggcaat
     6001 attggcactg ggttcatctt ccagctcttc cgacttgctt ttcgcctccg gtgaatcaat
     6061 cgctttctgt tgcggaaaga gcatccgaaa accgataaac gcgacgatta agccgcctgc
     6121 aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc accagttgcc cggcgtaata
     6181 cgccaccatc atgatggcaa atacgtacac cgaggccatc aacgactgac gattacgttc
     6241 ggcactgttc atgttgcctg ccaggccaag aaatagcgcg acagttgtta atgggttagc
     6301 taacggcagc aacaccacca gccccaggcc aattgcttta aacaaatcta acattggtgg
     6361 ttgttatcct gtgtatctgg gttatcagcg aaaagtataa ggggtaaaca aggataaagt
     6421 gtcactcttt agctaacctt gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg
     6481 tggcatcggt caattcattc atttgactta tacttgcctg ggcaatatta tcctctgcaa
     6541 ctaattactt gccagggcaa ctaatgtgaa aagtaccagc gatctgttca atgaaattat
     6601 tccattgggt cgcttaatcc atatggttaa tcagaagaaa gatcgtctgc ttaacgagta
     6661 tctgtctccg ctggatatta ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc
     6721 ggcgtgtatt actccggttg aactgaaaaa agtgttgtcg gtcgacctgg gagcactgac
     6781 ccgtatgctg gatcgcctgg tctgtaaagg ctgggttgaa aggttgccga acccgaatga
     6841 taagcgcggc gtactggtaa aacttaccac cagcggcgcg gcaatatgcg aacaatgcca
     6901 tcaattagtt ggccaggacc tgcatcaaga attaacaaaa aacctgacgg cggacgaagt
     6961 tgcaacactt gagcatttgc ttaagaaagt cctgccgtaa acaaaaaaga ggtatgacga
     7021 tgtccagacg caatactgac gctattacca ttcatagcat tttggactgg atcgaggaca
     7081 acctggaatc gccactgtca ctggagaaag tgtcagagcg ttcgggttac tccaaatggc
     7141 acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt aggccaatac atccgcagcc
     7201 gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa cgagccgata ctctatctgg
     7261 cagaacgata tggcttcgag tcgcaacaaa ctctgacccg aaccttcaaa aattactttg
     7321 atgttccgcc gcataaatac cggatgacca atatgcaggg cgaatcgcgc tttttacatc
     7381 cattaaatca ttacaatagc tagttgataa cgtgacaacg tcactgaggc aatcatgaaa
     7441 ccactttcat ccgcaatagc agctgcgctt attctctttt ccgcgcaggg cgttgcggaa
     7501 caaaccacgc agccagttgt tacttcttgt gccaatgtcg tggttgttcc cccatcgcag
     7561 gaacaaccac cgtttgattt aaatcacatg ggtactggca gtgataagcc ggatgcgctc
     7621 ggcgtgccct attataatca acacgctatg tagttattgt caggctctga tcacagagcc
     7681 tgacatctcc gtaatatctt cctcaagtct caaatatttt cgccattgtt ggcgatgaga
     7741 tcccgatacc agtaaaacga cgctttacgt atccgttcca gcgttccctt cccttcgttg
     7801 tctttatcga cataaatcat tccgtagcgc tttttcattt cccctgttcc ggcagaaacc
     7861 aggtcaatgc agccccacgg ggtgtagcca attaagtcaa caccatcttc aactacggct
     7921 tttttcattt cccgaatatg ggaagcaaag taatcaatgc gatagtgatc gttcaccgtg
     7981 ccgtcagctt gtctctggtc aaccgcacca aatccatttt cgacaataaa cagcggcaac
     8041 tggaaatgat cccagaacca gttgagtgaa taacgtagcc ctgccggatc aatttgccat
     8101 ccccattcgg atgtatcgat ataagggttg ctgaccaaat ctcgtggttc aacataatcc
     8161 agttgcgggt tatcgggaga aaattgtgtc gtaaaagaca tgtagtagct aaaaccgata
     8221 aagtcgacac atccactggc aagaatcgcg ttatcttctg gtgtgatatc gaggttgaat
     8281 cctttcctgg caaagtaatt cagcatatgt tgcggataat atccacgagc atgaacatca
     8341 gtaaaccagt aacgacgatg catcgctttc gtggccatca tcatatcgtt gggtgcacac
     8401 gtcagaggat agatggggca catggcgatc atacagccaa tgttaaattc aggattgatc
     8461 gattttccag tctgtaccgc cagggcactg gcaactaact cgtaatgtac cgcctggtac
     8521 attatttgtt cgcgctcatt gatatcttcc tctggcgaat acaagatacc ggaattagta
     8581 aatggacaca ggctttcgct gaaattcacc tgattattga tttcgttaaa cgtcatccag
     8641 tactttactt tttctttata gcgcgtgaag accgttgatg cgaagcggat aaaaaagtcg
     8701 atcagcttac ggtttcgcca gccaccatat tttgtcacca gatgataagg catctcaaaa
     8761 tgcgaaagcg tcaccacagg ttccattcct tgcttcaggc attcatcgaa cagatcatca
     8821 taaaattgta aaccctcttc attcggctcc tgttcgtcac cttgcggaaa gattcgtgtc
     8881 caggcaatgg aagttcgaaa gcatttgaat cccatctcgg caaataactg aatatctgtt
     8941 ttatagcgat gataaaaatc aattgcttca tgattgggat aattaagccc gtcgataacg
     9001 ccttctgtca cttcacgcgg caccccgtga gcgccagcag tcatcacatc agcgatactg
     9061 atgccttttc ctccttcatt ccagccacct tccaactgat gcgcggctac cgcgccaccc
     9121 cataaaaaac cttttttaaa tcctgacata cacaactcct taactaaaat agattacc
//
LOCUS       DAJTVE010000152         8858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000152 DAJTVE010000000
VERSION     DAJTVE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..98)
                     /locus_tag="OO439_003567"
     CDS             complement(<1..98)
                     /locus_tag="OO439_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6317842.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
     gene            complement(123..1223)
                     /locus_tag="OO439_003568"
     CDS             complement(123..1223)
                     /locus_tag="OO439_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6317843.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1611..2358)
                     /gene="cutC"
                     /locus_tag="OO439_003569"
                     /pseudo
     CDS             complement(1611..2358)
                     /gene="cutC"
                     /locus_tag="OO439_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
     gene            complement(2372..2938)
                     /locus_tag="OO439_003570"
     CDS             complement(2372..2938)
                     /locus_tag="OO439_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6317844.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="OO439_003571"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="OO439_003571"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6317845.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5067..5429
                     /locus_tag="OO439_003572"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="OO439_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="OO439_003573"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="OO439_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..7431)
                     /gene="flhB"
                     /locus_tag="OO439_003574"
                     /pseudo
     CDS             complement(6496..7431)
                     /gene="flhB"
                     /locus_tag="OO439_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            7584..8603
                     /locus_tag="OO439_003575"
     CDS             7584..8603
                     /locus_tag="OO439_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6317846.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         2334 a         2265 c         2170 g         2089 t
ORIGIN      
        1 tcttctgccc atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata
       61 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
      121 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
      181 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
      241 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
      301 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
      361 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
      421 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
      481 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
      541 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
      601 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
      661 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
      721 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
      781 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
      841 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
      901 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
      961 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
     1021 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
     1081 gatttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
     1141 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
     1201 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat
     1261 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
     1321 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
     1381 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
     1441 atataaaaaa tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg
     1501 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
     1561 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
     1621 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
     1681 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
     1741 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
     1801 tggtgcagat tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
     1861 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
     1921 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
     1981 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
     2041 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
     2101 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
     2161 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
     2221 acggatttca gtacccccct acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtcat tgaaaatgta
     7441 atgaacgcat cccacacaat taaacacgga gcagtatgat ggttaaaagg gtgaaactgg
     7501 caggtgtgga gcctcgcaga tttccggcat catagtgccc aatacgggat gcataaagct
     7561 ccctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat atcgcaaaac
     7621 atgtcattca gattcacttc atcaatgagc acacaggtga agtggttgat aaacagttgc
     7681 gtagacagga ttttctgacg ttcttcggca accgtgagcc atgcctgatt ggtatggagg
     7741 cctgtggagg ttctcagcac tgggcacggg aactgacaaa acttggtcat aaagtccggt
     7801 tgttgcaggc ccgcttcgtt aaggcattcg tcatgggcaa taagaatgat gtgatggatg
     7861 cccgggctat ctggatggcg gttcagcagc cgggtaaaga aatcgccgta aaaacagaag
     7921 aacagcagtc ggtactggtt ctgcaccgta cccgcatgca actggtgaag ttccggaccg
     7981 cacaaattaa tgccctgcac gggacgttac tggagtttgg tgaaaccatc cacaaaggcc
     8041 gggcagcgat ggagcgggag ttccccgaag cactggaacg gatgaaagag agactgccac
     8101 cgtatctcat tatggttctg gaaaaccagt acaaccgact gaatgagctg gactcactga
     8161 tagaggatat tgaaaaacag cttaccagcg tggcgaggca gaatgaaacc tgtaagcggt
     8221 tgctggatat tcctggcgtt ggaccactta ttgcgacggc agcggtggcc accatggggg
     8281 aagcatcagc gtttaaatcg gggcgagagt tcgccgcata tgttggtctg gttccaaaac
     8341 aaactggctc cggagggaaa gtacgtctgc tggggataag caaacgtggt gacacttatc
     8401 tcaggacatt atttatccac ggtgcaagag cggtggcatt agtagctaaa gagcctggcc
     8461 cgtggataac cgaactgaaa aaacgtcgtc cagccagtgt ggcaatcgtc gccatggcaa
     8521 acaagctggc acgaacagta tgggcgataa ccgcccatga ccgtaagtat gacaggaacc
     8581 acgtcagtat cagaccatat taatcgctga taccattaaa caatgaactc ttaacaaaag
     8641 ggtgaatgct gaaaggttgc tatggcggcc agagtgatga caaagacagg taagaccgtg
     8701 actcactaaa cctgaacagt attttgggct tgaagtccgc cgtgaaaata aggggtgagt
     8761 cggcgaatta cataggggct cgcagcgtta cggctgcaat aaagccggat ataaagctgc
     8821 aacctacccg tcatgtcaaa acaatggatg ccttgcaa
//
LOCUS       DAJTVE010000153         8848 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000153 DAJTVE010000000
VERSION     DAJTVE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            374..1249
                     /locus_tag="OO439_003576"
     CDS             374..1249
                     /locus_tag="OO439_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6317847.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1257..2033
                     /locus_tag="OO439_003577"
     CDS             1257..2033
                     /locus_tag="OO439_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6317848.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2202..4463
                     /locus_tag="OO439_003578"
     CDS             2202..4463
                     /locus_tag="OO439_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6317849.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4532..5707
                     /gene="senB"
                     /locus_tag="OO439_003579"
     CDS             4532..5707
                     /gene="senB"
                     /locus_tag="OO439_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6317850.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6233..6694)
                     /locus_tag="OO439_003580"
                     /pseudo
     CDS             complement(6233..6694)
                     /locus_tag="OO439_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6732..8270)
                     /locus_tag="OO439_003581"
     CDS             complement(6732..8270)
                     /locus_tag="OO439_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6317851.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(8320..8667)
                     /gene="tnpB"
                     /locus_tag="OO439_003582"
     CDS             complement(8320..8667)
                     /gene="tnpB"
                     /locus_tag="OO439_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6317852.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT         2308 a         2197 c         2148 g         2195 t
ORIGIN      
        1 taacgtacgt tttcgttcca ttggccctca ccccccgata acgacccagg tagcgcgccg
       61 tattcccccc gccggccgtt ttcttcgaca tgtacatcag gcgcggaact catcggggcg
      121 cgggaatgaa atcccattca gactccggat agattcaagc aagcccactt atcgtcaaaa
      181 tcggtgttgc aaaaacggga gtgaccatag atccgttttc caagcggacc ccataatatc
      241 ggctggtaaa catcactcgc tagtgcattc tttttgttgg taatgataat catttacatt
      301 cattctttga tgttttatca taattaatgt tttgtcaata aatatatgga aacatattgt
      361 ttcagatggg aatatgagaa agttcatcct tatttctatg ataacgttag ctagtgttag
      421 tctgggagcc tgcagacaga atgtcaccat aaagcaggat gcgccagggc agaaagcttt
      481 cctgactgac gggcagattt acgatttgca cagtggcaaa attatttcta gcagcgagct
      541 tttggcggat cttgctaccg ctcgacatct tattattggt gaaaaacacg ataatgccga
      601 gcatcatcag attgagcttt ggcttattca aaatttgctc atccagcgac cacaggggag
      661 tgtgttgctt gagatgttga cgtcagagca acagccccga gttaaccaag ttaaatgttg
      721 gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag gaactactta actggcaaaa
      781 agggtggtcc tgggagatgt atggcgacat cgtcatgcag ctattacgtg ggccttaccc
      841 gctgctcaat gccaacattg gccgggagca gatgttggca ctgtacaaaa aaaacgaatt
      901 tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa gctctgcgtg aaaccatcat
      961 atctatgcat gaaggcaacc ttgaaagcca gcaactgacg tcaatgctct ctattcagca
     1021 gcagcgggat cgctacatgg cccgccagct tctgagtgcc ccggtaccgt cgttgttgat
     1081 tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg ttacacatgg aagatcttgc
     1141 caccggcacc catccagtgg tgttgatgct tgcagaaaaa ggaatgaaca tcacagtaga
     1201 tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca aaaaggtaaa ccatggatga
     1261 tgaatattct ccacttctca cagtcggtaa aatggagcag ctggtttatc tgttctctac
     1321 tgcttcatgg actgattttc cttgcgttta tctggcgttt ttccgaagtt cagcccgcta
     1381 tgtctcctgc accggcgatc atgctgcaat gggcagaaat tatagaggct ccttcttctc
     1441 cactttcgct tccggtggga atcgctcaac aggagtccgc agtgacagaa gaaaagcaac
     1501 aaactgagga cagacaacaa cgtcctgtca ctgaagacag tgatgcaacc attgaaataa
     1561 ctaggaagaa aaagtcatcc gatggagaaa agaaaaagac acgtccacct cgtaagataa
     1621 aggctcaaac cagcgacagc aaccccacag ccgtttccag taatgctgca cctcaggctc
     1681 tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga ctcaacaaag cgagataatt
     1741 ctgaggcatc ttgggaaagc agagtaaaag gccacctaaa ccgttataaa cgttaccctg
     1801 gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt gacttttacc gtaaacacgg
     1861 aaggcactat tgtcagtagt ttcttggaaa tctcttcagg aaccctttct cttgaccgcg
     1921 aagctattgc tgttttagaa cgcgctcaac cacttcccaa accaccgcct gaaatattgg
     1981 agggaggatt atttaaagta aaaatgccga ttacctttaa gttgaaggag taaagggata
     2041 attggccgtt caagctcatt taactgaaat ttaattttca tttttatgct gaacatttta
     2101 tcaaactttt ccactgtcga aaaaatcaaa aagacagtag aacataatta ataaacaaat
     2161 agttatactt atctttatta atattatacc caaggaaatt tatgaacgtt ataaaactgg
     2221 ctatcggctc aggcatatta ttgctcagct gcggtgctta ctcacaatcc atcagtgaaa
     2281 aaactaattc cgacaaaaaa ggagcggcag aattcagtcc gctcagcgtt tctgtcggga
     2341 agacgaccag tgagcaggaa gctctcgaga aaacaggcgc gaccagttcc cggacaacgg
     2401 acaaaaacct gcaatcactt gacgcaacag tgcgtagtat gcctggtact tatactcaaa
     2461 tagatcctgg tcagggagca atcagtgtga atattcgagg catgagcgga tttggtcgtg
     2521 taaacactat ggtcgatggt attacccaga gtttttacgg aacctctacc tccggaacaa
     2581 cgacgcatgg ttcaactaac aatatggctg gcgtacttat agatcctaac ttactggtag
     2641 cagttgatgt tacacgcggt gacagcagtg gctctgaagg gatcaacgcc cttgccggta
     2701 gtgcaaatat gcgtactatt ggcgttgacg atgtaatatt taacggtaat acatatggcc
     2761 ttcgttcacg tttctctgtc ggtagtaatg ggctgggacg cagcggaatg atcgcccttg
     2821 gtggaaaaag cgacgctttt acggatacgg gaagcattgg cgttatggct gctgtgagcg
     2881 gcagttctgt gtactctaat ttctcaaatg gttctggaat taacagcaaa gagtttggtt
     2941 atgataaata tatgaagcag aaccccaaat cccaactgta taaaatggat atcagaccag
     3001 acgaatttaa cagcttcgaa ctttccgctc gaacctatga aaataaattt acacgtcgtg
     3061 atataaccag tgacgactat tacattaaat atcattacac ccctttttct gaattaattg
     3121 actttaacgt aacggccagt accagtcgcg gtaatcaaaa gtatcgtgat ggctcgctgt
     3181 atactttcta caaaacctca gcgcaaaatc gttctgacgc gctggatatc aacaatacca
     3241 gccggttcac tgtcgcggac aatgacctgg agtttatgct gggcagcaaa ctgatgcgta
     3301 cccgctatga ccggaccatt cactcagcgg cgggcgaccc gaaagcgaat caggaatcga
     3361 tcgagaacaa tccgttcgca ccctccggcc agcaggatat ttcagcgctg tataccgggc
     3421 tgaaggttac gcgcggcatc tgggaggcag atttcaatct caactacaca cgtaacagga
     3481 tcacagggta caagcccgcc tgcgattcac gcgttatctg cgtgccacag ggtagctacg
     3541 atattgacga taaagagggt ggcttcaacc cttcagttca gctttctgct caggtaacac
     3601 catggcttca gccgttcatt ggctacagca aatccatgcg cgccccgaac atccaggaga
     3661 tgttcttctc taattcagga ggcgcatcca tgaacccatt cctgaagcct gaacgtgcag
     3721 aaacctggca ggcgggtttt aacattgata ccagagattt actggtcgaa caggatgccc
     3781 tgcgctttaa ggctctggcg taccgcagca ggatccagaa ctacatctac agcgagtctt
     3841 atctggtttg ttctggaggt cgtaaatgca gtatgcctga ggtgattggc aatgactggg
     3901 agggcattag cgatgaatac agcgacaata tgtacatcta cgttaactcg gcaagcgacg
     3961 ttatcgcaaa gggcttcgaa ctggagatgg attatgatgc aggttttgct tttggccgac
     4021 tctctttcag ccagcagcaa acagaccagc caacctccat cgccagcacc tactttggcg
     4081 caggggatat gaccgaactg cccagaaaat acatgacgct ggatactggt gttcgcttct
     4141 tcgataacgc gttgaccctg ggcactatca taaaatacac aggcaaggct cgtcgcctgt
     4201 cgcctgattt tgagcaggac gaacataccg gcgcaataat caaacaggat ttgccgcaga
     4261 tcccaacgat tatcgatctc tatggtactt acgagtacaa ccgcaaccta acactgaaac
     4321 tttcggtaca aaacctgatg aacagagatt attcggaggc gctgaataag ctcaacatga
     4381 tgccaggtct tggtgacgag acccacccag ccaattccgc gcgtggcaga acatggatat
     4441 ttggcgggga cattcgtttc tgatgacaat ttccgctagg ttcagacaat acgttttctc
     4501 tcttatgtca attttattgc aggaacgaaa aatgaatatt ttcactttat ccaaagcacc
     4561 gctatacctg ttaatttcac tatttttacc cacgatggcc atggctatcg atccacctga
     4621 acgcgaactt tcgcgatttg ccctgaaaac gaattacctt cagtcccctg atgaaggcgt
     4681 ctatgaactg gcgtttgata atgccagtaa aaaggtgttt gcagcagtca ccgatcgtgt
     4741 aaatcgtgaa gccaataaag gctatctgta ttcgtttaat tcagattcgc tgaaagtcga
     4801 aaataaatac acgatgccat accgggcatt ttcgctggcg ataaatcagg ataaacatca
     4861 gctctatatc ggacacaccc agtcagcgtc cctgcgtatc agtatgtttg acaccccaac
     4921 cggcaaactg gtaagaacca gcgacaggtt aagttttaaa gcggcaaacg ctgcagattc
     4981 gcgttttgag cattttcgcc atatggttta cagccaggat tccgataccc tgtttgtgag
     5041 ttatagcaat atgctgaaaa cggccgaggg catgaagcct ctgcataagc tgttaatgct
     5101 cgacgggacg acgcttgcct taaaaggcga ggttaaggat gcttacaaag gtacagcgta
     5161 tggtctgacg atggatgaaa aaacacagaa aatctacgtt ggcggaagag attacatcaa
     5221 cgaaattgat gcgaaaaatc agacgctgct gcgtaccatc ccgttgaaag atccgagacc
     5281 acaaatcaca agtgtgcaga atctggcggt ggactccgct tctgaccgtg cctttgtggt
     5341 ggtattcgac catgacgatc gttccggtac aaaagatgga ctctatattt ttgacttacg
     5401 cgacggtaaa cagcttggct atgtgcacac aggagccgga gctaacgcgg tgaaatacaa
     5461 tccgaaatat aacgaactgt atgtcaccaa cttcactagc ggcaccatca gcgtagtgga
     5521 tgccaccaaa tacagcatca cccgtgaatt taacatgccg gtctacccaa accagatggt
     5581 gttgtcggac gatatggata ccctttacat tggcatcaaa gaaggcttta accgcgattg
     5641 ggatcctgat gtgtttgtgg aaggagctaa agaacgtatt ctgagcattg atttgaaaaa
     5701 gtcgtgatcg gggggaaaaa acatgggata aaaactccgt tattccacta aactggccgg
     5761 actaggaaaa gcaaacatct ctgtcctgcc ggtacgcctg ttacgccgct ccatgaggct
     5821 gaggaatctc cagaaaacga gacaggcaca gcattgtacg atcaactgat ttgtgccaat
     5881 aattaaatga aatcacagct atgcctgttc atggggttct aggccggaac cccagatttt
     5941 tccgattgtt tgttttctaa ctgattattc agtagtttta gagggctgac gctcagtgga
     6001 acgaaaactc acgttaagag gttttgacta tgataaaagt caaagtaggg gtgagtttgg
     6061 atatggaaat tattgtacta taaggagagc atgaaaaccc ctggggcagt cactgaatga
     6121 gggaccagca gcggtaaaat aaccagcata atttctgcat ggtcgatttt tataccgtga
     6181 caggctgttt tttatacttg gggtcagttt ggatatcgaa aattattgta cgttaaggtt
     6241 gttttttgac cttgtcgatc ttaactgatc tcaaacgacg aaacttacct gaagtcggta
     6301 tccggctgtt tttccagata taatcgcctg tcagattgat atgttcccaa cctaatggtg
     6361 ataaatgcga taatagttga gcatttattt tcatgccctt acgcttcaga gcatcaaccg
     6421 ctctttccat gtaaactgtg ttccataagg aaatagccgc cgtcaataaa gtcaatccac
     6481 tggcgcgata actttgattt tccggtttcc gatctctaat ctcccccaat cgatgcatga
     6541 atacagcacg ggccagagca ttacgggctt ctcctttgtt cagcccggcc tggacacgtc
     6601 gccgcaatgc cggatcccga aaccagtcca gcataaacag tgttcgttct atacggccaa
     6661 tctctctcag agctttagcc aacccatttt gtttgtaagc gtgcagcaag aaccgtattg
     6721 acgggaatgt gttattcagt cggcagtgct acgcgccagg ggagcagttc gccgacccgg
     6781 tttatcggcc agtcggctat gacgtcaagg acatagcgga ggtagctttc tggctccact
     6841 ccgttcagtt tgcacgtccc gatcaggctg tacagcagcg ctccccgctc tcctccatga
     6901 tccgaaccga agaacaggta gtttttgcgg cccagactga ccatccgcaa cgcattttca
     6961 gcgatgttat tgtccgcctc agcccagcca tcatctgcat agtacgtcag cgccggccac
     7021 tggttcaggg cgtatgcgaa cgctttcgcc agttctgagt gtcgcgacag ggttttcatc
     7081 ttttcacgca gccagctttc cagggatttc agcagcggtt tcgttttcaa ctgacgttcg
     7141 gcaaggcgct gttccgccgt cattcccctt atctctgcct cgatggcgta cagttcgccg
     7201 atccgtttca gcgcttcctc cgtcagggct gacggggtgc gaacgtgcac atcgtggatt
     7261 ttacggcggg cgtgagccca acaggcggct tccgttatcc ggccatcccg gtacagctcg
     7321 ttgaacccgg cgtatgcatc cgcctgcagt acaccactga accccgcaag atgggtctgc
     7381 ggatggatgc cttttctgtc cgggctgtaa gcgaaccaca ccgccggcgc cagcgttgac
     7441 ccggcgttac ggtcgtcacg aacatacgcc cacaaccgcc cggtcttcgt tttcttattg
     7501 cctggcaaca gcaccgggac aggcgtgtca tcaacatgga gcttaccgtc agtcagcaca
     7561 tagccatgaa gcgcctcttc catcggagac agcagccggc agcatgcatc cacccagccc
     7621 gacagcagtg aacggctcag ctccacacct tgccggccgt atatttctga ctggcgatac
     7681 agcggggtgt gctctgcata cttcgaggtc agcacgcggg ccagcagccc cggtccggcg
     7741 ataccccgct cgatgggccg cgaaggtgca ggtgcctgca cgatggcatc gcactgagta
     7801 caggcatgtt tttcccgtac cgtccggata acccggaagg cgctacgcat caactccagc
     7861 tgttcggcgg tatcctcgcc cagatagctc agtgaaccgc cgcagttcgg gcagcacggc
     7921 gccgcaggca acagtcgctt ttcgtcacgg ggtagtgatt cagggaacgg cttacgggtg
     7981 cgggtctgac gcaacggacg ctgtactgcc gggtcataca ccctaccagt cagcgtatcg
     8041 ctctctttct gaagccggtt cagatcggct tccatttgtg cgatacggcg ggagactttt
     8101 tcggaacgac tgccgaagtt catccggcgg agtttatcca gctgcgcctg cagatggtct
     8161 atttcgcgct cccggttgct aagcttttcc tgcagggcgt ggatcagcgc ttcctgttcg
     8221 gccaggcgct gtttcagcag gaagatgtcg tcagaagaga tgtcgttcat aagcccgtat
     8281 tttaccgggc ttattctgtg acaaccagga taaagagatt tacagcatgg tcagggaggt
     8341 cagcagccgc ttaggctgtc gccagtcgat accttccagc agcatcgcca gctgcgcctg
     8401 tgtaaggaac actttgccat cacgggctga cggccaggcg aagcgcccac gctccagccg
     8461 tttggtcagg aggcacagtc cgtcactggt ggaccacagc agtttaacct gactgccgct
     8521 gcggccccgg aaaatgaaaa catggccgga catgggatcg tctttcagcg ccgtctgtac
     8581 tttcgcagcc aggccgttga agccatttct catatcggtg ataccggcaa ccagccaaat
     8641 tttgatcccg gaaggtaacg ggatcatcgc ttcagttcct gtatcagcag agtcaggagc
     8701 ttttcgctga cattgccatt gaagcggagc gtcccgtgcc ggaacgttac ctcacagctg
     8761 atactgaggg ttttcgggtc ctctgcgagc gattctggct gttcggcagc tgcatcgaga
     8821 gtcacaggaa gtagctgggg gctctctg
//
LOCUS       DAJTVE010000154         8807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000154 DAJTVE010000000
VERSION     DAJTVE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..321
                     /locus_tag="OO439_003583"
     CDS             <1..321
                     /locus_tag="OO439_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6317853.1"
                     /translation="VTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGG
                     HDLNMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEIT
                     LSPL"
     gene            425..1399
                     /gene="ghrB"
                     /locus_tag="OO439_003584"
     CDS             425..1399
                     /gene="ghrB"
                     /locus_tag="OO439_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6317854.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1449..2159)
                     /locus_tag="OO439_003585"
     CDS             complement(1449..2159)
                     /locus_tag="OO439_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6317855.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            2593..2883
                     /locus_tag="OO439_003586"
     CDS             2593..2883
                     /locus_tag="OO439_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6317856.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3164..3376
                     /gene="cspA"
                     /locus_tag="OO439_003587"
     CDS             3164..3376
                     /gene="cspA"
                     /locus_tag="OO439_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6317857.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3564..3716)
                     /gene="hokA"
                     /locus_tag="OO439_003588"
     CDS             complement(3564..3716)
                     /gene="hokA"
                     /locus_tag="OO439_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6317858.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4042..6111)
                     /gene="glyS"
                     /locus_tag="OO439_003589"
     CDS             complement(4042..6111)
                     /gene="glyS"
                     /locus_tag="OO439_003589"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6317859.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6121..7032)
                     /gene="glyQ"
                     /locus_tag="OO439_003590"
     CDS             complement(6121..7032)
                     /gene="glyQ"
                     /locus_tag="OO439_003590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6317860.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7127..7426)
                     /locus_tag="OO439_003591"
     CDS             complement(7127..7426)
                     /locus_tag="OO439_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6317861.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            7601..8596
                     /gene="wecH"
                     /locus_tag="OO439_003592"
     CDS             7601..8596
                     /gene="wecH"
                     /locus_tag="OO439_003592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6317862.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(8638..>8807)
                     /locus_tag="OO439_003593"
     CDS             complement(8638..>8807)
                     /locus_tag="OO439_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317863.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2189 a         2231 c         2144 g         2243 t
ORIGIN      
        1 gtgaccttcg acagcagcag cgcgaccctg aaaccggcag gcgctaacac cctgaccggc
       61 gtggcaatgg tgctgacaga gtatccgaaa acggcggtta acgtgattgg ttataccgac
      121 agcacggggg gtcacgacct gaacatgcgt ctctcccagc agcgtgcgga ttccgttgcc
      181 agcgcgttga tcacccaggg cgtggacgcc agccgcatcc gtactcaggg ccttggcccg
      241 gctaacccaa tcgccagcaa cagcaccgca gaaggtaagg cgcaaaaccg ccgtgtagaa
      301 attaccttaa gcccgctgta atccctttca tgccaggtga tgcagaaatc acctggcatt
      361 cttcagtttt ccttcatcat atttcaggct aaggtgatcg ccttatcagt gaatggagag
      421 aagcatgaag ccgtccgtta tcctctacaa agccttacct gatgatttac tgcaacgcct
      481 gcaagagcat ttcaccgttc accaggtggc aaacctcagc ccacaaaccg tcgaacaaaa
      541 tgcagcaatt tttgccgaag ctgaaggttt actgggttca aacgagaatg ttgatgccgc
      601 attgctggaa aaaatgccga aactgcatgc cacatcaacg atctccgtcg gctatgacaa
      661 ttttgatgtc gatgcgctta ccgcccgaaa aattctgctg atgcacacgc caaccgtatt
      721 aacagaaacc gtcgccgata cgctgatggc gctggtgttg tctaccgctc gtcgggttgt
      781 ggaggtagca gaacgggtaa aagcaggcga atggaccgcg agcataggcc cggactggta
      841 cggcactgac gttcaccata aaacactggg cattgtcggg atgggacgga tcggtatggc
      901 gctggcacaa cgtgcgcact ttggcttcaa catgcccatc ctctataacg cgcgccgcca
      961 ccataaagaa gcagaagaac gcttcaacgc ccgctactgc gatttggata cactgttaca
     1021 agagtcagat ttcgtttgcc tgatcctgcc gttaactgat gagacgcatc atctgtttgg
     1081 cgcagaacaa ttcgccaaaa tgaaatcctc cgccattttc attaatgccg gacgtggccc
     1141 ggtggttgac gaaaatgcac tgatcgcagc attacagaaa ggggaaattc acgccgccgg
     1201 gcttgatgtc ttcgaacaag agccactgtc cgtagattcg ccgttgctct caatggccaa
     1261 cgtcgtcgca gtaccgcata ttggatctgc cacccatgag acgcgttatg gcatggccgc
     1321 ctgtgccgtg gataatttaa ttgatgcgtt acaaggaaag gttgagaaga actgtgtgaa
     1381 tcctcacgtc gcggactaag ccgcgactgc gtgaagtaaa gcccgataat cgctcgggct
     1441 tttactcttt attgggttgc agtaactgct gtagtccagg cctgattaaa cgcctgatgt
     1501 tgtgccggta atggcgcaat cagtttgtta tattcacttg cctgctgtga agtcgggaac
     1561 tggatgccat ttgcgacaaa gctcacctga gtaccttgct gagcaatgta gtctccaacc
     1621 atcaccagtt gttgcgtgaa agtttgtgcc gccggaatca acggttgcaa tgcatcagct
     1681 ggcgtcgtta ccactttggt gaatgcctga tcaaataccg gtttcaggtc atcgctctgt
     1741 tttagcgcag agtgcgcagc atcggcctgc aacttcgcat tttgcagttg ttgcgccagc
     1801 acacccagcg aaccattcat ttcacgcagc ggaccgcttt gcgtaacgta atcctgcggt
     1861 acgcgaattg cgttaacgct gtctacgacc gggcgcagac cagaatccat cgcctgattc
     1921 acctgctgag aataaccata cagaatcgcg taatcagaga caaaaggacc aaactgtttt
     1981 ttctgatcgg cagtcagggt tggtagacgt tcaccgctac gcatcactgt attctgcagg
     2041 aagtcgataa acgctttacg ctgatcgcct tctttatcaa aacacccact caggctaact
     2101 accattaata acgccgcaag aggcgcaaac cagcgagagc aggactttcc tgtcgccatt
     2161 ttcttactcc tttcacccaa aaaaagcgca caacgacaca cgcgtgtctg acgtgacaag
     2221 gatagtccag gacaggcttg caggataccc tttctacgta atcttcctgg taaaatcatg
     2281 gagaaaatga aacaaggtta atttgttgtt tcattgttaa aaaatgatag tgccgttgcc
     2341 ttttttgtag ccagagattt acgccttcgc agaaggttag attttcgaaa tctttacaaa
     2401 acaggagtgg taagagatat atcggcattt atcagatcgt taggtggcta taagtcacgc
     2461 taaatgacag gctgaatcga atcatagcca gagcatgccc tgacttcacc ccgctgtgtc
     2521 tgcttttccc gactattctt aatgagcttc gatgcaattc acgatcccgc agtgtgattt
     2581 gaggagtttt caatggaata taaagatcca atgcatgagc tgttgagcag cctggaacag
     2641 attgttttta aagatgaaac gcagaaaatt accctgacgc acagaacaac gtcctgtacc
     2701 gaaattgagc agttacgaaa agggacaggg ttaaaaatcg atgatttcgc ccgggttttg
     2761 ggcgtatcag tcgccatggt aaaggaatgg gaatccagac gcgtgaagcc ttcaagtgcc
     2821 gaactaaaat tgatgcgttt gattcaagcc aacccggcat taagtaagca gttgatggaa
     2881 tagactttta tccactttat tgctgtttac ggtcctgatg acaggaccgt tttccaaccg
     2941 attaatcata aatatgaaaa ataattgttg catcacccgc caatgcgtgg cttaatgcac
     3001 atcaacggtt tgacgtacag accattaaag cagtgtagta aggcaagtcc cttcaagagt
     3061 tatcgttgat acccctcgta gtgcacattc ctttaacgct tcaaaatctg taaagcacgc
     3121 catatcgccg aaaggcacac ttaattatta aaggtaatac actatgtccg gtaaaatgac
     3181 tggtatcgta aaatggttca acgctgacaa aggcttcggc ttcatcactc ctgacgatgg
     3241 ctctaaagat gtgttcgtac acttctctgc tatccagaac gatggttaca aatctctgga
     3301 cgaaggtcag aaagtgtcct tcaccatcga aagcggcgct aaaggcccgg cagctggtaa
     3361 cgtaaccagc ctgtaatctc tgcttaaaag cacagaatct aagatccctg ccatttggcg
     3421 gggatttttt tatttgtttt tcaggaaata aataatcgat cgcgtaataa aatttagtat
     3481 tatttttgtg aagaataaat ttgggtgcaa tgagaatgcg caacgccgta agaaaggcgg
     3541 gaataacttc ccgccaaaga ctcttactct ttcaaattgc aggctaaaaa tgccgccagc
     3601 tcataactct cctgtttaat atgcaattca cacagtgaat ctcttatcat ccaggtgaaa
     3661 aataaaagcg tgaaacaaat cactattaaa gaaagtaatc tatatttctg cggcattccc
     3721 agcctcctgt gttgatttcc aacgagtaag aggctaacct tatggtgttc aggcataggg
     3781 tagcctcact ttgatttata gtcaggtggg gcttttctct gtctgccttt cggtgaatac
     3841 ctgagacaaa cagtctcaag cacccgtggc tattctagct taataagttt gtttcttctc
     3901 cttgatataa tcctaaaaaa acctcataaa attaatatat gagataatct ttattcaaca
     3961 gaagattatt aaaggttgct gtattattta gcgataataa aaaaagcctg ccagatggca
     4021 ggctttttaa taacggcgtt attactgcaa cagcgaaata tccgcaacgc gcaggaacag
     4081 ttcgcgcagt ttctccagca tggtcagacg gttgagacgc aattctttgt catcaaccat
     4141 gaccatcact ttatcgaaga aagcatcaac cggctcacgc agctctgcca gttcgaccag
     4201 cgcatcctgg taacgacctt ccgcaaagta cggctccagc ttgtcacgta gcaccacaac
     4261 ctgcatcgcc agtttaattt cttccggctc tttcagggta gaggcattca cgcggtcgct
     4321 cagcacttcg tcagatttcg ccagaatgtt agatacacgt ttgttcgccg ccgccagtgc
     4381 agcagctgca tccagggtac ggaaatgcga taccgctttc atacgggcat cgaaatcagc
     4441 cggacgagtc ggacgacgcg ccagtaccgc ctggatggtg tcaacggtgt aaccttcgtc
     4501 ctgataccag gcgcggaagc gaccgagcat aaagtcgata acatcatcaa ctacgttggc
     4561 gttagtcagc ttatcgccat acagacgcac tgcttcttcg gtcagcgttt gcagatcaag
     4621 gttgaggttc ttctcaacga taattcgcag cacgccaagt gcggcacgac gcagcgcaaa
     4681 cgggtctttg tcgcctttcg gatgctgacc gataccgaag atacccgcca gagtatccat
     4741 cttatcagca atcgccagcg cacaagctac cgggttggac ggcaggtcat caccggcaaa
     4801 gcgcggctga tactgctcat tcagcgcgac ggctacgtct tccgcttcgc catcgtgacg
     4861 cgcgtagtgc atccccataa cgccttgggt gtcggtgaac tcgaagacca tgttggtcat
     4921 caggtcacac ttagacagca ggcccgcacg ggttgcgtgg ttaacgtcag cgccaatctg
     4981 ttcagcaatc cagccagcca gcgcctggat gcggtcagtt ttgtcacgca gcgtacccag
     5041 ctgttgctgg aacagcacgg tttgcaggcg cggcaggtta tcttccagac gttttttacg
     5101 gtcggtattg aagaagaact cggcatccgc cagacgtgga cgaacgactt tctcgttacc
     5161 agagataatt tgctgcggat ctttcgattc gatgttggca acgaagatga agttcggcag
     5221 cagtttgcca tcgttcgcat acaccgggaa gtatttctgg tcacctttca tggtgtaaac
     5281 cagggcttca gccggcaccg cgaggaattt ctcttcgaat tttgcggtca gaacgaccgg
     5341 ccactccacc agcgaagcca cttcttccag caggctttcg cttaagtcag cgttaccgcc
     5401 aatcttacgc gctgcttctt cggcatcggc tttaatcttc gctttacgtt cttcgtaatc
     5461 ggcgatgact ttcccgcgct cacgcagaat ttccggatac tgatcggcgt tgtcgatggt
     5521 gaattccggt tcgcccataa agcggtggcc gcgaatcacg cgatcggact gaatgcccag
     5581 aatggttgcc ggaatgactt tgtcgcccag cagcagggtc acggtgtgca ccggacgcac
     5641 gaagtgcacg tcgcttgcgc cccaacgcat cagtttcggg atcggcagtt tcgccagcga
     5701 agtcgcaacc atattcggca gtagtgcttc agtgctttcg cccttcacat gggcgcgata
     5761 cagcagccat tcgcctttat cggtagtcag acgctcagcc tggtcaacgg taataccgca
     5821 accacgcgcc caaccttctg ccgctttgct cggtttgcct tcagcgtcga acgcctgggc
     5881 aatcgccggg ccgcgttttt cgatttcacg atctggttgc gcttccgcca ggttagctac
     5941 tttcaacgcc agacgacgcg gagcagcaaa ccattgaacg gtgccgtgtg cgaggccagc
     6001 gttatccagc tccgcagtaa agttcgcagc aaaggactca gccaggctgc gcagtgcttt
     6061 tggtggcagc tcttcagtgc cgatttccac cagaaaagtt ttctcagaca tagccgcctc
     6121 ttacttatct ttgttgcaca tcgggaagcc gagggcttca cgggaagcgt agtatgcttc
     6181 tgccactgct ttggtcaggg tgcgaatgcg cagaatgtag cgctgacgct cagtgacgga
     6241 gatggcttta cgcgcatcca gcaggttgaa gctgtgggcg gctttcagaa tacgctcgta
     6301 ggctggcagc ggcagcggat tttccagcgc cagcagctgc tgcgcttctt tctcgtactg
     6361 ctcgaagcag gtgaacagga aatccacatc cgcgtattcg aagttgtaag tggactgctc
     6421 cacttcgttc tgatggaaca cgtcgccgta ggtggtttta cccagcgggc cgtcgctcca
     6481 gaccaggtcg taaacgctgt ctacgccctg aatgtacatg gccagacgtt ccagaccgta
     6541 ggtgatctcg ccggtaaccg gtttacactc cagaccacca acctgctgga agtaagtgaa
     6601 ctgcgtcact tccatgccgt ttagccacac ttcccagccc agtccccagg cacccagagt
     6661 cgggttttcc cagttatctt ccacgaaacg gatgtcgtga atagtcgggt ccatgcccag
     6721 ctctttcaga gaaccgaggt acagctcctg aatattgtcc ggcgatggct taatgaccac
     6781 ctggaactga tagtagtgct gtaaacggtt ggggttttcg ccgtagcgac catcggtcgg
     6841 gcgacgagaa ggctgaacat aagcagccgc catcggttct ggccccagcg cgcgcagaca
     6901 ggtcattggg tgagaggttc ccgcgccgac ttccatgtcc aatggttgaa caatggtgca
     6961 gccctggcga gcccagtaat cctgtaaggt caggatcaag ccctggaagg tcctggtatc
     7021 aaacttttgc atattatttc gtgctggata cgtgtggatt taaaggaagg gatcagtata
     7081 cccgctggat ggaagatata cagtacgaaa cgggaaaaag cagggcttaa cgcatggaaa
     7141 gatgcaaaaa atggccatct gcgtcaaaag aacagacaaa ctgctcttta cggaaggtat
     7201 aaccgcgcat ttcatagctt ccctggaact gttcgaaggc ggtgacgtcg attttttgct
     7261 cgccggtgtt ataacgtttt gccgcctgat ccttgcataa cgcttccata ttcaacgaac
     7321 gcagaggatc aacctttacc cgttgtgctt tctgcacggg tgacggtgta gaccaaccca
     7381 ctagcaccat aagcgccatt gccggaaaga aagcgttcat catcattaaa ttaccgtctg
     7441 gtttgcaggt gagtcttatt atttatatcg gtatagtggc tgatatctta cgaatttcct
     7501 taagcctcta taagctgtag tgataaaact cagattattc cagtgcgcca accaaatgat
     7561 ttatcggtca gacttttttt aaccagatac agagacacag atgcagccca aaatttactg
     7621 gattgataac ctgcgaggga tagcgtgttt aatggtggtg atgattcaca ccactacctg
     7681 gtatgtgacc aatgctcata gtgttagccc cgtcacatgg gatatcgcca atgttctgaa
     7741 ttctgcctct cgtgtcagcg tgccgctatt tttcatgatt tccggctatc tcttttttgg
     7801 cgaacgcagc gcccagccgc gccatttctt gcgtatcggc ttatgtctga ttttttatag
     7861 cgcaatcgca ctgctctaca ttgcgctgtt tacctccatc aatatggagt tagcgctgaa
     7921 aaacctgctg caaaagccag tgttttacca cttgtggttt ttcttcgcga ttgcggtgat
     7981 ttatctggtt tcaccgctga ttcaggtgaa gaacgtcggc ggaaaaatgt tgctggtgct
     8041 aatggcggtg attggcatta tcgctaaccc aaacacagtg ccgcagaaaa ttgacggttt
     8101 tgaatggctg ccaattaact tatatatcaa tggcgatact ttttactaca ttctgtatgg
     8161 catgttgggc cgcgctatag ggatgatgga cacacagcat aaagcactgt cgtgggtgag
     8221 cgccgcgctg tttgcgacgg gggtttttat tatctctcgc gggacattat atgaattgca
     8281 gtggcgcgga aattttgccg atacctggta tctttactgt gggccgatgg tttttatctg
     8341 cgcaatcgcg ctattgactc tggttaaaaa cacgctggat acgcgtacca ttcgcggact
     8401 tggcttaatc tcccgccatt cattgggtat atacggattc cacgccttga ttatccatgc
     8461 gctgcgcacc cggggaattg agcttaaaaa ttggccaata ctggatatta tttggatctt
     8521 ttgcgcgacg ttggcagcga gtttgttact ttctatgctg gtacaacgaa tcgacagaaa
     8581 cagattagtg agttaagtaa aagcccgttc acattggact gaccgggctt acgtgaatta
     8641 ttcgctgtat tcttcctggg taacctgtgt ttcttttggc gggtttattc cggcatcacg
     8701 aatattcttc tgcaccgcta ctgcaccaaa caagcttaag aagaaagcca gtccataaaa
     8761 acctttttca ctgagtaata atgtcgcatt ccacagacct accatca
//
LOCUS       DAJTVE010000155         8670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000155 DAJTVE010000000
VERSION     DAJTVE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            812..1054
                     /locus_tag="OO439_003594"
     CDS             812..1054
                     /locus_tag="OO439_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317864.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO439_003595"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO439_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6317865.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO439_003596"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO439_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6317866.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO439_003597"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO439_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6317867.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO439_003598"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO439_003598"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6317868.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="OO439_003599"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="OO439_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6317869.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8670
                     /locus_tag="OO439_003600"
     CDS             8087..>8670
                     /locus_tag="OO439_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6317870.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLES"
BASE COUNT         2058 a         2201 c         2230 g         2181 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt
//
LOCUS       DAJTVE010000156         8577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000156 DAJTVE010000000
VERSION     DAJTVE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1161
                     /gene="rtcR"
                     /locus_tag="OO439_003601"
                     /pseudo
     CDS             1..1161
                     /gene="rtcR"
                     /locus_tag="OO439_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1143..1901)
                     /locus_tag="OO439_003602"
     CDS             complement(1143..1901)
                     /locus_tag="OO439_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6317871.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(1918..2748)
                     /gene="glpG"
                     /locus_tag="OO439_003603"
     CDS             complement(1918..2748)
                     /gene="glpG"
                     /locus_tag="OO439_003603"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6317872.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(2793..3119)
                     /gene="glpE"
                     /locus_tag="OO439_003604"
     CDS             complement(2793..3119)
                     /gene="glpE"
                     /locus_tag="OO439_003604"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6317873.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3309..4814
                     /gene="glpD"
                     /locus_tag="OO439_003605"
     CDS             3309..4814
                     /gene="glpD"
                     /locus_tag="OO439_003605"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6317874.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(4866..5471)
                     /locus_tag="OO439_003606"
     CDS             complement(4866..5471)
                     /locus_tag="OO439_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317875.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5474..6241)
                     /locus_tag="OO439_003607"
     CDS             complement(5474..6241)
                     /locus_tag="OO439_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317876.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6244..6954)
                     /locus_tag="OO439_003608"
     CDS             complement(6244..6954)
                     /locus_tag="OO439_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317877.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(6970..8475)
                     /locus_tag="OO439_003609"
     CDS             complement(6970..8475)
                     /locus_tag="OO439_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6317878.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2215 a         2217 c         2118 g         2027 t
ORIGIN      
        1 cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg atttaagccg ttataacgcc
       61 atcgccagcc gctttgccga ggaacgccag caaacgcttg attttcttaa gtccggcatt
      121 gccacgcgta acccccactt caaccgcatg attgagcaga tcgaaaaagt ggcgatcaaa
      181 tcccgcgcgc cgattctgct taacggtcca accggcgcgg gcaagtcatt tctggcgcga
      241 cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg gcgcgtttgt ggaagtgaac
      301 tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt ttggtcatgt aaaaggcgcg
      361 tttaccgggg cgcggtaatc tcgtgaaggt ttattacgca gcgccaacgg cggaatgttg
      421 tttcttgatg agattggcga actgggcgca gacgaacagg caatgctgct aaaagccatt
      481 gaagagaaaa ccttttaccc gtttggcagc gatcgccagg tgagcagtga ttttcagctt
      541 atcgccggaa cggtgcgcga tttgcgccag ctggttgccg aaggcaaatt tcgcgaagat
      601 ctgtacgcgc ggatcaatct ctggaccttc accctgccgg gtctacgcca gcgccaggaa
      661 gatattgaac cgaaccttga ttatgaagtg gagcgccacg ccacactcac tggcgacagc
      721 gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt ttgcgacctc tccccaggca
      781 acatggcgcg gtaactttcg cgaactttct gccagcgtca cgcggatggc cacctttgcc
      841 actagcggac gcatcactct ggacgtggtt gaagatgaga taaaccgtct gcgctataac
      901 tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg gcgcagaggc agaaaacatc
      961 aatctcttcg accgtatgca actggaacac gttatcgcta tctgccgcta ggcaaagtcg
     1021 ctttctgcag ccggacgaca gctttttgat gtttcgcgcc agggcaaagc cagcgtcaat
     1081 gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacgtggga agccgtgcag
     1141 gatcagcaca gctccagttg aatatgatgg tccgtcagca cctgcattac gcttactggc
     1201 ggcggggcgt cggtgtagac ggcatctacc atgctgatgc tgcccatatt gaccatcgcg
     1261 ttacggccaa atttcgagtg atcgacaacc agcataacgt ggcgcgagtt ctcaataatg
     1321 gcgcgcttgg tgcgaacttc gtggtaatcg aactccagca gcgagccgtc gctatcgatg
     1381 ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc gagcgtcgct
     1441 tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcgagaat aatgcggaaa
     1501 tcttctttta ccatcaacgt gttagcaaca ttgagattgt tggtgacaat acgcaaattg
     1561 ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc gataaacagt
     1621 gtcgagccgt tggggatttg ctccgccact ttgcgggcaa tcctctcttt ttcttcggtc
     1681 tgggtggctt tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag cgccgcaccg
     1741 ccatggtgac gcaggatcag gttttgctcc gccagctcat tgaggtcgcg gcgaatagtc
     1801 tgcgggctga cggagaaatg ctctaccagc tcttcagtac tgacataacc ctgctgttta
     1861 accagttcga taataccgtt gtgacgttgt gtttgtttca tttataaatc cctggaatta
     1921 ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca cggctaaccc
     1981 ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc cggcgacaat
     2041 ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa atgccacttt gcggatcgcg
     2101 ttcaccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa gcccgccaaa
     2161 ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa tgagagtaat
     2221 aacaattagc ttaccgctac cgaggcgttt ttccaccgca ccgccgagat accaccacca
     2281 gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg tgaagtaacg
     2341 ccagaactca aatttcagtg ctggatcgaa tggccaggcc agccataaca tcacttcctg
     2401 atcgccgaga atttgcatgg caataaacac caccacgcag gcgatcatca tcacccaggt
     2461 caccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat aatgcaggcc
     2521 actgccggta tggcctgcct gccagctggc cgccagataa cgcggatctg ccgggttttc
     2581 gagaaaacgc gccagctccg cccgtacgcg ctcggcctgg gactcatccg ccagccagac
     2641 atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgcgtcgcca tgtaatcaac
     2701 aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc atcaacatcg ttgctgtcgc
     2761 ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac ctctgcggga
     2821 aactgacgtt gccaggcttc aaagccgccg tcaatgctat agaccacatc gtagccctgt
     2881 tgcagcagat actgcgccgc gcctttgctg ctattgccgt gataacacat caccatcacc
     2941 ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg tgtcgttggt taaatggaaa
     3001 gcctgcaccg catgccccat agcgaaactc tgtggatcgc gaatatcgac cagcaccgcc
     3061 tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa ctgatccatg
     3121 cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg tcgaagcggc
     3181 agataaacgc cataatgtta tacatatcac tctaaaatgt tttttcaatg ttacctaaag
     3241 cgcgattctt tgctaatatg ttcgataacg aacatttatg agctttaacg aaagtgaatg
     3301 agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat ggtgctggta
     3361 tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg caggatctcg
     3421 cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc taccttgagc
     3481 actatgaatt ccgcctggtc agcgaggcgc tggctgaacg tgaagtgctg ctgaaaatgg
     3541 ctccgcatat cgccttcccg atgcgttttc gcctgccaca tcgtccgcat ctgcgcccgg
     3601 cgtggatgat tcgcattggt ctgtttatgt acgatcatct gggtaaacgc accagcctgc
     3661 cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt aaaaccggaa attaagcgcg
     3721 gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc aacgcccaga
     3781 tggtggtgcg taaaggcggc gaagtgctta ctcggactcg cgccacctct gctcgccgcg
     3841 aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa tatagctggc
     3901 aagcgcgcgg cttggttaac gccaccggcc cgtgggtgaa acagttcttc gacgacagga
     3961 tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt gtggtgccgc
     4021 gcgtgcatac ccaaaagcag gcctacattc tgcaaaacga agataaacgc attgtgttcg
     4081 tgatcccgtg gatggacgag ttttccatca tcggcaccac cgatgtggaa tacaaaggcg
     4141 atccgaaagc ggtgaagatt gaagagagtg aaatcaatta cctgctgaaa gtgtataaca
     4201 cgcactttaa aaagcagtta agccgtgacg atatcgtctg gacctactcc ggtgtgcgtc
     4261 cgctgtgtga tgatgagtcc gactcgccgc aggctattac ccgtgattac acccttgata
     4321 tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt cggcggtaag ttgaccacct
     4381 accgcaaact ggcggaacat gcgctggaaa aactgacgcc gtattatcag ggcattggcc
     4441 cggcatggac gaaagagagc gtgctgccgg gtggtgccat tgaaggcgac cgcgacgatt
     4501 acgcagctcg tctgcgccgt cgctatccat tcctgactga atcgctggcg cgtcattacg
     4561 cacgcactta cggcagcaac agcgagctgc tgctcggcaa tgcgggggcg ataagcgacc
     4621 tcggggagga tttcggtcat gagttctacg aagcggaact gaaatacctg gtggatcacg
     4681 aatgggtccg ccgcaccgac gatgcccttt ggcgtcgcac aaaacaaggc atgtggctga
     4741 atgcggatca acaatctcgt gtgagtcagt ggctggtgga atatacgcag cagaagttgt
     4801 cgctggcgtc gtaaggatga tgcccggttg aagtgcaacc gggctatttg gattccttag
     4861 actattcatt gtaaataatt gaataacggc ccctgagcta aaatcgccaa acttaagcac
     4921 gttacccaat cgccccactt cttcctctgg tttctatttt ttctgcacca atggcaataa
     4981 tatcccaatg caaaaccaag ctgtgcacta atcgggccga caaaatcagt ccaaggctgc
     5041 ggccaaaaag gggaaaaatt caggggaggc cagacaaacg tcagaaagaa atttcggata
     5101 ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga gagaattaac
     5161 cagaaataga ccggcgaaaa gaccagccag caatgcagtc tgttttttaa ttcaggaggc
     5221 gttctgcggg agaattgcca accgatgcca gccattacac tgaaaaaaag tgcatattca
     5281 accattaact caagttgtaa ttcctgaaga gaaatacgca caaccaggag cggaaaggag
     5341 aatattattt ctgccagaaa agttaacgtg aatataagca cggctaagac aataaccgtc
     5401 ggccatacca ataatggcac gataacaaaa ccgagaatta cagggggaat ataaagccgg
     5461 aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc gcgcataaac
     5521 actcatcaat tatccagtca tcttcccaga cctgatattc tgtcattggt tcgggctgtt
     5581 ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag taaaggatga ttatttctag
     5641 cccaggcatt gatttgggtg gtaatggcgt tatcgatatc gtcctggtta tctctgatat
     5701 aatcctcaca atatctgttc aataaacagg caagagaaac ggtcgaccaa aagccgggaa
     5761 ctgaattaat ggcatccagc aatggcgcaa gagcgatatc ggcacggaaa aacatttcca
     5821 gcatatactg taattcgaca gcttcatcgc cggataccca atcagtcata tactggcagg
     5881 caaatcgctg taaaattgcc cgttcatccg cacgccagtt ttctttgttg gcaaggttga
     5941 tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg cccctgtgca acaaattcca
     6001 gtaaacgcgg cagaaaatag cggacctcat cgctattttg cggatcaggc cctgggctac
     6061 tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtactc cgtaatgcct
     6121 tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc ggcacccgat
     6181 aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg catggcgggg ctgggttgca
     6241 tagttacccc ctcgcccgct gatcgcgtgt aaacggttga tagacaaaac gagcattcgc
     6301 cgcgttcagg cgaataaggg cgctacgcga aagggaaaag ctggggcgaa tgcaactatt
     6361 gcagtctcga cagctttcct ctgcgcaatt ttccactgag taatgacgcg tgtagagttt
     6421 cagttcggcc tgcttatcgc gaaatccgcg taaactgtta ttgttatgaa atttccaggc
     6481 atctttaaag acctgaaaga tatagacatc ctcaatccag gagtcacatt ttttaagcgg
     6541 gcgctttgct attatcaccc gctcatccgg atttagcatc gccgtaaatt gtacggtgac
     6601 gtcatttgtc gtgttcaggt cacattgaac atctggtaca tcactgatat aggggtcaaa
     6661 tccaaaatca gcaccacagg cacaaagacg gctaatggtg gcggtatcga gggaaaatcc
     6721 cggccatacc ttgctggagg ttaagccaat atttaatgtg acatcatatc gaagcaatcc
     6781 ctcaagaccg tcagcaccga cgaacttttc cccttcatcc aaggcctggt taataagttg
     6841 ctgaacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa tatccacgcc
     6901 tgtgaaggga tcagtaccgg caaggcttgc aaataatgta ttaactaatt ccatttttat
     6961 tatccagctt taaggtagcg tcttatacat cgggacataa cctacatctt ccaccaaact
     7021 ctgtccctgc ggcgttaaaa accactccaa caatttttgt gtctctgacg tcgtattttc
     7081 ccgcgttacc ataaaggcat cgacgatata gggatatttg ccgttgcgaa tgttttcggc
     7141 agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcggcgt tcatttgtgt
     7201 tgcgtaataa cggaaggtgt aacctattgc gttgtttgta ttacggtatt cggcaacgac
     7261 tttaatcatt ccctccatca tacttgccac ttctgtttcc tgcggcgaga tcatgcggac
     7321 atttttcatg acctgggatt gcatcaccgt ctggctacca gagtcttccg ggcgttgcca
     7381 ggtctggatc tcctgatcgt taccgccaac agtacgccag ttggtaattg caccactgaa
     7441 gatgtcacgc acctgttgtt cagtcaggga attaaccgga ttatccgcat tgacgatgaa
     7501 aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta acgcccgatt cctcagcgcg
     7561 ttttttctgc ccaccggaag gttgcgccac aaaaataata tcagcatcac ctttaacaat
     7621 cctgttatac gcatcggggg tacgagagct ctccagatat tccctggtgt gaaaatcttc
     7681 cggtataacg cttaaagcat aaaatgcaga ggcataaatt gggtacgccg ccgtagcacc
     7741 atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt ttaccacgca gtggtgtcag
     7801 ttgattattg agtttatcgg gtcgccaggc ccaggtatca atattttcgc gcacaccgac
     7861 accgggattg agctgtttat ataaataacc ctgataacca gcgacaaaac caagcatgag
     7921 aaggataaac aggagggaat tacggcaacg tagcccggat gtaccggtta aaggatgtct
     7981 ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg ccaacgggta taatgactat
     8041 ccaccagtac tcacctccga ctactgagac taacaaaacg cccagccagg ggagcataag
     8101 atcagccaac caacccgatc ccatctggtg aaattgataa tctgccagat gcagcgcgat
     8161 tatccagcca accaggatta ataaaccggg tactaaaaaa agacattgtc gccagacggc
     8221 tatttctctt ttttctggtg cactatcgcc tgcccatact cgccccgaga aaaatagcat
     8281 taatgcgccc agcgccagca taaaaatgtt gagcagaagg taacttgttg gcgtgataag
     8341 gatattacaa acttcaataa taccggcaag tccgacaccc agcatagcaa caaccgccgc
     8401 caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt accagcgagg acaaaatcca
     8461 ttttctgttt tgcattattc tttccattct ttttgaatgg tgaaattata ctccccgagt
     8521 ccccttgccc cttctggaca cttttccgaa atgatggcgg aaaaaaacgg gaccctt
//
LOCUS       DAJTVE010000157         8413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000157 DAJTVE010000000
VERSION     DAJTVE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..369)
                     /locus_tag="OO439_003610"
     CDS             complement(<1..369)
                     /locus_tag="OO439_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR6317879.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNG"
     gene            648..1601
                     /gene="tal"
                     /locus_tag="OO439_003611"
     CDS             648..1601
                     /gene="tal"
                     /locus_tag="OO439_003611"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6317880.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1716..2303
                     /gene="mog"
                     /locus_tag="OO439_003612"
     CDS             1716..2303
                     /gene="mog"
                     /locus_tag="OO439_003612"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6317881.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2338..2904)
                     /gene="satP"
                     /locus_tag="OO439_003613"
     CDS             complement(2338..2904)
                     /gene="satP"
                     /locus_tag="OO439_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6317882.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3053..3766)
                     /gene="msyB"
                     /locus_tag="OO439_003614"
     CDS             complement(3053..3766)
                     /gene="msyB"
                     /locus_tag="OO439_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6317883.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(3792..4196)
                     /locus_tag="OO439_003615"
     CDS             complement(3792..4196)
                     /locus_tag="OO439_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6317884.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4573..6489
                     /gene="dnaK"
                     /locus_tag="OO439_003616"
     CDS             4573..6489
                     /gene="dnaK"
                     /locus_tag="OO439_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6317885.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6578..7708
                     /gene="dnaJ"
                     /locus_tag="OO439_003617"
     CDS             6578..7708
                     /gene="dnaJ"
                     /locus_tag="OO439_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6317886.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(7812..7964)
                     /gene="mokC"
                     /locus_tag="OO439_003618"
     CDS             complement(7812..7964)
                     /gene="mokC"
                     /locus_tag="OO439_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6317887.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2216 a         2064 c         2161 g         1972 t
ORIGIN      
        1 tccattgacg tcacgttctt tataaagttg tgcaagggaa cattcggcaa acgaggtcgc
       61 catgccgata aacgcggcaa cccacatcca aaagacggct ccaggtccac cggcggtaat
      121 agccagcgca acgccggcca ggttgccgct acccacgcgc gccgcaagac tggtacacaa
      181 tgactgaaat gaggttaaac cgcctggctg tggatgaatg ctatttttaa gacttttgcc
      241 aaactggcgg atgtagcgaa actgcacaaa tccggtgcga aaagtgaacc aacaacctgc
      301 gccgaagagc aggtaaatca ttaccgatcc ccaaaggacg ctgttaataa aggagaaaaa
      361 atctggcatg catatccctc ttattgccgg tcgcgatgac tttcctgtgt aaacgttacc
      421 aattgtttaa gaagtatata cgctacgagg tacttgataa cttctgcgta gcatacatga
      481 ggttttgtat aaaaatggcg ggcgatatca acgcagtgtc agaaatccga aacagtctcg
      541 cctggcgata accgtcttgt cggcggttgc gctgacgttg cgtcgtgata tcatcagggc
      601 agaccggtta catcccccta acaagctgtt taaagagaaa tactatcatg acggacaaat
      661 tgacctccct tcgtcagtac accaccgtag tggccgacac tggggacatc gcggcaatga
      721 agctgtatca accgcaggat gccacaacca acccttctct cattcttaac gcagcgcaga
      781 ttccggaata ccgtaagttg attgatgatg ctgtcgcctg ggcgaaacag cagagcaacg
      841 atcgcgcgca gcagatcgtg gacgcgaccg acaaactggc agtaaatatt ggtctggaaa
      901 tcctgaaact ggttccgggc cgtatctcaa ctgaagttga tgcgcgtctt tcctatgaca
      961 ccgaagcgtc aattgcgaaa gcaaaacgcc tgatcaaact ctacaacgat gctggtatta
     1021 gcaacgatcg tattctgatc aaactggctt ctacttggca gggtatccgt gctgcagaac
     1081 agctggaaaa agaaggcatc aactgtaacc tgaccctgct gttctccttc gctcaggctc
     1141 gtgcttgtgc ggaagcaggc gtgttcctga tctcgccatt tgttggccgt attcttgact
     1201 ggtacaaagc gaatactgat aagaaagagt acgctccggc agaagatccg ggcgtggttt
     1261 ctgtatctga aatctaccag tactacaaag agcacggtta tgaaaccgtg gttatgggcg
     1321 caagcttccg taacatcggc gaaattctgg aactggcagg ctgcgaccgt cttaccatcg
     1381 caccggcact gctgaaagag ctggctgaga gcgaaggggc tatcgaacgt aaactgtctt
     1441 acaccggcga agtgaaagcg cgtccggcgc gtatcactga gtccgagttc ctgtggcagc
     1501 acaaccagga tccaatggca gtagataaac tggcggaagg tatccgtaag tttgctattg
     1561 accaggaaaa actggaaaaa atgatcggcg atctgctgta atcattctta gcgtgaccgg
     1621 gaagtcggtc acgctacctc ttctgaagcc tgtctgtcac tcccttcgca gtgtatcatt
     1681 ctgtttaacg agactgttta aacggaaaaa tcttgatgaa tactttacgt attggcttag
     1741 tttccatctc tgatcgcgca tccagcggcg tttatcagga taaaggcatc cctgcgctgg
     1801 aagaatggct gacatcggcg ctaaccacgc cgtttgaact ggaaacccgc ttaatccccg
     1861 atgagcaggc gatcatcgag caaacgttgt gtgagctagt ggatgaaatg agttgccatc
     1921 tggtgctcac cacgggcgga actggcccgg cgcgtcgtga cgtaacgccc gatgcgacgc
     1981 tggcagtagc ggaccgcgag atgcctggct ttggtgaaca gatgcgccag ataagcctgc
     2041 attttgtacc aactgcgatc ctttcgcgtc aggttggtgt gattcgcaaa caggcgctga
     2101 tccttaactt acccggtcag ccgaagtcta ttaaagagac gctggaaggt gtgaaggacg
     2161 ctgcgggtaa cgttgtggta cacggtattt ttgccagcgt accgtactgc attcagttgc
     2221 tggaagggcc atacgttgaa acggcaccag aagtggttgc tgcattcaga ccgaagagtg
     2281 caagacgcga cgttagcgaa taaaaaaatc cccccgagcg gggggatctc aaaacaatta
     2341 gtgggattca ccaatcggca gaacggtgcg accaaactgc tcgttcagta cttcacccat
     2401 cgccagatag attgcactgg caccacagat cagaccaatc cagccggcaa agtggatgat
     2461 tgcggcgtta ccggcaatgt taccgatcgc cagcagggca aacagcacag tcaggctaaa
     2521 gaaaacgaat tgcagaacgc gtgcgccttt cagcgtgccg aagaacataa acagcgtaaa
     2581 tacgccccac agacccaggt agacaccaag gaactgtgca tttggcgcat cggtcagacc
     2641 cagtttcggc atcagcagaa tcgcaaccag cgtcaaccag aaagaaccgt aagaggtgaa
     2701 tgcggttaaa ccgaaagtgt tgcctttttt gtactccagc agaccggcaa aaatttgcgc
     2761 gatgccgccg tagaaaatgc ccatggcaag aataataccg tccagagcga aataacccac
     2821 gttgtgcagg ttaagcagaa tggtggtcat gccgaagccc atcaggccca gcggtgccgg
     2881 attagccaac ttagtgttgc ccataattcc tcaaaaatca tcatcgaatg aatggtgaaa
     2941 taatttccct gaataacagt agtgttttca gggcgcggca taataatcag ccagtggggc
     3001 agtgtctacg atcttttgag gggaaaatga aaattttccc cggtttacgg tgtcagacct
     3061 gagtggcgct aaccatccgg cgcaggcagg cgatttgtat tacggctgga attgtcacgc
     3121 gataggcact gccgctgacc gctttaaccc catttaatgc tgcgcccaca gggcctccca
     3181 gacccgcgcc gcgcagcaaa ccatgcccaa gcacgctcat tgctgcgtgg gtgcgtaaaa
     3241 tgcgggtcaa ttggctggaa agcaaatgcg acacgccttt tgccaataat ttgtctttca
     3301 tcagcagcgg cagcagctct tccagctcat tcaccctggc atcgaccgcg tgcagaaact
     3361 cctgcttatg ttcctcgtcc atattcttcc aggtgttacg cagaaattgt tccagtaact
     3421 gttgctcaat ctcaaacgta gacatctctt tgtcggcttt cagcttcaat cgttttgaaa
     3481 catcgagcaa aatggcccga tacaatttac cgtgtccgcg cagtttgttg gcgatactat
     3541 cgccaccaaa atgctgtaat tctccagcaa tcagctgcca gttgcggcga tgttgctcgg
     3601 gatgcccttc catcgattta aacagttcgt tgcgcatcag tacgctggag aggcgagttt
     3661 tgcctttttc attatgggtg agcaatcggg cgaaatttgc caactgttcc tcactacaat
     3721 gctgaagaaa atccagatct gaatcattca ggtaattaac attcattttt tgtggtttct
     3781 atattctggc gttagtcgtc gccgataatt ttcagcgtgg ccatatccga tgagttcacc
     3841 gtatgaccgg aaaaggtgat ttttgagacg cagcgtttat tgtcgttatc actgttaatg
     3901 ttgatccagt cagtggtttg cccttctttt atttctgaag gaatattcag actctgactg
     3961 gcgctacggg cggctttgaa ataaaccgat gcaccgctta actgtaaatc gccatggtcg
     4021 gcagagagtt gtatgcgttt cacaatgcga caaacaggaa gtttcagcgc cagatcgttg
     4081 gtttcgttac gcggcattgc aatgacgccg aggagtttat ggtcgtttgc ctgcgccgtg
     4141 cagcacagca tcaggctaat cgccaggctg gcggaaatcg taaaaacgga tttcataagg
     4201 attctcttag tgggaagagg tagagggatg aatacccact agtttactgc tgataaagag
     4261 aagattcagg tacgtaatct tttcttttta ttacaatttt ttgatgaatg ccttggctgc
     4321 gattcattct ttatatgaat aaaattgctg tcaattttac gtcttgtcct gccatatcgc
     4381 gaaatttctg cgcaaaagca caaaaaattt ttgcatctcc cccttgatga cgtgggttac
     4441 gaccccattt agtagtcaac cgcagtgagt gagtctgcaa aaaaatgaaa ttgggcagtt
     4501 gaaaccagac gtttcgcccc tattacagac tcacaaccac atgatgaccg aatatatagt
     4561 ggagacgttt agatgggtaa aataattggt atcgacctgg gtactaccaa ctcttgtgta
     4621 gcgattatgg atggcaccac tcctcgcgtg ctggagaacg ccgaaggcga tcgcaccacg
     4681 ccttctatta ttgcctatac ccaggatggt gaaactctgg ttggtcagcc ggctaaacgt
     4741 caggcagtga cgaacccgca aaacaccctg tttgcgatta aacgcctgat tggccgccgc
     4801 ttccaggacg aagaagtaca gcgtgatgtt tccatcatgc cgttcaaaat tattgctgct
     4861 gataacggcg acgcatgggt cgaagttaaa ggccagaaaa tggcaccgcc gcagatttct
     4921 gctgaagtgc tgaaaaaaat gaagaaaacc gctgaagatt acttgggtga accggtaact
     4981 gaagctgtta tcaccgtacc ggcatacttt aacgatgctc agcgtcaggc aaccaaagac
     5041 gcaggccgta tcgctggtct ggaagtaaaa cgtatcatca acgaaccgac cgcagctgcg
     5101 ctggcttacg gtctggacaa aggtactggc aaccgtacta tcgcggttta tgacctgggt
     5161 ggtggtactt tcgatatttc tattatcgaa atcgacgaag ttgacggcga aaaaaccttc
     5221 gaagttctgg ctaccaacgg tgatacccac ctggggggtg aagacttcga cagccgtctg
     5281 atcaactacc tggttgaaga attcaagaaa gatcagggca ttgacctgcg caacgatccg
     5341 ctggcaatgc agcgcctgaa agaagcggca gaaaaagcga aaatcgaact gtcttccgct
     5401 cagcagaccg acgttaacct gccgtacatc actgcagatg cgaccggtcc gaaacacatg
     5461 aacatcaaag tgactcgtgc gaaactggaa agcctggttg aagatctggt aaaccgttcc
     5521 attgagccgc tgaaagttgc actgcaggac gctggcctgt ccgtatctga tatcgacgac
     5581 gttatcctcg tgggtggtca gactcgtatg ccaatggttc agaagaaagt tgctgagttc
     5641 tttggtaaag agccgcgtaa agacgttaac ccggacgaag ctgtagcaat cggtgctgct
     5701 gttcagggtg gtgttctgac tggtgacgta aaagacgtac tgctgctgga cgttaccccg
     5761 ctgtctctgg gtatcgaaac catgggcggt gtgatgacga cgctgatcgc gaaaaatacc
     5821 actatcccga ccaagcacag ccaggtgttc tctaccgctg aagacaacca gtctgcggta
     5881 accatccatg tgctgcaggg tgaacgtaaa cgtgcagctg ataacaaatc tctgggtcag
     5941 ttcaacctgg atggtatcaa cccggcaccg cgcggcatgc cgcagatcga agttaccttc
     6001 gatatcgatg ctgacggtat cttgcacgtt tccgcgaaag ataaaaacag cggtaaagag
     6061 cagaagatca ccatcaaggc ttcttctggt ctgaacgaag atgaaatcca gaaaatggta
     6121 cgcgacgcag aagctaacgc cgaagctgac cgtaagtttg aagagctggt acagactcgc
     6181 aaccagggcg accatctgct gcacagcacc cgtaagcagg ttgaagaagc aggcgacaaa
     6241 ctgccggctg acgacaaaac tgctatcgag tctgcgctga ctgcactgga aactgctctg
     6301 aaaggtgaag acaaagccgc tatcgaagcg aaaatgcagg aactggcaca ggtttcccag
     6361 aaactgatgg aaatcgccca gcagcaacat gcccagcagc agactgccgg tgctgatgct
     6421 tctgcaaaca acgcgaaaga tgacgatgtt gtcgacgctg aatttgaaga agtcaaagac
     6481 aaaaaataat cgccctataa acgggtaatt atactgacac gggcgaaggg gaatttcctc
     6541 tccgcccgtg cattcatcta gggacaattt aaaaaagatg gctaagcaag attattacga
     6601 gattttaggc gtttccaaaa cagcggaaga gcgtgaaatc aaaaaggcct acaaacgcct
     6661 ggccatgaaa taccacccgg accgtaacca gggtgacaaa gaggccgagg cgaaatttaa
     6721 agagatcaag gaagcttatg aagttctgac cgactcgcaa aaacgtgcgg catacgatca
     6781 gtatggtcat gctgcgtttg agcaaggtgg catgggcggc ggcggttttg gcggcggcgc
     6841 agacttcagc gatatttttg gtgacgtttt cggcgatatt tttggcggcg gacgtggtcg
     6901 tcaacgtgcg gcgcgcggtg ctgatttacg ctataacatg gagctcaccc tcgaagaagc
     6961 tgtacgtggc gtgaccaaag agatccgcat tccgactctg gaagagtgtg acgtttgcca
     7021 cggtagcggt gcaaaaccag gtacacagcc gcagacctgt ccgacctgtc atggttctgg
     7081 tcaggtgcag atgcgccagg gatttttcgc tgtacagcag acctgtccac actgtcaggg
     7141 ccgcggtacg ctgatcaaag atccgtgcaa caaatgtcat ggtcatggtc gtgttgagcg
     7201 cagcaaaacg ctgtccgtta aaatcccggc aggggtggac actggagacc gcatccgtct
     7261 tgcgggcgaa ggtgaagcgg gcgagcatgg cgcaccggca ggcgatctgt acgttcaggt
     7321 tcaggttaaa cagcacccga ttttcgagcg tgaaggcaac aacctctatt gcgaagtccc
     7381 gatcaacttc gctatggcgg cgctgggtgg cgaaatcgaa gtaccgaccc ttgatggtcg
     7441 cgtcaaactg aaagtgcctg gcgaaaccca gaccggtaag cttttccgta tgcgcggtaa
     7501 aggcgtcaag tctgtccgcg gtggcgcaca gggtgatttg ctgtgccgcg ttgtcgtcga
     7561 aacaccggta ggcctgaacg agaagcagaa acagttgctg caagagctgc aagaaagctt
     7621 cggtggccca accggtgagc acaacagccc gcgctcaaag agcttctttg atggtgtgaa
     7681 gaagtttttt gacgacctga cccgctaacc tccccaaaag cctgcccgtg ggcaggcttg
     7741 ggtaaaatta gggtgcgttg aagatatgcg agcacctgaa aagtggcggg gatcactccc
     7801 cgccgttgct cttactcgga ttcgtaagcc gtgaaaacag caacctccgt ctggccagtt
     7861 cggatgtgaa cctcacagag gtcttttctc gttaccagcg ccgccactac ggcggtgata
     7921 cagatgacga tcagggcgac aatcatcgcc ttatgctgct tcattgctct cttctccttg
     7981 accttacggt cagtaagagg cactctacat gtgttcagca tatagggggc ctcgggttga
     8041 tagtaaaata tcactcgggg cttttctcta tctgccgttc agctaatgcc tgagacagat
     8101 agcctcaagc acccgccgct attatatcgc tctctttaac ccattctgtt ttatcgattc
     8161 taatcctgaa gacgcctcgc ctatttttgt ggcgtaattt tttaatgatt taattattta
     8221 actttaattt atctatttat cgcaattatt gacgacaagc tggattattt ttgaaatatt
     8281 gtcctaacaa ataacgccga ctgacaagaa attaattatt acttttccta attaatccct
     8341 cagaaatcct gaccttaagc tatgattatc agggcttagg gtcacccgtt agcgctgata
     8401 tgactttccg tgt
//
LOCUS       DAJTVE010000158         8373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000158 DAJTVE010000000
VERSION     DAJTVE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..117
                     /gene="csgC"
                     /locus_tag="OO439_003619"
     CDS             <1..117
                     /gene="csgC"
                     /locus_tag="OO439_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6317888.1"
                     /translation="TKLSLNISPEDRVKIVVTVSDGQALHLSQQWPPSSEKS"
     gene            238..549
                     /locus_tag="OO439_003620"
     CDS             238..549
                     /locus_tag="OO439_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6317889.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            644..1177
                     /gene="ymdB"
                     /locus_tag="OO439_003621"
     CDS             644..1177
                     /gene="ymdB"
                     /locus_tag="OO439_003621"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6317890.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1119..2600
                     /gene="clsC"
                     /locus_tag="OO439_003622"
     CDS             1119..2600
                     /gene="clsC"
                     /locus_tag="OO439_003622"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6317891.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2608..3765)
                     /gene="mdoC"
                     /locus_tag="OO439_003623"
     CDS             complement(2608..3765)
                     /gene="mdoC"
                     /locus_tag="OO439_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6317892.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4160..5695
                     /gene="mdoG"
                     /locus_tag="OO439_003624"
     CDS             4160..5695
                     /gene="mdoG"
                     /locus_tag="OO439_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6317893.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5688..8231
                     /gene="mdoH"
                     /locus_tag="OO439_003625"
     CDS             5688..8231
                     /gene="mdoH"
                     /locus_tag="OO439_003625"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6317894.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2106 a         1989 c         2192 g         2086 t
ORIGIN      
        1 acgaagttga gtttaaatat ttccccggaa gatcgggtga aaatagttgt tactgtttct
       61 gatggacagg cacttcattt atcacaacaa tggccgccct cttcagaaaa gtcttaattt
      121 gttgaaatat cgagcataag atgaatctgg agagaatggt ctgctgcgaa tcagccaacc
      181 tgaaagtatg gataacacaa ccctcaagga tgactaatca ttgaggaaat agaataaatg
      241 ttcagacctt ttttaaactc tcttatgctc ggcagtttgt tttttccttt tattgccatc
      301 gcaggaagca ccgcgcaagg gggtgtgatc catttttatg gccaaattgt ggaaccggca
      361 tgtgacgtca gcacccagtc atcacccgta gaaatgaact gcccacagaa tggttctgtt
      421 ccgggcaaaa cctattccag caaagcgtta atgagcggta atgttaaaaa cgcgcaaata
      481 gcctcagtaa aagtgcagta tcttgataaa cagaaaaagc tggctgtaat gaacatcgaa
      541 tataactaag tttctgaaca acgcacgttg ctgagcagaa aaatgcgatt taaccaaaaa
      601 gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa cgcgtattca
      661 tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg cggctaatcc
      721 gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg gtccggccct
      781 gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg ggcatgccgt
      841 tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg ggccagtctg
      901 gcgtggtggt gaacaaaacg aagaccagct tttgcaggat gcctatctga atagcctgcg
      961 actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta ctggggttta
     1021 tggttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag aatttattac
     1081 ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa acgcccacct
     1141 ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg gcgagcgcgg
     1201 tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg gagaaaagtc
     1261 tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg ctggatgttc
     1321 agtattacat ctggcaggac gatatgtcgg gtcggttact gttttccgcc ctgttagccg
     1381 cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg cccggacttg
     1441 acgatatttt acgcttgctt gacagccatc cccgcattga agtccggctt tttaatcctt
     1501 tctcgtttcg cttgctgcgt ccgcttggtt atatcaccga cttttcccgt ctcaatcgcc
     1561 gtatgcacaa taaaagtttc actgtcgatg gcgtggttac cctggtggga ggacgaaata
     1621 ttggtgatgc ctattttggg gcaggggaag agccactttt ttcggattta gatgttatgg
     1681 caataggacc cgtggtagag gacgttgccg atgatttcgc ccgctactgg tattgcaaat
     1741 cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg gatcgcatcg
     1801 agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttacttacgc caaatggaat
     1861 ccagtccgtt tataaatcat ctggttgatg gaacgttgcc gcttatctgg gcgaaaacac
     1921 gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat tcactgttac
     1981 cgcagcgcct gtccgatatc atgggctcac ccagtgaacg catcgatatt atctcttcct
     2041 attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga aaaggggtaa
     2101 agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc catgccggat
     2161 acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa ctcaagccga
     2221 cgcgtgaaca aagtagtacg ttacacgatc gcggcataac cggtaattcc ggagccagcc
     2281 tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct ttcaatttcg
     2341 atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc gaaacgctgg
     2401 cacagttaat tgataaacgc tttattcaga gccagtatga tgcggcctgg cagctccgtc
     2461 tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag attattctca
     2521 aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg tcgatattgc
     2581 ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt tctcacgctt
     2641 aacaaccggt ttaccagaaa acaaaaactt cagtaacggg atgcgtaaat gaatttcata
     2701 cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa gccagttgga
     2761 ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt gaaccagata
     2821 gataaatagc gatgcgttaa caaagtaagt cacccgcgct gactggaagt taagcaaacg
     2881 gtggccaaag gagaagacca cattcaccat ccacagaccg aggaccatgg tgatcaccga
     2941 ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta aataagcgac
     3001 aaatgccaat gctgctgcaa gggtacagcc acgagacggc gtggtaaaca aggctttaag
     3061 atgagggaaa atgaaagcca gtgcaccgag gataaagaac ggcaaataaa atagcgtttg
     3121 catgacaata aaattgaaca tgccattact cagaatgggc ggatacacaa taaaaatcgt
     3181 tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata gttttaccat
     3241 cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc gtttaaatat
     3301 ccatacgcac agcgtcgtca tgactactaa caccagtaaa aaccacaggt gtgatattaa
     3361 ttcccaggcc aacgtattat atttgtcata caatgacagc ccaggccaac tttccgcttt
     3421 ccctttgacg tattgcagca taataaattg cggcaatgtc agtaagggga tggctgttaa
     3481 catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg gatagcgtaa
     3541 aaaaagcatg taggaaaagt agccggatat aacgaaaaat acctgcatgc ggaacgagtg
     3601 gatgaagtca ttaaaaaggg tcagccacaa tgacggttcg gcgctattca catgccatgt
     3661 atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca gccaggcgcg
     3721 gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg gttaactaat
     3781 ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc catagcgaca
     3841 ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc atcgctacca
     3901 ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aaattgctgg caaaccctac
     3961 acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt tgcaatcagg
     4021 tgcttaatcc atgagccagc gtgctgaacg ataccgggat tctgttgtcg gaatggctgg
     4081 ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg ctttactaat
     4141 tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag taatgttaac
     4201 cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc aagctcaatc
     4261 cttagccggg aaaggctatg aggcgcccaa aagcaacttg ccctccgttt tccgcgatat
     4321 gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact ggaacaatct
     4381 gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata ccccggtcaa
     4441 aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg attatttcac
     4501 tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg ccggttttaa
     4561 agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca tgctcggggc
     4621 cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc gcggcctggc
     4681 aattgatacc gccttgccat cgggggaaga atttccacgc ttcaaagagt tctggatcga
     4741 gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact cgccgcgcgc
     4801 gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg atgtgcagtc
     4861 gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa ccagtatgtt
     4921 cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt tgcacgactc
     4981 taacggtctc tctatccatg ccggtaatgg cgaatggatc tggcgtccgt tgaataaccc
     5041 gaaacattta gcggttagca gcttctcgat ggaaaacccg caaggcttcg gtctgttgca
     5101 gcgcggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc ttcgtccaag
     5161 cgcatgggtg actccgaaag gggagtgggg taaaggcagc gttgagctgg tggaaattcc
     5221 aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc agctgccgga
     5281 gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg aagacaaact
     5341 gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg atgtgaagca
     5401 gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt ttaccggcgc
     5461 agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca ttggtgataa
     5521 tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct ggcgtctggt
     5581 gatgcgtgtg aaagtgaaag atgccaagaa aaccactgaa atgcgtgctg cgctggtgaa
     5641 tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg aataagacaa
     5701 ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcggcattg ccgaagactg
     5761 atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg cgggaggatg
     5821 attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat tcacttgctg
     5881 atggacagtt aattaaagac gacgaagggc gcgatcagct gaaggcgatg ccagaagcaa
     5941 aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt ttctgggatc
     6001 gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa gaagagcagg
     6061 agagcgagca aaagtggcgt accgtcggta ccatccgccg ttacattctg ttgatcctga
     6121 cgctcgcgca aactgtcgtc gcgacctggt atatgaagac cattcttcct tatcagggtt
     6181 gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc tttatgcagc
     6241 ttctgcctta tatgctgcaa accggtatcc tgatcctctt tgcggtactg ttctgttggg
     6301 tgtccgccgg attctggacg gcgttaatgg gcttcctgca actgcttatt ggtcgcgata
     6361 aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag catcgcacgg
     6421 cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc ctgcgtgcaa
     6481 cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac attcttagtg
     6541 acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag cttatcgctg
     6601 aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg aagcgtaaaa
     6661 gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac atggtggtgc
     6721 tggatgctga ctcggtaatg accggtgatt gtttgtgcgg gctggtgcgc ctgatggaag
     6781 ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg gatacgctgt
     6841 atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca gccggtttgc
     6901 acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc cgcgtgaaac
     6961 cgtttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt gccggttcaa
     7021 tcctgtcaca tgacttcgtg gaagcggcgt tgatgcgccg tgcaggttgg ggggtctgga
     7081 ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt gatgagctaa
     7141 aacgtgaccg ccgatggtgc cacggtaacc tgatgaactt ccgtctgttc ctggtgaagg
     7201 gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat ctctccgctc
     7261 cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtacat gcgttgaccg
     7321 aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag tggcgtcctg
     7381 agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg aagttattga
     7441 gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg cgcgttacat
     7501 tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc atgctgttcc
     7561 atacggtctt cgtcgtcagc gcgttccttg gctgggaagt ggtgtggaat tcaccgcagc
     7621 gtgatgatga ctccacttcc tggggtgaag cgttcaaacg ccacggctca cagctgctgt
     7681 tagggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg ttctggctgg
     7741 caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc agccgtgcca
     7801 ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag tattcaccgc
     7861 cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc tcccttgacg
     7921 atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc gcaatggcga
     7981 ccgcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac gttgaacagg
     8041 cgctgaacga gacgccagag aagctgaatc gcgatcgtcg ccttgtgctg ctaagcgatc
     8101 cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgagaga tattcttcat
     8161 gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa ccggatgcgg
     8221 cttcgcaata aaaatgtagt tgcctgatgc gctacgctta tcaggcctac atcgttcatg
     8281 caatttattg attttgcaag cttttgtggg ttaggtaaag cgttcacgtc gcatccggca
     8341 aaaacaacga gccaataaaa ataccggcgt tat
//
LOCUS       DAJTVE010000159         8316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000159 DAJTVE010000000
VERSION     DAJTVE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            257..1915
                     /locus_tag="OO439_003626"
     CDS             257..1915
                     /locus_tag="OO439_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6317895.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1912..2868)
                     /locus_tag="OO439_003627"
     CDS             complement(1912..2868)
                     /locus_tag="OO439_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6317896.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3019..4134
                     /gene="macA"
                     /locus_tag="OO439_003628"
     CDS             3019..4134
                     /gene="macA"
                     /locus_tag="OO439_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6317897.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4131..6077
                     /gene="macB"
                     /locus_tag="OO439_003629"
     CDS             4131..6077
                     /gene="macB"
                     /locus_tag="OO439_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6317898.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6150..6374)
                     /gene="cspD"
                     /locus_tag="OO439_003630"
     CDS             complement(6150..6374)
                     /gene="cspD"
                     /locus_tag="OO439_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6317899.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6779..8017
                     /locus_tag="OO439_003631"
     CDS             6779..8017
                     /locus_tag="OO439_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6317900.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2056 a         1956 c         2230 g         2074 t
ORIGIN      
        1 tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc tttaggcgta
       61 ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga cttatcagca
      121 agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc ttaaatgtga
      181 tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg ataagtcaca
      241 tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc gcggtatcaa
      301 ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg cgtggggtaa
      361 atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc tctaccattt
      421 tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac atcatctgca
      481 tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc gttatcggcc
      541 gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt atcgtctgga
      601 aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg ataaagacgg
      661 acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc gtttaatgcc
      721 ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca cggtgccaaa
      781 tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg agttatcctc
      841 acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa tggtacagct
      901 gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt taatgcggcg
      961 gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc ggatgattga
     1021 ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta ctttgttgca
     1081 ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga tcgaagatcc
     1141 agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga atcttctgcc
     1201 attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc cggtagagca
     1261 tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg ggccgagtgg
     1321 cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc gtccgtcatc
     1381 gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg ttatcaatga
     1441 actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca ttgagtttgc
     1501 ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg aatggcatgt
     1561 actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca gcctgttgaa
     1621 taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg atatggaaca
     1681 ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa tcccggaaaa
     1741 tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt ccaaacccga
     1801 tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg taccgacgct
     1861 gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg attaaccgcg
     1921 aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc gacggtattc
     1981 agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg ctggaaggcg
     2041 gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt tgtaatcagc
     2101 gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg tttcattgct
     2161 gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg cttccatcac
     2221 gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt cctgatgtgg
     2281 aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc cctgatattc
     2341 acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa acaggattgt
     2401 gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga actgctcgcc
     2461 gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata agctgaattc
     2521 ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct ccagcaattg
     2581 cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca gtcgcggttg
     2641 gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc actcaacgct
     2701 taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac ggcgatgcca
     2761 gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca ggctgctgag
     2821 agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga aatcaatggt
     2881 tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag tgtgattact
     2941 aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg attggcatat
     3001 ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta cgttattgcg
     3061 ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc acccgtgccg
     3121 acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct ggcgaccgga
     3181 aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca gttgaaaacg
     3241 ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt cattgatcct
     3301 gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct acgcgcgcag
     3361 cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg tcagcaacgt
     3421 ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac ggagatggct
     3481 gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca ggcttctctc
     3541 gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc cggggaagtc
     3601 acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc accgaacatt
     3661 ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga agcggatgta
     3721 atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc actgacgcgc
     3781 tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga cgctattttc
     3841 tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga tatgactgcg
     3901 caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct gtcggcgtta
     3961 ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg cgaaacacgc
     4021 gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt caaagggctg
     4081 gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca atgacgcctt
     4141 tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag gttgaggtgc
     4201 tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt ggcgcttcgg
     4261 gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc accagcggca
     4321 cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg gcgcaactgc
     4381 gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat ttaaccgccg
     4441 agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag cgactgcttc
     4501 gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat tatccggcac
     4561 agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg aacggcggtc
     4621 aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc gaagaggtga
     4681 tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc acccacgatc
     4741 cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa attgtgcgca
     4801 atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt gtcaatacag
     4861 tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg gcatggcggg
     4921 cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt atcggtattg
     4981 cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg ctggcggata
     5041 ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt ggcgatgacg
     5101 atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa aaacaaccgt
     5161 gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat aacaatgttg
     5221 atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat ggcatgacct
     5281 tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag gtcgtggttc
     5341 tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt ggcgaggtga
     5401 ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa cagtcgatgt
     5461 ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc gggcgagtta
     5521 tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt gacagcgccg
     5581 aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag gatttcttta
     5641 cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact ttacaactgt
     5701 ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta atgaatatta
     5761 tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct gtaggtgcgc
     5821 gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc ctggtcggtg
     5881 gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt ttcttacccg
     5941 gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc tcgacggcca
     6001 ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat ccagtagatg
     6061 ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg gcattttgcc
     6121 tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac ttcgacgggc
     6181 acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg aacggattgt
     6241 ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc gaaaatatct
     6301 tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa ccacttaaca
     6361 gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga tggaataaac
     6421 cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa tttagagaat
     6481 tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc aaaaatttga
     6541 agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg atagatgata
     6601 gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc attcatcgct
     6661 atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg gtttgccctt
     6721 gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa cgcgaaacat
     6781 gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg gatcaccaac
     6841 cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt ccaccagcgg
     6901 cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat caccggaacg
     6961 cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa aagcggcaca
     7021 gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc gtgctgtaca
     7081 ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg aatcgtatgc
     7141 aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac acataatcag
     7201 ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg catgttttga
     7261 ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat gcaaacaggc
     7321 gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg atatggttgt
     7381 cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca aggagcttgg
     7441 ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg ccctgattcg
     7501 cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg tccaggaatg
     7561 ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga aggtagcaat
     7621 attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg agcagaacag
     7681 gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag agtacggaag
     7741 aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc ggcacacgtt
     7801 tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc taaccgcgca
     7861 ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg caaaacgtga
     7921 cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg aaaaaatcgt
     7981 tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg tttttcttgc
     8041 gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga tttatatgtg
     8101 tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg tttcttgatt
     8161 attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg tgtcatgcca
     8221 aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta aacactaacg
     8281 agcttctacg aaccttagcg aacaaagcct ttagca
//
LOCUS       DAJTVE010000160         8283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000160 DAJTVE010000000
VERSION     DAJTVE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            232..753
                     /gene="fecI"
                     /locus_tag="OO439_003632"
     CDS             232..753
                     /gene="fecI"
                     /locus_tag="OO439_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6317901.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            750..1703
                     /gene="fecR"
                     /locus_tag="OO439_003633"
     CDS             750..1703
                     /gene="fecR"
                     /locus_tag="OO439_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6317902.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1790..4114
                     /gene="fecA"
                     /locus_tag="OO439_003634"
     CDS             1790..4114
                     /gene="fecA"
                     /locus_tag="OO439_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6317903.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4159..5061
                     /gene="fecB"
                     /locus_tag="OO439_003635"
     CDS             4159..5061
                     /gene="fecB"
                     /locus_tag="OO439_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6317904.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5058..6056
                     /gene="fecC"
                     /locus_tag="OO439_003636"
     CDS             5058..6056
                     /gene="fecC"
                     /locus_tag="OO439_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6317905.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6053..7009
                     /gene="fecD"
                     /locus_tag="OO439_003637"
     CDS             6053..7009
                     /gene="fecD"
                     /locus_tag="OO439_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6317906.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7010..7777
                     /gene="fecE"
                     /locus_tag="OO439_003638"
     CDS             7010..7777
                     /gene="fecE"
                     /locus_tag="OO439_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6317907.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1799 a         2374 c         2311 g         1799 t
ORIGIN      
        1 ccctatcctt tagggcagtg aggatgtcaa taatagcaga ccacatcatg agtcattctg
       61 accgacacat gccagaagca gaggctccat cccattttat acctacctta taacacttag
      121 aaaaacaaca tgttaaaaat gtctattgga aacaatttta tttccaattg taatgataac
      181 tattctcata ttaatatggc tacgtgataa ttaacttttg atgcactccg catgtctgac
      241 cgcgccacta ccacagcttc cttaacattc gagtcgcttt atggcacaca tcacggctgg
      301 ttgaaaagct ggctgacgcg caaactccag tctgcttttg atgcagatga cattgcccag
      361 gacacttttt tgcgggtaat ggtcagcgaa acgctctcga cgatccgcga tcctcgctcc
      421 ttcctctgca ctatcgccaa acgcgtgatg gtggacctgt ttcgccgaaa cgcgctggaa
      481 aaagcgtatc tggagatgct ggcgcttatg ccggaggggg gagcgccttc acctgaggaa
      541 cgcgaaagcc aactcgagac cctacaactc ctcgacagca tgctggacgg gctaaacggc
      601 aaaacacgtg aagcgtttct gctttcgcaa ctggatggtc tgacatacag cgagattgcg
      661 cacaaactcg gtgtttccat cagctccgtg aaaaaatacg tggcgaaagc cgtcgagcac
      721 tgcctgctgt tccgtctgga gtatgggtta tgaatccttt gttaaccgat tcccgccgtc
      781 aggcgctgcg ttcagcttcc cactggtatg ccgtgctaag cggcgagcgc gtcagcccac
      841 aacaggaagc gcgctggcaa cagtggtatg aacaggatca ggataaccag tgggcctggc
      901 agcaggttga aaacctgcgc aaccagcttg gcggtgtgcc tggcgacgtt gccagccggg
      961 cgttgcacga tacccgcctc acccgccgtc acgtgatgaa aggattactg ctgttgctcg
     1021 gcgctggcgg aggctggcag ctctggcagt cggaaaccgg cgaaggtctg cgggcagatt
     1081 accgcaccgc caaaggcaca gtcagccgtc agcaactgga agatggctcc ctgctcacgc
     1141 tgaataccca aagcgcggcg gatatgcgtt ttgatgcgca tcagcgcacc gtccggctct
     1201 ggtacggtga aatcgccatt actaccgcga aagatgccct gcaacgcccc ttccgtgtcc
     1261 tgacccgtca gggccagctc actgctttag ggacagaatt taccgtccgc cagcaggata
     1321 atttcacgca gcttgacgtg cagcagcacg ccgtggaagt gcttctcgcc agtgcccccg
     1381 cgcaaaaacg catcgtgaac gctggtgaaa gcctgcagtt cagcgcctct gagtttggcg
     1441 cagtgaaacc gctggatgac gagagtacaa gctggacgaa ggacatcctg agcttcagcg
     1501 ataaaccgct gggtgaggtg atagccacgc taacccgtta ccgcaacggc gtgctgcgct
     1561 gcgatcccgc cgttgccggg ctgcgcctga gcgggacgtt cccgctgaaa aataccgatg
     1621 cgatcctgaa cgttatcgcg caaacgcttc ccgttaaaat tcagtctatt acgcggtact
     1681 ggataaacat ttcaccactg taaggaaaat aattcttatt tcgattgtcc tttttaccct
     1741 tctcgttcga ctcatagctg aacacaacaa aaatgatgat ggggaaggta tgacgccgtt
     1801 acgcgttttt cgtaaaacaa cacctttggt taacgccatt cgcctgagcc tgctgccgct
     1861 ggccggtctc tcgttttccg cttttgctgc acaggttaat atcgcaccgg gatcgctcga
     1921 taaagcgctc aatcagtatg ccgcacacag cggatttacc ctctcggttg acgccagcct
     1981 gacgcgcggc aagcagagca acggcctgca cggcgattac gacgtcgaga gcggcctgca
     2041 acaactgctg gacggcagcg gactgcaggt aaaaccgctg ggaaataaca gctggacgct
     2101 ggagcccgcg cccgcaccaa aagaagatgc cctgaccgtg gtcggcgact ggctgggtga
     2161 tgcgcgtgaa aacgacgtat ttgaacatgc tggcgcgcgt gacgtgatcc gccgtgagga
     2221 tttcgccaaa accggcgcaa ccaccatgcg tgaggtactt aaccgcatcc ctggcgtcag
     2281 cgcgccggaa aacaacggca ccggcagcca cgacctggcg atgaactttg gcatccgggg
     2341 cctgaacccg cgcctcgcca gccgctcgac cgtcctgatg gacggcatcc ccgtcccctt
     2401 cgccccttac ggtcagccgc agctttcact ggctcccgtt tcgctcggca acatgaatgc
     2461 cattgacgtg gtacgcggtg gtggtgcggt gcgttacgga ccgcagagcg tgggcggcgt
     2521 ggtgaacttt gttacccgtg ccattccgca ggactttggt atcgaggcgg gcgtggaagg
     2581 tcagctcagc ccaacctctt cacaaaacaa cccgaaagag acgcacaacc tgatggtggg
     2641 cggcacagcg gacaacggtt ttggcaccgc gctgctctac tccggcacgc gcggcagtga
     2701 ctggcgcgag cacagcgcca cccgcatcga cgacctgatg ctgaaaagca aatatgcgcc
     2761 ggatgaggtg cacaccttca acagcctgct gcaatattac gacggtgaag ccgacatgcc
     2821 cggtggcctg tctcgcgcgg attacgacgc cgatcgctgg caatccaccc gcccgtatga
     2881 ccgcttctgg ggtcgtcgca agctggcgag cctgggctac cagttccagc cagacagcca
     2941 gcataaattc aacattcagg ggttctacac ccaaaccctg cgcagcggct acctggagca
     3001 aggcaaacgc atcaccctct cgccgcgtaa ctactgggtg cgcggtattg agccacgcta
     3061 cagccagatc tttatgatcg gcccttccgc gcacgaagtg ggcgtgggct atcgctatgt
     3121 gaatgaatca acgcatgaaa tgcgttacta caccgccacc agcagcgggc agttgccgtc
     3181 cggctcaagc ccttacgacc gcgatacgcg ttccggcacc gaggcgcacg cctggtatct
     3241 ggatgacaaa atcgacatcg gcaactggac catcacgccg ggtatgcgtt tcgaacatat
     3301 cgagtcatac cagaacaacg ccatcacagg cacgcacgaa gaagtgagct ataacgcacc
     3361 gcttccggcg ttgaacgtgc tctatcacct gactgacagc tggaatcttt atgcaaacac
     3421 tgaaggctcg ttcggcaccg tacagtacag ccagattggc aaggctgtgc aaagcggcaa
     3481 tgtggaaccg gaaaaagcgc gaacctggga actcggtacc cgctacgacg acggcgcgct
     3541 gacggcggaa atggggctgt tcctgattaa ctttaacaat cagtacgact ccaaccagac
     3601 caacgacacc gtcactgcac gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg
     3661 ttacgatctg ggtacgctaa cgccaacgct tgataacgtt tccatctacg ccagctatgc
     3721 gtatgtgaac gcggaaatcc gcgagaaagg cgacacctac ggcaatctgg taccattctc
     3781 cccgaaacat aaaggcacgc tgggcgtgga ctacaagcca ggaaactgga tgttcaatct
     3841 gaacagcgat ttccagtcca gccagtttgc ggataacgcc aatacggtga aagagagcgc
     3901 cgacggcagt accggccgca ttcccggctt catgctctgg ggcgcacgcg tggtgtatga
     3961 ctttggcccg cagatggcag atctgaacct ggcattcggt gtgaaaaaca tcttcgacca
     4021 ggactacttc atccgctctt atgacgacaa caacaaaggc atctatgcag gccagccgcg
     4081 cacgctgtat atgcaggggg cgttgaagtt ctgatgtgtt aacgcccggc ttgccgggct
     4141 tttagctgga atgtgattat gttggcattt atccgttttc tttttgcagg cctgctgctg
     4201 gtgatcagcc acgcctttgc cgccacggtt caggacgaac acggcacgtt tacactcgaa
     4261 aaaacgccac aacggattgt ggtgctggaa ctctcgttcg ccgatgcgct ggccgccgtg
     4321 gacgtcagcc cgatcggtat tgccgacgat aacgatgcaa aacgcatcct gcccgaagtg
     4381 cgtgcgcacc tgaaaccgtg gcagtccgtc ggaacgcgcg cgcagccgag cctggaagcc
     4441 attgccgctc tgaaaccaga cctgatcatt gccgacagca gtcgccatgc gggggtttac
     4501 atcgccttgc agcaaatcgc gtcggtactg ctgcttaagt cccgcaacga aacctacgct
     4561 gaaaatttgc aatctgcggc tatcatcggc gaaatggtgg gtaaaaagcg agagatgcag
     4621 gcacgtctgg aacaacataa agagaggatg gcgcagtggg ccagccagct tcccaaaggg
     4681 acacgcgtgg cctttggcac atcacgcgaa cagcaattca acctgcatac tcaggagacc
     4741 tggaccggca gcgtgctggc ctctctgggg ctgaacgttc ccgctgcgat ggcgggtgcg
     4801 tccatgccgt ccatcggcct ggagcaactg ctggcggtca atcctgcctg gctgctggtt
     4861 gcccactatc gcgaagagag cattgttaaa cgctggcaac aagatccgct ctggcagatg
     4921 ttaaccgccg cgcagaagca gcaggttgct tcggtcgaca gtaacacctg ggcgcggatg
     4981 cgcggtattt ttgctgcaga gcgtattgcc gctgacacgg taaaaatctt ccaccatcag
     5041 ccgcttaccg ttgtgaaatg accgcgataa aacacccggt gctgctgtgg gggcttcccg
     5101 ttgcagcact tattattatt ttctggctga gtctgttttg ctactcggcc attcctgttt
     5161 ccggagcaga tgcaacccgc gccctactgc ctggacacac gccaacgcta ccagaagcgc
     5221 tggtgcaaaa ccttcgtttg ccacgaagcc tggtcgccgt tctgatcggc gcaagcctgg
     5281 cgctcgcggg cacgctgctg caaaccctga cccacaaccc aatggcctct ccttcactgc
     5341 tcggcattaa cagcggcgcg gcgctggcta tggcgcttac cagcgcgctg agtccgacgc
     5401 cgattgcagg ctattctctg tcgttcatcg cggcatgcgg gggcggcgtg agctggctgc
     5461 tggtcatgac cgcaggaggc ggatttcgtc atacccatga cagaaacaaa ctgatcctcg
     5521 cgggtatcgc gctgtcggcc ttttgtatgg gccttacccg catcaccctg ctgctggccg
     5581 aagatcatgc ttacggcatc ttttactggc tggctggcgg agtgtcccac gcccgctggc
     5641 aggatgtctg gcagctcttg ccggtggtgg tcactgcagt ccctgtcgtg ttgctgctgg
     5701 cgaatcaact gaacctgctc aacctcagcg acagcaccgc ccatacgctg ggagtgaacc
     5761 tgacgaggct atgtttggtc atcaatatgt tagtgctgct tctggttggt gcgtgcgtca
     5821 gcgtggcagg tccggtggcg tttatcggtc tgctggtgcc acatctggcg cgcttctggg
     5881 caggcttcga tcagcgcaac gtactgccag tgagcatgct gctgggggcc acgctgatgc
     5941 tgctggcaga tgtactcgca cgcgcgctgg ccttccccgg agatctgccc gcaggcgcag
     6001 tgctggcgct gattggcagc ccttgctttg tctggcttgt gaggaggcga ggatgaaaat
     6061 tgcgctggtt attttcatca cccttgccct ggcgggctgt gcgctgttat cactccatat
     6121 gggagtgatc cccgtgccgt ggcgcgcgct gctgaccgac tggcaggccg gacgcgagca
     6181 ttattatgta ttgatggagt accgactgcc gcgcttgctg ctggcactgt ttgtcggtgc
     6241 agccctcgcc gtggcgggcg tgctgataca ggggattgtg cgcaaccctc tggcatcacc
     6301 ggatattctc ggcgttaacc atgccgccag cctggcctct gtgggggctc tacttcttat
     6361 gccgtcactg cccgtgatgg tgctgccgct gctggccttt gcgggcggca tggcggggtt
     6421 gatattgctg aagatgctgg caaagaccca ccagccgatg aagctggcac tcaccggcgt
     6481 ggcgctttct gcatgctggg ccagcctgac ggattatctg atgctctcgc gcccgcagga
     6541 tgtgaacaac gccctgctgt ggctgaccgg cagcttatgg ggccgtgact ggagctttgt
     6601 gaagattgcc atcccgctga tgattttatt tctgccgctg agcctgagtt tttgccgcga
     6661 tctcgacctc cttgcactcg gcgatgcgcg cgccaccacg ctcggtgtgt cggtgcccta
     6721 tacccgattc tgggctttgt tactagctgt cgccatgaca tctaccggcg tggccgcctg
     6781 cggcccgatt agctttattg gtctcgtggt gccgcatatg atgcgtagca tcaccggtgg
     6841 acgtcaccgc agactgctgc ctgtttcggc cctgacaggt gcgttgctgt tggtggttgc
     6901 cgatctgctg gcgagaatta ttcatccccc actggagctc ccggttggcg tgctgaccgc
     6961 cattatcggt gcgccgtggt ttgtctggtt gcttgtgaga atgcgataaa tgactttacg
     7021 aactgaaaat ctgacggtca attacggaac agacaaggta cttaacgacg tttcactctc
     7081 actgccaacg gggaagatca ccgccctgat cggtcctaac ggttgcggga aatcgacgct
     7141 gttaaactgt ttttcgcggc ttttaatgcc gcagtctggc accgtatttc tcggcgataa
     7201 tcccataaat atgctctcat cgcgccagtt ggcccgcagg ctttcgctgc tgcctcagca
     7261 ccatttaacg ccagagggga tcacagtcca ggagctggtt tcgtatggtc gtaatccctg
     7321 gctgtcactc tgggggcgtc tctccgctga agacaatgca cgagttaatg tcgccatgaa
     7381 ccagacccgg atcaatcatc ttgccgttcg tcggttaacc gagctttccg gcggtcagcg
     7441 ccagcgcgca tttctggcaa tggtcctggc ccagaatacg cccgttgtat tacttgatga
     7501 gccaaccacc tatcttgata tcaatcacca ggtggacctg atgcggttga tgggcgaact
     7561 ccggactcag gggaaaacgg tggtcgctgt gctgcacgac cttaatcagg ctagccggta
     7621 ctgcgatcaa ctggtggtaa tggcaaacgg acatgttatg gcgcaaggca caccagaaga
     7681 ggtgatgacc ccaggattgc tgagaacagt attcagcgtg gaagcggaaa tacaccccga
     7741 gccggtatct ggcaggccga tgtgcctaat gaggtagatt gcacaggccg taagaaccaa
     7801 accacgactg aatgaaactg gactggcgcc agcaagcctg ttcagactgg ggctgaactt
     7861 ttccggactc tgaaagatta ccaatactca tcgtccatcc gcttgcttta ggctgacagg
     7921 ttcataatca acgcaaacca gagctgtaca ggcttgggcg cggctttcaa accagtcgtg
     7981 atcacggcaa tcaattttga actctgctta acggacattt ctgtataacc cttacggcaa
     8041 cgaaaaacgc gaagttaaaa ttttagaaac ccaaaaacgt gacatgacta agtttagatt
     8101 tcaggggggg gagatcaaaa aatttcgctc tgtgccagag cggacattca cggagctggt
     8161 tcattaccaa tgaggttggg cttttgagga taaatcaatg atcagacgcc aacgtaaatc
     8221 aaaagcaacc ctggaacgga attgctaatc cagtttctga ccatcgattt ttctaaaaag
     8281 tgt
//
LOCUS       DAJTVE010000161         8182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000161 DAJTVE010000000
VERSION     DAJTVE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO439_003639"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO439_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6317908.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO439_003640"
     CDS             complement(2975..3697)
                     /locus_tag="OO439_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6317909.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO439_003641"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO439_003641"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6317910.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="OO439_003642"
     CDS             6679..>8182
                     /locus_tag="OO439_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6317911.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2030 c         2321 g         1852 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAJTVE010000162         8157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000162 DAJTVE010000000
VERSION     DAJTVE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..449)
                     /locus_tag="OO439_003643"
     CDS             complement(<1..449)
                     /locus_tag="OO439_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6317912.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO439_003644"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO439_003644"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6317913.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="OO439_003645"
     CDS             complement(1788..2507)
                     /locus_tag="OO439_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6317914.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="OO439_003646"
     CDS             complement(2691..3017)
                     /locus_tag="OO439_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6317915.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO439_003647"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO439_003647"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6317916.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="OO439_003648"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="OO439_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6317917.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="OO439_003649"
     CDS             4977..5252
                     /locus_tag="OO439_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6317918.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="OO439_003650"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="OO439_003650"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6317919.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="OO439_003651"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="OO439_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6317920.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="OO439_003652"
     CDS             complement(7903..>8157)
                     /locus_tag="OO439_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6317921.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2160 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAJTVE010000163         8152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000163 DAJTVE010000000
VERSION     DAJTVE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..207
                     /locus_tag="OO439_003653"
     CDS             <1..207
                     /locus_tag="OO439_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6317922.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(249..1019)
                     /gene="yafV"
                     /locus_tag="OO439_003654"
     CDS             complement(249..1019)
                     /gene="yafV"
                     /locus_tag="OO439_003654"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6317923.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1200..1646
                     /gene="ivy"
                     /locus_tag="OO439_003655"
     CDS             1200..1646
                     /gene="ivy"
                     /locus_tag="OO439_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6317924.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1689..4133)
                     /gene="fadE"
                     /locus_tag="OO439_003656"
     CDS             complement(1689..4133)
                     /gene="fadE"
                     /locus_tag="OO439_003656"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6317925.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAM
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4373..4951
                     /gene="lpcA"
                     /locus_tag="OO439_003657"
     CDS             4373..4951
                     /gene="lpcA"
                     /locus_tag="OO439_003657"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6317926.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5157..5924
                     /locus_tag="OO439_003658"
     CDS             5157..5924
                     /locus_tag="OO439_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6317927.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5895..6635)
                     /locus_tag="OO439_003659"
     CDS             complement(5895..6635)
                     /locus_tag="OO439_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6317928.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6791..7069)
                     /gene="yafQ"
                     /locus_tag="OO439_003660"
     CDS             complement(6791..7069)
                     /gene="yafQ"
                     /locus_tag="OO439_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6317929.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7072..7332)
                     /gene="dinJ"
                     /locus_tag="OO439_003661"
     CDS             complement(7072..7332)
                     /gene="dinJ"
                     /locus_tag="OO439_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6317930.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7518..>8152
                     /locus_tag="OO439_003662"
     CDS             7518..>8152
                     /locus_tag="OO439_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6317931.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMG"
BASE COUNT         2006 a         2157 c         2048 g         1941 t
ORIGIN      
        1 tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg aaatgcagca
       61 gaattatttt cagggatacg gcacatcgct attaatattt tgacgaatga taaggtattc
      121 aaggcagggt taagacgtaa gatgcgaaaa gcagctatgg acagaaacta tctggcgtca
      181 gtccttgcgg agagcgggct ttcgtagtct taccccgact ctcccccagc cttaaacaca
      241 acccccactc accgcaacct aaactcatcc gcatcctgcc atgccggaaa cttttctcta
      301 tattcccgca atgccatcat cgacagctcc gcatcaatgc gcgttgcctg atgcgcgtcg
      361 gcagtagcga taatctcgcc ttgtggatta atcacccggc tgtcaccgcg ataatggcag
      421 ccgttgccat cgctgccgac gcgattgcat cccgccacat acgcctgatt ctcaatcgcg
      481 cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag caggccagtt ggcaacgtag
      541 atggcgaggt cataatcgtt gagattgcgc gaccacaccg gaaaacgtaa gtcgtagcac
      601 accaacggca aaatacgcca gccgcgccat tccacaatca ctcgcgcatt gcccgcttta
      661 taatgtagat gctcatctgc catgcggaac agatgacgct tatcataaaa atgtaccgtg
      721 ccgcccggct caaccagcaa aaagcggtta accgaaccag actccgtttg taatgcaaca
      781 ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg tcatccagtc cactacgtca
      841 tcttgtgcta gcgacgaagc tgccgcttcc atggcaaagc cgctggtaaa catcttcggt
      901 agaacgatca catcgcgccc ggtaatacct tccagttgac gatcaaaatg acgcaggttg
      961 gcagggccat ccatccacac cagtggttgc tgcaaaagcg taatcttcaa accaggcacg
     1021 gtgtacaact cctttatgcg aagggtttta taactttaac accttatcag gcagttgcct
     1081 tagcgcagaa taaattgata acaaatgctg atattggaaa tatctgattt gcaaattatc
     1141 gtgttatcgc caggctttag aaggttaata acatgggcag gataagctcg ggaggaatga
     1201 tgtttaagac aataacgaca gtcgccgctc tggtcatcgc caccagtgca atggcgcagg
     1261 atgatttaac cattagcagc cttgcaaagg gcgaaaccac caaagctgca tttaatcaga
     1321 tggtacaagg gcataagctg cctgcctggg tgatgaaagg cggtacttat actcccgcac
     1381 aaaccgtaac gttgggagat gatacgtatc aggtgatgag cgcgtgcaaa ccgcatgact
     1441 gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc taatcagatg acggggctgt
     1501 tctcgactat tgatgagaaa acgtcgcaag agaaactcac ctggctgaat gtgaacgatg
     1561 cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt gaccggcagc ctggaaaacc
     1621 atccggatgg ctttaatttt aaataattag cggataaaga aacggagcct ttcggctccg
     1681 ttattcaatt acgcggcttc aactttccgc actttctccg gcaactttac cggcttcgtc
     1741 gccagctctt ccggatcaaa gtcatcaacg ttaatactgc gcagacggct ttcttcagct
     1801 ttcaccagaa tagcggcttc atctttatca atcagcccct tcgccagcgc gttgtgcgcc
     1861 agttcatcca gacgggtaaa cggcaggttt ttacccagct ctttacagat ccgctgatga
     1921 attgggtcgg cggcaatcac atccaccagc gcctcttcca gcaagccaac cggattatgc
     1981 tcgctcggcg tcaggtactg accgcgacca atgcgggaac gggtggcgtt cggcacttgt
     2041 aaaatcttcg ccactttatg atccagcttg tcagaaggtg ccagataatg acgtccggtc
     2101 gggaagatca ccacattcag cagcccggca accacgcggt tcgggaagtt ttgcagtaaa
     2161 tcatccatcg cctgttcagc ctgatacagc gcatcttgta cgccccagtg caccagcggc
     2221 aggtcggctt cattacggcc ttcgtcgtca taacgcttca gcatggcaga ggcgaggtag
     2281 agctggctta aaatatcccc cagacgggcc gagatgcgct cgcgacgttt caggctgccg
     2341 tccagcactg ccatcgagac atcagaaagc agggcgaggt tggcgctcag gcggttcagg
     2401 tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa accgcgcgtc
     2461 aggcccagcc agaagctgcg aactttgttg ctaccgacgt gaccgatatg tttgaacaac
     2521 agtttatcga acgcgttgac gtcattgttc ttcgccgctt ccatctcttc cagcacgtac
     2581 ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc
     2641 ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc
     2701 ccgagcataa tgcctttgcc accagtgata tccatcgcat caataatcga ctgctgcccg
     2761 cggtgggtac agtgatactt aacgatagcc gacagcacgg caggtttttc gccgagcata
     2821 atgccgtagg taatcagcga tgccgcagca tccatcacgt aggcattacc ggcaatacgc
     2881 gccagcggct cttcaatccc ttccatctta ccgatagaga ttttgaactg acggcgaatg
     2941 tgagcatacg cgcccgttgc cagcgctacc gatttcacgc cgccggttga gttggaaggc
     3001 agggtgatgc cgcggcctac cgagaggcac tccaccagca tccgccagcc ttgcccggcc
     3061 attttcggcc caccgatgat gtaatcgatc ggtacgaaga catctttacc gcgcgtcggt
     3121 ccgttctgga acggtacgtt cagcgggaag tggcgacgac caatttccac gcccggcgtg
     3181 gtggtcggga tcagcgcaca ggtaatacct aaatcttctg cgccacccag cagcttttct
     3241 gggtcggaga gtttaaacgc cagcccaagc acagtcgcaa tcggtgccag cgtaatatag
     3301 cgtttgttcc aggtcagacg catccccagc acctgctggc cctgccattc gcccatgcag
     3361 acaatcccgg tgtccggaat cgcgcccgca tcggaacccg cttccgggct tgtcagtgca
     3421 aagcaaggga tctcctgacc acgcgccaga cgcggcagat agtgatcttt ctgctcgtca
     3481 gtgccgtaat gttgcagcag ctcgcccggg cctaatgagt tcggcacgcc gacggtaatc
     3541 gccaggatcc cgctcacgcc ggagagtttt tgcagcacgc gagactgggc ataagctgag
     3601 aactccagtc cgccgtactc ttttttgata atcatcgcga agaagcgatg ctctttaagg
     3661 tacgcccaca actccggcgg cagatccgcc agctcatgag taatctggaa atcattcgcc
     3721 atccggcagg cttcttctac cgggccgtcg agaaacgctt gctcttcggc ggtcaggcgc
     3781 ggctgcggat agttatgcag ctttttccag tccggcttgc cctggaacaa gtcgccctcc
     3841 caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc
     3901 ttacggaaac cgcgaaatac cggcgcggaa atcatcgact tacgcatagg cgcaaagtta
     3961 aatggcacga ggataatggc cagaggcacc agtacccacg ctgaccacag accagcaacg
     4021 ccgagggcgg ctgtccaggc gagcaaaatc agactgctga taaataagct cacgcggtga
     4081 tagaacaacg cgccgagcag gacaaccgta gcgagaatac tcaaaatcat cataacgaaa
     4141 agccccttac ttgtaggagg tctgaccact tgtgatgata tggttgtagt ggatgtaaaa
     4201 acatttagca atgtgtttac aatataatta caacaaagct cacattgttg ctgtttttat
     4261 ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc gcttttggcg
     4321 gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc tcatgtacca
     4381 ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact ttttaaaaga
     4441 tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca gctttaaagc
     4501 cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta tgcactttgc
     4561 cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga ttgctatttc
     4621 tgacgttagt catatttcct gcgtcggtaa tgatttcggt ttcaatgata ttttctcccg
     4681 ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca cctccggtaa
     4741 ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga aagtgatcac
     4801 cctgaccggt aaagacggcg gtaaaatggc tggcacggcg gatatcgaaa ttcgcgttcc
     4861 gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc atatcctgat
     4921 ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggccggata aggcgtttac
     4981 gccgcatccg gcacttgtgc gctgatgcct gatgcgacgc tgatgcgtct tatcatgcct
     5041 acaaatctgt acgcgaaccg taggccgaat aagacgttta cgccgcatcc ggcctgaaaa
     5101 ttcttaaatc aatcttcgcc gggggccatg cgctcccgct gttgtggagg ttacccatgt
     5161 gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt ttcaccggtc
     5221 tggtgcagcg tggtggtgga accgggccac ataaagatgg ctggggcatt accttttacg
     5281 aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc cccatcgcca
     5341 aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt cgccaggcca
     5401 atcggggcga ggtggcgctg gaaaatactc acccgtttac ccgtgagtta tgggggcgta
     5461 actggactta tgcccataac ggacaactga cgggctacaa atcactggaa accggcaact
     5521 tccgcccggt cggtgaaacc gatagcgaaa aagccttctg ctggctcctg cataaattaa
     5581 cgcagcgtta cccgcgcaca ccgggcaaca tggcggcagt gtttaaatat atcgcctcac
     5641 tggcggatga actgcggcag aagggcgttt tcaacatgct gctttctgac gggcgctatg
     5701 tgatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg tttggcgtgg
     5761 caacgctgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca ccgaatgatg
     5821 tggtcacggt gattgcaaca cagccgctga cgggcaatga aacctggcaa aagattatgc
     5881 caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag ttgtggctgc
     5941 acaacttcgt ggcttaacgg cttgctgacc acgtaacgac cattgaccac agaaacggtc
     6001 ggtggcttac gggtttgctc aaagtagtcg taacccggct tcagttgctc ccagaagtcc
     6061 ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa cgggtaaata
     6121 ctcacttgca cgctcggctg accaaacacc agcgcaccag taacgaactg gaagatctca
     6181 tcaataccct gattggtcat tgcgtagcag ccgatggaaa cacaatcgcc gtggatcatc
     6241 aggtatttcc cttcataacc atgcgcacgg tcataggcat tggggaaacc aatattaatc
     6301 gctttgtagt aacggctgtc tggttttaac tgattacgct ggacgctata aaacccttcc
     6361 gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata tttacagatt
     6421 ttataactgt cgagcagctg atattgctcg cccattttga cgtagagatc gagcgtacgt
     6481 tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt atactctttg
     6541 ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac gcacgggatc
     6601 aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg ataaaacggt
     6661 tacacacgcc aggacggcaa aatagatccc aaatcggaat agtctggatt tggaaggctc
     6721 acattatcac caaaagagtt ttacgcaagc ctgtcggcgc agggtttaca aatttcatcg
     6781 gcagcgtgct ttacccaaag agcgccgcgt gagttccagt tctctcaaat cgtaaaagtt
     6841 tatcggtaag tttgtaaatc aggatccagt ccggttcgac atgagcatcg cgataacctt
     6901 tccatgaacc ttgcagcggg tggtctttat aaacagctgg aagcggtaaa gcattattga
     6961 taagaagcgt cataagatat ttcaatttat tcatatcctt atgacgcttt tgtgcaagtt
     7021 ttacatcctt tgaaaattgt cccgagtatt caatatccct ttgaatcata tttaaattcc
     7081 taatttatca aataaatcat cagcgtcttt ggccttatga acatcaacgc cagcttcgct
     7141 gtttttgatt gattgaatgg ttaattgatt aggctcgcgt aaatcaaacg gcaatgcctt
     7201 ttcacgcgcg acctttgtga gggttatgcg aaccaggtca gagatggtca gccccatccc
     7261 ggccagtacg tccgccgcct gattcttcag atcttcatcg attcgggcgc gaacaaacgc
     7321 gttagcagcc attgggggat ctcctgtgct gtatatttat tcagcttgaa ttgtagctca
     7381 aatgagcaac agcgacaatc catgagtaat ttaataaacg gatgttttta cataataaac
     7441 tgaaatatat ttattaattt tataataatg gtttattaag tttgttcagg tacgggccag
     7501 gatacactgc aacatcaatg catgaccagc cattattgag aatgtctttg ccgtcgattc
     7561 cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca tttgcctgcg
     7621 ccaccacatc acatatctct ttcagctacg ccgcccgcca gcggatgcaa aaccgtgcgc
     7681 gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctacatt gtggaaggca
     7741 atgcccaaag cagaagggcg ctgcgccagc acaaccggga gcagataaaa cagcatccag
     7801 aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg gcggaaaaca
     7861 accacttttt aagcagcgat cacctgcata acatcaccga agtggcgatt caccgtctgg
     7921 agcagcagct tggcaagcct tacgtctggg gcggcacgcg gcccgatcaa ggcttcgatt
     7981 gtagtgggtt agttttttat gcctacaaca agatcctcga ggcgaagctc ccgcgtacgg
     8041 ccaatgagat gtatcactac cgtcgggcaa ccattgtggc gaataacgac ctgcgccggg
     8101 gagatttgct gtttttccat atccacagcc gcgagattgc cgaccatatg gg
//
LOCUS       DAJTVE010000164         8113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000164 DAJTVE010000000
VERSION     DAJTVE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..317
                     /locus_tag="OO439_003663"
                     /pseudo
     CDS             163..317
                     /locus_tag="OO439_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OO439_003664"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OO439_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6317932.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OO439_003665"
     CDS             858..2264
                     /locus_tag="OO439_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6317933.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OO439_003666"
     CDS             2509..3654
                     /locus_tag="OO439_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6317934.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OO439_003667"
     CDS             3672..4685
                     /locus_tag="OO439_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6317935.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OO439_003668"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OO439_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="OO439_003669"
     CDS             5576..6370
                     /locus_tag="OO439_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6317936.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="OO439_003670"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="OO439_003670"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6317937.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8026..>8113
                     /locus_tag="OO439_003671"
     CDS             8026..>8113
                     /locus_tag="OO439_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6317938.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCM"
BASE COUNT         1912 a         1908 c         2222 g         2071 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcacag ttgattagca taaggcttta
     7981 gagtatcgtt attaaactaa taatttataa gtagaagtat taacaatgaa agttcaattg
     8041 tacaaattca cggaagataa aaataaaacc cttaccttca gatggacgaa aaaacacttt
     8101 gagttttgta tgg
//
LOCUS       DAJTVE010000165         8103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000165 DAJTVE010000000
VERSION     DAJTVE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..777
                     /locus_tag="OO439_003672"
     CDS             <1..777
                     /locus_tag="OO439_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6317939.1"
                     /translation="KASFALQGAHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVC
                     WPWFGPAAQQGLPAHGFARNLPWTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLL
                     AHFRVGKTCEIDLESHGEFETTSALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVL
                     TDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGD
                     MPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(828..1682)
                     /gene="yeaE"
                     /locus_tag="OO439_003673"
     CDS             complement(828..1682)
                     /gene="yeaE"
                     /locus_tag="OO439_003673"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6317940.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1772..2518)
                     /gene="mipA"
                     /locus_tag="OO439_003674"
     CDS             complement(1772..2518)
                     /gene="mipA"
                     /locus_tag="OO439_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6317941.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2954..4888
                     /gene="yeaG"
                     /locus_tag="OO439_003675"
     CDS             2954..4888
                     /gene="yeaG"
                     /locus_tag="OO439_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6317942.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5001..6284
                     /locus_tag="OO439_003676"
     CDS             5001..6284
                     /locus_tag="OO439_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6317943.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6431..7906
                     /locus_tag="OO439_003677"
     CDS             6431..7906
                     /locus_tag="OO439_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6317944.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2151 a         1936 c         1882 g         2134 t
ORIGIN      
        1 aaagcctctt ttgcattaca gggcgcacac cttctctcgt ggaaacctgc gggtgaagaa
       61 gaagttctgt ggttgagcaa caacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc
      121 gtaccggttt gctggccgtg gtttggtccg gcggcacaac aaggtctgcc tgcgcacggt
      181 tttgcccgca acctgccgtg gacgctgaaa tcacataatg aagatgctga tggcgtagcg
      241 ctgacttttg aattgacgca aagcgaagag acgaaaaaat tctgtccgca cgactttacg
      301 ctgttagcgc atttccgcgt gggtaaaacc tgtgaaatcg atcttgaatc acatggcgaa
      361 tttgaaacca cctctgccct gcatacctac tttaacgtgg gtgatatcgc taaggtaagc
      421 gtcagtgggc tgggcgatcg cttcattgat aaagtgaatg acgcgaaaga agatgtactg
      481 accgacggta ttcagacctt ccctgaccgt accgatcgcg tgtatctgaa tccgcaagat
      541 tgcagcgtga ttaatgatga agcgctgaat cgtattatcg ccgtaggcca ccagcatcat
      601 ctgaacgttg taggctggaa cccgggaccg gcgctttcaa ttagcatggg cgatatgccg
      661 gatgatggct acaaaacatt tgtttgtgta gaaacggctt acgcttcaga aacgcaaaaa
      721 gtcaccaaag agaaacctgc acatctggcg caatccattc gcgttgcgaa acgttaattt
      781 gcgttaatgt tgtgtgccgg gtgcgatgca tccggcacgt atcaacatca caccatatcc
      841 agcgcagttt ttccttttgg tgccggatac gctttatcca gcatggttaa ttccgctgac
      901 gaaagttcga cctcaagcac agccgcattc tgttgaacat gggcaatcgt ggccgctttt
      961 ggaatcgcca tcacaccctg atggctgatt acccacgcca acaatacttg tgccgagctg
     1021 atattgtgag catgtgcaat ttcgttgact accgcgtttt ttaacagtcc attgcgcaac
     1081 cgcccggcct gggctaatgg actgtaagcc atcaccggca tctgctgttg ctggcaccag
     1141 gggagtagat cgtactcaat ccctcgtgaa ccgagatggt aaagcacctg attagtggca
     1201 cactgatttc cccccggcag ctgccagagt tcctgcatat cagcataatc aaggttagaa
     1261 acgccccagc ggcggatttt tccctgggcg atcaattttt ccatcgctgc aacagtctct
     1321 tcaaaagcga aactgccaga cctgtgtaat aagtagagat cgagataatc agtattgaga
     1381 cggcgtaaac tggcttcgca tgcatttatc gctttttgcc cgccagcatt ccacggatag
     1441 actttagaga cgagaaagac gttatcacgc agaccggtta atgcttcccc aaccaccttt
     1501 tcggcaccgc catcggcata catttcggcg gtatcaatga gggttaaacc gagctcaatg
     1561 cccgcgcgta gtgcagcaac ttctgttttg cgctgactgg catcttcgcc catataccat
     1621 gttccctgcc ctatggctgg cagtgagaca tcgccactaa attgaatcat tttttgttgc
     1681 attgtttcct cccaggtatt gcaccaccgt aatgcaaaac agggcgtgac gccctgtttt
     1741 tatgcacaaa atgccctgga aagatgcatt atcagaattt gtaggtgatc ccggtagaaa
     1801 tcaggccagt ccaggattta tccaccatcg ggctgtcagt aacttcatca gacagacggg
     1861 tatagcgcgc ggtaccgtaa acactccagt cgccgaggaa gttgtagctg gcgctcagct
     1921 ccaggtaagg gctccagctg tcgttcgggt tatagccacg cagaccgctg cgagcggact
     1981 ctttgcgcga tacgccataa tagtattcgt tctggttttc gctgttccac tgcacaccga
     2041 tacccggggt cactgtcagg ccgccgttgg tgtaacgata caaccaggcc atatcccaga
     2101 caatgccgtt gctgttatcc agggtatcgc cagtcagggt ggtacgcagg taaccgtact
     2161 gggtaaagtg agcataagac agaccagcca tcatggtgct cttacggtca tccaggtgac
     2221 gcatttggtg atcgccactg tctttcgctt tgaagtaaag cggcgaccaa taagcggtaa
     2281 ttgaaagttt gtccgttgcg tcattccaca ggtagtaacc accacctaag ccacggaacc
     2341 agaagttatc gccttcatag ttgattaccg gtactgggta aacatcggta tcgtaatctt
     2401 tatatgggtg ttcaacgaca ccgacgcctg cgcccaggga aaatttacct tcagcgtgcg
     2461 ctacgcctgc agacgttgcg ataagcactc caagtgccag aagtttgagt ttggtcacaa
     2521 ttaatcattc cttaaacaaa tgtttagcgg gcgacaaagt ttacccgtca atacatcgaa
     2581 acccaacctt tttacgtttt cattttttaa agtaactgtt taattttcct gacgcggatg
     2641 acaccgcgct tacagccaaa tgaatttagc gttactggcg agccttgtct ttacattaat
     2701 tatgcaaaat ttatggatga gttgttgata tgccattgaa attaagaaag ccgtgcaggc
     2761 aagttttcca tttgccatct acgcttaatt ttgaaggtgt atcaccgggc acgttgttct
     2821 catcgtcgat aaaatggcat gagagttgct gtgttttagc aagagacgtc gttcagttta
     2881 cctcttccgg gagcctctac tattcatatg aacggctctt aacctgtgct aaaaaacgaa
     2941 aggacggcat accatgaata tattcgatca ctatcgccag cgatatgaag ctgccaagga
     3001 cgaagagttc acactgcagg agtttcttac cacttgtcgg caagatcgca gtgcttatgc
     3061 caacgcggct gagcggctat tgatggctat cggtgagcct gtcatggtcg atacagccca
     3121 ggaacccaga ctttctcgac tcttttctaa ccgggtcatt gcacgttatc cggcatttga
     3181 agagttttac ggcatggaag acgcgattga acagattgtc tcttatctga aacacgcggc
     3241 tcaggggctg gaagagaaga aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa
     3301 atcatcgctt gctgagcgac tgaaatcatt aatgcagctc gtaccgattt atgtattgag
     3361 cgccaacggt gagcgtagcc cggtcaacga tcatccgttc tgtcttttca atccgcagga
     3421 agatgcgcag attctggaaa aagagtatgg cattcctcgc cgctatctcg gcaccatcat
     3481 gtcgccgtgg gcggcaaaac gcctgcatga atttggtggc gatatcacta agttccgggt
     3541 agtgaaggtc tggccgtcaa ttctgcaaca aattgctatc gccaaaacag aacccggtga
     3601 tgagaacaac caggacatct ccgcgctggt agggaaagtc gatattcgta aactcgaaca
     3661 ctacgcgcag aatgacccgg acgcctacgg ctattccggt gcgctgtgcc gcgccaatca
     3721 ggggatcatg gaatttgtcg agatgtttaa agcaccgatt aaagtgctgc atccgctgtt
     3781 aaccgctact caggaaggta actacaacgg gacggaaggt atctccgccc tgccgttcaa
     3841 cgggattatt ctcgcccact cgaacgaatc cgaatgggtc actttccgta ataacaaaaa
     3901 caacgaagcc ttcctcgacc gtgtttacat cgtgaaggtg ccgtattgct tacgcatttc
     3961 cgaagagatc aaaatctacg agaaattgct taatcacagt gaattgactc acgccccatg
     4021 cgcccctggc acgctggaaa cactgtcacg tttttccatt ctttcgcgcc tgaaagagcc
     4081 agaaaactcc agcatttatt caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac
     4141 cgatcccaaa gccaagtcgt atcaggaata tcgtgactac gccggtgtcg atgaagggat
     4201 gaaaggtctg tcgacgcgtt ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca
     4261 tgtagaagtg gcggcaaacc cggtccatct gttctacgtc ctagaacagc agatcgagcg
     4321 cgagcagttc ccgcaagagc aggcagaacg ctatctggag ttcctgaaag gttatctgat
     4381 cccgaaatat gccgagttta tcggcaaaga gatccagacg gcctaccttg aatcctattc
     4441 cgaatatggg caaaacattt tcgaccgtta tgttacctac gcggatttct ggattcagga
     4501 tcaggagtat cgcgatccgg ataccgggca gctgtttgac cgcgagtctc ttaacgccga
     4561 gctggagaaa atcgagaaac cggcggggat cagtaatcca aaagatttcc gcaacgagat
     4621 tgttaacttc gtactgcgcg ccagagcgaa taacagcgga cgcaatccga actggaccag
     4681 ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc tccaataccg aggagctgtt
     4741 gccggttatc tcgtttaacg ccaaaacgtc aaccgacgag cagaagaaac acgacgactt
     4801 tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg
     4861 gtatttgcgc gtacgtaaat cgtcttaaca accctggccc ggtacacatg ctaccgggcc
     4921 tataacgaca gcgaaccgtg ggccagagaa atggcaacac aggcgtagca tacagttggc
     4981 aaatgtagta cggggggcat atgaccgggt ttattgaccg gcgtctgaac ggcaaaaaca
     5041 aaagcatggt gaatcgccag cgttttttac gccgttataa agcgcaaatt aaacagtcga
     5101 tctccgaggc cattaataag cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca
     5161 ttcccacgga agatattagc gaaccgatgt ttcatcaggg gcgtggcggc ctgcgccacc
     5221 gcgtacatcc gggcaatgac catttcgtcc agaacgaccg aattgaacgt ccccagggtg
     5281 gcggcggagg ttccggcagt ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg
     5341 aatttgtctt tcagatttcg aaagatgagt atcttgatct gctctttgaa gatttggcct
     5401 taccgaatct gaaacaaaac caacaacgcc agctgaccga atataaaacg catcgggcgg
     5461 gttataccgc taacggcgtt ccagccaata tcagcgttgt gcgttccttg cagaactcac
     5521 tggcgcgacg cacagccatg acggcgggca agcgacggga acttcatgcg ctggaagaga
     5581 atttggccat catcagcaac agtgaacctg cgcaactgct ggaagaggaa cgtctgcgca
     5641 aagaaattgc agaattacgt gccaaaattg aacgcgtccc ttttattgac accttcgatt
     5701 tacgttacaa gaactacgag aagcggcccg atccctccag ccaggcagtg atgttttgcc
     5761 tgatggacgt ttccggttca atggatcaat ccactaaaga tatggctaag cgtttttata
     5821 ttctgctgta tctgttcctc agcagaacgt ataagaacgt ggaagtcgta tacatccgcc
     5881 atcataccca ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag gaaacaggcg
     5941 gcaccattgt ttccagcgcc ctgaaactga tggatgaggt agtgaaagag cgttataacc
     6001 cggcacagtg gaatatttac gctgcacaag catcggacgg cgataactgg gccgatgact
     6061 ctccgctttg ccatgaaatc ctggcgaaaa aattattacc tgttgttcgt tattacagct
     6121 atatcgaaat tacccgtcgt gcacatcaga cattgtggcg agaatatgag catctgcaat
     6181 ctactttcga caactttgcg atgcagcaca tccgcgacca ggatgatatt tatccggtgt
     6241 tccgtgaact gtttcataaa caaaatgcaa cagctaaaga ctaaaactat cagccaggtc
     6301 attatcgcct ggctgatttt tagcttactg taaattatct catttattac atacattagc
     6361 ttacaatcgc tttaaatatg acagcataac ctttacataa tttagttcca gaaaacaatc
     6421 attcggaaaa atgattcagt caacacgtat ttccatgggg ttattcttta aatatttttt
     6481 atcgttaacg aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa taaaatccag
     6541 cactcacatt gctctccttt ttatggtttc tatgggtaca caaaaattaa aagctcaaag
     6601 cttttttatt ttcagtttat tgctgacgtt aattttattt tgcattacta ccttatataa
     6661 cgaaaacaca aatgtaaaac tcatcccaca gatgaattac ctgatggttg ttgtggcttt
     6721 gtttttcctt aacgccgtca tttttctttt catgttaatg aaatatttca ctaacaaaca
     6781 aattttacca acactcattt taagccttgc atttttaagt ggccttatct atttagttga
     6841 aaccattgta attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa
     6901 tgatgtttct attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc
     6961 actcttttgt tatggaaaag acaacatcct tgacaacaat aagaaaaaaa cgggaatcct
     7021 gttgctggcg ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta
     7081 taacgctgac tattctttgt atgtcgtcga ctactgtccg gacaaccata ctgcgacctg
     7141 gggaatcaac tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat
     7201 catgcgtaca cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc
     7261 gctatgctgc aacttacttc tactgactct ggatgagtat aattacacca tctggtatat
     7321 cagtcgcggg attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat
     7381 ttttcagaaa ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta
     7441 taatcggcgc tactttttca acagcgtaga gtcattattg tcgagacctg ttgttaagga
     7501 cttctgtgtc atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca
     7561 tcgtgtgggt gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc
     7621 cgatgatatt ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa
     7681 tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg
     7741 ctttagtaac agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc
     7801 aacgggtgac acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga
     7861 agcgaaaagc gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc
     7921 agagccatgc tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc
     7981 cctccccagc ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac
     8041 tttatctggt tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca
     8101 tag
//
LOCUS       DAJTVE010000166         8094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000166 DAJTVE010000000
VERSION     DAJTVE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..796)
                     /locus_tag="OO439_003678"
     CDS             complement(101..796)
                     /locus_tag="OO439_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6317945.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OO439_003679"
     CDS             complement(789..2216)
                     /locus_tag="OO439_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6317946.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OO439_003680"
     CDS             complement(2227..2946)
                     /locus_tag="OO439_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6317947.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO439_003681"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO439_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6317948.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OO439_003682"
     CDS             4554..5879
                     /locus_tag="OO439_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6317949.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OO439_003683"
     CDS             5988..6224
                     /locus_tag="OO439_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6317950.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OO439_003684"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OO439_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6317951.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OO439_003685"
     CDS             complement(6989..7858)
                     /locus_tag="OO439_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6317952.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2189 a         1982 c         1728 g         2195 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga atta
//
LOCUS       DAJTVE010000167         8086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000167 DAJTVE010000000
VERSION     DAJTVE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1342
                     /gene="yjeM"
                     /locus_tag="OO439_003686"
     CDS             <1..1342
                     /gene="yjeM"
                     /locus_tag="OO439_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6317953.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1493..4816)
                     /gene="mscM"
                     /locus_tag="OO439_003687"
     CDS             complement(1493..4816)
                     /gene="mscM"
                     /locus_tag="OO439_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6317954.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4838..5806)
                     /gene="psd"
                     /locus_tag="OO439_003688"
     CDS             complement(4838..5806)
                     /gene="psd"
                     /locus_tag="OO439_003688"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6317955.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5903..6955)
                     /gene="rsgA"
                     /locus_tag="OO439_003689"
     CDS             complement(5903..6955)
                     /gene="rsgA"
                     /locus_tag="OO439_003689"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6317956.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7050..7595
                     /gene="orn"
                     /locus_tag="OO439_003690"
     CDS             7050..7595
                     /gene="orn"
                     /locus_tag="OO439_003690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6317957.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7806..7881
                     /locus_tag="OO439_003691"
     tRNA            7806..7881
                     /locus_tag="OO439_003691"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7839..7841,aa:Gly,seq:gcc)
BASE COUNT         1862 a         2092 c         2180 g         1952 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagc gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgacgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg ttctttacgc tttgctattc
      841 accgctgaaa gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg
      901 cctgaatgcg atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt
      961 cttcatcctg ctggtttcgt ttggtggcgg taccgcatcc gcgttcttta acaagctgac
     1021 gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccatt
     1081 ctttaaagca cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc
     1141 aatgattgcg atggtggttg tcgtactggt ggtgacattt gcgaacgtct tcaccattat
     1201 tcagcctgtg gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt
     1261 cttcttctcg ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa
     1321 acctgagtta gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat
     1381 gagttgtcag gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat
     1441 gatgttaagc tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac
     1501 ttcccgcctg acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga
     1561 ggctttccag acgcatctgg aagggcggga atggcatatc gataccgtgg gcatggaagc
     1621 cagccagaat cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg
     1681 cataaatacg cagctcgaaa atctgaatcc cttgttgcag atccaccagg aagacttccg
     1741 gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca
     1801 cttcttcact gttggcatca gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt
     1861 cagagagcga ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct
     1921 ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt aattttcgtc acgctaccgg
     1981 tgagatcgcg aattgtcacc gtatcgccaa tgcgaatcgg tttttcgaac aggataatca
     2041 gaccagagat aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg
     2101 cggcgaccag ccactgcaat ttcgaccact caataccaat catcgagaag ccgaccagcc
     2161 cgccaatcag catcagcaga tatttggtga tggtggtgat ggcgtagccc gtacccggcg
     2221 ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct
     2281 gcgtggtgat gataaacacc agaatggcaa tcagcaccgc accgagggta attggctcca
     2341 gactttctac gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc
     2401 cgaaagcgga atggatttct gaccacagca caatgacaga aagcagggcg atcaacatca
     2461 aaattgagcg caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt
     2521 catcgacttc aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg
     2581 cacgttgcgc taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcggc
     2641 gctggatcag catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg
     2701 caaccgaggt ttcaagcctc gctaacagcg cctgtgccgt tgccagataa cccacagccg
     2761 acgccagaat ggcaaccaac ggcgcgccaa tcatcatgtt ccacagcata tggttggtaa
     2821 tgttgtcgcc gctgccttct ttgttgagat acagcggaat cccggccttt ttcaggctga
     2881 gggtgaccac cgccagcgca ccgcaaatga ggataaagca aagccttccc agcgaaccgg
     2941 agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ggcacaataa
     3001 gcccgatgct catcaggtag tagcgcatcc cacgagatac gcgttcgcgc ggccagccaa
     3061 aatgagcgat aaacaagccg ttcgggcggg caaaggtggc gcaaatcatc actacccaca
     3121 gcagcggcac ggtggccgtt acaccatcgc caatcgcgac cgccagcgga taaggccacg
     3181 cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg taacggtgac gcgacgagaa
     3241 tcgaccagaa aagagtgcgc aacgtcagcc agaagtgatc ctgagtcact ttgccaactt
     3301 tcgccgccga acgttcaaga aaacgggtga aatagcggcg cgagtaaata ctgcaaccga
     3361 ccagaatcaa cgcgccaaac agcggcaaaa ttgtctcttt gctggtcagc atcatcacac
     3421 tggctttgcc caactgactg aaggtgtcca gcgaaatgag acgacgcaga tcctgggcga
     3481 tttccagcgg ccaggcgatg gtcatcggac gcacgtcaga ggtccagaac agatagcggt
     3541 gcgttgcttc gttcacctct ttcagcgcat cctccagttg cccgttggag actttcagct
     3601 tggtcagttc caatagtagc gtgtcgccac cctgcaacaa tgagttcagc aactcacgct
     3661 gagtgcgcag ttgtgcttcc agaatacggt tttgctcggc ggtcagcggc tgaccgtcgg
     3721 cctgatggat ttgccgtagc agcggctgtt tattaagcag atcctcataa cgtaaccgtt
     3781 gcacacgcaa ctgcgccatt tcggtatcaa gctgttgtgg ttttggcatt tctggtagcc
     3841 gtgccacctg cgctcgcaac gcttcaccga gcagattgga cgatcccagc cattgcgact
     3901 gttcacgcag cgtattcaac gcctgccgga cctgtaacgt ctggctggca gcctgacgct
     3961 gttgcgaggc aacgagatcc atccgctgcg cctgttgatt cagcgctgcc gatagttcgc
     4021 ggttaatttt gaattgcgcg acgatatctt tcggcaaatc ggcactgttt tctgccagta
     4081 attcggtact ttccagcgcc cgctccgcct caagctgacg ttggctattc aactgattac
     4141 gtaaggcctg caaatacgca tccagttgct ggctctcttt ttccgcaagc tctgagcgta
     4201 agcgcgctaa ttcttggcgg ttattggcag acagctgcgc cagctccagt tcatcaacga
     4261 gcgccttaag acgtgcagag tcagactgca acgcgaaatt ttgtgcctga ttgagcggag
     4321 tattgccggt aagcgttccc aggcggcgct cgatctcatt taactgacgg cgggcgtcgg
     4381 tttgctgttg cggcagttga ttcagcgaat cggcaatctc gcgggcgcgc tcctgctctt
     4441 gctgggcctg acggctttta tccagcaact ggctgctgac ctggagaatt tcctgattca
     4501 gcgcgtcggt agacattccc ggcgacacgc tgcgcggctt gtcacgcatg ttgtttaatt
     4561 gtgcgcgcag agtagcggag agtttcggat aattatcgat aacttgctga tattgtttga
     4621 tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa ggcagactga agcgcctcta
     4681 cgacttccgg ctgtgcgggt ttcgccgctt ttgcctgctc cagttcctga gtgatttgtt
     4741 tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag gcaccaggcc atcagaaaag
     4801 tgataatcag gcgcacgtca gcgtttcctt tgatggatta gacctggtct tttttgtcgt
     4861 caaccaatgg gctggcgtcg tgttctgctt cgatctcttc agcaggaagc ggggcaggtt
     4921 cagcgtctgg cgtaacaaag gtttcggtag atactgccag cggctggcaa attttcgtga
     4981 cagacaggct ttccagttgc tcaaccagat tcactttacc cggtgcaaac aggttgataa
     5041 cggtggaacc gagtttaaag cgacccattt cctggccttt cagcagtgcc acagaaccgt
     5101 cgttttcccc ggcaggccag gtccagcgct tgatgatacc ttcgcgcggc ggcgtaatgg
     5161 tgcccgccca gaccgtctca atgctgccaa caatcgtcgc tccgaccaga atctgcgcca
     5221 ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc gttacgggca aacagattcg
     5281 gcacgttctg agccgtgaga tggttaacgg agaagagatc gcccggcacg tagatcatct
     5341 cacgcagaat accgttgcac ggcatgtgta cgcggtggta gtcacgcggg gagaggtaag
     5401 tggtcacaaa cgtaccgttg cggaacaggt ccgccatcag atagttgcct gccagcaggg
     5461 cttcgaggct gtagttgtgg cctttggctt gcaggatttt atcttcttcg attttaccca
     5521 actggctgat aacgccatcg gcaggcatga ccagtacatt cggatcggta tcgattgggc
     5581 gtacttcgtc acgcagcgga cggacaaaga attcgttaaa ggtgcggtag ctggcggtgt
     5641 ccggcttttg cgcctctttc atgtcgacct tgtagtattt aacgaacaga tcgataacca
     5701 gttttgtcaa ccatcctgcc cgcttgcttg cgccccaacc cgccaggcga gtaagccata
     5761 gtttcggcag aatgtactgt agcgaaagtt taaatgaatt taacaaggta gcctccaggc
     5821 cattgttttg tcgttcctga ttcggcctac atgccggatc ctgaaaaaaa ggggacgatt
     5881 ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga aaagttttta cgcgttttta
     5941 cctgcgccat gctttccaga atacggtgat agttttcgaa acgggtttcc gcgattttcc
     6001 cttcctcaac cgcttcccgg atagcgcagc ccggatcggt atcgtgtttg caatcgcgat
     6061 atttacacag acctaaatag tcatggaatt cgacaaagcc ctgagtgatt tgttccggct
     6121 ccaggtgcca gaggccgaac tcacgcacac caggggaatc aatcacatca ccgccgtgcg
     6181 ggaagtgata cagccgagcg gcggtcgtgg tgtgctgacc aagccccgag ttgtcagaga
     6241 catcgttggt caggatctct ttttgcagcc ccagcaacgc gttcagcagg ctggatttgc
     6301 cgacgccaga ctgcccggca aaaatgctga tgcgcccggt caacgcctct tccagcggtt
     6361 ttagcccatc ctgagtatgg ctggaaacca tcaatacgcg ataaccgata ttgcggtaga
     6421 tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc cagcaggtct atcttgttga
     6481 gcacaataat cggctcaatc tgcaaggttt cgcaggccac taggtaacgg tcgataatat
     6541 tgagcgacag ctccggcaaa atggcggaga caatgacaat ctggtcgatg ttggcggcaa
     6601 taggttttac gccgtcgtag aaatccggac gcgtcaacac cgaggtacgc tcatgcaccg
     6661 cttccacgat ccctttgaca tttacgcctt ccgccgccgg tttacccgga cgccagacta
     6721 cgcggtcgcc ggttaccagc gaacggatgg tacggcgaat attgcagcgg tgaacgtcgc
     6781 catcggcgga ttccacatca gcgtgcatac caaagcggct gatgacgata ccttcatcag
     6841 gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt agacgtttta agacgacgct
     6901 ggtgattggc gttcacgcgg cgctgctggc ctttggagag tttattttta ctcaatcgta
     6961 ctgactcctg gtcgcccctg atgggcaaaa catctatgat acacgcaatt gtggatcaat
     7021 atagtcactg tgaatgggtg gaaaatagca tgagtgccaa tgaaaacaac ctgatttgga
     7081 tcgatcttga gatgaccggt ctggatcccg agcgcgatcg cattattgag attgccacgc
     7141 tggtgaccga tgccaatctg aatattctgg cagaagggcc gaccattgca gtacatcagt
     7201 ccgatgaaca gctggcgctg atggatgact ggaacgtgcg cacccatacc gccagcgggc
     7261 tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga agctgaactg gcaacgctcg
     7321 aattcttaaa acagtgggtg cctgcgggaa aatcgccgat ttgcggtaac agcatcggac
     7381 aggaccgtcg tttcctgttt aaatacatgc cggagctgga agcctacttc cactaccgtt
     7441 atctcgatgt cagcaccctg aaagagctgg cgcgccgctg gaagccggaa attctggatg
     7501 gttttaccaa gcaggggacg catcaggcaa tggatgatat ccgtgaatcg gtggcggagc
     7561 tggcttacta ccgcgagcat tttatcaagc tgtaaaattt ttcctggtca cgtaagcgcc
     7621 ggaatccact tgcggcggcg tgattttgac gctaaattga acgtcttgct gataatttca
     7681 gcacttgaga taaaaacgca aaaaaaactt ttttgggggg ttgcagaggg aaagatttct
     7741 cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa ttacgaaagc aaaattaagt
     7801 agtacgcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg gtcgcgagtt
     7861 cgagtctcgt ttcccgctcc aaaatttgaa aagtgctgca aagcacagac cacccaatcg
     7921 cagtatttcg aattgcgaca aggcggcaac tgaacgaatc cttgggaact tacaaaagta
     7981 tgtgactaag gtgagtgaag gcagccaagg cagtagcggt tcgaaagacg aagattaagc
     8041 gggaatagct cagttggtag agcacgacct tgccaaggtc ggggtc
//
LOCUS       DAJTVE010000168         7969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000168 DAJTVE010000000
VERSION     DAJTVE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..239
                     /locus_tag="OO439_003692"
     CDS             <1..239
                     /locus_tag="OO439_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCR6317958.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OO439_003693"
     CDS             279..1142
                     /locus_tag="OO439_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6317959.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OO439_003694"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OO439_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCR6317960.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAVLKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OO439_003695"
     CDS             2679..4037
                     /locus_tag="OO439_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6317961.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OO439_003696"
     CDS             4180..5130
                     /locus_tag="OO439_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCR6317962.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OO439_003697"
     CDS             5140..6522
                     /locus_tag="OO439_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6317963.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="OO439_003698"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="OO439_003698"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCR6317964.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2018 c         2308 g         1724 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggtgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctgt
//
LOCUS       DAJTVE010000169         7964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000169 DAJTVE010000000
VERSION     DAJTVE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            668..1621
                     /locus_tag="OO439_003699"
     CDS             668..1621
                     /locus_tag="OO439_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6317965.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1621..1917
                     /locus_tag="OO439_003700"
     CDS             1621..1917
                     /locus_tag="OO439_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317966.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2241..2531
                     /locus_tag="OO439_003701"
     CDS             2241..2531
                     /locus_tag="OO439_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317967.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2752..2967
                     /locus_tag="OO439_003702"
     CDS             2752..2967
                     /locus_tag="OO439_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317968.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3114..3419
                     /locus_tag="OO439_003703"
     CDS             3114..3419
                     /locus_tag="OO439_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317969.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3895..4605
                     /locus_tag="OO439_003704"
     CDS             3895..4605
                     /locus_tag="OO439_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6317970.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4630..4836
                     /locus_tag="OO439_003705"
     CDS             4630..4836
                     /locus_tag="OO439_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317971.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4935..7115
                     /locus_tag="OO439_003706"
     CDS             4935..7115
                     /locus_tag="OO439_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6317972.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7272..7860
                     /locus_tag="OO439_003707"
                     /pseudo
     CDS             7272..7860
                     /locus_tag="OO439_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2625 a         1421 c         1704 g         2214 t
ORIGIN      
        1 cataacgggc ttactattcg ttcaccgttt cctagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc acgaaattac acagacggtt tgctgtggtg acacgctatt accgttaacc
      121 atcattaaca gaggaggctt gagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt gcattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctagaattc tcataaatat
      301 ggtcatttac aagataggtg attcagatat taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa ttattctgtg aaataattgt tgcaatttat aatcacatct gttatagttt
      421 ttatatgggg tgataaaaga actcagaaca ttaagaggtt acaacgttgg gaggcgtcag
      481 ggtattgaca gattttcacg gcctcttatt caatcaaggc ttttcctttt gctgttaata
      541 ctcgcgtaat cgctcaggct tctgagttcc tttatcagtc catcttctaa ttaattcact
      601 ccctctgggg ttggtcacta tacgtggtca gccctttctt tgttttattt ttaaaaagga
      661 aaatataatg gaaaaattcc acatgtttga tgaccgtatt tatcagatga ctaatggcgt
      721 taaagctgcc gaccgtatta tttacactgt catgttgaat gaacaaaagt tttatcacga
      781 taaggggcaa tattataccc cttcatatgc tcatcttggt cagtatgctg gaatcacaag
      841 aacatccgtg attgcatgta tgaaacaact tgaggcacat ggtttaatca agaaaactaa
      901 cgttgcaagc aatcagtgtg ttattaaagt ttattcactt gatgaagtac cggaggtttt
      961 aacagagctt acagctaagg aagccagaaa ggcaaagctg gagtctaaag ttactcagtc
     1021 agtcaagaaa gatgacccac tgagtcaaaa ttctttaccc actgagtcaa aattttttac
     1081 tcagtcagtc aaaatctttg acacgtataa taacagtaat aacaagaaag aacttattaa
     1141 taacaataaa gaagatgact ttgaagaaga atattttaag aagggagctt cgctctgtga
     1201 ttcacatgaa tgtgattctc ttggtattga agaggctatt aaagagactg ttaaaacatc
     1261 tgacttagat gaccttgata gtgaagctgc taaagaggtc gttaaaaagg aagataaaat
     1321 ctctgatgtg attgctagta gtgaccataa aaacacacag tgccccgcca ctgatgatga
     1381 attaccggat gttgaccttg atgatgaaga agaccctgaa aaggaattga aacgtgaaca
     1441 gcgtaaagca gaaattaaag aaagattcag aaatgcgaag ctaaagaaca aggttagtga
     1501 cctcgatgac tttgatgata gtgacgatga ttttactttt acaccaaaaa agaaaactca
     1561 caccgagcca aagaaagaaa aagttaaagt cagacgtgtt tatggtggag gaatgtttta
     1621 atgaaaactt ataatggcga gagaaaatac cgtgactatt tactcagacg ttacagcatt
     1681 tctcgtgaag gttacttgat gaaagatact cacggggaag tttaccggat tcgaccgaag
     1741 aaagaaggga gggattatta tttcgtcgat gatgtgacag atttaaagat tgacgctttg
     1801 aagtttgcgg tgctgtatca ttacgattta tgggattcag tccaccagct acgattaaaa
     1861 gacggtgacc ccggtaatct gagggccacc aatattatca agggaaagtg ccggtaaggc
     1921 caccagctat ttatttactg aaatctctat ctcaacacga agaagtttaa gtcgaattgt
     1981 aagctttgtc ttttttgtaa tgactcgatt cataacgcaa aacaccaata gcacaataaa
     2041 taccgcaaag cccaacgagc taaaacgatg agattcaata attgctgtga aaaaatcatg
     2101 cattaatttt ccttttgtga aaggaaacag cacctgtatc agcccctaca accatagtat
     2161 catgtaaatg aggtactgac aatatgttaa atagatgtta agattgtagt atgagtaaga
     2221 gatttaagga gatttttaca atgactcaca cagttacgcg ccttttgcca gaggcatttg
     2281 aacgactggc agcaatcaaa gccaaatatc ccgatatgaa gcacacagcg cttgttgggg
     2341 agattttaaa agagtcggta aaccttagcc ctttcgataa cgttaagggc gtcgcagagg
     2401 gttctaaggc catcagaatc acaacgggcc aactggaact actcaaagca cagtccgcag
     2461 tagcaggttg ctcaatgatt caggtgttaa gcgctaaggt ccatcaactg gcggatagat
     2521 tggaggttta atgtgttata ggttccaata ttccacactt ggccgccacc taatcagaca
     2581 gaatttagca gatgctgaat ttgaagatgt gaccctcaaa gcaaacaaac gattaaaacc
     2641 ctctcaaatc caccagctta aacacgaatt aaagccactg gtaatactcg actataacga
     2701 ccctgaacag cttgagcagg gaattaagct tatttctgaa tggggtgctt gatgaaaact
     2761 atcacagtac aacttcagac taataaagca tttcgttatt ttgaaaactt gcttgagtta
     2821 tacgaaggat ggggaagtat ccacggcaaa gatgatatct atcttcactt gtcagcccct
     2881 aactattctc tgaagactcc ggttaagcaa agctggttaa aagactacgg tcaccagatg
     2941 gggctattag tctcagactt gagctaaaaa cagcaaaaca tatctctcag gctttaccag
     3001 acgcaccaga aacgttttta agcccctaat cagggtaact tagggaatcg ccttaagtgg
     3061 tctaaaaaag gggttaaaac gtcttagaat gcgatacagg aaggatttta aaaatgaatt
     3121 acccaatgca aattatggtt gaccaagaaa ccatgaattg gttgattgac ctgaacaggt
     3181 cgggctttaa tatctatttc atgcattgtt ttgaagcgtc aacgtatatt gacaagcata
     3241 taatcaacga aattaaactt ggtgactatg ttcacaagct ggaagtaaca gacccataca
     3301 ataagcgctt tatcattgaa gagtgcaatc gacttgattt aagccctgag caattattga
     3361 agctgaatgt attcttgacc tatcagaatg aattaaaccc ttacagcgag gtgatgtaat
     3421 gacactcgtt attctctgga tgactttagg gttatttgtt ggttgctggg taggtaatac
     3481 ggtaaataag aattgatgtt accaatctgt taaaaagctg ttgacaaata accttaagca
     3541 tgagatgatg gtaataggga agagcaactc ttcctgaatt aaactattaa tcattctaca
     3601 tcaatgatag ttattctgaa aggtgcaatg cttttcgcta catgaccttt caggttagtc
     3661 tgagaggtca tctcgaaaag tactcttgtt cattcgtttg aatccttttt gaatccttta
     3721 gatgttttaa tttcttcacc ttatgaatgg ccctttataa gaaattatct agggctattc
     3781 agcttttaaa aagcatactt ggcaaaccgc cttttaagtc tcttctaagt ttagataggc
     3841 tgtcaggtgt gctttttaaa aactgaaaca cttccgctta gaagaggaaa agcaatgaca
     3901 aatgatattt acattaatgg caaagtgatt gatagcttca gtgactggtc gccacagact
     3961 gaacatccag ctatcactat tcagcgtaaa gagcatgaca agcgtattga gcttgagcaa
     4021 tcgctaaaga tgtcaccaaa aacaaccatc tttgtgtctc ctgagcctca agagatgcct
     4081 gatatttgca agagtgaagc attacttgag cttgaaaaga aatattacca actgctgaaa
     4141 gaacagcgcc ttagattaga tgacgcttac agcaaagtta ccttgatgca atcagctatt
     4201 gagccgtcag aatttgagat tcaggatgaa ttgtcacaga aaccatttgt ctattaccaa
     4261 tacgaagata atgatggttt tggtactttt caagaaaacg tcgcagccgt gattagtagt
     4321 cttcctgctg gacatcgaat tgtgaagatg gttaaggcat caagaggtgc tggtcagttt
     4381 atttacatga ctaataagag cgatgaagaa ctcgctgaaa ttgctagaca gaacatcttg
     4441 gcatcacgta acaagcaact tgataatgtt aagttgtatc tctcaagaga gcttcaggct
     4501 atgaaagagc ttgtgaaaga atatgaagat gctaagaaga ttgctatgca ggctgatatt
     4561 gagcagctta cgaagatttc tagcaaatat gcaaaggcta tttaattaat aattaaaagg
     4621 tggtggtgaa tgattgattt aacagaacgc tatgagctta ttaaagcaat tagtgaacga
     4681 ttgaaagaac aacccaacaa accaaaacga atcaaatcat tcacctcctc taagcaaaag
     4741 aatagaatca ggaaaatagc taagtggtgt cttgataaat taccaagtga ttgtcagatt
     4801 gttggtgaag atggtaatta ttatctggtt aggtaacaag attggttggg tgaaaatcta
     4861 cgggagagat tatcgagtga tatcggtagt ctttcccttt ttcgtatatg aacacttaaa
     4921 gaggtgaaac atgaatgagc aaattcacag ttgatagttt tatcgttgaa cttagtttca
     4981 gtgaaaacgt tattaagggc ttgcaaagag ttgaaaaggc agctttgcag tcagcacaaa
     5041 gaattgaacg caatttgaat agagcgttta aggtcgatac caagcaactt gacagcaatc
     5101 tgacaagttc tttagggcag cttgaaagga agtttaataa gacctttgac aagattgagc
     5161 agagagcaag aagcacaaaa gcatttcagt ttgccaccag atttaatgac actgttaatc
     5221 ctccaaaaca atcaaggcaa ccacgaatta gcggaaacag agcaatcaca gcagcgcata
     5281 gcgtgaatat gtctaagctt ggtgatatta atccgctgat ggctaaaatg tttaaagcac
     5341 agtattacag cttaagcggt aaggctggta atattggaag tgaaaagttt aacgctgaac
     5401 ttgcaaaatt aaatcaatct cttcgtgaaa cgttgaataa actcaggtca cagacaaaga
     5461 ccgccagcat taacaataca agcgacgcat ttaataatct tgcaagcaat gcgattaaat
     5521 tatctggtgc attttactct gtgatgggtg ctcttaacgc ttacaaggcc attatgaatg
     5581 caggtcttaa acgagattcc gcacagagag ccgctaagtt tgtacttggt gataaagcat
     5641 ctgaagctga ggtatttatt cgaaatttag cagacaagac aggtcttaat atctctgaag
     5701 ggctatcaag ctacgctaag tttgcagcag gtgctcaggg ttctatgtca caggaacaga
     5761 cacaggagct atttggtaat gcaacggcta tgagtcgctt aatggggctt tctaacgatg
     5821 agctaaacgg cattttgaga gcatttgagc agatggcgag caagggcaag attcaggctg
     5881 aggaattacg tgggcagtta ggtgaccgta tggctggtgc atttaagcta ttcgctgaag
     5941 cattgggtat gactgctaca gagcttgatg cagccatgaa aaacgggaag gtactttccg
     6001 ctgatactct ccctaaagtc gctaaacaga tgggcttgat gattgataaa gcgggtggct
     6061 gggctgaagt cactaagagc actcagacag ctttaggtaa gttagcaaac aattgggatg
     6121 atacgatggt gaaaatcttc tcaggttcac aggatgagct aaacggcttc ttatccagcc
     6181 tgagtaacct gcttagtgaa atggggatga gttcttctat cgctggtgat gctatcggtg
     6241 gacttatcga catgcttaaa gctggtgtag atgatattcg cgtattcaat aacaccattg
     6301 aaggctggat tttacagact aagaagttct attacagcct tgatgacact aaacgaaaac
     6361 tacttgatga agtcggtgat ggttttatca acttcgtcaa aggtcttgct atagcgttat
     6421 ctgcaaagac gctattcagt gctactacag gggttatgaa cctgacaaga gcaatcacaa
     6481 cgttaggtac aagagctaat caggtggctg gtcaggtagc tactggtaaa ggtggtggaa
     6541 aactgaaagg tgtagctggt ggtgtaggta gtgctcttgc aattggttat gctgatgacg
     6601 gttatgaaac agcgttagca ttagcttcaa tgattccaca aatcagaggc gtcactcttg
     6661 gtttatatgc attgaagaaa gcactggatt ttatgaatca ggaagtagtc aaaaacgcta
     6721 atatgcatcc gtctggggta ggtgtgggta gtgactttaa tcccgtgtat tctggtgacc
     6781 ctaataaacc gaatatgatt aacgaaggat ggagtagaat ttttagttca atgggtgaat
     6841 tgtttaacaa tgccacaatg aatattgcaa gattcaatca tccagagtta aacaacatta
     6901 agcaagattc agcattaacg agtgcagata ttcagggtct gagggatgaa attagtgctt
     6961 tgagtaagcg tattcaggag ccagtgaaag tttcacttgg tggtcaagtt gtcgttcgac
     7021 cagatgaacc atctttcttt actttcaatc aatcagctaa tgaccagttc acgcaggata
     7081 tgctcttaag ttcaagctat ccagaggaag aatgatttaa cggtgttcta cgtgaataaa
     7141 gcaaagttaa tacccacacc aaaaattatt tttcacgtag ctcacgctta acagagaaaa
     7201 tacatatata tcaaaggctt ggttaaaaat gattaaaata cactaagcct ttgaaatcaa
     7261 aggagaattt aatgatttac actaatcagc ttcgttacag tggcaaagat ggcatctatt
     7321 tccacttaag agacaatgta gatgccttct taaccttatc agcaactgaa aacatggaat
     7381 ttgatagccc tatgcaggtg actacacaga acatgcaatt atgaaggaag cttattatta
     7441 actcgtcagg gtcaattagt agaaaacttc attgacactc tggaaaactg gcgtgaccag
     7501 aagcagatta tttctgttat ctgtaaagat ggaattaaaa ttgacgattc cattattacc
     7561 aactttaaag cctctaaaga tgttactatc tctaacggtc ttcgtatcca gctaactttt
     7621 caggaaatta actttaaagc aatcgtaggt caaactgata tttcagcagc cactggcaaa
     7681 acggctacca caaatgacgg tggtgtgacc agtaagaaga atgcaggtaa cgtgaccact
     7741 gaacgcaata acgcgcctat gctatcgtgc aaagagttaa tgaatattag cgatgtatcc
     7801 ggttacgctc cagctatcag aaatgatatt ctcgatgctc agattaaatg taataaggct
     7861 tggaagctgg actattcaga cttaacagaa gctgaaaaaa ccaacgtcac caatgctaat
     7921 agtaacgctg gtcgagtagc ttcaaagctt aaaaagtact cagt
//
LOCUS       DAJTVE010000170         7948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000170 DAJTVE010000000
VERSION     DAJTVE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(277..1308)
                     /gene="rsmC"
                     /locus_tag="OO439_003708"
     CDS             complement(277..1308)
                     /gene="rsmC"
                     /locus_tag="OO439_003708"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6317973.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1411..1824
                     /gene="holD"
                     /locus_tag="OO439_003709"
     CDS             1411..1824
                     /gene="holD"
                     /locus_tag="OO439_003709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6317974.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1793..2239
                     /gene="rimI"
                     /locus_tag="OO439_003710"
     CDS             1793..2239
                     /gene="rimI"
                     /locus_tag="OO439_003710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6317975.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2254..2931
                     /gene="yjjG"
                     /locus_tag="OO439_003711"
     CDS             2254..2931
                     /gene="yjjG"
                     /locus_tag="OO439_003711"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6317976.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3022..4611
                     /gene="prfC"
                     /locus_tag="OO439_003712"
     CDS             3022..4611
                     /gene="prfC"
                     /locus_tag="OO439_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6317977.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5004..5609
                     /gene="osmY"
                     /locus_tag="OO439_003713"
     CDS             5004..5609
                     /gene="osmY"
                     /locus_tag="OO439_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6317978.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5736..5897
                     /locus_tag="OO439_003714"
     CDS             5736..5897
                     /locus_tag="OO439_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6317979.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6019..7092
                     /locus_tag="OO439_003715"
     CDS             6019..7092
                     /locus_tag="OO439_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6317980.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7089..7871
                     /locus_tag="OO439_003716"
     CDS             7089..7871
                     /locus_tag="OO439_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6317981.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1898 a         2052 c         2153 g         1845 t
ORIGIN      
        1 caccttcgca taccatcaat tcttaaaaag aattgctacc acggaggcgc attctagtgg
       61 ttttcagctt ttcgtcaata gttaattatc gccggaggtg taattgctgg aaaaatgtcc
      121 atcaggaaac tagcgtgcag gtttggtagg catgcggggg cagatgccag atgcgacgct
      181 ggcgcgtctt atctggccta cgaagggcta acgtgcaggt tttgtaggtc ggataaggcg
      241 ttcacgccgc atccgacacg gtattcggcg agataattaa cctttcttcg cctggcgggt
      301 cataatggcg cgatacacct tgaaacgccc ggtttgcgca atcacttcat ggaagccaaa
      361 tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc gctacaattc gcagctcgcc
      421 gccgctatta agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt
      481 ttgcatccca tcgtggaacg gcgggttgga gatgatcata tcaaaacgac ctttcacctc
      541 ggaaaagacg ttgctggcaa agacttcacc ttcaacacag ttggccgcaa gtgttgcgcg
      601 gctggcttct accgctggcg cagagacatc gcacaaggtg agacgaattt tcggcgaatg
      661 gcgcgcaaag gcaactgaaa gcacccccgc gccacagccg acatccagca ctttaccttt
      721 cgtgtgcgga gttaacgtcg agagcagcaa ctggctaccg acatccagac cgtcgcggct
      781 aaacacgcca ggcagcgttt tgaccgtcag gccatcgacg ctgtattcgc cccagaattt
      841 atcggcatca aataccggct gtttttccag acgaccaaaa tagaggccac agcgacgagc
      901 gctgtcgact ttattcaacg gcgcataatc tgccagcatc tgctcggcgc tgcgcacgcc
      961 gctgcggttc tcgccaacga caaaaatatc tgtccccact ggcagcagag aaagtaaatt
     1021 catcaactgg aactgggctt ccggtttgtt cttcggccag tagtaaatca gcatatcgca
     1081 atctgcgacg tcatccaccg tggcgaccag actgaaacgg gcgttatccc ccatctggcg
     1141 gcttaatacc tgccagtggt ggaattgctg ggtatgagca cggctggccg cggtatctaa
     1201 acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc
     1261 atcactgtga cgcagcaaga cttcacttgc cggggtaaat gcagacatgg aatgctcctc
     1321 aattgatact ggcggcgatt atagccatat gttggcgcgg tatcgacgaa tttgctatat
     1381 ttgcgcccct gacaacagga gcgattcgct atgacatccc gacgagactg gcagttacag
     1441 caactgggca ttacccagtg gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc
     1501 attgcgatcc cggcacacgt ccgtctggtg atggtggcaa acgatcttcc cgccctgacc
     1561 gatcctttag tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca agtgctgcaa
     1621 ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag ctggcggttg
     1681 ggtactgacg aaccgctatc actggaaggc gctcaggtgg catcaccggc gctcaccgaa
     1741 ttacgggcaa acccaacggc acgcgccgcg ttatggcaac agatttgcac atatgaacac
     1801 gatttcttcc ctcgaaacga ctgatttacc agcggcttac cacattgaac aacgcgccca
     1861 cgcctttccg tggagtgaaa aaacctttgc cagcaaccag ggcgagcgtt atctcaactt
     1921 tcagttaacg caaaacggca aaatggcggc gtttgcgatt acgcaagtgg tgctggatga
     1981 agctacattg ttcaatattg cggtcgatcc tgactatcag cgtcagggat tgggaagggt
     2041 gctgctggaa catctgatcg acgaactgga aaaacgcggc gtggcgacac tatggctgga
     2101 agtccgtgcc tcaaatgctg ccgccattgc cctgtacgaa agtttaggct ttaacgaggc
     2161 gacgattcgc cgcaattact accccaccac ggacggtcgc gaagacgcca tcatcatggc
     2221 gttgccaatc agtatgtaag acaaggtgga ataatgaagt gggactggat tttctttgat
     2281 gccgatgaaa cgctgtttac ctttgactca ttcaccggcc tgcagcggat gtttcttgat
     2341 tacagcgtca cctttaccgc tgaagatttt caggactatc aggccgttaa caagccactg
     2401 tgggtggatt atcaaaacgg cgcgatcact tcattacagc ttcagcacgg gcgttttgag
     2461 agctgggccg aacggctgaa agttgaagca ggcttgctta acgatgcctt tattaatgcg
     2521 atggcggaaa tctgcacgcc gctgccgggc gcggtttctc tgcttaacgc cattcgtggc
     2581 aacgccaaaa tcggcatcat caccaacggc tttagcgcct tgcagcaagt gcgtctggaa
     2641 cgcacgggcc tgcgtgatta cttcgatttg ctggtgattt ccgaagaagt tggcgttgcc
     2701 aaaccgaata agaaaatttt cgattatgcg ctggaacagg cgggcaatcc tgaccgttca
     2761 cgcgtgctga tggttggcga cactgccgag tccgatattc tcggtggcat caacgccggg
     2821 cttgcgacct gctggctgaa tgcgcacaat cgcgagcaac cagaaggcat cgcgcccacc
     2881 tggaccgttt cttcgttgca cgaactggag cagctcctgt gtaaacactg attgcctccc
     2941 ccccgttgat gggtaaaata gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg
     3001 cttactcaag aagaaagaat tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc
     3061 cgcacttttg ccattatttc tcacccggat gccggtaaga ctaccatcac cgagaaggtg
     3121 ctgctgttcg gacaggccat tcagaccgcc ggtacagtaa aaggccgtgg ttccaaccag
     3181 cacgctaagt cggactggat ggagatggaa aagcagcgtg ggatctccat tactacgtct
     3241 gtgatgcagt ttccgtatca cgattgcctg gttaacctgc tcgacacccc ggggcacgaa
     3301 gacttctcgg aagataccta tcgtaccctg acggcggtgg actgctgcct gatggttatc
     3361 gacgctgcaa aaggtgttga agatcgtacc cgtaagctga tggaagttac ccgtctgcgc
     3421 gacacgccga tcctcacctt tatgaacaaa cttgaccgtg atatccgcga cccgatggaa
     3481 ctgctcgatg aagttgagaa cgagctgaaa atcggctgcg caccgatcac ctggccgatt
     3541 ggctgcggca agctgtttaa aggcgtttac cacctttata aagacgaaac ctatctctat
     3601 cagagcggta aaggccacac gattcaggaa gtccgtattg ttaaagggct gaataacccg
     3661 gatctcgacg ctgcggttgg tgaagatctg gcacagcagc tacgtgacga actggaactg
     3721 gtgaaaggcg cgtctaacga gttcgacaaa gaattgttcc ttgcgggcga aatcactccg
     3781 gtattcttcg gtactgcgct gggtaacttc ggcgttgatc atatgctgga tggcctggtg
     3841 gagtgggctc ctgcgccgat gccgcgtcag actgataccc gtaccgtaga ggcgagcgaa
     3901 gacaaattta ccggcttcgt atttaaaatt caggccaaca tggacccgaa acaccgcgac
     3961 cgcgtggcgt ttatgcgcgt agtatccggt aaatatgaaa aaggcatgaa gctgcgccag
     4021 gtgcgtactg cgaaagatgt ggttatctcc gacgcgctga cctttatggc gggcgaccgt
     4081 tcgcacgttg aagaagcgta tccgggcgat atcctcggcc tgcacaacca cggcactatt
     4141 cagatcggcg acacctttac ccagggtgag atgatgaagt tcaccggtat tccgaacttc
     4201 gcaccagaac tgttccgtcg tatccgcctg aaagatccgc tgaagcaaaa acagctgctc
     4261 aaagggctgg tacagctttc cgaagagggc gcggtgcagg tgttccgtcc gatctccaac
     4321 aacgatttga tcgttggtgc tgttggtgtg ctgcagtttg atgtggtggt atcgcgcctg
     4381 aagagcgaat acagcgttga agcagtatat gaatcagtca acgttgccac tgcccgctgg
     4441 gtagaatgtg cggacgcgaa gaaattcgaa gagttcaagc gtaagaacga aagccaactg
     4501 gcgcttgatg gcggcgataa cctcgcttac atcgctacca gcatggtcaa cctgcgcctg
     4561 gcgcaggaac gttatcccga cgttcagttc caccagaccc gcgagcatta attcttgcct
     4621 ccagggcgcg gtagccgctg cgccctgtca atttcccttc cttattagcc gcttacggaa
     4681 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
     4741 tgtgatctat atttaacaaa gtaatgacat ttctgacggc gttaaatacc gttcaatgcg
     4801 tagatatcag tatctaaagc cgtcgattgt cattctaccg atattaataa ctgattcaga
     4861 ggctgtaatg gtcgttattc atcacccatc gcttttgtga tggcgaccat tgacttctgt
     4921 agagggtgaa gtctctccct attcagcaat gcaacctcgt gttgccaggc tcaaattacg
     4981 agcaaacata caggaataaa tcgatgacta tgacaagact gaagatttcg aaaactctgc
     5041 tggctgtaat gttgacctct gccgtcgcga ccggctctgc ctacgcggaa aacaacgcac
     5101 agactaccaa tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa gtcggtaatt
     5161 tcatggatga cagcgccatc accgcgaaag tgaaggcggc gctggtggat catgacaaca
     5221 tcaagagcac cgatatctct gtcaaaaccg atcaaaaagt cgtgaccctg agcggtttcg
     5281 ttgaaagcca ggctcaggcc gaagaggcag tgaaagtggc gaaaggcgtt gaaggggtga
     5341 cctctgtcag cgacaaactg cacgttcgcg acgctaaaga aggctctgtg aagggctacg
     5401 cgggtgacac cgccaccacc agtgaaatca aagccaaact gctggcggac gatatcgtcc
     5461 cttcccgtca tgtgaaagtt gaaaccaccg acggcgtggt tcagctctcc ggtaccgtcg
     5521 attcccaggc acagagcgac cgtgctgaaa gtatcgccaa agcggtagat ggtgtgaaaa
     5581 gcgttaaaaa tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc gaactttgtc
     5641 gggaggcgcg atgtgcacca cactaaaaat atcgcattga gtgattttca cgctcatatc
     5701 aagcggtgac attaactatg gtaaaggaga cgcttatgtt tcgttggggc atcatatttc
     5761 tggttatcgc gttaatcgcc gccgcacttg ggtttggtgg tctggccggt accgctgcag
     5821 gcgcagctaa aattgtcttt gtcgtcggga ttattctgtt cctggtgagt ttgttcatgg
     5881 gccgaaaacg accctagatt tcagaccatt aagaaaattt tatccaaagc cagtccagcg
     5941 gactggcttt tgcggtttta gcgaataata aattgcgtta ctttgtcatt ctttcacaac
     6001 aaaaacagga aggcagaggt ggggcagcga atacctgtaa cgcttggtaa tattgcgccg
     6061 ttgtcgctaa ggccgttcca gcctggacga atagctctgg tgtgcgaagg cggcggacag
     6121 cgtggaattt tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt taatcctttc
     6181 gatctttatc tcggcacatc tgccggggcg cagaacctct cggcgtttat ctgcaatcag
     6241 cccggttacg cgcgcaaagt catcatgcgc tataccacaa aacgcgaatt tttcgatcca
     6301 ttgcgctttg tccgtggagg aaatcttatc gatctcgact ggctggtgga ggccaccgca
     6361 agccagatgc cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt
     6421 tatatgtgcg cctgtcgtca ggatgactac gcgcctaatt actttttacc aaccaaacaa
     6481 aactggctgg atgtgattcg cgcctccagt gcgatacctg gcttttatcg tagcggagtg
     6541 tcgctggaag gcattaacta cctggatggc gggatcagtg atgcgattcc ggttaaagag
     6601 gcggcaaggc agggcgctaa aacgttggtc gtcattcgca ctgtgccgtc acaaatgtac
     6661 tacacgccgc agtggttcaa acgcatggaa cgctggctgg gtgacagtag cctgcagccg
     6721 ctggtcaatc tggtgcagca tcatgaaacc agctatcgtg acattcagca atttattgag
     6781 aaaccaccgg gcaagctgcg gatattcgaa atttatccgc cgaagccatt acatagtatc
     6841 gcgcttggca gtcggattcc ggcgctgcgt gaagactata aacttgggcg tttatgcggt
     6901 cgttatttcc tcgccacggt tggcaagcta ttaactgaaa aagcgccgct tacccgccat
     6961 ctggtgccag tggtgacgcc ggaatcgatc gttattccgc ctgcgccagt cgccaacgat
     7021 acgctggttg ccgaagtgag cgatgctccg caggcgaacg acccgacatt taacaatgag
     7081 gatctggctt gatttgccgt tttatcgata cccactgcca ttttgatttc ccgccgttta
     7141 gtggcgatga agaggccagc ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg
     7201 ttccggcgac agaggcggaa aattttgccc gcgtgcaggc gttagcggaa aaatatcaac
     7261 cgctgtatgc cgcattgggg ttgcatccgg ggatgttgga aaaacatagc gatgtgtctc
     7321 ttgatcagct acagcaggcg ctggaaaggc gtccggcgaa ggtggtggcg gtgggggaga
     7381 tcggtctgga tctcttcggc gacgatccgc aatttgagag gcagcagtgg ttactcgacg
     7441 aacagctgaa actggcgaaa cgctacgatc tgccggtgat ccttcattcc cggcgtatac
     7501 acgacaaact ggcgatgcat cttaaacgtc acgatttacc gtgcactggc gtggttcacg
     7561 gtttttccgg cagcctgcaa caggccgagc ggtttgtaca gctgggctac aaaatcggcg
     7621 taggcggtac tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc gcaaaattac
     7681 cgctggcatc gttgttgctg gaaaccgacg cgccggatat gccgctcaac ggctttcagg
     7741 ggcagcctaa ccgaccggaa caggctgtgc gggtgtttga cgtgctttgc gagttgcgcc
     7801 cggaaccaga agatgagatt gccgaagtgt tgcttaataa tacgtatgcg ttgtttagcg
     7861 ttagtgggta gcctaccctt cgtaggcagc atccggcagt cggcgcataa tgcctgatgc
     7921 gacgcttaac gcgtcttatc atgcctac
//
LOCUS       DAJTVE010000171         7946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000171 DAJTVE010000000
VERSION     DAJTVE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..441
                     /locus_tag="OO439_003717"
     CDS             109..441
                     /locus_tag="OO439_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6317982.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            588..1949
                     /gene="yegQ"
                     /locus_tag="OO439_003718"
     CDS             588..1949
                     /gene="yegQ"
                     /locus_tag="OO439_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6317983.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2279..2596)
                     /locus_tag="OO439_003719"
     CDS             complement(2279..2596)
                     /locus_tag="OO439_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317984.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3002..3901
                     /gene="yegS"
                     /locus_tag="OO439_003720"
     CDS             3002..3901
                     /gene="yegS"
                     /locus_tag="OO439_003720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6317985.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3983..4756)
                     /locus_tag="OO439_003721"
     CDS             complement(3983..4756)
                     /locus_tag="OO439_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6317986.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OO439_003722"
     CDS             complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OO439_003722"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6317987.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5950..7305)
                     /locus_tag="OO439_003723"
     CDS             complement(5950..7305)
                     /locus_tag="OO439_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6317988.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OO439_003724"
     CDS             complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OO439_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6317989.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7624..>7946)
                     /gene="gatA"
                     /locus_tag="OO439_003725"
     CDS             complement(7624..>7946)
                     /gene="gatA"
                     /locus_tag="OO439_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6317990.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
BASE COUNT         2148 a         2037 c         1783 g         1978 t
ORIGIN      
        1 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cggtaaacag
       61 cacctttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat ggctggttgg
      121 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
      181 gagactatcc tcaccagcga gctgtataca tcaaaagcct ctgcggaaaa gggcatcgcg
      241 tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc aagtaacggc
      301 aaattctatt tcaatctgaa agccgccaat catcaaatta tcggctccag ccagatgtac
      361 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
      421 acggtgaaag acaatacgta attacgatgc cgggcgtggg gtgtcggcgt ccggcgcttt
      481 accagatccc cccacagcgc tacaatgccc gcccttaaag tgggggcact cccctaaccg
      541 cttcatcagg tgaagcggag ctgacctgtc atcagaacga gagaattatg tttaaaccgg
      601 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
      661 atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac gaattcaacc
      721 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
      781 tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
      841 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
      901 gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtaaactggg
      961 cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt
     1021 cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatt gagatcttcg
     1081 ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
     1141 agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac aacgtccagg
     1201 aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
     1261 atgttgagcc gacgctaggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag
     1321 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
     1381 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
     1441 cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc gcacaggttt
     1501 atcgtaaagc tatcgatgat gccgctgcgg gcaaaccgtt cgataccagc ctgctggaaa
     1561 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
     1621 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
     1681 agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg
     1741 ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca
     1801 tggaaaacgc caaaggtgaa gcgatgccgg tcgcaccagg cgatggttat actgtgtggc
     1861 tcccggtccc gcaggatctt gagctaaatt acgcgctgct gatgcgtaat ttctccgggg
     1921 aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
     1981 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
     2041 aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
     2101 cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct tccattgacc
     2161 acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat cgtgctcgca
     2221 caggttccgg ctaagcacaa ccagaacgcg catgtttgat gcttaccaaa aaatattctc
     2281 actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct tctgtcttca
     2341 ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca tcatttttta
     2401 ctatattttc tgcaccagat attcttcccc tagctaaaga agcttcggct tcggtgtagt
     2461 attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat ttaccgatac
     2521 ttgtttcatc attatgcaca gcacaaccag atataagaaa aatactaatt aatgatatag
     2581 cagctatctt tttcatctca ccttccccca ttaaatacca acgacactct ctagtgttta
     2641 aatataataa tggcatgatt attataattg aataggatta taataaatgt tctgtacaac
     2701 atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact ccatgggggc
     2761 tttaatatat tatattgaag atgccactgt ttagttgaat attaggtata tgctcttttt
     2821 tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct cctggtggta
     2881 accactgaat cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga
     2941 ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg cacatacgat
     3001 catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc
     3061 cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac
     3121 ctgggagaaa ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac
     3181 ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg
     3241 tgagggggat ggcatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc
     3301 caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa
     3361 cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac
     3421 aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg
     3481 tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg
     3541 tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa
     3601 cgggcgtcag gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt
     3661 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
     3721 atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc
     3781 cccacacgaa atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat
     3841 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtctat tattgcgtta
     3901 atcaaactat tctctcttga accagggaga atccgaaatg ccggatgcgg tgtaaatacc
     3961 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
     4021 ccagtttatc cacctctgat acagcatcat cggtaataat aatgtcaatt ttttcgattg
     4081 gcagtacctg attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt
     4141 tattagcggc ggtgaccatc gcaccgctaa tcgaataacc ttcattaaag gtcgtaatac
     4201 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
     4261 atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac
     4321 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg
     4381 taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt gtcgtacttc
     4441 cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc
     4501 tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
     4561 tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
     4621 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
     4681 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
     4741 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
     4801 gaccattggt gaataatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
     4861 ttcatggaat gagcaacact ttgcccggca tggcattacg agcgatgtca cgcaccgcct
     4921 gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
     4981 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gaatagttca
     5041 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
     5101 aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct
     5161 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc
     5221 gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
     5281 gcatcgcacc gaaagatttt gccaatgcca gtttttctga gctaatgtcg atcgccgtca
     5341 cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac
     5401 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa
     5461 cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga
     5521 cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc gagccaataa
     5581 aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggataa gtaaaacagg
     5641 gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca
     5701 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
     5761 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
     5821 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
     5881 tatcattcac cattgatttc ataaaaactc ctgattgtta agggggataa cctccccctc
     5941 aggtaaaaat tattctgcga gaacggtttt ctcttgttta ataaaaccac gcgctctacg
     6001 ccaggtcatg aaaataccgg taagataaat tgcgccgata ataatgaaac cgggaatatt
     6061 ttgcgtggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
     6121 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
     6181 aatagtttgc gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt
     6241 gcggaacaga tttccacgat gcaccgcgac cgccatcgcc acgaagaagc cgatggtggc
     6301 aagatcgcca aacggcagta cctgattacc cggaacacaa acagcaatta aaatggtgag
     6361 cgggataaaa atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc
     6421 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
     6481 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatca gcagcataac
     6541 tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaaccgg cgaggatgcc
     6601 gataatcagc cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg
     6661 ctgaatatcg tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc
     6721 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc
     6781 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
     6841 cgccgcgtga attaccacac ctgccatccc tatcatccat gaaccggttg ccagatgcag
     6901 caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt
     6961 catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg ccaccagcgc
     7021 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
     7081 attcaggtcg aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa
     7141 gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca
     7201 atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg gaagcatcac
     7261 cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
     7321 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc
     7381 gtgaactaac ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg
     7441 cacaccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
     7501 actatgattc tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
     7561 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
     7621 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg
     7681 aagagtgatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
     7741 aagtttcaat tgctgctgcg gattctccac aatcaacgca ataaccaacg atacctccac
     7801 gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat
     7861 ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag
     7921 cattatcccg gtagggaatt ctgctt
//
LOCUS       DAJTVE010000172         7821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000172 DAJTVE010000000
VERSION     DAJTVE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(208..1098)
                     /gene="dgcZ"
                     /locus_tag="OO439_003726"
     CDS             complement(208..1098)
                     /gene="dgcZ"
                     /locus_tag="OO439_003726"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6317991.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1353..1745)
                     /gene="ydeI"
                     /locus_tag="OO439_003727"
     CDS             complement(1353..1745)
                     /gene="ydeI"
                     /locus_tag="OO439_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6317992.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2021..2539
                     /locus_tag="OO439_003728"
     CDS             2021..2539
                     /locus_tag="OO439_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6317993.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2584..4629)
                     /gene="dcp"
                     /locus_tag="OO439_003729"
     CDS             complement(2584..4629)
                     /gene="dcp"
                     /locus_tag="OO439_003729"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6317994.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4766..5512
                     /gene="ydfG"
                     /locus_tag="OO439_003730"
     CDS             4766..5512
                     /gene="ydfG"
                     /locus_tag="OO439_003730"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6317995.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5601..6287
                     /gene="ydfH"
                     /locus_tag="OO439_003731"
     CDS             5601..6287
                     /gene="ydfH"
                     /locus_tag="OO439_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6317996.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6464..6667
                     /gene="ydfZ"
                     /locus_tag="OO439_003732"
     CDS             6464..6667
                     /gene="ydfZ"
                     /locus_tag="OO439_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6317997.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6703..>7821)
                     /locus_tag="OO439_003733"
     CDS             complement(6703..>7821)
                     /locus_tag="OO439_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6317998.1"
                     /translation="PQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTA
                     TGVIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVT
                     FPSTMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAG
                     AELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQ
                     APTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDS
                     KFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKA
                     IFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2057 a         1960 c         1793 g         2011 t
ORIGIN      
        1 aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac
       61 ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt
      121 gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac agtagcatca
      181 gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt gttcgtcaat
      241 aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt ctgctcttcc
      301 aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg tcacggtaat
      361 gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc aaattctgac
      421 acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa attcttcgcc
      481 cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag ttgccagggt
      541 gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt taaatcggtc
      601 aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact gatgatcaaa
      661 ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat tgctacggat
      721 cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca acccctcctg
      781 aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca ttaattcccg
      841 accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag gtaattcatc
      901 gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc cataagaatg
      961 gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa acatactcac
     1021 cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg catcaatttc
     1081 cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg ctattctaac
     1141 gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg agtcatctct
     1201 gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa ttctgtaaat
     1261 cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata ccctaatggc
     1321 ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt gacccgaacg
     1381 acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg gtccggctga
     1441 acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc gctcttatcg
     1501 cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag tgaaaccgaa
     1561 gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc atcggttccg
     1621 cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt taaaccgccc
     1681 tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat ggactgaaat
     1741 ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg ctatttttat
     1801 aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc ccactagctg
     1861 tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc ttaaacatgt
     1921 tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc atacagaaaa
     1981 agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta ataaagataa
     2041 aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt tgagtcaaag
     2101 acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg gtaagttggc
     2161 tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct ttgttacttt
     2221 caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg aacgatattc
     2281 tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc gtgcggatgc
     2341 tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag atggtacgcc
     2401 agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg cggttatgca
     2461 ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc tcgcccagct
     2521 gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag ccattggtgg
     2581 tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag ccttaccgcg
     2641 ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga tcgcctcgcg
     2701 aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact ggtaaccatc
     2761 atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc cgccaccaaa
     2821 aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat ccatattttc
     2881 cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt tttcttccag
     2941 gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct catacccttt
     3001 gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg ctgccccgct
     3061 ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt gttcgttgat
     3121 ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg tggcataacg
     3181 ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg ttattacatc
     3241 atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc agacgttata
     3301 aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca tccatgcacc
     3361 gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc ccacgccatt
     3421 atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat caaaacgttc
     3481 gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat tcaacaccgt
     3541 gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct cccgccgtac
     3601 ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct gctgcttatc
     3661 gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg ctggaacaat
     3721 ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg cgattttcca
     3781 tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct ctaccagacg
     3841 ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc agcccgcaat
     3901 aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt gttgggtggt
     3961 attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg cctctgccgc
     4021 cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca caaccagacc
     4081 gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg tcgcagcttc
     4141 tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc cggcaaggac
     4201 aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc ccagggattc
     4261 acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat agatatcatt
     4321 agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt catcattggt
     4381 atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt ctccgctttg
     4441 ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg cgatggcagc
     4501 aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt gatgattggc
     4561 aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga aaggattcat
     4621 tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta ctgataacgg
     4681 agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat gttattgaca
     4741 cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa cggcaggttt
     4801 tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg ccactggccg
     4861 tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt atatcgccca
     4921 actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc ctgccgagtg
     4981 gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg agcctgcgca
     5041 taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag gcctggtata
     5101 tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata ttattaacat
     5161 tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg cgacgaaagc
     5221 gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg cggtgcgcgt
     5281 caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc gctttaaagg
     5341 cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc cagaagatgt
     5401 cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca ataccctgga
     5461 aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt aatttttata
     5521 cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca tgcagttaac
     5581 tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca cagcctgtca
     5641 atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt gcaccaggca
     5701 caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag ccggttcgtg
     5761 aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag cgtggcagct
     5821 acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt caggccattg
     5881 agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc taccaactgg
     5941 aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat gatttttttg
     6001 aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa ctggcgtggg
     6061 ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt ttcgaccacg
     6121 tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc ctgcaaaaac
     6181 gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc agcgaatccg
     6241 tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc atttcctctc
     6301 atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt atcaaaaaag
     6361 atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta tattgcgccc
     6421 gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga cctacgatcg
     6481 taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg gtcacactgg
     6541 caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc gcggaaaaac
     6601 cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc gtctcggcat
     6661 gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca cggaatattt
     6721 cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg tcacttttgc
     6781 agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg ccagcaatga
     6841 ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa caggtaacag
     6901 tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat aacgcatcca
     6961 acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat gccagcgaac
     7021 agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct gccaggttcg
     7081 atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat cttgcaaatc
     7141 aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat acgccgcata
     7201 acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa gataacccaa
     7261 atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt cataaggcag
     7321 cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg caacaaagtt
     7381 atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat cgcccacacc
     7441 ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa tacggtccac
     7501 cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta catcaacagc
     7561 tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca tgttgtcaca
     7621 tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg cctcaacaat
     7681 cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga ccatcgggtg
     7741 atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg tgattgtcag
     7801 agagacaatc gcgatttgcg g
//
LOCUS       DAJTVE010000173         7708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000173 DAJTVE010000000
VERSION     DAJTVE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO439_003734"
     CDS             complement(<1..304)
                     /locus_tag="OO439_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6317999.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="OO439_003735"
     CDS             complement(1125..1337)
                     /locus_tag="OO439_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6318000.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="OO439_003736"
     CDS             complement(1413..2030)
                     /locus_tag="OO439_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6318001.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="OO439_003737"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="OO439_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6318002.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="OO439_003738"
     CDS             complement(3911..4972)
                     /locus_tag="OO439_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6318003.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /locus_tag="OO439_003739"
     CDS             5214..7316
                     /locus_tag="OO439_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6318004.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="OO439_003740"
     CDS             complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="OO439_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6318005.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1964 a         1801 c         1856 g         2087 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agacccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtgtgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgccta gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 ccccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtgag
//
LOCUS       DAJTVE010000174         7650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000174 DAJTVE010000000
VERSION     DAJTVE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(120..2498)
                     /gene="ppsA"
                     /locus_tag="OO439_003741"
     CDS             complement(120..2498)
                     /gene="ppsA"
                     /locus_tag="OO439_003741"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6318006.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            2831..3664
                     /gene="ppsR"
                     /locus_tag="OO439_003742"
     CDS             2831..3664
                     /gene="ppsR"
                     /locus_tag="OO439_003742"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6318007.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3821..4867
                     /gene="aroH"
                     /locus_tag="OO439_003743"
     CDS             3821..4867
                     /gene="aroH"
                     /locus_tag="OO439_003743"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6318008.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4999..5190
                     /gene="hemP"
                     /locus_tag="OO439_003744"
     CDS             4999..5190
                     /gene="hemP"
                     /locus_tag="OO439_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6318009.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5194..6630)
                     /locus_tag="OO439_003745"
     CDS             complement(5194..6630)
                     /locus_tag="OO439_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6318010.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6693..7406)
                     /gene="ydiV"
                     /locus_tag="OO439_003746"
     CDS             complement(6693..7406)
                     /gene="ydiV"
                     /locus_tag="OO439_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6318011.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1941 a         2027 c         1817 g         1865 t
ORIGIN      
        1 tttttgttaa gaaaagatat tatgcggcgg ttaactcagg atgtctgtga agagattgaa
       61 taagtttcat cttcggggat cacataaccc cggcgactga acgccgccgg ggatttattt
      121 tatttcttca gttcagccaa gcttaaccag gtttgcacca cggtgtccgg gttcagagac
      181 aggctatcga tcccctcttc catcaaccat gcggcaaagt cttcgtggtc ggacggaccc
      241 tgaccgcaaa tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc
      301 agtgctttca ccgcatcgtt gcgctcatcg aacaattcag acaccacgcc ggagtcacgg
      361 tccagaccga gcgccagctg cgtcatatcg tttgagccaa ttgagaagcc gtcgaaatat
      421 tcgaggaact gctcggccag caaggcgttg gacgggattt cacacatcat gatgattttc
      481 agcccgttct caccacgctt cagcccctga cgcgccagtt cctcaaccac cgctttcgcc
      541 tgatctacgg tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg
      601 cgcacacgtt tcactgcttc gcactccagc gcgaagcagt cgcggaagct gtcggaaaca
      661 tagcgtcccg cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca
      721 ccaccgacca ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc
      781 ttcggataaa acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat
      841 tcacgcggag aatcaaaacc tttcatcatc tcgcggattt cattttgcaa ctgcggttcc
      901 tgatcgtcaa actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat
      961 tccagacgcg caaggcccac gccttcgttc ggcaggcagg cgaagtcgaa agcacggtcc
     1021 gggttaccga cgttcatcat cactttcaac ggcagatccg gcatcatttc tacgctggag
     1081 cttttcacgc taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa
     1141 gaaacagtga cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccactacc
     1201 gccggaatgc ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg
     1261 gtgacgatgg cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg
     1321 accagcacgt caccaggttc gatgcggttc atttcgctga tctcatggat gactttcacc
     1381 ggacccgcgc cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa
     1441 tgcagcgtat aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc
     1501 acaatgaaca gtttgccagt gtggccatct ttcgcccact caatatccat cgggcgaccg
     1561 tagtgtttct caatttgtac ggcctgtttt gccagttcct gcacttcttc gttggtcagc
     1621 gagaagatgt cacgctgttc ctgcggtacg tcttcgattt taacctgctt gccgtgctcc
     1681 tgggtcggcg cgtaaaccat gcggattttt ttcgacccca tggtgcgacg cacgatagcc
     1741 gggcgattcg ccgccagtgt cggtttatgg acgtaaaact catccgggtt aaccgcaccc
     1801 tgcacgacca tttccccaag gccccatgcg gaagtgataa acaccacctg gtcaaagccg
     1861 gattcggtat caatggagaa catcacgcca gaagatgcga ggtcggagcg caccatccgc
     1921 tgaacaccgg cagagagcgc cacgccacgg tgatcgtaac cctggtgcac acgataagag
     1981 atggcgcgat cgttaaacag agaagcaaat acatgcttca ctgccacgag aacggcgtca
     2041 aaaccctgaa cgttgaggaa ggtttcctgc tgaccggcaa aagaagcgtc cggcatatct
     2101 tctgcggtgg cggaggagcg caccgcaaaa gaggcgtttt cgtcatcggc ggaaagctgt
     2161 gcataggctt cgcggatggc gttttccagc tcaggctgga agggagtgtc gataatccac
     2221 tggcggattt gcgcgcccgc tttcgcaagc tgagtaacat cgtcaatatc cgttttatcc
     2281 agcagttcat aaatgcgctg gtttacgccg ctttggtcca gaaactggtt aaacgcgtcg
     2341 gcggttgtgg cgaaaccatt cggaacggaa acacccattc cggaaaggtt agtaatcatt
     2401 tcacccaggg aggcattttt gcccccaacc ctgtctacat cattcatgcc gagttggtta
     2461 taccaaagca ccagcggtga cgagccattg ttggacatcg aacaatcctt ttgtgataaa
     2521 tgaacggttt gagaaacaca tttctgcgca tttatctttg catatttaac cggatgaaaa
     2581 aaacggtgaa tcgttcaagc aaatatattt ttttactttt taagactgat cccagcgttg
     2641 cgcaaatctg ctctcttcga caatttccac ataaaccatc ggtataaacg aaaccataaa
     2701 aatgaaatgc tgttttcata aaaaataaaa ttgaaggttc attttataaa ccagacataa
     2761 cgtcctacgc ttctgtgcgt ttttaattta tgctttcata gaattatgtc tgcatcacgg
     2821 gaagaacaaa atggataatg ctgttgatcg ccacgttttt tatatttctg atggtacggc
     2881 aataactgcg gaggtattag gacacgcagt aatgtcacaa tttcccgtca ctatcagcag
     2941 cattacgctg ccgtttgtcg aaaatgagag ccgtgcacgg gcagtgaagg atcagattga
     3001 cgcaatttat caccagacag gtgtgcgccc gctggtcttc tactccatcg tgttgccgga
     3061 gattcgcgcc atcatcttgc aaagtgaagg cttttgccag gatatcgttc aggcgctggt
     3121 tgccccgcta caacaagaga tgaaactgga tccaacgccg attgctcatc gtacccatgg
     3181 ccttaaccct aataatctca ataaatatga tgcgcgcatt gcggcgattg attacaccct
     3241 cgcccacgat gacggcattt cgttgcgcaa tctggatcag gctcaggtga tcctgctcgg
     3301 tgtttctcgc tgtggtaaaa cccccaccag tctttatctg gcaatgcaat ttggtatccg
     3361 cgcggcaaac taccccttta ttgccgacga tatggataat ctggtgctac ccgcgtcgct
     3421 caaaccgctt cagcataaat tgttcggcct gactatcgac ccggaacgtc tggcggcgat
     3481 tcgcgaggaa cgtcgggaga acagtcgcta tgcctcgctt cgtcagtgca ggatggaagt
     3541 cgcggaagtg gaagcgttgt accgtaaaaa tcagatcccg tggattaaca gtaccaatta
     3601 ttcggtagaa gagattgcca ccaagatcct cgatatcatg ggccttagtc gccgaatgta
     3661 ctagagaact agtgcattag cttatttttt tgttatgatg ctaaccaccc ggcgaggtgt
     3721 gacacacctc gcacttgaaa tcagcagcga ttggtttatc gtgatgcgca tcacttcccg
     3781 gcagtcctgc cgtagaagca acaaatttct gagacttgta atgaacagaa ctgacgaact
     3841 ccgtactgcg cgtattgaga gcctggtaac gcccgccgaa ctcgcgctac ggtatcccgt
     3901 aacgcctggc gccgccaccc atgtcaccga ctcccgccgc agaattgaaa aaatactcaa
     3961 tggtgaagat aagcgactgt tggtcattat tggcccctgc tcgatccacg atctcaccgc
     4021 tgcaatggag tacgcccccc gtctgcagtc gctgcgcaac cagtaccagt cacggctgga
     4081 aatcgtaatg cgcacctatt ttgaaaaacc acgaactgtt gtcggctgga aaggactaat
     4141 ctccgatcca gatttaaacg gcagctatcg ggtaaatcac ggtctggagc tggcgcgcaa
     4201 attactttta caggtaaatg agctgggcgt cccaaccgcg actgagttcc tcgatatggt
     4261 gaccggtcag tttattgctg atttaatcag ttggggcgcg attggcgcac gtactaccga
     4321 aagtcagatc caccgtgaaa tggcttcggc actctcctgt ccggtaggtt ttaaaaatgg
     4381 taccgatggc aatacgcgga ttgctgtgga tgctatccgc gcagcccgcg ccagccatat
     4441 gttcctctcg ccagacaaaa atggtcagat gaccatctat cagaccagcg gcaacccgta
     4501 tggccacatt attatgcgtg gcggcaaaaa accgaattat catgccgatg atatcgccgc
     4561 agcctgcgat acgctgcacg agtttgattt acctgaacat ctggtggtgg atttcagcca
     4621 cggtaactgc cagaagcagc accgtcgcca gttagaagtt tgtgaggata tttgtcagca
     4681 aatccgcaat ggctctacgg cgattgctgg aattatggcg gaaagtttcc tgctcgaagg
     4741 aacgcaaaaa atcgtcggca gtcagccgct cacttacggt caatccatta ccgacccgtg
     4801 tctgggctgg gaagataccg aacgcctggt cgaaaaactc gcctctgcgg tagatacccg
     4861 cttctgaatg cgtgcccatt cctgacggaa tgggcatttc tgcgcaactt gttgtcttct
     4921 caacaaatta ctgcttgctc tggcctgcca taatattgat aataagaatc attgttatat
     4981 caattattat taatctttat gcgttatacg gatagcagaa aactcacgcc tgaaacggac
     5041 gccaatcaca aggccgcttc cccgcagccc atccggcgaa ttaccagcca gacactgtta
     5101 ggtccggatg gcaaactgat tatcgatcat gacgggcaag aatatctgct ccgtaaaact
     5161 caggctggca agctgctgtt aactaagtag cctttaactc gagcagctga cttccagccg
     5221 tttaccccag tcaggtggac gactgacata gtcatcatct ctatcgctga aaggatttcg
     5281 caacgcccca tgcaggcggt gcaattccgt catatcaccc ttttctgccg cttcaatcgc
     5341 ccgttgcgcc aaccagttgc gcaataccag agcggggtta acgctttgca tcaactgctg
     5401 acgctcacta tcgctaacct cgtcttgttg caaacgcccc cgataacggg caaaccagcc
     5461 atcaaatgcc gcacgatcaa taaactcatc acgtagcggt gacgccgtgc tgtgctgctc
     5521 ggtcagactc agcatgcgga atgtgcgggt ataatcgctg cgctctcgcg ccatcagact
     5581 gaataattca ttcagtagcg cgttatcctc tttttgctcc gtgatgaagc ccagtttctg
     5641 ccgcatccgt tgtccataat gcgtcaacaa aacctgctga tagctgtcca gggcctcatt
     5701 cagggcatct acggcaacaa atggtgacaa tgtctgcgcc agacgctgta aattccacaa
     5761 cgcgacagca ggttgattat caaaactata acgcccttga tgatccgagt gattacaaat
     5821 aaaaccgggt tcgtaatcat caagaaaacc aaacggcccg taatcaagcg tcagtcccag
     5881 cagcgacatg ttgtcggtat tcatcacccc atgtgcaaag ccgaccgtct gccattgggc
     5941 aattaacgac gcggtacgtg cgacaacatc gctaaaccag agacggtatt tgtcctcatc
     6001 atcttcaaga tgtgaccagt aatgacgaat agcaaagtca gctaactgac gaaccttttc
     6061 cgactcgcgg cggtagtaaa aatgttcgaa atgaccaaag cgcagatgac ttggtgccac
     6121 acgcatcagc atcgcgcctg gctccgccgt ttcccgatac actggcgaat cgctggtgac
     6181 gatacttaac gcgcgggtcg tcggaatgcc cagataatgc atcgcctcac tggcgagact
     6241 ttctcgtatc gtcgaacgta aaaccgcccg tccatcaccc attcgcgaat aaggcgtcag
     6301 gccagcacct ttcagatgcc agtccattgt agtgccatca gcaagcagtt gttcgccgag
     6361 taaaatgccg cgcccatcac ccagttggcc cgcccagacg ccgaactgat gaccactgta
     6421 aacctgcgcc agtggtgaca taccaggcag tagggcttcg ccgccccaga cacctgcgcc
     6481 atttttaaac agcgacgatg gaatactcag cgtgttagcc agttcggtat tatgccaaat
     6541 cagccgggca ttatttaaag gcgtagggga aagtgctgta taggtttctg gcaattcatc
     6601 gcgccagcgg gtaacaaaag acagggtcat agatcctcct gtttgatagt gtagacggtt
     6661 actctcgtct taaacaccag caaacagaag ggttatcgct gaaccaacgt cgttatctga
     6721 tttatgggaa cggcaggcca caggcatccc tggagagcat ggaaatcgaa gggggtaatc
     6781 tgtgccaata tttctgccgt atcaatgcct cccgcaatga tgcaattgca acaaggggaa
     6841 atttgcgcct ggatcgcacg aataaacggt tcgaaggagc ggtgcgtgat ttgttgctga
     6901 ataaaacttt tatccagcat gacgcgggtg aaaagaccat caaaaaccgc tttcattgtg
     6961 ctattgcctg ctcccaaatt acctaacaca aggggataca cttgcgataa ggataaaaga
     7021 ccgcgattat cttttccttc attgagatgc gggtaattct cattaatcaa cagttcaata
     7081 aacggatatt ttaatagttc gcctgcgtaa ttatccctct ctaataataa tgtagccact
     7141 tgtggcgtta aattaagcca ggcaaagagt ttatgttgaa taaaaaaatg ttggcatgat
     7201 tttagtaatt ccaactgctc tgaaaataat tgccaatgtt gctcctcggt aagttgcgct
     7261 ataacccgac tcgtcgggat acgcaccgtg ccatcttcac tggagaaatg ggtaattaac
     7321 tcaacgccga caaggacttg ctggttatct cttatcggca ggaaatagca atcagaatga
     7381 taaagattct ccaaaaaaat cttcatggta accgtccctc tcgaaggatg tttttcagta
     7441 tccggctaaa agggatgagg ccataagaca taacaattac agaaggagta actttcattt
     7501 gttccatgtt aaccactttt tcaggggtcc tttttttaga ttatcctgat tataaacgaa
     7561 taatccattt acggaatttt tgtctgcaaa atactactgt attcaggagt aaatggacgg
     7621 cgatgtatca tcgccgtcca tttttcgaca
//
LOCUS       DAJTVE010000175         7481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000175 DAJTVE010000000
VERSION     DAJTVE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OO439_003747"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OO439_003747"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6318012.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OO439_003748"
     tRNA            647..722
                     /locus_tag="OO439_003748"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO439_003749"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO439_003749"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6318013.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OO439_003750"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OO439_003750"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6318014.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO439_003751"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO439_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6318015.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OO439_003752"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OO439_003752"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6318016.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OO439_003753"
     CDS             complement(5917..6249)
                     /locus_tag="OO439_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6318017.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OO439_003754"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OO439_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6318018.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2026 c         1865 g         1767 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt t
//
LOCUS       DAJTVE010000176         7444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000176 DAJTVE010000000
VERSION     DAJTVE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO439_003755"
     CDS             <1..96
                     /locus_tag="OO439_003755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6318019.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OO439_003756"
     CDS             complement(152..487)
                     /locus_tag="OO439_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6318020.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OO439_003757"
     CDS             complement(491..835)
                     /locus_tag="OO439_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6318021.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OO439_003758"
     CDS             complement(837..1010)
                     /locus_tag="OO439_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318022.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OO439_003759"
     CDS             1111..1296
                     /locus_tag="OO439_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318023.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OO439_003760"
     CDS             1257..1376
                     /locus_tag="OO439_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6318024.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO439_003761"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO439_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6318025.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO439_003762"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO439_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6318026.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO439_003763"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO439_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6318027.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OO439_003764"
     CDS             4176..4544
                     /locus_tag="OO439_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6318028.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OO439_003765"
     CDS             complement(4652..5053)
                     /locus_tag="OO439_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6318029.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OO439_003766"
     CDS             5295..5588
                     /locus_tag="OO439_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6318030.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OO439_003767"
     CDS             5660..6319
                     /locus_tag="OO439_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6318031.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OO439_003768"
     CDS             6411..6857
                     /locus_tag="OO439_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6318032.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7444)
                     /gene="hlyE"
                     /locus_tag="OO439_003769"
     CDS             complement(7064..>7444)
                     /gene="hlyE"
                     /locus_tag="OO439_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6318033.1"
                     /translation="YAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1920 a         1671 c         1735 g         2118 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 cata
//
LOCUS       DAJTVE010000177         7394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000177 DAJTVE010000000
VERSION     DAJTVE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1520)
                     /locus_tag="OO439_003770"
     CDS             complement(<1..1520)
                     /locus_tag="OO439_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6318034.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWG"
     gene            complement(1661..3001)
                     /gene="gntU"
                     /locus_tag="OO439_003771"
     CDS             complement(1661..3001)
                     /gene="gntU"
                     /locus_tag="OO439_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6318035.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3005..3532)
                     /gene="gntK"
                     /locus_tag="OO439_003772"
     CDS             complement(3005..3532)
                     /gene="gntK"
                     /locus_tag="OO439_003772"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6318036.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3671..4666)
                     /gene="gntR"
                     /locus_tag="OO439_003773"
     CDS             complement(3671..4666)
                     /gene="gntR"
                     /locus_tag="OO439_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6318037.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(4890..5585)
                     /gene="yhhW"
                     /locus_tag="OO439_003774"
     CDS             complement(4890..5585)
                     /gene="yhhW"
                     /locus_tag="OO439_003774"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6318038.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5708..6745)
                     /locus_tag="OO439_003775"
     CDS             complement(5708..6745)
                     /locus_tag="OO439_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6318039.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7041..>7394
                     /locus_tag="OO439_003776"
     CDS             7041..>7394
                     /locus_tag="OO439_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6318040.1"
                     /translation="MLKGTFTPKRTKEERLADRPGIKQLVACIDGDVVGHLTIDVQQH
                     PRRSHVADFGICVDSRWKNRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVY
                     KKYGFEIEGTGKKYAL"
BASE COUNT         1885 a         1994 c         1779 g         1736 t
ORIGIN      
        1 tctccccaac gagaaaggac tcgccccgta gatccttgta aacccgcagg caagttccct
       61 ttaaatccgt attgcgtaaa agctccagat gctacctgtt tattgagatt taatggcagg
      121 tcagcgtgtt taataaataa atttgccgca tggagtaagc cgctatcaat atcgggcgac
      181 aacaacggat gctgaagaca actgctacat ttaccacaac gttcggcact tggctcatcg
      241 agtgcgcggc gcagaaattg cattttgcac tccttggtcg tgatatagct ttcaacatct
      301 gccagctcat tttttctctg attcatcaaa tggttaattc gttcatggtc aggagagaaa
      361 ctgacgatag ttctgcgcca taatttctcg gtatacacca caggcgatgg attttcagct
      421 accaataatt tcagtgcttt ttctatttgc ccataacgga gattcgtccg ttgttcaatg
      481 cctcgtaggg taagaccgtc attctcgcta agtacgttga gtatttcatg aatttgcgcc
      541 tccgcaggaa aggcgctttc acggaagaat ttatgaatag cgcggtcttc accaccacaa
      601 agcaatatgc caactgcgga atctatagca cgcccggcac gccctacctg ttggtagtaa
      661 ccgacaatag accccggcat ttgataatga atgacgaaac ctaaatcagg tttatcaaac
      721 cccataccta atgccgtcgt cgcgaccagc actttgattt tatttgccag cagcgcctgc
      781 tccagatatt ccctggcagt attagaatct tccgcgcctt cacaagtcac gccgctgtag
      841 taagcaaatg cactgatacc atttgtcctt aaccactcgg cgacaagttc ggcatcgcga
      901 gtagtcaggg tataaacaat tcccgatttg gaaaactgag ggataaccgt tgctaaccat
      961 gccagacggg atgactgttc acctaagact aaggcatcaa gcgccaaact ttcgcgagcc
     1021 agcgttccac gctgaatcac aatgtcaccc aattgctgac ggatatcctc aacgacacga
     1081 ttattcgctg tcgcggttgt acccagaaca ggggtattcg cagtttcaag aaaatcctga
     1141 ttcgccaacc gttcaggtga aatcagcaga caatctacac caccccgcag caacttatcg
     1201 ctaatgcgct gccattcttc cctgtttgta gaattcaacg tttcagccgt aatacctaac
     1261 cgttcagcgg cggcaacctg gttacgcatc agggcaagca aaggagagat gataattgtc
     1321 gggccagcgc cgcgatcgcg gaagatttta ctggcgatga agtacacggc acttttcccc
     1381 cagccagtgc gttggacaac cagcagtttt cgccgctgat taaccaccgc atctatagct
     1441 tcccattgcc cttccctgaa agtagcgctg gtgttactta acatccgctg taacagcaat
     1501 tcagctccgt gtttctccat catcttctcc ctcgtccgtt aacgcagtga ttagaacgga
     1561 taatgttccg gtctttttgc gagagagatc aaaaagatga agacaggaga taagagaaat
     1621 gaattactgc gagcaagctc gcagtaattt gtcgcagtaa ttaactcaac agctggaacg
     1681 caatcatccc aacaatggca ccaacagtgc cgaggatggt ttccatcatg gtccaggttt
     1741 tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa caaccagaaa ccagcgtcgt
     1801 taacgtggct gacaacaatc gaaccaccgg cgatacaaat cgacagcgcc gccatttgcg
     1861 caccggaatt gttcagttgt tcaatgaccg gcatcaccag ccccaccgcc gttaaacagg
     1921 caacggtggc agacccctga atgatgcgca ctgcagctgc cagcacgaag caggtgatag
     1981 caatcggcag gcccatgccg gttaacgctt cgcccagtgc cggacctacg ccagagtcaa
     2041 ccagcacctg tttgaacacg ccgcctgcac caatcaccag cagaatgatc cccgccggtt
     2101 gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg catgccctga cgcattgcca
     2161 ggccataaat cgctaccaga caagcaacca gaatcgcggt aaacggatga ccaataaact
     2221 cgaaccattc gtaagcggta gagccttctg gcacaaaacg cgcggcaatg gttttcagcc
     2281 ccaccagcac cagcggcaac aggatcagcg acaggctgaa tccgaaagat ggcattttac
     2341 cttcgccgag atgcggctcg ttgatgtcgt caggaatatg cagctcaacg taacggctga
     2401 tgaagttacc ccacagcggc ccggcaataa tcattcccgg aattgccgca cacaggccaa
     2461 tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc cagcagcatt ggcgctggcc
     2521 ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa taatgggatt atcagcttca
     2581 ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac gctaatcagc agaacaatcg
     2641 ccacttcaaa gaacagcggt agcgcacaga ccagccccgc aaggccgatg gcataatgcg
     2701 cgcggctgtg accgaaggat ttgagcattt tgacggcaat ctgatcgact gcgccggttt
     2761 catgtaagat cttgccaaac atagctccca gggcgacaac caccgccagg aagccgaggg
     2821 tgcctcccat ccctttttcc atcgtcgctg cgattttatc gagcggcata ccggaaaaaa
     2881 ggccagcccc catggacacc accatcaaag ccaggaaagc gtgcatacgc gccttcatga
     2941 ctaaaaacag cagcagtaaa acagacccta ctgctgttaa aacaagcgtt aatgtagtca
     3001 ctacttattt gcctttttta ataacctcaa tggtgcttgc cacaacacct tccagcggtt
     3061 gatcgatatc caccaccagt acatcggttt cgtccgcacc cggctcctgc agcgtttcaa
     3121 actgcgtcac caacatttgg gttttaaaga aatggccttt gcgcgctttc aggcggcttt
     3181 caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag attcggatta ccttcacgca
     3241 gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat cagtgacact ttattagtgc
     3301 gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca gggtttgcga tcgtcgtcat
     3361 tcagcggttc gccagacgcc attttttcga tattgcgccg tggatggagg aaatcgccat
     3421 caagaaacgc ggcatgaagt tgatgcgcca cttcactggc gaccgcagat ttgccgctgc
     3481 ccgatacgcc catcaagacg taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc
     3541 ccccacaata caagaattaa aaatgttacg ggtaactgtt atcggtaaca ttgtccagcc
     3601 ggacaatatc agaagcaata tccatccgtg ccttaagtgt ataagtgtga gctacttcaa
     3661 atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa acctaaatct aacattttcg
     3721 gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc agcgccaata ctgcccatcc
     3781 gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat cacctggcca atgtcatgac
     3841 cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa tcccagacgc tgacattcaa
     3901 acgccgcgcc gaccgccagg tcatcgttgg tacagaacac gccatccagc tgcggatatt
     3961 cccgccgcgc ctggcgaatc agttcaatac cggaagagta agaagaagat tgctcaacca
     4021 tcacgctata cggcaccaga cctgcatcca gcatcgcctg ttcgtatccc ttctgtttga
     4081 tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat gtggcaatgc ccgcgagcaa
     4141 taatggcggt ggtcatctgg cgtgccgctt caaagttatc aaaaccgacg gcgatatcga
     4201 ggcacggcga ctggctgtcc atgagttcca ccaccgggat ccccgccact tcaatcatct
     4261 ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag tccgtcgata ttccaggaga
     4321 gcatggattc gagacgttct tgctccattt ccggtttata accgtagtga gccagcatgg
     4381 tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc gcgaaaacct
     4441 ggttggtgag agaaggtaac aggacgccaa tcgcccggct ggtggcgtta gagaggatat
     4501 cgggcgcacg attgggaata tagcccagtt catcaagagc ggcggcaatc ttgccgcgta
     4561 gagcgacgga aacctgctcc gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc
     4621 ctacacggtc agccacatcc tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac
     4681 aaaagagata gattctctta gtttaacacg gacgcatcag aaccttcccg gggaaaacag
     4741 tgcttgttta caattattag atatacacta aatcaattgc atcgcattgt gctaacgacg
     4801 cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag cgagcgagga agcctgcaaa
     4861 aaggcaggct tcaaagatgc cgttgagttt taaaccggcg gcagatcgaa cagtaacact
     4921 tcgctgtcgc tatccgcatg gatggagatt gcctgctcat cccagattgc cagaccatcg
     4981 ctggtcgagg ctttcacgcc gttaatggtg acattgcctt tcaccacctg gatccagacg
     5041 cggcgttcag cggcaatctg atgcaccgac tgctcatctt tcagcaacgc ccagcggtac
     5101 agttccatat cctgatgcac tttcaatgaa ccatctcgcg catccggcga gagcaccagc
     5161 tgtttgccct gtacggcatc gaagcgacgc tgttcataac gcggcgtaat accgttttct
     5221 tcaggcatga tccagatctg atacagatgc agacgctcgg tgctgcttgg gttgtactct
     5281 gagtgacgaa tacccgtacc cgcactcata atctggaact cacccgccgg aacctgctct
     5341 ttattgccca tgctgtcctg atgctcaaca gtaccttcca gcacgtaggt caaaatttcc
     5401 atatctttat gcgggtgagt gccgaagccc tgccctgctt caatcacgtc gtcgttaatc
     5461 acgcgcagcg cggagaagcc cataaagttc ggatcgtaat agttggcaaa agagaaagta
     5521 tgccaggagt ccagccagcc atgatttgca tgaccacgtt catttgcttt gcgtaagtag
     5581 atcatttgta ttcgccccat gaatgatttc gatgggctta gtgtggaccc gatctgcctg
     5641 ggatgataga gggtgaaaat tgacccctct gttcaaaaaa attgaactat tcgaggggcc
     5701 atcaaactta cttcgcgaga gtcactgtgg agggagaggc ttgctcaaat ccgcgttcaa
     5761 ggatttccag attggtaaga acttcagatt ccttgacgta atttggcgca ccgtgggtga
     5821 tggtttgata caacgcatca taaacgcgcc cgtaatcgcc catctccggc ttcatctctt
     5881 ctctgaccgt cacgccctcg tcattgacat actccagcac accgaccgaa tcatccgctg
     5941 cgaatcccgg ttcgcccggc ataatattag ccttcaggct ggtttcctgc tggtcgatac
     6001 cgtatttaat aaacgaacct ttcttaccgt gaacgataaa tttcggataa tcgattttca
     6061 ccagatggct ggttttgacg atggctttca ggtcgccata aaacagttgc gcttcaaagg
     6121 tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc ataagcgacg tgatccgggc
     6181 gaccgaacag agaaataatc tggtccatcg tatgcacacc aaggccatag aacgcgccat
     6241 cctgcggcaa cccaggtttg gtttctgcca ccgggcggta atagtcaaaa tggctttcca
     6301 cttcaacaat ctctcccagc ttaccgcttt caatcgcttt tttcgccgtc aggaagcagg
     6361 aatcaaagcg acgattctga tatggcgtga cggtcagccc tttgcttttc gccagtgcaa
     6421 acagctcttt cgcctgcgca agtgtcggag tgaacggttt ttcgaccagc acatttttcc
     6481 cggcttccag cgcgcgcttc gcgtactcga agtggctgtc cgcatgggtg cagacaacaa
     6541 ccaacttaac atcgggatcg tttagcactt cgtcgagatc gctggtgaaa tggatatgag
     6601 agtaaatggg agcctgttct tccggcttcg catggcgacg aaaaatatgc gcgacatgcc
     6661 agctatcctt gcggttaagt atatacggca gatggtaacg ggtggtgctt ttgccgaagc
     6721 caataaaggc gcagttgatg accatgattt cgtccttttt aaggtggtta tttacacctt
     6781 agcgcaaagc ggacgtggtt cctactggag tgcgcataac gaacacaagc actcccgtgg
     6841 ataaattgag aacgaaagat caaaaaaaaa gccagcaccc ggctggctaa gtaatactgg
     6901 aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga gggtcttcct gatcgcgaga
     6961 caataataat cattctcatt cgcacttgtc caacactttt tgcaaaaaaa atgcatttga
     7021 ctcgcatttg aaagtcaatg atgttgaaag ggacatttac cccaaagagg acaaaggagg
     7081 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
     7141 tgggccatct caccattgac gtgcaacagc acccacgccg cagtcatgtt gccgattttg
     7201 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
     7261 tgattgaaat gtgcgacaac tggttgcggg tagatcgcat tgaactaacc gtgtttgtcg
     7321 ataacgcccc ggcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta
     7381 agaagtacgc attg
//
LOCUS       DAJTVE010000178         7301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000178 DAJTVE010000000
VERSION     DAJTVE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..229
                     /locus_tag="OO439_003777"
     CDS             <1..229
                     /locus_tag="OO439_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6318041.1"
                     /translation="RLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQL
                     GFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            480..2042
                     /gene="hpaB"
                     /locus_tag="OO439_003778"
     CDS             480..2042
                     /gene="hpaB"
                     /locus_tag="OO439_003778"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6318042.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2060..2572
                     /locus_tag="OO439_003779"
     CDS             2060..2572
                     /locus_tag="OO439_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6318043.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            2965..5115
                     /gene="btsT"
                     /locus_tag="OO439_003780"
     CDS             2965..5115
                     /gene="btsT"
                     /locus_tag="OO439_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6318044.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5165..5368
                     /locus_tag="OO439_003781"
     CDS             5165..5368
                     /locus_tag="OO439_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6318045.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5379..6335
                     /gene="yjiA"
                     /locus_tag="OO439_003782"
     CDS             5379..6335
                     /gene="yjiA"
                     /locus_tag="OO439_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6318046.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6413..>7301)
                     /locus_tag="OO439_003783"
     CDS             complement(6413..>7301)
                     /locus_tag="OO439_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6318047.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1682 a         1992 c         1909 g         1718 t
ORIGIN      
        1 acgcctcacg gacatctgcc gccgctttgc caaccgtccg ccaaaacggt tgatttttga
       61 caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg tgaacaatat
      121 tgcctggcaa ctcggtttta aggatccagc ttattttgcg cgctttttta atcgcttagt
      181 cggttgctcg cccagtgctt atcgtgccaa aaaagtacct gtgacgtgaa aatcccttag
      241 ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga gcaaggggat
      301 ttagtaacgt caaaaatcgc taaaagcgac cgcgatcaca aaaccgtacc cgacctgaaa
      361 agtaccagcc attgtcccaa aagtgtcttc ccgtaatcgc ccctgcaagc ttcaattgaa
      421 taacaaaaaa aacacacaaa attaacaatc gtattccgat taatactgta gaggtcgaca
      481 tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa gagtatctga
      541 aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa gacgtcacta
      601 ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac gcgctgcaca
      661 aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc ggctataccc
      721 ataaattctt ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgt gacgccatcg
      781 ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac aaagctgctt
      841 tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag aacgcccgta
      901 actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt gttaacccac
      961 cgatcgatcg tcatttgccg accgataaag tgaaagacgt ttacatcaag ctggaaaaag
     1021 agactgacgc cgggattatc gttagcggtg cgaaagtggt tgccaccaac tcggcgctga
     1081 ctcactacaa catgattggc ttcggctcgg cacaagtaat gggcgaaaac ccggacttcg
     1141 cactgatgtt cgttgcgcca atggatgcca atggcgtcaa attaatctcc cgcgcctctt
     1201 atgagatggt cgcgggtgct accggctcgc cgtacgacta cccgctctcc agccgcttcg
     1261 atgagaacga tgcgattctg gtgatggata acgtgctgat cccatgggaa aacgtactga
     1321 tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt gcccgtatgt
     1381 atccgctgca agcctgtgtg cgcctggcag tgaaactcga cttcattaca gcactgctga
     1441 aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc gatctcggtg
     1501 aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt tctgaagcga
     1561 cgccgtgggt caacggggct tatttaccgg atcatgccac actgcaaacc tatcgcgtac
     1621 tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt accagtggcc
     1681 tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg
     1741 cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca gcgcatcaag atcctcaaac
     1801 tgatgtggga cgccattggc agcgagtttg gtggtcgcca cgaactgtat gaaatcaact
     1861 actccggtag ccaggatgag attcgcctac agtgtctgcg ccaggcacaa agctccggca
     1921 atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac cagaacggct
     1981 ggactgtgcc gcacttgcac aacaacgacg atatcaacat gctggataag ctgctgaaat
     2041 aacgcagcag gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc gtgacgcgat
     2101 ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg gacaatgcgg
     2161 gattacggca acggccgtct gctcggtcac ggatacacca ccatcgctga tggtgtgcat
     2221 taacgccaac agtgcgatga acccggtttt tcagggcaac ggtaagttgt gcgtcaacgt
     2281 cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag gcatggcgat
     2341 ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc cggtgctgaa
     2401 aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg gcacacatct
     2461 ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg gacttatcta
     2521 ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa gctgcgattt aagctccagc
     2581 cccttcacgc attcgtcgaa ggggcatttt tattgtgatc tcaatcacat atccacccga
     2641 tgaataattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc ctaaaataac
     2701 cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac tgcaggatag
     2761 cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc tatgattaac
     2821 aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc cccgggagct
     2881 tacaacagta agtgacaggg gtgaacgaac gcaactgccg cacctgtaag ccaaaagacg
     2941 acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca cataccctgg
     3001 gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg tcggggggag
     3061 cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc
     3121 tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg cgcgacgcct
     3181 gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt gttgtttggt
     3241 caccacttcg ccgctatcgc cggtgctggt ccgctggtcg gtccggttct cgccgcacag
     3301 atgggccacc tgcctggcac gctgtggctg ctggcggggg tagtactggc cggtgcggtt
     3361 caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct cggtgagatg
     3421 ataaaagaag agatgggacc agtaccgggg actatcgcgc tgtttggctg tttcttaatc
     3481 atgatcatca tccttgccgt cctggcgctg attgtggtta aagccctggc cgaaagtccg
     3541 tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg tatctacatg
     3601 cgctttatcc gtccggggcg tgtgggtgaa gtctctgtca ttggtatcgt gctgctggtt
     3661 gcctctatct acttcggtgg cgtgattgct cacgatccgt actggggtcc ggcactgacc
     3721 tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc cgcattgctg
     3781 ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt
     3841 atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg
     3901 acccagtaca ttgacggtac tggcccgctg tggaaaggtg ctctgttccc gttcttgttc
     3961 attaccatcg cctgtggtgc ggtatctggc ttccacgcac tgatctcttc cggtacgacg
     4021 ccgaaactgc tggctaatga aaccgacgcg cgtttcattg gctacggcgc aatgctgatg
     4081 gagtccttcg tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc gggtctttac
     4141 ttcgcgatga acaccccgcc tgctggcctt ggcatcacca tgcctaacct gcatgaaatg
     4201 ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc acacgcggca
     4261 gcgaccgtca gctcttgggg cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa
     4321 gacattggtg agccttctgt cctgaaccgt gcaggtggtg cgccaacgct ggcggtaggt
     4381 atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg gtatcacttc
     4441 ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac ccgttctggc
     4501 cgttttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct
     4561 ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat
     4621 cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc
     4681 aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat taagatgaag
     4741 cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat ctgcaccacc
     4801 tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt cttctacatg
     4861 gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca gcagattgcc
     4921 aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat tctgttcctg
     4981 attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc
     5041 gacaaacgta ctgacaaaga aactccgtac gttccaatcc cggaaggcgg cgtgaagatc
     5101 tcttcgcacc actaaccgtg tttagccccg cttcggcggg gctttgttct tatcagagtg
     5161 aactatgttt ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt
     5221 gattggcatt ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa
     5281 gccgtacatg agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga
     5341 tggtaaaggc ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc
     5401 tactcaccgg ttttttaggc gcgggcaaaa ccaccctgct gcgccatatt cttaacgaac
     5461 aacacggcta cgagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc
     5521 aactgattgg cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt
     5581 cgcgctccaa cgagctggaa gacgcactac tcgacctgct ggataatctc gacaagggca
     5641 atattcagtt cgaccgtctg gtcattgaat gcaccggcat ggccgatccc ggcccgatta
     5701 ttcagacctt cttctcccat gagattttat gccagcgcta cctgctggac ggggtgattg
     5761 cgctggtgga tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc
     5821 aggttggcta cgccgaccgt attctgctga cgaaaaccga cgtcgcaggc gaagcagaga
     5881 aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc ggtctacacc gttacccacg
     5941 gcgatatcga cctgggcctt ctgttcaaca ccaacggttt tatgctggaa gaaaacgtcg
     6001 tcagcaccaa accgcgtttc cactttatcg cggacaaaca aaacgatatt tcgtcgattg
     6061 tggtggaact ggattacccg gtagatatca gcgaagtttc ccgggtgatg gaaaacctgc
     6121 tgctggaatc ggcggataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac
     6181 ctaaccgcct gctgttccag ggcgtccagc gcctctacag cgccgactgg gaccgcccgt
     6241 ggggcgatga aaaaccgcat agcacgatgg tgtttatcgg gattcagttg ccggaggatg
     6301 agattcgggc ggcgtttgcg ggactcaaaa agtaaccagc cctccaacat cacaatacct
     6361 gagactttat caccctcata ttcctccaga atatgagggc attcgggctt aattatctct
     6421 gaacgtgcaa cacttcctgc gactccttat gcgcctccac atcctgcttc gttaaccaga
     6481 gcgacttacg accaaaattt tcgtacattt tcagctgatc ttcatagtgc tgatcaactg
     6541 ttccatcggg agcaataaac ccactctgac cgggtgcgac cacatcccag gcaagcaccg
     6601 gacgatcgct tgtcgttggt gagaaaacaa tcatatcgtt ttctgttcca cggttttgat
     6661 actccgcctg atgacgcgtt tcttccgctg cggcctgcgg tacaccaaag aaattatttg
     6721 cacggaacgt taaggccatt gcaggtgttt tccagttact cacattattg ccatagcgtt
     6781 tggaaagagt ctcccaggta tcttccagcg cagccaacac aacctcctgc tgtggtttcc
     6841 cagcaaacag atcaaccgcc tgtgggattg gtgatttgtc tccctgcacc gcctcataca
     6901 aaatttttgc tccaacactt atattcagcg aaccagttgg gccgtcctgg gttgtttcgt
     6961 agccactggc gctgtaccac ttatcaaatg gcataggtac ggcagccact acggtacgct
     7021 tcaacatact ggtcagccaa acgttcagga tggcagagcc tggctgctgc caggttttac
     7081 catcatcatt aagcaaattg atgccatccc aacgtgttaa tgtatccacc aactgacggc
     7141 gcggatcgct ctgtgtcaaa ccagatgtcg ctgcttgcag agtaggtaaa aaaagcctca
     7201 ggttaagatc ctgacgactg gtttggcgaa taacatccca tgcctgatca gcagttaagc
     7261 gtggcttttg ctcaagcagt cggtcgatct ccgtaacgcg a
//
LOCUS       DAJTVE010000179         7002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000179 DAJTVE010000000
VERSION     DAJTVE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(115..491)
                     /gene="rnpB"
                     /locus_tag="OO439_003784"
     ncRNA           complement(115..491)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO439_003784"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(524..1669)
                     /gene="garK"
                     /locus_tag="OO439_003785"
     CDS             complement(524..1669)
                     /gene="garK"
                     /locus_tag="OO439_003785"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6318048.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1766..2656)
                     /gene="garR"
                     /locus_tag="OO439_003786"
     CDS             complement(1766..2656)
                     /gene="garR"
                     /locus_tag="OO439_003786"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6318049.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2686..3456)
                     /gene="garL"
                     /locus_tag="OO439_003787"
     CDS             complement(2686..3456)
                     /gene="garL"
                     /locus_tag="OO439_003787"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6318050.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3472..4806)
                     /gene="garP"
                     /locus_tag="OO439_003788"
     CDS             complement(3472..4806)
                     /gene="garP"
                     /locus_tag="OO439_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6318051.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5181..6752
                     /gene="garD"
                     /locus_tag="OO439_003789"
     CDS             5181..6752
                     /gene="garD"
                     /locus_tag="OO439_003789"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6318052.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6901..>7002
                     /gene="prlF"
                     /locus_tag="OO439_003790"
     CDS             6901..>7002
                     /gene="prlF"
                     /locus_tag="OO439_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCR6318053.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         1720 a         1918 c         1722 g         1642 t
ORIGIN      
        1 caggtgaaac tgaccgataa gccgctttct tttgggtata gcgtcgtgga cagtcattca
       61 tctttccgct cctccaaaag caaaaacccg ccgaagcggg tttttacgta aatcaggtga
      121 aactgaccga taagccgggt tctgtcgtgg acagtcattc atctaggcca gcaatcgctc
      181 actggctcaa gcagcctacc cgggttcagt acgggccgta ccttatgaac ccctatttgg
      241 ccttgctccg ggtggagttt accgtgccac ggactgttac cagccgcgcg gtgcgctctt
      301 accgcaccct ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg tttgctctct
      361 gttgcactgg tcgtgggttt cccccccagg cgttacctgg caccctgccc tatggagccc
      421 ggactttcct cccctccgcc cgtctccccc gaagaggacg acgacgaagc ggcgactgtc
      481 tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca
      541 ttccaatcgc cagtgtcgcg gcgatattac gtgaagcacg gcagatattg tcataagccc
      601 cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac gctgaagacc gcatcgatgc
      661 catgctgatg tacaacgcca acatcatcgg tcaggctacc cgcaatgcca atcaccggtt
      721 tatggtactt cttcgccacg tttgcaacgc caatcggtac cttgccgtga atactctggc
      781 tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc gtgaatatgt tcttccagat
      841 tcagcgccgt agtgacgatt tcaataccac ttttcagttc cgcaccaaga aacgccatca
      901 gcgccgcgcc cataccgccc gcagctcctg cgccggggac atctttcaca tcaacatgca
      961 gcgctttttt aatgacatcg gcatagtgag agaggttatt gtccagctca acaatcatcg
     1021 cttcactggc tcccttttgt gggccaaaga tgcgcgatgc gccgttatcg cccaccagcg
     1081 gattggtgac atcacaagcg acgcgaatga cgcaatcttt taagcgcgga tcgaggccgg
     1141 aaatatcaat atcattcaga gtattaagac taccgccgcc aaaaccaatt tcattgccgt
     1201 tggcgtcgca taatttcgcc cccagcgcct gtaccatgcc tgcgccgcca tcatttgtag
     1261 cgctgccgcc aatgccgata ataatgtttg ttgcactgct ctccagcgcc tgcaggatta
     1321 actcgcctgt gccgcgtgaa gtggttacga gtggatcgcg tttttccgca ggtaccagct
     1381 ccagcccact ggccgccgcc atttcaataa acgcggtttt gccatcgccg gagatccccc
     1441 aactggcatt cactttctcg cccagcggcc ctgtaaccca ggcgtgacgt tcagccccct
     1501 gggtggctgc aatcatcgct tccaccgttc cttcaccacc gtcggcaacc ggaacagaaa
     1561 catactgtgc atcaggaaaa atttcccgaa atcctttttc tatcgcctgc gcaacctcgc
     1621 tggcagataa actttcttta taagagtctg gggcgattac gattttcata cctatgcctg
     1681 ttaccacatg acgccggagg gcgtttctct tattcggcct agattccagg cccggattgc
     1741 aatacaccat ccgggcacga cgtcattaac gagtaacttc gactttcgcc agtttttcgt
     1801 agtagcacgc cagggcgcta tgatccgccg ttcctaaacc atctgcacgc agtgcctgca
     1861 tcatctccat aaccgcagct gtgagcagca gttgtgcgcc gacgccgtga gaagtatcca
     1921 gcgcattcgc cagatcctta atatgcagat caatgcggaa gcccggcttg aagttgcggt
     1981 ccatcaccat cggcgctttg gcatccagca cggtactgcc cgccagccca ccgcgaattg
     2041 cctgataaac caggtccggg ttaacgcccg ctttggttgc cagcgttaac gcttctgaca
     2101 tcgcagcaat attcagcgcc acaatgacct gatttgccag tttggtgacg ttacctgcac
     2161 cgatttcccc ggtatgcacc acggaacccg ccatcgcttt catcaaatca tagtatttgt
     2221 cgaaaatagc cttgtcgccg cccaccatca ctgacagcgt accgtcgatg gctttcggtt
     2281 caccgccgct caccggagca tccagcatat caatgccttt cgctttcagc gcttcgctga
     2341 tttcacggct tgccagcggt gcgatagaac tcatatcgat caataccgta cctggcttcg
     2401 cgccttcaat aatgccattc tcacccagcg ccacctcttt cacatgaggg gagtttggca
     2461 gcatggttat gatgacgtcg cactgttcag cgatcgcttt agccgtagac gctgtttctg
     2521 cacctgcagc aatcacgtca gcaatagctt ctgggttacg gtcagcaacc accagcgagt
     2581 aacctgcttt cagaaggttt ttactcattg gtttacccat aatccccagg ccaataaaac
     2641 caactttcat agtcatatca accatctctc ttgttgcggt ggtggttatt ttttaaaggt
     2701 atcagccagt ttctgagtgg cagagcggaa gacgccaaga tcgctgccga cagccacaaa
     2761 cgtcgcgccc cattccagat aacgacgcgc atcggcttcg accggcgcga gaataccgct
     2821 gggtttgccg tgcgcgctgg cacggttaaa aatatgctga attgcttttt gtacatccgg
     2881 atgcgaggca ttgccgagat ggcccaatgc cgcagccaga tcgctggggc cgacgaagat
     2941 accgtctaca ccttcggtag cggcaatagc atcgacgtta tctacgccct gctgactttc
     3001 tatctggacc agaatagtaa tgttcttgtt cgactgagcg aaataatccg ccacggtgcc
     3061 aaacatattg gtgcggtgag aaacggagac gccgcgaatg ccttccggcg ggtaacgagt
     3121 tgatgccacc gcctgctctg cttcctcttt tgtttctaca aaaggaatca ggaagttata
     3181 gaaaccgata tccagaagac gcttaataat taccggctcg ttggtcggca ctcgcactac
     3241 tggcgcgctg gcgctgcctt tcagggccat taactgcgga ataaacgtgg aaatatcgtt
     3301 tggcgcatgt tcgccatcca gcaccagcca gtcaaaccca gccaaaccaa gaacttcagt
     3361 gctaatcggg ttagagagtg ctgaccagca accaatttgt acctgtttcg cagccagtgc
     3421 ggctttgaat ttattcggga aaacatcgtt attcatcgct tatacctttg cttatttctg
     3481 caattccata cgtttaatgt cgccaactac gaagaggtag cagaccatcg ccatcagcgc
     3541 tgaacatccc acgaaaacca gtgctgcatt gaaggagtgc agttcactta ccaggtagcc
     3601 aatcaccagt ggagtgacaa tggaggcaac attgccaaag acgttaaaga cgccgccaca
     3661 gaggccaaca atctctttcg gcgcggtgtc agaaatcacc ggccagccca gagcaccaaa
     3721 tcctttgcca aaaaaagcca gcgccatcag catgaccacc agcgtggtgt tgttggtgta
     3781 gttacataag atgatggtgg aaaccagcaa cattcccagc acaatcggta gcttacgtgc
     3841 cagggtcagg gataaaccgc gtttgatcag ataatccgag aagacacctc ccagcacgcc
     3901 gcccgcaaaa ccacacagtg ccggaatcga ggcgaccaga cccactttca gaatcgacat
     3961 gcctttttcc tgcaccagat aaatcgggaa ccaggtgagg aagaaccagg tgatggtgtt
     4021 gataaaatat tgtccgaaaa atacgcccaa catcatgcgg ttagagagca attgcttgat
     4081 gtaatgcagt ttgggaccgc ttgctgccgc gctgcccggc tttttgtggt ccatatcgac
     4141 caccgcgcca ttttcagaga taaacttcag ctcttccgca gacatacgtg ggtgatctgt
     4201 cgggttatga atcaacttga tccacagcgc cgtcagcaca aaaccaatcc cccccataac
     4261 ggtgaagacg tgctcccagc cccaagcgaa agtcagccag ccaagcagcg gcgaaaagag
     4321 cgccagcgag aaatattgcg ccgagttaaa gatggcggag gcagtaccac gttctttcgt
     4381 cgggaaccag gcggcgacaa tacgggcgtt cgccgggaat gatggcgctt ccgagaaccc
     4441 cagcataaag cgcataaaga acatggagat ccctgcccag gccagcggga acatatcaac
     4501 aaagccttgc aggaaggtga acagcgacca gaaaaagagg ctgtaggtgt aaactttttt
     4561 cgagccaaac ttatcaagca accagccgcc ggggatttgc atcagcaagt aggcccagcc
     4621 aaaagcagag aagatgtaac ccatcgaaac cgcacttaac tgcaactctt ttgccacttc
     4681 ggtaccagca atagacagcg ttgcacgatc ggcgtagtta acggcggtaa caataaaaat
     4741 aatcagtaat attaaatagc tggtatgcac gcctttcttt tttacgtcaa cggtgtccag
     4801 aatcatttta tttacctcgg gtacttatgc tgatttttat tattatgggg aaggtgttat
     4861 ttatgagttt tatttatgcc gttacgacaa tgaactcggg aattagtata agcagcggga
     4921 gaataataat cattgtgcaa atgctaattt aatcagtact atttaaatat tattttgagc
     4981 atatgcacat aaggttgcgc gctaaagcac agatttgcgc tttaccttac cgggcggcac
     5041 tgcaatccct gaaatgattg acattgatca catttctgcg tttaaactcc tgacattctt
     5101 atttcaccca atgaagtcat ttatttttaa atgagaccag atcctcattt taataacccc
     5161 tggctggaga atattgcaca atggccaaca tcgaaatcag acaagaaacg ccaactgcgt
     5221 tttatataaa agttcacgac acagataatg tggcaattat tgttaatgat aatggcctga
     5281 aagcaggaac gcgttttccg gatgggctgg aattaattga acatactccc caggggcata
     5341 aagtcgcatt gctggacatt ccagctaatg gtgaaattat tcgttatggc gaagtgattg
     5401 gttacgccgt gcgcgcaatc ccacgcggaa gctggatcga cgaatcaatg gttgtactac
     5461 cggaagcgcc gccgttacac acgctgccac tggcaaccaa agtcccggcc cccttaccgc
     5521 cgctggaagg atacaccttt gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa
     5581 acctgctcgg catcaccacc agcgtccact gtgtggcagg cgtggtggac tatgtagtaa
     5641 aaatcattga acgcgatctg ctaccgaaat acccgaacgt cgatggcgtg gtggggctga
     5701 atcatttgta cggctgtggc gtggcgatta acgcaccggc ggcagttgtg cctattcgta
     5761 ccatccacaa tatttcgctg aaccctaact ttggcggcga agtaatggtg attggtctgg
     5821 gttgtgaaaa gttgcagcct gagcgcctgc tggtcggcac ggatgatgtg caagctattc
     5881 cagtagaaag cgccagcatt gtcagtttgc aggatgaaaa gcatgtcggc tttcagtcca
     5941 tggtcgagga tattttgcag gtcgccgaac gccatctaca aaaactgaat caacggcagc
     6001 gagaaacctg cccggcttca gaactggtcg ttggtgtgca gtgcggtggc agcgatgcgt
     6061 tttctggtgt aacggcaaac ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg
     6121 gcgcaacggt gatgttttca gaagtaacgg aagtgcgtga cgcgatccat ctgctgacac
     6181 cacgcgcagt gaacgaagag gtcggcaaac ggctgctgga ggagatggag tggtacgata
     6241 actatctcaa tatgggaaaa accgaccgca gcgccaaccc ttcgccgggc aacaagaaag
     6301 gcggtctggc aaatgtggtg gagaaggcac tcggctccat tgctaaatcg ggtaaaagtg
     6361 caattgttga agtgctgtcg cccggtcaac gcccgactaa acgcggatta atttacgccg
     6421 cgacgccagc cagcgatttt gtctgtggca cgcaacaggt ggcttcgggt atcaccgtac
     6481 aagtgtttac gaccggccgt ggtacgccgt acggcctgat ggcggtaccc gtaattaaaa
     6541 tggcaacccg caccgagctg gcgaaccgct ggtttgattt aatggatatt aacgcgggca
     6601 ctatcgctac cggcgaagag agcattgaag aggtgggctg gaagttgttc cactttattc
     6661 tcgacgtcgc cagcgggaag aagaaaacct tctcggatca atgggggctg cataaccagc
     6721 tggcggtgtt taacccggca ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca
     6781 tggccttagt gccatgtttt tattgtttaa agcccccacg tccattaata atgcatttgc
     6841 attaccttag ttcaagctta taatttgagc agaaaacagg ctgtaaaagg acaatgaatc
     6901 atgcccgcta atgctcgctc tcacgctgta ctgaccactg aatcaaaggt cacgatacgc
     6961 ggacaaacaa ctatccccgc gccagtgcgt gaggccttaa aa
//
LOCUS       DAJTVE010000180         6874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000180 DAJTVE010000000
VERSION     DAJTVE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO439_003791"
     CDS             <1..302
                     /locus_tag="OO439_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6318054.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OO439_003792"
     CDS             complement(391..903)
                     /locus_tag="OO439_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6318055.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OO439_003793"
     CDS             1098..1862
                     /locus_tag="OO439_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318056.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OO439_003794"
     CDS             1813..3216
                     /locus_tag="OO439_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6318057.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OO439_003795"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OO439_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OO439_003796"
     CDS             4573..5316
                     /locus_tag="OO439_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6318058.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OO439_003797"
     CDS             5320..6540
                     /locus_tag="OO439_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6318059.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..>6874
                     /locus_tag="OO439_003798"
     CDS             6544..>6874
                     /locus_tag="OO439_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318060.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDAS"
BASE COUNT         1819 a         1643 c         1884 g         1528 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agca
//
LOCUS       DAJTVE010000181         6854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000181 DAJTVE010000000
VERSION     DAJTVE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..289
                     /locus_tag="OO439_003799"
     CDS             <1..289
                     /locus_tag="OO439_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6318061.1"
                     /translation="VSEPKVVTVKLGDGYEQRRAAGLNNQLSTYSVKIRVRKGEHPFL
                     KAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITADFEQVVA"
     gene            299..997
                     /locus_tag="OO439_003800"
     CDS             299..997
                     /locus_tag="OO439_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6318062.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1002..1745
                     /locus_tag="OO439_003801"
     CDS             1002..1745
                     /locus_tag="OO439_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6318063.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            1682..2284
                     /locus_tag="OO439_003802"
     CDS             1682..2284
                     /locus_tag="OO439_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6318064.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2345..5824
                     /locus_tag="OO439_003803"
     CDS             2345..5824
                     /locus_tag="OO439_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6318065.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            5892..6491
                     /locus_tag="OO439_003804"
     CDS             5892..6491
                     /locus_tag="OO439_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6318066.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6555..>6854
                     /locus_tag="OO439_003805"
     CDS             6555..>6854
                     /locus_tag="OO439_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6318067.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRP"
BASE COUNT         1555 a         1648 c         2179 g         1472 t
ORIGIN      
        1 ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat ggttatgaac agcgtcgtgc
       61 ggcgggactg aataaccagt tgtcgactta cagcgtgaag atacgtgttc gtaaaggtga
      121 acacccattt ttaaaagcct ttctggaacg gcacggtggc gtccgcgcat ttcagtggac
      181 gccaccttat gactggaagc cgatcagggt ggtttgtcgt aaatggtcgg caagcgtggg
      241 ggcgttgtgg gtaaccataa cggcagattt tgaacaggtc gtggcatagg aggctctgat
      301 gcaggatatt ccacaggaaa cacatcatga gacgacacgc ctcactcagt cagcccaggt
      361 ggtgctctgg gaaatcgatc tgacagaggt cggtggtgaa cgttattttt tctgtaatga
      421 gcagaacgaa aaaggtgagc cggttacctg gcaggggcgg cagtatcagg cataccccat
      481 tcaggggacg ggatttgaac tgaacggcaa gggcagtgct gcccgtccga cactgacggt
      541 ttctaacctg cacggaatgg tcaccgggat ggcggaagac ctgcagagtc tggtcggcgg
      601 aacggtggtc aggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg
      661 aaacagcgac gccgatccgg aacaggaggt gatcagccgc tggcgcatcg agcagtgcag
      721 cgaactgagc gcggtcagtg cctcttttgt gctggccaca ccaacggaga cggatggcgc
      781 ggttttcccg gggcgtatca tgctggcgaa tacctgtatg tggacttacc gttctgatga
      841 gtgtggttac acgggcaggg cagtggctga cgagttcgac aaaccaacga cggatatccg
      901 gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg cgcaacaata ccggtaattt
      961 cggcggtttc ctttccatca ataaactttc tcagtaaatc catgacacag acagaatcag
     1021 cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgtggcttc gtggtaagaa
     1081 cgccggaggg agacaggtat cttcccagcg agaatatctc cggtgagccg gaggaacggt
     1141 tccggatggc tccggaggac tggctgcggg cacaaatgca gggtgagatt gtggcgctgg
     1201 tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg ctgcaggtgc
     1261 agagtgattt gccgtggtgg ctggtctgcc ggggggcgat tcacaagttc cgctgtgtgc
     1321 cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg
     1381 atgcttatca tctggcaggg attgagatgc cggattttca tcgcggggat gactggtggc
     1441 gtaacggtca gaatctctat cttgacaata tggaggtgac gggtttttac cgtgtcgcac
     1501 tgacagaggc acagccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc
     1561 atgccgccat ttactgcggt gacggcgggc tgctgcacca tattcctgaa caactgagca
     1621 aacgagagag gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg
     1681 catggcgcgc atctgccttt acggggattt acaacgattt ggtcgccgca tcgaccttcg
     1741 tgtgaaaacg ggggctgaag ccatccgggc actggccaca cagctcccgg cgtttcgtca
     1801 gaaactgagt gacggctggt atcaggtgcg cattgccggg cgtgatgcag gtgaaaccga
     1861 attgtctgcc cgtcttaatg agccgctggc aaatggtgca gtgatccaca tagtaccgcg
     1921 tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcgg ctgttatggc
     1981 ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt tttctctcgg
     2041 tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca gaactccccg
     2101 tacacagaca acggataacg gtaagcagaa cacgtatttc tcgtcactgg ataacatggt
     2161 tgcccagggc aatgttctgc ccgttctgta cggtgaaatg cgcgtggggt cacgtgtggt
     2221 ttctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg
     2281 ctgatgcaaa atgttttatg tgaaaccgcc tgcgggcggt tttgtcgttt atggagcgtg
     2341 aggaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa
     2401 atccacgcag ttgctgagtg tgattgatgc catcagcgaa gggccggttg acggtccggt
     2461 ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc ctggacagcg aggggaagac
     2521 aaacttttcc ggtgttacgg tggtgttccg tgcaggtgag caggagcaga caccgccgga
     2581 gggttttgaa tcctccggct ccgagacggt gctgggtacg gaagtgaaat atgacacgcc
     2641 gatcacccgg accatcacgt cggcaaacat cgaccgtctg cgcctgacct tcggtgtgca
     2701 ggcactggtg gaaaccacct caaagggtga ccggaatccg tcggaagtcc gcctgctggt
     2761 tcagatacag cgtaacggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac
     2821 cacttcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc cgtttaatat
     2881 ccggatgcgc aggatgacac cggacagcac cacagaccag ctgcagaaca aaacgctctg
     2941 gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgctggtcgg
     3001 cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg agtcgcaatt atcatcttcg
     3061 cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat
     3121 ctgggacgga acgcttaagc cagcatacag caacaacatg gcctggtgtc tgtgggacat
     3181 gctcactcat ccgcgctacg gcatggggaa acgtcttggt gcggcagatg tggacaaatg
     3241 ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg acggacggat ttggcggcac
     3301 ggagccgcgc atcacctgta actcttacct gaccacacag cgtaaggcgt gggatgttct
     3361 cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacggacaga tgctgacgtt
     3421 cgtgcaggac cgaccgtcgg ataaggtgtg gacctataac cgcagtaatg tggtgatgcc
     3481 ggacgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc ataatgccgt
     3541 tgaggtgaac tggattgacc cagataacgg ctgggagacg gcgacagagc ttgtggagga
     3601 tacgcaggcc attgcccgtt acggtcgtaa tgtcacgaag atggatgcct ttggctgtac
     3661 cagccggggg caggcacacc gagccgggct gtggctgatt aaaacggaac tgctggaaac
     3721 gcagaccgtg gacttcagcg tgggcgcaga agggcttcgc catgtaccgg gtgatgtcat
     3781 tgaaatctgc gatgatgatt atgccggtat cagcaccggt ggtcgcgtac tggcggtgaa
     3841 cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccgtcct ccggtaccac
     3901 gctgataagc ctggttgacg gaagtggcaa tccggtcagc gtggaggtcc agtccgtcac
     3961 cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc gttgcagaat acagcgtgtg
     4021 ggggctgaag ctgccgacgc tgcgccagcg tctgttccgc tgcgtgagta tccgtgagaa
     4081 cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag aggccatcgt
     4141 ggataacggg gcacactttg acggtgaccg gcgcggcacg gtgaatggtg tcacgccgcc
     4201 agcggtgcag catctgaccg ccgaagtcac cgcagacagc ggggaatatc aggtgctggc
     4261 gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc tgaccgtggc
     4321 agcggacgac ggcagtgagc ggctggtcag cacagccagg acgacggaaa ccacataccg
     4381 cttcaggcag ctggcgctgg ggaattacag tctgacagtc cgggcggtaa atgcccgggg
     4441 gcagcagggc gatccggcgt cggtatcgtt ccggattgcg gcaccggcag cgcctgtcac
     4501 tattgaactg ataccggggt attttcagat aacggcggtc ccgaaactgg ctgtatatga
     4561 cccgacggtg cagtttgagt tctggttttc ggaaaagcgg attgcggata tcaggcaggt
     4621 tgaaaccagc gcccgctatc ttggcacggc gctgtactgg atagccgcca gtatcaatat
     4681 caggccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg gcaaatcggc
     4741 atttgtggag gccatcggtc gggcgagcga tgatgcagaa ggttatctga atttttataa
     4801 agggttgatc aataaaacgc atctcggcaa ggagttgtgg acgcagattg ataacggtca
     4861 gcttgcgccg gacctgactg aaatcaggac gtccattacg aatgtcagca atgaaatcac
     4921 gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca atccagcaga tacagaaagt
     4981 tcaggttgat acaaataata acctgaacag catgtgggct gtgaagctgc aacagatgaa
     5041 ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaatacgc cagcaggaat
     5101 gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg cgaatggcaa
     5161 cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg tgttcctgaa
     5221 atgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt ccctgacacc
     5281 tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg cgaactccgg
     5341 gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat tgtccgcgaa
     5401 ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg actcccgggc
     5461 accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc agccgtttga
     5521 tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc ggaagaataa
     5581 caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa tttataaccg
     5641 aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg ccggtcacgg
     5701 tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt atcccacagc
     5761 aagtatcagc gatttgctgg ttgtggtgat gaagaaagcc actgcaggca tcacgattag
     5821 ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt gcagaaaagc
     5881 gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc cgcagccatc tgtctggccg
     5941 tatccggtgc gcctgcatgg gcgtctgaac atcagtccac gctgagcgcc gggtatcttc
     6001 agacccatac tgatatgcca ggcagcgatg acctgaaggg cattaacgtg aaataccgtt
     6061 atgagtttac ggacacgctg gggctgatta cgtcattcag ttatgccaac gctgaagatg
     6121 agcaaaaaac gcattacagc gatacccgct ggcatgagga ttccgtgcgt aaccgctggt
     6181 tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg
     6241 cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc gtaactgaca
     6301 acaaggggaa aacgcacgat gtgctgaccg gaagtgatgg cggtcgccac agcaacacgt
     6361 ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc attgaccttg
     6421 cttatgaagg ttccggcagt ggtgactggc gcactgacgg tttcatcgtg ggtgtcggtt
     6481 ataagttctg attagccagg taacacagtg ttatgactgc ccgctggttc aggcgggctt
     6541 tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg gcgggtaaac
     6601 ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc gttgtggcac
     6661 acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag gctgagccgg
     6721 ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc ggggatattg
     6781 atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca ccaaaggatg
     6841 gtgatttacg tccg
//
LOCUS       DAJTVE010000182         6762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000182 DAJTVE010000000
VERSION     DAJTVE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..204)
                     /locus_tag="OO439_003806"
     CDS             complement(61..204)
                     /locus_tag="OO439_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCR6318068.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(302..553)
                     /locus_tag="OO439_003807"
     CDS             complement(302..553)
                     /locus_tag="OO439_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCR6318069.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(621..2291)
                     /locus_tag="OO439_003808"
     CDS             complement(621..2291)
                     /locus_tag="OO439_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCR6318070.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPDLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2524..4743)
                     /locus_tag="OO439_003809"
     CDS             complement(2524..4743)
                     /locus_tag="OO439_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6318071.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4922..5188
                     /locus_tag="OO439_003810"
     CDS             4922..5188
                     /locus_tag="OO439_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6318072.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARIRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5402..6328)
                     /locus_tag="OO439_003811"
     CDS             complement(5402..6328)
                     /locus_tag="OO439_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6318073.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1597 a         1889 c         1547 g         1729 t
ORIGIN      
        1 aaaaaaaagc gcgtcagaat cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg
       61 ttactgcgtt gctaatccgg tcagttcagc tgaggatgac ggggatacag tcccgccctg
      121 aacatcgcgg atatagtttg cctgacatgc agcaagaata atggctgcaa gaagcaataa
      181 aataatccct gttatttttt tcatactgtt ccttgtatat atggactcag gcattcatcc
      241 ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa cataaaattc tataacacta
      301 tttacatctt taacgtgata atgaaagcag tgtatccatt ctcaatatca tctttaatat
      361 tttcaatagc caatctatta ttttcaggga tttttgattt gtattcctcc ggcatatcat
      421 atgtccatga tgctgaacta tctatcctga atccaggatt cagccaatgc ccaccatcaa
      481 tgcccaaaat ccataagcct tttttcgctg cttcattaca aacttctatt gcagcttttg
      541 gtgataactt catagaagca tttcctttta attcaaaaaa ggcatcacca ctatcatata
      601 acaccgtatg ttcaaataat ttattggtca tttttatttc cccattgaat tctcgaatcg
      661 tccacccaac ccggatctgt cttatcacta atctgagtaa cctcaccagt acgatcattg
      721 acaacaacat atttaccagg agaaccatac actgttgctg gatcatttcg acctaaatag
      781 tctggaggtg ttttcttcgg actacgctta tcaaaagatg taccagttgc accgttagat
      841 acggtatttt tgatatcatc ctctgtccat cctctttctg gcatttgtcc cctaattttc
      901 tgatctattt ttagatctgg tatctttcct ttattttttg ctaactcatc atttagtttg
      961 ccttcagcat ctcttttatt atcctctttc ttcttcctgc tttccatcgc agtacttaat
     1021 gcagcatcag catctgcctt ctcttttgca gcagcatcaa atgcagcctg cttattattt
     1081 acatccgtct gcgcccgctg ggctttaagc ccggccattt gccacattct gtgaccgcca
     1141 gccattgggt catgtgcaaa tcgttcaaat tgttttattt cagctattgc atcagcaaga
     1201 gttttattcg ctgcatcaag ttcgctttta cgcgaattat aaacctgaac agctttagcc
     1261 tgtcgctcct gatttctggc aacatcttca tttgcctgat tcagctctgc acgcgcgcgt
     1321 tcataatttc gctcagccgc ttcaaccgga tgcgtagcat cccattcctg ctgacggcga
     1381 ttttcttcat cctgacgttg ttttacctgg tcagggctaa gaacatcact cactgaaaca
     1441 tatacggcat tatgaccgct gtccttcggg aatcgaataa ctgcatccct ggtattaccc
     1501 ccctgagtaa atcctgccgg gcgaacatcc ttatcagtat tatttgtaac acctgggctt
     1561 aatgtctgta ctgctggcgt actgttatta actgaaatat tcagaacagg tgcacctgga
     1621 attgatgccg taaaaacacc tggacgttcg gtaggttttg catcaaccac cggaacactc
     1681 atcggaacac ctgaaacaac cgaaatattc tgtcgttcgt cttttacatc atcaacaaca
     1741 cgaacattta cgtttactgt tgccttatcg agaggtaatg aactgacagg tgattcagta
     1801 atatcatctg cgggtaatga cgtcacaatc tttgacatca tattggggtc atctttcgct
     1861 atttgtgatg gcaatacacc atataaagcc accccccaaa gaccaaattt aaacggtcct
     1921 ttcagggcag ccataatatc agcaatagct gccgataatg ctcccgctga aatactgacc
     1981 gccagaccgc cagcacctgg agtggaaagt gccggaaaac caaatgccac tggcgcagct
     2041 actgctgaca gattaccgcc tgttcccgag ccaccaccgg aattaccatt ccccccgcca
     2101 ttaccatgac cggaaccacc tccccagtga atgccgctac cggaaccacc accccacggg
     2161 ttattttccg aactccaccc ggaaccatca gaagcaccac cacctacacc aagcccggtc
     2221 gggccaccat taatgttacc acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg
     2281 ccaccgctca taaaaattcc ctcttttaag cgttaaaaca attaattaca cgattaatac
     2341 tgtacataaa accactggtt ttatgtacag atcaaaaagt acccgtcatc gttttttctg
     2401 tcaagctcag gtggaaaatt ttttagaaat caggagggat aacacttaga aaacgcagag
     2461 cgggccacaa aaaattttgt ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg
     2521 acatcacata ctcatgcccc ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc
     2581 gtcccgcttc cactcatgcc aggtctgatt aaaaccgagt tgacggaaga gataatccgg
     2641 catctcttcc ggtttgtgga cagcagccaa aagcctgtca tgatggcctt tgagccggaa
     2701 ctgcatcagg tgcggttccg gtgggtacgg agtatcctca tcatggttta tagcttctcc
     2761 gtaacctgcc aggcataccc cgttctccgt gatggtgctg actatcagat gactgtaatc
     2821 cggcgacgga agcacaagaa cggggcaaaa ccctccttcc cggcgaacaa acccgacaag
     2881 catttcctcc ggccctttgc ataacaacag attcagcgtc ctgctgtttc cctctctcga
     2941 aaatacaaca ggcatgagtt gttccggtac agcatcagcg gtgatatcca cccggtgcgc
     3001 tgacggcagc ggagaggcta cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca
     3061 gtgatgcggc agcgccggaa aaccgtaaga gggccacccc tggcgcatct gttccagtac
     3121 atgccggacc gtcgcaccgg aatacattct cacacgctca ggccgcgcca tcgtctcatg
     3181 agattctggc attccctcac gtatatccga gaccatcaca gccccgcctg gcgacaggta
     3241 aacattcacc gggtcatgaa cactctgggg gtaatgcgcc agctcatcac cccataccgt
     3301 gaattctccg gctctgttac tcagcatcat gtagcgggtt atcctgcccg ttcccggctc
     3361 tctggcacgc cccagctccg taataacccc gactaatgcc gtttcacggc tcagacggtg
     3421 gttatcatga cgtttcagcc gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc
     3481 ggcttcctcc ctgcgccgct ccgcttcccg tgctgacagt ttttctttca gctctgccat
     3541 tcccggcgca atccacgtat tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc
     3601 ccgttctttc acggccctgt aggtgcggcg aagagattca atctgtttcc cgagtttcca
     3661 gaaacggagt tgtcttttgc ggtattccgg ttcagcccat gtcatcaggg ggatctctga
     3721 cagaagggaa gcgacagggc gtgctttttt gagtccggtc atctcctttt cgagaatggc
     3781 ctgctctttc agcagcgcat tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc
     3841 caggaactcc ggggtcatct cccacgcgtt gccgcattcc tgcggcaacg tggcaatgaa
     3901 ttcccgcagg gtatccgctg aggcattcgc agcgggaatg gtcaggttaa gggcttccgc
     3961 tgcacgacgt gcggcatcgc tgagttcccg tttccgcacg ttctcctgta cctggatgac
     4021 tgccagcctt gtgcgggaaa cctgtatttc caggttaagg ctgttaatca gacggttctg
     4081 ctcaccgcgt tctgtttcca ttccccgttt ttccatcgca caggcagcct ttccgaggtg
     4141 aaggccgggc gctttctcca gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg
     4201 gtagccggcg ttcgcaagcg ctctgttcgc atggtcagcc catgatgcgc gccacgtctc
     4261 ggcgagtgcc cggtcgttcc agtccctgac ctttcctgca aatcccgttt ccccgacagc
     4321 tctcgtggtc agcatgatgt gcgcatgggg attggtccgg tccatgtgat ggaacgccac
     4381 atcggcaatc atgccccgac tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg
     4441 tgcggcctcg cgggaaatct cacggggaat ggcgagttca atctcccttg ccagctggga
     4501 gttccggcgc tgttcggctt tctcgaccgc attccagaga acggaacggt caccacacca
     4561 ggacggtgca ttcgcagggg tgagaatgac cgcatcatca acgccgcgtt tacgggtgta
     4621 atcgtgggtt aatccggtac ggtcatcggg tatgacagag ccggaacggt aggcagcaga
     4681 agcaacggca ctgtagccgt tttttcgcga aatgattttc atgctgagat gataaatcgc
     4741 catttctgcc gtaatgcctt tttgccgttt tcttttagcg tcgcagaccg cacacaatcg
     4801 aaacgaagtg gagattgtat aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc
     4861 agttttgcaa ctaccagtga cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa
     4921 aatgagcaga acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa
     4981 ggcgaagagc cggagcctgg acacggcgca aaaagtcatt gtgggggctg ccatgctggc
     5041 gaggatacgg aggccggaag aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa
     5101 ggaagtcaca agacaggccg atgtgaaccg gatacaaccg ctgattaacg agctggagaa
     5161 gctacccaaa ccacctgcga aaccgtagaa cggagaaacc ccggcgcgta agtgccacat
     5221 gcaccaccga cacctttgcg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     5281 gcggcgagcg ctacaacctc aatcatgcgg aaagccgcac cattgcaggg aggaaacgaa
     5341 aaagccacca aacgccaaca gctcaaaaaa gcgctgttat ctgatatagg ttttatttgg
     5401 tctactctac aagacctcgt tttttcagtt ggtaatacca ggcgcggcta attccaagag
     5461 tctcccacgg tttcaacgtt cgcgcagatg tggcaacagc accacgctta gacttagcac
     5521 ctccaatttt cccgcccttg cgaccacgtg cagcctgaat ttctggcgtg tgcgtatcag
     5581 ccacatactg tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatgctct
     5641 tgccaatagc ccgacattcc gccggagaaa gcggaacggg aagcgatacg ttgtacattt
     5701 cgacacgctg gatcacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
     5761 ccctgtaagc ccatttgcgc gtcatttcga acaggtggca gtttcgcccc atcccgtaat
     5821 gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga
     5881 gggtataggc ttcctcgcgc cactccatca ccagccattc caggtggaac ggatttttgc
     5941 aaatcagtcc gctgtagttc acatctgcac caagtttctc acacagcgca cgctctatcg
     6001 cggcagcgta cttcagtgcc ttaaccgacg catcaggcgc ggttctcacg gcgatgttga
     6061 gtgcatagag caggtgagca tgaccgttca cgggattttt tacggtgatg ttcggcgcgg
     6121 gggcgttccg gtcgctccag tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc
     6181 agaactgcgc atgaggctgg ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc
     6241 cggagatgcg aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt
     6301 cttcgaagta ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac
     6361 ctcgcccctg tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt
     6421 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
     6481 tccatttacc ataactcccg ttgtacgtac cccttaaaac gccctcaatc gctttctgac
     6541 gcaaaaaacg caaaaagaca aggaaacaca gggtaaacat aaaaaaatgc gtcagaatcg
     6601 cttttagcgc gttgcataac tatgcacgaa agcgatgcgc gatcggtttc gtgcgttctg
     6661 aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt ctgcacgagg
     6721 attttagatc ccatctccca catggaattt ttgtgacact tc
//
LOCUS       DAJTVE010000183         6669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000183 DAJTVE010000000
VERSION     DAJTVE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            228..853
                     /gene="gstB"
                     /locus_tag="OO439_003812"
                     /pseudo
     CDS             228..853
                     /gene="gstB"
                     /locus_tag="OO439_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(850..1965)
                     /gene="yliI"
                     /locus_tag="OO439_003813"
     CDS             complement(850..1965)
                     /gene="yliI"
                     /locus_tag="OO439_003813"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6318074.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2076..2459)
                     /gene="bssR"
                     /locus_tag="OO439_003814"
     CDS             complement(2076..2459)
                     /gene="bssR"
                     /locus_tag="OO439_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6318075.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2672..3997
                     /gene="rimO"
                     /locus_tag="OO439_003815"
     CDS             2672..3997
                     /gene="rimO"
                     /locus_tag="OO439_003815"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6318076.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4044..5365)
                     /locus_tag="OO439_003816"
                     /pseudo
     CDS             complement(4044..5365)
                     /locus_tag="OO439_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5373..>6669)
                     /locus_tag="OO439_003817"
     CDS             complement(5373..>6669)
                     /locus_tag="OO439_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6318077.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1768 a         1664 c         1516 g         1721 t
ORIGIN      
        1 attcaaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata acgcacacac
       61 aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt
      121 cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt tacttcacca
      181 tcggagtcct gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg attacgctgt
      241 ggggtcggaa taattcaact aacgtaaaaa agtattgctg acgctcgaag aactggaact
      301 accttatgag caaattctcg cgggccgtga gtttgggata aatcacgatg ctgattttct
      361 ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg atctcattct
      421 ttgggaatca aacgccattg tccgctatct ggcggcacag tacgggcaaa aacgcctgtg
      481 gatcgactca ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg caaaccagac
      541 gctcagtaat gctcatcgcg ggatcctgat gggattagtc agaacaccac cggaagagcg
      601 cgatcaggcc gccattgatg ccagttgcaa agagtgcgac gccctgtttg ccctgctcga
      661 tgcggaactg gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg gcgatatcgc
      721 tatcgcaccg ttcatctaca atttgtttaa cgttggcctg acctggacac cgcgtccaaa
      781 tctgcaacgc tggtatcagc aactcactga acgccccgca gtgcgcaaag tggtgatgat
      841 tcccgttagc taattgcgtg ggctaacttt aagtaattcc ccactggact cgtcggtgag
      901 aacgtataaa taaccgtcgg gtccagtgcg aacatcacga attcgctgcc ctctgtccgt
      961 taaaatacgg ccatcttctg tcactttgtc gccgttgacg ctcatcacaa tgacatcttt
     1021 atctttcagc gcgccaataa ataatttttg ctgccactgg gggaatttat cgctgttata
     1081 gaaggccatg ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc
     1141 ggcgacgatc tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt
     1201 tgccagcggc cagccgtagt gtttgccttt ttgcgggata ttaatttcat caccaccgcg
     1261 cgggccatgt tcattcagcc acagtgcatt actccacgga ttcatcgcca ttccttgcgg
     1321 attacgaatg ccataagacc agatctcggc gcgcgcaccg gattccttta taaaaggatt
     1381 atcatccggg atttcgccct ggtcggtcag acgcaccagt ttgccctgta atttatccag
     1441 atcctgcgcc gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc
     1501 gtcgaatacc agccgcccgc caaaatggtt gccggtagac agttttggca tctggcggaa
     1561 gacggtgcgg aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc
     1621 agttccggct ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg
     1681 agcaaaatca ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc
     1741 cggaactccg gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc
     1801 gccgcgcaga gtgattaaca taccgtgatt atcgggtaaa aaggccagtg cccagggatg
     1861 gtcgagtttg tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga
     1921 agaaagacaa ataaggggca caaggaaaaa ggattgtcga tgcataacgc ctccggattg
     1981 actctggctt aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa
     2041 acatgaaggg ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc
     2101 ttctgcaggc tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg
     2161 ttgacggtaa taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc
     2221 gctaatgctt cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc
     2281 aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga
     2341 ttgctttcgc tgacggacat gtatcctttt gccttcctca gttgcacgta tgcagcgagg
     2401 tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata
     2461 cttcgatcct cctctttcca gccttgttgc gattaaatag tatggctatt ttttgagcaa
     2521 aatgctgtcc aggatcaatt cttcacgcca aattaacaaa atttacattt ccttcgcctc
     2581 cgccagcgaa tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg
     2641 acctttcctg taactgaata ttttttaagc tatgagcaaa gtaactcccc agccgaaaat
     2701 cggctttgtt tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga
     2761 actacgcact gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt
     2821 caacacctgc ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc
     2881 gttgaatgaa aacggcaagg taattgtgac cggttgtctg ggggcaaaag aagatcagat
     2941 ccgcgaagtc cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct
     3001 ggagcacgtt catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc
     3061 agaacaaggt gtgaaactga caccgcgtca ttatgcctat ctgaaaattt cggaaggctg
     3121 taatcaccgc tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc
     3181 gattggcgaa gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct
     3241 ggtgatctcg caggatactt ccgcctatgg cgttgatgtt aaacatcgta ctggcttcca
     3301 caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg
     3361 gatctggaca cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact
     3421 gatggcagaa ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg
     3481 cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca
     3541 gtggcgcgaa atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctag
     3601 cgagacggaa gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg
     3661 cgttggctgc tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca
     3721 ggttccggaa gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat
     3781 ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt
     3841 ggacgaagaa ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt
     3901 ttatctcaat ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca
     3961 cgccgatgag tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca
     4021 tttgtccgcc ctgcgaataa tgattactgc cagctaatct gagccctgcc gtttttctta
     4081 ctcttataca gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt
     4141 gaatgcgcta caccgatact ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt
     4201 tcttttagaa tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg
     4261 tcaccgccga agcggtagat tttaccttta tctaccggga tctgtgattt aaggattttc
     4321 accactttca tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg
     4381 aatttatcga cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct
     4441 tcgtcaaagg attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac
     4501 atactgtaga gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt
     4561 tcaaaatggt tattaatgat ggcttctttc tccacaatac cacgattaat cactctgcgt
     4621 accagataga ggcaactggt acccgatatc actgccacca atgcggcaaa gaaaataatc
     4681 aatgtacaga cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc
     4741 aaataggcga agtgctccat cgcttccgaa acggtgaaga tggcgttttt agcattatat
     4801 tcatcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg tccatcgcca
     4861 gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt
     4921 gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat
     4981 ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact
     5041 cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag
     5101 agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt tcctccgtgg
     5161 gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca
     5221 ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt
     5281 gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa
     5341 gtacgaactt attgattctg gacatacgta aactactctt ttactaattt tccactttta
     5401 tcccaagcgg agaatggcat gggtttctgg tacagatacc cctgagcctg aacacatcct
     5461 aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc
     5521 cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata
     5581 tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc
     5641 aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt
     5701 tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca
     5761 acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga
     5821 aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc
     5881 agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa
     5941 cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg
     6001 atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg
     6061 ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga
     6121 atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc atcacagaaa
     6181 ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg
     6241 taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga
     6301 ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc
     6361 tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag
     6421 cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt attgcgttca
     6481 tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc
     6541 agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc gctttcctga
     6601 tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc
     6661 gcgatcgaa
//
LOCUS       DAJTVE010000184         6638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000184 DAJTVE010000000
VERSION     DAJTVE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..727)
                     /locus_tag="OO439_003818"
     CDS             complement(<1..727)
                     /locus_tag="OO439_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6318078.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQ"
     gene            complement(868..2901)
                     /gene="metG"
                     /locus_tag="OO439_003819"
     CDS             complement(868..2901)
                     /gene="metG"
                     /locus_tag="OO439_003819"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6318079.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3033..4142
                     /gene="apbC"
                     /locus_tag="OO439_003820"
     CDS             3033..4142
                     /gene="apbC"
                     /locus_tag="OO439_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6318080.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4387..4668
                     /locus_tag="OO439_003821"
     CDS             4387..4668
                     /locus_tag="OO439_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6318081.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4911..5453
                     /locus_tag="OO439_003822"
     CDS             4911..5453
                     /locus_tag="OO439_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318082.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5533..6207
                     /locus_tag="OO439_003823"
     CDS             5533..6207
                     /locus_tag="OO439_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6318083.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6223..>6638
                     /locus_tag="OO439_003824"
     CDS             6223..>6638
                     /locus_tag="OO439_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6318084.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1752 a         1505 c         1594 g         1787 t
ORIGIN      
        1 gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg acgatctgat
       61 ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc gtgagtacag
      121 ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg atcgcctggg
      181 cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct gttttattat
      241 cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct gcttgctgaa
      301 caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg ggcggaaaaa
      361 cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg tcaggcgcac
      421 gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc gctaccttga
      481 gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc tccacatacc
      541 ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc gcagcatctg
      601 aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg atatgcaact
      661 cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga tagataaagt
      721 gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc agtatttatg
      781 cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa agaaaaggcg
      841 ctgtcgatgc agcgccttga agagggatta tttcacctga tggcctggtt tagcaccagc
      901 atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta ccatgccttc
      961 ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg tgtgacgccc
     1021 gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat tgcgtttttc
     1081 accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa actctgcgtt
     1141 ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga tggtttcctg
     1201 aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca cttcttcttt
     1261 agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca gcgctttgaa
     1321 cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca gttctgtatt
     1381 gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt aagtcatcag
     1441 cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat cgcggccttc
     1501 ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca gatcagccag
     1561 cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt caccaatcac
     1621 ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc ttgccagcac
     1681 gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa ccactttgtt
     1741 cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga tatcatcaat
     1801 gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa aatgattcag
     1861 ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc cgttcaccgt
     1921 cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt ccagcatggc
     1981 aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt acagctcggc
     2041 ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct tgtcgcacag
     2101 attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga aatatttgcc
     2161 cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat cccactgttg
     2221 caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca acgcaccgct
     2281 gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga agtgttcaga
     2341 atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca attcagtcgg
     2401 gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg atttacattt
     2461 cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg gatcgtacag
     2521 ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt agataagttc
     2581 tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc tgatgttaaa
     2641 gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct ccggggtgat
     2701 accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg cgcagatgaa
     2761 gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct ggatgtgctc
     2821 cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg tcaccagaat
     2881 tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag gggttagata
     2941 gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta aactatcagc
     3001 tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag gccaaatcac
     3061 cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac ccaaccctga
     3121 agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac gacacgttgc
     3181 atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta aaagagcaat
     3241 gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa ctgtcgcaca
     3301 atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg aagaacatca
     3361 ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta aacctggcgc
     3421 tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc tacggcccat
     3481 caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac ggtacccata
     3541 tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg gtcactgacg
     3601 acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag atgttgcagg
     3661 aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc accggtgaca
     3721 ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc accaccccgc
     3781 aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa gtcgaggttc
     3841 cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt ggtcaccatg
     3901 aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac acacagctgc
     3961 tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc acgccaacgg
     4021 ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct gaccgcgttg
     4081 cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc cgcgcggtct
     4141 aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac attgatgcgg
     4201 tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat tctgcggacc
     4261 ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt atcatccttc
     4321 gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     4381 ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg attggcttct
     4441 cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag tcccccaaca
     4501 tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt aacgtataat
     4561 gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga agtggttgtt
     4621 gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt tggcaacctg
     4681 atttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4741 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgtttcaaa
     4801 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4861 gagatacaga ctcttaacaa ccaaaaaaaa attttattaa ggaacatatt atgaaacgtt
     4921 caattattgc tgccgctgtc ttttcttctt tttttatgag cgctggagta tttgctgcag
     4981 acgttgatac cggaacatta actattaagg ggaatattgc agaatctccg tgtaaattcg
     5041 aagcgggtgg tgattcagta agtattaata tgccgactgt accaaccact gtctttgaag
     5101 gtaaagctaa atattctacc tatgatgatg cagtcggtgt aaccagcagc atgttaaaaa
     5161 ttagctgccc gaaagaagtt gctggtgtaa aactctcgtt gattaccaac gataaaataa
     5221 ccggtaacga taaggcgata gccagtagca acgataccgt gggttactat ctctatttag
     5281 gtgataacag cgatgtcctg gatgtttctg caccttttaa cattgagagt tataaaacag
     5341 cggaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag
     5401 tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa
     5461 aattgtaagt ttctccaggt aataaacatg gccgctatcc catggcggcc ttttaattta
     5521 agaggcatta aaatgaaagg attattatct ttactcattt tttctatggt ccttcctgca
     5581 catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa taaagaagtg
     5641 atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg gattgatgat
     5701 ggcgatacgt cattaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg
     5761 gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg
     5821 cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc aaatagtcca
     5881 gagcaagaag gtaagaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac
     5941 cgaccagcgg gtattgctcc ggtaaataaa gcgacattta aaaaattgct ggtaaatcgc
     6001 agtggcaatg gtttggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc
     6061 aaagctaata atgtcaaagt caattatgaa actattatga ttgccccctt agaaagtcag
     6121 agtgttaatg tcaaaagtaa taatgcaaat aactggcatc tgaccattat cgatgaccat
     6181 ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca
     6241 cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc
     6301 tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt
     6361 gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa taagcaatgg
     6421 cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt atcaagagaa
     6481 gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca
     6541 tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt tcgtctcgat
     6601 ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaag
//
LOCUS       DAJTVE010000185         6622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000185 DAJTVE010000000
VERSION     DAJTVE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..605)
                     /locus_tag="OO439_003825"
     CDS             complement(24..605)
                     /locus_tag="OO439_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318085.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO439_003826"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO439_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6318086.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5874)
                     /locus_tag="OO439_003827"
                     /pseudo
     CDS             complement(4870..5874)
                     /locus_tag="OO439_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5859..6236
                     /locus_tag="OO439_003828"
                     /pseudo
     CDS             5859..6236
                     /locus_tag="OO439_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6275..>6622)
                     /locus_tag="OO439_003829"
     CDS             complement(6275..>6622)
                     /locus_tag="OO439_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6318087.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1644 a         1471 c         1494 g         2013 t
ORIGIN      
        1 acctcaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcactc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctacggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccactagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgag
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacttgg tgtttgtatt ttgaactttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaact cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     6121 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     6181 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     6241 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     6301 atgattgggc agcaagtgca atatcccaca taaatccagt taactcacga gccaccgccg
     6361 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     6421 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     6481 atataagcca tgctccaact tttgcaggtg tacgataaga ccaagcggct tcgtaaagca
     6541 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     6601 tggaatattc acctggcacc ag
//
LOCUS       DAJTVE010000186         6533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000186 DAJTVE010000000
VERSION     DAJTVE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..905)
                     /locus_tag="OO439_003830"
     CDS             complement(<1..905)
                     /locus_tag="OO439_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6318088.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFD"
     gene            1601..3886
                     /gene="nrdA"
                     /locus_tag="OO439_003831"
     CDS             1601..3886
                     /gene="nrdA"
                     /locus_tag="OO439_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6318089.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4120..5250
                     /gene="nrdB"
                     /locus_tag="OO439_003832"
     CDS             4120..5250
                     /gene="nrdB"
                     /locus_tag="OO439_003832"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6318090.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5250..5504
                     /gene="yfaE"
                     /locus_tag="OO439_003833"
     CDS             5250..5504
                     /gene="yfaE"
                     /locus_tag="OO439_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6318091.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5581..6207)
                     /gene="inaA"
                     /locus_tag="OO439_003834"
     CDS             complement(5581..6207)
                     /gene="inaA"
                     /locus_tag="OO439_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6318092.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6386..6533
                     /locus_tag="OO439_003835"
                     /pseudo
     CDS             6386..6533
                     /locus_tag="OO439_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1527 g         1634 t
ORIGIN      
        1 atatcaaagg taacttcact taagccgaga tacataaagc cacccgccgc agtggaagga
       61 ccatctccat agcctgctgc gctgttgttc tcatccacta acacgccgcc gttctggctg
      121 tagctgtcat caacagaaat atcaataaga tagggagccg tcgcgctatt ggtatatatc
      181 gccccgccat aaccttcggc agtgttattt gtaaaggcag tgttatttat tatcgtataa
      241 cctggaggat gcttgctgtc gctattatta tcggtaacat cgattgcccc gccatcgcca
      301 tcactgtaac ttgttgatgt atatgcctgg ttgttatcaa aaataacatc acttaaatag
      361 acgtcattat taatggtata aattgcgcca cctttgccat cattagcgat attattgcga
      421 aacatggcgt tagtgacact taaatcgacg gcaccagtat cgttagtacc agaagaatag
      481 attgcgccac catagccgcc tgcgacgtta ccggaaaaaa tgacatcagt aagattcagc
      541 gttgagtttt ctttagcaaa tatggccccg ccgttataat attctcctgt aacggtgtta
      601 ttggcaaaca gagtcattcc agtttcgttt tctggtgata atgtaaattc cgctccttgt
      661 tgcaaaaata cggccccacc gctggcatta ttggtcatat ccgaaaaaac cagccattgc
      721 ccatcggcga tactccagtc ctgcgtaatg cctgaaaggc tttgcctgct ggcgtgacaa
      781 ctcgctttga tatcatatcc ctggcataaa tcaatggccg ccgcgacacc gttagcagag
      841 aaaagagatg caatcattga cgggagtaga gataaatact ccttgcgtag aaagataatc
      901 cgcatattaa tctgaccatc attttatata agacggcata tttaaacgca ttatacaata
      961 tgggttatct ttcttttgta aattgtccaa cccgatgcaa tggctatttt gttattattt
     1021 gatgaataat atcagtgcgt cataattcaa gttaataacc ttaagggata ccagttatat
     1081 ttaaactaaa ttatagtaat gaataatttt cttataatat aaggtaaatt aacaaaatgg
     1141 cttcgcattt aacaataacc gaatagaaaa caaccatttc gacatcaaca atcgcttaca
     1201 ttcgcttata tattgaccac aactgataca tcagattatg tgatgactcg tgcttagatc
     1261 aatttttgca atcattaaca aaaagattaa taaaccatct atatcaattt atccaaccta
     1321 ttctgccgtt caagaaatcg ccgaacagtt atttttaaca aatttttctc ttcccattga
     1381 ctttcccgga caccttgtct gacctaaggt gcgcgaaagc cactttttcc ttcctgagtt
     1441 atccacaaag ttatgcactt gcaagagggt cattttcaca ctatcttgca gtgaatccca
     1501 aacatacccc ctatatatag tgttctaagc agcttccctt actacaggta gtctgcatga
     1561 aactattgcg gaaagaattc caaaaacagg tacgacatac atgaatcaga atctgctggt
     1621 gacaaagcgc gacggtagca cagagcgcat caatctcgac aaaatccatc gcgttctgga
     1681 ttgggcggca gaaggactgc ataacgtttc gatttcccag gtcgagctgc gctcccacat
     1741 tcagttttat gacggtatca agacctctga catccacgaa accattatca aggctgccgc
     1801 agacctgatc tcccgtgatg cgccggatta tcagtatctc gccgcgcgcc tggcgatctt
     1861 ccacctgcgt aaaaaagcct acggccagtt tgagccgcct gcgctgtacg accacgtggt
     1921 gaaaatggtc gagatgggca aatacgataa tcatctgctg gaagactaca cggaagaaga
     1981 gttcaagcag atggacacgt ttatcgatca cgaccgtgat atgaccttct cttatgctgc
     2041 cgttaagcag ctggaaggca aatatctggt acagaaccgc gtgaccggcg aaatctatga
     2101 gagcgcccag ttcctttata ttctggttgc cgcgtgcctg ttctcgaact acccgcgtga
     2161 aacgcgcctg caatatgtga agcgttttta cgacgcggtt tccacattta aaatttcgct
     2221 gccgacgcca atcatgtccg gcgtgcgtac cccgactcgt cagttcagct cctgcgtact
     2281 gatcgagtgc ggtgacagcc tggattccat caacgccacc tccagcgcga ttgttaaata
     2341 cgtttcccag cgtgccggga tcggcatcaa cgccgggcgt attcgtgcgc tgggtagccc
     2401 gattcgcggt ggtgaagcgt tccataccgg ctgcattccg ttctacaaac atttccagac
     2461 agcggtgaaa tcctgctctc agggcggtgt gcgcggcggt gcggcaacgc tgttctaccc
     2521 gatgtggcat ctggaagtgg aaagcctgct ggtgttgaaa aacaaccgtg gtgtggaagg
     2581 caaccgcgtg cgtcatatgg actacggggt acaaatcaac aaactgatgt atacccgtct
     2641 gctgaaaggt gaagatatca ccctgttcag cccgtccgac gtaccggggc tgtacgacgc
     2701 gttcttcgcc gatcaggaag agtttgaacg tctgtatacc aaatatgaga aagacgacag
     2761 catccgcaag cagcgtgtga aagccgttga gctgttctcg ctgatgatgc aggaacgtgc
     2821 gtctaccggt cgtatctata ttcagaacgt tgaccactgc aatacccata gcccgtttga
     2881 tccggccatc gcgccagtgc gtcagtctaa cctgtgcctg gagatagccc tgccgaccaa
     2941 accgctgaac gacgtcaacg acgagaacgg tgaaatcgcg ctgtgtacgc tgtctgcttt
     3001 caacctgggc gcaattaata acctggatga actggaagag ctggcaattc tggcggttcg
     3061 tgcacttgac gcgctgctgg attatcagga ttacccgatc ccggccgcca aacgtggagc
     3121 gatgggtcgt cgtacgctgg gtattggtgt gatcaacttc gcttactacc tggcgaagca
     3181 cggtaaacgc tactccgacg gcagcgccaa caacctgacg cataaaacct tcgaagccat
     3241 tcagtattac ctgctgaaag cctctaatga gctggcgaaa gagcaaggcg cgtgcccgtg
     3301 gtttaacgaa accacttacg cgaaagggat cctgccgatc gatacctata agaaagatct
     3361 ggataccatc gctaatgagc cgctgcatta cgactgggaa gcattgcgtg aatcaatcaa
     3421 aacgcacggc ctgcgtaact ctacgctttc tgccctgatg ccgtccgaga cctcttcgca
     3481 gatctctaac gccactaacg gtattgaacc gccgcgcggt tacgtcagca tcaaagcgtc
     3541 gaaagacggt attttgcgcc aggtggtgcc ggactacgag cacctgcacg acgcctatga
     3601 gctgctgtgg gaaatgccgg gtaacgatgg ctacctgcaa ctggtaggta tcatgcagaa
     3661 atttatcgat cagtcgatct ctgctaacac caactacgat ccgtcacgtt tcccgtcagg
     3721 aaaagtgccg atgcagcagt tgctgaaaga cctgctcacc gcctacaaat tcggggtcaa
     3781 aacgctgtat tatcagaaca cccgtgacgg cgctgaagac gcgcaggacg atctggtacc
     3841 gtcaatccag gacgatggct gcgaaagcgg cgcatgtaag atctgatatt gagatgccgg
     3901 atgcggcgta aacgccttat ccggcctacg gctcggtttg taggcctgat aagacgcgcc
     3961 agcgtcgcat caggctccgg gtgccggatg cagcgtgaac gccttatccg gcctacggct
     4021 cggatttgta ggcctgataa gacgcgccag cgtcgcatca ggcacaggat gcggcgtaaa
     4081 atgccttatc cggcattaaa ctcccaacag gacacactca tggcatatac caccttttca
     4141 cagacgaaaa atgatcagct caaagaaccg atgttctttg gtcagccggt caacgtggct
     4201 cgctacgatc agcaaaaata tgacatcttc gaaaagctga tcgaaaagca gctctctttc
     4261 ttctggcgtc cggaagaagt tgacgtctcc cgcgaccgta tagattacca ggcgctgccg
     4321 gagcacgaaa aacacatctt tatcagcaac ctgaaatatc agacgctgct ggattccatt
     4381 cagggtcgta gcccgaacgt ggcgctattg ccgcttattt ctattccgga actggaaacc
     4441 tgggtcgaaa cctgggcgtt ctcagaaacg attcattccc gttcctatac tcatatcatt
     4501 cgtaatatcg ttaacgatcc gtctgttgtg tttgacgata tcgtcaccaa cgagcagatc
     4561 cagaaacgtg cggaagggat ctccagctat tacgatgaac tgatcgaaat gaccagctac
     4621 tggcaccttc tgggcgaagg cacccacacc gttaacggta aaaccgtaac cgttagcctg
     4681 cgcgagctga agaaaaaact gtatctctgc ctgatgagcg ttaacgcgct ggaagcgatc
     4741 cgcttctacg tcagctttgc ttgttccttt gcctttgcag aacgcgaact gatggaaggt
     4801 aacgccaaga ttattcgcct gattgcccgc gatgaagccc tgcacctgac cggtactcag
     4861 catatgctga atctgctgcg cagcggcgcg gatgatccgg agatggcgga aattgccgaa
     4921 gaatgtaagc aggagtgcta tgacctgttt gtccaggcgg cacagcaaga gaaagactgg
     4981 gcggattatc tgttccgtga cggttcgatg attggtctga ataaagatat tctctgccag
     5041 tacgttgaat acatcaccaa tatccgcatg caggcagtcg gtttggatct gccgttccag
     5101 acgcgctcca acccgatccc gtggatcaac acctggctgg tgtctgataa cgtgcaggtt
     5161 gctccgcagg aagtggaagt cagttcttat ctggtcgggc agattgacgc ggaagtggac
     5221 accgacgatt tgagtaactt ccagctctga tggcccgcgt taccctgcgc atcactggca
     5281 cacaactgct gtgccaggat gaacaccctt cccttctggc ggcgctggaa tcccacaatg
     5341 ttgcggttga gtaccagtgc cgcgaaggtt actgcggctc ctgtcgcacc cgactggtcg
     5401 caggtcaggt ggactggatt gccgaaccgt tagcctttat tcagccgggg gaaattttgc
     5461 cctgttgttg ccgggcaaaa ggcgatattg aaatcgagat gtgaattggt tgtagtgcca
     5521 gatacaacgc ttatacgtct tatctggcct acaacgatta catggcgtag taatacgctt
     5581 tcacctgctc ccagtccgct ttggggattg gctccagata ttttccagct ggcggaagtc
     5641 atgattaatc gctttattgc gacgcaagcg gcgacgactt ttctccaggt caagaaaacc
     5701 agcttctgct ttaccttctg ttttcacata gatatggcga acataacaac agccatgctg
     5761 acgattaacg ctatgcattt tcttaaacgc cagcgccact gctttcaaca tggcttgccg
     5821 tacttcgtca gaatagggcg atactgcatg ccgggcatac cagtcagcaa tgctgatgaa
     5881 ccccgccata tcttcagtca ccagcaacgc gcgccattca ccctcaattt tcaccgcttc
     5941 accaaaaacg atcttcggca cgatgacacc ggcccgttcc agttctttaa ttaccgcaac
     6001 ctcacggaca atcgttggtc ggccgaacgg ataacgtacg gaatgaaaca gatgatgcgt
     6061 catgcgcttt acatacagct ttttgccgtt gcgctcgacg cattgcaccc cgctcatacc
     6121 attacggcga tagttaggct cttcaaccca gtcgccctct gttgcccacc agtgattaaa
     6181 ttcgtcgtac tttgctgaaa ctgccatacc ctatcgcctg tcatttttat taacgacaat
     6241 gactataggt ggttacctga ggaaaatctt aatgaaacgt gtcgtattaa tgaatttctt
     6301 ttgccgctac tagtgtgaca ccgcttttgc ccttcatttg cagcgcatag attccaccag
     6361 ccacgaccac taatagtgcg cccagcagat aaaatgtgaa tttaatcagg tggatacagc
     6421 gatgaatttt attcgacagg gattaggcat cgccttatta ccagagttaa cgctgaaaac
     6481 cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac aga
//
LOCUS       DAJTVE010000187         6512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000187 DAJTVE010000000
VERSION     DAJTVE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..250
                     /locus_tag="OO439_003836"
     CDS             38..250
                     /locus_tag="OO439_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318093.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="OO439_003837"
     CDS             283..501
                     /locus_tag="OO439_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6318094.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="OO439_003838"
     CDS             656..889
                     /locus_tag="OO439_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6318095.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO439_003839"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO439_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6318096.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="OO439_003840"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="OO439_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="OO439_003841"
     CDS             1917..2660
                     /locus_tag="OO439_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6318097.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="OO439_003842"
     CDS             2690..3229
                     /locus_tag="OO439_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6318098.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="OO439_003843"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="OO439_003843"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6318099.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO439_003844"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO439_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6318100.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="OO439_003845"
     CDS             4582..6102
                     /locus_tag="OO439_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318101.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6512)
                     /locus_tag="OO439_003846"
     CDS             complement(6135..>6512)
                     /locus_tag="OO439_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318102.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1712 a         1489 c         1550 g         1761 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gc
//
LOCUS       DAJTVE010000188         6384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000188 DAJTVE010000000
VERSION     DAJTVE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..326
                     /locus_tag="OO439_003847"
     CDS             6..326
                     /locus_tag="OO439_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6318103.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OO439_003848"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OO439_003848"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6318104.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OO439_003849"
     CDS             2213..2929
                     /locus_tag="OO439_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6318105.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3382..3457)
                     /locus_tag="OO439_003850"
     tRNA            complement(3382..3457)
                     /locus_tag="OO439_003850"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Asn,seq:gtt)
     gene            complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO439_003851"
     CDS             complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO439_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6318106.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5206..5281
                     /locus_tag="OO439_003852"
     tRNA            5206..5281
                     /locus_tag="OO439_003852"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5239..5241,aa:Asn,seq:gtt)
     gene            complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OO439_003853"
     CDS             complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OO439_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6318107.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1814 a         1542 c         1372 g         1656 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     3481 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     3541 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg
     3601 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     3661 cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     3721 atgcccagca cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac
     3781 atggcgtaac gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac
     3841 caaataggca taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt
     3901 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     3961 gccgttaatc caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct
     4021 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt
     4081 ccgggtaagt tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg
     4141 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     4201 tcgacactcg cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     4261 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     4321 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat
     4381 cccagtcccg gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
     4441 atattcaggc taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc
     4501 ccgctaccaa taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
     4561 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     4621 tcgccaaaat gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     4681 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
     4741 acgacagtag taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg
     4801 tccgccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
     4861 agaaccccca tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt
     4921 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
     4981 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     5041 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     5101 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg
     5161 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     5221 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5281 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     5341 tcttcactgt tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa
     5401 agttccagaa agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     5461 acagtattag catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     5521 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     5581 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     5641 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     5701 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     5761 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     5821 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     5881 gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     5941 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta
     6001 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     6061 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     6121 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     6181 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     6241 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag atttcccgtt
     6301 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     6361 gatataacgc ttagctcacc aatt
//
LOCUS       DAJTVE010000189         6294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000189 DAJTVE010000000
VERSION     DAJTVE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            143..1318
                     /gene="mdtL"
                     /locus_tag="OO439_003854"
     CDS             143..1318
                     /gene="mdtL"
                     /locus_tag="OO439_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6318108.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1293..2252
                     /gene="yidZ"
                     /locus_tag="OO439_003855"
     CDS             1293..2252
                     /gene="yidZ"
                     /locus_tag="OO439_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6318109.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2409..3158
                     /locus_tag="OO439_003856"
     CDS             2409..3158
                     /locus_tag="OO439_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6318110.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3180..3730
                     /gene="yieF"
                     /locus_tag="OO439_003857"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3180..3730
                     /gene="yieF"
                     /locus_tag="OO439_003857"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3784..5121)
                     /gene="adeP"
                     /locus_tag="OO439_003858"
     CDS             complement(3784..5121)
                     /gene="adeP"
                     /locus_tag="OO439_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6318111.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5287..5952
                     /gene="yieH"
                     /locus_tag="OO439_003859"
     CDS             5287..5952
                     /gene="yieH"
                     /locus_tag="OO439_003859"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6318112.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6019..>6294
                     /locus_tag="OO439_003860"
     CDS             6019..>6294
                     /locus_tag="OO439_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318113.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVV"
BASE COUNT         1550 a         1601 c         1594 g         1549 t
ORIGIN      
        1 aatttggcta aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc
       61 acttatgtcc aaatggcaac tcgcctgatc ctccttcacc acgtatgctt tgcgtcacct
      121 tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt
      181 actttatccc gccgggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
      241 caatgccagc gaagcgcagt tgcatattgc gttctccgta tatctggcgg ggatggcagc
      301 tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaagccgg tcgccatacc
      361 cggcgcggcg ctatttatta ttgcctcggt gttctgttca ctggctgaaa ccagcacgtt
      421 atttcttgca ggccgatttc tacaggggtt gggcgcaggc tgttgttacg tagtggcgtt
      481 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cattactcaa
      541 cggtattacc tgcatcattc cggtgttagc gccagtgctc ggacatctga ttatgcttaa
      601 attcccgtgg cagagtctgt tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt
      661 gtctttgttt attttaaaag aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg
      721 agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct
      781 cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg gtattgctga tggaaatcat
      841 gggttttgag cgcggagaat acgccaccat tatggcgttg accgctggcg tcagcatgac
      901 cgtttcattc tccacgccat ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
      961 ctcgcaggtg ttattccttg cggcggggat cacccttgcc gtttcacctt cccatgcggt
     1021 ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat
     1081 gagtcaggcg ttaggaccat tttcattacg cgcgggcgta gccagctcga ccttaggtat
     1141 tgcgcaggtt tgcggttcgt cactgtggat ttggctggca gcggtggttg gtattggcgc
     1201 atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat
     1261 gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa atccatcacc acgcttgatc
     1321 tcaatctgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
     1381 agcggatgaa cgtgacccct tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt
     1441 ttgacgaccc gctctttgtg aactcaccgc tgggcctgtc gcccacaccg ctaatggtca
     1501 gcatggagca aaatctggcg gaatggatgc aaatgagcaa tctgctgctg gataaaccgc
     1561 accaccagac accgcgcggc ctgaagtttg agttggcggc ggaatcgccg ctgatgatga
     1621 tcatgcttaa tgcgctgtcg aaacggatct accaacgtta cccgcaggcg accatcaaac
     1681 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
     1741 tttccggtcg cgaaagccat cctcgctcgc gggagctgtt aagctcgcta ccgttagcca
     1801 ttgattatga agtgctgttt agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg
     1861 cactgcatga aacgtggaat ctggacacct ttttacgtta tccgcatatc agcatttgct
     1921 gggaacagag cgacacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
     1981 cgattgctat gagtctgccg gaattcgagc agtcactgtt tatggcagcg caacccgaca
     2041 atctgctact ggcgaccgcg ccgcgctact gtcagtacta caatcaactc catcaactgc
     2101 cgttggttgc tcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagttcctt
     2161 ttaccctgct gtggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa
     2221 ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt
     2281 cacaataaaa tgtaaaaaag ttgtaacaag cttgtctgaa tcgaactttt agccgcttta
     2341 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
     2401 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
     2461 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc
     2521 gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
     2581 aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc
     2641 cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg
     2701 aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc
     2761 ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta
     2821 acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg
     2881 ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg
     2941 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg
     3001 cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt agatggcaaa cacagctgga
     3061 aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
     3121 cccagctctc taccgtgaag tctttttcgc caaattgata gacaaccaca ggagtcatta
     3181 tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
     3241 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac
     3301 cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag
     3361 caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc
     3421 cggaatataa ctactctgta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgct
     3481 ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg
     3541 tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga acaagccgga
     3601 atttatgggc ggcgtgattc agaacaaagt tgatcggcaa accggagaag tgattgatca
     3661 gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt
     3721 taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa tgcgctatcg
     3781 attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa cgatgattac
     3841 gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt aggagataaa
     3901 gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg cggtaataaa
     3961 cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg aggtcatcaa
     4021 cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc ccgccagcgg
     4081 cgacagaaag ataaccagca ggaacaacag accaacaacc actgccgtca gaccggtacg
     4141 accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag aagtaccgat
     4201 aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca tgcgcgggaa
     4261 tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg taccggagga
     4321 gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga ggttaaacga
     4381 cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggcgcag aaacgatgcc
     4441 attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga tagaaaccag
     4501 caccgctgcg tgaatgttgc gcgaggccag aatggcgatg atgaagaaac cgaggatccc
     4561 cagcagcacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg ggttagcgac
     4621 aatcacacct gcgtttttca gccccatcat gccaatgaac agaccgatac cgctggtaat
     4681 acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc ggaaaatggt
     4741 caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct gccacggcag
     4801 ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg caggagccag
     4861 cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag caatcagaca
     4921 ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt gtgggttaac
     4981 aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt cagttcgtgc
     5041 cgtagtacca tgttcgcgca gtttaaaaac tcgttccagc atcccctgac cagaagtctg
     5101 ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc cgtcgctatc
     5161 gtataccaaa atgcgacaat aggcgcgttt gtgagagact ttttttattg gatttactta
     5221 tacggcaacg attgcgttgc gcaaatcggc tctacgaaaa cgttaaactg attaaaaagg
     5281 aaaggcatgt cccggataga agcggtattt ttcgactgcg acggtacgct ggtcgacagt
     5341 gaagtcattt gctcccgcgc atatgtaacg atgtttcagg aatttggtat tacgctcgat
     5401 cctgaagagg tattcaaacg tttcaaaggt gtaaaactgt acgaaattat cgatattgtt
     5461 tcccttgaac atggtgttac gttagcgaaa acagaatctg aacacgttta ccgtgcagaa
     5521 gtcgctcggc tgttcgattc agaactggaa gccatcgaag gggctgaagc gctcctgtca
     5581 gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat gcagcattct
     5641 atgggcaagc tgaatatgtt gcactacttc ccggataaac tgttcagcgg ctacgatatt
     5701 cagcgctgga agccagaccc ggcgttaatg ttccatgcgg caaaagcgat gaatgtaaat
     5761 gtagaaaact gcattctggt tgatgactca gttgccggtg cgcaatctgg tatcgacgca
     5821 ggtatggaag tgttctactt ctgcgccgac ccgcacaata agccgatcgt tcacccgaaa
     5881 gtcaccacct ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg tggttgggat
     5941 attacggcat agttcttcac actcccttca cttaccccgc ttaaattggc gctcaaaggt
     6001 aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa tacatcacgg actttatttt
     6061 gcggttttag gaccgttaat tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa
     6121 gaaccgctgg ttcttttggt aataatacag gtacttccat tgtttctctt attgtcgatt
     6181 actacgggag ctattcctgc attgttaacc ggcgtgatgg tcgcctgtct gccggaaaag
     6241 atcgggtcac agaaaaatta tcgttgtctg gctggtggca taggtggcgt cgtt
//
LOCUS       DAJTVE010000190         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000190 DAJTVE010000000
VERSION     DAJTVE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..952
                     /locus_tag="OO439_003861"
     CDS             122..952
                     /locus_tag="OO439_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6318114.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO439_003862"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO439_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6318115.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="OO439_003863"
     CDS             complement(2081..3541)
                     /locus_tag="OO439_003863"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6318116.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO439_003864"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO439_003864"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6318117.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6255
                     /gene="gntP"
                     /locus_tag="OO439_003865"
     CDS             5146..>6255
                     /gene="gntP"
                     /locus_tag="OO439_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6318118.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1587 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgct
//
LOCUS       DAJTVE010000191         6194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000191 DAJTVE010000000
VERSION     DAJTVE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..755
                     /locus_tag="OO439_003866"
                     /pseudo
     CDS             13..755
                     /locus_tag="OO439_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(797..1249)
                     /locus_tag="OO439_003867"
                     /pseudo
     CDS             complement(797..1249)
                     /locus_tag="OO439_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1313..1459
                     /locus_tag="OO439_003868"
                     /pseudo
     CDS             1313..1459
                     /locus_tag="OO439_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1459..2616)
                     /gene="intS"
                     /locus_tag="OO439_003869"
     CDS             complement(1459..2616)
                     /gene="intS"
                     /locus_tag="OO439_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6318119.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2778..2852)
                     /locus_tag="OO439_003870"
     tRNA            complement(2778..2852)
                     /locus_tag="OO439_003870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2817..2819),aa:Arg,seq:cct)
     gene            complement(2928..3860)
                     /locus_tag="OO439_003871"
     CDS             complement(2928..3860)
                     /locus_tag="OO439_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6318120.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4154..4909
                     /gene="mlaA"
                     /locus_tag="OO439_003872"
     CDS             4154..4909
                     /gene="mlaA"
                     /locus_tag="OO439_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6318121.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5091..5426)
                     /locus_tag="OO439_003873"
     CDS             complement(5091..5426)
                     /locus_tag="OO439_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318122.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5384..5776)
                     /locus_tag="OO439_003874"
     CDS             complement(5384..5776)
                     /locus_tag="OO439_003874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318123.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5783..6159)
                     /locus_tag="OO439_003875"
                     /pseudo
     CDS             complement(5783..6159)
                     /locus_tag="OO439_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1543 a         1367 c         1374 g         1910 t
ORIGIN      
        1 tgggtggatg atatggcaac tacatgttca gttatattga ttttggagtc ctttgatgtt
       61 tatttcggaa aagagagtgt gtttctggag agaggttcat ctgtacttgt cgactctagc
      121 tctagagatt ttttcctgac atatcctgaa agagtgatag tggcggattt tggcgctgag
      181 tttattagtc gctatttgaa agctaataac ttaagggata tttctgattg tagggaatat
      241 ctatcttatt taaaaataaa ctttgctgac ttcagtttaa ttaaaggatt aattagttgg
      301 gctaatcact gtgctgaata catagaaatt tttgatgagt ctattgcttt tacatgtctc
      361 tctgcatttt cttctgaaaa acaatttgga gtatttctgt ttggatgttt gaaaagcaca
      421 gggctaaagt taaaacgatt attcatacgg atttatctgc accatggcgt cttaaggata
      481 tatcatcaag attatatctc agcgaaagtt tactaaagat gaaattgaaa gaagaggggg
      541 tatcattcag taagatcata cttgatgaga ggatgcaaat ggctgaatat ttactcagca
      601 ctcgttgtta tcctattagt aaagtagcta aggtctgtgg ttatgccagt gtctcatact
      661 ttacttatgt atttagacgt tattttggtg tttctccaag tcaatactct cagaggagtt
      721 cagaaagtaa aattcttact caccagggaa tctgatcatt gttcttgccc ccttatttcc
      781 agacaggggg tgtatcttaa gttaacgtta cccgctgacg tcgatattct cgcggagagc
      841 gataacccag cgcactgtgc ggatggtttt cattgtaatg ttcgatcgcc actgcaagat
      901 tatgcaatgc cgttcttaca ttcggtttcg gcatgaacgc gatgtagtct tccttcatcg
      961 ttttcacgaa cctttctgcc attccattac tctgaggact gctgattgcc gttgtgcagg
     1021 gctccagatt caactctctg gcgaactgcc gtgtttcatg cgctctatac gctgaaccgt
     1081 tatctgtcag ccactggatg gactgttccg gtactttatc gccaaagcgt ttttctatcg
     1141 ctcctaacat gacatcctgc actgtcgctt tatcgtaacc tcccgtgctt gcggcccagt
     1201 ctatggcttc acgatcgcaa cagtccaggg cgaacgtaac acgcagtttt gaatcgccac
     1261 gggtttaaca gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg
     1321 taagcgttat cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta
     1381 ttctgttgcc agcgttgcaa cacgtctcga tatcactacc cacagccttt atgcctggat
     1441 aaagaagtac ggtccggatt actccacttt ttcatcaagc caatccgccc accactgcat
     1501 catttctctg cgcttatcga gatactgagc atggttgtaa ataccgcgca cagatccgcc
     1561 gttggcatgt gccagctgca cttcaatagc gtcagcaggc cattcgtgct cgttcataat
     1621 cgtgctgaat tcatgcctga atccgtgacc gctttccaga ccttcatagc cgatttgttt
     1681 gatcacaagc aataccgcgt tctcgcagat tggcttcttc ttatcgttgc gcccggcaaa
     1741 aacaaactct gagactggtt tggtgattga gcttagcgta gtgagaagtt caaccacctg
     1801 gtctgacatc gggaccacat gaattttgcg tcccttcatc acactggcgt cgatggtgat
     1861 aatcctgttt tcaaaatcga cgttcttcca tagcatggaa cgaagctctt ttgttcttag
     1921 ggctgtgtag cgtaaaactt tggtcgcaat gagcgatacg atacttcctg aaaatgttgc
     1981 cagtgctttg ttgaatgcag gcatctggtc tgctggaagg aacgggaagt tcttcttgcg
     2041 gtatcctttc atggcgtctg caaggtcagg tgccgggtta tatttagccc ttccggtgac
     2101 aatagcgtaa cgaaaaacct cgccgcatct tctgcgggct ttgttggctc gctccattgc
     2161 accgcgatct tcaaatctgc ggattacttc cagcagttgc atcggctcaa tatcctgaat
     2221 ctcaagaccg ccgatgatgg gtaaaatgtc gtcatcaaac attttggcaa gttcagttgc
     2281 atagcctact gaccagactt gcttcttgtg ctcgtaccat tccttgtaaa tcgcactaaa
     2341 tgaattgttg ttagacgaag cctttttcgc ctttaccgga acgatgccaa ccgagatgtc
     2401 tttcctcgca gtccatgcct tatcccttgc ttcctgcaaa gtcataagcg gatattttcc
     2461 gacggtcagg attttctcct taccgtcaat cttgtagcga agctgccata cctttttccc
     2521 ggatacagga acataaaggt acaggccatt accatcgaga aggcggtatg gtttttcttt
     2581 cggctttgct gcttcaatct gcttaacggt gagcatgggt aaaaatccgg tgggtaaaat
     2641 tattttatcc actttttacc cgtcatggag tgcggctgtc aacgatctga cgcgaaccat
     2701 gacgaaccgt gaatatacgg aaggcttgat attcagggga ttttgcggac tggtacggat
     2761 gggagcgaac tgataaatgg tgtcccctgc aggaatcgaa cctgcaatta gcccttagga
     2821 ggggctcgtt atatccattt aactaagagg acaatgcggc atgagtatac ccgctaatgg
     2881 agtgcggggt aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgccgggttt
     2941 ttatcatttt tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc
     3001 ttgttgctca tgtcgttacg aatctgtgca tgactcatta acgcgaagat aaaggtgccg
     3061 ccgcagatgt tccccgctaa agtaggtagt gcgaagggcc agatgaaatc gctccagtgc
     3121 agcgtaccgt taaacaccag atagaggatt tcaacagaac cgaccacgat atgggtggtg
     3181 tcacctaggg caataagcca ggtcatcaat ataatcacca caatctttgc cgcacctgct
     3241 gcaggaaaca tccataccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac
     3301 atctcgctgg gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt
     3361 tcttcattga agataggcat atattcaaat gcccacgcag caataccagt cccgagaata
     3421 ttacccagca gcacgactcc ccataaccgc ataagtaagc caacgttgct cattgtcggt
     3481 ttttgcatga cgggtagtac cgcagtcacg gtgttttcgg taaataattg ctggcgggcc
     3541 attatgacga taataaaacc aaaggtataa ccgagattct ccagtaagaa gctgcccggc
     3601 acaccttcca gttcgacatg aaatatccct tttgccagta acgaagcgcc catcgacaga
     3661 cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc gttccagctc tttttcacca
     3721 tcctggcgga tatgctcatg aattgccatc gcccgggagg ggagtcggtc ttcatctatt
     3781 tctatttttt tgccgcgctc tttttcttcg ctctcaactt caatttcgtc gctgtgttga
     3841 tcaattttgt cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag
     3901 cggctttttt gctcgcaact ggcgggagtt actctgaaaa tgtagaaaag gctgcgtttg
     3961 cctttttctg tttctataga atcaagtagc ctacagggcg gcgattacca ggctatgatc
     4021 aaatcagcaa atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct
     4081 aaacaggcgg atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa
     4141 cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt
     4201 ggggtgtgcg agttccggta cagatcagca agggcgttct gacccgttag aagggttcaa
     4261 ccgcaccatg tacaacttca acttcaacgt attagacccg tatattgttc gaccggtcgc
     4321 tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaat ggtttgagca actttactgg
     4381 caaccttgaa gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg
     4441 gatggtccac tttacccgct ttttcctgaa caccattttg gggatgggcg gctttattga
     4501 tgttgcaggg atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac
     4561 gcttggtcat tatggcgtgg gttatgggcc ttacgttcag ttaccgttct acggtagctt
     4621 cacgctgcgt gatgacggtg gtgatatggc ggatggtctt tacccggttc tttcctggct
     4681 gacctggccg atgtctgtgg gtaaatggac gcttgaaggg atcgaaaccc gcgctcagct
     4741 gctggattcc gatggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc
     4801 gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc
     4861 gaacgcacaa gcgattcagg atgatttaaa agatattgat tctgaataag aaacaaataa
     4921 aaaaggtgag tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa
     4981 ggagataaac cgacaatgac ccggacgtaa tgatgtaaaa atactgatgt tcgtcactga
     5041 ctatttccga tattcaggcg ttgaaaaatc aaaaaactgg aaaaataatt ttaaatatca
     5101 tcaaaattaa cacctggcgc agatagttga ttaatttcat tttgatattt cgcctggcga
     5161 ttttgaagtc ttttttcaac gttatcttcg agatccttaa agtctttgta tttattttca
     5221 ggatggtcaa cgtcaacttt gttagcgaat aaattagaaa ttatttcttt tacattgtcg
     5281 cgtctaaatt ttgcatcgtc cacttgaaaa ttatatctta ttgctcgacc taaacgttta
     5341 aaaacttcgt taggttctct cagtctttcc ccgttgagct cgtttacaac ctgatgatcg
     5401 gaacctgttg caaatgattc tgccatgata gctttactgc tttcaaacca cgctgaagca
     5461 ttgctgaagc attcctggct ttttcactac aatattgggt gagactatca ccattaatac
     5521 gaattcctct ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat
     5581 ttccgttttt atttttaatt acaatagcga gagagttgcc ttttttatct ttaccgtacc
     5641 aggtaaataa gtccgttggt tcgctattcc cgctctcatc aaattctgtt accgtatgtc
     5701 tgagtttgtg tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta
     5761 atgcgctgaa gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat
     5821 taatgatagt acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt
     5881 ggttgtcgat accaatagta atatggtctg tatcattgga gccaatatca atataagtaa
     5941 ctgaggaatt attaatatta aatcttaagg atcgtcgttt actcgtatgg cgttttttat
     6001 aagctcaagt agatgtttaa tacaggtgct tattccttcc ttaccattaa aaaggttttt
     6061 gagctcttgt actaatccat taacttcatc attgttattt tcattaatgg attctgggta
     6121 agttgcgctg gtattgttga ttgaaataga tggtagcatg attttattcc attttgattg
     6181 atttgttaca aata
//
LOCUS       DAJTVE010000192         5975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000192 DAJTVE010000000
VERSION     DAJTVE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO439_003876"
     CDS             complement(<1..202)
                     /locus_tag="OO439_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6318124.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OO439_003877"
     CDS             718..1287
                     /locus_tag="OO439_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318125.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2015..2188
                     /locus_tag="OO439_003878"
     CDS             2015..2188
                     /locus_tag="OO439_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6318126.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO439_003879"
     CDS             complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO439_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6318127.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO439_003880"
     CDS             complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO439_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6318128.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4300..4890)
                     /locus_tag="OO439_003881"
     CDS             complement(4300..4890)
                     /locus_tag="OO439_003881"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6318129.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO439_003882"
     CDS             complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO439_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6318130.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5708..>5975)
                     /gene="rutC"
                     /locus_tag="OO439_003883"
     CDS             complement(5708..>5975)
                     /gene="rutC"
                     /locus_tag="OO439_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6318131.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1607 a         1537 c         1446 g         1385 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc ccctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     1321 atggggactt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     1381 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1441 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     1501 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     1561 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     1621 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     1681 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     1741 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     1801 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     1861 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     1921 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
     1981 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
     2041 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
     2101 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
     2161 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
     2221 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
     2281 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
     2341 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
     2401 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
     2461 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
     2521 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
     2581 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
     2641 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
     2701 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
     2761 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
     2821 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
     2881 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
     2941 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
     3001 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
     3061 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
     3121 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
     3181 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
     3241 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
     3301 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
     3361 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
     3421 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
     3481 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
     3541 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
     3601 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
     3661 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
     3721 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
     3781 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
     3841 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
     3901 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
     3961 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
     4021 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
     4081 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
     4141 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
     4201 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
     4261 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
     4321 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
     4381 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
     4441 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
     4501 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
     4561 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
     4621 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
     4681 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
     4741 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
     4801 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
     4861 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
     4921 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
     4981 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
     5041 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
     5101 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
     5161 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
     5221 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
     5281 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
     5341 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
     5401 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
     5461 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
     5521 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
     5581 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
     5641 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
     5701 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
     5761 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
     5821 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
     5881 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
     5941 tttgcgcctt tgggtcatcg gcaaacagca cgtta
//
LOCUS       DAJTVE010000193         5934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000193 DAJTVE010000000
VERSION     DAJTVE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            292..368
                     /locus_tag="OO439_003884"
     tRNA            292..368
                     /locus_tag="OO439_003884"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO439_003885"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO439_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6318132.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OO439_003886"
     CDS             complement(1357..2247)
                     /locus_tag="OO439_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6318133.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO439_003887"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO439_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6318134.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRQDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5934)
                     /gene="nfrB"
                     /locus_tag="OO439_003888"
     CDS             complement(5207..>5934)
                     /gene="nfrB"
                     /locus_tag="OO439_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCR6318135.1"
                     /translation="LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESI
                     DAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVR
                     YFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFA
                     EILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQ
                     SLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1559 c         1551 g         1478 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatcctggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agtt
//
LOCUS       DAJTVE010000194         5932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000194 DAJTVE010000000
VERSION     DAJTVE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..225
                     /locus_tag="OO439_003889"
     CDS             <1..225
                     /locus_tag="OO439_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6318136.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            271..621
                     /gene="traA"
                     /locus_tag="OO439_003890"
                     /pseudo
     CDS             271..621
                     /gene="traA"
                     /locus_tag="OO439_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            636..947
                     /gene="traL"
                     /locus_tag="OO439_003891"
     CDS             636..947
                     /gene="traL"
                     /locus_tag="OO439_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6318137.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            969..1151
                     /locus_tag="OO439_003892"
                     /pseudo
     CDS             969..1151
                     /locus_tag="OO439_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1153..2403
                     /locus_tag="OO439_003893"
                     /pseudo
     CDS             1153..2403
                     /locus_tag="OO439_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2400..2786
                     /gene="trbI"
                     /locus_tag="OO439_003894"
     CDS             2400..2786
                     /gene="trbI"
                     /locus_tag="OO439_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6318138.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2783..3094
                     /locus_tag="OO439_003895"
                     /pseudo
     CDS             2783..3094
                     /locus_tag="OO439_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3089..4087
                     /locus_tag="OO439_003896"
                     /pseudo
     CDS             3089..4087
                     /locus_tag="OO439_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4107..4854
                     /gene="traX"
                     /locus_tag="OO439_003897"
                     /pseudo
     CDS             4107..4854
                     /gene="traX"
                     /locus_tag="OO439_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4909..5469
                     /locus_tag="OO439_003898"
     CDS             4909..5469
                     /locus_tag="OO439_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6318139.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5599..5811
                     /locus_tag="OO439_003899"
     CDS             5599..5811
                     /locus_tag="OO439_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6318140.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1401 a         1458 c         1819 g         1254 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcagcg ttaagtgttc agggtacttc tgcgcccgtc
      301 aaaaagaaat cgtttttttc taaattcact cgtctgaata tgcttcgcct ggttcgtgca
      361 gtgatcccgg ttgctgttct gatgatgttg ttccctgagt tagctatggc tgctggtaaa
      421 ggcacggacc tgatggctaa aggtcaggaa acggttaagg cgaccttcgg taaggactcc
      481 agtatcgtta aatgggttgt tctggctgaa gttctggtcg gtgcagtcat gtacatgatg
      541 accaaaaacg tcaagttcct ggtcggcttt gccatactct ctgtatttat tgcagtaggt
      601 atgtctgttg ccggattctg acaggaaata aaacgatgtt gggagacgag aataaactta
      661 agaaatatcg tttcccggaa acactgacca accagagccg ctggtttggc ctgccactgg
      721 atgaactgat ccccgcagca atctgtgttg gctggggtat cacaacatcg aaatatctgt
      781 tcggtattgg tgcagcggtt ctggtttatt tcgggattaa aaaactgaaa aaagggcggg
      841 gcagttcctg gttacgtgac ctgatttact ggtatatgcc aacagccctg ctgcgcggta
      901 tttttcataa tgttcccgat tcgtgtttcc ggcagtggat taaatagaac tgataccagg
      961 attgttatat ggaacacggt gcccgtttaa gtaccagtcg tgtaatggcc atcgccttta
     1021 tatttatgtc agtgcttatt gttctcagcc tctctgttaa cctcattcag ggggtgaata
     1081 actaccgtct tcagaatgag caacgcactg ccgtgacccc aatgggattt aatgtgccct
     1141 ttgccgtgtc acacctaccg taaccagctg gcgtttatcg atattttctt ccggggaatg
     1201 aataacacca actacaacat ggcggtctgt ggcacctccg gggccggtaa aaccgggctg
     1261 atacagccac ttatccgcag cgtgctggac tccgggggct ttgccgtggt gttcgacatg
     1321 ggggatggct acaagtccct gtgtgagaac atgggtggcg tatatctgga cggtgaaacc
     1381 ctgcgtttta acccgtttgc gaacatcacc gatatcgacc agtcagcgga gcgtgtccgt
     1441 gaccagttgt cagtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg
     1501 ctgctgcagg cggtcagggc ctcctggctg gccaaagaga acagggcacg tattgatgac
     1561 gtggtggatt tcctgaaaaa cgccagtgac agcgagcagt atgccgggtc accgactatc
     1621 cgcagccgtc tggacgaaat gattgtgctg cttgaccagt acactgccaa cggcacttac
     1681 ggccagtatt ttaactctga tgagccgtcc ctgcgggatg acgcaaaaat ggtggtgctg
     1741 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt ctccctgatt
     1801 atctacatcg agaacaggat gtaccgcacg ccgcgtaacc tcaagaaact gaacgtcatt
     1861 gatgaaggct ggcgtctgct ggacttcaaa aatcacaagg tcggtgagtt tattgagaaa
     1921 ggttaccgta ccgcccgtcg tcataccggt gcttatatca ccatcacaca gaacatcgtc
     1981 gactttgact ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac
     2041 aaaattatcc tcaaacagag tgcgaaggag ttcgcaaaat acaaccagct gtatccggac
     2101 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
     2161 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgcct gtttgtggac
     2221 ccgttaagcc gcgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
     2281 cgtaaagagg ggctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg
     2341 ccggagatgg cttcgctgga agcctggctg gaagaacatg agaaatacag gagtgttgca
     2401 tgaccacaac gcaaaaaacg actgatgtta cagccccccg acgcagccac tggtggtgga
     2461 cggtgccggg atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca
     2521 gactgaacgc accgatgact gccgccttca acatgaaaca gactgtggat gcattttttg
     2581 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
     2641 acacggcact ggaagccagc ctgcaggcgt ggcagcagaa acaccacgcg gtcattctgg
     2701 tgtcgcctgc cgtggtacag ggggctccgg atatcacccg ggaaatccag caggatattg
     2761 cccggcgaat gagagcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     2821 gcagagtgtg gccgccgccg atcttggcac ctggggtgat ctgtggccgg taaaggagcc
     2881 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     2941 ccggaaaatg gatgccttta aggagcgggt gatccgcaac agtctgcggc cccctgccgt
     3001 gcccggtatc ggacgcacgg agaaatacag cagccggttg tttgacccgt ccgtcagact
     3061 ggctgcggat atccgggata acgaagggcg ggtggccggc tttgagtcag cctacgtggc
     3121 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     3181 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     3241 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     3301 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     3361 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     3421 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     3481 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     3541 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     3601 ccgtgataat cctgacagtg gtgtggtggt aagcattgcc ggtgaaggcc gtccgtggaa
     3661 tcccggtgcc atcaccgatg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     3721 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     3781 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     3841 caaacctgac ctgccggacg gcaaaacaga gctggctgtc agggaaattg ccgggcagga
     3901 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tgctgcgtga
     3961 atcacaacgg gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg
     4021 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     4081 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     4141 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     4201 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     4261 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     4321 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     4381 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     4441 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     4501 cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgttg ggggcctttg
     4561 tccggcacca gttacggcat tgccgggctg ctgatgctgg cagtcagcca ccgactgtac
     4621 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     4681 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     4741 accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc gtttctggcc cggggatttt
     4801 ttcccggtat tctacgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     4861 tggtatcttt gaccgtaaca ggatgattcg tcagaggcag aacacagcat gacagagcag
     4921 aagcgaccgg tactgacact gaagcggaaa acggaagggg agacgcctgt ccgcagccgg
     4981 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     5041 gagaaagcag cccgggaagc agagctggcg gcaaaaaaag cgcaggccag acaggcactg
     5101 tccatttatc tgaacctgcc cacgctggat gaggccgtga ataccctgaa accctggtgg
     5161 ccgggattat ttgacggtga caccccccgg cttctggcct gcggtatccg ggacgtgtta
     5221 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     5281 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     5341 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcggcagcg
     5401 cgtctggata aaatccgccg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     5461 gagaaataaa aaaaagcctc catcagagag gaggcaggga aataaggctt aaaaggaaat
     5521 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     5581 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     5641 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     5701 gcggcagaag catggcgacg ggctgcctgt gttgcgccca ggacagactg gcagcagttt
     5761 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     5821 cagccttcat tactccagga gtcagaaggc tgaaaggagt ttatgagatt tgcttttttt
     5881 aatatccggt cgggcagtat gaccggacag ttctgagtag cacagagttt tt
//
LOCUS       DAJTVE010000195         5845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000195 DAJTVE010000000
VERSION     DAJTVE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1348)
                     /gene="istA"
                     /locus_tag="OO439_003900"
     CDS             complement(<1..1348)
                     /gene="istA"
                     /locus_tag="OO439_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6318141.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
     gene            1521..2684
                     /locus_tag="OO439_003901"
     CDS             1521..2684
                     /locus_tag="OO439_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6318142.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(3144..3581)
                     /locus_tag="OO439_003902"
     CDS             complement(3144..3581)
                     /locus_tag="OO439_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318143.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(3670..>5845)
                     /locus_tag="OO439_003903"
     CDS             complement(3670..>5845)
                     /locus_tag="OO439_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCR6318144.1"
                     /translation="GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKG
                     ETAERWRYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHE
                     TRHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSF
                     GRYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     LASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKN
                     NPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLN
                     AFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT         1306 a         1447 c         1519 g         1573 t
ORIGIN      
        1 cgtatttttt ctgaagtgcc agcagaccca ggactgcacg gtaagccagc tccggatggg
       61 gtttggcttt cagcatggat tcaaccaccc ggcctgtgga cggacctgta cggtttccct
      121 gctcgataag gcgttccggt gtccatgtcc catgccgccg gtggcgttct ggcatgtgaa
      181 gcggattggt tgtgtgttta tattcatgtg tgctgcgcgg atgttgcgcc acacactgcc
      241 ccttgtgcca gagctgtacc accgactgcc ccaccttcag tgacagacgc tgaccgacca
      301 gttcgtgtgg aacggagtac cagtgacggg catattcaac gtggtagtca gggccaacct
      361 tcacggcttt gtattccgtg tattcataag gctccagagg cagcggagag agtgctggtg
      421 catccagcat gcggaagcgt tctgcgcggg tttgattacc gtatcccttc attggtcggt
      481 tattcatgtc cgtcagcaac tcccgaaggc gtgcattcag cgccctgagc gtgtggaagg
      541 tttcattacg gatgcgggca agaagccatc gctcgacgat gaggacgccg ttttctgctt
      601 ttggtttatc tttaggtttg cgcgggcggg ccgggataat aaccgtgcca tagtgctcag
      661 ccagcgcctg ataactgtcg ttgatgacag gctcatagcg atcggctttt ttcactgcgc
      721 ttctgaggtt atcggggatg agcagcttcg gtacgccgcc caggaaggtc aggcaacggc
      781 tgtgggcatt taaccatgac atcatgtcct gaccttcgca cgcttcaaca taggtatagt
      841 tggacgcccc cattacagcc acgaagattg ccacgcggcg tatctctccg gtgtccggat
      901 taataacagg gaccgtagga ccacagaagt cgataaagag cttttcgcca gcacgatgct
      961 cctgtcgcat ggagaggcgt cgcgtttttt tccacttccg gtagcaacgg cagaagtgtg
     1021 agtatcccat cgctttgtca cccgcctgtg ccttgtattc catccacaac agttggcgcg
     1081 tcacgccagg ttttctcatt tccgtatcga agtacagcat gtcaggcata acaagctctg
     1141 acgcagaggt gtctttgggc gggaaaatga gtttctccag cgtgtcatgg gatatatccg
     1201 caggtaatgg ccatgacaga gaagaggctt tgaagcgggc aagaacctca aaaacggttg
     1261 aggcgctgat gtgtaacgtc agtccgatac aacggttagt cagcttctta tcgaacttga
     1321 gctgaaggat cttaaaacaa gtttccatag tgctcctgga ttttctcttt ttggacattg
     1381 acgtctccac tggtttaaac acggcaatgg agactgtggt gaaaagagtt aattcccgga
     1441 gtgactggcg aattccaatc aatgatcgga agtatgccaa atcgtgatcg attgtgatgc
     1501 caaactgtga tcggagatgg atgccaaatc atgatcgatt cgaatgccaa actgcgatcg
     1561 attcagatgc cattttgcag ctggcgtggg cggtctttaa aatctttcgt attccggtga
     1621 atcagtggac gctggcgacg gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt
     1681 tgttgatgaa ctacaaccac ccttacactt ttaccgcgca aaaggcagtg atagcgatcc
     1741 ctatcacgcc acaggtgacg ggaattgtta ctgaagtcac tgacaagaat aatcagctta
     1801 ttcaaaaggg cgaggtgctt tttaagctcg acccggttcg ttaccaggcg cgagttgaca
     1861 gacttcaggc tgacctgatg acggcgacgc ataatataaa gacgctgcgt gcgcagctca
     1921 ctgaagcgca ggccaacacc acccaggttt cagcggagcg cgaccgtctg tttaaaaatt
     1981 atcaacgtta cctgaaaggc agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg
     2041 acgatgcgcg gcaaaatttc ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc
     2101 aggcgcagat ccagagccag ctcgacagta tggttaacgg cgagcaatcg cagattgtga
     2161 gcttaagagc gcaacttact gaagcaaaat ataaccttga gcagactgtc attcgcgcgc
     2221 cgagcaatgg ctacgttact caggtactga tccgcccagg tacatacgca gctgccttgc
     2281 cgctgcgtcc ggtgatggtc ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc
     2341 ggcaaaactc gctgttacgt ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc
     2401 tacctgggca ggtgttccac ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt
     2461 cttatcaggc gcagggggta ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc
     2521 tgggaaccat tgaactggac cctaacgatg atatcgatgc cttacccgac ggcatttacg
     2581 cccaggtagc agtttactcc gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc
     2641 taagaatgac cagttggatg cattatttgt atttggatca ttgaggggga agggggagat
     2701 tatggaactt tgctccctgc aagggctaat tatttttgca tattacttca ttggctgcga
     2761 tgatttttct ttaatattca ttatgttaat aagttctgga tgagtgtgct ctcattggat
     2821 tttttatccg tcttgttctg ccgcaggatg agactataca tcgtcgagat cgcaatgatg
     2881 atatgagttg ttcctcctgc gacggctcta atcaaaaaag agcaggagtg cttttccatc
     2941 tattgcgatg atggctttat tatctgaagg atgacagtca catttaaaaa atatttaatc
     3001 tatagatcac aatgtagggt catggaatat atgtatacaa ctattttctt cttcactttt
     3061 aattatcgta ttcattataa aactaagtta aaaaaactcc tagagagtga gttattaata
     3121 tatttttaaa taaaattaag ttatcattca tcgatgcatt ttatagaatc atcaatggcc
     3181 ataatagtac tattaactct caattttata tttttatcat cactacaaat attaactgaa
     3241 tttatatcaa tgattttttt attcttatct tttattttat ttactgtttc aatgcaatca
     3301 gcggcaagtt ggagtggatt tagatttttc agatatggtc ttatgatctt tcccttactt
     3361 aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct cattatatta
     3421 gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca
     3481 gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta
     3541 ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat
     3601 tgtcttgcag taactgcact cgcagaaata gagaaaaaaa tacaattatt aataactttg
     3661 ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt
     3721 taatttttgc atgtcattag atgaaccagt tatacaaccc tcggaacaga atcgaggtcc
     3781 taaagacgga ccgaaagcgt ttaaacaact ttgtgttaaa aatccatcac gattatatgt
     3841 ttctgtattt gctgacggta ctaaacgtat accattaggt tttctattgg ttgctccagg
     3901 accattaaca ttccagctcc ataccccttg aggtattgga cctctatttt gtaagtgtgt
     3961 acaaccagga ttatttttac attgcatatt attaccatta ttaccagagg cgacaggaat
     4021 agtaagcaca tcataagagt tatcatcatt ttgcgtatct gaaacgcatg aaagcatact
     4081 taaagttatg ctgtataaac aggtcgctaa tcccaacggg tcaatatcag ttatcggatt
     4141 caacggatac tgataaaaat tccatccccc cttcagtcca atcggatcct gagtgatata
     4201 tcgcccctgc agcgggtcat aatagcggtg gcggttgtaa tacaggccgg actcctcatc
     4261 atactgctgc cccggcaggc ggataagctg ctgcagctga tgcgggttct cttcattcag
     4321 caggttgccc cactcatcgt attctgcgca ccattctgtt gccccttccg tgctgataag
     4381 caccagcggc aggccgcgat ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta
     4441 caccgggtcc atctggtttt gcatctgctc caccgtcagg ccgcacgatg ccagccagcg
     4501 gcggctttcc tcactcaccc ggtcagccag gatttcactt tccagccggt cgagcatctg
     4561 caccagcacc ggcgggaaca ccacactgcc accgtcttcg ccgccggact gctgaagggc
     4621 atccgccagg ctgcggcgct gcgttttcgc cagctcaccg gtggcagttt cgaccctgat
     4681 gagtggcgtg aagctccccg gctgataaat cgtctggatg cgggttctgt cgttctgtat
     4741 tgtggtcagc cggtcgccgt cccagccgta ccaggtcact tgcggtttcc gtgacagcga
     4801 catccagccc gtcaggtccc gttcacgccg ccatacccgt tttgccaccc tgcggcccag
     4861 cgggtcgtaa agatagcgac tttcgaccag cggctctgca tattgtgtcc gcgtgtagtg
     4921 caccagccgg tgctgactgt cgtaatggta ccggtgggtg cgctcatcat ccgtgcggat
     4981 aaccccttcc gggatgaggt cggttttctc tgtcagtctg ccgtgatggt cataccggta
     5041 aagatagtgc gcgtcacggg cgatacggtt atccggccac atgctgaggg tgctgtccgg
     5101 gtgcagctcc gggtcgggca ggcggttccc cgccgggtct gtggcatacg ggatgcggat
     5161 atccagattc gctgcggtgg tgtgaacgcc ggtcagcctg ccggtggtgc tgtagctgta
     5221 actccgggtc tggcgcgggc tgctgatgcg gatgagttcg ccgttgtcgt tccaggtgta
     5281 atcgcggtca gacagcaggc tgttcaggtg ctggctctgt aactgcccgg caggggtata
     5341 agcgatggtg agttcataac ggccgaagct gcgcagcgtt tcccggtgca ggcggtcgcg
     5401 ggtgtactcc accagcggtg tgtcgccgag tttcatgcct gacagccagc cgctgccgta
     5461 ggtcagccat tccacggcgg acaggctgtc cggtatacag cggttcgcca gcccctgcgc
     5521 gttgtacgca tgtctggtct catgctgcca gagcagtgct tccgtctgcg ggtgatgcac
     5581 cgtctgacgc tcaccggtca gccggccttt ctcatcatac ccgtaatgca ccgtcacccg
     5641 gtgcccttcg ctgagatggc tgatgtctgt cagccagccg cgttcgtcgt accgccaccg
     5701 ctctgcggtt tcacccttca cggtgcggtg cgtgaggcgg tctgcttcgt catagtgcca
     5761 gtgggtgacc agcccctcat cctcgctgcg gataagtttg ccggtcaggt cgtggtgata
     5821 acgctgtgtg cggccgtcaa agccg
//
LOCUS       DAJTVE010000196         5836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000196 DAJTVE010000000
VERSION     DAJTVE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO439_003904"
     CDS             complement(<1..242)
                     /locus_tag="OO439_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6318145.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OO439_003905"
     CDS             complement(604..3387)
                     /locus_tag="OO439_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6318146.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OO439_003906"
     CDS             complement(3444..5816)
                     /locus_tag="OO439_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6318147.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1487 a         1320 c         1312 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttgcgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAJTVE010000197         5796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000197 DAJTVE010000000
VERSION     DAJTVE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(84..755)
                     /locus_tag="OO439_003907"
     CDS             complement(84..755)
                     /locus_tag="OO439_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6318148.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OO439_003908"
     CDS             complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OO439_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6318149.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OO439_003909"
     CDS             complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OO439_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6318150.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2484..2897
                     /locus_tag="OO439_003910"
     CDS             2484..2897
                     /locus_tag="OO439_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6318151.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OO439_003911"
     CDS             complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OO439_003911"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6318152.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OO439_003912"
     CDS             complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OO439_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6318153.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(4861..5271)
                     /gene="fliS"
                     /locus_tag="OO439_003913"
     CDS             complement(4861..5271)
                     /gene="fliS"
                     /locus_tag="OO439_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6318154.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5286..>5796)
                     /gene="fliD"
                     /locus_tag="OO439_003914"
     CDS             complement(5286..>5796)
                     /gene="fliD"
                     /locus_tag="OO439_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6318155.1"
                     /translation="DPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFKTLN
                     EIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGYLAD
                     DGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTSTYLS
                     QQFTAMSNS"
BASE COUNT         1423 a         1529 c         1428 g         1416 t
ORIGIN      
        1 cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcct
       61 cgtccttcag ggcgtggagg atgtcaacaa acaggttgaa ttaactccgc gccctggttt
      121 ttgacattac ctaccgcgcg cgataccggg tgccaggtga actggtttgc tggcacacag
      181 ccgttggtag caatttctga tgcttcttta ccgccaatct cttgccgcat ccattcctgt
      241 gcagcttctg gcgacagaac cagcgggcgg cggtcatgaa tgtctaccag accctggtct
      301 gctgcagcag tgacaattaa aaatccttcg gcttcgtcac cacgctcaaa tggtgtgcta
      361 cctatcgcgg ccataaaaat aggttgtcca tcagcacgat agataaaaaa aggctgtttt
      421 ttgtcgcctt cttttttcca ctcaaaccag ccatcggcaa aacagattgc ccgaccatgt
      481 tgccagagcg gtttaaacat gcgactggtt gcggcagttt ctacgcgggc gttaatcagc
      541 ggcggtttat cccaccatcc gggagcatat ccccagaaaa ccggatccag atgaaggtgt
      601 tcatcacgtt cactgagcag aagaactttg gttcccggcg cgacgttgta tctgccaatg
      661 ggttcgggat cgtagggaat atcgcgttca atatcttccg cgagaagcgc aaggtaatct
      721 tcacgcgttt gggattgggc aaagcgtcca cacatagaaa cctcctgaga tcatactgaa
      781 aagaatagga gagggggcgg gtaaaatcta tgtgtcagag gcaacaatgt tcccgctgtg
      841 ggccacagcg ggaatgaggg attacttctg aattaaataa cgaatggtcg gcccgtcttg
      901 ctgaatatcc agcaccgtat agccgtgatt acgcgcatcc agtggaatat tattgatcga
      961 ctgtggacag tcgctcacca cttccaggat ttcccctttt tttaactgcg gcatcgcctc
     1021 aagggttgcg actgccggat aagggcaggg ttcacccacc atatcaaggc ggtaatcagg
     1081 aacgatattt ttcatgcgat ctccttagca gtctgcggtg ccgcacggcg gaagaagcgt
     1141 ttttcccagc cgataatcaa catcagcgca gtaaacaaca gcaaatatgt caccagcagg
     1201 ccacccatcg ggccaaaggt tttcagcagg ttgattttgt cccagtcggt ggccagcgcc
     1261 ggagcgaaat catcccagta atacgccaga atcgtcgagc cgatcacatt gcccagaccg
     1321 acccaccagt agtgcacctg gccttctacc gcgcggtaca tccagccggt ttcgcagccg
     1381 cctgccagca cgatgccaaa accaaacagt aaaccaccaa ttaccgcgtt tggtcccgcc
     1441 cacatgattt tgggttcaac gcctaactgt acgtaactga agatcccgat ggcactcacc
     1501 gccataccga taatgattgc tttcgccata tgggtacgtc cggtgatcca catatcgcgg
     1561 aacgccgaag taaagcagat ttgcgcacgt tcaatcagta aaccaaagcc gacgccaaac
     1621 agcattgcca gccccagttt gggttggttc atcgccgtga gcagcgccca gcccagcatg
     1681 ccgaaaaaga ccagcatccc gagacgaaaa cgacgccgcg cctgatccgg tttttgcgtc
     1741 agcggtgagg cggcagaaac tttctgcatt ttcacgggaa tacggaagat gggcagcagg
     1801 gtaaagcgcg cgccaaacca tgaaccaatg gcagtggcga tggcaaagaa ccaggcatgc
     1861 agcgagaact gaggaatgcc ggtaaagaac gccgccaggt tacagcccat tgccagacgc
     1921 gcgccaaaac cggcgataat gccgccaata atggcctgca taatgcggat acggctgcgc
     1981 ggcatgcgca gtttgacatt gttggcccac agcgctgcgg caaagcagcc gccaaacatg
     2041 ccgaggatca tcatcccgtc gatgcgggtt aatggcgatc cttccagatg gataatttta
     2101 aaataacccc actcttcagc atggacgcca aacagctgca ggagctgacc gccccaacgg
     2161 gtaaattcac ccgtgacagc ccaaaaggtg ccagtaatgc caaaatagta agtggagaga
     2221 atacccgccg cgatgaccgc agggatgggt gcccagaatt taatcaacca ggcgtgtttg
     2281 aattgctgcc atgacatgaa tgtagcctct gaactcagaa ggtttgaaac aagaatcgcg
     2341 aatcatacat catgcaaatt tattttcggg gtaataattc acaaaaataa tagttagatc
     2401 aaatttctct ctgaacctgg aaaggctggc gacatcaggg ctttaatgcc acaatcctct
     2461 tttcttctca tgcaggacga atgatgaaaa aactcgcaat tgcaggtgcg ctcatgttgc
     2521 tggcgggctg tgccgaagtc gaaaactata acaatgtcgt gaaaaccccc gcgccggact
     2581 ggctggcggg ctactggcag accaaagggc cgcaacgtgc gctggtcagt ccggaagaga
     2641 taggcagcct gattgtcact aaagagggcg ataccctgga ttgccgtcag tggcagcggg
     2701 tgattgccgt gccgggcaag ttgacattga tgtctgatga tctcactaac gttaccgtaa
     2761 aacgtgagct gtacgaggtt gagcgtgacg gtaacaccat tgagtacgat ggtatgacga
     2821 tggagcgtgt ggatcgcccg acggcagagt gtgccgccgc gctggataaa gcgccgttac
     2881 cgacgccact gccgtaatga aatctccccg gcttgccgcc ggggatgaat ttaaatcacc
     2941 tcttcgataa cccacacgct gacgctgccg ccgttgcaaa agaaggtcgc ttcgccgttt
     3001 tcgtcggtca ctacgctctc ttgccgattc cccagaaaat cacgccaggt tttattgccg
     3061 taattctctc ccagatgaat ggttttttcg ccatcatccc cgttcgacat gaccaccacg
     3121 cagccgggat attcgtcagt gccactgcgg ctaaaggcaa tgcagttcgg atggtcgaaa
     3181 aataacgtct gtacaccgtg ggcgaaacgc tgacgggcga gaattaactc atcaagctgt
     3241 tcgattattg gcatatctat cggataggtt tgcccgtcac caccgacatc ttcgtaatgc
     3301 gcaccgtaga ggtccggata gaacaccgaa ggaacgccat tttcccgcaa caaaattaga
     3361 gcatacgcca gcggtttaaa ccacggttcg accggcgctt cgagggcttg caacggttgg
     3421 gtgtcgtgat tggctacgag ggttacggcg tggaaaggat cggcttccac taatgtaccc
     3481 gtgaaaatct gcgtcatgtc gtagtcgcgt cccatgcgcg atgcttcatg gaatttcatc
     3541 tgcagcggcg catcgaacag catggttttg ccttccacct gatcaatata cgtttgcagt
     3601 ttatcgactt catgcgacca gtactctgcc acaataaaca gcggctttgg cgcaacttcc
     3661 tgtacgtgtt cgatccactc tttataaaac cacgccggaa tatgtttaac cgcatcaaga
     3721 cgaaaaccgt cgcattgtgt ttgttccatc acccagcgcg cccagtattt aatctcttcc
     3781 gtcacggcat gattgcggaa atcgatattc tcacccatca gataatcgaa attaccgagt
     3841 tcatcatcaa cctgatcgtt ccagccttcg ccggtgtagt cgttaacaat tttaaatatg
     3901 ccatcttcat caggattttc gatatggtcg ataccgctaa aacatttgaa gtcccagata
     3961 aactgcgagt attgcccggc acgggcgggg aaggtgtaac gcgtccagcc ttcgcactca
     4021 atgatttctt catcaatttg cgtacggtca tcagcattta cacgctgcac gcgaatagct
     4081 tctttttcat ccgcccccat tttgtggttg actaccacgt ccagtagcac cgcaatgtca
     4141 ttacgtttca gagcgtcaat ggcggccagc agttgtgctt tatcgccata tttagtaggg
     4201 atgctgcctt tctgatcaaa ctcacctaaa tcaaataaat cataggagtc gtagccgacc
     4261 gaatacccgc ccgatgcgcc tttataggcg ggcggcaacc agaccatatt gataccaata
     4321 tcattaaaac cgtcggcgcg ctcggccagt tcaggccaga gcttaccgcc ttccgggtaa
     4381 taccagtgaa aacattgtaa cagcgtggga ttacgcattc ttcgactcca ttcaagggga
     4441 acattagaag cgtagccgta atcggattat tcgcgagcca tcggctcgtt aagattcaaa
     4501 agaggttatc ctgcggagcc agcacaaagc cgccctgatc tccataggca cttaacactg
     4561 atttttgcac cgatgactga ccgaccagtt tcgccagttc atccatacga atctgtaata
     4621 actgctttac cttgctttcg ttgtcgagaa tcaggcgcag catcgggcgg agctgctcct
     4681 gcatcgtggt ggacggggca acctcttcag tcagatgtgc aatctcctgc accgcattca
     4741 catacgccat ttcgctggcg atgagttcgt cccattgttc ttccgttgcc aggcgtaaca
     4801 tgagctggct tttatcgacg agttgttgcc aggcgaaata caaatgcggt gcatggttca
     4861 tcagactggg tcctgaatca aagaagggga gagtaaagac tctttccagg catcggcaat
     4921 attgcgcatt aatgcttcca cttcttcgac tgcggagaca tcgttgcgta aattggcttg
     4981 cagcaagcgc ctgaccatat agctataaag agcaatcaag ttttgggtta gttcgtcttt
     5041 gctctcttca tcaagactca cccgcagccc gttctcaatg atgttgatcg cttttgacaa
     5101 agagacgcct ttgccttgct gattgttgtc ctgcataaac aggcgcgctc taaccagtgc
     5161 gctcagcact ccatcaaata gcatggtgac cagctgttgc tggctggcgc tcattacggc
     5221 gctttcgacg ccaatttgtg cataggcttt tgagccgctc gcggtataca tgctgacctc
     5281 cgtgatcagg aattactcat tgcagtaaat tgttggctga gataggtact ggtgttattc
     5341 agtttgctca tcatggtatc taattgcgta aactgtgcag tgtaacgcgc cacggtgtca
     5401 tcaatgctgg cactgacgga tagatactgt ttggtcagtt ttttcagtgt ggcgttaata
     5461 ctgtcctgtg cgctatcaat aatgccatca tcggcaaggt aaccttttac ctcggtggca
     5521 attttggtgg taattcctgt ttctttacca tcgcctacca gcaattcacg tacggatgct
     5581 gtattttcat tcaggacttt tttgagttta tcatcatcaa ttttcagctt acccgtcgtg
     5641 ccgtcctgct taatcccgat ctcgtttaat gttttgaagg cactgtcgct ggcaccgttt
     5701 gcaaattgtg cgcggatgcc gctttgaatt gtacgtacaa cgctgtctcc gagaagtgca
     5761 ccattatttt tatcttgtgc ttcagcacct gggtct
//
LOCUS       DAJTVE010000198         5640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000198 DAJTVE010000000
VERSION     DAJTVE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO439_003915"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO439_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6318156.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO439_003916"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO439_003916"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6318157.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO439_003917"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO439_003917"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6318158.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO439_003918"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO439_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6318159.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAJTVE010000199         5582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000199 DAJTVE010000000
VERSION     DAJTVE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            128..295
                     /locus_tag="OO439_003919"
     CDS             128..295
                     /locus_tag="OO439_003919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318160.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            403..636
                     /locus_tag="OO439_003920"
     CDS             403..636
                     /locus_tag="OO439_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318161.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            928..1083
                     /gene="hokD"
                     /locus_tag="OO439_003921"
     CDS             928..1083
                     /gene="hokD"
                     /locus_tag="OO439_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6318162.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1387..1659
                     /locus_tag="OO439_003922"
     CDS             1387..1659
                     /locus_tag="OO439_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318163.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1733..2719
                     /locus_tag="OO439_003923"
     CDS             1733..2719
                     /locus_tag="OO439_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6318164.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGAQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2720..3100
                     /locus_tag="OO439_003924"
     CDS             2720..3100
                     /locus_tag="OO439_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6318165.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3097..3918
                     /locus_tag="OO439_003925"
     CDS             3097..3918
                     /locus_tag="OO439_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6318166.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            4143..4383
                     /locus_tag="OO439_003926"
                     /pseudo
     CDS             4143..4383
                     /locus_tag="OO439_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4491..5549
                     /locus_tag="OO439_003927"
     CDS             4491..5549
                     /locus_tag="OO439_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6318167.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
BASE COUNT         1467 a         1233 c         1585 g         1297 t
ORIGIN      
        1 atggaggctc ttgataatcg ttcgtcgatg gattggtgtg ttgctgttca tgatatgaat
       61 gcagggtttg atgaaaagat tttgttcccc tcatcagttg aaagcactgc gggttaagga
      121 gtaacacatg accactatta ccaaagaacg tattgaattg ttcattaaaa acccgcttga
      181 aaacgggctt acccgtggtg aacaaatgga actggcacgg attgcgctgg catcgctgga
      241 agcagagccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg catgacgact
      301 tttaccagag agcagttaat agctcacgca gaggagacta ttgaagcaca gagactgtgc
      361 ataccgggca caatcgacca tgacatcatc cgcacatata agatggatat tgctgttctg
      421 gaaatcgcac tggtatcgct ggcagcagag ccagccggta aattgcatga atacaaacca
      481 gtgggatatc agcgtctggt cgatgagtta accatgctgg taaagcagtt aacctggcaa
      541 ctgaggaaag cgaagccaga ctgcaaatta ccggataagg cgatgagtta tctggagcgg
      601 aacggactga taagcgtgga ggatatttta cgatgacctg gcctgaagca ttaacaacgg
      661 taggaattgc gatggcggtg gcgctggtgg tgtattcgat ttgccgctgg ggataaaaac
      721 ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg tgcttgaggc tatctgtctc
      781 aggcatgaac accaaaaggc agatagagaa aagccccagt taacattacg cgtccggcaa
      841 gacgcttaac attaatctga ggctcaatct atgaacggca aatctaggtt agcctcttac
      901 gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg
      961 atcgtcatct gtttaaccgt catagtgacg gcactggtaa cgaggaaaga cctctgcgag
     1021 gtacgaattc gaaccggcca gacggaggtc gctgtcttca cagcttacga acctgaggag
     1081 taagagacca ggcgggggag aaatccctcg ccacctctga tgggtcaggc atcctcaatg
     1141 cacccacact taacccgctt cggcgggttt ttgtttttat tttcaacgcg tttgaagttt
     1201 tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg cgcagggaga agagggatgg
     1261 accccgaaca ggggagtgct atttatctgg aaggattctg ttgatgaaaa tcgaagaatt
     1321 acgtgaaatt tttagtgaag atggcctcta tactgtgcgc gttgagaatg gcgctattgt
     1381 cagccactgc cttccagact acacaattct gataattcag ccgtctttgc cagcaggcac
     1441 gggcggcgct ctcacgcatt taaatctgat tggtaccagc atcccccatg cactgaagaa
     1501 caggccgaat ggctcattca gtgttaccgc aggcgcggat gcgaggttaa aaaagccctt
     1561 agcctcgact accgtcactg gataatctcc gtcaggctcc cttactccga acggccaacg
     1621 cgtccgtccc gcacattcca gcaacggatc tggaggtaat gtgcgggtat tacttcgacc
     1681 tgttctggta ccggaactcg gtctggttat cgttaagcca ggccgtgaat caatgtcagc
     1741 attccataac ggcagaatac tggtggagcc ggaaccaaaa agcatgcgta atctgccgtc
     1801 cggggtcgtt cctgccgctc gccagccgct ggtggaagac aaaacattgc tgccgttttt
     1861 cagtaacgca cgggtgattc gtgctgctgg tggtgctggt gcattgtctg actggctgtt
     1921 gcgccatatt aaatcctgcc agtggccaca cggcgattat catcacagcg aaaccgtcat
     1981 tcaccgttat ggtaccggcg caatggtgtt gtgctggcac tgcgacaacc agctgcgtga
     2041 ccagacatcc gaatcactcg agcaacttgc tcatcaaaac ctgtcagcat ggatgattga
     2101 cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa ttatctctgg ctgaattatc
     2161 ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg gaagcggtat tacgtcgttc
     2221 gctggggttg cgtgcggaaa aaattcgctc tgtgtacagt gaaagcgaca tcataccggg
     2281 agcgcagaca gccaccagca tactgaagca gcgcacaaaa aatattgcgt tactgcctca
     2341 cgtccaccag caacagaacc caccacagga aaagacggtg gtcagcattg ccgttgatcc
     2401 ggagtcaccg gctcagtatc tccagcgcca gaaatcacaa cgggaagaga tgcctgtata
     2461 cacgcgctgg gtaaaaacgc agaaatgcat gacgtgtggc aatcaggcag atgatccgca
     2521 tcacatcatt ggtcatggac tgggagggat gggaacaaag gctgatgatt tgtttgttat
     2581 tccgttatgc cgtaaatgcc gtaacgaact acatgccgga gtaaaagatt ttgaagagaa
     2641 acacggcagt cagctgttgt tgctgattcg ttttttaatg cacgcgagaa attcgggtgt
     2701 tctgaagtgg aaagcataaa tgaccgagcg catagaattt gttttacctt acccgccgac
     2761 ggtgaacact tactggcgtc gtcgtggcag cacatatttt gtatcaaaag ccggggagcg
     2821 ttatcgccgg gcagtggcgc ttattgttcg ccagcagcgg ctgaaattaa gcctgtccgg
     2881 aaggttggca ataaaaatta ttgcagaacc accggataag cgccgccgtg acctggacaa
     2941 tattctgaaa gcgccgctgg atgcgctgac gcatgcgggg ttgctaatgg acgatgagca
     3001 gtttgatgaa atcaatatcg ttcgtgctca gccagtatct ggtggacgtc tgggggtgaa
     3061 gatttatccc ataatgcttg aagggcaggt caaaaaatga aactggaaga tttaccgaaa
     3121 tactactccc caaaatcccc cggcctgact gatgcatcgg cctcaacgtc gaaagatgcg
     3181 ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg
     3241 gtttctgcgt tccttgggaa aatgggcatt agtatgaatg acagagagcg ggcaacagaa
     3301 ttgctgacag aatatgcact cagtcggtgt gatcgcgtgg cggcgttaag aaaactcccg
     3361 gcagaaataa aaccggcagt gatgcgtatt atggcttcgt atgcgtttga agattatgcc
     3421 cgtagcgcgg cgagcaaaaa acagtgcccc tgctgtcacg gaaaaaaatt tattgaaagc
     3481 gaggttttta caaacaagat ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt
     3541 acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa
     3601 gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt
     3661 gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga
     3721 gactgccagc gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat
     3781 gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat gggaaaaaac agttaagaaa
     3841 ttttatgatg cgctggtgac ccggtttgat attgaagaag catgggctga gcggcagtta
     3901 aaaaaggtaa ctaggtaaca aggttgattt ttccggaatc tgtggtaaat tcgtcataac
     3961 gatgggcgtt ttatgcctga cgttagaaga gtttctacaa cccgccgccg agcgggtttt
     4021 ttatgactga aatcgcgtca gtacagtaaa cgcgctggtg gcggtgaata cctgtctttc
     4081 agcttgctgg ctttttcgac aagagttatt ggtgtgtcac gttaaccgga aaaggaaaag
     4141 acatgctaaa acagcaggat atgacagaaa ccgccagagt ggtgtttaat gaattaagcg
     4201 ttaccgaacc ggcgacagtc ggggagatag cgcagaatac ttacctttca cgcgaacgct
     4261 gccagttaat actgacccag ctggttatgg cgggtctggc agactatcag ttcggttgtt
     4321 acagacgcct tccgcagtga aggctttttt atttgtggta aatgggcggc tggtgggtgt
     4381 taggggcacc caccagccat ctgctcatgc gttgggttca caagcaaacc tcaggcccac
     4441 tgctttgcgc aaaagcagaa tgagcctatc agagacaggc ttaatgatcc atgcttaata
     4501 ctgtaaaaat atccagttgt gagttaatca acgccgactg cctggaattt atccggtcgt
     4561 tacccgaaaa ttctgttgac ctgatagtca cggacccgcc gtactttaaa gtgaagcctg
     4621 agggctggga taaccagtgg aagggcgacg atgattacct gaagtggctg gaccagtgtc
     4681 tggcgcagtt ctggcgggtg ctgaaacctg ccggaagtct ttacctgttc tgtggtcatc
     4741 gcctggcatc tgatatcgaa atcatgatgc gtgaacgctt cagtgtgctg aaccatatta
     4801 tctgggcgaa gccgtccgga cgctggaacg gatgcaacaa ggaaagcctg cgggcgtatt
     4861 tccccgccac agagcgcatt ctgttcgcgg aacattatca ggggccgtat cgtccgaaag
     4921 atgccgggta tgcggcgaag ggcagtgcac tgaaacagca tgtgatggcc ccgctgattt
     4981 cttactttcg tgatgcgcga gctgccctgg ggataacggc aaaacagatt gcagatgcca
     5041 caggaaagaa aaacatggtg tcgcactggt tcagtgccgg tcagtggcag ttgccgaatg
     5101 aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag aagcatcggc
     5161 gtggtgaact ggaaaagctc caccaccagc tggtggatac gtatacctca ctgaaccggc
     5221 agtatgcgga gctgctgagt gaatataaac atctgcggcg gtattttggc gtgacggtgc
     5281 aggtgccgta taccgatgtg tggacgcata aaccggtgca gttctatccc gggaaacatc
     5341 cgtgcgaaaa accggcagaa atgctgcagc agataatcag cgctagcagt cgtccgggtg
     5401 acctgattgc agattttttc atggggttgg gttcgacagt gaaagcggca ctggcgctcg
     5461 ggcgtcgtgc aattggcgtt gagctggaga ctgaacgttt tgagcagacg gttcgggaag
     5521 tacaggattt agtcagccag aacggatgat attgaagaat taattacgcg tcgttattat
     5581 gc
//
LOCUS       DAJTVE010000200         5479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000200 DAJTVE010000000
VERSION     DAJTVE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..291
                     /gene="soxR"
                     /locus_tag="OO439_003928"
     CDS             <1..291
                     /gene="soxR"
                     /locus_tag="OO439_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6318168.1"
                     /translation="IIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            837..2186
                     /gene="ghxP"
                     /locus_tag="OO439_003929"
                     /gene_synonym="yjcD"
     CDS             837..2186
                     /gene="ghxP"
                     /locus_tag="OO439_003929"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6318169.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2338..3987
                     /locus_tag="OO439_003930"
     CDS             2338..3987
                     /locus_tag="OO439_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6318170.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4141..5432)
                     /locus_tag="OO439_003931"
                     /pseudo
     CDS             complement(4141..5432)
                     /locus_tag="OO439_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1223 a         1241 c         1413 g         1602 t
ORIGIN      
        1 attatcaaaa ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc
       61 gtgttgcccg aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg
      121 cgagaagagt tggatcggcg cattcatacc ttagtggcgc tgcgtgacga actggacgga
      181 tgtattggtt gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc
      241 ttaggagaag aaggtaccgg cgcacgcttg ctggaagatg aacaaaacta aagcgccaca
      301 agggcgcttt agtttgtttt ccggtctttg tctttctctc tatcccgctg gtacacagga
      361 gggtttcccc cgacgtcaac acaccccatt cgagcacgtg gtggaggttc cggttggtgt
      421 tgatgcttta attgtatgtc accggcattt cttcgccagt gtaaaagtat actttttatc
      481 cgcaatattt ttgtcatctc agacgatttt ttatcgcaat cctgaacggt atacggctcg
      541 ataacgctgc aatcttgcgc accgacgata acgtttgcgc atcaattgcc tggtttttca
      601 tcgtcaagac aataaaagag aaaaaagcag caaacttcgg ttgaaaaagc cgctatgatc
      661 gccggataat cgtttgcttt ttttaccacc cgttttgtat gcgcggagct aaacgtttgc
      721 ttttttgcga cgcagtaaat tgtcgcaaac ctggagcagg aagataacgt ttctctggca
      781 ggggattgtc cgccacgcat cttgacgaaa attaaactct caggggatgt tttcttatgt
      841 ctacgccatc agcgcgtacc ggcggttcac tcgacgcctg gtttaaaatt tcacaacgtg
      901 gaagcactgt ccgtcaggaa gtggttgccg ggttaacaac gtttctggcg atggtctact
      961 cggtcatcgt cgttccaggt atgttgggta aagcaggctt cccgcctgcg gcagttttcg
     1021 ttgcaacctg tctggttgcc ggactcggtt ctatcgtgat gggtctgtgg gctaatctgc
     1081 cgttggcgat tggttgcgcc atctccctga cggcgtttac cgcattcagc ctggtgctgg
     1141 ggcaacatat tagcgtacct gtcgcgctgg gtgccgtgtt cctgatgggt gtgctgttta
     1201 cggtaatttc tgccacgggt atccgtagct ggattttgcg caacttgccg cacggtgtgg
     1261 cgcacggcac ggggatcggt atcggtctgt tcctgctgct cattgccgct aatggtgtcg
     1321 gtctggtgat taaaaacccg cttgatggtc tgcccgttgc gctgggtgat ttcgcgacct
     1381 tcccggtgat tatgtcactg gtaggtctgg cggtgatcat cggcctggaa aaactgaaag
     1441 tccctggtgg cattctgctg accattatcg gtatctcaat tgtcggtttg atcttcgatc
     1501 ctaacgtcca tttctccggc gttttcgcca tgccttcatt gagcgatgaa aacggcaatt
     1561 cactgattgg cagcctggac attatgggcg cgctgaatcc tgtagtcctg ccaagcgttc
     1621 tggcgctggt gatgacggca gtatttgatg ccaccggaac tatccgtgcc gtcgccggcc
     1681 aggcgaacct gctggataaa gatgggcaga tcatcgacgg tgggaaagca ctgaccactg
     1741 actccatgag cagcgttttc tctggcctgg tgggtgctgc tccggcagcg gtatacatcg
     1801 agtctgcggc gggtacggcg gcgggcggta aaaccggttt gacggctatc accgttggcg
     1861 tgctgttcct gctgattctg ttcctctctc cgctctctta cctcgttccg gggtatgcaa
     1921 cggctccggc gctgatgtac gttggcctgc tgatgctgag caacgtggcg aaaatcgact
     1981 ttgctgattt tgttgatgcg atggcgggtc tggttacggc ggtattcatc gtgctgacct
     2041 gtaacatcgt aacaggcatc atgatcggct tcgcgactct ggtgattggt cgtctggttt
     2101 ccggcgaatg gcgcaagttg aacatcggta cggtcgttat cgccgtggcg ctggtgacct
     2161 tctatgcggg tggctgggct atctaatctt tcatcggatt ctgaaaacgg gtggcaatgg
     2221 ctgcccgttt ttattttctc cgcacattgt gtgagctttt gcgatattct gaaaaaatga
     2281 gaattcaggc ataacgcctt tctcagatca taaaaaaaca tcgcaaacag ggaacgcatg
     2341 gaaatcttct tcaccatact gataatgacc ctcgtggtct cactgtccgg ggtggtcact
     2401 cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg tgcgctactg
     2461 gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt agtcttgttt
     2521 atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt tctcgaacat
     2581 ggtagagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt cggcattggc
     2641 ttccttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc gctggcggcg
     2701 gtactttctc cgaccgatgc tgtggcgctc tccgggattg ttggcgaagg gcgcatcccg
     2761 aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcctc cggcctggtg
     2821 tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcaccgt cggcggtgcg
     2881 acggtcgaat ttatgaaagt agccattggt ggtattcttg ccggttttgt ggtgagctgg
     2941 ctgtatggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc cgcgacgcag
     3001 atcgttctgc tgttcttgct gccattcgct tcttatctga ttgccgaaca tattggcgtt
     3061 tccggcatcc tcgctgcggt tgccgccggg atgaccatca cccgctccgg tgtgatgcgc
     3121 cgtgcgccgc tggcaatgcg cctgcgcgca aacagcacct gggcgatgct ggaatttgtc
     3181 tttaacggca tggtgttcct gctgttaggt ctgcagctgc cgggtattct ggagacgtcg
     3241 ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac cgatattatt
     3301 ctgatatatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat gaaaaagttc
     3361 agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac acgagaaatc
     3421 ctgatcgcgt ctttcgccgg ggtgcgtggg gcgatcactc tggccggtgt gctctctatc
     3481 ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt gttcctggcg
     3541 gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat tctgctacaa
     3601 cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag aacgtattgc gcgagcggca
     3661 acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc ggcggatacc
     3721 gaagagaata tcgataacca gctgcttacg gaggtcagtt ctcgcgtcat tggtaacctg
     3781 cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga gaaccttgag
     3841 cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca cctgcgcgcc
     3901 acgcgggaga tcagcaacga aacgctgcaa aaattactgc acgatctcga tttgcttgaa
     3961 gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg taaccaggcc cgaggtaatc
     4021 tgaaaaaggt gcaatctttt gcacctttaa caggttagct cgggctaaca ttcatgattc
     4081 taaaacaaat aagttatctc tcttgtaaag tcaattcatt ggctgttctt cgacttaacc
     4141 tcaatctgcg tgttgcataa tatctgcagt aatcttcata gcgactgcat taatattatc
     4201 ctcagtttgt gacatgccag tatgagaggt acaatcttct tcaaagaatt tctgtagtgt
     4261 taccagggta tcactatcaa tggggatatt ttcctcccca cggagtatgg aaatgcccat
     4321 tccctggacg ctagcctcat caaaattaac ccgatagaga cgggtattaa acatacaggc
     4381 ttttgttaaa atagaacagt tgaaatggca gttgcttaaa ttggcattat taaaattaac
     4441 attagccata ttagagtcgg tacatataac catttcgagg aatgtatttg ttagccacgt
     4501 acaaaagagt ttgctatcaa ttaagtttat attctggagg attgctttgt tcagattgac
     4561 gcgagacaga tcagcatgtt caaagttaat taaatcgagt ttagagaaac taaggtcggc
     4621 tttaaataaa ttaacatatg gcgctattac tttataaaga tttgcctcag ccatgtacgc
     4681 catataaaaa tttgaatagt caagaaaggt atttttaata ttggctctat tcattgctgc
     4741 attgtagaat tgaacatgca taacggagca tgcatttaga gttccgtcgg ccatttttat
     4801 ctgagcgaaa tttgagtcat cgagaataca attatcaaaa agtatttttc cattatggcg
     4861 taataaaaat tcgttttata catattacag tcctggaaaa tagcgttttg tagtctgcaa
     4921 cctgcaaaat tcgaattgga taaatcttca tctttaatca cgataccttt caggctaagg
     4981 tgtgaaaaat cgtaaccacc atttactgtc tgtggaattt cgatagtatt attatggacg
     5041 gcgacattat ttataaaata taccacctta atggatactt ccttttcttt actaaggtaa
     5101 actcttctcg taattctatc gtcacattta ttatttgcat ataaagctat atctataaaa
     5161 tgtttatgta ttttcctgtc cgtttccttc gtgatttttc ctgatggtaa actatcataa
     5221 attcttttca ttttctgaat caaacctatc actttttcat gatcatccat cttgaaaaga
     5281 gagagggctt tattgtcaat taacgtgttt ttatgatgtt tgctgtaatt atatgatttt
     5341 gttttctttg tatgtgatga acttgtgaaa gagtggttat cgttaatctg gcaaagagag
     5401 aaaaacatat tatttaaacc attatagcgc ataaaatatc cttataatta acaagaaaga
     5461 aaaggcatat tctctgcat
//
LOCUS       DAJTVE010000201         5478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000201 DAJTVE010000000
VERSION     DAJTVE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..479)
                     /locus_tag="OO439_003932"
     CDS             complement(<1..479)
                     /locus_tag="OO439_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR6318171.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     "
     gene            complement(725..1177)
                     /locus_tag="OO439_003933"
     CDS             complement(725..1177)
                     /locus_tag="OO439_003933"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6318172.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1174..2229)
                     /gene="dinB"
                     /locus_tag="OO439_003934"
     CDS             complement(1174..2229)
                     /gene="dinB"
                     /locus_tag="OO439_003934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6318173.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2300..3085)
                     /gene="lafU"
                     /locus_tag="OO439_003935"
     CDS             complement(2300..3085)
                     /gene="lafU"
                     /locus_tag="OO439_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6318174.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3057..4769
                     /locus_tag="OO439_003936"
                     /pseudo
     CDS             3057..4769
                     /locus_tag="OO439_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4892..5389)
                     /gene="rayT"
                     /locus_tag="OO439_003937"
     CDS             complement(4892..5389)
                     /gene="rayT"
                     /locus_tag="OO439_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6318175.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
BASE COUNT         1304 a         1433 c         1432 g         1309 t
ORIGIN      
        1 gcatccagac ctgccagtgc aaacagttca gcgtcgatga tttgatcaac ttgttgcagt
       61 tcagcgaaat gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca
      121 aatgctgacg gcaggttttc ctccagccag ctttcttcag caacgtcatc caaagctgat
      181 aatcgctttt caaacttatc ggcgttgtat ttgcgagcga gaatatctgt ttgccatagc
      241 gccatgccgc agccgatatc attgccgacc agtgctgggt aaaaacggcc aacagagaag
      301 aacgctgcgc caatcgggta gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc
      361 tgcatgttgg gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac
      421 aggtcatcag atgcgatggt aaataccgca tcggataagg gacgaatata tttgcccatg
      481 taccattccg taaatagact cgtcatactt caagttgcat gtgctgcgac tcgaattatt
      541 gagagtaaca attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
      601 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
      661 gcagtttgtg gaataagaaa acgcgcacat actaggcggg cgttattttc attgcaagct
      721 ggatttaatg ttgcggttta tattgcatat aaaaattagt aaaccacgct ccccggcatt
      781 caacgtgctg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta
      841 tgctggcatc taccgtaagt tcggattcag acttaataaa tggttttaac aaggcgctgg
      901 caaccccgcg gcgggtgtat tcagggtcaa caaataacat atcgatataa tgttcaatgc
      961 gggaaataaa accaaccggt tgtgcattaa tgaccgcaac ccacacttgt gatttcgcga
     1021 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggagaat
     1081 aatgctgact ggcggtcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat
     1141 cgccaggctg atagtttctt atttgtatgt tattcataat cccagcacca gttgtctttc
     1201 catttgcgga tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg
     1261 gcgttcatcc caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca
     1321 gacgtgctcc tgagtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgggc
     1381 aatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc
     1441 gataatcgct tcacattcag accagtggtg gatatcttcc gccatcgtgc gttccacgcc
     1501 gacggatttt cgcaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc
     1561 ccacaaaatg cggccaaatt tgccaaagcg tttaagcagc atcaccagat cacacttttg
     1621 tacatcaccg caggttcgta gccccatcgc ttccagtttt gccgccgaga ctttgccgac
     1681 gccggggatt tttgccagtg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat
     1741 cacaaactgg ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttacggg
     1801 tgccacgccc gcagacgccg tcagttgcag ctcgttgaaa atcgtctggc ggatttcctg
     1861 ggcgatgagg gtcgcagaac cgtggcaatg gacgctatcg gtgacgtcga gataagcctc
     1921 atccagtgac aacggttcaa tacgcgaggt gtagcgcgag aagatttcgc ggatatgatt
     1981 tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt
     2041 gagcgccatc cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc
     2101 ggtactgatc accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag
     2161 ggcgggattg tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat
     2221 tttacgcatt gctcacctct caacactggt aaagtataca gtgatttcag agtttgagaa
     2281 atgcgtaaag attcagcatt taacgcgttc gctgcgaaag cacctgctgc ttatccagct
     2341 tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat
     2401 cggacgcact tttggtcagc accataatct caatgcgccg gttgcccgcg ctttgcggat
     2461 ttttggaatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg
     2521 gcattccggc ctcttccagc acccgacgag ccgaaagcgc gcgatcgccc gaaaggttcc
     2581 agttgttgta gatattgttt ttgtaagcca tcgcatcggt atgcccggta ataataattt
     2641 tgttatcgag cgagtcgaaa actggcgcga gctccaccag cagcgtttta aagaacggca
     2701 taatctgcgc gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagcacgc
     2761 gtaatccctg gggaacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa
     2821 tggtggtgat actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg
     2881 ccgttttctc attgacgtcg cgcgcttttt tctccgtttc ttccggcacc gccacggtgg
     2941 caggttttgg cggatgagac gggctgattt tatttaacgg cgacagcccg ccaccgttaa
     3001 aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt
     3061 tgacaatcaa ctttattgtc gtcaccaaag gggccgagcg tatctccgag gtttctgccc
     3121 gctttaccct ggacgcgatg cccggtaagc agatggcgat tgacgccgat cttaatgccg
     3181 ggttgatcaa tcaggcgcag gcacaaaccc gacgtaaaga tgttgccagc gaggccgatt
     3241 tctacggcgc gatggacggg gcatcgaaat ttgtgcgcgg ggatgctatc gccgggatga
     3301 tgattctggc gatcaacctg atcggcggcg tctgtatcgg gatcttcaaa tacaacctga
     3361 gcgccgacgc cgccttccag cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc
     3421 aaatcccttc cctgctactc tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca
     3481 acggcgatat cgcccatgac gtgcgtaacc agttgctggc aagcccatcg gtgctctaca
     3541 ccgctaccgg aattatgttc gtactggcgg tagtgccggg aatgccacat ctgccgtttt
     3601 tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg
     3661 cggaagcgga agagaaaagt ctcgaaacgc tgacccgcac catcaccgaa accagcgagc
     3721 aacaggtcag ttgggaaacc attccgctga tcgagcccat cagcttaagc ctcggctaca
     3781 agctggtggc gctggtggac aaagcccagg gcaacccgct cacccagcgt attcgcggcg
     3841 tgcgacaggt gatatccgac ggcaacggcg tgctgctgcc ggagatccgc attcgggaaa
     3901 acttccgcct taagcccagc cagtacgcta ttttcatcaa cggcattaag gctgatgaag
     3961 cggatattcc ggcggataaa ctgatggcgc tgccctccag cgaaacctac ggcgagatag
     4021 acggcgtgct ggggaacgac ccggcgtacg ggatgccggt gacctggatc cagcctgcgc
     4081 agaaagcgaa ggcgctgaat atggggtatc aggtgatcga cagcgccagc gtgattgcta
     4141 cccatgtaaa taagattgtg cgcagctata ttcctgattt atttaactat gacgatatta
     4201 cgcagttgca taaccgtttg tcgtcgatgg caccgcgcct ggcggaggat ttaagcgcgg
     4261 tgctcaatta cagccagttg ctgaaagtgt accgggcgct gctgaccgaa ggcgtttccc
     4321 tgcgcgatat cgtcaccatc gccaccgtgc tggtcgccag tagcgcggtg accaaagatc
     4381 atattctgct ggcagccgat gtgcgcctgg cgctgcggcg cagcattacc catccgttcg
     4441 ttcgcaagca ggagctgacg gtgtataccc tgaataatga gctggaaaat ctgctgacta
     4501 acctagtgaa tcaggcgcaa cagggcggta aagtgatgct cgacagcgtg ccagtggacc
     4561 cgaatatgct caaccagttc cagagcacga tgccacaggt gaaagagcag atgaaagcgg
     4621 cggggaaaga cccggtgctg ctggtgccac cgcagctgcg ccctttgctg gcgcgttatg
     4681 caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg gacgagctgg
     4741 agttgaagat tatgggggcg ttgatgtaag tatccggcat tggtgcacaa ctgccggatg
     4801 cggcgtaaac gccttatccg gcctaccgtt caggcacaag tttgtaggca tgataagacg
     4861 cgccagcgtc gcatcaggca tctgcgcata attataaaat gatacgctcg ccagcattta
     4921 tatccgttac gtcccccgcc caatcgatgg gatataaccc tcgcgcgaca tcgcgatgga
     4981 acgttgagaa tggccaatca ctcacttgct ttacccaacc atgctttact ggatttatat
     5041 aaatataatc aacatgatgc ctataatctt tggtattgcg gatggcgtgc tcccaaaaac
     5101 gtggttgcca gatatttttc aatccacaag catgggtaaa ttgcttttta atttcccgcc
     5161 agcgagagga aaaatcatca tcgccttcag gtaatgtcca tatgcaatgc atatgctctg
     5221 gcaaaacgac ccaggcgttg atttcaaaag gcctgtctcg cttaacttta ataatggcgt
     5281 tacggagcat ctggtactgg gtggtcaaga gttggcttcg acgattgcgt aaattcaccg
     5341 tgaaaaacca ggttcccccc ttgatgtaat aacgacgata ttcagacatg gcgactcctt
     5401 tttctcaacg gtaagaagaa aaggaaaggt cggcaatgag gtgatggaac agggcaagat
     5461 tacgaaagcc cgctcccc
//
LOCUS       DAJTVE010000202         5469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000202 DAJTVE010000000
VERSION     DAJTVE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            547..894
                     /locus_tag="OO439_003938"
     CDS             547..894
                     /locus_tag="OO439_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6318176.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            947..1342
                     /locus_tag="OO439_003939"
     CDS             947..1342
                     /locus_tag="OO439_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6318177.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1383..2126
                     /gene="cmoA"
                     /locus_tag="OO439_003940"
     CDS             1383..2126
                     /gene="cmoA"
                     /locus_tag="OO439_003940"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6318178.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2123..3094
                     /gene="cmoB"
                     /locus_tag="OO439_003941"
     CDS             2123..3094
                     /gene="cmoB"
                     /locus_tag="OO439_003941"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6318179.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3259..>5469)
                     /gene="torZ"
                     /locus_tag="OO439_003942"
     CDS             complement(3259..>5469)
                     /gene="torZ"
                     /locus_tag="OO439_003942"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6318180.1"
                     /translation="TAADQVHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQ
                     ALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDY
                     STGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGL
                     EYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKH
                     DKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTML
                     MAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEM
                     SAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGN
                     FTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQ
                     HIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGA
                     RAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSK
                     TLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVA
                     DREPITIHTEDAARFSIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLE
                     NGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1268 a         1392 c         1314 g         1495 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggacttcct tatgagactg gttaatagag cgctatgaag
      121 attcaggagg gtaactataa ccatcacttt ctaactttca cgtaggaaat cgttaagttt
      181 cttgatagtt acaccatgtg accaactatc tatatatcgt tatatagccc tgtatatagc
      241 actatatgta gtgtctggag agggacgact aacacgatac agtttaccct gaaaagagac
      301 ttcacagatt ctttatatat ttcaataagt tatgctaaaa agagactata ccgatactgc
      361 atagaatttg tgtaggattg catagatact tggaaaaaca ccgttaaatt gcatagtcat
      421 tcacctgata ttgtcctgtt tttacaggta gttgattcgg atgttctagg aatcatcctc
      481 atagttgatt cggttagttg ggcttgtacc aacccctaat caacctcata gtgtatttag
      541 accttgatga ttttagacag ccgccctgtt catgcagcac gtccacacag tgaagctatt
      601 cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat
      661 ccgctgattc gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc
      721 tggttacaga aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg
      781 ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg
      841 cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc
      901 cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct
      961 atatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg
     1021 aatgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg
     1081 cattcatggt aacgcggtgg aatatattcc catcgcgatt gtgctgatgc tgtttatgga
     1141 aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg
     1201 tctgatgcat tattacggtt ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag
     1261 cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga
     1321 gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg
     1381 ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct
     1441 ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt
     1501 ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg
     1561 tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc
     1621 atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa cgctgcagtc
     1681 gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg
     1741 atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac
     1801 cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg ggcggcgcgc
     1861 tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ttgttcaaca
     1921 tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca
     1981 gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata
     2041 aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg
     2101 cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa
     2161 aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga
     2221 gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc ctgaaattaa
     2281 accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc cactgagcac
     2341 cgggcaaatt aaacgcattg aaacgcttat gcggaacctg atgccatggc gcaaagggcc
     2401 gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg
     2461 tgttctgccc catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag
     2521 cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc
     2581 tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta acgatcagcg
     2641 cgcacatttg ttaccgttag gtattgaaca gcttccggca ctgaaagcct ttgataccgt
     2701 cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct ggcagttgaa
     2761 agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg atggcgatga
     2821 gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc
     2881 ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgt
     2941 ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg ttaccgaatc
     3001 tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc
     3061 taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg
     3121 cgtgaatgcc ttatccggcc tacaaaccct tgctaattca atctattgca gggactatgt
     3181 aggcctgata agcatagcgc atcaggcagt tttacgtttg cataacctca gcgcccgttt
     3241 ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg ttaacttcgg
     3301 tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc
     3361 cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc
     3421 gttttccaga tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc
     3481 agtcaccacc gcgcctgtca aaatctgacc acgtttgttc cagacacgca ccagatcgcc
     3541 attcgcaata ctaaatcgag cagcatcttc ggtgtgaata gtgatcggtt cacgatcggc
     3601 aaccgcatat tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg
     3661 atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc
     3721 aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttttggagta
     3781 aatctcaatt ttgccacttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc
     3841 accataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg
     3901 ctgccagaag gcattaaaca ttggcatagt gacgcgttgc gcacgggccc ctttctgagc
     3961 ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt
     4021 tccgccaggt ttgagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc
     4081 aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt
     4141 catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcgtgttt
     4201 agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt
     4261 aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat
     4321 tttgatattc ggatacgttt gctctttacc gttatgctga tatttgccgc caggattttc
     4381 cagcgcatcg acgatacggg caacaggaat cgccctcatt ccgccatcat cagcggcctc
     4441 gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg
     4501 gttgccacca ttggagtaat gataagagaa accaaaaccg ccgcccggtg taccaatctg
     4561 ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat attgctggcg
     4621 ctgaattccc cagcctgcca tcagcattgt gcgattcgcc gccatcagtt cagcgagttt
     4681 gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc tcttcggcgt
     4741 attatcgctc ttacctctca gatactcttc aaactgcgga tacccggtag tgtatttttc
     4801 aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag
     4861 tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa agaagtcgat
     4921 ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg
     4981 gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg tattcagcgg
     5041 gttcatcccc cacagcacca caacctggct gttttccaga atcagcggcc aggaggtctg
     5101 ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg ccgccccggt
     5161 tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa
     5221 ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg caaaaatagc
     5281 cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg
     5341 ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt gcagtggatt
     5401 atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt gtacctgatc
     5461 cgccgccgt
//
LOCUS       DAJTVE010000203         5466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000203 DAJTVE010000000
VERSION     DAJTVE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(267..638)
                     /locus_tag="OO439_003943"
     CDS             complement(267..638)
                     /locus_tag="OO439_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318181.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(699..1196)
                     /locus_tag="OO439_003944"
     CDS             complement(699..1196)
                     /locus_tag="OO439_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318182.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1272..2060)
                     /gene="aadA1"
                     /locus_tag="OO439_003945"
     CDS             complement(1272..2060)
                     /gene="aadA1"
                     /locus_tag="OO439_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6318183.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2118..2642)
                     /gene="sat2"
                     /locus_tag="OO439_003946"
     CDS             complement(2118..2642)
                     /gene="sat2"
                     /locus_tag="OO439_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6318184.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2737..3210)
                     /locus_tag="OO439_003947"
     CDS             complement(2737..3210)
                     /locus_tag="OO439_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6318185.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3542..4078
                     /locus_tag="OO439_003948"
     CDS             3542..4078
                     /locus_tag="OO439_003948"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6318186.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4193..4519
                     /locus_tag="OO439_003949"
     CDS             4193..4519
                     /locus_tag="OO439_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6318187.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4707..4946)
                     /locus_tag="OO439_003950"
     CDS             complement(4707..4946)
                     /locus_tag="OO439_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6318188.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1423 a         1303 c         1218 g         1522 t
ORIGIN      
        1 gcagggattt cttggccttg cctcatgctg atagcttgta aatatcacac gatccttagc
       61 cccacaaagt gagcatactc cgtagctttc atttatagaa atcgtcgtta gattgtcgca
      121 gcatgtgcac gttccgacta tttgctcagg aagcatcgcc ataccttggc ctaggtaaag
      181 aagatcagac tcgtagccac atcggtggca actgagcttg tacgcaatgc ccatctggtt
      241 gtctcacctg aatcgtaacg tcagagctat tcatccatcc attcggcatc aatgtatgcc
      301 actaagccga ccagctgacc gtttggactg aagtatgttg cctgcacccc acttgacatc
      361 tcatcaatac caccgcactg cgctaggcgc cggtcccact tgcccacaga agcgtcagga
      421 tcggctgagt tccccaagac ccttgtgagc catgcctcct ttaactcaac ctctcgaatg
      481 attgcgtcgg ccagctcctc cgcgttatct agttcgacat agtctggaat tcggacgtac
      541 gttctgaaca cagctggata cttacttggg cgattgtcat acatgacatc aacaatgtac
      601 ccgtttgtgt aaccgtctct tggaggttcg tatgacacta gtggttcccc tcagcttgcg
      661 actagatgtt gaggcctaac attttattag agagcaggct agttgcttag atacatgatc
      721 ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac caatgccccg
      781 cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt gtagaacatc
      841 cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac gtagtagccg
      901 gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt gcgactattg
      961 tcgtaattgg atgaactatt atcgtagttg ctctcagagt tgtcgtaatt tgatggacta
     1021 ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag aaatgtcgta gttggatggg
     1081 gagtagtcat agggaagacg agcttcatcc actaaaacaa ttggcaggtc agcaagtgcc
     1141 tgccccgatg ccatcgcaag tacgaggctt agaaccacct tcaacagatc gcgcatagtc
     1201 ttccccagct ctctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga
     1261 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtgaacaaat
     1321 tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata agcctgccta
     1381 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     1441 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     1501 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     1561 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     1621 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     1681 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     1741 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     1801 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     1861 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     1921 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     1981 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     2041 gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg
     2101 tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc
     2161 gctgtttcgt tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga
     2221 gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat
     2281 cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc
     2341 gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg
     2401 ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag
     2461 caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca
     2521 cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca
     2581 atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc
     2641 atatgacgcc taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa
     2701 ggcgtgacag gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta
     2761 actataattt atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat
     2821 ttcaggaaag taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc
     2881 tacttgatcg atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt
     2941 tattttcttt aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt
     3001 gtcagatgta aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat
     3061 tgattcaaaa gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag
     3121 gagctgttca cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt
     3181 cgatatagct accattagtg atagtttcac aattcttcct cagaggttaa cattttatta
     3241 gtaagcatgc tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta
     3301 ttaaatagac tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc
     3361 tatcctgctt gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt
     3421 tagcattaaa ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact
     3481 tttttaaatc ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt
     3541 tatgtctaac agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc
     3601 gctgaaaact gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa
     3661 acgtcatcct cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa
     3721 cagcagacat gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta
     3781 caacaggttt ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag
     3841 acggctaccc tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac
     3901 tcgcaaccaa gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt
     3961 gcgtttgcgg gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa
     4021 gggtgggaaa agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact
     4081 cattgagcaa gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct
     4141 gccttttgct ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt
     4201 ctttccctcc agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct
     4261 gcatgactcc gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag
     4321 caagcgtgtc acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg
     4381 gcgtgatatt cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat
     4441 ctatacgcat gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat
     4501 gctacttatc aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga
     4561 tactttttat ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac
     4621 cattgttcag ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta
     4681 cacttcctcc agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag
     4741 cttaggtgtt ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg
     4801 cgtttctgaa gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct
     4861 tggagtaaca agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga
     4921 tcggagttat agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt
     4981 attattgtag ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg
     5041 tatattaatt tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat
     5101 caagataact gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat
     5161 acgggatcac tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact
     5221 gatgaaagga ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct
     5281 agattagtta accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     5341 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     5401 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaagggc
     5461 tgccga
//
LOCUS       DAJTVE010000204         5463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000204 DAJTVE010000000
VERSION     DAJTVE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..116)
                     /locus_tag="OO439_003951"
     CDS             complement(<1..116)
                     /locus_tag="OO439_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6318189.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="OO439_003952"
     CDS             complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="OO439_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6318190.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="OO439_003953"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="OO439_003953"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6318191.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="OO439_003954"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="OO439_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6318192.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="OO439_003955"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="OO439_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6318193.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="OO439_003956"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="OO439_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6318194.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5351..>5463)
                     /locus_tag="OO439_003957"
     CDS             complement(5351..>5463)
                     /locus_tag="OO439_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HCR6318195.1"
                     /translation="NAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1445 a         1384 c         1340 g         1294 t
ORIGIN      
        1 ttttcgagac gttttttcat taaatccaca ctgacgaggg taaaatcctt tccgctctca
       61 cgcagggtga tatgggaagc gagagagcag gcacccggtt tgtagaacaa tttcatcggt
      121 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaagccgct
      181 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
      241 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
      301 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
      361 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
      421 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
      481 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
      541 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
      601 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
      661 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
      721 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
      781 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
      841 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
      901 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
      961 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
     1021 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
     1081 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
     1141 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
     1201 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
     1261 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
     1321 aagatgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
     1381 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
     1441 caccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
     1501 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
     1561 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
     1621 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
     1681 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
     1741 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
     1801 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
     1861 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
     1921 cagcgttaaa tttttcaaaa aacgtaatgg cagatttttt cactgcactg agaggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tag
//
LOCUS       DAJTVE010000205         5272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000205 DAJTVE010000000
VERSION     DAJTVE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..472)
                     /locus_tag="OO439_003958"
     CDS             complement(<1..472)
                     /locus_tag="OO439_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6318196.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(472..1392)
                     /locus_tag="OO439_003959"
     CDS             complement(472..1392)
                     /locus_tag="OO439_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6318197.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1530..2429
                     /gene="pgrR"
                     /locus_tag="OO439_003960"
     CDS             1530..2429
                     /gene="pgrR"
                     /locus_tag="OO439_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6318198.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2766..4379
                     /gene="mppA"
                     /locus_tag="OO439_003961"
     CDS             2766..4379
                     /gene="mppA"
                     /locus_tag="OO439_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6318199.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4430..>5272)
                     /gene="ynaI"
                     /locus_tag="OO439_003962"
     CDS             complement(4430..>5272)
                     /gene="ynaI"
                     /locus_tag="OO439_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6318200.1"
                     /translation="VIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQQIK
                     KGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS
                     NFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSS
                     ISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQF
                     ADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPP
                     EQGR"
BASE COUNT         1423 a         1204 c         1226 g         1419 t
ORIGIN      
        1 cgccaatcgg atcgtaattg ctgaaacctt cccgaaaaag acgcccgata ttaacacccg
       61 taatactcac tactgcttta attcgttttt ctgtcagcgc ggcattaatg gcatagccac
      121 cacccccaca cactcccagc acgccaatac gttcagcatt aacatatggc agagttactg
      181 cataatcgat aacacggcta atatcctcta cgcgctgtgt cggatcttct atccagcgag
      241 gctccccacc agattctccc tggaaactgg cgtcataagc gagaacgaga tagccttttt
      301 cagccaatgc cttgccatag atattgccag acgtctgctc cttacaactg ccaaaaggat
      361 ggacgctgat aatcgccgga tactgttttg actcatcaaa atttggtgga aactgaatat
      421 tggcagcaat atgccaggcc atatcactat tgcggatttg aatatgtttc attacagagt
      481 ttcctcaaag aacggagcta atactgaaat tgcttcagcc acataggctt tgccgtcata
      541 aagatccata tggtttgcgc cttcaacaat gtgatagcgt ttatcttggc ttgatgctcg
      601 atcgtacagg tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgcat
      661 cggctgagtc aggtacactt ccgccatatg gtaagcatca aaggtaataa tctggttaag
      721 gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tgtggtaata
      781 ctcccaggcc tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg
      841 cataacggca tattctccgc tgctaatatc gctggttcgg gcgtttgaac ccgcttcaac
      901 gtatggcagg gcatcaatag atttcacatt gttttcccaa ccattacgga acatcgaacc
      961 aatattgacc gcactaacgg taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt
     1021 ggctgtatat cctgcaccgg cacaaattcc catcgcacca attcgggtat tgtcgacata
     1081 agaaagcgtt gtcaggtaat caatcacggc actgatgtct tcagtacgaa tgtatgggtt
     1141 ttctaactga cgcggctcgc cgccactttc accttgataa gatgcgtcat aagcaatagt
     1201 gacaaatccc ttttccgcca gttttttggc ataggttccg gccgtttgtt ctttaacgcc
     1261 cccacctggg tgagatacca caattgcctg atactgacgg gtttcatcaa attttggagg
     1321 gaaatagatc actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt
     1381 attattcatc attccgttcc tcatggagtt gtcggttcgt tttaacggtt ggtgatatca
     1441 ctatagatat tgatcattaa gttgattaga cccaaaatta tgattagacc tatcatttaa
     1501 atgattaata gattgcatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg
     1561 cgtttgtcgt cgttgcagag gagcgtagct tcactcgtgc agcagcccgc ctgagcatgg
     1621 cgcagtcagc tttaagccag atagtgcgtc gtatagaaga acgattggga ttgcggcttc
     1681 tgacgcgaac cacgcgcagc gttgttccaa ctgaagcggg cgagcatctt ttgtctgttc
     1741 ttggccctat gttgcatgac atagattcag ccctggcatc cctgagcgag ctgcagaacc
     1801 gcccatccgg gacaatacgt attactactg tagaacatgc agccaaaacg atattgttac
     1861 cagcaatgcg cacattcctg aaatcgcatc ctgaaattga tattcagctc accattgatt
     1921 atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga
     1981 tggataaaga tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct
     2041 caccggatta tttttctcgc cgaagtgttc caacgtcagt gtcacaatta atagatcatc
     2101 aggcaattaa tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg
     2161 gtggacgtga agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga
     2221 tcattgatgc tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc
     2281 gggctattaa agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg
     2341 gttatcacct gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag
     2401 ataggctgaa gtataaaggt gctgtttagc actacttgct gatacattaa tttaattttt
     2461 ctcttaacgt attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg
     2521 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc tgaacgtagt
     2581 gttcggtgtt ctatgtgctt taccgcattt tgtcgtctat tatttacact aaatctgatt
     2641 taatatattg atacataaaa cattttatgc tccctgtgtc acttttttga tggaagttgt
     2701 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
     2761 aacttatgaa gcactctgtt tcagtcacgt gttgtgcgct gttggtcagc agcatttctc
     2821 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
     2881 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
     2941 ttcaggtcat tcgcgatctg tttgaaggac tggtgaatca gaacgagaaa ggggagattg
     3001 tccccggcgt tgcgactcag tggaaaagta atgacaaccg tatctggact tttaccctgc
     3061 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
     3121 ggcagcgtct ggtggacccg aaaacgctat cgccatttgc atggtttgcc gcgctggcgg
     3181 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gccagaccag cttggcgtca
     3241 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
     3301 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
     3361 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gatcgcgtag
     3421 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtac
     3481 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg
     3541 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     3601 atattccggg gcaggtttat acgccgccgc agctcgggac ctattattat gcgtttaaca
     3661 cgcaaaaagg gccgacggca gatcagcgcg ttcgtctggc attaagtatg acgatagatc
     3721 gccgcctgat gaccgaaaaa gtattaggga cgggcgaaaa gccagcgtgg cattttacac
     3781 cagatgttac cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     3841 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     3901 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     3961 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     4021 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg
     4081 attataatga accctccact ttcctgacat tattaacgtc aacgcattca ggaaatattt
     4141 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     4201 ttaaagcgcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     4261 ttgcaccaat ttatcaatat accaatggac gattaatcaa gccgtggctg aaaggttatc
     4321 ccattaataa cccggaagat gtggcgtaca gccggactat gtatattgtg aagcattgat
     4381 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct
     4441 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     4501 cgtgtgactg cacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccatt
     4561 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcggcaa
     4621 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     4681 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     4741 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     4801 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     4861 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     4921 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     4981 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     5041 tcatgccgaa atgttcgcca taaagcagaa caagcaccac aataatggtg attttgataa
     5101 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     5161 actgattaat cagcaaaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     5221 taaaatcgat aacgtctggg gtgataaatt tgagtttata gttttctata ac
//
LOCUS       DAJTVE010000206         5176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000206 DAJTVE010000000
VERSION     DAJTVE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..619
                     /locus_tag="OO439_003963"
     CDS             <1..619
                     /locus_tag="OO439_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6318201.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQ
                     IETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSL
                     RVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLN
                     GTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            687..4229
                     /locus_tag="OO439_003964"
                     /pseudo
     CDS             687..4229
                     /locus_tag="OO439_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1251 a         1284 c         1651 g          990 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgctgaaacc attggcaaca atcagatgat tgcggttacc aacaatcaga tagagacggt
      121 gggcgttaac cagatagaga cggtgggcag taaccagata attaaagtgg gttctgttca
      181 ggtggaaacg attggacttg ttcgtgcgct gacggtgggc gtggcgtatc agacgacggt
      241 aggtggcatt atgaacacct cggtggcact gatgcagtcc tcgcagatcg gtttgcataa
      301 atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta ccttcaccgt
      361 tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg gtgagcatct
      421 ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa tttttctcaa
      481 cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa
      541 ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc
      601 ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac cagcattaaa
      661 aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt cagggagata
      721 tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
      781 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
      841 tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
      901 tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg gtgggcgttt
      961 tcggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgtgat gacggactga
     1021 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     1081 tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg taaggcagca cagccggacg
     1141 gccatacgct ggcgcggctg tggggggcgc tgcgccggat atccggttaa gcccgcatct
     1201 ttacctggcg accaacagcg cacaggggcc gtggtggata ctgggggggg ctgagcgggt
     1261 gccgggtgct gaggacgtac tgccagcgcc gctgccgccg taccgggtgc ttaccgggat
     1321 ggcggacgct gacgtaccgg cgtgaggccg ccggtgacct ggccggggaa atcaccgacg
     1381 tgacggacgg tgccgggcgg gagttccgtc tggtgctgac cacgcaggcg cagcgtgcgg
     1441 aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc tcagcctcag
     1501 cgttccccga cacactgccc ggtaccgaat acggccccga caggggtatc cgcctttcgg
     1561 cggtgtggtt gatgcacgac ccggcatacc cggagagcct gcccgctgcg ccactggtgc
     1621 ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg
     1681 tgcgcgcttt cacgtatgac gcgcagcacc cgggccggat ggtggcgcac cgttacgcgg
     1741 gaaggccgga gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc
     1801 cggcagggtt aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc
     1861 tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa
     1921 aagaactggc ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg
     1981 cgcagacgga cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata
     2041 tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc
     2101 agctgacggc ggtggtgtac ccggacgggc tggagagccg ccgggaatat gatgaaccgg
     2161 gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg
     2221 cgcacagtga gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgt
     2281 ggagccgcta cgggcagttg ctggcgttca ctgactgctc gggctaccag acccgctatg
     2341 aatacgaccg cttcggccag atgacggcgg ttcaccgcga ggaaggtatc agcctttacc
     2401 gccactatga caaccgtggc cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc
     2461 agtatgagta caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga
     2521 gcgagacaca gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc
     2581 gcagtatgga gtacgatgct gccggacgtg tcatcagtct gactaacgag aacggcagtc
     2641 acagcgactt cagctacgat gcgctggacc ggctggtaca gcagggcggc tttgacgggc
     2701 ggacgcaacg ttatcattat gacctgaccg gaaaactcac gcagagtgag gatgaggggc
     2761 ttgtcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg
     2821 acccggcaga gcagtggcag tatgacggcc acggctggct gagagaaatc agccacctga
     2881 gcgaaggcca ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg
     2941 aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac
     3001 acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg
     3061 agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg
     3121 tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg caccctcgca ccggacctgg
     3181 acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctac
     3241 acccggacag cacgctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg
     3301 tttaccacta tgatgaatac ggcagactga cggagaagac ggaccgcatc ccgacgggtg
     3361 tgatacggac ggacgacgag cggacccacc actacgacag ccagcaccgc ctggtgttcc
     3421 acacgcggat acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctgg
     3481 gacggcgaat ggcgaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcgc
     3541 tgtcgcgtaa accggaggag acatggtacg gctgggacgg cgacaggctg acgacggtac
     3601 agaccgacac cacacgtatc cagacggtat acgagccggg aagcttcacg ccgctcatcc
     3661 gggtcgagac agagaacggc gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc
     3721 tgcagcagga agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc
     3781 tgctggacag actggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggtgt
     3841 ggcttgcgca gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca
     3901 caccagtgcg aaacgttcat ctttaccact gcgaccatcg gggccagccg ctggcgctca
     3961 tcagcgaaga cggcgatacg gtgtggagcg gggcgtatga tgagtggggc aaccagctaa
     4021 atgaggagaa cccgcatcat gtgtatcagc cataccgtct gccggggcag cagtatgatg
     4081 aggagtcggg gctgtactac aatcgtcacc ggtactatga tccgttgcag agacggtaca
     4141 tcacccagga ccccattggg ctgaaaggtg ggtggaatct gtatcagtat ccgctgaatc
     4201 cggtgagcag gatagatccg ttggggttat tctcatgtga gagttgtagt cattttatag
     4261 atgatataaa taataaactg gataaccttc cgtttaaccc gacaaaaatg tttacagcta
     4321 gtttaaatac tgctaattca gcaaggctgt atgcaagtgg catgacaaaa ttcggtataa
     4381 gtgttgctgc cgatggaacg atcattgctg ccccagtcgg tgcagcttct atggctttag
     4441 gggcgtggaa tataaaatct gcgacagctg catataacag agcaaattta caattatcag
     4501 aatcacttca ggaaaatggt agagataggt gttggagaaa tttattgggt attttacctt
     4561 atggtgagca gtttgatgac cctggtgaac catcaatatt agacgtttat ggtgaaaaga
     4621 ttgagaatat caaagattct ccaatgaagt tttttgaaga gcttgggacg ctggggttgt
     4681 aatgtgtatt tttaatgata tttttatgtt tgtgtttatt gttagtagta tacttgtaac
     4741 cgtgcatttt gtgtattctt tattttttaa aagtggaaag gtaaaagata aggtaaaaaa
     4801 atggattaaa gtaatgtgtg atttattttg ggggatagga tagtggtgtg atatggataa
     4861 atataaatta cagtggctgg catatttcga cctgacgccg gagtagataa aaaaacagac
     4921 aggattggag tatagcaagc gtctgacaat ctctcgggac agtagtatga tgaagaggcg
     4981 gggttgtact ataaccgtaa ccggtactac gatccgctaa agggcaagta tatgactcag
     5041 ggaaagatca tcagaagttc actttttgta ctaaataatt cgcattttat gtttaaaaat
     5101 tgagatattc ctcattacct gaagctgttt ttttattgct tatacataat caaatactcc
     5161 ttacataatt aaggag
//
LOCUS       DAJTVE010000207         5157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000207 DAJTVE010000000
VERSION     DAJTVE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            343..870
                     /locus_tag="OO439_003965"
     CDS             343..870
                     /locus_tag="OO439_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6318202.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGENYGFSDDIPF"
     gene            928..1161
                     /locus_tag="OO439_003966"
     CDS             928..1161
                     /locus_tag="OO439_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCR6318203.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1220..3178
                     /locus_tag="OO439_003967"
     CDS             1220..3178
                     /locus_tag="OO439_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6318204.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            3233..3667
                     /gene="psiB"
                     /locus_tag="OO439_003968"
     CDS             3233..3667
                     /gene="psiB"
                     /locus_tag="OO439_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCR6318205.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3664..4383
                     /locus_tag="OO439_003969"
     CDS             3664..4383
                     /locus_tag="OO439_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCR6318206.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            4380..4976
                     /locus_tag="OO439_003970"
     CDS             4380..4976
                     /locus_tag="OO439_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318207.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
BASE COUNT         1142 a         1365 c         1610 g         1040 t
ORIGIN      
        1 gcctgatggc gcagcagtcc tccctcctgc cgccactcct gcggcctgtt tcccgctgtg
       61 gactttccgg gggacagacg taaccccctc ccgttccatc tgtccagggt gagatgcacc
      121 ggccacaaaa tttttagcca tgaacccggc aaaaaatttt gcgtccgccc ccggacagct
      181 gccccgtgag gcggtaaacg ggcccccatc ggaaaggtcc actgcgggga gaaacccctg
      241 tgaagtggac ggcaggagga gctttcgctc ctctgaaggg gcacatgaac gtcccgtcgc
      301 cggtatccgg tttatctcac atctttcagt caggaggttc ttatgagtgc acgtggtatc
      361 aacaaggtca tcctcgtcgg gcgtctgggc aatgatccgg aagtccgtta catccccaac
      421 gggggcgcag tggcaaacct gcaggtggct acgtcagaaa cctggcgcga caaacagacg
      481 ggggaaatga aggaacagac ggaatggcac cgcgtggtgc tgttcggcaa gctcgcggaa
      541 gtggctggcg aatatctgcg caagggcgcg caggtctaca tcgaggggca actccgtacc
      601 cgtagctggg aagataacgg tatcacccgt tacgtcactg aaattcttgt taagaccacg
      661 ggcaccatgc agatgctggg gagtgcacca cagcagaacg ctcaggcgca accgaagcct
      721 cagcagaatg ggcagccaca gagtgctgac gcgacgaaaa aaggtggcgc gaaaacgaaa
      781 ggccgtggac gtaaggccgc gcagcccgag cctcagccgc aaacgccgga gggggagaat
      841 tacgggtttt cagacgacat cccgttctga tcgggctgac tgtgacaacc gccccgccct
      901 gtgcggggca tcaccggaga gatgaggatg agcgaatatt tcagaatact tcagggactg
      961 ccggacggct cctttacccg cgaacaggcg gaagccgttg ccgcacagta ccggaacgtc
     1021 tttatcgagg atgatcaggg aacgcatttt cgcctggttg tccgcaataa cagtggcctg
     1081 gtctggcgta cctggaattt tgaggacggt gccgggtact ggatgaacca tgccatccgt
     1141 gatttcggga ttcttaagta agagaggtgc cggacgcgcg aacgtccggc aggacataag
     1201 caattataag gggatgatta tgcctgtaac gaagtgtgaa ccagaaacca cccgcaaagc
     1261 aagccgtaaa tctgcaaaaa cgcaggaaac tgtcctgtct gccctgctgg cgcagacgga
     1321 ggaagtgagc gtgccgctgg cctcgctgat taaatcaccg ctgaatgtgc gcacggtgcc
     1381 gtattctgcg gagtccgtca gcgaactggc tgagtccatt aagggcgttg gcctgttgca
     1441 gaatctggtc gtgcatactc tgcctggtga ccgttacggt gtcgccgcag gtggtcgccg
     1501 actggctgca ctcaacatgc tggcagagcg tggcatcatt ccggctgact ggcctgtccg
     1561 tgtgaaggtc attccgcagg agctggcgac ggcggcatca atgactgaaa acggtcatcg
     1621 tcgggatatg caccctgctg aacagattgc cggattccgc gcaatggcgc aggaaggcaa
     1681 aacacctgca caaatcggtg atttgctggg ctattcaccc cgccacgttc agcgaatgct
     1741 gaaactggct gaccttgcgc ctgtcatcct cgatgcgctg gcagaagacc gcatcaccac
     1801 cgaacactgt caggcgctgg cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga
     1861 agctgcctgt cagtcgggat ggggcggaaa accggatgtg cgggttatcc gcaatctgat
     1921 tacggaaagt gaagtggcgg tggcggggaa cagtaaattc cgcttcgtgg gggctgatgc
     1981 cttctcgcca gacgaactgc gcaccgattt gttcagcgac gacgagggtg gctatgtgga
     2041 ctgcgtggcg ctcgatgccg ccctgctgga aaaactccag gctgtcgcag agttccttcg
     2101 ggaagccgaa ggctgggaat ggtgcgccgg acgcatggag cctgtcggtg agtgccgtga
     2161 ggatgccgga acataccgct gtctgccgga gccggaagcg gtgctgacgg aggcagaaga
     2221 agaacgcctg aacgaactga tggcacgtta cgacgcgctg gaaaaccagt gtgaggaatc
     2281 cgacctgctg gaagcagaaa tgaagctgat tgactgcatg gcgaaggtca gggcgtggac
     2341 gccggagatg cgtgccggaa gtggcgtggt ggtgtcctgg cgttatggaa acgtatgtgt
     2401 ccagcgtggt gtgcagttgc gcagtgaaga tgacgcgact gatgacgctg accgcacgga
     2461 acaggtgcag gagaaagcgt cagtggagga aatcagtctg ccgttgctga cgaaaatgtc
     2521 ttccgaacgc acgctggcag tccaggcagc actgatgcag caaccggaca aatctctggc
     2581 actgctggca tggacgctct gcctgaatgt gtttgacagc ggagcgtaca gtaaaccagc
     2641 acaaatcagc ctggaatgta aacattattc gctgaccagc gatgcgccgt cagggaagga
     2701 aggtgccgca ttcctggcgc tgatggcaga aaaagcccgt cttgcggccc tgttaccgga
     2761 gggatggtca cgggacatga cgacgttcct gtcactcagt caggaggtgc tgttatccct
     2821 gctcagtttc tgcaccgcat gcagccttaa cggtgtccag acccgcgagt gtggtcgcac
     2881 gtcacgcagt ccgcttgact cactggagag cgccatcggt tttcacatgc gcgactggtg
     2941 gcagccgacg aaagcaaact tcttcggaca cctgaaaaag ccgcagatta tcgcagccct
     3001 gaatgatgcc ggactgtccg gtgccgcacg ggacgcagag aagatgaaga aaggcgatgc
     3061 ggctgaacat gcagagttcc atatgaaaga caaccgctgg gtgcctggct ggatgtgttc
     3121 accacgtcca cagacggatg ccactgaacg cgccgataac ctggctgatg ccgcctgatg
     3181 aacaaccaca ccgccccgcc ggagacgggg cggcagaggg agaaatacga tgatgaatac
     3241 cgaactgaca ctgaatgccc tgcagtccat gaatgcccag gaatatgaag aaatccgtgc
     3301 tgcgggaagc gatatgcgcc gtaatctcac tcacgaggtg atgcgtgaag tggacgcacc
     3361 ggctaactgg atgatgaacg gagagtatgg cagcgagttc gggggctttt tccccgtcca
     3421 ggttcgtttc acgccagccc acgaacgttt ccacctggca ttatgttcgc cgggagacgt
     3481 ctctcagctc tggatgctgg ttctggtgaa tggtggtgga cagcctttcg ccgtcgttca
     3541 ggtgcaacat atcttcacgc ctgtcgcgat cagtcacacg ctggcgcttg ccgcgacact
     3601 ggatgcgcag ggatacagtg ttaacgacat catccatatc ctgatggcag aaggaggtca
     3661 ggcatgagcg cacgttcacg ggcactgatc cccctcagcg cagaacaaca ggccgccatg
     3721 caggcggtgg ctgtcacaga acaacgtcgt cgtcagggac gcacactttc agcatggcct
     3781 tatgccaccg ctttctttcg ctgcctgaat ggcagtcgcc ggatttcgct gaccgatctc
     3841 cgcttttttg cccctgcgct gacgaaggag gaatttcatg gcaaccgtct cctgtggctg
     3901 gctgccgtgg ataaactgat tgaaagtttt ggggaagtct gtgttcttcc cctgccatcc
     3961 gatgcggggc accgtctgtt cccgtccgtg ccttttcgtg aaggtgagcg tcgtcgtcag
     4021 aaaaccacgc tgacagagca gaaatacagc cgccagcggg aacgtgaggc agaacgacgg
     4081 gaactggaat accagacatg ttttgctcag gcgcagattg accttgcgtt tcatactccc
     4141 gccacggtcg gaagctggtt gtcccgctgg tctggtgttg ttgaggagca tgatctggaa
     4201 acgattttct gggggtggtg cgggcgtttt ccatcactgt catcatttga ccggtttttc
     4261 tggcaggagg aaccactctg gcggttgatt tttgaagccg gtgaggccgg tcgtggtgca
     4321 ccggtacagg tacgtgcact tgagcagtgg atgatcccga acaagctgga gaacgtaata
     4381 tgatgaaatc agacgaaaaa taccaggttc ccgcgtggat gcgacctctg ttgccgttgc
     4441 tctgcaacac cggggggaac gatccggaag aactgctgaa tgatacagaa accactgcca
     4501 gtgcgaatgt tgtccgttat gtactgatag ttgctgtgcg gtcgcaggtt gatctgctgc
     4561 agcttctgta caggaaaggg ctgttgcgca cagagatacc aggtggcttt tcaccggaag
     4621 aagcgcaggc actcctggat aatctggtgc gcagccatat cagcaaggcg ctgtcgggcg
     4681 agcgaatggc agcccgtgac agaaatgccg atctggcctg gattggacag caactggtcg
     4741 atgccgcctg gtttgtccgt gccacactgg aagcgcatgg gatgagcgtc ggaaatgaga
     4801 gtccccctgc tccgccggag acaatgccgg acatacagac acgggaactg gtgatgttga
     4861 tcaagcgact ggcatcatcg ctgaaagcgg tgaaaccgga cagttgtgtg gtgcgtgaag
     4921 cgcaggactg gctgcgcgac agaaaacttg tggatatcac agatattctc cggtgaacca
     4981 ggcatgtata ttttacgcag ggacatgccg atcatgatcg tttcgttcca ggggtggtct
     5041 ggagatcatg cggtgtgctc cctgctctcg ccggaataag gacgtcgccg ccggctccgc
     5101 ttttacccgg ccatgcgggg catggccttg tgggttttca gttatgtggc ctcagcg
//
LOCUS       DAJTVE010000208         5139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000208 DAJTVE010000000
VERSION     DAJTVE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2216)
                     /locus_tag="OO439_003971"
     CDS             complement(<1..2216)
                     /locus_tag="OO439_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCR6318208.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVP"
     gene            complement(2197..3321)
                     /locus_tag="OO439_003972"
     CDS             complement(2197..3321)
                     /locus_tag="OO439_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HCR6318209.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(3318..4607)
                     /gene="trbA"
                     /locus_tag="OO439_003973"
     CDS             complement(3318..4607)
                     /gene="trbA"
                     /locus_tag="OO439_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCR6318210.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTEKQHPADKEKEQ"
BASE COUNT         1091 a         1293 c         1527 g         1228 t
ORIGIN      
        1 ggtacaggta acagcatctc cagggggagc agagtattca gaatcgcctt cactgaaggt
       61 atatcttcag gaccggagtg ttcctcttcc ggacgattca gtgtgatata acatacccgg
      121 cgttcttccc tggttgtgtt attgatagtg tgcacagcct ttttccacag ttcgtttgcc
      181 cgggattcga tatctgtatc atattgcgga agcagatcca gtgatgtctg cgctgttgcc
      241 aactggcgct ggaatgtatc aatgatacgg taaggctctg tacggttttt acgacgttta
      301 cgggatggtt ttgctgtcag tacaccgatg atgctgctga catgctccgg tgtggggagc
      361 ctgcgctgca ctgtcctggg gacgagacgg cggagacgct ccagccgggg ggcttcaacg
      421 gaaatatagc ggttgatgac caccggatca ctgtcgcaac ttttctcgct gtccggaata
      481 tacacagagg ctgacggcac cacatccccc tggaaaacgg taaagaactc accagcattc
      541 agtctgatca aatcctgcac gtcgattttg ctttccatct ggatggtgat ctggttaccg
      601 tccacccagg actcacccat cacaccatcg tggcgctgca ggctgcccat tctggcccgt
      661 gcctcttcac cggcggcatt tttcaccgtt cttgccgttt tgtcgtcact gacaatgcgt
      721 ccggcgaatt tggtgccggc gttctgcatc agtgtggcgg tgttggtggc ggaggtctgc
      781 ccttcaattc gctcctgatc ctgcgccgtc attatcaggg aaaactccag tgaacgtacc
      841 tgggtggcct ccacagcaat gcggtctgtg tagtaggcac cgacttcgtc aaggatacag
      901 atatagggga agctgccttt gtattttttg acttccaggg tctgtgcatc tgtgccttcc
      961 agccggtagc ccaggtcacg ggcgaggagc atgctctgct gggtgacgaa catacgaccg
     1021 agtgccgacg tggtatgagc cgatgtgtcc agtgcaggga tcatcacaat cagaatgcgg
     1081 tcgctgtgaa tactgtcgcg aatatcgatg tcgccggcat ctgccgcaaa gacgtccccg
     1141 aaggtttcgg caaaggttgt gaacgtttcc gagaactgac cggagagata agcatgctgt
     1201 ttacggggct cctcagtcca ggctgacggc gttctcacca gtgacatatc gaatcccggg
     1261 acatcctgca ggtagtttcg cagtggtgcg atagcttctt ccggccactg gtcatcaagg
     1321 ccacggcaat agagttttgc catcccttcc agcgtcatat gttcccgtaa catctgcagg
     1381 gacatggttt taccttcccg gacgcaccag aattttgtgc caaagaccag ggccttgttc
     1441 atggctatcg cccgtgactg ccattcaccg ccctgaacgt tctggggcag catggactgc
     1501 atggtttcgg cggtgaaggc ttcggtgctg aatgcgaacg ggttccaggt gtttgactgt
     1561 gggcgggatt tttcgccgct ctgaatgatt tcactgcggg attttccgcc gttcatgaag
     1621 ttgatgactt cgatatcatc ctccctgcca aaacgtcggg agagatacca gattgtcctc
     1681 gtggtatcgt tctgtgcctt tccgtcaaca aaggtaaagc ctcttcccca gcagagcggg
     1741 ttgagcagcc aggcaaaggt ggtttctgtt ttaccgccac cggtggtggc aaaaaacatg
     1801 acatgccggg tgaggtcatc catgctcagc cagagctcac gaccgatatc gttgatacgg
     1861 cggtaaccga cataaaaaat cccccgggcg gggaatgttt catccgcttc atactgaaac
     1921 agggtgggcc agaagctgaa gaggcttctc ctgacctttc tgtcctggga ggggtcatca
     1981 aggctgagtg tcatgggcat acgcaggggg catcgccagc gttgcagcat gaatatcacg
     2041 gaggtgacca gggtgaacag caggcagaca ggaagggcca gtggccatat cagcgccgta
     2101 atgatactgc cggccagaca cagaccagtg atgttcaggt tatgcagtgc attccacaac
     2161 gggtttcccc aggccgtgcg gtgtatgagg tcaggattaa cgtggtgctg gctcattgtg
     2221 atactcctcg ctatctgttg ctttcaggaa gaggcgaacg gaagtggcgt ctttcaggat
     2281 gccggacggc aggtgccagc cggtatctga taacacccag ggggttccgc caaaaccgaa
     2341 ctggcggaaa gcctggttat tgacatcgat ggcagcacgt gcggcggtca gacactgtcc
     2401 atcctgtgcg gccggtgcat cgccggtggg ggccatacca ctgtcagcgg aatacagctg
     2461 atgccatccg gtcgcccgtt tttgtgagtc cggttcacac agcagcggag cagtccgctt
     2521 tgtactggac tccccgccaa tcacggagac cggataaatc accacgctga attcccgggc
     2581 cagttgctta agcgcgggtt ccagcagacg gcagttgatg catgcggggt caaggaaggc
     2641 gtacagcgtg cgttcatgac cagtggacag gttaatggtg aacatccccc ggtctgcgtt
     2701 gcgtttaagt actgtggcca gattacggcg ggcctcagcg gcctgaacgt gttgttcttc
     2761 cgtcactgcc gtcggggcgg gggcagaaga aggttcctgc tgtgtgggag ccgggcgggc
     2821 cgggatgctg ttaacatgat gttccgtatg tgggcgtggt gtctctgtgt cggttactgg
     2881 ttcagctctc cattcctgag atgtagggta agccagcgtg gaagggggct ctgccttcat
     2941 ccagtggtaa cctgcaactg tcgccggcag agcaattccg gcgataaagc cacacatcag
     3001 tgtccgccag aagccatttt tccgggaggg atgttttcct tttccggcat attcggcatc
     3061 caggcaggca tcaatcgctg agagcattct gccgatgtga tgtccgggac gttctgcatc
     3121 aacggtatca acaagcaccg tttcggttat ttcgcgaaaa ggggaactga aggacaggtg
     3181 catcagcctg ttattacggt taatcagtgc gcccagttct gtgactggca ggatggcaat
     3241 cacctcccga ttaccgtcag ggtacagggc gatcagggac aggtgtttat tattcagtgt
     3301 tgcagtgtag tggatggtca ttgttctttt tccttgtcgg cgggatgctg tttttcggtg
     3361 gtttcggtgg tttcggtggt ttcggtggtt tcaccagtgt cagtgtcttc gtcgttatca
     3421 tggcggcggg gtatcacgtc atccgtgagt gaccagcggg tcctgatctg acgtttacga
     3481 tcgcgatctg gctgaggttc gtcccatatc aggcccagcc ccagcatgtc ccgtcgaagc
     3541 ccttcaatgg cctcttccac acagggttcc gggcagggga ggccgagacg gctggcttca
     3601 ttttctgccc gggcaacggc caccacgccg gccccttcga tgaagccttt tgtggtgttt
     3661 gcccggtgaa gggcatagaa gagcgtccgg tcaacgccct tgagccacag ccagttcgga
     3721 ggcgtcaggc gcaaatcatg tgcgtaaagc cagaccagac cgctgcgaac gtaacggtgc
     3781 tgcctgagcc actgttgtgc gccttctgtt ttgatgaccc ggataaaggc gtttctggcc
     3841 agtgaataca ccggcgtgct gaaacgcccc tggtcccgtt tacttttgag cctgcaggag
     3901 agattgaggt tgttcatcag ggctaccgct gctttccggt caccgaggaa gaactgcagg
     3961 ccaaagatgg caaagagcgc tttttcatgt ggggccatgt ctttccagct ggtcagtggt
     4021 tgccccagtt gcgccatgaa gcactggcgt gtggacgcaa catcgagctg catgttgcgg
     4081 ataaggttgt tctgctccac aaagacttcg ggggttagtg cgacacggcg ctcgggacgt
     4141 ttttgtccgt ggaagagtct gttgttgtct ccatcagcca gaacgggggc gatcgccggt
     4201 gacagagctg aaaaaatatg gggcagtgtg tggatattca gtggcctgcg ggtaaaacgg
     4261 ggctgggccg gatggtgaaa ccacatccac agaaagccga atgtcatggg aagcagaatc
     4321 aaccagagga tccccactgt ccgggacata acatccagcc actggccgaa agtgatggtt
     4381 gccgcgttgt tagcagtgat ggccagcagg ttgtaacggg gggcggcaaa gttgtgaaag
     4441 ggcccccagt caatcatgcc ccacagtgtg tgcaggatca ggcaggaggc gaagacgatt
     4501 tcaggtaaaa agcgccagat gataatcgca aagagcaggc atatggcacc gacgatatac
     4561 catatagcca ttggatcttc ggcggcagac gtctgttggt aagacacggg gattcctcca
     4621 tcgataaata gataaatttc gggatgaggc tgagtggaat taatgcgaac aatcgccggt
     4681 aatgactggg cagggctgaa ttcagcaggg cattttcaca ggaacagcag aaggtggtga
     4741 gagacaggca caaaaaagcc gactggtgag gccggctttt ttgcgatccc agggagagtg
     4801 ggtggctctg gcccggagga agtctctttt caactaacgt taccagaata tcgttatatt
     4861 cacctgatcg caatctgttc gttacagtct attgttccgg gagtagcgat atcgcctgaa
     4921 acaagtataa tcagcaagtt agccttgtct ggagacttac cttcgcatca atccatcggg
     4981 actatggatc tgtgctattc aaagacctgg ccggacaaac aggtggtgct gcgtctggtg
     5041 ctctggccgg aggaagtctc ttttcagcta acaatgaaag agctgttgtg acaggcaatg
     5101 taagagtaaa caacagtgat ttcctccatt gatggagaa
//
LOCUS       DAJTVE010000209         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000209 DAJTVE010000000
VERSION     DAJTVE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..379
                     /locus_tag="OO439_003974"
     CDS             <1..379
                     /locus_tag="OO439_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318211.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OO439_003975"
     CDS             complement(575..2125)
                     /locus_tag="OO439_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6318212.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OO439_003976"
     CDS             2297..2743
                     /locus_tag="OO439_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6318213.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OO439_003977"
     CDS             complement(2937..3872)
                     /locus_tag="OO439_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6318214.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OO439_003978"
     CDS             4384..>5114
                     /locus_tag="OO439_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318215.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1126 g         1327 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgcctga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJTVE010000210         5101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000210 DAJTVE010000000
VERSION     DAJTVE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..516
                     /locus_tag="OO439_003979"
     CDS             <1..516
                     /locus_tag="OO439_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCR6318216.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
     gene            complement(713..2650)
                     /locus_tag="OO439_003980"
     CDS             complement(713..2650)
                     /locus_tag="OO439_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6318217.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            3061..3768
                     /locus_tag="OO439_003981"
     CDS             3061..3768
                     /locus_tag="OO439_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6318218.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3874..3949
                     /locus_tag="OO439_003982"
     tRNA            3874..3949
                     /locus_tag="OO439_003982"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3907..3909,aa:Phe,seq:gaa)
     gene            4147..>5101
                     /locus_tag="OO439_003983"
     CDS             4147..>5101
                     /locus_tag="OO439_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6318219.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1346 a         1322 c         1242 g         1191 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgacgt agcccggtac aagagtaacc cacgttagcg cttggcgacg gcggaacaaa
      601 gctgattcgc gacgtaagaa agtggcaggc tcatatgacg gacctctgta atgcggtatc
      661 caacccgcga atatcagcgt gattcaccgt cggatttact gctacgtcgc cctcagggaa
      721 ttaaacctaa aaagaaaatc gtcaaaatta gagattccgg tttgacacgg aaagtcatat
      781 cagcgctatg cgcatcctgg gggttcatca ccactgacgg gaaactctgc tggcggaaca
      841 tcgctttttg taggtctttg acgtcgaaac tgacatacag atcgtgcatc tcctgacaca
      901 actggcgcag ggtgtagtcg cgatagcgca ccggatattt gttataaacg ctcggcaaca
      961 cctcagccag cggcgagtca tcctcaatat gctgttcaaa ttgcgccagc atcgccacca
     1021 gttgtgccag cttctcgtgg ctttccgccg gagttaataa gaacagaatg gagttgagat
     1081 cgcacttctc cggcacaatg ccgttctcac gcagatagtg cgccagaatc gtcgccggaa
     1141 cgccaaagtc gctatattcg ccggtttcgg cattgatacc tggtgtagtg agtaacagct
     1201 tgcacggatc aacaaaatac tgatccgcgg catatccttc aaagccgtgc cacttcgccc
     1261 ccggctcaaa actgaaaaaa cggcggtcgc tggctaacac agatgtcgga taatcctgcc
     1321 acaatttgcc atcaacaacg ggcgggataa acgggcggaa cagcttacag cgcgcaagaa
     1381 tagccttgcg cgactcaata cccaactcaa cgcactcagc ccacagccga cgcccactct
     1441 ccccttcatg aattttggcg ttaacatcca gtgcagcaaa cagcggatag aaagggctgg
     1501 tagaagcatg gagcataaag gcgttattca accgcttatg cgggcaaaaa cgcgcctgtc
     1561 cgcggatatg gttatctttt ttatggatct gcgacgtctg tgagaatccc gcctgctgtt
     1621 tgtgcaccga ctgagtcaca aagatccccg gatcgttttc gttaagttct aatagcagcg
     1681 gcgagctatc cgccatcatc gggataaact gttcgtaacc gacccacgcg gaatcaaaca
     1741 gaatgtaatc acacagatgc ccaacggtat cgatcacctg acgggcgtta tagacagttc
     1801 cgtcataggt tcccagctga ataatcgcca ggcgaaacgg gcgcggcagc tcggcttttt
     1861 ctggcgcaac gtcgcgaatt tgctggcgca gatactcttc attaaaacag tgcgcatcaa
     1921 taccgccaat gaaaccaaac gggttgcgtg aagcttccag atagaccgac gtcgcccccg
     1981 cctgaatcag cgcgccgtga tgattcgact tatggttgtt acggtcgaag agcaccagat
     2041 cgccacgcgt taacagcgca ttcgtcacca ctttattcgc tgccgatgtg ccgttcagca
     2101 caaaataggt tttatcggca tgaaagactt tggctgcgaa tttctgcgca tccttcgccg
     2161 atccttcatg aataagcaga tcgcccaatt ttacgtcagc gttacacata tcggcgcgaa
     2221 agacgttctc accaaagaaa tcgtaaaaat ggcgtccggc aggatgcttt ttaaaaaacg
     2281 caccatgttg atgtccaggg caagcaaagg tgctgttgcc catctcaacg tactgcgtca
     2341 gcgtgtcata aaacggtggc agcaaattct cttcatactg acaggctgcg gattccagct
     2401 ccagccactg ctgctcgttg ccgttgatta ccgccgtaac gcccgcaggt aattcaacag
     2461 catgttcgga atacaaaaac accggtagat gaaaaccggt gcgcttaagc aacgcaagaa
     2521 tgccactgcg actatccgca gcggtaatga cgactgccgc gacatccgta aaatcagtat
     2581 ctcccaacgc caccacgcgg cgatgagtag aaagtcggga taccagttca ctactggcgg
     2641 caatattcat tgatttcata agcgcaaaac ccgtttcggg gaagtaagaa taccggacaa
     2701 agtgtaaaac cctgccccat gagatatggg tcaaactggt caccagctcc gacgaccgcc
     2761 agacatcagt aaaagcagaa acgctctgat tttactgttg tcctgcagtg aacgtgcgtt
     2821 aacttcaccg catgagcagt aacatagaaa gggaaacgtt tcgcgcgaaa cggcgataag
     2881 cgagagaatg taaggagatg gcgtgcatcg ggcaaactcc gtacagagag gagaaaattc
     2941 gcgcaatcat ggcacctttc gctaaggcgt gcaagccagg actttgcgac cagacaagcc
     3001 atcaacaagg caaaaagttc ataataagaa aatcaaacaa tacagctaac aggaactctt
     3061 gtggtcatcg gcccttttat caacgctagt gcagtcttac tgggtggcgt tctcggcgca
     3121 ctgctcagcc aacgcttacc ggaacgtatc cgcgtctcca tgacatctat ttttggtctg
     3181 gcatcgctgg ggattggtat tttactggtt gtgaaatgcg ccaaccttcc agcgatggtt
     3241 ttagctacgc tacttggcgc tctaattggc gaaatttgtt tgctggagaa aggtgtcaat
     3301 acagcagtcg ccaaagcaca aaatctgttt cgtcactcac gtaagaagcc agcgcatgaa
     3361 tcttttattc agaattatgt cgcgattatt gtcctgtttt gcgccagcgg caccgggatc
     3421 ttcggggcga tgaacgaagg gatgaccggc gatccgagta ttttaatcgc caagtcattt
     3481 cttgatttct ttacggcgat gatcttcgcc tgctcgctgg gtattgcggt gtcggtaatt
     3541 agtatcccat tactgatcat ccagctaacg ctagcgtggg ctgccgcgct gatattaccg
     3601 ctgaccacac cgtcgatgat ggcagacttc agcgccgtag gcggtttatt gctgctggca
     3661 accggattac gcatctgtgg cattaaaatg ttcccggtgg tcaacatgct tccagcactc
     3721 cttctggcaa tgccgctttc cgccgcctgg accgcctggt ttgcctgaca atgcgtgcaa
     3781 tagcggcaaa gtgatgatag attgtgcagt ctgcagtaaa ttgaagaaat ttgattgacg
     3841 agacgaggcg aatcaggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
     3901 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
     3961 acggacctcc acggaggtcc gtttttcgtt tcagaacgcc acgatttaag cgttctgcct
     4021 acaaatcaat tctaccgaac tcaaccagat tccccccaca tcaaccccat tgtgtgggta
     4081 taattgcggg cataccccag ttcgacagaa tttgtaccct ctttaacgca ttcagaaggc
     4141 tgaactatgg cactgactga cgcaaaaatc cgggctgcaa agcccactga caaggcttat
     4201 aaactcactg acggggctgg catgttcctg ctggtacatc ctaatggctc ccgttactgg
     4261 cgtctccgtt atcgtattct gggtaaggag aagactctgg cacttggtgt gtatccagaa
     4321 gtttctctct ccgaagctcg tacaaaacgg gatgaggccc gaaaactgat ttcggagggg
     4381 gttgaccctt gcgaacagaa aagagctaaa aaagtagtcc ctgatttaca gctctctttt
     4441 gaacatattg cgcgacgctg gcatgccagt aataaacaat gggcacaatc acacagcaat
     4501 aaagtactca aaagccttga gactcacgtt ttccccttta tcggcaaccg ggatatcaca
     4561 acactcaata ccccggatct gcttatccct gttcgtgctg cagaagctaa acaaatttat
     4621 gaaatcgcca gtcgtctgca gcaaagaata tctgccgtaa tgcgttatgc cgtacagtct
     4681 ggcatcatca gatataatcc tgctctggat atggctggcg cattgactac ggtaaaacgc
     4741 cagcatcgcc ccgctcttga tctttctcgc ctgcctgaac ttttgtcgcg tattggcagt
     4801 tacaaagggc aacctgtcac ccagcttgcc gttatgctga atttactggt ttttattcgt
     4861 tccagtgaac tcagatacgc ccgctggtct gaaattgata ttgacaatgc catgtggact
     4921 attccagccg aacgcgaacc cctgccaggc gtaaaattct cacaccgggg ctccaagatg
     4981 cgaacaccac atcttgtgcc actcagcaaa caggctgtag ccatactgac agaactacag
     5041 acatgggctg gtgaaaatgg tctgatcttt acgggagcac atgacccgcg taaaccaatc
     5101 a
//
LOCUS       DAJTVE010000211         4904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000211 DAJTVE010000000
VERSION     DAJTVE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1471
                     /gene="lsrA"
                     /locus_tag="OO439_003984"
                     /pseudo
     CDS             1..1471
                     /gene="lsrA"
                     /locus_tag="OO439_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1465..1773
                     /locus_tag="OO439_003985"
     CDS             1465..1773
                     /locus_tag="OO439_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6318220.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(1945..3393)
                     /gene="uxaB"
                     /locus_tag="OO439_003986"
     CDS             complement(1945..3393)
                     /gene="uxaB"
                     /locus_tag="OO439_003986"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6318221.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3620..4570)
                     /locus_tag="OO439_003987"
     CDS             complement(3620..4570)
                     /locus_tag="OO439_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6318222.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4709..>4904)
                     /locus_tag="OO439_003988"
     CDS             complement(4709..>4904)
                     /locus_tag="OO439_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6318223.1"
                     /translation="ANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEE
                     QQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1269 a         1257 c         1199 g         1179 t
ORIGIN      
        1 agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg tccacgccct
       61 gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg gtattacccc
      121 tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa cgccagttca
      181 tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc caagcctgtc
      241 gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc agaaaatgaa
      301 gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag gatcgctgga
      361 tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact cgcggattct
      421 gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct tgtttcgtcg
      481 cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata agctgccgga
      541 aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg ccttaagcgg
      601 caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag tggtacggga
      661 aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc gcccacaaca
      721 tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt tcaggaatgt
      781 cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg gggccggacg
      841 cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac gcattatgct
      901 gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg gtctggttta
      961 tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg cctggaacgt
     1021 ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag ataatgccac
     1081 cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac aagctgcacg
     1141 gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg aagcttcgcc
     1201 gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc gtaatgatat
     1261 ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta tctcctctga
     1321 tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg gcgaaattac
     1381 ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg ttgccttcgg
     1441 cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg tgaaatcacg
     1501 gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg ccagtattta
     1561 agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct gctggcaatg
     1621 ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc ttggataata
     1681 ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg
     1741 ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac
     1801 gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt ggcatctgtt
     1861 aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac ccggtcaaaa
     1921 ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc gcatcccttt
     1981 ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca cttgcgtcag
     2041 gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct gggtaccgat
     2101 aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt gtgcatcgtc
     2161 ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca atgccgctaa
     2221 tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca gcagctgcgg
     2281 caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca gctgatgctt
     2341 atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt catcacgagg
     2401 caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg cgcaaatttc
     2461 agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca ccggcaccag
     2521 cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt taatatcgtc
     2581 aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta aggatttcgg
     2641 cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat cgtgataacc
     2701 cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga tacggtccac
     2761 cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt ctggcagtgc
     2821 ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt agtcgatcaa
     2881 ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc taaagcgttc
     2941 gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat cgaatttatc
     3001 gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc gcatttccgg
     3061 gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt cacgattaac
     3121 cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc cacggataat
     3181 ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt caatcggacg
     3241 aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga tttgccagtc
     3301 aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg gatactgtgc
     3361 accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga ttagtcatac
     3421 aacctgtttt aattggtacg acaggttagc aaactttaat acgccgaacc cctgttttga
     3481 tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga tcatggttta
     3541 atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc attcaaatcg
     3601 cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg ctggtacggt
     3661 tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc aatttattaa
     3721 aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata cttaccgtga
     3781 gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta cggatttttt
     3841 ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca aattcttcgc
     3901 cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc cgggcaaaca
     3961 cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt ttgaaatagt
     4021 cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag aattgctcac
     4081 tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc gaaccaatcg
     4141 ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc cgtaaggcac
     4201 ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt aaggcgacgg
     4261 taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc cattcggtgg
     4321 ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc agtcgattaa
     4381 gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag aaaagagaaa
     4441 gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt agcgcaaaaa
     4501 tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt gaaatggggt
     4561 gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca acgtaataag
     4621 taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag gtaccctggg
     4681 aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca accagccagt
     4741 gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca ccctgcggaa
     4801 tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc tgagcgataa
     4861 acaccggatg cccgcgcatg ggggtttgct taccgtcatt agcc
//
LOCUS       DAJTVE010000212         4890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000212 DAJTVE010000000
VERSION     DAJTVE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            72..187
                     /gene="rrf"
                     /locus_tag="OO439_003989"
     rRNA            72..187
                     /gene="rrf"
                     /locus_tag="OO439_003989"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(305..748)
                     /locus_tag="OO439_003990"
     CDS             complement(305..748)
                     /locus_tag="OO439_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6318224.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            905..1834
                     /gene="metA"
                     /locus_tag="OO439_003991"
     CDS             905..1834
                     /gene="metA"
                     /locus_tag="OO439_003991"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6318225.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2103..3704
                     /gene="aceB"
                     /locus_tag="OO439_003992"
                     /pseudo
     CDS             2103..3704
                     /gene="aceB"
                     /locus_tag="OO439_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3734..4843
                     /gene="aceA"
                     /locus_tag="OO439_003993"
                     /pseudo
     CDS             3734..4843
                     /gene="aceA"
                     /locus_tag="OO439_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1225 a         1280 c         1250 g         1135 t
ORIGIN      
        1 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
       61 aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg ccgaactcag
      121 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      181 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc attggcgcag
      241 aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc acggcagggg
      301 cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc aaatcgtcca
      361 cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc gcctgctcat
      421 tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc tccaccagca
      481 tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc atatgctgcc
      541 cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc cacaacggcg
      601 cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct gctgataaaa
      661 aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc ccttcctcat
      721 gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct tatattctaa
      781 cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat tggcaaattt
      841 tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt gaggtaatca
      901 ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc gtgaagaaaa
      961 cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac ttaaggttct
     1021 gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc gcctgctttc
     1081 aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg aatcgcgcaa
     1141 cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc aggaacaaaa
     1201 ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta atgatgtcgc
     1261 ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca cctcgacgct
     1321 gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc ctaagcaaac
     1381 tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc atgcgcttct
     1441 gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg actttccggc
     1501 agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag aaggggatgc
     1561 atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc ccgaatatga
     1621 tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag acccggatgt
     1681 accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga gctggcgtag
     1741 tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga tcacgccata
     1801 cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg atcgttaagc
     1861 gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa acgcacctca
     1921 gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt tttaattaaa
     1981 atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc tgaacgaaaa
     2041 gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg aggagctgca
     2101 cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg tatggcgagc
     2161 aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg gtgacgcatt
     2221 ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa gatattgata
     2281 acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct gattggaaaa
     2341 ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc ccggtagagc
     2401 gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc gatttcgaag
     2461 attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg cgtgatgcag
     2521 ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc aagcccaatc
     2581 cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat gtcacctggc
     2641 gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc cacaactatc
     2701 aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc cagtcctggc
     2761 aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt aatctgccgc
     2821 gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc cagatggacg
     2881 aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc tgggattaca
     2941 tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca gacagacagg
     3001 ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt aaaacctgcc
     3061 ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc aaagatgaag
     3121 agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa gtcaataacg
     3181 gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg gcggtattca
     3241 acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa gacgcgccga
     3301 ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa ggtatgcgcg
     3361 ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac ggctgcgtgc
     3421 cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc tcgatctggc
     3481 agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa gccttgttcc
     3541 gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa gaacgtttct
     3601 cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact tccgatgagt
     3661 taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc acataactat
     3721 ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga aagagtggac
     3781 tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg tgaaattacg
     3841 cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa tgtggcgtct
     3901 gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga ctggcggtca
     3961 ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat ggcaggtagc
     4021 ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc cggcaaactc
     4081 ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc agatccaatg
     4141 gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc cgatcgttgc
     4201 cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga aagcgatgat
     4261 tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga agaaatgcgg
     4321 tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac tggtcgcggc
     4381 gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta ccgatgccga
     4441 tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta ttaccggcga
     4501 gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga tcagccgtgg
     4561 cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc cggatctgga
     4621 actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac tgctggctta
     4681 taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta ttgccagctt
     4741 ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg caggtatcca
     4801 cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg agggtatgaa
     4861 gcactacgtt gagaaagtgc agcagccgga
//
LOCUS       DAJTVE010000213         4804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000213 DAJTVE010000000
VERSION     DAJTVE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..1322)
                     /locus_tag="OO439_003994"
     CDS             complement(279..1322)
                     /locus_tag="OO439_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6318226.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OO439_003995"
     CDS             complement(1734..2918)
                     /locus_tag="OO439_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6318227.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OO439_003996"
     tRNA            complement(3218..3312)
                     /locus_tag="OO439_003996"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4804
                     /locus_tag="OO439_003997"
     CDS             3605..>4804
                     /locus_tag="OO439_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6318228.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1056 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgcc
//
LOCUS       DAJTVE010000214         4796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000214 DAJTVE010000000
VERSION     DAJTVE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..81
                     /locus_tag="OO439_003998"
     tRNA            6..81
                     /locus_tag="OO439_003998"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO439_003999"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO439_003999"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO439_004000"
     CDS             complement(299..1057)
                     /locus_tag="OO439_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6318229.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO439_004001"
     CDS             complement(1065..2168)
                     /locus_tag="OO439_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6318230.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWQINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OO439_004002"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OO439_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="OO439_004003"
     CDS             complement(3427..4452)
                     /locus_tag="OO439_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6318231.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1237 c         1146 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa tttgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAJTVE010000215         4790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000215 DAJTVE010000000
VERSION     DAJTVE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            232..573
                     /gene="raiA"
                     /locus_tag="OO439_004004"
     CDS             232..573
                     /gene="raiA"
                     /locus_tag="OO439_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6318232.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    676..801
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            677..724
                     /gene="pheL"
                     /locus_tag="OO439_004005"
     CDS             677..724
                     /gene="pheL"
                     /locus_tag="OO439_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6318233.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            824..1984
                     /gene="pheA"
                     /locus_tag="OO439_004006"
     CDS             824..1984
                     /gene="pheA"
                     /locus_tag="OO439_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6318234.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2027..3148)
                     /gene="tyrA"
                     /locus_tag="OO439_004007"
     CDS             complement(2027..3148)
                     /gene="tyrA"
                     /locus_tag="OO439_004007"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6318235.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3159..4229)
                     /gene="aroF"
                     /locus_tag="OO439_004008"
     CDS             complement(3159..4229)
                     /gene="aroF"
                     /locus_tag="OO439_004008"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6318236.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4438..>4790
                     /locus_tag="OO439_004009"
     CDS             4438..>4790
                     /locus_tag="OO439_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6318237.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1310 a         1226 c         1093 g         1161 t
ORIGIN      
        1 gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga tctcatcaaa
       61 tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg atttctcaca
      121 aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac attttgacat
      181 caggaacggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt tatgacaatg
      241 aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt cgcagaccgt
      301 ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat tctgtccaaa
      361 gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt tctggttgcc
      421 agtggtaaac atgaagatat gtacactgca attaacgaat tgatcaacaa gctggaacgg
      481 cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc ggtgaaagac
      541 gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgg atcgccaacg
      601 cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg tactaaagtc
      661 acttaaggaa acaaacatga aacacacacc gtttttcttc gcattctttt ttaccttccc
      721 ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat aaggcctccc
      781 aaaccggggg gcctttttta ttgataacaa aaaaggcaac actatgacat cggaaaatcc
      841 attactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc
      901 agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
      961 acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca
     1021 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     1081 aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact cagcacgcat
     1141 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     1201 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     1261 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     1321 aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     1381 aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca ttaatacggt
     1441 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atcctcactg
     1501 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     1561 accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     1621 ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt tggcacgtaa
     1681 agccattaac atgtctgacc aggttccggc gaaaacgacg ttgttaatgg cgaccgggca
     1741 acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc tgattatgac
     1801 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatatttt atctggatat
     1861 tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaattac
     1921 ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg ttgatccaac
     1981 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcggttgt
     2041 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     2101 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     2161 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     2221 acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc ccgaccatcg
     2281 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     2341 cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc tgaataaacg
     2401 ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc cagacctgaa
     2461 tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag accacaactt
     2521 gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt accgggccat
     2581 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     2641 tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg tggattggca
     2701 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     2761 gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac aggcgtccca
     2821 tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt gttttaaatc
     2881 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     2941 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     3001 cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa
     3061 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     3121 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcagc
     3181 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     3241 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc agaagactga
     3301 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt gccatctttg
     3361 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     3421 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     3481 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatgacatgc
     3541 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     3601 aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     3661 gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     3721 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     3781 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc
     3841 aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg
     3901 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     3961 agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga
     4021 gcagtttccg gatcatgaat ggaacaagga ccacaaacta ccagcagacg aggatcgcgc
     4081 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     4141 ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc gtcggtaata
     4201 tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat
     4261 agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     4321 tttcgtacat ttttctttac acgaacaatt tgctcgcgct tttgatgctt atttcgtaca
     4381 aataaattta cacctccttt aaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg
     4441 atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca gattgatcct
     4501 tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat ggaagatgcg
     4561 atatcgggca gcgccataaa agatgacgat gcatttagcg attcacaggc ggatcgcggt
     4621 ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga ttttacttat
     4681 gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga aagtgccagc
     4741 caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
//
LOCUS       DAJTVE010000216         4700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000216 DAJTVE010000000
VERSION     DAJTVE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            387..1469
                     /locus_tag="OO439_004010"
     CDS             387..1469
                     /locus_tag="OO439_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6318238.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO439_004011"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO439_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6318239.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OO439_004012"
     CDS             complement(2127..2267)
                     /locus_tag="OO439_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6318240.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO439_004013"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO439_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6318241.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OO439_004014"
     CDS             complement(2623..2913)
                     /locus_tag="OO439_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6318242.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OO439_004015"
     CDS             complement(2906..3076)
                     /locus_tag="OO439_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318243.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OO439_004016"
     CDS             complement(3076..3531)
                     /locus_tag="OO439_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6318244.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="OO439_004017"
     CDS             3796..4173
                     /locus_tag="OO439_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318245.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1351 a         1048 c          978 g         1323 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgccttc catttaaaat gggctaagac ctataaccct aaatattatt
     4681 ctttattatc ttctttacca
//
LOCUS       DAJTVE010000217         4609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000217 DAJTVE010000000
VERSION     DAJTVE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2164
                     /locus_tag="OO439_004018"
     CDS             <1..2164
                     /locus_tag="OO439_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6318246.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2398..3681)
                     /locus_tag="OO439_004019"
     CDS             complement(2398..3681)
                     /locus_tag="OO439_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6318247.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3816..4508)
                     /locus_tag="OO439_004020"
     CDS             complement(3816..4508)
                     /locus_tag="OO439_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6318248.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1096 a         1142 c         1193 g         1178 t
ORIGIN      
        1 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
       61 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
      121 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
      181 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
      241 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
      301 tatgcgtgag atgatggcag gcgggggcaa aatgatcctc gggtcagaca gccacacccg
      361 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
      421 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
      481 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
      541 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
      601 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
      661 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta
      721 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
      781 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
      841 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
      901 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
      961 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     1021 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt acccgtcatc
     1081 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     1141 cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata cgccaatcaa
     1201 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     1261 agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     1321 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     1381 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     1441 aactcagcaa ccgctgattt acgggccgaa cattaaagac tggccggaat tgggtgcact
     1501 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga ccaccaccga
     1561 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcgga
     1621 gtttaccctg tctcgccgtg atcccggtta tgttggcaga agtaaagcga ctgctgagct
     1681 ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa
     1741 gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca gcatggtcta
     1801 tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat
     1861 tggcggtctg gcgaatattg ccgaggagta cgcgactaaa cgctaccgtt ctaacgtcat
     1921 caactggggg atgttacctc tgcagatggc ggaagtgcca acctttgaag tgggggatta
     1981 catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta
     2041 tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa gtctgactgc
     2101 tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat
     2161 gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgcttg
     2221 cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccagataagg catttacgca
     2281 gcatccggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct acaaatcgca
     2341 tcgaaccgta ggccggataa ggcgtttacg ccgcatccgg caaatagtta attgctctta
     2401 cttcttcgcc tctgcaacca ctttactacc cacgccgcgg ttattgtatt cccacatgcg
     2461 gttgtagtta gtgtcattca gattgcgctg tatttcgtcg ttatcatcta cgctgccggt
     2521 attacccgca aacggacgat tagagatcac cgcatcggcc cacggtttag ccgtgttaaa
     2581 accttcgttg atggcgctat cacggatcac cacctgaccg ttggtattgg catcaacatc
     2641 cagcgagcgg cccagttgcg ccacaccatc accgaaagca ttgaaacggc tgtttacggc
     2701 gaggaaaccg tagtacatgt tggacagcgt agccggtgca aacacatacg cttcttgctg
     2761 agtacgtgag ttcaccacgc ggaattcggt gttatcgaac accactgcgc cgcgaccaga
     2821 aacgatatcc acatcccctt caatgtagct gttggtcacc agcgtacgcg gctgacgatt
     2881 cgtttccaga cggttctgca caccgctgtt ggtgacaaag aaggtgttct gacgaccgag
     2941 aatgttaacg ttgttaatct gtacctggtc accatcagta cgcagtgcca ccgccggatg
     3001 gttacctgca tctacgctat cgcccagcgt gttttcgatg gtcagatttt gcagttgcag
     3061 gccattgttt tgtgaccaga agaccgcaga gcagagaaca ccgatactgt cgctgcgttt
     3121 gctctggcag ctatcgtaca tataccacgc tggtttacct ggcatatatt tgccgcgcgg
     3181 gttgacgtcg tgacgccagt cggcagggct catgccacca tcaagggaaa gcccaatctt
     3241 cacatcaatc ggtttttcac ctgtaccgta cagagtaatt ccacccggag cggcagggac
     3301 atataccgtt ccctgatact caccaggcat cacggcaata tactggcgct tgttggtacg
     3361 cttgataatt gccgcatcta ccgccgcctg aatcgtggta tgcgttacac cttgagtgcc
     3421 cgccgggccg acaacaaagt caggttgcgc aggcagggta atcggggaag gattccacgc
     3481 tgcagcacct ggtgtcaggg atgcaaaata gtgttgagca tcgaaattct gcgcttcttt
     3541 tgccgacaga atcgggcgag aagaggtacc aggcgcggtt tgatcagaag gacgttgatc
     3601 gggcggggtt gagctacagg cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg
     3661 ggaaactgaa aatgtgttca caggttgctc cgggctatga aatagaaaaa tgaatccgtt
     3721 gaagcctgct tttttatact aagttggcat tataaaaaag catcgcttat caatttgttg
     3781 caacgaacag gtcattatca gtcaaaataa aatcattatt tgatttcaat tttgtcccac
     3841 tccctgcctc tgtcatcacg atactgtgat gccatggtgt ccgacttgtg cccgagaaga
     3901 tgttgagcaa acttatcgct tatctgcttc tcatagagtc ttgcagacaa actgcgcaac
     3961 tcgtgaaagg taggtggatc cccttcgaag gaaagacctg atgcttttcg tgcgcgcata
     4021 aaataccttg atactgtgcc ggatgaaagc ggttcacgac gagtagatgc aattatggtt
     4081 tctccgccaa gaatctcttt gcatttatca agtgtttcct tcattgatat cccgagagca
     4141 tcaacatgca atgttgtagg gatggcaatt tttacgcctg ttttgctttg ctcgacataa
     4201 agatatccat ctacgatatc agaccacttc atttcgcata aatcaccaac tcgttgcccg
     4261 gtaacaacag ccagttccat tgcaagtctg agccaacatg gtgatgattc tgctgtttga
     4321 taaattttca ggtattcgtc agccgtaagt cttgatctcc ttacctctga ttttgctgcg
     4381 cgagtggcag cgaccggttt tgttgttata tggccttcag ctattgcctc tcggaatgca
     4441 tcgctcagtg ttgatctgat taacttggct gatgccgcct tgccctcgtc tatgtatcca
     4501 ttgagcattg ccgcaatttc ttttgtggtg atgtcttcaa gtggagcatc aggcagcccc
     4561 ctccttattg ctttaatttt gctcatgtaa tttatgagtg tcttctgct
//
LOCUS       DAJTVE010000218         4599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000218 DAJTVE010000000
VERSION     DAJTVE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..853)
                     /gene="mhpB"
                     /locus_tag="OO439_004021"
     CDS             complement(<1..853)
                     /gene="mhpB"
                     /locus_tag="OO439_004021"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCR6318249.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISA"
     gene            complement(855..2519)
                     /locus_tag="OO439_004022"
     CDS             complement(855..2519)
                     /locus_tag="OO439_004022"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCR6318250.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            2561..3394
                     /gene="mhpR"
                     /locus_tag="OO439_004023"
                     /pseudo
     CDS             2561..3394
                     /gene="mhpR"
                     /locus_tag="OO439_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3471..4553
                     /gene="lacI"
                     /locus_tag="OO439_004024"
     CDS             3471..4553
                     /gene="lacI"
                     /locus_tag="OO439_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCR6318251.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT         1064 a         1319 c         1267 g          949 t
ORIGIN      
        1 atgcagaaat agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc gactttccgg
       61 caatggcgga aagctcttcg ttactgacgg catccagttc ctgtatgcgt ccctgctcca
      121 gcaaagtcat gaactggtta tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct
      181 caacaaactt ctcagcggcg ctaatcaccc tttgctgacg caattcgcgc tcactggcgg
      241 gtaaatcttt cccgctcccc aacagacggt cgcgcatatg ggcatcggct ttcgccagtt
      301 cgggcaccgg cggctgatgg gaaagcccac cggaacccag gaacagcacg cgtttattga
      361 gagtgctggt gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg aaaccgggca
      421 gcggcgtggc gacaccgttg atgaacacag gcagaactgg caccttatcc agcccaccga
      481 gcaggaactc cagcggctgg gcgaacccgt ggtccacctg catacagtaa gaaacggcaa
      541 gatcgatccc gctcttcatg acagcatgcg cacaggcctc cgccagctcc acaggcacgg
      601 gcagctctcc tgccgcactg ccgaaatcac caattgccgt cgctccaacg cctaaacaga
      661 acggtggcat cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca
      721 gttcagggga gaaagccgca atacgctcgc gggcgctggc aatcacgcca ttgacctcat
      781 cgagtacctc ttgcgccggg tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa
      841 gataagcgtg catatcaggc taccttttcg acagaaacgt cggcatcagg gcgggtcagc
      901 gtcatcaccg acgccagttt attcagcgta ttgcccaggg tttgcggaat ggcggtggcg
      961 gcaacaaagc gatccgggcg catcaccacc agcgaagcat tgtgttgcgc gaaccagcta
     1021 cgcagacgac cttgcgtatc gcccacgcgt agcacgccgt cgtggttatc ctgtgcggta
     1081 tgaatttgca cttccggcac cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg
     1141 atttgctcgt cgctcatccc ccacagtgga ttgcatcccc agccaattac cgcgaagttc
     1201 gcgccgatcg cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga
     1261 ataaacatct tgccgaccgg agagtgcttc gcttcgccct cacgcaccag cgcaccgccg
     1321 taatattgcg gcatcggctt gaagcgcatt tcgaggaagt agcgttttac tggcggcaga
     1381 taattcaaca gccaggaaac gccgtcacgt aacgtaccct gccagcgttt cggcggagcc
     1441 agcacgttgc ccgccgtcac ggacaggtca atcatcgctt tggcgtgatc gcgacgttct
     1501 tgttgatagg tatcgagcag cgcatcgcgg gctttcccct ggataaccaa cgccagtttc
     1561 caggcgaggt taaaggcgtc gcgcatacca ctgttatagc cctgcccctg ccacaccggc
     1621 atgatgtgcg cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt
     1681 cgcgcgttat gggtgtagac acgctggcga atcaattcaa cattgtccgg attaggcagc
     1741 actttgctta acagcttgcg catattttgc ggctcacgca gctgctcttc ggtttctccc
     1801 ggcatcacca taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga aacatacggg
     1861 cgcaccggat cgcaacacaa atagatatgc ggcgtactta acggatcgtt ggcgatatct
     1921 accacaatcc actgatttgg cgcagtttta ccttcaaacg gcacattcag ggtgcgacga
     1981 acaaaacttg ccccaccatc acaggccacc agccactggg ctttgatcgt ttcccgctgc
     2041 ccttctgccg ttttcaggtg caaggtcact tcgtcatctt gctggctgaa ggcctccagc
     2101 tcgcgggaaa acaagcagcg cacattcgga aaacgcgaca acccttccag catcaccgca
     2161 tcgacctgtg gctgaataaa ggcgttacgg cgcggccagc caaattcatc ggtcattggc
     2221 tgaatatcag caaaacagcg gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta
     2281 gtgtgcggca gaacattctc gaccaggccg accgactgca tggtgcgcag cgcctcgtca
     2341 tcaataccaa tcgcacgcgg gtagtcgatc aacttatcga gtttctccac caccagcacg
     2401 tcaatgccca tctgaccgag atagttcgcc atcatcagcc caaccggacc ggcaccagcg
     2461 atcgccacct gaacgctatg gttaacagca ggctggatgt cagggtgttg tattgccatt
     2521 tctgcacaac gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac
     2581 ggaatacaaa accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa
     2641 acttgatggc ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac
     2701 tgtgcggcga ctgctggaga cgctgcagga agagggatat gtccgccgta gcccctccga
     2761 tgacagtttt cgtctgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca
     2821 gtggatttct gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag tggtatgacc
     2881 aacagatgtg tccaccctgg atgttgatgc aatggtggta cgcgaaacca cccaccgttt
     2941 cagccgctta tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc
     3001 ctcgggcctg acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat
     3061 gttagccgcc cgccccggtg atgactatca actggcacga gaaccgttaa agctgcaagc
     3121 cattctggcg cgcgcgcgca aagagggtta cggacagaac taccgcggct gggatcagga
     3181 ggagaagatc gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct
     3241 gaatctggtg tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc
     3301 ggcgctacaa cgggtagcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt
     3361 ggccggacgg cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc aaaacctttc
     3421 gtggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     3481 taacgctata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg
     3541 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     3601 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     3661 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     3721 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     3781 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     3841 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     3901 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     3961 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     4021 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     4081 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     4141 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     4201 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     4261 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     4321 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     4381 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     4441 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4501 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4561 gcaattaatg tgagttagct cactcattag gcaccccag
//
LOCUS       DAJTVE010000219         4413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000219 DAJTVE010000000
VERSION     DAJTVE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1137)
                     /locus_tag="OO439_004025"
                     /pseudo
     CDS             complement(1..1137)
                     /locus_tag="OO439_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1137..1319)
                     /locus_tag="OO439_004026"
     CDS             complement(1137..1319)
                     /locus_tag="OO439_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318252.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1331..3064)
                     /locus_tag="OO439_004027"
     CDS             complement(1331..3064)
                     /locus_tag="OO439_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6318253.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3061..3555)
                     /locus_tag="OO439_004028"
     CDS             complement(3061..3555)
                     /locus_tag="OO439_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6318254.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3677..4027)
                     /locus_tag="OO439_004029"
     CDS             complement(3677..4027)
                     /locus_tag="OO439_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6318255.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4070..>4413)
                     /locus_tag="OO439_004030"
     CDS             complement(4070..>4413)
                     /locus_tag="OO439_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6318256.1"
                     /translation="RELKLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRAD
                     VAAGRRRLHIKAVCQSVREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEG
                     AQEYIRTQCIK"
BASE COUNT          949 a         1314 c         1109 g         1041 t
ORIGIN      
        1 caccgggacg cggattcata tcttccaggt cgcggcagtc attggaagag taaattcccc
       61 agttgatccc ggtggcgtag gcttcaaaac gggacttcat atccccgcgc agtaacgccc
      121 cggcgttaaa tttggcgtaa taaacgccct gcttactttt tcgtaccagt ccggtgttga
      181 tccgctgttc gatgcgggtc agatacggca ccagtgaata gttgataaat cccagcccca
      241 gctcttcgat attgttgaag gtggcgcgat cggtgttctg caccatgtgc aacggcaccc
      301 ggaacagacg acagatttct tccagctgaa acttgcgggt ttccaggaac tgactgtcct
      361 cggcgttcag cgccatcgac ttccagtcca gccccatttc cagaatcatc gggcggtgag
      421 cattgccaag ccccgtgtga cactcctcaa aatctttctt cagacgctcg taagcatccg
      481 gcgtgagctt ttgttccgta cgcaacacac cggatgtcac cgcaccatta ctaaacaacc
      541 tggcaccgtg ctcctcggtt gccgctgcca gtgaaatggc ctcacgcgca tacgcaatgg
      601 gattcagccc gacaagtcca tccagcgtca gggtgcgcac atgccagatt tcattctggg
      661 tcaacacatc cacggaacca tccggaaacg tcacctgata aaccggctgc cactggctgt
      721 tcagcttcgg ttccacacag cccggatcta tcggaagaag ctccaccact tctcccagtg
      781 cctttacctt gtaggcgtaa aaattccccc gtagacacag gcagacaata accagctccc
      841 agaattcctg cggtgtcatg tagccattag gttttgccga aaccaactta tgcagtcgtt
      901 catccaccgc ccgtgttttc agggtaccgc tgattttgta gagactgcag ggcagcatac
      961 caacagactc tgccagcacc ctgacgcaag aatagaccgc cgtcaggcgc atggcccgct
     1021 ggctactgat ccgctttccg gtataggtgt cgtatgacag cccgatagca tcagcagttc
     1081 tgctggcgtg gtcaccggca catcgctttt tcgttgaaat aatcccgaaa agaacactat
     1141 ttacctccgc cgacagactg ccgtgtacgg tcgagatatc gcgccaccag ccacgaccag
     1201 aacaggcaca gcgccccggc aacaacaaaa cccgccgggg gataaatcag ccaggcacca
     1261 tacgccagca aaagcgcacc cagcacgccc accagaggcg cgagaatcag catgatcata
     1321 attacctcag ttaaagcgag cggatcccgt aggactcaat gtggtcagac agcgtgtctt
     1381 ctttctcgta cagcatggct ctgccaaccg ccataatcag cgcaactgca ccatcgattt
     1441 tgttttccgc ctgctctttg acgggtttca ccacatcatc gttacccggc atgtttttgc
     1501 cgaccacgtt gccgatacac caggtcatga tgggattgcc gtcatgatga aaacgtcccg
     1561 attcaatcgc tgcttccagc tctttcatcg gatcggacat attggtgaag ttctggacga
     1621 tagtgacggg attcaggtct tcatcagcaa ggtcatgtga caacccggtc gccccgaagg
     1681 ggtcgatggg tgactcactg accgggctga ttttgttcgc cgctttggcc tcctcgagga
     1741 tgtagcgata atccacctcc gcaccatcgg taacggtcag aacgcccatt tccacccatt
     1801 tctgaaagcg ttcggctgtc cgtcgatctt catttttctc gacgctgtac accgtgtcat
     1861 acggtaccca gaaacgcggg gccacactgt agtaatgcgt tttaccgtca atctcgcggg
     1921 tataaagtcg cgccatgctg ttcatatcca gcttacgcgc caggtcaaag gccagaatgc
     1981 acggctgccc ctcgaactgc tcaagggtca gtgatttatc ctcgcagctc tgccagctca
     2041 ccaagttgaa atacgccgaa cgcgccgaca cccagatatt gaggtgtttt gttttaaaga
     2101 cgtttgccag acgggcgtta tttttcgcac gctgctgctg acttaacaaa aattcgcgat
     2161 aaaccgacac gccaatattt ggattggctt tttccagcac ctgcgggccg gtccagtcgt
     2221 caccttcatc aacggtatag atgatcccga acagttcatc gttaggcacc gagccgttga
     2281 gcatctcgat gacttcccgc cgcttgtcgt agcacggccc ctcaatgttg tacccggcgg
     2341 tggtgatggc ccacatcagt ggctgacgtc gcgcccccat cccggtaagc attgtggtat
     2401 aaagcgcatc ggtggcatgc tcgtgatatt catcaaccac ggcacagtgg ggtgatgaac
     2461 catcacctgg gttgccgatc agcggttcaa accgcgcgcc atcctccgga cggttcatgt
     2521 ttgaggcgtt aacctcaatc ccgaacgctt ccgtcagcat gggtgtgcgt ttacacatca
     2581 gtcgcgccgg gcgaaagact tcccacgcct gtttctctgt cgtggcaccg gaatacactt
     2641 ccgcgccaaa ctcgttatca caggcaaaac aatacagggc aacaccggca gagattgctg
     2701 atttgccgtt cttacggggg atttcggtgt acacctcccg gaagcggcgc aaccgggtgc
     2761 ctttattgac ccagccaaac gcacagcaga tcacaaatag ctgccacggc tccagcgtga
     2821 tgggcatccg tttgaatgcc cactccccct tggtgtgcgg caacagctga ataaatttcg
     2881 cggcccgttc agccaggtcc ttgtcgaagc ggtaacgaaa cgacttactt ttttccgcca
     2941 tcaggtcatc aagatggcgc tggcaggcct gaatcacaaa ctggcaggca acaatctttc
     3001 cgcgcacgac atcccgggca tactgatttg cagcatttac gttggggtaa gatttccggc
     3061 tcatgattcg ataattttca gaaacgggtt agtggctttc ttctgccccg ccaggccaat
     3121 cagacgctgg cggctgctgg ggtcgagtcc gagcattgcc cccgtgctgc tcatctcgga
     3181 ctcctgttct tttttggcgg tcagctccgg atttttgacc ctgccgccca ttgcaccggt
     3241 gatggtgttg ccctgtatgg caatattttt cacggcacgt cgccagaact cataggccac
     3301 gcaccaccgc tcaagtaccg ccaggtcagt cacgcacagc aggccctgac cgcagagttc
     3361 tttggttgtc agttgccaca tgatcgtggc gagagggaga tcttcttcag cgaaccactc
     3421 tggtggctca acacctttga tgggcgtaaa aacaggttca tctttgttca gggctcgctt
     3481 gccggggttt ccggccagcg ccttgcgcgc cgttggcttg gggcgacgcc cggaacgccc
     3541 cgccgttcca gccatatgcg gcactcctgg ttaaatttca tttttcgcgg gtataaaaaa
     3601 acgatggggc gggcagtccg gaagacgtca ggtcacaggg atttgccccg cccctcccta
     3661 caagtgagaa taattatcac cggattcgtt cgcgcgctgt tttcgcttta tggcagggcc
     3721 agcacagact ctgcaggttg ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt
     3781 ggtcgacggt tttcgcctcg ctcaccacac cgacacgcag acacaactga cacagacctt
     3841 tatcgcgctt cagaatacgg gcacgaatca ccgtccattt tgagccatag ccacgctggt
     3901 ggcggctcag tccgcgctgg tgctgcaccc agccttcacc gcgatgttta tcgcagtaac
     3961 ccgaactgtc tgtggttgta cctgcacatc cacgcttacg gcaggcacgt gggattagtg
     4021 atggcataaa tacctcatac cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc
     4081 actgagtgcg gatatactcc tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct
     4141 ctctgagggt gaaataatcc cgttcagcgg tgtctgccag tcgggggctg gttgcattat
     4201 ccacgccgga ggcggtggtg gcttcacgca ctgactgaca gactgctttg atgtgcaacc
     4261 gacgacgacc agcggcaaca tcagcgcgca gagtttcatt ttcagctttc gcatcagcta
     4321 actccttcgt gtatttagca tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag
     4381 taatggttgc gttcgccagc ttcagttctc ttg
//
LOCUS       DAJTVE010000220         4410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000220 DAJTVE010000000
VERSION     DAJTVE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..590)
                     /locus_tag="OO439_004031"
     CDS             complement(<1..590)
                     /locus_tag="OO439_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6318257.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="OO439_004032"
     CDS             complement(583..993)
                     /locus_tag="OO439_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6318258.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="OO439_004033"
     CDS             complement(999..1532)
                     /locus_tag="OO439_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6318259.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="OO439_004034"
     CDS             complement(1510..2598)
                     /locus_tag="OO439_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6318260.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="OO439_004035"
     CDS             complement(2595..3986)
                     /locus_tag="OO439_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6318261.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..4239)
                     /locus_tag="OO439_004036"
     CDS             complement(4033..4239)
                     /locus_tag="OO439_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318262.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1027 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgtttt
//
LOCUS       DAJTVE010000221         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000221 DAJTVE010000000
VERSION     DAJTVE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO439_004037"
     CDS             complement(139..3654)
                     /locus_tag="OO439_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6318263.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OO439_004038"
     CDS             complement(4106..>4404)
                     /locus_tag="OO439_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6318264.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJTVE010000222         4362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000222 DAJTVE010000000
VERSION     DAJTVE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            649..1526
                     /locus_tag="OO439_004039"
                     /pseudo
     CDS             649..1526
                     /locus_tag="OO439_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1526..1816
                     /locus_tag="OO439_004040"
     CDS             1526..1816
                     /locus_tag="OO439_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318265.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2137..2427
                     /locus_tag="OO439_004041"
     CDS             2137..2427
                     /locus_tag="OO439_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318266.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2427..2846
                     /locus_tag="OO439_004042"
     CDS             2427..2846
                     /locus_tag="OO439_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318267.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            3014..3337
                     /locus_tag="OO439_004043"
     CDS             3014..3337
                     /locus_tag="OO439_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318268.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3767..>4362
                     /locus_tag="OO439_004044"
     CDS             3767..>4362
                     /locus_tag="OO439_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318269.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1445 a          787 c          897 g         1233 t
ORIGIN      
        1 cataacaggc ttactattag gtcaccgttt ccaagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc atgaaattac acagatggtt tgctgtgctg atacattgct accgttaacc
      121 atcattaacc gtggtggact aagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt acattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat
      301 ggtcatttac aagacaggtg attcagatgt taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa tcattctgtg aaataattgt tgcaatttat aatcgcatct gttatagttt
      421 ctatatgggc tgataaaaaa gttcatgtct tgaagtggta acacttgggg aggtgtcaga
      481 tggttggcgt atattaacct ctcattcagg gtatacctca gcgttttaat catcgtactg
      541 tttcaggcat ggacttcttt atcagtccat caatccccca gatattttaa tcacacaggc
      601 taatcggttt tatccggttg gccttttcac gtatgaggta atttatgaat gtctaaatat
      661 aacccaaatc agttttataa atttgatgtg cgaatctctc aactttcaga agaaattaac
      721 gcagcagcaa gattgattta tatggtcatg gtccatagct gtgacttcta tgaagagaaa
      781 tacaaatcat attcaccaag tattgaccat ctcgcttata tggctggtgt atcaaagaga
      841 acagcaatca gagccattga gacacttgag aaacacggtt taattcaggt gcaaaagaat
      901 caggggcgtc aaagcactta tcgtgtattc acactggatg ataagccgga attacttgta
      961 agacctacat cacaggaagt tgcagcctca gtaagacaaa atgacaccac taccagtgac
     1021 aaaatgtcat gggggtagtg acaaaatgtc acccgtgtta gagtttagat aagttattag
     1081 ataataatat tacagataag aataaagaga ctggacttaa tgatgatatt aaagaagatg
     1141 actttctttc tttggatgaa ttaaatgatt ttctctctat gaatgaatct gaccttgaag
     1201 taagctctaa aaagacttct aaacaagaca gagaaatcac tgatgtgagt gttgatagtg
     1261 accataaaaa cacacagtgc cccgccactg atgatgagtt ggaccccttc gatgatgaag
     1321 aggatgaacg tgaaaaagcc attaagaggg cagagaagaa aaagagattc gagcttgcaa
     1381 gacaaaagaa caaagtatcg ggtttagacc cattcgatga cagtgaagat gattattatc
     1441 tttcaccgaa aaagaaacaa actcaggatg acaagaagca aaaaattaaa ccgaagatgt
     1501 attttgccgg tggaggaatt aagtaatgaa agaatatcac ggtgaaaaga gatacaaaga
     1561 ttacctgtta aagcgttaca gcatttctcg tgaaggtcat cttatgaagg atactcacgg
     1621 ggaagtttac cggattagac caaagaaaga agggaggggt tatttctttt acgatggtgt
     1681 gacagattta aagattgacg ctttgaggtt tgcagtgctg tatcattttg atatctggaa
     1741 ttcaatccac cagctacgat taaaagacgg tgaccccagc aacttaaagg ccaccaatat
     1801 tatcacaaaa cgttagtttg ttttaccatc atcaatcgta attgtgattt catatttgaa
     1861 aagcttaatg ctgaaattga atcgtgtact tctggcgata atcattttta aaagctgata
     1921 cacaagaagc accagaaaca caaagcaacc taagcttgcg aatctgtgag attcagcaat
     1981 tgtttcaaga aagcccatta attcccctta tttatcaaag ggaatgacct gtatcagccc
     2041 cctacaacca tattaacatg taaatgagtt gttgacaata tgttataaat atgttaaagt
     2101 tagttataag tcaaagattt taagaaggaa aatttaatga gacacacagt tacacgcctt
     2161 ttgccagagg catattccag actggcagca atcaaagaca aatacccagc catgagccac
     2221 acagcacttg ttggtgagat tttgaaggaa cacatagccc ttacaccgtt tgacgctatc
     2281 aagggcgttg cagagggttc taaggccatc agaatcacaa cgggccaact ggaactactc
     2341 aaagcacagt ccgcagtggc aggttgctca atgattcagg tgttgagtgc caaggttcat
     2401 caactggcgg ataagctggg ggtttaatgc tatgcacatt tgattatcat gcgttttatc
     2461 gccatagagg tcaccttgtc cgtgagaata acggaacatg gaaagattta actcgtaatg
     2521 ctgaaccctt cacacaccag caattaaaat cccgcttctc cagatggaag aatgcaccag
     2581 cagatactgg tgattttgag attatccata ccttcgatta tgcctcagaa gatgaccttc
     2641 acgatgcaat taagctgatt gaagacttca atacctcaga agatgtggat ggtgacaaat
     2701 ataaatcccg tggtaaccgc tataaccgtg atgatatcac tcaggccata aatgctggtc
     2761 aatctcagcg atacattaca gaaaaatatg gtatttcacg gagacagtat ttctacatta
     2821 agccattgat taataaagac aattaaaaat aaccatctaa gaaacggtag aatgcacttt
     2881 acaggcagag ttgcacctta atttgataaa ttaccttacc agccagtgca actccctctg
     2941 taacccgcat ggttactggc ctcagaatgg ctattttaag aggtctgagg tgtgtttcat
     3001 aggagattta aaaatgaata attacccaat gcaaattatg gttgataacg acactgctat
     3061 gatggtgcaa agcttcattg atgcaggtat cgcaattaat tttgataaat tactaaagct
     3121 gatggctgat aatgtagaaa acatctctga ctttattcag tcggtggagt ttaacgagcc
     3181 tcgaatgatg ctaccaatta cagacagcaa catgaaaaga ttggttatcg agcaaacgaa
     3241 catatacagt atttccccag agcaattttt gaaaggtgct gtgacaatcc tgtacagtga
     3301 taatattctg gtgacggatt ctgtgagggt tcactaatgg gtttaataat tgcttggacg
     3361 ttgcttggct tatttgttgg ttgtattatt aacaatgcgg taaataagaa ttgatgttat
     3421 caacctgtta aaaagctgtt gacaaataac cttaagcatg gcataatttt aattatggga
     3481 gagcaattct ctcttatttt tctttcaata atccgatgga tggatattct gagttggaaa
     3541 ggtgcaatgc ttttcgtcat ggcctttcag gttagtctga gaggctatct caaaaagtac
     3601 tcttgttcat tcattattga atcctttgcc cttcacagaa atgtttaggg ctttttcagt
     3661 ttttaaaagc acacttggca ggttcttgtt cagtcagact cttcacctct gattgctgag
     3721 tgtgcttcta taaattgaaa taacaaataa actaaggggt gaacgaatga ttagaaatga
     3781 acatgaaatg cttatcgaac acagaccaag tgcacttgat attcacgaat taactttgcg
     3841 tgaatctttt gatagagctt ggcgggaatc acacgaagta aataaaggtc catatattcc
     3901 accagaacca ctcgagattc caagagttga aattgatttt tctatgaacg aacggtgtga
     3961 acttgatttg aagttgaagc tacaacgccg atatttccaa gctattaaag actctcaaga
     4021 agcaatgaga agtgtttacg agaaaattca gtccaagctt gaagacgaaa ttgacgaaat
     4081 ggttatttcg atggagcttc aaaccaattc ttatgcttat ttctctcgac tagaccgtga
     4141 cggttggggg tatgaccctg tagagattgg taaagtgatt gcagatttac cagaaggtca
     4201 aagagttgtt cgtacagaaa gagctgtcag agcaccacat cgactgtctc taattgttgc
     4261 tgataaatca caagctgagc ttgaagaaat tgccaaagag aaactgatga aagcaagaga
     4321 aaatgagatt aatttgctga aatcaagact ggctaagctg at
//
LOCUS       DAJTVE010000223         4327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000223 DAJTVE010000000
VERSION     DAJTVE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(289..363)
                     /locus_tag="OO439_004045"
     tRNA            complement(289..363)
                     /locus_tag="OO439_004045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Gly,seq:tcc)
     gene            complement(367..442)
                     /locus_tag="OO439_004046"
     tRNA            complement(367..442)
                     /locus_tag="OO439_004046"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Thr,seq:tgt)
     gene            complement(445..521)
                     /locus_tag="OO439_004047"
     tRNA            complement(445..521)
                     /locus_tag="OO439_004047"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Arg,seq:tct)
     gene            complement(523..599)
                     /locus_tag="OO439_004048"
     tRNA            complement(523..599)
                     /locus_tag="OO439_004048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Ile,seq:cat)
     gene            complement(789..1352)
                     /locus_tag="OO439_004049"
     CDS             complement(789..1352)
                     /locus_tag="OO439_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6318270.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1456..1674)
                     /locus_tag="OO439_004050"
     CDS             complement(1456..1674)
                     /locus_tag="OO439_004050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318271.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1833..2219)
                     /locus_tag="OO439_004051"
     CDS             complement(1833..2219)
                     /locus_tag="OO439_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6318272.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQN
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(2209..2580)
                     /locus_tag="OO439_004052"
     CDS             complement(2209..2580)
                     /locus_tag="OO439_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6318273.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(2580..2837)
                     /locus_tag="OO439_004053"
     CDS             complement(2580..2837)
                     /locus_tag="OO439_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318274.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2834..4225)
                     /locus_tag="OO439_004054"
     CDS             complement(2834..4225)
                     /locus_tag="OO439_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6318275.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(4222..>4327)
                     /locus_tag="OO439_004055"
     CDS             complement(4222..>4327)
                     /locus_tag="OO439_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6318276.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         1048 a         1192 c          979 g         1108 t
ORIGIN      
        1 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      181 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      241 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctcgctg gagcgggcag
      301 cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc
      361 cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct
      421 ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct cgcaccgtca
      481 acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc cttgctggac
      541 ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc taaagggccg
      601 gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgacca ccagcacgtt
      661 taacgtcctg tgccgttttt caggtataaa aaaacccgct catcggcggg tttaagctgt
      721 gtggcgtagt aaccactctt aacaggatat tcaacttttt acgatcgtaa agttttcgga
      781 aaaaattttt aaaaccgcat caaccgctcc accagttgct ctttacgggc aacgatccac
      841 ccgtgttgct ccagataaaa tttaaaccgt tccagagtac ataccatcgc atcggcgggt
      901 accttttccg tgaactcaac ctgaccgtgt ttatcgaagt ggctcagtaa tgcgcatcca
      961 tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt tctggttgaa ataacagtct
     1021 tccagttttt cgaacacttc ccacgcctga tcggtttcga gcattttggc gtgacgggct
     1081 gcgccacgtt ctgtccagag gatgagggag cgaacgttac gggcaatttt cacagaaact
     1141 cttaaagagt tttcgttctt caactctcgt aacgcatcac cttctatcag gaaaaaatgt
     1201 ttaccttcta taaaccgttc tttgtttcga ttgaaattgt ttgtcaaacg ctggcgctct
     1261 gttccgtaca aatcagccag cagctctgtt gtcatgacag gaagatggtt atgcgtaagt
     1321 gacgggagtt tttcaataga cgtgcgattc atagacatgt cctttcgaat attttgattt
     1381 accccttttg agagggtgac cgggcgctca aaaccgtcga aagtcggcgg gcgtatttcc
     1441 attgctggta ttgtattagc cgcacacccg gtcataaacc aagaattctg gacacaaaaa
     1501 aaccacatgg ctatcgggtg tggataccgc tttcgaggtg ttttgagcac cgcggggaaa
     1561 tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc caatcatcgg gtggtgagac
     1621 gcacagggtt ggaactaccg ggaaaccgac cggcgagctt ttcagctccc ccatgcgccc
     1681 caccataatt cagatgtgcg cgtgcatacg acaataaaaa acacgctcgc ggcgtgtgtc
     1741 tgtcgcggtc tctatccggg gttccaatcc cgacgccaga ttttgctggc gcgtgaggaa
     1801 tatagccccg gacaatgtgt ctggtcaagc acctacataa ttcgttctac gtatctgtcc
     1861 atctccagcc ggatatcaag catcatcaac atgccatcaa taaccccttc cgcgttctgc
     1921 aggcgcttgc caatacaggt atccgaacac ccatgtttcc gtgccagact cataaaagtc
     1981 attccaccta cgtaataatc caccagcaaa tcgtgcaaat cctgattttt cttgttcaac
     2041 cgggccatac agccacaaat tatcattgca tcatcatcag aacactgagg gcgtgatttc
     2101 actttcgacg gaattaaccc tttaaaacca gcagcgatcg acgcccagga tacatcttca
     2161 tgagtgtttg cagcccaagc cccccaccgt tccataacct gctgaatatc acgcgccatc
     2221 gttatcacct gtgatttcat aaatcttcac gcccagtcgc ccaccaggaa cgagctgacc
     2281 gcgcacaata ttgatttcat caaactgctc gtcgtctatg agtagtccgg catgcgtcag
     2341 cgcatccagt ggtgctttca ggatattgtc caggtcacga cggcgcttat ccggtggctc
     2401 tgcaataatc tttatcgcca gccttccgga caggtttaat ttcagccgct gctggcgaac
     2461 aattagcgtc acatcacggc gataacgctc accaactttt gatacaaaat atgtgctgcc
     2521 acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt aaaataaact catggcgcat
     2581 cagcgcagca cctcctgcac cattttttca aactttccaa ctttggtttc cagctctgcc
     2641 acacaatcaa cgagctcatc caccgctttt tgtgcgcgat gtttcgcctg catcagttcc
     2701 ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg ttacacctgt tttttccagt
     2761 tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca attgttcaga ggtaaaagtc
     2821 attgtctggt tgttcaaaag aaacgctcca tcttactgct gtcggtacgt gtattgctgt
     2881 atctgcgcgg ctggggctgc cgcattgggg tggaaagaac ctgtgcgctt tcctggtcca
     2941 caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt ccccagagaa ccgttacgtt
     3001 gtttcgtgat attaatttct gctatgcctg tagcctgtgt atccgggttg tacacttcat
     3061 ccctgtaaag catcagaatg atgtctgcat ccgcctctat ttctccggaa tttttcaggt
     3121 ctgagttcat gggacgttta ttgggtctgg actccacacc gcgggagagc tggctcagcg
     3181 caatcaacgg aaaaccaccg gattttgcca ggcctttaag cccctttgag atttcaccca
     3241 cggcaaggtc atgacgcccc gtggttcggg tttttatcag cccgagataa tcaaccacca
     3301 ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt tgttgcgcat atctcatcaa
     3361 tggtcaggtt cgcctggtcc accatccaga tattgcgccc ggtcatccgc cccacccctt
     3421 gtgagaaacg cgcccagtct tcatcttcaa agtgagccac agatttcagg cgtgatactg
     3481 gcatccctcc agccgcagac accatacgtt caccaatctg gatgttcgcc atctccatgg
     3541 tgaacagaag cacaccatgc ccctgctcag tcaccttgtc gatgatgtcc agcgcaagtt
     3601 cggttttccc catcgaagga cgggcggcaa tgaataccag gtctccgggc tccataccgc
     3661 ctgtttttgc gtccagttca tcaataccgg tcatcaacgt cctggatttc tccagcccct
     3721 gattccggca ttcaacacgc tcaaccactt ccggaagcac atcatcaata tgtaccggct
     3781 gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt ctgcgcatcc ttcagggcat
     3841 cttcagctgc ttcacaggta tgcgcatcac gtaatttctg cagcgcctca ttcagtgttt
     3901 tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc aacatagtgc tccagtgaag
     3961 acttcaccca ggttttacgc ccggtatcag taatcaccgg ggcaagttcc ggcatctcat
     4021 tacacaacag cacggggtca atcactcctg aaacacgggc ctgtctgcag atgcctgtgt
     4081 agatatcccg atacgctcgt acagaaaaaa tgtccgccgg tagtgtgatc agaatatcca
     4141 tcacttcatg atctgcccca cgcagaaaga atgcgccaat gacagcgcct tccaggtcat
     4201 cattacgcca gactggtgtt gtcatgcagc cacacctctg atacaagaac ggtagctggg
     4261 ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc ctgtcggcaa tgcgggggct
     4321 gatgaac
//
LOCUS       DAJTVE010000224         4318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000224 DAJTVE010000000
VERSION     DAJTVE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..581)
                     /locus_tag="OO439_004056"
     CDS             complement(<1..581)
                     /locus_tag="OO439_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6318277.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYTSYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(644..1093)
                     /locus_tag="OO439_004057"
     CDS             complement(644..1093)
                     /locus_tag="OO439_004057"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6318278.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="OO439_004058"
                     /pseudo
     CDS             complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="OO439_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1086 c         1164 g         1041 t
ORIGIN      
        1 aagttcggga tcatcgcgtc aacggtgatg gcgtcagcag gctgtacgcc gccaaccagc
       61 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
      121 gaggtaattg gccccaacat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
      181 agcaccatac tgataatggt ggtggcaacc ggcgtcataa tatattcgta tccgaggaag
      241 gcatacgaag tataagcaat aacgcgcggc aacagtgagg tgaaaaagaa caggtttacg
      301 aaccagtagg tataggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccat
      361 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
      421 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaaga
      481 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
      541 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatga
      601 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
      661 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
      721 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctca acggtttctc
      781 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
      841 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
      901 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc tgtctccacg cggtaagtca
      961 atgagtcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
     1021 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
     1081 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
     1141 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
     1201 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
     1261 gttggagccg aggccaagca gctggtgatc gaggttcagg gtgatgtcat cactgcgctg
     1321 cagctcgtta cagtgctggg cagcgtggat gttttcctag gtatagtgcc aggcgctgaa
     1381 gttaattggg cgctgcggaa ccaccagcag accgttaccg tggcggttag tcagtgccgt
     1441 ccagcggaca tgctgacggt taccgttgtt ctgcgggaag ggatagttct cgaacatggc
     1501 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
     1561 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaattc ccatggtgaa
     1621 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
     1681 aacctggcca tcggcagcga tgcgccagat gtaggtgcag cgcattccga aatcaaacac
     1741 cggcggggca ataaccgtgc ggctgatgat caacacttca ccctcgctct gttctacggc
     1801 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
     1861 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
     1921 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
     1981 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
     2041 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
     2101 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
     2161 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
     2221 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
     2281 cacctctgcg tgcagggggt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
     2341 caactcgccg cgagtcagat cccgcgcgtg gatttttacc ggcgcgataa cctgtttgta
     2401 ctctttcagg cccggtcccg gcgtctgatc ggaatagatc aaaccatcaa gacagaagtt
     2461 atagttgttg ggatagtcgc cgtagtcgcc gccgaattta taccagacat tgccgtggtc
     2521 gtcctgtgcc tggatcccgt ggtcgcacca ctcccagaca taatgaccct gaatgcaatc
     2581 gtgcttatag aagacgttct ggtactccgt cagcccgccc ggtccgttcc ccatcgcatg
     2641 agcatattca cagatgatgc gcggcttcgg atgcgggtat tcaccaaact cattcatcag
     2701 cggcacgcgg gtgtacatgg tggaaataat atcgaccact tcagcatcgc gatcttcttc
     2761 gtaatgcacc agtcgcgtgt catccagcgc cttcgctgcg tggtacatcg cgcggatgtt
     2821 acagccatag ccggattcat tgcccagcga ccagatgatg atcgacggat ggtttttctg
     2881 cgcgtgaata tggcgaacaa tgcgctcgac gtagactttt tcccactgcg gatcgtcggt
     2941 aatacggctg atatcgccga cattagcaaa gccgtgcgat tcgacgtcgg tttccgccat
     3001 cacaaacagg ccgtagatat cacacagttc gtaaaaacgc ggatcgttcg ggtagtgagc
     3061 ggtacgcacg gagttgatgt tgtgctgctt catcaactgg agatctttct cgacgcgatc
     3121 cattccaacg gcgcggcctt tgcgatgatc gttgtcgtga cggttgacgc cgtgcagcat
     3181 cacataacgg ttattgatcc agaacagacc gtcgcgcact ttgatatcac ggaagccaac
     3241 gcgttgtggt accacttcca gaacgttgcc gttggcgtct ttcagcgtca tgaccagatg
     3301 gtaaagataa ggggattctg ctgaccattg ctgcggctgt tcgacagtaa aagcaaagct
     3361 ggcgctggtc agtttttcaa ttgccaaatg atcaatggcg ctgctgtgca ccacgcgttc
     3421 gccatcaaac agggtatatt ccagcgtcgt gacgacaggg gaggcggcga gattttccag
     3481 caccacttcg caggaaagcg tggcatcgca ataggcttcg tcaaagtcgg tacgcacggt
     3541 gaaatcgtta atatgtgtta gttgttttcc gatcagataa acatcgcgga agatccccgc
     3601 tgaccaccac atatcctggt cttccacgta ggtagagtcc gcccattgca tcacgcgcac
     3661 acacaacagg ttgtcgccgg ttttaaccat cgcgctgatg tcaaactctg cggtcaggcg
     3721 actgcccttg ctgaaaccca catactgacc gttaacgtag acttcaaaat aggtttcgac
     3781 gccgtcaaat ttaatcagcg tctgtttacc ctgccagccg tcgctgaggg tgaaaatacg
     3841 ttgataggca ccggttgggt tatcgctggg aacaaacggc acatcgatgg ggaacggaaa
     3901 accttcgtcg gtatattgca gtttgccgtg accttccatt tgccacatgg cggggacggt
     3961 aatatgcccc cagtcagcca ttaactcaga ggtgaaggct tcaggcactt gcagcgggtg
     4021 gtcaaaaaag tggaaattcc actgaccgct taagggcaga aacaggctgc tggtttcgcg
     4081 ggcaaaggta cgcgcttgcg caacagaatc atatgaaaaa aagtacgcac gtggcgcaag
     4141 tcggttttcg tgggtgagct gaatgttttc ccagcgattc atagggcctc ccggtagaga
     4201 gaaatcggaa ccattcatgg tgatggtagt ttgtgtaatt gctggggagt ttagtaaaat
     4261 ttttactaaa aagtgacagg taaatgcatt tactttaact ggatcacgaa aataaacg
//
LOCUS       DAJTVE010000225         4248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000225 DAJTVE010000000
VERSION     DAJTVE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="OO439_004059"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="OO439_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6318279.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="OO439_004060"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="OO439_004060"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6318280.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="OO439_004061"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="OO439_004061"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6318281.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4087..>4248
                     /locus_tag="OO439_004062"
     CDS             4087..>4248
                     /locus_tag="OO439_004062"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6318282.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT         1048 a         1079 c          981 g         1140 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgtg atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgctcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcaggt
     3841 catttcgtaa ttacattaat taaaaaactt aaaaccatta aataaataaa aaaccactaa
     3901 ctctatgtga aataaatcaa aatttcacgc tgcaatactc cttagaatgt atagcgaaag
     3961 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcaa
     4021 cttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4081 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4141 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
     4201 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaatt
//
LOCUS       DAJTVE010000226         4243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000226 DAJTVE010000000
VERSION     DAJTVE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..1406)
                     /locus_tag="OO439_004063"
                     /pseudo
     CDS             complement(42..1406)
                     /locus_tag="OO439_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1471..1590)
                     /locus_tag="OO439_004064"
     CDS             complement(1471..1590)
                     /locus_tag="OO439_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6318283.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1745..1963)
                     /locus_tag="OO439_004065"
     CDS             complement(1745..1963)
                     /locus_tag="OO439_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318284.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2317..3795)
                     /locus_tag="OO439_004066"
     CDS             complement(2317..3795)
                     /locus_tag="OO439_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6318285.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1195 a          926 c          754 g         1368 t
ORIGIN      
        1 ataagtgata ctggttgtct ggagattcag ggggccagtc tagcaactaa agcactgccc
       61 gtctcctggt taaccccatg tgctataccg ccggtataga caatcagcga gcctccggcg
      121 ctaatattgc tgccagttgc cgtaccatct ttctcaacaa cctgccggct cccggtggaa
      181 atatttgtcg tgtcagcttt cccgccgctc ttgatatttt gtgttccgct gttgatattg
      241 gtacccgtgg caataccatg attattaata ttctgtgtac caccattgat tatggtattg
      301 gtcgcgtttc tggtaacatc cattaccccg ccgttatcta ttcgggtcgc atcagcttta
      361 gcattcgtta aaactgacat tgttccttta tctttaataa tcgtcttatt tgccgaacca
      421 tatgcgttta tgtctaaatg accaccgttt tccagcaaca cattgtctgc cacgtgattg
      481 tggatggaga atgcaccttc actattcgta ccgctcaccg tcgtaccgtt agtgtttgtt
      541 tttaaaattg caccatcgtg ctgggtaaca tttgttgccg taccaccact aacatcaagc
      601 acgccaccgg aataaacttc aataacatcc gaggagctgg tgtaatcaac aatttgcgta
      661 ccaccagaat agatctgagt attttttgcc gttgacttat tattcagaga ctgagttcca
      721 ccttcaatcg tcgtgtccag cgcatggctt tcatatactc tttgctcacc gccattttta
      781 atggtggttg ttcctactgt gctctgttca acatactgtc gtccaccatt tatgattgtg
      841 ttcgttgcca tacttccttg cgtgatgtcc tgataaccgg acttatttat cgttgtacca
      901 tcaacatgcc cctgagttgt tactgtctgc cctccaccat caatgatggt tccattcgca
      961 gtccccccac ttatgtgtga attaccaccc ttaattgtcg ttccattgct gataccgcct
     1021 gtataaacgt cctgattgcc actgtcgatt atcgtaccgg tggaaatgcc cccgtcatga
     1081 attgtctgtc ttcctccgtt aatggttgta ttattagact gccccatata attgttattc
     1141 cttccaacat cttgatatcc accagattca ataagacttc cattagatac tccgccataa
     1201 acactctgct ggccatggtt gataatatga gtgttatttg ttgtacctcg ttcatctact
     1261 cgttggttgc catctacagt ctcatcgttt accacaccaa taacattagt agtaaaggcc
     1321 gccatcacgg gcggggcata tatcaaggca gatatcaata aggaaagtac tgagcggcga
     1381 caataatggg gactggtcct gttcataaat ttcatcctct gaaaagtgaa tactgagtag
     1441 cgtttaagcg actttagctc tgctgcaaca tcagcccgca ggcaccagac cggggggttc
     1501 atcctgaaga gacagcgcaa gtgtattgtg ttcaccgctc atcaaagaca tcatgatgaa
     1561 atgatgatgt tccacataag aaagaggcat tttttaaaca cagtgcgctg aagtgtgatt
     1621 ggataaaaaa gtcaatctat tcaggaaata cgggcgtatt cttttctttc gacaatcagg
     1681 ccgtcggcaa aataaaatga tttacataat cgtttctgat gaatatcttc tgctcacata
     1741 aaaatcacac aataactttg agatcgcaga ttcttttact tttacagcat tcgtcccccc
     1801 attgttgggc aaatatagat tgggccagag cacgaaagtt aataccacgt ttgcacagct
     1861 cctccaacag cacgacaaga tgccacatac tgcgccccag tcggtgcggt ttacagacta
     1921 cccgtgcgtc caccgccgat aatgtcctga gcagtttttt cagtccggac ctcctgccac
     1981 ggttttacgt tgaagaggta accctgagca cgcagttctt cagtcaggcg tggtgcaccg
     2041 taacgctgtt attgctgggt aagatcaaaa aactttcagg cagctaagga aagttgaacc
     2101 agacattaga tgaaatattt caaccaatta cagcaccaat ttaaactcag taaatcacag
     2161 cacagatcaa ggggttacgt gaaagcgtag cccctttttc tttggtagtg gcagcaaaat
     2221 ggttgtagtg taaaaaataa tcccgtttaa tcaatcaata atacatattg tttcaatcta
     2281 cgttattatc tctttgtaaa aatagccatt tattaatcat tgaaaactgc ttttagaact
     2341 tgatacaacg ggactagtca caacaggact attctcaacg ggatcatcct cagaggaact
     2401 atcatcaaag tcatcatcca taaataaaat atcatcgaat ggtgccacgc ccgtgatgag
     2461 ttttatttta ttattacgat cagtcaagac tccacttaaa ccgttttcgc tcacaggttt
     2521 taatgatttt tcattactct tgttgtaagc aggcgcatta aaaatacacg gagtatcaag
     2581 atcaaacaat gacgttctcc agttcacata ttgaatatca tagttactga agttctgtcc
     2641 agaaaagaag catcccttaa aatccaatcc acctaaatta tataaaccat cctcttcttt
     2701 ttggagagta atgttaattc tggctatctc ccggacaaaa cttccttttt tgtatttgaa
     2761 taccgtttca agattttcac cagacagctt gacttctggg aataatttaa aattaaaccc
     2821 tacatcatta ttattcaaag tagatgaacg aaagatggaa aaagcttgta gtgctgaatt
     2881 atagctttcg attttatctt gaggctgcgc tcttggtaaa aacttatagc agcactcata
     2941 aaaattgaat aaacgttctg aacaattccc atttttatca attaataccc cccggaattc
     3001 tttcacaaag gcatccgtta ttttttgaat atctatggtg tgttcctttg actctgacaa
     3061 agatggagtc tgttgaagtg cattgctcat ctggttaagg aacatctgca tttctttaaa
     3121 tttatctcca tagtggcctt taacttgctg agagttttta gttacaggtg aaaccttatc
     3181 tatttggttg tcatataaat tatgtggttc catacagaca tttggttgtg tttctaaaat
     3241 aacactacgt gtactacctg gcttaaatga atcaacatga aaatcacttt ttcttatatt
     3301 atcgaatagg gtctgttcat ttctcttagc gcattcaata aactgatcaa cattattttt
     3361 attcgatgac aggtcagttt cagaaaccgc attttctttg ttctccagct ttagtttaat
     3421 gagaagatct tcccagacct gcttgcttaa acatactacg tcaggctcac catcagtaac
     3481 taactcttca ttattatcaa agtatacgtt gaatactttt aagttatttt catcaacatc
     3541 atatttaata cgttttaatt gctctccaga tcccataatg acaaaggcgt tgccttcatg
     3601 atatatacat tcagacatca ttttttgtaa agtttcaaga gcaccgcgat acgtccctga
     3661 ggcagcttcc ttacaaatta aatttataat actataagct agtagctcca tggattcaga
     3721 agattttctt tcattacaat ttccactatc tataaatgag ctagtgttaa attcgttgct
     3781 tttactaact aacattgtct attcctcagt taatgtctac atggctattt ttaattttat
     3841 tattgtttgt cactataaaa aatcgctcat ttgagacaat tgctggcatt aacagcttca
     3901 tatgctatac atggtacttt taattaaatt agcacaagaa tgttaaattt aataaacaaa
     3961 aagttatttc gctgtatgat aaaaaccacc cgttataatt tattagtgaa attcgttttt
     4021 cgagtgttag aaatttatat ctaaatagcg ttgattaatg agcaaagcca gactcctgta
     4081 acgctcacaa gactcatcta cctgcgacgg gtattctggt cagtagtaga tgtttaaggc
     4141 gtggcagaga catttcatcc ttactctacg gcattggccc gcatacaaat cgtggttgtg
     4201 acactcactt atcatcagtg agcgaacaga gaatagttca gtg
//
LOCUS       DAJTVE010000227         4193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000227 DAJTVE010000000
VERSION     DAJTVE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO439_004067"
     CDS             complement(<1..201)
                     /locus_tag="OO439_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318286.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALP"
     gene            430..1038
                     /locus_tag="OO439_004068"
     CDS             430..1038
                     /locus_tag="OO439_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6318287.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1136..2527
                     /gene="selA"
                     /locus_tag="OO439_004069"
     CDS             1136..2527
                     /gene="selA"
                     /locus_tag="OO439_004069"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6318288.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2524..>4193
                     /gene="selB"
                     /locus_tag="OO439_004070"
     CDS             2524..>4193
                     /gene="selB"
                     /locus_tag="OO439_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6318289.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANM"
BASE COUNT          933 a         1084 c         1243 g          933 t
ORIGIN      
        1 gggcagggcg atgtcggttt caccgggaag gacctttgca ccgagcaggg gattgaccgg
       61 atggccggac gtcactccgc cggggcacac cgaacaggcc acaccggtgg gggcaccgat
      121 gcgcaccccg gctgaaccct gaacaatgct accgccatac tgcgtcatgt cgccctgacg
      181 cgctgccggt tttccgctca tctgctcatc cttgttttat cgggttattt gtctgcttcc
      241 aggcaaaacc ctacagattt ataagaaagc cttaagtgag atatccatca gatagaaaca
      301 attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag taacaaacca
      361 tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc ctcgcacaaa
      421 ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa actttctatt
      481 ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta taacgcggac
      541 aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac cgaagagggc
      601 gaatgctggt ttgattcgcc gatcatcgct gaatatattg agttaatgaa tgtcgctccg
      661 gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga ggcgctggcg
      721 gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc ggcggcgcag
      781 cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct ggatgtgctg
      841 gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc gactatcgcc
      901 attgcctgtg ctgtcggata tctcaatttc cgccgcgttg cgccaggttg gtgtgtcgac
      961 cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc
     1021 gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt tacaatccgt
     1081 ggttatgtta aacgcccttc tccgtgtgag agggccttga tcagccaggt ttcctatgac
     1141 aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat tgcgcgatag
     1201 ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat tgttgcgtca
     1261 gatgctcgac gaagcgcgag aagtgattcg tgacagccag acgcttcctg cgtggtgtga
     1321 aaactgggcg caagaagtcg atgcccggtt gacgaaagag gcgcagagcg cgctgcgtcc
     1381 ggtgatcaac ctgacgggaa ccgtgctgca taccaacctt ggacgagctt tacaggcgga
     1441 agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg agtacgatct
     1501 ggacgacgcc ggacgcggac atcgcgatcg ggcgctggcg caactgctgt gccgtattac
     1561 gggggcggaa gatgcctgta tcgtcaataa caatgcggcg gcggtgttat tgatgttggc
     1621 ggccactgcc agcgggaaag aggtggtagt atctcgcggc gaattggtgg agattggcgg
     1681 cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg aagtagggac
     1741 caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata ccgcactgtt
     1801 gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga tagatgaagc
     1861 ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt taggcagtgg
     1921 ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc aggagttgat
     1981 tgcggcgggc gtcagtctgg tgagcttctc cggcgacaag ttgttaggcg ggccgcaggc
     2041 agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc cgctgaagcg
     2101 tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc gtctttattt
     2161 acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga
     2221 ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt acggcgcgga
     2281 gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc tgccggttga
     2341 tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta gccaccttga
     2401 gtcattagcc gcccgctggc gtgaattgcc agtgccgatg attggtcgta tttatgacgg
     2461 acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg agatgttgtt
     2521 gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt attgcaggtg
     2581 attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat gaccatcgat
     2641 ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctagttttat cgacgtaccc
     2701 ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga tcacgcgctg
     2761 ttggtggtgg cgtgcgatga cggcgtgatg gcgcagaccc gtgagcatct ggcgattttg
     2821 cagctgaccg gtaacccgat gctgacagtg gcgctgacca aagccgatcg cgtggacgaa
     2881 gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata cggttttgct
     2941 gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag
     3001 catctgcttc agttaccagg acgtgaacac gccagccaac atagtgtccg cctcgcgatc
     3061 gaccgcgcat ttaccgtaaa aggtgccggt ctggtcgtca ccggtacggc gttaagcggg
     3121 gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat gcgtgtacgt
     3181 gcgctgcatg cacaaaacca gccaacagaa accgcccatg ccgggcagcg tatcgcgctt
     3241 aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct gcttgccgat
     3301 gcgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac gccgctgacc
     3361 cagtggcagc cgctgcatat tcaccacgcc gccagccacg ttacgggacg cgtttcgctg
     3421 ctggaagata accttgccga gctggtgttc gacacgccgc tgtggctggc ggataacgat
     3481 cgcttagtgc tgcgcgatat ctcggcccgc aacacgctgg cgggggcgcg tgtggtgatg
     3541 cttaacccgc cgcgtcgcgg taaacgtaag ccggaatatc tgcaatggct ggcgtctctt
     3601 gcacgggcgc agagcgatgc cgatgcgtta tctgttcatc tggaacgcgg cgcggttaac
     3661 cttgcggatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga attgctgcaa
     3721 cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc cgcccgctgg
     3781 cagcggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga tgaacctggt
     3841 cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc gctggtactg
     3901 ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg ttggctgcat
     3961 ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca aaaagcagag
     4021 ccgctgtttg gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac gggtaccgac
     4081 gagcaggcaa tgcgcctgac gctacgccag gcggcgcagc aaggaataat taccgcgatc
     4141 gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat gat
//
LOCUS       DAJTVE010000228         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000228 DAJTVE010000000
VERSION     DAJTVE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO439_004071"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO439_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6318290.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO439_004072"
     tRNA            complement(330..405)
                     /locus_tag="OO439_004072"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO439_004073"
     tRNA            complement(412..486)
                     /locus_tag="OO439_004073"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO439_004074"
     tRNA            complement(603..687)
                     /locus_tag="OO439_004074"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO439_004075"
     tRNA            complement(696..771)
                     /locus_tag="OO439_004075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO439_004076"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO439_004076"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6318291.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO439_004077"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO439_004077"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6318292.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO439_004078"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO439_004078"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6318293.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTVE010000229         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000229 DAJTVE010000000
VERSION     DAJTVE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1058)
                     /locus_tag="OO439_004079"
     CDS             complement(<1..1058)
                     /locus_tag="OO439_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6318294.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSE"
     gene            complement(1055..2452)
                     /locus_tag="OO439_004080"
     CDS             complement(1055..2452)
                     /locus_tag="OO439_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6318295.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2607..3605
                     /locus_tag="OO439_004081"
     CDS             2607..3605
                     /locus_tag="OO439_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6318296.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3716..>4168)
                     /gene="ompG"
                     /locus_tag="OO439_004082"
     CDS             complement(3716..>4168)
                     /gene="ompG"
                     /locus_tag="OO439_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6318297.1"
                     /translation="WLSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLE
                     RGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHD
                     FNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1013 a         1137 c         1032 g          986 t
ORIGIN      
        1 ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat aagctgacga
       61 cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg gcaaagctcc
      121 atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg agctgcgcgg
      181 gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc gcgcaccagt
      241 tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg caaacggctg
      301 taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg cagattgcgc
      361 caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat caacgttgat
      421 tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg ctggaccaaa
      481 tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga ggcataccag
      541 cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt ttcgcaaaat
      601 gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc gcgttcatgg
      661 gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc tacgccagcg
      721 ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg ggtttgccag
      781 gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag agccagcccg
      841 cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc cattaccgct
      901 tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa attttgcgcc
      961 tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac ctccagcgga
     1021 ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct cctattaaaa
     1081 attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg
     1141 ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg
     1201 caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga ttaccgcgca
     1261 atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc tgcaccgagg
     1321 ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag gcatcctgaa
     1381 tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca tggtccgctt
     1441 tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga aagagcgtgc
     1501 gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata tcattaaatg
     1561 cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg cggtcaaagc
     1621 gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa ttaaaccgct
     1681 cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac tcgccccagg
     1741 tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata tctcctggca
     1801 agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg tggagataat
     1861 cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct agcgggtcca
     1921 gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca ttgagtaagc
     1981 ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg tcgagcaacc
     2041 attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg aagtggcgta
     2101 gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg ccgcgcgtag
     2161 gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca taggtgaagc
     2221 gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc tcttcacgca
     2281 ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga ttgaccatcg
     2341 cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg cgcagatgtc
     2401 tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc attgctgtcc
     2461 tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg ggggccaggc
     2521 cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga tgtgcagcat
     2581 tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt gccagggttg
     2641 caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat atctccccgg
     2701 aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca aacaagctag
     2761 cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc cgttcgacca
     2821 aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc gccaaagctg
     2881 aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa gacttacaaa
     2941 aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta agttcgccag
     3001 cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg gtgattggca
     3061 aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt ggcgacagca
     3121 ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc ctgcatgctc
     3181 cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc ctggctgcgc
     3241 ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat gaaacagcac
     3301 tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg tttgccactg
     3361 acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt gctattccgc
     3421 agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta acgcctgcac
     3481 cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag ttattattcc
     3541 gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat atgacgctga
     3601 aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc ttatccggcc
     3661 tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc cattatcaga
     3721 acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct tcgtcgtgat
     3781 cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa tcataaccgt
     3841 aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc tgccagtccc
     3901 agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg
     3961 tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg cggctgtcgt
     4021 ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt tcgttgaagg
     4081 tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta gtgttcagat
     4141 cgttggcaaa tttatacatc gacaacca
//
LOCUS       DAJTVE010000230         4130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000230 DAJTVE010000000
VERSION     DAJTVE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..528
                     /locus_tag="OO439_004083"
     CDS             115..528
                     /locus_tag="OO439_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6318298.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO439_004084"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO439_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6318299.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO439_004085"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO439_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="OO439_004086"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="OO439_004086"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6318300.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO439_004087"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO439_004087"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6318301.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO439_004088"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO439_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6318302.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="OO439_004089"
     tRNA            complement(3794..3881)
                     /locus_tag="OO439_004089"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1100 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa
//
LOCUS       DAJTVE010000231         4080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000231 DAJTVE010000000
VERSION     DAJTVE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..788
                     /locus_tag="OO439_004090"
     CDS             <1..788
                     /locus_tag="OO439_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6318303.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            801..1761
                     /locus_tag="OO439_004091"
                     /pseudo
     CDS             801..1761
                     /locus_tag="OO439_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1799..2698)
                     /gene="nhaR"
                     /locus_tag="OO439_004092"
     CDS             complement(1799..2698)
                     /gene="nhaR"
                     /locus_tag="OO439_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6318304.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2764..3930)
                     /gene="nhaA"
                     /locus_tag="OO439_004093"
     CDS             complement(2764..3930)
                     /gene="nhaA"
                     /locus_tag="OO439_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6318305.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1227 a          953 c          861 g         1039 t
ORIGIN      
        1 atattgccaa aacacgtcac caaattaact tatcgaattc gacctcattt tccaaagatg
       61 gctattcctc caacaatact ggaattactg gcatagccgg tgaacatgat cagttaaatt
      121 atggtatata tgttaatcag caacaacaaa ataatgatac ctcgcttggt acgaatttaa
      181 gctggagaac tcccatcgcc ataatagatg gcagctatag tcattctaaa aacgcctggc
      241 aaagtggtgg aagtattagt agtggattag ttgtctggcc cggtggtatt aatatcacta
      301 accagttatc cgatacattt gcaattctgg atgcgcctgg gttagaaggt gcacatatta
      361 atggacaaaa atacaatcga acaaacagca aaggccaggt tgtttacgac ctgataatac
      421 ctcatcgtga aaaccatctg gtacttgata tagcaaacag tgaaagtgaa acagaattgc
      481 agggcaatcg tcaaattatt gcaccttacc gtggagcagt ttcttatgtg cagtttacaa
      541 ctgaccaacg taagccctgg tatatacagg cactgcgtcc cgatggttcg ccattaacct
      601 ttggctatga cgtactggat ctccaggaaa ataatattgg agtcgttggc cagggtagtc
      661 gcctttttat tcgcgtagat gaaattccca ctggaataaa agttgctctc aatgatgaac
      721 agaacttatt ctgtactatt acttttcaac acgttatcga tgaaaacaaa acatatatat
      781 gccagtaaaa ggagtttact atgaagtggc ttttattaat caccttgagt ctatattctt
      841 ttattgtgca aagtgctccc tgtgcattga ccaatgtcgg ggaacaacgt ggaacatata
      901 tactcaagcc actgagtatg aaaggtaatc tcaccgccga aaaaattgaa aacaccggaa
      961 atatcattga gtgtgcagac ttacaagaga cgaccaacgc cattaaagtt cattttttaa
     1021 cacagaacgc tttaaggttt aaagtcggaa gtcgtcatta tattattaat ttcccctttg
     1081 aatctgacgg taaaaaaaat agagcttcca ggaaggtcat cttataattt agaagaaatg
     1141 ctatctgcga taaatttcac cattccatac tctatcgcat ctgtatcaga tagttccaac
     1201 accgcagata tttttccagg aactcctttt cctttgttgt tcgaaattga agtgagttca
     1261 tgtacaggga ataatactaa ttgtaatagc gtccgcttta attataattt aaatactatt
     1321 ttacaggtaa atttaatgac ctgtggtttt gaagatcaat ccatcgacac tggacgattt
     1381 gtactgtctc gtatcaataa tgaaaattat cagtttcatc atataagatt tgactgcatc
     1441 cagggcgagc aaggtgaatt agtttttgaa cccagtgatg ttgaatatta ctttgaacca
     1501 gtgacgatac aggagtcagg aaccagcata ttaaaaaatg agttagaaaa cagtgaggac
     1561 ggtgcgggtc aagtaggctt tcaaccctcc aatgatggta cgaatgaaat ccagtatggt
     1621 aaaagcaatt actactcttt tcagcatccc catgaaggaa gtaatcagat tcctctattt
     1681 attcgcccgc gtacatatgg aaataatgtc tcaagcgggc aaataatgag ccgagtcaaa
     1741 atcgtggtta tgtacaacta atctatctcc cccaccttaa ctttggggga ggtgccgatt
     1801 aacgcgccgc tggactaaaa agcgcagaat aatccgtatt gcagattcgc tgtaccgccg
     1861 ggtgctgaat catccgctca gcaaaaatag catggtactc ttccatcaca ttctcgacgc
     1921 gaccaatttc tacgacagtt ttatcggcat aaaagtcata tgcataaagc gttggggcaa
     1981 cgaagattgc attgtgcatc gcaccaaaag ctttcatcaa cgcggcatca tcaaactcgc
     2041 cgaggatttc tacgtttaat ccctgggagt taaaccagtt aagcaatttg cgccctaaca
     2101 ttgaacgtcg cccaggaatc aaaagtcgcc gttcttccag acaagccggg aacgattttt
     2161 ctggtggtgg atttgtacac cagaaactca cgccacattc gccaattctc acggagaaca
     2221 ggccttcctg ctgcgtagag tctatcggac agtcagaaat gatcatatcc agtttatgct
     2281 gacttaattg ctccagcagc atttcgtggg tggattcgaa gcagcgaaga tgaatgggct
     2341 cgccttctac cactgcggcg ttaagtacgc tactgaccag gcgtttggaa agtgcatcag
     2401 ccacgccaac gtcaaacaat aaattggatt ctttgcgata gttcacaata tccagcattt
     2461 cctggcttaa ggtgaacatt ttatcggcat agcgatagac cagttctccc agctcgctgg
     2521 gttcgagacc acgtcccttg cgtttaaata atttgccttg caggcgctct tccagcgctc
     2581 gaatctgtcc ggtaatggtt tgtggagtta aataaagcgc ctccgctgcg ccaaccacgg
     2641 aaccttcttt atagacatgc cagaagtaat acaagtggtt gtaattgata tgagacatgc
     2701 tcatttctct ccctgataac aatgaaaagg gagccgttta tggctccccg gtaaaccgtc
     2761 ctgtcaaact gatggacgca aacgaacgcg taaccagctg tatccaatta ccgccgaaga
     2821 gatagaaccg accaggatac cgagtttcgc ccagttaatc agttctggat ctacgctacc
     2881 aaaggccagg ctggcaataa agatagacat agtaaaaccg ataccgcaca ggatccccac
     2941 cgccataatt tgctgataag tcgttccctc aggcagatgc gccagtttca aacgcagcgc
     3001 caaccagcag aacagactaa tccccagcgg tttgccaatc agcaagccag cgatgatccc
     3061 caatggcaga atggaggtca agccatccag cgtgacgcct tgcagtgaaa cgccagcatt
     3121 agcaaatgca aacagcggta aaatcagata cgccacccac gggtgcaata catgctccag
     3181 tcgcttcgct ggagaacgcc catgcttctc tttcaaagga ataaagaagc cgacaattac
     3241 ccccgccagg gttgcgtgaa cccccgattt caacaccgca gtccacaaca ccacgccaac
     3301 aagaatatag acgcccgtgc ggcgtacacc acacagattc aataccgcga gtaccgcaat
     3361 tgctacagcc gcgacgccaa gagaggccat cgataagtca ttagtgtaga acaatgcgat
     3421 gataatgatg gccccaagat cgtcgataat agccagagcc atcaaaaaga tcttcagtgc
     3481 taacggaaca cgacttccca acagcgccag cacaccaagt gcaaaggcaa tgtcagtagc
     3541 cgccgggatc gcccaccctt cgcgggtaat cggatcggca tagttaaagg ccagatagag
     3601 taatgccgga acaatcatcc caccaatagc ggcgataacc ggaaatgcgg cctggcgtaa
     3661 gctggctagc gatccttgca tcagttcacg tttaacttcc agaccgacta acaggaaaaa
     3721 taccgccatc agcgcgtcat ttatccataa cagcatgttt ttgttgattt cgagtgaacc
     3781 aacccggagc tgaaccggcg tctccagaaa gtcgtgatac catccactgg ttgcgccgct
     3841 gttggccatc atcatcgcca ggatagcggc aatgataaga ataatgcctc ccgaggcatc
     3901 actgctaaag aatcgatgca gatgtttcac tttttatttc tctttcaggt gaatagatcg
     3961 tttttactat tttaccccgc acaaatcatc gttaaaatca gattatagtg taggaataac
     4021 tcgtttttta cgagcaatta aaggatttgc gccatcagcg gtgagatgag tttttgacgg
//
LOCUS       DAJTVE010000232         4059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000232 DAJTVE010000000
VERSION     DAJTVE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            36..1182
                     /locus_tag="OO439_004094"
                     /pseudo
     CDS             36..1182
                     /locus_tag="OO439_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1476..2717
                     /gene="agp"
                     /locus_tag="OO439_004095"
     CDS             1476..2717
                     /gene="agp"
                     /locus_tag="OO439_004095"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6318306.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2755..2982)
                     /locus_tag="OO439_004096"
     CDS             complement(2755..2982)
                     /locus_tag="OO439_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6318307.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3003..3608)
                     /gene="wrbA"
                     /locus_tag="OO439_004097"
     CDS             complement(3003..3608)
                     /gene="wrbA"
                     /locus_tag="OO439_004097"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6318308.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3660..>4059
                     /locus_tag="OO439_004098"
     CDS             3660..>4059
                     /locus_tag="OO439_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318309.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1145 a          865 c          955 g         1094 t
ORIGIN      
        1 gcgtcattat ttattaaatt ttatagacgc tatatatggg tagtaatata catggaatta
       61 gttgcactgc aaataattat ttgaaacagg cctggaacga tataaaaaat gagtacgaaa
      121 aaaatcaaac atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttat
      181 acaatgaact cagacgtaaa gtaaatgaag aggatactcc atgtctggaa tgtgaatcac
      241 tagaaaaaga atttgaggaa atgcagaatg ataatgatct atcattattt atgagaacat
      301 tgcgtactaa tgatacacaa atttattcag gggtttcagg aggtattaca tatactatac
      361 aatatgttca aaatgttgat attgttagag tgtccttgcc gggcagaggt tcagagtcta
      421 tcacagattt taaaggttat tattggtatg gctttatgga atatattgaa aacattactg
      481 cgtgtgatga tattttttct gagtattgtt tagatgatga aaatatgagt atccagccag
      541 aacagataaa tacgccggga atatctgatt tagatactgg ttttaaaaga acatgagtat
      601 tcatgggcgc tgggcggcca taagacgcaa aagcaagtat acgaagagtt tattaaaatt
      661 gttgataaac aaacgatcga ggctcttgtt gatacggcat tctataatct cagggaagat
      721 gtaaagacgt tatttggcgt taatctacaa tctgacaaca aaattcaggg gcagggtggt
      781 tttttatcat ggagtttttt gtcttttaaa aaagaattta ttgatagttg cttgaatgac
      841 aaaaaatgca tactgcattt acccgaattt atatttaatg ataacaaggc acgccttgtt
      901 ttagacaccg acccagagca gaaggttaat gaggtgaaaa atttcctcac tgctctttca
      961 gatagcattt gctcattatt tattgtttac agcaatgtgg catcaatctc cttagggaat
     1021 gaatcctttt caacagatga cgaccttgag tatgggtatt taataaacac tggcaatcat
     1081 tatgacgttt acctccctcc tgaacttttt gctcaggctt acgagttaaa caataaggaa
     1141 aggaatgcgc aaattgattt tttaactcgt tatgcaattt aatgacaaag gcatgtgcta
     1201 aaaaccattg ttattagcct cacttctctt attattggta ctattgcctc tgtattagta
     1261 acgccgctga tattctgttt atccacaggt gcaattatca gcggcgtacg cgaggcaggg
     1321 gctaatcagg catagtttgc gtcaaacctt gcctgttttt gaagatgtat atagaaaaac
     1381 aggcgttcaa caagccattt tgcgaacctg ttcccggaaa aaagtcatat ttctgtcaca
     1441 ctctttaatg attgataaca aaagaggtgc caggaatgaa caaaacgcta atcgccgcaa
     1501 ctgtggcagg gatagtttta ctcgcttcaa atgctcaggc acaaaccgta ccggaaggct
     1561 atcagctaca gcaagtgctc atgatgagcc gccataactt acgtgcgccg ctggcgaaca
     1621 atggcagtgt gctggagcag tcgacgccga ataaatggcc agaatgggac gtccccggtg
     1681 ggcaactcac caccaaaggt ggcgtgctcg aagtgtatat gggccattac atgcgtgaat
     1741 ggctggcaga gcaggggatg gtgaaatcgg gggaatgccc gccgccggac accgtttatg
     1801 cctatgccaa tagtctgcaa cgtaccgttg cgaccgcaca gttctttatt accggcgcat
     1861 tcccggggtg tgatattcct gtgcatcacc aggaaaaaat gggcactatg gacccaacct
     1921 ttaacccggt gatcaccgat gattccgccg cattcagtga acaggcggtg gcggcaatgg
     1981 agaaagagct cagcaaactc cagcttaccg acagctacca gctactggaa aaaatcgtta
     2041 actataaaga ttcccctgcc tgtaaagaga aacaacagtg ttcgctggtg gatggcaaaa
     2101 atacctttag cgccaagtat caacaagaac caggtgtttc cgggccgctg aaagtcggca
     2161 actcgctggt agatgcgttt actttgcaat attacgaagg ttttccgatg gatcaggtgg
     2221 cctggggaga aatcaaatct gaccagcagt ggaaggtgtt gtcgaagctt aaaaacggct
     2281 accaggacag cctgtttacc tcaccggaag tggcgcgcaa tgttgcgaaa ccgctggtca
     2341 gttatatcga caaagctctg gtcaccgatc gcaccagcgc accgaaaatt acagtgttgg
     2401 ttgggcacga ctccaacatt gcctctctgt taacggcgct ggatttcaaa ccgtatcagt
     2461 tgcatgacca gaacgaacgc acgccgattg gcggcaaaat cgttttccag cgttggcatg
     2521 acagcaaagc caatcgcgat ttgatgaaaa ttgaatatgt gtatcagagt gcggaacagt
     2581 tacgtaatgc cgatgcgtta accctgcagg cacctgctca gcgtgtgacg ctggaattaa
     2641 gcggttgccc gatagatgcc aacggtttct gcccgatgga taaatttgat agcgtgttga
     2701 atgaagcggt gaaataacag aaaactcccc cgcgagaagc gggggagtcg ctggttaaac
     2761 gtttttacgt tcgatggtct gttcgcccca aaaaagcgaa tctttatcgg ccttagcaaa
     2821 ggctttgact aacacttcat cgctaccttc ttcccaaatc ttttctgcca ttttttcgtc
     2881 gtacccggcg acttcgaaaa tggcctcggc tatttccggc gacgtattgc gcagagatgc
     2941 ccattcaccg acgtgatgag ctttcgcttc ttgagttggc atgcgtatcc tcctgttgaa
     3001 gattagccgt taagtttaac tgccagacct gcgacatatt ccccttgata acgagcaata
     3061 gacagttctt cctggcttgg ctggcgtgag ccgtcaccgc ctgcgatggt ggttgcgccg
     3121 tacggcgtac cgccgcgaac ctgtgaaacg tcaaataatt cctgcgctgc gtagccaatg
     3181 gggacaatca ccatgccgtg atgcgcaagg gtcgtccagg ttgaagtaat agtttgttcc
     3241 tgaccgccgc cagtaccggt ggaactaaag acgctcgcca gttttccgta tagtgcgccg
     3301 gaagcccaca ggccgcccgt ctggtcgagg aaggtacgca tttgaccgga catgttgcca
     3361 aagcgggtag gtgtaccaaa aataatggcg tcgtaatcgg ccagttcttg cggggttgca
     3421 accggtgcag tttgcgtttt accgcctgct ttttcaaata attgcggcgg catggtttcc
     3481 ggtacacgct taacgacaac ttctgcgcca tcgactttgc ttgcaccctc agcgactgcg
     3541 cgtgccatcg tttcaatatg tccgtacatg gaataataaa gcactagtat tcttgcctta
     3601 acactcatat cattgttctc ctgttcaatg aaccacttag ataagcatag tccgaagata
     3661 tgaagctgct agtgacatac atcaggtgga gtactaaaga gcaagactca ggagactcct
     3721 taagacggca aacaaacctc attgatgctt tctactctaa gcacaaaaac gattattacc
     3781 ttcttcctgc tcacagatat gttgataagg gtaaatctgg ttttcaccag caacataaga
     3841 atcaaggttc tgacttcaga cgtatgtttg aaaatgttat gtctggggct attccagaag
     3901 gttccttgat tgttgtagag aactttgacc gcttcagtag ggctgatatc gacacagcta
     3961 tagatgatgt caggcagata ctgaggaagg gggtctctat actgactctg ggtgatggtg
     4021 aactctacga caagtcagcc ttaacagacc ctgtgaagt
//
LOCUS       DAJTVE010000233         4052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000233 DAJTVE010000000
VERSION     DAJTVE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..3223
                     /locus_tag="OO439_004099"
     CDS             <1..3223
                     /locus_tag="OO439_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6318310.1"
                     /translation="QVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEG
                     LDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKA
                     QTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDR
                     QGRLAEETSRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYT
                     TRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTAIVA
                     PDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLT
                     EQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDH
                     GWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLA
                     TRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAW
                     NTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANL
                     DIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIP
                     EGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERD
                     LTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKA
                     RHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQ
                     LEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLR
                     LPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLK
                     FHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIYW
                     NSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEAR
                     VITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            3235..3684
                     /locus_tag="OO439_004100"
     CDS             3235..3684
                     /locus_tag="OO439_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318311.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            3946..>4052
                     /locus_tag="OO439_004101"
     CDS             3946..>4052
                     /locus_tag="OO439_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR6318312.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICA"
BASE COUNT         1072 a          891 c         1267 g          822 t
ORIGIN      
        1 gcaggttcgc gggtttactt atgatgcgga gcacgccggg cggatggtgg cgcaccatta
       61 tgcggggagg ccggagagcc gctaccggta tgatgatacc ggtcgggtga cggagcaggt
      121 caacccggag gggctggact accgctttga gtacggagag agccgggtaa tcatcacgga
      181 cagcctgaac cggcgggagg tgctgtacac ggaaggcgag ggtggcctga agcgggtggt
      241 gaagaaggag catgccgacg ggagcatcac ccgcagcgag tatgatgagg cggggaggct
      301 gaaggcgcag acggatgcgg cggggcggcg gacggagtac cggctgcata tggcgtctgg
      361 aaagctgact tcagtggtac tgccggacgg caggacggta cggtatggtt acaacaacca
      421 gttgcaactg acgtcagtga catatccgga tggtttacgc agcagccgga aatatgaccg
      481 tcagggcagg ctggcggaag agacctcgcg caacggtaac attacgcgct ggttttatga
      541 ttcttcccgc agtggcctgc cctgtgcggt ggaagacgga accggggtga ggcggagaat
      601 aacgcgaaac cggtacggtc agcttcaggc gtttacggac tgctcggggt acacgacgcg
      661 gtatgagtat gaccggtacg gtcaacaaat cgccgttcac cgggaagaag gcatcagcac
      721 ttacagcagt tataacccgc gcggtcaact ggtcagtcag agggatgcgc aggggcgtga
      781 aacccgttat gagtacagcg ccgcaggaga cctcaccgct atcgttgccc cggacggcag
      841 ccgcagtgag atacagtatg atgcgtgggg aaaggccgtc agcaccacgc agggcggtct
      901 gacgcgaagc atggggtatg acgccgccgg gcgcatcact gtgctgacca acgagaacgg
      961 cagccagtcc acgttccggt atgacccggt cgacaggctg acggagcagc gcggctttga
     1021 cggccggacg caacgttacc agtatgacct gaccggaaaa ctcacgcaga gtgaagatga
     1081 ggggcttatc accctctggc attacgatgc gtcggaccgt atcacccggc gcacggtgaa
     1141 cggtgaaccg gcggagcagt ggcagtatga cgaccacggc tggctgacag aaatcagcca
     1201 cctgagtgaa ggccaccgtg tggcggtcca ctacggctat gacgataaag gccgtctgac
     1261 gggcgaacgg cagacggtgg agaccccgga gacgggggag atgctgtggg agcatgagac
     1321 ggggcacgcg tacagcgaac aggggctggc gacccgtcag gagccggacg gcctgccgcc
     1381 ggtggagtgg ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg gcggaacacc
     1441 gctggtcgag tacacgcggg accggctgca ccgtgagacg gcccgcagct tcggcgggga
     1501 ggcatatgaa cttgccaccg cctggaatac cagcggccag ctccggagca ggcacctgaa
     1561 ccttccgcag cttgaccgtg actacgactg gaacgacaac ggacagctaa tccgcatcag
     1621 tggcccgcag gagagccggg agtaccgtta cagcgacacg gggaggctga cgggcgtcca
     1681 caccacggca gcgaacctgg atatcgatat cccgtatgca acagacccgg caggaaatcg
     1741 gctgccggac ccggaactgc acccggacag caccctcacg gcgtggccgg ataaccgcat
     1801 cgcggaagat gcgcactatg tctatcgcta cgatgaatac ggcaggctgg cggagaagac
     1861 ggaccgtatc ccggaagggg ttatccggat gcacgacgag cgcacccacc actatcacta
     1921 cgacagccag caccgcctgg tgttctacac gcggatacag cacggcgaac cacaggtgga
     1981 gagccggtac ctctatgacc cgctgggccg ccggacggga aaacgggtat ggcggcggga
     2041 gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg gaggtgacat ggtacgggtg
     2101 ggacggtgac aggctgacga cgatacagac cggcaccacg cgtatccaga cggtatacca
     2161 gccggggagc ttcacgccgc tcctgagaat cgaaacagag aatggcgaac aggcgaaggc
     2221 gcggcaccgg agcctggcgg aggtgttgca ggaggacacg ggtgtgacgc taccggcgga
     2281 gctgtcggtg atgctgggga ggctggagcg ggaactgcgc gcgggagcgg tgagtgcgga
     2341 gagtgaggca tggcttgcgc agtgcgggct gacggcggag cagatggccg cgcagctgga
     2401 ggcggaatac atcccggaga ggaaacttca tctttaccac tgcgaccacc ggggactgcc
     2461 gctggcgctc atcagcccgg aaggggaaac ggcgtggcag ggggagtatg acgaatgggg
     2521 aaacctgctg ggcgaaacca gcgcgcagca cctccagcag tcactccgtc tgccggggca
     2581 gcagtatgat gaggagtcgg ggctgtacta caaccgcaac cggtactatg acccgttgca
     2641 gggaaggtat atcacccagg atccgatagg gctgagaggt gaatggaatc tgtataaata
     2701 tccattaaac cctgttcggt ttatagattc tttgggttta aaattccatg ttaacggtga
     2761 tccaagtgat tttaatcagg ctgttgagta tttaaaacaa gattcacaaa tgaaagaaac
     2821 tatagatttt ttatcaagct ctgaagaaac tataaatata gagtatattg aaggaaccaa
     2881 tgttagattt aattcgaata atatgacgat atattggaat agtagagcat ctttattttg
     2941 ttcaactgaa ttaaactcga aatcacaatc gcctgcatta ggtttgggac atgagtttac
     3001 tcatgcgcag tattgccttc ttgataagga aaatttcatg gctcttttaa gccgaacaga
     3061 taagaaatat gagaataaag aagaggcaag ggttattaca ataattgaat cgcgtgcggc
     3121 gaaaacttta ggtgagtgta ccagaggagc tcatagtgga ctgccttttt atcgggtgga
     3181 tggaccgttg cagacgatga aaataacggg tacgccagaa tagtgaggtt ttttatgatg
     3241 aaatatttga tggttttatt gtctctattt tctggttcgg tattggggat ggggcgagtg
     3301 aatgaattgt gtggaataga tagcgtgaaa acgatagaga taattaatct tccatcgtat
     3361 gttaccacgt tagttccgct tagcaaggaa ggtcttaatg aaatatacag atataaagtg
     3421 gtggtgaatg aaattagaga tctttatgct ggtaagataa ttgatttatt gcaaatgaag
     3481 tatttcagaa aagaaaaata taacaatata agatgggggg gtagtatcat ttcgaaaggt
     3541 aataataaat gtgaaattta ttttgatgct tttggtgaat gtggaagtgt gaatggcatt
     3601 aacgtgtgtt ttgaaaaaaa tgaaatgata ggttggatta aaaaagaaat ccctttgctg
     3661 tcgcaaaaaa taggtgggct ttaaatggta gggcaatatt caaagacagt ataggtaact
     3721 atttgtatgg ccatgtaagg taaaagaaaa ggaataaacc atgagcggaa aaccggcggc
     3781 gcggcagggc gacatgaccc gcagggaaag atcatcagaa gttcactttt tgtactaaat
     3841 aattcgcatt ttatgtttaa aaattgagat attccttatt acctaaagct gttttttatt
     3901 gcttatacat gatcaaatac ttcttacata attaaggaga acaaaatgga acttaaaaaa
     3961 ttgatggaac atatttctat tatccccgat tacagacaag cctggaaagt ggaacataaa
     4021 ttatcggata ttctactgtt gactatttgt gc
//
LOCUS       DAJTVE010000234         4027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000234 DAJTVE010000000
VERSION     DAJTVE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..869
                     /gene="adeQ"
                     /locus_tag="OO439_004102"
                     /gene_synonym="yicO"
     CDS             <1..869
                     /gene="adeQ"
                     /locus_tag="OO439_004102"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6318313.1"
                     /translation="IALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLS
                     SRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGII
                     FSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTA
                     YIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSL
                     ARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVV
                     ASLFALKIILVD"
     gene            922..1374
                     /locus_tag="OO439_004103"
     CDS             922..1374
                     /locus_tag="OO439_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6318314.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1585..2765
                     /gene="nepI"
                     /locus_tag="OO439_004104"
                     /pseudo
     CDS             1585..2765
                     /gene="nepI"
                     /locus_tag="OO439_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2806..3099)
                     /locus_tag="OO439_004105"
     CDS             complement(2806..3099)
                     /locus_tag="OO439_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318315.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3321..>4027
                     /gene="nlpA"
                     /locus_tag="OO439_004106"
     CDS             3321..>4027
                     /gene="nlpA"
                     /locus_tag="OO439_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6318316.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAR"
BASE COUNT          876 a          900 c         1064 g         1187 t
ORIGIN      
        1 ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa gacacgctgg
       61 tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg ttttttatta
      121 taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt gtggtgacgt
      181 cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc attccgcctg
      241 atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt gaactcgccg
      301 gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca ttaattggtg
      361 taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg aataaggcgc
      421 tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg tctgttactg
      481 cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg actgcggttg
      541 tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg atggttcctc
      601 cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg agcctggcgc
      661 gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg gtgatgatgc
      721 cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac tgcatcatga
      781 aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc gcatctctgt
      841 ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga tatcatgagt
      901 tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc ggtagtgttt
      961 gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg tctcagtgat
     1021 cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg aaagtcagcc
     1081 ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca aaacgccgcg
     1141 caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa
     1201 tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc tcaggtcagg
     1261 ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag
     1321 atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca ttaaaaccgc
     1381 ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac gttcacgccg
     1441 catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag caacaccgta
     1501 aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc ggcttttccc
     1561 ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg cggcgcggat
     1621 gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt cgcctgtctg
     1681 attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga tttaggcatt
     1741 tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat gtttgccagt
     1801 ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat tttgtttgcc
     1861 gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag tttgctttta
     1921 atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc ggcgtcgctg
     1981 accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat cttcggcgcg
     2041 gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga gcttatcggt
     2101 tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt ctggattatc
     2161 aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac tttccgctta
     2221 ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt cgccgggcag
     2281 tttgctttct tcacatatat tcgcccggtg tatatgaacc tagcgggatt cagcgtggat
     2341 ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac gtcgctttcg
     2401 tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt aatactggct
     2461 gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac cggcgtggcg
     2521 attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg gatcacccgc
     2581 tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat tcagcttgtg
     2641 gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg ttgatgtttt
     2701 ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa atgaagaaat
     2761 cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca tatccgggca
     2821 ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca tcgccacgat
     2881 ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt cccacgcttc
     2941 atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt
     3001 ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc ctggcatagc
     3061 gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct tttacaaatg
     3121 tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac
     3181 gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt cctttttatt
     3241 cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag tcaggtaatc
     3301 attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca ggggccgcat
     3361 tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca
     3421 ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg
     3481 caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg ttgctaccga
     3541 acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga ccctttcttg
     3601 aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg tttgtcttcc
     3661 ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa ggcgcgacag
     3721 tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg caaaaagaga
     3781 agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat ataaccgata
     3841 atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc gtactggatg
     3901 atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca gggctttctc
     3961 cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat attttggtgg
     4021 cacggga
//
LOCUS       DAJTVE010000235         3998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000235 DAJTVE010000000
VERSION     DAJTVE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1709)
                     /locus_tag="OO439_004107"
     CDS             complement(<1..1709)
                     /locus_tag="OO439_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6318317.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYD"
     gene            complement(1794..2129)
                     /locus_tag="OO439_004108"
     CDS             complement(1794..2129)
                     /locus_tag="OO439_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6318318.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2129..2485)
                     /locus_tag="OO439_004109"
     CDS             complement(2129..2485)
                     /locus_tag="OO439_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6318319.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2485..3981)
                     /locus_tag="OO439_004110"
     CDS             complement(2485..3981)
                     /locus_tag="OO439_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6318320.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          901 a         1219 c          887 g          991 t
ORIGIN      
        1 atgtcataga caaaatcatg gaactgtttg ccccactcgt cgaacttatc attcagacca
       61 aatccactgt tcagtgcatc agccacaaaa tttttaacaa gccagggatg tgctttttca
      121 aactcctgcg ccatcatgcc cagttcaagc aatccaccaa tcagactgat ctttccgagg
      181 ccctttaatt tcagccctgt ttttcctgcc gccgttgtcc attgattctg ggcgactgtt
      241 gccgcacgaa tacttgccac cgccttcaca ccaatataca gggcagaaac cgttgctatt
      301 gtttttactg cctgttccca tccccccatt gctctgacaa cctgatcaac ctcctgccag
      361 acttttttca caaccggacc gaccgtttcc cagttatcaa ttatgagata tgcaccaccg
      421 acgagaagcg caattaatcc ctttgccggc gtcatattca tcacgctacc catgattttt
      481 gttatgcgtg tcaatgtgcc aatcgccacg cccatcgtca gtaatgccgc accagttttc
      541 gcgatggttt ttacgacctc aggattttct ctgacaaacg tgcgcacttc ttccagaaaa
      601 ggcttcatct cttttatgcc ttcattgagt gaaggcagga aggtttcccc cagcgtggaa
      661 gaaatcgcat tgatctggtt ctgcagcagc aacaactgat tttctgtcgt cgcggcacgg
      721 gcggcatatt ctttctgcat tgagccgcca tactgctgcg catccgccac acgattaaag
      781 ttggttcgca acaaatccag attcgtgagc agcggcgcaa tggcccccag ggattccttc
      841 ccgaacagcg ctttcagcac agcagcctgt ttttctttcg gcacttttgc cagagaatcc
      901 agtacgtgca gcatggcccc gcgggcgtct ttctgcatat ccgccgccag ttttttcggg
      961 ctaatgcgca acgagcggag cacttttttc tgcgatttgg tggcagaatc cctcgctgtc
     1021 agcgacaaca taaaattctt tatccccgtc gcagcaattt ctgactccac ccccattccg
     1081 gcaatggttg cccccattgc ggcaatctct ccggaggcca cacccgcaac actgcctaaa
     1141 gggccaatac gggtcacaac atcagaaatc tttttcgcac ttgcaggacc ggtattacca
     1201 agatagttaa tcttatccgc aagccctgcc acctctccct gtgtcagttt aaaggcagtg
     1261 cgccactgcg ccatcatctg accggattct tctgccgtgg tgtcaaaggc cacgcccatc
     1321 ttcacggcat cgtcagtaaa ctgcatcagc tcgtcacggg cgataccagc ctgaccaccc
     1381 gccgccacaa tttcggcgat accttccgcc gacatgggca gttctgttga caggtcgcgc
     1441 acctgctccg tcattgcctt aaacgcttcc ggcgtatcca gaccatccac cactttccgg
     1501 acatccgcca tttttgattc aagggcaatg gcagatttga ccgggagcgc cagcgccccc
     1561 agtactgcgg ttccggcacc cgctgcgccc agtaacaggc tggcaaactc ctttttaaaa
     1621 cctttcagct ggcgctgcat tcccttcagc ggtgccgata ccttatccac ggcagtgatg
     1681 atcgccttca gctgaaaact gtcagccatg cttcatctcc tcgttaatgc ggacggcctc
     1741 ggcttccagt tctgtaaact gcgaaacagc cccccgtcgc agctccagtg gattcagttt
     1801 ccagaaccag gcaacattgt agagtcgttt ccggaggtgc ttcccgtctc cgactgggta
     1861 aaaaaacgca ggatctgcat gctggtctta aaaatatcca gttttgccat ctgcgccgcc
     1921 gatgaacgcg ggatccccgc cagcaacggg atatatttca gtgccacctg gctgtccagt
     1981 ttaatactgc cttcaccgga aataatgaaa ggaaaaccca gcgcctcgat ttcgtcatac
     2041 gtgggctcgc gcaactccag cacatgcagc atttcgttat gcgctgtcac cggttttttt
     2101 aaaactattt ctgtaacgtt cattgccatt actgataccc tccctcttcg ccgtggaatt
     2161 caagatctgc cgtgccttct tcggcattat gattagcctc tccgtgcagc catgccgccg
     2221 aaagcacata caccatgccg tttgccagct cagcggtaat cgtcatctgg tctgaggtgg
     2281 taattttgtc gaccggaaag tttttgggca ctttaaacgt gcccttaacg taaggtgacc
     2341 gccaggtctc cttgtaatcc acatcccctg ccatgccgac gatgtcatcc cggacattgg
     2401 tgttcatcgg cacttcaatg ccacctgtca gcgataactg ctgaccatcc actttaaaaa
     2461 aacaggttcc ggcgatcttt gccattatgc tgactcctct gaatactgaa gacggaactg
     2521 gttaaccacc gcaaagacac gtaactgatt aacataatcc ggcgggaaca gcgtattcag
     2581 tcggttcggg ttatctgcat cacgctcaac tatcagatac tgtttaaaca gatcgtaatt
     2641 ttccacaata ccggcacgct ccatctgacg atatgtcgcc agaagttccc ctttgataac
     2701 ggcaggagtg acaatcgcct gccccggacc aaaacgggta ccatcatttg ccagcttgtg
     2761 acgtccgtac ttgcttgtga tgaccgattt cagtttgcga agaacatatg cactggtatg
     2821 cagagtttca ctgtccagat agctgttgtc tgccacgcca tacgcattct ttttgtaggt
     2881 ggttacggaa cgctggatct gcaacgtgcc gccttccaca taagccgtcg ccacgccgtg
     2941 agataaaagg gtctgctgct ctgtcatgat gaatcgctta cctttcggtg ccggaagcat
     3001 ccccaccagc tcccccgtct gtgtcggacg ggcaggatca ttccggataa ataccgcttc
     3061 acgggcaagg cgactggcaa ccagttcatc gacaggcgac tgggtttctt tttcgtaacc
     3121 ggcaagcgtg atatgttgct gattatgcat atctccggca ccaaccagct ctgacagcgt
     3181 tcccagtttt gcggtatagc catgcccgta taactggcgc gcataactcc agcgaccgct
     3241 gctgtcattc atttcggtca tcatcatatt gatggaggcg gcatcgttga acggcagacc
     3301 gataaagtcg aatgcctcat cgcccatagc agcaacagcg gcggtaagat caggcgctcc
     3361 gctaccggca gcaccgactt ccgtcaccac ccgaagccct gctggcagaa tctcaccacc
     3421 accagaacca taataattca gacagaccgg taactcatta ccatacagcc ccttatgacg
     3481 ggcagtcagc gtcaccacac ctgcatcaga tgacgccgta aacggcaggg tgataacccc
     3541 gtttaccgct tccttaatcg cggtggcaac cgcagtggca tcatcgccat tcaccacagg
     3601 cacctgtaca cgggagcgcc cgacatacag actcagggtg ccgctttcct ctgcttctcc
     3661 ggcaaccgtc accctgaccg tcgccgccgc ccctctggct tccggtactg caataacata
     3721 cagttcaccg aaaggatctg tctgacggta gacttccacc atacgcgcca gctggctccc
     3781 cgccccacaa atctggcggg cataatctgc cgatggcatc agcaccaggc tgttaacctc
     3841 aatggctgca tcgttgctgg catgcccgat cagtaatgca ggcgcgctgg tcaccgctgt
     3901 atttgccgca gaattgttca tctcggcgta gaacaacggc accagcgtat tcgacggaac
     3961 agtattaaaa cttatcgtca tgatttttta gccttatc
//
LOCUS       DAJTVE010000236         3994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000236 DAJTVE010000000
VERSION     DAJTVE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            98..799
                     /locus_tag="OO439_004111"
     CDS             98..799
                     /locus_tag="OO439_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6318321.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1057..3204)
                     /locus_tag="OO439_004112"
     CDS             complement(1057..3204)
                     /locus_tag="OO439_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6318322.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELCRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3383..3637
                     /locus_tag="OO439_004113"
     CDS             3383..3637
                     /locus_tag="OO439_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6318323.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          954 a         1026 c         1024 g          990 t
ORIGIN      
        1 aatccacaga aaaagggggt tgcaaaaaaa aaacttccac ccatatgata agtggcgtac
       61 agtaaaaaaa cgccaccaat agcaacgagc aagcgaaatg tcagatctga tagcttacaa
      121 atcaaacgcc cttatcgagg caagctacaa gctcactttg caggaacagc gcttcctgct
      181 ggtctgtatc agccgcctaa aatccggaga agattcccct tcgccggatg aacaaaaaac
      241 catgaccatc acggctgcag agtttcatga tgcctttcct gacatgggac ggcagcacgc
      301 tgaagctagg ctcaaggaag cgatagatcg cctctgggac aggtcagtaa tcatcaagaa
      361 cgaggaaaaa cgagaagagt tccgctgggt gcagtaccgg gcgcagtatt tcaaaggtga
      421 ggcgaaagta gatatcacct tctccgatgc catcatgccg tatctgacac agctcaaggg
      481 gcagtttacg cgggtagtcg tgaagaacgt ctccagcctt tccagcacct acagcatccg
      541 catttacgag ctgctccagc agttccgcag caccggtgac agaacgatag ctatcgatga
      601 tttccgaacc atgcttgccg ttgaagataa atatcaacaa ttcaaagact tgaataagat
      661 gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg taactgttga
      721 gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg aagacaaaca
      781 gatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct gctgcgtttt
      841 ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa aacgctctga
      901 tgcagcgaaa aaagaccccc caatttttgg gggcggaaaa acaaaactat gcttgcgtta
      961 tgtgagtgtg cttcttgcat tcatccctgt cgatgaacat cgaagaacat cgacgaacat
     1021 aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg
     1081 tctcctctcg gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct cccaggtacg
     1141 attaaaaccc agcgagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc
     1201 aaaaagcggt gcctcacggg ttttcagatg aaactgcatc agttctggtg caggtgatat
     1261 gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc
     1321 ggtcagcgca ctgatgacca gatgcctgtt atcggctgag ggaacggcaa acgcgggata
     1381 cagcgtacca ttaagccgga taacaccgcc cagagagtca tccacgccca tgaccagcaa
     1441 aagctgaagc tggcgggttt cccggtcgac agaactcatg accggcatca gcgtcggtag
     1501 cagacgtccc tgcataaaat ccaccttcct tgccgctggt gatggcatgg ctggctggcg
     1561 gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtatg
     1621 ttgcggccat gtctggcaaa cctgcgtcag cagttgctca aaggttgcag aggaataaac
     1681 acgttgcagt ggcggacgtt ggccgatttc gcttcggtca cagatataac cactcctgtc
     1741 acgcattaaa tcaacactct caaagattct ggtgcggtat ttctccagct cgttccccca
     1801 gagcgtatat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc
     1861 aggcatggga gcctcaccat accccgtaac gactgctctc agcacccctg aatccataac
     1921 ggcattgtca tttttcctga gctgctccgc gcgactacgt tcagtatctt cctgttcctt
     1981 ccggcacaat tcctcttcac gtttaagcaa ctgggctctg agagctgcaa ggccggattc
     2041 cccccactgc tgccactgtt tttcaaatgc catctgactc gcgggaatgt cctgtttctc
     2101 caccttccgg taatgctgtc gtaatttttc catttgctgg ctcagagact ggatttttcc
     2161 ctgctcacta cggtattccg gtgccgccca ttgcatcagg ggaatacggg aaaaaccgga
     2221 cgaaaacggc gatgattttt tcaggctggc agcatggctc atcgcttcct ttcgttcggt
     2281 gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg acatggcatt
     2341 actggtgagt ttccatgccg agttactgtc cttcggcaac gcttcgataa tgtcacgtag
     2401 tgtgtacgaa gttgcgttgt caggaagccc gatattcaga aggtcagcca cacggcgggc
     2461 aggctctgtc agccctccgt tcctgagttc ggtgcgggaa atctgtattt ccaggttaag
     2521 cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc
     2581 acaggcagac tttccgaggt gaataccagg cgttttctca agcccctgtc gctcgtatga
     2641 acgatggtct atctcttcct gataaccggc gttcgccagt gccctgtttg catggtcagc
     2701 ccatgatgcc cgccacgtct cggcatgtgt ccggtcgttc cagtccctga cctttccgct
     2761 gaacccagca ggtccgatag ccctcgtggt cagcatgatg tgcgcatggg gatttgtttt
     2821 atccatgtga tggaacgcca catcagcaat catgccctga ctgacaaagt tttctctgac
     2881 aaaggcgaga acggtttccc gtgcggcatc ctgcggcagt tcgtggggga tggcgagttc
     2941 aatttcccgc gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg cattccagag
     3001 ttcggcacgg ttcgtacacc aggcaggggc ggtggcgggg gtcagaatca cagcctcagc
     3061 cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa gcatcagtga
     3121 accggaacga taggcagcgg aagctacggc gctgtagccg ctgcttctgg agataatttt
     3181 catgctgaga tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt ttctttcagc
     3241 cccgcagggc gcacacaatc gaaacggagt ggagattgta taagtgcgca cttcgtgatt
     3301 tgccgtgcat tatgccagct cagtttttaa actaccagtg acgcgctaaa atgcgatttt
     3361 tcatcgcgca cggagttaca tcatgagcag aacgctcgaa cagaagattg ctgatgctga
     3421 ggcgagatta cagcgcctga aggcgaagag ccggagcctg gacacagcgc agaaggttgt
     3481 tgtgggtgct gcattgctgg caaaggtcag aaagccggag gaggtgcagt tgcgtgcctg
     3541 gttactccag ttcctgaagg cagaggtgac acgacaggct gatgtgacgc gcatactgcc
     3601 gctgattaac gagctggagg cgctgccaga acagtgattg tatcgtccgc tcgccgcagc
     3661 cccagcgacc gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat cagtcgccag
     3721 gttgagcaca tacgcacggt ttttcggcag gtgatatgcg ctgaacccgc atgaatactg
     3781 ggtttccaca gggttggagg gggtgtattc gaaacttgca attctgcttt attgcaacgc
     3841 attgttttta ttggtttttt gttttcagca ccccaagtta tccacaggct cgccgttaag
     3901 tttttaagat cttattttta gatctttttt tttaaaacct ttttaggtct tgtaacacca
     3961 tgattttaaa gatgttctgg caagataagt ggag
//
LOCUS       DAJTVE010000237         3992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000237 DAJTVE010000000
VERSION     DAJTVE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO439_004114"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO439_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO439_004115"
     CDS             1909..3261
                     /locus_tag="OO439_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6318324.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO439_004116"
     CDS             3285..3767
                     /locus_tag="OO439_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6318325.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..>3992
                     /locus_tag="OO439_004117"
     CDS             3811..>3992
                     /locus_tag="OO439_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318326.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTL"
BASE COUNT         1072 a         1029 c         1067 g          824 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca ct
//
LOCUS       DAJTVE010000238         3979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000238 DAJTVE010000000
VERSION     DAJTVE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..506
                     /locus_tag="OO439_004118"
     CDS             171..506
                     /locus_tag="OO439_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6318327.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            499..981
                     /locus_tag="OO439_004119"
     CDS             499..981
                     /locus_tag="OO439_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318328.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1015..1095)
                     /locus_tag="OO439_004120"
     CDS             complement(1015..1095)
                     /locus_tag="OO439_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318329.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1131..1555)
                     /locus_tag="OO439_004121"
                     /pseudo
     CDS             complement(1131..1555)
                     /locus_tag="OO439_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1985..2475
                     /locus_tag="OO439_004122"
                     /pseudo
     CDS             1985..2475
                     /locus_tag="OO439_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2700..3191
                     /locus_tag="OO439_004123"
     CDS             2700..3191
                     /locus_tag="OO439_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6318330.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3526..>3979
                     /gene="ygeH"
                     /locus_tag="OO439_004124"
     CDS             3526..>3979
                     /gene="ygeH"
                     /locus_tag="OO439_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCR6318331.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT         1491 a          635 c          641 g         1212 t
ORIGIN      
        1 agatccataa tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata
       61 cacacaatgc cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg
      121 caaagaattt caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg
      181 catttgtcat aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat
      241 ttttcaagga ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag
      301 atacaattga tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga
      361 ttaactgtaa aaaatatccg tggttatact tccctcttgc aaaatcctca ccggggatga
      421 ttgttatggc atgcaataaa aactataacc aacatgaagt ggcagactgt ttaaccctgt
      481 cttaccgtga ggttaatcgt gattgattat aaaaaaaact tattatttat tttagttttt
      541 atcagtgggt ttattctctt cactgtctat tcttacactg cagaaaaaat gatatataac
      601 gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa tcttaccatc
      661 gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg aacctggcaa
      721 caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg gattgaaaac
      781 tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat ggatcaagtt
      841 gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga aaaaagtgta
      901 agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg taacagagca
      961 ataatgcatt gtgcaaggta aactaaaata gataacaaaa aagatggaac ctcgttaatc
     1021 ataaaacaat aaaattaaat attctcgcag tatatggcag tctaaagcat caaagttttg
     1081 atcaacatct ttcattttag acatctcctt gcaatgtaaa atatcatgta tcaacaacat
     1141 ctggtgcaaa tccatgagtc gaactcgaca ttcatcttag ctcgattatt attccttcgt
     1201 acagtcgatg taaacaatac agaaagagga ttattaagaa ccagtttact caggaatcaa
     1261 tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt aaactaaact
     1321 catcattgac agatcgtgag atataactgt ttttaacttt actctttacg ttgactttat
     1381 tgacagaatt aacattcaca tatcttgaat ttaatgtcca tgatgttgtt tgcgtgagaa
     1441 cattctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc gagtccgaat
     1501 ttacaatatc atgcatatag accatttcat gaatctgcga aaactcaatg tccattgtat
     1561 acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc atcaatacta
     1621 aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac gtctccataa
     1681 ggtgataacc ttgatcaaat gaatttcgaa tataataaag gaaatctttt gagatgttta
     1741 tcacgaataa agctatttat gttaccaata tcatttcgag tgcgcacact atctatctgt
     1801 tttttcgaga tataaaaaac aacgtgtaag ttatgtaaat tgccgatgct actgagtaac
     1861 attatggtaa aaaaagtaaa aatgagcgat ttttgataga ataaagcatg agaaaaggtg
     1921 atatttgaca ttatcagaag ctgcgaattc ggattttgct ctaatcaaat gagagaaatg
     1981 taaaatgaag ccacgaaata ttaataatag cctaccactg caaccattag ttcctgatca
     2041 ggagaacaaa aataagaaaa atgaagagaa atccgttaat ccagttaaaa tcacaatggg
     2101 gtctggttta aattatattg aacaagaatc tcttggagga aaatatctaa cacatgattt
     2161 gtcaataaag atagcggata tttctgaaga gataattcag caagcaatat tatctgctat
     2221 gagcatatat aaattttcga taacagatga tttaatgagt atggctgtaa atgaactcat
     2281 aaaactgacc aaaatagaga ataatgtaga cctgaataaa ttcactacta tatgcacaga
     2341 cgttctatcc cccctcgtca ccagacataa taaagaaaaa aaacaaacga cattctaccc
     2401 ttctcaaaaa tccccttttt aattttcatt gaaaaaatgg caattgatac tatgaatcca
     2461 ggtcatacac attaaggcat ttatgtaatg tcgtaattaa gataactaat aaggtgaata
     2521 ttagtaaaac aactaatgat gttattagtt tgtagtgaac agtactttta ccaataatga
     2581 aaaatatacc ataagcaatg caagcttaaa actaataatt taaaaaagat aaatgtaaaa
     2641 atattaatga gtcaatcaat aataatttag catcacgaga cacatcacag aggaatatta
     2701 tgagcacaga aacaattgaa atattcaata atagtgatga atgggcaaat caactaaaac
     2761 acgcattatc gaaaggagaa aatctggcat tactacatgg tttaactcct gatatccttg
     2821 atagaatata tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa
     2881 tttattataa atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaagattttg
     2941 catcagtatg tcagccaaaa aagaaatatc aacaagcata tgacctttac aaactaagtt
     3001 acaattactc cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg
     3061 gggctaaaaa tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg
     3121 atgatagtgt taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag
     3181 ataatggcta agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaactac
     3241 tataaccagc acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact
     3301 ataaatcatt atttttcaat gcgttagaga acaatctact ttataaaaat tgaataaggc
     3361 tatttcagat tacatcactg aattcctaag ataaattagt gaacacaacc atagatatgt
     3421 cgaatgtatt ttcctgtagc attaatgaca cataaatatg aatagccata atttctattg
     3481 cttaagcagg atgcaagaaa ccaatttttt catagaggtt aactaatgga cttagaaaat
     3541 aaattctcat atcattttct tgagggatta acgctcacgg aagatggaat tcttactcaa
     3601 ggaaatgagc aagtttatat tccacagaaa gagttaggtg tattaatagt attacttgaa
     3661 tccgctggtc atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt
     3721 gttagtgacg agtccctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt
     3781 ggctatgatc gttgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt
     3841 ttcaaaacta aaattaatga agataatact tcagactatt ccatagctat attccctttc
     3901 actacttcat tgaatacact ggatccatta atacttaatc aggaattagt gcaaatcatt
     3961 tcaaataaaa aaatcgatg
//
LOCUS       DAJTVE010000239         3956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000239 DAJTVE010000000
VERSION     DAJTVE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..991
                     /locus_tag="OO439_004125"
                     /pseudo
     CDS             1..991
                     /locus_tag="OO439_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1007..1756
                     /locus_tag="OO439_004126"
     CDS             1007..1756
                     /locus_tag="OO439_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6318332.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1758..2822
                     /locus_tag="OO439_004127"
     CDS             1758..2822
                     /locus_tag="OO439_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318333.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(2842..3873)
                     /locus_tag="OO439_004128"
     CDS             complement(2842..3873)
                     /locus_tag="OO439_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6318334.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT         1123 a          872 c          873 g         1088 t
ORIGIN      
        1 taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc agcagtaatg
       61 tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa aacgtctccg
      121 cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa ctgtacctaa
      181 atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa cgaaatcagg
      241 ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat aattcctgga
      301 gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca
      361 gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca cagcgagaca
      421 gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt cacggagccg
      481 ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca gatggtgcag
      541 aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc ggagttattc
      601 catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat cttccagaaa
      661 atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct attggctacg
      721 ccaaacttga taccccaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
      781 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
      841 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
      901 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
      961 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     1021 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     1081 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     1141 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     1201 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     1261 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     1321 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     1381 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     1441 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     1501 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     1561 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     1621 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     1681 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     1741 caaacgagat aaataatatg cgctacttat taattgtaat tatatttatt ataggtttta
     1801 gtgcattacc tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag
     1861 tcattggtta cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct
     1921 ggcaaagcag tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac
     1981 ctggagaaga atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg
     2041 acttttataa tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt
     2101 cgactgcccc taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct
     2161 ttaataatca ctataaaaac cccgctgtct gtagtggagg tcctgagcct caattatcag
     2221 taacctttcc agctcgcgta caactctata ttaaattagc aaaaaatgcc aatagagtta
     2281 ataaaaacct ggtgttacct gatgaatata tcgctctaga gtttaaaggt atgagtggcg
     2341 caggagctat agaagtagac aaaaatttga ccttcaggat tcgtggatta aataatattc
     2401 atgtccttga ctgctttgtt aatgtcgcac tggaaccagc tgatggcgtt gttgactttg
     2461 gtaaaataaa ttcccgaaca attaaaaata ccagcgttag tgagacgttt agtgtagtca
     2521 tgacaaaaga tcctggtgcg gcctgtactg agcaattcaa tattttaggg agctttttca
     2581 ctacggatat tttgagtgat tatagccatc tggatatggg taatggactg ctattgaaga
     2641 tatttcataa agatggaaca gcaacggaat ttaaccgctt ctcacaattt gcttcttttt
     2701 catcgtctag tgcaccttcg gtcaccgcac cattcagggc agaacttagt gcgaacccgg
     2761 cagagactgt tgttgaggga ccgtttagta aagacgtaat cctcaaaatc acctataact
     2821 aaatagctgc gcttaatacc gctacacttt tgccagccca tgtttgcctc cggggaatgg
     2881 gctgacggtt tccataaaat ggcgaacttt tttcaacagt tgccacattg agctgcactg
     2941 atgattacgc gttattgtgt cgtgaagtgc ctgccatagc cgttcaacat gattcaccca
     3001 tggcgagtaa accggctgat aaatgaccct gaacttcggg ttctccttca gccagctctg
     3061 tgtttcccgg cttttgtgga taatgtagtt gtccacgatc agcgtgatgg ttttcgcccg
     3121 acggtatgtc gctttaagcc gcttcagcag gctgatgaac agcgccgaac ttttgctgtt
     3181 gccgcccaca cagctgactt tacctgtccc gctgtgcagc gctccggcca gataatattt
     3241 ttcattctgt cccggcgtga ccacccgttt ttgctgtccg cgcagttgcc agtccgcacc
     3301 gattttggga ttaagatgga tatccacttc atcttcataa aagaccggat gctctgcgct
     3361 gcattcgtcc agtgctttat ggattgctgc catcttttca tctttatgcg ggtcacggat
     3421 acgcagagtt ggcgcagccc ttcgccacac aatccccgca gacggcaacc agcggcgaac
     3481 ggttccggca tttaactggc aaccggttat ctcattgatt tttattgcca gcagttctgt
     3541 actccagcgt gaacgctggt agccaaagtc gccgggagaa tgttttacca gctcacgtaa
     3601 cagtgtgcag atatgctcaa acggccagcg acgggcacgc ccggcaggta atgatttcag
     3661 tccctcaaca cccgactgcg tgaaccagtt aatccagcgt ccaacagagg aacgggcgca
     3721 gcagagcgtt ctggcaacgt cgctgacacg gtcgccccgg tgcagcatca gcatggcagt
     3781 cagtctgcgg gcataatttt tatcgtgtgt tttatggatg gctttctgca tcaggcgtcg
     3841 ttcgtcacgg gaaattggtg ctatgatcgg cattgctcag tccggttggt gatttgtttt
     3901 gatttggcga ttgatcagat cgcacaatcc gggctaagtt ccctcaaagt gatcta
//
LOCUS       DAJTVE010000240         3951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000240 DAJTVE010000000
VERSION     DAJTVE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO439_004129"
     CDS             complement(<1..242)
                     /locus_tag="OO439_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6318335.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO439_004130"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO439_004130"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6318336.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO439_004131"
     CDS             2255..3904
                     /locus_tag="OO439_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6318337.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1030 a          999 c          925 g          997 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc c
//
LOCUS       DAJTVE010000241         3917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000241 DAJTVE010000000
VERSION     DAJTVE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO439_004132"
     CDS             <1..302
                     /locus_tag="OO439_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318338.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..2259
                     /locus_tag="OO439_004133"
     CDS             391..2259
                     /locus_tag="OO439_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6318339.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2296..>3917
                     /locus_tag="OO439_004134"
     CDS             2296..>3917
                     /locus_tag="OO439_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6318340.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1231 a          722 c          766 g         1198 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta tgttaataaa actgaccact
     1381 aatattggtc tggccgatga tgctgagtgg ctattctata atgctagttt tggcatgcag
     1441 catcccatac ttcacgcgat aatgtactgt aagactgata attgttatga agcaaatgaa
     1501 aggaaacatc ctgacagtaa tcagtttaat accagatata aatactcccc tactcatgag
     1561 gaggctgctg tctgctgcgt accgaataca gcaagaacca taccggcttt tgtcgaaaat
     1621 atgtttcaag aaaataacaa tggttttact gcattatttt atggcccgtg tgagttttat
     1681 ggcacattta ataatgttgc aattaaaatt acacaattaa ctgaataccc tcatgattta
     1741 agcgtaaaat gtctcattga gcctgagtca tctgttcgtt ttgctctcag tttccgttac
     1801 cccggctggg caaaaatgat gattattaat ggtgttactt ttactaaaaa tgatactcaa
     1861 aactcactta ttattttaga tagaacatgg caacatcatg atgtgattga cattaaattt
     1921 atagctgata tacagtttaa tactgactta tgtggtgaca cttatatttc cagaggtccc
     1981 gttttatatg caattgagct tgagagcgat attttaatca aaaaaaacct gctgaacggg
     2041 aaatattttg actcaggata cataccctgt agtagagctg atgaatctat ccagttctct
     2101 tatgctgata tggaatcttt ttcatattca acctcaccag agggcaaaca atacattgaa
     2161 ggctgttttt atttaaacaa gactaaaata gtcagaaaac ttataccatt tggtcaaacc
     2221 attcttcgca aagtaacttt ctccaatata gagagttaat attttcgagt gttataatta
     2281 attaaaggga tttctatgta ccttactgat aatattatca aactgtcgaa taaaataggt
     2341 cagcgtgttt atactgttgt ctctgaaatg tcgattgaag catggataac aaaagaaccg
     2401 cagccttttg tttatcgccg tagcggcgct taccagaaac tcaccattgg ctcagactgg
     2461 ggtcaactgt ttgactgtgc ctggatgcga gtttatggta aacgtccagc agataaattt
     2521 agacacaaac tcgtcgctct cgttgatatc ggtggcgaag gtcttattgt ggataagaat
     2581 ggttcaccca tctgcggcat cacgaataaa gcatcatctt atggcgtacc gccagataaa
     2641 ccaggtaaat gggtggttga cctttctctc gttagtgaaa atgacgaggt tgagttttgg
     2701 attgatgctg cctgcaacga tttatttggc tatgttacta atggagggat tattactgat
     2761 gttcatatcg cgacacgtaa tcaactactg aaatcccttt attatgatat tgaggtgctt
     2821 ttcgactgga ttaatgatgg tcagaagttt gaatccatac atcctaaagg tatcacccca
     2881 gaacaattta ttgccaaaag aagtgagcgt actgacgaaa tcataagtac tctcgaatat
     2941 attgataata cgttaattac ctttagcaat gaagaaataa ttaaatgtca aattgcaatc
     3001 caatccataa tcaataaaaa caataatcct tcagagttca ggattatggc tactggtcat
     3061 gcccatttag atatagcgtg gatgtggcct ttaagagaag gacgcagaaa agcaataaga
     3121 acatttgcca ccgcccttgc aaacattgaa aaatatcctg attacatttt tggcgctagt
     3181 cagtaccaac tttttcactg gataaaaaaa gattatccct atttcttcga aaagttaaaa
     3241 aaacaaatcg ccgcaggccg ctttgagtta caaggttgct tctgggtcga atgcgattta
     3301 aatctggtaa gtggtgaatc gctgataagg caaatcatgc atgggacacg attcacactg
     3361 cagaattttt caaaaaagat aaattatgtc tggcaaccgg atgtctttgg attccccgca
     3421 acgcttccac aaattctcaa aaaatcaggc attaactata ttgcatcgca aaagctctca
     3481 caaaacaaga ttaataaatt taataattac cttttccgct ggcagggcct ggatggctca
     3541 gaaattctga tgcataactt tcctgaagac acttatgata gccgagcacg tgctcgctct
     3601 ttagaatata ttgaacaaaa ctataatgaa aaagagatct gtccctatgc gcttatggtg
     3661 tatggtgtcg gtgatggcgg cgctggcccc ggtgaggaac atatagaacg attaacacgt
     3721 atacgcaata ttgatggctt acctcatgtt gatttcagcc gtgttgataa gttcttcact
     3781 tacgccgacg cctttcgcga aagcttgccc attatttcag gtgaacttta tttcgaggct
     3841 catcaagggt gctttacatc agagtcagca actaaagctc ataatcgaaa tatggaaaat
     3901 aaattacatg atgctga
//
LOCUS       DAJTVE010000242         3908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000242 DAJTVE010000000
VERSION     DAJTVE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..590
                     /locus_tag="OO439_004135"
     CDS             24..590
                     /locus_tag="OO439_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6318341.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="OO439_004136"
     CDS             594..1169
                     /locus_tag="OO439_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6318342.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="OO439_004137"
     CDS             complement(1211..2263)
                     /locus_tag="OO439_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6318343.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="OO439_004138"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="OO439_004138"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6318344.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="OO439_004139"
     CDS             3580..3777
                     /locus_tag="OO439_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6318345.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAJTVE010000243         3811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000243 DAJTVE010000000
VERSION     DAJTVE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..255)
                     /locus_tag="OO439_004140"
     CDS             complement(<1..255)
                     /locus_tag="OO439_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6318346.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLD"
     gene            342..749
                     /gene="nudG"
                     /locus_tag="OO439_004141"
     CDS             342..749
                     /gene="nudG"
                     /locus_tag="OO439_004141"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6318347.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(715..987)
                     /locus_tag="OO439_004142"
     CDS             complement(715..987)
                     /locus_tag="OO439_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6318348.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1223..2566
                     /gene="gdhA"
                     /locus_tag="OO439_004143"
     CDS             1223..2566
                     /gene="gdhA"
                     /locus_tag="OO439_004143"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6318349.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2683..3722)
                     /locus_tag="OO439_004144"
                     /pseudo
     CDS             complement(2683..3722)
                     /locus_tag="OO439_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          987 a          930 c          913 g          981 t
ORIGIN      
        1 atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac cgtccagacc
       61 atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc ccagcgccag
      121 aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat ctggcgtaat
      181 gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc ggggatgaag
      241 atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag caacagtgcg
      301 gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg
      361 ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg
      421 atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa agccagcagc
      481 aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg ggtgagtatg
      541 ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc tggcacgtac
      601 ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg tgctcacctg
      661 aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag gcgtttatgg
      721 ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc gctggcattg
      781 cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat cctgataaac
      841 gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt ctggcggtac
      901 gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg gaaatgctaa
      961 tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt ctctgcaact
     1021 gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg gttatgagat
     1081 tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg tcacattctt
     1141 gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca caatcgtatt
     1201 aatatataag ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca
     1261 tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac
     1321 cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg
     1381 tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaacca
     1441 gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc cgtacaaagg
     1501 cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg gctttgaaca
     1561 aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg gcagcgattt
     1621 cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc tgatgaccga
     1681 gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg gcgttggtgg
     1741 tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt
     1801 cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag ctaccggcta
     1861 cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt ttgaagggat
     1921 gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga
     1981 atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg
     2041 attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg atggtcgagt
     2101 ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt
     2161 accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg acgccgcgca
     2221 tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc cgaccaccat
     2281 cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta aagcggctaa
     2341 tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc gcctgggctg
     2401 gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg
     2461 tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg
     2521 ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg
     2581 atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg
     2641 taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga
     2701 tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca
     2761 aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact ttctgcgata
     2821 gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat cttcttcgct
     2881 ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc
     2941 ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg ataattccgc
     3001 cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat cagagatata
     3061 gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc
     3121 catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaggca
     3181 caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg tcttttactg
     3241 atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt accaggttca
     3301 ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa tcgataaggt
     3361 gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc tttagcctga
     3421 taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcata
     3481 attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt caaaccgttg
     3541 gatgttatcg ctgcgaacac aaacattaca gcgacaacac atgttaaata aaacccccac
     3601 ttacaaagca gcatggcctt attttcttta atcatccgtt caaaattact attaaatatt
     3661 tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa aacttttttc
     3721 atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaaata
     3781 tccaacgaat tttcttgatc cgctggatat t
//
LOCUS       DAJTVE010000244         3724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000244 DAJTVE010000000
VERSION     DAJTVE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..181)
                     /locus_tag="OO439_004145"
     CDS             complement(<1..181)
                     /locus_tag="OO439_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR6318350.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OO439_004146"
     CDS             425..682
                     /locus_tag="OO439_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6318351.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO439_004147"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO439_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6318352.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO439_004148"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO439_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6318353.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO439_004149"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO439_004149"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6318354.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3724)
                     /locus_tag="OO439_004150"
     CDS             complement(3307..>3724)
                     /locus_tag="OO439_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6318355.1"
                     /translation="VGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAP
                     IFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYS
                     LVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT          999 a          885 c          890 g          950 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacg
//
LOCUS       DAJTVE010000245         3694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000245 DAJTVE010000000
VERSION     DAJTVE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="OO439_004151"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="OO439_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6318356.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OO439_004152"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OO439_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6318357.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OO439_004153"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OO439_004153"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6318358.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..>3694)
                     /gene="caiC"
                     /locus_tag="OO439_004154"
     CDS             complement(2210..>3694)
                     /gene="caiC"
                     /locus_tag="OO439_004154"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6318359.1"
                     /translation="TALICESSGGVVNRYSYLELNQEINRTTNLFYTLGIRKGDKVAL
                     HLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQACLLVTSAQFYPMYQQ
                     IQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQPATLCYAPPLSTDDTAEILFTSG
                     TTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAGA
                     TFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTLMAQPPSANDRQHRLREVMFYLNL
                     SEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPGDKRRWPSIGRAGFCYEAEIRDDH
                     NRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAKVLEADGWLHTGDTGYCDEEGFFY
                     FVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGE
                     TLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIRKNLK"
BASE COUNT          878 a         1004 c          905 g          907 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccgaaccag caaaagataa
     3541 attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat
     3601 aaaacaggtt tgtcgtgcgg ttaatctcct gatttaactc aagataacta taccggttaa
     3661 cgactccgcc gctggattca caaatcagcg ccgt
//
LOCUS       DAJTVE010000246         3665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000246 DAJTVE010000000
VERSION     DAJTVE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..117
                     /locus_tag="OO439_004155"
     CDS             10..117
                     /locus_tag="OO439_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6318360.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(204..1883)
                     /gene="bcsG"
                     /locus_tag="OO439_004156"
     CDS             complement(204..1883)
                     /gene="bcsG"
                     /locus_tag="OO439_004156"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6318361.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1880..2071)
                     /gene="bcsF"
                     /locus_tag="OO439_004157"
     CDS             complement(1880..2071)
                     /gene="bcsF"
                     /locus_tag="OO439_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6318362.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2068..3639)
                     /gene="bcsE"
                     /locus_tag="OO439_004158"
     CDS             complement(2068..3639)
                     /gene="bcsE"
                     /locus_tag="OO439_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6318363.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          929 a          920 c          940 g          876 t
ORIGIN      
        1 ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg
       61 gtcattgctg gcattcttgc cagtatgatc gtgaactggc tgaataagcg gaagtaacgt
      121 gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcagaggtt
      181 aaactctgcg gcctttttcg tgcttactgc gggtaaggca cccagtcgcc gccgttcagg
      241 cgaacgtacg gtttatcctg gtattgaata actactgcat ttgagttctc ggagaccggt
      301 gctgtttgtg gcaacccact ggtgagtttt ttccagtcaa cattgtcttc ggtgaaaatc
      361 ttgccatcaa gaacgcgaac caccagatcg gagatagcta ggaagctgct cggttggtca
      421 atgacaatcg gtgccccctg atgtggtgcc ttcatgccga agaatttcac cccaacgggg
      481 acgtctgtga tagacgggct agggatatca cgtaggccag atacctgcat tctgtcgccc
      541 ttcagcgcgc cgccgtgttc cggcaccacg accaccatca ctttacgacc cgatttctcc
      601 agttcagtaa agaaggcgtc cagttcatca aagaatttct gcgcccgcgc tttgtaatcc
      661 gctgttttgc tgacccccgg ataatggttg ccgtcatgca gtggaagcgt gttgtagaac
      721 gtggcactac ggctgttttt atctttttcg gtaacgtcca gccagcggtt aagcacggcg
      781 gtatcgtcat aaaccggcga accatcaaag cccagcaaaa taaccggcag atttgtttga
      841 tccatcaatt cagtctgcat gccgccattt tcgcgaactt ctttcaaaaa accgccgaac
      901 tggccgttat gtcccatcat caggtgctgg gtaaagccca gtttcgaaag gttatcaaac
      961 agatagcagt cgttatttgc cggttgatac agattagtgt gcgaagtctg cccgcagctg
     1021 gcgcgcagta aacggatcgc cgccgggcca ctgtaggagg tggcggagtt aaagttcttg
     1081 aactcaatat cgaaatgcga ccacagtgga tgcgacatca acccggcggc ttctatatcc
     1141 gaccaggaaa gcgaacagat gttaatcacc agtagttcaa atggctgagc atcagcgggc
     1201 agcgaagacg ggaaggtcga tttacgtttc gcctccgcgt tatagaaatt attcagccag
     1261 gcgttaaggt tcgccgttgt tggcggtgca gtttgtgcgg gcatatcacc cactaccgat
     1321 gcgccacccg tcgccgcaac ggttgccgct gcgttaccac ccgtcgttgt tacagtggtc
     1381 gtcggttgtc cggctggcca caaggagaaa cttggtcccg ccagggtaag tacgttcagc
     1441 catagcagta tggcaaccac aaaaacggta atgcgaatcc attgtgacag gaataaccag
     1501 gccactaata aaacaaaaat ggccccaatc atctgccagt taataaagcg tgtgacaagg
     1561 tcgattaaat aatcggtact gaaccccgcc acctgcgaac cctggctcat tatgctttcc
     1621 gggccaggca accaggtgtc atgccagaac aaagcaaagc cgatcggcag ggcaatccag
     1681 tggcgcaagc gatgcaggct gtagcgcgga atgggcatca gcagaaacgt ggcaaacacc
     1741 aaattgagga gcggatggaa gttaagatat cccgcccaca acaggccgaa cttaaccaga
     1801 aaatagaagt tccagccgga aaggccgcgc cagtattgcc agagggaaga aggcatggcg
     1861 gtattttgcg taaattgagt cattttttgg ttgccctggc tttttccgtg cggcgtaacg
     1921 tcccggccgg tttaacataa cgaggtttag caaagaataa acgtaaggtg tcgcgaatgc
     1981 gtcgcaaaaa gtgccgcgcc agatagccca gcgggaaaaa tatcagtgcg caaacgacaa
     2041 taatttcaat gatatcgctg atggtcatca tgatgagcgc tccacagcat catctaacag
     2101 tcgcatgggt tctggtattc gtcgccagtg gcgaccatcg tgctcggcat tgatgaccgg
     2161 tttagaactt tgcgttaaag gcagcggcat gccccattgt tctggggcaa gcaagcgcat
     2221 ctgcaccagc tcggcactga tttgatcatc ttcaaaccag accatacggt ttgagaaaat
     2281 gtcgccagta ggcaatggga aaatatgatt caacgcggta tccagatcgt taatccgaca
     2341 gaatgagaga aacagcacca gccgattacc gccaatggtc atgatatcgc cggtacggtt
     2401 agggcgacac agcgtcaggg cttgttcaac gcggataccc ggtaccggac gtagggcaac
     2461 cagaacgcct ttaccgtggg caggtaatag agggttattc atcatgttgt tgacggcatt
     2521 acagaacaca tcccacttct ggaaaccacg cagtttgagc ggctgggtca ttgacagcaa
     2581 ggtagtgata tcttccggca catagcgact aaacttctgc ccttgcacgc tttcgatcat
     2641 cgtcagacaa cgggagagtg gcgcattcca cggaataacc atatttgcac cgcaggccaa
     2701 taacaaacgt tcatcggtgg cgcgcaggct agcggtattt tcccgcacga ggattttcat
     2761 cgcactaccg cgctggcgac gcagggtatg aatgctgcgg gccagtggct cgatttgcgc
     2821 attttgctgt aaagaaaaga ccaccgtcgc cgcctgagcg gtacgggctt cattgaacag
     2881 gacttcgtta ttgttgaaca gttgccagtg ttccgatagc ggcggcgcac cttccagtac
     2941 agcaacatta ctcagaatgc gtttttcgtc gctgcgtggt tggatctccg cctcttcgct
     3001 ttgaactaat gtccagatac cattttgttg ctgaacgcta agctgctgac gggcgctgac
     3061 ccctttttcg ttgcaccaga aggcaatatc cagcaaatgt tggtcaccct gaaaacgcaa
     3121 actggcaaga ccaaaaagtg aacggtattc ctcaagcaac aatgaaaatt gtttatcgtt
     3181 attatttccg ggattaatta ccaaaagcga acaatggttt aacctgctcc atttattcat
     3241 tttatccaac catgagcgaa gccgctcggc aggaatgttt tgccatgcgt tatttgcgca
     3301 aacaagaata aaaaggtaat tatggggatc aatagaacac tgcaaatcac gggccaaata
     3361 gtatagacct ttttcatgat ttagcattga aaataatttt attttttccg gcccttgaga
     3421 atcatctaat tgaaagattt tcgccggatc gctgtccatg ctaatgaccg cgacgtgtgc
     3481 ggtttcagcc tgggatgcaa ttgtttgatt cgccagactg atagcatctt catggcgatc
     3541 gacgttaaac caccagacgc cgcctgctgg catatgtcgc agctcatccc ataatgatga
     3601 gataccgata gagaatacag ggtccacaat gtccctcatg tcaccatttt gtctgattat
     3661 cagtt
//
LOCUS       DAJTVE010000247         3664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000247 DAJTVE010000000
VERSION     DAJTVE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1317
                     /locus_tag="OO439_004159"
     CDS             <1..1317
                     /locus_tag="OO439_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6318364.1"
                     /translation="IYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAVHLMLGVAASR
                     VKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDRAVDDHRHQF
                     WALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFYQRLAQDLG
                     LTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYLQHS
                     IVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVHKYQ
                     GIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVRQLL
                     RIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQIRR
                     ISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            1318..2934
                     /locus_tag="OO439_004160"
     CDS             1318..2934
                     /locus_tag="OO439_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6318365.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(2990..3142)
                     /locus_tag="OO439_004161"
                     /pseudo
     CDS             complement(2990..3142)
                     /locus_tag="OO439_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3165..3542)
                     /locus_tag="OO439_004162"
     CDS             complement(3165..3542)
                     /locus_tag="OO439_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318366.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         1056 a          708 c          918 g          982 t
ORIGIN      
        1 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
       61 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
      121 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
      181 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
      241 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
      301 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
      361 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
      421 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
      481 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
      541 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
      601 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
      661 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
      721 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
      781 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
      841 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
      901 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
      961 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
     1021 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
     1081 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
     1141 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
     1201 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
     1261 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
     1321 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
     1381 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
     1441 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
     1501 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
     1561 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
     1621 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
     1681 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
     1741 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
     1801 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagca gtcggccgta
     1861 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
     1921 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
     1981 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
     2041 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
     2101 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
     2161 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
     2221 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
     2281 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
     2341 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
     2401 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
     2461 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
     2521 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
     2581 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
     2641 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
     2701 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
     2761 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
     2821 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
     2881 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
     2941 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
     3001 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
     3061 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
     3121 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
     3181 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
     3241 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
     3301 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
     3361 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
     3421 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
     3481 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
     3541 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
     3601 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
     3661 gata
//
LOCUS       DAJTVE010000248         3662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000248 DAJTVE010000000
VERSION     DAJTVE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..385
                     /gene="mpaA"
                     /locus_tag="OO439_004163"
     CDS             <1..385
                     /gene="mpaA"
                     /locus_tag="OO439_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6318367.1"
                     /translation="NSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDP
                     LACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSD
                     EASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(360..1325)
                     /gene="ycjG"
                     /locus_tag="OO439_004164"
     CDS             complement(360..1325)
                     /gene="ycjG"
                     /locus_tag="OO439_004164"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6318368.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1444..1950
                     /gene="tpx"
                     /locus_tag="OO439_004165"
     CDS             1444..1950
                     /gene="tpx"
                     /locus_tag="OO439_004165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6318369.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(1994..3535)
                     /gene="tyrR"
                     /locus_tag="OO439_004166"
     CDS             complement(1994..3535)
                     /gene="tyrR"
                     /locus_tag="OO439_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6318370.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          892 a         1035 c          862 g          873 t
ORIGIN      
        1 gaacagcgcc gctgaagaac gtgatgtcgt attactgaca ggtgacaaac ctggctcaga
       61 acctgaaacc caggcgctgt gccagcttat acaccgcatt caacctgcct gggtggtctc
      121 attccacgat ccactggcct gtattgaaga tcccagacac agcgaattag gcgaatggct
      181 ggcccaggcg tttgaattgc ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt
      241 cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc gcagagtttc cgcccatctc
      301 ctccgacgaa gccagcgaaa aatatctctt tgcgatggca aacttgctgc gctggcatcc
      361 taaagatgca attcgcccgt cgtgaactga agcgccggtt ccacatctac cgccagccag
      421 gtcggtccgt caagatcggc gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg
      481 gcacgagagg tacacaacat gcagcccagc atcagactga aaccttgtgc acgcgcttca
      541 gtcgccagcg ccagcgcttc cgtcagacca ccgattttat cgagcttaat gttaaccatc
      601 tcatagcgcc ctttcagcgc cttcagattg ctacgtgtat gacaactctc atcagcacaa
      661 atcggcaacg gatgaataaa attctccagc gccgcatcat cctgcgcagg aaggggttgt
      721 tcaagcatcg caacgcctaa atccgccaat agctggcaac gcgccgccac cccttctgca
      781 cgccaggatt catttgcatc aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc
      841 accatccgct cactaataag atggttatcc agcttcactt tcagtaattt cgcgcctgcc
      901 tgccagagtg ttgatgcact attggccatc tgatcaggcg taccgatgac taccgtctgt
      961 gcagtgataa ctgtctcggg aagcgttatg ccgatcaaat cagccagcga ttgctgctgt
     1021 tttcgcgcgg ccagatccca caatgcacaa tccagcgcat tacgtgctgc gccggcaggg
     1081 agaatttttt gcaactcctc ccgtgtcagc cctttctcta attgcgacac gacgctcata
     1141 atttgcgcca ttaccgaggc atcactttct ccataacgcg gatacggcgt gcattcgccg
     1201 gtgcctttaa taccctcttc ttccagttca accaccacca cgcgcgcttc actgcgactt
     1261 ccccgggcaa tcacaaacgg ggtatgtaag ggccaggctt cctcaaatac cttaacggtt
     1321 ctcatcttta ctccttgttg tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg
     1381 tgttattgac agttagcata aactaggtgt gacgttaact atatgtaaac aggaaagata
     1441 atcatgtcac aaaccgttca tttccagggc aacccggtta cagtcgccaa ttccatcccg
     1501 caggcgggta gcaaagcgca gacttttact ctcgtggcaa aagatctgtc tgacgtcacc
     1561 ctcggtcagt ttgcgggtaa acgcaaagtg ctgaacattt tcccgagtat tgataccgat
     1621 gtttgcgccg catcagtacg caagtttaac caactggcaa ctgagatcga caacaccgtt
     1681 gtgctgtgta tctctgccga tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt
     1741 ctgaacaacg ttatcaccct ctccactttc cgtaacgctg aatttctgca agcttacggt
     1801 gtggcaattg ctgatggccc actgaaaggt ctggcagcgc gtgccgttgt ggttattgac
     1861 gaaaatgaca atgtgatttt cagccagctg gtggatgaaa tcaccaccga gccggattac
     1921 gaagcagctc tggctgtact gaaagcataa gttaatatgc ctgatggtgt tgcaccatca
     1981 ggcatattcg cgcttactct tcgttcttct tctgactcag accatattcc cgcaacttat
     2041 tggcaatcgc ggtatgtgaa acgccgagac gttttgccag tttgcgcgtg ctgggataat
     2101 tgcgataaag ctgggttaat accgagcgtt caaaacggct ggtgatttcg tccagcgaac
     2161 cttccatcgc atcttcgccc acggctaccg ttgcggcgtc ataatccggc aacaaaatat
     2221 cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc gcgatagata gcgttcttta
     2281 actgccgcac atttcccggc cacgcataac gcgtaagtac agtattcagg tcagcggcca
     2341 gtttcggacg ctgcacgccc tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg
     2401 gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt gagcgtcaac acgttcagac
     2461 gataatagag atcttcacgg aacatgcctt tttgcaccag ttcgaccaga ttcttctgcg
     2521 taacgcaaat cacccgcaca tcgacatgca cctcatggtc ttcgccaacc cgacggaaag
     2581 tgccatcatt aaggaaacgc agtaatttcg cctgcatccg tggtgacatt tcccctattt
     2641 catccaacag caccgaacca ccgttcgcct gctcaaagaa tcctttcttc ccttccggag
     2701 catgaccaaa cagttcactc tcgaccgcat cttccggtat agacgcacag ttcagcgcca
     2761 ggtaaggttt gcccgctctg gggcttgcct gatggcaggc gtaggcaaag agatctttac
     2821 ctgtacctgt gtcacccgta atcagcagcg gcgcgcttag catcgccagt ttctgcgcct
     2881 gttcgacaac atgcttcatt ttcgggctga cggcgacaat ttgactgaag gcgctgacgt
     2941 cctgggcggc gacattttgc aactggcggc ccatacgaat cgttgatcgc aacatcacca
     3001 ccgcaccggt caggacgtgt tgatcatttt catcctgaag ataaacaggc gtaatctcca
     3061 tcaggaaatt ctgcccatta ataacgacat gctcgttatg cgaatcttgc ggttcgcttt
     3121 ccagccaacg taaaaaatta aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc
     3181 gatccaattt ttgcccaaaa agctgacagc tcgccgggtt cgccatatcc actttgcttt
     3241 tcatatcgac agagagcaca ggttcaggca acgcctccag taacgcgctc aacgccagat
     3301 gctcacgttc ggaaggcatc cacgggacag tacgcacatc ggtaacaccc gcaatacggc
     3361 gtatttcggc catcagactg ctgaaactct caaactccag ttcagcaaaa ttgaggtaga
     3421 ttcgcccaat gggatcaatc tcaataccgc gtaaatcaat gcctcttagc acgagtagat
     3481 cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa
     3541 ccttcacctg aaaaaagaac aattaatatg atgatatgac actatttgga tagcaggaag
     3601 gtttctgtca acaatcattg acacaaagac ggaaaaacgg gcacttcggc gtaaagatat
     3661 cc
//
LOCUS       DAJTVE010000249         3567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000249 DAJTVE010000000
VERSION     DAJTVE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            753..1469
                     /gene="nanC"
                     /locus_tag="OO439_004167"
     CDS             753..1469
                     /gene="nanC"
                     /locus_tag="OO439_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6318371.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1489..2595
                     /gene="nanM"
                     /locus_tag="OO439_004168"
     CDS             1489..2595
                     /gene="nanM"
                     /locus_tag="OO439_004168"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6318372.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2660..>3567
                     /locus_tag="OO439_004169"
     CDS             2660..>3567
                     /locus_tag="OO439_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6318373.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHF"
BASE COUNT         1079 a          740 c          802 g          946 t
ORIGIN      
        1 cgacttcaga agcgaccagt tacaagccac aaatcaggaa acatatttca tggtgagaat
       61 tatgatcagc ctgatggcgg gtaaacactt gttttcacca cgctcactga gttagcttgc
      121 aaagcttccc ttgtttagac cctaaataaa ttttttataa aacaaacagt taaaagaaat
      181 cctttagttt ttgaagacca cctcaagtgt tcgtctggct tcacatggca tcttcctctt
      241 agaaaaagat cgacatattt tgtgacacga attgcaaatc tcgttttgtt gtatggcttg
      301 cgtgattttt gatctggtat aacaggtata aaggtgcacc gcgatagtca atgagacagg
      361 gcatctcgca atctatggca aacatcactt cagttctttc tcatcgggtg atgaaaacgc
      421 acttcagtct gaaaggaata tgaaaatgag atcaacagac actctatttt atgactctgg
      481 gtaaaatgga ttgagtaagt gatatagctt acgaacattc aaatcaatta aacatcagaa
      541 gagattttat actcaggtat ttaatctgat tctctgttta tttaaataat gtggaaagag
      601 aattttcaca ggagacctta tacaaaaata taaaataagc tacccgttgc caaagacact
      661 ataagcctgg caaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat
      721 tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg
      781 tattattact gtgcttttcg tcaccattaa tttctcaggc tgcgacactg gacgtacgtg
      841 gtggatatcg tagcggaagc catgcctatg agactcgact caaagtcagt gagggatggc
      901 aaaatggatg gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa
      961 aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg
     1021 atcaatggac ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg
     1081 gaccctacgt aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc
     1141 gttacgactg gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg
     1201 ttcatcgttg ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc
     1261 aaacgacgct atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga
     1321 cggaaaatgc ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat
     1381 atgactacct tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt
     1441 atcgcattgg tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact
     1501 ataatggcgc ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa
     1561 actcctgtgc catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt
     1621 ttaggtagcg caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg
     1681 actgcgttag ctgcattccc tggtggaccg agagatcaag caacctctgc atttattgat
     1741 ggcaatctat atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt
     1801 aatgacgtac acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgctcgcg
     1861 ccgatgggca tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc
     1921 ggtgttaacc agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat
     1981 tcaaccgcta tagataaaat caacgcccac tattttgaca aaaaagcaga agattatttc
     2041 ttcaataagt ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa
     2101 tcgccctggt acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt
     2161 attaatggcg aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc
     2221 ggtaataatt taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc
     2281 ggttttgcgg ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt
     2341 tcacgagaaa attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat
     2401 agcactgata ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa
     2461 ggtcgggcct acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa
     2521 actgcaggcg gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc
     2581 acagtacaaa actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt
     2641 tgtattacgc gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac
     2701 cgttgctggt cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga
     2761 agatgcgcct cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg
     2821 cccgccatgt cactttaacg acattattcc actgactcac tgcccacacg atgttcagga
     2881 tatgcagggt tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg
     2941 ccaggcactg catattgcac ggaaattact gcccttcatt cctaataatg cagggattct
     3001 tatcgttccg tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc
     3061 agaacggcac ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca
     3121 ggatttagtc agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg
     3181 cgtatgctgg atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca
     3241 acactttaat catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct
     3301 taataatatt actgacgcgc catggttttg cggcgatacc acctggtact ggaaagaaaa
     3361 tttcccgcat tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat
     3421 tattttcgtc gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga
     3481 cccggacgat ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac
     3541 gacggcactg cgaagcagtc atttcag
//
LOCUS       DAJTVE010000250         3494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000250 DAJTVE010000000
VERSION     DAJTVE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..1366
                     /locus_tag="OO439_004170"
     CDS             119..1366
                     /locus_tag="OO439_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6318374.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1895..2095)
                     /gene="torI"
                     /locus_tag="OO439_004171"
     CDS             complement(1895..2095)
                     /gene="torI"
                     /locus_tag="OO439_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6318375.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2218..2562)
                     /locus_tag="OO439_004172"
     CDS             complement(2218..2562)
                     /locus_tag="OO439_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318376.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2804..3406
                     /locus_tag="OO439_004173"
     CDS             2804..3406
                     /locus_tag="OO439_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318377.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          871 a          703 c          803 g         1117 t
ORIGIN      
        1 cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga gaagtaaacg
       61 gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt aggtaattat
      121 ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt actcatttct
      181 cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga aaggacatct
      241 agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta ccagcattct
      301 atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac cgctcatctg
      361 gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt atgaaccgtt
      421 actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg gcctggggta
      481 tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa attttcattt
      541 cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg cgttctttgc
      601 cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc tatttggcgc
      661 tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg tagcggcgga
      721 tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa acttctgggt
      781 tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc aacaactttt
      841 tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc tgtatggtta
      901 tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc ctttctttgt
      961 gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg cgttgcgtat
     1021 cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc tgttacatgc
     1081 cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa attttgataa
     1141 gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc ttgggattgt
     1201 gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga cagttttctt
     1261 cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc tgagtaaaaa
     1321 acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt aaacttttcc
     1381 ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa agtgcttagc
     1441 ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt cgctcaaatg
     1501 tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc gacctgtccc
     1561 gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt atcgagtcac
     1621 gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca gggctgttta
     1681 gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa atgagtagat
     1741 ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt tcgtttgaag
     1801 ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat tggaatgctg
     1861 ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg cttaagagct
     1921 tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg ataactttgg
     1981 ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg aagccagtat
     2041 cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt tgcatgagtg
     2101 ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc attgatgcag
     2161 caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg aggcgggtta
     2221 gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc cgtacgtctt
     2281 catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc ctttgaatgc
     2341 cggaacacgc tttgcaacct tgtcgaatga atctctgatt tctggaatct gctcaacaag
     2401 tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat gaccatagat
     2461 attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc cgacatcgcc
     2521 agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc tccataaaac
     2581 aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg atgctgttca
     2641 ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt tgggttggtc
     2701 agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc taaaatccat
     2761 gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct attttctgtg
     2821 caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc taaacatgaa
     2881 aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct ttattttcct
     2941 tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga gtatatccca
     3001 ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt gggttaagaa
     3061 ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg tttatgcagg
     3121 gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa cttatttttt
     3181 gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta gttcaaaaag
     3241 ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca tttgctatcc
     3301 gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta atacattagg
     3361 tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata gtatatagcg
     3421 ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt tattctggta
     3481 tgaatatttc gctt
//
LOCUS       DAJTVE010000251         3479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000251 DAJTVE010000000
VERSION     DAJTVE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            158..904
                     /locus_tag="OO439_004174"
     CDS             158..904
                     /locus_tag="OO439_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6318378.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            919..1341
                     /locus_tag="OO439_004175"
     CDS             919..1341
                     /locus_tag="OO439_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6318379.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1399..1755
                     /locus_tag="OO439_004176"
     CDS             1399..1755
                     /locus_tag="OO439_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318380.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            1848..2066
                     /locus_tag="OO439_004177"
     CDS             1848..2066
                     /locus_tag="OO439_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6318381.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2068..2433
                     /locus_tag="OO439_004178"
     CDS             2068..2433
                     /locus_tag="OO439_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6318382.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            2430..3095
                     /locus_tag="OO439_004179"
     CDS             2430..3095
                     /locus_tag="OO439_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318383.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3095..3460
                     /locus_tag="OO439_004180"
     CDS             3095..3460
                     /locus_tag="OO439_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6318384.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          941 a          747 c          974 g          817 t
ORIGIN      
        1 ttttacacct gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc
       61 gccgaaaggt gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga
      121 cagtgcgatc ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg
      181 cgttctggaa cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt
      241 agcggagtgg cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg
      301 cctgaatcgc cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca
      361 ccgtaaatgc tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag
      421 ccaggcgaaa tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg
      481 aaaacctggt accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga
      541 cggtcagaca gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta
      601 tgacgacggg cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt
      661 tcttgatgaa attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat
      721 tgttgatcgc cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga
      781 ggtgatgaaa acattgctcg gcgagcgggt gatggatcgc atggtcatga acggcgggcg
      841 ctgggtgaat tttaactggg agagctggcg tccgaatgtt agccattcga gggttgttaa
      901 gtagtttcag gaggatttat ggcgaaacct tttactcccg aacagcggga agaactgaag
      961 acgcgaattg tggaactcgt gcatcaggac ggtcgggtca cgattcggca gttgtcagat
     1021 gaaacaggta tcagtcgtgc gtctgtcggt cgcttatgca tagaactggt cgcaagtggt
     1081 gatgtatata attctggcta cggcttattc ccgtctgaac aggctcgcaa ggactggcaa
     1141 agcgcccgca aaaaactctc gagagtaaag gtgaggaaac cggttgttgt tgatccggac
     1201 cttatctggt cattacctga cggagaaata cgccgctacg acaggcgcct gaatataatc
     1261 tgtcgcgagt gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat
     1321 tttcaggagg cgatactgtg aatgaaatta gctatcaggc ttcaattacc gctggcattc
     1381 gcatcaaagg agaggagcat ggaaataaaa ccagaagatg agttaagcaa tatcgtttta
     1441 tttccggtaa aagaggatga ccctcgtaat caggttaatt ttctttatga gccatcggaa
     1501 agaccatatt gccatcacgc ctctgtacgg gttgacgaaa aagagcgtca ggtccgctgt
     1561 aaaatctgcg gtgcggttgt ggagccgttt gactggatgc tctctgtggc aaaaagagaa
     1621 accagactgg cagatgatgt aaggctattg cgccaggagg aacaggaaag gcggagaaat
     1681 atagaaaagc tgatacagat tgagcgtaac gcgaaagcgc ggatacgcag ggcgacaaaa
     1741 tccagaactg aataattaaa tttagcactg ttaaaaattt aatccttaac cggagggatt
     1801 tctgcaccct caaatcatca ggagaccgcc cgaaagggcg gtaatgaatg gttacattat
     1861 ttagaaaaaa atatccgcga aagagtagaa caacagaatt cctgtttctc attctgttta
     1921 tcgtgttgat gacaccgata tccccgctaa tttttgtctg gataatcgga aaaataattg
     1981 agccagttat tgaattgtat aacgacgtgg tatgggcgtc attcaacaca ctgcacaata
     2041 aaattaatcc gtataaggaa agctgatatg gcactgacga aaaaacaacg tgcagaactg
     2101 cgcatgaagt tcggtggtcg ctgcgcttat tgcggctgcg aacttggcga aaagtggcat
     2161 gcagaccatg taaaaccggt cattcgtttt gatggaaata tgcttcacca ggaacgtgac
     2221 gatatatcca acatggtacc agcatgccac ccatgcaatc tgcacaagca ttgcagtagc
     2281 ctggaagatt atcgacgaat tatcagtgat ggtcgtcgtg aattccttgc gtccgggaaa
     2341 ggcaaagcgc tggttcgtat gggattggtt gaaatgaaat ctgacccggt tgtgttctgg
     2401 tttgaaaaat atcaagaagg ggctacggca tgaccactat taccaaagag cgactgcaat
     2461 ggctggctaa catttctggc cgcgatgata ttgacgatat agacggcggt gaaattcgtg
     2521 aactggcgct tatcgctctg gcatcactgg aagcagagcc tgtagcggaa tgcattgttg
     2581 aagatggggg catgtgtatt gacgggttcg gtgagtatgt gggtcactcg ctgcctgatg
     2641 gaatgcatga gctttatgct gccccgcgaa tgcgacaacc tgatggatgg aaagccgtag
     2701 ctgtagcgtg gaaggtgacg tttactcaag ttgaccagga atctaatacg ttcactgcta
     2761 tatattttga caaagcggaa gttgagtgtt gggtacgact gcataaagca tgtgattttc
     2821 gggcagatat aacaccgctt tacgcagcgc cggcagtgcc ggttgcagta aacgacgaca
     2881 tggcttacgc attccatcac gcactgtcag attcatcgct tggctctgat gaaatcgaag
     2941 aaattaaaac cggtttgcgt gctgcctttg ccaacgtcac tatccaacca gagccgatag
     3001 tgccggatga aatcgggcca aacgatagta atacgtttga ttatgttgat ggctggaacg
     3061 cctgccgcgc tgccatgctt aagggagata aataatgatt aatcgaacca aactggagca
     3121 cattctcgaa tatgccaggc agcagaaatg tattgggcaa ctttgtaaaa ttccaccagg
     3181 agatatggtt gaaatcgtgg aaatggccat gcgtaaggct ggcaactctc cggtagctcc
     3241 ggctggctgg ataagctgta gtgatgcagt tcctgctgaa tattgcgatg tgattcttct
     3301 cgatgatctc gggaatgtat tccccggttc ctgggataag gttttttgcc ccactcgtgg
     3361 cgggaataag atggcttttg tggacaaaga cggcgttgaa gtagagagct caactcactg
     3421 gatgccgcta ccggaaccac cgcaggaggt gaatcaatga cctggcctga ggcattcac
//
LOCUS       DAJTVE010000252         3448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000252 DAJTVE010000000
VERSION     DAJTVE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO439_004181"
     CDS             complement(<1..143)
                     /locus_tag="OO439_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318385.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="OO439_004182"
     CDS             complement(189..530)
                     /locus_tag="OO439_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318386.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="OO439_004183"
     CDS             complement(699..890)
                     /locus_tag="OO439_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318387.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO439_004184"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO439_004184"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6318388.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3448)
                     /gene="xylA"
                     /locus_tag="OO439_004185"
     CDS             complement(2226..>3448)
                     /gene="xylA"
                     /locus_tag="OO439_004185"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6318389.1"
                     /translation="GKRMEEHLRFAACYWHTFCWNGADMFGVGAFNRPWQQPGEALAL
                     AKRKADVAFEFFHKLHVPFYCFHDVDVSPEGASLKEYINNFAQMVDVLAAKQEESGVK
                     LLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMEATHKLGGENYVLWGGREGY
                     ETLLNTDLRQEREQLGRFMQMVVEHKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYG
                     FLKQFGLEKEIKLNIEANHATLAGHSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQ
                     FPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALK
                     IAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQ
                     EQLENLVNHYLFDK"
BASE COUNT          899 a          964 c          755 g          830 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttaccca
//
LOCUS       DAJTVE010000253         3422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000253 DAJTVE010000000
VERSION     DAJTVE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..152
                     /locus_tag="OO439_004186"
     CDS             <1..152
                     /locus_tag="OO439_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6318390.1"
                     /translation="HLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            379..1644
                     /locus_tag="OO439_004187"
     CDS             379..1644
                     /locus_tag="OO439_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6318391.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1656..2522
                     /gene="ydiB"
                     /locus_tag="OO439_004188"
     CDS             1656..2522
                     /gene="ydiB"
                     /locus_tag="OO439_004188"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6318392.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2553..3311
                     /gene="aroD"
                     /locus_tag="OO439_004189"
     CDS             2553..3311
                     /gene="aroD"
                     /locus_tag="OO439_004189"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6318393.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT          881 a          772 c          824 g          945 t
ORIGIN      
        1 ctcatctgtc ccaaagaagt attgccgata ttatgtggtt cgataccgcc atcgctgcca
       61 tcggtttttt actggcactg tttatcggct tacgcagccg caaaaaaacg cggcatcact
      121 cgctaaagga aaatgtcgct ccgggtgggt aatgcaatat tcttttcagg tcatgcaaga
      181 tcttacggat aaataactct ttctgcgcta actaaggaaa atcgcgatca aaaacaaact
      241 atgacatgca atattcctgg aaacataaac tttatgccat gtacccaggg aaaatcatct
      301 tcagtatagt aattatgtaa accgtcggag aacaatacgt acggtaacga aattatcttt
      361 cagcaaggag ctgtgaaaat gtctcaaaat aaggctttca gcacgccatt tatcctggct
      421 gttctttgta tttacttcag ctatttcctg cacggcatta gtgttattac gcttgcccaa
      481 aatatgacat ctctggcaga aaagttttcc actgacaacg cgggcattgc ctatttaatt
      541 tccggtatcg gtttggggcg attgatcagt attttattct tcggtgtgat ctccgataag
      601 tttggtcgtc gggcggtgat attaatggca gtaataatgt atctgctatt cttctttggt
      661 attcccgctt gcccgaattt aactctcgcc tacggtctgg cagtgtgcgt aggtatcgct
      721 aactcagcgc tggatacggg tggctacccc gcgctcatgg aatgctttcc gaaagcctct
      781 ggttcggcgg tcatactggt taaagcgatg gtgtcatttg ggcaaatgtt ctacccaatg
      841 ctggtgagct atatgctgct caataatatc tggtacggct atgggctgat tattccgggt
      901 attctgtttg tactgatcac gctgatgctg ttgaaaagca aattccccag ccagttggtg
      961 gacgccagcg tagctaatga attaccgcaa atgaacagca aaccgttagt ctggctggaa
     1021 ggtgtttcat cggtactgtt cggtgtagcc gcattctcga ccttttatgt gattgtggtg
     1081 tggatgccca aatatgcgat ggcttttgct ggtatgtcag aagctgaggc attaaaaacc
     1141 atctcttatt acagtatggg ctcgttggtc tgtgtcttta tttttgccgc actactgaaa
     1201 aaaatggtcc ggcccatctg ggctaatgta tttaactctg cgctggcaac aataacagct
     1261 gccattatct acctgtaccc ttctccactg gtgtgtaatg ccggcgcctt tgttatcggt
     1321 ttctcagcag ctggcggcat tttacagctc ggtgtttcgg tcatgtcaga gtttttcccc
     1381 aaaagcaaag ccaaagtcac cagtatttat atgatgatgg gtggactggc taactttgtt
     1441 attccgctga ttaccggtta tctgtcgaac atcggcctgc aatatatcat tgttctcgat
     1501 tttactttcg cgctgctggc cctgattacc gcaattattg tttttagccg ctattaccgc
     1561 gtttttatta ttcctgagaa tgatgtgcgg tttggcgaac gcaaatttag cacccggtta
     1621 aacacaatta agcatagagg ttaaaggagt taattatgga tgttaccgca aaatacgaat
     1681 tgattgggtt gatggcttat cctatccgcc acagtttatc gcctgaaatg cagaataaag
     1741 ccttagaaaa agcgggattg ccatttacct atatggcctt cgaagtggat aacgatagct
     1801 ttcccggagc aattgaagga ttaaaagccc tcaaaatgcg cggaactggt gtgtcgatgc
     1861 cgaacaaaca actggcgtgt gaatatgttg atgaattaac gccagcagcc aaactggtgg
     1921 gtgccatcaa caccatcgtt aatgatgatg gctatctgcg tggctataac accgacggca
     1981 cgggccatat tcgcgccatt aaagagagcg gttttgatat caaaggcaaa acgatggtgc
     2041 tgttaggggc cggaggtgcc tcaacagcga ttggcgcgca gggggcaatt gaaggtttaa
     2101 aagaaattaa actctttaac cgtcgggatg agttcttcga taaagccctc gccttcgcgc
     2161 agcgggttaa cgaaaacacc gattgtgtcg tcacggttac cgatctcgcc gatcagcaag
     2221 cctttgctga agtcctggct tccgccgaca ttttaaccaa tggcacaaaa gtaggtatga
     2281 acccccttga gaatgaatca ttggttaatg atatcagtct gttacatccg ggactgctgg
     2341 tcactgaatg cgtgtataac ccgcatatga cgaagttatt gcagcaggca caacaagcag
     2401 gttgcaaaac gattgatgga tacggcatgt tgttgtggca aggggctgaa cagttcacgt
     2461 tatggactgg caaagatttc cctctggaat atgttaaaca ggtcatgggg ttcggtgcct
     2521 gacaggctga ccgcgtgcag aaagggtaaa aaatgaaaac cgtaactgta aaagatctcg
     2581 tcattggtgc gggcgcacct aaaatcatcg tctcgctgat ggcgaaagat atcgcccgcg
     2641 tgaaatccga agctctcgcc tatcgtgaag cggactttga tattctggaa tggcgtgtgg
     2701 accactttgc cgacctctcc aatgtggagt ctgtcatggc ggcggcaaaa attctccgtg
     2761 aaaccatgcc agaaaaaccg ctgctgttta ccttccgcag tgccaaagaa ggcggcgagc
     2821 aggcgatttc caccgaggct tatattgctc tcaatcgtgc agccatcgac agcggcctgg
     2881 ttgatatgat cgatctggag ttatttaccg gcgatgatca ggttaaagaa accgtcgcct
     2941 acgcccacgc gcatgatgtg aaagttgtca tgtccaacca tgacttccat aaaacgccgg
     3001 aagccgaaga aatcattgcc cgtctgcgca aaatgcagtc cttcgacgcc gatattccta
     3061 agattgcgct gatgccgcaa agtaccagcg atgtgctgac gttgcttacc gcgaccctgg
     3121 agatgcagga gcagtatgcc gatcgtccaa tcatcacgat gtcgatggca aaaactggcg
     3181 taatttctcg tctggctggt gaagtatttg gctcggcggc aacttttggt gcggtaaaaa
     3241 aagcctctgc accagggcaa atctcggtaa atgatttacg cacggtatta actattttac
     3301 accaggcata agcaataata tttcggcggg aacaccctcc ccgccgaact taaaaaatat
     3361 atttaaccgt atttaataaa aatatttcgt gagtctctgt gcgctaattc tccatctggc
     3421 gt
//
LOCUS       DAJTVE010000254         3368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000254 DAJTVE010000000
VERSION     DAJTVE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..628
                     /locus_tag="OO439_004190"
     CDS             <1..628
                     /locus_tag="OO439_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6318394.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKL
                     MRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(683..781)
                     /locus_tag="OO439_004191"
                     /pseudo
     CDS             complement(683..781)
                     /locus_tag="OO439_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(827..1009)
                     /locus_tag="OO439_004192"
     CDS             complement(827..1009)
                     /locus_tag="OO439_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318395.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            1108..1998
                     /locus_tag="OO439_004193"
                     /pseudo
     CDS             1108..1998
                     /locus_tag="OO439_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2001..2534
                     /locus_tag="OO439_004194"
     CDS             2001..2534
                     /locus_tag="OO439_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6318396.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2563..3090)
                     /locus_tag="OO439_004195"
     CDS             complement(2563..3090)
                     /locus_tag="OO439_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6318397.1"
                     /translation="MMHLKNIKAVNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3092..>3368)
                     /locus_tag="OO439_004196"
     CDS             complement(3092..>3368)
                     /locus_tag="OO439_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6318398.1"
                     /translation="SGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYKNVW
                     RDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          950 a          744 c          732 g          942 t
ORIGIN      
        1 aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg
       61 gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg
      121 taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag
      181 tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc
      241 tggggaatca aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc
      301 gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa
      361 tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac
      421 tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact
      481 gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt
      541 aacacatata ctctcagagc atttcgagaa tgttaaaacc agggttcaag gtactgtagt
      601 aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg
      661 tgctgcggca taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag
      721 gtgcagttga tgtatcaaca cggttcagca acacccgata ctttttctag gcttccacca
      781 ccagcacgac aagatgccgc atacagtgcc ccagtcagtc cagttttcag acaaccagtg
      841 cgtcaccttt ttgaaggcgc tttaaagcac gttttaatcc aggtcggcct gtccttgttc
      901 cgcttaattt atcttcaaat atttgttcat atcctgcaca aacaagagcg tttcgttgca
      961 ggtctgtatt ctggtcattt gttgataccc ttacatagcc aatcagcacg ctgaatctcc
     1021 cgtccaaaag cacaaatcat gccatgcagg ccagaaaccg ccattatcta aaacctcggt
     1081 ttacgagaac tcggcacaag cggggagaaa ataccgttac tcagtacagc gaatacctgg
     1141 actaatcgac aaacattcag cggtggcctt tctggtgaac tatccggcaa tgcttctaca
     1201 gctgcaaaat taaaaactgc caggaaaata agcaatgtgg cttttgatgg ttcctccgat
     1261 atcacattaa aagcaagtca tgttggtgcg tttgccttag ggaaaacagg aagcaccgtt
     1321 gcgaatgata aagcagttgg atggaactgg agtagcggag cctataacgc aactattagt
     1381 ggtgcatcaa cgttaattat tcatttttat atgggagaag gaagttgtcc tgcagctcag
     1441 tttcggatta attataaaaa tggcggtatt ttttatcgtt cagcccgtga tggttatggt
     1501 tttgaagccg actggtccga attttacacc accagaaaac cttcagcagg agatgttggt
     1561 gcactgccgt tatctggtgg tcaactgaat ggtgcactgg gtatcggaac atccagtgct
     1621 cttggcggta attcgattgt attgggtgat aatgacacgg gctttaaaca aaaaggcgat
     1681 ggtaatctgg atgtttatgc taataacgtc catgttatgc gctttgtctc cggaagcatt
     1741 caaagtaata aaaccataaa tattacggga cgtgttaatc cctcggatta cggtaacttt
     1801 gattcccgct atgtgagaga tgtcagactt ggcacacgag ttgtttagac catgaagaaa
     1861 ggcgtgatgt atgaaaaatc aggccatgta atcacggggc ttggtattat cggtgaagtc
     1921 gatggtgatg atccggcagt attcagacca atacaaaagt taattaacgg aacatggtat
     1981 aacgtatcgc aggtataatc atgcagcatt taaaaaacat tgtcgcaggt aatccaaaaa
     2041 ccgttgaaca ataccagcta acaaagaatt ttgatgttat ctggttatgg tccgaagatg
     2101 gaaaaaattg gcatgaggaa ttaaaaaact ttcaggagga tacaataaaa ctggcttaca
     2161 cagttgaggg aataattatt gctatagaca aggatgtatc agcaattaat ccagaaggtt
     2221 taagtgtcgt tgagttgcct gatattacag caaatcgccg ggctgatatt tcgggcaact
     2281 ggatgttcaa agatggtgcg gtgataaagc gaacttatac cgaggaagag caaaggcaac
     2341 aagcggaaaa tgaaaagcag agcctgctgc agctcgtcag ggataaaacc cagctgtggg
     2401 actctcagct acagctgggc atcatttccg atgagaataa acaaaaatta accgagtgga
     2461 tgctctatgc gcagaaggtt gaatccacag acacctccag cctgccagta acgtttcccg
     2521 aacaaccaga atgagagaag gcccgctatc gggccttaat ttttattcag gcttttgtgg
     2581 ccattcagga tttgccgtat ccacacggct gaccagaaca ctgtagcgtt cccatgcttc
     2641 cagtcgtgtg cgttcctcgt ctgttgccat attcagcctg acagctcgtt ccagcggctg
     2701 gatgactgat tcagcttcgg aaagcaatgc ggccttttgt gattcggcct gttgttgcag
     2761 ttcctgttct gtatattccc tctttactac tttaccatcc agaaacatcc attttccgtt
     2821 attgtctgcg tgacgatttg ccgtaatatc aggaacttca actatgctta gcccttcagg
     2881 attaatagtt gaaacgtctt tactgacagc aacgatgatg ttgttctcat cgtaagcaat
     2941 ttttatggtg tctggctgaa aattcttttg ttcctcatac cagtttttgc catcttcgca
     3001 aaagagccat accacgcccg cttttttagt tagctggtat tgttccggtg ttttcggatt
     3061 cacggcctta atatttttca ggtgcatcat cttaaacact ccccacgttg taccatattc
     3121 ccccaatcaa tttctgaatt ggccttctgg caacagtgtc gacaatatca tccgcattgc
     3181 tgttaattgc ggctgttaaa acataacctg acgcatcgcc aaagccgtag tcgcgccata
     3241 cgtttttata ttcaatgctg ccaagacgaa tatcccgaac gacatgatta tgcagccatg
     3301 tgcttaacca gttgttttcc cacactgaac cataaatatc gccactagac gcaacttgcg
     3361 caccactt
//
LOCUS       DAJTVE010000255         3351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000255 DAJTVE010000000
VERSION     DAJTVE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(28..228)
                     /locus_tag="OO439_004197"
     CDS             complement(28..228)
                     /locus_tag="OO439_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318399.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(228..500)
                     /locus_tag="OO439_004198"
     CDS             complement(228..500)
                     /locus_tag="OO439_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6318400.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(503..781)
                     /locus_tag="OO439_004199"
     CDS             complement(503..781)
                     /locus_tag="OO439_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318401.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(782..1339)
                     /locus_tag="OO439_004200"
     CDS             complement(782..1339)
                     /locus_tag="OO439_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318402.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(2903..>3351)
                     /locus_tag="OO439_004201"
     CDS             complement(2903..>3351)
                     /locus_tag="OO439_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318403.1"
                     /translation="DEAVSGSVKASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDV
                     LSTVKALQAKGVTVISLREGFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAK
                     AEGVHMGRPVKASSEAVQTLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          928 a          697 c          619 g         1107 t
ORIGIN      
        1 ttttctaatt tctcttaaag tctcggacta ccaataacct aattccgctt tatttcgttc
       61 aaaccattcc gctgtaactg ctgtctttag tgttgcgttt gggctggtgg catgaatgaa
      121 gatatctttt ggattgcaag catcaccgaa accaccttct ttgatgagca ggtctgatac
      181 atgcttaatc attttatctg tcaaatattc gaatttaata cataacatta aatctccagg
      241 agtaaattgt ttgggtggca atttctttta ttaccatccc tgaagataat ctttttagtt
      301 gcctgaactt catgatgcaa aaaccagatg acatacttca gataataatc acgagtgcca
      361 taacgaatca ttggattacc gtaagcatca tctcttgtct taacctctcc accatcaatc
      421 cattcaagca ttcctgtctc ataattaatc atgaagtaag ttgcaaagaa ccgtttaata
      481 tcatctcgcc attgcttcat aatcagacct cctgagacag tttgtgaatt ttgatactta
      541 gtaaccttgc taaatccagt ccaaaagcat ttaaggattt cagactatca aggtcatctt
      601 gcttaatcct gatatggtgt cttgcagcct ctaagcgact attaagcaaa tcctgttcgt
      661 taatatcacg gttaattgca agcttcatca ctgctgagtg tggcagacct gtaatgtttt
      721 tgattcggtt cagttcatca aaggctgtct ttgtaactct ggttaatgtg tgtttattca
      781 tttaaaaatc ctctgtaact aaataggggt caaactcgaa atctggttgc tcgttttcca
      841 ttggctcaat attcccaacg gcaatatgat gttcaacaat gccaattaat ttcgcgcaca
      901 tcacagcaag caaagcgcaa tatttatcgc ttggtaattc gtaactaagt ctggttgcct
      961 gtcgctgaag atgatttgtc acggtggatg cgttaatttc gtcaagattc tttagcagat
     1021 aaaatagttt tttgttgtat ttcgggtggc cttcacttct acagaaccca ataaaaacat
     1081 catcaagctt gcctggtaac tgctcgtaac tgtcaaagtt agccagataa tttttaatag
     1141 tattctttgt tgttttgcgt actggcataa atctgtctcg tggcgtaatg ctacgaatga
     1201 ccgcttcaat gttcttacgt tcggatttgc taaaggttgg gacttcgtag ctggtggggt
     1261 tgtttgtgta agcgtaggag ttgtgcttgt tgcgttctct tgatgcctga agcatcgctc
     1321 taaattctga atactgcatt catgttctga ctctcataag atgttattat tctgttaacc
     1381 tatatacaat attagcattt tcaaagacta atgtcaagta tttttaacat tttcgtaaca
     1441 tctgtggtat aatgggttta tggggagagt aaaatatgag gcgtggctat gtatttggtc
     1501 gcatattttg cattgcacac agacctcttc aatcgccctg taacgagtta aacgacctct
     1561 tcaggtcaac tcataaggtc aggtactttt tgtgtcagaa tgcgattgag agcgtcttac
     1621 aaggaaaact aatggtaaat ctccctcctg agctggggtc acctgatgta tggactggtg
     1681 gaggtattgg gctgattgca ggcctgctac ttcgctacgt cctcgtcaga gtgcttgttg
     1741 tctttaaact gttcggtcgt acatggacgc ttgaccttaa ggataacgac cacacagacc
     1801 cccgttaaac tccttcaaaa cccctgcacg agatgacaga tagaagtcta tggtacaggg
     1861 gtgtctttag cattttgtgc tatctattaa aaaaatagct tgacagaacc ggaaagccgt
     1921 atctatcaag cattcacctc tctgacagta atttctaaaa gttcttattt ttcaataagt
     1981 tgtaattagc catatataga gaactaaaag attttttagc tattttgttt aaaagcatat
     2041 caaacgatac attttattga tttaggtcaa agacaaaagc attgacaaaa tggtaagatt
     2101 gttcacatga ccttacttaa cagactttta agagactttg aaacagcctg tttaacaggt
     2161 cactacctct ggtcttatcg ttcccttctt tctaggtggt tatctgttaa gagtttttca
     2221 gtttttattc ttaagataat tgttaagatg atactctccc ttcgggttat atcgtatctc
     2281 ttctcccgtt aaatatccct tcacagtcag actaaataac acctgtgtag ttcttggtag
     2341 aatctttcaa ggtaacttag ctctgagttt atgaagttga cctatgagat agactctacc
     2401 gaatctgtta gtcaattgtt gcttaggtgc tgctaaggct tacttagtgg aaagagaggg
     2461 gatatgtgac cataggggaa cccctcctgt taaaagacta cttagaagtc tgaggtttta
     2521 gtaaaatctg tgaatatggc ttctcagacg ctctgtaacg cattctaagc gattttaccc
     2581 ctttatttta aggtggttag tacatcccta taggttaccc ctaaaaatgg ggttaaaact
     2641 cgctacagag ctttctatga gattagcttc taatcgaatc aactatgagg tttggtagga
     2701 ttgtgcaaaa tctgtacagt aacttttcag aatctgaaaa tttttataaa aaatgactag
     2761 ccctctttga atctcatctt ccccgaatct tcaaaaatat ctgacagatg tctgtagctg
     2821 ctaagtaccc gtcacaccag ctatgtgacc cccttggcaa aaaagccaaa tatcagctac
     2881 atccatgtaa ctattcagta acttatttag caagtctgta ataagttgct ctagagattc
     2941 ctaactcttc ctgaatctgc aacctcgtct taccttgtga aataagcgtc tgtacagcct
     3001 ctgaagaagc ttttacaggc ctccccatat gtaccccttc agctttagca cgttcaatac
     3061 cagcctttct acgctctgct atcaagcttc tttcaagctg tgccaacgtt gacataatgc
     3121 ctaacattgc ttcacccata gcgcttgata agtcaaaccc ttctctaagg ctgataactg
     3181 tcacaccttt agcctgtagc gctttaaccg ttgataacac atcaattgtg tcacgtccaa
     3241 gacggtctac agctaccaca actacggtgt caccttctcg tgcataagct aatagtctgc
     3301 tgaaaccgtt tcttaaagaa gctttgacag aaccactgac agcttcatca g
//
LOCUS       DAJTVE010000256         3240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000256 DAJTVE010000000
VERSION     DAJTVE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..661)
                     /gene="proC"
                     /locus_tag="OO439_004202"
     CDS             complement(<1..661)
                     /gene="proC"
                     /locus_tag="OO439_004202"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6318404.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GE"
     gene            781..1239
                     /locus_tag="OO439_004203"
     CDS             781..1239
                     /locus_tag="OO439_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6318405.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1422..1946
                     /gene="aroL"
                     /locus_tag="OO439_004204"
     CDS             1422..1946
                     /gene="aroL"
                     /locus_tag="OO439_004204"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6318406.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            1996..2187
                     /gene="yaiA"
                     /locus_tag="OO439_004205"
     CDS             1996..2187
                     /gene="yaiA"
                     /locus_tag="OO439_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6318407.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2445..3122
                     /locus_tag="OO439_004206"
     CDS             2445..3122
                     /locus_tag="OO439_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6318408.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
BASE COUNT          835 a          755 c          848 g          802 t
ORIGIN      
        1 gttctcccgt ttccagcacc atttttgcgg aacccattac cgcctgagcg gcaaatttat
       61 acgcctgggc gcgtggcatc ccgcccagca cggcggcgtc ggccatcgct tcgataaaca
      121 taaatacgta ggctggcgaa gaaccgctca caccgaccac cgggtggatc atcggctcag
      181 caattacttc cgcttcgcca aagcagcgga aaatattcag cacatcagcg gtatcttcgg
      241 gggttaccag cgcgtttggc gttacggagg tcatcccggc attaaccagc gcgggagtgt
      301 tcggcatggc gcggataatt ttccggtcat ggcccagcgc gcgggcaagc tggtcgagcg
      361 tgacacctgc agcaatagaa acgaccagag agtctttatt caggctggag gtgatttcgc
      421 taagcacttt aatcatgatg ccaggtttaa cggcggcaaa aatgatgtcg gcgatttgcg
      481 ccacttcttg cgccgattct gcggcgttga tgccgaactg gtcatgcagg gcggcgactt
      541 tatccgggga gggggtgtat acccagattt gccctggaag cacctgaccg ctggcaatca
      601 gaccgccgag aatggctttt cccatattgc cgcagccaat aaaaccgatt ttcttttcca
      661 ttgcctcact cctgccgtga aattcattgt tttgataatc gctggcagaa gcataaacag
      721 aactatgccg gaaggcaaaa gcgcgacaca atagaggatt acccaacaaa ggatgacttt
      781 atgacaattt gggtggatgc cgacgcgtgt cccaatgtaa ttaaagagat tttgtatcgc
      841 gcggcggaac gtatgcagat gccgctggta ctggtagcaa accagagttt acgcgtgccg
      901 ccatcgcgat ttattcgtac gctgcgcgtc gcggcaggtt tcgacgttgc cgataacgaa
      961 attgtccggc agtgtgaagc gggcgatttg gtgatcaccg cagatatacc tttggctgct
     1021 gaagccatcg agaaaggcgc agcggcgctt aatccgcgcg gcgaacgtta cacgccagcg
     1081 accattcgtg agcgcctgac gatgcgcgat tttatggata ccttacgtgc cagtgggatc
     1141 cagaccggcg gaccagatag cctttcacaa cgtgaccgcc aggcctttgc cgcggagctg
     1201 gagaagtggt ggctggaagt gcaacgtagt cgtggctaaa tgtaatttat tatttacact
     1261 tcattcttga atatttattg gtatagtaag ggatgtattg agattttcac tttaagtgga
     1321 attttttctt tacaatcgaa attgtactag tttgatggta tgatcgctat tctcatgaca
     1381 ccggctttcg ccgcattgcg acctattggg gaaaacccac gatgacacaa cctctttttc
     1441 tgatcgggcc tcggggctgt ggtaaaacaa cggtcggaat ggcccttgcc gattcgctta
     1501 accgtcggtt tgtcgatacc gatcagtggt tgcaatcaca gctcaatatg acggtcgcgg
     1561 agatcgtcga aagggaagag tgggcgggat ttcgcgccag agaaacggcg gcgctggaag
     1621 cggtaactgc gccatccacc gttatcgcta caggcggcgg cattattctg acggaattta
     1681 atcgtcactt catgcaaaat aacgggatcg tggtttattt gtgtgcgcca gtatcagtcc
     1741 tggttaaccg actgcaagct gcaccggaag aagatttacg gccaacctta acgggaaaac
     1801 cgctgagcga agaagttcag gaagtgctgg aagaacgcga tgcgctatat cgcgaagttg
     1861 cgcatattat catcgacgca acaaacgaac ccagccaggt gatttctgaa attcgtagcg
     1921 ccctggcaca gacgatcaat tgttgatttt agagcgccta tacttaacgt tcatcccgtg
     1981 aaataaggaa gaacgatgcc aacgaaaccg ccttatcctc gtgaagcata tatagtgacg
     2041 attgaaaaag gaaagccagg acagacggta acctggtacc aactcagagc cgatcatcct
     2101 aaaccagact cgttgatcag tgaacatccg acggctcagg aagcgatgga tgcgaaaaaa
     2161 cgctatgagg accctgacaa agagtgaccg catcagactg ctcggaaggg attctgagtg
     2221 ccactacaag ggatctgcgt cacatttttc ataattcatg tttttctaat aattagaata
     2281 ttaaacaata acaatccatt actggaatca tttggaatct ttacattatg ccgtgcacgt
     2341 ctgctgctac gctttttgtc atttgtagca caagtaagtg tcagcagtgg tgctccacac
     2401 ttgcccggta attaacgacg aaagaaaagt aaggtggatg aacaatgagt gcgtcgttgg
     2461 cgatcctgac catcggcatt gtacctatgc aggaggtttt gccgctcctg acggaataca
     2521 ttgacgaaga taatatttcc catcatagcc tgctggggaa gttaagtcgt gaagaagtga
     2581 tggcggagta cgcgccagaa gcaggcgaag acaccattct cacattatta aatgacaacc
     2641 agctggccca tgtttcgcgt cgtaaagtgg agcgagacct gcaaggtgtg gttgaagtgc
     2701 tcgataatca gggttatgac gtcattttat taatgagtac agcaaacatt agtagtatga
     2761 ctgcgcgtaa tacgatcttt cttgagccgt cgcgaatatt gcctccactg gtttcctcta
     2821 ttgttgaaga tcatcaggtg ggggttatcg ttccggttga ggagatgctg accgttcagg
     2881 cgcaaaaatg gcaaattttg cagaaatcgc cggtattttc attgggtaac cccattcatg
     2941 attcagaaca aaaaatcatt gatgccggga aagaattact ggcaaaaggg gctgatgtca
     3001 tcatgctgga ttgtttagga tttcaccaac gtcatcgcga tttactgcaa aaacagctcg
     3061 atgttcctgt cttgctgtct aacgtattga ttgcacggct ggctgcggaa ttactggtgt
     3121 aattttgcgt gacagccagc gcctctggcc cctatagtga agtagatgtt caactaccag
     3181 acagggccag tttatgcttc aaagtaatga gtacttttcc ggcaaagtga aatcaatcgg
//
LOCUS       DAJTVE010000257         3238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000257 DAJTVE010000000
VERSION     DAJTVE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..234)
                     /gene="fliZ"
                     /locus_tag="OO439_004207"
     CDS             complement(<1..234)
                     /gene="fliZ"
                     /locus_tag="OO439_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6318409.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDL"
     gene            complement(280..999)
                     /gene="fliA"
                     /locus_tag="OO439_004208"
     CDS             complement(280..999)
                     /gene="fliA"
                     /locus_tag="OO439_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6318410.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1164..2738)
                     /locus_tag="OO439_004209"
     CDS             complement(1164..2738)
                     /locus_tag="OO439_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6318411.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            2993..3238
                     /locus_tag="OO439_004210"
                     /pseudo
     CDS             2993..3238
                     /locus_tag="OO439_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          825 a          773 c          696 g          944 t
ORIGIN      
        1 taaatcacca caaaatcggc acaatgccgc ccatgatttt tgttccactt gccagccatt
       61 ttcatcaagc agcatgtcca ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg
      121 aacgagggta atgcggtcga gtaatttacg gcaatgcgcg caatgagtct ggctgtgttt
      181 aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca tcattaagaa
      241 ctcctggtag tcaaaattaa agtgcggcat ttactgacgt tataacttac ccagtttagt
      301 gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag
      361 caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt
      421 ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tggcgcagat tactgtccag
      481 cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc
      541 gtgctcttcg cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcaacat
      601 ttggcgataa tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt
      661 gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg
      721 cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg
      781 ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt
      841 aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg
      901 aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg
      961 tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata
     1021 tgagttatcg gcatgattat ccgtttctgc aggtttttta atcggacgat tagtgggtga
     1081 aatgaggggt tatttagggg ttaccggtaa attgcggaca gaaaaaaccc cgccggtggc
     1141 ggggtttgag cgataagtgt aaattaacgc agcagagaca gtacgttctg cgtggtctgg
     1201 ttagcctgtg ccagaacaga ggtacccgct tgttgcagga tctgcgcacg agacatgtta
     1261 gacacttcgg ttgcgtagtc agcatcttct atacggctac gggcagaaga caggtcgttt
     1321 acggtgttgc caaggttggt gatggcagag tcgaaacggt tttgtactgc acccaaagaa
     1381 ctgcgcaact tatcaacttt tgccaaagct tcgtctaatt tggataatgg atttgtggtt
     1441 gctaaggctt tcaaatctga ggcactcagt tcagcacctt tggttgccgt cttattcgtg
     1501 atattggctg tataaacctt atcatcttca cgcataattg cctgtgcaga accatcattt
     1561 tttactgccc attcaactgc cttaccatta ggatcagtaa taacagacgt gttacctgta
     1621 gcggcatcta catttactgc aatgtaggtg ccgtctgatg ttttagcagc aaatgaatta
     1681 ggtacaggat tcccttgggt atcttgtact tggtggacat ctaggttttt tcccccaaaa
     1741 taaacagaca ttggtgcatc attataagct aatgctttac ccattttacc cgcagctaaa
     1801 tcaactgatg tggcagaacc attatcttca acaaatgcag ttacacgacc accaaagttt
     1861 gctaactgaa catccttacc acccacctga actttttcat aacctgtaga gttttgtttc
     1921 aataatgtaa aaccgttcgt gttggtatta ttatcttgac caacaaaata agtctgccct
     1981 tccacctgta cttgataaac ttttgttcct gcagtaccgg tcgcatcagt tgtatccttt
     2041 gtatcttgta cattaccggt accaaaataa gtttcaacat cagcattttt ataattgcta
     2101 ccgaatgctt tagcacctcc cacactacca tcagttgcat ttaccttagt taagtcagga
     2161 gcggcttcgc tcttaagtgc aaccgcagag gacattggaa cttttcctag tgctacgcta
     2221 aagttatcaa gccccaaggt tttagcatca attttatcaa gggcaatctc aatggtttca
     2281 ccatcgttcg cacctacttg aattttcata gtctgatcag aagccagaac ttttacacca
     2341 ttaaattgtg tctggttaga aacacgatca atttcactaa ggcgttgtgt aatttcatcc
     2401 tgaattgaat tcaggtcgga gtcagagttt gtaccattag tggcctgtac tgacaattca
     2461 cgaatacgct gtaagttatt gttgatttca gaaagcgcac cttcagtggt ctgcgcaaca
     2521 gaaataccat cattcgcgtt acgggaagcc tgagtcagac ctttaatatt tgctgtaaaa
     2581 cggttagcaa tcgcctgacc tgctgcgtca tctttagcac tgttaatacg caggccagaa
     2641 gagagacgtt caatggcgga gctcagagaa gactgagatt tgttcaggtt attctgggtc
     2701 aacagcgaca ggctgtttgt attaatgact tgtgccatga tcttttcctt atcaattaca
     2761 acttgatgtt attgggctgt tgcccacggt ttctcaccgt accccttgta tcggcacctg
     2821 aatttcgaac tttagaaaat tttagtttct ctcctgtttt ttcttaacgg ctgaaatatc
     2881 cgtaatatac aactctactc tgcgcatcat cttcgtgaat caactattaa actttgcgca
     2941 caaattgccg ataacccgct tatctactgt tttcaatcaa aaggaattag gtatggcttc
     3001 atttacatct cttggcgttg gttcgaatct gccgctggat acattgctaa acaacctgac
     3061 catcgctgaa aaaaaacgct taaacccaat tactcagcaa cagagtgaca ataccgcacg
     3121 tctgacagcc tatggcacat taaaaatgtg cattagaaaa attccagact gcaaatactg
     3181 cgttaaataa agccgatctg ttcaaaagca ctaccgttac cagtagcaat gaagacct
//
LOCUS       DAJTVE010000258         3206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000258 DAJTVE010000000
VERSION     DAJTVE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1220)
                     /locus_tag="OO439_004211"
     CDS             complement(<1..1220)
                     /locus_tag="OO439_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6318412.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYST"
     gene            complement(1217..1516)
                     /locus_tag="OO439_004212"
     CDS             complement(1217..1516)
                     /locus_tag="OO439_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6318413.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1523..1843)
                     /locus_tag="OO439_004213"
     CDS             complement(1523..1843)
                     /locus_tag="OO439_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318414.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(1836..2033)
                     /locus_tag="OO439_004214"
     CDS             complement(1836..2033)
                     /locus_tag="OO439_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6318415.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2087..2539
                     /locus_tag="OO439_004215"
     CDS             2087..2539
                     /locus_tag="OO439_004215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318416.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2597..2773
                     /locus_tag="OO439_004216"
     CDS             2597..2773
                     /locus_tag="OO439_004216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318417.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(2885..3094)
                     /locus_tag="OO439_004217"
     CDS             complement(2885..3094)
                     /locus_tag="OO439_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6318418.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          811 a          850 c          676 g          869 t
ORIGIN      
        1 gttgagtagg gggctttgtg tttcgggtca attgccacct tgtcaccgcc tgtaatggcc
       61 ttaatccctg ccccatcacc agcgtagcgg gtcatatccg gcatgataat cagcgaaaag
      121 ccaaccacta acgcgcgttc ccttgcatct tccagcgcct tcatgcttgc cgatactgtg
      181 ttggccttac ccgccagcat ggtgcaaatc tccgccatca cgcttttacc acttccccct
      241 ggacctgtta cctcaatgaa taactgccag tcgtaccggt tcgccagcac catgaataat
      301 acagccagta cgcgatccgc cttgcggtca ttctcagcca ccgaacggcg taaccacttc
      361 cagaaattcg gcgcatgtgt tgccagcgtt tccccctctg ctggtgggct gaaaggtaat
      421 tcactggcaa ttaacaacca gtcgtttttg ttatgctccc gaaaattacc tgttctggta
      481 tcaaataccc cgttactgaa tccaatcagg ttacgggctg tattccccat tacaggcaaa
      541 cttaacttca tggtatcgac cgccgattta atggcgttct gcgaatagct gatctccgca
      601 tcaatgaaaa tctgtgccat agctcgctgt aattctttat cctgtactgg ctcccataca
      661 acgccgttgt aatggtgaac agtgtcagag tcagcatgaa tcgccagttc accgccataa
      721 tgtgccagga gaacttcgcc gcgttgactt gctcccatct ggttaagcgc cagtgatgaa
      781 gcgttatcgt cttttacccg ctcttttttc tttacaggca gttcaactac ctttttcttt
      841 tccgcctgct ctgcccgttc acgttccaga tattcgcgcc agttctcccg tttctggctg
      901 tgcattcctt cagggtaata atcagcatcc ctgacacctg ccgctgccag tttctgcccg
      961 atggtattaa caagccccgg acgcaataac cccgcctggt agagacgcac ccgatagcgt
     1021 ccgtccggta cgatttgcag cttatccagt tcggcaagtt gttgttctcc aagccagaca
     1081 ggaggcacgc tgtcgccagc cagtcgcccg tcctgttcct gccactgctt cgcatgtgcc
     1141 cacgcatcat tacccgcaaa aatgatgact tccgtcattt tgtcacgcgg ctggtgtttt
     1201 aaatttggcg cttttttcat ttctgctctc tccacgcggc aatcatgttt ttcagttcct
     1261 gtagtttttt atcaacatcc atacatgaca catggttatt tctggaaagc gggatttccc
     1321 gcctgaatct gctaataaag atctccacgt tcagcgaact atgaaatgaa tagccatcac
     1381 gaataaaata cacacggtca aacatcagtt cttttaccgt tactctgtta ccgttcttat
     1441 ccagataaat agcgccgggg ataattttgg ggtgtgcata accgctggca gtcaagccag
     1501 ataaatacgt tctcatgatt atttatctca gatttgaatc agtattcgct ttctctatgg
     1561 catttaatgc atctgtggca ttttcaatgg tgcaccgtaa cgaaatatca aactgcccaa
     1621 gcattgccag taacaagcca atattaccca tgtcaatgcg catagccttt tcgtcatatt
     1681 cctcattttc tgacgcatgc cacatcaggc taccaattga cgcaacagcc attgatatat
     1741 tgtcagtagc cccatccgca gcggaataaa cctttttagc aatatcatgc tcacagttaa
     1801 aatgcggatt aatcaggtac tggtaattgg tcatgtcagg catggcacac ctcctgacga
     1861 atacgggcgg cgaatacagc aacacaacct gacgggcaac ggttacgcgc ttcgcgttcc
     1921 gtccaggcgg ttacgtggat gatttgagat tctccggcac tcagggccag aaaacgccac
     1981 acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct
     2041 cctcgtttac gttgttggtt agaagccctg cgagtggtaa cgacacttgc ggggcttcgt
     2101 cgtttcaatt gctgaattgc atgtatcagc acttgtggta ttcacattac atttaggtga
     2161 ataccatttc aagtcttttt tggtattcac ttttgtatta cactgcatcc cgttattaat
     2221 gggaggtgca aacatgtcca ggagttctgt taacaataag tcacagcaac tgaatgccag
     2281 atttccacat gaagtagtga gtggcattga ggcatcccta cagccagggg aaactaaagc
     2341 gaattttata gttacggctg tacgcggtga gatcgccagg cgccaggcag aaggaagtgg
     2401 agaaaatccc cttgtgtctt cactggatgc cctagctaat gtcgaacaaa tcggcatcaa
     2461 ggcagcggag gaaatcgggc agcttatcac cgttgcacgc gaagaactcc agcgccgcaa
     2521 ggccaaagaa tcagaataat cactatcagc gccgtggtgt gaggaactcc ggcgcattgc
     2581 tttacaggcg cacacaatga ccaacgaaga atcaaccaaa aaaacatcac ctgcacgaaa
     2641 aagacgacgc agaaagaaag agcatgaatc agaacgattc gcgccgtgtt cttttgccct
     2701 tgagaagtac cttaaagaat actcatcaaa ggagagagct tcgcagttat ggcaacgcac
     2761 tgaaacagac tgatagcatt gcccaccagc ctgatagcgg ctatcattga catgcttatg
     2821 tttagtgttt ccaaactggc gaccgcccca ggtcgccttt gttttatgtg tcatatgctc
     2881 ccctttacac tgccttacct gaattaatgc gatcccggct tttaacccat tccataacct
     2941 cggacagcag ccaccctaca gaacgaccgc ccagattaag acgtgacgga aagcgccctt
     3001 ttttctccag ttcgtaacgt gtagtgcggc acactccagt taacttacga cattcatcct
     3061 cacggattac gcgatcttca tttatttcac gcatacaaaa acctacataa aaattacgta
     3121 tataaacttt tctcttagct gtagatatat gagatcggat attacttaga ttctttttca
     3181 cctcttaaat taaaaacaca accatg
//
LOCUS       DAJTVE010000259         3196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000259 DAJTVE010000000
VERSION     DAJTVE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO439_004218"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO439_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6318419.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO439_004219"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO439_004219"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6318420.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="OO439_004220"
     CDS             complement(1715..1984)
                     /locus_tag="OO439_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6318421.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="OO439_004221"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="OO439_004221"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6318422.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="OO439_004222"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="OO439_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6318423.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          833 a          774 c          771 g          818 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatccggca tttttt
//
LOCUS       DAJTVE010000260         3142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000260 DAJTVE010000000
VERSION     DAJTVE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..280
                     /locus_tag="OO439_004223"
     CDS             <1..280
                     /locus_tag="OO439_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6318424.1"
                     /translation="SSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLT
                     RFGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            584..829
                     /locus_tag="OO439_004224"
     CDS             584..829
                     /locus_tag="OO439_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318425.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            826..1248
                     /locus_tag="OO439_004225"
     CDS             826..1248
                     /locus_tag="OO439_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6318426.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1908..3142
                     /locus_tag="OO439_004226"
                     /pseudo
     CDS             1908..3142
                     /locus_tag="OO439_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
BASE COUNT          932 a          689 c          834 g          687 t
ORIGIN      
        1 gtcgtcggta atgtgtgatg gtctgttagt gggtaatgcc gaaattattc cgttcagtcc
       61 gcgcaggtat ctttatcacg cctatctggc atatatgagg gcacacggat tcggtaaacc
      121 tgtaacactg acgcgcttcg gtaaagatat gccgggggca atggcggaat atggcaggga
      181 gtatatgaaa cggaaaacga agcacggttt gcgttcaaat gtgaccctga cagaggattc
      241 agaagactgg atgccatcat gtgcaatagg tcacaaatga cgatggaaaa aattaaactt
      301 atggaataac tgttcaccac tgttcaccct gcaataaatg tcttttatat caatatatta
      361 tagggtgaac agttatttat gaactgttca ccaaactatt cactgttcac ctttttgatt
      421 ctttattgag ctttaagggt gaacagtggt gaacagttgg tgaatagttt ttgtgaaact
      481 gttaacccat taacatcatg aataaaaagg gaaaattgca aaaggtgaac aggtgaaggg
      541 ttaaaatgca aaaattttat tttattgctg tgaggtaaag cctgtgacaa cgaagcacgc
      601 caaaaaacca caatctcacg cccttgattt gacagaacac tggctgaggg tggcgataaa
      661 aatcatcgac cgcaacgccg gggaaggata cgcgaaagca catcccgaac tgataagcgc
      721 attcatgacc acgacggcgg caaactttgc cacgctgaca gaacgggaga ttgccgaagc
      781 ggaacaggtg acaaccatca acgttaaaac cggagagcag acagcatgac agcacagata
      841 gcggcttacg ggcggctggt ggctgacccg cagttaaaga ccaccagcaa gggtacgcaa
      901 atgacgatgg cgagtatggc ggttcccctg ccgtgcagcc aggcagatga cggaacggcg
      961 acgatatggt tatccgtcct ggcgtttggc agacaggctg acgcactggc aaagcaccag
     1021 aaaggcgagc tggtgagcgt ggcgggtaac atgcaggtaa gccagtggac aggccagaac
     1081 ggagaaacgc ggcagggctg gcaggttatc gcagacagtg taatcagtgc cagaacggcg
     1141 cgaccgggcg gtaaaaaagg ccaacagggg caggccactg atgcactgaa cagggccaaa
     1201 caacaggcgg ggaatgatga tccgtacggg gataacatac cgttttaagc aacgagtgac
     1261 agaagccgga gcaatccggc ttttttgtag gtactcctgg tgggggtggc ctgtccacgg
     1321 ggcgggcggt tcgcggaaaa aggcgtattt tttgattttt atgtcaccat caccaccagt
     1381 ttaagttatt gatatatggc aaaataaaaa tttttagtgt cgaattaggt tgttttttgt
     1441 tcgacgccgg aacgttcccg aaagcattta caaaaaacag gcgcaaaaaa aagcgccccg
     1501 atggtgccac cggaggcgct ttgtcagcga caaaggatac tgccactacc gaaatgatga
     1561 gtcttaattg tgctggcgtg gcaataatta gtcaattctt ttgctggtat gacaaaaatc
     1621 agaaaacaca taatctgatt tttagataga aaatgattta tttaccagtt ttatcgatca
     1681 ataatgatgc ccgttaatca taacggaggc agacttatgc cagagaacaa cagcagaaaa
     1741 ccggataaaa gtgcaacagt gcacatagac gccggaacta tggagaagct cgaacgctat
     1801 cagcagttca ttaaaagtca tcaccctgga atgccagtac ccacgaaagg gcagattgcg
     1861 agaagcgcgg ttgaatactg gtacagggca acgttaggag cctggctatg aaaacatggt
     1921 tttccattaa ggctatggct gatgttgtac atgtacgcat ttatgacgag atcggcgggt
     1981 acggtgtaaa agcatcggca cttactgacg agatcaacgc gtgcggtaat gtgcctgaaa
     2041 tccatcttcg catccattca cctggtggcg acatctttga agggctggct atctataacg
     2101 ccctgaaaaa tcatccggca aagaaaattg tacacattga aggcatggcg gcttctatgg
     2161 cctcgtttat tgccatgtgt ggcgatcaca tcgttatgcc tgaaaacgcg atgatgatga
     2221 tacatgcccc ccgtggtgtt actgccggag tgtcgggcga cgttcgccgc tttgctgacc
     2281 tgatggacaa gctgggcgac acgatggcgg aaacctacgc cggaagaacg ggcaggagca
     2341 aacaggaaat caccgccatg atggaggcgg aaacctggat ggatggcaat gagtgtaagg
     2401 ctaatggctt cgcagatgag gttatacccg cgattacagc aatggccaga attgaatcaa
     2461 aacgaatcgg agatttttcg aatatgccgg aaaatattaa aagcatgatc agccagaaaa
     2521 ctggcagcgg agaacaggaa cgactcaacg ggatacgtga attgtttggc acgttcaatg
     2581 gaagatataa cgacctggct ataagttgtc ttgctgattc aggctgtagc gttgaggatg
     2641 cacgcgaacg ccttttactc actatgggta aagaatcgac accgacaaac aaaaccaccc
     2701 ccgcaaatct ttactacgcg tacacggata acggcaacat aaccggcgat gcaatgcgtc
     2761 aggggcttaa tgcgcgtctg ggacatgaac aggcagaacg cggcaaccct tacgccatga
     2821 tgagcctttt cgatatggca caggcatcat taacccatcg cggcataagc acaggcagct
     2881 acagcacacg ctcgcagata gtaaacgcgg cattcaccca cagcagcagc gattttactt
     2941 atatccttgc tggtggtgct gaaaaatccg tgaacacagc ggcgaaacgt tccgccagtg
     3001 gacgaaaaaa ggttcattgt caaacttcca tgaggcacgt cgtgttggcc tgaatggctt
     3061 ctctacatta aacaaagtgc cggaaggcgc ggaatataaa tacatcacca ccagtgaccg
     3121 tggcgaacca atcgcgctgg ca
//
LOCUS       DAJTVE010000261         3110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000261 DAJTVE010000000
VERSION     DAJTVE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1255)
                     /locus_tag="OO439_004227"
     CDS             complement(<1..1255)
                     /locus_tag="OO439_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6318427.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="OO439_004228"
     CDS             complement(1412..1621)
                     /locus_tag="OO439_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318428.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="OO439_004229"
     CDS             complement(1644..2354)
                     /locus_tag="OO439_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6318429.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3110)
                     /locus_tag="OO439_004230"
     CDS             complement(2870..>3110)
                     /locus_tag="OO439_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318430.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          912 a          654 c          507 g         1037 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca
//
LOCUS       DAJTVE010000262         3093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000262 DAJTVE010000000
VERSION     DAJTVE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="OO439_004231"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="OO439_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="OO439_004232"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="OO439_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6318431.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..>3093
                     /locus_tag="OO439_004233"
     CDS             2834..>3093
                     /locus_tag="OO439_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318432.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVA"
BASE COUNT          761 a          693 c          797 g          842 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gat
//
LOCUS       DAJTVE010000263         2919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000263 DAJTVE010000000
VERSION     DAJTVE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..495
                     /locus_tag="OO439_004234"
     CDS             <1..495
                     /locus_tag="OO439_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6318433.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="OO439_004235"
     CDS             495..1085
                     /locus_tag="OO439_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6318434.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1072..2112
                     /locus_tag="OO439_004236"
     CDS             1072..2112
                     /locus_tag="OO439_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6318435.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2115..2660
                     /locus_tag="OO439_004237"
                     /pseudo
     CDS             2115..2660
                     /locus_tag="OO439_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2684..>2919
                     /locus_tag="OO439_004238"
     CDS             2684..>2919
                     /locus_tag="OO439_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6318436.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          757 a          637 c          833 g          692 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattaccc ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacgggactg
     2881 gcgcgacgag tactgtgttg gcgacattgc tgtatacga
//
LOCUS       DAJTVE010000264         2860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000264 DAJTVE010000000
VERSION     DAJTVE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..849
                     /gene="hyi"
                     /locus_tag="OO439_004239"
     CDS             <1..849
                     /gene="hyi"
                     /locus_tag="OO439_004239"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6318437.1"
                     /translation="LRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEEL
                     KQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKIN
                     CLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQA
                     LKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEI
                     NYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQRQ
                     KVVSPP"
     gene            768..1946
                     /gene="allB"
                     /locus_tag="OO439_004240"
                     /pseudo
     CDS             768..1946
                     /gene="allB"
                     /locus_tag="OO439_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2003..>2860
                     /locus_tag="OO439_004241"
     CDS             2003..>2860
                     /locus_tag="OO439_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6318438.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGF"
BASE COUNT          639 a          676 c          784 g          761 t
ORIGIN      
        1 ttacgtttct ctgctaattt atcgatgtta tttggtgaat atgattttct cgcccgtttt
       61 gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta tgtttcctta tgactacgac
      121 attgaagaat taaaacaggt gctggcgagt aataaactcg aacatacgct gcacaatcta
      181 ccggcgggtg actgggcggc gggggagcgc ggtattgcct gtattcctgg ccgtgaagaa
      241 gagtttcggg atggcgtagc agcagcgatt cgttatgccc gtgcgctggg gaataaaaaa
      301 attaactgtc tggtcggtaa aacgccggct ggtttcagca gtgaacagat tcacgcaacg
      361 cttgtagaaa acctgcgtta tgccgcgaat atgctgatga aagaagatat tttattactg
      421 attgaaccta ttaaccattt tgatattcct ggtttccatc tcaccggaac tcggcaggcg
      481 ctgaaattga ttgatgatgt tggttgctgc aatttgaaaa ttcagtatga catttatcac
      541 atgcagcgga tggaaggtga attaaccaac accatgactc agtgggccga taaaattggt
      601 cacctgcaaa ttgctgataa tccgcatcgc ggcgaaccgg ggaccggaga aattaattat
      661 gattatctct ttaaggtaat cgaaaattct gactacaacg gttgggttgg gtgtgaatat
      721 aaaccccaaa ccaccacgga agccggttta cgctggatgg atccgtactt tctgaaccgg
      781 gtcgtagcca ctgggaaggt tatgaaaccg gtactcgcgc agcggcaaaa ggtggtatca
      841 ccaccatgat cgaaatgccg ctcaaccagc tgcctgcaac ggttgaccgc gcttcaattg
      901 aactgaagtt cgatgccgct aaaggcaagc tgactatcga tgcggcacaa ctcggtggcc
      961 tggtgtctta caacatcgac cgtctgcatg agctggatga agtgggcgtt gtcggcttca
     1021 aatgcttcgt tgcgacctgt ggcgatcgcg gtatcgacaa cgacttccgt gatgtaaacg
     1081 actggcagtt cttcaaaggt gcgcagaagc tgggcgaact ggggcagccg gtgttggtgc
     1141 actgcgaaaa cgcgctgatc tgtgacgcac cgggcgaaga agcgaaacgt gaaggtcgcg
     1201 taactgccca tgactatgtg gcttcgcgtc cggtatttac cgaagtggaa gcgattcgcc
     1261 gcgtgctgta tctggcgaaa gttgccggtt gccgtctgca cgtttgccat atcagcagcc
     1321 cggaaagtgt tgaagaagtg actcgtgcac gtcaggaagg tcaggatgtt acctgtgaat
     1381 cctgcccgca ttactttgta ctggataccg atcagttcga agaaatcggt actctggcga
     1441 agtgttcacc gccgatccgc gatctggaaa accagaaagg catgtgggaa aaactgttta
     1501 acggtgaaat cgactgcctg gtttccgacc actcaccatg ccctccggaa atgaaagccg
     1561 gcaacatcat ggaagcatgg ggcggtattg ccggtctgca aaactgtatg gacgtgatgt
     1621 tcgatgaagc ggtacagaaa cgcggaatgt ctctgccaat gttcggcaaa ttaatggcga
     1681 ctaacgcagc agatattttc ggtctgcagc aaaaaggccg tatcgcccca ggaaaagatg
     1741 ccgacttcgt cttcattcag ccgaatagca gctatgttct taccaatgac gatctggaat
     1801 atcgccacaa agtcagcccg tatgttggcc gtaccattgg cgcgcgtatc acgaaaacca
     1861 tcttacgtgg tgatgtgatt tacgacattg aacagggctt ccctgttgcg ccgaaaggtc
     1921 aatttatcct taaacatcag cagtaatctg gcccctgcaa tgcccgtcct tgcggcgggc
     1981 attctccggt taaggtgtgt ttatgttcaa ttttgcagtc agccgcgaaa gcctgttatc
     2041 aggatttcag tggtttttct ttattttttg caacacggtt gtggttcctc ctacgctact
     2101 ttctgctttt cagttgccgc aaagtagcct gcttacgctc acgcaatatg cttttcttgc
     2161 taccgcactg gcctgcttcg ctcaggcgtt ctgcggtcat cgtcgcgcta ttatggaagg
     2221 gccgggtggc ctgtggtggg gaaccatcct tactatcacc cttggcgaag catcgcgcgg
     2281 gacaccgatc aacgatatcg ctaccagtct ggcggtgggg attgcactct ccggcgtgct
     2341 gacgatgttg attggtttta gcggattagg ccatcgcctg gcacggttat ttacgccgtc
     2401 ggtgatggtc ttgtttatgt tgatgctggg cgcacagctg accactatct ttttcaaagg
     2461 tatgctcggg ctgccgtttg gcatagccga cccatatttt aaaattcagt taccaccgtt
     2521 cgcgctctcg gtggcggtga tgtgcctggt actggcgatg attatctttc tgccgcaacg
     2581 ttttgcccgt tatggcctgc tggtcggcac cataaccggc tggttgttgt ggtacttttg
     2641 ctttccttct tcgcattcgc tctccggtga gttgcactgg cagtggttcc cgctcggcag
     2701 tggcggtgct ttgtcgccgg gaattattct gacggcggtg attacaggtc tggtaaatat
     2761 cagcaatacc tacggtgcga ttcggggcac ggatgttttt tatccgcagc agggcgcggg
     2821 gaatacgcgt tatcgtcgca gctttgtggc gaccggattt
//
LOCUS       DAJTVE010000265         2853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000265 DAJTVE010000000
VERSION     DAJTVE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(4..318)
                     /locus_tag="OO439_004242"
     CDS             complement(4..318)
                     /locus_tag="OO439_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6318439.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(323..538)
                     /locus_tag="OO439_004243"
     CDS             complement(323..538)
                     /locus_tag="OO439_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6318440.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(689..2542)
                     /locus_tag="OO439_004244"
     CDS             complement(689..2542)
                     /locus_tag="OO439_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6318441.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          660 a          804 c          688 g          701 t
ORIGIN      
        1 aagctaacaa cccgtaatcg agttatcaga actgttaatt tttatggtat accgcgcctc
       61 tgaacagggg cgcgtttctg gcaacagctc gtccccttca cataacccgg cagcaacatc
      121 caggaagacc tgtctgatgc tccttctggc tgctgcctca taaaactcca gcgcggcacc
      181 ttcaacacgg tccagcgaga tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc
      241 ccgtaacgct aaagttaccg taactttatt ctcaaaattg cggatccctt tcacaatcag
      301 ttcatagttt tgagtcattg aattactctc cccgtgcagc cttacgacgg tcctctctga
      361 ttttgaaata caggttagtc agatatgtca gcagcccaaa cagcagactc cccagcacgc
      421 ctattgccgc ccactgagac ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg
      481 ttcccaccgc tgacgtggtg tatgacacac ctgttgtgat tttttccatc tggtacatac
      541 cccgtctccc gttatccgga agctgacaac aataaaaaaa gccaccagtt aagtactgat
      601 ggctctgata actcatgcag gcatctcaga cgacccactg acactaccgg tgagtttaac
      661 gataccttcc atttggctgg ctcacttttt atgatgatgc cggtgcattt atctccagca
      721 ccagactttc tatctcaacg ccatacgctg catttttggt aatatccgtc agcgtcagcg
      781 cattcagccc cagtgtcaga ctgtctttta tgacctggaa tgccgggcca gccactccat
      841 tcagtttcgg agtaaccgtg gcactgccgg cggtgaacac cagctccagc gtcagccagt
      901 cgttactgta attcccgaac tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca
      961 gattcagttt gccgtctgtg gtctgggtga agaacgacat caggaacggg ttaccagtcc
     1021 cggtcatcgc cacgacgtca ggtaacgcta catcggtata cagataaatt cccagaccga
     1081 actggttgtt ggtcagtgcg cctgacagtc gaaacttaca gctcagtctg ccaccccgtg
     1141 tcagcaggga gactgcgtca tccaccgggc gcgtcaggga ccaggcttta ttgctctgct
     1201 tggtgatctt aaatacacca tctgacaact gaactccgcc attcttaatg ctccagccct
     1261 gcgcagcagc gtctccggct gtcggcaaca gggagattgt gcgtatggat gcatcttcag
     1321 acggccccga tggcgtgtcg ccgccgggcg agggtttgat ttccggtgcc ttaccactaa
     1381 tgaaggctaa ggtgcgaccg gctacgttca gaatagcagt tgccatacga tcgggaataa
     1441 tgccacgacg cgcccatgag ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt
     1501 tcgttcggga tgccgaaccg taataaccag acccggcaat atcaggatct tctgacgggt
     1561 tgtttgtcgg tgtattaact ccgctaccat cggtcataaa gggaacaaaa taaatctgct
     1621 gggattcttt acctttatat gcaccatata ccacttcata ttgcgtaccg tgttcttgtt
     1681 tccacgcgta tgtcgtgtcg ccacaaatcc aggggactga tgccggactt ccaccgtgac
     1741 actgcgccgc cagcccggca aggtcagcac ggaactgctg taccattgca agaaatgctg
     1801 ctggctgctg ggcgtaactg gcattcgtca tatcgaattc cccctgcatc cagcatatcg
     1861 ccagcaaaac gtttttcggg tttttctgca atgctgcctt cgtgcggaaa agcagatcct
     1921 gatataacgg cttacccact ccccagcgag ccgaatcctg actggccccc gtggactcgc
     1981 tgaatgtccc ctccgtgccc tgggtgaatg ccgaaccacc acgacagcat ggtaccagca
     2041 ggatccccgc attattaggg atatacggaa gcagtttttt ggcaatatgt aagccctgtc
     2101 cgacacagcc gtactgccct ttgctcaggt cagcccgggg atggttaatc gtactcatat
     2161 cctgaacatc atgcagacaa tggtcagcag gaatgatgtc gttaaatacg catacttcac
     2221 caccgggagt cactgtgtta cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt
     2281 atgaatccgg aagcggaaga ccttcaccgt aagccatggc attggattgc ccggccagta
     2341 cgatgacgta gtaccactcc ggctcagttg caccactgac gaccacatca ccttctgctg
     2401 caatcgcctg catcagggta taaggggtta tggccaccgg actaccaaac ggctgccagc
     2461 cctccttcag tttttgtgtc agtcgtttcg caaggtctga cggcgatgcc gccctgacca
     2521 cgtcatagtg tttaaatgcc atgaatcctc ccggccggga taatattgtg agtaaaataa
     2581 ggagcgggct gaagtccgga agttacagga caatggcaga agagagacga cagcccgcaa
     2641 ttcgaaaaag accgcgcagt tgcgcagagt gattactatg gggtattatt cgccagctga
     2701 aatattactt cacgttttat tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt
     2761 ttttgcccac aagaaaaccc ctccggagag gggctaaagc cgcgtatctg tatcatcatg
     2821 cacatggtgc cgggtgcctc ccggtgagtt cag
//
LOCUS       DAJTVE010000266         2818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000266 DAJTVE010000000
VERSION     DAJTVE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..902
                     /gene="bglB"
                     /locus_tag="OO439_004245"
     CDS             <1..902
                     /gene="bglB"
                     /locus_tag="OO439_004245"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6318442.1"
                     /translation="LWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            988..2604
                     /gene="bglH"
                     /locus_tag="OO439_004246"
     CDS             988..2604
                     /gene="bglH"
                     /locus_tag="OO439_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6318443.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2631..>2818
                     /locus_tag="OO439_004247"
     CDS             2631..>2818
                     /locus_tag="OO439_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318444.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          796 a          589 c          694 g          739 t
ORIGIN      
        1 cgttatggct gacgtttaat gaaatcaata tgtcgttaca cgcgccattc acgggcgtgg
       61 ggctggcaga agagagtggc gaggcggaag tttatcaggc gatccatcat caactggttg
      121 ccagtgcgcg ggcagttaaa gcctgtcata gcctgatccc cgaagcgaaa atcggcaata
      181 tgcttctcgg tgggctggtt taccccctca cctgccagcc acaggatatg ttgcaggcca
      241 tggaagagaa ccggcgctgg atgttctttg gtgatgttca ggcgcgtggc cagtatcccg
      301 gctatatgca gcgtttcttc cgcgaccaca atatcaccat tgagatgact gaaagtgacg
      361 cagaagattt aaaacatact gtagatttca tctcttttag ttattacatg actggttgtg
      421 tttcccacga cgaaagcatt aataaaaatg cgcagggcaa catactgaat atgatcccca
      481 atccgcatct gaaaagttca gagtgggggt ggcaaattga tccggttgga ttacgggttc
      541 tgttaaatac gctttgggat cgttatcaaa aaccgttatt tattgtcgag aacggattag
      601 gcgcaaaaga cagcgttgaa gcggatggtt cgatacagga cgattatcga attgcctatt
      661 taaacgatca cctggtacag gtaaatgaag cgattgccga tggtgtggat attatggagt
      721 acaccagttg ggggccaatt gatttagtca gtgcatctca ttcacaaatg tctaagcgct
      781 acggctttat ttatgtggat cgtgatgata atggcgaagg aagcctcaca agaacgcgta
      841 agaaaagctt cggatggtat gcagaagtga tcaaaacgcg ggggctgtca ttaaaaaaat
      901 aaccaataaa gcaccttaat tatcgtcgca ttcagaacag tctggatgcg atacgttaat
      961 tctttctttg caccataaag ggatattatg tttagacgaa atcttattac ctctgccatc
     1021 ttattaatgg caccgttagc cttttctgca caatcattgg ctgaatcatt aacggtggaa
     1081 caacgccttg agttattaga aaaagcgtta agagaaacgc aaagcgaact caaaaagtat
     1141 aaagatgaag agaagaaaaa atatacgcca gcgacggtga atcgtagcgt aagtacgaat
     1201 gatcaagggt atgccgccaa tccgttcccg accagtagtg ccgcaaaacc tgatgctgta
     1261 ctggtcaaaa atgaagagaa aaatgccagt gagacaggtt cgatttattc ttccatgact
     1321 ctgaaagatt tcagtaagtt tgtgaaagat gaaattggct ttagttacaa cggctattac
     1381 cgttctggtt gggggaccgc ctctcatggt tcacctaaat catgggcgat tggttctctg
     1441 ggccgctttg gtaacgaata ctccggctgg tttgatttgc agttaaaaca acgtgtctac
     1501 aacgaaaacg gcaaacgggt tgatgccgtt gtgatgatgg atggtaacgt tggtcagcag
     1561 tactctaccg gctggtttgg cgataacgcc ggtggcgaga actttatgca gttctccgat
     1621 atgtacgtta ccaccaaagg tttcctgccc tttgcgccag aggctgattt ctgggtgggt
     1681 aaacacggtg cgccgaaaat tgaaatccag atgctggact ggaaaacgca gcgtactgat
     1741 gccgcagcgg gtgtaggtct ggaaaactgg aaagtcggtc cgggtaaaat tgatatcgcg
     1801 ctggttcgcg aagatgtcga tgattacgat cgcagcctgc aaaacaaaca gcagattaat
     1861 accaatacta ttgatttacg ctataaagat atcccgttat gggataaagc gaccttaatg
     1921 gtgagtggtc gttatgtcac ggcaaacgaa agcgcatcgg aaaaagataa tcaggataat
     1981 aacgggtatt atgactggaa agatacctgg atgttcggca catctttaac gcagaaattt
     2041 gataaaggtg gcttcaacga attctccttc ctggttgcga ataactctat cgccagtaac
     2101 tttggccgtt atgctggcgc aagtccattt accaccttta atggtcgtta ttatggtgat
     2161 cacaccggcg gaacagcagt tcgtctgacc tcgcagggcg aagcctatat cggcgatcat
     2221 tttattgtgg ctaacgcgat tgtttactcc ttcggtaacg atatttatag ctacgaaaca
     2281 ggcgcacact ctgatttcga atctattcgt gcggttgttc gcccggccta tatttgggac
     2341 caatataacc agacaggtgt tgaactgggc tatttcaccc agcaaaacaa agatgcgaat
     2401 agtaataaat ttaatgagtc tggttataaa accacgctct tccatacctt taaagtcaat
     2461 accagtatgt tgacctcgcg tccggaaatt cgtttctacg ccacgtatat caaagctctg
     2521 gaaaacgaac tggatggctt cacctttgaa gacaataaag acgaccagtt tgctgtcggt
     2581 gcgcaggctg aaatctggtg gtaatggaac ttaatgaaag gaatattacg atgaatataa
     2641 aaattgctgc tttaacgctg gctattgcca gcggtatttc tgctcagtgg gccatagcag
     2701 cggatatgcc agccagcccg gcacccacta ttccggttaa acagtatgtg actcaggtca
     2761 atgccgataa cagcgtgacc tttcgctact ttgcccctgg ggcaaaaaac gtctcctt
//
LOCUS       DAJTVE010000267         2739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000267 DAJTVE010000000
VERSION     DAJTVE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..75
                     /locus_tag="OO439_004248"
     CDS             <1..75
                     /locus_tag="OO439_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6318445.1"
                     /translation="LDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(76..450)
                     /locus_tag="OO439_004249"
     CDS             complement(76..450)
                     /locus_tag="OO439_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6318446.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(533..1579)
                     /gene="mdtG"
                     /locus_tag="OO439_004250"
                     /pseudo
     CDS             complement(533..1579)
                     /gene="mdtG"
                     /locus_tag="OO439_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(1910..2485)
                     /locus_tag="OO439_004251"
     CDS             complement(1910..2485)
                     /locus_tag="OO439_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318447.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          719 a          654 c          631 g          735 t
ORIGIN      
        1 cttgatctgc cattctccct ggtttttgat actttactgc tgccgatcga cactcatcat
       61 ggcccgtatg agtgattaac gttcatccca ctcatcagct gcagcgtgtc cttcctcggt
      121 atccagcggt ggctcaagct gaaattcccc ctcgtcccat tcatgtaatg tattctcttc
      181 ctgccactcc tggcggatct ctatctcatc atagtcgcca tcaaaaacac tttgcgcggc
      241 ttcaccgcta agcataggta aacattcacc ttcttcccct tcgtcggcaa aaaactcaac
      301 ttgccacatg atgtcgccgt cctgcaaaac gtatttttgg gcattgaact gttgcacatt
      361 cgcatcttcg gcgtcgatgc cggggttgtc tgcaagaaat tcttcgcgtg cagcgtcaat
      421 ggcttcttca agcgttgcgt acatggtcat cacgattctc cctttgagtt gatgaggttt
      481 cagggaaaag gatagctgat tctccgcttt tgcaagtatg aaaggcgaaa aatcagttcg
      541 atacctgggg tattcgacga cgacgtagac tgttccatga atagactgcg ttgaataaca
      601 ctacgccagc ggtgacgaga aataccgctc tgaaaccgta gttcgctgaa atcgctgctc
      661 ccatcaatgg tccggtaacg ttgccaatat cacgaaacga ttggttatag ctgaagatac
      721 gtccggcgat ttggttgctc gagttataaa ctaacagtgt ctgtacggcg gggagtagtg
      781 caccatcggc ggcaccgagc aaaaaacgta aaatcccaag ttgcaatggc gtctgaacgt
      841 aagacattgg gatcaacagc agtacagaaa agatcagcgc cgtaatcagg atcttttcgg
      901 gtccgattcg atcgccaagt ttgccgagtc gtggtgcact tagcagagcc gccacgcctg
      961 gcaccgaggc gatcatgcca ctgataaagg cgacgttact gacgttaccc gccagttcgc
     1021 ggacatacag cgtcagaatg ggggcaattg agcccgtcgc cacctggatg attaacgtag
     1081 tgacaaacag gctgagtacc agtttcgggt ttttaagtga tgtcaccact tcccgcatgt
     1141 gcagcatctc ttttttgctg atcggctgga atttttctct tatgcaaaac agggtgacga
     1201 aaaagcagag tatgagcaca ctggcggtaa taaagaatac cggacgtaag ccgtagctat
     1261 cggcgagcag gccgccagcc attgggccga gcaacgcacc actaacgccg cctgtggaga
     1321 gcgtacccag cgcccagccg cttttattac gcggtacttg tgtggcgata agagcattag
     1381 cgttggggac aaatccgcca agtaacccaa gaagcgcccg caggatcaaa aactgccaga
     1441 tattttgtgc cagccccatc aacaccatca cgatgcccat gccgagggca gagcgtaata
     1501 gcatgagttt tcggccttta cggtcggcga gtccacccca aaacggtgag gcgatggccg
     1561 aaaataaaaa tgtaatgctc atatatgcta tcttaatgca ctacctgata agtgagccta
     1621 tacaggttat tgctgtatgt ttatacagta ttattggtag aagactgtgt agatttggta
     1681 tagagatgaa tagatactat taaggtaact tattgaaatc aataaagatt cttgtgcagt
     1741 ctgaatagat aacttagcgt tatgtctagt cgtttccctc tccattccta aaaagtgctg
     1801 tatactggcc tatacaacgc tataaagacc tctacatagc gccttgatag ctatgtcaca
     1861 ggtacaaaaa agccccctat aaaggaggct gtgtagtcta ttaagggtct tacagggtct
     1921 tggtccagtg tctgctaaca gtccttacag agaggttaag aaccttagct acctgagact
     1981 gagatagtcc cctttccttg tactctaaga ccgctctagt tgtgtcactc gctataggtc
     2041 ttccaagtac cttaccttga actctagcac gctctagacc ctgtttagtg cgctctctga
     2101 tacggtttct ttcaaactct gcaaaggtac taaatagctg tagaaccaac ttaccctctg
     2161 agcttgaaag gtctttaacg ggtaaatcta aacagatgag cttcacaccc ttatcattaa
     2221 gaagggtcac tgtattctgt aggtcgatgt tatcccttcc cagacggtca agtttaagaa
     2281 caacaagggt gtcaccttct tctagcttat ggttgaccag attcgcaaat ccgtccctat
     2341 ccatagctga cactgaacca gagacaacct cctctataac tcgtgactta ggaatatcat
     2401 agccagcctt cttaatggct tgaatctggt tctctgtggt ggaagtctct actgatacac
     2461 ggcagtaagc aaaggttcgt gacatagata tccctctggg tagtcattaa acggttagtt
     2521 tgtcttgtct atagacatta aagcaaatgg tagatttgtt gtctatagct atagtgactc
     2581 ttctatgtag acattaaacg gacgtttctt gtcgtgggtc acatagatac ttttagggct
     2641 tctgtcaagt gatattcgta gattttgcaa gggggaggtg taaggctgaa acagggctac
     2701 taagaacaca tacatctaag gctggaaagt acctgagca
//
LOCUS       DAJTVE010000268         2735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000268 DAJTVE010000000
VERSION     DAJTVE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..810)
                     /gene="curA"
                     /locus_tag="OO439_004252"
     CDS             complement(<1..810)
                     /gene="curA"
                     /locus_tag="OO439_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6318448.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMA"
     gene            991..1509
                     /gene="mddA"
                     /locus_tag="OO439_004253"
     CDS             991..1509
                     /gene="mddA"
                     /locus_tag="OO439_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6318449.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1506..1955
                     /locus_tag="OO439_004254"
     CDS             1506..1955
                     /locus_tag="OO439_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6318450.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1956..2189)
                     /locus_tag="OO439_004255"
     CDS             complement(1956..2189)
                     /locus_tag="OO439_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6318451.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2275..2448)
                     /gene="ortT"
                     /locus_tag="OO439_004256"
     CDS             complement(2275..2448)
                     /gene="ortT"
                     /locus_tag="OO439_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6318452.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2643..>2735
                     /gene="yncL"
                     /locus_tag="OO439_004257"
     CDS             2643..>2735
                     /gene="yncL"
                     /locus_tag="OO439_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6318453.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          654 a          700 c          668 g          713 t
ORIGIN      
        1 tgccatcaac aggggtaaac gatccggacc gggtggtagc tctgtagcgt tatagctgct
       61 cactaagcca caaacgggaa tgcgcgcaga tgtattaagt aatggtagca ctgcatcaaa
      121 taccttaccg cccacgtttt cataatagat atcaatacct tttgggcacg ctttcaccag
      181 ttgttcggca aaatcatccg cgtggtgatc aagacaaaca tcgaagccca gcacctcgat
      241 agcatggcgg catttttccg cgccaccggc aacccccacc accctgcaac ctttaagttt
      301 gccgatttgc cccaccgttg ctcccacagg acctgtcgcc gcagctacca ccaacgtttc
      361 gccctcttta ggctgaccga tatccagcag ccccatataa gcggtaaagc ctggcatccc
      421 cagcacaccc agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc
      481 accactggat atgtcatagt cctgccatcc actgtagccc agcacccagt cgctaggctg
      541 ataatcagga tgattcgact ccacaacacg gctcaccgtg ccgccgacca tcacgccgcc
      601 aatatcaaca ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc
      661 cagggacaaa taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgacatcatc
      721 ttcttcaaga cggaaattct ccggaaccgg tgcgccatgt ggacgcgagg ccagaaccca
      781 acgtcgatta cgctgctttt gttgccccat aatgctctcc tgtgcttttg gcatgaatcc
      841 atcaatagta gcaggctcac gggattggtt ttgtttgcag ccgtcacatc ccctctgcaa
      901 cacgcaataa tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac
      961 atcatcatca ttattttatc gaggccagac atgtccatcc gttttgcccg caaagccgat
     1021 tgtgctgcca ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat
     1081 gaccaaacgg ttgatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt
     1141 tatccggtgc tggtaagtga agaagatggc gtagtgacgg gatatgcctc gtttggcgac
     1201 tggcgtagtt tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat
     1261 catcagggca aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgggat
     1321 tgcgggaagc atgtcatggt cgccgggatc gaatcgcaaa atcaggcctc gctgcatctg
     1381 caccattcgc tgggatttgt cgtcaccgca caaatgccgc aggtgggcac taaatttggg
     1441 cgctggctgg atctgacatt tatgcagttg caactcgacg aacgcactga accggacgcg
     1501 attggatgaa tcagtcgctc acccttgctt ttttgattgc cgccggaatt ggtctggtag
     1561 tccagaacac gctgatggtg cgcatcaccc agacctcctc taccattctc atcgccatgt
     1621 tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggca
     1681 tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata cccggcttgc
     1741 tgggatcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa
     1801 ctattgcagt actggtcgcc agccagctaa ttggcggtct gatgctggat atcttccgca
     1861 gtcatggcgt accactacgg gcgttgttcg gcccaatttg tggcgcaatt ttacttgtgg
     1921 ttggcgcctg gttggtagcc agacgctcat tttaatcaga ggatggtgcc gcctttggtt
     1981 aactgttcgt ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg
     2041 gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc
     2101 agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg
     2161 cgcgcgatga cttcatccag atgcgacata ctttctcctt aaccacttgt gaaatttatc
     2221 gattcatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa
     2281 cagtgaaaac aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc
     2341 gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat
     2401 tgctgccata accgcataaa aaacaagcat atgttgatag agagacatgt acgggcgtcc
     2461 agccagatga ataaaacgcg cgcaaatatg cgtcattttt tgacgtgcat caagtttttt
     2521 caccagtttg agaaaaaatc atcaaacagc aacttgccct tatccatctt cagaatgagt
     2581 attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa
     2641 aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt
     2701 ttactcttat ctctatgaga tttggctggt ttatt
//
LOCUS       DAJTVE010000269         2730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000269 DAJTVE010000000
VERSION     DAJTVE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO439_004258"
     CDS             complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO439_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR6318454.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1323..2375
                     /locus_tag="OO439_004259"
     CDS             1323..2375
                     /locus_tag="OO439_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6318455.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          848 a          592 c          611 g          679 t
ORIGIN      
        1 cgccataaag aaaggaaatg cgctcaacgt tccgattaac gcacctgtga tataaaccct
       61 acggcgacca aaacgatcgg caagccaggc aaaacaggga attgtcaggc agcttaatcc
      121 acctaccagc aaaccaatat taaggaaaag ttcgcgcggt agccccatat tctgggttga
      181 ataattaagt gcaaaggcag taacgatgta catcgtcagc aattcgcaca gtcgtagcgc
      241 aataatcttc aggaaagcac cgggatgtcg taacagcgct tcgataaccg ggatgcgttt
      301 tttcgcggca gcttgataat gttgctgttg ttcaaattcc gcggactcct ccatgccatt
      361 gcgcacccac aatgctccca gtaccagtac gatgctaaac aggaaaggaa tgcgccagcc
      421 ccagcttaaa aactgttcgt cagtcgtcat catactgatc aatgaaacca gtccggttga
      481 aagcagtaaa cctacaccgt agccaacttg tacaccgcta ctgtaaaagg cttttttatt
      541 tttcggtgca ctttcaacgg aaagcaacgc cgcgcctccc cattcgccgc cgacggcaaa
      601 tccctgaatg acacgcagtg tcaccagtaa aataggtgcc caccacccaa tggtcgagaa
      661 tgaaggaaga ataccaatca aggctgtcgc gatgcccatc atccagacgg tcagcattaa
      721 catgcgctta cgtcccagtc ggtcgccaaa gtgaccgaaa atgacaccgc cgagcggacg
      781 gaaaaggaat ccgacgccaa aggtagcaaa tgcggcgagc gttcccatcg ccgggcttac
      841 ttgcgggaaa aactcgcgat taaacaccag tgcggcggtg atgccataga gtaaaaaatc
      901 ataccagtcg acgacggcac cagcgaagct gcctaacgca gctcgtcggg cgcgacttaa
      961 cgaaagcgtc ccttcatcgg gacgagtgga gatgagcgtg gagtccataa tgatcctgtt
     1021 tgtactgtct ttttataatt agtatggaaa aggtaactca cattgccgtc gatctataaa
     1081 cgaaccttat gagactacct cgacagagcg catctgcaaa tgattttatg gctgattagc
     1141 ggcaacatga aaaattcgat ggaagatgaa agcgcaggag ttgctggtgt tgtttaagga
     1201 ttaagcagag gacccacagg ttagagatat gaaacataaa agtccgcata tgcacaaaat
     1261 ggcaccctga tgagtctgat agactgcatt gcattataac cagtagggag gcgaggttag
     1321 gtatgttttt agcatcattg ttgagacgta ttgcatttag ttactacgat tataaagctt
     1381 ataatttcaa tattgaaaaa acagactttg ttgtcatcca tattcccgat cagattggcg
     1441 atgctatggc catctttcct gttattcggg cgcttgaatt gcataaaatt aagcatcttt
     1501 taattgtaac gtcgacaatt aatttagaag tctttaatgc gcttaaactt gaacagacta
     1561 aattaacatt agtcacaatg actatgcagg atcacgcaac attaaaagaa ataaaagatt
     1621 tagcaaagaa cataacacag caatacggta cgccggatct ttgtattgag gggatgcgta
     1681 aaaagaacct gaaaacgatg ttatttatca gtcagttgaa agcaaaaacg aattttcagg
     1741 ttgttggtat aaccatgaat tgctactccc ctttgtgcaa gaacgcgtcc cgtatggatc
     1801 agaaactccg ggctcccgta cctatgacat gggcatttat gatgcgtgag gcgggttttc
     1861 cagcagtcag gccaatatat gaattgccac taagtgagga tgtactcgat gaggtgcgcg
     1921 aggaaatgcg atcgttagga tcttacattg cgttcaattt agaaggtagc tcgcaggaac
     1981 gtacattttc attatcgatt gcagaaaatc taatagcaaa aattcaaagt gaaacagata
     2041 taccaatagt gatcgttcat ggacccaaag gtgaagataa agccagggca ttagtggatt
     2101 gttataataa tgtctaccgt ttatccttac caccctcgat taaacgttca gcagcaatca
     2161 taaaagatgc ttatatcgca ataactcctg acacctcaat attacatatg gcaagtgcct
     2221 ataatacccc tgttgttgca atttatgctg attacaaaac gcgatggccc gcaatggccg
     2281 atgtctcgga gtcagtcgtc gttgggcaaa aaattgacaa tataagtctg gatgaattcg
     2341 caaaggcatt aaaaagtgtt ttggcgagaa tatgagacag tacttatgca tagtaatgcc
     2401 gatctgttat gaacggtggt gtcatcaata ctctgaggct ggtcaatcat cacagaaaac
     2461 cagcctttga atcaaagcag tactcaccgg atacgatcat agtcacatcg aacgcaattc
     2521 tggcggtgcc gcatttttat ttattcattt tctgtgagag aagggcaagc tctatagagg
     2581 gaatgtaaaa aagagagctc cgaatggagc actctttaag aaattataac ggaaaatcag
     2641 aggtttttat aacatgtcgc ataagcaaac ccaagctttt caaatacgct ttgatcacct
     2701 gttgccaggg aaactacgta ttgttcgctt
//
LOCUS       DAJTVE010000270         2725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000270 DAJTVE010000000
VERSION     DAJTVE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..2548
                     /locus_tag="OO439_004260"
     CDS             32..2548
                     /locus_tag="OO439_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6318456.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2559..>2725
                     /locus_tag="OO439_004261"
     CDS             2559..>2725
                     /locus_tag="OO439_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318457.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          780 a          657 c          630 g          658 t
ORIGIN      
        1 aacaaatata aaaaacacag gtcatcaggg aatgccacaa cgacaccacc agggacataa
       61 acgcacaccg aaacagttgg cgctcatcat caaacgttgt ttgccgatgg tgctcactgg
      121 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
      181 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
      241 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
      301 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
      361 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
      421 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gcagctgaat ttgatttcaa
      481 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
      541 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
      601 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
      661 aaatggtgct aattttggcc cctggcgatt acgcaactat tccacatgga cacgcaacga
      721 tcagacatca agctggaata ccatcagtag ttatttacaa cgtgatatca aggcattgaa
      781 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacacctt
      841 tactggcgtt caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
      901 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
      961 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     1021 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacgc aacgtcgctt
     1081 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     1141 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaagaacccg aatttgctga
     1201 agccacggca atatatggtt tgaataatac ttttacgctg tatggtggcc tgctcggttc
     1261 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     1321 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     1381 tcaatggcgt acgcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgtcag
     1441 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgtaattg
     1501 gaactataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga
     1561 tggggtaagt ctgtatgcct ccggttcgca gcaagactat tggggcaata acgataaaaa
     1621 caggaatatc tcagttgggg tttccggtca gcaatgggga attggttaca gcctgaatta
     1681 tcaatacagt cgctacacgg atcaaaataa tgaccgcgca ctctctttga atctcagtat
     1741 tccgttagaa cgctggttac cgcgtagccg ggtttcctat cagatgacca gccagaaaga
     1801 tcgcccaacc caacatgaaa tgcgtcttaa tggctcactg ctggatgatg gtcgcctgag
     1861 ctatagcctg gaacaaagtc tggatgacga taacaaccat aacagtagcc tgaacgccag
     1921 ttaccgttca ccttatggca ccttcagtgc cggatacagc tacggtaatg acagcagcca
     1981 atacaattac ggcgttaccg gcggcgtggt tatccatact catggcgtga cgctctcgca
     2041 atatctgggc aacgcttttg cgcttatcga tgctaatggg gcttctggcg tgaggataca
     2101 aaactatccg gggattgcta ctgatccctt tggctatgca gtggttcctt atctcacgac
     2161 ttaccaggaa aaccgtctct cggtagatac tacgcagctg cccgataacg tcgatcttga
     2221 gcaaacaaca cagtttgtgg tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc
     2281 caatatcggt tatcgcgtac ttgttatagt cagcgatcgc aacggtaaac cgttgccctt
     2341 tggcgctctt gccagcaacg atgagacggg gcaacaaagt atcgtcgatg agggcggcat
     2401 actatatctc tctgggatat cgagtaaatc acaaagctgg actgtacgct ggggaaatca
     2461 ggcagatcaa caatgtcagt ttgcttttag tacaccggat tcagaaccaa caacctctgt
     2521 attacaaggc acggcgcagt gccattaagg ataaaaaaat gaaaagagcg cctcttataa
     2581 caggactttt gttgatatcc acatcctgcg cttatgcctc ctcaggaggg tgtggagctg
     2641 acagcactag cggtgcgaca aattacagca gtgtggttga tgatgttacg gtgaaccaga
     2701 cagataacgt gacaggacgg gagtt
//
LOCUS       DAJTVE010000271         2715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000271 DAJTVE010000000
VERSION     DAJTVE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(413..670)
                     /locus_tag="OO439_004262"
     CDS             complement(413..670)
                     /locus_tag="OO439_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318458.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(667..1164)
                     /locus_tag="OO439_004263"
     CDS             complement(667..1164)
                     /locus_tag="OO439_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6318459.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1366..1824)
                     /locus_tag="OO439_004264"
     CDS             complement(1366..1824)
                     /locus_tag="OO439_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6318460.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1821..2318)
                     /locus_tag="OO439_004265"
     CDS             complement(1821..2318)
                     /locus_tag="OO439_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6318461.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2318..2533)
                     /gene="essD"
                     /locus_tag="OO439_004266"
     CDS             complement(2318..2533)
                     /gene="essD"
                     /locus_tag="OO439_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6318462.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          644 a          703 c          618 g          750 t
ORIGIN      
        1 tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa gttatcccgt
       61 gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg atggcatcac
      121 ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt gaaaccttct
      181 gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc atcagtccgc
      241 caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg gctgccggtg
      301 ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact ggtggcccag
      361 aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat ggctacttcg
      421 cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt gccatgtcca
      481 tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga ttgacacagc
      541 cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc atcatgccct
      601 tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat tcaataacat
      661 gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca gggagttaat
      721 ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc cgcagaagct
      781 cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg cacttaagct
      841 gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg ccgcgtactt
      901 gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc catatgttcc
      961 accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca gctcggctaa
     1021 gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt cacttttacc
     1081 actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac gaattggttg
     1141 attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa cctttgccgc
     1201 acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa ggcccattcc
     1261 aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg caaaaagccc
     1321 cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt tcaggcattg
     1381 cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga ttccacttcg
     1441 gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc catcgcccat
     1501 gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc gcttacgccc
     1561 agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa gctcctttat
     1621 gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag cgatggtggc
     1681 gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg cgattgcgtt
     1741 atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa ccagagcgga
     1801 gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg ctcaatctca
     1861 cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg ccgtagctga
     1921 tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga tgttctgaaa
     1981 ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt ttccggtata
     2041 tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt attcaggagg
     2101 gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc tgtatgcccg
     2161 taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac accttccaga
     2221 ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc cccgccacca
     2281 atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc gcagccttgc
     2341 gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg ccaaatacca
     2401 ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct agcagctgta
     2461 aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt gttaacttat
     2521 ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg gaatactacg
     2581 aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag ctaatatcga
     2641 gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac gaagtaacca
     2701 ctcttaacag cataa
//
LOCUS       DAJTVE010000272         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000272 DAJTVE010000000
VERSION     DAJTVE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..769
                     /locus_tag="OO439_004267"
     CDS             230..769
                     /locus_tag="OO439_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6318463.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO439_004268"
     CDS             complement(995..1318)
                     /locus_tag="OO439_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6318464.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO439_004269"
     CDS             1604..1798
                     /locus_tag="OO439_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6318465.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTVE010000273         2658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000273 DAJTVE010000000
VERSION     DAJTVE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2658
                     /gene="lacZ"
                     /locus_tag="OO439_004270"
     CDS             <1..>2658
                     /gene="lacZ"
                     /locus_tag="OO439_004270"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCR6318466.1"
                     /translation="EGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRA
                     GKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPSTQISDFHVATHFNDDFSR
                     AVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNV
                     ENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRG
                     VNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRFGLYVVD
                     EANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHD
                     ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP
                     GETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPW
                     SAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIELPELPQPESAGQLWLTVHVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQLTTSETDFCIELGNKRWQFN
                     RQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQ
                     AEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASD
                     TPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPS
                     ENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHM
                     GIGGDDSWSPSVSAEFQLSAGSYHYQLVWC"
BASE COUNT          591 a          722 c          777 g          568 t
ORIGIN      
        1 gaaggccaga cgcgaattat ttttgatggt gttaactcgg cgtttcatct gtggtgcaac
       61 gggcgctggg tcggttacgg ccaggacagc cgtttgccgt ctgaatttga cctgagcgca
      121 tttttacgcg ccggaaaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggtagt
      181 tatctggaag atcaggatat gtggcgaatg agcggcattt tccgtgacgt ctcgttgctg
      241 cacaaaccga gcacacaaat cagcgatttc catgttgcca ctcactttaa tgatgatttc
      301 agccgcgcgg tactggaggc agaagttcag atgtacggcg agctgcgcga tgagctgcgg
      361 gtgacggttt ctttgtggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc
      421 ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacgctacg tctgaacgtc
      481 gaaaacccgg cgctgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg
      541 cacaccgacg acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg
      601 cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg cggcgttaac
      661 cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat
      721 atcctgctga tgaagcagaa caactttaac gccgtgcgtt gttcgcatta tccgaaccat
      781 ccgctgtggt acacgctgtg cgaccgcttc ggcttgtacg tggtggatga agccaatatt
      841 gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gttacccgcg
      901 atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc
      961 tggtcgctgg ggaatgagtc aggccacggc gctaatcacg acgcactcta tcgctggatc
     1021 aaatctgtcg atccatcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc
     1081 accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg
     1141 ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt
     1201 tgcgaatacg cccacgcgat gggtaacagt cttggcggct tcgctaaata ctggcaggcg
     1261 tttcgtcagt acccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg
     1321 attaaatatg atgaaaatgg caatccgtgg tcggcttacg gcggtgattt tggcgataca
     1381 ccgaacgatc gccagttctg catgaacggt ctggtctttg ccgaccgcac gccgcatcca
     1441 gcgctgacag aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc
     1501 atcgaagtga ccagcgaata cctgttccgt catagcgata acgagcttct gcactggtcg
     1561 gtggcgttgg atggcaagcc gctggcaagc ggtgaaatgc ctctggatgt tgctccacaa
     1621 gataaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg acaactctgg
     1681 cttacggtac acgtagtgca accgaacgcg accgcatggt cagaagccgg acacatcagc
     1741 gcctggcagc agtggcgtct ggcggaaaac ctcagcgtga cactccccgc cgcgccccac
     1801 gccatcccgc aactgaccac cagcgaaacg gatttttgca tcgagctggg taataaacgt
     1861 tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcgc tgaaaaacaa
     1921 ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta
     1981 agtgaagcga cccgcattga cccgaacgcc tgggtcgaac gctggaaggc ggcgggccat
     2041 taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgccga cgcggtgctg
     2101 attacgaccg ctcacgcgtg gcagcatcag ggaaaaacct tatttatcag ccggaaaacc
     2161 taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt agcgagcgat
     2221 acaccgcatc cggctcggat tggcctgacc tgccagctgg cgcaggtagc agaacgggta
     2281 aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt
     2341 gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac
     2401 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
     2461 cagttcaata tcagtcgcta cagccaacaa caactgatgg aaaccagcca tcgccatctg
     2521 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc
     2581 gacgactcct ggagcccgtc agtttcagcg gaattccagc ttagcgccgg tagctaccat
     2641 taccagttgg tctggtgt
//
LOCUS       DAJTVE010000274         2621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000274 DAJTVE010000000
VERSION     DAJTVE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..642)
                     /gene="ydcO"
                     /locus_tag="OO439_004271"
     CDS             complement(<1..642)
                     /gene="ydcO"
                     /locus_tag="OO439_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6318467.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTY"
     gene            695..1231
                     /gene="sutR"
                     /locus_tag="OO439_004272"
     CDS             695..1231
                     /gene="sutR"
                     /locus_tag="OO439_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6318468.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1304..>2621
                     /gene="rlhA"
                     /locus_tag="OO439_004273"
     CDS             1304..>2621
                     /gene="rlhA"
                     /locus_tag="OO439_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6318469.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHT"
BASE COUNT          666 a          662 c          674 g          619 t
ORIGIN      
        1 ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac cttgcgcgat
       61 aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg caacggcctt
      121 ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt ccagactggc
      181 aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg caagcgagtg
      241 cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga ctattaacgc
      301 gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca atccggtgac
      361 caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat accataatgt
      421 cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc cagagatttg
      481 tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg aactggcgta
      541 gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag aaaacagacg
      601 catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt tataacgcac
      661 aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc gctttttatc
      721 cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac tggcagaagc
      781 gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca gtccgacggt
      841 cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat ttatttctcc
      901 gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg tgatcacttc
      961 gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga tggcatctgg
     1021 tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg tcgtcattga
     1081 tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg gggaaggcgt
     1141 tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa ccgtacattt
     1201 tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca acgtcgcgct
     1261 tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg tatcttctca
     1321 tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag ctattttgca
     1381 cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata atgccagtaa
     1441 tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg caaaaatttt
     1501 cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac ggctgattac
     1561 tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga ttctggaact
     1621 tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta cagttgaaaa
     1681 agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag agctgaatct
     1741 tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct ttattcatgg
     1801 ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa cagggcgtag
     1861 cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga aagacgatca
     1921 ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg atcagactgc
     1981 caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag ggcgttacaa
     2041 agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg atgccattat
     2101 tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt tctttgttcc
     2161 atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg cccgtaaagg
     2221 cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg aagtattgaa
     2281 agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg gtgatggcct
     2341 gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg agaaaaccgg
     2401 agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca aaattcgtcc
     2461 acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga caaaaacctc
     2521 cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac aactgattct
     2581 gaccctcacc agtgaagagg gtgtcagcat cacgcatacg c
//
LOCUS       DAJTVE010000275         2536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000275 DAJTVE010000000
VERSION     DAJTVE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..356
                     /locus_tag="OO439_004274"
     CDS             <1..356
                     /locus_tag="OO439_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6318470.1"
                     /translation="GYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKI
                     SLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKP
                     FFTNFNTIERIENYL"
     gene            353..832
                     /locus_tag="OO439_004275"
     CDS             353..832
                     /locus_tag="OO439_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6318471.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(886..1836)
                     /gene="corA"
                     /locus_tag="OO439_004276"
     CDS             complement(886..1836)
                     /gene="corA"
                     /locus_tag="OO439_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6318472.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2206..>2536
                     /locus_tag="OO439_004277"
     CDS             2206..>2536
                     /locus_tag="OO439_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318473.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          737 a          581 c          554 g          664 t
ORIGIN      
        1 caggatacca acctcttatc ctgcaaagca ttaaaaccaa attactttca tcaagactaa
       61 ataaatttaa tgacttcgag gacagtttta atggcttagg aatcagtgtt catgatattt
      121 cagctcaaaa aatatctctt ttaagttttc aaaaatacgc tataggatgg agtgcaacaa
      181 ttcattttgt agcacaggac cacttcggtc ttgatgtaac tgatatcaaa aacaagactt
      241 acagcaaata tagatttttt cgcatatggt tctttttaca acggcataaa gactttgctt
      301 tcaaaccttt tttcaccaat tttaacacta tcgaaagaat cgaaaattat ctatgaaaag
      361 caaactccag aaaattatat tatgtttatt tcttctatgc tgcatctata atttatggac
      421 attgcgtcct gtacagattt tgtatacata ctctgatgct ggcaattcag tctttctggt
      481 cgttgatcat ctgccatgga ctgacagcga taaaataaat tggtatctga agcatcagaa
      541 tgaaattaaa aatcaacatc ccctaccaga aggtagttgg catacctggt atgtcattga
      601 tatcggtaat ggcttcactg actataaaaa atatatcgaa gggccgtatg aagatctcta
      661 ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg aaaaattact taatggttat
      721 taatgaatac ccttatcgta acactcatat tggtattaac gattttactg agtatcaatt
      781 aacacaagaa aataaaatag agcgagtttt caatccgcat gactttaaat aagacaattt
      841 ttagaaataa gccggataaa aaaatagctt tatccggcac tcatattaca gccagttctt
      901 gcgcttaaag tacagatacg gtgccaggcc cgcgaggatc ataaagataa tcgcgcctgg
      961 gtagccgaaa ctccacttca gttctggcat aaactcaaag ttcatcccat agctggaagc
     1021 aacgagcgtc ggcggcagga ataccacgga taccaccgag aagattttga tgatgcggtt
     1081 ctgctcgatg ttgataaaac ccatcgccgc ctgcatcagg aagttcacct tctggaacag
     1141 ggattcgtta tgcggcagca gggattcgat atctcgcagg atttcacgcg cctgctccag
     1201 ttgcccaccc ggtaaacgcg ctttacgcac caggaagttg agcgcgcgct gggtatccat
     1261 cagacacagg cgaactttcc agccgatatc ttccagttcc gccagagtgg agagcgcctc
     1321 gtcgtactca tcgccctgat gcccttccat aatcacccgg ctcaactgct ccaggtcgct
     1381 atagatattt tcaatttcat ctgccaactg ttcgattttg gtttcgaaca gatccagcag
     1441 cagctcgtag gcgttaccgt ctaccatcga ctggctacgg gcacgcatac gatacagacg
     1501 aaaagcgggc agttcacgct cacgcagagt aaacagacga ccatcacgga tggtaaatgc
     1561 cacagtggag ttaccggcgt gatcttccgc atcttcaaag aagaagaagg agtgaatatg
     1621 caggccgtcg tcgtcttcaa agaaacgtgc cgatgcttcg atgtcttcca gttccgggcg
     1681 ggttgccagg ctctggccaa gttcagattg tacgcgcagt cgctcgtcgt cgtccggttc
     1741 gacaagatca atccatactg catttacaag gggttgtgac tcttcgactt ccagccgggt
     1801 cagtcggtta ttttccagtt gaaatgcgct cagcatgacc gggactccca atgcttaaaa
     1861 tatcggacag ttcggtgggc acacagaaac aaattgggtt tcagaccatt aaacagcctg
     1921 actcagcgcg acgggaaaaa ttgaggtcgc tgacaaccgc taaggctatc agcaaaaagg
     1981 gatagcctta ggagttgatc ctggatgaca ggatagtgag ccagtatcta ctgggtgtgt
     2041 ccaaggcgaa tgtcctctta gagtgatcgt gcgcgcatgt tacgccagca aaattttgcc
     2101 gtcaacacgc aacagaacac cacagaacaa tagttgaact tcgcgctttt tggtactcgt
     2161 caaggtcgct aacaggataa aaaaatccaa catccaggac ctataatggc gcatcggcta
     2221 ctcattggta aaggaatgat caccttgaat ctcaaacgga tttttctcgc cctcacctta
     2281 cttcccttat ttgctgttgc cgctgatgat tgcgcactct cagatccgat gctgaccgta
     2341 caggcgtata ccgttaatcc tcaaacagag cgggtgaaaa tgtactggca aaaagccaat
     2401 ggcgaagcgt ggggaacttt acatgctctg ctggtggata tgaatagtca gggtcaggtg
     2461 cagatggcga tgaacggcgg catctatgat gaaagctatg cgccgctcgg tttgtacatc
     2521 gaaaacggtc agcaga
//
LOCUS       DAJTVE010000276         2462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000276 DAJTVE010000000
VERSION     DAJTVE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..713)
                     /locus_tag="OO439_004278"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="OO439_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="OO439_004279"
     CDS             complement(766..1362)
                     /locus_tag="OO439_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318474.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO439_004280"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO439_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6318475.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2462)
                     /locus_tag="OO439_004281"
     CDS             complement(2006..>2462)
                     /locus_tag="OO439_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318476.1"
                     /translation="CNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLV
                     ASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKP
                     NSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          680 a          535 c          492 g          755 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagca gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 ag
//
LOCUS       DAJTVE010000277         2403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000277 DAJTVE010000000
VERSION     DAJTVE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            196..1149
                     /locus_tag="OO439_004282"
     CDS             196..1149
                     /locus_tag="OO439_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6318477.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO439_004283"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO439_004283"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6318478.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          664 a          582 c          550 g          607 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcgacaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tga
//
LOCUS       DAJTVE010000278         2394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000278 DAJTVE010000000
VERSION     DAJTVE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            366..632
                     /gene="hipB"
                     /locus_tag="OO439_004284"
     CDS             366..632
                     /gene="hipB"
                     /locus_tag="OO439_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6318479.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            632..1953
                     /gene="hipA"
                     /locus_tag="OO439_004285"
                     /pseudo
     CDS             632..1953
                     /gene="hipA"
                     /locus_tag="OO439_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2137..2238
                     /locus_tag="OO439_004286"
                     /pseudo
     CDS             2137..2238
                     /locus_tag="OO439_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          665 a          503 c          615 g          611 t
ORIGIN      
        1 gcagtggagt agcaacgcgc aggatcgctg gcatcttggc gtgatggcag gctacgccaa
       61 tcagcattag cctgtggggg aatgtcggtg tgcaactagg tgataaaggc tatagcgata
      121 ctcagggcat gctgggagtg aaatatagct ggtaaaccgt ataagccgca tgtcgagatg
      181 gcatgcggct taatattgcc aacttcaaaa ggcttttaaa gcctcccttt tatccgcgat
      241 cgcggatatc gcagcgttta tcccgtacag cggataagat gtgtttccag attgacttta
      301 tcctcactaa aggataaaac tgataatatc cccttaagcg gataaatttg ctgtggacgt
      361 atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat gcaatgaaac
      421 tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt ggcattaagc
      481 aggcgacgat ttccaatttc gaaaacaacc ctgacaatac cacgctgacg acctttttta
      541 agattttaca gtcgcttgaa ctctcaatga cgctatgcga cgcgaaaaat gcctcgccag
      601 aagcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt ggatgaacaa
      661 ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta agtatgcacc
      721 ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat tgcagagggg
      781 gaatatcacc tctgatgccg tatttaactt cttcgataac ctgttacccg atagcccgat
      841 tgtacgtgac cggatcgtta aacgttatca tgccaaatcc agacaaccgt ttgatttgtt
      901 gtcagaaata gggcgagaca gcgttggtgc cgtgacgtta atacccgaag acgaaaccgt
      961 aacgcgtccg ataatggcat gggaaaagct tactgaagcc agacttgaag aagtattaac
     1021 ggcttataaa gcagatatcc cgctaggcat gattagagaa gaaaatgact ttcgcatctc
     1081 ggttgctggc gcacaggaga agacagcact gctcagaata ggcaatgact ggtgcattcc
     1141 gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa tcaggcagtc
     1201 caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc tgctgctggc
     1261 gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa gggtgcgcgc
     1321 gttagcggtc gaacgttttg acaggcgttg gaatactgag cgaacggttt tacttcgctt
     1381 gccacaggag gatatgtgtc agacattcgg tttaccttca tcggtgaaat atgaatcaga
     1441 tggaggccca ggcatcgcgc ggatcatggc gtttttgatg gggtccagcg aggcgctgaa
     1501 agatcgctat gattttatga agtttcaagt gttccagtgg ttgattggcg caacggatgg
     1561 ccatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac tcacgccatt
     1621 ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaataacat cagcgatctc
     1681 aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga taaaatttat
     1741 ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc cggaagtgca gatgcatgaa
     1801 atcctaagtg actttgccag aatgattccg gcagcactgg ataacgtgaa gacttcatta
     1861 ccgacagatt ttccggagaa cgtggtgacg gcagttgaaa ccaatgtgtt gaggttgcat
     1921 ggacggttaa gccgagaata cggtagtaag tgagatatgg ggtttggtcg ttgttaatga
     1981 gcaatcatga ccggccaacg actggattct cccttggttt atgtttgtgc gcataatgta
     2041 tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg ttattcgttc
     2101 agattaggtg tccttgtgat ttaaagatgt tatcgtggca cgaatatact gccattccag
     2161 gctcgttata ttgctctcgg gcagtccgta gctggtactg caaacgctga tgcgaccttc
     2221 aaagttcaat atctataatt caatcttaag gtggctatac gtcatctgaa attgaaacag
     2281 ggtgtgtgtg gcatggctac gcacacccga gcaattcacc aatgtgaatc tcattattaa
     2341 tgaggagtgg tttgtatttg gagcgctatg aaaaatcaca taatatctaa acta
//
LOCUS       DAJTVE010000279         2283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000279 DAJTVE010000000
VERSION     DAJTVE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..319)
                     /gene="rraB"
                     /locus_tag="OO439_004287"
     CDS             complement(<1..319)
                     /gene="rraB"
                     /locus_tag="OO439_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6318480.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDV"
     gene            481..1485
                     /gene="argF"
                     /locus_tag="OO439_004288"
     CDS             481..1485
                     /gene="argF"
                     /locus_tag="OO439_004288"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6318481.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1557..>2283)
                     /locus_tag="OO439_004289"
     CDS             complement(1557..>2283)
                     /locus_tag="OO439_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6318482.1"
                     /translation="TFLKSPYINDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFS
                     CDQSKDFSCDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKS
                     RLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLN
                     ELGYVYGDEEKTKEIVNKLPGYVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINE
                     EQLGKLNPPEDLRIAIKKLGWNN"
BASE COUNT          647 a          519 c          460 g          657 t
ORIGIN      
        1 caacgtcaaa tttctccgcc agcgtcatca gttgttcaac ctgggcatcg atcagatcgg
       61 cattcaacgc gcactcgctg aggatgtcgc agcaaatcac gatatcacca tcttcaactt
      121 ccagctcttc tggatcggtc acttcgtaac cgagtttaaa cgcttcaact gccgcttttt
      181 ccagagtttc cagatcgtct gcggaaagat ggtgttcgat ggtgtacaac gcgtccggat
      241 cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt ttcttcacgc tgttcttcca
      301 gttgttccgg gtttgccatg gctcattcct cttaatgtcc tgccgatact cttattgtca
      361 cacaccactg acattgcctc cacctttgtg ataaagattt atgctttaga cttgcaaatg
      421 aataatcatc catataaatt gaattttaat tcattaaggc gttagccaca ggagggacct
      481 atgtccgggt tttatcataa gcatttcctg aaattactcg atttcacgcc agctgaactc
      541 aacagcctgc tgcagttagc cgcgaagctg aaagccgata agaaaagcgg taaagaagaa
      601 gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa aagactcgac ccgtacccga
      661 tgctctttcg aagttgccgc atatgaccag ggggctcgtg ttacttatct cggcccaagc
      721 ggcagccaaa ttggtcataa agagtcgatt aaagacactg cccgcgtgct tggtcgcatg
      781 tatgacggta ttcagtatcg cggctatggt caggagattg tcgaaacact ggcggaatac
      841 gctggcgtgc cggtatggaa cggcctgacc aatgagtttc accccacgca gctgctggcg
      901 gatctcctca ccatgcagga gcatttgccc ggcaaaacgt tcaacgaaat gacgctagtc
      961 tatgcaggtg acgcacgtaa caacatgggc aattcgatgc tcgaagctgc agcgcttacc
     1021 ggtctggatt tgcgtctggt cgcgccacag gcatgctggc cggaagctgc gctggttacg
     1081 gaatgccgcg ccctggcaca acaaaatggc gggaatatta cgctgacgga agatgtagct
     1141 aaaggagttg aaggtgctga ctttatctat accgatgtat gggtgtcgat gggtgaagca
     1201 aaagagaaat gggctgaacg cattgcattg ctgcgtgatt atcaagtgaa cagcaagatg
     1261 atgcagttga ccggtaaccc ggaggtcaaa ttcctccact gcctgcccgc gtttcatgac
     1321 gaccaaacga cgcttggcaa aaaaatggcg gaagagtttg gcctacatgg cggaatggaa
     1381 gtgactgatg aggtcttcga atctgccgcc agcattgtat ttgatcaggc ggaaaaccgc
     1441 atgcatacca tcaaagcggt gatggtcgcg acgctcagta aataattgat atcgctataa
     1501 agatgccggg aaatacaaaa acatcccggc gtcacattat tttatgcagt gatagttcaa
     1561 ttattccacc ccaatttttt tattgctata cgtaaatctt ccggaggatt taattttcct
     1621 aactgttctt cattaatata tccatatata ttgattttgc cattccggag gattttcatc
     1681 attcttgttc taatttttaa ttcattaccc atcttttctt caaaaatctt aacgacataa
     1741 cccggaagtt tatttacaat ttcttttgtt ttttcttcat caccataaac atatcctaac
     1801 tcattaagat acagctgttc gccctcataa cacttaagta attgatagaa gtaccactta
     1861 gcaaaatcag gttgcttcat gaaaaaatcc ttcaaaaaaa tatcatttac taaaatgctg
     1921 acaggcactt cattaaatag taacttaaca tcatcaaata accttagtat taagcgtgat
     1981 ttttcttgat tgcttaagct aaaatcattg acatatgcaa ccaacttatc ccaaatcaat
     2041 tcctgtaatt tacggctatt tttatagggt tctttatcga gattgaaaaa taaggtctca
     2101 gctattttaa ttttagattg atcgcagcta aaatctttag attgatcgca gctaaaatca
     2161 atatgattta tcacggagca tgcatacttt atcttttcag aatcaccaaa atattgcatt
     2221 cttttattaa aacaccaatc cagtattctt acatcattaa tatatgggga ttttaaaaaa
     2281 gtc
//
LOCUS       DAJTVE010000280         2278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000280 DAJTVE010000000
VERSION     DAJTVE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1334)
                     /locus_tag="OO439_004290"
     CDS             complement(<1..1334)
                     /locus_tag="OO439_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6318483.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="OO439_004291"
     CDS             complement(1380..2138)
                     /locus_tag="OO439_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318484.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..>2278)
                     /locus_tag="OO439_004292"
     CDS             complement(2170..>2278)
                     /locus_tag="OO439_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6318485.1"
                     /translation="PQLTFNGARILELDGRQPADEILQASLRAIHAALS"
BASE COUNT          593 a          639 c          565 g          481 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcaa agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgataa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggc
//
LOCUS       DAJTVE010000281         2252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000281 DAJTVE010000000
VERSION     DAJTVE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..150)
                     /locus_tag="OO439_004293"
                     /pseudo
     CDS             complement(1..150)
                     /locus_tag="OO439_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(149..>2252)
                     /locus_tag="OO439_004294"
     CDS             complement(149..>2252)
                     /locus_tag="OO439_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HCR6318486.1"
                     /translation="LVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNV
                     SREGKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSN
                     GGSSLGYGENGVGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLG
                     DKASGIGTLNLMDATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGI
                     VNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIALNDKSKGDVRV
                     DGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEA
                     AADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVF
                     NAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLT
                     NALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDRTFTLERDNTAALTHAMLQS
                     DSENTTSVNVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGR
                     NYQVNGTGDVLIDVPKPWNDPIANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSLT
                     LRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKL
                     TLAEHGGAYGATADMSAK"
BASE COUNT          523 a          648 c          483 g          598 t
ORIGIN      
        1 gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt gccccccgac
       61 atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg tatactgcgg
      121 ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc gttgcgccat
      181 aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc agcgctttca
      241 gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat ccataatcac
      301 cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct tccacctcgt
      361 cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt tgcgccttca
      421 ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc gtcagagggt
      481 tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg cccgtcccgt
      541 ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc agcgtatcga
      601 cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa atgagcgtac
      661 cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat gtggtatttt
      721 cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt tccagagtga
      781 aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca tgggtaaagc
      841 caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct ttgagcgcat
      901 tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc gtaccggggc
      961 ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta tgagacgcaa
     1021 tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct gcattgaaca
     1081 ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta atcagcatat
     1141 cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca ccttcgccag
     1201 aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc gcctcaccgt
     1261 gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag taaacttctc
     1321 caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta ccagatgtcc
     1381 ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc acgtcgccct
     1441 tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct ccggtggtaa
     1501 tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta gtagatgatg
     1561 tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttaccg gaggcatttc
     1621 ctccgataaa tccatagccc gttgaattaa gggtggcacc attactgaca tttacgatac
     1681 cgctaccaaa attaccgaca ttgataccca cagtatcgaa cgatgatgtc gcatccatca
     1741 ggttcagtgt gccgataccg cttgctttat cgcccaaaaa agtaatattt tgcgatacaa
     1801 acttaccgcc atcgctaata ttgaggtttc cgacgcccgc aacaccaatg gtggtataga
     1861 cattcttatt gacctcccac atcccccccg tggtgatgct gacattcccg acgccgtttt
     1921 ctccataacc gagtgagctt cctccgttgc tgacaacaag cccctcatta ctgatattca
     1981 tttcgccgtg gccgtcataa ccaagattag ttccgagatt agtgataacg gagttcttat
     2041 ccttaacagt aatgttgcct gtgcctgttt ctttcatccc cgcagtgata attcccgaat
     2101 ctactttacc ttcacgcgag acattaagtt caccgtcacc agcatcaccg atatagatat
     2161 agcggaaagc ttcgcctgtt ccgaggaaac tccaatgccc tttatcggtc acgttgacga
     2221 caccgtggga accgtcctga acgcccacca at
//
LOCUS       DAJTVE010000282         2229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000282 DAJTVE010000000
VERSION     DAJTVE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1105..1440)
                     /locus_tag="OO439_004295"
     CDS             complement(1105..1440)
                     /locus_tag="OO439_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6318487.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1569..1916
                     /locus_tag="OO439_004296"
     CDS             1569..1916
                     /locus_tag="OO439_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318488.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1936..>2229)
                     /locus_tag="OO439_004297"
     CDS             complement(1936..>2229)
                     /locus_tag="OO439_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318489.1"
                     /translation="EQRAAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDF
                     VVRLDGSTCLQLWNKEGWVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          682 a          485 c          420 g          642 t
ORIGIN      
        1 cgattgagtg acagttactg aaacaactat ttcgacacaa aatctcgtct attcaacatc
       61 atgcaaattt gtcagtgact gaatttagtg acagaaccct ctggaattct gccgtgtaac
      121 cacttatatg cagacctgcc ccgttgctct gggttttcag gctgcgatga gtgatgagcg
      181 atgatttcca tacagatgat atatcgactg gaattgattg cctgcaatta gatttagttt
      241 tattgtgtgg atcgttttag ttagtgggga tgaatttccg ataaattaac caatggaaag
      301 gagatgatat gaataaaaag ataactggat tattaataat tagtctggcg tcaattttct
      361 taattgattg cacccagtgc cattaacatc tatctacaca aaaatagagg aaaacaattt
      421 tgatatatca aataattaca agcagctcca ttctttttat tgtttggggg attacatcta
      481 taataattag aacaaagaat tatttttcta agaagaaaac agaccttaat ataagctttt
      541 ttatagcctt ccctatttct ataacaatcc ttttattttg ccgaggtata ttataaatat
      601 aacataatac atctatatgg ggtgtacata tccccacata aaaacacata ctctcattaa
      661 cacgggaaga aactatgttt tccaattctc actacatgta cataatcgtt cttgtactat
      721 ggtttagtat tctcaagtac ttacagagaa agaatagcat attacatcct ttattttcac
      781 taatcctgac accactaacc attttaatca ttagacttat gatagattta acatacaacc
      841 aataaattat aaacaaaata aaaataggga acattatatg ctttataaaa ttttcacatt
      901 aaacacaaag ataaaaacaa ctatcattac aatatgtgca ttatcatata atcttacagc
      961 gcaagcttcc ccgtcataca gacctcgtga tcgagttaat aagactatga tagaactaca
     1021 aactaacgga gtagaacggc aacttttaga taattcttgt ggcataagtt ctggggtatt
     1081 taaataaaat ataagacagg ctgtctatct tacagacagc ctttttataa taacagaaag
     1141 aatatatatc atacttaacg gaagagataa tatgaaaaca gtacccgtat aaacagcaag
     1201 caatcccatt tttccaggtg tatcagcaaa aaaaacgctg ttccagaaat caggccgggt
     1261 aaatttcaga gctgtatctt caatatacca ttttgcaacc ggatacaaaa ccattccgca
     1321 gagagaaata caccagaaca gtaatctgta tttaaaatca taatcccatg acatatacag
     1381 catatatcct cccgtcaccc atccccacca catattatta aaataatatt ttctgttcat
     1441 ctgccagttt tcctttcttt tgcacttttg tcagtgtact gatgcatgac aacataacca
     1501 cagcatgata ataataatca ataacaataa gctgtgtcac gtttacatca tggaacggag
     1561 gtgctcctgt gacttttgag cagaaaaaag cccgggccat tgccctgatg gacagcaaaa
     1621 agatgtggcg gagcaattat gcccctcctc ttctgcgtat tctctggcgg ctgggtatcc
     1681 ggcttccgcc attacccttt atgccgttct ggcaggtcac ggtgctgacg gggggcttat
     1741 ggggcatttc ctggggatgt gcaatgtggt ttatctactg ggggccgtca ggaatggtgg
     1801 caggtgaggc cattattata agtatcacgg ggggtttttt gtccggactg ctcatggcct
     1861 ctttccactg gtggcgcagg aaagttaacc gactgccacc atgggatgat gtgtaaaccg
     1921 tgcacaggcg tgttcttacg gagcggcact ttcatttatc tgtacgcgaa cctccattcc
     1981 tgcatcgtga caggccggca gccactgcgc cacttccagc ggatcgccct cccggcgtac
     2041 cacccagcct tctttattcc ataactgcag acaggtgctg ccgtcgagac gcaccacaaa
     2101 atccccacgg caggcctgat acgttatgcc gttcagctcc accgccagat cagtggcaga
     2161 agtgccgcct gcgggcagac ctgctgccgg gaccagcggc agtacgacga cttctgccgc
     2221 acgctgttc
//
LOCUS       DAJTVE010000283         2223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000283 DAJTVE010000000
VERSION     DAJTVE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..709
                     /locus_tag="OO439_004298"
     CDS             <1..709
                     /locus_tag="OO439_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HCR6318490.1"
                     /translation="KTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQ
                     GGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPD
                     ELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHW
                     HVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKA
                     AMDRNYLASVLAGSGLS"
     gene            879..1157
                     /locus_tag="OO439_004299"
     CDS             879..1157
                     /locus_tag="OO439_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6318491.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            1157..1534
                     /locus_tag="OO439_004300"
     CDS             1157..1534
                     /locus_tag="OO439_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318492.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            2003..>2223
                     /locus_tag="OO439_004301"
     CDS             2003..>2223
                     /locus_tag="OO439_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6318493.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDT"
BASE COUNT          753 a          331 c          529 g          610 t
ORIGIN      
        1 caagacggat gagaaatcta atgagattac agctatccca gaacttctta acatgctaga
       61 tattaaagga aaaatcatca caactgatgc gatgggttgc cagaaagata ttgcagagaa
      121 gatacaaaaa cagggaggtg attatttatt cgcggtaaaa ggaaaccagg ggcggctaaa
      181 taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagagc atgacagtta
      241 cgcaatgagt gaaaagagtc acggcagaga agaaatccgt cttcatattg tttgcgatgt
      301 ccctgatgaa cttattgatt tcacgtttga atggaaagga ctgaagaaat tatgcgtggc
      361 agtctccttt cggtcaataa tagcagaaca aaagaaagag ccagaaatga cggtcagata
      421 ttatatcagt tctgctgatt taaccgcaga aaagttcgcc acagcaatcc gaaaccactg
      481 gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag acgactgcaa
      541 aataagaaga ggaaacgccg cagaattatt ttcagggata cggcacatcg ctattaatat
      601 tttaacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa aagcagccat
      661 ggatagaaac tatctcgcgt cagtccttgc ggggagcggg ctttcgtaat ctttccctag
      721 ggagtcagcc gaatgttggg aaggatttga cggatgaaga gaagaactgc ttggccggcg
      781 gcagtacggg aagcccggga ggatggggac cagaagatga gaatagcgca agggatcgta
      841 tgcagcatga acggtataaa gatgaattgc gacagaaaat gggaaaaccc gacgttacag
      901 attcaaattt gaaaaatatt atagacgatc tttatcggcc aaatgcaaaa gtaggaagtg
      961 gaagcacagc agatgctgtt cgttatgaat tggccacagg tgaaaaagtt ggtggtagag
     1021 gacatgtatt gaaagcacag gattatacaa gggctttaca ggattggttg gataagaatc
     1081 caacagcatc tccaggggat cgagcagcag cagaaaacgt tcttcaggat ttacagaatg
     1141 ctttgggagg caagtaatga accaatatat tgctagttat atagaaaaaa tggcatcatt
     1201 ttccactgtt ttgtctgaga cagtctcatt agctaatgaa tactggcatc cagaaacgcc
     1261 accggtggtt atgttgttct ctcaggttgg aaaaacgatt gtaaaaaatt tttatgaaat
     1321 gggggatatt gaaagagaaa aaatatttaa acatgtcgaa gatggtatgg tctcgcttga
     1381 tgatgaattg gccacagttg tagctacagg gcttatagag gcaatagtca ctgcgaccga
     1441 tagcaattct ttactttggc aagatttaag taaatttctg ggtgacaaat caaagtctca
     1501 tgcattagtg tggaaaaatt ttggtcagga gtgatattat tcaagaagta ttaagctcgc
     1561 tattgagatc ttaatgcttc ttgagattaa attaaagtta ggacgaagta attgcaggtg
     1621 gaaataaatc catagtgacg tgtggcaatg gctattagat gttatggtgt gatgggcttt
     1681 ggtgatttaa gagctaagtt agattgcctt atgaaaaagt atggttatat atttaatgat
     1741 gtgtatcata tatttaaata aaaaacggtt ctctcttgtc aagatagtaa taggatagac
     1801 tattgtgttg ataaatataa ttaagagtct actttgttga ttttagaaga ctttggagca
     1861 gggaaagatc atcggaagta cactttttgt actaaataat tcgcgctaaa tgttcaataa
     1921 ttgagatatt ccttattacc taaaactatt tttcatagct tatatatgat caaatactcc
     1981 ttgcataaat aaggagaaca aaatggaact taaaaaactg atggaacata tttctattat
     2041 ccccgattac agacaagcct ggaaagtaga acataaattg tcagatattc tactgttgac
     2101 tatttgtgcc gtcatttctg gtgcagaggg ttgggaagat atagaggatt ttggtgaaac
     2161 acatctcgat tttttgaagc aatatggtga ttttgaaaat ggtattcctg ttcacgatac
     2221 tat
//
LOCUS       DAJTVE010000284         2205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000284 DAJTVE010000000
VERSION     DAJTVE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(15..305)
                     /locus_tag="OO439_004302"
     CDS             complement(15..305)
                     /locus_tag="OO439_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6318494.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(295..732)
                     /gene="ratA"
                     /locus_tag="OO439_004303"
     CDS             complement(295..732)
                     /gene="ratA"
                     /locus_tag="OO439_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6318495.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            903..1385
                     /gene="smpB"
                     /locus_tag="OO439_004304"
     CDS             903..1385
                     /gene="smpB"
                     /locus_tag="OO439_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6318496.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1600..1962
                     /gene="ssrA"
                     /locus_tag="OO439_004305"
     tmRNA           1600..1962
                     /gene="ssrA"
                     /locus_tag="OO439_004305"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          619 a          577 c          492 g          517 t
ORIGIN      
        1 cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc
       61 ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt
      121 agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc
      181 aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc
      241 acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct
      301 agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag acgccagctc
      361 tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg
      421 aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt
      481 cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca actggttgcg
      541 ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc
      601 aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg
      661 aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct
      721 aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca tttatcctgc
      781 tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt
      841 gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc
      901 ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg
      961 cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg
     1021 aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg
     1081 acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg
     1141 tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat
     1201 tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa
     1261 atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt
     1321 cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa aacgcccacc
     1381 gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc
     1441 gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc
     1501 gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc
     1561 atggcgctca taaatctggt atacttacct ttacacattg gggctgattc tggattcgac
     1621 gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa
     1681 aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc
     1741 tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc
     1801 gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg
     1861 tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg
     1921 taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac aggactgcaa
     1981 ccggacagag actataaaaa cagtcactta cggacactga ccagacagta agcggaccag
     2041 aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct ctgattatga
     2101 agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc agcttaccaa
     2161 ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agacc
//
LOCUS       DAJTVE010000285         2183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000285 DAJTVE010000000
VERSION     DAJTVE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..168
                     /locus_tag="OO439_004306"
     tRNA            82..168
                     /locus_tag="OO439_004306"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:116..118,aa:Leu,seq:cag)
     gene            complement(207..443)
                     /locus_tag="OO439_004307"
     CDS             complement(207..443)
                     /locus_tag="OO439_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6318497.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            584..1372
                     /gene="fhuF"
                     /locus_tag="OO439_004308"
     CDS             584..1372
                     /gene="fhuF"
                     /locus_tag="OO439_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6318498.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1410..2087)
                     /gene="bglJ"
                     /locus_tag="OO439_004309"
     CDS             complement(1410..2087)
                     /gene="bglJ"
                     /locus_tag="OO439_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6318499.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(2045..>2183)
                     /locus_tag="OO439_004310"
     CDS             complement(2045..>2183)
                     /locus_tag="OO439_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6318500.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          566 a          573 c          488 g          556 t
ORIGIN      
        1 ttagtgttct tacggacgtg ggggttcaag tctcccccct cgcaccaaac gaggcgatat
       61 caaaaaaagt aagatgactg tgcgaaggtg gcggaattgg tagacgcgct agcttcaggt
      121 gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaat tatctttact
      181 tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat catcagcatg
      241 atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa ctgtttgtga
      301 tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca ctgatccggc
      361 gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac tccccagcca
      421 ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat aaccaatatc
      481 atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg taaacctatc
      541 gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct atcgttccgc
      601 accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg ctgggcttgc
      661 acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt ttatccgcct
      721 ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac cgaatgcgct
      781 aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa cgatgatccg
      841 cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga tggtgccacc
      901 attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg aacatttcca
      961 tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt gtgaagataa
     1021 aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc aggcgctggt
     1081 tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta tctggagtaa
     1141 taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg gcgaggcgac
     1201 ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg gtgaagataa
     1261 tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa cctgctgcca
     1321 gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat gaaagcactg
     1381 ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt acttgcttcg
     1441 caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc agaactgacg
     1501 cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt gcgtttaagc
     1561 tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat ttctctctcc
     1621 gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg gtcggtcgcc
     1681 tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag cgttgacgct
     1741 ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg agcttcaata
     1801 tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag ttcagtcagg
     1861 caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat gaccgccgca
     1921 aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt atggagctta
     1981 taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct catgacgcat
     2041 ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct gcggcatctc
     2101 cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc catgcataca
     2161 tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAJTVE010000286         2181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000286 DAJTVE010000000
VERSION     DAJTVE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..953)
                     /locus_tag="OO439_004311"
                     /pseudo
     CDS             complement(1..953)
                     /locus_tag="OO439_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
     gene            1026..>2181
                     /locus_tag="OO439_004312"
     CDS             join(1026..1275,1275..>2181)
                     /locus_tag="OO439_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6318501.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          566 a          540 c          552 g          523 t
ORIGIN      
        1 tctccccatt cactgtacag tgacccgatg cctcgggaac tactgtggtt taaatagttt
       61 gctctgggga gcgtgtagcc attgctgctg caaaaatttt cagcaaggct gtatatcatg
      121 aaagcctcgc cggcgttcac ccaccaactc ttcacacgga tttcgtatac atagctaggg
      181 cctcctgatt ttggtgtcgc cgtaatcctt tccgaattgc tgccgacatt tttaaatgtc
      241 actttaccgg tagcatcaac atcgacccag gaggcagagc ttgaaaactc ataatcagcc
      301 gccgtttttc ctggggcaaa gttgtcatta ttcaactgat aatacgcccc ggtgaacccc
      361 tgcgaaggga atgtagttgt cggtaggtca gtaccattga ctgatactgt accgctcatt
      421 attttgtctt ctgcgcgagt gaattgtatt gtggtactca gaccagcttg atgaacacca
      481 ttcaatacag ggatcagcgt tgtgataccc tctccgcctc ctgtaacagt ggctttgtac
      541 tcgcctgaga aatttttact gtagtcaatt gcactaactt gcacataggg cacgttggta
      601 cctgactgta caaattccag cccttcagaa actttgatcg gattgcctga tatatcgtgc
      661 agaacaagat gtagctcagc actatcggta aagtctcctt tcaatgatga ccgattgttc
      721 ttaaggactg actttgaggc gtctgctggg cctgccacca gcgttatatc tactgtttgc
      781 attccaccag cggtcgtgac tgcgcttacc gtgacgctac ctgtcatcgc tcctttcacg
      841 cttgttgtcg cgattccgtt ttgatcggtc accgctgtta atgacgttaa tgtggcagag
      901 ccgcttttta aggcaaagta cacagtgaga ccttcgatca ggttaccact gcctgaagtg
      961 gcacactgaa tttggccacc tgaacagagg tgatatgctc acctcagaac aacacaggtg
     1021 ctccaatgaa aaaaagaaat tttagcgcag agtttaaacg cgaatccgct caactggttg
     1081 ttgaccagaa atacacggtg gcagatgccg ccaaagctat ggatgttggc ctttccacaa
     1141 tgacaagatg ggtcaaacaa ctgcgtgatg agcgtcaggg caaaacacca aaagcctctc
     1201 cgataacacc agaacaaatc gaaatacgta agctgaggaa aaagctacaa cgcattgaaa
     1261 tggagaatga aatattaaaa aaggctaccg cgctcttgat gtcagactcc ctgaacagtt
     1321 ctcgataatc gggaaactca gagcgcatta tcctgtggtc acactctgcc atgtgttcgg
     1381 ggttcatcgc agcagctaca gatactggaa aaaccgtcct gaaaaaccag acggcagacg
     1441 ggctgtatta cgcagtcagg tacttgagct acatggcatc agccacggtt cggccggagc
     1501 aagaagcatc gccacaatgg caacccggag aggctaccag atgggacgct ggcttgctgg
     1561 caggctcatg aaagagctgg ggctggtcag ctgtcagcag ccgactcacc ggtataaacg
     1621 tggtggtcat gaacatgttg ctatccctaa ctaccttgaa aggcagttcg ccgtgaccga
     1681 gccaaatcag gtgtggtgcg gtgatgtgac ctatatctgg acgggtaagc gctgggcgta
     1741 cctcgccgtt gttctcgacc tgttcgcaag aaaaccagtg ggctgtgcca tgtcgttctc
     1801 gccggacagc aggctcacca tgaaagcgct ggaaatggca tgggaaaccc gtggtaagcc
     1861 cggcggggtg atgttccaca gcgatcaggg cagtcattat acgagcaggc agttccggca
     1921 gttattgtgg cgataccaga tcagacagag tatgagccgg cgcggaaact gctgggataa
     1981 cagcccaatg gaacgcttct tcaggagtct gaagaacgaa tggatgccgg tggtgggtta
     2041 cgtaagcttc agcgaggcag ctcacgccat aacggactat atcgttggat attacagcgc
     2101 actaagaccg cacgaatata acggtgggtt acccccaaac gaatcggaaa atcgatactg
     2161 gaaaaactct aactcggtgg c
//
LOCUS       DAJTVE010000287         2169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000287 DAJTVE010000000
VERSION     DAJTVE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1248)
                     /gene="phrB"
                     /locus_tag="OO439_004313"
     CDS             complement(<1..1248)
                     /gene="phrB"
                     /locus_tag="OO439_004313"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6318502.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFI"
     gene            complement(1245..1754)
                     /locus_tag="OO439_004314"
     CDS             complement(1245..1754)
                     /locus_tag="OO439_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6318503.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          577 a          541 c          497 g          554 t
ORIGIN      
        1 gataaactca ccttcgagat caaatttctc gccctgggtt gtcgggttga aaatacgaaa
       61 atacggcgct gcatcggttc cggttgaagc ggcccactgc cagccaccgt tattggctgc
      121 caaatcacca tcaatcagct gcgacatgaa atatcgctcg ccttcgcgcc agtcgatcaa
      181 taaatctttc accagaaaac tggctgtaat catccgtagc ctgttatgca tccagccagt
      241 gctgttaagc tgacgcatag cggcatcaac aatcgggtat cccgttttgc cttcctgcca
      301 ggcctgtaaa tgtgcgggat tgctctgcca ctgtacacga tccgtccagg caataattgg
      361 acgatgttta cacaacgagg ggtgatacgt tatcaggtga cggtaaaact cgcgccagat
      421 cagctcatta agccagacac taccgacccc accgtccagc gcctgcggct gttcagccaa
      481 caagcgatgc aagcactggc gaggcgataa cccgcccgtt gccaggctgg ccgacaaacg
      541 gctggtgcct tccactgccg gaaaatctcg ttgttgctca tattctccgg caccgttctg
      601 gcaaaactgg cgtaattgcg caatcgccgc tttttcttcc accggaaaat gcgcagtatc
      661 gaaagactga cgaggataat tcagcgtaat ggatggcgag ggctctatcg atccgctact
      721 acgaactttt ggcgcagcga cgcactccgg catcccttcc cgcagccgtt tcagccaggc
      781 attcttaaaa ggcgtaaaga ctttgtacat ctcgtgatta ccggtcatca ccgcgccagg
      841 cggcaggatc acgctgtcat caaatccttc acacaccacg ttacgcagcg ctctttcaac
      901 ttccacatcc cgcgcccgct cattcacttc atactgatag ttataaaaca ggtgggtaac
      961 gctgttttcc gcgcacacct gtttaactat ttcgacactg gcgacaaagt catccacttc
     1021 acggaacaat aaaggaatac ctttttccgc aagcgctatt tgtagcccat tcagttgagc
     1081 attgataagt tcagcctgac gcggcgacat gttatgcgtc gcccactggc gtggtgtagc
     1141 gatatacaac gccagcacgc gtgcagacga attgcggcag gcggcagcca gtgcgagatt
     1201 atcgtgcaga cgtaaatctt ggcgaaacca gaccagatgg gtagtcataa aactcctgaa
     1261 tggcgcaagt taatcacacg cggccaaagt tcgaaataac gctgccctga aagccaggcg
     1321 tcatgataaa gcgccatata gtgtttgata cgcatcagcg gcgcaagaag tggttgctcg
     1381 ccacggcgat aactgtcgat aagcgcagcc agctgctggc gttctgtgga atcaatatgc
     1441 gggcgaaaat aaccctgaac gtgcatcaaa acattggtgt gatcgcgcgg gttggcgggg
     1501 tgagaaagca aaacaactac gcgttggcgg tactggttgt aatagtcatc cagattttgc
     1561 cactggtgaa tatcagccac gaacgggcca agtttgcggt actccggctg agaatgcgcc
     1621 agaaagacga gtttatagcg gctgtgataa tcgagaagcg ccccgcgcgt taagccatgt
     1681 tctttcagtc gatgtaactc atgaagggca aagatacgga ttagggtgct gtcatcaaat
     1741 cgttgtagat tcattactgc tcctgaacgg tataaaggag taagtgtaga cgatggtaaa
     1801 tgaattttat ggggcagagt ttttgcgggg aagtattttg ttggtaaaaa tattatcaac
     1861 ttcatctgag tgagaattat tctactattt cccgagctgc attcattttt tgtatttttt
     1921 atttttagaa aataacagtt ctcaaagctt aattacctca ctattaattg tatttcaaac
     1981 taacaatcgc actccatttt ctctttttaa aaaacataaa cactgttaaa acaaatactt
     2041 atttcgttat cttaaatgct atttctacat cactcgtcct tataatcaaa ttattcttat
     2101 tcatagctat ttttgcaggt ttaaaatagc ggctttcacc aaagtcagcc atgaaaatta
     2161 gactacaag
//
LOCUS       DAJTVE010000288         2089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000288 DAJTVE010000000
VERSION     DAJTVE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..1025)
                     /locus_tag="OO439_004315"
     CDS             complement(60..1025)
                     /locus_tag="OO439_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6318504.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OO439_004316"
     CDS             complement(1879..>2089)
                     /locus_tag="OO439_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318505.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          550 c          435 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJTVE010000289         2088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000289 DAJTVE010000000
VERSION     DAJTVE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OO439_004317"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OO439_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6318506.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="OO439_004318"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="OO439_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6318507.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAJTVE010000290         2005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000290 DAJTVE010000000
VERSION     DAJTVE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO439_004319"
     CDS             <1..389
                     /locus_tag="OO439_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318508.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO439_004320"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO439_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6318509.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO439_004321"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO439_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6318510.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO439_004322"
     tRNA            complement(1806..1881)
                     /locus_tag="OO439_004322"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="OO439_004323"
     tRNA            complement(1890..1966)
                     /locus_tag="OO439_004323"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAJTVE010000291         1952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000291 DAJTVE010000000
VERSION     DAJTVE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..403
                     /locus_tag="OO439_004324"
     CDS             <1..403
                     /locus_tag="OO439_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6318511.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(398..592)
                     /gene="cas6e"
                     /locus_tag="OO439_004325"
     CDS             complement(398..592)
                     /gene="cas6e"
                     /locus_tag="OO439_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6318512.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGGAPIKLSSML"
     gene            complement(574..1320)
                     /gene="cas5e"
                     /locus_tag="OO439_004326"
     CDS             complement(574..1320)
                     /gene="cas5e"
                     /locus_tag="OO439_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6318513.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(1331..>1952)
                     /gene="cas7e"
                     /locus_tag="OO439_004327"
     CDS             complement(1331..>1952)
                     /gene="cas7e"
                     /locus_tag="OO439_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6318514.1"
                     /translation="EEDLALLRKDRIAVDIAMFGRMLANKPEFNVEAACQVAHAFGVS
                     ETIVEDDFFTAVDDLRQASEDAGAGHLGETGFGSALFYTYICIDKDLLVENLGGDEAL
                     ANQTIRAFTEAALKVSPTGKQNSFASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQ
                     LDVAVQRITTLRENMNTVYEQKTECASFDVMNKQGSMKDVLDFICA"
BASE COUNT          449 a          565 c          490 g          448 t
ORIGIN      
        1 agaacagcga gttcttgctc accctcttta cccggtcctg accagtatgc ccggagtcgg
       61 tgtcaggacc gcagccagac tcctcaccga ggtcgcctgc cgcgccttcg cctctgccgc
      121 acatctcgct gcttatgctg gccttgcgcc ggtaactcgg cgatccggct cgtcaatacg
      181 cggtgagcat ccctcgcgac ggggtaataa agctctcaaa cgggcgttgt tcctgtcggc
      241 cttcgccgcg ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg
      301 aaaacgacac aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc
      361 catgatgcgc gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa
      421 taggggcacc cccctggagt tcttcccgac gataaagaaa ttgcctttct ttgccgccag
      481 ggaaaagatc ccacagccac tgatgcatca catattcacc gcgatccacc agatgcagca
      541 actgcgcagg cgaaagctgg ccggtatgta aggttattct tgagaggtac atggttcctc
      601 cttgctgagc cacggcccct gattgacggt gcgttcccca aacagccact gctgacgatt
      661 taaaggaaca tcccggcggc gtaatatttt gctcgcgacc aggccgtcgt gtttcccttc
      721 ccaccagcat tcatcctgaa gtttcgggag tgagactttc agtttatgga aatgatcctg
      781 atactgttgg tatgcattac ataaggcatc acacgcgttg ccttcgagca gtaacggcgc
      841 aagaggtaat gccagcggat gactttttcg ccccagataa agcggaaaaa ctggatggcg
      901 taaaccgtcc tgcaactgtt cgaggctgta aggcgcatcg gctgttgttg ccaccgccac
      961 catccaccag gcatcggtgt agtagtcgcg ccgggagata atcgcgctca aaagctcagg
     1021 gtcgctcaac tcttcacgac ggctgaaata acgtgcttta cgcacctctt ttggcatctg
     1081 gaccgtgtga taatcccgtg cccagcgcgg gttacggctg gcgcaaacca ccagtgaata
     1141 gtggcgatta aacgcgttta agcgttcggt atcatcacgc cgaatcccta ccccggcagc
     1201 cagtagcccc agcaatgctg agcgcgaagg aagttcatgg gtatgacgaa cttcacctgg
     1261 agcatcgaca ccccaggacg ccatcggccc atgaagctga aaaatcagat attggctcat
     1321 aagccgcccc ttacgcgcag ataaagtcca gcacgtcctt catgcttccc tgtttgttca
     1381 tcacgtcaaa acttgcgcat tcggtcttct gttcgtagac cgtattcatg ttttcgcgaa
     1441 gcgttgtaat acgctgcacc gccacatcta actgacgtgt gccattaatg ggttcataga
     1501 aagccgccgc cagagagcgt ggttgatcgg tgcctttttc tgccagcgcc caggaagcat
     1561 aggcacggct ggcaaagcta ttttgtttac cggttgggga aactttaagc gcggcttccg
     1621 taaaagcgcg tatggtctga ttagctaacg cttcgtcacc gccgaggttt tctaccagca
     1681 gatctttatc aatgcagata taggtgtaga aaagtgctga accgaatccg gtttctccga
     1741 gatgcccggc accggcatct tcagaggcct ggcgcaaatc atcaacggcg gtgaaaaaat
     1801 catcttcgac aatcgtttca ctgacaccaa acgcatgcgc gacctggcag gcggcttcaa
     1861 cattaaactc aggtttattc gccagcatac gaccaaacat agcgatatct accgctatac
     1921 ggtctttacg taacaacgcg agatcttcct ct
//
LOCUS       DAJTVE010000292         1937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000292 DAJTVE010000000
VERSION     DAJTVE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..558)
                     /locus_tag="OO439_004328"
     CDS             complement(<1..558)
                     /locus_tag="OO439_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6318515.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTL"
     gene            651..1034
                     /gene="crcB"
                     /locus_tag="OO439_004329"
     CDS             651..1034
                     /gene="crcB"
                     /locus_tag="OO439_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6318516.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1088..1297)
                     /gene="cspE"
                     /locus_tag="OO439_004330"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1088..1297)
                     /gene="cspE"
                     /locus_tag="OO439_004330"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6318517.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(1472..>1937)
                     /gene="pagP"
                     /locus_tag="OO439_004331"
     CDS             complement(1472..>1937)
                     /gene="pagP"
                     /locus_tag="OO439_004331"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6318518.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          492 a          481 c          441 g          523 t
ORIGIN      
        1 caacgttgac cagtgatgct ctttgagcgg cccgcgaacc caggcggcag gaagtaccag
       61 gatttcagct ccctgtaatg cctgtgccag cgccagctct ggaaagcgta agtcataaca
      121 ggtcatcaga ccgaccttca tcccctccac ctcgagtaac ggagcgattt cattaccagc
      181 atcaacacgg cgtgattcct gaatggcaaa tgcatcatag agatgcaatt tggcataacg
      241 ggcgacgatg tttcctgcct gtagcgccac cagcatattc catgcgcgcc ccggcgttga
      301 aggaacatga atcgtcagaa ttgtcgtcat catgttacgt ttactttctc gccgtaaacg
      361 tccgaggaat tcgccttcca gcagctgtgc tgatttaacc gatagatctg catcatgatc
      421 atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat acgtcgtttt ccgccgcctg
      481 cgccatcaac gaggcacaaa tctcagcgtt cttttcccac acagatgtaa cagcaaactg
      541 tcctgcggca accagcattg ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt
      601 attcggcgtt acacttatca ctcatacaaa tcaaatagca ggattttgca gtgttacaac
      661 ttcttttagc agtttttatt ggcggtggta cgggaagcgt ggcgagatgg ctgttaagta
      721 tgcgatttaa cccgctgcat caggcgattc cgttggggac gctggcagca aacctgattg
      781 gggcattcat cataggaatg ggattcgcct ggttcagcag gatgacgaac attgatccag
      841 tgtggaaagt attaatcacc accggatttt gtggcggtct aacaaccttc tcaacatttt
      901 cggcagaagt ggtgtttttg ttacaagagg gccgctttgg ctgggcgtta ctgaacgttt
      961 tcgtcaacct tctggggtct tttgccatga ccgcactggc tttctggctg ttttcggcct
     1021 caaccgcaca ctaaagacca aaaaaaaccc gctgattaag cgggttttga attcttgctg
     1081 acgtatctta cagagcgatt acgtttgcag cagaagggcc tttggcaccg ttagtgattt
     1141 cgaactctac gcgctgacct tcagcaagag ttttaaaacc attagtctgt attgcagaga
     1201 agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat cctttggact
     1261 cattaaacca cttaacgtta cctttaatct tagacatcaa aattaccttt acatgaaaaa
     1321 tagacacaaa tgctgtgtcg gttaccagta caccaattgt ggtacgcttt gtccagtgca
     1381 actttactaa aaagtgataa aagtcgcgtt atttttgatg ttacttattt tagttattga
     1441 ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg aaagcgcatc caggcaaagt
     1501 acacattgcc attgttgtag gtacccggaa tgtaggtcat ctgaaaagtc actgggccat
     1561 aacccacgga ggccaatggc agtagaaccg ggagagggat gtaattccag ttatcgcgtg
     1621 ccgttacgcc agcggtgaat cccagaccta aatgaaaatt ttcatccgcc agcggtcgcc
     1681 aggtactttc ccatccgtat ccggcaatcg gttcccattt gttccacgag tccttaaatg
     1741 ccatggcata caggccatgc cagtttcctt tttcatccca tcgcgactga ccaaaaccgc
     1801 caccccacgg tcgctcgtta tagcgatcgg ttttttcttt gtcgtaagcg aaacgtgcat
     1861 gccaggtgat ggcaggaata tataaatcat aatgttcagg ctgttgccag gtttgtgcaa
     1921 tattttctct aaacgtt
//
LOCUS       DAJTVE010000293         1885 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000293 DAJTVE010000000
VERSION     DAJTVE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..345
                     /locus_tag="OO439_004332"
     CDS             76..345
                     /locus_tag="OO439_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6318519.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            460..630
                     /locus_tag="OO439_004333"
     CDS             460..630
                     /locus_tag="OO439_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318520.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="OO439_004334"
     CDS             complement(757..1314)
                     /locus_tag="OO439_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6318521.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="OO439_004335"
     CDS             complement(1311..1586)
                     /locus_tag="OO439_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6318522.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1885
                     /locus_tag="OO439_004336"
                     /pseudo
     CDS             1771..1885
                     /locus_tag="OO439_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          527 a          387 c          458 g          513 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagcctg gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc aggat
//
LOCUS       DAJTVE010000294         1818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000294 DAJTVE010000000
VERSION     DAJTVE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..193)
                     /locus_tag="OO439_004337"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OO439_004337"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..916)
                     /locus_tag="OO439_004338"
     CDS             complement(197..916)
                     /locus_tag="OO439_004338"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6318523.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1057..1332
                     /locus_tag="OO439_004339"
     CDS             1057..1332
                     /locus_tag="OO439_004339"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6318524.1"
                     /translation="MVLESQGEYDSQWAVICSITPKIGCTPETLRVWVRQHERDTRGG
                     DGGLTTAERQRLKELERENRELRCSNDILRQASAYFAKAEFDRLWKK"
     misc_feature    1287..1403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1332..1481
                     /locus_tag="OO439_004340"
     CDS             1332..1481
                     /locus_tag="OO439_004340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318525.1"
                     /translation="MPLLDKLRGQYGVGPVCSELHIAPSTYYHCQQQDIILINAVPVR
                     STMTG"
     gene            1418..1816
                     /locus_tag="OO439_004341"
     CDS             1418..1816
                     /locus_tag="OO439_004341"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6318526.1"
                     /translation="MSATGHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQ
                     LLREGIRVARCTVARLMAVMGLVGVLRGKKVRTTVSRKTVAAGDRVNRQFVAERPDQL
                     WVADFTYVSTWQGFVYVAFIIDVFAGYIVW"
BASE COUNT          457 a          403 c          435 g          523 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacg
//
LOCUS       DAJTVE010000295         1727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000295 DAJTVE010000000
VERSION     DAJTVE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(57..344)
                     /locus_tag="OO439_004342"
     CDS             complement(57..344)
                     /locus_tag="OO439_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318527.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(594..749)
                     /gene="hokD"
                     /locus_tag="OO439_004343"
     CDS             complement(594..749)
                     /gene="hokD"
                     /locus_tag="OO439_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6318528.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(977..1642)
                     /locus_tag="OO439_004344"
     CDS             complement(977..1642)
                     /locus_tag="OO439_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6318529.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          446 a          445 c          383 g          453 t
ORIGIN      
        1 cagttgtata tgtgccatcg gctttctccg gtggcacggt gttacacagc aggagttcaa
       61 tcctgctcaa gattgtagat gagtttattc ttcagcaaaa gcagaaaacc agccttaaaa
      121 ccaatctctt tcaaaacccg taatgatgtg acaaattcgt cctcacgcaa aataaaaccg
      181 tccgtcagaa gtccattaca aaaataaaat aacacagcac cgctcttcct ttgttgagat
      241 tgcaaacatc taatgcggca atggctgaca atcgctccat tctcaacgcg cacagcatag
      301 aggccatttt cactaaaaat ttcacgcaat tcttcgattt tcatcttcag aatccttcca
      361 gataaatagc tctcccctgt tcggggtcca tccctcttct ccctgcgcgc tacttaagtg
      421 agtcgattct atctgcgaag gtgcgcgaat caaattcacc ggaaataaac aacaaaaaac
      481 ccgccgaaac gctgagagat cacagcgatg ggcgggttaa gtgcgggtgc gttgaggatg
      541 cctgacacat cagaggtggg cggggatggg atcagctccc cgcccggtca ctcttacttc
      601 ctggattcgt agtctacgaa gacagcgacc tccgtctggc cggttcggat tcgtacctca
      661 cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg
      721 gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcatgtaa
      781 gaggctaacc tacatgtgca aagcatgaaa ttggcctcag attaatgtta agcgtcttgc
      841 cggacgcgta atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaag
      901 cagatagcct caagcacccg cagtcattct acttacctcg ccaatatgaa atcaatcaga
      961 aaggtgcccc ataaaatcac tccttctctt ctttaccgta gtggagttga ccaattttga
     1021 taagagggcg tccctgagat ttgcggtgta gattggtatc gcgcagagaa tacacacagc
     1081 cacaatattc ctgctgatag aatttttcgc gcttgctgat ttcaatcata cgggacgagc
     1141 cgccctgctt gcgccagtta taatcccagt acaccatgcc cggataatgc gcgacggctc
     1201 gccgcccaca gtcgttaacc tgctgcatat ttttccagcg tgaaatgccc agtgaactgc
     1261 tgatcacact gaaaccattt tcagcggcgt acagcgctgt ccgctcaaaa cgcatgtcaa
     1321 aacacatggt gcaacggatc cctcgttcgg gctcccattc cattcctttg gctcgttcaa
     1381 accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac gccgtgttgt tcagcaaagc
     1441 gaatattctc atccttacga attaaatact ctttctgagg atgaatgttc gggttgtaga
     1501 aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc catcacctca ccggaacatg
     1561 gagcacagca agagtgcagt agtagtttgt ttgccccgtt tgggagctcc aatttgggcc
     1621 gtttgaaatc agcaactgtc ataaatatgt ttattggggt cataaaaata tcaaagagtg
     1681 tagcattaga acggggctat cggaaacaga tgtgtgactg ctccccg
//
LOCUS       DAJTVE010000296         1607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000296 DAJTVE010000000
VERSION     DAJTVE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            97..906
                     /gene="mhpD"
                     /locus_tag="OO439_004345"
     CDS             97..906
                     /gene="mhpD"
                     /locus_tag="OO439_004345"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6318530.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            903..>1607
                     /gene="mhpF"
                     /locus_tag="OO439_004346"
     CDS             903..>1607
                     /gene="mhpF"
                     /locus_tag="OO439_004346"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6318531.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQA"
BASE COUNT          361 a          405 c          479 g          362 t
ORIGIN      
        1 atcttccgcg actgtggtca ctgggcgcag tgggaacatg ccgacgcttt caatcaattg
       61 gtgctgaatt tcctcgcacg cccttaagga atggtcatga cgaagcatac tcttgagcaa
      121 ctggcggcgg atttacgccg cgccgcagag cagggcgaag cgattgcgcc gctgcgcgat
      181 ctgattggta tcgatcacgc tgaagcggcc tacgccattc agcacataaa tgtgcaatat
      241 gacgttgtgc aggggcgtcg cgtggtaggg cgtaaagtgg gcctgacgca tccgaaagtg
      301 caacaacaac tgggcgttga tcaaccggat tttgggacgt tatttgccga catgtgttat
      361 ggcgataacg aaatcattcc tttttcccgt gttctgcaac cccgcattga agcggagatc
      421 gcactggtgt tgaaccgcga tttacccgca actgatatca ccttcgacga actgtataac
      481 gccattgaat gggtacttcc ggcgctggaa gtggtgggga gccgcattcg cgactggtcg
      541 attcagtttg tcgataccgt ggcagataac gcttcctgcg gggtgtatgt catcggcggt
      601 ccggcgcaac gtccggcggg gttagacctg aaaaactgcg ccatgaagat gacgcgtaat
      661 aacgaagagg tttccagcgg gcgcggcagc gaatgcctgg gacatccgct taatgccgcc
      721 gtctggctgg cacgcaaaat ggccagtctg ggtgaaccgc tgcgcgccgg agatatcatt
      781 cttaccgggg cattaggccc gatggtggcg gtgaatgcgg gcgatcgttt tgaagcccat
      841 attgaaggca taggttcagt tgctgcgaca ttttcaagcg cagccccaaa aggaagtctg
      901 tcatgagtaa gcgtaaagtc gccattatcg gttctggcaa cattggcacc gatctgatga
      961 ttaaaatttt gcgtcacggt cagcatctgg agatggcggt gatggtcggc attgatcctc
     1021 agtccgacgg tctggcgcgt gccagacgta tgggcgtcgc caccacccat gaaggggtga
     1081 tcgggctgat gaacatgcct gaatttgctg atatcgacat tgtatttgat gcgaccagcg
     1141 cgggcgcgca tgtgaaaaac gatgccgctt tacgcgaagc gaaaccggat attcgcttaa
     1201 ttgacctgac gcctgctgcc atcggccctt actgcgtgcc ggtggttaac ctcgaggcga
     1261 acgtcgatca actgaacgtc aacatggtca cctgcggcgg ccaggccacc attccaatgg
     1321 tggcggcagt ttcacgcgtg gcgcgtgttc attacgccga aattatcgct tctatcgcca
     1381 gtaaatctgc cggacctggc acgcgtgcca atatcgatga atttacggaa accacttccc
     1441 gagccattga agtggtgggc ggcgcggcaa aagggaaggc gattattgtg cttaacccag
     1501 cagagccacc gttgatgatg cgtgacacgg tgtatgtatt gagcgacgaa gcttcacaag
     1561 atgatatcga agcctcaatc aatgaaatgg ctgaggcggt gcaggct
//
LOCUS       DAJTVE010000297         1538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000297 DAJTVE010000000
VERSION     DAJTVE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            252..1367
                     /locus_tag="OO439_004347"
                     /pseudo
     CDS             252..1367
                     /locus_tag="OO439_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          603 a          275 c          221 g          439 t
ORIGIN      
        1 ctatcagaga acataataac tttttaaaat gagcgcattt tgatagttgt taacgatttc
       61 ctttacgata ctatcactac ccttttttta tacacaaata ttaccgggtg gagataaaag
      121 ggaaatcaaa ggtaattata tcaggaagat ataacgcatt acatttattg tgtgtagaag
      181 aaaatccatt cctgttctaa ttatccaatt taaacatctt agcataaaac aaatgatgaa
      241 taaggaattt tatgtcaatt gactttaccc caggtataat aaatacatat cacggcgata
      301 tttataactg cacaacaaat accgataatg ctaaaacgcc agacacaccg aagtggcctt
      361 gcgataattg ggaagaacaa caacccatta attccacatt ctctggagaa ggatatatct
      421 ctgatcaata tgatttagtg caacaccaac ttcaacaaat taatgcgtgc cacacgaaca
      481 ctacatatac aaatgcagac tactcaaaag ttgtggctca acttgtaagt cttattacta
      541 acattgaaac gataagttcg acacaactta cgcaacagac acaatcaata cttaatcaga
      601 ttaataacat tagatatgaa aaaaacaaaa gtgctgaatg tcgaatcatt gttgtcgcta
      661 atcctaaacc cgacaaggca atcataacta aaattagcgt agaagaggga atacccataa
      721 cattcagtgt ccaaaccatg ttttcagata ctaattttat tgctgaacaa cgagctgact
      781 taccaactaa tataaaagat attcaaagcc tgtatcaaaa aatgacgaag ttatatattg
      841 agcacagtga aaataaaaac aggatgaaag tctttgccgg agccaacttt attgatttca
      901 atatgacagg acagaacctg tccgggttcg ttctaacctt gtcaaggttt tatttcgaag
      961 atctacttaa tataaatttc actgacgcaa atttaggtga cactattttt tacataaaga
     1021 acaccctacc cccaaattat ataaagatgg acaatatctt gacaaacaaa tcgaaggttt
     1081 attttcaaca ttattaacca ttaatgacaa ctttttacga gcaaaagcgg aaattgcttc
     1141 gacaataatt aaatttttag aggccaggat aaccaatctt tcatacaatg acatattaaa
     1201 atatcagcaa gaattccaaa aacaatgtta taagcaagtt aaagccttta cgacactctc
     1261 tcgatacaat aaaattcaaa catgggcaga aatgtctgaa tatcagttcg aagtatttca
     1321 atacgagacg cttaacccca aaaaaatgtc acacacgcct tatttaaaga ggccacttcc
     1381 taatgaaaaa gatataaatt atggggtgga aatagaaata ccatctggca aaagaattcg
     1441 cttatcaaat cattatcaga atataatacc ataatattga ttattagcac tttataatta
     1501 ttgaatgaaa atagatttta tgtaatttta aaacaaag
//
LOCUS       DAJTVE010000298         1470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000298 DAJTVE010000000
VERSION     DAJTVE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO439_004348"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO439_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6318532.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="OO439_004349"
     CDS             complement(1090..1437)
                     /locus_tag="OO439_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCR6318533.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          410 a          323 c          367 g          370 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg atcatcaggg cagtcatttt
     1441 cactgcagcc agcagcagta atgccgatca
//
LOCUS       DAJTVE010000299         1430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000299 DAJTVE010000000
VERSION     DAJTVE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..438
                     /locus_tag="OO439_004350"
     CDS             13..438
                     /locus_tag="OO439_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6318534.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1430
                     /locus_tag="OO439_004351"
     CDS             463..>1430
                     /locus_tag="OO439_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6318535.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DAJTVE010000300         1415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000300 DAJTVE010000000
VERSION     DAJTVE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..566)
                     /locus_tag="OO439_004352"
     CDS             complement(<1..566)
                     /locus_tag="OO439_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6318536.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPA"
     gene            822..1017
                     /locus_tag="OO439_004353"
                     /pseudo
     CDS             822..1017
                     /locus_tag="OO439_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1157..>1415)
                     /locus_tag="OO439_004354"
     CDS             complement(1157..>1415)
                     /locus_tag="OO439_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6318537.1"
                     /translation="RPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYF
                     NKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT          384 a          268 c          349 g          414 t
ORIGIN      
        1 gccgggccgt ggcaaaggga gataacaaaa cggtcatttt tgattgccca ctgcaaagca
       61 gcagctacgt cctggctttc tggtaaacca ataagtgcgc catgaccacc aggaacaaag
      121 attgctgcat attcgctatc agcgttgagg ctggcgacaa catccgcgag tttcttcgga
      181 ttgcggaaca acgatttatg ctgctcaaag aatggcatta ctttttcatc tttgtgcggc
      241 atagcccagt attcaaactt ggtcatcaga ccggaaatgg tcgccacttc gaattcgaaa
      301 ccagcagcat ggagatgata caacggcagc aacgtttcaa tcggatggtt accggtcgag
      361 aacagttttc cgttatcggt tggcaaataa cgttcgtccg cggcgatcac cagaatttta
      421 tgcttaccgc gatacggttt tggatagtcc acgccatcaa gatcagagac gggactggta
      481 tattggctaa gcgaatattc tgaagggaag aatgcattat cttcagcaat atcgacctgc
      541 ggatttttac ttgtttgaac agtcatagtg tattccttat tgttgcttag ttagggtagt
      601 cactatattt gcgactgagc tggtcgctat ttttaaccag tctggaaaat tgagtcaata
      661 tatcgaaagc ggtagtcggc gggtgtaagt atgtaatgat gatgctgcac tggtgttgaa
      721 tattattcac gccaaatttt ccttattgag caacgtcgtc gcaagtggcc tcaatgattt
      781 taggccatta tcaaaggtac ggggcggcgg ggagagattt agtgctgtga aatattgtgg
      841 tactgatgaa tatttatatc gcggattggt agagaaaaga ttatttcatg cactattttt
      901 gcaaatattt ctatatggga agcgtgtttg ttacggatta attcataaag aaatgcattt
      961 gggtgttggt ttgaagagtc gctggttgat gttattgtgc caggaggggc agggtaaggt
     1021 gctgtatcga tatgcattgg caattgcgtg tgccgcagat ggagatgaaa tgccaataaa
     1081 aaagcctgaa ttcgatgagg gacgaactca ggctagtgtt tagttttaca gtaacacgcc
     1141 gcagcacgtt ggctgattag aactggtaga ctaagcccac accaacaacg tcatctgttg
     1201 cgatgccatt gttgtcatag aaatcgtcat ctttatccaa caagtttatt ttatagtcaa
     1261 catatgttga gaagttctta ttgaaatagt aggtcgctcc aatttcaaca tagttgacca
     1321 gatcctgatc aacatactcc tgatgaccat tgttgttata gtcgttaccg ccgaggtctt
     1381 tgccttttga ataaacatat gcaagagatg ggcgc
//
LOCUS       DAJTVE010000301         1384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000301 DAJTVE010000000
VERSION     DAJTVE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1339)
                     /locus_tag="OO439_004355"
     CDS             complement(<1..1339)
                     /locus_tag="OO439_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6318538.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAJTVE010000302         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000302 DAJTVE010000000
VERSION     DAJTVE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            418..873
                     /gene="csgB"
                     /locus_tag="OO439_004356"
     CDS             418..873
                     /gene="csgB"
                     /locus_tag="OO439_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6318539.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            914..1366
                     /gene="csgA"
                     /locus_tag="OO439_004357"
     CDS             914..1366
                     /gene="csgA"
                     /locus_tag="OO439_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6318540.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          408 a          265 c          325 g          368 t
ORIGIN      
        1 taaaaaatat ttcggtgtag tcctttcgtc atgtaaaaag ttgttgtttt ttctccacac
       61 ctccgtggac aaatttttac tccaaaaaga cgaggtttgt cacggcttgt gcgcaagaca
      121 tatcgcagca atcagcgatg ggcaagaaga atgactgtct ggtgattttt gatagcggaa
      181 acggagattt aaaagaaaac aaaatatttt ttgcgtagat aacagcgtat ttacgtggct
      241 tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag ttattaaaaa
      301 tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag gttgcgttaa
      361 caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg tgacaacatg
      421 aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat tgcagccgca
      481 gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt gagtaagtct
      541 tcatttaatc aggcagccat aattggtcaa gctgggacta ataatagtac tcagttacgg
      601 cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa ccgggcaaag
      661 attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag tgccaacgat
      721 gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa aggttctggt
      781 aataaagcaa atattacaca gtatggtact caaaaaacgg caattgtagt gcagagacag
      841 tcgcaaatgg ctattcgcgt gacacaacgt taatttccat tcgactttta aatcaatccg
      901 atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg tattctccgg
      961 tagcgctctg gcaggtgttg ttcctcagta cggcggcggt aaccacggtg gtggcggtaa
     1021 taatagcggc ccaaattctg agctgaacat ttaccagtac ggtggcggta actctgcact
     1081 tgttctgcaa actgatgccc gtaactctga cttgactatt acccagcatg gcggcggtaa
     1141 tggtgcagat gttggtcagg gctcggatga cagctcaatc gatctgaccc aacgtggctt
     1201 cggtaacagc gctactcttg atcagtggaa cggcaaaaat tctgaaatga cggttaaaca
     1261 gtttggtggt ggcaacggtg ctgcagttga ccagactgca tctaactcct ccgtcaacgt
     1321 gactcaggtt ggctttggta acaacgcgac cgctcatcag tactaa
//
LOCUS       DAJTVE010000303         1360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000303 DAJTVE010000000
VERSION     DAJTVE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..652
                     /locus_tag="OO439_004358"
     CDS             <1..652
                     /locus_tag="OO439_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318541.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQ
                     IQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSS
                     QVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLT
                     KDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            728..>1360
                     /locus_tag="OO439_004359"
     CDS             728..>1360
                     /locus_tag="OO439_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6318542.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTF"
BASE COUNT          322 a          334 c          436 g          268 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgccgaaaaa ataggtaaca accaggcgat caccgttacc aataaccaga tccagaacat
      121 tggcgttaat cagatacaga cggttggtgt caacctggtg gaaacggtgg gcagtaacca
      181 gattatcaaa gtgggatcaa accaggttga aaaggtgggg atcattcgtg cgctgacggt
      241 gggtgtggct taccagacga cggtaggcgg cattatgaat acctcggtgg cgttgttgca
      301 gtcctcacag gtagggctgc ataaatcact gatggtggga atgggctaca gcgtcaatgt
      361 agggaataac gtcaccttct cggtgggcaa gacgatgaag gaaaacaccg gacaaacagc
      421 agtttattct gccggtgagc atcttgaact ctgctgtggt aaggcaaggc tggtgctgac
      481 gaaggacgga agcatatttc tcaacggtac gcacattcat ctggaagggg agtcggatgt
      541 gaacggtgat gcgccagtga ttaactggaa ctgtggtgcc acacaacctg taccggatgc
      601 gcctgtgccg aaagatttac cccccggaat gccggatatg cggcaatttt gagtcaggtg
      661 caaaaaatta aaacggaact ggtgcagtga ccagatgaac catattaaaa gcagaaggaa
      721 ttgattcatg ggcggcggaa aaccggcggc gcggcagggc gacatgaccc gcaagggact
      781 ggatattgtg cagggttcag cgggtgtgct gataggtgcg ccgacgggag tggcctgttc
      841 ggtgtgtccg ggagggatta cctatgctaa cccggtgaac ccggtgctgg gtgcgaaggt
      901 gctgccgggc gagacggacc ttgcgctgcc ctgcccgctg ccgtttattc ttttccgcgc
      961 ctacagcagc taccggacca gaacacccgc gccggtgggg gtgtttggtc ccggctggaa
     1021 agcgcccttc gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg
     1081 cggcaggagc attcactttg agccgctgtt tcccggcgag ataagctaca gccgcagcga
     1141 gtcactctgg ctggcgcggg gcggagtggc ggcgcagcac agttcgcagc cgctaagcgc
     1201 gctctggcag gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa
     1261 cagccttcag gggccgtggt ggatactcag ctggccggag ccgcccgcat accgggtact
     1321 gacggtcgtg gtggatggct tcgggcgctc actgactttc
//
LOCUS       DAJTVE010000304         1342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000304 DAJTVE010000000
VERSION     DAJTVE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..485)
                     /gene="umuC"
                     /locus_tag="OO439_004360"
     CDS             complement(<1..485)
                     /gene="umuC"
                     /locus_tag="OO439_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6318543.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
     gene            complement(485..904)
                     /locus_tag="OO439_004361"
     CDS             complement(485..904)
                     /locus_tag="OO439_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6318544.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1277..>1342
                     /locus_tag="OO439_004362"
     CDS             1277..>1342
                     /locus_tag="OO439_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCR6318545.1"
                     /translation="MTEIVADKTVEVVKNAIETADG"
BASE COUNT          402 a          325 c          276 g          339 t
ORIGIN      
        1 ccccccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc cagcgttttg
       61 gtctgggcga tccccatacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt
      121 tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc
      181 tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga
      241 ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga
      301 aacagatctt tttgtttgaa ccagggatcg cccattttaa cacccagcgc ctttgcctca
      361 gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat
      421 aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagagca
      481 aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt
      541 cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc
      601 gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat
      661 ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa
      721 tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt
      781 gattcagatc gatgcgctgt tcgacgtaat ctgctgccgg tgaaggaaag ccacactgaa
      841 caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca
      901 acataataat ctgcctgaag ttatactgtt tttatataca gtaatctgtt cttgccagca
      961 gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc
     1021 tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat
     1081 atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttga
     1141 tatttatcat attaatagaa ataaagacat tgacgcatcc cgcccggcta actatgaatt
     1201 agatgaagta aaatttatta atagttgtaa aacaggagtt tcattacaat ttatatattt
     1261 aaagaggcga atgattatga ctgaaatcgt tgcagataaa acggtagaag tagttaaaaa
     1321 cgcaatcgaa accgcagatg ga
//
LOCUS       DAJTVE010000305         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000305 DAJTVE010000000
VERSION     DAJTVE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1112
                     /locus_tag="OO439_004363"
                     /pseudo
     CDS             1..1112
                     /locus_tag="OO439_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAJTVE010000306         1271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000306 DAJTVE010000000
VERSION     DAJTVE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(724..>1271)
                     /gene="queE"
                     /locus_tag="OO439_004364"
     CDS             complement(724..>1271)
                     /gene="queE"
                     /locus_tag="OO439_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6318546.1"
                     /translation="TWEKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTA
                     RHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGG
                     YDVLSQALERANEIKHPVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCI
                     ETCIARNWRLSMQTHKYLNIA"
BASE COUNT          409 a          270 c          227 g          365 t
ORIGIN      
        1 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
       61 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      121 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      181 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      241 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      301 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      361 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      421 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      481 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      541 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      601 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      661 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      721 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      781 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      841 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      901 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
      961 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1021 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1081 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1141 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1201 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1261 ttcccaggtg t
//
LOCUS       DAJTVE010000307         1254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000307 DAJTVE010000000
VERSION     DAJTVE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(23..>1254)
                     /locus_tag="OO439_004365"
     CDS             complement(23..>1254)
                     /locus_tag="OO439_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318547.1"
                     /translation="IIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAPGWLE
                     LSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRISKTKK
                     PDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFYAVQQ
                     LLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNKESRC
                     SYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTETLIQ
                     LPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEAQIII
                     KRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDMLIFG
                     EPVTRIYPAGDMEEVDA"
BASE COUNT          332 a          297 c          225 g          400 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atga
//
LOCUS       DAJTVE010000308         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000308 DAJTVE010000000
VERSION     DAJTVE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..695)
                     /locus_tag="OO439_004366"
     CDS             complement(<1..695)
                     /locus_tag="OO439_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6318548.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          377 a          199 c          265 g          391 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatattctg
      721 ataaaatgtg atactgggaa ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaaga gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcgtatgcaa gaacgtgcca cggctggctg atggatgttc
     1021 gatagcgcga gtttgaatga aaatcagccg gagatgattt tacataattg ctacggaatt
     1081 attcaataca ggaattgctt gcgtctgcat ggattgacct gaaatattcc cgaaaatttc
     1141 tctaaaaaac tcgaaaaaaa tggtaactgg ttgaatgtat taatatgcaa tggtacgtgt
     1201 cagggattaa aagatgaacg taaatttatt ta
//
LOCUS       DAJTVE010000309         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000309 DAJTVE010000000
VERSION     DAJTVE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            224..1039
                     /locus_tag="OO439_004367"
     CDS             224..1039
                     /locus_tag="OO439_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318549.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          360 a          226 c          232 g          371 t
ORIGIN      
        1 gtggtttaac atattaactt cgctctccac ttaacttttt agttaaggag agcgaaataa
       61 tatcaaagta gcagtaaaac ctataacgta aattcaaatt gttaaattaa ctccctccag
      121 tacacaatac ttctcacgtt agttatgagc gatttctgat agtgcctggt ttaatcagcg
      181 ctttattatc tgcgacgttt atttttattt aagagagatc gcgatgatat cattaaaagc
      241 tccgcacaat aatttgatgc catatacgca acaaagcata cttaatacgg ttaaaaacaa
      301 tcagttacca gaggatatca aaagctccct agtttcctgt gtcgatatat tcaaggtttt
      361 gattaaacaa tattatgatt atccttatga ttgtcgtgat gatttagtcg atgacgacaa
      421 actcattcat ctcatggctg ccgtacgaga ctgtgagtgg tcagacgata acgcactcac
      481 cataaatgtg cagtttaatg atttcccagg attttatgac tggatggatt accctgatca
      541 cccggttaag tttgtttttc gcatacttga aaatcaaaaa gggacagttt gggtatatga
      601 ccaggatgat gcgtttcttg atattaaagc aaatgtgcag gctggacgct ttaccgggct
      661 taagaaatta gtgcagttta tcaattcagt gcgaacagat tgcaaatgta ttcttcttga
      721 acaccatatg cctttgctga gacttttccc taaagggaaa gagtgtatgc atgtggaaaa
      781 atggcttcgc gaaatgtcgt ctattccgga gacagacgcg cccattaagc aggcgcttgc
      841 gcatggatta ttattacact taaaaaatat ctaccctgtt tttcctgaat cactggtgat
      901 gttattgctc tctgttctgg acgtaaaaac atacagagat gatgcacgat taaatgaatg
      961 gatatctaat agagtgcagg aattaggtga cagatattat cctgtaaata agcacgttaa
     1021 aatccgctac actctttagt tcagaaaggg ttccacctgc aagttgttat tccaccagag
     1081 agtgaatcgc aaagcaggta atcatttcat ctgtatatcc gtatgccaaa tggcattcca
     1141 ccgccttttc gccgatattt tgttgtgatc ggcgacactt cgcttaaaa
//
LOCUS       DAJTVE010000310         1188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000310 DAJTVE010000000
VERSION     DAJTVE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..254
                     /locus_tag="OO439_004368"
     CDS             <1..254
                     /locus_tag="OO439_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6318550.1"
                     /translation="LAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVS
                     QDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            272..910
                     /locus_tag="OO439_004369"
     CDS             272..910
                     /locus_tag="OO439_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6318551.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          375 a          232 c          257 g          324 t
ORIGIN      
        1 cgctggcctg ggatgccttc gcatttactg aagtgttact gactcagccg gtgatggtag
       61 tagttggtga aaaagtcggt gccttcggtg cttatcgtga tggactggaa gtctatggcc
      121 gggcgatggt ttcgcaggat cgtcagttag tttcattacc cgatttctca cattatgaac
      181 tgtatgacaa accggaagca gttcaggagg cgctggaaaa agttattcct tttttcaaca
      241 cccatctggg ataagtttca tgagaaatat tatgaaaaat gtactgattc ttggtgctgg
      301 cggtcagata gcccgccatg tgattaatca actggcagat aaacaaacca ttaaacaaac
      361 tttatttgcc cgtcagccag caaaaatcca caaaccctat ccaactaata gcaagattat
      421 tatgggagat gtactgaatc atgcagcact gaagcaggcc atgcagggac aggacgttgt
      481 gtatgccaac cttacggggg aagatttaga tattcaggca aattcagtga tcgctgcgat
      541 gaaagcctgt gatgttaaac gtctgatctt tgttctttcc ctcggtattt atgatgaagt
      601 tccaggaaaa tttggcgaat ggaataatgc agttattggt gagcccctaa aaccattccg
      661 cagggctgcg gatgcaattg aggcttctgg actggaatat acaattcttc gccctgcctg
      721 gctgactgat gaagatatta ttgattatga gcttacctca cgcaatgaac cattcaaagg
      781 tacgatcgta tcacgtaaga gtgtcgcagc attaatcaca gatatcatcg ataaacctga
      841 gaaacacatt ggtgagaata taggtatcaa tcaacccgtg actgatggtg acaagccatt
      901 attcatgtaa accaattaaa tagttaccat cgtaatgtac tatgtgcagt acgatttatt
      961 aaacttatta aggtgaactg atcaatccga tgcgattaac atgtgtagaa ccagtaagaa
     1021 taaaatacgc catgtatata acattacgac ttacatctaa gtcagcataa ttctaatatc
     1081 tgcacatcaa taaaaatgca aacaacttcc ggcaaaaaaa gtgatacaag atcgatgaca
     1141 tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacact
//
LOCUS       DAJTVE010000311         1182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000311 DAJTVE010000000
VERSION     DAJTVE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1182
                     /locus_tag="OO439_004370"
     rRNA            <1..>1182
                     /locus_tag="OO439_004370"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          297 a          265 c          366 g          254 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      721 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
      781 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
      841 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      901 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      961 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1021 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1081 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1141 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ct
//
LOCUS       DAJTVE010000312         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000312 DAJTVE010000000
VERSION     DAJTVE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..154)
                     /locus_tag="OO439_004371"
     CDS             complement(<1..154)
                     /locus_tag="OO439_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR6318552.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWM"
     gene            complement(477..1151)
                     /locus_tag="OO439_004372"
                     /pseudo
     CDS             complement(477..1151)
                     /locus_tag="OO439_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          370 a          172 c          230 g          379 t
ORIGIN      
        1 gcatccagac acagaaggca agaagtagac aactgactga tatccagagg tttcttcgag
       61 caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag tcgcgtaaaa
      121 gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc acacattggt
      181 aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa ttaaggatga
      241 attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg ggcagaatgg
      301 ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct tcaaacgggg
      361 ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa agggttacat
      421 attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt tattcactaa
      481 aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc ctctcttatt
      541 gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa ggtcatgttt
      601 ttaactttta tattcctctg gtttacaact tcctctgtct tgtaagtgtg tatattatat
      661 ggggtatatg ctatttttaa taggatagtg aaacaattcg ttatccacat ctattccgtc
      721 agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa gcttaagtgt
      781 tggggaaaac aaattaaata acatgagctg catagtaggg caattgttct tcccagaaac
      841 atttaaaaaa tttaagtttt tgctctgttt tattatggga aataaagttc caatatcgga
      901 tgtattatta gtatcaatgt gggttatttt attgttgtcc agtcttaaac ttttaataga
      961 tggaagacaa gtcaggaaat caagatttat atttttcaac tggttgttag cagcgtttaa
     1021 tgtctgaaca ttgcagaagt tgctatagaa tattttaatt tcagaaagca ggtttttgtt
     1081 aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta gtttattatg
     1141 ggatagatcc a
//
LOCUS       DAJTVE010000313         1119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000313 DAJTVE010000000
VERSION     DAJTVE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..190
                     /locus_tag="OO439_004373"
     CDS             <1..190
                     /locus_tag="OO439_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6318553.1"
                     /translation="HFYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVLWNWILFRK
                     FSSPEQVENVAAQLLEFA"
     gene            190..480
                     /locus_tag="OO439_004374"
     CDS             190..480
                     /locus_tag="OO439_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6318554.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            477..1031
                     /locus_tag="OO439_004375"
     CDS             477..1031
                     /locus_tag="OO439_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6318555.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          316 a          219 c          317 g          267 t
ORIGIN      
        1 acatttttat ccggacggta gccgcgggcg tcgggcaaaa tccatcgctt ttgcgaatat
       61 ggacgaaacc gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat
      121 tctgttccgt aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga
      181 gtttgcgtaa tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt
      241 cctggctact gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga
      301 acgtgcggaa cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc
      361 cacgatttaa tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg
      421 atgcatgcag aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga
      481 tttacccaac gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg
      541 tctggattca gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga
      601 cggggaatat gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg
      661 aggcgctccg gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc
      721 gggatatgat taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt
      781 tatgtattga tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc
      841 ttcggcaacg gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg
      901 atgcacaccc gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg
      961 tggcagaatt tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta
     1021 ttgctttttg acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag
     1081 gcgaatagca gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAJTVE010000314         1107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000314 DAJTVE010000000
VERSION     DAJTVE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1085
                     /locus_tag="OO439_004376"
     CDS             join(<1..178,178..1085)
                     /locus_tag="OO439_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6318556.1"
                     /translation="LSISEGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEIL
                     QLRKALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYY
                     NWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRK
                     ELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYL
                     AGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYR
                     VIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIF
                     YNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          314 a          264 c          288 g          241 t
ORIGIN      
        1 ctttcgatca gtgaaggcgc ttcccgatta tctcttcctg aaggcacttt aggacaatgg
       61 gttaccgccg ccagaaaagg gctcggtact cctggttccc gcacggtggc tgaactggaa
      121 tctgaaattc tgcaactgcg taaggcgtta aatgaagctc gccttgagcg agatatatta
      181 aaaaaagcaa cagcgtattt tgcacaggag tcgctgaaaa atacgcgtta atcgaacaat
      241 ggcgacaaca atttcccatt gaagcgatgt gtcaggtatt tggtgtatcc aggagcggtt
      301 attacaactg ggtacagcat gaaccctcag acagaaaaca aagtgatgag cggctaaaac
      361 tggagattaa ggtggcacat atccgcactc gcgaaacata tggaacccgg cggctccaga
      421 cggagctggc agagaatggc atcatcgttg gtcgtgaccg actggcacgt cttcgtaagg
      481 agctaaggct acgctgtaag cagaaacgca agttcagagc gactacgaac tcgaaccaca
      541 atctgccagt tgcgccaaat ctgctgaacc agacgttcgc tcctacagca ccaaatcagg
      601 tctgggtggc ggacctgacg tatgttgcca cacaggaggg atggttgtac ctcgctggca
      661 tcaaagatgt ttatacgtgc gaaattgtcg gctacgccat gggagagcgc atgacaaaag
      721 agctgacagg taaagccctg tttatggcgc tcaggagcca gcgcccacct gccgggctaa
      781 tccaccactc tgatcgaggt tcacagtact gcgcatacga ttaccgggtc atacaggagc
      841 agtctggtct gaaaacatca atgtcgcgta aaggtaactg ttacgacaac gctccgatgg
      901 aaagcttctg gggaacgctg aaaaatgaga gcctgagcca ctatcgtttt aataaccggg
      961 atgaagccat ctcagtaata cgggaataca ttgagatttt ctacaatcgt cagcgtcgtc
     1021 actctcgtct ggggaatatc tccccggcag ccttcaggga aaaatatcat cagatggctg
     1081 cttaaaaaaa gaacaaatgg tagtgtc
//
LOCUS       DAJTVE010000315         1099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000315 DAJTVE010000000
VERSION     DAJTVE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            759..1055
                     /locus_tag="OO439_004377"
     CDS             759..1055
                     /locus_tag="OO439_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6318557.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          211 c          233 g          325 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttc
//
LOCUS       DAJTVE010000316         1093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000316 DAJTVE010000000
VERSION     DAJTVE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..453
                     /locus_tag="OO439_004378"
     CDS             28..453
                     /locus_tag="OO439_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6318558.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            500..922
                     /locus_tag="OO439_004379"
     CDS             500..922
                     /locus_tag="OO439_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR6318559.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            919..>1093
                     /locus_tag="OO439_004380"
     CDS             919..>1093
                     /locus_tag="OO439_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318560.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQ"
BASE COUNT          254 a          252 c          338 g          249 t
ORIGIN      
        1 gaatatgtga acaacggagg aaatcccatg cagtatgcga aacctgtcac tctgaatgtt
       61 gaagagtgcg accgtctgtt ttttctgcct tacctgtttg gccaggattt tctgtatgtc
      121 gaagcgtctg tgtgcgcgct ggcgaaaaaa atgatgccgg aatatgaagg cggattctgg
      181 cacttcatcc gcctgccgga cggtggcggt tacatgatgc cggatggcga ccgttttcat
      241 ctggtcaacg gtgaaaactg gtttgaccgt accgtgagtg ctgatgccgc aggcatcatt
      301 ttgacttccc ttgtgattaa ccgccagttg tggctgtacc acgacagcgg ggatgcagga
      361 ctgacccatc tttaccggat gcgcgatgcg cagttgtggc gtcacatcga atttcacccc
      421 gaatgcaacg cgatttacgc agctctggac tgattaacgg acggggcggc aatgccgccc
      481 ctgtaaagga gacgagaaca tgtactgtac tgttaaagaa atcatccgcg atgtactgga
      541 tacagacgtg ccggacagtg aatgcgtttt tgccgtggtg ctgacccgtg gggatgtgcg
      601 ctacatagct caggactgga acctgtcaga cgatgagctg gaaaccgtca tgcagcggct
      661 ggacgatgcc tttgagcatg gtgcggatgt cagcgttgtt cacgacgttg ttcgtgaact
      721 gatggaggaa aagcacgcca gccgtcaggt gacagtcccg gcggtgatgc tggaaaaagt
      781 gatggcgctg gcaggcagtg aaatgaagcg cctgtatgcc gtcgggagtg agaacggggg
      841 cgacggtgat gcattcgtca gggaagaacg cgaagcaatg gacgttgtgt tacaggcgct
      901 ggacggggag cacatgtcat gaatatcagc acagaaaccc gcgaaattct gcgcaattac
      961 aaagccgtga ttaatgcgcg gcgtcgtgaa atggggcaga aaccgctcac cactgcgcag
     1021 attgttgatg aaatctgcga ttttgtggcg aatcagcagg cggttttcct gggcggtcac
     1081 tacatccttc agg
//
LOCUS       DAJTVE010000317         1036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000317 DAJTVE010000000
VERSION     DAJTVE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            284..388
                     /locus_tag="OO439_004381"
                     /pseudo
     CDS             284..388
                     /locus_tag="OO439_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="OO439_004382"
     CDS             728..985
                     /locus_tag="OO439_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR6318561.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          355 a          176 c          197 g          308 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaata
//
LOCUS       DAJTVE010000318         1033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000318 DAJTVE010000000
VERSION     DAJTVE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..616
                     /locus_tag="OO439_004383"
                     /pseudo
     CDS             1..616
                     /locus_tag="OO439_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          382 a          191 c          174 g          286 t
ORIGIN      
        1 aacatgttcc aggttaattt tgttagttca acacaaaacg ccacaatctc ctgggattat
       61 ttactaaaaa taacgcctgt tttaataagc attagcgata tgtattctga agaaaaaatc
      121 aagtttgtcg aaagttgttt aaatgagctt ggagacatta ccgaagaaca attaaaaatt
      181 atgagatttg caattataaa atctatacca agggcaactc ttacagataa attagaaaat
      241 gaattaacaa aagaaatata taaaagctca tcgaaaatca acaattgctt gaacagaatt
      301 aaattaccag agatgaaaga attctcatca gaaaaaaata tatgattaca tcgatataat
      361 cattgaagat tatgaaaata tcaaagaaaa tgcttatctg gtcgatccaa aaattaatta
      421 cactatagat ttaaacatag aagactctag ctcagaggaa ttcctttttg ataatagcct
      481 cgagaaagac gaaaattctt cggacaattg ctttgaggtc gaaaaatata acgcatatga
      541 agcatataac tcagagaatc tatattttac cagagaggaa tatacgtatg attacgacct
      601 tttaaatgca atatagacat aataacttct tacaaaatct ataattaata ccgtagatat
      661 tcattcatct taccctgtgc accaaaagca aaaactcact gacgcgggtt tgaaactgat
      721 ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctacaatat
      781 attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata
      841 aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact
      901 gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc
      961 ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa atcaggtgaa actgaccgat
     1021 aagccgcttt ctt
//
LOCUS       DAJTVE010000319         1008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000319 DAJTVE010000000
VERSION     DAJTVE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..236
                     /locus_tag="OO439_004384"
     CDS             <1..236
                     /locus_tag="OO439_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318562.1"
                     /translation="TPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPALRELMQAK
                     HRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            236..610
                     /locus_tag="OO439_004385"
     CDS             236..610
                     /locus_tag="OO439_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6318563.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            607..>1008
                     /locus_tag="OO439_004386"
     CDS             607..>1008
                     /locus_tag="OO439_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6318564.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          284 a          216 c          284 g          224 t
ORIGIN      
        1 ttacccctga acaattgcgt aaacacgcgc aggaaatgct gcgtcatgct gaacaactcg
       61 aaaaaacggg cataacaaaa gacgctatcc gtaaagatat ggtacccgcg cttcgggaac
      121 tgatgcaggc gaagcatcgc gcacagaaag cagtagatga gctggtggac tgtgtggcag
      181 agctggaaac cagagtcgga aagtttgaaa aactggtgca ggaggtgctg cgctgatgcg
      241 ccatgagttt attttacctt atccgccgac ggtgaatact tactggcgac gtcgtgacaa
      301 cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg gatgtggcgc ttattgtccg
      361 tcagcagcga ctgaaattaa gcctgtccgg aagactggcg ataaagatta ttgcagagcc
      421 accggataag cgccgtcgtg acctggacaa tatcctgaaa gcaccactgg atgcactgac
      481 acatgcggga gtgttaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtggtca
      541 gccagtatct ggtggacgtc tgggggtgaa gatttacccc ataatgcatg aagagcaggt
      601 caaaaaatga aactggaaga tttaccgaaa tactactccc caaaatcccc ttgcctgacc
      661 gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag
      721 ggcatgacac agaatcgggc tgagatgggg ttttctgcgt tcctggggaa aatgggcatc
      781 agtatgaatg acagggcgcg ggcaacagaa ttactggcag attatgcact cagtcggtgc
      841 gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa aaccggtagt gatgcgcatt
      901 atggcttcgt acgcttttga ggattatgcc cgcagcgcag cgagtaaaaa gcagtgccct
      961 tgttgctatg gggaaaaatt tattgaaagc gtagttttta caaacaag
//
LOCUS       DAJTVE010000320          993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000320 DAJTVE010000000
VERSION     DAJTVE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..889)
                     /locus_tag="OO439_004387"
     CDS             complement(<1..889)
                     /locus_tag="OO439_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6318565.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
BASE COUNT          336 a          201 c          231 g          225 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aac
//
LOCUS       DAJTVE010000321          982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000321 DAJTVE010000000
VERSION     DAJTVE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..584)
                     /locus_tag="OO439_004388"
     CDS             complement(<1..584)
                     /locus_tag="OO439_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318566.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAA"
     gene            758..>982
                     /locus_tag="OO439_004389"
     CDS             758..>982
                     /locus_tag="OO439_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318567.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAK"
BASE COUNT          292 a          211 c          220 g          259 t
ORIGIN      
        1 aacgctgcca gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc aatgccaaga
       61 cgaatcaaca atacgggcaa ataagatgcc atccattggt acagcttcag ctcgcgctgc
      121 cttaacattt ttatccaacc gttaccgccc gtggtttttg ccaattgcgg gccatcaaag
      181 cgaaaacccg gcacttcaac ctgcgggtag cggtcggtga tgagcagaaa gccttgctgg
      241 cttttacaca acattttgcg aaacttgtac gcccgccagc aggaaagcag atagataacc
      301 agagcagtaa tattgccagg cggtgttgag ggtttttcgt gcacatgtgc cgctttactt
      361 cgcagataac gcccaaaagg tgcgccaata acagggagct gtgaaatcca ttcgccaatt
      421 agcccggacg attgcccgag ataaatgtgt tctgttggca ttcttgctgc cagttcattt
      481 accaggcttg ccgtgagggg cgatttacct gaaccatcac atcctacaat ggcaataaca
      541 cgcactggcg tggaattaat tattgacata ttacgttgat tcacgaaaaa cccggcagta
      601 aataatgtat tgaatattac gtggtccgtg ctcagaatat ccgttcaacc ttattgtgac
      661 aatggttaat tgtgacattg acctgaatta cgctttcata aaaacatatt aaccaaataa
      721 atatttttaa tggatgttta aattaaagga tatattcatg cagtcaataa cacctccatt
      781 aattgccgtt attggtagcg atggttcagg caagtcaacg gtgtgtgaac atcttattac
      841 cgttgtcgaa aaatatggtg ctgccgaaag agttcattta ggaaaacagg ccggaaatgt
      901 cggtcgtgca gtgacaaaat taccgttgat gggaaaatcc ttacataaaa caattgaacg
      961 aaatcaggtg aaaacagcaa aa
//
LOCUS       DAJTVE010000322          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000322 DAJTVE010000000
VERSION     DAJTVE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..857
                     /gene="htrE"
                     /locus_tag="OO439_004390"
     CDS             <1..857
                     /gene="htrE"
                     /locus_tag="OO439_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCR6318568.1"
                     /translation="IEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARV
                     SYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLH
                     SGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIAL
                     DINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYD
                     EQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIIL
                     NGIRCQIQ"
     gene            874..>977
                     /locus_tag="OO439_004391"
     CDS             874..>977
                     /locus_tag="OO439_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318569.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATV"
BASE COUNT          301 a          185 c          231 g          260 t
ORIGIN      
        1 ctattgaaaa attacttggc actgaacaac ggacttcagg tttccagagt attgatactc
       61 aaatgagcag tgactttaag ggtaataacc aactcaacgt cagtagcagt ggctatagtg
      121 ataacgctcg cgtcagttat agcgtaaata ctggctatac gatgaataaa gccagcaaag
      181 atttgagtta tgttgggggt tatgccagct atgaatcacc gtggggaacc ctggcaggtt
      241 cagtttctgc aaatagcgat aacagtcgtc aagtgtctct cagcaccgac ggtggttttg
      301 tattgcatag cggcggcctg accttcagta atgacagttt tagcgactcc gatacactgg
      361 cggtagttca ggctccaggt gctcaaggag cgcgaataaa ttatggcaac agtactatcg
      421 atcgatgggg ttatggtgtc accagcgctc tttctcctta tcatgaaaac cgtatcgcgc
      481 tggatatcaa cgatcttgag aacgatgttg aattaaaaag taccagtgca gtagctgtac
      541 cgcgtcaggg ttcagtcgtc tttgctgatt ttgaaaccgt gcaagggcaa tcagccatta
      601 tgaacatcac acgaagtgat ggtaaaaata ttccatttgc tgcagatatt tatgatgagc
      661 aaggcaatgt cattggtaat gttggacagg gtggacaagc atttgttcgt ggcattgagc
      721 agcagggaaa tatcagcatt aaatggctcg aagaaagtaa acccgtaagt tgtcttgcgc
      781 attatcaaca aagttcagaa gcagaaaaaa tagcgcaatc tattattctg aatggaattc
      841 ggtgtcagat tcagtaacta caaggaacgt caaatgataa aaataacgcc acataaaata
      901 gcgatactga tgggaatatt attatcacca tcagtatttg caacggatat taatgtagag
      961 tttacagcca ctgtcaa
//
LOCUS       DAJTVE010000323          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000323 DAJTVE010000000
VERSION     DAJTVE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO439_004392"
     CDS             complement(<1..202)
                     /locus_tag="OO439_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6318570.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCN"
     gene            complement(571..>952)
                     /locus_tag="OO439_004393"
     CDS             complement(571..>952)
                     /locus_tag="OO439_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6318571.1"
                     /translation="AREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFD
                     LSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLIS
                     QGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          277 a          221 c          149 g          305 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcatcg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagact gaaaataaat tcacggtgta
      121 agttcttaaa taacaaaaat ggagttctcc agtaatcaga acccccatga tgcttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaattttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagaga tgcttaacag gttttctaaa cggatatcct ttctgggata
      301 tctatctttt atcttaaaag tttttacaga tactttaaag atatctgtat atattctgta
      361 cagtaacttt tcagagtctg aaaattttta taaaaaatga ctagccctct ttgaatctca
      421 tcttccccga atcttcgaaa atatctgtct agttgattcg gtagaatcta cctcatgatg
      481 tatacgtgtt tagtacccgt cacaccagct atgtgacccc cttggcaaaa aagccaaata
      541 tcagctacat ccatgtaact attcagtaac ttatttagca agtctgtaat aagttgctct
      601 agagattcct agctcttcct gaatctgcaa cctcgtttta ccttgtgaaa taagcatctg
      661 tacagcctct gaagaagctt ttacaggtct tcccatatgt accccttcag ctttagcacg
      721 ttcaatacca gcctttctac gctctgctat caagcttctt tcaagctgtg ccaacgttga
      781 cataatgcct aacattgctt cacccatagc gcttgataag tcaaatcctt ctctaaggct
      841 gataactgtc acacctttag cctgtagcgc tttaaccgtt gataacacat caattgtgtc
      901 acgtccaaga cggtctacag ctaccacaac tacggtgtca ccttctcgtg ca
//
LOCUS       DAJTVE010000324          943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000324 DAJTVE010000000
VERSION     DAJTVE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..943
                     /locus_tag="OO439_004394"
                     /pseudo
     CDS             1..943
                     /locus_tag="OO439_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          232 c          285 g          192 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgc
//
LOCUS       DAJTVE010000325          942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000325 DAJTVE010000000
VERSION     DAJTVE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            229..>942
                     /gene="gspA"
                     /locus_tag="OO439_004395"
     CDS             229..>942
                     /gene="gspA"
                     /locus_tag="OO439_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCR6318572.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQ"
BASE COUNT          272 a          190 c          225 g          255 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg acattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc ag
//
LOCUS       DAJTVE010000326          935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000326 DAJTVE010000000
VERSION     DAJTVE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..756)
                     /locus_tag="OO439_004396"
                     /pseudo
     CDS             complement(1..756)
                     /locus_tag="OO439_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          220 a          232 c          242 g          241 t
ORIGIN      
        1 ctccatggta cagacccgtg cgccatcagg aacggtttcg gtgaatctga tgttgccgcg
       61 ttcgtcgaag tgaacaacca gggtgcggcc atcgcctgga attattttgt cagtgggtgg
      121 ggtgttattc tggcgcagtt cggcctccat gcgatcgaac tcagcaatgt aggcttcttt
      181 aaatgcagcg gcttttttgc cagtgaatcc catcaccagg aaaacgaagc cgtttttggt
      241 gatttggtac atcgggcgtt tttcgccttt ggcgtcagtg taggtgacgt cctcaaaatt
      301 gagggcgtta aattcaggtg agcattcagt atttgcgatg gcacgtaaca cattgtcgtg
      361 acgcttgcca aagaactctg cgatcgcaat agacgtagtg acagcgcgac cattttcgat
      421 ggttacgtca gggtgagaaa gggcagggat agtagccatg atggcatcct cgagtgataa
      481 gttaattaac tcaccaccga ggttttccac gaccataagg gtggtgagac gtacaggggt
      541 ggaaataccg gtcactcgag aacccggcca gtcttgcgac tgccctgcac atcccaccat
      601 aatttgaatg gagctgtgca ttacgcataa aaaaaccgct tcagcgcggc tatgcgctca
      661 agtaacgttc gggtttccac gcccggcacc cgttttataa ggtgcaggtg cactataatt
      721 ccacccgttc tggttttcaa tagctacatt caacattttc tctttccttt catcaccgaa
      781 gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac gttaaccagt taacaattaa
      841 cgccggatac agggaaaccc ataaccaccc gatacggggg aactaatcgc tttttaactg
      901 tgccgcgtgc gctgagccgc attcaccgca tcaca
//
LOCUS       DAJTVE010000327          930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000327 DAJTVE010000000
VERSION     DAJTVE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..218)
                     /locus_tag="OO439_004397"
     CDS             complement(<1..218)
                     /locus_tag="OO439_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6318573.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKA"
     gene            complement(218..433)
                     /locus_tag="OO439_004398"
     CDS             complement(218..433)
                     /locus_tag="OO439_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6318574.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          257 a          241 c          186 g          246 t
ORIGIN      
        1 gctttgcatt ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg
       61 tgacatacag tccatacacc aatgatatct ttgtatggta tgtagctgac accttccaga
      121 ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaacagc cccaccacca
      181 atagcagctg caacagcctt gcgtaatgat ggcgacatta ttcacctctc gcagccttac
      241 gcttgtcttc tcttattttg aaatacagat ttgtcagata agtcagaagc cccagaagca
      301 gacttcccag cacaccaatc gcagcccact gtgatggact gacctgatcc aaccactgca
      361 aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt gatatttttt
      421 ccatctgata catatcccgc cccccgacag acctgtgcta tcggaaagaa aaaaggccat
      481 cagcaaaact ctgatggcct gaatcacctt taccaatatt gtatgaaaaa acacgcacga
      541 ctaattgaca ataattttca tttccattaa aaaagatcgc gtagcattct taattcatga
      601 ggaactttac ccgccagcaa tctgagtagc gaaagctgtt catccccaat gtttttgctg
      661 gcgggtcctt ttttctacgg tcctcctccc cggagggggg catttcatta tttttctaat
      721 cgttactggt gagaaggcgg aagaaaaagc cagttcttcg cccctaaata acagcatgat
      781 tccagtccat gcaaaatctg atttccagct cacacatacc gagcactgta caaaaaaccg
      841 ccagccaagg cagttaagag tgtgttcccg gggtttgctt agaatatttt gtaagttgtc
      901 cgaagagata tttacaacac cagaatgatg
//
LOCUS       DAJTVE010000328          918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000328 DAJTVE010000000
VERSION     DAJTVE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..660)
                     /locus_tag="OO439_004399"
     CDS             complement(160..660)
                     /locus_tag="OO439_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR6318575.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT          230 a          281 c          257 g          150 t
ORIGIN      
        1 agaacaacag aaacgacaaa acagaaaaat gcctcacact atgacaggca ggaagggcag
       61 cgccgcaggc gcggccctcc tgtatttcag gcaggcgtaa gcctgccagg gttttccggg
      121 ggcctggcag gccatacccc cgccggagcg gggagtaaat caccgacgga acacatgacc
      181 gtcaataaag gtgaaggagt ccaggaataa atcccgcgca tacgcctcat agtcaaaata
      241 gagcgccacc gtctccggca cgtcagccag caggccggta tcactgacga actcaaccgc
      301 aaaagcctcc tcactgtcag cctcgcccca ccaggcatcg cggaagctgt caaaatccgt
      361 ctcaccggta tcatccaccc agagacgata agcctcttcg cagccttcat cccgcgcctg
      421 gcggaagcct tcaacccagg cccagttgat atggcattca gaggccatat tccccgggaa
      481 tccctcataa tcctgaaaca tcagttccgg gtcggtttca tcctggtgaa gggcacggca
      541 ggcggcgaaa aagtcgcgtt catcatcaaa cgttgtcagg tcaaaccagc ggccagcgat
      601 gcttccacag ttgtatttgt gccaggttcc aacgtataca gcaggtgcaa caacagacat
      661 tcccgaatcc tccatagtca catgttctgc gacaccgggc ggtgccgtgc ctccatcttt
      721 gccgggcctg cagggttgca agggcgcaag ggaagccgca gcctgacgca cacggctggc
      781 gggagttgga gggtgagacc gcgaaatctg agcgggtgaa cgaccggaaa atcacggcag
      841 acgggaagga gggaccgggt gaccgcagcg gttcatctta ccttgcaacc cgcccgcaga
      901 agcccggcta cgatggag
//
LOCUS       DAJTVE010000329          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000329 DAJTVE010000000
VERSION     DAJTVE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..125)
                     /locus_tag="OO439_004400"
     CDS             complement(<1..125)
                     /locus_tag="OO439_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6318576.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
     gene            386..721
                     /gene="iraM"
                     /locus_tag="OO439_004401"
     CDS             386..721
                     /gene="iraM"
                     /locus_tag="OO439_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6318577.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          313 a          209 c          154 g          238 t
ORIGIN      
        1 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
       61 gtcagctttt ccgcaagatc tgacggcggc gccgccctga caacatcata gtgtttaaat
      121 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      181 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      241 aactacaaga cgagatataa accaccctgc atttaaataa acaataaaca acatcagaaa
      301 aataattctg ctctatggtt tacattcaaa aatatcattt atacttttca gaacatcacc
      361 agcaaggcat aaacaaggaa actaaatgaa gtggattgtg attgatacag ttatccagcc
      421 atcatgcgga atatcttttt cagtcatatg gagtaaaata aaattaataa tctggtatca
      481 atcggatgct ttcttacctc ctgaaagtat atttacactg actcacacag gcatcatgct
      541 caataacaaa gtgctacctg taaccattta caacgtagta ccattcaata aaacattctg
      601 gaattcaatc aaaaatagcc aggaatgccc tacaaataca gataacgtat tgaatgaatg
      661 ctttaataac cgttgcactc tgcaaatatg tccttatgga ctaaaacaac aaagtccata
      721 aggagtttac tcacatctga caaaatcaat ataaacagcc cctccggaga ggggctggag
      781 agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca gtaccagcac
      841 ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac agggggattc
      901 accatgccag tttc
//
LOCUS       DAJTVE010000330          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000330 DAJTVE010000000
VERSION     DAJTVE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>908
                     /gene="tuf"
                     /locus_tag="OO439_004402"
     CDS             <1..>908
                     /gene="tuf"
                     /locus_tag="OO439_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6318578.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          210 a          226 c          263 g          209 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccg
//
LOCUS       DAJTVE010000331          890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000331 DAJTVE010000000
VERSION     DAJTVE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /locus_tag="OO439_004403"
     CDS             <1..191
                     /locus_tag="OO439_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6318579.1"
                     /translation="RYYYKGVLWSPGYFASSCGGVPISVIRQYIEQQQTPGQVENRAL
                     YPRPEGWGFTAHRIKSAI"
     gene            complement(209..877)
                     /locus_tag="OO439_004404"
                     /pseudo
     CDS             complement(209..877)
                     /locus_tag="OO439_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          229 a          209 c          200 g          252 t
ORIGIN      
        1 tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag
       61 gtgtgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg
      121 aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac cggataaaaa
      181 gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat
      241 cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt
      301 tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg
      361 catcaatcag ctcaccttca aaatagagtc gagtatccat ggtacggtgg cctgtagcca
      421 tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt
      481 tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg
      541 taaccagtgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa
      601 ttttatccag aactttctgc cctacctagg tagaaggttt tggcaccgga taataagtga
      661 ttgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta
      721 aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac
      781 cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc
      841 ataaagctgc aactttcgca aatgatgtgg tacgcatgaa tagtttcctg
//
LOCUS       DAJTVE010000332          886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000332 DAJTVE010000000
VERSION     DAJTVE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO439_004405"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO439_004405"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6318580.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT          205 a          248 c          216 g          217 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttg
//
LOCUS       DAJTVE010000333          881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000333 DAJTVE010000000
VERSION     DAJTVE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>881
                     /gene="tssI"
                     /locus_tag="OO439_004406"
     CDS             <1..>881
                     /gene="tssI"
                     /locus_tag="OO439_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6318581.1"
                     /translation="GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFC
                     VQYGETDYDFLCRMAAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRT
                     EVSTFCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPG
                     RFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVA
                     SELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGE
                     EIFCDEHGRVRVKFNWD"
BASE COUNT          201 a          241 c          276 g          163 t
ORIGIN      
        1 agggctgcgc cagaacttcc gtatcttcca gaatgaggac atcgaaagca tcctcgcgac
       61 gatcctgaag gaaaacggtg tgaccgagtg gagtccgctg ttcagcgagc cgcatccttc
      121 ccgtgagttt tgtgtccagt acggcgaaac tgattacgat ttcctgtgcc ggatggcggc
      181 ggaggaaggc atcttctttt atgaggagca cgcgcaaaaa agtaccgacc agagcctggt
      241 cctgtgcgac accgtgcgtt atctgccgga gtcctttgag atcccctgga acccgaacac
      301 ccgtaccgag gtaagtacct tctgcatcag ccagttccgc tacagcgcac aaatccgtcc
      361 ttcttccgtg gtgaccaaag actacacctt taaacgaccc ggctgggcag ggcgttttga
      421 tcaggaaggc cagtaccagg attaccagcg cacacagtat gaagtgtatg actaccccgg
      481 acgtttcaag ggtgctcacg ggcagaactt tgcccgctgg cagatggatg gctggcgcaa
      541 caacgcagaa gtggcgcgcg gaacaagccg ttctccggag atatggccgg gacggcgaat
      601 tgtgctgacg gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga
      661 actgcacggc gagcagccgc aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa
      721 taaccacttt gcggtaatac cggcagaccg gacatggcgg ccacagccgt tgctgaaacc
      781 gctggtggat ggcccgcaga gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg
      841 tgatgaacat ggccgcgtgc gggtgaaatt taactgggac c
//
LOCUS       DAJTVE010000334          867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000334 DAJTVE010000000
VERSION     DAJTVE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(66..197)
                     /locus_tag="OO439_004407"
     CDS             complement(66..197)
                     /locus_tag="OO439_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6318582.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(697..773)
                     /locus_tag="OO439_004408"
     tRNA            complement(697..773)
                     /locus_tag="OO439_004408"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Arg,seq:tct)
BASE COUNT          274 a          228 c          154 g          211 t
ORIGIN      
        1 cagacaacag tcaaccgcct gaattgtgaa gtatttaaaa atttctcccg ctaactgata
       61 cccggctaac agtctggcgt tttctttttc agcaacggga aagcaacaac caccacaccc
      121 gccaccagca caccgtcagc cagcactgac attatccggc tgctgcaatg ccattcacaa
      181 aaacagtaag caatcacttt ttaccgtaac aggtgataat ccagatatgt atctacccca
      241 gatgagtaat ccgaagttca tccataccac aggtcctggc tattctgttg tactcctgaa
      301 caagagcaaa taattctgaa ttagcaacca tgaactcatc gcaaaccctc tgtatagcat
      361 cactattcag aataataacg tctcttcccg aaagacgatc aggagtacag aacaaaactg
      421 tcaaacggct gaaggccttt gctcgtgctg cattgactat atcaatacgc tgcctaagga
      481 tgaaacaccc cgacgcctca tcaatattca ctctacccac accatatgaa tgataaatat
      541 ttaatgctga aaaaaccatt agaccgtata acaaacactc aatcaatact taacagaact
      601 tttatttttg acaaacataa aatattttca acaatatcct gagccaggta tatttcagta
      661 taaggctctg ccggaaggaa tctggaagaa tgaatatggc gcgctgtact ggattcgaac
      721 cagtgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgctgaatac
      781 cgataatgga ccgccatcgg ggacccgccc ccgcaccaac aaccctgtta tcgtgtcgtc
      841 tgctcttcct gataagctaa tggcggt
//
LOCUS       DAJTVE010000335          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000335 DAJTVE010000000
VERSION     DAJTVE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            144..849
                     /locus_tag="OO439_004409"
                     /pseudo
     CDS             144..849
                     /locus_tag="OO439_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
     misc_feature    425..541
                     /locus_tag="OO439_004409"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          216 a          202 c          246 g          185 t
ORIGIN      
        1 aggtagccat tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac
       61 gtactgttga atgtgcttgt gttctgaact gaaccgcccc gggaatcctg gagactaaac
      121 ttcctgagaa agaggtaaac aggatgacta aaaatactcg tttttccccc gaagtccgtc
      181 aacgggcagt ccgtatggtt ctggaaagtc agggcgaata tgactcacaa tgggcgacaa
      241 tttgttccat tgctccaaag attggctgta cgccggagac tctgcgtgtc cgggttcgcc
      301 agcatgagcg ggataccggg ggcggtgatg gagggctcac caccgctgaa cgtcagcgtc
      361 tgaaagagct ggagcgtgaa aatcgtgaac tgcgccgcag taacgatatc cttcgccagg
      421 cttccgctta ttttgcgaag gcggagtttg accgcctctg gaaaaaatga tgccactgct
      481 ggataagctg cgtgagcagt acggggtcgg accgctatgc agcgaactgc atattgcccc
      541 gtcaacgtat taccactgtc agcaacagcg acatcatccg gataaacgca gtgcccgtgc
      601 gcagcgcgat gactggctga agaaagagat acagcgcgta tacgatgaaa atcacaaggt
      661 atacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg
      721 cactgtggca cgtctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt
      781 ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc gtcagttcgt
      841 ggcagaacg
//
LOCUS       DAJTVE010000336          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000336 DAJTVE010000000
VERSION     DAJTVE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO439_004410"
     tRNA            54..130
                     /locus_tag="OO439_004410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO439_004411"
     tRNA            173..248
                     /locus_tag="OO439_004411"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="OO439_004412"
     rRNA            432..>842
                     /locus_tag="OO439_004412"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAJTVE010000337          839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000337 DAJTVE010000000
VERSION     DAJTVE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..615
                     /gene="ipaH9.8"
                     /locus_tag="OO439_004413"
     CDS             <1..615
                     /gene="ipaH9.8"
                     /locus_tag="OO439_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6318583.1"
                     /translation="ALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDI
                     ARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVR
                     SREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQKYEMLENEYPQRVADRLKASGLS
                     GDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            complement(616..795)
                     /locus_tag="OO439_004414"
     CDS             complement(616..795)
                     /locus_tag="OO439_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318584.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
BASE COUNT          207 a          233 c          229 g          170 t
ORIGIN      
        1 gcgctcacat ggaacaatct ccggaaaacc ctcctggtcc atcaggcatc tgaaggcctt
       61 ttcgataatg ataccggcgc tctgctctcc ctgggcaggg aaatgttccg cctcgaaatt
      121 ctggaggaca ttgcccggga taaagtcaga actctccatt ttgtggatga gatagaagtc
      181 tacctggcct tccagaccat gctcgcagag aaacttcagc tctccactgc cgtgaaggaa
      241 atgcgtttct atggcgtgtc gggagtgaca gcaaatgacc tccgcactgc cgaagccatg
      301 gtcagaagcc gtgaagagaa tgaatttacg gactggttct ccctctgggg accatggcat
      361 gctgtactga agcgtacgga agctgaccgc tgggcgctgg cagaagagca gaaatatgag
      421 atgctggaga atgagtaccc tcagagggtg gctgaccggc tgaaagcatc aggtctgagc
      481 ggtgatgcgg atgcggagag ggaagccggt gcacaggtga tgcgtgagac tgaacagcag
      541 atttaccgtc agctgactga cgaggtactg gccctgcgat tgtctgaaaa cggctcacaa
      601 ctgcaccatt cataatcaca tcgcataaac cacagaccgg actgactccg gaaaaacaga
      661 ggcccgcccc cgggcctccc cggattcatc cgtttccctg ttcagcctga cagcacgccc
      721 ccgcggccgg atgacagact ccgcttcggt aagcaaagcg gtcttctgtg attccgccag
      781 ttgcggctta ttcattactc aacgtcaaac gcccgaattg aagccaaatc atccagacc
//
LOCUS       DAJTVE010000338          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000338 DAJTVE010000000
VERSION     DAJTVE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..516)
                     /locus_tag="OO439_004415"
     CDS             complement(<1..516)
                     /locus_tag="OO439_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6318585.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DAJTVE010000339          815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000339 DAJTVE010000000
VERSION     DAJTVE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..80)
                     /locus_tag="OO439_004416"
     CDS             complement(<1..80)
                     /locus_tag="OO439_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6318586.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLAS"
     gene            157..564
                     /locus_tag="OO439_004417"
     CDS             157..564
                     /locus_tag="OO439_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6318587.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          262 a          191 c          159 g          203 t
ORIGIN      
        1 atgcttgcca gcctaactcc tgcggcattg gcaagctttg ttttgctgcc aaaaaacgct
       61 atagcatcaa ttttcaacat atcgagctcc ttagattttc ctaaggaaac tagatcgtag
      121 agaaacctaa gtcaagaaaa attagaattc cctaatatga aaaacgaaac cttcggtgct
      181 cgcctcttac ataggcgtaa aaaattaaaa ctgtctcagg ccgcattagg taagctggtc
      241 aaagtggctc acgtaacaat ttctcaatgg gaaagagatg aaacacagcc ggcggggaaa
      301 agattattcg cactgagcca agcgcttcag tgctcaccga cttggcttct ttttggggat
      361 gaagataaac aaccaggcga accgatcccg gataatcagc cagccattct gacagaagat
      421 caaaaagagt tacttcaact gttcgacgca ctgcctgagt cagagcaaaa ggccctgttg
      481 tcagagatgc gtgctcgagt tgaaaatttc aacaaacttt ttgaagaact actcaaagct
      541 cgcaaaagaa gcgcaaacaa ataatcccct tttttctcca caccctgtaa taaaaagcac
      601 aaactttcaa acacttgtgt tttttacatc aaaaaaactt aggtttttct acacaaaaat
      661 cttgaccgcc caccttaggt tattctaaat ttcattcatc aagacaccgc acggtgttct
      721 cagcaaacag ttccgctacc ccggcgttaa ggggaaatgg ggtcaacatg aatactatcg
      781 atcttggcaa caacgaatct ctggtgtacg gcgtg
//
LOCUS       DAJTVE010000340          800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000340 DAJTVE010000000
VERSION     DAJTVE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.345"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            272..>800
                     /locus_tag="OO439_004418"
     CDS             272..>800
                     /locus_tag="OO439_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6318588.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWA"
BASE COUNT          218 a          188 c          181 g          213 t
ORIGIN      
        1 gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatt
       61 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
      121 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      181 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      241 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      301 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      361 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      421 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      481 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      541 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      601 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      661 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      721 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      781 tgaaaaaatc atctgggcac
//
LOCUS       DAJTVE010000341          788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000341 DAJTVE010000000
VERSION     DAJTVE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            211..>788
                     /locus_tag="OO439_004419"
     CDS             211..>788
                     /locus_tag="OO439_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCR6318589.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAJTVE010000342          780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000342 DAJTVE010000000
VERSION     DAJTVE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>780
                     /locus_tag="OO439_004420"
     CDS             <1..>780
                     /locus_tag="OO439_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6318590.1"
                     /translation="NDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQD
                     VAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATM
                     EAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPY
                     DIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTG
                     WVKINITTEDIIYRIVASSVCNRWGDPDIGGLIIAAYQGEAD"
BASE COUNT          200 a          201 c          224 g          155 t
ORIGIN      
        1 aatgactttc tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt
       61 gaggaaatgg tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag
      121 acggcgcagg atgtggcggc agccgcaggt tatgcccgcg cagcagagca ggccaaaaat
      181 gacattgatg ctgcgctgac cggcactctg aaaacggcta accatctgtc tgaaatcgca
      241 gcagcaggcg aaaaggcaca acagaagtcc cgggataatc tggggctgaa aagtgcggcc
      301 acgatggaag cacagagcga catttacgac cggacaaaag gccgtctggc gatacccggc
      361 gcattcggct ttgggtgtgc ttttctgcct gaagatgtta tccgttttga cactaagagt
      421 gatttcctgg cctgggtaag gaatgcgctg ccaggtgaat attccgttgc tggcccctac
      481 gacatcatca tacccgacac acggtttgaa ggggtgctca gcatccggtg gactgatgca
      541 cgccctgaga caacagaacc gaggtacaga gccaaatccc ttacttttta cggcattaac
      601 ggccccattt atcacacccg ctactgctac tggcccatat ccagactgac tggctgggtg
      661 aaaataaata taaccacaga agatattatt tacagaatcg tggcgagctc tgtctgcaac
      721 agatggggag accctgacat tggcgggctg attattgctg cgtaccaggg agaagctgac
//
LOCUS       DAJTVE010000343          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000343 DAJTVE010000000
VERSION     DAJTVE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..396)
                     /locus_tag="OO439_004421"
     CDS             complement(<1..396)
                     /locus_tag="OO439_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6318591.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          229 a          169 c          108 g          227 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttc
//
LOCUS       DAJTVE010000344          731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000344 DAJTVE010000000
VERSION     DAJTVE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..361)
                     /locus_tag="OO439_004422"
     CDS             complement(<1..361)
                     /locus_tag="OO439_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6318592.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKS"
     gene            complement(433..645)
                     /locus_tag="OO439_004423"
     CDS             complement(433..645)
                     /locus_tag="OO439_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318593.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          191 a          170 c          158 g          212 t
ORIGIN      
        1 acgactttag caatgccgta tcccgggttc tgcgtgtcgc aactgtattc agatattgct
       61 ctgcggcttc gctcagagct ggcgtatgtt cccgacctac tgattcgcac aggtaatcaa
      121 cgaaaccaga cagcagttct cgttcgcgag gcgtgatcgc cccaccgacc ggagtccagt
      181 aatcgaaacc cagttgcagg agtttgaaaa aacgcttgtg gaatgcgtag ttacgcacac
      241 gcttaaagtc tgcgtgtatc cactcacctg ttttgatttg atgcagaaaa tcgcaactct
      301 ccggcgtcgc cgggagaagt aatccggaag aggtttgttt gatcagttgt atatgcgcca
      361 tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc cgttatgtag tataccagaa
      421 ttaatgccaa tactaacagg atgctctgac tcgcaattca tccagcagtt tatcatttcc
      481 cataatgtca cttaccctca tcggtaaaaa aattgccttt cgaccattac gatacatcat
      541 tgattttttg gtttcaggga agtaatccat ttcgactata actgacaggt catcacgacg
      601 tatgactgcg tatttactac taaatagttt ctttattttt tccacgatgc ctccaggttt
      661 ataagtacaa acggttatat caacatagag acaaaaggtg atgctgccaa cttactgatt
      721 tagtgtatga t
//
LOCUS       DAJTVE010000345          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000345 DAJTVE010000000
VERSION     DAJTVE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>715
                     /locus_tag="OO439_004424"
     rRNA            <1..>715
                     /locus_tag="OO439_004424"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          203 a          155 c          220 g          137 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accat
//
LOCUS       DAJTVE010000346          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000346 DAJTVE010000000
VERSION     DAJTVE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..>707
                     /locus_tag="OO439_004425"
     CDS             122..>707
                     /locus_tag="OO439_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6318594.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          193 a          152 c          148 g          214 t
ORIGIN      
        1 ttcacaaaaa cacattaatg cagtaactat attttccttt ctggtgataa cgtcgggtta
       61 tcattagctt cttcagctat tttactttta tatcccttgt aaacatcatg gagaatccat
      121 agtgaataaa gtttattctc ttaaatattg ccccgtcacc ggggggctta ttgctgtctc
      181 tgaacttgcc cgcagggtaa taaaaaagac atgccgaaga ttaacgcata ttcttctggc
      241 tggcattcca gcaatctgtc tgtgttactc tcagatatcc ctggcgggta ttgtccgttc
      301 cgatattgcc tatcagattt atcgtgattt cgccgaaaac aaagggcttt ttgtacctgg
      361 tgccaatgat attccggtat atgataagga cggaaaactt gtgggaagac tgggtaaagc
      421 cccaatggcc gatttcagca gtgtgagctc aaatggcgtt gctacgcttg tatcgcctca
      481 gtatatcgtc agcgtaaagc ataacggagg atatcggagt gtgagctttg gtaatgggaa
      541 aaatacatat tcccttgttg accgtaataa ccacccttct attgacttcc atgctccacg
      601 tctgaataaa ctggttacag aagttattcc ctcagcggta acatcagaag gaaccaaagc
      661 caatgcttat aaatacactg aacgttacac cgctttttat cgggtgg
//
LOCUS       DAJTVE010000347          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000347 DAJTVE010000000
VERSION     DAJTVE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>693
                     /gene="ipaH3"
                     /locus_tag="OO439_004426"
     CDS             <1..>693
                     /gene="ipaH3"
                     /locus_tag="OO439_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6318595.1"
                     /translation="TISGTYADYFSAWDKWEKQALPGENRNEAVSLLKECLINQFSEL
                     QLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYLDACDNHLSTLPELPASL
                     KHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPE
                     SLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITYIPENILSLDPTCTIILE
                     DNPLSSRIRESLS"
BASE COUNT          205 a          183 c          121 g          184 t
ORIGIN      
        1 cactatttcc ggtacatatg ctgattactt ttcagcatgg gataaatggg aaaaacaagc
       61 gctccccggt gaaaatcgga atgaagcggt ctccctactt aaagaatgtc tcatcaatca
      121 gttcagtgag cttcaactga atcgtttaaa tctgtcctcg ctacctgaca acttaccacc
      181 tcaaatcact gttctggaaa ttactcagaa tgccctaata tcattaccag aattgccagc
      241 atcgctggaa taccttgacg cctgtgacaa tcacctgtca acacttcctg aattacccgc
      301 atctctgaaa catcttgatg tagataacaa ccaactaacc atgcttcctg aattgcctgc
      361 attgctggaa tatattaatg cagataacaa tcagctaacc atgcttcctg aattacctac
      421 atcgctggaa gtgctctcag taagaaataa ccagctgaca tttctccctg agttacctga
      481 atcactggaa gcgctcgatg taagtactaa tcttctggaa agcctaccag ccgtacctgt
      541 aagaaatcat cactcagagg aaaccgagat atttttccgg tgccgcgaga atcgcatcac
      601 atacattccg gaaaatatac ttagccttga tccgacctgc actatcatcc tcgaagacaa
      661 tcctctgtcc tcacggatca gggagtctct gtc
//
LOCUS       DAJTVE010000348          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000348 DAJTVE010000000
VERSION     DAJTVE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>664
                     /locus_tag="OO439_004427"
     CDS             <1..>664
                     /locus_tag="OO439_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6318596.1"
                     /translation="DTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLIT
                     KEDLAAYKAVERTPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSAD
                     AMQIMAEAEKYAYADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIR
                     PGKLAPYESNQTTHYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAK
                     PGV"
BASE COUNT          186 a          168 c          174 g          136 t
ORIGIN      
        1 cgacacgctg gtgcaggcga acctggcaaa gagcctggag atgattgctg aaaacggccc
       61 ggacgaattc tataaaggca cgattgcgga acagatcgcc caggagatgc agaaaaacgg
      121 tggcttgatc actaaagaag atttagcggc ctataaagcg gtcgaacgca ctccgataag
      181 cggcgattat cgcgggtatc aggtttactc catgccaccg ccatcctccg gcgggatcca
      241 tatcgtacaa atcctcaata ttctggaaaa cttcgatatg aagaaatacg gctttggcag
      301 cgccgatgcg atgcaaatca tggcagaagc ggagaaatac gcctacgccg accgctcgga
      361 atatcttggc gacccggatt ttgtcaaagt cccgtggcag gcactgacca ataaagccta
      421 tgccaaatcc attgccgatc aaattgatat caacaaagcg aagccgtcca gtgagattcg
      481 ccctggcaag cttgcgcctt atgagagtaa tcaaactacc cattactctg tagtggataa
      541 agacggtaac gcggtggcgg tgacctatac gctgaacacc accttcggta cgggcattgt
      601 cgcgggcgag agcggtattc tgcttaataa tcagatggat gatttctccg ccaaaccggg
      661 cgta
//
LOCUS       DAJTVE010000349          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000349 DAJTVE010000000
VERSION     DAJTVE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..643
                     /locus_tag="OO439_004428"
     CDS             <1..643
                     /locus_tag="OO439_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6318597.1"
                     /translation="GKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKK
                     FPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFR
                     SIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIR
                     RGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          218 a          108 c          164 g          153 t
ORIGIN      
        1 aggaaaaatc atcacaactg atgcgatggg ttgccagaaa gatattgcag agaagataca
       61 aaaacaggga ggtgattatt tatttgcggt aaaaggaaac caggggcggc taaataaagc
      121 ctttgagaaa aaatttccgc tgacagaatt aaataatcca gcgcatgaca gttccgcaat
      181 gagtgaaaag agtcacggca gagaagaaat ccgtcttcat attgtttgcg atgtccctga
      241 tgaacttatt gatttcacgt ttgaatggaa agggctgaaa aaattatgcg tggcagtctc
      301 ctttcgctca ataatagcag agcaaaagaa agagcccgaa atgatggtca gatattatat
      361 cagttctgct gatttaaccg ctgagaagtt tgcgacagcg attcgaaatc actggcacgt
      421 ggagaataat ctgcactggc gtctggacgt ggtaatgaat gaagacgact gcaaaataag
      481 aagaggaaat gcagcagaat tattttcagg gatacggcac attgctatta atgttttgac
      541 gaatgataag gtattcaagg cagggttaag acgtaagatg cgaaaagcag ccatggacag
      601 aaactatctc gcgtcagtcc ttgcggggag cggactttcg taa
//
LOCUS       DAJTVE010000350          625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000350 DAJTVE010000000
VERSION     DAJTVE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.355"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..292)
                     /locus_tag="OO439_004429"
                     /pseudo
     CDS             complement(1..292)
                     /locus_tag="OO439_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(428..535)
                     /gene="rprA"
                     /locus_tag="OO439_004430"
     ncRNA           complement(428..535)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO439_004430"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          187 a          117 c          148 g          173 t
ORIGIN      
        1 caataaaatt gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca
       61 tggtgttgtt gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc
      121 gttgctgacg catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat
      181 tttcccactg acgccagctt tcactgtcac cggtctgggc aatctaggtc gcgcattcat
      241 caatagtcat ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat
      301 ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag
      361 agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg
      421 aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt
      481 tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct
      541 aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt
      601 aaagaatttg cgacaggtac tacca
//
LOCUS       DAJTVE010000351          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000351 DAJTVE010000000
VERSION     DAJTVE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.356"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..87)
                     /locus_tag="OO439_004431"
     CDS             complement(<1..87)
                     /locus_tag="OO439_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6318598.1"
                     /translation="MASVSISYPSCSATDGVVRNCKSTAGHQR"
     gene            143..622
                     /locus_tag="OO439_004432"
                     /pseudo
     CDS             143..622
                     /locus_tag="OO439_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          149 a          139 c          143 g          191 t
ORIGIN      
        1 gcgctgatgt ccggcagtgc ttttgcagtt acgcaccacc ccgtcagtag ctgaacagga
       61 gggatagctg atagaaacag aagccactgg agcgcctcaa aaacaccatc atacactaaa
      121 tcagtaagtt ggcagcatca ccgccgttgt ctacacattt attctctcgg tgctgattta
      181 ccgggaagta aaatggcgcc atttacccaa actgattctg gagtcggttg ttaccacttc
      241 gattgtatta ctgttgatcg gtttttccgt gggcatgtcc tgggcaatga ctaatgctga
      301 tattccttat atgatcagcg acgcattgat ggggatttct gataacccac tgatcatatt
      361 gctgctgatt aatatcgtct tattaattgt cggcatcttt atggacatga caccggctgt
      421 actaatcttt acaccgatct tcttgccgat tgcacaagag ctgggaatgg acccagtaca
      481 tttcggtatt atgatggtgg ccaacctttg tattgggctg ttaacaccgc cggtgggtag
      541 tgcgctattt gttggttgtt ctatctcggg tgtgaagatt caacatttaa tcaaaccatt
      601 gctgccattt tatgctagca tt
//
LOCUS       DAJTVE010000352          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000352 DAJTVE010000000
VERSION     DAJTVE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>615
                     /locus_tag="OO439_004433"
     CDS             <1..>615
                     /locus_tag="OO439_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6318599.1"
                     /translation="FLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILD
                     SLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAV
                     VSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLS
                     AVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTE"
BASE COUNT          152 a          140 c          140 g          183 t
ORIGIN      
        1 tttttatttg tttcaccatt acttaatccc attatcgtcg gactaatgtg gatgaccttt
       61 ggttggaaag tcaccttgtt gtacgcgatt atcgcagccg gcgtctccgt tcttgccagt
      121 attatcctgg attctctggg gtttgaacgt catatcattg ccagtaaaag ctcatcagca
      181 aattgttgtg ctccagcaaa aacttcgccg ggaacgacat atacgcccat aaaagtgagt
      241 tgttgtagtc cagcggctaa agccattgag aaccccgtag ttacctgttg cagtaccaaa
      301 gctgtagtaa gtattaatcc cataaaacta gccaccaaag atgcgttgca acagtttaaa
      361 gatgtactgc catatctttt gttaggagta ttaataggat cttttattta tggctttatt
      421 ccctcagcgt ggattgccgc tcatgcagga gcagataatc ccttcgccat cccattaagc
      481 gccgttgtcg gtattccgct gtatatccgg gctgaggcgg ttatccctct ggcatctgtt
      541 ctgatgacaa aaggaatggg tctgggagca ttaatggcat taatcatcgg cagtgccggc
      601 gcaagcctga cggaa
//
LOCUS       DAJTVE010000353          612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000353 DAJTVE010000000
VERSION     DAJTVE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>612
                     /locus_tag="OO439_004434"
     CDS             <1..>612
                     /locus_tag="OO439_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6318600.1"
                     /translation="QGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKW
                     YISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQ
                     LSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMS
                     KSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWR"
BASE COUNT          176 a          150 c          164 g          122 t
ORIGIN      
        1 cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa ctgggctgga
       61 tgcgctaccg gaacagccgt caggtcacgg gtgttgtgaa aaatgtcact gtcagccagt
      121 cctgcggtaa gtggtacatc agtattcaga cagaaagtga agtatcaact ccggttcacc
      181 cttcagcatc aatggtcggg ctggatgctg gcgtggctaa actcgccacg ctgtcagatg
      241 gcacagtctt tgagcctgta aacagttttc agaaaaacca gaagaagctg gcgagacttc
      301 agcgacagtt aagccgcaag gtcaaattca gcaacaactg gcagaagcag aaacgcaaaa
      361 tacagcgact gcattcctgt atcgcaaata tccgcaggga ctaccttcac aaagtcacaa
      421 cgaccgtcag caaaaaccac gcaatgatag tcattgagga tttgaaggtc agcaacatgt
      481 caaagtcagc agcgggtacg gtcagccagc cggggcgcaa tgtccgggca aaatcaggtt
      541 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagtacaagc
      601 agctctggcg tg
//
LOCUS       DAJTVE010000354          610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000354 DAJTVE010000000
VERSION     DAJTVE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>610)
                     /gene="rlhA"
                     /locus_tag="OO439_004435"
     CDS             complement(<1..>610)
                     /gene="rlhA"
                     /locus_tag="OO439_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6318601.1"
                     /translation="EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRR
                     EAADMLDAARLASYQRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLID
                     AAYEAHEEKGEVPVMITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFD
                     CRPCEMHVIGKIKNHILKMPLPGSVVASVSPDELLKTLPKRKG"
BASE COUNT          135 a          164 c          144 g          167 t
ORIGIN      
        1 acccttttcg cttaggcaat gttttcagca gctcatccgg acttacggat gccactacgc
       61 ttcccggtaa cggcattttc agtatgtgat ttttgatttt gccaatgacg tgcatctcgc
      121 atgggcggca atcaaacttt agcgttaata cttcatcgcc gttaaccagt tgcattggcg
      181 tcgccttcca gcttttgata ttgcctttcg cctgtttcgg gcacagatta aaggcaaagc
      241 gcagacaatg cttggtgatc atcaccggga cttcgccctt ctcttcatgt gcctcatacg
      301 ccgcgtcaat cagctgcaca ccatagcgat gataaaattc acgcgctttc tggttgtata
      361 cgttcgcgag gaaactcaga tgcgtttgcg gaaaaaccgg cgcaggatca gcaaccggtt
      421 tacggctgcc gcgctggtaa ctggcaagac gcgcagcatc cagcatgtca gcagcttcac
      481 ggcggaactg gtttaacaga ctgtttggta caaacagcgc ccccggcaaa ttaatttgca
      541 catcgcgggc gtaatagagg gtttgcccca gttttgccag accatccttc agattgttca
      601 ttgctttttc
//
LOCUS       DAJTVE010000355          604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000355 DAJTVE010000000
VERSION     DAJTVE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            68..313
                     /locus_tag="OO439_004436"
     CDS             68..313
                     /locus_tag="OO439_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318602.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(330..>604)
                     /locus_tag="OO439_004437"
     CDS             complement(330..>604)
                     /locus_tag="OO439_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6318603.1"
                     /translation="TDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLASVIWR
                     VFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          186 a          142 c          147 g          129 t
ORIGIN      
        1 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
       61 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      121 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      181 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      241 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      301 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      361 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      421 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      481 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      541 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      601 gtaa
//
LOCUS       DAJTVE010000356          603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000356 DAJTVE010000000
VERSION     DAJTVE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..160
                     /locus_tag="OO439_004438"
     CDS             <1..160
                     /locus_tag="OO439_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025245096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6318604.1"
                     /translation="QFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPT
                     VESSEKTS"
     gene            249..603
                     /locus_tag="OO439_004439"
                     /pseudo
     CDS             249..603
                     /locus_tag="OO439_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          154 a          161 c          182 g          106 t
ORIGIN      
        1 ccagttcccg gtagataaat ggcatggtct gatggagaac ccaacaacag cagatgccat
       61 cctggacaga ctggtacata actcacacag agtggtcctt cagggagagt ccctgaggaa
      121 aaatcccccg acagtggaaa gcagcgaaaa aacgagttaa aaagcggtag aaaaagttaa
      181 gttcccggtt gatcgaatgg atgcgaatcg ctgatcgaac ttcgatgcca atccgtgatc
      241 gaatagagcg gaatacgcag ctggtactga ccacgcaggc gcagcgggca gaagaagccc
      301 ggcagcaggc catttccggc gggacggaac cgtccgcttt tcctgatacc ctgccgggtt
      361 acaccgaata tggccgggac aacggcatct gtctgccgtg tggctgacgc acgacccgga
      421 atacccggag aatttacctg ccgcgccgct ggtgcgctat ggctggacgc cacgcggcga
      481 actggcggtg gtgtatgacc gtagtggcaa acaggtgcgc agctttactt acgatgataa
      541 ataccggggc cggatggtgg cgcaccgtca cacgggccgg ccggaaatcc gttaccgtta
      601 cga
//
LOCUS       DAJTVE010000357          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000357 DAJTVE010000000
VERSION     DAJTVE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..>591
                     /locus_tag="OO439_004440"
     CDS             116..>591
                     /locus_tag="OO439_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6318605.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVG"
BASE COUNT          158 a          131 c          152 g          150 t
ORIGIN      
        1 ctactactca ctcacgacgc acaatctgaa aaccgttatg gaacagctgg ctcagggtaa
       61 acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa cggcaatgaa
      121 aattagcgat ggaaactggt tgattcaacc tggcctcaat ttgattcacc cgcttcaggt
      181 gttcgaggtt gaacagcagg ataatgaaat ggtggtctat gctgcccccc gtgatgtgcg
      241 tgaacgtacc tggcagcttg atacgccttt atttacgctg cgctttttct ccccacagga
      301 aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg ctgaataacg gtcctcatta
      361 tccgctcaat attttgcagg acgtgaaggt cacaatcgaa aacacagaac gttatgctga
      421 gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt cactggattt
      481 tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataatggct acgtgcagga
      541 cacgaataat caacgcaatt atatgtttga gcggcttgat cttggcgttg g
//
LOCUS       DAJTVE010000358          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000358 DAJTVE010000000
VERSION     DAJTVE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..72
                     /locus_tag="OO439_004441"
     CDS             <1..72
                     /locus_tag="OO439_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbriae assembly protein"
                     /protein_id="HCR6318606.1"
                     /translation="RALASGVQPGVEKADAIFMINYN"
     gene            complement(75..>587)
                     /gene="fimZ"
                     /locus_tag="OO439_004442"
     CDS             complement(75..>587)
                     /gene="fimZ"
                     /locus_tag="OO439_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6318607.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          180 a          103 c          116 g          188 t
ORIGIN      
        1 cgagcgttag ccagcggtgt tcagccaggt gtggagaaag cggatgcgat atttatgatc
       61 aattataatt aatattatat taattcgtat aatttggcgt agtcgataag ctctacaatt
      121 gaatgcaaac ctagtttgcc ataaatatta gatttatgcg cactaactgt tttattgcta
      181 agtaataact tatcggcaat ttctttatta gataatccgc taaccagata acgtaatatg
      241 gtcacttcac gattagatag cacagtgacc gttgaactat tcgtactaca tttattgctt
      301 tttatatagt tgagcgtttc gctaggaaaa aacgtgtatc cggagaggat catctgaacg
      361 ggatgaaaaa tatcattctg atcattgcat ttactgacaa aaccgttagc accagcttgt
      421 atcgctctgc cagcataaaa gcattctgat ttcgatgata aaaataacac tttcactgtg
      481 ctctggattt gtttgatcct tttcaggaag gtaaaaccgt ctgttccggg caagtctata
      541 tccataatga ttaaatcaac aggacgggtt cggagataat cgatggt
//
LOCUS       DAJTVE010000359          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000359 DAJTVE010000000
VERSION     DAJTVE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..578
                     /locus_tag="OO439_004443"
                     /pseudo
     CDS             1..578
                     /locus_tag="OO439_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          137 a          147 c          162 g          132 t
ORIGIN      
        1 tcagcgctat ctctgctctc attgccgtaa aacatggcaa ctacagttca cttacaccgc
       61 ctctcagccc ggtaagcacc agaaaatcat tgatatggcc atgaatggcg tcggatgtcg
      121 cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact taaaaaactc
      181 aggccgcagt cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt ctgcgctgaa
      241 atggacgaac agtggggcta cgtcggtgct aaatcacgtc agcgctggct gttttacgcg
      301 tatgacagga tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac tctggccaca
      361 ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatagat gacggatggc
      421 tggccgctgt atgaatcacg cctgaaggga aagctgcacg ttatcagcaa gcgttacact
      481 cagcgcattg agcgacataa tctgaatctg agacaacatc tggcaaggct gggacggaag
      541 tcactgtcgt tctcaaaatc ggtggagctg catgacaa
//
LOCUS       DAJTVE010000360          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000360 DAJTVE010000000
VERSION     DAJTVE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..302)
                     /locus_tag="OO439_004444"
     CDS             complement(<1..302)
                     /locus_tag="OO439_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6318608.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDG"
BASE COUNT          198 a          111 c           99 g          169 t
ORIGIN      
        1 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
       61 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
      121 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
      181 acttcacaag tagtgtcgac aatttcaccg acgaagtgaa caacaccatc ttctgccagt
      241 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
      301 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
      361 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
      421 attacgcaaa aataaaatat aaattcattg tttaaatatt atttaaacag taaaaaatca
      481 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagataaat
      541 ttattaatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTVE010000361          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000361 DAJTVE010000000
VERSION     DAJTVE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>573
                     /gene="adrA"
                     /locus_tag="OO439_004445"
                     /gene_synonym="dgcC"
     CDS             <1..>573
                     /gene="adrA"
                     /locus_tag="OO439_004445"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6318609.1"
                     /translation="ASRAVDPLSREIYNLKTDAVLAGMWVGVMGVNVLPSTAMLMIMC
                     LNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWWLSLPIIVVYPLLFG
                     WVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFDNCRRHNRDATLLII
                     DIDHFKSINDTWGHDVGDEAIVALTRQLQI"
BASE COUNT          126 a          131 c          164 g          152 t
ORIGIN      
        1 tagcgagcag ggccgtcgat ccgcttagcc gggaaattta caacttaaaa accgatgcag
       61 tattagcggg aatgtgggta ggcgtaatgg gcgtaaacgt gctgccttcc accgctatgt
      121 tgatgattat gtgtctgaat ttgatggggg caggcggccc ccgtctgttt gtcgcgggtc
      181 tggtgttgat ggtggtttcc tgccttgtca ccctcgagct tacgggcatt accgtgtcgt
      241 tcaatagtgc gccgctggaa tggtggctct cccttcccat tattgtcgtt tatcctctgc
      301 tgtttggctg ggtcagctac cagacggcaa ccaaactggc ggaacataaa cgcaggttgc
      361 aggtcatgag tactcgcgac ggcatgacgg gcgtgtataa ccgacgtcat tgggaaacta
      421 tgttacgcaa tgaatttgat aactgtcggc ggcataatcg cgatgcaacg ttactgatta
      481 tcgatatcga ccatttcaag agcatcaacg atacctgggg tcatgatgtg ggcgatgaag
      541 cgattgtggc gcttacccga cagttacaaa tta
//
LOCUS       DAJTVE010000362          560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000362 DAJTVE010000000
VERSION     DAJTVE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.367"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(251..>560)
                     /gene="tabA"
                     /locus_tag="OO439_004446"
     CDS             complement(251..>560)
                     /gene="tabA"
                     /locus_tag="OO439_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6318610.1"
                     /translation="SEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGTPDT
                     DWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA
                     "
BASE COUNT          155 a          140 c          107 g          158 t
ORIGIN      
        1 tgcccctcgg cattacgggc aaaattattc attccaacac gccaggtatt taaaacttca
       61 cttttttgtt taaaattgtc ggctaatgta atgtgttgag aataattcaa atctgatatt
      121 ttgctcatga cgaactcctt gagttaataa gaacgtctaa gcattacaaa ttttactcac
      181 aaaaaaatat caaaaaacgc tcttttcaca aactcgccgt aaacctggag ttatttacgg
      241 cgaaaagtca tcacgccatc agcatcttca ctactgcttt gcgaacctgg gctggtgcac
      301 ccactgcgca cagcggttta tgcacttccc ccggataaaa cacaacaaaa tcaccttcat
      361 taaggataac tgttttctca tcaacgcctt ccggcaaaaa tgcgatgtct ttatcagcta
      421 accagtcggt atccggcgtg cctgcaggtt gcgtgctgaa ggtcatgcct tcctgacctt
      481 ttaacacaat ctgaatgtcg agatagcggg catggtactc cgtacggcga gcttcgtgcg
      541 gctcggtcat atcttccgag
//
LOCUS       DAJTVE010000363          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000363 DAJTVE010000000
VERSION     DAJTVE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..533)
                     /locus_tag="OO439_004447"
     CDS             complement(<1..533)
                     /locus_tag="OO439_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6318611.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPA"
BASE COUNT          126 a          155 c          140 g          138 t
ORIGIN      
        1 gctggcgcgt tgataaagat cgagaaatca caaaattgcg caagctggca ccacttctcg
       61 tcgtccagca acaaccagtt tccttcgacg atgacgagtg gtgcggtaac gtgcaacgca
      121 tcttcaacag gatcatgctt ttgtcgatcg tactgcggcc acgtacaatc cccttccacc
      181 acctggcgca gattttccgc cagtttcgca acgtcaaatg tctctggtgc gcctttgaag
      241 gggcgcaatt gatgcgcgtc cagccagcta ttgtaatggt gaaaaccatc catcgggagc
      301 gtctgaatag cgggcagctc cgggtcttgt tgcacgagat attcccagaa ggtggttagg
      361 gtagatttac ccgtccccgg cggagcgcaa agaaaaacca ccgtacgccg ctgcggattt
      421 accgtctgta aggccgccag catacgcagc aacggtttat gcacgcgttc tatctcttca
      481 tcatggtact gcgcttgcac gttcagccca ttcaccgtta attcaatttt cacggcgtta
      541 attcctttaa ggtggtcgt
//
LOCUS       DAJTVE010000364          558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000364 DAJTVE010000000
VERSION     DAJTVE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..386)
                     /locus_tag="OO439_004448"
     CDS             complement(<1..386)
                     /locus_tag="OO439_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6318612.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEA"
BASE COUNT          158 a          133 c          124 g          143 t
ORIGIN      
        1 gcttccgcca gcagaaacca gcaaatctgc gcgacgtggg tgccggtggt gatgtgaatt
       61 aaatagtctt ctttttctgg ctgaaactcg taaccacggg cgaaatcatg caggcaggcg
      121 taaacctctt cgaaatccca cgggttatgc agttcaatct caacgcccac gacttctgtt
      181 tctggcgaaa cgctggcgat atcgcgttta agcgtttcga atagcgagcg cgagcgagtg
      241 tcgtgcaaca attccagccg atcgatgact aacgattctt gctggcataa acaaagcgtc
      301 ggacgccatt tcgaccagcg ctgactgccg cgtccggcat aatccagcac ggtaccgaca
      361 aagccaaaag ccactgtttt acgcatctta gatatcctta taaaagacga tatttcatta
      421 tcggtgaatt ggttttgtgg aaaatctccg tttacggacg taaagattta acctatttaa
      481 aaacaatgaa ttaaaaaatt aatctgctta tttaattttc tggcacgacg gttgcaatta
      541 tcacgacagc aaacaacg
//
LOCUS       DAJTVE010000365          553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000365 DAJTVE010000000
VERSION     DAJTVE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>553
                     /locus_tag="OO439_004449"
     CDS             <1..>553
                     /locus_tag="OO439_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6318613.1"
                     /translation="WSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEG
                     LVTHWHYDEADRLTHRTVNGETAE"
BASE COUNT          135 a          165 c          163 g           90 t
ORIGIN      
        1 tggagccgtt acggtcagtt gctgagcttc accgactgtt ccggttatgt aacccgttat
       61 gaccatgacc gcttcgggca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      121 cgcgcatacg acagccgtgg acagttaatt gccgtgaaag acacgcaggg ccatgaaacg
      181 cggtatgaat acaacgccgc cggtgacctg accaccgtca ttgccccgga cggcagcaga
      241 aacgggacac agtacgatgc gtggggaaaa gctatctgta ccacgcaggg cggtctgacg
      301 cgcagtatgg aatacgatgc tgccggacgg gtcatccgcc tgaccagtga aaacggcagc
      361 cacaccacct tccgttacga tgtactcgac ctgctgatac aggaaaccgg ctttgacggc
      421 cgcacacagc gttatcacca cgacctgacc ggcaaactta tccgcagcga ggatgagggg
      481 ctggtcaccc actggcacta tgacgaagca gaccgcctca cgcaccgcac cgtgaatggc
      541 gaaaccgcag agc
//
LOCUS       DAJTVE010000366          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000366 DAJTVE010000000
VERSION     DAJTVE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.371"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>548
                     /locus_tag="OO439_004450"
     CDS             <1..>548
                     /locus_tag="OO439_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6318614.1"
                     /translation="IRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDHVRM
                     STGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAVKLV
                     MQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSKVNF
                     DTQQMIHFINRFNMNKGLIDKN"
BASE COUNT          203 a           73 c           90 g          182 t
ORIGIN      
        1 attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac cagagcagag
       61 tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt tatgtttgat
      121 catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt ggaattaact
      181 attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa taacattata
      241 aaaatgattg atacattgcc agataatgca atgatattga aatccgttct ggcagtaaaa
      301 ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga gaatatgaaa
      361 aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta tatacattct
      421 ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa ggtgaatttc
      481 gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg gctgattgat
      541 aaaaataa
//
LOCUS       DAJTVE010000367          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000367 DAJTVE010000000
VERSION     DAJTVE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          152 a          113 c          131 g          148 t
ORIGIN      
        1 tctgcccttt aataatctac ggacagatta ctttctgtac ttccagagcc tgtttctgtg
       61 agtcaaacat gagctggttt ttaccgacta cacccaagta aatatattcg atatctccca
      121 tttgccgtat tttcgtaaaa ttcaacagga aatattcaac gggagtgaga gaactgctgt
      181 ccggggagga ggtacagacg caaaaaaacc gactggtgag gccggttttt ttgcgattcc
      241 ggatcgaagt tggtagccct gaccggaaga taactgccta taactgacta tgaaataata
      301 cggctgaaat aatatttctg caagcacagt ataagtatat actcggtgtg tcagctttga
      361 ggcacgttct ttccggcata tcgttcaccg ggatgaacag gttgcaagtc ctggccaaaa
      421 catcaggtgg tgctggtttt ggtgctctgg ccggagaaga actgtctata actgacacta
      481 tagaaaacgt ggcgacaggc aatgtaaggg caaaccacgg tgttctttct ccattgatgg
      541 agaa
//
LOCUS       DAJTVE010000368          536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000368 DAJTVE010000000
VERSION     DAJTVE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.373"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..153)
                     /locus_tag="OO439_004451"
     tRNA            complement(77..153)
                     /locus_tag="OO439_004451"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Arg,seq:tct)
     gene            complement(167..243)
                     /locus_tag="OO439_004452"
     tRNA            complement(167..243)
                     /locus_tag="OO439_004452"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Arg,seq:tcg)
     gene            complement(251..326)
                     /locus_tag="OO439_004453"
     tRNA            complement(251..326)
                     /locus_tag="OO439_004453"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Ile,seq:cat)
     gene            complement(429..>536)
                     /locus_tag="OO439_004454"
     CDS             complement(429..>536)
                     /locus_tag="OO439_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6318615.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          150 a          136 c          130 g          120 t
ORIGIN      
        1 cagacaactg tcaaccgtct gaattgtgag acatttaaaa aaaaggcccg caaaagcgag
       61 ccagggaaaa taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
      121 ggcaattgct ctgtccggct gagctaacaa cgcatgatgc agataatgga ccgccatcgg
      181 ggacttgaac cccgcgcagc cagcttcgaa ggctggcgct ctttcctgat gagctaatgg
      241 cggtatgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
      301 cgctctcacc actgagctaa agggccggga gcagaataat aacggtccgt aattaattcc
      361 gcaataaaaa acccgctcgg cggcgggttg tagaaactct tctaacgtca ggcataaaac
      421 gcccatagtt atgacgaatt taccacagat tccggaaaaa tcaacctagt tacctttttt
      481 aactgccgct cagcccatgc ttcttcaata tcaaaccgag tcaccagcgc atcata
//
LOCUS       DAJTVE010000369          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000369 DAJTVE010000000
VERSION     DAJTVE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>518)
                     /locus_tag="OO439_004455"
     CDS             complement(<1..>518)
                     /locus_tag="OO439_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6318616.1"
                     /translation="VLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPPGYTG
                     MGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDAKGII
                     PEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQGGYQA
                     LVSGMNHLESFG"
BASE COUNT          131 a          142 c          124 g          121 t
ORIGIN      
        1 agccaaagct ttccagatga ttcattccgg aaaccagcgc ctggtacccg ccttgtgaaa
       61 gccctgccag cgcgcgacca tcggcatctt tacggacatt aaaacgcttg ctaatcagcg
      121 ggataatatc gttcatcagt tcgcgatctg ccgctttagc attcagcgga taaaagactt
      181 tacgtctttc ctgaggcacg aaatcttcgg gaataatgcc cttcgcatcg gtttcggtat
      241 ccgggatcac caccagcatc ggtttaattt tcccttcagc cagcaggtta tccatgattt
      301 gcgggatacg cccctgatcg atagcggaac gtccggtatc accaaaaccg tgatagaaat
      361 agagcactgg caaaggctcg cccatgccgg tgtatcctgg cggggtccag acatacatct
      421 gacgttcaga ttgcaatgcg ttggagtggt aagttatggc gatcaaatca ccatgcgcaa
      481 cagaacgcgt atccagataa ctgcctggca ccagaacc
//
LOCUS       DAJTVE010000370          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000370 DAJTVE010000000
VERSION     DAJTVE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.376"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>476
                     /locus_tag="OO439_004456"
     rRNA            <1..>476
                     /locus_tag="OO439_004456"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJTVE010000371          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000371 DAJTVE010000000
VERSION     DAJTVE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.377"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..442
                     /locus_tag="OO439_004457"
     CDS             <1..442
                     /locus_tag="OO439_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6318617.1"
                     /translation="STPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNYEEWL
                     RQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQ
                     ESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          135 a          100 c          132 g          103 t
ORIGIN      
        1 ttctacccct tacaccattt ccggtattca ggcgctggtg aaaaaaggta acgaaggcac
       61 cattaaaaca gccgatgatc tgaaaggcaa aaaagtgggt gtcggtctgg gcaccaacta
      121 tgaagagtgg ctgcggcaga atgttcaggg tgtcgatgtg cgtacctatg atgatgaccc
      181 gaccaaatat caggatctgc gcgtaggccg tatcgatgcg atcctcgttg accgtctggc
      241 ggcgctggat ctggtgaaga aaaccaacga tacgctggca gtaaccggtg aagcattttc
      301 ccgtcaggag tctggcgtgg cgctgcgtaa aggaaatgaa gatctgctga aagcagtgaa
      361 tgatgcaatt gcggaaatgc aaaaagatgg cactctgcaa gccctttccg aaaaatggtt
      421 tggtgctgat gtgaccaaat aatcagcata atgacaaaaa agggcgcttt
//
LOCUS       DAJTVE010000372          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000372 DAJTVE010000000
VERSION     DAJTVE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>465
                     /locus_tag="OO439_004458"
     CDS             <1..>465
                     /locus_tag="OO439_004458"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6318618.1"
                     /translation="IYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVN
                     EETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLR
                     TESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTP"
BASE COUNT          130 a          116 c          114 g          105 t
ORIGIN      
        1 gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag tgatttacga
       61 aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga tccacgttaa
      121 aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca ccacttctcc
      181 tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag gcaatgcagg
      241 taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag agatcaaacg
      301 tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc atatcgatac
      361 gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa acatcgataa
      421 cgagcacatg tatcttgacc cgatcaaagt caccctgctg actcc
//
LOCUS       DAJTVE010000373          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000373 DAJTVE010000000
VERSION     DAJTVE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>464
                     /locus_tag="OO439_004459"
     CDS             <1..>464
                     /locus_tag="OO439_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCR6318619.1"
                     /translation="QHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQ
                     RWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          112 a          115 c          137 g          100 t
ORIGIN      
        1 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
      121 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
      181 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
      241 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
      301 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
      361 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa agtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTVE010000374          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000374 DAJTVE010000000
VERSION     DAJTVE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.381"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..>462
                     /locus_tag="OO439_004460"
     CDS             12..>462
                     /locus_tag="OO439_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCR6318620.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          113 a          108 c          121 g          120 t
ORIGIN      
        1 cggaggccag catgaacaac aataacacat tgtacgtcgg gttagatgtt cacaaagaat
       61 cgattactgt cgcttatgct atcaattcag aacctgttga actgatgggt aaaattggca
      121 catcacctac tgatattcag aatctttgta aacgtctcag gtcaaagtca tcgcaggtca
      181 gtatcgttta tgaagcgggg ccctgtggtt atggacttta tcgtcggctg gtgaaatccg
      241 gttttgactg catggtctgc gccccctcac ttatcccgaa aaaaccgggg gagcgggtta
      301 aaaccgatcg ccgtgatgcc atcagacttg tgcgttcact gcgtgcagga gatctttctg
      361 ctgtctacgt acccggcatt gaagatgagg cattccggga tttggcccgg gcatgggcat
      421 ctgcccgcga tgatttacgg catgcaaggc aacgtctgaa at
//
LOCUS       DAJTVE010000375          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000375 DAJTVE010000000
VERSION     DAJTVE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.382"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>459)
                     /locus_tag="OO439_004461"
     CDS             complement(<1..>459)
                     /locus_tag="OO439_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6318621.1"
                     /translation="GFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRDQAVPYT
                     ATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRTEQEAKPF
                     TFEWNGRIWNAGPNSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMP"
BASE COUNT           89 a          131 c          113 g          126 t
ORIGIN      
        1 tccggcatcg acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgtttgt
       61 gctgtgacag attttgccag catgactacc ggggacaggc ggcccagtga gttggggcca
      121 gcattccaga tacgaccgtt ccattcaaac gtgaacggct tcgcctcctg ttctgtgcgc
      181 catgcttcaa tttcctgacg tctggcctct ctggccgctt ccagcatttc tggtgtcaca
      241 gtgaatgggg ctatctcacc ccatttgcca ctttgcagtt cctgccagat tcgctgaccc
      301 gtcggtgcga catcatcagc ggtggctgtg taggggactg cctggtccct gtcgtcaaaa
      361 aaaacgtcac agtctactgc gccactttcg gtataacggg gattaatgat ttttttaatt
      421 tccacggtgc attcctcacg atgtgcgaat aaaaagccc
//
LOCUS       DAJTVE010000376          455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000376 DAJTVE010000000
VERSION     DAJTVE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>455)
                     /locus_tag="OO439_004462"
     CDS             complement(<1..>455)
                     /locus_tag="OO439_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6318622.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          109 a          125 c          124 g           97 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacc
//
LOCUS       DAJTVE010000377          455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000377 DAJTVE010000000
VERSION     DAJTVE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.384"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            165..>455
                     /locus_tag="OO439_004463"
     CDS             165..>455
                     /locus_tag="OO439_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318623.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT          130 a          119 c           99 g          107 t
ORIGIN      
        1 tttcgcgcaa tgggtgatag aaaatcgcgc caagtgataa tgcttatcaa aattattatc
       61 acttgcatga acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
      121 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
      181 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca
      241 cgcctgtggc tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg
      301 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
      361 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
      421 agggcgcaac caaccttacc gacgcgctca aaaac
//
LOCUS       DAJTVE010000378          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000378 DAJTVE010000000
VERSION     DAJTVE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.385"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>452)
                     /locus_tag="OO439_004464"
     CDS             complement(<1..>452)
                     /locus_tag="OO439_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6318624.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           75 a          145 c          128 g          104 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca ga
//
LOCUS       DAJTVE010000379          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000379 DAJTVE010000000
VERSION     DAJTVE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.386"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>447
                     /locus_tag="OO439_004465"
     CDS             <1..>447
                     /locus_tag="OO439_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318625.1"
                     /translation="AGGGILEATTLITALADKYKQTQTPRNLSIISPTGLGDRADRGI
                     SPLAQEGLVKWALCGHWGQSPRISELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIIS
                     DIGIGTFVDPRQQGGKLNEVTKEDLIKLVEFDNKEYLYYKAIAPDI"
BASE COUNT          130 a          104 c          105 g          108 t
ORIGIN      
        1 gcgctggcgg cggtattctg gaagccacca cgttaattac agctcttgct gataaatata
       61 aacagactca aacgccacgt aatttatcga ttattagtcc gacagggctt ggcgatcgcg
      121 ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt gaaatgggca ttatgtggtc
      181 actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt attgcttata
      241 actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac cagcctggta
      301 ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc ggcaaactga
      361 atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa gaatatctct
      421 attacaaagc gattgcgcca gatatcg
//
LOCUS       DAJTVE010000380          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000380 DAJTVE010000000
VERSION     DAJTVE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.387"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>444
                     /locus_tag="OO439_004466"
     CDS             <1..>444
                     /locus_tag="OO439_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6318626.1"
                     /translation="FTLGFILLAVCGTSLFTSSVMTVMAKSRGVISWRTWLINALLVA
                     CGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTFTESVSLGIMCNLMVC
                     LALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPFAIA"
BASE COUNT           80 a           95 c          126 g          143 t
ORIGIN      
        1 tttacactcg gctttatttt gctggcggtt tgtggcacca gcctgttcac ctcgtcggta
       61 atgacggtga tggcaaaaag tcggggcgtt attagttggc gaacttggct gattaacgca
      121 cttctggtgg cctgcggtaa tctggcaggt attgcctgtt tcagtttgtt aatctggttt
      181 tccgggctgg tgatgagtga aaacgcgatg tggggagtcg cggttttaca ctgcgccgag
      241 ggcaaaatgc atcatacatt tactgaatct gtcagcctcg gcattatgtg caatctgatg
      301 gtttgtctgg cgctgtggat gagttattgc gggcgttcat tatgcgacaa aatcgtcgcc
      361 atgattttgc ccatcaccct gtttgtcgcc agtgggtttg agcactgtat cgccaatttg
      421 tttgtgattc cgttcgccat tgcc
//
LOCUS       DAJTVE010000381          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000381 DAJTVE010000000
VERSION     DAJTVE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.388"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..>442
                     /locus_tag="OO439_004467"
     CDS             35..>442
                     /locus_tag="OO439_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCR6318627.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRS
                     TDIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYL
                     KVKDFGIVSGSPIKQAKHLSYESVLKNLLSAKIL"
BASE COUNT          141 a           69 c           93 g          139 t
ORIGIN      
        1 aaatagcgta gtgtgacaaa aataagatga atttatgaaa aaaaacattg ttagacaagt
       61 gcttgagagt tatggtccgt gcatatcttc tgaacttgct gagaggatta agcgtcagaa
      121 tccttctatg tcatctgatg caatacgtaa aatgatctct cgaagcactg atattggtaa
      181 gttgccattt ttaagattta gtcataacag gagattcatt tatctaaaag atgactttgg
      241 tagcttcaaa ttttggcggg cgctcaaaaa atgtatgcgt gaggcaaatt cggcgtattc
      301 tcacgctata ctttccgtaa ttaataatgg cggatacttg aaggtaaagg atttcgggat
      361 tgtgtctggc tctccgataa agcaggccaa gcatttaagt tatgaatctg ttctaaaaaa
      421 tctgctttca gctaaaatac tt
//
LOCUS       DAJTVE010000382          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000382 DAJTVE010000000
VERSION     DAJTVE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.389"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..>441
                     /locus_tag="OO439_004468"
     CDS             8..>441
                     /locus_tag="OO439_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6318628.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGF"
BASE COUNT          105 a          100 c          125 g          111 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg a
//
LOCUS       DAJTVE010000383          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000383 DAJTVE010000000
VERSION     DAJTVE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.390"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(148..>437)
                     /locus_tag="OO439_004469"
     CDS             complement(148..>437)
                     /locus_tag="OO439_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6318629.1"
                     /translation="GSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFF
                     MVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          133 a          106 c           97 g          101 t
ORIGIN      
        1 accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct tatccggtct
       61 acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca tatcgcatca
      121 ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg caccgaccgg
      181 aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca gatgaatctg
      241 gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa tcagcgttac
      301 catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata gggcttgcgt
      361 agactgttca acattcggta catagccaat catccctaac acaaccgcaa tgaaaaaagc
      421 cgcaaaggct gaaccgc
//
LOCUS       DAJTVE010000384          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000384 DAJTVE010000000
VERSION     DAJTVE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.391"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..354)
                     /locus_tag="OO439_004470"
     CDS             complement(<1..354)
                     /locus_tag="OO439_004470"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6318630.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMR"
BASE COUNT          121 a          109 c           92 g          106 t
ORIGIN      
        1 acgcatacga atggcgatat cttccgcctg ccccagcgca tattcaaaaa accagagcgc
       61 catccgcata tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag
      121 cgggagatat tcattaagtt gattgatatc gctgcataaa tcgagactgt actcatccca
      181 gtcaaaaatc acgccacaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct
      241 gttttgtggc cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat
      301 gggctcatct ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc
      361 ctcctggaaa atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca
      421 ccgaagtg
//
LOCUS       DAJTVE010000385          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000385 DAJTVE010000000
VERSION     DAJTVE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.392"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            43..219
                     /locus_tag="OO439_004471"
     CDS             43..219
                     /locus_tag="OO439_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318631.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          153 a           48 c           99 g          126 t
ORIGIN      
        1 agtcattacc tataaggggt acgaataaag taacgggggg cagtgagttt tatttttaat
       61 gaggaaacat tgtattttac tgtttttggt ggggatttta ttatggatga tttaaaaata
      121 gtaaaaaata cggaagcatg ggtatggtat aaaaattgcg atagtaaaaa taaaaaagtg
      181 gcagttgaac ttcttcaaat ctacgaattg catcaatgaa aaatatagtt aaatagagga
      241 ggttattaat agcaggaaaa gcatcacgtg atgctgattt tttgcactaa ataattcgcg
      301 ctaatgttca atagttaata tatccctcat cagggaaaga tcacggtgtg ccgtaggcac
      361 aaagatgcaa aacgggacgt tttgcatgta acatgtaata agcataagct tgtgaagaga
      421 tggtgg
//
LOCUS       DAJTVE010000386          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000386 DAJTVE010000000
VERSION     DAJTVE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.393"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          148 a           78 c           73 g          118 t
ORIGIN      
        1 atattaatcg tatgcaggaa gataccttga ttagctcagc ttatccagag gaataaacaa
       61 tgttagagct actaatattt attgccacac atggcaatct attaatcttt atctagtatg
      121 acgattcaac tatgaatagt cgaatgctac gtgaaatctt ctgaacagat accaacatca
      181 aaaattagat ttcacgtagc tcacgcttaa cagaatagga ataacaacaa tgtttgacac
      241 aaagcaaatt agatactcag gtaaagatgg tatctatttt caccttagag ataatgtgga
      301 atcttttctt gccttaacag ctactgaaca gatggactat gactcaccga tgatggtgac
      361 aactcaggct atgcaatcgg ggcaaactat cactgataat gttcagagag cacctaa
//
LOCUS       DAJTVE010000387          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000387 DAJTVE010000000
VERSION     DAJTVE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.394"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..406)
                     /locus_tag="OO439_004472"
     CDS             complement(<1..406)
                     /locus_tag="OO439_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6318632.1"
                     /translation="MTQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGA
                     KPSKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKP
                     NLTCMPQDDPQYPGMETKTASDYLPQTNWSTTQ"
BASE COUNT           97 a           97 c           92 g          130 t
ORIGIN      
        1 attgagttgt tgaccaattt gtctgtggca ggtaatcact tgctgtcttt gtctccatcc
       61 ctggatactg gggatcatcc tgcggcatac aagtcagatt gggttttacc cacttcctgc
      121 aatacagtgt gtaatcgtaa gggcttaatg ctctgaagaa tgttttgaaa acattaggcg
      181 cactactggc ctggtcaata ggatatggca gtcttaattg tttcgctatg tctggcaact
      241 tacttggatc gggcgtaggc ggtttgctgg gtttcgctcc atatccgcca ttatttaagt
      301 gtccatattt agatgccagc cccattgaat ctatatactg aaccggattc aacggatact
      361 gataaaaatt ccatcccccc ttcagtccaa tcggatcctg agtgatatac cgcccc
//
LOCUS       DAJTVE010000388          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000388 DAJTVE010000000
VERSION     DAJTVE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.395"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..364)
                     /locus_tag="OO439_004473"
     CDS             complement(<1..364)
                     /locus_tag="OO439_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR6318633.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDAL"
BASE COUNT          118 a           80 c           82 g          128 t
ORIGIN      
        1 ccagagcatc ggttaccttt tcttccagcc cataacctac ctggatgcgg acagtacgat
       61 ctgaccaggc aacaatgatc agtatcccat cattacgttt ggcatctcct aaacgccaat
      121 tgtcaaaaac tcttgtagca tattgttcaa ttgtctcgtc tttggttgtg ggaacaatta
      181 atacagcaag ttgcacttgg gtattattga aaatagattc taacttgtgc gtcagttctt
      241 tcttttcttg tgagtttaag ctactcgtaa gatccgtaac ttgttgctgt aattgtggga
      301 caatagttgg ttgggctgca acaaaggaac acacgaaagt gaacattagt atgaaatagc
      361 gcattataaa tttctcatta aatctggata aataaattga cggcggca
//
LOCUS       DAJTVE010000389          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000389 DAJTVE010000000
VERSION     DAJTVE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.396"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            48..>406
                     /gene="mhpC"
                     /locus_tag="OO439_004474"
     CDS             48..>406
                     /gene="mhpC"
                     /locus_tag="OO439_004474"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6318634.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSS"
BASE COUNT          102 a           97 c          120 g           87 t
ORIGIN      
        1 ggctcgttaa gcgccagaac agagaactga atatgcagga gaagatgatg agttatcagc
       61 cacaaaccga agccgccacc agccgttttc tgaatgtaga agaagcgggt aaaacgctgc
      121 gcatccattt taatgactgc ggacaaggcg acgaaaccgt tgtcctgctg catggttccg
      181 gcccgggtgc tactggctgg gcgaacttca gccgcaatat cgatccgctg gtagaggcgg
      241 gctatcgggt gatcctgctg gattgtccgg gttggggcaa aagcgattcg atcgttaata
      301 gtggttcacg atcggatctt aatgcacgaa tcctgaaaag cgtggtggat caactggata
      361 tcgccaaaat ccacctgctg ggcaactcga tgggcggcca tagttc
//
LOCUS       DAJTVE010000390          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000390 DAJTVE010000000
VERSION     DAJTVE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.397"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>404)
                     /gene="csgD"
                     /locus_tag="OO439_004475"
     CDS             complement(<1..>404)
                     /gene="csgD"
                     /locus_tag="OO439_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6318635.1"
                     /translation="SGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPY
                     RDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTES
                     ALLTHREKEILNKLRIGASNNEIARSLFISEN"
BASE COUNT          108 a           88 c           88 g          120 t
ORIGIN      
        1 attttcgctg atgaacaacg aacgagcgat ctcgttatta gacgcgccga tacgcagttt
       61 attcaggatc tctttttccc gatgagtaag gagggctgat tccgtgctgt tataacgata
      121 gttacctgaa tgcgtaatca agtagctggc aagcttttgc gtaaagtagc attcgccgcg
      181 caggacgcct tgcaacccat tgacaacacg ttcttgatcc tccatggcat aaaaaacgcc
      241 gttgatatga ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg tatttagcaa
      301 caatattttg atattgttgt ttttcctgct caaagtatcc tgccaataat ggataagctt
      361 tttatccgct tccatcatat ccagaagaat aatagagcct gaag
//
LOCUS       DAJTVE010000391          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000391 DAJTVE010000000
VERSION     DAJTVE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.398"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..354)
                     /locus_tag="OO439_004476"
     CDS             complement(<1..354)
                     /locus_tag="OO439_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6318636.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLEL"
BASE COUNT          113 a           97 c          103 g           90 t
ORIGIN      
        1 caattcaagg cgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg
       61 caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga
      121 gatatacagc cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa
      181 ctgatgcggt ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac
      241 cttcttgccg tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa
      301 aacggatacc ggcaaacata atgcaattaa ggtggtcacg atgccggttt tcataggtag
      361 ctcctgtcaa aagaccgccc gaataataat gtgtcaaaat ttt
//
LOCUS       DAJTVE010000392          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000392 DAJTVE010000000
VERSION     DAJTVE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.399"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          119 a           78 c           61 g          135 t
ORIGIN      
        1 gaacccaaga acatgtgacc cagagcgaag cgacaaggtt ttaggttaaa aagcatactc
       61 tgttttaaca aaaagtgcta tatttaataa atagcacgaa ttgctaaaga caaaggtatt
      121 gacaaactgc tagtattgag acatagccct taattaatag atactttata ggctttactt
      181 aaacagtctg ttcaaacggt caacggtcac ttcctctggt tcttcgttcc tctcttcttg
      241 gaagatatcc tttaagactt ttaagttatc tattaagaca gtctattaag actcctactt
      301 aataagggac tttccttcgg ttctttgtcc ctctcttctt atcttagtgg ttctttacag
      361 taacttctaa gaatctctta atacccttta tag
//
LOCUS       DAJTVE010000393          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000393 DAJTVE010000000
VERSION     DAJTVE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.400"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>391
                     /locus_tag="OO439_004477"
     CDS             <1..>391
                     /locus_tag="OO439_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6318637.1"
                     /translation="WSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT           91 a          109 c          121 g           70 t
ORIGIN      
        1 tggagccgtt acggtcagtt gctgaccgtc actgactgtt ccggttatgt aacccgttat
       61 gaccatgacc gttttggtca ggtgacggcg gtgcaccgcg aggaagggct gagccagtac
      121 cgcgcatacg acagccgtgg acagttaatt gcggtgaaag acacgcaggg ccatgaaatg
      181 cggtatgaat acaacgccgc cggtgacctg accgccgtca ttgccccgga cggcagcaga
      241 aacgggacac agtacgatgc gtggggaaaa gccatctgta ccacgcaggg cgggctgacg
      301 cgcagtatgg aatacgatgc tgccggacgg gtcatccgtc tgaccagtga aaacggcagc
      361 cacaccacct tccgttacga tgtactcgac c
//
LOCUS       DAJTVE010000394          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000394 DAJTVE010000000
VERSION     DAJTVE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.401"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>389)
                     /locus_tag="OO439_004478"
     CDS             complement(<1..>389)
                     /locus_tag="OO439_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR6318638.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          134 c          114 g           70 t
ORIGIN      
        1 cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc
       61 ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc tcacaccagc
      121 cgagcagcca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc ggacttaagc
      181 ggagttcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca tccagtttcg
      241 ccacgccgcc gcgcaccagc cagagtgatt cgctgcggct gtaaccgtcc tcaccgggaa
      301 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
      361 gcagctgtaa gcggatatcc gcaggcatt
//
LOCUS       DAJTVE010000395          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000395 DAJTVE010000000
VERSION     DAJTVE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.402"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(135..>389)
                     /locus_tag="OO439_004479"
     rRNA            complement(135..>389)
                     /locus_tag="OO439_004479"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           82 a          114 c           90 g          103 t
ORIGIN      
        1 gcatggggtc aggtgggacc accgcgctac ggccgccagg caaattctgt tttatcagac
       61 cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac
      121 atcttcggcg ttgtaaggtt aagcctcacg gttcattagt accggttagc tcaacgcatc
      181 gctgcgctta cacacccggc ctatcaacgt cgtcgtcttc aacgttcctt caggaccctt
      241 aaagggtcag ggagaactca tctcggggca agtttcgtgc ttagatgctt tcagcactta
      301 tctcttccgc atttagctac cgggcagtgc cattggcatg acaacccgaa caccagtgat
      361 gcgtccactc cggtcctctc gtactagga
//
LOCUS       DAJTVE010000396          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000396 DAJTVE010000000
VERSION     DAJTVE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.403"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..353)
                     /locus_tag="OO439_004480"
     CDS             complement(<1..353)
                     /locus_tag="OO439_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6318639.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSA"
BASE COUNT          102 a          117 c           85 g           83 t
ORIGIN      
        1 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
       61 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      121 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      181 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
      241 tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc caccgcaacg
      301 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
      361 acgaccgcat cccctaaaac ccatact
//
LOCUS       DAJTVE010000397          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000397 DAJTVE010000000
VERSION     DAJTVE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.404"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..350)
                     /locus_tag="OO439_004481"
     CDS             complement(<1..350)
                     /locus_tag="OO439_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6318640.1"
                     /translation="MSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKP
                     WYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYREHDQKL
                     AREGGLDLVVLGLGA"
BASE COUNT           93 a           97 c           87 g          105 t
ORIGIN      
        1 gcacctaatc ccagcaccac caaatccagg ccgccttcac gcgccagttt ctgatcatgc
       61 tcgcggtagt tatcaatagt gagcttctgg atattctctt ctttgatccc cgcaggggtg
      121 aaaaacagat tacgcagatt ggtaatcgtt acgccttctc cctctttgcc gcgaaaggga
      181 atttcatcga aattatagaa atagcagtta tcgtaccagg gcttaccttt aaccagggtg
      241 gtgaggtatt catacatgcc tttgggcgtg ctaccggcgg taattgccag gttaacacga
      301 cgcgtcttcg acatataacc aagcagatgg tgtgccgcga cacggctcat ttcctggtaa
      361 tcttcggtaa tgattaattt ca
//
LOCUS       DAJTVE010000398          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000398 DAJTVE010000000
VERSION     DAJTVE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.405"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..>380
                     /locus_tag="OO439_004482"
     CDS             74..>380
                     /locus_tag="OO439_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6318641.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     "
BASE COUNT           87 a           93 c          119 g           81 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagctgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg
//
LOCUS       DAJTVE010000399          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000399 DAJTVE010000000
VERSION     DAJTVE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.406"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..340
                     /locus_tag="OO439_004483"
     CDS             <1..340
                     /locus_tag="OO439_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6318642.1"
                     /translation="LVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQI
                     DNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSG
                     YLTLKSLQRQ"
BASE COUNT           65 a           84 c          113 g          116 t
ORIGIN      
        1 gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg caggcggctg
       61 gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg cactggcagc
      121 aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc tggggggatt
      181 aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc ctgcatggtt
      241 tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta cgcgggtgtg
      301 gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt atttgctact
      361 gtccgggccg cgttcacc
//
LOCUS       DAJTVE010000400          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000400 DAJTVE010000000
VERSION     DAJTVE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.407"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>377
                     /locus_tag="OO439_004484"
     CDS             <1..>377
                     /locus_tag="OO439_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6318643.1"
                     /translation="SLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSLNDT
                     QKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGRINS
                     RSTGSVEGGKSRLSVADAITRVM"
BASE COUNT           98 a           61 c          106 g          112 t
ORIGIN      
        1 agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt cgattcacta
       61 accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc ttatcgttca
      121 ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt aagtgcatta
      181 aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg tggtatctgg
      241 aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc aggaagaatc
      301 aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt agctgatgca
      361 attaccagag taatgat
//
LOCUS       DAJTVE010000401          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000401 DAJTVE010000000
VERSION     DAJTVE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.408"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>370
                     /locus_tag="OO439_004485"
     CDS             <1..>370
                     /locus_tag="OO439_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6318644.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQH"
BASE COUNT           88 a           97 c          103 g           82 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac
//
LOCUS       DAJTVE010000402          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000402 DAJTVE010000000
VERSION     DAJTVE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.410"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..>364
                     /locus_tag="OO439_004486"
     CDS             54..>364
                     /locus_tag="OO439_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6318645.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     T"
BASE COUNT           96 a           95 c           94 g           79 t
ORIGIN      
        1 cactttatgc gtaaaatggt aaacatctac cgaggagggg caagggagta ttcatgagta
       61 tcatcgaagt gacaggtaat ccacgccatg accaactcgt tcatctaatc gcagaacgcg
      121 gttatatgaa tattgaagaa ctggcacaac tgctggatgt ttcaacgcaa accgtgcgcc
      181 gggatatccg aaaactcagt gagcagggct tgatcacccg tcatcatgga ggggccgggc
      241 gcgtctccag cgtcatgaat accgcttttg agcaacggga actttcgctc accgccgaga
      301 aacgggcgat cgctgaggca gtcgccgatt accttcccga acgctgtacc gtctttatca
      361 ccat
//
LOCUS       DAJTVE010000403          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000403 DAJTVE010000000
VERSION     DAJTVE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.411"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            87..194
                     /locus_tag="OO439_004487"
     CDS             87..194
                     /locus_tag="OO439_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6318646.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           95 a           87 c          103 g           76 t
ORIGIN      
        1 acctgacaac accacggggg ccattcttgc atccaaacaa tacaagaata gccgcgaaac
       61 gttgtcatta caatacaggc ggctatatga cgctcgcaga actgggcatg gccttctggc
      121 atgatttagc ggctccggtc attgctggta ttcttgccag tatgatcgtg aactggctga
      181 acaagcggaa gtaacgtgcc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg
      241 atcaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
      301 gcggataata ttctgcggaa tgcgcagcac agagtgaaga cgtgacggat tccggaacag
      361 a
//
LOCUS       DAJTVE010000404          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000404 DAJTVE010000000
VERSION     DAJTVE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.412"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>361
                     /gene="mhpC"
                     /locus_tag="OO439_004488"
     CDS             <1..>361
                     /gene="mhpC"
                     /locus_tag="OO439_004488"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6318647.1"
                     /translation="LNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQPTIE
                     NLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAE
                     IKAQTLIVWGRNDRFVPM"
BASE COUNT           83 a           93 c          102 g           83 t
ORIGIN      
        1 ccttaactgg ccggagcgcg tcggcaaact ggtgctgatg ggcggcggta cgggcggcat
       61 gagtttgttt acgccgatgc caaccgaagg tattaagcga ctgaatcagc tttatcgtca
      121 gccgactatc gaaaacctga agctgatgat ggatatcttc gttttcgata ccagcgattt
      181 gaccgacgcc ctgtttgaag cgcgcctgaa taatatgctg tcgcgccgcg atcacctgga
      241 aaacttcgtt aagagcctgg aagctaatcc gaaacagttc ccggattttg gcccacgtct
      301 ggcggaaatc aaagcgcaaa ccctgattgt ctgggggcgc aacgaccgct ttgtgccgat
      361 g
//
LOCUS       DAJTVE010000405          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000405 DAJTVE010000000
VERSION     DAJTVE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.413"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>360)
                     /locus_tag="OO439_004489"
     CDS             complement(<1..>360)
                     /locus_tag="OO439_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6318648.1"
                     /translation="IITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPGAVDFIE
                     GFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAASAAVSI
                     GNARNVRAFFTDAPPPN"
BASE COUNT           98 a           92 c           79 g           91 t
ORIGIN      
        1 aattgggagg cggggcatca gtaaaaaaag ccctgacatt ccgtgcattg ccaattgaaa
       61 cggcagcaga cgcggcaaac ttcgtatgat cggccactaa caatgtattc cgcgcatgtt
      121 taatcatcgt tctcgcgact aacgcttcat ttacatcaaa ttccagtagg gtgccgtcat
      181 gttcaatggc acctatactg gtgattaaat aatctgcgcg gaagccttca ataaaatcca
      241 cggctcctgg gccgataatc ccgccgttat gagcgcgtaa agtgcctccc ggcaccatca
      301 cttcaatatc ctgattctta taaagaatct gtgccacacg taggctgtta gtgataatgc
//
LOCUS       DAJTVE010000406          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000406 DAJTVE010000000
VERSION     DAJTVE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.414"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..99
                     /locus_tag="OO439_004490"
     CDS             <1..99
                     /locus_tag="OO439_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6318649.1"
                     /translation="HTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ"
     gene            complement(100..291)
                     /locus_tag="OO439_004491"
     CDS             complement(100..291)
                     /locus_tag="OO439_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6318650.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
BASE COUNT          107 a           89 c           78 g           85 t
ORIGIN      
        1 catacaccgt tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct
       61 gtctttgggg gcgaactgac tcttgtgcaa ttgcaataat taaaaatcac cgtactcctg
      121 tgcaggtcgc cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat
      181 ccgttgatcg ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat
      241 atcttcacaa ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc
      301 gaagtgtggt gccgtaccgt taagtttagg agagatttta caacggggga ataaccagg
//
LOCUS       DAJTVE010000407          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000407 DAJTVE010000000
VERSION     DAJTVE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.415"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           99 a           76 c           92 g           91 t
ORIGIN      
        1 ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt
       61 ttttgaagga aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta
      121 aacgtatctc aggcgagcgc attgcgccgt tctgacgcag ataaactagc ctggatagat
      181 ggtgctggca ataaaaaata gcgttttctt atcggtgtcg gtaagattgc tgcgggtgct
      241 tgaggctgtc tgcctcgggt atgtcactgt aaggcagaca gagaaaagcc ccagttaaca
      301 ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaat
//
LOCUS       DAJTVE010000408          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000408 DAJTVE010000000
VERSION     DAJTVE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.416"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>356)
                     /locus_tag="OO439_004492"
     CDS             complement(<1..>356)
                     /locus_tag="OO439_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6318651.1"
                     /translation="LFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGT
                     DTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETF
                     GERIQPLMQCRAHLPAL"
BASE COUNT           76 a          100 c           97 g           83 t
ORIGIN      
        1 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
       61 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      121 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
      181 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
      241 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
      301 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaa
//
LOCUS       DAJTVE010000409          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000409 DAJTVE010000000
VERSION     DAJTVE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.417"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(260..>353)
                     /locus_tag="OO439_004493"
     CDS             complement(260..>353)
                     /locus_tag="OO439_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HCR6318652.1"
                     /translation="ETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          112 a           81 c           72 g           88 t
ORIGIN      
        1 ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt caagtaatga
       61 ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag tgtacttctg
      121 atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg cacagatttg
      181 taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg ccggatgcgg
      241 cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc cgcgcgccca
      301 aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt tcc
//
LOCUS       DAJTVE010000410          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000410 DAJTVE010000000
VERSION     DAJTVE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.418"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           89 a           78 c           50 g          118 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag
      301 tttctgtcca tggctgcttt tcgcatctta cgtct
//
LOCUS       DAJTVE010000411          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000411 DAJTVE010000000
VERSION     DAJTVE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.419"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..>334
                     /gene="istB"
                     /locus_tag="OO439_004494"
     CDS             74..>334
                     /gene="istB"
                     /locus_tag="OO439_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCR6318653.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQM"
BASE COUNT           96 a           82 c           94 g           62 t
ORIGIN      
        1 catccggcat atgcttcaga gcatgaaaac ctgcgtggtc ctggctacta ccactgacag
       61 aaggaacaac cgtatgagtg ataatctgtt aaacaaactg acccaactga aactcccggc
      121 gatggccggg agcctgatac ggcaacggga aacgcctcag acttacgacg aactgtcgtt
      181 cgaggaaagg ctgacactgc tggttgatga cgagcttctg agcagagaaa acagtcgtgt
      241 ggcacggttg agaaaaaatg cctgcctgaa atatcaggca acaccggaag ggctgcgtta
      301 tccggcctca agaggtctgc gggctgaaca gatg
//
LOCUS       DAJTVE010000412          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000412 DAJTVE010000000
VERSION     DAJTVE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.420"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>333
                     /locus_tag="OO439_004495"
     CDS             <1..>333
                     /locus_tag="OO439_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318654.1"
                     /translation="LDEPLSTTRLNHLIDKGYERITLQLDLGGESPGYLEKDKHYREA
                     DAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQSANFWFNDIKTD
                     TDQNSHAK"
BASE COUNT          131 a           54 c           66 g           82 t
ORIGIN      
        1 actggatgaa cctctttcaa caacgaggtt gaaccacctc atagataaag gttatgaaag
       61 aataacgtta cagcttgatt tagggggaga atccccaggt tatctggaaa aagataaaca
      121 ttacagagaa gccgatgcgg ctttacttaa tgttatttat cctgcaaatc ttgcaaagat
      181 aaacaccaga agaaaagagc aggttctaaa aatagttaaa aaactagcag gtccgtatgg
      241 tataaaaaga tacgagaagg ataattatca gtcggcaaat ttttggttca atgatattaa
      301 aaccgatacc gatcaaaatt cacatgcgaa aag
//
LOCUS       DAJTVE010000413          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000413 DAJTVE010000000
VERSION     DAJTVE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.421"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           87 a           68 c           46 g          130 t
ORIGIN      
        1 gtaacctctc gttacatcca tttgtcacat agcactctgt gtaatgtctt tctatgtgtg
       61 tgccataatc cccctatatt ccctgtgtaa atcatagaat aatgctctct attccctgtg
      121 taattcccta ctaaaaaatc caatgaaaag tagcttaaaa gttcaattct tactttcttc
      181 acttttacat cactttcgca aagccttgtg taatgcggct tacagagcac catcagaaat
      241 tgttggctaa aaattgttct gtttgtttct gaacgagttt ctcgtttgtg caaattttaa
      301 aatttatttt gtttttatag ccttatttcg t
//
LOCUS       DAJTVE010000414          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000414 DAJTVE010000000
VERSION     DAJTVE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.422"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>320
                     /locus_tag="OO439_004496"
     CDS             <1..>320
                     /locus_tag="OO439_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6318655.1"
                     /translation="LNRDDTGAPKTVVLGGATRLRVSSQSLKRAWRTSALFEQALAGH
                     IGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAKNDKKPKDPLTNAETEQL
                     VHISP"
BASE COUNT           93 a           72 c           85 g           70 t
ORIGIN      
        1 cttaaccgtg atgacaccgg tgcgccaaaa actgtggtgc tgggtggagc aacgcgtctg
       61 cgcgtttcct cgcaaagtct gaaacgtgcg tggcgcactt ctgcactttt tgaacaggca
      121 ctggcgggcc atattggtat tcgcagtggg cgtattgcgc gtgaggcggc aactatcctg
      181 attgagaaag ggattgaaga taaaaaagcc atcgaatggg cggcaaaaat tgctgattat
      241 cttgggaaag ctaaaaatga caaaaaacca aaagatccgc tcactaacgc cgaaactgaa
      301 caattagttc atatcagccc
//
LOCUS       DAJTVE010000415          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000415 DAJTVE010000000
VERSION     DAJTVE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.423"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>318
                     /locus_tag="OO439_004497"
     CDS             <1..>318
                     /locus_tag="OO439_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318656.1"
                     /translation="LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPP"
BASE COUNT           64 a          105 c           81 g           68 t
ORIGIN      
        1 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
       61 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      121 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
      181 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
      241 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc
      301 attacggtca atccgcct
//
LOCUS       DAJTVE010000416          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000416 DAJTVE010000000
VERSION     DAJTVE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.425"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO439_004498"
     tRNA            71..146
                     /locus_tag="OO439_004498"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJTVE010000417          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000417 DAJTVE010000000
VERSION     DAJTVE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.426"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>313
                     /locus_tag="OO439_004499"
     CDS             <1..>313
                     /locus_tag="OO439_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6318657.1"
                     /translation="IYQRNALPAITGHICDHQCQYNCTRLDYDSALNIRELKKVALEK
                     GWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAGYFLARAGHPVTLFEREANAGGVVK
                     NI"
BASE COUNT           76 a           86 c           85 g           66 t
ORIGIN      
        1 atctaccaac gcaacgccct acctgccatt accggtcata tttgcgatca ccagtgccaa
       61 tacaactgta cccgcctgga ttacgacagt gcgctgaata tccgcgaact gaaaaaagtc
      121 gcgctggaaa aaggttggga tgaatataag caacgctggc acaaacccgc cggttctggt
      181 tcacgccatc cggttgccgt gattggtgca ggtccggcgg gtctggcagc aggttacttc
      241 cttgccagag cgggccatcc ggttacgctg tttgaacgcg aagccaatgc tggcggcgtg
      301 gtgaaaaata tca
//
LOCUS       DAJTVE010000418          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000418 DAJTVE010000000
VERSION     DAJTVE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.427"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..>312
                     /locus_tag="OO439_004500"
     CDS             35..>312
                     /locus_tag="OO439_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HCR6318658.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGV"
BASE COUNT           82 a           61 c           74 g           95 t
ORIGIN      
        1 tatagtttgc catatttata tttaaggaat tgttatggga aataatttgt tatcagcaaa
       61 agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc atttaggctt
      121 tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt
      181 cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt
      241 acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg
      301 cgtcgttggc gt
//
LOCUS       DAJTVE010000419          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000419 DAJTVE010000000
VERSION     DAJTVE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.428"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           94 a           70 c           58 g           90 t
ORIGIN      
        1 ttaacgttac taataaatcg tgaaacaatc tcctcatttg ttagtatttt cgcctgagca
       61 cagcaagagg ttaatattgc aaccgtcatt aaaaatggct ttatcacaaa acaaactcca
      121 ttgttagcat gttacgcgat aattttgcct catctttcac cacaaaaaaa gaactcacct
      181 tttaaatgga gaatacccaa catcatgggt gattttcata aagggatcgc ctgttttcgg
      241 caaatcgata acgcgcattc caggcggcgc gttgtcaaac gtcaccttaa gttcgctgcg
      301 ggttgccggt ga
//
LOCUS       DAJTVE010000420          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000420 DAJTVE010000000
VERSION     DAJTVE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.429"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..>309
                     /gene="mpaA"
                     /locus_tag="OO439_004501"
     CDS             30..>309
                     /gene="mpaA"
                     /locus_tag="OO439_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6318659.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANAN"
BASE COUNT           57 a           93 c           84 g           75 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatg
//
LOCUS       DAJTVE010000421          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000421 DAJTVE010000000
VERSION     DAJTVE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.430"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>308
                     /locus_tag="OO439_004502"
     CDS             <1..>308
                     /locus_tag="OO439_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6318660.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJTVE010000422          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000422 DAJTVE010000000
VERSION     DAJTVE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.denovo.431"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..235
                     /locus_tag="OO439_004503"
     CDS             <1..235
                     /locus_tag="OO439_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6318661.1"
                     /translation="KAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLAAQEAL
                     STAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
BASE COUNT           69 a           68 c           88 g           78 t
ORIGIN      
        1 gaaagcgcaa agggaaaaaa ttcagaaact gatccctgcg gttctggcaa ttcttccgcg
       61 tgagctgcgt caccgactct gtatcttcga taccctggaa cgccgtgcat tactggcggc
      121 gcaggaagcg ttgagtacgg caattgatgc gcatgatgat gctgtccagg ccgtttaccg
      181 gaaagcacat ttcagcggtg gtgggtcgcc cggcgattct gtcgtagtgc attgattgaa
      241 attaatcgtg ccggattgtt ttgttcggta tcagttaaat gtaacgctgc gagcgttaca
      301 agg
//
LOCUS       DAJTVE010000423         7203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000423 DAJTVE010000000
VERSION     DAJTVE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.guided.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..277
                     /locus_tag="OO439_004504"
     CDS             <1..277
                     /locus_tag="OO439_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HCR6318662.1"
                     /translation="DMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALM
                     IANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            277..1113
                     /locus_tag="OO439_004505"
     CDS             277..1113
                     /locus_tag="OO439_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6318663.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1220..1624)
                     /locus_tag="OO439_004506"
                     /pseudo
     CDS             complement(1220..1624)
                     /locus_tag="OO439_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1974..2405)
                     /locus_tag="OO439_004507"
     CDS             complement(1974..2405)
                     /locus_tag="OO439_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HCR6318664.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2473..5184)
                     /locus_tag="OO439_004508"
     CDS             complement(2473..5184)
                     /locus_tag="OO439_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR6318665.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(5196..5528)
                     /locus_tag="OO439_004509"
     CDS             complement(5196..5528)
                     /locus_tag="OO439_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6318666.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            5761..6096
                     /locus_tag="OO439_004510"
     CDS             5761..6096
                     /locus_tag="OO439_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCR6318667.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(6182..7030)
                     /locus_tag="OO439_004511"
     CDS             complement(6182..7030)
                     /locus_tag="OO439_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6318668.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         1722 a         2022 c         1648 g         1811 t
ORIGIN      
        1 cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac
       61 tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga
      121 tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg
      181 cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc
      241 cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct
      301 gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg
      361 aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac
      421 attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc
      481 cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc
      541 tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg
      601 gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat
      661 cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac
      721 tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg
      781 ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata
      841 gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg
      901 gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc
      961 tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc
     1021 ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc
     1081 gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg
     1141 ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc
     1201 ccttcccgca aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact
     1261 gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc
     1321 gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct
     1381 cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga
     1441 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
     1501 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
     1561 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
     1621 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
     1681 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
     1741 cctgccagac atgctgatta attttgccct tattaacatc acagacagaa agaacgaggg
     1801 taccaatacc attgatggta actggcaggc cgatgaagga cgccgttacc gggataatgt
     1861 aagaatctat ttttgagtat gtcaacataa cctgaggcaa agtacctcag gttccagtgt
     1921 agtaatcagc caacctgaca gtcttctgtt agccctacag atatgtcgtg gagctaaccg
     1981 cgcatgtcaa agccggattt ttgaaaatct ctataaactt ccggattgtt gaaggccggg
     2041 aatttagctt tatgggctgc tgactttatc gcttcgcaat aggctttatc gccattactg
     2101 gttgatattt ttaacgccgt gccatcccgg gagaattcca tatgcaacct gcattttttc
     2161 cttttccatt tttgcggatc atcaagttta gcattaattg catccctgat tccccgtgct
     2221 tgctcccccc attcatcctg atcatcccaa cgtcctgaac tgcaactacc cagggctgta
     2281 gttttatggc aatttgaagg atgtaatggc gcacatcccg ccacaaagct gactaaaata
     2341 gccagcataa cgattttctt tacccccata tattgctcct caatccatat agatctcagt
     2401 accatagctc tgtcagagca aagaaaatct taactaatac aagttaagat ttttaatatc
     2461 cactcctcat ccctacattc caggtgatgg tggcgtccac tcattatcct gaactggagc
     2521 ataagatgcg ccatgccccc cggatatgct tttctgttgc ctgatatatt cgtggtagta
     2581 ctgttcacta cgctgacgat gtaaatccac atccgggcgg ctgattacaa agtgtccact
     2641 tccggttatt ccagctgcat tatgccaggc caccatttcc gcaaatacat cattaaactg
     2701 ttgatatgag ccttccggtt caaaacccat tcttggctca cgatcaaaca atacaggagc
     2761 aatcagattc agcttatcca ccagttcatt tctgtcctga tgatggtaga acaccacacg
     2821 gtccccataa tccacacaga ctaactcgcc attcctccga tcacgaaaac gcacactgcc
     2881 atctttctgc agacgggctt cgagaacgcc agtgtcttca taaagtggag ttcctcccgg
     2941 ttcacagaga atcacgcagc ggtcggcatt cgttgttcgt cgcttgtctt tgcgacgatc
     3001 acgataatcc cagccacgta actgtgataa tgcagctctg tctccctgta ccgcctgttg
     3061 ctccacccac tgccggtagg acaaaggata ccacttaccc tccgcaatca gtgacaaccg
     3121 ctcttccttc acactctcct tcagcctgat cagcgcctgc atacgctgca cttctgcaat
     3181 atggtaatgc agcttacgca attgtggatc ccggaactgc acacggatat acgccttgcg
     3241 ccgacggcag gcagcatgaa tttcccgtaa ccgttctccg taacgtaagt cgggtttacg
     3301 ccagtgctcc ttccatgcaa gataacgcgc gcgcaaatct tcccgcgcag ctgcacgggc
     3361 ttcacggcgt tcacgacgca gtacaggatc gcgccggaag ccgggctcca cttcatcgct
     3421 ggcagcaagt tccggattgt agcgacattc aggcttcacc cgttcaaata tatctgcacc
     3481 tgcaatctca aatggccctg cctgcggctc agccctggat aacgtcaaat ccgggtggat
     3541 actgctggcc ttcactggtg tgagctcatg attaaacgca tccacaatca caagaccatg
     3601 atgctgtttc tgcaacataa gccctttacg cgcaaacatc tcatggcacg cctgccagct
     3661 gaaattcccg cccgcgatca tatcttcaat caacgactca cactgaacag tcgtgtaccc
     3721 ccacagactt tccttatcgg aaaacagttc gcgctttatt gctccctgtg gacctgtccc
     3781 catccgaata cgataccatt cgtaatcact gacagcctga gtaccaagat tacgtccccg
     3841 gaatcgttct ccgggtataa cctgagtaaa aatatccttc ggtacaggct gccatccacc
     3901 atccagatcc tgcatataat ccagcgtcat taacggccac acactgtctg cacgtactgg
     3961 tgtctgttgt ttatcaaatc catcatgcag taccagatgc ccgtgctgga tccccatcca
     4021 taatccagcc tttgccagag ttctgtgcac atccaggcaa ctttccagct caccatttct
     4081 cgccatttcc ggtaagcgtt cggcaaaatg acggcaggcg tactgtttca gactctccgt
     4141 ttctgccact ttttccggcc ggatatcaac gtttatggct tccggatcat aacgaccggg
     4201 tgtccccacc tgactgaaaa tatctgtcgg tacaggctga tattccccca gtttccgccc
     4261 cagtttttca gctgtccatg acggtccgaa cgaactgagt gcaacacctt cccgcgctct
     4321 gtcccagcca tccttcacca ccagttcccc ttcctgcatc gtgatataca gaccatcact
     4381 ggccagccgt ttatgcaaag acaaccagtc ttgtgcgggc tcttcacgca gcccggcaat
     4441 cgacatctgc gcaatatatt ccctgaacgt ctgctttttc ccgcgtcgcc aggcattacg
     4501 gcgctcacgt accagcgctg ttttgcgcac aatacggttg ccgggtgcat gcacaaagca
     4561 gccattatca ggcgcgaatc cgtgcttaag ttcaagttca cgacatgcac ggctgagttt
     4621 ttcctggctc caggaaagcc agttaatata accagtttca ggatgtacac gattcacggc
     4681 aacatgcaca tgcagattat ccgtgtccgt atgaacggca gccacgtact gatgttttga
     4741 gagttcaaga gatttcagtg tgtgacgcac acagtcaaaa agctgctcag gacgcgggct
     4801 ttcatgcgca ggccaggaaa gaatgtagtg gaaaacgggg tctgtatcat tacgggaaaa
     4861 aacagctttg tccgccttgt actgcatctc ttcagcagct gtttccagtg aattgcagtt
     4921 atggaaacag gttacgccat agaaatttac ccactgactg ccgtccttca tcacatcaat
     4981 cagggagaca aatttttcgt tacgcaggcg tgttgcataa tcaacaagcc gtctgaaacg
     5041 gttgcggtga gggacatcac tcttgatttc gctgtcttct gtgagctcat tttcctgcac
     5101 atcatcccgc acagacgtgt acgcaatcag gtcttcaaag gaagacctgc cgtcacgtct
     5161 tttgtgcgga ataatggcat tcatcagcag ttatctcagc gtcccattaa gtctcttcgc
     5221 agagcattga catattcagt aatggcaaca agaacctctg catactcctt atcaccagtg
     5281 cgttttccct ccacaaacag atgcttctga aggcggccaa gacgcataat ctcatcaatc
     5341 atacggtcgt cagtacggca gttaaccacc ttatccagtg aagaacttcg tataaatgtt
     5401 gaaaccgtct gtccacttga ctccgctttt tctcttacga tttcagcctc atcctccgtg
     5461 aatctcgccg tgaattttac aattctcttc ctgttctcac tgcccgttcg ggttttcttc
     5521 tcactcatgg tctgctcctt accggggtgt cggggcaaag ccctgaccag gtggggaatg
     5581 tctgagtgcg cgtgcgcggt ccgacattcc cacatcctgt cccgtttttc agtccattat
     5641 agcctgttat ttttgtgcat catagtctta tgcagacggc agaggcaggg cgcagccctg
     5701 ccaggagtca gtttttgatg ataaaatcag gcttcaacat taaacggaga gaaagtgcca
     5761 atggcgtcta aaaaatttta ctcagatgat gacatccagc tggcaaaagc tgctttatcg
     5821 gaattgccag acctcaccgc tcaacggaaa acattgcgtg attttcttga tgccattcgt
     5881 gatgacatca ttatcctggt aaggacaaag ggttatacac tgacagatgt tcgtgacacg
     5941 ctcaaaaatg caggatatga agtaggagag aaagcacttc gtgacattat tcgggaagca
     6001 gaatcgaaaa aaccatcccg ccgttcttcc tctaaaacag cacctaaaaa gacagactca
     6061 ggcaggaaag atggcatcga tatgaacaac aattgatgtg acaatgtcac attgtgttct
     6121 gagattatga tgattacaga aaaaaaagcc ccggcataaa tacctgggct tttctgttta
     6181 atcagactcg tggcggcaat gcaccaggat aatgccgtgc aataatatca ttcagttttt
     6241 caacaaggtc agaacgctta aagacaatgt gtacggtccc tttctggtag ccacgcaccg
     6301 taaaatattc cccttctaac agcttaccga cattgtcacc gttcccgaga aattcaccat
     6361 actgcgcccc gatgctgttc ctgaagtcag gaacgttgcg cccctccagc agataaaacg
     6421 gacgtaccag gtcatccagt gcctcccgtc cgctgcagtc cagcgtcaca cgcccgttac
     6481 tccagcggta cagtaggttt tcaataatta ttttcttacc cagacggcag ggattattgg
     6541 ttctgtaatc ccatgacaat ttccggtaga catcaatcag cccctgttca aacgtctgca
     6601 tcttgctggc attaagatgg cggaatgttg ccagtacatt gtccagggtg atttcaggac
     6661 aggtgtcgct catcagctgg ctgttccact catcacgctg gcatgaactc atgaaggtgt
     6721 acatgcccgt atctgtcatc aggcggtccc agattttccg gtcggcaaga cgggtgatat
     6781 tccttttcgc ttcttccgtc ttatccatgc gacgaacgga atcagtcacg cagtcttcaa
     6841 acccgtaaag aaaaatatca cctgacgcat cgagcagaat cttgcgcgct gtcgccaggc
     6901 attccatcgc ctgcatgaac agtgcaatac cttcgttacg ctgcgcgatg atgcggtcga
     6961 tagctacaga cggaataaca tctgtcgtgc cggcagtatt caggtcagca gtgggtaaag
     7021 tctgattcat cacattgtct cctcatcgtc acgactgccg ggtcagccag gtcatttccc
     7081 ggaggagcga agctccttct gccgtccggg tcttcgggga tttccccgca gtggaccttt
     7141 ccgacggggg acgctcacca cgtcacgggg cagttgtccg ggggcagacg caaaagtttt
     7201 tga
//
LOCUS       DAJTVE010000424         5236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.guided.207, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000424 DAJTVE010000000
VERSION     DAJTVE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.guided.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..691)
                     /locus_tag="OO439_004512"
     CDS             complement(<1..691)
                     /locus_tag="OO439_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCR6318669.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKL"
     gene            952..1410
                     /gene="gpt"
                     /locus_tag="OO439_004513"
     CDS             952..1410
                     /gene="gpt"
                     /locus_tag="OO439_004513"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6318670.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1502..2746
                     /gene="frsA"
                     /locus_tag="OO439_004514"
     CDS             1502..2746
                     /gene="frsA"
                     /locus_tag="OO439_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6318671.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2804..3205
                     /gene="crl"
                     /locus_tag="OO439_004515"
     CDS             2804..3205
                     /gene="crl"
                     /locus_tag="OO439_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6318672.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3244..4293)
                     /gene="phoE"
                     /locus_tag="OO439_004516"
     CDS             complement(3244..4293)
                     /gene="phoE"
                     /locus_tag="OO439_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6318673.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            4581..>5236
                     /gene="proB"
                     /locus_tag="OO439_004517"
     CDS             4581..>5236
                     /gene="proB"
                     /locus_tag="OO439_004517"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6318674.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     "
BASE COUNT         1288 a         1290 c         1305 g         1353 t
ORIGIN      
        1 gcagtttgtt ggcattaccc aggccaacgt ggatttcccc gccggagtga ccccctttca
       61 gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca cgatctaaat
      121 gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca ccttcttctt
      181 cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag ccgaacgcac
      241 cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg tgaaccacgt
      301 tttcgtcagc cagaaccgcc agcgcagacg ccataccaat gccgttatct gcacccagcg
      361 tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga
      421 agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga
      481 ccgatttacg attttccata cctgcggtag caggtttacg aatcaggata ttacctacct
      541 gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt
      601 gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg
      661 gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa
      721 acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag
      781 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
      841 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
      901 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
      961 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     1021 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggt
     1081 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     1141 gatcacgaca accagcgcga gcttaaagtg ctaaaacgcg cagaaggcga tggcgaaggc
     1201 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     1261 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     1321 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     1381 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     1441 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     1501 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     1561 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     1621 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     1681 cattgaccca cgtgaaatcc tcgacgtcca ggcgcgtatt gtgatgagcg atgccgaacg
     1741 taccgacgat gatttatacg atacggtgat tggctaccgt ggtggcaact ggatttatga
     1801 gtgggccatc caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag atccgcaact
     1861 cagtggtcgt cactggctgc atgcggctac gttgtacaac attgctgcct atcctcatct
     1921 gaaaggagat gacctggccg agcaagcgca ggctttgtca aaccgtgcct atgaagaagc
     1981 cgcccagcgt ctgccgggca cgatgcggca gatggagttt accgtacccg gcggtgcgcc
     2041 catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg
     2101 tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc
     2161 gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttcat caaaatggaa
     2221 gctcacccag gactccagcc tgttgcatca gcacgtttta aaggctctgc ctaacgtacc
     2281 gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt
     2341 gcgtctggca tatcttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt
     2401 tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt
     2461 tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa
     2521 tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc tgcccaacgc caatgttatc
     2581 aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc
     2641 atctgctgac ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa
     2701 aggtcttcag gaaatcaccg gctggatcga aaaacgcttg tgttaaaaat ttgctaaaat
     2761 ttgccaattt ggtaaaacag ttgcatcaca acaggagatt gcaatgacgt taccgagtgg
     2821 acacccgaag agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg
     2881 taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc
     2941 ggcaccggaa gtgcgtgagt tctggggctg gtggatggag cttgaagcgc aggaatcccg
     3001 ttttacctac agttaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc
     3061 ggtaaaagac actgaagtgg ttgaacgact ggagcacacc ctacgtgagt ttcacgagaa
     3121 gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa ccggcggatg attttcgtga
     3181 tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga tgtatcacat ccggcaatat
     3241 ccattaaaac tgataggtca tgccaaccgc gacaatatca tcattattaa tattcaattt
     3301 gttatcgcta tccagttggt tgattttata atcaacaaac gctgacatat ttttgttgaa
     3361 ataatacgta gcaccgacgt cgatataatt gaccagatct tcatcaccga taccttcaat
     3421 atctttccct ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta
     3481 ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat
     3541 tttgcgtgtt tcagaataga aagttgccag ataaatatta ttggcatcgt atttcagacc
     3601 tgttgcccat gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt
     3661 gcgatctgag ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt
     3721 caatgacgtg ccgaagccat cgccgttttg ctttttaacg tcgcggtttt cgtttttccc
     3781 ttgatattgc agggttaagt tcaggccatc gataacgccg aagaagtcgg tgttccgata
     3841 cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg gtctgcgccg aggaatcgcc
     3901 accaaattcc gggaacatat cggtccaggc ttccacgtca tacaacgcgc ccaggttacg
     3961 accataatcg aaagaaccca aatctttata tttcaacccg gcaaaagcga gacgcgtttt
     4021 ctgctgtgca gtatcactct ccgctttatt tccggcaaac tccgcttccc accgaccata
     4081 accagtcagt tgatcgttaa tttgtgtttc acctttaaaa ccaaaacgga tataactctg
     4141 gtcgccatct ttactggcgt tatcactcat ataatgcata actttgccat agacatccag
     4201 tttattaccg tctttattat atatttctgc agcctgtaca gatgcagatg ccacaatgcc
     4261 catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc
     4321 gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa
     4381 gatatattac agattaataa tattcttaaa atgtggtaat ttattaaatc tgtaataaaa
     4441 gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgctcg
     4501 cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta
     4561 aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca
     4621 gtgtgctaac aggcggatcg cgccgtctga accgtgccca tattgttgaa cttgttcgcc
     4681 agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg
     4741 ccgccggacg tgagcacctg ggttacccgg aactgccagc gactatcgcc tcgaaacaac
     4801 tgctggcagc ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt
     4861 atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct
     4921 tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca
     4981 atgagaacga tgctgtcgct acggcagaga ttaaagtcgg cgataacgat aacctttctg
     5041 cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt
     5101 tgtataccgc tgacccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca
     5161 ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca
     5221 tgagtaccaa attgca
//
LOCUS       DAJTVE010000425         4638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000425 DAJTVE010000000
VERSION     DAJTVE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.guided.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..296
                     /gene="frdD"
                     /locus_tag="OO439_004518"
     CDS             <1..296
                     /gene="frdD"
                     /locus_tag="OO439_004518"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6318675.1"
                     /translation="MWSAIIAPVMILLVGILLPLGLFPGDALSYERVLAFAQSFIGRV
                     FLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGIVTI"
     gene            359..1492
                     /locus_tag="OO439_004519"
     CDS             359..1492
                     /locus_tag="OO439_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6318676.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1581..2114
                     /gene="blc"
                     /locus_tag="OO439_004520"
     CDS             1581..2114
                     /gene="blc"
                     /locus_tag="OO439_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6318677.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2111..2428)
                     /gene="sugE"
                     /locus_tag="OO439_004521"
     CDS             complement(2111..2428)
                     /gene="sugE"
                     /locus_tag="OO439_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6318678.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2603..2749)
                     /gene="ecnB"
                     /locus_tag="OO439_004522"
     CDS             complement(2603..2749)
                     /gene="ecnB"
                     /locus_tag="OO439_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6318679.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2860..2985)
                     /gene="ecnA"
                     /locus_tag="OO439_004523"
     CDS             complement(2860..2985)
                     /gene="ecnA"
                     /locus_tag="OO439_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6318680.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3037..3603)
                     /gene="efp"
                     /locus_tag="OO439_004524"
     CDS             complement(3037..3603)
                     /gene="efp"
                     /locus_tag="OO439_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6318681.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3645..>4638
                     /gene="epmB"
                     /locus_tag="OO439_004525"
     CDS             3645..>4638
                     /gene="epmB"
                     /locus_tag="OO439_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6318682.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPS"
BASE COUNT         1149 a         1183 c         1185 g         1121 t
ORIGIN      
        1 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
       61 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
      121 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
      181 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
      241 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
      301 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
      361 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
      421 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
      481 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
      541 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
      601 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
      661 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
      721 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
      781 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
      841 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
      901 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
      961 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     1021 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     1081 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     1141 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     1201 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     1261 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     1321 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     1381 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     1441 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     1501 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     1561 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     1621 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     1681 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     1741 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     1801 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     1861 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     1921 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     1981 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     2041 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     2101 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     2161 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     2221 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     2281 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     2341 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     2401 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     2461 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     2521 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     2581 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     2641 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     2701 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     2761 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     2821 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     2881 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     2941 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     3001 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     3061 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     3121 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     3181 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     3241 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     3301 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     3361 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     3421 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     3481 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     3541 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     3601 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     3661 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     3721 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     3781 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     3841 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     3901 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     3961 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     4021 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     4081 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     4141 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     4201 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     4261 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     4321 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     4381 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     4441 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     4501 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     4561 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     4621 ttggcggcga acccagca
//
LOCUS       DAJTVE010000426         3002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.guided.266, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000426 DAJTVE010000000
VERSION     DAJTVE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.guided.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            992..1807
                     /gene="sul2"
                     /locus_tag="OO439_004526"
     CDS             992..1807
                     /gene="sul2"
                     /locus_tag="OO439_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6318683.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1868..2395
                     /locus_tag="OO439_004527"
                     /pseudo
     CDS             1868..2395
                     /locus_tag="OO439_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2416..2889
                     /locus_tag="OO439_004528"
     CDS             2416..2889
                     /locus_tag="OO439_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6318684.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          744 a          751 c          759 g          748 t
ORIGIN      
        1 gtgataacag atgtctggaa atataggggc aaatccaacc ggatctaact gcatccccgc
       61 cccataaaaa aaatgtcgct cagacaccat caggtggaaa acaagagagt cgtaaaaaaa
      121 gattctgata acacctgtat ggctgatttt aataacaacc tttacgcgat tattaatctc
      181 ctccgctcac catagtaagc ggaggattca ggatttcaat ccatcaccag aggtacaata
      241 aatgtgtatt agtagcccgg aaacaaactc atggtctgtt atttatcgta aaaattccgg
      301 agaagatatc aatatcactt ccctgacgtt taaaaatagt cttctggcag cccggacact
      361 gatggtgccc gaaaactata tgatatgcat actcagaaac ggggagaggg tcagacgatg
      421 ggacagagaa atacttgcag gctcaaacag atggtacaag tgttctcccg ataatttcga
      481 aatactgggt aagctcccca taataaataa agtcacaaaa ctgataaaat cataatctac
      541 ggattttaat cagttaagct aaatcaggat gaacgctttg attatgaatt atacatgaaa
      601 acaaatatgg gcagcacaaa aaatggtgcc agggttcccc acgttcgtaa tagattcact
      661 tctatggata ttggtatgaa ttcccagatc ccactctatt gtaaacaaga catttttatc
      721 ttttatattc aatggcttat tttcctgcta attggtaata ccatgaaaaa taccatgctc
      781 agaaaaggct taacaatatt ttgaaaaatt gcctactgag cgctgccgca cagctccata
      841 ggccgctttc ctggctttgc ttccagatgt atgctattct gctcctgcag ctaatggatc
      901 accgcaaaca ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgagactc
      961 atgctcgatt attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat
     1021 cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc
     1081 cattgcgcag gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc
     1141 atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc
     1201 ggtgctggac gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc
     1261 gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc
     1321 agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc
     1381 ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat
     1441 tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg
     1501 ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc
     1561 ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt
     1621 ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc
     1681 gacactcgct gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga
     1741 gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat
     1801 tcgttaactg cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga
     1861 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1921 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1981 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     2041 caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc
     2101 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     2161 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     2221 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     2281 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     2341 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgttaa
     2401 cccaggatga gaaccttgaa agtatcattg atagctgcga aagcgaaaaa cggcgtgatt
     2461 ggttgcggtc cagacatacc ctggtccgcg aaaggggagc agctactttt taaagcattg
     2521 acctacaatc agtggcttct ggtgggtcgc aagacgtttg aatctatggg cgcactcccc
     2581 aataggaaat acgcggtcgt tacccgctca ggttggacat caaatgatga caatgtagtt
     2641 gtatttcagt caatcgaaga ggccatggac aggctagctg aattcaccgg tcacgttata
     2701 gtgtctggtg gcggagaaat ttaccgagaa acattaccca tggcctctac gctccactta
     2761 tcgacgatcg acatcgagcc agagggggat gttttcttcc cgagtattcc aaataccttc
     2821 gaagttgttt ttgagcaaca ctttacttca aacattaact attgctatca aatttggaaa
     2881 aagggttaac aaagctatgc aatcgacggc aaaaagcttc gttcgcttcg cgcactacgc
     2941 ctttttccgc gattgatagc gacgatatgg ttgtttgcca tggtgatccc tgcatgccga
     3001 ac
//
LOCUS       DAJTVE010000427         2322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466792-rid15818293.guided.283, whole genome shotgun
            sequence.
ACCESSION   DAJTVE010000427 DAJTVE010000000
VERSION     DAJTVE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466792
            Sequence Read Archive: ERR10074658
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477324.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:37:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,442
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 115 of 245
            Pseudo Genes (incomplete)         :: 64 of 245
            Pseudo Genes (internal stop)      :: 89 of 245
            Pseudo Genes (multiple problems)  :: 23 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466792-rid15818293.guided.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-10-03"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..786
                     /locus_tag="OO439_004529"
     CDS             313..786
                     /locus_tag="OO439_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6318685.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            881..1405
                     /gene="sat2"
                     /locus_tag="OO439_004530"
     CDS             881..1405
                     /gene="sat2"
                     /locus_tag="OO439_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6318686.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1463..2251
                     /gene="aadA1"
                     /locus_tag="OO439_004531"
     CDS             1463..2251
                     /gene="aadA1"
                     /locus_tag="OO439_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6318687.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          647 a          518 c          574 g          583 t
ORIGIN      
        1 aactgtctgc ctatacagcc tattctagct gggatttaaa aaagtgcctg ttttttacgc
       61 ctagagatgc ttgtttaccg gtagagtttt aatttaatgc taaataaatt aaaatgttat
      121 gagttctttg ggtgagataa tgtgcatcgt gcaagcagga tagacggcat gcacgatttg
      181 taataacaga gtgtcttgta tttttaaaga aagtctattt aatacaagtg attatattaa
      241 ttaacggtaa gcatcagcgg gtgacaaaac gagcatgctt actaataaaa tgttaacctc
      301 tgaggaagaa ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg
      361 aatggccctg atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc
      421 tataaccaat ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac
      481 cgaaagtatg cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc
      541 tttccatcaa ttaaagatgc tttaaccaac ctaaagaaaa taacggatca tgtcattgtt
      601 tcaggtggtg gggagatata caaaagcctg atcgatcaag tagatacact acatatatct
      661 acaatagaca tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg
      721 ccagttttta cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag
      781 ggttaacaag tggcagcaac ggattcgcaa acctgtcacg ccttttgtac caaaagccgc
      841 gccaggtttg cgatccgctg tgccaggcgt taggcgtcat atgaagattt cggtgatccc
      901 tgagcaggtg gcggaaacat tggatgctga gaaccatttc attgttcgtg aagtgttcga
      961 tgtgcaccta tccgaccaag gctttgaact atctaccaga agtgtgagcc cctaccggaa
     1021 ggattacatc tcggatgatg actctgatga agactctgct tgctatggcg cattcatcga
     1081 ccaagagctt gtcgggaaga ttgaactcaa ctcaacatgg aacgatctag cctctatcga
     1141 acacattgtt gtgtcgcaca cgcaccgagg caaaggagtc gcgcacagtc tcatcgaatt
     1201 tgcgaaaaag tgggcactaa gcagacagct ccttggcata cgattagaga cacaaacgaa
     1261 caatgtacct gcctgcaatt tgtacgcaaa atgtggcttt actctcggcg gcattgacct
     1321 gttcacgtat aaaactagac ctcaagtctc gaacgaaaca gcgatgtact ggtactggtt
     1381 ctcgggagca caggatgacg cctaacaatt cattcaagcc gacaccgctt cgcggcgcgg
     1441 cttaattcag gagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1501 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1561 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1621 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1681 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1741 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1801 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1861 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1921 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     1981 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2041 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     2101 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctaggcagg cttatcttgg
     2161 acaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa
     2221 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
     2281 cttcgcggcg cggcttaact caagcgttag agagctgggg aa
//
